Magma wagging and whirling in volcanic conduits

NASA Astrophysics Data System (ADS)

Liao, Yang; Bercovici, David; Jellinek, Mark

2018-02-01

Seismic tremor characterized by 0.5-7 Hz ground oscillations commonly occur before and during eruptions at silicic volcanoes with widely ranging vent geometries and edifice structures. The ubiquitous characteristics of this tremor imply that its causes are potentially common to silicic volcanoes. Here we revisit and extend to three dimensions the magma-wagging model for tremor (Jellinek and Bercovici, 2011; Bercovici et al., 2013), wherein a stiff magma column rising in a vertical conduit oscillates against a surrounding foamy annulus of bubbly magma, giving rise to tremor. While prior studies were restricted to two-dimensional lateral oscillations, here we explore three-dimensional motion and additional modes of oscillations. In the absence of viscous damping, the magma column undergoes 'whirling' motion: the center of each horizontal section of the column traces an elliptical trajectory. In the presence of viscous effect we identify new 'coiling' and 'uncoiling' column bending shapes with relatively higher and comparable rates of dissipation to the original two-dimensional magma wagging model. We also calculate the seismic P-wave response of the crustal material around the volcanic conduit to the new whirling motions and propose seismic diagnostics for different wagging patterns using the time-lag between seismic stations. We test our model by analyzing pre-eruptive seismic data from the 2009 eruption of Redoubt Volcano. In addition to suggesting that the occurrence of elliptical whirling motion more than 1 week before the eruption, our analysis of seismic time-lags also implies that the 2009 eruption was accompanied by qualitative changes in the magma wagging behavior including fluctuations in eccentricity and a reversal in the direction of elliptical whirling motion when the eruption was immediately impending.

Changes in mesenteric, renal, and aortic flows with +Gx acceleration

NASA Technical Reports Server (NTRS)

Stone, H. L.; Erickson, H. H.; Sandler, H.

1974-01-01

Previous studies in man and dogs have indicated that the splanchnic bed might contribute to the maintenance of arterial pressure during +Gx acceleration. Eight mongrel dogs were chronically instrumented with Doppler flow probes around the superior mesenteric (SMA) and renal arteries (RA) as well as the terminal aorta (TA). A solid-state pressure transducer was placed in the aorta distal to the flow probe. Using alpha-chloralose anesthesia following a 2-4 week recovery period, the animals were subjected to 120 sec at levels of 5, 10 and 15 +Gx acceleration on a 7.6-m radius centrifuge. The results indicate that both an active component and a mechanical component contribute to the maintenance of arterial pressure during +Gx acceleration.

CORRELATIONS IN HORIZONTAL BRANCH OSCILLATIONS AND BREAK COMPONENTS IN XTE J1701-462 AND GX 17+2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bu, Qing-cui; Chen, Li; Zhang, Liang

2015-01-20

We studied the horizontal branch oscillations (HBO) and the band-limited components observed in the power spectra of the transient neutron star low-mass X-ray binary XTE J1701-462 and the persistent ''Sco-like'' Z source GX 17+2. These two components were studied based on the state-resolved spectra. We found that the frequencies of XTE J1701-462 lie on the known correlations (WK and PBK), showing consistency with other types of X-ray binaries (black holes, atoll sources, and millisecond X-ray pulsars). However, GX 17+2 is shifted from the WK correlation like other typical Z sources. We suggest that the WK/PBK main track forms a boundarymore » that separates persistent sources from transient sources. The characteristic frequencies of break and HBO are independent of accretion rate in both sources, though it depends on spectral models. We also report the energy dependence of the HBO and break frequencies in XTE J1701-462 and how the temporal properties change with spectral state in XTE J1701-462 and GX 17+2. We studied the correlation between rms at the break and the HBO frequency. We suggest that HBO and break components for both sources probably arise from a similar physical mechanism: Comptonization emission from the corona. These two components could be caused by the same kind of oscillation in a corona with uneven density, and they could be generated from different areas of the corona. We further suggest that different proportions of the Comptonization component in the total flux cause the different distribution between GX 17+2 and XTE J1701-462 in the rms{sub break}-rms{sub HBO} diagram.« less

Oxidative Stress and Nano-Toxicity Induced by TiO2 and ZnO on WAG Cell Line

PubMed Central

Dubey, Akhilesh; Goswami, Mukunda; Yadav, Kamalendra; Chaudhary, Dharmendra

2015-01-01

Metallic nanoparticles are widely used in cosmetics, food products and textile industry. These particles are known to cause respiratory toxicity and epithelial inflammation. They are eventually released to aquatic environment necessitating toxicity studies in cells from respiratory organs of aquatic organisms. Hence, we have developed and characterized a new cell line, WAG, from gill tissue of Wallago attu for toxicity assessment of TiO2 and ZnO nanoparticles. The efficacy of the cell line as an in vitro system for nanoparticles toxicity studies was established using electron microscopy, cytotoxicity assays, genotoxicity assays and oxidative stress biomarkers. Results obtained with MTT assay, neutral red uptake assay and lactate dehydrogenase assay showed acute toxicity to WAG cells with IC50 values of 25.29±0.12, 34.99±0.09 and 35.06±0.09 mg/l for TiO2 and 5.716±0.1, 3.160±0.1 and 5.57±0.12 mg/l for ZnO treatment respectively. The physicochemical properties and size distribution of nanoparticles were characterized using electron microscopy with integrated energy dispersive X-ray spectroscopy and Zetasizer. Dose dependent increase in DNA damage, lipid peroxidation and protein carbonylation along with a significant decrease in activity of Superoxide Dismutase, Catalase, total Glutathione levels and total antioxidant capacity with increasing concentration of exposed nanoparticles indicated that the cells were under oxidative stress. The study established WAG cell line as an in vitro system to study toxicity mechanisms of nanoparticles on aquatic organisms. PMID:26011447

Proepileptic patterns in EEG of WAG/Rij rats

NASA Astrophysics Data System (ADS)

Grubov, Vadim V.; Sitnikova, Evgenia Yu.; Nedaivozov, Vladimir O.; Koronovskii, Alexey A.

2018-04-01

In this paper we study specific oscillatory patterns on EEG signals of WAG/Rij rats. These patterns are known as proepileptic because they occur in time period during the development of absence-epilepsy before fully-formed epileptic seizures. In the paper we analyze EEG signals of WAG/Rij rats with continuous wavelet transform and empirical mode decomposition in order to find particular features of epileptic spike-wave discharges and nonepileptic sleep spindles. Then we introduce proepileptic activity as patterns that combine features of epileptic and non-epileptic activity. We analyze proepileptic activity in order to specify its features and time-frequency structure.

WAG 2 remedial investigation and site investigation site-specific work plan/health and safety checklist for the soil and sediment task. Environmental Restoration Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Holt, V.L.; Burgoa, B.B.

1993-12-01

This document is a site-specific work plan/health and safety checklist (WP/HSC) for a task of the Waste Area Grouping 2 Remedial Investigation and Site Investigation (WAG 2 RI&SI). Title 29 CFR Part 1910.120 requires that a health and safety program plan that includes site- and task-specific information be completed to ensure conformance with health- and safety-related requirements. To meet this requirement, the health and safety program plan for each WAG 2 RI&SI field task must include (1) the general health and safety program plan for all WAG 2 RI&SI field activities and (2) a WP/HSC for that particular field task.more » These two components, along with all applicable referenced procedures, must be kept together at the work site and distributed to field personnel as required. The general health and safety program plan is the Health and Safety Plan for the Remedial Investigation and Site Investigation of Waste Area Grouping 2 at the Oak Ridge National Laboratory, Oak Ridge, Tennessee (ORNL/ER-169). The WP/HSCs are being issued as supplements to ORNL/ER-169.« less

The infrared counterpart of GX 13 + 1

NASA Technical Reports Server (NTRS)

Garcia, M. R.; Grindlay, J. E.; Bailyn, C. D.; Pipher, J. L.; Shure, M. A.; Woodward, C. E.

1992-01-01

A bright (K = 12) IR source is discovered which is likely the counterpart to the bright galactic-bulge X-ray source GX 13 + 1. Observations with the MMT IR photometer and the Rochester IR Array camera at the IRTF allow determination of the source position to about 0.7 arcsec, allow the IR colors to be measured, and show no variability on a 1-yr timescale. Four possible sources for the IR emission are considered and it is most likely due to a K-giant secondary. The discovery of a late-type giant secondary in GX 13 + 1 is contrary to the expectation that low-mass X-ray binaries which show quasi-periodic oscillations (QPO) have giant companions, while those which do not show QPO (like GX 13 + 1) have dwarf secondaries. The relation between the size of the scattered X-ray halo and the Av inferred from the IR observations is compared to that found in other X-ray sources.

Sm@C2v(3)-C80: site-hopping motion of endohedral Sm atom and metal-induced effect on redox profile

NASA Astrophysics Data System (ADS)

Xu, Wei; Niu, Ben; Shi, Zujin; Lian, Yongfu; Feng, Lai

2012-10-01

A new metallofullerene Sm@C2v(3)-C80 was synthesized and characterized. X-Ray analysis showed that the endohedral Sm atom undergoes a hopping motion between several off-center sites, even at low temperature. In addition, a comparative electrochemical study between Sm@C2v(3)-C80 and Yb@C2v(3)-C80 revealed their different redox potentials, suggesting a metal-induced effect on their redox profiles.A new metallofullerene Sm@C2v(3)-C80 was synthesized and characterized. X-Ray analysis showed that the endohedral Sm atom undergoes a hopping motion between several off-center sites, even at low temperature. In addition, a comparative electrochemical study between Sm@C2v(3)-C80 and Yb@C2v(3)-C80 revealed their different redox potentials, suggesting a metal-induced effect on their redox profiles. CCDC reference number 894168. For crystallographic data in CIF or other electronic format see DOI: 10.1039/c2nr32193a

A method to associate all possible combinations of genetic and environmental factors using GxE landscape plot.

PubMed

Nagaie, Satoshi; Ogishima, Soichi; Nakaya, Jun; Tanaka, Hiroshi

2015-01-01

Genome-wide association studies (GWAS) and linkage analysis has identified many single nucleotide polymorphisms (SNPs) related to disease. There are many unknown SNPs whose minor allele frequencies (MAFs) as low as 0.005 having intermediate effects with odds ratio between 1.5~3.0. Low frequency variants having intermediate effects on disease pathogenesis are believed to have complex interactions with environmental factors called gene-environment interactions (GxE). Hence, we describe a model using 3D Manhattan plot called GxE landscape plot to visualize the association of p-values for gene-environment interactions (GxE). We used the Gene-Environment iNteraction Simulator 2 (GENS2) program to simulate interactions between two genetic loci and one environmental factor in this exercise. The dataset used for training contains disease status, gender, 20 environmental exposures and 100 genotypes for 170 subjects, and p-values were calculated by Cochran-Mantel-Haenszel chi-squared test on known data. Subsequently, we created a 3D GxE landscape plot of negative logarithm of the association of p-values for all the possible combinations of genetic and environmental factors with their hierarchical clustering. Thus, the GxE landscape plot is a valuable model to predict association of p-values for GxE and similarity among genotypes and environments in the context of disease pathogenesis. GxE - Gene-environment interactions, GWAS - Genome-wide association study, MAFs - Minor allele frequencies, SNPs - Single nucleotide polymorphisms, EWAS - Environment-wide association study, FDR - False discovery rate, JPT+CHB - HapMap population of Japanese in Tokyo, Japan - Han Chinese in Beijing.

Effect of acute exposure to hypergravity (GX vs. GZ) on dynamic cerebral autoregulation

NASA Technical Reports Server (NTRS)

Serrador, J. M.; Wood, S. J.; Picot, P. A.; Stein, F.; Kassam, M. S.; Bondar, R. L.; Rupert, A. H.; Schlegel, T. T.

2001-01-01

We examined the effects of 30 min of exposure to either +3GX (front-to-back) or +GZ (head-to-foot) centrifugation on cerebrovascular responses to 80 degrees head-up tilt (HUT) in 14 healthy individuals. Both before and after +3 GX or +3 GZ centrifugation, eye-level blood pressure (BP(eye)), end tidal PCO2 (PET(CO2)), mean cerebral flow velocity (CFV) in the middle cerebral artery (transcranial Doppler ultrasound), cerebral vascular resistance (CVR), and dynamic cerebral autoregulatory gain (GAIN) were measured with subjects in the supine position and during subsequent 80 degrees HUT for 30 min. Mean BP(eye) decreased with HUT in both the GX (n = 7) and GZ (n = 7) groups (P < 0.001), with the decrease being greater after centrifugation only in the GZ group (P < 0.05). PET(CO2) also decreased with HUT in both groups (P < 0.01), but the absolute level of decrease was unaffected by centrifugation. CFV decreased during HUT more significantly after centrifugation than before centrifugation in both groups (P < 0.02). However, these greater decreases were not associated with greater increases in CVR. In the supine position after centrifugation compared with before centrifugation, GAIN increased in both groups (P < 0.05, suggesting an autoregulatory deficit), with the change being correlated to a measure of otolith function (the linear vestibulo-ocular reflex) in the GX group (r = 0.76, P < 0.05) but not in the GZ group (r = 0.24, P = 0.60). However, GAIN was subsequently restored to precentrifugation levels during postcentrifugation HUT (i.e., as BP(eye) decreased), suggesting that both types of centrifugation resulted in a leftward shift of the cerebral autoregulation curve. We speculate that this leftward shift may have been due to vestibular activation (especially during +GX) or potentially to an adaptation to reduced cerebral perfusion pressure during +GZ.

SMC condensation centers in Bacillus subtilis are dynamic structures.

PubMed

Kleine Borgmann, Luise A K; Hummel, Hanna; Ulbrich, Maximilian H; Graumann, Peter L

2013-05-01

SMC and MukB complexes consist of a central SMC dimer and two essential binding partners, ScpA and ScpB (MukE and MukF), and are crucial for correct chromosome compaction and segregation. The complexes form two bipolar assemblies on the chromosome, one in each cell half. Using fluorescence recovery after photobleaching (FRAP), we provide evidence that the SMC complex has high exchange rates. This depends to a considerable degree on de novo protein synthesis, revealing that the bacterial SMC complex has high on and off rates for binding to the chromosome. A mutation in SMC that affects ATPase activity and results in exaggerated DNA binding in vitro causes a strong segregation defect in vivo and affects the localization of the entire SMC complex, which localizes to many more sites in the cell than under normal conditions. These data indicate that ATP turnover is important for the function of Bacillus subtilis SMC. In contrast, the centromere protein Spo0J and DNA gyrase showed much less exchange between distinct binding sites on the chromosome than that seen with SMC. Binding of Spo0J to the origin regions was rather static and remained partially conserved until the next cell cycle. Our experiments reveal that the SMC complex has a high, condensin-like turnover rate and that an alteration of the ATPase cycle affects SMC function in vivo, while several nucleoid-associated proteins feature limited or slow exchange between different sites on the nucleoid, which may be the basis for epigenetic-like phenomena observed in bacteria.

Research Review: Gene–environment interaction (GxE) research in youth depression – a systematic review with recommendations for future research

PubMed Central

Dunn, Erin C.; Uddin, Monica; Subramanian, S.V.; Smoller, Jordan W.; Galea, Sandro; Koenen, Karestan C.

2011-01-01

Background Depression is a major public health problem among youth, currently estimated to affect as many as 9% of US children and adolescents. The recognition that both genes (“nature”) and environments (“nurture”) are important for understanding the etiology of depression has led to rapid growth in research exploring gene–environment interactions (GxE). However, there has been no systematic review of GxE in youth depression to date. Methods The goal of this article was to systematically review evidence on the contribution of GxE to the risk of child and adolescent depression. Though a search of PubMed and PsycINFO databases to 1 April 2010, we identified 20 candidate gene–environment interaction studies focused on depression in youth (up to age 26) and compared each study in terms of the following characteristics: research design and sample studied; measure of depression and environment used; genes explored; and GxE findings in relation to these factors. Results In total 80% of studies (n=16) found at least one significant GxE association. However, there was wide variation in methods and analyses adopted across studies, especially with respect to environmental measures used and tests conducted to estimate GxE. This heterogeneity made it difficult to compare findings and evaluate the strength of the evidence for GxE. Conclusions The existing body of GxE research on depression in youth contains studies that are conceptually and methodologically quite different, which contributes to mixed findings and makes it difficult to assess the current state of the evidence. To decrease this heterogeneity, we offer 20 recommendations that are focused on: (1) reporting GxE research; (2) testing and reporting GxE effects; (3) conceptualizing, measuring, and analyzing depression; (4) conceptualizing measuring, and analyzing environment; (5) increasing power to test for GxE; and (6) improving the quality of genetic data used. Although targeted to GxE research on

[Comparative analysis of the maternal motivation expression in WAG/Rij and Wistar rats in the place preference and open field tests].

PubMed

Dobriakova, Iu V; Tanaeva, K K; Dubynin, V A; Sarkisova, K Iu

2014-01-01

Maternal behavior in females of WAG/Rij and Wistar rats was compared in the place preference test from 2 to 8 days after delivery, as well as in the open field test from 4 to 6 days after delivery. In females of WAG/Rij rats compared with females of Wistar rats weaker expression of maternal motivation has been revealed in both tests: they spend less time in the compartment associated with pups. Moreover, in females of WAG/Rij rats, number of approaches to pups, number of pup-carryings and time spent with pups (time of contacts) were less than in females of Wistar rats. Reduced maternal motivation in females of WAG/Rij rats in the place preference test persisted in repeated testing, while in the open field test it was detected only in the first testing, indicating higher reliability of the place preference test for revealing inter-strain differences in the expression of maternal motivation. It is supposed that weaker expression of maternal behavior and preference is due to hypo-function of the mesolimbic dopaminergic bran system in WAG/Rij rats as a genetic model of depression associated with absence epilepsy.

VizieR Online Data Catalog: OGLE II SMC eclipsing binaries (Wyrzykowski+, 2004)

NASA Astrophysics Data System (ADS)

Wyrzykowski, L.; Udalski, A.; Kubiak, M.; Szymanski, M. K.; Zebrun, K.; Soszinski, I.; Wozniak, P. R.; Pietrzynski, G.; Szewczyk, O.

2009-03-01

We present new version of the OGLE-II catalog of eclipsing binary stars detected in the Small Magellanic Cloud, based on Difference Image Analysis catalog of variable stars in the Magellanic Clouds containing data collected from 1997 to 2000. We found 1351 eclipsing binary stars in the central 2.4 square degree area of the SMC. 455 stars are newly discovered objects, not found in the previous release of the catalog. The eclipsing objects were selected with the automatic search algorithm based on the artificial neural network. The full catalog with individual photometry is accessible from the OGLE INTERNET archive, at ftp://sirius.astrouw.edu.pl/ogle/ogle2/var_stars/smc/ecl . Regular observations of the SMC fields started on June 26, 1997 and covered about 2.4 square degrees of central parts of the SMC. Reductions of the photometric data collected up to the end of May 2000 were performed with the Difference Image Analysis (DIA) package. (1 data file).

Complex rearranged small supernumerary marker chromosomes (sSMC), three new cases; evidence for an underestimated entity?

PubMed Central

Trifonov, Vladimir; Fluri, Simon; Binkert, Franz; Nandini, Adayapalam; Anderson, Jasen; Rodriguez, Laura; Gross, Madeleine; Kosyakova, Nadezda; Mkrtchyan, Hasmik; Ewers, Elisabeth; Reich, Daniela; Weise, Anja; Liehr, Thomas

2008-01-01

Background Small supernumerary marker chromosomes (sSMC) are present ~2.6 × 106 human worldwide. sSMC are a heterogeneous group of derivative chromosomes concerning their clinical consequences as well as their chromosomal origin and shape. Besides the sSMC present in Emanuel syndrome, i.e. der(22)t(11;22)(q23;q11), only few so-called complex sSMC are reported. Results Here we report three new cases of unique complex sSMC. One was a de novo case with a dic(13 or 21;22) and two were maternally derived: a der(18)t(8;18) and a der(13 or 21)t(13 or 21;18). Thus, in summary, now 22 cases of unique complex sSMC are available in the literature. However, this special kind of sSMC might be under-diagnosed among sSMC-carriers. Conclusion More comprehensive characterization of sSMC and approaches like reverse fluorescence in situ hybridization (FISH) or array based comparative genomic hybridization (array-CGH) might identify them to be more frequent than only ~0.9% among all sSMC. PMID:18471318

Potentiation of mGlu5 receptors with the novel enhancer, VU0360172, reduces spontaneous absence seizures in WAG/Rij rats

PubMed Central

D’Amore, V.; Santolini, I.; van Rijn, C.M.; Biagioni, F.; Molinaro, G.; Prete, A.; Conn, P.J.; Lindsley, C.W.; Zhou, Y.; Vinson, P.N.; Rodriguez, A.L.; Jones, C.K.; Stauffer, S.R.; Nicoletti, F.; van Luijtelaar, G.; Ngomba, R.T.

2013-01-01

Absence epilepsy is generated by the cortico-thalamo-cortical network, which undergoes a finely tuned regulation by metabotropic glutamate (mGlu) receptors. We have shown previously that potentiation of mGlu1 receptors reduces spontaneous occurring spike and wave discharges (SWDs) in the WAG/Rij rat model of absence epilepsy, whereas activation of mGlu2/3 and mGlu4 receptors produces the opposite effect. Here, we have extended the study to mGlu5 receptors, which are known to be highly expressed within the cortico-thalamo-cortical network. We used presymptomatic and symptomatic WAG/Rij rats and aged-matched ACI rats. WAG/Rij rats showed a reduction in the mGlu5 receptor protein levels and in the mGlu5-receptor mediated stimulation of polyphosphoinositide hydrolysis in the ventrobasal thalamus, whereas the expression of mGlu5 receptors was increased in the somatosensory cortex. Interestingly, these changes preceded the onset of the epileptic phenotype, being already visible in pre-symptomatic WAG/Rij rats. SWDs in symptomatic WAG/Rij rats were not influenced by pharmacological blockade of mGlu5 receptors with MTEP (10 or 30 mg/kg, i.p.), but were significantly decreased by mGlu5 receptor potentiation with the novel enhancer, VU0360172 (3 or 10 mg/kg, s.c.), without affecting motor behaviour. The effect of VU0360172 was prevented by co-treatment with MTEP. These findings suggest that changes in mGlu5 receptors might lie at the core of the absence-seizure prone phenotype of WAG/Rij rats, and that mGlu5 receptor enhancers are potential candidates to the treatment of absence epilepsy. PMID:22705340

Seeing left- or right-asymmetric tail wagging produces different emotional responses in dogs.

PubMed

Siniscalchi, Marcello; Lusito, Rita; Vallortigara, Giorgio; Quaranta, Angelo

2013-11-18

Left-right asymmetries in behavior associated with asymmetries in the brain are widespread in the animal kingdom, and the hypothesis has been put forward that they may be linked to animals' social behavior. Dogs show asymmetric tail-wagging responses to different emotive stimuli-the outcome of different activation of left and right brain structures controlling tail movements to the right and left side of the body. A crucial question, however, is whether or not dogs detect this asymmetry. Here we report that dogs looking at moving video images of conspecifics exhibiting prevalent left- or right-asymmetric tail wagging showed higher cardiac activity and higher scores of anxious behavior when observing left- rather than right-biased tail wagging. The finding that dogs are sensitive to the asymmetric tail expressions of other dogs supports the hypothesis of a link between brain asymmetry and social behavior and may prove useful to canine animal welfare theory and practice. Copyright © 2013 Elsevier Ltd. All rights reserved.

The Chromosomal Association of the Smc5/6 Complex Depends on Cohesion and Predicts the Level of Sister Chromatid Entanglement

PubMed Central

Jeppsson, Kristian; Carlborg, Kristian K.; Nakato, Ryuichiro; Berta, Davide G.; Lilienthal, Ingrid; Kanno, Takaharu; Lindqvist, Arne; Brink, Maartje C.; Dantuma, Nico P.; Katou, Yuki; Shirahige, Katsuhiko; Sjögren, Camilla

2014-01-01

The cohesin complex, which is essential for sister chromatid cohesion and chromosome segregation, also inhibits resolution of sister chromatid intertwinings (SCIs) by the topoisomerase Top2. The cohesin-related Smc5/6 complex (Smc5/6) instead accumulates on chromosomes after Top2 inactivation, known to lead to a buildup of unresolved SCIs. This suggests that cohesin can influence the chromosomal association of Smc5/6 via its role in SCI protection. Using high-resolution ChIP-sequencing, we show that the localization of budding yeast Smc5/6 to duplicated chromosomes indeed depends on sister chromatid cohesion in wild-type and top2-4 cells. Smc5/6 is found to be enriched at cohesin binding sites in the centromere-proximal regions in both cell types, but also along chromosome arms when replication has occurred under Top2-inhibiting conditions. Reactivation of Top2 after replication causes Smc5/6 to dissociate from chromosome arms, supporting the assumption that Smc5/6 associates with a Top2 substrate. It is also demonstrated that the amount of Smc5/6 on chromosomes positively correlates with the level of missegregation in top2-4, and that Smc5/6 promotes segregation of short chromosomes in the mutant. Altogether, this shows that the chromosomal localization of Smc5/6 predicts the presence of the chromatid segregation-inhibiting entities which accumulate in top2-4 mutated cells. These are most likely SCIs, and our results thus indicate that, at least when Top2 is inhibited, Smc5/6 facilitates their resolution. PMID:25329383

New outburst of GX 339-4 detected by Faulkes Telescope South

NASA Astrophysics Data System (ADS)

Russell, David M.; Lewis, Fraser; Gandhi, Poshak

2017-09-01

We have been monitoring the black hole X-ray binary GX 339-4 with the 2-m Faulkes Telescope South (at Siding Spring, Australia) since September 2007 (e.g. ATel #1586; #1962, #2459, #2547, #3191, #4162, Cadolle Bel et al. 2011).

[Behavior and functional state of the dopaminergic brain system in pups of depressive WAG/Rij rats].

PubMed

Malyshev, A V; Razumkina, E V; Rogozinskaia, É Ia; Sarkisova, K Iu; Dybynin, V A

2014-01-01

In the present work, it has been studied for the first time behavior and functional state of the dopaminergic brain system in pups of "depressive" WAG/Rij rats. Offspring of "depressive" WAG/Rij rats at age of 6-16 days compared with offspring of "normal" (non-depressed) outbred rats of the same age exhibited reduced rate of pshychomotor development, lower body weight, attenuation in integration of coordinated reflexes and vestibular function (greater latency of righting reflex, abnormal negative geotaxis), hyper-reactivity to tactile stimulation, reduced motivation to contact with mother (reduced infant-mother attachment). Differences in a nest seeking response induced by olfactory stimuli (olfactory discrimination test) and in locomotor activity (tests "gait reflex" and "small open field") have not been revealed. Acute injection of the antagonist of D2-like dopamine receptors clebopride 20 min before testing aggravated mother-oriented behavior in 15-days-old pups of both "depressive" and "non-depressive" rats. However this effect was greater in pups of "depressive" WAG/Rij rats compared with pups of "normal" rats that may indicate reduced functional activity of the dopaminergic brain system in offspring of "depressive" rats. It is proposed that reduced attachment behavior in pups of "depressive" WAG/Rij rats might be a consequence of maternal depression and associated with it reduced maternal care. Moreover, reduced attachment behavior in pups of "depressive" rats might be an early precursor (a marker) of depressive-like pathology which become apparent later in life (approximately at age of 3 months).

The optical counterpart of GX 339-4, a possible black hole X-ray source

NASA Technical Reports Server (NTRS)

Grindlay, J. E.

1979-01-01

Optical studies of the galactic X-ray source GX 339-4 (4U 1658-48), which led to its recent identification as reported by Doxsey et al. (1979), are presented. Reddening and distance estimates are given, as well as evidence for optical variability on differing time scales. The emission-line spectra and UBV photometry suggest that GX 339-4 may be at about 8 kpc and have a main-sequence B star binary companion. Both the optical spectrum and optical/X-ray luminosity ratio for GX 339-4 may be similar to Cir X-1.

Resolving complex chromosome structures during meiosis: versatile deployment of Smc5/6.

PubMed

Verver, Dideke E; Hwang, Grace H; Jordan, Philip W; Hamer, Geert

2016-03-01

The Smc5/6 complex, along with cohesin and condensin, is a member of the structural maintenance of chromosome (SMC) family, large ring-like protein complexes that are essential for chromatin structure and function. Thanks to numerous studies of the mitotic cell cycle, Smc5/6 has been implicated to have roles in homologous recombination, restart of stalled replication forks, maintenance of ribosomal DNA (rDNA) and heterochromatin, telomerase-independent telomere elongation, and regulation of chromosome topology. The nature of these functions implies that the Smc5/6 complex also contributes to the profound chromatin changes, including meiotic recombination, that characterize meiosis. Only recently, studies in diverse model organisms have focused on the potential meiotic roles of the Smc5/6 complex. Indeed, Smc5/6 appears to be essential for meiotic recombination. However, due to both the complexity of the process of meiosis and the versatility of the Smc5/6 complex, many additional meiotic functions have been described. In this review, we provide a clear overview of the multiple functions found so far for the Smc5/6 complex in meiosis. Additionally, we compare these meiotic functions with the known mitotic functions in an attempt to find a common denominator and thereby create clarity in the field of Smc5/6 research.

Broadband Spectroscopy Using Two Suzaku Observations of the HMXB GX 301-2

NASA Technical Reports Server (NTRS)

Suchy, Slawomir; Fuerst, Felix; Pottschmidt, Katja; Caballero, Isabel; Kreykenbohm, Ingo; Wilms, Joern; Markowitz, Alex; Rothschild, Richard E.

2012-01-01

We present the analysis of two Suzaku observations of GX 301-2 at two orbital phases after the periastron passage. Variations in the column density of the line-of-sight absorber are observed, consistent with accretion from a clumpy wind. In addition to a CRSF, multiple fluorescence emission lines were detected in both observations. The variations in the pulse profiles and the CRSF throughout the pulse phase have a signature of a magnetic dipole field. Using a simple dipole model we calculated the expected magnetic field values for different pulse phases and were able to extract a set of geometrical angles, loosely constraining the dipole geometry in the neutron star. From the variation of the CRSF width and energy, we found a geometrical solution for the dipole, making the inclination consistent with previously published values.

NuSTAR Observation of the Symbiotic System GX 1+4

NASA Astrophysics Data System (ADS)

Wolff, Michael Thomas; Becker, Peter A.; Enoto, Teruaki; Pottschmidt, Katja; Wood, Kent

2017-08-01

We report on a NuSTAR observation of the symbiotic binary system GX 1+4. GX 1+4 is one of a small number of systems with a red giant mass donor and a magnetic neutron star in orbit around each other. The accreting pulsar in GX 1+4 has a spin period of ~150 seconds with epochs of both spin-up and spin-down. The orbital period that has not been determined. Magnetic accretion theory in such systems suggests that the neutron star has a magnetic field in the range 1013-1014 Gauss although this is not settled because no cyclotron absorption feature has been observed in the X-ray spectrum. The NuSTAR spectrum shows broad Fe-line emission near ~6.5 keV and also shows a broad power law shape detected up to ~60 keV. We analyze and discuss the NuSTAR X-ray data with particular attention to the question of what can the spectrum tell us about the structure of the accretion flow onto the neutron star and the magnetic field strength.

[Effects of high +Gx during simulated spaceship emergency return on learning and memory in rats].

PubMed

Xu, Zhi-peng; Sun, Xi-qing; Liu, Ting-song; Wu, Bin; Zhang, Shu; Wu, Ping

2005-02-01

To observe the effects of high +Gx during simulated spaceship emergency return on learning and memory in rats. Thirty two male SD rats were randomly divided into control group, 7 d simulated weightlessness group, +15 Gx/180 s group and +15 Gx/180 s exposure after 7 d simulated weightlessness group, with 8 rats in each group. The changes of learning and memory in rats were measured after stresses by means of Y-maze test and step-through test. In Y-maze test, as compared with control group, percentage of correct reactions decreased significantly (P<0.01) and reaction time increased significantly (P<0.01) in hypergravity after simulated weightlessness group at all time after stress; as compared with +15 Gx group or simulated weightlessness group, percentage of correct reactions decreased significantly (P< 0.05) and reaction time increased significantly (P< 0.05) immediately after stress. In step-through test, as compared with control group, total time increased significantly (P<0.01) in hypergravity after simulated weightlessness group at 1 d after stress; latent time decreased significantly (P<0.01) and number of errors increased significantly (P< 0.01) at all the time after stress. As compared with +15 Gx group, total time increased significantly (P<0.05) immediately, 1 d after stress. As compared with simulated weightlessness group, total time and number of errors increased significantly (P<0.05) immediately after stress. It is suggested that +15 Gx/180 s and simulated weightlessness may affect the ability of learning and memory of rats. Simulated weightlessness for 7 d can aggravate the effect of +Gx on learning and memory ability in rats.

Seismic Tremors and Three-Dimensional Magma Wagging

NASA Astrophysics Data System (ADS)

Liao, Y.; Bercovici, D.

2015-12-01

Seismic tremor is a feature shared by many silicic volcanoes and is a precursor of volcanic eruption. Many of the characteristics of tremors, including their frequency band from 0.5 Hz to 7 Hz, are common for volcanoes with very different geophysical and geochemical properties. The ubiquitous characteristics of tremor imply that it results from some generation mechanism that is common to all volcanoes, instead of being unique to each volcano. Here we present new analysis on the magma-wagging mechanism that has been proposed to generate tremor. The model is based on the suggestion given by previous work (Jellinek & Bercovici 2011; Bercovici et.al. 2013) that the magma column is surrounded by a compressible, bubble-rich foam annulus while rising inside the volcanic conduit, and that the lateral oscillation of the magma inside the annulus causes observable tremor. Unlike the previous two-dimensional wagging model where the displacement of the magma column is restricted to one vertical plane, the three-dimensional model we employ allows the magma column to bend in different directions and has angular motion as well. Our preliminary results show that, without damping from viscous deformation of the magma column, the system retains angular momentum and develops elliptical motion (i.e., the horizontal displacement traces an ellipse). In this ''inviscid'' limit, the magma column can also develop instabilities with higher frequencies than what is found in the original two-dimensional model. Lateral motion can also be out of phase for various depths in the magma column leading to a coiled wagging motion. For the viscous-magma model, we predict a similar damping rate for the uncoiled magma column as in the two-dimensional model, and faster damping for the coiled magma column. The higher damping thus requires the existence of a forcing mechanism to sustain the oscillation, for example the gas-driven Bernoulli effect proposed by Bercovici et al (2013). Finally, using our new 3

An X-ray Investigation of the NGC 346 Field in the SMC (2): The Field Population

NASA Technical Reports Server (NTRS)

Naze, Y.; Hartwell, J. M.; Stevens, I. R.; Manfroid, J.; Marchenko. S.; Corcoran, M. F.; Moffat, A. F. J.; Skalkowski, G.; White, Nicholas E. (Technical Monitor)

2002-01-01

We present results from a Chandra observation of the NGC 346 cluster, the ionizing source of N66, the most luminous H II region and the largest star formation region in the SMC. In the first part of this investigation, we have analysed the X-ray properties of the cluster itself and the remarkable star HD 5980. But the field contains additional objects of interest. In total, 79 X-ray point sources were detected in the Chandra observation and we investigate here their characteristics in details. The sources possess rather high HRs, and their cumulative luminosity function is steeper than the SMC's trend. Their absorption columns suggest that most of the sources belong to NGC 346. Using new UBVRI imaging with the ESO 2.2m telescope, we also discovered possible counterparts for 36 of these X-ray sources. Finally, some objects show X-ray and/or optical variability, and thus need further monitoring.

SMC Message Browser Projects

NASA Technical Reports Server (NTRS)

Wichmann, Benjamin C.

2013-01-01

I work directly with the System Monitoring and Control (SMC) software engineers who develop, test and release custom and commercial software in support of the Kennedy Space Center Spaceport Command and Control System. (SCCS). SMC uses Commercial Off-The-Shelf (COTS) Enterprise Management Systems (EMS) software which provides a centralized subsystem for configuring, monitoring, and controlling SCCS hardware and software used in the Control Rooms. There are multiple projects being worked on using the COTS EMS software. I am currently working with the HP Operations Manager for UNIX (OMU) software which allows Master Console Operators (MCO) to access, view and interpret messages regarding the status of the SCCS hardware and software. The OMU message browser gets cluttered with messages which can make it difficult for the MCO to manage. My main project involves determining ways to reduce the number of messages being displayed in the OMU message browser. I plan to accomplish this task in two different ways: (1) by correlating multiple messages into one single message being displayed and (2) to create policies that will determine the significance of each message and whether or not it needs to be displayed to the MCO. The core idea is to lessen the number of messages being sent to the OMU message browser so the MCO can more effectively use it.

GX 339-4 Approaching Quiescence ?

NASA Astrophysics Data System (ADS)

Lewis, F.; Russell, D. M.

2009-10-01

The black hole X-ray binary (BHXB) GX 339-4 continues to fade in the optical, and has now reached its faintest optical flux since (at least) the start of its 2006-7 outburst (ATels #968, #1027). In this outburst, the source reached peak magnitude values of V ~ 15 and I ~ 14. On the decline of the outburst the source twice re-brightened for several months and displayed large amplitude variability (ATels #1586, #1588, #1945, #1954, #1960, #1962).

Smc1β is required for activation of SAC during mouse oocyte meiosis.

PubMed

Miao, Yilong; Zhou, Changyin; Cui, Zhaokang; Dai, Xiaoxin; Zhang, Mianqun; Lu, Yajuan; Xiong, Bo

2017-03-19

Smc1β is a meiosis-specific cohesin subunit that is essential for sister chromatid cohesion and DNA recombination. Previous studies have shown that Smc1β-deficient mice in both sexes are sterile. Ablation of Smc1β during male meiosis leads to the blockage of spermatogenesis in pachytene stage, and ablation of Smc1β during female meiosis generates a highly error-prone oocyte although it could develop to metaphase II stage. However, the underlying mechanisms regarding how Smc1β maintains the correct meiotic progression in mouse oocytes have not been clearly defined. Here, we find that GFP-fused Smc1β is expressed and localized to the chromosomes from GV to MII stages during mouse oocyte meiotic maturation. Knockdown of Smc1β by microinjection of gene-specific morpholino causes the impaired spindle apparatus and chromosome alignment which are highly correlated with the defective kinetochore-microtubule attachments, consequently resulting in a prominently higher incidence of aneuploid eggs. In addition, the premature extrusion of polar bodies and escape of metaphase I arrest induced by low dose of nocodazole treatment in Smc1β-depleted oocytes indicates that Smc1β is essential for activation of spindle assembly checkpoint (SAC) activity. Collectively, we identify a novel function of Smc1β as a SAC participant beyond its role in chromosome cohesion during mouse oocyte meiosis.

Broadband Spectroscopy Using Two Suzaku Observations of the HMXB GX 301-2

NASA Astrophysics Data System (ADS)

Suchy, Slawomir; Fürst, Felix; Pottschmidt, Katja; Caballero, Isabel; Kreykenbohm, Ingo; Wilms, Jörn; Markowitz, Alex; Rothschild, Richard E.

2012-02-01

We present the analysis of two Suzaku observations of GX 301-2 at two orbital phases after the periastron passage. Variations in the column density of the line-of-sight absorber are observed, consistent with accretion from a clumpy wind. In addition to a cyclotron resonance scattering feature (CRSF), multiple fluorescence emission lines were detected in both observations. The variations in the pulse profiles and the CRSF throughout the pulse phase have a signature of a magnetic dipole field. Using a simple dipole model we calculated the expected magnetic field values for different pulse phases and were able to extract a set of geometrical angles, loosely constraining the dipole geometry in the neutron star. From the variation of the CRSF width and energy, we found a geometrical solution for the dipole, making the inclination consistent with previously published values.

A search for optical pulsations from GX 1+4 at H-alpha

NASA Technical Reports Server (NTRS)

Krzeminski, W.; Priedhorsky, W. C.

1978-01-01

H-alpha observations of the binary-star candidate for the slowly pulsating hard X-ray source GX 1+4 are reported which were undertaken to search for pulsations in the H-alpha flux that are synchronous with the X-ray period of about 2 min. No significant periodic variation of the candidate star was detected in the frequency band searched. Three-sigma upper limits of 1.7% (sinusoidal pulse shape) and 0.7% (X-ray pulse shape) are set for the pulsed fraction of the H-alpha flux. It is noted that because of possible diffusion from a cloud that is optically thick to Balmer radiation, the observed lack of pulsations in the H-alpha flux need not compromise the identification of GX 1+4 with the candidate star.

SMC1A recruits tumor-associated-fibroblasts (TAFs) and promotes colorectal cancer metastasis.

PubMed

Zhou, Pengyang; Xiao, Nan; Wang, Jian; Wang, Zhanhuai; Zheng, Shuchun; Shan, Siyang; Wang, Jianping; Du, Jinlin; Wang, Jianwei

2017-01-28

Tumor-associated-fibroblasts (TAFs) are the most important host cells in the stroma and take part in extracellular matrix construction and cancer colony development. During cancer colonization, seed cells from primary tumor can reconstruct the microenvironment by recruiting circulating cancer cells and TAFs to the metastasis site. Previous studies have established that SMC1A, a subunit of cohesin, is an important trigger signal for liver metastasis in colorectal cancer. We investigated the particular effects as well as the underlying mechanism of SMC1A on TAFs recruitment during liver metastasis of colorectal cancer. Here, We found that: first, the high expression of SMC1A in colorectal cancer cells promotes the invasiveness and the viability of these cells by recruiting circulating TAFs, facilitating early tumor construction and tumorigenesis; second, different expression levels of SMC1A influenced the reformation of fibroblasts, which assisted tumorigenesis, and third, expression of SMC1A stimulated the secretion of the inflammatory mediators of TNF-α and IL-1β, and up-regulated the transcriptional expression of MMP2 and VEGF-β, both of which were involved in the tumor-related gene pathway. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Maternal care affects EEG properties of spike-wave seizures (including pre- and post ictal periods) in adult WAG/Rij rats with genetic predisposition to absence epilepsy.

PubMed

Sitnikova, Evgenia; Rutskova, Elizaveta M; Raevsky, Vladimir V

2016-10-01

WAG/Rij rats have a genetic predisposition to absence epilepsy and develop spontaneous spike-wave discharges in EEG during late ontogenesis (SWD, EEG manifestation of absence epilepsy). Changes in an environment during early postnatal ontogenesis can influence the genetically predetermined absence epilepsy. Here we examined the effect of maternal environment during weaning period on the EEG manifestation of absence epilepsy in adulthood. Experiments were performed in the offspring of WAG/Rij and Wistar rats. The newborn pups were fostered to dams of the same (in-fostering) or another strain (cross-fostering). Age-matched control WAG/Rij and Wistar rats were reared by their biological mothers. Absence seizures were uncommon in Wistar and were not aggravated in both in- and cross-fostered groups. In WAG/Rij rats, fewer SWD were found in the cross-fostered as compared to the in-fostered group. The cross-fostered WAG/Rij rats showed higher percentage of short-lasting SWD with duration <2s. The mean frequency of EEG at the beginning of SWD in the cross-fostered WAG/Rij rats was lower than in control (8.82 vs 9.25Hz), but it was higher in a period of 1.5s before and after SWD. It was concluded that a healthier maternal environment is able to alleviate genetically predetermined absence seizures in adulthood through changes in EEG rhythmic activity. Copyright © 2016 Elsevier Inc. All rights reserved.

Low-metallicity (sub-SMC) massive stars

NASA Astrophysics Data System (ADS)

Garcia, Miriam; Herrero, Artemio; Najarro, Francisco; Camacho, Inés; Lennon, Daniel J.; Urbaneja, Miguel A.; Castro, Norberto

2017-11-01

The double distance and metallicity frontier marked by the SMC has been finally broken with the aid of powerful multi-object spectrographs installed at 8-10m class telescopes. VLT, GTC and Keck have enabled studies of massive stars in dwarf irregular galaxies of the Local Group with poorer metal-content than the SMC. The community is working to test the predictions of evolutionary models in the low-metallicity regime, set the new standard for the metal-poor high-redshift Universe, and test the extrapolation of the physics of massive stars to environments of decreasing metallicity. In this paper, we review current knowledge on this topic.

BROADBAND SPECTROSCOPY USING TWO SUZAKU OBSERVATIONS OF THE HMXB GX 301-2

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suchy, Slawomir; Markowitz, Alex; Rothschild, Richard E.

2012-02-01

We present the analysis of two Suzaku observations of GX 301-2 at two orbital phases after the periastron passage. Variations in the column density of the line-of-sight absorber are observed, consistent with accretion from a clumpy wind. In addition to a cyclotron resonance scattering feature (CRSF), multiple fluorescence emission lines were detected in both observations. The variations in the pulse profiles and the CRSF throughout the pulse phase have a signature of a magnetic dipole field. Using a simple dipole model we calculated the expected magnetic field values for different pulse phases and were able to extract a set ofmore » geometrical angles, loosely constraining the dipole geometry in the neutron star. From the variation of the CRSF width and energy, we found a geometrical solution for the dipole, making the inclination consistent with previously published values.« less

Temperature-dependent thermal and thermoelectric properties of n -type and p -type S c1 -xM gxN

NASA Astrophysics Data System (ADS)

Saha, Bivas; Perez-Taborda, Jaime Andres; Bahk, Je-Hyeong; Koh, Yee Rui; Shakouri, Ali; Martin-Gonzalez, Marisol; Sands, Timothy D.

2018-02-01

Scandium Nitride (ScN) is an emerging rocksalt semiconductor with octahedral coordination and an indirect bandgap. ScN has attracted significant attention in recent years for its potential thermoelectric applications, as a component material in epitaxial metal/semiconductor superlattices, and as a substrate for defect-free GaN growth. Sputter-deposited ScN thin films are highly degenerate n -type semiconductors and exhibit a large thermoelectric power factor of ˜3.5 ×10-3W /m -K2 at 600-800 K. Since practical thermoelectric devices require both n- and p-type materials with high thermoelectric figures-of-merit, development and demonstration of highly efficient p-type ScN is extremely important. Recently, the authors have demonstrated p-type S c1 -xM gxN thin film alloys with low M gxNy mole-fractions within the ScN matrix. In this article, we demonstrate temperature dependent thermal and thermoelectric transport properties, including large thermoelectric power factors in both n- and p-type S c1 -xM gxN thin film alloys at high temperatures (up to 850 K). Employing a combination of temperature-dependent Seebeck coefficient, electrical conductivity, and thermal conductivity measurements, as well as detailed Boltzmann transport-based modeling analyses of the transport properties, we demonstrate that p-type S c1 -xM gxN thin film alloys exhibit a maximum thermoelectric power factor of ˜0.8 ×10-3W /m -K2 at 850 K. The thermoelectric properties are tunable by adjusting the M gxNy mole-fraction inside the ScN matrix, thereby shifting the Fermi energy in the alloy films from inside the conduction band in case of undoped n -type ScN to inside the valence band in highly hole-doped p -type S c1 -xM gxN thin film alloys. The thermal conductivities of both the n- and p-type films were found to be undesirably large for thermoelectric applications. Thus, future work should address strategies to reduce the thermal conductivity of S c1 -xM gxN thin-film alloys, without affecting

Decreased ERp57 Expression in WAG/Rij Rats Thalamus and Cortex; Possible Correlation with Absence Epilepsy.

PubMed

Sahin, Deniz; Karadenizli, Sabriye; Kasap, Murat; Oztas, Berrin; Kir, Hale Maral; Akpinar, Gurler; Ates, Nurbay

2018-02-06

The role of intracellular proteins in the pathogenesis of absence epilepsy were mentioned. These proteins are thought to be related to energy generation, signal transduction, inflammation processes and membrane conductance. The investigation of protein profile of the genetically epileptic rat brains was the main subject of this study. For this, a 2D-gel electrophoresis based comparative proteome analysis was performed using thalamus tissue of genetic absence epileptic WAG/Rij and age matched Wistar rats. Regulated spots displaying differences in their abundance were identified using MALDI-TOF/TOF. Among the six spots (DHRS9, BR44, HINT1, CREM, SPRE and PDIA3/ERp57) the highest mascot score was attributed to ERp57 a neuroprotective/neurodegenerative system associated protein. Western Blot analyses were performed to validate changes occurring at ERp57 in thalamus and also identify changes in fronto-parietal cortex. Reductions in the expression levels of ERp57 were detected in the thalamic and the fronto-parietal brain regions of the WAG/Rij rats in comparison to Wistar rats. Such difference might be associated with the pathogenic mechanisms dictating the absence epilepsy. Lower levels of ERp57 may be playing an important role in the development of spontaneous seizures activity seen in the absence epileptic WAG/Rij rats strain. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Meiotic Cohesin SMC1β Provides Prophase I Centromeric Cohesion and Is Required for Multiple Synapsis-Associated Functions

PubMed Central

Biswas, Uddipta; Wetzker, Cornelia; Lange, Julian; Christodoulou, Eleni G.; Seifert, Michael; Beyer, Andreas; Jessberger, Rolf

2013-01-01

Cohesin subunit SMC1β is specific and essential for meiosis. Previous studies showed functions of SMC1β in determining the axis-loop structure of synaptonemal complexes (SCs), in providing sister chromatid cohesion (SCC) in metaphase I and thereafter, in protecting telomere structure, and in synapsis. However, several central questions remained unanswered and concern roles of SMC1β in SCC and synapsis and processes related to these two processes. Here we show that SMC1β substantially supports prophase I SCC at centromeres but not along chromosome arms. Arm cohesion and some of centromeric cohesion in prophase I are provided by non-phosphorylated SMC1α. Besides supporting synapsis of autosomes, SMC1β is also required for synapsis and silencing of sex chromosomes. In absence of SMC1β, the silencing factor γH2AX remains associated with asynapsed autosomes and fails to localize to sex chromosomes. Microarray expression studies revealed up-regulated sex chromosome genes and many down-regulated autosomal genes. SMC1β is further required for non-homologous chromosome associations observed in absence of SPO11 and thus of programmed double-strand breaks. These breaks are properly generated in Smc1β−/− spermatocytes, but their repair is delayed on asynapsed chromosomes. SMC1α alone cannot support non-homologous associations. Together with previous knowledge, three main functions of SMC1β have emerged, which have multiple consequences for spermatocyte biology: generation of the loop-axis architecture of SCs, homologous and non-homologous synapsis, and SCC starting in early prophase I. PMID:24385917

Interlaminar fracture of random short-fiber SMC composite

NASA Technical Reports Server (NTRS)

Wang, S. S.; Suemasu, H.; Zahlan, N. M.

1984-01-01

In the experimental phase of the present study of the interlaminar fracture behavior of a randomly oriented short fiber sheet molding compound (SMC) composite, the double cantilever beam fracture test is used to evaluate the mode I interlaminar fracture toughness of different composite thicknesses. In the analytical phase of this work, a geometrically nonlinear analysis is introduced in order to account for large deflections and nonlinear load deflection curves in the evaluation of interlaminar fracture toughness. For the SMC-R50 material studied, interlaminar toughness is an order of magnitude higher than that of unreinforced neat resin, due to unusual damage mechanisms ahead of the crack tip, together with significant fiber bridging across crack surfaces. Composite thickness effects on interlaminar fracture are noted to be appreciable, and a detailed discussion is given on the influence of SMC microstructure.

Temporal and Embryonic Lineage-Dependent Regulation of Human Vascular SMC Development by NOTCH3

PubMed Central

Granata, Alessandra; Bernard, William G.; Zhao, Ning; Mccafferty, John; Lilly, Brenda

2015-01-01

Vascular smooth muscle cells (SMCs), which arise from multiple embryonic progenitors, have unique lineage-specific properties and this diversity may contribute to spatial patterns of vascular diseases. We developed in vitro methods to generate distinct vascular SMC subtypes from human pluripotent stem cells, allowing us to explore their intrinsic differences and the mechanisms involved in SMC development. Since Notch signaling is thought to be one of the several key regulators of SMC differentiation and function, we profiled the expression of Notch receptors, ligands, and downstream elements during the development of origin-specific SMC subtypes. NOTCH3 expression in our in vitro model varied in a lineage- and developmental stage-specific manner so that the highest expression in mature SMCs was in those derived from paraxial mesoderm (PM). This pattern was consistent with the high expression level of NOTCH3 observed in the 8–9 week human fetal descending aorta, which is populated by SMCs of PM origin. Silencing NOTCH3 in mature SMCs in vitro reduced SMC markers in cells of PM origin preferentially. Conversely, during early development, NOTCH3 was highly expressed in vitro in SMCs of neuroectoderm (NE) origin. Inhibition of NOTCH3 in early development resulted in a significant downregulation of specific SMC markers exclusively in the NE lineage. Corresponding to this prediction, the Notch3-null mouse showed reduced expression of Acta2 in the neural crest-derived SMCs of the aortic arch. Thus, Notch3 signaling emerges as one of the key regulators of vascular SMC differentiation and maturation in vitro and in vivo in a lineage- and temporal-dependent manner. PMID:25539150

Lightweight sheet molding compound (SMC) composites containing cellulose nanocrystals

Treesearch

Amir Asadi; Mark Miller; Arjun V. Singh; Robert J. Moon; Kyriaki Kalaitzidou

2017-01-01

A scalable technique was introduced to produce high volume lightweight composites using sheet molding compound (SMC) manufacturing method by replacing 10 wt% glass fibers (GF) with a small amount of cellulose nanocrystals (CNC). The incorporation of 1 and 1.5 wt% CNC by dispersing in the epoxy matrix of short GF/epoxy SMC composites with 25 wt% GF content (25GF/CNC-...

Hard X-ray imaging of the Galactic black hole candidate GX 339 - 4

NASA Technical Reports Server (NTRS)

Covault, C. E.; Grindlay, J. E.; Manandhar, R. P.

1992-01-01

Imaging and spectral observations in the energy range 25-250 keV of the black hole candidate GX 339 - 4 have been obtained with the Energetic X-ray Imaging Telescope Experiment. Observations were made during a balloon flight from Alice Springs, Australia on UT 1989 May 8-10. A single source of nearly 6-sigma significance is detected near the center of the 3.4-deg field of view with a position consistent with GX 339 - 4. This is the first imaging observation of GX 339 - 4 at hard X-ray energies. This result confirms previously reported results from nonimaging experiments showing significant hard X-ray flux up to greater than about 60 keV, with a power-law spectral fit similar to the other black hole candidates such as Cygnus X - 1. The source may have been in an outburst state similar to that recently detected with BATSE on GRO.

Low Luminosity States of the Black Hole Candidate GX 339-4. 2; Timing Analysis

NASA Technical Reports Server (NTRS)

Nowak, Michael A.; Wilms, Joern; Dove, James B.

1999-01-01

Here we present timing analysis of a set of eight Rossi X-ray Timing Explorer (RXTE) observations of the black hole candidate GX 339-4 that were taken during its hard/low state. On long time scales, the RXTE All Sky Monitor data reveal evidence of a 240 day periodicity, comparable to timescales expected from warped, precessing accretion disks. On short timescales all observations save one show evidence of a persistent f(qpo approximately equals 0.3 Hz quasi-periodic oscillations (QPO)). The broad band (10 (exp -3) to 10 (exp2) Hz) power appears to be dominated by two independent processes that can be modeled as very broad Lorentzians with Q approximately less than - 1. The coherence function between soft and hard photon variability shows that if these are truly independent processes, then they are individually coherent, but they are incoherent with one another. This is evidenced by the fact that the coherence function between the hard and soft variability is near unity between 5 x 10 (exp -3) but shows evidence of a dip at f approximately equals 1 Hz. This is the region of overlap between the broad Lorentzian fits to the Power Spectral Density (PSD). Similar to Cyg X-1, the coherence also drops dramatically at frequencies approximately greater than 1O Hz. Also similar to Cyg X-1, the hard photon variability is seen to lag the soft photon variability with the lag time increasing with decreasing Fourier frequency. The magnitude of this time lag appears to be positively correlated with the flux of GX 339-4. We discuss all of these observations in light of current theoretical models of both black hole spectra and temporal variability.

Variability of the symbiotic X-ray binary GX 1+4. Enhanced activity near periastron passage

NASA Astrophysics Data System (ADS)

Iłkiewicz, Krystian; Mikołajewska, Joanna; Monard, Berto

2017-05-01

Context. GX 1+4 belongs to a rare class of X-ray binaries with red giant donors, symbiotic X-ray binaries. It has a history of complicated variability on multiple timescales in the optical light and X-rays. The nature of this variability remains poorly understood. Aims: We aim to study variability of GX 1+4 on long timescale in X-ray and optical bands. Methods: We took X-ray observations from the INTEGRAL Soft Gamma-Ray Imager and RXTE All Sky Monitor. Optical observations were made with the INTEGRAL Optical Monitoring Camera. Results: The variability of GX 1+4 both in optical light and hard X-ray emission (>17 keV) is dominated by 50-70 d quasi-periodic changes. The amplitude of this variability is highest during the periastron passage, while during the potential neutron star eclipse the system is always at minimum. This confirms the 1161 d orbital period that has had been proposed for the system based on radial velocity curve. Neither the quasi-periodic variability or the orbital period are detected in soft X-ray emission (1.3-12.2 keV), where the binary shows no apparent periodicity.

Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression.

PubMed

Pryzhkova, Marina V; Jordan, Philip W

2016-04-15

Correct duplication of stem cell genetic material and its appropriate segregation into daughter cells are requisites for tissue, organ and organism homeostasis. Disruption of stem cell genomic integrity can lead to developmental abnormalities and cancer. Roles of the Smc5/6 structural maintenance of chromosomes complex in pluripotent stem cell genome maintenance have not been investigated, despite its important roles in DNA synthesis, DNA repair and chromosome segregation as evaluated in other model systems. Using mouse embryonic stem cells (mESCs) with a conditional knockout allele of Smc5, we showed that Smc5 protein depletion resulted in destabilization of the Smc5/6 complex, accumulation of cells in G2 phase of the cell cycle and apoptosis. Detailed assessment of mitotic mESCs revealed abnormal condensin distribution and perturbed chromosome segregation, accompanied by irregular spindle morphology, lagging chromosomes and DNA bridges. Mutation of Smc5 resulted in retention of Aurora B kinase and enrichment of condensin on chromosome arms. Furthermore, we observed reduced levels of Polo-like kinase 1 at kinetochores during mitosis. Our study reveals crucial requirements of the Smc5/6 complex during cell cycle progression and for stem cell genome maintenance. © 2016. Published by The Company of Biologists Ltd.

Discovery of the binary nature of SMC X-1 from Uhuru.

NASA Technical Reports Server (NTRS)

Schreier, E.; Giacconi, R.; Gursky, H.; Kellogg, E.; Tananbaum, H.

1972-01-01

The X-ray source in the Small Magellanic Cloud SMC X-1 was observed by Uhuru on numerous occasions from December 1970 through April 1972. As previously reported by Leong et al. (1971), the source was seen to be variable. It was found that SMC X-1 occults with a period of 3.8927 days. The energy spectrum is cut off at low energies and flat. There is no large-amplitude periodic pulsation. The luminosity observed makes the binary source SMC X-1 comparable in strength to both the stronger galactic sources and the discrete sources in the Large Magellanic Cloud.

Discovery of orbital decay in SMC X-1

NASA Technical Reports Server (NTRS)

Levine, A.; Rappaport, S.; Boynton, P.; Deeter, J.; Nagase, F.

1992-01-01

The results are reported of three observations of the binary X ray pulsar SMC X-1 with the Ginga satellite. Timing analyses of the 0.71 s X ray pulsations yield Doppler delay curves which, in turn, provide the most accurate determination of the SMC X-1 orbital parameters available to date. The orbital phase of the 3.9 day orbit is determined in May 1987, Aug. 1988, and Aug. 1988 with accuracies of 11, 1, and 3.5 s, respectively. These phases are combined with two previous determinations of the orbital phase to yield the rate of change in the orbital period: P sub orb/P sub orb = (-3.34 + or - 0.023) x 10(exp -6)/yr. An interpretation of this measurement and the known decay rate for the orbit of Cen X-3 is made in the context of tidal evolution. Finally, a discussion is presented of the relation among the stellar evolution, orbital decay, and neutron star spinup time scales for the SMC X-1 system.

Combing Chromosomal DNA Mediated by the SMC Complex: Structure and Mechanisms.

PubMed

Kamada, Katsuhiko; Barillà, Daniela

2018-02-01

Genome maintenance requires various nucleoid-associated factors in prokaryotes. Among them, the SMC (Structural Maintenance of Chromosomes) protein has been thought to play a static role in the organization and segregation of the chromosome during cell division. However, recent studies have shown that the bacterial SMC is required to align left and right arms of the emerging chromosome and that the protein dynamically travels from origin to Ter region. A rod form of the SMC complex mediates DNA bridging and has been recognized as a machinery responsible for DNA loop extrusion, like eukaryotic condensin or cohesin complexes, which act as chromosome organizers. Attention is now turning to how the prototype of the complex is loaded on the entry site and translocated on chromosomal DNA, explaining its overall conformational changes at atomic levels. Here, we review and highlight recent findings concerning the prokaryotic SMC complex and discuss possible mechanisms from the viewpoint of protein architecture. © 2017 The Authors. BioEssays Published by WILEY Periodicals, Inc.

An X-ray Investigation of the NGC 346 Field in the SMC (2): The Field Population

NASA Technical Reports Server (NTRS)

Naze, Y.; Hartwell, J. M.; Stevens, I. R.; Manfroid, J.; Marchenko, S.; Corcoran, M. F.; Moffat, A. F. J.; Skalkowski, G.

2003-01-01

We present results from a Chandra observation of the NGC 346 cluster, which is the ionizing source of N66, the most luminous HII region and the largest star formation region in the SMC. In the first part of this investigation, we have analysed the X-ray properties of the cluster itself and the remarkable star HD 5980. But the field contains additional objects of interest. In total, 79 X-ray point sources were detected in the Chandra observation: this is more than five times the number of sources detected by previous X-ray surveys. We investigate here their characteristics in detail. The sources possess rather high hardness ratios, and their cumulative luminosity function is steeper than that for the rest of the SMC at higher .luminosities. Their absorption columns suggest that most of the sources belong to NGC346. Using new UBV RI imaging with the ESO 2.2m telescope, we also discovered possible counterparts for 36 of these X-ray sources and estimated a B spectral type for a large number of these counterparts. This tends to suggest that most of the X-ray sources in the field are in fact X-ray binaries. Finally, some objects show X-ray and/or optical variability, with a need for further monitoring.

CHANDRA AND SWIFT X-RAY OBSERVATIONS OF THE X-RAY PULSAR SMC X-2 DURING THE OUTBURST OF 2015

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, K. L.; Hu, C.-P; Lin, L. C. C.

2016-09-10

We report the Chandra /HRC-S and Swift /XRT observations for the 2015 outburst of the high-mass X-ray binary pulsar in the Small Magellanic Cloud, SMC X-2. While previous studies suggested that either an O star or a Be star in the field is the high-mass companion of SMC X-2, our Chandra /HRC-S image unambiguously confirms the O-type star as the true optical counterpart. Using the Swift /XRT observations, we extracted accurate orbital parameters of the pulsar binary through a time of arrivals analysis. In addition, there were two X-ray dips near the inferior conjunction, which are possibly caused by eclipsesmore » or an ionized high-density shadow wind near the companion’s surface. Finally, we propose that an outflow driven by the radiation pressure from day ∼10 played an important role in the X-ray/optical evolution of the outburst.« less

Absorption and scattering by interstellar dust in the silicon K-edge of GX 5-1

NASA Astrophysics Data System (ADS)

Zeegers, S. T.; Costantini, E.; de Vries, C. P.; Tielens, A. G. G. M.; Chihara, H.; de Groot, F.; Mutschke, H.; Waters, L. B. F. M.; Zeidler, S.

2017-03-01

Context. We study the absorption and scattering of X-ray radiation by interstellar dust particles, which allows us to access the physical and chemical properties of dust. The interstellar dust composition is not well understood, especially on the densest sight lines of the Galactic plane. X-rays provide a powerful tool in this study. Aims: We present newly acquired laboratory measurements of silicate compounds taken at the Soleil synchrotron facility in Paris using the Lucia beamline. The dust absorption profiles resulting from this campaign were used in this pilot study to model the absorption by interstellar dust along the line of sight of the low-mass X-ray binary GX 5-1. Methods: The measured laboratory cross-sections were adapted for astrophysical data analysis and the resulting extinction profiles of the Si K-edge were implemented in the SPEX spectral fitting program. We derive the properties of the interstellar dust along the line of sight by fitting the Si K-edge seen in absorption in the spectrum of GX 5-1. Results: We measured the hydrogen column density towards GX 5-1 to be 3.40 ± 0.1 × 1022 cm-2. The best fit of the silicon edge in the spectrum of GX 5-1 is obtained by a mixture of olivine and pyroxene. In this study, our modeling is limited to Si absorption by silicates with different Mg:Fe ratios. We obtained an abundance of silicon in dust of 4.0 ± 0.3 × 10-5 per H atom and a lower limit for total abundance, considering both gas and dust of >4.4 × 10-5 per H atom, which leads to a gas to dust ratio of >0.22. Furthermore, an enhanced scattering feature in the Si K-edge may suggest the presence of large particles along the line of sight.

Seismic tremors and magma wagging during explosive volcanism.

PubMed

Jellinek, A Mark; Bercovici, David

2011-02-24

Volcanic tremor is a ubiquitous feature of explosive eruptions. This oscillation persists for minutes to weeks and is characterized by a remarkably narrow band of frequencies from about 0.5 Hz to 7 Hz (refs 1-4). Before major eruptions, tremor can occur in concert with increased gas flux and related ground deformation. Volcanic tremor is thus of particular value for eruption forecasting. Most models for volcanic tremor rely on specific properties of the geometry, structure and constitution of volcanic conduits as well as the gas content of the erupting magma. Because neither the initial structure nor the evolution of the magma-conduit system will be the same from one volcano to the next, it is surprising that tremor characteristics are so consistent among different volcanoes. Indeed, this universality of tremor properties remains a major enigma. Here we employ the contemporary view that silicic magma rises in the conduit as a columnar plug surrounded by a highly vesicular annulus of sheared bubbles. We demonstrate that, for most geologically relevant conditions, the magma column will oscillate or 'wag' against the restoring 'gas-spring' force of the annulus at observed tremor frequencies. In contrast to previous models, the magma-wagging oscillation is relatively insensitive to the conduit structure and geometry, which explains the narrow band of tremor frequencies observed around the world. Moreover, the model predicts that as an eruption proceeds there will be an upward drift in both the maximum frequency and the total signal frequency bandwidth, the nature of which depends on the explosivity of the eruption, as is often observed.

Hard X-ray Emission along the Z Track in GX 17 + 2

NASA Astrophysics Data System (ADS)

Ding, G. Q.; Huang, C. P.

2015-09-01

Using the data from the Proportional Counter Array (PCA) and the High-Energy X-ray Timing Experiment (HEXTE) on board Rossi X-Ray Timing Explorer for Z source GX 17 + 2, we investigate the evolution of its PCA spectra and HEXTE spectra along a `Z' track on its hardness-intensity diagram. A hard X-ray tail is detected in the HEXTE spectra. The detected hard X-ray tails are discontinuously scattered throughout the Z track. The found hard X-ray tail hardens from the horizontal branch, through the normal branch, to the flaring branch in principle and it contributes ˜(20-50)% of the total flux in 20-200 keV. Our joint fitting results of the PCA + HEXTE spectra in 3-200 keV show that the portion of Comptonization in the Bulk-Motion Comptonization (BMC) model accounts for the hard X-ray tail, which indicates that the BMC process could be responsible for the detected hard tail. The temperature of the seed photons for BMC is ˜2.7 keV, implying that these seed photons might be emitted from the surface of the neutron star (NS) or the boundary layer between the NS and the disk and, therefore, this process could take place around the NS or in the boundary layer.

SALT high resolution spectroscopy of GX339-4 in outburst

NASA Astrophysics Data System (ADS)

Buckley, D. A. H.; Aydi, E.; Kotze, M. M.; Gandhi, P.; Altamirano, D.; Charles, P. A.; Russell, D.

2017-10-01

High resolution (R = 15,000) spectroscopy of the current outbursting black hole transient GX339-4 (ATel #10797) was obtained with the SALT High Resolution Spectrograph (HRS; Crause et al. 2014, Proc SPIE, 91476) on 2017-09-29 starting at 17:28 UTC, during evening twilight.

Is there a highly magnetized neutron star in GX 301-2?

NASA Astrophysics Data System (ADS)

Doroshenko, V.; Santangelo, A.; Suleimanov, V.; Kreykenbohm, I.; Staubert, R.; Ferrigno, C.; Klochkov, D.

2010-06-01

We present the results of an in-depth study of the long-period X-ray pulsar GX 301-2. Using archival data of INTEGRAL, RXTE ASM, and CGRO BATSE, we study the spectral and timing properties of the source. Comparison of our timing results with previously published work reveals a secular decay of the orbital period at a rate of ≃ - 3.25 × 10-5 d yr-1, which is an order of magnitude faster than for other known systems. We argue that this is probably result either of the apsidal motion or of gravitational coupling of the matter lost by the optical companion with the neutron star, although current observations do not allow us to distinguish between those possibilities. We also propose a model to explain the observed long pulse period. We find that a very strong magnetic field B ~ 1014 G can explain the observed pulse period in the framework of existing models for torques affecting the neutron star. We show that the apparent contradiction with the magnetic field strength BCRSF ~ 4 × 1012 G derived from the observed cyclotron line position may be resolved if the line formation region resides in a tall accretion column of height ~2.5-3 RNS. The color temperature measured from the spectrum suggests that such a column may indeed be present, and our estimates show that its height is sufficient to explain the observed cyclotron line position.

VizieR Online Data Catalog: IR-bright MSX sources in the SMC with Spitzer/IRS (Kraemer+, 2017)

NASA Astrophysics Data System (ADS)

Kraemer, K. E.; Sloan, G. C.; Wood, P. R.; Jones, O. C.; Egan, M. P.

2017-07-01

Our original set of infrared spectra of MSX SMC sources was obtained in Spitzer Cycle 1 (Program ID 3277, P.I. M. Egan). This program included 35 targets from the MSX SMC catalog. 24 targets were discussed in previous papers; this paper examines the remaining 11 sources in the sample. We also selected 4 objects in the MSX SMC catalog with similar photometric characteristics in an effort to uncover additional sources with crystalline dust. We observed these targets in Spitzer Cycle 3 (Program ID 30355, P.I. J. Houck). See tables 1 and 2 for observation data and basic properties of the targets. Table 3 lists 20 additional MSX SMC sources that were observed by other Spitzer IRS programs. Overall, 59 MSX SMC sources were observed with the IRS. The spectra were observed using the low-resolution modules of the IRS, Short-Low (SL) and Long-Low (LL), which provided spectra in the 5-14 and 14-37um ranges, respectively, at a resolution between ~60 and 120. For 10 evolved stars with oxygen-rich dust in our Cycle 1 program, we obtained spectra from 0.45 to 1.03um with the Double-Beam Spectrograph at the 2.3m telescope of the Australian National University at Siding Spring Observatory. A 0.45-0.89um spectrum for one of the stars in program 30355 was also observed. These spectra have a resolution of 10Å. Tables 5-7: catalog based on the 243 sources detected in the MSX survey of the SMC, updated with positions and photometry from more recent space-based missions and ground-based surveys. See the Appendix section for more details. The SMC catalog from MSX consists of the 243 sources in the main MSX catalog (Egan+ 2003, see V/114) that lie within the region 7°

Heart rate and pulmonary function while wearing the launch-entry crew escape suit (LES) during + Gx acceleration and simulated Shuttle launch

NASA Technical Reports Server (NTRS)

Krutz, Robert W., Jr.; Bagian, James P.; Burton, Russell R.; Meeker, Larry J.

1990-01-01

Space shuttle crewmembers have been equipped with a launch-entry crew escape system (LES) since the Challenger accident in 1986. Some crewmembers, wearing the new pressure suit, have reported breathing difficulties and increased effort to achieve the desired range of motion. This study was conducted to quantify the reported increased physical workloads and breathing difficulty associated with wearing the LES. Both veteran astronauts and centrifuge panel members were exposed to various + Gx profiles (including simulated shuttle launch) + Gx on the USAF School of Aerospace Medicine (USAFSAM) human-use centrifuge. Maximum heart rate data showed no increased workload associated with arm and head movement in the LES when compared to the flight suit/helmet ensemble (LEH). However, the LES did impose a significant increase in breathing difficulty beginning at +2.5 Gx which was demonstrated by a decrease in forced vital capacity and subjected questionnaries.

Blood-Based Detection of Radiation Exposure in Humans Based on Novel Phospho-Smc1 ELISA

PubMed Central

Ivey, Richard G.; Moore, Heather D.; Voytovich, Uliana J.; Thienes, Cortlandt P.; Lorentzen, Travis D.; Pogosova-Agadjanyan, Era L.; Frayo, Shani; Izaguirre, Venissa K.; Lundberg, Sally J.; Hedin, Lacey; Badiozamani, Kas Ray; Hoofnagle, Andrew N.; Stirewalt, Derek L.; Wang, Pei; Georges, George E.; Gopal, Ajay K.; Paulovich, Amanda G.

2011-01-01

The structural maintenance of chromosome 1 (Smc1) protein is a member of the highly conserved cohesin complex and is involved in sister chromatid cohesion. In response to ionizing radiation, Smc1 is phosphorylated at two sites, Ser-957 and Ser-966, and these phosphorylation events are dependent on the ATM protein kinase. In this study, we describe the generation of two novel ELISAs for quantifying phospho-Smc1Ser-957 and phospho-Smc1Ser-966. Using these novel assays, we quantify the kinetic and biodosimetric responses of human cells of hematological origin, including immortalized cells, as well as both quiescent and cycling primary human PBMC. Additionally, we demonstrate a robust in vivo response for phospho-Smc1Ser-957 and phospho-Smc1Ser-966 in lymphocytes of human patients after therapeutic exposure to ionizing radiation, including total-body irradiation, partial-body irradiation, and internal exposure to 131I. These assays are useful for quantifying the DNA damage response in experimental systems and potentially for the identification of individuals exposed to radiation after a radiological incident. PMID:21388270

GX 9+9: VARIABILITY OF THE X-RAY ORBITAL MODULATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Harris, Robert J.; Levine, Alan M.; Durant, Martin

2009-05-10

Results of observations of the Galactic bulge X-ray source GX 9+9 by the All-Sky Monitor (ASM) and Proportional Counter Array (PCA) onboard the Rossi X-ray Timing Explorer are presented. The ASM results show that the 4.19 hr X-ray periodicity first reported by Hertz and Wood in 1987 was weak or not detected for most of the mission prior to late 2004, but then became strong and remained strong for approximately two years after which it weakened considerably. When the modulation at the 4.19 hr period is strong, it appears in folded light curves as an intensity dip over {approx}<30% ofmore » a cycle and is distinctly nonsinusoidal. A number of PCA observations of GX 9+9 were performed before the appearance of strong modulation; two were performed in 2006 during the epoch of strong modulation. Data obtained from the earlier PCA observations yield, at best, limited evidence of the presence of phase-dependent intensity changes, while the data from the later observations confirm the presence of flux minima with depths and phases compatible with those apparent in folded ASM light curves. Light curves from a Chandra observation of GX 9+9 performed in the year 2000 prior to the start of strong modulation show the possible presence of shallow dips at the predicted times. Optical observations performed in 2006 while the X-ray modulation was strong do not show an increase in the degree of modulation at the 4.19 hr period. Implications of the changes in modulation strength in X-rays and other observational results are considered.« less

In vitro resistance to 5-nitroimidazoles and benzimidazoles in Giardia duodenalis: variability and variation in gene expression.

PubMed

Argüello-García, Raúl; Cruz-Soto, Maricela; Romero-Montoya, Lydia; Ortega-Pierres, Guadalupe

2009-12-01

The susceptibility of Giardia duodenalis trophozoites exposed in vitro to sublethal concentrations of metronidazole (MTZ) and albendazole (ABZ) may exhibit inter-culture (variability) and intra-culture (variation) differences in drug susceptibility. It was previously reported that MTZ-resistant trophozoites may display changes in pyruvate:ferredoxin oxidoreductase (PFOR) expression while changes at the beta-tubulin molecule are apparently absent in ABZ-resistant cultures. To assess the levels of gene expression of these molecules, we obtained cloned cultures growing at concentrations up to 23 microM MTZ (WBRM23) and up to 8muM ABZ (WBRA8) and gene sequence and expression of pfor and beta-tubulin loci were compared with these of drug-susceptible clone WB1. Neither the pfor nor the beta-tubulin genes showed changes at sequence level but the MTZ-resistant clones WBRM21 and WBRM23 showed up-regulation of the pfor RNA using the gdh gene as reference. By using WB1 and WBRA8 clones in representational difference analyses of gene expression (RDA) an insert referred to as ARR-VSP was selected and sequenced. It showed the highest homology to one VSP molecule in the Giardia Genome Database (orf GL50803_101765). This isogene was up-regulated in five ABZ-resistant clones and the clone WBRA8 exhibited the highest RNA expression level. When successive progenies of clones WB1, WBRM23 and WBRA8 were analyzed in Northern blot assays to detect pfor and ARR-VSP RNAs respectively, the expression patterns showed variation for both genes but it was much lower in the clone WBRA8. These results suggest that G. duodenalis cultures either susceptible or resistant to MTZ and ABZ may display variability and variation at RNA expression levels albeit these were more marked in the MTZ-resistant parasites. These data might have further implications defining major mechanisms involved in drug resistance of Giardia.

Further RIOTS4 Characterization of Field OB Stars in the SMC

NASA Astrophysics Data System (ADS)

Oey, M. S.; Barnes, Jesse R.; Paggeot, Kevin J.; Dorigo Jones, John; Castro, Norberto; Simon-Diaz, Sergio; Kratter, Kaitlin M.; Moe, Maxwell; Szymanski, Michal

2018-06-01

We present recent results from the Runaways and O-Type Star Spectroscopic Survey of the SMC (RIOTS4), a survey quantifying properties of the field OB stars in the Small Magellanic Cloud (SMC). Based on PSF-fitting photometry and astrometry of OGLE-III I-band images, we quantify the degree of isolation for the target OB stars, classifying them as "tip-of-the-iceberg" stars accompanied by small, sparse, clusters; or as true, isolated field stars. Many of these field stars must be runaways, which we evaluate using GAIA DR2 proper motions. We measure v sin i using the IACOB code Fourier analysis, finding that the bimodal distribution of projected rotation velocities is less pronounced for O stars than early B stars. We examine rotation in relation to relative isolation and runaway status.

Spectra from the IRS of Bright Oxygen-Rich Evolved Stars in the SMC

NASA Astrophysics Data System (ADS)

Kraemer, Kathleen E.; Sloan, Greg; Wood, Peter

2016-06-01

We have used Spitzer's Infrared Spectrograph (IRS) to obtain spectra of stars in the Small Magellanic Cloud (SMC). The targets were chosen from the Point Source Catalog of the Mid-Course Space Experiment (MSX), which detected the 243 brightest infrared sources in the SMC. Our SMC sample of oxygen-rich evolved stars shows more dust than found in previous samples, and the dust tends to be dominated by silicates, with little contribution from alumina. Both results may arise from the selection bias in the MSX sample and our sample toward more massive stars. Additionally, several sources show peculiar spectral features such as PAHs, crystalline silicates, or both carbon-rich and silicate features. The spectrum of one source, MSX SMC 145, is a combination of an ordinary AGB star and a background galaxy at z~0.16, rather than an OH/IR star as previously suggested.

Discovery of the near-infrared counterpart to the luminous neutron-star low-mass X-ray binary GX 3+1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Van den Berg, Maureen; Fridriksson, Joel K.; Homan, Jeroen

2014-10-01

Using the High Resolution Camera on board the Chandra X-ray Observatory, we have measured an accurate position for the bright persistent neutron star X-ray binary and atoll source GX 3+1. At a location that is consistent with this new position, we have discovered the near-infrared (NIR) counterpart to GX 3+1 in images taken with the PANIC and FourStar cameras on the Magellan Baade Telescope. The identification of this K{sub s} = 15.8 ± 0.1 mag star as the counterpart is based on the presence of a Br γ emission line in an NIR spectrum taken with the Folded-port InfraRed Echelettemore » spectrograph on the Baade Telescope. The absolute magnitude derived from the best available distance estimate to GX 3+1 indicates that the mass donor in the system is not a late-type giant. We find that the NIR light in GX 3+1 is likely dominated by the contribution from a heated outer accretion disk. This is similar to what has been found for the NIR flux from the brighter class of Z sources, but unlike the behavior of atolls fainter (L{sub X} ≈ 10{sup 36}-10{sup 37} erg s{sup –1}) than GX 3+1, where optically thin synchrotron emission from a jet probably dominates the NIR flux.« less

The Mass Function of GX 339-4 from Spectroscopic Observations of Its Donor Star

NASA Astrophysics Data System (ADS)

Heida, M.; Jonker, P. G.; Torres, M. A. P.; Chiavassa, A.

2017-09-01

We obtained 16 VLT/X-shooter observations of GX 339-4 in quiescence during the period 2016 May-September and detected absorption lines from the donor star in its NIR spectrum. This allows us to measure the radial velocity curve and projected rotational velocity of the donor for the first time. We confirm the 1.76 day orbital period and we find that K 2 = 219 ± 3 km s-1, γ = 26 ± 2 km s-1, and v\\sin I = 64 ± 8 km s-1. From these values we compute a mass function f(M) = 1.91 ± 0.08 {M}⊙ , a factor ˜3 lower than previously reported, and a mass ratio q = 0.18 ± 0.05. We confirm the donor is a K-type star and estimate that it contributes ˜ 4 % {--}50 % of the light in the J- and H-bands. We constrain the binary inclination to 37° < I < 78° and the black hole (BH) mass to 2.3 {M}⊙ < {M}{BH} < 9.5 {M}⊙ . GX 339-4 may therefore be the first BH to fall in the “mass-gap” of 2-5 M ⊙. Based on ESO program IDs 097.D-0915 and 297.D-5048.

GxG epistasis in growth and condition and the maintenance of genetic polymorphism in Gambusia holbrooki.

PubMed

Culumber, Zachary W; Kraft, Brittany; Lemakos, Valerie; Hoffner, Erika; Travis, Joseph; Hughes, Kimberly A

2018-05-01

Theory on indirect genetic effects (IGEs) indicates that variation in the genetic composition of social groups can generate GxG epistasis that may promote the evolution of stable polymorphisms. Using a livebearing fish with a genetic polymorphism in coloration and associated behavioral differences, we tested whether genotypes of social partners interacted with focal individual genotypes to influence growth and condition over 16 weeks of development. We found that IGEs had a significant influence on patterns of feeding, regardless of focal fish genotype. There was no influence of social environment on juvenile length, but there was significant GxG epistasis for body condition. Each focal juvenile was in better condition when its own genotype was not present in adult social partners. These data are consistent with negative frequency-dependent selection in which each morph performs better when it is rare. Neither variation in feeding nor activity-related behaviors explained variation in body condition, suggesting that GxG epistasis for condition was caused by physiological differences between the two genotypes. These findings indicate that GxG epistasis in a given polymorphism can generate fitness landscapes that contribute to the maintenance of that polymorphism and to maintenance of genetic variation for additional fitness-related traits. © 2018 The Author(s). Evolution © 2018 The Society for the Study of Evolution.

SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes

PubMed Central

Hwang, Grace; Sun, Fengyun; Eppig, John J.; Handel, Mary Ann

2017-01-01

SMC complexes include three major classes: cohesin, condensin and SMC5/6. However, the localization pattern and genetic requirements for the SMC5/6 complex during mammalian oogenesis have not previously been examined. In mouse oocytes, the SMC5/6 complex is enriched at the pericentromeric heterochromatin, and also localizes along chromosome arms during meiosis. The infertility phenotypes of females with a Zp3-Cre-driven conditional knockout (cKO) of Smc5 demonstrated that maternally expressed SMC5 protein is essential for early embryogenesis. Interestingly, protein levels of SMC5/6 complex components in oocytes decline as wild-type females age. When SMC5/6 complexes were completely absent in oocytes during meiotic resumption, homologous chromosomes failed to segregate accurately during meiosis I. Despite what appears to be an inability to resolve concatenation between chromosomes during meiosis, localization of topoisomerase IIα to bivalents was not affected; however, localization of condensin along the chromosome axes was perturbed. Taken together, these data demonstrate that the SMC5/6 complex is essential for the formation of segregation-competent bivalents during meiosis I, and findings suggest that age-dependent depletion of the SMC5/6 complex in oocytes could contribute to increased incidence of oocyte aneuploidy and spontaneous abortion in aging females. PMID:28302748

A fault-tolerant strategy based on SMC for current-controlled converters

NASA Astrophysics Data System (ADS)

Azer, Peter M.; Marei, Mostafa I.; Sattar, Ahmed A.

2018-05-01

The sliding mode control (SMC) is used to control variable structure systems such as power electronics converters. This paper presents a fault-tolerant strategy based on the SMC for current-controlled AC-DC converters. The proposed SMC is based on three sliding surfaces for the three legs of the AC-DC converter. Two sliding surfaces are assigned to control the phase currents since the input three-phase currents are balanced. Hence, the third sliding surface is considered as an extra degree of freedom which is utilised to control the neutral voltage. This action is utilised to enhance the performance of the converter during open-switch faults. The proposed fault-tolerant strategy is based on allocating the sliding surface of the faulty leg to control the neutral voltage. Consequently, the current waveform is improved. The behaviour of the current-controlled converter during different types of open-switch faults is analysed. Double switch faults include three cases: two upper switch fault; upper and lower switch fault at different legs; and two switches of the same leg. The dynamic performance of the proposed system is evaluated during healthy and open-switch fault operations. Simulation results exhibit the various merits of the proposed SMC-based fault-tolerant strategy.

Sliding Mode Control (SMC) of Robot Manipulator via Intelligent Controllers

NASA Astrophysics Data System (ADS)

Kapoor, Neha; Ohri, Jyoti

2017-02-01

Inspite of so much research, key technical problem, naming chattering of conventional, simple and robust SMC is still a challenge to the researchers and hence limits its practical application. However, newly developed soft computing based techniques can provide solution. In order to have advantages of conventional and heuristic soft computing based control techniques, in this paper various commonly used intelligent techniques, neural network, fuzzy logic and adaptive neuro fuzzy inference system (ANFIS) have been combined with sliding mode controller (SMC). For validation, proposed hybrid control schemes have been implemented for tracking a predefined trajectory by robotic manipulator, incorporating structured and unstructured uncertainties in the system. After reviewing numerous papers, all the commonly occurring uncertainties like continuous disturbance, uniform random white noise, static friction like coulomb friction and viscous friction, dynamic friction like Dhal friction and LuGre friction have been inserted in the system. Various performance indices like norm of tracking error, chattering in control input, norm of input torque, disturbance rejection, chattering rejection have been used. Comparative results show that with almost eliminated chattering the intelligent SMC controllers are found to be more efficient over simple SMC. It has also been observed from results that ANFIS based controller has the best tracking performance with the reduced burden on the system. No paper in the literature has found to have all these structured and unstructured uncertainties together for motion control of robotic manipulator.

A new twist in the coil: functions of the coiled-coil domain of structural maintenance of chromosome (SMC) proteins.

PubMed

Matityahu, Avi; Onn, Itay

2018-02-01

The higher-order organization of chromosomes ensures their stability and functionality. However, the molecular mechanism by which higher order structure is established is poorly understood. Dissecting the activity of the relevant proteins provides information essential for achieving a comprehensive understanding of chromosome structure. Proteins of the structural maintenance of chromosome (SMC) family of ATPases are the core of evolutionary conserved complexes. SMC complexes are involved in regulating genome dynamics and in maintaining genome stability. The structure of all SMC proteins resembles an elongated rod that contains a central coiled-coil domain, a common protein structural motif in which two α-helices twist together. In recent years, the imperative role of the coiled-coil domain to SMC protein activity and regulation has become evident. Here, we discuss recent advances in the function of the SMC coiled coils. We describe the structure of the coiled-coil domain of SMC proteins, modifications and interactions that are mediated by it. Furthermore, we assess the role of the coiled-coil domain in conformational switches of SMC proteins, and in determining the architecture of the SMC dimer. Finally, we review the interplay between mutations in the coiled-coil domain and human disorders. We suggest that distinctive properties of coiled coils of different SMC proteins contribute to their distinct functions. The discussion clarifies the mechanisms underlying the activity of SMC proteins, and advocates future studies to elucidate the function of the SMC coiled coil domain.

A NICER View of the Accretion Disk in GX 339-4

NASA Astrophysics Data System (ADS)

Steiner, James Francis; Bulbul, Esra; Cackett, Ed; Fabian, Andy; Gendreau, Keith C.; Neilsen, Joseph; Ranga Reddy Pasham, Dheeraj; Remillard, Ron; Uttley, Phil; Wood, Kent S.

2018-01-01

The poster-child black hole transient GX 339-4 has gone into outburst once again. With no pileup, low-background, and high fidelity in the soft X-ray bandpass, NICER is uniquely positioned to detect emergent thermal disk emission from an optically thick accretion flow approaching the innermost-stable circular orbit. We present NICER's results on the 2017 outburst, and detail its implications for the disk-truncation controversy. We also investigate the X-ray state evolution, as seen in NICER's spectral range of 0.2 to 12 keV.

9 CFR 2.54 - Lost tags.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

9 CFR 2.54 - Lost tags.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

9 CFR 2.54 - Lost tags.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

9 CFR 2.54 - Lost tags.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

9 CFR 2.54 - Lost tags.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Lost tags. 2.54 Section 2.54 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE ANIMAL WELFARE REGULATIONS Identification of Animals § 2.54 Lost tags. Each dealer or exhibitor shall be held...

Gx1-conjugated endostar nanoparticle: a new drug delivery system for anti-colorectal cancer in vivo

NASA Astrophysics Data System (ADS)

Zhang, Qian; Du, Yang; Li, Yaqian; Liang, Xiaolong; Yang, Xin; Tian, Jie

2014-03-01

In this study we describe a new theranostic nanostytem to combine those functions together. GX1, the peptide identified by phage display technology, is a tumor vasculature endothelium specific ligand. Endostar, a novel recombinant human endostatin, has been proved to inhibit tumor angiogenesis. In this study, Endostar-loaded PLA nanoparticles (EPNPs) were first prepared, and then GX1 was coupled to the surface of EPNPs for targeting therapy, last a near infrared (NIR) dye IRDye 800CW was conjugated to the surface of EPNPs for monitoring the biodistributon. This GX1-EPNPs-NIR dye IRDye 800CW (GEN) multifunction drug delivery system not only facilitates efficient delivery of chemotherapeutic agents to tumor site, while minimizing systemic toxicity and side effects, but also enables to real time monitor tumor targeting in vivo. Compare to the Endostar and EPNPs, the GEN inhibited the subcutaneous colon tumor more obviously both in tumor volume and bioluminescence imaging (BLI) light intensity during the 10 days drug treatment.

The high-energy X-ray spectrum of black hole candidate GX 339-4 during a transition

NASA Technical Reports Server (NTRS)

Dolan, J. F.; Crannell, C. J.; Dennis, B. R.; Orwig, L. E.

1987-01-01

The X-ray emitting system GX 339-4 contains one of the prime candidates for a stellar mass-sized black hole. Determining the observational similarities and differences between the members of this group is of value in specifying which characteristics can be used to identify systems containing a black hole, especially those for which no mass determination can be made. The first observations of the E greater than 20 keV spectrum of GX 339-4 during a transition between luminosity states are reported here. The hard spectral state is the lower luminosity state of the system. GX 339-4 has a power-low spectrum above 20 keV which pivots during transitions between distinct luminosity states. The only other X-ray sources known to exhibit this behavior, Cyg XR-1 and (probably) A0620-00, are leading candidates for systems containing a black hole component based on their measured spectrocopic mass function.

A jet model for a very high state of GX 339 - 4

DOE Office of Scientific and Technical Information (OSTI.GOV)

Miyamoto, Sigenori; Kitamoto, Shunji

1991-06-01

A jet model is proposed which explain results derived by Ginga observation of GX 339 {minus} 4 in its very high state. Specifically, the model can explain: (1) the possible existence of large Compton clouds of 1-billion-cm size; (2) the independent change of the soft and hard components in the long term; (3) the rapid variability of the hard tail component in the short term; and (4) the possible existence of absorbing matter around the compact star. 25 refs.

SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes.

PubMed

Hwang, Grace; Sun, Fengyun; O'Brien, Marilyn; Eppig, John J; Handel, Mary Ann; Jordan, Philip W

2017-05-01

SMC complexes include three major classes: cohesin, condensin and SMC5/6. However, the localization pattern and genetic requirements for the SMC5/6 complex during mammalian oogenesis have not previously been examined. In mouse oocytes, the SMC5/6 complex is enriched at the pericentromeric heterochromatin, and also localizes along chromosome arms during meiosis. The infertility phenotypes of females with a Zp3-Cre -driven conditional knockout (cKO) of Smc5 demonstrated that maternally expressed SMC5 protein is essential for early embryogenesis. Interestingly, protein levels of SMC5/6 complex components in oocytes decline as wild-type females age. When SMC5/6 complexes were completely absent in oocytes during meiotic resumption, homologous chromosomes failed to segregate accurately during meiosis I. Despite what appears to be an inability to resolve concatenation between chromosomes during meiosis, localization of topoisomerase IIα to bivalents was not affected; however, localization of condensin along the chromosome axes was perturbed. Taken together, these data demonstrate that the SMC5/6 complex is essential for the formation of segregation-competent bivalents during meiosis I, and findings suggest that age-dependent depletion of the SMC5/6 complex in oocytes could contribute to increased incidence of oocyte aneuploidy and spontaneous abortion in aging females. © 2017. Published by The Company of Biologists Ltd.

Coordinating IMC-PID and adaptive SMC controllers for a PEMFC.

PubMed

Wang, Guo-Liang; Wang, Yong; Shi, Jun-Hai; Shao, Hui-He

2010-01-01

For a Proton Exchange Membrane Fuel Cell (PEMFC) power plant with a methanol reformer, the process parameters and power output are considered simultaneously to avoid violation of the constraints and to keep the fuel cell power plant safe and effective. In this paper, a novel coordinating scheme is proposed by combining an Internal Model Control (IMC) based PID Control and adaptive Sliding Mode Control (SMC). The IMC-PID controller is designed for the reformer of the fuel flow rate according to the expected first-order dynamic properties. The adaptive SMC controller of the fuel cell current has been designed using the constant plus proportional rate reaching law. The parameters of the SMC controller are adaptively tuned according to the response of the fuel flow rate control system. When the power output controller feeds back the current references to these two controllers, the coordinating controllers system works in a system-wide way. The simulation results of the PEMFC power plant demonstrate the effectiveness of the proposed method. 2009 ISA. Published by Elsevier Ltd. All rights reserved.

Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease

PubMed Central

van der Crabben, Saskia N.; Hennus, Marije P.; McGregor, Grant A.; Ritter, Deborah I.; Nagamani, Sandesh C.S.; Wells, Owen S.; Harakalova, Magdalena; Chinn, Ivan K.; Alt, Aaron; Vondrova, Lucie; Hochstenbach, Ron; van Montfrans, Joris M.; Terheggen-Lagro, Suzanne W.; van Lieshout, Stef; van Roosmalen, Markus J.; Renkens, Ivo; Duran, Karen; Nijman, Isaac J.; Kloosterman, Wigard P.; Hennekam, Eric; van Hasselt, Peter M.; Wheeler, David A.; Palecek, Jan J.; Lehmann, Alan R.; Oliver, Antony W.; Pearl, Laurence H.; Plon, Sharon E.; Murray, Johanne M.

2016-01-01

The structural maintenance of chromosomes (SMC) family of proteins supports mitotic proliferation, meiosis, and DNA repair to control genomic stability. Impairments in chromosome maintenance are linked to rare chromosome breakage disorders. Here, we have identified a chromosome breakage syndrome associated with severe lung disease in early childhood. Four children from two unrelated kindreds died of severe pulmonary disease during infancy following viral pneumonia with evidence of combined T and B cell immunodeficiency. Whole exome sequencing revealed biallelic missense mutations in the NSMCE3 (also known as NDNL2) gene, which encodes a subunit of the SMC5/6 complex that is essential for DNA damage response and chromosome segregation. The NSMCE3 mutations disrupted interactions within the SMC5/6 complex, leading to destabilization of the complex. Patient cells showed chromosome rearrangements, micronuclei, sensitivity to replication stress and DNA damage, and defective homologous recombination. This work associates missense mutations in NSMCE3 with an autosomal recessive chromosome breakage syndrome that leads to defective T and B cell function and acute respiratory distress syndrome in early childhood. PMID:27427983

Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease.

PubMed

van der Crabben, Saskia N; Hennus, Marije P; McGregor, Grant A; Ritter, Deborah I; Nagamani, Sandesh C S; Wells, Owen S; Harakalova, Magdalena; Chinn, Ivan K; Alt, Aaron; Vondrova, Lucie; Hochstenbach, Ron; van Montfrans, Joris M; Terheggen-Lagro, Suzanne W; van Lieshout, Stef; van Roosmalen, Markus J; Renkens, Ivo; Duran, Karen; Nijman, Isaac J; Kloosterman, Wigard P; Hennekam, Eric; Orange, Jordan S; van Hasselt, Peter M; Wheeler, David A; Palecek, Jan J; Lehmann, Alan R; Oliver, Antony W; Pearl, Laurence H; Plon, Sharon E; Murray, Johanne M; van Haaften, Gijs

2016-08-01

The structural maintenance of chromosomes (SMC) family of proteins supports mitotic proliferation, meiosis, and DNA repair to control genomic stability. Impairments in chromosome maintenance are linked to rare chromosome breakage disorders. Here, we have identified a chromosome breakage syndrome associated with severe lung disease in early childhood. Four children from two unrelated kindreds died of severe pulmonary disease during infancy following viral pneumonia with evidence of combined T and B cell immunodeficiency. Whole exome sequencing revealed biallelic missense mutations in the NSMCE3 (also known as NDNL2) gene, which encodes a subunit of the SMC5/6 complex that is essential for DNA damage response and chromosome segregation. The NSMCE3 mutations disrupted interactions within the SMC5/6 complex, leading to destabilization of the complex. Patient cells showed chromosome rearrangements, micronuclei, sensitivity to replication stress and DNA damage, and defective homologous recombination. This work associates missense mutations in NSMCE3 with an autosomal recessive chromosome breakage syndrome that leads to defective T and B cell function and acute respiratory distress syndrome in early childhood.

Control of Smc Coiled Coil Architecture by the ATPase Heads Facilitates Targeting to Chromosomal ParB/parS and Release onto Flanking DNA

PubMed Central

Minnen, Anita; Bürmann, Frank; Wilhelm, Larissa; Anchimiuk, Anna; Diebold-Durand, Marie-Laure; Gruber, Stephan

2016-01-01

Summary Smc/ScpAB promotes chromosome segregation in prokaryotes, presumably by compacting and resolving nascent sister chromosomes. The underlying mechanisms, however, are poorly understood. Here, we investigate the role of the Smc ATPase activity in the recruitment of Smc/ScpAB to the Bacillus subtilis chromosome. We demonstrate that targeting of Smc/ScpAB to ParB/parS loading sites is strictly dependent on engagement of Smc head domains and relies on an open organization of the Smc coiled coils. We find that dimerization of the Smc hinge domain stabilizes closed Smc rods and hinders head engagement as well as chromosomal targeting. Conversely, the ScpAB sub-complex promotes head engagement and Smc rod opening and thereby facilitates recruitment of Smc to parS sites. Upon ATP hydrolysis, Smc/ScpAB is released from loading sites and relocates within the chromosome—presumably through translocation along DNA double helices. Our findings define an intermediate state in the process of chromosome organization by Smc. PMID:26904953

NICER Observation of Fast X-ray Flares in GX 339-4

NASA Astrophysics Data System (ADS)

Remillard, R.; Gendreau, K.; Altamirano, D.; Gandhi, P.; Steiner, J.; Pasham, D.; Charkrabarty, D.; Fabian, A.; Neilsen, J.; Miller, J.; Cackett, E.; Homan, J.; Uttley, P.; Corcoran, M.; Arzoumanian, Z.

2017-10-01

In response to the optical alert reporting the beginning of a new outburst in the black hole binary GX339-4 (D. Russell et al., Atel 10797), a series of NICER observations began on 2017 September 29. The first five observations, conducted through October 1, netted 8.3 ks of exposure.

21 CFR 54.2 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 21 Food and Drugs 1 2013-04-01 2013-04-01 false Definitions. 54.2 Section 54.2 Food and Drugs FOOD... outcome of clinical studies means compensation that could be higher for a favorable outcome than for an unfavorable outcome, such as compensation that is explicitly greater for a favorable result or compensation to...

21 CFR 54.2 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 21 Food and Drugs 1 2012-04-01 2012-04-01 false Definitions. 54.2 Section 54.2 Food and Drugs FOOD... outcome of clinical studies means compensation that could be higher for a favorable outcome than for an unfavorable outcome, such as compensation that is explicitly greater for a favorable result or compensation to...

21 CFR 54.2 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Definitions. 54.2 Section 54.2 Food and Drugs FOOD... outcome of clinical studies means compensation that could be higher for a favorable outcome than for an unfavorable outcome, such as compensation that is explicitly greater for a favorable result or compensation to...

21 CFR 54.2 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 21 Food and Drugs 1 2014-04-01 2014-04-01 false Definitions. 54.2 Section 54.2 Food and Drugs FOOD... outcome of clinical studies means compensation that could be higher for a favorable outcome than for an unfavorable outcome, such as compensation that is explicitly greater for a favorable result or compensation to...

21 CFR 54.2 - Definitions.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 1 2011-04-01 2011-04-01 false Definitions. 54.2 Section 54.2 Food and Drugs FOOD... outcome of clinical studies means compensation that could be higher for a favorable outcome than for an unfavorable outcome, such as compensation that is explicitly greater for a favorable result or compensation to...

Examining the infrared variable star population discovered in the Small Magellanic Cloud using the SAGE-SMC survey

DOE Office of Scientific and Technical Information (OSTI.GOV)

Polsdofer, Elizabeth; Marengo, M.; Seale, J.

2015-02-01

We present our study on the infrared variability of point sources in the Small Magellanic Cloud (SMC). We use the data from the Spitzer Space Telescope Legacy Program “Surveying the Agents of Galaxy Evolution in the Tidally Stripped, Low Metallicity Small Magellanic Cloud” (SAGE-SMC) and the “Spitzer Survey of the Small Magellanic Cloud” (S{sup 3}MC) survey, over three different epochs, separated by several months to 3 years. Variability in the thermal infrared is identified using a combination of Spitzer’s InfraRed Array Camera 3.6, 4.5, 5.8, and 8.0 μm bands, and the Multiband Imaging Photometer for Spitzer 24 μm band. Anmore » error-weighted flux difference between each pair of three epochs (“variability index”) is used to assess the variability of each source. A visual source inspection is used to validate the photometry and image quality. Out of ∼2 million sources in the SAGE-SMC catalog, 814 meet our variability criteria. We matched the list of variable star candidates to the catalogs of SMC sources classified with other methods, available in the literature. Carbon-rich Asymptotic Giant Branch (AGB) stars make up the majority (61%) of our variable sources, with about a third of all of our sources being classified as extreme AGB stars. We find a small, but significant population of oxygen-rich (O-rich) AGB (8.6%), Red Supergiant (2.8%), and Red Giant Branch (<1%) stars. Other matches to the literature include Cepheid variable stars (8.6%), early type stars (2.8%), Young-stellar objects (5.8%), and background galaxies (1.2%). We found a candidate OH maser star, SSTISAGE1C J005212.88-730852.8, which is a variable O-rich AGB star, and would be the first OH/IR star in the SMC, if confirmed. We measured the infrared variability of a rare RV Tau variable (a post-AGB star) that has recently left the AGB phase. 59 variable stars from our list remain unclassified.« less

SMC1-Mediated Intra-S-Phase Arrest Facilitates Bocavirus DNA Replication

PubMed Central

Luo, Yong; Deng, Xuefeng; Cheng, Fang; Li, Yi

2013-01-01

Activation of a host DNA damage response (DDR) is essential for DNA replication of minute virus of canines (MVC), a member of the genus Bocavirus of the Parvoviridae family; however, the mechanism by which DDR contributes to viral DNA replication is unknown. In the current study, we demonstrate that MVC infection triggers the intra-S-phase arrest to slow down host cellular DNA replication and to recruit cellular DNA replication factors for viral DNA replication. The intra-S-phase arrest is regulated by ATM (ataxia telangiectasia-mutated kinase) signaling in a p53-independent manner. Moreover, we demonstrate that SMC1 (structural maintenance of chromosomes 1) is the key regulator of the intra-S-phase arrest induced during infection. Either knockdown of SMC1 or complementation with a dominant negative SMC1 mutant blocks both the intra-S-phase arrest and viral DNA replication. Finally, we show that the intra-S-phase arrest induced during MVC infection was caused neither by damaged host cellular DNA nor by viral proteins but by replicating viral genomes physically associated with the DNA damage sensor, the Mre11-Rad50-Nbs1 (MRN) complex. In conclusion, the feedback loop between MVC DNA replication and the intra-S-phase arrest is mediated by ATM-SMC1 signaling and plays a critical role in MVC DNA replication. Thus, our findings unravel the mechanism underlying DDR signaling-facilitated MVC DNA replication and demonstrate a novel strategy of DNA virus-host interaction. PMID:23365434

Modeling and Simulation of Compression Molding Process for Sheet Molding Compound (SMC) of Chopped Carbon Fiber Composites

DOE PAGES

Li, Yang; Chen, Zhangxing; Xu, Hongyi; ...

2017-01-02

Compression molded SMC composed of chopped carbon fiber and resin polymer which balances the mechanical performance and manufacturing cost presents a promising solution for vehicle lightweight strategy. However, the performance of the SMC molded parts highly depends on the compression molding process and local microstructure, which greatly increases the cost for the part level performance testing and elongates the design cycle. ICME (Integrated Computational Material Engineering) approaches are thus necessary tools to reduce the number of experiments required during part design and speed up the deployment of the SMC materials. As the fundamental stage of the ICME workflow, commercial softwaremore » packages for SMC compression molding exist yet remain not fully validated especially for chopped fiber systems. In this study, SMC plaques are prepared through compression molding process. The corresponding simulation models are built in Autodesk Moldflow with the same part geometry and processing conditions as in the molding tests. The output variables of the compression molding simulations, including press force history and fiber orientation of the part, are compared with experimental data. Influence of the processing conditions to the fiber orientation of the SMC plaque is also discussed. It is found that generally Autodesk Moldflow can achieve a good simulation of the compression molding process for chopped carbon fiber SMC, yet quantitative discrepancies still remain between predicted variables and experimental results.« less

Modeling and Simulation of Compression Molding Process for Sheet Molding Compound (SMC) of Chopped Carbon Fiber Composites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Yang; Chen, Zhangxing; Xu, Hongyi

Compression molded SMC composed of chopped carbon fiber and resin polymer which balances the mechanical performance and manufacturing cost presents a promising solution for vehicle lightweight strategy. However, the performance of the SMC molded parts highly depends on the compression molding process and local microstructure, which greatly increases the cost for the part level performance testing and elongates the design cycle. ICME (Integrated Computational Material Engineering) approaches are thus necessary tools to reduce the number of experiments required during part design and speed up the deployment of the SMC materials. As the fundamental stage of the ICME workflow, commercial softwaremore » packages for SMC compression molding exist yet remain not fully validated especially for chopped fiber systems. In this study, SMC plaques are prepared through compression molding process. The corresponding simulation models are built in Autodesk Moldflow with the same part geometry and processing conditions as in the molding tests. The output variables of the compression molding simulations, including press force history and fiber orientation of the part, are compared with experimental data. Influence of the processing conditions to the fiber orientation of the SMC plaque is also discussed. It is found that generally Autodesk Moldflow can achieve a good simulation of the compression molding process for chopped carbon fiber SMC, yet quantitative discrepancies still remain between predicted variables and experimental results.« less

Age-dependent seizures of absence epilepsy and sleep spindles dynamics in WAG/Rij rats

NASA Astrophysics Data System (ADS)

Grubov, Vadim V.; Sitnikova, Evgenia Y.; Pavlov, Alexey N.; Khramova, Marina V.; Koronovskii, Alexey A.; Hramov, Alexander E.

2015-03-01

In the given paper, a relation between time-frequency characteristics of sleep spindles and the age-dependent epileptic activity in WAG/Rij rats is discussed. Analysis of sleep spindles based on the continuous wavelet transform is performed for rats of different ages. It is shown that the epileptic activity affects the time-frequency intrinsic dynamics of sleep spindles.

40 CFR Appendix - Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC...

Code of Federal Regulations, 2010 CFR

2010-07-01

... Limits for Open Molding, Centrifugal Casting, and SMC Manufacturing Operations Where the Standards Are... CATEGORIES National Emissions Standards for Hazardous Air Pollutants: Reinforced Plastic Composites..., Table 5 Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC...

Score-moment combined linear discrimination analysis (SMC-LDA) as an improved discrimination method.

PubMed

Han, Jintae; Chung, Hoeil; Han, Sung-Hwan; Yoon, Moon-Young

2007-01-01

A new discrimination method called the score-moment combined linear discrimination analysis (SMC-LDA) has been developed and its performance has been evaluated using three practical spectroscopic datasets. The key concept of SMC-LDA was to use not only the score from principal component analysis (PCA), but also the moment of the spectrum, as inputs for LDA to improve discrimination. Along with conventional score, moment is used in spectroscopic fields as an effective alternative for spectral feature representation. Three different approaches were considered. Initially, the score generated from PCA was projected onto a two-dimensional feature space by maximizing Fisher's criterion function (conventional PCA-LDA). Next, the same procedure was performed using only moment. Finally, both score and moment were utilized simultaneously for LDA. To evaluate discrimination performances, three different spectroscopic datasets were employed: (1) infrared (IR) spectra of normal and malignant stomach tissue, (2) near-infrared (NIR) spectra of diesel and light gas oil (LGO) and (3) Raman spectra of Chinese and Korean ginseng. For each case, the best discrimination results were achieved when both score and moment were used for LDA (SMC-LDA). Since the spectral representation character of moment was different from that of score, inclusion of both score and moment for LDA provided more diversified and descriptive information.

X-ray brightening of GX 339-4 in late September 2017

NASA Astrophysics Data System (ADS)

Gandhi, P.; Altamirano, D.; Russell, D. M.; Knigge, C.; Middleton, M.; Veledina, A.; Beri, A.; Paice, J.

2017-09-01

Following the report of optical brightening of the Galactic black hole X-ray transient GX 339-4 (ATel #10797), we requested Swift follow-up of the source. The X-Ray Telescope (XRT; Burrows et al. 2005 SSRv 120 165) data products from the automated pipeline (Evans et al. 2009 MNRAS 397 1177) show the source to be clearly detected.

Antisymmetric Amino-Wagging Band of Hydrazine up toK‧ = 13 Levels

NASA Astrophysics Data System (ADS)

Gulaczyk, Iwona; Kre, Marek; Valentin, Alain

1997-12-01

A newly recorded high-resolution infrared spectrum of hydrazine has been studied in the 729-1198 cm-1region (the ν12antisymmetric wagging band) with a resolution of 0.002 cm-1. About 1350 transitions withK‧ from 7 to 13 have been newly assigned and about 2350 transitions with lower values ofK‧ reanalyzed with the improved precision. The effective parameters have been calculated separately for each value ofK‧ using the Hougen-Ohashi hamiltonian for hydrazine. The extended assignment completes the analysis of the ν12band of hydrazine.

High performance SMC matrix for structural applications

NASA Astrophysics Data System (ADS)

Salard, T.; Lortie, F.; Gérard, J. F.; Peyre, C.

2016-07-01

Mechanical properties of a common SMC (Sheet Molding Compound) matrix constituted of a vinylester resin and a Low-Profile Additive (LPA) were compared to those of vinylester modified with core-shell rubber (CSR) particles. Valuable properties are brought by CSR, especially high impact strength, high fracture toughness with little loss in stiffness, in spite of the presence of CSR agglomerates in blends.

Bovine exome sequence analysis and targeted SNP genotyping of recessive fertility defects BH1, HH2, and HH3 reveal a putative causative mutation in SMC2 for HH3.

PubMed

McClure, Matthew C; Bickhart, Derek; Null, Dan; Vanraden, Paul; Xu, Lingyang; Wiggans, George; Liu, George; Schroeder, Steve; Glasscock, Jarret; Armstrong, Jon; Cole, John B; Van Tassell, Curtis P; Sonstegard, Tad S

2014-01-01

The recent discovery of bovine haplotypes with negative effects on fertility in the Brown Swiss, Holstein, and Jersey breeds has allowed producers to identify carrier animals using commercial single nucleotide polymorphism (SNP) genotyping assays. This study was devised to identify the causative mutations underlying defective bovine embryo development contained within three of these haplotypes (Brown Swiss haplotype 1 and Holstein haplotypes 2 and 3) by combining exome capture with next generation sequencing. Of the 68,476,640 sequence variations (SV) identified, only 1,311 genome-wide SNP were concordant with the haplotype status of 21 sequenced carriers. Validation genotyping of 36 candidate SNP identified only 1 variant that was concordant to Holstein haplotype 3 (HH3), while no variants located within the refined intervals for HH2 or BH1 were concordant. The variant strictly associated with HH3 is a non-synonymous SNP (T/C) within exon 24 of the Structural Maintenance of Chromosomes 2 (SMC2) on Chromosome 8 at position 95,410,507 (UMD3.1). This polymorphism changes amino acid 1135 from phenylalanine to serine and causes a non-neutral, non-tolerated, and evolutionarily unlikely substitution within the NTPase domain of the encoded protein. Because only exome capture sequencing was used, we could not rule out the possibility that the true causative mutation for HH3 might lie in a non-exonic genomic location. Given the essential role of SMC2 in DNA repair, chromosome condensation and segregation during cell division, our findings strongly support the non-synonymous SNP (T/C) in SMC2 as the likely causative mutation. The absence of concordant variations for HH2 or BH1 suggests either the underlying causative mutations lie within a non-exomic region or in exome regions not covered by the capture array.

Bovine Exome Sequence Analysis and Targeted SNP Genotyping of Recessive Fertility Defects BH1, HH2, and HH3 Reveal a Putative Causative Mutation in SMC2 for HH3

PubMed Central

McClure, Matthew C.; Bickhart, Derek; Null, Dan; VanRaden, Paul; Xu, Lingyang; Wiggans, George; Liu, George; Schroeder, Steve; Glasscock, Jarret; Armstrong, Jon; Cole, John B.; Van Tassell, Curtis P.; Sonstegard, Tad S.

2014-01-01

The recent discovery of bovine haplotypes with negative effects on fertility in the Brown Swiss, Holstein, and Jersey breeds has allowed producers to identify carrier animals using commercial single nucleotide polymorphism (SNP) genotyping assays. This study was devised to identify the causative mutations underlying defective bovine embryo development contained within three of these haplotypes (Brown Swiss haplotype 1 and Holstein haplotypes 2 and 3) by combining exome capture with next generation sequencing. Of the 68,476,640 sequence variations (SV) identified, only 1,311 genome-wide SNP were concordant with the haplotype status of 21 sequenced carriers. Validation genotyping of 36 candidate SNP identified only 1 variant that was concordant to Holstein haplotype 3 (HH3), while no variants located within the refined intervals for HH2 or BH1 were concordant. The variant strictly associated with HH3 is a non-synonymous SNP (T/C) within exon 24 of the Structural Maintenance of Chromosomes 2 (SMC2) on Chromosome 8 at position 95,410,507 (UMD3.1). This polymorphism changes amino acid 1135 from phenylalanine to serine and causes a non-neutral, non-tolerated, and evolutionarily unlikely substitution within the NTPase domain of the encoded protein. Because only exome capture sequencing was used, we could not rule out the possibility that the true causative mutation for HH3 might lie in a non-exonic genomic location. Given the essential role of SMC2 in DNA repair, chromosome condensation and segregation during cell division, our findings strongly support the non-synonymous SNP (T/C) in SMC2 as the likely causative mutation. The absence of concordant variations for HH2 or BH1 suggests either the underlying causative mutations lie within a non-exomic region or in exome regions not covered by the capture array. PMID:24667746

Use of Pleurotus eous Strain P-31 Spent Mushroom Compost (SMC) as Soil Conditioner on the Growth and Yield Performance of Capsicum annuum L. and Solanum lycopersicon L. Seedlings under Greenhouse Conditions in Ghana

PubMed Central

Wiafe-Kwagyan, M; Odamtten, G T

2018-01-01

The objective of this study was to investigate the influence of spent mushroom compost of Pleurotus eous strain P-31 on the growth and yield performance of pepper and tomato seedlings under greenhouse conditions. Sandy loam soil was combined with different percentages of SMC to obtain the following combinations (0, 5, 10, 15, 20, 25 and 30) %. Lower concentrations SMC5, SMC10 and SMC15 promoted vegetative growth (plant height, leaf area, chlorophyll content, number of leaves and axillary branches) of the two test plants. Tomato seedlings grown in SMC10 recorded the highest plant height (50.3 ± 7.2cm); leaf area (378.8 ± 1.2cm2); number of floral buds (51) and flowers (28) whereas SMC5 recorded the highest chlorophyll content 34.1 ± 0.9CCI though SMC15 recorded the highest number of leaves (8). Tomato seedlings grown in SMC30 produced both the maximum number of fruits (8) with corresponding high weight (34.2 ± 7.7g). Pepper seedlings grown in lower concentrations (SMC5–15) recorded the highest plant heights (29.8–30.8cm), chlorophyll content (20.3CCI) and leaf area (53.5–66.2 cm2). Although the different combinations of sandy loam soil and SMC did not significantly (p ≥ 0.05) affect the number of axillary branches developed; different combinations significantly (p ≤ 0.05) affected the number of floral bud, flower and fruit, weight of fruits formed and value of each of these increased with increasing percentage of SMC. Pepper seedlings grown on SMC30 recorded the maximum number of floral buds (32.0 ± 3.6), number of flowers (19.4 ± 1.3), number of fruits (10.8 ± 1.2) and weight of fruits (31.9 ± 3.4g). Tomato seedlings raised on SMC100 (spent mushroom compost only) and soil only did not significantly (p ≥ 0.05) differ from each other however, was statistically significant (p ≤ 0.05) from amended sandy loam soil by all criteria investigated. The study shows that SMC provide favourable soil conditioners for the cultivation of fruits, vegetables

A Hard Look at the Neutron Stars and Accretion Disks in 4U 1636-53, GX 17+2, and 4U 1705-44 with NuStar

NASA Astrophysics Data System (ADS)

Ludlam, R. M.; Miller, J. M.; Bachetti, M.; Barret, D.; Bostrom, A. C.; Cackett, E. M.; Degenaar, N.; Di Salvo, T.; Natalucci, L.; Tomsick, J. A.; Paerels, F.; Parker, M. L.

2017-02-01

We present NuSTAR observations of neutron star (NS) low-mass X-ray binaries: 4U 1636-53, GX 17+2, and 4U 1705-44. We observed 4U 1636-53 in the hard state, with an Eddington fraction, {F}{Edd}, of 0.01; GX 17+2 and 4U 1705-44 were in the soft state with fractions of 0.57 and 0.10, respectively. Each spectrum shows evidence for a relativistically broadened Fe K α line. Through accretion disk reflection modeling, we constrain the radius of the inner disk in 4U 1636-53 to be {R}{in}=1.03+/- 0.03 ISCO (innermost stable circular orbit), assuming a dimensionless spin parameter {a}* ={cJ}/{{GM}}2=0.0, and {R}{in}=1.08+/- 0.06 ISCO for {a}* =0.3 (errors quoted at 1σ). This value proves to be model independent. For {a}* =0.3 and M=1.4 {M}⊙ , for example, 1.08 ± 0.06 ISCO translates to a physical radius of R=10.8+/- 0.6 km, and the NS would have to be smaller than this radius (other outcomes are possible for allowed spin parameters and masses). For GX 17+2, {R}{in}=1.00{--}1.04 ISCO for {a}* =0.0 and {R}{in}=1.03{--}1.30 ISCO for {a}* =0.3. For {a}* =0.3 and M=1.4 {M}⊙ , {R}{in}=1.03{--}1.30 ISCO translates to R=10.3{--}13.0 km. The inner accretion disk in 4U 1705-44 may be truncated just above the stellar surface, perhaps by a boundary layer or magnetosphere; reflection models give a radius of 1.46-1.64 ISCO for {a}* =0.0 and 1.69-1.93 ISCO for {a}* =0.3. We discuss the implications our results may have on the equation of state of ultradense, cold matter and our understanding of the innermost accretion flow onto NSs with low surface magnetic fields, and systematic errors related to the reflection models and spacetime metric around less idealized NSs.

42 CFR 54a.2 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... financial assistance under an applicable program. (e) SAMHSA means the Substance Abuse and Mental Health... 42 Public Health 1 2010-10-01 2010-10-01 false Definitions. 54a.2 Section 54a.2 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS CHARITABLE CHOICE REGULATIONS...

A Comparative study of two RVE modelling methods for chopped carbon fiber SMC

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Zhangxing; Li, Yi; Shao, Yimin

To achieve vehicle light-weighting, the chopped carbon fiber sheet molding compound (SMC) is identified as a promising material to replace metals. However, there are no effective tools and methods to predict the mechanical property of the chopped carbon fiber SMC due to the high complexity in microstructure features and the anisotropic properties. In this paper, the Representative Volume Element (RVE) approach is used to model the SMC microstructure. Two modeling methods, the Voronoi diagram-based method and the chip packing method, are developed for material RVE property prediction. The two methods are compared in terms of the predicted elastic modulus andmore » the predicted results are validated using the Digital Image Correlation (DIC) tensile test results. Furthermore, the advantages and shortcomings of these two methods are discussed in terms of the required input information and the convenience of use in the integrated processing-microstructure-property analysis.« less

Combined Analysis of the Binary Lens Caustic-crossing Event MACHO 98-SMC-1

NASA Astrophysics Data System (ADS)

Afonso, C.; Alard, C.; Albert, J. N.; Andersen, J.; Ansari, R.; Aubourg, É.; Bareyre, P.; Bauer, F.; Beaulieu, J. P.; Bouquet, A.; Char, S.; Charlot, X.; Couchot, F.; Coutures, C.; Derue, F.; Ferlet, R.; Glicenstein, J. F.; Goldman, B.; Gould, A.; Graff, D.; Gros, M.; Haissinski, J.; Hamilton, J. C.; Hardin, D.; de Kat, J.; Kim, A.; Lasserre, T.; Lesquoy, É.; Loup, C.; Magneville, C.; Marquette, J. B.; Maurice, É.; Milsztajn, A.; Moniez, M.; Palanque-Delabrouille, N.; Perdereau, O.; Prévot, L.; Regnault, N.; Rich, J.; Spiro, M.; Vidal-Madjar, A.; Vigroux, L.; Zylberajch, S.; Alcock, C.; Allsman, R. A.; Alves, D.; Axelrod, T. S.; Becker, A. C.; Cook, K. H.; Drake, A. J.; Freeman, K. C.; Griest, K.; King, L. J.; Lehner, M. J.; Marshall, S. L.; Minniti, D.; Peterson, B. A.; Pratt, M. R.; Quinn, P. J.; Rodgers, A. W.; Stetson, P. B.; Stubbs, C. W.; Sutherland, W.; Tomaney, A.; Vandehei, T.; Rhie, S. H.; Bennett, D. P.; Fragile, P. C.; Johnson, B. R.; Quinn, J.; Udalski, A.; Kubiak, M.; Szymański, M.; Pietrzyński, G.; Woźniak, P.; Zebruń, K.; Albrow, M. D.; Caldwell, J. A. R.; DePoy, D. L.; Dominik, M.; Gaudi, B. S.; Greenhill, J.; Hill, K.; Kane, S.; Martin, R.; Menzies, J.; Naber, R. M.; Pogge, R. W.; Pollard, K. R.; Sackett, P. D.; Sahu, K. C.; Vermaak, P.; Watson, R.; Williams, A.

2000-03-01

We fit the data for the binary lens microlensing event MACHO 98-SMC-1 from five different microlensing collaborations and find two distinct solutions characterized by binary separation d and mass ratio q: (d,q)=(0.54,0.50) and (d,q)=(3.65,0.36), where d is in units of the Einstein radius. However, the relative proper motion of the lens is very similar in the two solutions, 1.30 km s-1 kpc-1 and 1.48 km s-1 kpc-1, thus confirming that the lens is in the Small Magellanic Cloud. The close binary can be either rotating or approximately static but the wide binary must be rotating at close to its maximum allowed rate to be consistent with all the data. We measure limb-darkening coefficients for five bands ranging from I to V. As expected, these progressively decrease with rising wavelength. This is the first measurement of limb darkening for a metal-poor A star.

The young SMC cluster NGC 330

NASA Technical Reports Server (NTRS)

Carney, B. W.; Janes, K. A.; Flower, P. J.

1985-01-01

A color-magnitude diagram has been obtained for the young SMC cluster NGC 330. The diagram shows a well-defined main sequence, a group of blue supergiants, a group of red supergiants between B-V = 1.2 m and 1.6 m about one magnitude fainter, and an empty Hertzsprung gap. The surrounding field is a composite of a very gold population resembling galactic globular clusters and a very young population. DDO and infrared photometry strongly suggest that the cluster is metal-poor, but a definitive measure could not be made because of calibration difficulties. The cluster's age is estimated at 12 million years, with the surrounding field about 50 percent older. The cluster will prove very useful in testing stellar evolution models for young, metal-poor stars if the cluster's metallicity can be established via high-resolution spectroscopy.

Gene-by-social-environment interaction (GxSE) between ADCYAP1R1 genotype and neighborhood crime predicts major depression symptoms in trauma-exposed women.

PubMed

Lowe, Sarah R; Pothen, John; Quinn, James W; Rundle, Andrew; Bradley, Bekh; Galea, Sandro; Ressler, Kerry J; Koenen, Karestan C

2015-11-15

Few studies have explored interactions between genes and social environmental exposures (GxSEs) for trauma-related psychopathology, including symptoms of posttraumatic stress (PTS) and major depression (MD). The extant literature suggests the possibility of a GxSE between the rs2267735 variant of the ADCYAP1R1 gene and neighborhood crime. The current study aimed to explore this possibility among a predominantly African American sample of trauma-exposed women. Female participants (N=1361) were recruited from a public hospital, and completed measures of PTS and MD symptoms and provided DNA samples. Participants' home addresses were mapped onto 300 neighborhoods (2010 census tracts), and data on crime within neighborhoods was collected. Multilevel models detected a significant GxSE between rs2267735 and neighborhood crime for MD symptoms (p=.01). Having two copies of the risk (C) allele was associated with higher MD symptoms for participants living in high-crime neighborhoods. At least six limitations are noteworthy: (1) low statistical power; (2) use of self-report symptom inventories; (3) lack of information on symptom onset; (4) homogeneous sample from a single metropolitan area; (5) non-specific index of crime; and (6) use of census tracts to define neighborhoods. The results provide further evidence of GxSEs for psychiatric outcomes among trauma-exposed populations. Further investigations of genetic factors for trauma-related psychopathology should include careful assessments of the social environment. Copyright © 2015 Elsevier B.V. All rights reserved.

Gene-by-social-environment interaction (GxSE) between ADCYAP1R1 genotype and neighborhood crime predicts major depression symptoms in trauma-exposed women

PubMed Central

Lowe, Sarah R.; Pothen, John; Quinn, James W.; Rundle, Andrew; Bradley, Bekh; Galea, Sandro; Ressler, Kerry J.; Koenen, Karestan C.

2015-01-01

Background Few studies have explored interactions between genes and social environmental exposures (GxSEs) for trauma-related psychopathology, including symptoms of posttraumatic stress (PTS) and major depression (MD). The extant literature suggests the possibility of a GxSE between the rs2267735 variant of the ADCYAP1R1 gene and neighborhood crime. The current study aimed to explore this possibility among a predominantly African American sample of trauma-exposed women. Methods Female participants (N = 1361) were recruited from a public hospital, and completed measures of PTS and MD symptoms and provided DNA samples. Participants’ home addresses were mapped onto 300 neighborhoods (2010 census tracts), and data on crime within neighborhoods was collected. Results Multilevel models detected a significant GxSE between rs2267735 and neighborhood crime for MD symptoms (p = .01). Having two copies of the risk (C) allele was associated with higher MD symptoms for participants living in high-crime neighborhoods. Limitations At least six limitations are noteworthy: (1) low statistical power; (2) use of self-report symptom inventories; (3) lack of information on symptom onset; (4) homogeneous sample from a single metropolitan area; (5) non-specific index of crime; and (6) use of census tracts to define neighborhoods. Conclusions The results provide further evidence of GxSEs for psychiatric outcomes among trauma-exposed populations. Further investigations of genetic factors for trauma-related psychopathology should include careful assessments of the social environment. PMID:26334183

On the Origin of the Near-infrared Emission from the Neutron-star Low-mass X-Ray Binary GX 9+1

NASA Astrophysics Data System (ADS)

van den Berg, Maureen; Homan, Jeroen

2017-01-01

We have determined an improved position for the luminous persistent neutron-star low-mass X-ray binary and atoll source GX 9+1 from archival Chandra X-ray Observatory data. The new position significantly differs from a previously published Chandra position for this source. Based on the revised X-ray position we have identified a new near-infrared (NIR) counterpart to GX 9+1 in Ks-band images obtained with the PANIC and FourStar cameras on the Magellan Baade Telescope. NIR spectra of this {K}s=16.5+/- 0.1 mag star, taken with the FIRE spectrograph on the Baade Telescope, show a strong Br γ emission line, which is a clear signature that we discovered the true NIR counterpart to GX 9+1. The mass donor in GX 9+1 cannot be a late-type giant, as such a star would be brighter than the estimated absolute Ks magnitude of the NIR counterpart. The slope of the dereddened NIR spectrum is poorly constrained due to uncertainties in the column density NH and NIR extinction. Considering the source’s distance and X-ray luminosity, we argue that NH likely lies near the high end of the previously suggested range. If this is indeed the case, the NIR spectrum is consistent with thermal emission from a heated accretion disk, possibly with a contribution from the secondary. In this respect, GX 9+1 is similar to other bright atolls and the Z sources, whose NIR spectra do not show the slope that is expected for a dominant contribution from optically thin synchrotron emission from the inner regions of a jet. This paper includes data gathered with the 6.5 m Magellan Telescopes located at Las Campanas Observatory, Chile.

Introducing cellulose nanocrystals in sheet molding compounds (SMC)

Treesearch

Amir Asadi; Mark Miller; Sanzida Sultana; Robert J. Moon; Kyriaki Kalaitzidou

2016-01-01

The mechanical properties of short glass fiber/epoxy composites containing cellulose nanocrystals (CNC) made using sheet molding compound (SMC) manufacturing method as well as the rheological and thermomechanical properties of the CNC-epoxy composites were investigated as a function of the CNC content. CNC up to 1.4 wt% were dispersed in the epoxy to produce the resin...

Identification of novel eubacteria from spent mushroom compost (SMC) waste by DNA sequence typing: ecological considerations of disposal on agricultural land.

PubMed

Watabe, M; Rao, J R; Xu, J; Millar, B C; Ward, R F; Moore, J E

2004-01-01

A small study was undertaken to examine the microbiological characteristics of spent mushroom compost (SMC), which is the major waste by-product of the mushroom industry and which is regularly disposed off by application to agricultural land. The primary aim of this study was to examine SMC for the presence of faecal bacterial pathogens, including Campylobacter spp., Salmonella spp. and Listeria monocytogenes. Secondly it was desirable to quantify bacterial and fungal populations within SMC, and also qualitatively identify the diversity of bacterial populations within SMC, through employment of rDNA PCR and direct sequencing techniques on the culturable microflora. Conventional microbiological analyses of SMC material (n=30) from six commercial operations in both Northern Ireland and the Republic of Ireland, failed to detect Salmonella spp, Listeria spp. or Campylobacter spp. in any of the SMC material examined. Total aerobic plate counts gave a mean count of log10 7.01 colony forming units (cfu) per gram SMC material (range: log10 6.53-7.52 cfu/g). Fungal counts gave a mean count of log(10) 4.57 cfu per gram SMC material (range: log10 3.93-4.98 cfu/g). From a total of greater than 50 colony picks, a total of 12 bacterial morphotypes were identified and were further examined by employment of partial 16S rRNA gene amplification and sequencing techniques, yielding several genera and species, including Bacillus licheniformis, Bacillus subtilis, Klebsiella/Enterobacter sp. Microbacterium sp. Paenibacillus lentimorbus, Pseudomonas mevalonii, Sphingobacterium multivorum and Stenotrophomonas sp. This is the first preliminary report on the microbial diversity of SMC waste and demonstrates the presence of several species that have not been previously described in SMC, in addition to two potentially novel species within the genera Microbacterium and Stenotrophomonas. It is thereby important to examine the ecological microbe-microbe and plant-microbe interactions that are

Sub-second optical flaring in GX 339-4 during the 2017 outburst early rise

NASA Astrophysics Data System (ADS)

Gandhi, P.; Kotze, M. M.; Buckley, D. A. H.; Paice, J. A.; Altamirano, D.; Charles, P. A.; Russell, D. M.; Fabian, A. C.

2017-10-01

The black hole X-ray binary GX 339-4 has been caught at the early stage of a new outburst. According to optical monitoring, the outburst began between 2017-08-24 and 2017-09-14, presumably in the outer disc (ATel #10797).

VizieR Online Data Catalog: XMM-Newton point-source catalogue of the SMC (Sturm+, 2013)

NASA Astrophysics Data System (ADS)

Sturm, R.; Haberl, F.; Pietsch, W.; Ballet, J.; Hatzidimitriou, D.; Buckley, D. A. H.; Coe, M.; Ehle, M.; Filipovic, M. D.; La Palombara, N.; Tiengo, A.

2013-07-01

The XMM-Newton survey of the Small Magellanic Cloud (SMC) yields a complete coverage of the bar and eastern wing in the 0.2-12.0keV band. This catalogue comprises 3053 unique X-ray point sources and sources with moderate extent that have been reduced from 5236 individual detections found in observations between April 2000 and April 2010. Sources have a median position uncertainty of 1.3" (1σ) and limiting fluxes down to ~1*10-14erg/s/cm2 in the 0.2-4.5keV band, corresponding to 5*1033erg/s for sources in the SMC. Sources have been classified using hardness ratios, X-ray variability, and their multi-wavelength properties. In addition to the main-field (5.58deg2) available outer fields have been included in the catalogue, yielding a total field area of 6.32deg2. X-ray sources with high extent (>40", e.g. supernova remnants and galaxy cluster) have been presented by Haberl et al. (2012, Cat. J/A+A/545/A128) (2 data files).

Master Console, SMC

NASA Technical Reports Server (NTRS)

Paga Marrero, Hector Jose

2013-01-01

The Master Console oversees the function of Computer Systems in Firing room 1 (FR1). Master Console Operators, MCOs' for short, are our customer. I was integrated into the System Monitoring and Control (SMC) software team that is under the guidance of David Slaiman, who is the product group lead. I have been brought up to speed with System Monitoring and Control. The initial time spent reading SMC software design description and understanding how it works. The current Firing Room 1 Console Display is a floor layout giving the MCO two essential pieces of information which are Health and Status. When an issue arises, the MCO has to look on the display to find which console is affected and then the MCO must use the Reference designator from the display to manually search for the Portal Workstation (PWS) installed in the console using the hardware map; which is a long process to lookup a PWS if an issue is present. My project is to make the FR1 Console Display easier for the MCO's to pinpoint PWS's without having to lookup additional resources in the process. My project also includes updating Firing Room 1 Console Display to include the F1R Non-Redundant Set. The display does not have good use of space and functionality. PWS numbers were not present in the previous design and are the critical component in efficient understanding and administration of the consoles. Part of the process includes getting feedback from the customer, instead of just emailing them with a question, we made a proposal with changes so they could respond and give us their input; which proved to be an effective method for engaging them. In order to do this I had to use the Display Editor (DE) tool developed by NASA, Paint.Net and Visio. The process I have been using has been Visio to alter the floor layout of Firing Room and take advantage of the white areas, and then I take the altered floor plan into Paint.Net. Once in Paint.Net I put the new floor plan as a background to the standard console

The Fur-Iron Complex Modulates Expression of the Quorum-Sensing Master Regulator, SmcR, To Control Expression of Virulence Factors in Vibrio vulnificus

PubMed Central

Kim, In Hwang; Wen, Yancheng; Son, Jee-Soo; Lee, Kyu-Ho

2013-01-01

The gene vvpE, encoding the virulence factor elastase, is a member of the quorum-sensing regulon in Vibrio vulnificus and displays enhanced expression at high cell density. We observed that this gene was repressed under iron-rich conditions and that the repression was due to a Fur (ferric uptake regulator)-dependent repression of smcR, a gene encoding a quorum-sensing master regulator with similarity to luxR in Vibrio harveyi. A gel mobility shift assay and a footprinting experiment demonstrated that the Fur-iron complex binds directly to two regions upstream of smcR (−82 to −36 and −2 to +27, with respect to the transcription start site) with differing affinities. However, binding of the Fur-iron complex is reversible enough to allow expression of smcR to be induced by quorum sensing at high cell density under iron-rich conditions. Under iron-limiting conditions, Fur fails to bind either region and the expression of smcR is regulated solely by quorum sensing. These results suggest that two biologically important environmental signals, iron and quorum sensing, converge to direct the expression of smcR, which then coordinates the expression of virulence factors. PMID:23716618

PharmacoGx: an R package for analysis of large pharmacogenomic datasets.

PubMed

Smirnov, Petr; Safikhani, Zhaleh; El-Hachem, Nehme; Wang, Dong; She, Adrian; Olsen, Catharina; Freeman, Mark; Selby, Heather; Gendoo, Deena M A; Grossmann, Patrick; Beck, Andrew H; Aerts, Hugo J W L; Lupien, Mathieu; Goldenberg, Anna; Haibe-Kains, Benjamin

2016-04-15

Pharmacogenomics holds great promise for the development of biomarkers of drug response and the design of new therapeutic options, which are key challenges in precision medicine. However, such data are scattered and lack standards for efficient access and analysis, consequently preventing the realization of the full potential of pharmacogenomics. To address these issues, we implemented PharmacoGx, an easy-to-use, open source package for integrative analysis of multiple pharmacogenomic datasets. We demonstrate the utility of our package in comparing large drug sensitivity datasets, such as the Genomics of Drug Sensitivity in Cancer and the Cancer Cell Line Encyclopedia. Moreover, we show how to use our package to easily perform Connectivity Map analysis. With increasing availability of drug-related data, our package will open new avenues of research for meta-analysis of pharmacogenomic data. PharmacoGx is implemented in R and can be easily installed on any system. The package is available from CRAN and its source code is available from GitHub. bhaibeka@uhnresearch.ca or benjamin.haibe.kains@utoronto.ca Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

[Expression and characterization of a neutral Enterobacter cloacae GX-3 invertase].

PubMed

Zhao, Yingli; Wu, Qianqian; Zhang, Zhikai; Wang, Zilong; Wei, Yutuo; Huang, Ribo; Du, Liqin

2015-04-04

To characterize a neutral invertase from Enterobacter cloacae GX-3. By searching GenBank database, we found the genes encoding invertase from the same genus Enterobacter. These sequences were aligned and analyzed. Then, a gene encoding neutral invertase was amplified by PCR. The recombinant plasmid pQE-Einv was constructed. We purified the expressed protein Einv with nickel-nitrilotriacetic acid chromatography. At last, the characterics of the recombinant protein Einv were studied in detail. A gene encoding neutral invertase was discovered and cloned from E. cloacae GX-3. The recombinant enzyme Einv was characterized. Einv had an optimum pH of 6.5 and an optimum temperature of 40 degrees C. The results of sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE) and gel permeation chromatography ( GPC) showed that Einv was a homo-dimer protein. Einv retained 80% activity at sucrose concentrations up to 1170 mmol/L. But, Einv had no transglycosylation activity at high sucrose concentration. It could hydrolyze raffinose, 1-kestose, nystose, fructofuranosylnystose and stachyose. It is first reported that an invertase from Enterobacter cloacae is a beta-fructofuranosidase at neutral pH range. It only has hydrolysis activity without tranglycosylation activity. These characteristics indicate that the neutral invertase Einv has important applications in food industry.

A de novo sSMC(22) Characterized by High-Resolution Arrays in a Girl with Cat-Eye Syndrome without Coloboma.

PubMed

Córdova-Fletes, C; Domínguez, M G; Vázquez-Cárdenas, A; Figuera, L E; Neira, V A; Rojas-Martínez, A; Ortiz-López, R

2012-09-01

Cat-eye syndrome (CES) results from trisomy or tetrasomy of proximal 22q originated by a small supernumerary marker chromosome (sSMC). Two critical regions for the major clinical features of CES (CESCRs) have been suggested; however, CES clinical presentation often does not correlate with the sSMC genetic content. We report here a CES girl without coloboma and carrier of a de novo type I sSMC(22) as determined by G- and C-banding, NOR staining and microarrays. This sSMC included 6 distal genes outside the original CESCR and led to a tetrasomy for 22q11.1-22q11.21. The patient's final karyotype was 47,XX,+psu dic(22)(q11.21).arr 22q11.1q11.21(15,250,000-17,035,860)×4 dn. The amplified region outside of CESCR included some genes that may be related to neurologic, heart and renal abnormalities. Conversely, even though the amplification included the CECR2 gene, a major candidate for eye features, there was no coloboma in the patient. The genetic delineation of the present sSMC further strengthens that the CES clinical presentation does not fit completely with the duplicated genetic content and that CES is actually a genomic disorder. Furthermore, since we observed no mosaicism, we believe that other mechanisms might be behind the variability of CES phenotypes as well, mainly those related with functional interactions among amplified genes.

Operational warnings issued by the SMC in the 8th March snow event in Catalonia

NASA Astrophysics Data System (ADS)

Vilaclara, E.; Segalà, S.; Andrés, A.; Aran, M.

2010-09-01

The snowfall event of 8th March 2010 was one of the most important of the last years in Catalonia, with high societal impact in the communication and in the power distribution. Since 2002, after an agreement between Meteorological Service of Catalonia (SMC) and Civil Protection authority, the SMC is the agency responsible to carry out meteorological warnings in Catalonia. These warnings are issued depending on the thresholds which are expected to be exceeded (three different probability degrees are defined), and are characterized by a high spatial and temporal resolution. In the snow event of 8th March, forecasting team of the SMC did the first meteorological warning three days before, on 5th March. During the two following days broadcasted four different warnings, taking into account the high probability of exceeding 2 cm of snow above 200 meters, 15 above 400 m and 30 above 800 m. Furthermore, the SMC disseminated also two special press releases with the aim to extend the forecast to the public. In the afternoon of Sunday, 7th March, the snow precipitation started in some areas of Catalonia. From this moment, the SMC followed the surveillance of situation with the remote sensing tools and the Meteorological Observers Network data. This network is formed by a hundred of spotters with mobile phones able to transmit observations in real time to our web site. This surveillance and the conceptual model of snow events in Catalonia allowed the forecasters to improve the estimation of the snow level forecasted by mesoscale meteorological models. In this event, the snow level obtained from these models was higher than the real one. In spite of the accuracy of the forecast, the impact was very important in Catalonia. On one hand, it was due to the exceptionality of the event, with 3 cm of snow in Barcelona in the afternoon of a working day. On the other hand, the large amount of wet snow precipitation (until 100 mm), and the wind, contributed to an important snow

A deep survey of the X-ray binary populations in the SMC

NASA Astrophysics Data System (ADS)

Zezas, A.; Antoniou, V.

2017-10-01

The Small Magellanic Cloud (SMC) has been the subject of systematic X-ray surveys over the past two decades, which have yielded a rich population of high-mass X-ray binaries consisting predominantly of Be/X-ray binaries. We present results from our deep Chandra survey of the SMC which targeted regions with stellar populations ranging between ˜10-100 Myr. X-ray luminosities down to ˜3×10^{32} erg/s were reached, probing all active accreting binaries and extending well into the regime of quiescent accreting binaries and X-ray emitting normal stars. We measure the dependence of the formation efficiency of X-ray binaries on age. We also detect pulsations from 19 known and one new candidate pulsar. We construct the X-ray luminosity function in different regions of the SMC, which shows clear evidence for the propeller effect the centrifugal inhibition of accretion due to the interaction of the accretion flow with the pulsar's magnetic field. Finally we compare these results with predictions for the formation efficiency of X-ray binaries as a function of age from X-ray binary population synthesis models.

GX 3+1: The Stability of Spectral Index as a Function of Mass Accretion Rate

NASA Technical Reports Server (NTRS)

Seifana, Elena; Titarchuk, Lev

2012-01-01

We present an analysis of the spectral and timing properties observed in X-rays from neutron star (NS) binary GX 3+1 (4U 1744-26) during long-term transitions between the faint and bright phases superimposed on short-term transitions between lower banana (LB) and upper banana (UB) branches in terms of its color-color diagram, We analyze all observations of this source obtained with the Rossi X-ray Timing Explorer and BeppoSAX satellites, We find that the X-ray broadband energy spectra during these spectral transitions can be adequately reproduced by a composition of a low-temperature blackbody component, a Comptonized component (COMPTB), and Gaussian component We argue that the electron temperature kTe of the Compton cloud monotonically increases from 2.3 keY to 4.5 keY, when GX 3+1 makes a transition from UB to LB. We also detect an evolution of noise components (a very low frequency noise and a high-frequency noise) during these LB-UB transitions. Using a disk seed photon normalization of COMPTB, which is proportional to the mass accretion rate, we find that the photon power-law index Gamma is almost constant (Gamma = 2.00 +/- 0.02) when mass accretion rate changes by factor four. In addition, we find that the emergent spectrum is dominated by the strong Comptonized component We interpret this quasi-stability of the index Gamma and a particular form of the spectrum in the framework of a model in which the energy release in the transition layer located between the accretion disk and NS surface dominates that in the disk. Moreover, this index stability effect now established for GX 3+ I was previously found in the atoll source 4U 1728-34 and suggested for a number of other low-mass X-ray NS binaries. This intrinsic behavior of NSs, in particular for atoll sources, is fundamentally different from that seen in black hole binary sources where the index monotonically increases during spectral transition from the low state to the high state and then finally saturates at

GX 3+1: The Stability of Spectral Index as a Function of Mass Accretion Rate

NASA Astrophysics Data System (ADS)

Seifina, Elena; Titarchuk, Lev

2012-03-01

We present an analysis of the spectral and timing properties observed in X-rays from neutron star (NS) binary GX 3+1 (4U 1744-26) during long-term transitions between the faint and bright phases superimposed on short-term transitions between lower banana (LB) and upper banana (UB) branches in terms of its color-color diagram. We analyze all observations of this source obtained with the Rossi X-ray Timing Explorer and Beppo SAX satellites. We find that the X-ray broadband energy spectra during these spectral transitions can be adequately reproduced by a composition of a low-temperature blackbody component, a Comptonized component (COMPTB), and a Gaussian component. We argue that the electron temperature kTe of the Compton cloud monotonically increases from 2.3 keV to 4.5 keV, when GX 3+1 makes a transition from UB to LB. We also detect an evolution of noise components (a very low frequency noise and a high-frequency noise) during these LB-UB transitions. Using a disk seed photon normalization of COMPTB, which is proportional to the mass accretion rate, we find that the photon power-law index Γ is almost constant (Γ = 2.00 ± 0.02) when mass accretion rate changes by a factor of four. In addition, we find that the emergent spectrum is dominated by the strong Comptonized component. We interpret this quasi-stability of the index Γ and a particular form of the spectrum in the framework of a model in which the energy release in the transition layer located between the accretion disk and NS surface dominates that in the disk. Moreover, this index stability effect now established for GX 3+1 was previously found in the atoll source 4U 1728-34 and suggested for a number of other low-mass X-ray NS binaries (see Farinelli & Titarchuk). This intrinsic behavior of NSs, in particular for atoll sources, is fundamentally different from that seen in black hole binary sources where the index monotonically increases during spectral transition from the low state to the high state

GX 3+1: THE STABILITY OF SPECTRAL INDEX AS A FUNCTION OF MASS ACCRETION RATE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Seifina, Elena; Titarchuk, Lev, E-mail: seif@sai.msu.ru, E-mail: titarchuk@fe.infn.it, E-mail: lev@milkyway.gsfc.nasa.gov

2012-03-10

We present an analysis of the spectral and timing properties observed in X-rays from neutron star (NS) binary GX 3+1 (4U 1744-26) during long-term transitions between the faint and bright phases superimposed on short-term transitions between lower banana (LB) and upper banana (UB) branches in terms of its color-color diagram. We analyze all observations of this source obtained with the Rossi X-ray Timing Explorer and Beppo SAX satellites. We find that the X-ray broadband energy spectra during these spectral transitions can be adequately reproduced by a composition of a low-temperature blackbody component, a Comptonized component (COMPTB), and a Gaussian component.more » We argue that the electron temperature kT{sub e} of the Compton cloud monotonically increases from 2.3 keV to 4.5 keV, when GX 3+1 makes a transition from UB to LB. We also detect an evolution of noise components (a very low frequency noise and a high-frequency noise) during these LB-UB transitions. Using a disk seed photon normalization of COMPTB, which is proportional to the mass accretion rate, we find that the photon power-law index {Gamma} is almost constant ({Gamma} = 2.00 {+-} 0.02) when mass accretion rate changes by a factor of four. In addition, we find that the emergent spectrum is dominated by the strong Comptonized component. We interpret this quasi-stability of the index {Gamma} and a particular form of the spectrum in the framework of a model in which the energy release in the transition layer located between the accretion disk and NS surface dominates that in the disk. Moreover, this index stability effect now established for GX 3+1 was previously found in the atoll source 4U 1728-34 and suggested for a number of other low-mass X-ray NS binaries (see Farinelli and Titarchuk). This intrinsic behavior of NSs, in particular for atoll sources, is fundamentally different from that seen in black hole binary sources where the index monotonically increases during spectral transition from

Coherent variability of GX 1+4

NASA Astrophysics Data System (ADS)

Nielsen, Ann-Sofie Bak; Patruno, Alessandro

2018-06-01

The accreting pulsar GX 1+4 is a symbiotic X-ray binary system with a M-type giant star companion. The system has a spin period of about 150 s and a proposed strong magnetic field of 1012-1014G. In this paper we study the coherent variability of the source and attempt to find a phase-coherent solution for the pulsar. We also test for the presence of a pulse phase - flux correlation, similar to what is observed for the accreting millisecond X-ray pulsars, in order to test whether this feature is dependent on the magnetic field strength. We find that no phase coherent solution exists which suggests that the pulsar is accreting plasma from a wind rather than an accretion disc. We also find evidence that the pulse phase is not correlated with the X-ray flux, which strengthens the idea that such relation might be present only in weak magnetic field sources like accreting millisecond pulsars.

X-Ray Dust Scattering At Small Angles: The Complete Halo Around GX13+1

NASA Technical Reports Server (NTRS)

Smith, Randall K.

2007-01-01

The exquisite angular resolution available with Chandra should allow precision measurements of faint diffuse emission surrounding bright sources, such as the X-ray scattering halos created by interstellar dust. However, the ACIS CCDs suffer from pileup when observing bright sources, and this creates difficulties when trying to extract the scattered halo near the source. The initial study of the X-ray halo around GX13+1 using only the ACIS-I detector done by Smith, Edgar & Shafer (2002) suffered from a lack of sensitivity within 50" of the source, limiting what conclusions could be drawn. To address this problem, observations of GX13+1 were obtained with the Chandra HRC-I and simultaneously with the RXTE PCA. Combined with the existing ACIS-I data, this allowed measurements of the X-ray halo between 2-1000". After considering a range of dust models, each assumed to be smoothly distributed with or without a dense cloud along the line of sight, the results show that there is no evidence in this data for a dense cloud near the source, as suggested by Xiang et al. (2005). In addition, although no model leads to formally acceptable results, the Weingartner & Draine (2001) and all but one of the composite grain models from Zubko, Dwek & Arendt (2004) give particularly poor fits.

Smc5/6-Mms21 Prevents and Eliminates Inappropriate Recombination Intermediates in Meiosis

PubMed Central

Xaver, Martin; Huang, Lingzhi; Chen, Doris; Klein, Franz

2013-01-01

Repairing broken chromosomes via joint molecule (JM) intermediates is hazardous and therefore strictly controlled in most organisms. Also in budding yeast meiosis, where production of enough crossovers via JMs is imperative, only a subset of DNA breaks are repaired via JMs, closely regulated by the ZMM pathway. The other breaks are repaired to non-crossovers, avoiding JM formation, through pathways that require the BLM/Sgs1 helicase. “Rogue” JMs that escape the ZMM pathway and BLM/Sgs1 are eliminated before metaphase by resolvases like Mus81-Mms4 to prevent chromosome nondisjunction. Here, we report the requirement of Smc5/6-Mms21 for antagonizing rogue JMs via two mechanisms; destabilizing early intermediates and resolving JMs. Elimination of the Mms21 SUMO E3-ligase domain leads to transient JM accumulation, depending on Mus81-Mms4 for resolution. Absence of Smc6 leads to persistent rogue JMs accumulation, preventing chromatin separation. We propose that the Smc5/6-Mms21 complex antagonizes toxic JMs by coordinating helicases and resolvases at D-Loops and HJs, respectively. PMID:24385936

Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3–Scc1 gate

PubMed Central

Buheitel, Johannes; Stemmann, Olaf

2013-01-01

Faithful transmission of chromosomes during eukaryotic cell division requires sister chromatids to be paired from their generation in S phase until their separation in M phase. Cohesion is mediated by the cohesin complex, whose Smc1, Smc3 and Scc1 subunits form a tripartite ring that entraps both DNA double strands. Whereas centromeric cohesin is removed in late metaphase by Scc1 cleavage, metazoan cohesin at chromosome arms is displaced already in prophase by proteolysis-independent signalling. Which of the three gates is triggered by the prophase pathway to open has remained enigmatic. Here, we show that displacement of human cohesin from early mitotic chromosomes requires dissociation of Smc3 from Scc1 but no opening of the other two gates. In contrast, loading of human cohesin onto chromatin in telophase occurs through the Smc1–Smc3 hinge. We propose that the use of differently regulated gates for loading and release facilitates unidirectionality of DNA's entry into and exit from the cohesin ring. PMID:23361318

IKT 16: the first X-ray confirmed composite SNR in the SMC

NASA Astrophysics Data System (ADS)

Maitra, C.; Ballet, J.; Filipović, M. D.; Haberl, F.; Tiengo, A.; Grieve, K.; Roper, Q.

2015-12-01

Aims: IKT 16 is an X-ray and radio-faint supernova remnant (SNR) in the Small Magellanic Cloud (SMC). A detailed X-ray study of this SNR with XMM-Newton confirmed the presence of a hard X-ray source near its centre, indicating the detection of the first composite SNR in the SMC. With a dedicated Chandra observation we aim to resolve the point source and confirm its nature. We also acquire new ATCA observations of the source at 2.1 GHz with improved flux density estimates and resolution. Methods: We perform detailed spatial and spectral analysis of the source. With the highest resolution X-ray and radio image of the centre of the SNR available today, we resolve the source and confirm its pulsar wind nebula (PWN) nature. Further, we constrain the geometrical parameters of the PWN and perform spectral analysis for the point source and the PWN separately. We also test for the radial variations of the PWN spectrum and its possible east west asymmetry. Results: The X-ray source at the centre of IKT 16 can be resolved into a symmetrical elongated feature centring a point source, the putative pulsar. Spatial modelling indicates an extent of 5.2'' of the feature with its axis inclined at 82° east from north, aligned with a larger radio feature consisting of two lobes almost symmetrical about the X-ray source. The picture is consistent with a PWN which has not yet collided with the reverse shock. The point source is about three times brighter than the PWN and has a hard spectrum of spectral index 1.1 compared to a value 2.2 for the PWN. This points to the presence of a pulsar dominated by non-thermal emission. The expected Ė is ~1037 erg s-1 and spin period <100 ms. However, the presence of a compact nebula unresolved by Chandra at the distance of the SMC cannot completely be ruled out. The reduced images (FITS files) are only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/584/A

Optical photometry and polarimetry of GX 339-4 during its outburst rise

NASA Astrophysics Data System (ADS)

Russell, D. M.; Lewis, F.; Casella, P.; Pretorius, M. L.; Fender, R. P.; Roche, P.; Clark, S.

2009-03-01

GX 339-4 is currently brightening at X-ray, UV, optical and radio frequencies (ATel #1945, #1954, #1960). Our monitoring campaign with the Faulkes Telescope South (ATel #1586) gives the following recent magnitudes (light curves are below; errors are ~ 0.02 mag): 2009-02-20 (MJD 54882.65): i' ~ 16.66 (and varying by ~ 0.2 mag in 10 minutes) 2009-02-21 (MJD 54883.76): V ~ 16.94, R ~ 16.46, i' ~ 16.68 2009-03-06 (MJD 54896.76): V ~ 16.37, R ~ 15.94, i' ~ 15.50 2009-03-07 (MJD 54897.76): V ~ 16.31, R ~ 15.89, i' ~ 15.65 The source brightened by 0.6, 0.5 and 1.2 mag in V, R and i' respectively in 13 days between 2009-02-21 and 2009-03-06.

[Tissue repositories for research at Sheba Medical Center(SMC].

PubMed

Cohen, Yehudit; Barshack, Iris; Onn, Amir

2013-06-01

Cancer is the number one cause of death in both genders. Breakthroughs in the understanding of cancer biology, the identification of prognostic factors, and the development of new treatments are increasingly dependent on access to human cancer tissues with linked clinicopathological data. Access to human tumor samples and a large investment in translational research are needed to advance this research. The SMC tissue repositories provide researchers with biological materials, which are essential tools for cancer research. SMC tissue repositories for research aim to collect, document and preserve human biospecimens from patients with cancerous diseases. This is in order to provide the highest quality and well annotated biological biospecimens, used as essential tools to achieve the growing demands of scientific research needs. Such repositories are partners in acceLerating biomedical research and medical product development through clinical resources, in order to apply best options to the patients. Following Institutional Review Board approval and signing an Informed Consent Form, the tumor and tumor-free specimens are coLLected by a designated pathologist at the operating room only when there is a sufficient amount of the tumor, in excess of the routine needs. Blood samples are collected prior to the procedure. Other types of specimens collected include ascites fluid, pleural effusion, tissues for Optimal Cutting Temperature [OCT] and primary culture etc. Demographic, clinical, pathologicaL, and follow-up data are collected in a designated database. SMC has already established several organ or disease-specific tissue repositories within different departments. The foundation of tissue repositories requires the concentrated effort of a multidisciplinary team composed of paramedical, medical and scientific professionals. Research projects using these specimens facilitate the development of 'targeted therapy', accelerate basic research aimed at clarifying molecular

"XMM/RXTE Observations of GX 339-4"

NASA Technical Reports Server (NTRS)

Nowak, M. A.; Corbel, S.; Fender, R.; Wilms, J.; Kuster, M.; Bailyn, C.; Coppi, P.

2005-01-01

In March 2003, we performed two simultaneous XMM/RXTE observations of the black hole candidate GX 339-4. Our goal is to compare these data to our prior simultaneous RXTE/ASCA observations (Nowak, Wilms & Dove, 2002). These observations were carried out in timing mode, as opposed to burst mode, and are more complex to analyze than we expected. Specifically, the data suffered from a number of telemetry dropouts (in fact, the standard archive processing failed on these data, and more than a year passed from the time of the observations before the data was delivered to us). Furthermore, the core of the EPIC PSF suffers slightly from pileup and gain shifts. We continue to work on this data, however, and anticipate publishing it within the next academic year. Here we highlight our ongoing work and outline our plans for publication.

On two new X-ray sources in the SMC and the high luminosities of the Magellanic X-ray sources

NASA Technical Reports Server (NTRS)

Clark, G.; Doxsey, R.; Li, F.; Jernigan, J. G.; Van Paradijs, J.

1978-01-01

The discovery of two new X-ray sources, SMC X-2 and SMC X-3, in the Small Magellanic Cloud is reported. They have hard spectra, and their luminosities in the energy range 2-11 keV are 1.0 and 0.7 by 10 to the 38th power erg/sq cm per sec, respectively. It is shown that the luminosity distribution of the known Magellanic X-ray sources, which are now nine in number, is shifted toward higher luminosities with respect to that of similar sources in the Galaxy, and that the cause of the shift is probably an underabundance of heavy elements in the material accreted by the X-ray sources.

Wagging the Dog, Carting the Horse: Testing and Improving Schools. Summary of Conference Proceedings. Research into Practice Project.

ERIC Educational Resources Information Center

Herman, Joan; And Others

The purpose of the conference, "Wagging the Dog, Carting the Horse: Testing vs. Improving California Schools," was to discuss alternative perspectives on testing and evaluation in education and their role in improving teaching and learning. Four papers were presented: (1) "Using Educational Evaluation for the Improvement of California Schools," by…

OPTICAL AND NEAR-INFRARED MONITORING OF THE BLACK HOLE X-RAY BINARY GX 339-4 DURING 2002-2010

DOE Office of Scientific and Technical Information (OSTI.GOV)

Buxton, Michelle M.; Bailyn, Charles D.; Capelo, Holly L.

We present the optical/infrared (O/IR) light curve of the black hole X-ray binary GX 339-4 collected at the SMARTS 1.3 m telescope from 2002 to 2010. During this time the source has undergone numerous state transitions including hard-to-soft state transitions when we see large changes in the near-IR flux accompanied by modest changes in optical flux, and three rebrightening events in 2003, 2005, and 2007 after GX 339-4 transitioned from the soft state to the hard. All but one outburst show similar behavior in the X-ray hardness-intensity diagram. We show that the O/IR colors follow two distinct tracks that reflectmore » either the hard or soft X-ray state of the source. Thus, either of these two X-ray states can be inferred from O/IR observations alone. From these correlations we have constructed spectral energy distributions of the soft and hard states. During the hard state, the near-IR data have the same spectral slope as simultaneous radio data when GX 339-4 was in a bright optical state, implying that the near-IR is dominated by a non-thermal source, most likely originating from jets. Non-thermal emission dominates the near-IR bands during the hard state at all but the faintest optical states, and the fraction of non-thermal emission increases with increasing optical brightness. The spectral slope of the optical bands indicate that a heated thermal source is present during both the soft and hard X-ray states, even when GX 339-4 is at its faintest optical state. We have conducted a timing analysis of the light curve for the hard and soft states and find no evidence of a characteristic timescale within the range of 4-230 days.« less

The SMC-5/6 Complex and the HIM-6 (BLM) Helicase Synergistically Promote Meiotic Recombination Intermediate Processing and Chromosome Maturation during Caenorhabditis elegans Meiosis

PubMed Central

Hong, Ye; Sonneville, Remi; Agostinho, Ana; Meier, Bettina; Wang, Bin; Blow, J. Julian; Gartner, Anton

2016-01-01

Meiotic recombination is essential for the repair of programmed double strand breaks (DSBs) to generate crossovers (COs) during meiosis. The efficient processing of meiotic recombination intermediates not only needs various resolvases but also requires proper meiotic chromosome structure. The Smc5/6 complex belongs to the structural maintenance of chromosome (SMC) family and is closely related to cohesin and condensin. Although the Smc5/6 complex has been implicated in the processing of recombination intermediates during meiosis, it is not known how Smc5/6 controls meiotic DSB repair. Here, using Caenorhabditis elegans we show that the SMC-5/6 complex acts synergistically with HIM-6, an ortholog of the human Bloom syndrome helicase (BLM) during meiotic recombination. The concerted action of the SMC-5/6 complex and HIM-6 is important for processing recombination intermediates, CO regulation and bivalent maturation. Careful examination of meiotic chromosomal morphology reveals an accumulation of inter-chromosomal bridges in smc-5; him-6 double mutants, leading to compromised chromosome segregation during meiotic cell divisions. Interestingly, we found that the lethality of smc-5; him-6 can be rescued by loss of the conserved BRCA1 ortholog BRC-1. Furthermore, the combined deletion of smc-5 and him-6 leads to an irregular distribution of condensin and to chromosome decondensation defects reminiscent of condensin depletion. Lethality conferred by condensin depletion can also be rescued by BRC-1 depletion. Our results suggest that SMC-5/6 and HIM-6 can synergistically regulate recombination intermediate metabolism and suppress ectopic recombination by controlling chromosome architecture during meiosis. PMID:27010650

The SMC-5/6 Complex and the HIM-6 (BLM) Helicase Synergistically Promote Meiotic Recombination Intermediate Processing and Chromosome Maturation during Caenorhabditis elegans Meiosis.

PubMed

Hong, Ye; Sonneville, Remi; Agostinho, Ana; Meier, Bettina; Wang, Bin; Blow, J Julian; Gartner, Anton

2016-03-01

Meiotic recombination is essential for the repair of programmed double strand breaks (DSBs) to generate crossovers (COs) during meiosis. The efficient processing of meiotic recombination intermediates not only needs various resolvases but also requires proper meiotic chromosome structure. The Smc5/6 complex belongs to the structural maintenance of chromosome (SMC) family and is closely related to cohesin and condensin. Although the Smc5/6 complex has been implicated in the processing of recombination intermediates during meiosis, it is not known how Smc5/6 controls meiotic DSB repair. Here, using Caenorhabditis elegans we show that the SMC-5/6 complex acts synergistically with HIM-6, an ortholog of the human Bloom syndrome helicase (BLM) during meiotic recombination. The concerted action of the SMC-5/6 complex and HIM-6 is important for processing recombination intermediates, CO regulation and bivalent maturation. Careful examination of meiotic chromosomal morphology reveals an accumulation of inter-chromosomal bridges in smc-5; him-6 double mutants, leading to compromised chromosome segregation during meiotic cell divisions. Interestingly, we found that the lethality of smc-5; him-6 can be rescued by loss of the conserved BRCA1 ortholog BRC-1. Furthermore, the combined deletion of smc-5 and him-6 leads to an irregular distribution of condensin and to chromosome decondensation defects reminiscent of condensin depletion. Lethality conferred by condensin depletion can also be rescued by BRC-1 depletion. Our results suggest that SMC-5/6 and HIM-6 can synergistically regulate recombination intermediate metabolism and suppress ectopic recombination by controlling chromosome architecture during meiosis.

Chemorheology of in-mold coating for compression molded SMC applications

NASA Astrophysics Data System (ADS)

Ko, Seunghyun; Straus, Elliott J.; Castro, Jose M.

2015-05-01

In-mold coating (IMC) is applied to compression molded sheet molding compound (SMC) exterior automotive or truck body panels as an environmentally friendly alternative to make the surface conductive for subsequent electrostatic painting operations. The coating is a thermosetting liquid that when injected onto the surface of the part cures and bonds to provide a smooth conductive surface. In order to optimize the IMC process, it is essential to predict the time available for flow, that is the time before the thermosetting reaction starts (inhibition time) as well as the time when the coating has enough structural integrity so that the mold can be opened without damaging the part surface (cure time). To predict both the inhibition time and the cure time, it is critical to study the chemorheology of IMC. In this paper, we study the chemorheology for a typical commercial IMC system, and show its relevance to both the flow and cure time for the IMC stage during SMC compression molding.

SMC X-3 Identified with RXTE 7.78s Pulsar from Archive Chandra Data.

NASA Astrophysics Data System (ADS)

Edge, W. R. T.; Coe, M. J.; Corbet, R. H. D.; Markwardt, C. B.; Laycock, S.

2004-01-01

SMC X-3 (Clark et al. 1978, ApJ, 221, L37) has been identified with a previously detected 7.78s RXTE pulsar by using archive Chandra Data. An examination of Chandra Observation ID 2947, which took place between 2002-07-20 23:03:50 and 2002-07-21 01:46:41 (Zezas et al. astro-ph/0310562, 2003), shows SMC X-3 at R.A. = 00h52m05.7s Decl. = -72d26m05s (equinox 2000) with a 90% confidence radial uncertainty of 0.6 arcsec.

41 CFR 105-54.203-2 - Active charters file.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false Active charters file... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.203-2 Active... active charters. ...

41 CFR 105-54.203-2 - Active charters file.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false Active charters file... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.203-2 Active... active charters. ...

41 CFR 105-54.203-2 - Active charters file.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 41 Public Contracts and Property Management 3 2013-07-01 2013-07-01 false Active charters file... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.203-2 Active... active charters. ...

41 CFR 105-54.203-2 - Active charters file.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Active charters file... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.203-2 Active... active charters. ...

How to Prepare SMC and BMC-like Compounds to Perform Relevant Rheological Experiments?

NASA Astrophysics Data System (ADS)

Guiraud, Olivier; Dumont, Pierre J. J.; Orgéas, Laurent

2013-04-01

The study of the rheology of injected or compression moulded compounds like SMC or BMC is made particularly difficult by the high content and the intricate arrangement of their fibrous reinforcement. For these two types of compounds, inappropriate rheological testing protocols and rheometers are often used, which leads to a very large scatter of the experimental data. This study describes specific sampling and specimen's preparation methods, as well as dedicated rheometry devices to test their rheology. Following the proposed protocols, it is possible to obtain rheological measurements showing low scatter of the recorded stress values: about ±10% for SMC and about ±15% for BMC-like compounds.

Rapid Spin-Up Episodes in the Wind-Fed Accreting Pulsar GX 301-2

NASA Technical Reports Server (NTRS)

Koh, Danny T.; Bildsten, Lars; Chakrabarty, Deepto; Nelson, Robert W.; Prince, Thomas A.; Vaughn, Brian A.; Finger, Mark H.; Wilson, Robert B.; Rubin, Bradley C.

1997-01-01

The accreting pulsar GX 301-2 (P = 680 s) has been observed continuously by the large-area detectors of the Burst and Transient Source Experiment (BATSE) instrument on the Compton Gamma Ray Observatory since 1991 April 5. Orbital parameters determined from these data are consistent with previous measurements, with improved accuracy in the current orbital epoch. The most striking features in the pulsar frequency history are two steady and rapid spin-up episodes, with a dot-nu approximately equal to (3_5) x 10(exp -12) Hz/s, each lasting for about 30 days. They probably represent the formation of transient accretion disks in this wind-fed pulsar. Except for these spin-up episodes, there are virtually no net changes in the neutron star spin frequency on long timescales. We suggest that the long-term spin-up trend observed since 1984 (dot-nu is approximately equals 2x10(exp -13) Hz/s) may be due entirely to brief (approximately 20 days) spin-up episodes similar to those we have discovered. We assess different accretion models and their ability to explain the orbital phase dependence of the observed flux. In addition to the previously observed preperiastron peak at orbital phase 0.956 +/- 0.022, we also find a smaller peak close to - at orbital phase 0.498 +/- 0.057. We show that if the companion star's effective temperature is less than 22,000 K, then it must have a mass M(sub c) < 70 solar mass and a radius R(sub c) < 85 solar radius so as not to overfill the tidal lobe at periastron. In order not to overflow the Roche lobe at periastron, the corresponding values are M(sub c) < 55 solar mass and R(sub c) < 68 solar radius. These constraints are nearly at odds with the reclassification of the companion as a B1 Ia + hypergiant.

Evidence of Black Hole Spin in GX 339-4: XMM-Newton/EPIC-pn and RXTE Spectroscopy of the Very High State

NASA Astrophysics Data System (ADS)

Miller, J. M.; Fabian, A. C.; Reynolds, C. S.; Nowak, M. A.; Homan, J.; Freyberg, M. J.; Ehle, M.; Belloni, T.; Wijnands, R.; van der Klis, M.; Charles, P. A.; Lewin, W. H. G.

2004-05-01

We have analyzed spectra of the Galactic black hole GX 339-4 obtained through simultaneous 76 ks XMM-Newton/EPIC-pn and 10 ks Rossi X-Ray Timing Explorer observations during a bright phase of its 2002-2003 outburst. An extremely skewed, relativistic Fe Kα emission line and ionized disk reflection spectrum are revealed in these spectra. Self-consistent models for the Fe Kα emission-line profile and disk reflection spectrum rule out an inner disk radius compatible with a Schwarzschild black hole at more than the 8 σ level of confidence. The best-fit inner disk radius of (2-3)rg suggests that GX 339-4 harbors a black hole with a>=0.8-0.9 (where rg=GM/c2 and a=cJ/GM2, and assuming that reflection in the plunging region is relatively small). This confirms indications for black hole spin based on a Chandra spectrum obtained later in the outburst. The emission line and reflection spectrum also rule out a standard power-law disk emissivity in GX 339-4 a broken power-law form with enhanced emissivity inside ~6rg gives improved fits at more than the 8 σ level of confidence. The extreme red wing of the line and the steep emissivity require a centrally concentrated source of hard X-rays that can strongly illuminate the inner disk. Hard X-ray emission from the base of a jet-enhanced by gravitational light-bending effects-could create the concentrated hard X-ray emission; this process may be related to magnetic connections between the black hole and the inner disk. We discuss these results within the context of recent results from analyses of XTE J1650-500 and MCG -6-30-15, and of models for the inner accretion flow environment around black holes.

Broadband X-Ray Spectra of GX 339-4 and the Geometry of Accreting Black Holes in the Hard State

NASA Technical Reports Server (NTRS)

Tomsick, John A.; Kalemci, Emrah; Kaaret, Philip; Markoff, Sera; Corbel, Stephane; Migliari, Simone; Fender, Rob; Bailyn, Charles D.; Buxton, Michelle M.

2008-01-01

A major question in the study of black hole binaries involves our understanding of the accretion geometry when the sources are in the "hard" state, with an X-ray energy spectrum dominated by a hard power-law component and radio emission coming from a steady "compact" jet. Although the common hard state picture is that the accretion disk is truncated, perhaps at hundreds of gravitational radii (Rg) from the black hole, recent results for the recurrent transient GX 339-4 by Miller and coworkers show evidence for disk material very close to the black hole's innermost stable circular orbit. That work studied GX 339-4 at a luminosity of approximately 5% of the Eddington limit (L(sub Edd) and used parameters from a relativistic reflection model and the presence of a thermal component as diagnostics. Here we use similar diagnostics but extend the study to lower luminosities (2.3% and 0.8% L(sub Edd)) using Swift and RXTE observations of GX 339-4. We detect a thermal component with an inner disk temperature of approximately 0.2 keV at 2.3% L (sub Edd). At both luminosities, we detect broad features due to iron K-alpha that are likely related to reflection of hard X-rays off disk material. If these features are broadened by relativistic effects, they indicate that the material resides within 10 Rg, and the measurements are consistent with the disk's inner radius remaining at approximately 4 Rg down to 0.8% L(sub Edd). However, we also discuss an alternative model for the broadening, and we note that the evolution of the thermal component is not entirely consistent with the constant inner radius interpretation. Finally, we discuss the results in terms of recent theoretical work by Liu and co-workers on the possibility that material may condense out of an Advection-Dominated Accretion Flow to maintain an inner optically thick disk.

41 CFR 301-54.2 - What is disposable pay?

Code of Federal Regulations, 2013 CFR

2013-07-01

... 41 Public Contracts and Property Management 4 2013-07-01 2012-07-01 true What is disposable pay? 301-54.2 Section 301-54.2 Public Contracts and Property Management Federal Travel Regulation System... BILLED TRAVEL CHARGE CARD General Rules § 301-54.2 What is disposable pay? Disposable pay is your...

Evaluation of Student Injuries at the Sergeants Major Course (SMC), Fort Bliss, Texas, August 2013-May 2014

DTIC Science & Technology

2016-06-24

benefit from collection of process metrics, additional factors influencing injury incidence, and additional outcomes of interest. 15. SUBJECT TERMS...therapist at the SMC. Future evaluations would benefit from pre- and post-implementation measurement of additional factors contributing to injury...increased during the SMC. The incorporation of cross-training is likely beneficial, as noted above. Cross-training has also been shown to have benefits

Multiscale finite element modeling of sheet molding compound (SMC) composite structure based on stochastic mesostructure reconstruction

DOE PAGES

Chen, Zhangxing; Huang, Tianyu; Shao, Yimin; ...

2018-03-15

Predicting the mechanical behavior of the chopped carbon fiber Sheet Molding Compound (SMC) due to spatial variations in local material properties is critical for the structural performance analysis but is computationally challenging. Such spatial variations are induced by the material flow in the compression molding process. In this work, a new multiscale SMC modeling framework and the associated computational techniques are developed to provide accurate and efficient predictions of SMC mechanical performance. The proposed multiscale modeling framework contains three modules. First, a stochastic algorithm for 3D chip-packing reconstruction is developed to efficiently generate the SMC mesoscale Representative Volume Element (RVE)more » model for Finite Element Analysis (FEA). A new fiber orientation tensor recovery function is embedded in the reconstruction algorithm to match reconstructions with the target characteristics of fiber orientation distribution. Second, a metamodeling module is established to improve the computational efficiency by creating the surrogates of mesoscale analyses. Third, the macroscale behaviors are predicted by an efficient multiscale model, in which the spatially varying material properties are obtained based on the local fiber orientation tensors. Our approach is further validated through experiments at both meso- and macro-scales, such as tensile tests assisted by Digital Image Correlation (DIC) and mesostructure imaging.« less

Multiscale finite element modeling of sheet molding compound (SMC) composite structure based on stochastic mesostructure reconstruction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Zhangxing; Huang, Tianyu; Shao, Yimin

Predicting the mechanical behavior of the chopped carbon fiber Sheet Molding Compound (SMC) due to spatial variations in local material properties is critical for the structural performance analysis but is computationally challenging. Such spatial variations are induced by the material flow in the compression molding process. In this work, a new multiscale SMC modeling framework and the associated computational techniques are developed to provide accurate and efficient predictions of SMC mechanical performance. The proposed multiscale modeling framework contains three modules. First, a stochastic algorithm for 3D chip-packing reconstruction is developed to efficiently generate the SMC mesoscale Representative Volume Element (RVE)more » model for Finite Element Analysis (FEA). A new fiber orientation tensor recovery function is embedded in the reconstruction algorithm to match reconstructions with the target characteristics of fiber orientation distribution. Second, a metamodeling module is established to improve the computational efficiency by creating the surrogates of mesoscale analyses. Third, the macroscale behaviors are predicted by an efficient multiscale model, in which the spatially varying material properties are obtained based on the local fiber orientation tensors. Our approach is further validated through experiments at both meso- and macro-scales, such as tensile tests assisted by Digital Image Correlation (DIC) and mesostructure imaging.« less

VizieR Online Data Catalog: JHK lightcurves of red giants in the SMC (Takayama+, 2015)

NASA Astrophysics Data System (ADS)

Takayama, M.; Wood, P. R.; Ita, Y.

2017-11-01

This is JHK light curves of 7 oxygen rich stars and 14 carbon stars which show the variability of prominent long secondary periods (LSPs). Those stars are cross-identified with OGLE LSP variables in the Small Magellanic Cloud (Soszynski et al. 2011, J/AcA/61/217). A long-term multiband near-IR photometric survey for variable stars in the Large and Small Magellanic Clouds has been carried out at the South African Astronomical Observatory at Sutherland (Ita et al., in preparation). The SIRIUS camera attached to the IRSF 1.4 m telescope was used for this survey and more than 10 yr of observations in the near-IR bands J(1.25 μm), H(1.63 μm) and KS(2.14 μm) band were obtained. In this work, we select the SMC stars from the SIRIUS data base. We obtained the V- and I-band time series of SMC red giants from the OGLE project (Soszynski et al. 2011, J/AcA/61/217). (2 data files).

NuSTAR AND Swift Observations of the Very High State in GX 339-4: Weighing the Black Hole With X-Rays

NASA Technical Reports Server (NTRS)

Parker, M. L.; Tomsick, J. A.; Kennea, J. A.; Miller, J. M.; Harrison, F. A.; Barret, D.; Boggs, S. E.; Christensen, F. E.; Craig, W. W.; Fabian, A. C.;

46 CFR 54.20-2 - Fabrication for hazardous materials (replaces UW-2(a)).

Code of Federal Regulations, 2010 CFR

2010-10-01

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46 CFR 54.20-2 - Fabrication for hazardous materials (replaces UW-2(a)).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Fabrication for hazardous materials (replaces UW-2(a)). 54.20-2 Section 54.20-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-2 Fabrication for hazardous materials (replaces UW...

46 CFR 54.20-2 - Fabrication for hazardous materials (replaces UW-2(a)).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Fabrication for hazardous materials (replaces UW-2(a)). 54.20-2 Section 54.20-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-2 Fabrication for hazardous materials (replaces UW...

46 CFR 54.20-2 - Fabrication for hazardous materials (replaces UW-2(a)).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Fabrication for hazardous materials (replaces UW-2(a)). 54.20-2 Section 54.20-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-2 Fabrication for hazardous materials (replaces UW...

46 CFR 54.20-2 - Fabrication for hazardous materials (replaces UW-2(a)).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Fabrication for hazardous materials (replaces UW-2(a)). 54.20-2 Section 54.20-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Fabrication by Welding § 54.20-2 Fabrication for hazardous materials (replaces UW...

Detection and period measurements of GX1+4 at hard x ray energies with the SIGMA telescope

NASA Technical Reports Server (NTRS)

Laurent, PH.; Salotti, L.; Lebrun, F.; Paul, J.; Denis, M.; Barret, D.; Jourdain, E.; Roques, J. P.; Churazov, E.; Gilfanov, M.

1992-01-01

The galactic Low Mass X ray Binary GX1+4 was detected by the coded aperture hard X ray gamma ray SIGMA telescope during the Feb. to April 1991 observations of the galactic center regions. The source, whose emission varied during the survey of a factor greater than 40 pct., reached a maximum luminosity in the 40 to 140 energy range of 1.03 x 10(exp 37) erg/s (D = 8.5 kpc), thus approaching the emission level of the 1970 to 1980 high state. Two minute flux pulsations were detected on Mar. 22 and on Mar. 31 and Apr. 1. Comparison with the last period measurements shows that the current spin-down phase of GX1+4 is ending. Concerning the proposed association of this source with the galactic center 511 keV annihilation emission, upper limits were derived.

Torsion-wagging tunneling and vibrational states in hydrazine determined from its ab initio potential energy surface

NASA Astrophysics Data System (ADS)

Łodyga, Wiesław; Makarewicz, Jan

2012-05-01

Geometries, anharmonic vibrations, and torsion-wagging (TW) multiplets of hydrazine and its deuterated species are studied using high-level ab initio methods employing the second-order Møller-Plesset perturbation theory (MP2) as well as the coupled cluster singles and doubles model including connected triple corrections, CCSD(T), in conjunction with extended basis sets containing diffuse and core functions. To describe the splitting patterns caused by tunneling in TW states, the 3D potential energy surface (PES) for the large-amplitude TW modes is constructed. Stationary points in the 3D PES, including equivalent local minima and saddle points are characterized. Using this 3D PES, a flexible Hamiltonian is built numerically and then employed to solve the vibrational problem for TW coupled motion. The calculated ground state rav structure is expected to be more reliable than the experimental one that has been determined using a simplified structural model. The calculated fundamental frequencies allowed resolution of the assignment problems discussed earlier in the literature. The determined energy barriers, including the contributions from the small-amplitude vibrations, to the tunneling of the symmetric and antisymmetric wagging mode of 1997 cm-1 and 3454 cm-1, respectively, are in reasonable agreement with the empirical estimates of 2072 cm-1 and 3312 cm-1, respectively [W. Łodyga et al. J. Mol. Spectrosc. 183, 374 (1997), 10.1006/jmsp.1997.7271]. However, the empirical torsion barrier of 934 cm-1 appears to be overestimated. The ab initio calculations yield two torsion barriers: cis and trans of 744 cm-1 and 2706 cm-1, respectively. The multiplets of the excited torsion states are predicted from the refined 3D PES.

The LF of TP-AGB stars in the LMC/SMC

NASA Technical Reports Server (NTRS)

Bruzual, Gustavo; Charlot, Stephane; GonzalezLopezlira, Rosa; Srinivasan, Sundar; Boyer, Martha L.

2013-01-01

We show that Monte Carlo simulations of the TP-AGB stellar population in the LMC and SMC galaxies using the CB. models produce LF and color distributions that are in closer agreement with observations than those obtained with the BC03 and CB07 models. This is a progress report of work that will be published elsewhere.

Revisiting galactic black hole binary GX 339-4 by using 2007 – 2014 Swift XRT observations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Azizi, Febrie Ahmad; Vierdayanti, Kiki; Putra, Mahasena

2015-09-30

This work aims to study the X-ray properties of the galactic black hole binary GX 339-4. Focus of the study is on exploration of data from Swift-XRT in exclusively photon-counting mode. We use data from 2007 up to August 2014, which contain about 40 pointing observations with level 1 data. The flux of GX 339-4 varies in a factor of 100 during this period of observations. For the purpose of this work, we also try to develop a system to conduct standard SWIFT XRT data reduction automatically, in order to greatly reduce time when working with data bulk, which producesmore » images, lightcurves as well as spectra. We also develop another system to conduct fitting of bulk spectral data with a two-component model, disk blackbody and power-law. The fitting results show that no data have a reduced chi-squared > 2. The fraction of the disk to total flux and the power-law to total flux range from 0.00389 – 0.994 and 0.00605 – 0.996, respectively. From the analysis of the disk component, we obtain the value of the innermost disk radius that does not show any large scale truncation which is in a good agreement with a previous study that used 2007 – 2011 Swift-XRT data, indicating that the systems we developed work properly.« less

SMC X-3: the closest ultraluminous X-ray source powered by a neutron star with non-dipole magnetic field

NASA Astrophysics Data System (ADS)

Tsygankov, S. S.; Doroshenko, V.; Lutovinov, A. A.; Mushtukov, A. A.; Poutanen, J.

2017-09-01

Aims: The magnetic field of accreting neutron stars determines their overall behavior including the maximum possible luminosity. Some models require an above-average magnetic field strength (≳1013 G) in order to explain super-Eddington mass accretion rate in the recently discovered class of pulsating ultraluminous X-ray sources (ULX). The peak luminosity of SMC X-3 during its major outburst in 2016-2017 reached 2.5 × 1039 erg s-1 comparable to that in ULXs thus making this source the nearest ULX-pulsar. Determination of the magnetic field of SMC X-3 is the main goal of this paper. Methods: SMC X-3 belongs to the class of transient X-ray pulsars with Be optical companions, and exhibited a giant outburst in July 2016-March 2017. The source has been observed over the entire outburst with the Swift/XRT and Fermi/GBM telescopes, as well as the NuSTAR observatory. Collected data allowed us to estimate the magnetic field strength of the neutron star in SMC X-3 using several independent methods. Results: Spin evolution of the source during and between the outbursts, and the luminosity of the transition to the so-called propeller regime in the range of (0.3-7) × 1035 erg s-1 imply a relatively weak dipole field of (1-5) × 1012 G. On the other hand, there is also evidence for a much stronger field in the immediate vicinity of the neutron star surface. In particular, transition from super- to sub-critical accretion regime associated with the cease of the accretion column and very high peak luminosity favor a field that is an order of magnitude stronger. This discrepancy makes SMC X-3 a good candidate for possessing significant non-dipolar components of the field, and an intermediate source between classical X-ray pulsars and accreting magnetars which may constitute an appreciable fraction of ULX population.

Age-related changes in albumin elimination in female WAG/Rij rats.

PubMed Central

Horbach, G J; Yap, S H; van Bezooijen, C F

1983-01-01

Albumin elimination rates were determined in 3-, 12-, 24- and 36-month-old female WAG/Rij rats. No change in elimination half-life was found with age. However, as there was an increase in the whole-body albumin pool, a concomitant increase in albumin clearance was observed at between 12 and 36 months of age. It was concluded that the increase in clearance between 12 and 24 months of age was only due to a change in the animal's physiology, whereas between 24 and 36 months of age it was also due to changes in the albumin molecule. The age-related changes in albumin clearance were thought not to be caused by changes in the albumin excretion via the urine or via the gastrointestinal tract. Images Fig. 1. PMID:6661199

Long-Term Properties of Accretion Discs in X-ray Binaries. 1; The Variable Third Period in SMC X-1

NASA Technical Reports Server (NTRS)

Charles, P. A.; Clarkson, W. I.; Coe, M. J.; Laycock, S.; Tout, M.; Wilson, C.; Six, N. Frank (Technical Monitor)

2002-01-01

Long term X-ray monitoring data from the RXTE All Sky Monitor (ASM) reveal that the third (superorbital) period in SMC X-1 is not constant but varies between 40-60 days. A dynamic power spectrum analysis indicates that the third period has been present continuously throughout the five years of ASM observations. This period changed smoothly from 60 days to 45 days and then returned to its former value, on a timescale of approximately 1600 days. During the nearly 4 years of overlap between the CGRO & RXTE missions, the simultaneous BATSE hard X-ray data confirm this variation in SMC X-1. Sources of systematic error and possible artefacts are investigated and found to be incapable of reproducing the results reported here. Our disco cry of such an instability in the superorbital period of SMC X-1 is interpreted in the context of recent theoretical studies of warped, precessing accretion discs. We find that the behaviour of SMC X-1 is consistent with a radiation - driven warping model.

SMC west halo: a slice of the galaxy that is being tidally stripped?. Star clusters trace age and metallicity gradients

NASA Astrophysics Data System (ADS)

Dias, B.; Kerber, L.; Barbuy, B.; Bica, E.; Ortolani, S.

2016-06-01

Context. The evolution and structure of the Magellanic Clouds is currently under debate. The classical scenario in which both the Large and Small Magellanic Clouds (LMC, SMC) are orbiting the Milky Way has been challenged by an alternative in which the LMC and SMC are in their first close passage to our Galaxy. The clouds are close enough to us to allow spatially resolved observation of their stars, and detailed studies of stellar populations in the galaxies are expected to be able to constrain the proposed scenarios. In particular, the west halo (WH) of the SMC was recently characterized with radial trends in age and metallicity that indicate tidal disruption. Aims: We intend to increase the sample of star clusters in the west halo of the SMC with homogeneous age, metallicity, and distance derivations to allow a better determination of age and metallicity gradients in this region. Positions are compared with the orbital plane of the SMC from models. Methods: Comparisons of observed and synthetic V(B-V) colour-magnitude diagrams were used to derive age, metallicity, distance, and reddening for star clusters in the SMC west halo. Observations were carried out using the 4.1 m SOAR telescope. Photometric completeness was determined through artificial star tests, and the members were selected by statistical comparison with a control field. Results: We derived an age of 1.23 ± 0.07 Gyr and [Fe/H] = -0.87 ± 0.07 for the reference cluster NGC 152, compatible with literature parameters. Age and metallicity gradients are confirmed in the WH: 2.6 ± 0.6 Gyr/° and -0.19 ± 0.09 dex/°, respectively. The age-metallicity relation for the WH has a low dispersion in metallicity and is compatible with a burst model of chemical enrichment. All WH clusters seem to follow the same stellar distribution predicted by dynamical models, with the exception of AM-3, which should belong to the counter-bridge. Brück 6 is the youngest cluster in our sample. It is only 130 ± 40 Myr old and

Broadband X-Ray Spectra of GX 339-4 and the Geometry of Accreting Black Holes in the Hard State

NASA Technical Reports Server (NTRS)

Tomsick; Kalemci; Kaaret; Markoff; Corbel; Migliari; Fender; Bailyn; Buxton

2008-01-01

A major question in the study of black hole binaries involves our understanding of the accretion geometry when the sources are in the "hard" state. In this state, the X-ray energy spectrum is dominated by a hard power-law component and radio observations indicate the presence of a steady and powerful "compact" jet. Although the common hard state picture is that the accretion disk is truncated, perhaps at hundreds of gravitational radii (R(sub g)) from the black hole, recent results for the recurrent transient GX 339-4 by Miller and co-workers show evidence for optically thick material very close to the black hole's innermost stable circular orbit. That work focused on an observation of GX 339-4 at a luminosity of about 5% of the Eddington limit (L(sub Edd)) and used parameters from a relativistic reflection model and the presence of a soft, thermal component as diagnostics. In this work, we use similar diagnostics, but extend the study to lower luminosities (2.3% and 0.8% L(sub Edd)) using Swift and RXTE observations of GX 339-4. We detect a thermal component with an inner disk temperature of approx.0.2 keV at 2.3% L(sub Edd). At 0.8% L(sub Edd), the spectrum is consistent with the presence of such a component, but the component is not required with high confidence. At both luminosities, we detect broad features due to iron Ka that are likely related to reflection of hard X-rays off the optically thick material. If these features are broadened by relativistic effects, they indicate that optically thick material resides within 10 R(sub g) down to 0.8% L(sub Edd), and the measurements are consistent with the inner radius of the disk remaining at approx.4 R(sub g) down to this level. However, we also discuss an alternative model for the broadening, and we note that the evolution of the thermal component is not entirely consistent with the constant inner radius interpretation. Finally, we discuss the results in terms of recent theoretical work by Liu and co-workers on

Investigation of injection dose and camera integration time on quantifying pharmacokinetics of a Cy5.5-GX1 probe with dynamic fluorescence imaging in vivo

NASA Astrophysics Data System (ADS)

Dai, Yunpeng; Chen, Xueli; Yin, Jipeng; Kang, Xiaoyu; Wang, Guodong; Zhang, Xianghan; Nie, Yongzhan; Wu, Kaichun; Liang, Jimin

2016-08-01

The aim of this article is to investigate the influence of a tracer injection dose (ID) and camera integration time (IT) on quantifying pharmacokinetics of Cy5.5-GX1 in gastric cancer BGC-823 cell xenografted mice. Based on three factors, including whether or not to inject free GX1, the ID of Cy5.5-GX1, and the camera IT, 32 mice were randomly divided into eight groups and received 60-min dynamic fluorescence imaging. Gurfinkel exponential model (GEXPM) and Lammertsma simplified reference tissue model (SRTM) combined with a singular value decomposition analysis were used to quantitatively analyze the acquired dynamic fluorescent images. The binding potential (Bp) and the sum of the pharmacokinetic rate constants (SKRC) of Cy5.5-GX1 were determined by the SRTM and EXPM, respectively. In the tumor region, the SKRC value exhibited an obvious trend with change in the tracer ID, but the Bp value was not sensitive to it. Both the Bp and SKRC values were independent of the camera IT. In addition, the ratio of the tumor-to-muscle region was correlated with the camera IT but was independent of the tracer ID. Dynamic fluorescence imaging in conjunction with a kinetic analysis may provide more quantitative information than static fluorescence imaging, especially for a priori information on the optimal ID of targeted probes for individual therapy.

MiR-9 Promotes Apoptosis Via Suppressing SMC1A Expression in GBM Cell Lines.

PubMed

Zu, Yong; Zhu, Zhichuan; Lin, Min; Xu, Dafeng; Liang, Yongjun; Wang, Yueqian; Qiao, Zhengdong; Cao, Ting; Yang, Dan; Gao, Lili; Jin, Pengpeng; Zhang, Peng; Fu, Jianjun; Zheng, Jing

2017-01-01

Glioblastomas multiforme (GBM) is the most malignant brain cancer, which presented vast genomic variation with complicated pathologic mechanism. MicroRNA is a delicate post-transcriptional tuner of gene expression in the organisms by targeting and regulating protein coding genes. MiR-9 was reported as a significant biomarker for GBM patient prognosis and a key factor in regulation of GBM cancer stem cells. To explore the effect of miR-9 on GBM cell growth, we over expressed miR-9 in U87 and U251 cells. The cell viability decreased and apoptosis increased after miR-9 overexpression in these cells. To identify the target of miR-9, we scanned miR-9 binding site in the 3'UTRs region of expression SMC1A (structural maintenance of chromosomes 1A) genes and designed a fluorescent reporter assay to measure miR-9 binding to this region. Our results revealed that miR-9 binds to the 3'sUTR region of SMC1A and down-regulated SMC1A expression. Our results indicated that miR-9 was a potential therapeutic target for GBM through triggering apoptosis of cancer cells.

Performance analysis of a new radial-axial flux machine with SMC cores and ferrite magnets

NASA Astrophysics Data System (ADS)

Liu, Chengcheng; Wang, Youhua; Lei, Gang; Guo, Youguang; Zhu, Jianguo

2017-05-01

Soft magnetic composite (SMC) is a popular material in designing of new 3D flux electrical machines nowadays for it has the merits of isotropic magnetic characteristic, low eddy current loss and high design flexibility over the electric steel. The axial flux machine (AFM) with the extended stator tooth tip both in the radial and circumferential direction is a good example, which has been investigated in the last years. Based on the 3D flux AFM and radial flux machine, this paper proposes a new radial-axial flux machine (RAFM) with SMC cores and ferrite magnets, which has very high torque density though the low cost low magnetic energy ferrite magnet is utilized. Moreover, the cost of RAFM is quite low since the manufacturing cost can be reduced by using the SMC cores and the material cost will be decreased due to the adoption of the ferrite magnets. The 3D finite element method (FEM) is used to calculate the magnetic flux density distribution and electromagnetic parameters. For the core loss calculation, the rotational core loss computation method is used based on the experiment results from previous 3D magnetic tester.

SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster.

PubMed

Yan, Rihui; Thomas, Sharon E; Tsai, Jui-He; Yamada, Yukihiro; McKee, Bruce D

2010-02-08

Sister chromatid cohesion is essential to maintain stable connections between homologues and sister chromatids during meiosis and to establish correct centromere orientation patterns on the meiosis I and II spindles. However, the meiotic cohesion apparatus in Drosophila melanogaster remains largely uncharacterized. We describe a novel protein, sisters on the loose (SOLO), which is essential for meiotic cohesion in Drosophila. In solo mutants, sister centromeres separate before prometaphase I, disrupting meiosis I centromere orientation and causing nondisjunction of both homologous and sister chromatids. Centromeric foci of the cohesin protein SMC1 are absent in solo mutants at all meiotic stages. SOLO and SMC1 colocalize to meiotic centromeres from early prophase I until anaphase II in wild-type males, but both proteins disappear prematurely at anaphase I in mutants for mei-S332, which encodes the Drosophila homologue of the cohesin protector protein shugoshin. The solo mutant phenotypes and the localization patterns of SOLO and SMC1 indicate that they function together to maintain sister chromatid cohesion in Drosophila meiosis.

Detailed chemical composition of classical Cepheids in the LMC cluster NGC 1866 and in the field of the SMC

NASA Astrophysics Data System (ADS)

Lemasle, B.; Groenewegen, M. A. T.; Grebel, E. K.; Bono, G.; Fiorentino, G.; François, P.; Inno, L.; Kovtyukh, V. V.; Matsunaga, N.; Pedicelli, S.; Primas, F.; Pritchard, J.; Romaniello, M.; da Silva, R.

2017-12-01

Context. Cepheids are excellent tracers of young stellar populations. They play a crucial role in astrophysics as standard candles. The chemistry of classical Cepheids in the Milky Way is now quite well-known, however despite a much larger sample, the chemical composition of Magellanic Cepheids has been only scarcely investigated. Aims: For the first time, we study the chemical composition of several Cepheids located in the same populous cluster: NGC 1866, in the Large Magellanic Cloud (LMC). To also investigate the chemical composition of Cepheids at lower metallicity, we look at four targets located in the Small Magellanic Cloud (SMC). Our sample allows us to increase the number of Cepheids with known metallicities in the LMC/SMC by 20%/25% and the number of Cepheids with detailed chemical composition in the LMC/SMC by 46%/50%. Methods: We use canonical spectroscopic analysis to determine the chemical composition of Cepheids and provide abundances for a good number of α, iron-peak, and neutron-capture elements. Results: We find that six Cepheids in the LMC cluster NGC 1866 have a very homogeneous chemical composition, also consistent with red giant branch (RGB) stars in the cluster. Period-age relations that include no or average rotation indicate that all the Cepheids in NGC 1866 have a similar age and therefore belong to the same stellar population. Our results are in good agreement with theoretical models accounting for luminosity and radial velocity variations. Using distances based on period-luminosity relations in the near- or mid-infrared, we investigate for the first time the metallicity distribution of the young population in the SMC in the depth direction. Preliminary results show no metallicity gradient along the SMC main body, but our sample is small and does not contain Cepheids in the inner few degrees of the SMC. Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere under ESO programme

TIME-FREQUENCY ANALYSIS OF THE SUPERORBITAL MODULATION OF THE X-RAY BINARY SMC X-1 USING THE HILBERT-HUANG TRANSFORM

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hu, Chin-Ping; Chou, Yi; Yang, Ting-Chang

2011-10-20

The high-mass X-ray binary SMC X-1 exhibits a superorbital modulation with a dramatically varying period ranging between {approx}40 days and {approx}60 days. This research studies the time-frequency properties of the superorbital modulation of SMC X-1 based on the observations made by the All-Sky Monitor (ASM) onboard the Rossi X-ray Timing Explorer (RXTE). We analyzed the entire ASM database collected since 1996. The Hilbert-Huang transform (HHT), developed for non-stationary and nonlinear time-series analysis, was adopted to derive the instantaneous superorbital frequency. The resultant Hilbert spectrum is consistent with the dynamic power spectrum as it shows more detailed information in both themore » time and frequency domains. The RXTE observations show that the superorbital modulation period was mostly between {approx}50 days and {approx}65 days, whereas it changed to {approx}45 days around MJD 50,800 and MJD 54,000. Our analysis further indicates that the instantaneous frequency changed to a timescale of hundreds of days between {approx}MJD 51,500 and {approx}MJD 53,500. Based on the instantaneous phase defined by HHT, we folded the ASM light curve to derive a superorbital profile, from which an asymmetric feature and a low state with barely any X-ray emissions (lasting for {approx}0.3 cycles) were observed. We also calculated the correlation between the mean period and the amplitude of the superorbital modulation. The result is similar to the recently discovered relationship between the superorbital cycle length and the mean X-ray flux for Her X-1.« less

Waste area grouping 2 Phase I task data report: Ecological risk assessment and White Oak Creek watershed screening ecological risk assessment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Efroymson, R.A.; Jackson, B.L.; Jones, D.S.

1996-05-01

This report presents an ecological risk assessment for Waste Area Grouping (WAG) 2 based on the data collected in the Phase I remedial investigation (RI). It serves as an update to the WAG 2 screening ecological risk assessment that was performed using historic data. In addition to identifying potential ecological risks in WAG 2 that may require additional data collection, this report serves to determine whether there are ecological risks of sufficient magnitude to require a removal action or some other expedited remedial process. WAG 2 consists of White Oak Creek (WOC) and its tributaries downstream of the Oak Ridgemore » National Laboratory (ORNL) main plant area, White Oak Lake (WOL), the White Oak Creek Embayment of the Clinch River, associated flood plains, and the associated groundwater. The WOC system drains the WOC watershed, an area of approximately 16.8 km{sup 2} that includes ORNL and associated WAGs. The WOC system has been exposed to contaminants released from ORNL and associated operations since 1943 and continues to receive contaminants from adjacent WAGs.« less

Observations of 12-200 keV X-rays from GX 339-4

NASA Technical Reports Server (NTRS)

Nolan, P. L.; Gruber, D. E.; Knight, F. K.; Matteson, J. L.; Peterson, L. E.; Levine, A. M.; Lewin, W. H. G.; Primini, F. A.

1982-01-01

X-ray spectra of GX 339-4 measured on three occasions in 1977 and 1978 are presented. These are the first reported measurements above 10 keV. The spectra can be described as the superposition of a soft component, which is dominant below about 20 keV, and a hard component at higher energy. Simultaneous measurements at lower energy show that the soft component vanished during the observation in early 1978. The behavior of these two components is similar to that of the spectrum of Cygnus X-1; this reinforces the previously noted resemblance in rapid X-ray variability.

A curious case of the accretion-powered X-ray pulsar GX 1+4

NASA Astrophysics Data System (ADS)

Jaisawal, Gaurava K.; Naik, Sachindra; Gupta, Shivangi; Chenevez, Jérôme; Epili, Prahlad

2018-04-01

We present detailed spectral and timing studies using a NuSTAR observation of GX 1+4 in October 2015 during an intermediate intensity state. The measured spin period of 176.778 s is found to be one of the highest values since its discovery. In contrast to a broad sinusoidal-like pulse profile, a peculiar sharp peak is observed in profiles below ˜25 keV. The profiles at higher energies are found to be significantly phase-shifted compared to the soft X-ray profiles. Broadband energy spectra of GX 1+4, obtained from NuSTAR and Swift observations, are described with various continuum models. Among these, a two component model consisting of a bremsstrahlung and a blackbody component is found to best-fit the phase-averaged and phase-resolved spectra. Physical models are also used to investigate the emission mechanism in the pulsar, which allows us to estimate the magnetic field strength to be in ˜(5-10)× 1012 G range. Phase-resolved spectroscopy of NuSTAR observation shows a strong blackbody emission component in a narrow pulse phase range. This component is interpreted as the origin of the peculiar peak in the pulse profiles below ≤25 keV. The size of emitting region is calculated to be ˜400 m. The bremsstrahlung component is found to dominate in hard X-rays and explains the nature of simple profiles at high energies.

A curious case of the accretion-powered X-ray pulsar GX 1+4

NASA Astrophysics Data System (ADS)

Jaisawal, Gaurava K.; Naik, Sachindra; Gupta, Shivangi; Chenevez, Jérôme; Epili, Prahlad

2018-07-01

We present detailed spectral and timing studies using a NuSTAR observation of GX 1+4 in 2015 October during an intermediate-intensity state. The measured spin period of 176.778 s is found to be one of the highest values since its discovery. In contrast to a broad sinusoidal-like pulse profile, a peculiar sharp peak is observed in profiles below ˜25 keV. The profiles at higher energies are found to be significantly phase shifted compared to the soft X-ray profiles. Broad-band energy spectra of GX 1+4, obtained from NuSTAR and Swift observations, are described with various continuum models. Among these, a two-component model consisting of a bremsstrahlung and a blackbody component is found to best fit the phase-averaged and phase-resolved spectra. Physical models are also used to investigate the emission mechanism in the pulsar, which allows us to estimate the magnetic field strength to be in ˜(5-10) × 1012 G range. Phase-resolved spectroscopy of NuSTAR observation shows a strong blackbody emission component in a narrow pulse phase range. This component is interpreted as the origin of the peculiar peak in the pulse profiles below ≤25 keV. The size of emitting region is calculated to be ˜400 m. The bremsstrahlung component is found to dominate in hard X-rays and explains the nature of simple profiles at high energies.

Galaptin Mediates the Effect of Hypergravity on Vascular Smooth Muscle cell (SMC) Adhesion to Laminin Containing Matrices

NASA Technical Reports Server (NTRS)

Enahora, Fatisha T.; Bosah, Francis N.; Harris-Hooker, Sandra; Sanford, Gary L.

1997-01-01

Galaptin, an endogenous beta-galactoside specific lectin, has been reported to bind to laminin and subsequently decrease the binding of SMC. Cellular function depend on cell:matrix interactions. Hypergravity (HGrav) affect a number of cellular functions, yet little is known about its affect on cell adhesion. We examined the possibility that galaptin mediates the effects of hypergravity on SMC adherence. Confluent primate aorta SMC cultures were subjected to Hgrav (centrifuged at 6G) for 24 and 48 hr. Cells were non-enzymatically dispersed, pretreated with antisense (AS-oligo) or control sense (SS-oligo) oligonucleotides to galaptin mRNA (0.01 micro g/ml), then seeded in uncoated or ECL-matrix coated plates. Adhesion of cells were monitored after 6 hr. HGrav increased adhesion by 100-300% compared to controls. AS-oligo decreased adhesion for both HGrav and control cells. SS-oligo did not affect adhesion for either HGrav or control cells. These studies show that HGrav affects cell adhesion and that galaptin expression is required for this effect.

CO 2 water-alternating-gas injection for enhanced oil recovery: Optimal well controls and half-cycle lengths

DOE PAGES

Chen, Bailian; Reynolds, Albert C.

2018-03-11

We report that CO 2 water-alternating-gas (WAG) injection is an enhanced oil recovery method designed to improve sweep efficiency during CO 2 injection with the injected water to control the mobility of CO 2 and to stabilize the gas front. Optimization of CO 2 -WAG injection is widely regarded as a viable technique for controlling the CO 2 and oil miscible process. Poor recovery from CO 2 -WAG injection can be caused by inappropriately designed WAG parameters. In previous study (Chen and Reynolds, 2016), we proposed an algorithm to optimize the well controls which maximize the life-cycle net-present-value (NPV). However,more » the effect of injection half-cycle lengths for each injector on oil recovery or NPV has not been well investigated. In this paper, an optimization framework based on augmented Lagrangian method and the newly developed stochastic-simplex-approximate-gradient (StoSAG) algorithm is proposed to explore the possibility of simultaneous optimization of the WAG half-cycle lengths together with the well controls. Finally, the proposed framework is demonstrated with three reservoir examples.« less

CO 2 water-alternating-gas injection for enhanced oil recovery: Optimal well controls and half-cycle lengths

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Bailian; Reynolds, Albert C.

We report that CO 2 water-alternating-gas (WAG) injection is an enhanced oil recovery method designed to improve sweep efficiency during CO 2 injection with the injected water to control the mobility of CO 2 and to stabilize the gas front. Optimization of CO 2 -WAG injection is widely regarded as a viable technique for controlling the CO 2 and oil miscible process. Poor recovery from CO 2 -WAG injection can be caused by inappropriately designed WAG parameters. In previous study (Chen and Reynolds, 2016), we proposed an algorithm to optimize the well controls which maximize the life-cycle net-present-value (NPV). However,more » the effect of injection half-cycle lengths for each injector on oil recovery or NPV has not been well investigated. In this paper, an optimization framework based on augmented Lagrangian method and the newly developed stochastic-simplex-approximate-gradient (StoSAG) algorithm is proposed to explore the possibility of simultaneous optimization of the WAG half-cycle lengths together with the well controls. Finally, the proposed framework is demonstrated with three reservoir examples.« less

Dietary 2-oxoglutarate mitigates gastrectomy-evoked structural changes in cartilage of female rats.

PubMed

Dobrowolski, Piotr; Tomaszewska, Ewa; Kurlak, Paulina; Pierzynowski, Stefan G

2016-01-01

Gastrectomy (Gx) leads to osteopenia/osteoporosis in humans and animals. However, little is known about the influence of Gx on the cartilage in this regard. Recent studies have demonstrated a protective effect of 2-oxoglutaric acid (2-Ox) on bone and cartilage. Hence, the purpose of this study was to investigate whether 2-Ox can mitigate eventual Gx-induced cartilage impairment. Twenty female Sprague-Dawley rats were subjected to Gx and randomly divided into two groups: Gx + 2-Ox and Gx. Another 20 rats were sham-operated (ShO) and randomly divided into two groups: ShO + 2-Ox and ShO. The daily dose of 2-Ox administered to the rats in the drinking water was 0.43 g per 100 g rat. After eight weeks, rats were euthanized and femora and tibiae were collected. Histology and histomorphometry analyses of the articular cartilage and the growth plate were done. Gx resulted in a 32% (±44.5 femur, ±35.8 tibia) decrease in overall thickness of articular cartilage in both bones (femur: ShO 279.1 ± 48.5 vs. Gx 190.2 ± 38.4 µm, tibia: ShO 222.9 ± 50.3 µm vs. Gx 151.3 ± 52.6 µm) (in some zones up to 58 ± 28.0%), and in the growth plate up to 20% (±22.4) (femur: ShO 243.0 ± 34.0 vs. Gx 207.0 ± 33.7 µm, tibia: ShO 220.0 ± 24.6 µm vs. Gx 171.1 ± 16.1 µm). Gx altered the spatial distribution of thick and thin collagen fibers, and chondrocyte shape and size. 2-Ox administration prevented the reduction in both cartilages thickness (Gx + 2-Ox: articular cartilage 265.2 ± 53.8 µm, 235.6 ± 42.7 µm, growth plate 236.7 ± 39.2 µm, 191.3 ± 16.5 µm in femur and tibia, respectively), and abolished the spatial changes in collagen distribution and structure induced by Gx. Gx affects cartilage structure and thickness, however, 2-Ox administration mitigates these effects and showed protective and stimulatory properties. Our observations suggest that dietary 2-Ox can be used to offset

Dietary 2-oxoglutarate mitigates gastrectomy-evoked structural changes in cartilage of female rats

PubMed Central

Tomaszewska, Ewa; Kurlak, Paulina; Pierzynowski, Stefan G

2015-01-01

Gastrectomy (Gx) leads to osteopenia/osteoporosis in humans and animals. However, little is known about the influence of Gx on the cartilage in this regard. Recent studies have demonstrated a protective effect of 2-oxoglutaric acid (2-Ox) on bone and cartilage. Hence, the purpose of this study was to investigate whether 2-Ox can mitigate eventual Gx-induced cartilage impairment. Twenty female Sprague-Dawley rats were subjected to Gx and randomly divided into two groups: Gx + 2-Ox and Gx. Another 20 rats were sham-operated (ShO) and randomly divided into two groups: ShO + 2-Ox and ShO. The daily dose of 2-Ox administered to the rats in the drinking water was 0.43 g per 100 g rat. After eight weeks, rats were euthanized and femora and tibiae were collected. Histology and histomorphometry analyses of the articular cartilage and the growth plate were done. Gx resulted in a 32% (±44.5 femur, ±35.8 tibia) decrease in overall thickness of articular cartilage in both bones (femur: ShO 279.1 ± 48.5 vs. Gx 190.2 ± 38.4 µm, tibia: ShO 222.9 ± 50.3 µm vs. Gx 151.3 ± 52.6 µm) (in some zones up to 58 ± 28.0%), and in the growth plate up to 20% (±22.4) (femur: ShO 243.0 ± 34.0 vs. Gx 207.0 ± 33.7 µm, tibia: ShO 220.0 ± 24.6 µm vs. Gx 171.1 ± 16.1 µm). Gx altered the spatial distribution of thick and thin collagen fibers, and chondrocyte shape and size. 2-Ox administration prevented the reduction in both cartilages thickness (Gx + 2-Ox: articular cartilage 265.2 ± 53.8 µm, 235.6 ± 42.7 µm, growth plate 236.7 ± 39.2 µm, 191.3 ± 16.5 µm in femur and tibia, respectively), and abolished the spatial changes in collagen distribution and structure induced by Gx. Gx affects cartilage structure and thickness, however, 2-Ox administration mitigates these effects and showed protective and stimulatory properties. Our observations suggest that dietary 2-Ox can be used to offset

Heterozygous truncation mutations of the SMC1A gene cause a severe early onset epilepsy with cluster seizures in females: Detailed phenotyping of 10 new cases.

PubMed

Symonds, Joseph D; Joss, Shelagh; Metcalfe, Kay A; Somarathi, Suresh; Cruden, Jamie; Devlin, Anita M; Donaldson, Alan; DiDonato, Nataliya; Fitzpatrick, David; Kaiser, Frank J; Lampe, Anne K; Lees, Melissa M; McLellan, Ailsa; Montgomery, Tara; Mundada, Vivek; Nairn, Lesley; Sarkar, Ajoy; Schallner, Jens; Pozojevic, Jelena; Parenti, Ilaria; Tan, Jeen; Turnpenny, Peter; Whitehouse, William P; Zuberi, Sameer M

2017-04-01

The phenotype of seizure clustering with febrile illnesses in infancy/early childhood is well recognized. To date the only genetic epilepsy consistently associated with this phenotype is PCDH19, an X-linked disorder restricted to females, and males with mosaicism. The SMC1A gene, which encodes a structural component of the cohesin complex is also located on the X chromosome. Missense variants and small in-frame deletions of SMC1A cause approximately 5% of Cornelia de Lange Syndrome (CdLS). Recently, protein truncating mutations in SMC1A have been reported in five females, all of whom have been affected by a drug-resistant epilepsy, and severe developmental impairment. Our objective was to further delineate the phenotype of SMC1A truncation. Female cases with de novo truncation mutations in SMC1A were identified from the Deciphering Developmental Disorders (DDD) study (n = 8), from postmortem testing of an affected twin (n = 1), and from clinical testing with an epilepsy gene panel (n = 1). Detailed information on the phenotype in each case was obtained. Ten cases with heterozygous de novo mutations in the SMC1A gene are presented. All 10 mutations identified are predicted to result in premature truncation of the SMC1A protein. All cases are female, and none had a clinical diagnosis of CdLS. They presented with onset of epileptic seizures between <4 weeks and 28 months of age. In the majority of cases, a marked preponderance for seizures to occur in clusters was noted. Seizure clusters were associated with developmental regression. Moderate or severe developmental impairment was apparent in all cases. Truncation mutations in SMC1A cause a severe epilepsy phenotype with cluster seizures in females. These mutations are likely to be nonviable in males. Wiley Periodicals, Inc. © 2017 International League Against Epilepsy.

Structural mapping of the coiled-coil domain of a bacterial condensin and comparative analyses across all domains of life suggest conserved features of SMC proteins.

PubMed

Waldman, Vincent M; Stanage, Tyler H; Mims, Alexandra; Norden, Ian S; Oakley, Martha G

2015-06-01

The structural maintenance of chromosomes (SMC) proteins form the cores of multisubunit complexes that are required for the segregation and global organization of chromosomes in all domains of life. These proteins share a common domain structure in which N- and C- terminal regions pack against one another to form a globular ATPase domain. This "head" domain is connected to a central, globular, "hinge" or dimerization domain by a long, antiparallel coiled coil. To date, most efforts for structural characterization of SMC proteins have focused on the globular domains. Recently, however, we developed a method to map interstrand interactions in the 50-nm coiled-coil domain of MukB, the divergent SMC protein found in γ-proteobacteria. Here, we apply that technique to map the structure of the Bacillus subtilis SMC (BsSMC) coiled-coil domain. We find that, in contrast to the relatively complicated coiled-coil domain of MukB, the BsSMC domain is nearly continuous, with only two detectable coiled-coil interruptions. Near the middle of the domain is a break in coiled-coil structure in which there are three more residues on the C-terminal strand than on the N-terminal strand. Close to the head domain, there is a second break with a significantly longer insertion on the same strand. These results provide an experience base that allows an informed interpretation of the output of coiled-coil prediction algorithms for this family of proteins. A comparison of such predictions suggests that these coiled-coil deviations are highly conserved across SMC types in a wide variety of organisms, including humans. © 2015 Wiley Periodicals, Inc.

Degeneracy lifting due to thermal fluctuations around the frustration point between anticlinic antiferroelectric SmC(A)* and synclinic ferroelectric SmC*.

PubMed

Sandhya, K L; Chandani, A D L; Fukuda, Atsuo; Vij, Jagdish K; Emelyanenko, A V; Ishikawa, Ken

2013-01-01

In the binary mixture phase diagram of MC881 and MC452, the borderline between anticlinic antiferroelectric SmC(A)(*) and synclinic ferroelectric SmC(*) becomes apparently parallel to the temperature ordinate axis at the critical concentration r(c). The free energy difference between SmC(A)(*) and SmC^{*} is extremely small in a wide temperature range near r(c). In such circumstances, by observing Bragg reflection spectra due to the director helical structure and electric-field-induced birefringence, we have observed the continuous change from SmC(A)(*) to SmC(*) for rSmC(*) temperature region for r>/~r(c). These intriguing phenomena have been explained, successfully at least in the high-temperature region, by a thermal equilibrium between the synclinic and anticlinic orderings and the resulting Boltzmann distribution for the ratio between them; the thermal equilibrium is considered to be attained in a nonuniform defect-assisted way through solitary waves moving around dynamically. We have also discussed qualitatively an important role played by the effective long-range interlayer interactions in the low-temperature region.

The effect of surface alignment on analog control of director rotation in polarization stiffened SmC* devices

NASA Astrophysics Data System (ADS)

Reznikov, Mitya; Lopatina, Lena M.; O'Callaghan, Michael J.; Bos, Philip J.

2011-03-01

The effect of surface alignment on the achievement of analog ("V"-shaped) electric field control of director rotation in SmC* liquid crystal devices is investigated experimentally and through numerical modeling. Ferroelectric SmC* liquid crystals are intrinsically analog and thresholdless, i.e. the director can be rotated freely around the tilt cone. Whether or not a SmC* liquid crystal cell exhibits thresholdless switching depends strongly on the influence of the cell's alignment layers, on the magnitude of the liquid crystal's spontaneous polarization, and on whether smectic layers adopt a bookshelf or chevron configuration. To study the effect of the surface alignment layers, we have exploited a technique for the vertical (bookshelf) alignment of the smectic layers that does not depend on surface anisotropy. The alignment technique allows an experimental study of the influence of surfaces spanning a wide range of pretilt angles, azimuthal and zenithal anchoring energies. This technique is used to study the effect of surfaces on the threshold behavior of director rotation in SmC* materials under the influence of an electric field. The alignment technique also allowed us to use a high-PS liquid crystal material having an I-A-C phase sequence and reduced layer shrinkage thought to be well suited to thresholdless switching. We show that the alignment layer has a strong effect, and that excellent analog response can be achieved for the case of alignment layers which promote homeotropic director orientation. We further model and discuss the potential effect of a thin layer of nematic at the surface and the possibility of gliding of the easy axis during switching.

Field sampling and analysis plan for the remedial investigation of Waste Area Grouping 2 at Oak Ridge National Laboratory, Oak Ridge, Tennessee. Environmental Restoration Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boston, H.L.; Ashwood, T.L.; Borders, D.M.

1992-02-01

This field sampling and analysis (S & A) plan has been developed as part of the Department of Energy`s (DOE`s) remedial investigation (RI) of Waste Area Grouping (WAG) 2 at Oak Ridge National Laboratory (ORNL) located in Oak Ridge, Tennessee. The S & A plan has been written in support of the remedial investigation (RI) plan for WAG 2 (ORNL 1990). WAG 2 consists of White Oak Creek (WOC) and its tributaries downstream of the ORNL main plant area, White Oak Lake (WOL), White Oak Creek embayment (WOCE) on the Clinch River, and the associated floodplain and subsurface environment (Fig.more » 1.1). The WOC system is the surface drainage for the major ORNL WAGs and has been exposed to a diversity of contaminants from operations and waste disposal activities in the WOC watershed. WAG 2 acts as a conduit through which hydrologic fluxes carry contaminants from upgradient areas to the Clinch River. Water, sediment, soil, and biota in WAG 2 are contaminated and continue to receive contaminants from upgradient WAGs. This document describes the following: an overview of the RI plan, background information for the WAG 2 system, and objectives of the S & A plan; the scope and implementation of the first 2 years of effort of the S & A plan and includes recent information about contaminants of concern, organization of S & A activities, interactions with other programs, and quality assurance specific to the S & A activities; provides details of the field sampling plans for sediment, surface water, groundwater, and biota, respectively; and describes the sample tracking and records management plan.« less

Advances for the Topographic Characterisation of SMC Materials

PubMed Central

Calvimontes, Alfredo; Grundke, Karina; Müller, Anett; Stamm, Manfred

2009-01-01

For a comprehensive study of Sheet Moulding Compound (SMC) surfaces, topographical data obtained by a contact-free optical method (chromatic aberration confocal imaging) were systematically acquired to characterise these surfaces with regard to their statistical, functional and volumetrical properties. Optimal sampling conditions (cut-off length and resolution) were obtained by a topographical-statistical procedure proposed in the present work. By using different length scales specific morphologies due to the influence of moulding conditions, metallic mould topography, glass fibre content and glass fibre orientation can be characterized. The aim of this study is to suggest a systematic topographical characterization procedure for composite materials in order to study and recognize the influence of production conditions on their surface quality.

9 CFR 54.2 - Cooperative agreements and memoranda of understanding with States.

Code of Federal Regulations, 2010 CFR

2010-01-01

... of understanding with States. 54.2 Section 54.2 Animals and Animal Products ANIMAL AND PLANT HEALTH... DISEASES CONTROL OF SCRAPIE § 54.2 Cooperative agreements and memoranda of understanding with States. APHIS will execute cooperative agreements and/or memoranda of understanding with the animal health agency of...

An Ultra-fast X-Ray Disk Wind in the Neutron Star Binary GX 340+0

NASA Astrophysics Data System (ADS)

Miller, J. M.; Raymond, J.; Cackett, E.; Grinberg, V.; Nowak, M.

2016-05-01

We present a spectral analysis of a brief Chandra/HETG observation of the neutron star low-mass X-ray binary GX 340+0. The high-resolution spectrum reveals evidence of ionized absorption in the Fe K band. The strongest feature, an absorption line at approximately 6.9 keV, is required at the 5σ level of confidence via an F-test. Photoionization modeling with XSTAR grids suggests that the line is the most prominent part of a disk wind with an apparent outflow speed of v = 0.04c. This interpretation is preferred at the 4σ level over a scenario in which the line is H-like Fe xxvi at a modest redshift. The wind may achieve this speed owing to its relatively low ionization, enabling driving by radiation pressure on lines; in this sense, the wind in GX 340+0 may be the stellar-mass equivalent of the flows in broad absorption line quasars. If the gas has a unity volume filling factor, the mass ouflow rate in the wind is over 10-5 M ⊙ yr-1, and the kinetic power is nearly 1039 erg s-1 (or, 5-6 times the radiative Eddington limit for a neutron star). However, geometrical considerations—including a small volume filling factor and low covering factor—likely greatly reduce these values.

Searches for millisecond pulsations in low-mass X-ray binaries, 2

NASA Technical Reports Server (NTRS)

Vaughan, B. A.; Van Der Klis, M.; Wood, K. S.; Norris, J. P.; Hertz, P.; Michelson, P. F.; Paradijs, J. Van; Lewin, W. H. G.; Mitsuda, K.; Penninx, W.

1994-01-01

Coherent millisecond X-ray pulsations are expected from low-mass X-ray binaries (LMXBs), but remain undetected. Using the single-parameter Quadratic Coherence Recovery Technique (QCRT) to correct for unknown binary orbit motion, we have performed Fourier transform searches for coherent oscillations in all long, continuous segments of data obtained at 1 ms time resolution during Ginga observations of LMXB. We have searched the six known Z sources (GX 5-1, Cyg X-2, Sco X-1, GX 17+2, GX 340+0, and GX 349+2), seven of the 14 known atoll sources (GX 3+1. GX 9+1, GX 9+9, 1728-33. 1820-30, 1636-53 and 1608-52), the 'peculiar' source Cir X-1, and the high-mass binary Cyg X-3. We find no evidence for coherent pulsations in any of these sources, with 99% confidence limits on the pulsed fraction between 0.3% and 5.0% at frequencies below the Nyquist frequency of 512 Hz. A key assumption made in determining upper limits in previous searches is shown to be incorrect. We provide a recipe for correctly setting upper limits and detection thresholds. Finally we discuss and apply two strategies to improve sensitivity by utilizing multiple, independent, continuous segments of data with comparable count rates.

Multiwavelength Observations of the 2002 Outburst of GX 339-4: Two Patterns of X-Ray-Optical/Near-Infrared Behavior

NASA Astrophysics Data System (ADS)

Homan, Jeroen; Buxton, Michelle; Markoff, Sera; Bailyn, Charles D.; Nespoli, Elisa; Belloni, Tomaso

2005-05-01

We report on quasi-simultaneous Rossi X-Ray Timing Explorer and optical/near-infrared (NIR) observations of the black hole candidate X-ray transient GX 339-4. Our observations were made over a time span of more than 8 months in 2002 and cover the initial rise and transition from a hard to a soft spectral state in X-rays. Two distinct patterns of correlated X-ray-optical/NIR behavior were found. During the hard state, the optical/NIR and X-ray fluxes correlated well, with a NIR versus X-ray flux power-law slope similar to that of the correlation found between X-ray and radio fluxes in previous studies of GX 339-4 and other black hole binaries. As the source went through an intermediate state, the optical/NIR fluxes decreased rapidly, and once it had entered the spectrally soft state, the optical/NIR spectrum of GX 339-4 was much bluer, and the ratio of X-ray to NIR flux was higher by a factor of more than 10 compared to the hard state. In the spectrally soft state, changes in the NIR preceded those in the soft X-rays by more than 2 weeks, indicating a disk origin of the NIR emission and providing a measure of the viscous timescale. A sudden onset of NIR flaring of ~0.5 mag on a timescale of 1 day was also observed during this period. We present spectral energy distributions, including radio data, and discuss possible sources for the optical/NIR emission. We conclude that, in the hard state, this emission probably originates in the optically thin part of a jet and that in none of the X-ray states is X-ray reprocessing the dominant source of optical/NIR emission. Finally, comparing the light curves from the all-sky monitor (ASM) and Proportional Counter Array (PCA) instruments, we find that the X-ray/NIR delay depends critically on the sensitivity of the X-ray detector, with the delay inferred from the PCA (if present at all) being a factor of 3-6 times shorter than the delay inferred from the ASM; this may be important in interpreting previously reported X

Cannabis agonist injection effect on the coupling architecture in cortex of WAG/Rij rats during absence seizures

NASA Astrophysics Data System (ADS)

Sysoeva, Marina V.; Kuznetsova, Galina D.; van Rijn, Clementina M.; Sysoev, Ilya V.

2016-04-01

WAG/Rij rats are well known genetic model of absence epilepsy, which is traditionally considered as a nonconvulsive generalised epilepsy of unknown aetiology. In current study the effect of (R)-(+)-WIN 55,212-2 (cannabis agonist) injection on the coupling between different parts of cortex was studied on 27 male 8 month old rats using local field potentials. Recently developed non-linear adapted Granger causality approach was used as a primary method. It was shown that first 2 hours after the injection the coupling between most channel pairs rises in comparison with the spontaneous activity, whilst long after the injection (2-6 hours) it drops down. The coupling increase corresponds to the mentioned before treatment effect, when the number and the longitude of seizures significantly decreases. However the subsequent decrease of the coupling in the cortex is accompanied by the dramatic increase of the longitude and the number of seizures. This assumes the hypothesis that a relatively higher coupling in the cortical network can prevent the seizure propagation and generalisation.

Ultraviolet studies of O and B stars in the LMC cluster NGC 2100, the SMC cluster NGC 330 and the Galactic cluster NGC 6530

NASA Technical Reports Server (NTRS)

Boehm-Vitense, E.; Hodge, P.

1984-01-01

High-resolution and low-resolution IUE spectra of O and B stars in the LMC cluster NGC 2100, the SMC cluster NGC 330, and the young Galactic cluster NGC 6530 are investigated. Temperatures and luminosities are determined. In the LMC and SMC clusters, the most luminous stars are evolved stars on the horizontal supergiant branch, while in NGC 6530 the stars are all still on the main sequence. Extinction laws were determined. They confirm the known differences between LMC and Galactic extinctions. No mass loss was detected for the evolved B stars in the LMC and SMC clusters, while the high-luminosity stars in NGC 6530 show P Cygni profiles.

A Comparison of the Variability of the Symbiotic X-ray Binaries GX 1+4, 4U 1954+31, and 4U 1700+24 from Swift/BAT and RXTE/ASM Observations

NASA Technical Reports Server (NTRS)

Corbet, R. H. D.; Sokoloski, J. L.; Mukai, K.; Markwardt, C. B.; Tueller, J.

2007-01-01

We present an analysis of the X-ray variability of three symbiotic X-ray binaries, GX 1+4, 4U 1700+24, and 4U 1954+31, using observations made with the Swift Burst Alert Telescope (BAT) and the Rossi X-ray Timing Explorer (RXTE) All-Sky Monitor (ASM). Observations of 4U 1954+31 with the Swift BAT show modulation at a period near 5 hours. Models to explain this modulation are discussed including the presence of an exceptionally slow X-ray pulsar in the system and accretion instabilities. We conclude that the most likely interpretation is that 4U 1954+31 contains one of the slowest known X-ray pulsars. Unlike 4U 1954+31, neither GX 1+4 nor 4U 1700+24 show any evidence for modulation on a timescale of hours. An analysis of the RXTE ASM light curves of GX l+4, 4U 1700+24, and 4U 1954+31 does not show the presence of periodic modulation in any source, although there is considerable variability on long timescales for all three sources. There is no modulation in GX 1+4 on either the optical 1161 day orbital period or a previously reported 304 day X-ray period. For 4U 1700+24 we do not confirm the 404 day period previously proposed for this source from a shorter duration ASM light curve.

Vitamin K2 improves femoral bone strength without altering bone mineral density in gastrectomized rats.

PubMed

Iwamoto, Jun; Sato, Yoshihiro; Matsumoto, Hideo

2014-01-01

Gastrectomy (GX) induces osteopenia in rats. The present study examined the skeletal effects of vitamin K2 in GX rats. Thirty male Sprague-Dawley rats (12 wk old) were randomized by the stratified weight method into the following three groups of 10 animals each: sham operation (control) group; GX group; and GX+oral vitamin K2 (menatetrenone, 30 mg/kg, 5 d/wk) group. Treatment was initiated at 1 wk after surgery. After 6 wk of treatment, the bone mineral content (BMC), bone mineral density (BMD), and mechanical strength of the femoral diaphysis and distal metaphysis were determined by peripheral quantitative computed tomography and mechanical strength tests, respectively. GX induced decreases in the BMC, BMD, and ultimate force of the femoral diaphysis and distal metaphysis. Vitamin K2 did not significantly influence the BMC or BMD of the femoral diaphysis or distal metaphysis in GX rats, but attenuated the decrease in the ultimate force and increased the stiffness of the femoral diaphysis. The present study showed that administration of vitamin K2 to GX rats improved the bone strength of the femoral diaphysis without altering the BMC or BMD, suggesting effects of vitamin K2 on the cortical bone quality.

A de novo atypical ring sSMC(22) characterized by array CGH in a boy with cat-eye syndrome.

PubMed

Haltrich, Irén; Pikó, Henriett; Kiss, Eszter; Tóth, Zsuzsa; Karcagi, Veronika; Fekete, György

2014-01-01

Microduplications 22q11 have been characterized as a genomic duplication syndrome mediated by nonallelic homologous recombination between region-specific low-copy repeats. Here we report on a 19 years old boy with intellectual disability having an unexpected structurally complex ring small supernumerary marker chromosome (sSMC) originated from a larger trisomy and a smaller tetrasomy of proximal 22q11 harboring additional copies of cat eye syndrome critical regions genes. PRINCIPAL CLINICAL FEATURES WERE: anorectal and urogenital malformations, total anomalous pulmonary venous return with secundum ASD, hearing defect, preauricular pits, seizure and eczema. The proband also presented some rare or so far not reported clinical findings such as hyperinsulinaemia, severe immunodeficiency and grave cognitive deficits. Chromosome analysis revealed a mosaic karyotype with the presence of a small ring-like marker in 60% of cells. Array CGH detected approximately an 1,2 Mb single and a 0,2 Mb double copy gain of the proximal long arm of chromosome 22. The 1,3 Mb intervening region of chromosome 22 from centromere to the breakpoints showed no copy alteration. The karyotype of the patient was defined as 47,XY,+mar[60]/46,XY[40].ish idic r(22)(q11.1.q11.21) × 4.arr 22q11(17,435, 645-18,656,678) × 3,(17,598,642-17,799,783) × 4 dn. The present report is the first one with a detailed description of clinical presentation in a patient carrying an atypical size ring sSMC (22) analyzed by array CGH. The specialty of the finding is emphasized by the fact that although the patient had a mosaic sSMC and the amplified region was smaller than in typical cat eye syndrome cases, the clinical presentation was severe.

Youth appraisals of inter-parental conflict and genetic and environmental contributions to attention-deficit hyperactivity disorder: examination of GxE effects in a twin sample.

PubMed

Nikolas, Molly; Klump, Kelly L; Burt, S Alexandra

2012-05-01

Identification of gene x environment interactions (GxE) for attention-deficit hyperactivity disorder (ADHD) is a crucial component to understanding the mechanisms underpinning the disorder, as prior work indicates large genetic influences and numerous environmental risk factors. Building on prior research, children's appraisals of self-blame were examined as a psychosocial moderator of latent etiological influences on ADHD via biometric twin models, which provide an omnibus test of GxE while managing the potential confound of gene-environment correlation. Participants were 246 twin pairs (total n = 492) ages 6-16 years. ADHD behaviors were assessed via mother report on the Child Behavior Checklist. To assess level of self-blame, each twin completed the Children's Perception of Inter-parental Conflict scale. Two biometric GxE models were fit to the data. The first model revealed a significant decrease in genetic effects and a significant increase in unique environmental influences on ADHD with increasing levels of self-blame. These results generally persisted even after controlling for confounding effects due to gene-environment correlation in the second model. Results suggest that appraisals of self-blame in relation to inter-parental conflict may act as a key moderator of etiological contributions to ADHD.

Remote monitoring of Xpert® MTB/RIF testing in Mozambique: results of programmatic implementation of GxAlert.

PubMed

Cowan, J; Michel, C; Manhiça, I; Mutaquiha, C; Monivo, C; Saize, D; Beste, J; Creswell, J; Codlin, A J; Gloyd, S

2016-03-01

Electronic diagnostic tests, such as the Xpert® MTB/RIF assay, are being implemented in low- and middle-income countries (LMICs). However, timely information from these tests available via remote monitoring is underutilized. The failure to transmit real-time, actionable data to key individuals such as clinicians, patients, and national monitoring and evaluation teams may negatively impact patient care. To describe recently developed applications that allow for real-time, remote monitoring of Xpert results, and initial implementation of one of these products in central Mozambique. In partnership with the Mozambican National Tuberculosis Program, we compared three different remote monitoring tools for Xpert and selected one, GxAlert, to pilot and evaluate at five public health centers in Mozambique. GxAlert software was successfully installed on all five Xpert computers, and test results are now uploaded daily via a USB internet modem to a secure online database. A password-protected web-based interface allows real-time analysis of test results, and 1200 positive tests for tuberculosis generated 8000 SMS result notifications to key individuals. Remote monitoring of diagnostic platforms is feasible in LMICs. While promising, this effort needs to address issues around patient data ownership, confidentiality, interoperability, unique patient identifiers, and data security.

Study of the Many Fluorescent Lines and the Absorption Variability in GX 301-2 with XMM-Newton

NASA Technical Reports Server (NTRS)

Fuerst, F.; Suchy, S.; Kreykenbohm, I.; Barragan, L.; Wilms, J.; Pottschmidt, K.; Caballero, I.; Kretschmar, P.; Ferrigno, C.; Rothschild, R. E.

2011-01-01

We present an in-depth study of the High Mass X-ray Binary (HMXB) GX 301-2 during its pre-periastron flare using data from the XMM-Newton satellite. The energy spectrum shows a power law continuum absorbed by a large equivalent hydrogen column on the order of 10(exp 24)/ sq cm and a prominent Fe K-alpha fluorescent emission line. Besides the Fe K-alpha line, evidence for Fe K-Beta, Ni K-alpha, Ni K-Beta, S K-alpha, Ar K-alpha, Ca K-alpha, and Cr K-alpha fluorescent lines is found. The observed line strengths are consistent with fluorescence in a cold absorber. This is the first time that Cr K-alpha is seen in emission in the X-ray spectrum of a HMXB. In addition to the modulation by the strong pulse period of approx 685 sec the source is highly variable and shows different states of activity. We perform time-resolved as well as pulse-to-pulse resolved spectroscopy to investigate differences between these states of activity. We find that fluorescent line fluxes are strongly variable and generally follow the overall flux. The N-H value is variable by a factor of 2, but not correlated to continuum normalization. We find an interval of low flux in the light curve in which the pulsations cease almost completely, without any indication of an increasing absorption column. We investigate this dip in detail and argue that it is most likely that during the dip the accretion ceased and the afterglow of the fluorescent iron accounted for the main portion of the X-ray flux. A similar dip was found earlier in RXTE data, and we compare our findings to these results.

40 CFR Appendix - Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC...

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 13 2012-07-01 2012-07-01 false Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC Manufacturing Operations Where the Standards Are Based on a 95 Percent Reduction Requirement Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL...

40 CFR Appendix - Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 12 2011-07-01 2009-07-01 true Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC Manufacturing Operations Where the Standards Are Based on a 95 Percent Reduction Requirement Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL...

40 CFR Appendix - Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC...

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 13 2013-07-01 2012-07-01 true Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC Manufacturing Operations Where the Standards Are Based on a 95 Percent Reduction Requirement Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL...

Effects of the propeptide of group X secreted phospholipase A(2) on substrate specificity and interfacial activity on phospholipid monolayers.

PubMed

Point, Vanessa; Bénarouche, Anaïs; Jemel, Ikram; Parsiegla, Goetz; Lambeau, Gérard; Carrière, Frédéric; Cavalier, Jean-François

2013-01-01

Group X secreted phospholipase A(2) (GX sPLA(2)) plays important physiological roles in the gastrointestinal tract, in immune and sperm cells and is involved in several types of inflammatory diseases. It is secreted either as a mature enzyme or as a mixture of proenzyme (with a basic 11 amino acid propeptide) and mature enzyme. The role of the propeptide in the repression of sPLA(2) activity has been studied extensively using liposomes and micelles as model interfaces. These substrates are however not always suitable for detecting some fine tuning of lipolytic enzymes. In the present study, the monolayer technique is used to compare PLA(2) activity of recombinant mouse GX sPLA(2) (mGX) and its pro-form (PromGX) on monomolecular films of dilauroyl-phosphatidyl-ethanolamine (DLPE), -choline (DLPC) and -glycerol (DLPG). The PLA(2) activity and substrate specificity of mGX (PE ≈ PG > PC) were found to be surface pressure-dependent. mGX displayed a high activity on DLPE and DLPG but not on DLPC monolayers up to surface pressures corresponding to the lateral pressure of biological membranes (30-35 mN/m). Overall, the propeptide impaired the enzyme activity, particularly on DLPE whatever the surface pressure. However some conditions could be found where the propeptide had little effects on the repression of PLA(2) activity. In particular, both PromGX and mGX had similar activities on DLPG at a surface pressure of 30 mN/m. These findings show that PromGX can be potentially active depending on the presentation of the substrate (i.e., lipid packing) and one cannot exclude such an activity in a physiological context. A structural model of PromGX was built to investigate how the propeptide controls the activity of GX sPLA(2). This model shows that the propeptide is located within the interfacial binding site (i-face) and could disrupt both the interfacial binding of the enzyme and the access to the active site by steric hindrance. Copyright © 2012 Elsevier Masson SAS

40 CFR 721.9790 - Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxypropyl) amino]- 6-[(3...

Code of Federal Regulations, 2011 CFR

2011-07-01

.... with 2,2′,2″-nitrilotris[ethanol] (1:2); Benzenesulfonic acid, 5-[[4-[bis(2-hydroxyethyl)amino]-6-[(3... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Benzenesulfonic acid, 2,2â²-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxypropyl) amino]- 6-[(3-sulfophenyl)amino]-1,3,5-triazin-2-yl]amino...

40 CFR 721.9790 - Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxypropyl) amino]- 6-[(3...

Code of Federal Regulations, 2010 CFR

2010-07-01

.... with 2,2′,2″-nitrilotris[ethanol] (1:2); Benzenesulfonic acid, 5-[[4-[bis(2-hydroxyethyl)amino]-6-[(3... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Benzenesulfonic acid, 2,2â²-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxypropyl) amino]- 6-[(3-sulfophenyl)amino]-1,3,5-triazin-2-yl]amino...

40 CFR 721.9790 - Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxypropyl) amino]- 6-[(3...

Code of Federal Regulations, 2012 CFR

2012-07-01

...]-, disodium salt, compd. with 2,2â²,2â³-nitrilo-tris[ethanol] (1:2); Benzenesulfonic acid, 5-[[4-[bis(2.... with 2,2â²,2â³-nitrilotris[ethanol] (1:2). 721.9790 Section 721.9790 Protection of Environment...]-, disodium salt, compd. with 2,2′,2″-nitrilo-tris[ethanol] (1:2); Benzenesulfonic acid, 5-[[4-[bis(2...

40 CFR 721.9790 - Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxypropyl) amino]- 6-[(3...

Code of Federal Regulations, 2014 CFR

2014-07-01

...]-, disodium salt, compd. with 2,2â²,2â³-nitrilo-tris[ethanol] (1:2); Benzenesulfonic acid, 5-[[4-[bis(2.... with 2,2â²,2â³-nitrilotris[ethanol] (1:2). 721.9790 Section 721.9790 Protection of Environment...]-, disodium salt, compd. with 2,2′,2″-nitrilo-tris[ethanol] (1:2); Benzenesulfonic acid, 5-[[4-[bis(2...

40 CFR 721.9790 - Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxypropyl) amino]- 6-[(3...

Code of Federal Regulations, 2013 CFR

2013-07-01

...]-, disodium salt, compd. with 2,2â²,2â³-nitrilo-tris[ethanol] (1:2); Benzenesulfonic acid, 5-[[4-[bis(2.... with 2,2â²,2â³-nitrilotris[ethanol] (1:2). 721.9790 Section 721.9790 Protection of Environment...]-, disodium salt, compd. with 2,2′,2″-nitrilo-tris[ethanol] (1:2); Benzenesulfonic acid, 5-[[4-[bis(2...

2 CFR 200.54 - Indian tribe (or “federally recognized Indian tribe”).

Code of Federal Regulations, 2014 CFR

2014-01-01

... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false Indian tribe (or âfederally recognized Indian tribeâ). 200.54 Section 200.54 Grants and Agreements Office of Management and Budget Guidance for... § 200.54 Indian tribe (or “federally recognized Indian tribe”). Indian tribe means any Indian tribe...

Group X phospholipase A2 is released during sperm acrosome reaction and controls fertility outcome in mice.

PubMed

Escoffier, Jessica; Jemel, Ikram; Tanemoto, Akemi; Taketomi, Yoshitaka; Payre, Christine; Coatrieux, Christelle; Sato, Hiroyasu; Yamamoto, Kei; Masuda, Seiko; Pernet-Gallay, Karin; Pierre, Virginie; Hara, Shuntaro; Murakami, Makoto; De Waard, Michel; Lambeau, Gérard; Arnoult, Christophe

2010-05-01

Ejaculated mammalian sperm must undergo a maturation process called capacitation before they are able to fertilize an egg. Several studies have suggested a role for members of the secreted phospholipase A2 (sPLA2) family in capacitation, acrosome reaction (AR), and fertilization, but the molecular nature of these enzymes and their specific roles have remained elusive. Here, we have demonstrated that mouse group X sPLA2 (mGX) is the major enzyme present in the acrosome of spermatozoa and that it is released in an active form during capacitation through spontaneous AR. mGX-deficient male mice produced smaller litters than wild-type male siblings when crossed with mGX-deficient females. Further analysis revealed that spermatozoa from mGX-deficient mice exhibited lower rates of spontaneous AR and that this was associated with decreased in vitro fertilization (IVF) efficiency due to a drop in the fertilization potential of the sperm and an increased rate of aborted embryos. Treatment of sperm with sPLA2 inhibitors and antibodies specific for mGX blocked spontaneous AR of wild-type sperm and reduced IVF success. Addition of lysophosphatidylcholine, a catalytic product of mGX, overcame these deficiencies. Finally, recombinant mGX triggered AR and improved IVF outcome. Taken together, our results highlight a paracrine role for mGX during capacitation in which the enzyme primes sperm for efficient fertilization and boosts premature AR of a likely phospholipid-damaged sperm subpopulation to eliminate suboptimal sperm from the pool available for fertilization.

Group X phospholipase A2 is released during sperm acrosome reaction and controls fertility outcome in mice

PubMed Central

Escoffier, Jessica; Jemel, Ikram; Tanemoto, Akemi; Taketomi, Yoshitaka; Payre, Christine; Coatrieux, Christelle; Sato, Hiroyasu; Yamamoto, Kei; Masuda, Seiko; Pernet-Gallay, Karin; Pierre, Virginie; Hara, Shuntaro; Murakami, Makoto; De Waard, Michel; Lambeau, Gérard; Arnoult, Christophe

2010-01-01

Ejaculated mammalian sperm must undergo a maturation process called capacitation before they are able to fertilize an egg. Several studies have suggested a role for members of the secreted phospholipase A2 (sPLA2) family in capacitation, acrosome reaction (AR), and fertilization, but the molecular nature of these enzymes and their specific roles have remained elusive. Here, we have demonstrated that mouse group X sPLA2 (mGX) is the major enzyme present in the acrosome of spermatozoa and that it is released in an active form during capacitation through spontaneous AR. mGX-deficient male mice produced smaller litters than wild-type male siblings when crossed with mGX-deficient females. Further analysis revealed that spermatozoa from mGX-deficient mice exhibited lower rates of spontaneous AR and that this was associated with decreased in vitro fertilization (IVF) efficiency due to a drop in the fertilization potential of the sperm and an increased rate of aborted embryos. Treatment of sperm with sPLA2 inhibitors and antibodies specific for mGX blocked spontaneous AR of wild-type sperm and reduced IVF success. Addition of lysophosphatidylcholine, a catalytic product of mGX, overcame these deficiencies. Finally, recombinant mGX triggered AR and improved IVF outcome. Taken together, our results highlight a paracrine role for mGX during capacitation in which the enzyme primes sperm for efficient fertilization and boosts premature AR of a likely phospholipid-damaged sperm subpopulation to eliminate suboptimal sperm from the pool available for fertilization. PMID:20424324

Novel pathogenic variant (c.3178G>A) in the SMC1A gene in a family with Cornelia de Lange syndrome identified by exome sequencing.

PubMed

Jang, Mi Ae; Lee, Chang Woo; Kim, Jin Kyung; Ki, Chang Seok

2015-11-01

Cornelia de Lange syndrome (CdLS) is a clinically and genetically heterogeneous congenital anomaly. Mutations in the NIPBL gene account for a half of the affected individuals. We describe a family with CdLS carrying a novel pathogenic variant of the SMC1A gene identified by exome sequencing. The proband was a 3-yr-old boy presenting with a developmental delay. He had distinctive facial features without major structural anomalies and tested negative for the NIPBL gene. His younger sister, mother, and maternal grandmother presented with mild mental retardation. By exome sequencing of the proband, a novel SMC1A variant, c.3178G>A, was identified, which was expected to cause an amino acid substitution (p.Glu1060Lys) in the highly conserved coiled-coil domain of the SMC1A protein. Sanger sequencing confirmed that the three female relatives with mental retardation also carry this variant. Our results reveal that SMC1A gene defects are associated with milder phenotypes of CdLS. Furthermore, we showed that exome sequencing could be a useful tool to identify pathogenic variants in patients with CdLS.

Terahertz laser spectroscopy of the water dimer intermolecular vibrations. II. (H2O)2

NASA Astrophysics Data System (ADS)

Braly, L. B.; Liu, K.; Brown, M. G.; Keutsch, F. N.; Fellers, R. S.; Saykally, R. J.

2000-06-01

Terahertz VRT laser spectra of four (H2O)2 intermolecular vibrations consisting of 362 transitions have been measured between 87 and 108 cm-1 with ca. 2 MHz precision. The results differ both qualitatively and quantitatively from the predictions of dimer potentials tested. The spectra also reveal an ordering of the intermolecular vibrations which differs dramatically from that predicted by normal mode analysis. Strong coupling is indicated between the low barrier tunneling motions and the intermolecular vibrations as well as among different vibrations. In particular the 102.1 cm-1 (H2O)2 vibration assigned as the acceptor wag (ν8) exhibits two types of perturbations. In one of these a component of Ka=1 coupling with a tunneling component of Ka=0 in the 108 cm-1 acceptor twist (ν11) vibration. There is also an indication that the 103.1 cm-1 (H2O)2 band assigned as the donor in-plane bend (ν6) is coupled to the acceptor wag resulting in a lower of the in-plane bend frequency and a higher acceptor wag frequency. Detailed analysis of the VRT levels confirms the extreme nonrigidity of this complex, indicating that the use of approximate models with reduced dimensionality to calculate its properties are likely to fail.

Identification of a Novel Enterovirus Species in Rhesus Macaque in China.

PubMed

Ao, Yuan-Yun; Yu, Jie-Mei; Zhang, Cui-Yuan; Xin, Yun-Yun; Li, Li-Li; Duan, Zhao-Jun

2016-06-22

Recent studies of Enterovirus (EV) in nonhuman primates (NHPs), which could act as a source of future emerging human viral diseases, have boosted interest in the search for novel EVs. Here, a highly divergent strain of EV, tentatively named SEV-gx, was identified by viral metagenomic analysis from stool samples of rhesus macaques in China. In total, 27 of 280 (9.6%) faecal samples from rhesus macaques were positive for SEV-gx. Its complete genomic sequence is 7,367 nucleotide (nt). Genomic analyses showed that it has a standard genomic organisation for EVs, being more closely related to EV-J strains (approximately 54.0%, 43.0-44.1%, 52.3-55.2%, 61.1-62.7% and 64.0% amino acids identity in polyprotein, P1, P2 and P3 and combined 2C/3CD regions, respectively). It was also shown to have genome characteristics typical of EVs. Phylogenetic analysis of P1, 2C and 3CD aa indicated that SEV-gx can be classified as a distinct cluster in the EVs. All of this evidence demonstrates SEV-gx is a novel species (tentatively named EV-K) in the EV genus, which contributes to our understanding of the genetic diversity and evolution of EVs. Further studies are needed to investigate the potential pathogenicity of SEV-gx in NHPs and humans.

Spectral and temporal properties of the X-ray pulsar SMC X-1 at hard X-rays

NASA Technical Reports Server (NTRS)

Kunz, M.; Gruber, D. E.; Kendziorra, E .; Kretschmar, P.; Maisack, M.; Mony, B.; Staubert, R.; Doebereiner, S.; Englhauser, J.; Pietsch, W.

1993-01-01

The binary X-ray pulsar SMC X- 1 has been observed at hard X-rays with the High Energy X-Ray Experiment (HEXE) on nine occasions between Nov. 1987 and March 1989. A thin thermal bremsstrahlung fit to the phase averaged spectrum yields a plasma temperature (14.4 +/- 1.3) keV and a luminosity above (1.1 +/- 0.1) x 10 exp 38 erg/s in the 20-80 keV band. Pulse period values have been established for three observations, confirming the remarkably stable spin-up trend of SMC X-1. In one of the three observations the pulse profile was seen to deviate from a dominant double pulsation, while at the same time the pulsed fraction was unusually large. For one observation we determined for the first time the pulsed fraction in narrow energy bands. It increases with photon energy from about 20 percent up to over 60 percent in the energy range from 20 to 80 keV.

The evolution of hydrocarbons past the asymptotic giant branch: the case of MSX SMC 029

NASA Astrophysics Data System (ADS)

Pauly, Tyler; Sloan, Gregory C.; Kraemer, Kathleen E.; Bernard-Salas, Jeronimo; Lebouteiller, Vianney; Goes, Christopher; Barry, Donald

2015-01-01

We present an optimally extracted high-resolution spectrum of MSX SMC 029 obtained by the Infrared Spectrograph on the Spitzer Space Telescope. MSX SMC 029 is a carbon-rich object in the Small Magellanic Cloud that has evolved past the asymptotic giant branch (AGB). The spectrum reveals a cool carbon-rich dust continuum with emission from polycyclic aromatic hydrocarbons (PAHs) and absorption from simpler hydrocarbons, both aliphatic and aromatic, including acetylene and benzene. The spectrum shows many similarities to the carbon-rich post-AGB objects SMP LMC 011 in the Large Magellanic Cloud and AFGL 618 in the Galaxy. Both of these objects also show infrared absorption features from simple hydrocarbons. All three spectra lack strong atomic emission lines in the infrared, indicating that we are observing the evolution of carbon-rich dust and free hydrocarbons in objects between the AGB and planetary nebulae. These three objects give us a unique view of the elusive phase when hydrocarbons exist both as relatively simple molecules and the much more complex and ubiquitous PAHs. We may be witnessing the assembly of amorphous carbon into PAHs.

Coaxial metal-silicide Ni2Si/C54-TiSi2 nanowires.

PubMed

Chen, Chih-Yen; Lin, Yu-Kai; Hsu, Chia-Wei; Wang, Chiu-Yen; Chueh, Yu-Lun; Chen, Lih-Juann; Lo, Shen-Chuan; Chou, Li-Jen

2012-05-09

One-dimensional metal silicide nanowires are excellent candidates for interconnect and contact materials in future integrated circuits devices. Novel core-shell Ni(2)Si/C54-TiSi(2) nanowires, 2 μm in length, were grown controllably via a solid-liquid-solid growth mechanism. Their interesting ferromagnetic behaviors and excellent electrical properties have been studied in detail. The coercivities (Hcs) of the core-shell Ni(2)Si/C54-TiSi(2) nanowires was determined to be 200 and 50 Oe at 4 and 300 K, respectively, and the resistivity was measured to be as low as 31 μΩ-cm. The shift of the hysteresis loop with the temperature in zero field cooled (ZFC) and field cooled (FC) studies was found. ZFC and FC curves converge near room temperature at 314 K. The favorable ferromagnetic and electrical properties indicate that the unique core-shell nanowires can be used in penetrative ferromagnetic devices at room temperature simultaneously as a future interconnection in integrated circuits.

The X-ray Halo of GX5-1

NASA Technical Reports Server (NTRS)

Smith, Randall K.; Dame, T. M.; Costantini, Elisa; Predehl, Peter

2006-01-01

Using Chandra observations we have measured the energy-resolved dust-scattered X-ray halo around the low-mass X-ray binary GX5-1, detecting for the first time multiply scattered X-rays from interstellar dust. % e compared the observed X-ray halo at various energies to predictions from a range of dust models. These fits used both smoothly-distributed dust as well as dust in clumped clouds, with CO and 21 cm observations helping to determine the position of the clouds along the line of sight. We found that the BARE-GR-B model of Zubko, Dwek & Arendt (2004) generally led to the best results, although inadequacies in both the overall model and the data limit our conclusions. We did find that the composite dust models of Zubko, Dwek & Arendt (2004), especially the "no carbon" models, gave uniformly poor results. Although models using cloud positions and densities derived naively from CO and 21 cm data gave generally poor results, plausible adjustments to the distance of the largest cloud and the mass of a cloud in the expanding 3 kpc Arm lead to significantly improved fits. We suggest that combining X-ray halo, CO, and 21 cm observations will be a fruitful method to improve our understanding of both the gas and dust phases of the interstellar medium.

THE HOST GALAXY OF THE SUPER-LUMINOUS SN 2010gx AND LIMITS ON EXPLOSIVE {sup 56}Ni PRODUCTION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chen, Ting-Wan; Smartt, Stephen J.; Kotak, Rubina

2013-02-01

Super-luminous supernovae have a tendency to occur in faint host galaxies which are likely to have low mass and low metallicity. While these extremely luminous explosions have been observed from z = 0.1 to 1.55, the closest explosions allow more detailed investigations of their host galaxies. We present a detailed analysis of the host galaxy of SN 2010gx (z = 0.23), one of the best studied super-luminous type Ic supernovae. The host is a dwarf galaxy (M{sub g} = -17.42 {+-} 0.17) with a high specific star formation rate. It has a remarkably low metallicity of 12 + log (O/H)more » = 7.5 {+-} 0.1 dex as determined from the detection of the [O III] {lambda}4363 line. This is the first reliable metallicity determination of a super-luminous stripped-envelope supernova host. We collected deep multi-epoch imaging with Gemini + GMOS between 240 and 560 days after explosion to search for any sign of radioactive {sup 56}Ni, which might provide further insights on the explosion mechanism and the progenitor's nature. We reach griz magnitudes of m{sub AB} {approx} 26, but do not detect SN 2010gx at these epochs. The limit implies that any {sup 56}Ni production was similar to or below that of SN 1998bw (a luminous type Ic SN that produced around 0.4 M{sub Sun} of {sup 56}Ni). The low volumetric rates of these supernovae ({approx}10{sup -4} of the core-collapse population) could be qualitatively matched if the explosion mechanism requires a combination of low-metallicity (below 0.2 Z{sub Sun }), high progenitor mass (>60 M{sub Sun }) and high rotation rate (fastest 10% of rotators).« less

Time-frequency dynamics during sleep spindles on the EEG in rodents with a genetic predisposition to absence epilepsy (WAG/Rij rats)

NASA Astrophysics Data System (ADS)

Hramov, Alexander E.; Sitnikova, Evgenija Y.; Pavlov, Alexey N.; Grubov, Vadim V.; Koronovskii, Alexey A.; Khramova, Marina V.

2015-03-01

Sleep spindles are known to appear spontaneously in the thalamocortical neuronal network of the brain during slow-wave sleep; pathological processes in the thalamocortical network may be the reason of the absence epilepsy. The aim of the present work is to study developed changes in the time-frequency structure of sleep spindles during the progressive development of the absence epilepsy in WAG/Rij rats. EEG recordings were made at age 7 and 9 months. Automatic recognition and subsequent analysis of sleep spindles on the EEG were performed using the continuous wavelet transform. The duration of epileptic discharges and the total duration of epileptic activity were found to increase with age, while the duration of sleep spindles, conversely, decreased. In terms of the mean frequency, sleep spindles could be divided into three classes: `slow' (mean frequency 9.3Hz), `medium' (11.4Hz), and `fast' (13.5Hz). Slow and medium (transitional) spindles in five-month-old animals showed increased frequency from the beginning to the end of the spindle. The more intense the epilepsy is, the shorter are the durations of spindles of all types. The mean frequencies of `medium' and `fast' spindles were higher in rats with more intense signs of epilepsy. Overall, high epileptic activity in WAG/Rij rats was linked with significant changes in spindles of the transitional type, with less marked changes in the two traditionally identified types of spindle, slow and fast.

Comparison of [¹¹C]choline ([¹¹C]CHO) and S(+)-β-methyl-[¹¹C]choline ([¹¹C]SMC) as imaging probes for prostate cancer in a PC-3 prostate cancer xenograft model.

PubMed

Schwarzenböck, Sarah Marie; Gertz, Jana; Souvatzoglou, Michael; Kurth, Jens; Sachs, David; Nawroth, Roman; Treiber, Uwe; Schuster, Tibor; Senekowitsch-Schmidtke, Reingard; Schwaiger, Markus; Ziegler, Sibylle Ilse; Henriksen, Gjermund; Wester, Hans-Jürgen; Krause, Bernd Joachim

2015-04-01

Carbon-11- and fluorine-18-labeled choline derivatives have been introduced as promising tracers for prostate cancer imaging. However, due to limited specificity and sensitivity, there is a need for new tracers with higher sensitivity and specificity for diagnosing prostate cancer to improve tracer uptake and enhance imaging contrast. The aim of this study was to compare the properties of [(11)C]choline ([(11)C]CHO) with S(+)-β-methyl-[(11)C]choline ([(11)C]SMC) as tracer for prostate cancer imaging in a human prostate tumor mouse xenograft model by small-animal positron emission tomography/X-ray computed tomography (PET/CT). We carried out a dual-tracer small-animal PET/CT study comparing [(11)C]CHO and [(11)C]SMC. The androgen-independent human prostate tumor cell line PC3 was implanted subcutaneously in the flanks of Naval Medical Research Institute (NMRI) (nu/nu) mice (n = 11). Mice-6 weeks post-xenograft implantation-were injected with 37 MBq [(11)C]CHO via the tail vein. On a separate day, the mice were injected with 37 MBq [(11)C]SMC. Dynamic imaging was performed for 60 min with the Inveon animal PET/CT scanner (Siemens Medical Solutions) on two separate days (randomizing the sequence of the tracers). The dynamic PET images were acquired in list mode. Regions of interest (5 × 5 × 5 mm) were placed in transaxial slices in tumor, muscle (thigh), liver, kidney, and blood. Image analysis was performed calculating tumor to muscle (T/M) ratios based on summed images as well as dynamic data. For [(11)C]SMC, the mean T/M ratio was 2.24 ± 0.56 while the corresponding mean [(11)C]CHO T/M ratio was 1.35 ± 0.28. The T/M ratio for [(11)C]SMC was significant higher compared to [(11)C]CHO (p < 0.001). The time course of T/M ratio (T/Mdyn ratio) of [(11)C]SMC was higher compared to [(11)C]CHO with a statistically significant difference between the magnitudes of the T/M ratios and a significant different change of the T/M ratios over time

Theoretical Study on Sers of Wagging Vibrations of Benzyl Radical Adsorbed on Silver Electrodes

NASA Astrophysics Data System (ADS)

Wu, De-Yin; Chen, Yan-Li; Tian, Zhong-Qun

2016-06-01

Electrochemical surface-enhanced Raman spectroscopy (EC-SERS) has been used to characterize adsorbed species widely but reaction intermediates rarely on electrodes. In previous studies, the observed SERS signals were proposed from surface benzyl species due to the electrochemical reduction of benzyl chloride on silver electrode surfaces. In this work, we reinvestigated the vibrational assignments of benzyl chloride and benzyl radical as the reaction intermediate. On the basis of density functional theoretical (DFT) calculations and normal mode analysis, our systematical results provide more reasonable new assignments for both surface species. Further, we investigated adsorption configurations, binding energies, and vibrational frequency shifts of benzyl radical interacting with silver. Our calculated results show that the wagging vibration displays significant vibrational frequency shift, strong coupling with some intramolecular modes in the phenyl ring, and significant changes in intensity of Raman signals. The study also provides absolute Raman intensity in benzyl halides and discuss the enhancement effect mainly due to the binding interaction with respect to free benzyl radical.

The U.S. Gas Flooding Experience: CO2 Injection Strategies and Impact on Ultimate Recovery

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nunez-Lopez, Vanessa; Hosseini, Seyyed; Gil-Egui, Ramon

The Permian Basin in West Texas and southwestern New Mexico has seen 45 years of oil reserve growth through CO2 enhanced oil recovery (CO2 EOR). More than 60 CO2 EOR projects are currently active in the region’s limestone, sandstone and dolomite reservoirs. Water alternating gas (WAG) has been the development strategy of choice in the Permian for several technical and economic reasons. More recently, the technology started to get implemented in the much more porous and permeable clastic depositional systems of the onshore U.S. Gulf Coast. Continued CO2 injection (CGI), as opposed to WAG, was selected as the injection strategymore » to develop Gulf Coast oil fields, where CO2 injection volumes are significantly larger (up to 6 times larger) than those of the Permian. We conducted a compositional simulation based study with the objective of comparing the CO2 utilization ratios (volume of CO2 injected to produce a barrel of oil) of 4 conventional and novel CO2 injection strategies: (1) continuous gas injection (CGI), (2) water alternating gas (WAG), (3) water curtain injection (WCI), and (4) WAG and WCI combination. These injection scenarios were simulated using the GEM module from the Computer Modeling Group (CMG). GEM is an advanced general equation-of-state compositional simulator, which includes equation of state, CO2 miscible flood, CO2/brine interactions, and complex phase behavior. The simulator is set up to model three fluid phases including water, oil, and gas. Our study demonstrates how the selected field development strategy has a significant impact on the ultimate recovery of CO2-EOR projects, with GCI injection providing maximum oil recovery in absolute volume terms, but with WAG offering a more balanced technical-economical approach.« less

Effect of filter on average glandular dose and image quality in digital mammography

NASA Astrophysics Data System (ADS)

Songsaeng, C.; Krisanachinda, A.; Theerakul, K.

2016-03-01

To determine the average glandular dose and entrance surface air kerma in both phantoms and patients to assess image quality for different target-filters (W/Rh and W/Ag) in digital mammography system. The compressed breast thickness, compression force, average glandular dose, entrance surface air kerma, peak kilovoltage and tube current time were recorded and compared between W/Rh and W/Ag target filter. The CNR and the figure of merit were used to determine the effect of target filter on image quality. The mean AGD of the W/Rh target filter was 1.75 mGy, the mean ESAK was 6.67 mGy, the mean CBT was 54.1 mm, the mean CF was 14 1bs. The mean AGD of W/Ag target filter was 2.7 mGy, the mean ESAK was 12.6 mGy, the mean CBT was 75.5 mm, the mean CF was 15 1bs. In phantom study, the AGD was 1.2 mGy at 4 cm, 3.3 mGy at 6 cm and 3.83 mGy at 7 cm thickness. The FOM was 24.6, CNR was 9.02 at thickness 6 cm. The FOM was 18.4, CNR was 8.6 at thickness 7 cm. The AGD from Digital Mammogram system with W/Rh of thinner CBT was lower than the AGD from W/Ag target filter.

Synthesis of Substituted 2,3,5,6-tetraarylbenzo(1,2-b:5,4-b')difurans

NASA Technical Reports Server (NTRS)

Abdul-Aziz, Mahmoud; Auping, Judith V.; Meador, Michael A.

1995-01-01

A series of substituted 2,3,5,6-tetraarylbenzo(l,2-b:5,4-b')difurans 1 was synthesized. This synthesis is based upon the photocyclization of 2,5-dibenzoylresorcinol dibenzyl ethers to the corresponding tetrahydrobenzo(1,2-b:5,4-b')difurans. Treatment of the photoproducts with methanesulfonyl chloride in pyridine afforded 1 in overall yields ranging from 30-72%. A number of these compounds have high fluorescence quantum yields (of phi(sub f) = 0.76-0.90), and their fluorescence spectra exhibit large solvatochromic shifts. These compounds may be suitable for use as fluorescent probes.

Youth Appraisals of Inter-Parental Conflict and Genetic and Environmental Contributions to Attention-Deficit Hyperactivity Disorder: Examination of GxE Effects in a Twin Sample

ERIC Educational Resources Information Center

Nikolas, Molly; Klump, Kelly L.; Burt, S. Alexandra

2012-01-01

Identification of gene x environment interactions (GxE) for attention-deficit hyperactivity disorder (ADHD) is a crucial component to understanding the mechanisms underpinning the disorder, as prior work indicates large genetic influences and numerous environmental risk factors. Building on prior research, children's appraisals of self-blame were…

The infiltration of experimentally induced lung metastases of colon carcinoma CC531 by adoptively transferred interleukin-2-activated natural killer cells in Wag rats.

PubMed

Kuppen, P J; Basse, P H; Goldfarb, R H; Van De Velde, C J; Fleuren, G J; Eggermont, A M

1994-02-15

The number of IL-2-activated natural killer (A-NK) cells reaching the tumor site in vivo may be crucial for their anti-tumor effect following adoptive immunotherapy. We investigated in a syngeneic rat model the infiltration of established lung metastases by adoptively transferred A-NK cells. The Wag rat colon carcinoma CC531 was injected via a tail vein to induce pulmonary metastases. Syngeneic A-NK cells were labeled with the fluorescent dye rhodamine (TRITC) and next injected via a tail vein in rats bearing day-12 lung tumors. The number of A-NK cells in tumor and in normal tissue per rat was counted in sections after administration of A-NK cells. At all time points tested, a significant linear relationship between the cross-section area of the tumor and the number of infiltrating cells was observed, but small tumor areas became fully infiltrated earlier than larger areas. At 24 hr after injection, approximately 10% of the injected cells were found in the tumor tissue and the average A-NK-cell-to-tumor-cell ratio was estimated to be 1:3. A-NK cells were found in the liver too, although the number of cells per mm2 tissue was low compared with the pulmonary tumor tissue. Very low numbers of A-NK cells were found in kidney, adrenal gland, spleen, and blood. We conclude that, in this syngeneic rat model, adoptively transferred A-NK cells are able to find and specifically infiltrate pulmonary metastases in a time-dependent fashion.

[Phylogenetic analysis of human/swine/avian gene reassortant H1N2 influenza A virus isolated from a pig in China].

PubMed

Chen, Yixiang; Meng, Xueqiong; Liu, Qi; Huang, Xia; Huang, Shengbin; Liu, Cuiquan; Shi, Kaichuang; Guo, Jiangang; Chen, Fangfang; Hu, Liping

2008-04-01

Our aim in this study was to determine the genetic characterization and probable origin of the H1N2 swine influenza virus (A/Swine/Guangxi/13/2006) (Sw/GX/13/06) from lung tissue of a pig in Guangxi province, China. Eight genes of Sw/GX/13/06 were cloned and genetically analyzed. The hemagglutinin (HA), nucleoprotein (NP), matrix (M) and non-structural (NS) genes of Sw/GX/13/06 were most closely related to genes from the classical swine H1N1 influenza virus lineage. The neuraminidase (NA) and PB1 genes were most closely related to the corresponding genes from the human influenza H3N2 virus lineage. The remaining two genes PA and PB2 polymerase genes were most closely related to the genes from avian influenza virus lineage. Phylogenetic analyses revealed that Sw/GX/13/06 was a human/swine/avian H1N2 virus, and closely related to H1N2 viruses isolated from pigs in United States (1999-2001) and Korea (2002). To our knowledge, Sw/GX/13/06 was the first triple-reassortant H1N2 influenza A virus isolated from a pig in China. Whether the Sw/GX/13/06 has a potential threat to breeding farm and human health remains to be further investigated.

The tail wags the dog: managing large telescope construction projects with lagging requirements and creeping scope

NASA Astrophysics Data System (ADS)

Warner, Mark

2014-08-01

-picture decisions early, and designing flexibility and adaptability into subsystems. In a perfect world, science would be the big dog in the room, wagging the engineering tail. In our non-perfect world, however, it's often the tail that ends up wagging the dog instead.

40 CFR 721.9798 - Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-substituted-6-substituted-1,3,5-triazin-2-yl...

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Benzenesulfonic acid, 2,2â²-(1,2-ethenediyl)bis[5-[[4-substituted-6-substituted-1,3,5-triazin-2-yl]amino]-, sodium salt (generic). 721.9798... Substances § 721.9798 Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-substituted-6-substituted-1,3,5...

40 CFR 721.9798 - Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-substituted-6-substituted-1,3,5-triazin-2-yl...

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Benzenesulfonic acid, 2,2â²-(1,2-ethenediyl)bis[5-[[4-substituted-6-substituted-1,3,5-triazin-2-yl]amino]-, sodium salt (generic). 721.9798... Substances § 721.9798 Benzenesulfonic acid, 2,2′-(1,2-ethenediyl)bis[5-[[4-substituted-6-substituted-1,3,5...

The Carnegie Hubble Program: The Distance and Structure of the SMC as Revealed by Mid-Infrared Observations of Cepheids

NASA Technical Reports Server (NTRS)

Scowcroft, Victoria; Freedman, Wendy L.; Madore, Barry F.; Monson, Andy; Persson, S. E.; Rich, Jeff; Seibert, Mark; Rigby, Jane R.

2016-01-01

Using Spitzer observations of classical Cepheids we have measured the true average distance modulus of the Small Magellanic Cloud (SMC) to be18.96 +/- 0.01 stat +/- 0.03sys mag (corresponding to 62+/- 0.3kpc), which is 0.48 +/- 0.01 mag more distant than the LMC. This is in agreement with previous results from Cepheid observations, as well as with measurements from other indicators such as RR Lyrae stars and the tip of the red giant branch. Utilizing the properties of the mid-infrared Leavitt Law we measured precise distances to individual Cepheids in the SMC, and have confirmed that the galaxy is tilted and elongated such that its eastern side is up to20 kpc closer than its western side. This is in agreement with the results from red clump stars and dynamical simulations of the Magellanic Clouds and Stream.

46 CFR 54.01-5 - Scope (modifies U-1 and U-2).

Code of Federal Regulations, 2010 CFR

2010-10-01

... (approved by the Office of Management and Budget under OMB control number 2130-0181); (2) Meet § 54.01-35... stress relief may be substituted if allowed under Subpart 54.30 of this chapter. III (a) Vapor or gas(b... pressure vessels. (Approved by the Office of Management and Budget under OMB control number 2130-0181...

46 CFR 54.01-5 - Scope (modifies U-1 and U-2).

Code of Federal Regulations, 2013 CFR

2013-10-01

... (approved by the Office of Management and Budget under OMB control number 2130-0181); (2) Meet § 54.01-35... stress relief may be substituted if allowed under subpart 54.30 of this chapter. III (a) Vapor or gas(b... pressure vessels. (Approved by the Office of Management and Budget under OMB control number 2130-0181...

46 CFR 54.01-5 - Scope (modifies U-1 and U-2).

Code of Federal Regulations, 2012 CFR

2012-10-01

... (approved by the Office of Management and Budget under OMB control number 2130-0181); (2) Meet § 54.01-35... stress relief may be substituted if allowed under Subpart 54.30 of this chapter. III (a) Vapor or gas(b... pressure vessels. (Approved by the Office of Management and Budget under OMB control number 2130-0181...

46 CFR 54.01-5 - Scope (modifies U-1 and U-2).

Code of Federal Regulations, 2011 CFR

2011-10-01

... (approved by the Office of Management and Budget under OMB control number 2130-0181); (2) Meet § 54.01-35... stress relief may be substituted if allowed under Subpart 54.30 of this chapter. III (a) Vapor or gas(b... pressure vessels. (Approved by the Office of Management and Budget under OMB control number 2130-0181...

The VLT-FLAMES survey of massive stars: mass loss and rotation of early-type stars in the SMC

NASA Astrophysics Data System (ADS)

Mokiem, M. R.; de Koter, A.; Evans, C. J.; Puls, J.; Smartt, S. J.; Crowther, P. A.; Herrero, A.; Langer, N.; Lennon, D. J.; Najarro, F.; Villamariz, M. R.; Yoon, S.-C.

2006-09-01

We have studied the optical spectra of a sample of 31 O-and early B-type stars in the Small Magellanic Cloud, 21 of which are associated with the young massive cluster NGC 346. Stellar parameters are determined using an automated fitting method (Mokiem et al. 2005, A&A, 441, 711), which combines the stellar atmosphere code FASTWIND (Puls et al. 2005, A&A, 435, 669) with the genetic algorithm based optimisation routine PIKAIA (Charbonneau 1995, ApJS, 101, 309). Comparison with predictions of stellar evolution that account for stellar rotation does not result in a unique age, though most stars are best represented by an age of 1-3 Myr. The automated method allows for a detailed determination of the projected rotational velocities. The present day v_r sin i distribution of the 21 dwarf stars in our sample is consistent with an underlying rotational velocity (v_r) distribution that can be characterised by a mean velocity of about 160 - 190 km s-1 and an effective half width of 100 - 150 km s-1. The vr distribution must include a small percentage of slowly rotating stars. If predictions of the time evolution of the equatorial velocity for massive stars within the environment of the SMC are correct (Maeder & Meynet 2001, A&A, 373, 555), the young age of the cluster implies that this underlying distribution is representative for the initial rotational velocity distribution. The location in the Hertzsprung-Russell diagram of the stars showing helium enrichment is in qualitative agreement with evolutionary tracks accounting for rotation, but not for those ignoring v_r. The mass loss rates of the SMC objects having luminosities of log L*/L⊙ ≳ 5.4 are in excellent agreement with predictions by Vink et al. (2001, A&A, 369, 574). However, for lower luminosity stars the winds are too weak to determine dot{M} accurately from the optical spectrum. Three targets were classifiedas Vz stars, two of which are located close to the theoretical zero-age main sequence. Three lower

Synthesis and Crystal and Electronic Structures of the Dinuclear Platinum Compounds [PEtPh(3)](2)[Pt(2)(&mgr;-PPh(2))(2)(C(6)F(5))(4)] and [Pt(2)(&mgr;-PPh(2))(2)(C(6)F(5))(4)]: A Computational Study by Density Functional Theory.

PubMed

Alonso, Ester; Casas, José M.; Cotton, F. Albert; Feng, Xuejung; Forniés, Juan; Fortuño, Consuelo; Tomas, Milagros

1999-11-01

The electrolytic behavior of the dinuclear complexes [NBu(4)](2)[MM'(&mgr;-PPh(2))(2)(C(6)F(5))(4)] (M = M' = Pt (1), Pd (1a); M = Pt, M' = Pd (1b)) has been studied, showing electrochemically irreversible oxidation and related reduction processes. The chemical oxidation of the binuclear compound for M = M' = Pt, results in the formation of the binuclear Pt(III) compound [Pt(2)(&mgr;-PPh(2))(2)(C(6)F(5))(4)]. The crystal structure analysis of both complexes has been carried out, showing very similar structures with similar Pt-C and Pt-P distances and analogous skeletons. However the Pt-Pt distances are very different, 3.621(1) Å for the Pt(II) compound and 2.7245(7) Å for the Pt(III) derivative (as are the parameters geometrically related to this Pt-Pt distance), suggesting that, in the Pt(III) compound, there is a strong Pt-Pt bond. Results of DFT calculations on [Pt(2)(&mgr;-PH(2))(2)(C(6)F(5))(4)](n)()(-) (n = 2, 0) agree very well with the crystallographic data and indicate that, in the Pt(III) compound, there is approximately a single sigma bond between the metal atoms.

Multiple facets of sialomucin complex/MUC4, a membrane mucin and erbb2 ligand, in tumors and tissues (Y2K update).

PubMed

Carraway, K L; Price-Schiavi, S A; Komatsu, M; Idris, N; Perez, A; Li, P; Jepson, S; Zhu, X; Carvajal, M E; Carraway, C A

2000-01-01

Sialomucin complex (SMC, MUC4) is a high Mr glycoprotein heterodimer, composed of mucin (ASGP-1) and transmembrane (ASGP-2) subunits. ASGP-2 contains two EGF-like domains and acts as an intramembrane ligand for the receptor tyrosine kinase ErbB2. Transfection studies with SMC DNAs showed that SMC expression could markedly reduce both cell-cell and cell-matrix interactions in vitro and increase the growth of primary tumors and the formation of metastatic foci of human A375 melanoma cells as xenotransplants in nude mice, possibly through the ability to suppress apoptosis. SMC is expressed in most vulnerable epithelia as a protective agent, which is found in both membrane and soluble forms at luminal surfaces and secreted into fluids such as milk and tears. SMC appears to be constitutively expressed by most accessible epithelia, notable exceptions being the mammary gland and uterine luminal epithelium, in which it is tightly regulated during pregnancy. Down-regulation at the luminal uterine surface appears necessary for blastocyst implantation. TGF-b is a potent repressor of SMC expression in the mammary gland and uterus, though by different mechanisms. These combined results suggest that SMC has multiple functions in epithelia and is tightly regulated in those tissues where its special functions are required.

Terahertz laser spectroscopy of the water dimer intermolecular vibrations. II. (H{sub 2}O){sub 2}

DOE Office of Scientific and Technical Information (OSTI.GOV)

Braly, L. B.; Liu, K.; Brown, M. G.

Terahertz VRT laser spectra of four (H{sub 2}O){sub 2} intermolecular vibrations consisting of 362 transitions have been measured between 87 and 108 cm{sup -1} with ca. 2 MHz precision. The results differ both qualitatively and quantitatively from the predictions of dimer potentials tested. The spectra also reveal an ordering of the intermolecular vibrations which differs dramatically from that predicted by normal mode analysis. Strong coupling is indicated between the low barrier tunneling motions and the intermolecular vibrations as well as among different vibrations. In particular the 102.1 cm{sup -1} (H{sub 2}O){sub 2} vibration assigned as the acceptor wag ({nu}{sub 8})more » exhibits two types of perturbations. In one of these a component of K{sub a}=1 coupling with a tunneling component of K{sub a}=0 in the 108 cm{sup -1} acceptor twist ({nu}{sub 11}) vibration. There is also an indication that the 103.1 cm{sup -1} (H{sub 2}O){sub 2} band assigned as the donor in-plane bend ({nu}{sub 6}) is coupled to the acceptor wag resulting in a lower of the in-plane bend frequency and a higher acceptor wag frequency. Detailed analysis of the VRT levels confirms the extreme nonrigidity of this complex, indicating that the use of approximate models with reduced dimensionality to calculate its properties are likely to fail. (c) 2000 American Institute of Physics.« less

41 CFR 301-54.2 - What is disposable pay?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 41 Public Contracts and Property Management 4 2010-07-01 2010-07-01 false What is disposable pay... BILLED TRAVEL CHARGE CARD General Rules § 301-54.2 What is disposable pay? Disposable pay is your..., etc. Deductions may be made from any type of pay you receive from your agency, e.g., basic pay...

28 CFR 54.310 - Recruitment.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Recruitment. 54.310 Section 54.310 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... §§ 54.300 through 54.310 apply shall not recruit primarily or exclusively at educational institutions...

Self-consistent physical parameters for five intermediate-age SMC stellar clusters from CMD modelling

NASA Astrophysics Data System (ADS)

Dias, B.; Kerber, L. O.; Barbuy, B.; Santiago, B.; Ortolani, S.; Balbinot, E.

2014-01-01

Context. Stellar clusters in the Small Magellanic Cloud (SMC) are useful probes for studying the chemical and dynamical evolution of this neighbouring dwarf galaxy, enabling inspection of a large period covering over 10 Gyr. Aims: The main goals of this work are the derivation of age, metallicity, distance modulus, reddening, core radius, and central density profiles for six sample clusters, in order to place them in the context of the Small Cloud evolution. The studied clusters are AM 3, HW 1, HW 34, HW 40, Lindsay 2, and Lindsay 3; HW 1, HW 34, and Lindsay 2 are studied for the first time. Methods: Optical colour-magnitude diagrams (V,B - V CMDs) and radial density profiles were built from images obtained with the 4.1 m Southern Astrophysical Research (SOAR) telescope, reaching V ~ 23. The determination of structural parameters were carried out by applying King profile fitting. The other parameters were derived in a self-consistent way by means of isochrone fitting, which uses likelihood statistics to identify the synthetic CMDs that best reproduce the observed ones. Membership probabilities were determined comparing the cluster and control field CMDs. Completeness and photometric uncertainties were obtained by performing artificial star tests. Results: The results confirm that these clusters (except HW 34, identified as a field fluctuation) are intermediate-age clusters, with ages between 1.2 Gyr (Lindsay 3) and ~5.0 Gyr (HW 1). In particular HW 1, Lindsay 2 and Lindsay 3 are located in a region that we called West Halo, where studies of ages and metallicity gradients are still lacking. Moreover, Lindsay 2 was identified as a moderately metal-poor cluster with [Fe/H] = -1.4 ± 0.2 dex, lower than expected from the age-metallicity relation by Pagel & Tautvaisiene (1998). We also found distances varying from ~53 kpc to 66 kpc, compatible with the large depth of the SMC. Based on observations obtained at the Southern Astrophysical Research (SOAR) telescope, which

Molecular and functional characterization of polymorphisms in the secreted phospholipase A2 group X gene: relevance to coronary artery disease.

PubMed

Gora, Sarah; Perret, Claire; Jemel, Ikram; Nicaud, Viviane; Lambeau, Gérard; Cambien, François; Ninio, Ewa; Blankenberg, Stefan; Tiret, Laurence; Karabina, Sonia-Athina

2009-07-01

Among secreted phospholipases A2 (sPLA2s), human group X sPLA2 (hGX sPLA2) is emerging as a novel attractive therapeutic target due to its implication in inflammatory diseases. To elucidate whether hGX sPLA2 plays a causative role in coronary artery disease (CAD), we screened the human PLA2G10 gene to identify polymorphisms and possible associations with CAD end-points in a prospective study, AtheroGene. We identified eight polymorphisms, among which, one non-synonymous polymorphism R38C in the propeptide region of the sPLA2. The T-512C polymorphism located in the 5' untranslated region was associated with a decreased risk of recurrent cardiovascular events during follow-up. The functional analysis of the R38C polymorphism showed that it leads to a profound change in expression and activity of hGX sPLA2, although there was no detectable impact on CAD risk. Due to the potential role of hGX sPLA2 in inflammatory processes, these polymorphisms should be investigated in other inflammatory diseases.

10 CFR 54.27 - Hearings.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 2 2011-01-01 2011-01-01 false Hearings. 54.27 Section 54.27 Energy NUCLEAR REGULATORY... Provisions § 54.27 Hearings. A notice of an opportunity for a hearing will be published in the Federal Register in accordance with 10 CFR 2.105. In the absence of a request for a hearing filed within 30 days by...

Intestinal fatty acid binding protein Ala54Thr polymorphism is associated with peripheral atherosclerosis combined with type 2 diabetes mellitus.

PubMed

Khattab, Salma A; Abo-Elmatty, Dina M; Ghattas, Maivel H; Mesbah, Noha M; Mehanna, Eman T

2017-09-01

Intestinal fatty acid-binding protein 2 (FABP2) is expressed in enterocytes and binds saturated and unsaturated long-chain fatty acids. The FABP2 Ala54Thr polymorphism has been reported to effect lipid metabolism. The aim of the present study was to assess the relationship between this polymorphism and peripheral atherosclerosis combined with type 2 diabetes mellitus (T2DM) in an Egyptian population. The study was performed on 100 T2DM patients with peripheral atherosclerosis and 100 control subjects. The Ala54Thr polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism, whereas serum FABP2 levels were determined using ELISA. Fasting blood glucose, fasting serum insulin concentrations, HbA1c, lipid profile, body mass index (BMI) and systolic and diastolic blood pressure (SBP and DBP, respectively) were determined. There was a higher frequency of the Thr54 allele among the patient group (P = 0.002). In Ala54/Thr54 heterozygotes and carriers of the rare Thr54/Thr54 genotype, there were significant increases in BMI and FABP2. Those with the Thr54/Thr54 genotype had significantly decreased high-density lipoprotein cholesterol (HDL-C) concentrations; in addition, those with the Thr54/Thr54 genotype had significantly higher SBP and DBP than subjects with the Ala54/Ala54 and Ala54/Thr54 genotypes. There was a positive correlation between FABP2 levels and BMI, SBP and DBP, and a negative correlation with HDL-C. The Thr54 allele of the FABP2 Ala54Thr polymorphism was associated with an increased incidence of peripheral atherosclerosis combined with T2DM in the population studied. © 2016 Ruijin Hospital, Shanghai Jiaotong University School of Medicine and John Wiley & Sons Australia, Ltd.

Unlike type 2 diabetes, type 1 does not interact with the codon 54 polymorphism of the fatty acid binding protein 2 gene.

PubMed

Georgopoulos, Angeliki; Aras, Omer; Noutsou, Marina; Tsai, Michael Y

2002-08-01

In type 2 diabetes, the threonine (Thr) for alanine (Ala) codon 54 polymorphism of the fatty acid binding protein 2 gene is associated with elevated fasting and postprandial triglycerides and dyslipidemia when compared with the wild type (Ala-54/Ala-54). To assess whether this is the case in patients with type 1 diabetes, who usually do not manifest the metabolic syndrome, we screened 181 patients with similar glycemic control as the type 2 patients. Thirty percent were heterozygous, and 9% were homozygous for the polymorphism. Mean (+/-SEM) fasting plasma triglyceride levels in patients with the wild type (n = 84), those heterozygous for Ala-54/Thr-54 (n = 44), and those homozygous for the Thr-54 (n = 13) were 1.0 +/- 0.07, 1.1 +/- 0.17, and 1.2 +/- 0.23 mmol/liter, respectively. In addition, there were no differences in total, low-density lipoprotein, high-density lipoprotein, and non-high density lipoprotein cholesterol among the three groups. After a fat load, the postprandial area under the curve of triglyceride in plasma, chylomicrons, and very low-density lipoprotein were similar between the wild type (n = 18) and the Thr-54 homozygotes (n = 12). In conclusion, in contrast to type 2, type 1 diabetes does not interact with the codon 54 polymorphism of the fatty acid binding protein 2 gene to cause hypertriglyceridemia/dyslipidemia. Insulin resistance could account possibly for this difference.

Multi-Target State Extraction for the SMC-PHD Filter

PubMed Central

Si, Weijian; Wang, Liwei; Qu, Zhiyu

2016-01-01

The sequential Monte Carlo probability hypothesis density (SMC-PHD) filter has been demonstrated to be a favorable method for multi-target tracking. However, the time-varying target states need to be extracted from the particle approximation of the posterior PHD, which is difficult to implement due to the unknown relations between the large amount of particles and the PHD peaks representing potential target locations. To address this problem, a novel multi-target state extraction algorithm is proposed in this paper. By exploiting the information of measurements and particle likelihoods in the filtering stage, we propose a validation mechanism which aims at selecting effective measurements and particles corresponding to detected targets. Subsequently, the state estimates of the detected and undetected targets are performed separately: the former are obtained from the particle clusters directed by effective measurements, while the latter are obtained from the particles corresponding to undetected targets via clustering method. Simulation results demonstrate that the proposed method yields better estimation accuracy and reliability compared to existing methods. PMID:27322274

28 CFR 54.510 - Recruitment.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Recruitment. 54.510 Section 54.510 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... in Employment in Education Programs or Activities Prohibited § 54.510 Recruitment. (a...

28 CFR 54.510 - Recruitment.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Recruitment. 54.510 Section 54.510 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... in Employment in Education Programs or Activities Prohibited § 54.510 Recruitment. (a...

28 CFR 54.200 - Application.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Application. 54.200 Section 54.200 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Coverage § 54.200 Application...

Design and analysis of a 3D-flux flux-switching permanent magnet machine with SMC cores and ferrite magnets

NASA Astrophysics Data System (ADS)

Liu, Chengcheng; Wang, Youhua; Lei, Gang; Guo, Youguang; Zhu, Jianguo

2017-05-01

Since permanent magnets (PM) are stacked between the adjacent stator teeth and there are no windings or PMs on the rotor, flux-switching permanent magnet machine (FSPMM) owns the merits of good flux concentrating and robust rotor structure. Compared with the traditional PM machines, FSPMM can provide higher torque density and better thermal dissipation ability. Combined with the soft magnetic composite (SMC) material and ferrite magnets, this paper proposes a new 3D-flux FSPMM (3DFFSPMM). The topology and operation principle are introduced. It can be found that the designed new 3DFFSPMM has many merits over than the traditional FSPMM for it can utilize the advantages of SMC material. Moreover, the PM flux of this new motor can be regulated by using the mechanical method. 3D finite element method (FEM) is used to calculate the magnetic field and parameters of the motor, such as flux density, inductance, PM flux linkage and efficiency map. The demagnetization analysis of the ferrite magnet is also addressed to ensure the safety operation of the proposed motor.

28 CFR 54.540 - Advertising.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Advertising. 54.540 Section 54.540... in Employment in Education Programs or Activities Prohibited § 54.540 Advertising. A recipient shall not in any advertising related to employment indicate preference, limitation, specification, or...

28 CFR 54.540 - Advertising.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Advertising. 54.540 Section 54.540... in Employment in Education Programs or Activities Prohibited § 54.540 Advertising. A recipient shall not in any advertising related to employment indicate preference, limitation, specification, or...

28 CFR 54.540 - Advertising.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Advertising. 54.540 Section 54.540... in Employment in Education Programs or Activities Prohibited § 54.540 Advertising. A recipient shall not in any advertising related to employment indicate preference, limitation, specification, or...

28 CFR 54.540 - Advertising.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Advertising. 54.540 Section 54.540... in Employment in Education Programs or Activities Prohibited § 54.540 Advertising. A recipient shall not in any advertising related to employment indicate preference, limitation, specification, or...

28 CFR 54.540 - Advertising.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Advertising. 54.540 Section 54.540... in Employment in Education Programs or Activities Prohibited § 54.540 Advertising. A recipient shall not in any advertising related to employment indicate preference, limitation, specification, or...

28 CFR 54.510 - Recruitment.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Recruitment. 54.510 Section 54.510... in Employment in Education Programs or Activities Prohibited § 54.510 Recruitment. (a) Nondiscriminatory recruitment and hiring. A recipient shall not discriminate on the basis of sex in the recruitment...

The optical light curve of the low-mass X-ray binary GX 9 + 9

NASA Technical Reports Server (NTRS)

Schaefer, Bradley E.

1990-01-01

The detection of a small modulation in the light curve of the GX 9 + 9 optical counterpart at the same period as determined from the X-ray data is reported. The optical variability is roughly sinusoidal in shape with a period of 4.198 + or - 0.0094 hours and an average peak-to-peak amplitude in the B of 0.19 mag with comparable amplitudes in the V and R bandpasses, and has superposed flickering with a typical amplitude of six percent. The mass of the companion star is deduced to be 0.4 solar mass, which corresponds to an early M-type star. The bulk of the optical light arises in the accretion disk, while the variability arises from orbital modulation of the light reprocessed off the companion star and a bright spot. It is suggested that the X-ray modulation might be due to the asymmetries of X-rays reflected off the bright spot.

15 CFR 400.54 - Information.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Information. 400.54 Section 400.54..., Notice, Hearings and Information § 400.54 Information. (a) Request for information. The Executive Secretary, on behalf of the Board, may request submission of any information, including business proprietary...

15 CFR 400.54 - Information.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Information. 400.54 Section 400.54..., Notice, Hearings and Information § 400.54 Information. (a) Request for information. The Executive Secretary, on behalf of the Board, may request submission of any information, including business proprietary...

SMC 1 or What's in a Name?

NASA Astrophysics Data System (ADS)

Dickel, H. R.

What's in a name? everything! SMC 1 is a planetary nebula in the Large Magellanic Cloud! This new planetary nebula near the LMC was noted by Savage, Murdin and Clark (in The Observatory 1982); it is also known as SMP LMC 104A (Sanduleak, MacConnell, and Philip in PASP 1978). In an effort to promote clear and unambiguous identification of all astronomical objects outside the solar system, the IAU Task Group on Designations attempts to clarify existing astronomical designations and the TG reviews, updates, and advertises the IAU Recommendations for Nomenclature. The following documents on the Web are provided as a service to astronomers to help them with designating astronomical sources of radiation outside the solar system: How to refer to a source or designate a new one: instructions IAU Recommendations for Nomenclature: nomenclature Second Reference Dictionary of Nomenclature of Celestial Objects: dictionary **NEW** (pre-)Registry of New Acronyms: acronym registry The Task Group in collaboration with several editors of astronomical journals and managers of large data archives is now studying the feasibility of an automated system to detect nonconforming designations when an article and/or survey data are submitted for publication and/or to an electronic archive. H. Dickel is available during the Symposium to discuss your designation concerns and to offer possible solutions.

37 CFR 2.54 - Requirements for drawings submitted on paper.

Code of Federal Regulations, 2010 CFR

2010-07-01

... OFFICE, DEPARTMENT OF COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Drawing § 2.54 Requirements for... black ink, or in color if color is claimed as a feature of the mark. (e) Drawings must be typed or made with a pen or by a process that will provide high definition when copied. A photolithographic, printer...

Group X Phospholipase A2 Stimulates the Proliferation of Colon Cancer Cells by Producing Various Lipid Mediators

PubMed Central

Surrel, Fanny; Jemel, Ikram; Boilard, Eric; Bollinger, James G.; Payré, Christine; Mounier, Carine M.; Talvinen, Kati A.; Laine, Veli J. O.; Nevalainen, Timo J.; Gelb, Michael H.

2009-01-01

Among mammalian secreted phospholipases A2 (sPLA2s), the group X enzyme has the most potent hydrolyzing capacity toward phosphatidylcholine, the major phospholipid of cell membrane and lipoproteins. This enzyme has recently been implicated in chronic inflammatory diseases such as atherosclerosis and asthma and may also play a role in colon tumorigenesis. We show here that group X sPLA2 [mouse (m)GX] is one of the most highly expressed PLA2 in the mouse colon and that recombinant mouse and human enzymes stimulate proliferation and mitogen-activated protein kinase activation of various colon cell lines, including Colon-26 cancer cells. Among various recombinant sPLA2s, mGX is the most potent enzyme to stimulate cell proliferation. Based on the use of sPLA2 inhibitors, catalytic site mutants, and small interfering RNA silencing of cytosolic PLA2α and M-type sPLA2 receptor, we demonstrate that mGX promotes cell proliferation independently of the receptor and via its intrinsic catalytic activity and production of free arachidonic acid and lysophospholipids, which are mitogenic by themselves. mGX can also elicit the production of large amounts of prostaglandin E2 and other eicosanoids from Colon-26 cells, but these lipid mediators do not play a role in mGX-induced cell proliferation because inhibitors of cyclooxygenases and lipoxygenases do not prevent sPLA2 mitogenic effects. Together, our results indicate that group X sPLA2 may play an important role in colon tumorigenesis by promoting cancer cell proliferation and releasing various lipid mediators involved in other key events in cancer progression. PMID:19602573

Group X phospholipase A2 stimulates the proliferation of colon cancer cells by producing various lipid mediators.

PubMed

Surrel, Fanny; Jemel, Ikram; Boilard, Eric; Bollinger, James G; Payré, Christine; Mounier, Carine M; Talvinen, Kati A; Laine, Veli J O; Nevalainen, Timo J; Gelb, Michael H; Lambeau, Gérard

2009-10-01

Among mammalian secreted phospholipases A2 (sPLA(2)s), the group X enzyme has the most potent hydrolyzing capacity toward phosphatidylcholine, the major phospholipid of cell membrane and lipoproteins. This enzyme has recently been implicated in chronic inflammatory diseases such as atherosclerosis and asthma and may also play a role in colon tumorigenesis. We show here that group X sPLA(2) [mouse (m)GX] is one of the most highly expressed PLA(2) in the mouse colon and that recombinant mouse and human enzymes stimulate proliferation and mitogen-activated protein kinase activation of various colon cell lines, including Colon-26 cancer cells. Among various recombinant sPLA(2)s, mGX is the most potent enzyme to stimulate cell proliferation. Based on the use of sPLA(2) inhibitors, catalytic site mutants, and small interfering RNA silencing of cytosolic PLA(2)alpha and M-type sPLA(2) receptor, we demonstrate that mGX promotes cell proliferation independently of the receptor and via its intrinsic catalytic activity and production of free arachidonic acid and lysophospholipids, which are mitogenic by themselves. mGX can also elicit the production of large amounts of prostaglandin E2 and other eicosanoids from Colon-26 cells, but these lipid mediators do not play a role in mGX-induced cell proliferation because inhibitors of cyclooxygenases and lipoxygenases do not prevent sPLA(2) mitogenic effects. Together, our results indicate that group X sPLA(2) may play an important role in colon tumorigenesis by promoting cancer cell proliferation and releasing various lipid mediators involved in other key events in cancer progression.

40 CFR Table 5 to Subpart Wwww of... - Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC...

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 13 2014-07-01 2014-07-01 false Alternative Organic HAP Emissions Limits for Open Molding, Centrifugal Casting, and SMC Manufacturing Operations Where the Standards Are Based on a 95 Percent Reduction Requirement 5 Table 5 to Subpart WWWW of Part 63 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED)...

The Cohesion Protein SOLO Associates with SMC1 and Is Required for Synapsis, Recombination, Homolog Bias and Cohesion and Pairing of Centromeres in Drosophila Meiosis

PubMed Central

Yan, Rihui; McKee, Bruce D.

2013-01-01

Cohesion between sister chromatids is mediated by cohesin and is essential for proper meiotic segregation of both sister chromatids and homologs. solo encodes a Drosophila meiosis-specific cohesion protein with no apparent sequence homology to cohesins that is required in male meiosis for centromere cohesion, proper orientation of sister centromeres and centromere enrichment of the cohesin subunit SMC1. In this study, we show that solo is involved in multiple aspects of meiosis in female Drosophila. Null mutations in solo caused the following phenotypes: 1) high frequencies of homolog and sister chromatid nondisjunction (NDJ) and sharply reduced frequencies of homolog exchange; 2) reduced transmission of a ring-X chromosome, an indicator of elevated frequencies of sister chromatid exchange (SCE); 3) premature loss of centromere pairing and cohesion during prophase I, as indicated by elevated foci counts of the centromere protein CID; 4) instability of the lateral elements (LE)s and central regions of synaptonemal complexes (SCs), as indicated by fragmented and spotty staining of the chromosome core/LE component SMC1 and the transverse filament protein C(3)G, respectively, at all stages of pachytene. SOLO and SMC1 are both enriched on centromeres throughout prophase I, co-align along the lateral elements of SCs and reciprocally co-immunoprecipitate from ovarian protein extracts. Our studies demonstrate that SOLO is closely associated with meiotic cohesin and required both for enrichment of cohesin on centromeres and stable assembly of cohesin into chromosome cores. These events underlie and are required for stable cohesion of centromeres, synapsis of homologous chromosomes, and a recombination mechanism that suppresses SCE to preferentially generate homolog crossovers (homolog bias). We propose that SOLO is a subunit of a specialized meiotic cohesin complex that mediates both centromeric and axial arm cohesion and promotes homolog bias as a component of chromosome

The cohesion protein SOLO associates with SMC1 and is required for synapsis, recombination, homolog bias and cohesion and pairing of centromeres in Drosophila Meiosis.

PubMed

Yan, Rihui; McKee, Bruce D

2013-01-01

Cohesion between sister chromatids is mediated by cohesin and is essential for proper meiotic segregation of both sister chromatids and homologs. solo encodes a Drosophila meiosis-specific cohesion protein with no apparent sequence homology to cohesins that is required in male meiosis for centromere cohesion, proper orientation of sister centromeres and centromere enrichment of the cohesin subunit SMC1. In this study, we show that solo is involved in multiple aspects of meiosis in female Drosophila. Null mutations in solo caused the following phenotypes: 1) high frequencies of homolog and sister chromatid nondisjunction (NDJ) and sharply reduced frequencies of homolog exchange; 2) reduced transmission of a ring-X chromosome, an indicator of elevated frequencies of sister chromatid exchange (SCE); 3) premature loss of centromere pairing and cohesion during prophase I, as indicated by elevated foci counts of the centromere protein CID; 4) instability of the lateral elements (LE)s and central regions of synaptonemal complexes (SCs), as indicated by fragmented and spotty staining of the chromosome core/LE component SMC1 and the transverse filament protein C(3)G, respectively, at all stages of pachytene. SOLO and SMC1 are both enriched on centromeres throughout prophase I, co-align along the lateral elements of SCs and reciprocally co-immunoprecipitate from ovarian protein extracts. Our studies demonstrate that SOLO is closely associated with meiotic cohesin and required both for enrichment of cohesin on centromeres and stable assembly of cohesin into chromosome cores. These events underlie and are required for stable cohesion of centromeres, synapsis of homologous chromosomes, and a recombination mechanism that suppresses SCE to preferentially generate homolog crossovers (homolog bias). We propose that SOLO is a subunit of a specialized meiotic cohesin complex that mediates both centromeric and axial arm cohesion and promotes homolog bias as a component of chromosome

The predicted luminous satellite populations around SMC- and LMC-mass galaxies - a missing satellite problem around the LMC?

NASA Astrophysics Data System (ADS)

Dooley, Gregory A.; Peter, Annika H. G.; Carlin, Jeffrey L.; Frebel, Anna; Bechtol, Keith; Willman, Beth

2017-11-01

Recent discovery of many dwarf satellite galaxies in the direction of the Small and Large Magellanic Clouds (SMC and LMC) provokes questions of their origins, and what they can reveal about galaxy evolution theory. Here, we predict the satellite stellar mass function of Magellanic Cloud-mass host galaxies using abundance matching and reionization models applied to the Caterpillar simulations. Specifically focusing on the volume within 50 kpc of the LMC, we predict a mean of four to eight satellites with stellar mass M* > 104 M⊙, and three to four satellites with 80 < M* ≤ 3000 M⊙. Surprisingly, all 12 currently known satellite candidates have stellar masses of 80 < M* ≤ 3000 M⊙. Reconciling the dearth of large satellites and profusion of small satellites is challenging and may require a combination of a major modification of the M*-Mhalo relationship (steep, but with an abrupt flattening at 103 M⊙), late reionization for the Local Group (zreion ≲ 9 preferred) and/or strong tidal stripping. We can more robustly predict that ∼53 per cent of satellites within this volume were accreted together with the LMC and SMC and ∼47 per cent were only ever Milky Way satellites. Observing satellites of isolated LMC-sized field galaxies is essential to place the LMC in context, and to better constrain the M*-Mhalo relationship. Modelling known LMC-sized galaxies within 8 Mpc, we predict 1-6 (2-12) satellites with M* > 105 M⊙ (M* > 104 M⊙) within the virial volume of each, and 1-3 (1-7) within a single 1.5° diameter field of view, making their discovery likely.

Influence of ALA54THR polymorphism of fatty acid binding protein 2 on lifestyle modification response in obese subjects.

PubMed

de Luis, D A; Aller, R; Izaola, O; Sagrado, M Gonzalez; Conde, R

2006-01-01

It has been found that the expression of fatty acid binding protein 2 (FABP2) mRNA is under dietary control. A G-to-A transition at codon 54 of FABP2 results in an amino acid substitution (from Ala 54 to Thr 54). This polymorphism was associated with high insulin resistance and high fasting insulin concentrations. The aim of our study was to investigate the influence of Thr54 polymorphism in the FABP2 protein on the response to a lifestyle modification (Mediterranean hypocaloric diet and exercise) in obese patients. A population of 69 obese (body mass index > 30) nondiabetic outpatients was analyzed in a prospective way. Before and after 3 months of the lifestyle modification program, indirect calorimetry, tetrapolar electrical bioimpedance measurement, blood pressure recording, serial assessment of the nutritional intake (3 days of written food records), and biochemical analysis were performed. The lifestyle modification program consisted of a hypocaloric diet (1,520 kcal; 52% carbohydrates, 25% lipids, and 23% proteins). The exercise program consisted of aerobic exercise for at least three times/week (60 min each). Statistical analysis was performed for combined Ala54/Thr54 and Thr54/Thr54 as a mutant group and wild-type Ala54/Ala54 as second group. The mean age was 45.5 +/- 16.7 years, the mean body mass index was 34.1 +/- 5.1, and there were 14 males (20.3%) and 55 females (79.7%) with a weight loss of 3.17 +/- 3.5 kg (3.5%). Thirty-seven patients (53.6%) had the genotype Ala54/Ala54 (wild-type group) and 32 (46.4%) patients either the genotype Ala54/Thr54 (26 patients, 30.2%) or the genotype Thr54/Thr54 (6 patients, 16.2%). The percentage of responders (weight loss) was similar in both groups (89.2 vs. 90.6%). In the wild-type group, body mass index, weight, fat mass, low-density lipoprotein cholesterol level, and waist circumference decreased, whereas the VO2 (oxygen consumption) increased. In the mutant group, glucose, body mass index, weight, waist

40 CFR 721.5262 - 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4...

Code of Federal Regulations, 2011 CFR

2011-07-01

... identified generically as 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2... 40 Protection of Environment 31 2011-07-01 2011-07-01 false 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(1-sulfo-2-naphthalenyl)azo...

40 CFR 721.5262 - 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4...

Code of Federal Regulations, 2010 CFR

2010-07-01

... identified generically as 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2... 40 Protection of Environment 30 2010-07-01 2010-07-01 false 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(1-sulfo-2-naphthalenyl)azo...

Health and safety plan for the Remedial Investigation and Site Investigation of Waste Area Grouping 2 at the Oak Ridge National Laboratory, Oak Ridge, Tennessee. Environmental Restoration Program

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cofer, G.H.; Holt, V.L.; Roupe, G.W.

1993-11-01

This health and safety plan (HASP) was developed by the members of the Measurement Applications and Development Group of the Health Science Research Division at the Oak Ridge National Laboratory (ORNL). This plan was prepared to ensure that health and safety related items for the Waste Area Grouping (WAG) 2 Remedial Investigation (RI)/Feasibility Study and Site Investigation projects conform with the requirements of 29 CFR 1910.120 (April 18, 1992). The RI Plan calls for the characterization, monitoring, risk assessment, and identification of remedial needs and alternatives that have been structured and staged with short-term and long-term objectives. In early FYmore » 1992, the WAG 2 RI was integrated with the ORNL Environmental Restoration (ER) Site Investigations program in order to achieve the complimentary objectives of the projects more effectively by providing an integrated basis of support. The combined effort was named the WAG 2 Remedial Investigation and Site Investigations Program (WAG 2 RI&SI). The Site Investigation activities are a series of monitoring efforts and directed investigations that support other ER activities by providing information about (1) watershed hydrogeology; (2) contaminants, pathways, and fluxes for groundwater at ORNL; (3) shallow subsurface areas that can act as secondary sources of contaminants; and (4) biological populations and contaminants in biota, in addition to other support and coordination activities.« less

47 CFR 27.54 - Frequency stability.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false Frequency stability. 27.54 Section 27.54 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS COMMUNICATIONS SERVICES Technical Standards § 27.54 Frequency stability. The frequency stability shall be...

47 CFR 27.54 - Frequency stability.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 2 2011-10-01 2011-10-01 false Frequency stability. 27.54 Section 27.54 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES MISCELLANEOUS WIRELESS COMMUNICATIONS SERVICES Technical Standards § 27.54 Frequency stability. The frequency stability shall be...

Graduate Course Contract Law Deskbook (54th). Volume 2

DTIC Science & Technology

2005-01-01

GRADUATE COURSE THE FIFTY-FOURTH CONTRACT LAW DESKBOOK Contract and Fiscal Law Department The Judge Advocate General’s School, United States Army...OF ABSTRACT 8. 54th GC Contract Law Deskbook, Volume II, Fall 2005 1. AGENCY USE ONLY (Leave blank) 4. TITLE AND SUBTITLE 54th Graduate...Louisiana, 1998-1999; Contract Law Attorney, and Chief, Administrative Law, Office of the Staff Judge Advocate, Fort Polk, Louisiana, 1996-1998. Member

Virtual Visualisation Laboratory for Science and Mathematics Content (Vlab-SMC) with Special Reference to Teaching and Learning of Chemistry

NASA Astrophysics Data System (ADS)

Badioze Zaman, Halimah; Bakar, Norashiken; Ahmad, Azlina; Sulaiman, Riza; Arshad, Haslina; Mohd. Yatim, Nor Faezah

Research on the teaching of science and mathematics in schools and universities have shown that available teaching models are not effective in instilling the understanding of scientific and mathematics concepts, and the right scientific and mathematics skills required for learners to become good future scientists (mathematicians included). The extensive development of new technologies has a marked influence on education, by facilitating the design of new learning and teaching materials, that can improve the attitude of learners towards Science and Mathematics and the plausibility of advanced interactive, personalised learning process. The usefulness of the computer in Science and Mathematics education; as an interactive communication medium that permits access to all types of information (texts, images, different types of data such as sound, graphics and perhaps haptics like smell and touch); as an instrument for problem solving through simulations of scientific and mathematics phenomenon and experiments; as well as measuring and monitoring scientific laboratory experiments. This paper will highlight on the design and development of the virtual Visualisation Laboratory for Science & Mathematics Content (VLab-SMC) based on the Cognitivist- Constructivist-Contextual development life cycle model as well as the Instructional Design (ID) model, in order to achieve its objectives in teaching and learning. However, this paper with only highlight one of the virtual labs within VLab-SMC that is, the Virtual Lab for teaching Chemistry (VLab- Chem). The development life cycle involves the educational media to be used, measurement of content, and the authoring and programming involved; whilst the ID model involves the application of the cognitivist, constructivist and contextual theories in the modeling of the modules of VLab-SMC generally and Vlab-Chem specifically, using concepts such as 'learning by doing', contextual learning, experimental simulations 3D and real

Outlet Glacier-Ice Shelf-Ocean Interactions: Is the Tail Wagging the Dog?

NASA Astrophysics Data System (ADS)

Parizek, B. R.; Walker, R. T.; Rinehart, S. K.

2009-12-01

While the massive interior regions of the Antarctic and Greenland Ice Sheets are presently ``resting quietly", the lower elevations of many outlet glaciers are experiencing dramatic adjustments due to changes in ice dynamics and/or surface mass balance. Oceanic and/or atmospheric forcing in these marginal regions often leads to mass deficits for entire outlet basins. Therefore, coupling the wagging tail of ice-ocean interactions with the vast ice-sheet reservoirs is imperative for accurate assessments of future sea-level rise. To study ice-ocean dynamic processes, we couple an ocean-plume model that simulates ice-shelf basal melting rates based on temperature and salinity profiles combined with plume dynamics associated with the geometry of the ice-shelf cavity (following Jenkins, 1991 and Holland and Jenkins, 1999) with a two-dimensional, isothermal model of outlet glacier-ice shelf flow (as used in Alley et al., 2007; Walker et al., 2008; Parizek et al., in review). Depending on the assigned temperature and salinity profiles, the ocean model can simulate both water-mass end-members: either cold High Salinity Shelf Water (HSSW) or relatively warm Circumpolar Deep Water (CDW), as well as between-member conditions. Notably, the coupled system exhibits sensitivity to the initial conditions. In particular, melting concentrated near the grounding line has the greatest effect in forcing grounding-line retreat. Retreat is further enhanced by a positive feedback between the ocean and ice, as the focused melt near the grounding line leads to an increase in the local slope of the basal ice, thereby enhancing buoyancy-driven plume flow and subsequent melt rates.

A critical developmental role for tgfbr2 in myogenic cell lineages is revealed in mice expressing SM22-Cre, not SMMHC-Cre.

PubMed

Frutkin, Andrew D; Shi, Haikun; Otsuka, Goro; Levéen, Per; Karlsson, Stefan; Dichek, David A

2006-10-01

Smooth muscle cell (SMC)-specific deletion of transforming growth factor beta (TGF-beta) signaling would help elucidate the mechanisms through which TGF-beta signaling contributes to vascular development and disease. We attempted to generate mice with SMC-specific deletion of TGF-beta signaling by mating mice with a conditional ("floxed") allele for the type II TGF-beta receptor (tgfbr2flox) to mice with SMC-targeted expression of Cre recombinase. We bred male mice transgenic for smooth muscle myosin heavy chain (SMMHC)-Cre with females carrying tgfbr2flox. Surprisingly, SMMHC-Cre mice recombined tgfbr2flox at low levels in SMC and at high levels in the testis. Recombination of tgfbr2flox in testis correlated with high-level expression of SMMHC-Cre in testis and germline transmission of tgfbr2null. In contrast, mice expressing Cre from a SM22alpha promoter (SM22-Cre) efficiently recombined tgfbr2flox in vascular and visceral SMC and the heart, but not in testis. Use of the R26R reporter allele confirmed that Cre-mediated recombination in vascular SMC was inefficient for SMMHC-Cre mice and highly efficient for SM22-Cre mice. Breedings that introduced the SM22-Cre allele into tgfbr2flox/flox zygotes in order to generate adult mice that are hemizygous for SM22-Cre and homozygous for tgfbr2flox- and would have conversion of tgfbr2flox/flox to tgfbr2null/null in SMC-produced no live SM22-Cre : tgfbr2flox/flox pups (P<0.001). We conclude: (1) "SMC-targeted" Cre lines vary significantly in specificity and efficiency of Cre expression; (2) TGF-beta signaling in the subset of cells that express SM22alpha is required for normal development; (3) generation of adult mice with absent TGF-beta signaling in SMC remains a challenge.

46 CFR 54.05-3 - Tests required.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Tests required. 54.05-3 Section 54.05-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Toughness Tests § 54.05-3 Tests required. (a) Where material or welding toughness tests are required by §§ 54.25-10, 54...

Science and Technology (S and T) Roadmap Collaboration between SMC, NASA, and Government Partners

NASA Technical Reports Server (NTRS)

Betser, Joseph; Ewart, Roberta; Chandler, Faith

2016-01-01

National Security Space (NSS) presents multi-faceted S and T challenges. We must continually innovate enterprise and information management; provide decision support; develop advanced materials; enhance sensor technology; transform communication technology; develop advanced propulsion and resilient space architectures and capabilities; and enhance multiple additional S and T domains. These challenges are best met by leveraging advanced S and T research and technology development from a number of DoD agencies and civil agencies such as NASA. The authors of this paper have engaged in these activities since 2006 and over the past decade developed multiple strategic S and T relationships. This paper highlights the Office of the Space Missile Systems Center (SMC) Chief Scientist (SMC/ST) collaboration with the NASA Office of Chief Technologist (NASA OCT), which has multiple S and T activities that are relevant to NSS. In particular we discuss the development of the Technology Roadmaps that benefit both Civil Space and NSS. Our collaboration with NASA OCT has been of mutual benefit to multiple participants. Some of the other DoD components include the Defense Advanced Research Projects agency (DARPA), Air Force Research Laboratory (AFRL), Naval Research Laboratory (NRL), The USAF Office of Chief Scientist, the USAF Science Advisory Board (SAB), Space and Naval Warfare Systems Command (SPAWAR), and a number of other services and agencies. In addition, the human talent is a key enabler of advanced S and T activities; it is absolutely critical to have a strong supply of talent in the fields of Science Technology, Engineering, and Mathematics (STEM). Consequently, we continually collaborate with the USAF Institute of Technology (AFIT), other service academies and graduate schools, and other universities and colleges. This paper highlights the benefits that result from such strategic S and T partnerships and recommends a way forward that will continually build upon these

10 CFR 54.7 - Written communications.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Written communications. 54.7 Section 54.7 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) REQUIREMENTS FOR RENEWAL OF OPERATING LICENSES FOR NUCLEAR POWER PLANTS General Provisions § 54.7 Written communications. All applications, correspondence, reports, and other...

10 CFR 54.7 - Written communications.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 2 2011-01-01 2011-01-01 false Written communications. 54.7 Section 54.7 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) REQUIREMENTS FOR RENEWAL OF OPERATING LICENSES FOR NUCLEAR POWER PLANTS General Provisions § 54.7 Written communications. All applications, correspondence, reports, and other...

Discovery of radio emission from the symbiotic X-ray binary system GX 1+4

NASA Astrophysics Data System (ADS)

van den Eijnden, J.; Degenaar, N.; Russell, T. D.; Miller-Jones, J. C. A.; Wijnands, R.; Miller, J. M.; King, A. L.; Rupen, M. P.

2018-02-01

We report the discovery of radio emission from the accreting X-ray pulsar and symbiotic X-ray binary GX 1+4 with the Karl G. Jansky Very Large Array. This is the first radio detection of such a system, wherein a strongly magnetized neutron star accretes from the stellar wind of an M-type giant companion. We measure a 9 GHz radio flux density of 105.3 ± 7.3 μJy, but cannot place meaningful constraints on the spectral index due to a limited frequency range. We consider several emission mechanisms that could be responsible for the observed radio source. We conclude that the observed properties are consistent with shocks in the interaction of the accretion flow with the magnetosphere, a synchrotron-emitting jet, or a propeller-driven outflow. The stellar wind from the companion is unlikely to be the origin of the radio emission. If the detected radio emission originates from a jet, it would show that strong magnetic fields (≥1012 G) do not necessarily suppress jet formation.

Rat Muc4 (sialomucin complex) reduces binding of anti-ErbB2 antibodies to tumor cell surfaces, a potential mechanism for herceptin resistance.

PubMed

Price-Schiavi, Shari A; Jepson, Scott; Li, Peter; Arango, Maria; Rudland, Philip S; Yee, Lisa; Carraway, Kermit L

2002-06-20

Muc4 (also called sialomucin complex), the rat homolog of human MUC4, is a heterodimeric glycoprotein complex that consists of a peripheral O-glycosylated mucin subunit, ASGP-1, tightly but noncovalently linked to a N-glycosylated transmembrane subunit, ASGP-2. The complex is expressed in a number of normal, vulnerable epithelial tissues, including mammary gland, uterus, colon, cornea and trachea. Muc4/SMC is also overexpressed or aberrantly expressed on a number of human tumors including breast tumors. Overexpression of Muc4/SMC has been shown to block cell-cell and cell-matrix interactions, protect tumor cells from immune surveillance and promote metastasis. In addition, as a ligand for ErbB2, Muc4/SMC can potentiate phosphorylation of ErbB2 and potentially alter signals generated from this receptor. Using A375 human melanoma cells and MCF7 human breast adenocarcinoma cells stably transfected with tetracycline regulatable Muc4, we have investigated whether overexpression of Muc4/SMC can repress antibody binding to cell surface-expressed ErbB2. Overexpression of Muc4/SMC does not affect the level of ErbB2 expression in either cell line, but it does reduce binding of a number of anti-ErbB2 antibodies, including Herceptin. Interestingly, overexpression of ErbB2 does not block binding of other unrelated antibodies of the same isotype, suggesting that the reduction in ErbB2 antibody binding is due to complex formation of Muc4/SMC and ErbB2. Furthermore, capping of Muc4/SMC with anti-Muc4/SMC antibodies reduces antibody binding to ErbB2 instead of increasing binding, again suggesting that reduced antibody binding to ErbB2 is due to steric hindrance from complex formation of Muc4/SMC and ErbB2. Thus, overexpression of Muc4/SMC on tumor cells may have both prognostic and therapeutic relevance. Copyright 2002 Wiley-Liss, Inc.

28 CFR 54.410 - Comparable facilities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Comparable facilities. 54.410 Section 54... in Education Programs or Activities Prohibited § 54.410 Comparable facilities. A recipient may provide separate toilet, locker room, and shower facilities on the basis of sex, but such facilities...

Structural and vibrational study of a neurotransmitter molecule: Dopamine [4-(2-aminoethyl) benzene-1,2-diol

NASA Astrophysics Data System (ADS)

Jha, Omkant; Yadav, T. K.; Yadav, R. A.

2018-01-01

Structural and vibrational studies for the most stable conformer of dopamine {4-(2-Aminoethyl) benzene-1, 2-diol} have been carried out at the DFT/B3LYP/6-311 ++G** level using the Gaussian 09 software. The IR and Raman spectra have been recorded and analyzed in light of the computed vibrational parameters using the DFT and the PEDs computed with the help of the GAR2PED software. Some of the fundamentals have considerably changed frequencies in going from benzene to dopamine. Except the rocking and wagging modes of the NH2 group the other four modes are pure group modes. The rocking and wagging modes of the NH2 group show mixing with the other modes. The two Osbnd H stretching vibrations are highly localized modes. The Kekule phenyl ring stretching mode is found to remain almost unchanged. The HOMO-LUMO study suggests the existence of charge transfer within the molecule and the energy gap supports the pharmacological active property of the dopamine molecule. The NBO analysis has been carried out to understand the proper and improper hydrogen bonding.

28 CFR 54.525 - Fringe benefits.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Fringe benefits. 54.525 Section 54.525... in Employment in Education Programs or Activities Prohibited § 54.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical, hospital...

28 CFR 54.525 - Fringe benefits.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Fringe benefits. 54.525 Section 54.525... in Employment in Education Programs or Activities Prohibited § 54.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical, hospital...

28 CFR 54.525 - Fringe benefits.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Fringe benefits. 54.525 Section 54.525... in Employment in Education Programs or Activities Prohibited § 54.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical, hospital...

28 CFR 54.525 - Fringe benefits.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Fringe benefits. 54.525 Section 54.525... in Employment in Education Programs or Activities Prohibited § 54.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical, hospital...

10 CFR 54.15 - Specific exemptions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Specific exemptions. 54.15 Section 54.15 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) REQUIREMENTS FOR RENEWAL OF OPERATING LICENSES FOR NUCLEAR POWER PLANTS General Provisions § 54.15 Specific exemptions. Exemptions from the requirements of this part may be...

Steel skin - SMC laminate structures for lightweight automotive manufacturing

NASA Astrophysics Data System (ADS)

Quagliato, Luca; Jang, Changsoon; Murugesan, Mohanraj; Kim, Naksoo

2017-09-01

In the present research work an innovative material, made of steel skin and sheet molding compound core, is presented and is aimed to be utilized for the production of automotive body frames. For a precise description of the laminate structure, the material properties of all the components, including the adhesive utilized as an interlayer, have been carried out, along with the simple tension test of the composite material. The result have shown that the proposed laminate structure has a specific yield strength 114% higher than 6061 T6 aluminum, 34% higher than 7075 T6 aluminum, 186% higher than AISI 304 stainless steel (30HRC) and 42% than SK5 high-strength steel (52HRC), showing its reliability and convenience for the realization of automotive components. After calibrating the material properties of the laminate structure, and utilizing as reference the simple tension results of the laminate structure, the derived material properties have been utilized for the simulation of the mechanical behavior of an automotive B-pillar. The results have been compared with those of a standard B-pillar made of steel, showing that the MS-SMC laminate structure manifests load and impact carry capacity comparable with those of high strength steel, while granting, at least, an 11% weight reduction.

46 CFR 54.30-1 - Scope.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Scope. 54.30-1 Section 54.30-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Mechanical Stress Relief § 54.30-1 Scope. (a) Certain pressure vessels may be mechanically stress relieved in accordance with the...

46 CFR 54.30-1 - Scope.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Scope. 54.30-1 Section 54.30-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Mechanical Stress Relief § 54.30-1 Scope. (a) Certain pressure vessels may be mechanically stress relieved in accordance with the...

46 CFR 54.30-1 - Scope.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Scope. 54.30-1 Section 54.30-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Mechanical Stress Relief § 54.30-1 Scope. (a) Certain pressure vessels may be mechanically stress relieved in accordance with the...

46 CFR 54.30-1 - Scope.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Scope. 54.30-1 Section 54.30-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Mechanical Stress Relief § 54.30-1 Scope. (a) Certain pressure vessels may be mechanically stress relieved in accordance with the...

46 CFR 54.30-1 - Scope.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Scope. 54.30-1 Section 54.30-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Mechanical Stress Relief § 54.30-1 Scope. (a) Certain pressure vessels may be mechanically stress relieved in accordance with the...

11 CFR 111.54 - Bankruptcy claims.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 11 Federal Elections 1 2012-01-01 2012-01-01 false Bankruptcy claims. 111.54 Section 111.54 Federal Elections FEDERAL ELECTION COMMISSION GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Collection of Debts Arising From Enforcement and Administration of Campaign Finance Laws § 111.54 Bankruptcy...

11 CFR 111.54 - Bankruptcy claims.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 11 Federal Elections 1 2011-01-01 2011-01-01 false Bankruptcy claims. 111.54 Section 111.54 Federal Elections FEDERAL ELECTION COMMISSION GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Collection of Debts Arising From Enforcement and Administration of Campaign Finance Laws § 111.54 Bankruptcy...

11 CFR 111.54 - Bankruptcy claims.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 11 Federal Elections 1 2014-01-01 2014-01-01 false Bankruptcy claims. 111.54 Section 111.54 Federal Elections FEDERAL ELECTION COMMISSION GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Collection of Debts Arising From Enforcement and Administration of Campaign Finance Laws § 111.54 Bankruptcy...

7 CFR 51.54 - Plant survey.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 2 2014-01-01 2014-01-01 false Plant survey. 51.54 Section 51.54 Agriculture... Contract Basis § 51.54 Plant survey. Prior to the inauguration of continuous Federal or Federal-State Inspection Service on a contract basis, the Administrator will make or cause to be made a survey and...

7 CFR 51.54 - Plant survey.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 2 2013-01-01 2013-01-01 false Plant survey. 51.54 Section 51.54 Agriculture... Contract Basis § 51.54 Plant survey. Prior to the inauguration of continuous Federal or Federal-State Inspection Service on a contract basis, the Administrator will make or cause to be made a survey and...

11 CFR 111.54 - Bankruptcy claims.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 11 Federal Elections 1 2013-01-01 2012-01-01 true Bankruptcy claims. 111.54 Section 111.54 Federal Elections FEDERAL ELECTION COMMISSION GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Collection of Debts Arising From Enforcement and Administration of Campaign Finance Laws § 111.54 Bankruptcy claims...

D- and L-[123I]-2-I-phenylalanine show a long tumour retention compared with D- and L-[123I]-2-I-tyrosine in R1M rhabdomyosarcoma tumour-bearing Wag/Rij rats.

PubMed

Bauwens, Matthias; Lahoutte, Tony; Kersemans, Ken; Caveliers, Vicky; Bossuyt, Axel; Mertens, John

2007-07-01

The aim of this study was the comparison of the tumour uptake and the long-term retention of [(123)I]-2-I-L-phenylalanine and [(123)I]-2-I-D-phenylalanine with those of [(123)I]-2-I-L-tyrosine and [(123)I]-2-I-D-tyrosine in R1M rhabdomyosarcoma tumour-bearing rats. The biodistribution of the radioactivity as a function of time in R1M tumour-bearing rats was measured by planar gamma camera imaging (dynamic and static). If dissection was applied, the activity in the tumours and tissues of interest was measured by gamma counting. [(123)I]-2-iodo-L-phenylalanine, [(123)I]-2-iodo-D-phenylalaine, [(123)I]-2-I-L-tyrosine showed a considerable tumour uptake reaching a maximum between 10 and 30 min. At 30 min p.i. the differential uptake ratio values of this uptake were, respectively, 2.1, 2.3, 2.5 and 1.7. The activity in the tumour was shown to be related to a tumour cell uptake and not to an increased blood pool activity. All the tracers showed a clearance from the blood to the bladder without renal retention. At longer times both L- and D- [(123)I]-2-I-tyrosine were cleared for a large part from the tumours and the body. [(123)I]-2-I-L-Phe and [(123)I]-2-I-D-Phe showed a considerable and equal retention in the tumours: as compared with 0.5 h, 91% at 24 h and 80% at 48 h. This was related to the longer retention of activity in the blood pool noticed for these compounds (81% at 24 h and 65% at 48 h). The tumour-to-background ratio increased with 25% at those longer times. At short times all the tracers were taken up to a considerable extent in the tumours. In the R1M-bearing Wag/Rij rat model only [(123)I]-2-I-L-phenylalanine and [(123)I]-2-I-D-phenylalanine showed an especially high retention at long times without any significant difference between the enantiomers. Copyright 2007 John Wiley & Sons, Ltd.

The HLA-B*54:35 and -B*54:38 identified in volunteer donors for hematopoietic stem cell transplant.

PubMed

Baek, I-C; Choi, E-J; Shin, D-H; Kim, H-J; Kim, T-G

2018-06-19

HLA-B*54:35 and -B*54:38 differ from HLA-B*54:01:01 in codons in exons 2 and 3 This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

7 CFR 51.54 - Plant survey.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 2 2012-01-01 2012-01-01 false Plant survey. 51.54 Section 51.54 Agriculture... Requirements for Plants Operating Under Continuous Inspection on A Contract Basis § 51.54 Plant survey. Prior..., the Administrator will make or cause to be made a survey and inspection where such service is to be...

7 CFR 51.54 - Plant survey.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 2 2010-01-01 2010-01-01 false Plant survey. 51.54 Section 51.54 Agriculture... Requirements for Plants Operating Under Continuous Inspection on A Contract Basis § 51.54 Plant survey. Prior..., the Administrator will make or cause to be made a survey and inspection where such service is to be...

7 CFR 51.54 - Plant survey.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 2 2011-01-01 2011-01-01 false Plant survey. 51.54 Section 51.54 Agriculture... Requirements for Plants Operating Under Continuous Inspection on A Contract Basis § 51.54 Plant survey. Prior..., the Administrator will make or cause to be made a survey and inspection where such service is to be...

Isoflurane waste anesthetic gas concentrations associated with the open-drop method.

PubMed

Taylor, Douglas K; Mook, Deborah M

2009-01-01

The open-drop technique is used frequently for anesthetic delivery to small rodents. Operator exposure to waste anesthetic gas (WAG) is a potential occupational hazard if this method is used without WAG scavenging. This study was conducted to determine whether administration of isoflurane by the open-drop technique without exposure controls generates significant WAG concentrations. We placed 0.1, 0.2, or 0.3 ml of liquid isoflurane into screw-top 500 or 1000 ml glass jars. WAG concentration was measured at the opening of the container and 20 and 40 cm from the opening, a distance at which users likely would operate, at 1, 2, or 3 min WAG was measured by using a portable infrared gas analyzer. Mean WAG concentrations at the vessel opening were as high as 662 +/- 168 ppm with a 500 ml jar and 122 +/- 87 ppm with a 1000 ml jar. At operator levels, WAG concentrations were always at or near 0 ppm. For measurements made at the vessel opening, time was the only factor that significantly affected WAG concentration when using the 500 ml jar. Neither time nor liquid volume were significant factors when using 1000 ml jar. At all liquid volumes and time points, the WAG concentration associated with using the 500 ml container was marginally to significantly greater than that for the 1000 ml jar.

46 CFR 54.25-1 - Scope.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Scope. 54.25-1 Section 54.25-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-1 Scope. The carbon, alloy, and heat treated steels used in construction of pressure vessels and parts shal...

46 CFR 54.25-1 - Scope.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Scope. 54.25-1 Section 54.25-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-1 Scope. The carbon, alloy, and heat treated steels used in construction of pressure vessels and parts shal...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.25-1 - Scope.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Scope. 54.25-1 Section 54.25-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-1 Scope. The carbon, alloy, and heat treated steels used in construction of pressure vessels and parts shal...

46 CFR 54.25-1 - Scope.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Scope. 54.25-1 Section 54.25-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-1 Scope. The carbon, alloy, and heat treated steels used in construction of pressure vessels and parts shal...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.03-1 - Scope.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Scope. 54.03-1 Section 54.03-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation § 54.03-1 Scope. The pressure vessels for low temperature operation shall be as required by section VIII of the ASME Boiler and Pressure Vessel...

46 CFR 54.25-1 - Scope.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Scope. 54.25-1 Section 54.25-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-1 Scope. The carbon, alloy, and heat treated steels used in construction of pressure vessels and parts shal...

28 CFR 54.500 - Employment.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Employment. 54.500 Section 54.500 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... employment on the basis of attendance at any educational institution or entity that admits as students only...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

46 CFR 54.01-2 - Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Adoption of division 1 of section VIII of the ASME Boiler and Pressure Vessel Code. 54.01-2 Section 54.01-2 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS General Requirements § 54.01-2 Adoption of division 1 of section VIII of the ASME Boiler and...

41 CFR 105-54.200 - Scope of subpart.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 41 Public Contracts and Property Management 3 2013-07-01 2013-07-01 false Scope of subpart. 105-54.200 Section 105-54.200 Public Contracts and Property Management Federal Property Management... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.200 Scope of...

41 CFR 105-54.200 - Scope of subpart.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Scope of subpart. 105-54.200 Section 105-54.200 Public Contracts and Property Management Federal Property Management... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.200 Scope of...

41 CFR 105-54.200 - Scope of subpart.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 41 Public Contracts and Property Management 3 2012-01-01 2012-01-01 false Scope of subpart. 105-54.200 Section 105-54.200 Public Contracts and Property Management Federal Property Management... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.200 Scope of...

41 CFR 105-54.200 - Scope of subpart.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 41 Public Contracts and Property Management 3 2011-01-01 2011-01-01 false Scope of subpart. 105-54.200 Section 105-54.200 Public Contracts and Property Management Federal Property Management... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.200 Scope of...

41 CFR 105-54.200 - Scope of subpart.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 41 Public Contracts and Property Management 3 2014-01-01 2014-01-01 false Scope of subpart. 105-54.200 Section 105-54.200 Public Contracts and Property Management Federal Property Management... Administration 54-ADVISORY COMMITTEE MANAGEMENT 54.2-Establishment of Advisory Committees § 105-54.200 Scope of...

Production and localization of Muc4/sialomucin complex and its receptor tyrosine kinase ErbB2 in the rat lacrimal gland.

PubMed

Arango, M E; Li, P; Komatsu, M; Montes, C; Carraway, C A; Carraway, K L

2001-11-01

To show the presence and forms of sialomucin complex (rat Muc4) and receptor tyrosine kinase ErbBs in the rat lacrimal gland and analyze for complexes of ErbB2 and its ligand Muc4. Northern blot analyses were used to identify sialomucin complex/Muc4 (SMC/Muc4) mRNA in rat lacrimal gland. Immunoblot analyses were performed to detect SMC/Muc4 and ErbBs. Sequential immunoprecipitation and immunoblot analyses were used to differentiate membrane and soluble forms of the SMC/Muc4 transmembrane subunit ASGP-2. Methacarn-fixed, paraffin-embedded sections of lacrimal glands from female adult rats were immunocytochemically stained using antisera to SMC/Muc4 and ErbBs to determine their relative locations in the gland. Colocalization of SMC/Muc4 and ErbB2 was confirmed by confocal immunofluorescence. Sequential immunoprecipitation and immunoblot were performed to analyze complexes of the SMC/Muc4 and ErbB2 in the lacrimal tissue. Northern blot analyses of rat lacrimal glands, with a probe for SMC/Muc4, demonstrated the presence of a approximately 9-kb transcript, consistent with observations in other tissues. Similarly, immunoblot analyses with antibodies against both the transmembrane (ASGP-2) and mucin (ASGP-1) subunits showed the presence of the two SMC/Muc4 subunits in lysates from rat lacrimal gland. Significantly, two different forms of ASGP-2 were observed, a high-molecular-weight ( approximately 200-kDa) form and the more common 120- to 140-kDa form. Sequential immunoprecipitation and immunoblot analyses to differentiate membrane and soluble forms of SMC/Muc4 indicated that the high-molecular-weight form of ASGP-2 was predominantly associated with membranes, whereas the 120- to 140-kDa form was both membrane-associated and soluble. The lacrimal gland consists of acini connected by intercalated and interlobular ducts. Both acini and some intercalated ducts were stained by anti-ASGP-2 monoclonal antisera. Two patterns of acinar staining were observed: membrane staining

[Effect of FABP2 gene G54A polymorphism on lipid and glucose metabolism in simple obesity children].

PubMed

Xu, Yunpeng; Rao, Xiaojiao; Hao, Min; Hou, Lijuan; Zhu, Xiaobo; Chang, Xiaotong

2016-01-01

To explore the relationship between intestinal fatty acid binding protein (FABP2) gene G54A polymorphism and simple childhood obesity, the effect of mutant 54A FABP2 gene on serum lipids and glucose metabolism. The total of 83 subjects with overweight/obesity and 100 subjects with healthy/normal weight were involved in this study. The G54A FABP2 gene allele and genotype frequencies between control group and overweight/obesity group were detected using polymerase chain reaction (PCR) -restriction fragment length polymorphism (RFLP) technology, and DNA sequences were confirmed by DNA sequencing. The automatic biochemical analyzer was used to detect fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels. Plasma insulin (Ins) was detected by radiation immune method, free fatty acids (FFA) was tested by ELISA method, insulin resistance index ( HOMA-IR ) was also calculated. The correlation between FABP2 G54A polymorphism and the development of children' obesity was analyzed. The relation between FABP2 G54A polymorphism and abnormal blood lipid and insulin resistance was assessed. The results of study on FABP2 gene polymorphism revealed as followed. In overweight/obese groups, the frequencies of GG, GA, AA genotypes was 33.7%, 49.4% and 16.9%, respectively. In control group, the frequencies of GG, GA, AA genotypes was 51. 0% , 40. 0% and 9. 0% , respectively. The differences between two groups was statistically significant (Χ2 = 6.27, P < 0.05). In overweight/obesity group, the frequencies of alleles were 58.4% for 54G and 41.6% for 54A. In control group, the frequencies of alleles were 71.0% for 54G and 29.0% for 54A. There was significant differences (Χ2 = 6.32, P < 0.05). The plasma biochemical variables results showed that compared with the normal control group, plasma TG (P < 0.01), Ins (P < 0.05), HOMA-IR (P < 0.05) were elevated in overweight

High-resolution study of Gamow-Teller transitions via the 54Fe(3He,t)54Co reaction

NASA Astrophysics Data System (ADS)

Adachi, T.; Fujita, Y.; Bacher, A. D.; Berg, G. P. A.; Black, T.; de Frenne, D.; Foster, C. C.; Fujita, H.; Fujita, K.; Hatanaka, K.; Honma, M.; Jacobs, E.; Jänecke, J.; Kanzaki, K.; Katori, K.; Nakanishi, K.; Negret, A.; Otsuka, T.; Popescu, L.; Roberts, D. A.; Sakemi, Y.; Shimbara, Y.; Shimizu, Y.; Stephenson, E. J.; Tameshige, Y.; Tamii, A.; Uchida, M.; Ueno, H.; Yamanaka, T.; Yosoi, M.; Zell, K. O.

2012-02-01

The Gamow-Teller transition strengths, B(GT), in pf-shell nuclei are of interest in nuclear physics as well as in nuclear astrophysics. A high-resolution (3He,t) charge-exchange (CE) reaction was performed on the Tz=+1 nucleus 54Fe at 0∘ and at an intermediate incident energy of 140 MeV/nucleon for the study of precise GT transition strengths to the final Tz=0 nucleus 54Co. By applying dispersion matching techniques for a high-quality 3He beam at RCNP, an energy resolution of 21 keV and an angular resolution of 5 mr were realized. The bumplike structure of the GT resonance observed in low-resolution CE reactions at around the excitation energy (Ex) of 10 MeV was resolved in individual L = 0, GT states. Excitation strengths were obtained for these GT states. If the R2 value that is defined by the ratio between GT and Fermi unit cross sections is known, the B(GT) values can be determined from the excitation strengths. For the derivation of the R2 value, the “merged analysis” combining the GT strength distribution from the 54Fe(3He,t)54Co study and the half-life from a 54Ni β decay was used, where T=1 isospin symmetry for A=54 isobars was assumed. The GT strengths were compared with a shell-model calculation using the GXPF1 interaction. The final GT states can have the isospin values T = 0, 1, and 2. The isospin T of each GT state observed in the 8.3≤Ex≤12.0 MeV region of the 54Fe(3He,t)54Co spectrum was identified by comparing the excitation strength with that of corresponding M1 state observed in a 54Fe(p,p')54Fe experiment. The B(GT) values of the states identified to have T=2, in particular, are of importance for the calculation of the electron capture rates at the core-collapse stage of presupernovae. The B(GT) strengths were further compared with B(M1) strengths measured in the 54Fe(e,e')54Fe reaction. In the M1 excitation using an electromagnetic probe, isoscalar (IS) and isovector (IV) orbital type operators are active in addition to the IV spin

Changes in corticocortical and corticohippocampal network during absence seizures in WAG/Rij rats revealed with time varying Granger causality.

PubMed

Sysoeva, Marina V; Vinogradova, Lyudmila V; Kuznetsova, Galina D; Sysoev, Ilya V; van Rijn, Clementina M

2016-11-01

Spike-and-wave discharges (SWDs) recorded in the cortical EEGs of WAG/Rij rats are the hallmark for absence epilepsy in this model. Although this type of epilepsy was long regarded as a form of primary generalized epilepsy, it is now recognized that there is an initiation zone - the perioral region of the somatosensory cortex. However, networks involved in spreading the seizure are not yet fully known. Previously, the dynamics of coupling between different layers of the perioral cortical region and between these zones and different thalamic nuclei was studied in time windows around the SWDs, using nonlinear Granger causality. The aim of the present study was to investigate, using the same method, the coupling dynamics between different regions of the cortex and between these regions and the hippocampus. Local field potentials were recorded in the frontal, parietal, and occipital cortices and in the hippocampus of 19 WAG/Rij rats. To detect changes in coupling reliably in a short time window, in order to provide a good temporal resolution, the innovative adapted time varying nonlinear Granger causality method was used. Mutual information function was calculated in addition to validate outcomes. Results of both approaches were tested for significance. The SWD initiation process was revealed as an increase in intracortical interactions starting from 3.5s before the onset of electrographic seizure. The earliest preictal increase in coupling was directed from the frontal cortex to the parietal cortex. Then, the coupling became bidirectional, followed by the involvement of the occipital cortex (1.5s before SWD onset). There was no driving from any cortical region to hippocampus, but a slight increase in coupling from hippocampus to the frontoparietal cortex was observed just before SWD onset. After SWD onset, an abrupt drop in coupling in all studied pairs was observed. In most of the pairs, the decoupling rapidly disappeared, but driving force from hippocampus and

Coating effect of LiFePO4 and Al2O3 on Li1.2Mn0.54Ni0.13Co0.13O2 cathode surface for lithium ion batteries

NASA Astrophysics Data System (ADS)

Seteni, Bonani; Rapulenyane, Nomasonto; Ngila, Jane Catherine; Mpelane, Siyasanga; Luo, Hongze

2017-06-01

Lithium-manganese-rich cathode material Li1.2Mn0.54Ni0.13Co0.13O2 is prepared by combustion method, and then coated with nano-sized LiFePO4 and nano-sized Al2O3 particles via a wet chemical process. The as-prepared Li1.2Mn0.54Ni0.13Co0.13O2, LiFePO4-coated Li1.2Mn0.54Ni0.13Co0.13O2 and Al2O3-coated Li1.2Mn0.54Ni0.13Co0.13O2 are characterized by X-ray diffraction, scanning electron microscopy and transmission electron microscopy. The scanning electron microscopy shows the agglomeration of the materials and their nanoparticle size ∼100 nm. The transmission electron microscopy confirms that LiFePO4 forms a rough mat-like surface and Al2O3 remain as islandic particles on the surface of the Li1.2Mn0.54Ni0.13Co0.13O2 material. The Li1.2Mn0.54Ni0.13Co0.13O2 coated with LiFePO4 and Li1.2Mn0.54Ni0.13Co0.13O2 coated with Al2O3 exhibits improved electrochemical performance. The initial discharge capacity is enhanced to 267 mAhg-1 after the LiFePO4 coating and 285 mAhg-1 after the Al2O3 coating compared to the as-prepared Li1.2Mn0.54Ni0.13Co0.13O2 material that has an initial discharge capacity of 243 mAhg-1. Galvanostatic charge-discharge tests at C/10 display longer activation of Li2MnO3 phase and higher capacity retention of 88% after 20 cycles for Li1.2Mn0.54Ni0.13Co0.13O2-LiFePO4 compared to Li1.2Mn0.54Ni0.13Co0.13O2-Al2O3 of 80% after 20 cycles and LMNC of 80% after 20 cycles. Meanwhile Li1.2Mn0.54Ni0.13Co0.13O2-LiFePO4 also shows higher rate capability compared to Li1.2Mn0.54Ni0.13Co0.13O2-Al2O3.

Accreting, highly magnetized neutron stars at the Eddington limit: a study of the 2016 outburst of SMC X-3

NASA Astrophysics Data System (ADS)

Koliopanos, Filippos; Vasilopoulos, Georgios

2018-06-01

Aims: We study the temporal and spectral characteristics of SMC X-3 during its recent (2016) outburst to probe accretion onto highly magnetized neutron stars (NSs) at the Eddington limit. Methods: We obtained XMM-Newton observations of SMC X-3 and combined them with long-term observations by Swift. We performed a detailed analysis of the temporal and spectral behavior of the source, as well as its short- and long-term evolution. We have also constructed a simple toy-model (based on robust theoretical predictions) in order to gain insight into the complex emission pattern of SMC X-3. Results: We confirm the pulse period of the system that has been derived by previous works and note that the pulse has a complex three-peak shape. We find that the pulsed emission is dominated by hard photons, while at energies below 1 keV, the emission does not pulsate. We furthermore find that the shape of the pulse profile and the short- and long-term evolution of the source light-curve can be explained by invoking a combination of a "fan" and a "polar" beam. The results of our temporal study are supported by our spectroscopic analysis, which reveals a two-component emission, comprised of a hard power law and a soft thermal component. We find that the latter produces the bulk of the non-pulsating emission and is most likely the result of reprocessing the primary hard emission by optically thick material that partly obscures the central source. We also detect strong emission lines from highly ionized metals. The strength of the emission lines strongly depends on the phase. Conclusions: Our findings are in agreement with previous works. The energy and temporal evolution as well as the shape of the pulse profile and the long-term spectra evolution of the source are consistent with the expected emission pattern of the accretion column in the super-critical regime, while the large reprocessing region is consistent with the analysis of previously studied X-ray pulsars observed at high

The fate of NGC602, an intense region of star-formation in the Wing of the SMC

NASA Astrophysics Data System (ADS)

Sabbi, Elena

2017-08-01

This is a small 2 orbit proposal designed to measure the internal dynamics of NGC602, a small region of intense star formation in the Wing of the SMC, with a low gas and dust density that has been often considered an unfavorable place for star formation. Small regions of massive star formation are important to study for our understanding of the process of star and cluster formation, the ionization of the interstellar medium, and the injection of energy and momentum into their host galaxy. By combining our new observations with archival ACS/WFC data acquired in July 2004, we will be able to measure the relative proper motions of the NGC602 sub-structures better than 2.3 km/s and investigate the nature of the apparently isolated massive stars found around NGC602. This study will provide unique observational data to characterize the early phase of cluster evolution and test cluster formation theories. It will also address significant open issues in star formation, cluster dynamics and the origin of isolated supernovae and GRBs.

Kv2 Channel Regulation of Action Potential Repolarization and Firing Patterns in Superior Cervical Ganglion Neurons and Hippocampal CA1 Pyramidal Neurons

PubMed Central

Liu, Pin W.

2014-01-01

Kv2 family “delayed-rectifier” potassium channels are widely expressed in mammalian neurons. Kv2 channels activate relatively slowly and their contribution to action potential repolarization under physiological conditions has been unclear. We explored the function of Kv2 channels using a Kv2-selective blocker, Guangxitoxin-1E (GxTX-1E). Using acutely isolated neurons, mixed voltage-clamp and current-clamp experiments were done at 37°C to study the physiological kinetics of channel gating and action potentials. In both rat superior cervical ganglion (SCG) neurons and mouse hippocampal CA1 pyramidal neurons, 100 nm GxTX-1E produced near-saturating block of a component of current typically constituting ∼60–80% of the total delayed-rectifier current. GxTX-1E also reduced A-type potassium current (IA), but much more weakly. In SCG neurons, 100 nm GxTX-1E broadened spikes and voltage clamp experiments using action potential waveforms showed that Kv2 channels carry ∼55% of the total outward current during action potential repolarization despite activating relatively late in the spike. In CA1 neurons, 100 nm GxTX-1E broadened spikes evoked from −70 mV, but not −80 mV, likely reflecting a greater role of Kv2 when other potassium channels were partially inactivated at −70 mV. In both CA1 and SCG neurons, inhibition of Kv2 channels produced dramatic depolarization of interspike voltages during repetitive firing. In CA1 neurons and some SCG neurons, this was associated with increased initial firing frequency. In all neurons, inhibition of Kv2 channels depressed maintained firing because neurons entered depolarization block more readily. Therefore, Kv2 channels can either decrease or increase neuronal excitability depending on the time scale of excitation. PMID:24695716

Lipid droplets induced by secreted phospholipase A2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress.

PubMed

Jarc, Eva; Kump, Ana; Malavašič, Petra; Eichmann, Thomas O; Zimmermann, Robert; Petan, Toni

2018-03-01

Cancer cells driven by the Ras oncogene scavenge unsaturated fatty acids (FAs) from their environment to counter nutrient stress. The human group X secreted phospholipase A 2 (hGX sPLA 2 ) releases FAs from membrane phospholipids, stimulates lipid droplet (LD) biogenesis in Ras-driven triple-negative breast cancer (TNBC) cells and enables their survival during starvation. Here we examined the role of LDs, induced by hGX sPLA 2 and unsaturated FAs, in protection of TNBC cells against nutrient stress. We found that hGX sPLA 2 releases a mixture of unsaturated FAs, including ω-3 and ω-6 polyunsaturated FAs (PUFAs), from TNBC cells. Starvation-induced breakdown of LDs induced by low micromolar concentrations of unsaturated FAs, including PUFAs, was associated with protection from cell death. Interestingly, adipose triglyceride lipase (ATGL) contributed to LD breakdown during starvation, but it was not required for the pro-survival effects of hGX sPLA 2 and unsaturated FAs. High micromolar concentrations of PUFAs, but not OA, induced oxidative stress-dependent cell death in TNBC cells. Inhibition of triacylglycerol (TAG) synthesis suppressed LD biogenesis and potentiated PUFA-induced cell damage. On the contrary, stimulation of LD biogenesis by hGX sPLA 2 and suppression of LD breakdown by ATGL depletion reduced PUFA-induced oxidative stress and cell death. Finally, lipidomic analyses revealed that sequestration of PUFAs in LDs by sPLA 2 -induced TAG remodelling and retention of PUFAs in LDs by inhibition of ATGL-mediated TAG lipolysis protect from PUFA lipotoxicity. LDs are thus antioxidant and pro-survival organelles that guard TNBC cells against nutrient and lipotoxic stress and emerge as attractive targets for novel therapeutic interventions. Copyright © 2017 Elsevier B.V. All rights reserved.

Effect of Ala54Thr polymorphism of FABP2 on anthropometric and biochemical variables in response to a moderate-fat diet.

PubMed

Martinez-Lopez, Erika; Garcia-Garcia, Maritza R; Gonzalez-Avalos, Jorge M; Maldonado-Gonzalez, Montserrat; Ruiz-Madrigal, Bertha; Vizmanos, Barbara; Hernandez-Nazara, Zamira; Roman, Sonia; Panduro, Arturo

2013-01-01

To analyze the effect of the fatty acid-binding protein (FABP2) gene Ala54Thr polymorphism on anthropometric and biochemical variables in response to a moderate-fat diet in overweight or obese subjects. One hundred nine subjects with a body mass index ≥ 25 kg/m(2) were studied. Participants underwent a dietary intervention that consisted of 30% fat (saturated fat <7% of total calories), 15% protein, and 55% carbohydrates. The FABP2 genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism. Anthropometric and biochemical data were measured at baseline, 1 mo, and 2 mo of nutritional intervention. The mean age was 38.6 ± 11.3 y and the mean body mass index 32.7 ± 6.1 kg/m(2), with 20 men (18%) and 89 women (82%). Fifty-three patients (48.6%) had genotype Ala54Ala (wild-type group) and 56 patients had genotype Ala54Thr/Thr54Thr (51.4%, mutant group). At baseline, no significant difference was found between the FABP2 genotypes groups, except for the carbohydrate intake and resting metabolic rate, which were higher in the Ala54Thr/Thr54Thr group (P < 0.05). At 2 mo, participants had lost 6.8% of their initial weight. The Ala54Thr/Thr54Thr group compared with the Ala54Ala group showed significant decreases in the parameters of weight (-7.5 versus -4.2 kg), body mass index (-2.1 versus -1.2 kg/m(2)), waist circumference (-7.6 versus -5.2 cm), waist-to-hip ratio (-0.04 versus -0.02), and C-reactive protein (-1.4 versus -0.76 mg/L), respectively (P < 0.05). After the resting metabolic rate was adjusted, the decreases in waist circumference, waist-to-hip ratio, and C-reactive protein remained significant between the two groups. This study showed that the Thr54 allele carriers responded better to a moderate-fat diet. Copyright © 2013 Elsevier Inc. All rights reserved.

17 CFR 201.54 - Settlement.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Settlement. 201.54 Section 201... Regulations Pertaining to the Equal Access to Justice Act § 201.54 Settlement. The applicant and counsel for the Office or Division of the Commission may agree on a proposed settlement of the award before final...

28 CFR 115.54 - Third-party reporting.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Third-party reporting. 115.54 Section 115.54 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Adult Prisons and Jails Reporting § 115.54 Third-party reporting. The agency shall...

28 CFR 115.54 - Third-party reporting.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Third-party reporting. 115.54 Section 115.54 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Adult Prisons and Jails Reporting § 115.54 Third-party reporting. The agency shall...

28 CFR 115.54 - Third-party reporting.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Third-party reporting. 115.54 Section 115.54 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) PRISON RAPE ELIMINATION ACT NATIONAL STANDARDS Standards for Adult Prisons and Jails Reporting § 115.54 Third-party reporting. The agency shall...

18 CFR 701.54 - Interagency Liaison Committee.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 18 Conservation of Power and Water Resources 2 2013-04-01 2012-04-01 true Interagency Liaison Committee. 701.54 Section 701.54 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Headquarters Organization § 701.54 Interagency Liaison Committee. There is established within the...

18 CFR 701.54 - Interagency Liaison Committee.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false Interagency Liaison Committee. 701.54 Section 701.54 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Headquarters Organization § 701.54 Interagency Liaison Committee. There is established within the...

18 CFR 701.54 - Interagency Liaison Committee.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Interagency Liaison Committee. 701.54 Section 701.54 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Headquarters Organization § 701.54 Interagency Liaison Committee. There is established within the...

18 CFR 701.54 - Interagency Liaison Committee.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 18 Conservation of Power and Water Resources 2 2012-04-01 2012-04-01 false Interagency Liaison Committee. 701.54 Section 701.54 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Headquarters Organization § 701.54 Interagency Liaison Committee. There is established within the...

18 CFR 701.54 - Interagency Liaison Committee.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Interagency Liaison Committee. 701.54 Section 701.54 Conservation of Power and Water Resources WATER RESOURCES COUNCIL COUNCIL ORGANIZATION Headquarters Organization § 701.54 Interagency Liaison Committee. There is established within the...

Structural and vibrational study of a neurotransmitter molecule: Dopamine [4-(2-aminoethyl) benzene-1,2-diol].

PubMed

Jha, Omkant; Yadav, T K; Yadav, R A

2018-01-15

Structural and vibrational studies for the most stable conformer of dopamine {4-(2-Aminoethyl) benzene-1, 2-diol} have been carried out at the DFT/B3LYP/6-311++G** level using the Gaussian 09 software. The IR and Raman spectra have been recorded and analyzed in light of the computed vibrational parameters using the DFT and the PEDs computed with the help of the GAR2PED software. Some of the fundamentals have considerably changed frequencies in going from benzene to dopamine. Except the rocking and wagging modes of the NH 2 group the other four modes are pure group modes. The rocking and wagging modes of the NH 2 group show mixing with the other modes. The two OH stretching vibrations are highly localized modes. The Kekule phenyl ring stretching mode is found to remain almost unchanged. The HOMO-LUMO study suggests the existence of charge transfer within the molecule and the energy gap supports the pharmacological active property of the dopamine molecule. The NBO analysis has been carried out to understand the proper and improper hydrogen bonding. Copyright © 2017. Published by Elsevier B.V.

Swift Observations of SMC X-3 during Its 2016-2017 Super-Eddington Outburst

NASA Astrophysics Data System (ADS)

Weng, Shan-Shan; Ge, Ming-Yu; Zhao, Hai-Hui; Wang, Wei; Zhang, Shuang-Nan; Bian, Wei-Hao; Yuan, Qi-Rong

2017-07-01

The Be X-ray pulsar SMC X-3 underwent a giant outburst from 2016 August to 2017 March, which was monitored with the Swift satellite. During the outburst, its broadband flux increased dramatically, and the unabsorbed X-ray luminosity reached an extreme value of ˜ {10}39 erg s-1 around August 24. Using the Swift/XRT data, we measured the observed pulse frequency of the neutron star to compute the orbital parameters of the binary system. After applying the orbital corrections to Swift observations, we found that the spin frequency increased steadily from 128.02 mHz on August 10 and approached the spin equilibrium of ˜128.74 mHz in 2017 January with an unabsorbed luminosity of {L}{{X}}˜ 2× {10}37 erg s-1, indicating a strong dipolar magnetic field of B˜ 6.8× {10}12 G at the neutron star surface. The spin-up rate is tightly correlated with its X-ray luminosity during the super-Eddington outburst. The pulse profile in the Swift/XRT data is variable, showing double peaks at the early stage of outburst and then merging into a single peak at low luminosity. Additionally, we report that a low-temperature ({kT}˜ 0.2 keV) thermal component emerges in the phase-averaged spectra as the flux decays, and it may be produced from the outer truncated disk or the boundary layer between the exterior flow and the magnetosphere.

46 CFR 54.25-5 - Corrosion allowance.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Corrosion allowance. 54.25-5 Section 54.25-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-5 Corrosion allowance. The corrosion allowance...

46 CFR 54.25-5 - Corrosion allowance.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Corrosion allowance. 54.25-5 Section 54.25-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-5 Corrosion allowance. The corrosion allowance...

46 CFR 54.25-5 - Corrosion allowance.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Corrosion allowance. 54.25-5 Section 54.25-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-5 Corrosion allowance. The corrosion allowance...

46 CFR 54.25-5 - Corrosion allowance.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Corrosion allowance. 54.25-5 Section 54.25-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-5 Corrosion allowance. The corrosion allowance...

46 CFR 54.25-5 - Corrosion allowance.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Corrosion allowance. 54.25-5 Section 54.25-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Construction With Carbon, Alloy, and Heat Treated Steels § 54.25-5 Corrosion allowance. The corrosion allowance...

Inhibition of mutant BRAF splice variant signaling by next-generation, selective RAF inhibitors.

PubMed

Basile, Kevin J; Le, Kaitlyn; Hartsough, Edward J; Aplin, Andrew E

2014-05-01

Vemurafenib and dabrafenib block MEK-ERK1/2 signaling and cause tumor regression in the majority of advanced-stage BRAF(V600E) melanoma patients; however, acquired resistance and paradoxical signaling have driven efforts for more potent and selective RAF inhibitors. Next-generation RAF inhibitors, such as PLX7904 (PB04), effectively inhibit RAF signaling in BRAF(V600E) melanoma cells without paradoxical effects in wild-type cells. Furthermore, PLX7904 blocks the growth of vemurafenib-resistant BRAF(V600E) cells that express mutant NRAS. Acquired resistance to vemurafenib and dabrafenib is also frequently driven by expression of mutation BRAF splice variants; thus, we tested the effects of PLX7904 and its clinical analog, PLX8394 (PB03), in BRAF(V600E) splice variant-mediated vemurafenib-resistant cells. We show that paradox-breaker RAF inhibitors potently block MEK-ERK1/2 signaling, G1/S cell cycle events, survival and growth of vemurafenib/PLX4720-resistant cells harboring distinct BRAF(V600E) splice variants. These data support the further investigation of paradox-breaker RAF inhibitors as a second-line treatment option for patients failing on vemurafenib or dabrafenib. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Vibrational mode frequencies of H2S and H2O adsorbed on Ge(0 0 1)-(2 × 1) surfaces

NASA Astrophysics Data System (ADS)

Hartnett, M.; Fahy, S.

2015-02-01

The equilibrium geometry and vibrational modes of H2S and H2O-terminated Ge(0 0 1)-(2 × 1) surfaces are calculated in a supercell approach using first-principles density functional theory in the local density (LDA), generalized gradient (GGA) approximations and van der Waals (vdW) interactions. Mode frequencies are found using the frozen phonon method. For the H2S-passivated surface, the calculated frequencies in LDA (GGA) are 2429 cm-1 (2490) for the Hsbnd S stretch mode, 712 cm-1 (706) for the Hsbnd S bond bending mode, 377 cm-1 (36) for the Gesbnd S stretch mode and 328 cm-1 (337) for Hsbnd S wag mode. Frequencies for the H2O passivated surface are 3590 cm-1 (3600) for the Hsbnd O stretch mode, 921 cm-1 (947) for the bending mode, 609 cm-1 (559) for the Gesbnd O stretch, 1995 cm-1 (1991) for the Gesbnd H stretch mode, 498 cm-1 (478) for the Gesbnd H bending mode and 342 cm-1 (336) for the Hsbnd O wag mode. The differences between the functionals including vdW terms and the LDA or GGA are less than the differences between LDA and GGA for the vibrational mode frequencies.

A Review of NCI’s Extramural Grant Portfolio: Identifying Opportunities for Future Research in Genes and Environment in Cancer

PubMed Central

Ghazarian, Armen A.; Simonds, Naoko I.; Bennett, Kelly; Pimentel, Camilla B.; Ellison, Gary L.; Gillanders, Elizabeth M.; Schully, Sheri D.; Mechanic, Leah E.

2013-01-01

Background Genetic and environmental factors jointly influence cancer risk. The National Institutes of Health (NIH) has made the study of gene-environment (GxE) interactions a research priority since the year 2000. Methods To assess the current status of GxE research in cancer, we analyzed the extramural grant portfolio of the National Cancer Institute (NCI) from Fiscal Years 2007 to 2009. Publications attributed to selected grants were also evaluated. Results From the 1,106 research grants identified in our portfolio analysis, a random sample of 450 grants (40%) was selected for data abstraction; of these, 147 (33%) were considered relevant. The most common cancer type was breast (20%, n=29), followed by lymphoproliferative (10%, n=14), colorectal (9%, n=13), melanoma/other skin (9%, n=13), and lung/upper aero-digestive tract (8%, n=12) cancers. The majority of grants were studies of candidate genes (68%, n=100) compared to genome-wide association studies (GWAS) (8%, n=12). Approximately one third studied environmental exposures categorized as energy balance (37%, n=54) or drugs/treatment (29%, n=43). From the 147 relevant grants, 108 publications classified as GxE or pharmacogenomic were identified. These publications were linked to 37 of the 147 grant applications (25%). Conclusion The findings from our portfolio analysis suggest that GxE studies are concentrated in specific areas. There is room for investments in other aspects of GxE research, including, but not limited to developing alternative approaches to exposure assessment, broadening the spectrum of cancer types investigated, and performing GxE within GWAS. Impact This portfolio analysis provides a cross-sectional review of NCI support for GxE research in cancer. PMID:23462918

A review of NCI's extramural grant portfolio: identifying opportunities for future research in genes and environment in cancer.

PubMed

Ghazarian, Armen A; Simonds, Naoko I; Bennett, Kelly; Pimentel, Camilla B; Ellison, Gary L; Gillanders, Elizabeth M; Schully, Sheri D; Mechanic, Leah E

2013-04-01

Genetic and environmental factors jointly influence cancer risk. The NIH has made the study of gene-environment (GxE) interactions a research priority since the year 2000. To assess the current status of GxE research in cancer, we analyzed the extramural grant portfolio of the National Cancer Institute (NCI) from Fiscal Years 2007 to 2009. Publications attributed to selected grants were also evaluated. From the 1,106 research grants identified in our portfolio analysis, a random sample of 450 grants (40%) was selected for data abstraction; of these, 147 (33%) were considered relevant. The most common cancer type was breast (20%, n = 29), followed by lymphoproliferative (10%, n = 14), colorectal (9%, n = 13), melanoma/other skin (9%, n = 13), and lung/upper aerodigestive tract (8%, n = 12) cancers. The majority of grants were studies of candidate genes (68%, n = 100) compared with genome-wide association studies (GWAS) (8%, n = 12). Approximately one-third studied environmental exposures categorized as energy balance (37%, n = 54) or drugs/treatment (29%, n = 43). From the 147 relevant grants, 108 publications classified as GxE or pharmacogenomic were identified. These publications were linked to 37 of the 147 grant applications (25%). The findings from our portfolio analysis suggest that GxE studies are concentrated in specific areas. There is room for investments in other aspects of GxE research, including, but not limited to developing alternative approaches to exposure assessment, broadening the spectrum of cancer types investigated, and conducting GxE within GWAS. This portfolio analysis provides a cross-sectional review of NCI support for GxE research in cancer.

28 CFR 54.605 - Enforcement procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Enforcement procedures. 54.605 Section 54.605 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... procedures. The investigative, compliance, and enforcement procedural provisions of Title VI of the Civil...

28 CFR 54.605 - Enforcement procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Enforcement procedures. 54.605 Section 54.605 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... procedures. The investigative, compliance, and enforcement procedural provisions of Title VI of the Civil...

28 CFR 54.605 - Enforcement procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Enforcement procedures. 54.605 Section 54.605 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... procedures. The investigative, compliance, and enforcement procedural provisions of Title VI of the Civil...

28 CFR 54.605 - Enforcement procedures.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Enforcement procedures. 54.605 Section 54.605 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... procedures. The investigative, compliance, and enforcement procedural provisions of Title VI of the Civil...

28 CFR 54.605 - Enforcement procedures.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Enforcement procedures. 54.605 Section 54.605 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... procedures. The investigative, compliance, and enforcement procedural provisions of Title VI of the Civil...

46 CFR 54.03-5 - General.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false General. 54.03-5 Section 54.03-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation... minimum service temperature may be used in warmer service. Steels differing in chemical composition...

46 CFR 54.03-5 - General.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false General. 54.03-5 Section 54.03-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation... minimum service temperature may be used in warmer service. Steels differing in chemical composition...

46 CFR 54.03-5 - General.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false General. 54.03-5 Section 54.03-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation... minimum service temperature may be used in warmer service. Steels differing in chemical composition...

46 CFR 54.03-5 - General.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false General. 54.03-5 Section 54.03-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation... minimum service temperature may be used in warmer service. Steels differing in chemical composition...

46 CFR 54.03-5 - General.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false General. 54.03-5 Section 54.03-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Low Temperature Operation... minimum service temperature may be used in warmer service. Steels differing in chemical composition...

Operational space weather product development and validation at the joint SMC-AFRL Rapid Prototyping Center

NASA Astrophysics Data System (ADS)

Quigley, S.

The Air Force Research Laboratory (AFRL/VSB) and Detachment 11, Space &Missile Systems Center (SMC, Det 11/CIT) have combined efforts to design, develop, test, and implement graphical products for the Air Force's space weather operations center. These products are generated to analyze, specify, and forecast the effects of the near-earth space environment on Department of Defense systems and communications. Jointly-developed products that have been, or will soon be added to real-time operations include: 1) the Operational Space Environment Network Display (OpSEND) suit - a set of four products that address HF communication, UHF satellite communication scintillation, radar auroral clutter, and GP S single- frequency errors; 2) a solar radio background and burst effects (SoRBE) product suite; and C) a meteor effects (ME) product suite. The RPC is also involved in a rather substantial "V&V" effort to produce multiple operational product verifications and validations, with an added end goal of a generalized validation software package. The presentation will provide a general overview of the RPC and each of the products mentioned above, to include background science, operational history, inputs, outputs, dissemination, and customer uses for each.

28 CFR 54.130 - Effect of employment opportunities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Effect of employment opportunities. 54.130 Section 54.130 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE... § 54.130 Effect of employment opportunities. The obligation to comply with these Title IX regulations...

[Study on association of FABP2 gene Ala54Thr polymorphism with risk of obesity, body fat mass and physical activity].

PubMed

Nasibulina, É S; Borisova, A V; Akhmetov, I I

2013-01-01

Obesity is a multifactorial disease which depends on the interaction between genome and environment. Fatty acid-binding protein 2 (FABP2) regulates lipid transport, intestinal absorption and metabolism. The aim of the study was to investigate the interrelation between the FABP2 gene Ala54Thr polymorphism, body mass index and body fat mass and to study distribution of genotypes and alleles frequencies of FABP2 gene in athletes and individuals who are not involved in sports. 315 athletes of different sport disciplines and levels and 612 controls (predominantly students) participated in the study. Genotyping for the FABP2 gene Ala54Thr polymorphism was performed by PCR. Body composition was analyzed by bioimpedance method. The study did not confirm the association of FABP2 gene Ala54Thr polymorphism with the risk of obesity and body fat mass. However, the frequency of the Thr54 allele was significantly higher in elite stayers (50.0%, p = 0.025) and combat athletes (46.2%, p = 0.013) in comparison with controls (32.2%). Thus, FABP2 gene Ala54Thr polymorphism is associated with the predisposition to endurance athletic performance.

Runx2 Expression in Smooth Muscle Cells Is Required for Arterial Medial Calcification in Mice

PubMed Central

Lin, Mu-En; Chen, Theodore; Leaf, Elizabeth M.; Speer, Mei Y.; Giachelli, Cecilia M.

2016-01-01

Arterial medial calcification (AMC) is a hallmark of aging, diabetes, and chronic kidney disease. Smooth muscle cell (SMC) transition to an osteogenic phenotype is a common feature of AMC, and is preceded by expression of runt-related transcription factor 2 (Runx2), a master regulator of bone development. Whether SMC-specific Runx2 expression is required for osteogenic phenotype change and AMC remains unknown. We therefore created an improved targeting construct to generate mice with floxed Runx2 alleles (Runx2f/f) that do not produce truncated Runx2 proteins after Cre recombination, thereby preventing potential off-target effects. SMC-specific deletion using SM22–recombinase transgenic allele mice (Runx2ΔSM) led to viable mice with normal bone and arterial morphology. After vitamin D overload, arterial SMCs in Runx2f/f mice expressed Runx2, underwent osteogenic phenotype change, and developed severe AMC. In contrast, vitamin D–treated Runx2ΔSM mice had no Runx2 in blood vessels, maintained SMC phenotype, and did not develop AMC. Runx2 deletion did not affect serum calcium, phosphate, fibroblast growth factor-23, or alkaline phosphatase levels. In vitro, Runx2f/f SMCs calcified to a much greater extent than those derived from Runx2ΔSM mice. These data indicate a critical role of Runx2 in SMC osteogenic phenotype change and mineral deposition in a mouse model of AMC, suggesting that Runx2 and downstream osteogenic pathways in SMCs may be useful therapeutic targets for treating or preventing AMC in high-risk patients. PMID:25987250

28 CFR 550.54 - Incentives for RDAP participation.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Incentives for RDAP participation. 550.54 Section 550.54 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT DRUG PROGRAMS Drug Abuse Treatment Program § 550.54 Incentives for RDAP participation. (a) An inmate...

28 CFR 550.54 - Incentives for RDAP participation.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Incentives for RDAP participation. 550.54 Section 550.54 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT DRUG PROGRAMS Drug Abuse Treatment Program § 550.54 Incentives for RDAP participation. (a) An inmate...

28 CFR 550.54 - Incentives for RDAP participation.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 28 Judicial Administration 2 2012-07-01 2012-07-01 false Incentives for RDAP participation. 550.54 Section 550.54 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT DRUG PROGRAMS Drug Abuse Treatment Program § 550.54 Incentives for RDAP participation. (a) An inmate...

28 CFR 550.54 - Incentives for RDAP participation.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 2 2011-07-01 2011-07-01 false Incentives for RDAP participation. 550.54 Section 550.54 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT DRUG PROGRAMS Drug Abuse Treatment Program § 550.54 Incentives for RDAP participation. (a) An inmate...

28 CFR 550.54 - Incentives for RDAP participation.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Incentives for RDAP participation. 550.54 Section 550.54 Judicial Administration BUREAU OF PRISONS, DEPARTMENT OF JUSTICE INSTITUTIONAL MANAGEMENT DRUG PROGRAMS Drug Abuse Treatment Program § 550.54 Incentives for RDAP participation. (a) An inmate...

7 CFR 54.2 - Designation of official certificates, memoranda, marks, other identifications, and devices for...

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG PRODUCTS INSPECTION ACT (CONTINUED) MEATS, PREPARED MEATS, AND MEAT PRODUCTS (GRADING, CERTIFICATION, AND STANDARDS) Regulations Definitions § 54.2 Designation of...

7 CFR 54.2 - Designation of official certificates, memoranda, marks, other identifications, and devices for...

Code of Federal Regulations, 2011 CFR

2011-01-01

... AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG PRODUCTS INSPECTION ACT (CONTINUED) MEATS, PREPARED MEATS, AND MEAT PRODUCTS (GRADING, CERTIFICATION, AND STANDARDS) Regulations Definitions § 54.2 Designation of...

46 CFR 54.15-13 - Rupture disks (modifies UG-127).

Code of Federal Regulations, 2011 CFR

2011-10-01

...: (1) Comply with the general provisions of § 54.15-5 except as noted otherwise in this section; (2... 46 Shipping 2 2011-10-01 2011-10-01 false Rupture disks (modifies UG-127). 54.15-13 Section 54.15... VESSELS Pressure-Relief Devices § 54.15-13 Rupture disks (modifies UG-127). (a) Paragraph UG-127 of...

46 CFR 54.15-13 - Rupture disks (modifies UG-127).

Code of Federal Regulations, 2010 CFR

2010-10-01

...: (1) Comply with the general provisions of § 54.15-5 except as noted otherwise in this section; (2... 46 Shipping 2 2010-10-01 2010-10-01 false Rupture disks (modifies UG-127). 54.15-13 Section 54.15... VESSELS Pressure-Relief Devices § 54.15-13 Rupture disks (modifies UG-127). (a) Paragraph UG-127 of...

46 CFR 54.15-13 - Rupture disks (modifies UG-127).

Code of Federal Regulations, 2013 CFR

2013-10-01

...: (1) Comply with the general provisions of § 54.15-5 except as noted otherwise in this section; (2... 46 Shipping 2 2013-10-01 2013-10-01 false Rupture disks (modifies UG-127). 54.15-13 Section 54.15... VESSELS Pressure-Relief Devices § 54.15-13 Rupture disks (modifies UG-127). (a) Paragraph UG-127 of...

46 CFR 54.15-13 - Rupture disks (modifies UG-127).

Code of Federal Regulations, 2014 CFR

2014-10-01

...: (1) Comply with the general provisions of § 54.15-5 except as noted otherwise in this section; (2... 46 Shipping 2 2014-10-01 2014-10-01 false Rupture disks (modifies UG-127). 54.15-13 Section 54.15... VESSELS Pressure-Relief Devices § 54.15-13 Rupture disks (modifies UG-127). (a) Paragraph UG-127 of...

46 CFR 54.15-13 - Rupture disks (modifies UG-127).

Code of Federal Regulations, 2012 CFR

2012-10-01

...: (1) Comply with the general provisions of § 54.15-5 except as noted otherwise in this section; (2... 46 Shipping 2 2012-10-01 2012-10-01 false Rupture disks (modifies UG-127). 54.15-13 Section 54.15... VESSELS Pressure-Relief Devices § 54.15-13 Rupture disks (modifies UG-127). (a) Paragraph UG-127 of...

A study of accretion discs around rapidly rotating neutron stars in general relativity and its applications to four low mass X-ray binaries

NASA Astrophysics Data System (ADS)

Bhattacharyya, Sudip

2002-02-01

We calculate the accretion disc temperature profiles, disc luminosities and boundary layer luminosities for rapidly rotating neutron stars considering the full effect of general relativity. We compare the theoretical values of these quantities with their values inferred from EXOSAT data for four low mass X-ray binary sources: XB 1820-30, GX 17+2, GX 9+1 and GX 349+2 and constrain the values of several properties of these sources. According to our calculations, the neutron stars in GX 9+1 and GX 349+2 are rapidly rotating and stiffer equations of state are unfavoured.

Spectro-Timing Study of GX 339-4 in a Hard Intermediate State

NASA Technical Reports Server (NTRS)

Furst, F.; Grinberg, V.; Tomsick, J. A.; Bachetti, M.; Boggs, S. E.; Brightman, M.; Christensen, F. E.; Craig, W. W.; Ghandi, P.; Zhang, William W.

2016-01-01

We present an analysis of Nuclear Spectroscopic Telescope Array observations of a hard intermediate state of the transient black hole GX 339-4 taken in 2015 January. With the source softening significantly over the course of the 1.3 day long observation we split the data into 21 sub-sets and find that the spectrum of all of them can be well described by a power-law continuum with an additional relativistically blurred reflection component. The photon index increases from approx. 1.69 to approx. 1.77 over the course of the observation. The accretion disk is truncated at around nine gravitational radii in all spectra. We also perform timing analysis on the same 21 individual data sets, and find a strong type-C quasi-periodic oscillation (QPO), which increases in frequency from approx. 0.68 to approx. 1.05 Hz with time. The frequency change is well correlated with the softening of the spectrum. We discuss possible scenarios for the production of the QPO and calculate predicted inner radii in the relativistic precession model as well as the global disk mode oscillations model. We find discrepancies with respect to the observed values in both models unless we allow for a black hole mass of approx. 100 Mass compared to the Sun, which is highly unlikely. We discuss possible systematic uncertainties, in particular with the measurement of the inner accretion disk radius in the relativistic reflection model. We conclude that the combination of observed QPO frequencies and inner accretion disk radii, as obtained from spectral fitting, is difficult to reconcile with current models.

Comparing biosimilar SB2 with reference infliximab after 54 weeks of a double-blind trial: clinical, structural and safety results.

PubMed

Smolen, Josef S; Choe, Jung-Yoon; Prodanovic, Nenad; Niebrzydowski, Jaroslaw; Staykov, Ivan; Dokoupilova, Eva; Baranauskaite, Asta; Yatsyshyn, Roman; Mekic, Mevludin; Porawska, Wieskawa; Ciferska, Hana; Jedrychowicz-Rosiak, Krystyna; Zielinska, Agnieszka; Choi, Jasmine; Rho, Young Hee

2017-10-01

SB2 is a biosimilar to the reference infliximab (INF). Similar efficacy, safety and immunogenicity between SB2 and INF up to 30 weeks were previously reported. This report investigates such clinical similarity up to 54 weeks, including structural joint damage. In this phase III, double-blind, parallel-group, multicentre study, patients with moderate to severe RA despite MTX were randomized (1:1) to receive 3 mg/kg of either SB2 or INF at 0, 2, 6 and every 8 weeks thereafter. Dose escalation by 1.5 mg/kg up to a maximum dose of 7.5 mg/kg was allowed after week 30. Efficacy, safety and immunogenicity were measured at each visit up to week 54. Radiographic damage evaluated by modified total Sharp score was measured at baseline and week 54. A total of 584 patients were randomized to receive SB2 (n = 291) or INF (n = 293). The rate of radiographic progression was comparable between SB2 and INF (mean modified total Sharp score difference: SB2, 0.38; INF, 0.37) at 1 year. ACR responses, 28-joint DAS, Clinical Disease Activity Index and Simplified Disease Activity Index were comparable between SB2 and INF up to week 54. The incidence of treatment-emergent adverse events and anti-drug antibodies were comparable between treatment groups. Such comparable trends of efficacy, safety and immunogenicity were consistent from baseline up to 54 weeks. The pattern of dose increment was also comparable between SB2 and INF. SB2 maintained similar efficacy, safety and immunogenicity with INF up to 54 weeks in patients with moderate to severe RA. Radiographic progression was comparable at 1 year. ClinicalTrials.gov (http://clinicaltrials.gov; NCT01936181) and EudraCT (https://www.clinicaltrialsregister.eu; 2012-005733-37). © The Author 2017. Published by Oxford University Press on behalf of the British Society for Rheumatology.

Comparing biosimilar SB2 with reference infliximab after 54 weeks of a double-blind trial: clinical, structural and safety results

PubMed Central

Smolen, Josef S.; Choe, Jung-Yoon; Prodanovic, Nenad; Niebrzydowski, Jaroslaw; Staykov, Ivan; Dokoupilova, Eva; Baranauskaite, Asta; Yatsyshyn, Roman; Mekic, Mevludin; Porawska, Wieskawa; Ciferska, Hana; Jedrychowicz-Rosiak, Krystyna; Zielinska, Agnieszka; Choi, Jasmine; Rho, Young Hee

2017-01-01

Abstract Objectives SB2 is a biosimilar to the reference infliximab (INF). Similar efficacy, safety and immunogenicity between SB2 and INF up to 30 weeks were previously reported. This report investigates such clinical similarity up to 54 weeks, including structural joint damage. Methods In this phase III, double-blind, parallel-group, multicentre study, patients with moderate to severe RA despite MTX were randomized (1:1) to receive 3 mg/kg of either SB2 or INF at 0, 2, 6 and every 8 weeks thereafter. Dose escalation by 1.5 mg/kg up to a maximum dose of 7.5 mg/kg was allowed after week 30. Efficacy, safety and immunogenicity were measured at each visit up to week 54. Radiographic damage evaluated by modified total Sharp score was measured at baseline and week 54. Results A total of 584 patients were randomized to receive SB2 (n = 291) or INF (n = 293). The rate of radiographic progression was comparable between SB2 and INF (mean modified total Sharp score difference: SB2, 0.38; INF, 0.37) at 1 year. ACR responses, 28-joint DAS, Clinical Disease Activity Index and Simplified Disease Activity Index were comparable between SB2 and INF up to week 54. The incidence of treatment-emergent adverse events and anti-drug antibodies were comparable between treatment groups. Such comparable trends of efficacy, safety and immunogenicity were consistent from baseline up to 54 weeks. The pattern of dose increment was also comparable between SB2 and INF. Conclusion SB2 maintained similar efficacy, safety and immunogenicity with INF up to 54 weeks in patients with moderate to severe RA. Radiographic progression was comparable at 1 year. Trial registration ClinicalTrials.gov (http://clinicaltrials.gov; NCT01936181) and EudraCT (https://www.clinicaltrialsregister.eu; 2012-005733-37) PMID:28957563

MEF2C-MYOCD and Leiomodin1 Suppression by miRNA-214 Promotes Smooth Muscle Cell Phenotype Switching in Pulmonary Arterial Hypertension

PubMed Central

Sahoo, Sanghamitra; Meijles, Daniel N.; Al Ghouleh, Imad; Tandon, Manuj; Cifuentes-Pagano, Eugenia; Sembrat, John; Rojas, Mauricio; Goncharova, Elena; Pagano, Patrick J.

2016-01-01

Background Vascular hyperproliferative disorders are characterized by excessive smooth muscle cell (SMC) proliferation leading to vessel remodeling and occlusion. In pulmonary arterial hypertension (PAH), SMC phenotype switching from a terminally differentiated contractile to synthetic state is gaining traction as our understanding of the disease progression improves. While maintenance of SMC contractile phenotype is reportedly orchestrated by a MEF2C-myocardin (MYOCD) interplay, little is known regarding molecular control at this nexus. Moreover, the burgeoning interest in microRNAs (miRs) provides the basis for exploring their modulation of MEF2C-MYOCD signaling, and in turn, a pro-proliferative, synthetic SMC phenotype. We hypothesized that suppression of SMC contractile phenotype in pulmonary hypertension is mediated by miR-214 via repression of the MEF2C-MYOCD-leiomodin1 (LMOD1) signaling axis. Methods and Results In SMCs isolated from a PAH patient cohort and commercially obtained hPASMCs exposed to hypoxia, miR-214 expression was monitored by qRT-PCR. miR-214 was upregulated in PAH- vs. control subject hPASMCs as well as in commercially obtained hPASMCs exposed to hypoxia. These increases in miR-214 were paralleled by MEF2C, MYOCD and SMC contractile protein downregulation. Of these, LMOD1 and MEF2C were directly targeted by the miR. Mir-214 overexpression mimicked the PAH profile, downregulating MEF2C and LMOD1. AntagomiR-214 abrogated hypoxia-induced suppression of the contractile phenotype and its attendant proliferation. Anti-miR-214 also restored PAH-PASMCs to a contractile phenotype seen during vascular homeostasis. Conclusions Our findings illustrate a key role for miR-214 in modulation of MEF2C-MYOCD-LMOD1 signaling and suggest that an antagonist of miR-214 could mitigate SMC phenotype changes and proliferation in vascular hyperproliferative disorders including PAH. PMID:27144530

Inhibition of metastin (kisspeptin-54)-GPR54 signaling in the arcuate nucleus-median eminence region during lactation in rats.

PubMed

Yamada, S; Uenoyama, Y; Kinoshita, M; Iwata, K; Takase, K; Matsui, H; Adachi, S; Inoue, K; Maeda, K-I; Tsukamura, H

2007-05-01

Follicular development and ovulation are suppressed during lactation in various mammalian species, mainly due to the suppression of pulsatile GnRH/LH secretion. Metastin (kisspeptin-54), a KiSS-1 gene product, is an endogenous ligand for GPR54, a G-protein-coupled receptor, and suggested to play a critical role in regulating the gonadal axis. The present study therefore aims to determine whether metastin (kisspeptin-54)-GPR54 signaling in discrete brain areas is inhibited by the suckling stimulus that causes suppression of LH secretion in lactating rats. Quantitative RT-PCR revealed that the KiSS-1 mRNA level was significantly lower in the arcuate nucleus (ARC)-median eminence region in lactating ovariectomized (OVX) and estrogen-treated OVX rats than in nonlactating controls. KiSS-1 mRNA in the anteroventral periventricular nucleus was kept at a low level in both lactating and nonlactating rats despite estrogen treatment. GPR54 mRNA levels were significantly lower in lactating than nonlactating rats in the anteroventral periventricular nucleus, but the levels in lactating mothers of the preoptic area and ARC-median eminence were comparable with nonlactating controls. Although KiSS-1 mRNA-expressing cells or metastin (kisspeptin-54) immunoreactivities were densely located in the ARC of nonlactating controls, few were found in the ARC of lactating OVX animals. Various doses of metastin (kisspeptin-54) (0.02, 0.2, and 2 nmol) injected into the third ventricle caused a significant increase in LH secretion in both lactating and nonlactating OVX rats, suggesting that lactating rats are responsive to metastin (kisspeptin-54) stimulus. Thus, the present study demonstrated that KiSS-1 mRNA/metastin (kisspeptin-54) expression is inhibited in the ARC by the suckling stimulus, suggesting that the inhibition is most probably involved in suppressing LH secretion in lactating rats.

46 CFR 54.05-16 - Production toughness testing.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Production toughness testing. 54.05-16 Section 54.05-16... Toughness Tests § 54.05-16 Production toughness testing. (a) For vessels of welded construction, production... welding procedures shall be the same as used in the fabrication of the vessel. From each test plate, one...

46 CFR 54.05-5 - Toughness test specimens.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Toughness test specimens. 54.05-5 Section 54.05-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Toughness Tests § 54.05-5 Toughness test specimens. (a) Charpy V-notch impact tests. Where required, Charpy V-notch tests shall be conducted in accordanc...

46 CFR 54.05-6 - Toughness test temperatures.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 2 2013-10-01 2013-10-01 false Toughness test temperatures. 54.05-6 Section 54.05-6... Toughness Tests § 54.05-6 Toughness test temperatures. Each toughness test must be conducted at temperatures not warmer than −20 °F or 10 °F below the minimum service temperature, whichever is lower, except that...

46 CFR 54.05-6 - Toughness test temperatures.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 2 2014-10-01 2014-10-01 false Toughness test temperatures. 54.05-6 Section 54.05-6... Toughness Tests § 54.05-6 Toughness test temperatures. Each toughness test must be conducted at temperatures not warmer than −20 °F or 10 °F below the minimum service temperature, whichever is lower, except that...

46 CFR 54.05-6 - Toughness test temperatures.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 2 2011-10-01 2011-10-01 false Toughness test temperatures. 54.05-6 Section 54.05-6... Toughness Tests § 54.05-6 Toughness test temperatures. Each toughness test must be conducted at temperatures not warmer than −20 °F or 10 °F below the minimum service temperature, whichever is lower, except that...

46 CFR 54.05-6 - Toughness test temperatures.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Toughness test temperatures. 54.05-6 Section 54.05-6... Toughness Tests § 54.05-6 Toughness test temperatures. Each toughness test must be conducted at temperatures not warmer than −20 °F or 10 °F below the minimum service temperature, whichever is lower, except that...

28 CFR 90.54 - Allocation of funds.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Allocation of funds. 90.54 Section 90.54 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) VIOLENCE AGAINST WOMEN Indian Tribal Governments... will take into account the applicants' varying needs in addressing violence against women. ...

PROPERTIES OF THE 24 DAY MODULATION IN GX 13+1 FROM NEAR-INFRARED AND X-RAY OBSERVATIONS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Corbet, Robin H. D.; Pearlman, Aaron B.; Buxton, Michelle

2010-08-10

A 24 day period for the low-mass X-ray binary (LMXB) GX 13+1 was previously proposed on the basis of seven years of RXTE All-Sky Monitor (ASM) observations and it was suggested that this was the orbital period of the system. This would make it one of the longest known orbital periods for a Galactic LMXB powered by Roche lobe overflow. We present here the results of (1) K-band photometry obtained with the SMARTS Consortium CTIO 1.3 m telescope on 68 nights over a 10 month interval; (2) continued monitoring with the RXTE ASM, analyzed using a semi-weighted power spectrum insteadmore » of the data filtering technique previously used; and (3) Swift Burst Alert Telescope (BAT) hard X-ray observations. Modulation near 24 days is seen in both the K band and additional statistically independent ASM X-ray observations. However, the modulation in the ASM is not strictly periodic. The periodicity is also not detected in the Swift BAT observations, but modulation at the same relative level as seen with the ASM cannot be ruled out. If the 24 day period is the orbital period of system, this implies that the X-ray modulation is caused by structure that is not fixed in location. A possible mechanism for the X-ray modulation is the dipping behavior recently reported from XMM-Newton observations.« less

Interaction of the Saccharomyces cerevisiae RING-domain protein Nse1 with Nse3 and the Smc5/6 complex is required for chromosome replication and stability.

PubMed

Wani, Saima; Maharshi, Neelam; Kothiwal, Deepash; Mahendrawada, Lakshmi; Kalaivani, Raju; Laloraya, Shikha

2018-06-01

Genomic stability is maintained by the concerted actions of numerous protein complexes that participate in chromosomal duplication, repair, and segregation. The Smc5/6 complex is an essential multi-subunit complex crucial for repair of DNA double-strand breaks. Two of its subunits, Nse1 and Nse3, are homologous to the RING-MAGE complexes recently described in human cells. We investigated the contribution of the budding yeast Nse1 RING-domain by isolating a mutant nse1-103 bearing substitutions in conserved Zinc-coordinating residues of the RING-domain that is hypersensitive to genotoxic stress and temperature. The nse1-103 mutant protein was defective in interaction with Nse3 and other Smc5/6 complex subunits, Nse4 and Smc5. Chromosome loss was enhanced, accompanied by a delay in the completion of replication and a modest defect in sister chromatid cohesion, in nse1-103. The nse1-103 mutant was synthetic sick with rrm3∆ (defective in fork passage through pause sites), this defect was rescued by inactivation of Tof1, a subunit of the fork protection complex that enforces pausing. The temperature sensitivity of nse1-103 was partially suppressed by deletion of MPH1, encoding a DNA-helicase. Homology modeling of the structure of the budding yeast Nse1-Nse3 heterodimer based on the human Nse1-MAGEG1 structure suggests a similar organization and indicates that perturbation of the Zn-coordinating cluster has the potential to allosterically alter structural elements at the Nse1/Nse3 interaction interface that may abrogate their association. Our findings demonstrate that the budding yeast Nse1 RING-domain organization is important for interaction with Nse3, which is crucial for completion of chromosomal replication, cohesion, and maintenance of chromosome stability.

40 CFR 205.54-2 - Sound data acquisition system.

Code of Federal Regulations, 2010 CFR

2010-07-01

... meets the “fast” dynamic requirement of a precision sound level meter indicating meter system for the... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Sound data acquisition system. 205.54... data acquisition system. (a) Systems employing tape recorders and graphic level recorders may be...

28 CFR 54.305 - Preference in admission.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Preference in admission. 54.305 Section 54.305 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF... of attendance at any educational institution or other school or entity that admits as students only...

The formation efficiency of different generations of HMXBs in the low metallicity environment of the SMC

NASA Astrophysics Data System (ADS)

Antoniou, Vallia; Zezas, Andreas; Drake, Jeremy J.; Badenes, Carles; Hong, Jaesub; SMC XVP Collaboration

2018-01-01

Nearby star-forming galaxies offer a unique environment to study the populations of young (<100 Myr) X-ray binaries, which consist of a compact object - typically a neutron star or a black hole - powered by accretion from a companion star. These systems are tracers of past populations of massive stars that heavily affect their immediate environment and parent galaxies. The Small Magellanic Cloud (SMC) is the ideal environment for population studies of young X-ray binaries by providing us with what the Milky Way cannot: A complete sample of X-ray sources within a galaxy. Using a Chandra X-ray Visionary program, we investigate the young neutron-star binary population in this low-metallicity, nearby, star-forming galaxy by reaching quiescent X-ray luminosity levels (~few times 1032 erg/s). In this talk, I will present the first measurement of the formation efficiency of high-mass X-ray binaries (HMXBs) as a function of the age of their parent stellar populations. We use three indicators of the formation efficiency of young accreting binaries in the low SMC metallicity: the number ratio of the HMXBs, N(HMXBs), to the number of OB stars, to the star-formation rate (SFR), and to the stellar mass produced during the specific star-formation burst they are associated with, all as a function of the age of their parent stellar populations. In all cases, we find that the HMXB formation efficiency increases as a function of time up to ~40—60 Myr, and then gradually decreases. The peak formation efficiency N(HMXB)/SFR is in good agreement with previous estimates of the average formation efficiency in the broad ~20—60 Myr age range, and a factor of at least ~8 and ~4 higher than the formation efficiency in earlier (~10 Myr) and later (~260 Myr) epochs. I will also present the deepest luminosity function ever recorded for a galaxy, and discuss the X-ray properties of the largest sample of extragalactic accreting pulsars as well.

46 CFR 54.05-6 - Toughness test temperatures.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 2 2012-10-01 2012-10-01 false Toughness test temperatures. 54.05-6 Section 54.05-6 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Toughness Tests § 54.05-6 Toughness test temperatures. Each toughness test must be conducted at temperatures not warmer than −20 °F or 10 °F below the...

28 CFR 54.120 - Transfers of property.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Transfers of property. 54.120 Section 54.120 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN... property. If a recipient sells or otherwise transfers property financed in whole or in part with Federal...

Paradoxical effects of injection stress and nicotine exposure experienced during adolescence on learning in a serial multiple choice (SMC) task in adult female rats.

PubMed

Renaud, Samantha M; Pickens, Laura R G; Fountain, Stephen B

2015-01-01

Nicotine exposure in adolescent rats has been shown to cause learning impairments that persist into adulthood long after nicotine exposure has ended. This study was designed to assess the extent to which the effects of adolescent nicotine exposure on learning in adulthood can be accounted for by adolescent injection stress experienced concurrently with adolescent nicotine exposure. Female rats received either 0.033 mg/h nicotine (expressed as the weight of the free base) or bacteriostatic water vehicle by osmotic pump infusion on postnatal days 25-53 (P25-53). Half of the nicotine-exposed rats and half of the vehicle rats also received twice-daily injection stress consisting of intraperitoneal saline injections on P26-53. Together these procedures produced 4 groups: No Nicotine/No Stress, Nicotine/No Stress, No Nicotine/Stress, and Nicotine/Stress. On P65-99, rats were trained to perform a structurally complex 24-element serial pattern of responses in the serial multiple choice (SMC) task. Four general results were obtained in the current study. First, learning for within-chunk elements was not affected by either adolescent nicotine exposure, consistent with past work (Pickens, Rowan, Bevins, and Fountain, 2013), or adolescent injection stress. Thus, there were no effects of adolescent nicotine exposure or injection stress on adult within-chunk learning typically attributed to rule learning in the SMC task. Second, adolescent injection stress alone (i.e., without concurrent nicotine exposure) caused transient but significant facilitation of adult learning restricted to a single element of the 24-element pattern, namely, the "violation element," that was the only element of the pattern that was inconsistent with pattern structure. Thus, adolescent injection stress alone facilitated violation element acquisition in adulthood. Third, also consistent with past work (Pickens et al., 2013), adolescent nicotine exposure, in this case both with and without adolescent

Paradoxical Effects of Injection Stress and Nicotine Exposure Experienced During Adolescence on Learning in a Serial Multiple Choice (SMC) Task in Adult Female Rats

PubMed Central

Renaud, Samantha M.; Pickens, Laura R. G.; Fountain, Stephen B.

2015-01-01

Nicotine exposure in adolescent rats has been shown to cause learning impairments that persist into adulthood long after nicotine exposure has ended. This study was designed to assess the extent to which the effects of adolescent nicotine exposure on learning in adulthood can be accounted for by adolescent injection stress experienced concurrently with adolescent nicotine exposure. Female rats received either 0.033 mg/hr nicotine (expressed as the weight of the free base) or bacteriostatic water vehicle by osmotic pump infusion on postnatal days 25-53 (P25-53). Half of the nicotine-exposed rats and half of the vehicle rats also received twice-daily injection stress consisting of intraperitoneal saline injections on P26-53. Together these procedures produced 4 groups: No Nicotine / No Stress, Nicotine / No Stress, No Nicotine / Stress, and Nicotine / Stress. On P65-99, rats were trained to perform a structurally complex 24-element serial pattern of responses in the serial multiple choice (SMC) task. Four general results were obtained in the current study. First, learning for within-chunk elements was not affected by either adolescent nicotine exposure, consistent with past work (Pickens, Rowan, Bevins, & Fountain, 2013), or adolescent injection stress. Thus, there were no effects of adolescent nicotine exposure or injection stress on adult within-chunk learning typically attributed to rule learning in the SMC task. Second, adolescent injection stress alone (i.e., without concurrent nicotine exposure) caused transient but significant facilitation of adult learning restricted to a single element of the 24-element pattern, namely, the “violation element,” that was the only element of the pattern that was inconsistent with pattern structure. Thus, adolescent injection stress alone facilitated violation element acquisition in adulthood. Third, also consistent with past work (Pickens et al., 2013), adolescent nicotine exposure, in this case both with and without

10 CFR 54.13 - Completeness and accuracy of information.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 10 Energy 2 2012-01-01 2012-01-01 false Completeness and accuracy of information. 54.13 Section 54.13 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) REQUIREMENTS FOR RENEWAL OF OPERATING LICENSES FOR NUCLEAR POWER PLANTS General Provisions § 54.13 Completeness and accuracy of information. (a...

10 CFR 54.13 - Completeness and accuracy of information.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 10 Energy 2 2011-01-01 2011-01-01 false Completeness and accuracy of information. 54.13 Section 54.13 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) REQUIREMENTS FOR RENEWAL OF OPERATING LICENSES FOR NUCLEAR POWER PLANTS General Provisions § 54.13 Completeness and accuracy of information. (a...

10 CFR 54.13 - Completeness and accuracy of information.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 10 Energy 2 2013-01-01 2013-01-01 false Completeness and accuracy of information. 54.13 Section 54.13 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) REQUIREMENTS FOR RENEWAL OF OPERATING LICENSES FOR NUCLEAR POWER PLANTS General Provisions § 54.13 Completeness and accuracy of information. (a...

10 CFR 54.13 - Completeness and accuracy of information.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 2 2010-01-01 2010-01-01 false Completeness and accuracy of information. 54.13 Section 54.13 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) REQUIREMENTS FOR RENEWAL OF OPERATING LICENSES FOR NUCLEAR POWER PLANTS General Provisions § 54.13 Completeness and accuracy of information. (a...

10 CFR 54.13 - Completeness and accuracy of information.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 10 Energy 2 2014-01-01 2014-01-01 false Completeness and accuracy of information. 54.13 Section 54.13 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) REQUIREMENTS FOR RENEWAL OF OPERATING LICENSES FOR NUCLEAR POWER PLANTS General Provisions § 54.13 Completeness and accuracy of information. (a...

46 CFR 111.54-1 - Circuit breakers.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 4 2013-10-01 2013-10-01 false Circuit breakers. 111.54-1 Section 111.54-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL... breaker must not: (1) Be dependent upon mechanical cooling to operate within its rating; or (2) Have a...

46 CFR 111.54-1 - Circuit breakers.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 4 2012-10-01 2012-10-01 false Circuit breakers. 111.54-1 Section 111.54-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL... breaker must not: (1) Be dependent upon mechanical cooling to operate within its rating; or (2) Have a...

46 CFR 111.54-1 - Circuit breakers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 4 2011-10-01 2011-10-01 false Circuit breakers. 111.54-1 Section 111.54-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL... breaker must not: (1) Be dependent upon mechanical cooling to operate within its rating; or (2) Have a...

46 CFR 111.54-1 - Circuit breakers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 4 2010-10-01 2010-10-01 false Circuit breakers. 111.54-1 Section 111.54-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING ELECTRIC SYSTEMS-GENERAL... breaker must not: (1) Be dependent upon mechanical cooling to operate within its rating; or (2) Have a...

Mechanisms responsible for two possible electrochemical reactions in Li1.2Ni0.13Mn0.54Co0.13O2 used for lithium ion batteries

NASA Astrophysics Data System (ADS)

Konishi, Hiroaki; Hirano, Tatsumi; Takamatsu, Daiko; Gunji, Akira; Feng, Xiaoliang; Furutsuki, Sho; Okumura, Takefumi; Terada, Shohei; Tamura, Kazuhisa

2018-02-01

Two electrochemical reactions are possible in regard to Li1.2Ni0.13Mn0.54Co0.13O2 (0.5Li2MnO3-0.5LiNi0.33Mn0.33Co0.33O2), viz, Li2MnO3-like and LiNi0.33Mn0.33Co0.33O2-like reactions. The open circuit potential (OCP) and changes in crystal structure during the charge-discharge process of Li1.2Ni0.13Mn0.54Co0.13O2 were investigated to clarify the mechanism responsible for the two reactions. Li2MnO3 and LiNi0.33Mn0.33Co0.33O2 were separately prepared for the investigation, and the OCPs and crystal structures in these cathodes were measured and then compared with those for Li1.2Ni0.13Mn0.54Co0.13O2. The results obtained using X-ray diffraction (XRD) indicated that two phases existed in Li1.2Ni0.13Mn0.54Co0.13O2. The changes in crystal structure of the two phases during the charge-discharge process were similar to those in Li2MnO3 and LiNi0.33Mn0.33Co0.33O2. This indicated that two phases, viz, Li2MnO3-like and LiNi0.33Mn0.33Co0.33O2-like, existed in Li1.2Ni0.13Mn0.54Co0.13O2. Li2MnO3-like, LiNi0.33Mn0.33Co0.33O2-like, and Li2MnO3-like phases were found to contribute mainly to electrochemical reactions in the low, middle, and high state of charge (SOC) ranges during the charge process from the results obtained using XRD and electrochemical measurements carried out on Li1.2Ni0.13Mn0.54Co0.13O2. In contrast, the Li2MnO3-like and LiNi0.33Mn0.33Co0.33O2-like phases mainly contributed to electrochemical reactions in the low and high SOC ranges during the discharge process. Furthermore, the high polarization and potential decay during the charge-discharge cycling of Li1.2Ni0.13Mn0.54Co0.13O2 were mainly attributed to the Li2MnO3-like phase.

Differential localization of ErbB2 in different tissues of the rat female reproductive tract: implications for the use of specific antibodies for ErbB2 analysis.

PubMed

Idris, N; Carothers Carraway, C A; Carraway, K L

2001-11-01

ErbB2 has been implicated in numerous functions, including normal and aberrant development of a variety of tissues. Although no soluble ligand has been identified for ErbB2, we have recently shown that ASGP-2, the transmembrane subunit of the cell surface glycoprotein Muc4 (also called sialomucin complex, SMC), can act as an intramembrane ligand for ErbB2 and modulate its activity. Muc4/SMC is abundantly expressed at the apical surface of most epithelia of the rat female reproductive tract. Since Muc4/SMC can interact with ErbB2 when they are expressed in the same cell and membrane, we investigated whether these two proteins are co-expressed and co-localized in tissues of the female reproductive tract. Using an anti-ErbB2 antibody from Dako, we found moderate staining at the basolateral surface of the oviduct and also around the cell membrane of the most superficial and medial layers of the stratified epithelia of the vagina. In contrast, Neomarkers neu Ab1 antibody intensely stained the apical surface of the epithelium of the oviduct and the medial and basal layers of the stratified epithelia of the vagina, substantially overlapping the distribution of Muc4/SMC. Furthermore, Muc4/SMC and ErbB2 association in different tissues of the female reproductive tract was demonstrated by co-immunoprecipitation analysis. Interestingly, phosphorylated ErbB2 detected by anti-phospho-ErbB2 is primarily present at the apical surface of the oviduct. Thus, our results show that differentially localized forms of ErbB2 are recognized by different antibodies and raise interesting questions about the nature of the different forms of ErbB2, the mechanism for differential localization, and possible functions of ErbB2 in the female reproductive tract. They also raise a cautionary note about the use of different ErbB2 antibodies for expression and localization studies. Copyright 2001 Wiley-Liss, Inc.

46 CFR 54.15-3 - Definitions (modifies Appendix 3).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 2 2010-10-01 2010-10-01 false Definitions (modifies Appendix 3). 54.15-3 Section 54.15-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PRESSURE VESSELS Pressure-Relief Devices § 54.15-3 Definitions (modifies Appendix 3). (a) Definitions applicable to...

25 CFR 273.54 - Privacy Act requirements.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 25 Indians 1 2012-04-01 2011-04-01 true Privacy Act requirements. 273.54 Section 273.54 Indians... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5 U...

25 CFR 273.54 - Privacy Act requirements.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 25 Indians 1 2013-04-01 2013-04-01 false Privacy Act requirements. 273.54 Section 273.54 Indians... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5 U...

25 CFR 273.54 - Privacy Act requirements.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 25 Indians 1 2011-04-01 2011-04-01 false Privacy Act requirements. 273.54 Section 273.54 Indians... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5 U...

25 CFR 273.54 - Privacy Act requirements.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 25 Indians 1 2014-04-01 2014-04-01 false Privacy Act requirements. 273.54 Section 273.54 Indians... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5 U...

25 CFR 273.54 - Privacy Act requirements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Privacy Act requirements. 273.54 Section 273.54 Indians... Privacy Act requirements. (a) When a contractor operates a system of records to accomplish a Bureau function, the contractor shall comply with subpart D of 43 CFR part 2 which implements the Privacy Act (5 U...

Partial blockade of Kv2.1 channel potentiates GLP-1's insulinotropic effects in islets and reduces its dose required for improving glucose tolerance in type 2 diabetic male mice.

PubMed

Sukma Rita, Rauza; Dezaki, Katsuya; Kurashina, Tomoyuki; Kakei, Masafumi; Yada, Toshihiko

2015-01-01

Glucagon-like peptide-1 (GLP-1)-based medicines have recently been widely used to treat type 2 diabetic patients, whereas adverse effects of nausea and vomiting have been documented. Inhibition of voltage-gated K(+) channel subtype Kv2.1 in pancreatic β-cells has been suggested to contribute to mild depolarization and promotion of insulin release. This study aimed to determine whether the blockade of Kv2.1 channels potentiates the insulinotropic effect of GLP-1 agonists. Kv2.1 channel blocker guangxitoxin-1E (GxTx) and GLP-1 agonist exendin-4 at subthreshold concentrations, when combined, markedly increased the insulin release and cytosolic Ca(2+) concentration ([Ca(2+)]i) in a glucose-dependent manner in mouse islets and β-cells. Exendin-4 at subthreshold concentration alone increased islet insulin release and β-cell [Ca(2+)]i in Kv2.1(+/-) mice. The [Ca(2+)]i response to subthreshold exendin-4 and GxTx in combination was attenuated by pretreatment with protein kinase A inhibitor H-89, indicating the protein kinase A dependency of the cooperative effect. Furthermore, subthreshold doses of GxTx and GLP-1 agonist liraglutide in combination markedly increased plasma insulin and improved glucose tolerance in diabetic db/db mice and NSY mice. These results demonstrate that a modest suppression of Kv2.1 channels dramatically raises insulinotropic potency of GLP-1-based drugs, which opens a new avenue to reduce their doses and associated adverse effects while achieving the same glycemic control in type 2 diabetes.