Sample records for washing cow system

  1. 33 CFR 157.124 - COW tank washing machines.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW tank washing machines. 157... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.124 COW tank washing machines. (a) COW machines must be permanently mounted in each cargo tank. (b...

  2. 33 CFR 157.124 - COW tank washing machines.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false COW tank washing machines. 157... (CONTINUED) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157...

  3. 33 CFR 157.124 - COW tank washing machines.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false COW tank washing machines. 157....124 COW tank washing machines. (a) COW machines must be permanently mounted in each cargo tank. (b) The COW machines in each tank must have sufficient nozzles with the proper diameter, working pressure...

  4. Short communication: Automatic washing of hooves can help control digital dermatitis in dairy cows.

    PubMed

    Thomsen, Peter T; Ersbøll, Annette Kjær; Sørensen, Jan Tind

    2012-12-01

    The objectives of this study were to develop and test a system for automatic washing of the hooves of dairy cows and to evaluate the effect of frequent automatic washing on the prevalence of digital dermatitis (DD). An automatic hoof washer was developed in an experimental dairy herd and tested in 6 commercial dairy herds in 2 experiments (1 and 2). In the experimental herd, automatic hoof washing resulted in cleaner hooves. In experiments 1 and 2, cows were washed after each milking on the left side only, leaving the right side unwashed as a within-cow control. In experiment 1, hooves were washed with a water and 0.4% soap solution. In experiment 2, hooves were washed with water only. In each experiment, DD was scored in a hoof-trimming chute approximately 60 d after the start of hoof washing. Data were analyzed using a generalized linear mixed model. The outcome was the DD status of each leg (DD positive or DD negative). Herd and cow within herd were included as random effects, and treatment (washing or control) was included as a fixed effect. The statistical analyses showed that the odds ratio of having DD was 1.48 in the control leg compared with the washed leg in experiment 1. In experiment 2, the odds ratio of having DD was 1.27 in the control leg compared with the washed leg. We concluded that automatic washing of hooves with water and soap can help decrease the prevalence of DD in commercial dairy herds. Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  5. 33 CFR 157.148 - COW system: Evidence for inspections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW system: Evidence for... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Inspections § 157.148 COW system... inspector evidence that the COW system has been installed in accordance with the plans accepted under § 157...

  6. 33 CFR 157.162 - Crude oil washing during a voyage.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.162 Crude oil washing during a voyage. The master of a tank vessel having a COW system under § 157.10(e), § 157.10a(a)(2), or...

  7. 33 CFR 157.156 - COW operations: Meeting manual requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW operations: Meeting manual... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.156 COW operations... COW system under §§ 157.10(e), 157.10a(a)(2), or 157.10c(b)(2) that has the Crude Oil Washing...

  8. 33 CFR 157.160 - Tanks: Ballasting and crude oil washing.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.160 Tanks... vessel having a COW system under § 157.10a(a)(2) or § 157.10c(b)(2) shall ensure that— (1) Ballast water...

  9. 33 CFR 157.130 - Crude oil washing with more than one grade of crude oil.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design... vessel having a COW system under §§ 157.10(e), 157.10a(a)(2), or 157.10c(b)(2) carries more than one grade of crude oil, the COW system must be capable of washing the cargo tanks with the grades of crude...

  10. 33 CFR 157.138 - Crude Oil Washing Operations and Equipment Manual.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and... 15 of the MARPOL 73/78. (2) A line drawing of the tank vessel's COW system showing the locations of pumps, piping, and COW machines. (3) A description of the COW system. (4) The procedure for the...

  11. 33 CFR 157.158 - COW operations: Changed characteristics.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW operations: Changed... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.158 COW operations: Changed characteristics. The COW system may be operated with characteristics that do not meet those...

  12. 33 CFR 157.155 - COW operations: General.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW operations: General. 157.155... Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.155 COW operations: General. (a) The master of a tank vessel having a COW system under § 157.10(e), § 157.10a(a)(2), or 157.10c(b)(2) shall...

  13. 33 CFR 157.170 - COW equipment: Removal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false COW equipment: Removal. 157.170... Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.170 COW equipment: Removal. (a) Whenever a deck mounted COW machine is removed from the tank, the master shall ensure that: (1) The supply...

  14. 33 CFR 157.152 - Person in charge of COW operations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Person in charge of COW... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Personnel § 157.152 Person in charge of COW operations. The owner, operator, and master of a tank vessel having a COW system under § 157.10(e...

  15. 33 CFR 157.110 - Crude Oil Washing Operations and Equipment Manual for foreign tank vessels: Submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank...: Submission. If the owner or operator of a foreign tank vessel having a COW system under § 157.10(e), § 157...

  16. Wash water recovery system

    NASA Technical Reports Server (NTRS)

    Deckman, G.; Rousseau, J. (Editor)

    1973-01-01

    The Wash Water Recovery System (WWRS) is intended for use in processing shower bath water onboard a spacecraft. The WWRS utilizes flash evaporation, vapor compression, and pyrolytic reaction to process the wash water to allow recovery of potable water. Wash water flashing and foaming characteristics, are evaluated physical properties, of concentrated wash water are determined, and a long term feasibility study on the system is performed. In addition, a computer analysis of the system and a detail design of a 10 lb/hr vortex-type water vapor compressor were completed. The computer analysis also sized remaining system components on the basis of the new vortex compressor design.

  17. 33 CFR 157.150 - Crude Oil Washing Operations and Equipment Manual: Recording information after inspections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank... used to pass the inspections under § 157.140: (1) Pressure and flow of the crude oil pumped to the COW machines. (2) Revolutions, number of cycles, and length of cycles of each COW machine. (3) Pressure and...

  18. 33 CFR 157.108 - Crude Oil Washing Operations and Equipment Manual for U.S. tank vessels: Submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank...: Submission. Before each U.S. tank vessel having a COW system under § 157.10(e), § 157.10a(a)(2), or § 157.10c... manual that meets § 157.138, to the Officer in Charge, Marine Inspection, of the zone in which the COW...

  19. Self-Contained Automated Vehicle Washing System

    DTIC Science & Technology

    2014-09-26

    SECURITY CLASSIFICATION OF: The Self Contained Automated Vehicle Washing System is a prototype that offers a reduction in the quantity of water ...supplied to the front lines by recycling wash water used in the cleaning of vehicles as well as capturing debris and other contaminates. The system also...of the warfighter to contaminates in the washing process. The System offers plug and play option for reclamation of the wash water and integration of

  20. 33 CFR 157.128 - Stripping system.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.128 Stripping system. (a) Each tank vessel having a COW system under § 157.10(e), § 157.10a(a)(2), or § 157.10c(b)(2... times the rate at which all the COW machines that are designed to simultaneously wash the bottom of the...

  1. Prototype wash water renovation system integration with government-furnished wash fixture

    NASA Technical Reports Server (NTRS)

    1984-01-01

    The requirements of a significant quantity of proposed life sciences experiments in Shuttle payloads for available wash water to support cleansing operations has provided the incentive to develop a technique for wash water renovation. A prototype wash water waste renovation system which has the capability to process the waste water and return it to a state adequate for reuse in a typical cleansing fixture designed to support life science experiments was investigated. The resulting technology is to support other developments efforts pertaining to water reclamation by serving as a pretreatment step for subsequent reclamation procedures.

  2. Wash water waste pretreatment system study

    NASA Technical Reports Server (NTRS)

    1976-01-01

    The use of real wash water had no adverse effect on soap removal when an Olive Leaf soap based system was used; 96 percent of the soap was removed using ferric chloride. Numerous chemical agents were evaluated as antifoams for synthetic wash water. Wash water surfactants used included Olive Leaf Soap, Ivory Soap, Neutrogena and Neutrogena Rain Bath Gel, Alipal CO-436, Aerosol 18, Miranol JEM, Palmeto, and Aerosol MA-80. For each type of soapy wash water evaluated, at least one antifoam capable of causing nonpersistent foam was identified. In general, the silicones and the heavy metal ions (i.e., ferric, aluminum, etc.) were the most effective antifoams. Required dosage was in the range of 50 to 200 ppm.

  3. 33 CFR 157.164 - Use of inert gas system.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.164 Use of inert gas system. (a) The master of a tank vessel having a COW system under § 157.10(e), § 157.10a(a)(2), or § 157.10c(b)(2) shall... less by volume at the locations under paragraph (a)(1) of this section. (4) During COW operations, the...

  4. Study on Intelligent Multi-concentrates Feeding System for Dairy Cow

    NASA Astrophysics Data System (ADS)

    Yan, Yinfa; Wang, Ranran; Song, Zhanhua; Yan, Shitao; Li, Fa-De

    To implement precision feeding for dairy cow, an intelligent multi-concentrates feeding system was developed. The system consists of two parts, one is precision ingredients control subsystem, the other is multi-concentrates discharge subsystem. The former controls the latter with 4 stepper motors. The precision ingredients control subsystem was designed based on Samsung S3C2440 ARM9 microprocessor and WinCE5.0 embedded operating system. The feeding system identifies the dairy cow with passive transponder using RFID (Radio frequency identification) reader. According to the differences of based diet intake and individual dairy cow milk yield, the system can automatically and quantificationally discharge 4 kinds of different concentrates on the basis of the cow identification ID. The intelligent multi-concentrates feeding system for dairy cow has been designed and implemented. According to the experiment results, the concentrate feeding error is less than 5%, the cow inditification delay time is less than 0.5s and the cow inditification error rate is less than 0.01%.

  5. Prototype Wash Water Renovation System Integration with Government-Furnished Wash Fixture

    NASA Technical Reports Server (NTRS)

    1983-01-01

    A total renovation concept for removing objectionable materials from spacecraft wash water to make the water reusable was developed. This concept included ferric chloride pretreatment to coagulate suspended solids such as soap and lint, pressure filtration, and carbon adsorption and ion exchange to remove trace dissolved organics and inorganic salts. A breadboard model which was developed to demonstrate the design adequacy of the various system components and the limits on system capacities and efficiencies.

  6. Environmental and cow-related factors affect cow locomotion and can cause misclassification in lameness detection systems.

    PubMed

    Van Nuffel, A; Van De Gucht, T; Saeys, W; Sonck, B; Opsomer, G; Vangeyte, J; Mertens, K C; De Ketelaere, B; Van Weyenberg, S

    2016-09-01

    To tackle the high prevalence of lameness, techniques to monitor cow locomotion are being developed in order to detect changes in cows' locomotion due to lameness. Obviously, in such lameness detection systems, alerts should only respond to locomotion changes that are related to lameness. However, other environmental or cow factors can contribute to locomotion changes not related to lameness and hence, might cause false alerts. In this study the effects of wet surfaces, dark environment, age, production level, lactation and gestation stage on cow locomotion were investigated. Data was collected at Institute for Agricultural and Fisheries Research research farm (Melle, Belgium) during a 5-month period. The gait variables of 30 non-lame and healthy Holstein cows were automatically measured every day. In dark environments and on wet walking surfaces cows took shorter, more asymmetrical strides with less step overlap. In general, older cows had a more asymmetrical gait and they walked slower with more abduction. Lactation stage or gestation stage also showed significant association with asymmetrical and shorter gait and less step overlap probably due to the heavy calf in the uterus. Next, two lameness detection algorithms were developed to investigate the added value of environmental and cow data into detection models. One algorithm solely used locomotion variables and a second algorithm used the same locomotion variables and additional environmental and cow data. In the latter algorithm only age and lactation stage together with the locomotion variables were withheld during model building. When comparing the sensitivity for the detection of non-lame cows, sensitivity increased by 10% when the cow data was added in the algorithm (sensitivity was 70% and 80% for the first and second algorithm, respectively). Hence, the number of false alerts for lame cows that were actually non-lame, decreased. This pilot study shows that using knowledge on influencing factors on cow

  7. DEMONSTRATION BULLETIN: SOIL WASHING SYSTEM - BIOTROL, INC.

    EPA Science Inventory

    The three component technologies of the BioTrol Soil Washing System (BSWS). Tested in the SITE demonstration were a Soil Washer (SW), and Aqueous Treatment System (ATS), and a Slurry Bio-Reactor (SBR). The Soil Washer operates on the principle that a significant fraction of the...

  8. a Sensor Based Automatic Ovulation Prediction System for Dairy Cows

    NASA Astrophysics Data System (ADS)

    Mottram, Toby; Hart, John; Pemberton, Roy

    2000-12-01

    Sensor scientists have been successful in developing detectors for tiny concentrations of rare compounds, but the work is rarely applied in practice. Any but the most trivial application of sensors requires a specification that should include a sampling system, a sensor, a calibration system and a model of how the information is to be used to control the process of interest. The specification of the sensor system should ask the following questions. How will the material to be analysed be sampled? What decision can be made with the information available from a proposed sensor? This project provides a model of a systems approach to the implementation of automatic ovulation prediction in dairy cows. A healthy well managed dairy cow should calve every year to make the best use of forage. As most cows are inseminated artificially it is of vital importance mat cows are regularly monitored for signs of oestrus. The pressure on dairymen to manage more cows often leads to less time being available for observation of cows to detect oestrus. This, together with breeding and feeding for increased yields, has led to a reduction in reproductive performance. In the UK the typical dairy farmer could save € 12800 per year if ovulation could be predicted accurately. Research over a number of years has shown that regular analysis of milk samples with tests based on enzyme linked immunoassay (ELISA) can map the ovulation cycle. However, these tests require the farmer to implement a manually operated sampling and analysis procedure and the technique has not been widely taken up. The best potential method of achieving 98% specificity of prediction of ovulation is to adapt biosensor techniques to emulate the ELISA tests automatically in the milking system. An automated ovulation prediction system for dairy cows is specified. The system integrates a biosensor with automatic milk sampling and a herd management database. The biosensor is a screen printed carbon electrode system capable of

  9. 49 CFR 393.78 - Windshield wiping and washing systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... wipers, shall have the wiper system constructed and maintained such that the performance of the wipers... operations. Windshield wiping and washing systems need not be in working condition while a commercial motor...

  10. Wash water solids removal system study

    NASA Technical Reports Server (NTRS)

    1974-01-01

    During wash water purification, surfactants tend to precipitate and foul the RO membranes, causing water flux decline and loss of salt rejection. The use of 165 to 190 ppm ferric chloride and optionally 0.25 to 1.0 ppm polymeric flocculate precipitates 92 to 96 percent of the surfactant from an Olive Leaf Soap based wash water. Crossflow filtration and pressure filtration yield good soap rejection at high water flux rates. Post-treatment of the chemically pretreated and filtered wash water with activated charcoal removes the residual soap down to an undetectable level.

  11. Review: welfare of dairy cows in continuously housed and pasture-based production systems.

    PubMed

    Arnott, G; Ferris, C P; O'Connell, N E

    2017-02-01

    There is increasing interest in the use of continuous housing systems for dairy cows, with various reasons put forward to advocate such systems. However, the welfare of dairy cows is typically perceived to be better within pasture-based systems, although such judgements are often not scientifically based. The aim of this review was to interrogate the existing scientific literature to compare the welfare, including health, of dairy cows in continuously housed and pasture-based systems. Although summarising existing work, knowledge gaps and directions for future research are also identified. The scope of the review is broad, examining relevant topics under three main headings; health, behaviour and physiology. Regarding health, cows on pasture-based systems had lower levels of lameness, hoof pathologies, hock lesions, mastitis, uterine disease and mortality compared with cows on continuously housed systems. Pasture access also had benefits for dairy cow behaviour, in terms of grazing, improved lying/resting times and lower levels of aggression. Moreover, when given the choice between pasture and indoor housing, cows showed an overall preference for pasture, particularly at night. However, the review highlighted the need for a deeper understanding of cow preference and behaviour. Potential areas for concern within pasture-based systems included physiological indicators of more severe negative energy balance, and in some situations, the potential for compromised welfare with exposure to unpredictable weather conditions. In summary, the results from this review highlight that there remain considerable animal welfare benefits from incorporating pasture access into dairy production systems.

  12. DEMONSTRATION BULLETIN: SOIL/SEDIMENT WASHING SYSTEM BERGMANN USA

    EPA Science Inventory

    The Bergmann USA Soil/Sediment Washing System is a waste minimization technique designed to separate or "partition" soils and sediments by grain size and density. In this water-based volume reduction process, hazardous contaminants are concentrated into a small residual portion...

  13. DEMONSTRATION BULLETIN: BESCORP SOIL WASHING SYSTEM ALASKAN BATTERY ENTERPRISES SITE - BRICE ENVIRONMENTAL SERVICES CORPORATION

    EPA Science Inventory

    The BESCORP Soil Washing System is an aqueous volume reduction system that utilizes trommel agitation, high-pressure washing, sizing, and density separation to remove lead, lead compounds, and battery casing chips from soil contaminated by broken lead batteries. The basic concept...

  14. Dissecting the COW

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Linstadt, E.

    1985-10-01

    The COW, or Console On Wheels, is the primary operator interface to the SLC accelerator control system. A hardware and software description of the COW, a microcomputer based system with a color graphics display output and touchpanel and knob inputs, is given. The ease of development and expandability, due to both the modular nature of the hardware and the multitasking, interrupt driven software running in the COW, are described. Integration of the COW into the SLCNET communications network and SLC Control system is detailed.

  15. Dissecting the COW

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Linstadt, E.

    1985-04-01

    The COW, or Console On Wheels, is the primary operator interface to the SLC accelerator control system. A hardware and software description of the COW, a microcomputer based system with a color graphics display output and touch-panel and knob inputs, is given. The ease of development and expandability, due to both the modular nature of the hardware and the multitasking, interrupt driven software running in the COW, are described. Integration of the COW into the SLCNET communications network and SLC Control system is detailed.

  16. Uterine and systemic inflammation influences ovarian follicular function in postpartum dairy cows

    PubMed Central

    Sá Filho, Ocilon G.; Absalon-Medina, Victor A.; Schneider, Augusto; Butler, W. R.; Gilbert, Robert O.

    2017-01-01

    The objective of this study was to determine the effects of uterine and systemic inflammatory responses to uterine bacterial contamination at calving in dairy cows on the growth and ovulatory outcomes of the first dominant follicle postpartum. Ovulatory capability of the first dominant follicle postpartum was predicted in 53 multiparous cows by using a combination of follicle growth characteristics and circulating estradiol concentrations. Endotoxin levels were assayed in follicular fluid samples that were aspirated the day after ovulatory outcome prediction. Plasma levels of haptoglobin, a proinflammatory acute phase protein, and paraoxonase, a negative acute phase protein were determined. Uterine bacteria and inflammation were evaluated in three uterine fluid samples from each cow collected on the day of calving, the day after follicle aspiration, and at 35 days postpartum. Cows that had a strong initial uterine inflammatory response (robust recruitment of polymorphonuclear leukocytes of ≥ 35% and cows with uterine pH < 8.5 on the day of calving) were more likely to have an ovulatory first dominant follicle. Follicular fluid endotoxin levels were higher in non-ovulatory cows compared with ovulatory cows. Endotoxin levels in circulation were not different between ovulatory groups but were higher prepartum than on day 7 and 14 postpartum. Systemic inflammation characterized by elevated haptoglobin concentrations was higher in non-ovulatory cows despite similar bacterial contamination and circulating endotoxin levels. Paraoxonase activity in follicular fluid was significantly associated with the paraoxonase activity in plasma, however, plasma paraoxonase concentrations were not different between non-ovulatory and ovulatory cows. Cows with a higher uterine bacterial load on the day of calving had slower ovarian follicle growth. In summary, a robust uterine inflammatory response on the day of calving was positively associated with ovarian function while elevated

  17. Characterization of Dutch dairy farms using sensor systems for cow management.

    PubMed

    Steeneveld, W; Hogeveen, H

    2015-01-01

    To improve cow management in large dairy herds, sensors have been developed that can measure physiological, behavioral, and production indicators on individual cows. Recently, the number of dairy farms using sensor systems has increased. It is not known, however, to what extent sensor systems are used on dairy farms, and the reasons why farmers invest or not in sensor systems are unclear. The first objective of this study was to give an overview of the sensor systems currently used in the Netherlands. The second objective was to investigate the reasons for investing or not investing in sensor systems. The third objective was to characterize farms with and without sensor systems. A survey was developed to investigate first, the reasons for investing or not in sensor systems and, then, how the sensor systems are used in daily cow management. The survey was sent to 1,672 Dutch dairy farmers. The final data set consisted of 512 dairy farms (response rate of 30.6%); 202 farms indicated that they had sensor systems and 310 farms indicated that they did not have sensor systems. A wide variety of sensor systems was used on Dutch dairy farms; those for mastitis detection and estrus detection were the most-used sensor systems. The use of sensor systems was different for farms using an automatic milking system (AMS) and a conventional milking system (CMS). Reasons for investing were different for different sensor systems. For sensor systems attached to the AMS, the farmers made no conscious decision to invest: they answered that the sensors were standard in the AMS or were bought for reduced cost with the AMS. The main reasons for investing in estrus detection sensor systems were improving detection rates, gaining insights into the fertility level of the herd, improving profitability of the farm, and reducing labor. Main reasons for not investing in sensor systems were economically related. It was very difficult to characterize farms with and without sensor systems. Farms

  18. The partitioning and modelling of pesticide parathion in a surfactant-assisted soil-washing system.

    PubMed

    Chu, W; Chan, K H; Choy, W K

    2006-07-01

    Soil sorption of organic pollutants has long been a problematic in the soil washing process because of its durability and low water solubility. This paper discussed the soil washing phenomena over a wide range of parathion concentrations and several soil samples at various fractions of organic content (foc) levels. When parathion dosage is set below the water solubility, washing performance is stable for surfactant concentrations above critical micelle concentration (cmc) and it is observed that more than 90% of parathion can be washed out when dosage is five times lower than the solubility limit. However, such trends change when non-aqueous phase liquids (NAPL) is present in the system. Parathion extraction depends very much on the surfactant dosage but is not affected by the levels of foc in the system. In between the extreme parathion dosage, a two-stage pattern is observed in these boundary regions. Washing performance is first increased with additional surfactant, but the increase slows down gradually since the sorption sites are believed to be saturated by the huge amount of surfactant in the system. A mathematical model has included foc to demonstrate such behavior and this can be used as a prediction for extraction.

  19. 21 CFR 1250.87 - Wash water.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Wash water. 1250.87 Section 1250.87 Food and Drugs... Sanitation Facilities and Conditions on Vessels § 1250.87 Wash water. Where systems installed on vessels for wash water, as defined in § 1250.3(n), do not comply with the requirements of a potable water system...

  20. 21 CFR 1250.87 - Wash water.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Wash water. 1250.87 Section 1250.87 Food and Drugs... Sanitation Facilities and Conditions on Vessels § 1250.87 Wash water. Where systems installed on vessels for wash water, as defined in § 1250.3(n), do not comply with the requirements of a potable water system...

  1. 21 CFR 1250.87 - Wash water.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Wash water. 1250.87 Section 1250.87 Food and Drugs... Sanitation Facilities and Conditions on Vessels § 1250.87 Wash water. Where systems installed on vessels for wash water, as defined in § 1250.3(n), do not comply with the requirements of a potable water system...

  2. 21 CFR 1250.87 - Wash water.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Wash water. 1250.87 Section 1250.87 Food and Drugs... Sanitation Facilities and Conditions on Vessels § 1250.87 Wash water. Where systems installed on vessels for wash water, as defined in § 1250.3(n), do not comply with the requirements of a potable water system...

  3. 21 CFR 1250.87 - Wash water.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Wash water. 1250.87 Section 1250.87 Food and Drugs... Sanitation Facilities and Conditions on Vessels § 1250.87 Wash water. Where systems installed on vessels for wash water, as defined in § 1250.3(n), do not comply with the requirements of a potable water system...

  4. Laboratory evaluation of the 3-bowl system used for washing-up eating utensils in the field.

    PubMed

    Hargreaves, Joanna S

    2006-01-01

    A 3-bowl system is used for washing-up eating utensils on many expeditions when running water is not available. The utensils are washed in the first bowl until they are visibly clean, rinsed in the second bowl, and disinfected in the third bowl. The objective of this study was to evaluate the efficacy of this system in reducing bacterial loads on contaminated utensils and to compare it with alternative washing-up methods. Different washing-up systems were tested with a simulated dish washing of 5 contaminated mess tins followed by 5 uncontaminated mess tins. Porridge was used to simulate food residue and was mixed with Escherichia coli to produce bacterial contamination. Reduction of bacterial load on the mess tins was measured, as were subjective observations regarding the various systems. Bacterial load on contaminated tins is reduced when the 3-bowl system is used. Uncontaminated tins become contaminated in bowl 1, but this is then reduced in subsequent bowls. Disinfectant use, especially bleach, produced a marked reduction in bacterial load on contaminated and uncontaminated tins when used in bowl 2. Detergent is needed to remove grease, and a final rinse removes the smell of disinfectant. Overall, the most effective washing-up system in the laboratory was removal of most food residue with detergent in bowl 1, finish washing with bleach until visibly clean in bowl 2, and a final rinse in drinkable water in bowl 3. This system has advantages over the established 3-bowl system by getting mess tins clean more easily, killing potentially harmful bacteria, and removing the smell and taste of disinfectant.

  5. Technical note: Validation of a commercial system for the continuous and automated monitoring of dairy cow activity.

    PubMed

    Tullo, E; Fontana, I; Gottardo, D; Sloth, K H; Guarino, M

    2016-09-01

    Current farm sizes do not allow the precise identification and tracking of individual cows and their health and behavioral records. Currently, the application of information technology within intensive dairy farming takes a key role in proper routine management to improve animal welfare and to enhance the comfort of dairy cows. An existing application based on information technology is represented by the GEA CowView system (GEA Farm Technologies, Bönen, Germany). This system is able to detect and monitor animal behavioral activities based on positioning, through the creation of a virtual map of the barn that outlines all the areas where cows have access. The aim of this study was to validate the accuracy, sensitivity, and specificity of data provided by the CowView system. The validation was performed by comparing data automatically obtained from the CowView system with those obtained by a manual labeling procedure performed on video recordings. Data used for the comparisons were represented by the zone-related activities performed by the selected dairy cows and were classified into 2 categories: activity and localization. The duration in seconds of each of the activities/localizations detected both with the manual labeling and with the automated system were used to evaluate the correlation coefficients among data; and subsequently the accuracy, sensitivity, specificity, and positive and negative predictive values of the automated monitoring system were calculated. The results of this validation study showed that the CowView automated monitoring system is able to identify the cow localization/position (alley, trough, cubicles) with high reliability in relation to the zone-related activities performed by dairy cows (accuracy higher than 95%). The results obtained support the CowView system as an innovative potential solution for the easier management of dairy cows. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  6. Impact of cow strain and concentrate supplementation on grazing behaviour, milk yield and metabolic state of dairy cows in an organic pasture-based feeding system.

    PubMed

    Heublein, C; Dohme-Meier, F; Südekum, K-H; Bruckmaier, R M; Thanner, S; Schori, F

    2017-07-01

    As ruminants are able to digest fibre efficiently and assuming that competition for feed v. food use would intensify in the future, cereals and other field crops should primarily be destined to cover the dietary needs of humans and monogastric animals such as poultry and pigs. Farming systems with a reduced or absent concentrate supplementation, as postulated by organic agriculture associations, require adapted dairy cows. The aim of this experiment was to examine the impact of concentrate supplementation on milk production, grazing and rumination behaviour, feed intake, physical activity and blood traits with two Holstein-Friesian cow strains and to conclude the consequences for sustainable and organic farming. The experiment was a cross-over study and took place on an organic farm in Switzerland. In all, 12 Swiss Holstein-Friesian (HCH) cows and 12 New Zealand Holstein-Friesian (HNZ) cows, which were paired according to lactation number, days in milk and age for primiparous cows, were used. All cows grazed full time and were supplemented either with 6 kg/day of a commercial, organic cereal-grain mix or received no supplement. After an adaptation period of 21 days, a measurement period of 7 days followed, where milk yield and composition, pasture dry matter intake estimated with the n-alkane double-indicator technique, physical activity based on pedometer measurements, grazing behaviour recorded by automatic jaw movement recorder and blood samples were investigated. Non-supplemented cows had a lower milk yield and supplemented HCH cows produced more milk than supplemented HNZ cows. Grazing time and physical activity were greater for non-supplemented cows. Supplementation had no effect on rumination behaviour, but HNZ cows spent longer ruminating compared with HCH cows. Pasture dry matter intake decreased with the concentrate supplementation. Results of blood analysis did not indicate a strong negative energy balance for either non-supplemented or supplemented cows

  7. A novel reverse-osmosis wash water recycle system for manned space stations

    NASA Technical Reports Server (NTRS)

    Ray, R. J.; Babcock, W. C.; Barss, R. P.; Andrews, T. A.; Lachapelle, E. D.

    1984-01-01

    The preliminary development of a wash water recycle system utilizing an inside-skinned hollow-fiber membrane is described. This module configuration is based on tube-side feed and is highly resistant to fouling with a minimum of pretreatment. During an ongoing research program for NASA, these modules were operated on actual wash waters with no significant fouling for a period of 40 days. Due to the tube-side-feed flow in these hollow-fiber membranes, the fibers themselves become the pressure vessels, allowing the development of extremely lightweight membrane modules. During the NASA research program, a pre-prototype membrane module capable of processing 6 gallons per day of wash water at 97 percent recovery was developed that can be dry-stored and that weighs 120 g.

  8. Short communication: A reproductive tract scoring system to manage fertility in lactating dairy cows.

    PubMed

    Young, C D; Schrick, F N; Pohler, K G; Saxton, A M; Di Croce, F A; Roper, D A; Wilkerson, J B; Edwards, J L

    2017-07-01

    We developed a reproductive tract size and position score (SPS) system as a reproductive management tool to identify lactating dairy cows with decreased fertility. This system, relying solely on transrectal palpation, considers the size (cervical and uterine) and position of the reproductive tract relative to the pelvis. Cows undergoing pre-breeding exams were identified as having reproductive tracts that were small (SPS1), medium (SPS2), or large (SPS3). Cows designated SPS1 had small and compact uterine horns that rested within the pelvic cavity; SPS2 cows had reproductive tracts that were intermediate in cervical and uterine horn diameter, with longer uterine horns resting partially outside the pelvic cavity; and SPS3 cows had reproductive tracts that were larger and rested mostly outside the pelvic cavity. Cows that were SPS1 had a higher rate of pregnancy per artificial insemination (43.3 ± 3.7%) than cows that were SPS2 (36.9 ± 3.6%) or SPS3 (27.7 ± 4.3%). The percentage of cows with an SPS2 score differed in pregnancies per artificial insemination compared with SPS3 cows. The average days in milk was similar for SPS1, SPS2, and SPS3 cows (104.3 ± 3.5, 98.4 ± 3.4, and 94.7 ± 7.7, respectively). Ultrasound measurements of the uterine horn and cervical diameter, and length measurements of the uterine horns, cervix, and vagina confirmed differences among the SPS groups derived by transrectal palpation. The ease with which transrectal palpation can be used to determine the size and position of the reproductive tract attests to the relevance and usefulness of this scoring system to identify less fertile lactating dairy cows. The ability to do so with ease provides an opportunity to make economically relevant management decisions and maximize reproductive efficiency in a given herd. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  9. 33 CFR 157.128 - Stripping system.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.128 Stripping...) must have a stripping system that is designed to remove crude oil from— (1) Each cargo tank at 1.25...

  10. 33 CFR 157.128 - Stripping system.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.128 Stripping...) must have a stripping system that is designed to remove crude oil from— (1) Each cargo tank at 1.25...

  11. 33 CFR 157.128 - Stripping system.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.128 Stripping...) must have a stripping system that is designed to remove crude oil from— (1) Each cargo tank at 1.25...

  12. 33 CFR 157.128 - Stripping system.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) POLLUTION RULES FOR THE PROTECTION OF THE MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.128 Stripping...) must have a stripping system that is designed to remove crude oil from— (1) Each cargo tank at 1.25...

  13. Dairy cow breed interacts with stocking rate in temperate pasture-based dairy production systems.

    PubMed

    Spaans, O K; Macdonald, K A; Lancaster, J A S; Bryant, A M; Roche, J R

    2018-05-01

    Economic optimum stocking rates for grazing dairy systems have been defined by accounting for the pasture production potential of the farm [t of dry matter (DM)/ha], the amount of feed imported from outside the farm (t of DM/ha), and the size of the cow (kg). These variables were combined into the comparative stocking rate [CSR; kg of body weight (BW)/t of feed DM available] measure. However, CSR assumes no effect of cow genetics beyond BW, and there is increasing evidence of within-breed differences in residual feed intake and between-breed differences in the gross efficiency with which cows use metabolizable energy for milk production. A multiyear production system experiment was established to determine whether Jersey (J) and Holstein-Friesian (HF) breeds performed similarly at the same CSR. Fifty-nine J cows and 51 HF cows were randomly allocated to 1 of 2 CSR in a 2 × 2 factorial arrangement; systems were designed to have a CSR of either 80 or 100 kg of BW/t of feed DM (J-CSR80, J-CSR100, HF-CSR80, and HF-CSR100 treatment groups). Data were analyzed for consistency of farmlet response over years using ANOVA procedures, with year and farmlet as fixed effects and the interaction of farmlet with year as a random effect. The collated biological data and financial data extracted from a national economic database were used to model the financial performance for the different breed and CSR treatments. On average, annual and individual season pasture DM production was greater for the J farmlets and was less in the CSR100 treatment; however, the effect of CSR was primarily driven by a large decline in pasture DM production in the HF-CSR100 treatment (breed × CSR interaction). This interaction in feed availability resulted in a breed × CSR interaction for the per-cow and per-hectare milk production variables, with HF cows producing more milk and milk components per cow in the CSR80 treatment but the same amount as the J cows in the CSR100 treatment. On a per

  14. Short communication: Characterizing metabolic and oxidant status of pastured dairy cows postpartum in an automatic milking system.

    PubMed

    Elischer, M F; Sordillo, L M; Siegford, J M; Karcher, E L

    2015-10-01

    The periparturient period represents a stressful time for dairy cows as they transition from late gestation to early lactation. Undesirable fluctuations in metabolites and impaired immune defense mechanisms near parturition can severely affect cow health and have residual effects on performance and longevity. Metabolic and oxidative stress profiles of multiparous and primiparous dairy cows in traditional parlor and feeding systems are well characterized, but status of these profiles in alternative management systems, such as grazing cows managed with an automatic milking system (AMS), are poorly characterized. Therefore, the objective of this case study was to characterize the metabolic and oxidant status of pastured cows milked with an AMS. It was hypothesized that primiparous and multiparous cows milked with an AMS would experience changes in oxidative and metabolic status after parturition; however, these changes would not impair cow health or production. Blood was collected from 14 multiparous and 8 primiparous Friesian-cross dairy cows at 1, 7, 14, and 21 d relative to calving for concentrations of insulin, glucose, nonesterified fatty acids (NEFA), β-hydroxybutyrate, reduced glutathione, oxidized glutathione, and antioxidant potential. Milk production and milking frequency data were collected postpartum. Milk production differed on d 7 and 14 between primiparous and multiparous cows and frequency was not affected by parity. Primiparous cows had higher levels of glucose than multiparous cows. No differences in insulin, NEFA, or β-hydroxybutyrate concentrations were noted between multiparous and primiparous cows postpartum, though days relative to calving significantly affected insulin and NEFA. Primiparous cows also had higher antioxidant potential than multiparous cows during the postpartum period. Results from this study show that, although responses were within expected ranges, periparturient multiparous cows responded differently than periparturient

  15. A comparison of native tallgrass prairie and plains bluestem forage systems for cow-calf production in the southern great plains.

    PubMed

    Coleman, S W; Phillips, W A; Volesky, J D; Buchanan, D

    2001-07-01

    The objective of this study was to compare an introduced warm-season perennial grass (plains bluestem, Bothriochloa ischaemum) to native tallgrass prairie for cow-calf production. Three systems were used, two based on tallgrass prairie with two different forms of protein supplementation and one based on plains bluestem as the primary forage. The systems were as follows: 1) native tallgrass prairie with pelleted oilseed meal as the winter protein supplement (native-control); 2) native tallgrass prairie with limited access to wheat pasture as the winter protein supplement (native-wheat); and 3) plains bluestem with limited access to wheat pasture as the protein supplement (bluestem-wheat). Oilseed meal protein supplements were fed twice weekly. Cows grazing wheat pasture were allowed 6 h of grazing twice weekly. Ninety-nine cows per year were used over the 3-yr study. Cows were sired by either Charolais, Gelbvieh, Angus, or Hereford bulls out of commercial Angus-Hereford dams. Calves were sired by Simmental bulls. Calving and weaning rate increased over time but did not differ among systems or breed types. System did not influence the size or body condition score of cows or the performance of calves, but changes in the weight and condition scores of cows were greater on either native system than on the bluestem-wheat system. Cows from Charolais and Gelbvieh bulls were taller (P < 0.05), and heavier (P < 0.05), and weaned heavier (P < 0.05) calves than cows from Angus or Hereford bulls. The weight of cows on the bluestem-wheat system tended to decrease over time, whereas cows grazing on the native systems tended to gain weight over time. The native-control system was the most profitable system based on cow production. If excess hay produced from the bluestem-wheat system was sold as a cash crop, then this system was the most profitable. In general, we conclude that limit-grazing wheat pasture is a viable alternative to oilseed meal as protein supplement for wintering

  16. Reproduction, mastitis, and body condition of seasonally calved Holstein and Jersey cows in confinement or pasture systems.

    PubMed

    Washburn, S P; White, S L; Green, J T; Benson, G A

    2002-01-01

    Dairy cows in confinement and pasture-based feeding systems were compared across four spring-calving and three fall-calving replicates for differences in reproduction, mastitis, body weights, and body condition scores. Feeding systems and replicates included both Jersey and Holstein cows. Cows in confinement were fed a total mixed ration, and cows on pasture were supplemented with concentrates and provided baled hay or haylage when pasture supply was limiting. Breeding periods were for 75 d in spring or fall. Reproductive performance did not differ significantly due to feeding system or season. Jerseys had higher conception rates (59.6 vs. 49.5 +/- 3.3%) and higher percentages of cows pregnant in 75 d (78.1 vs. 57.9 +/- 3.9%) than Holsteins. Cows in confinement had 1.8 times more clinical mastitis and eight times the rate of culling for mastitis than did cows on pasture. Jerseys had half as many clinical cases of mastitis per cow as Holsteins. Only 41 +/- 5% of confinement Holsteins remained for a subsequent lactation, starting within the defined calving season compared with 51 +/- 5% of pastured Holsteins and 71 and 72 +/- 5% of Jerseys, respectively. Body weights and condition scores were generally higher for confinement cows than pastured cows, and Jerseys had higher condition scores and lower body weights than Holsteins. In summary, pastured cows had fewer clinical cases of mastitis, lower body condition scores, and lower body weights than confinement cows. Holsteins were less likely to rebreed, had more mastitis, higher culling rates, and lower body condition scores than Jerseys.

  17. Dairy cows welfare quality in tie-stall housing system with or without access to exercise.

    PubMed

    Popescu, Silvana; Borda, Cristin; Diugan, Eva Andrea; Spinu, Marina; Groza, Ioan Stefan; Sandru, Carmen Dana

    2013-06-01

    Tie-stall housing of dairy cows is used extensively worldwide, despite of the welfare concerns regarding the restriction of voluntary movement and limitation of expression of the cows' natural behaviour. The aim of this study was to compare the welfare quality of dairy cows kept in two types of tie-stall housing systems: with regular outdoor exercise and without access to exercise. In addition, the study investigated the relationship between different welfare measures of dairy cows kept in tie-stalls. 3,192 lactating cows were assessed using the Welfare Quality® assessment protocol for cattle in 80 commercial dairy farms, half of the farms providing outdoor access for the animals to exercise. The descriptive statistical indicators were determined for the assessed measures and for the welfare criteria and principle scores. The data obtained in the two housing types were compared and the correlation coefficients were calculated between the different welfare measures. The significant differences found between the two housing systems for the majority of the animal based measures indicate the positive effect of exercise on the welfare of tethered cows. Many of the animal welfare parameters correlated with each other. For the farms allowing the cows' turnout in a paddock, pasture or both, the mean scores for the welfare criteria and principles were higher than for the farms with permanent tethering of the cows, except the criteria absence of prolonged hunger and expression of social behaviours. The lowest scores were obtained for the criterion positive emotional state, in both housing systems. With regard to the overall classification, none of the farms were considered excellent. In the not classified category were only farms with all-year-round tethering of the animals and in the enhanced category only farms where the cows had outdoor access. The welfare quality of the investigated dairy cows was significantly better in the tie-stall farms which allow exercise for cows

  18. Hand Washing

    MedlinePlus

    ... study, only 58% of female and 48% of male middle- and high-school students washed their hands after using the bathroom. Yuck! How to Wash Your Hands Correctly There's a right way to wash your hands. Follow these simple ...

  19. Washing Out the Competition

    NASA Technical Reports Server (NTRS)

    2001-01-01

    AJT Associates, Inc. (AJT) worked with NASA to develop a revolutionary ozone-based laundry system. AJT's TecH2Ozone(R) wash system presents its customers with an energy-efficient, cost-effective, and environmentally safe way to perform commercial laundering. TecH2Ozone significantly reduces the amount of water and chemical used as compared to traditional commercial laundry systems. This reduction has resulted in lower cost and shorter wash cycles. And due to the reduced use of chemicals, a significant portion of the rinse water is recycled back into the system for reuse. TecH2Ozone customers, such as hotels and other large commercial laundry facilities, have felt the benefits of this equipment. Because of the reduced cycle times, fewer washers are needed and there is a notable increase in the cleanliness of the laundry. The reduction in chemical residues is a boon to customers with allergies and those prone to skin irritation from chemicals retained in regular laundry. AJT Associates, Inc. (AJT) worked with NASA to develop a revolutionary ozone-based laundry system. AJT's TecH2Ozone(R) wash system presents its customers with an energy-efficient, cost-effective, and environmentally safe way to perform commercial laundering.

  20. Grazing Soybean to Increase Voluntary Cow Traffic in a Pasture-based Automatic Milking System

    PubMed Central

    Clark, C. E. F.; Horadagoda, A.; Kerrisk, K. L.; Scott, V.; Islam, M. R.; Kaur, R.; Garcia, S. C.

    2014-01-01

    Pasture-based automatic milking systems (AMS) require cow traffic to enable cows to be milked. The interval between milkings can be manipulated by strategically allocating pasture. The current experiment investigated the effect of replacing an allocation of grazed pasture with grazed soybean (Glycine max) with the hypothesis that incorporating soybean would increase voluntary cow traffic and milk production. One hundred and eighty mixed age, primiparous and multiparous Holstein-Friesian/Illawarra cows were randomly assigned to two treatment groups (n = 90/group) with a 2×2 Latin square design. Each group was either offered treatments of kikuyu grass (Pennisetum clandestinum Hoach ex Chiov.) pasture (pasture) or soybean from 0900 h to 1500 h during the experimental period which consisted of 2 periods of 3 days following 5 days of training and adaptation in each period with groups crossing over treatments after the first period. The number of cows trafficking to each treatment was similar together with milk yield (mean ≈18 L/cow/d) in this experiment. For the cows that arrived at soybean or pasture there were significant differences in their behaviour and consequently the number of cows exiting each treatment paddock. There was greater cow traffic (more cows and sooner) exiting pasture allocations. Cows that arrived at soybean stayed on the allocation for 25% more time and ate more forage (8.5 kg/cow/d/allocation) relative to pasture (4.7 kg/cow/d/allocation). Pasture cows predominantly replaced eating time with rumination. These findings suggest that replacing pasture with alternative grazeable forages provides no additional incentive to increase voluntary cow traffic to an allocation of feed in AMS. This work highlights the opportunity to increase forage intakes in AMS through the incorporation of alternative forages. PMID:25049970

  1. Invited review: The impact of automatic milking systems on dairy cow management, behavior, health, and welfare.

    PubMed

    Jacobs, J A; Siegford, J M

    2012-05-01

    Over the last 100 yr, the dairy industry has incorporated technology to maximize yield and profit. Pressure to maximize efficiency and lower inputs has resulted in novel approaches to managing and milking dairy herds, including implementation of automatic milking systems (AMS) to reduce labor associated with milking. Although AMS have been used for almost 20 yr in Europe, they have only recently become more popular in North America. Automatic milking systems have the potential to increase milk production by up to 12%, decrease labor by as much as 18%, and simultaneously improve dairy cow welfare by allowing cows to choose when to be milked. However, producers using AMS may not fully realize these anticipated benefits for a variety of reasons. For example, producers may not see a reduction in labor because some cows do not milk voluntarily or because they have not fully or efficiently incorporated the AMS into their management routines. Following the introduction of AMS on the market in the 1990s, research has been conducted examining AMS systems versus conventional parlors focusing primarily on cow health, milk yield, and milk quality, as well as on some of the economic and social factors related to AMS adoption. Additionally, because AMS rely on cows milking themselves voluntarily, research has also been conducted on the behavior of cows in AMS facilities, with particular attention paid to cow traffic around AMS, cow use of AMS, and cows' motivation to enter the milking stall. However, the sometimes contradictory findings resulting from different studies on the same aspect of AMS suggest that differences in management and farm-level variables may be more important to AMS efficiency and milk production than features of the milking system itself. Furthermore, some of the recommendations that have been made regarding AMS facility design and management should be scientifically tested to demonstrate their validity, as not all may work as intended. As updated AMS

  2. [Massive transfusion of washed red blood cells: acid-base and electrolyth changes for different wash solutions].

    PubMed

    Sümpelmann, R; Schürholz, T; Marx, G; Ahrenshop, O; Zander, R

    2003-09-01

    The composition of normal saline (NaCl), the standard wash solution for cell saver autotransfusion, is considerably different from physiologic plasma values in small infants. Therefore, we investigated acid-base and electrolyte changes during massive cell saver autotransfusion with different wash solutions in young pigs. After approval by the animal protection authorities 15 young pigs (weight 10.6 +/- 1.1 kg, blood volume 848 +/- 88 ml, mean+/-SD) underwent 15 cycles of cell saver autotransfusion (Haemolite 2plus, Haemonetics). For each cycle, 100 ml arterial blood was withdrawn, washed with NaCl, physiologic multielectrolyte solution (PME, V Infusionslösung 296 mval Elektrolyte, Baxter) or physiologic erythrocyte protection solution (PEP, 3.2 % gelatine, pH 7.40, cHCO3 24 mmol/l), and then retransfused. Analyses of acid-base, electrolyte, and hematologic parameters were performed for systemic and washed blood samples. For NaCl there was a progressive decrease in systemic pH, HCO3 and base excess (BE) and an increase in chloride values (Cl) (p < 0.05). Use of PME slightly decreased pH (n. s.), whereas HCO3, BE and Cl remained stable. PEP slightly increased pH, HCO3 and BE, and decreased Cl (n. s.). Free hemoglobin increased in NaCl and PME (p < 0.05) and was below baseline in PEP (n. s.). Lactic acid course was comparable in all groups. The use of NaCl as wash solution for massive autotransfusion resulted in metabolic acidosis caused by dilution of HCO3 and increased Cl values. Fewer systemic acid-base and electrolyte changes were observed, when blood was washed with PME or PEP. The decreased hemoglobin release with PEP is possibly due to a gelatine specific electrostatic surface coating of erythrocyte membranes. For massive transfusion of washed red blood cells, physiologic multielectrolyte solution and physiologic erythrocyte protection solution should be preferred to NaCl, especially for small infants.

  3. 3. VIEW LOOKING NORTH AT CHINA WASH FLUME SHOWING WASH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW LOOKING NORTH AT CHINA WASH FLUME SHOWING WASH - San Carlos Irrigation Project, China Wash Flume, Main (Florence-Case Grande) Canal at Station 137+00, T4S, R10E, S14, Coolidge, Pinal County, AZ

  4. Dairy cows welfare quality in tie-stall housing system with or without access to exercise

    PubMed Central

    2013-01-01

    Background Tie-stall housing of dairy cows is used extensively worldwide, despite of the welfare concerns regarding the restriction of voluntary movement and limitation of expression of the cows’ natural behaviour. The aim of this study was to compare the welfare quality of dairy cows kept in two types of tie-stall housing systems: with regular outdoor exercise and without access to exercise. In addition, the study investigated the relationship between different welfare measures of dairy cows kept in tie-stalls. Methods 3,192 lactating cows were assessed using the Welfare Quality® assessment protocol for cattle in 80 commercial dairy farms, half of the farms providing outdoor access for the animals to exercise. The descriptive statistical indicators were determined for the assessed measures and for the welfare criteria and principle scores. The data obtained in the two housing types were compared and the correlation coefficients were calculated between the different welfare measures. Results The significant differences found between the two housing systems for the majority of the animal based measures indicate the positive effect of exercise on the welfare of tethered cows. Many of the animal welfare parameters correlated with each other. For the farms allowing the cows’ turnout in a paddock, pasture or both, the mean scores for the welfare criteria and principles were higher than for the farms with permanent tethering of the cows, except the criteria absence of prolonged hunger and expression of social behaviours. The lowest scores were obtained for the criterion positive emotional state, in both housing systems. With regard to the overall classification, none of the farms were considered excellent. In the not classified category were only farms with all-year-round tethering of the animals and in the enhanced category only farms where the cows had outdoor access. Conclusions The welfare quality of the investigated dairy cows was significantly better in the

  5. Pasture-derived greenhouse gas emissions in cow-calf production systems.

    PubMed

    Chiavegato, M B; Powers, W J; Carmichael, D; Rowntree, J E

    2015-03-01

    There is a lack of information regarding carbon dioxide (CO), methane (CH), and nitrous oxide (NO) emissions from pasture soils and the effects of grazing. The objective of this study was to quantify greenhouse gas (GHG) fluxes from pasture soils grazed with cow-calf pairs managed with different stocking rates and densities. The central hypothesis was that irrigated low-density stocking systems (SysB) would result in greater GHG emissions from pasture soils than nonirrigated high-density stocking systems (SysA) and grazing-exclusion (GRE) pasture sites. The nonirrigated high-density stocking systems consisted of 120 cow-calf pairs rotating on a total of 120 ha (stocking rate 1 cow/ha, stocking density 112,000 kg BW/ha, rest period of 60 to 90 d). The irrigated low-density stocking systems consisted of 64 cow-calf pairs rotating on a total of 26 ha of pasture (stocking rate 2.5 cows/ha, stocking density 32,700 kg BW/ha, rest period of 18 to 30 d). Both systems consisted of mixed cool-season grass-legume pastures. Static chambers were randomly placed for collection of CO, CH, and NO samples. Soil temperature (ST), ambient temperature (temperature inside the chamber; AT), and soil water content (WC) were monitored and considered explanatory variables for GHG emissions. GHG fluxes were monitored for 3 yr (2011 to 2013) at the beginning (P1) and at the end (P2) of the grazing season, always postgrazing. Paddock was the experimental unit (3 pseudoreplicates per treatment), and chambers (30 chambers per paddock) were considered multiple measurements of each experimental unit. A completely randomized design considered the term year × period as a repeated measure and chamber nested within paddock and treatment as the random term. Generally, SysB had greater CO emissions than SysA and GRE pasture sites across years and periods ( < 0.01). Soil temperature, AT, and WC had effects on CO emissions. Methane and NO emissions were observed from pasture sites of the 3 systems, but

  6. BESCORP SOIL WASHING SYSTEM FOR LEAD BATTERY SITE TREATMENT - APPLICATIONS ANALYSIS REPORT

    EPA Science Inventory

    This report evaluates the Brice Environmental Services Corporation (BESCORP) Soil Washing System (BSWS) and Its applicability in remediating lead-contaminated soil at lead battery sites. It presents performance and economic data, developed from the U.S. Environmental Protection A...

  7. Nutritional management to optimize fertility of dairy cows in pasture-based systems.

    PubMed

    Butler, S T

    2014-05-01

    The efficiency of milk production in pasture-based systems is heavily influenced by calving pattern, necessitating excellent reproductive performance in a short-breeding season. Where grazed pasture is the major component of the diet, cows are underfed relative to their intake potential. The cow responds by reducing milk output, but fertility is generally better than high intake confinement systems that achieve greater milk production per cow. A number of studies have identified body condition score (BCS) measurements that are related to likelihood of both submission and conception. Blood metabolites and metabolic hormones linked to fertility outcomes are now well characterized. In general, fertility variables have favourable associations with circulating concentrations of glucose, insulin and IGF-1 and unfavourable associations with non-esterified fatty acids, β-hydroxybutyrate and endogenous growth hormone. Nutritional strategies to impact these metabolic indicators have been utilized, but effects on herd fertility are inconsistent. Simply supplementing cows with additional energy in the form of standard concentrates does not appear to have a pronounced effect on fertility. Energy from additional concentrates fed during lactation is preferentially partitioned towards extra milk production rather than BCS repletion. The higher the genetic merit for milk production, the greater the partitioning of additional nutrients to the mammary gland. This review outlines the unique nutritional challenges of pasture-based systems, the role of specific metabolic hormones and metabolites in regulating reproductive function, and nutritional strategies to improve herd fertility.

  8. 33 CFR 157.172 - Limitations on grades of crude oil carried.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.172 Limitations on grades of crude oil carried. If a tank vessel having a COW system meeting § 157.10a(a)(2) or...

  9. Soil intake of lactating dairy cows in intensive strip grazing systems.

    PubMed

    Jurjanz, S; Feidt, C; Pérez-Prieto, L A; Ribeiro Filho, H M N; Rychen, G; Delagarde, R

    2012-08-01

    Involuntary soil intake by cows on pasture can be a potential route of entry for pollutants into the food chain. Therefore, it appears necessary to know and quantify factors affecting soil intake in order to ensure the food safety in outside rearing systems. Thus, soil intake was determined in two Latin square trials with 24 and 12 lactating dairy cows. In Trial 1, the effect of pasture allowance (20 v. 35 kg dry matter (DM) above ground level/cow daily) was studied for two sward types (pure perennial ryegrass v. mixed perennial ryegrass-white clover) in spring. In Trial 2, the effect of pasture allowance (40 v. 65 kg DM above ground level/cow daily) was studied at two supplementation levels (0 or 8 kg DM of a maize silage-based supplement) in autumn. Soil intake was determined by the method based on acid-insoluble ash used as an internal marker. The daily dry soil intake ranged, between treatments, from 0.17 to 0.83 kg per cow in Trial 1 and from 0.15 to 0.85 kg per cow in Trial 2, reaching up to 1.3 kg during some periods. In both trials, soil intake increased with decreasing pasture allowance, by 0.46 and 0.15 kg in Trials 1 and 2, respectively. In Trial 1, this pasture allowance effect was greater on mixed swards than on pure ryegrass swards (0.66 v. 0.26 kg reduction of daily soil intake between medium and low pasture allowance, respectively). In Trial 2, the pasture allowance effect was similar at both supplementation levels. In Trial 2, supplemented cows ate much less soil than unsupplemented cows (0.20 v. 0.75 kg/day, respectively). Differences in soil intake between trials and treatments can be related to grazing conditions, particularly pre-grazing and post-grazing sward height, determining at least in part the time spent grazing close to the ground. A post-grazing sward height lower than 50 mm can be considered as a critical threshold. Finally, a dietary supplement and a low grazing pressure, that is, high pasture allowance increasing post-grazing sward

  10. Performance of Holstein and Swedish-Red × Jersey/Holstein crossbred dairy cows within low- and medium-concentrate grassland-based systems.

    PubMed

    Ferris, C P; Purcell, P J; Gordon, A W; Larsen, T; Vestergaard, M

    2018-05-09

    This 2 × 2 factorial design experiment was conducted to compare the performance of spring-calving Holstein dairy cows (HOL, n = 34) with Swedish Red × Jersey/Holstein crossbred (SR × J/HOL, n = 34) dairy cows within low and medium concentrate input grassland-based dairy systems. The experiment commenced when cows calved and encompassed 1 full lactation. Cows were offered diets containing grass silage and concentrates [70:30 dry matter (DM) ratio, and 40:60 DM ratio, for low and medium, respectively] until turnout, grazed grass plus either 1.0 or 4.0 kg of concentrate/d during the grazing period (low and medium, respectively), and grass silage and concentrates (85:15 DM ratio, and 70:30 DM ratio, for low and medium, respectively) from rehousing and until drying off. No significant genotype × system interactions were present for any of the feed intake or full-lactation milk production data examined. Full-lactation concentrate DM intakes were 769 and 1,902 kg/cow for the low and medium systems, respectively, whereas HOL cows had a higher total DM intake than SR × J/HOL cows in early lactation, but not in late lactation. Although HOL cows had a higher lactation milk yield than SR × J/HOL cows, the latter produced milk with a higher fat and protein content, and thus fat plus protein yield was unaffected by genotype. Milk produced by the SR × J/HOL cows had a higher degree of saturation of fatty acids than milk produced by the HOL cows, and the somatic cell score of milk produced by the former was also higher. Throughout the lactation, HOL cows were on average 30 kg heavier than SR × J/HOL cows, whereas the SR × J/HOL cows had a higher body condition score than the HOL cows. Holstein cows had a higher incidence of mastitis and ovarian dysfunction that SR × J/HOL cows. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  11. Introduction of a closed-system cell processor for red blood cell washing: postimplementation monitoring of safety and efficacy.

    PubMed

    Acker, Jason P; Hansen, Adele L; Yi, Qi-Long; Sondi, Nayana; Cserti-Gazdewich, Christine; Pendergrast, Jacob; Hannach, Barbara

    2016-01-01

    After introduction of a closed-system cell processor, the effect of this product change on safety, efficacy, and utilization of washed red blood cells (RBCs) was assessed. This study was a pre-/postimplementation observational study. Efficacy data were collected from sequentially transfused washed RBCs received as prophylactic therapy by β-thalassemia patients during a 3-month period before and after implementation of the Haemonetics ACP 215 closed-system processor. Before implementation, an open system (TerumoBCT COBE 2991) was used to wash RBCs. The primary endpoint for efficacy was a change in hemoglobin (Hb) concentration corrected for the duration between transfusions. The primary endpoint for safety was the frequency of adverse transfusion reactions (ATRs) in all washed RBCs provided by Canadian Blood Services to the transfusion service for 12 months before and after implementation. Data were analyzed from more than 300 RBCs transfused to 31 recipients before implementation and 29 recipients after implementation. The number of units transfused per episode reduced significantly after implementation, from a mean of 3.5 units to a mean of 3.1 units (p < 0.005). The corrected change in Hb concentration was not significantly different before and after implementation. ATRs occurred in 0.15% of transfusions both before and after implementation. Safety and efficacy of washed RBCs were not affected after introduction of a closed-system cell processor. The ACP 215 allowed for an extended expiry time, improving inventory management and overall utilization of washed RBCs. Transfusion of fewer RBCs per episode reduced exposure of recipients to allogeneic blood products while maintaining efficacy. © 2015 AABB.

  12. 33 CFR 157.166 - Hydrocarbon emissions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.166 Hydrocarbon emissions. If the tank vessel having a COW system under § 157.10a(a)(2) or § 157.10c(b)(2) transfers cargo at a port in...

  13. 33 CFR 157.168 - Crew member: Main deck watch.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.168 Crew member: Main... designated responsibility for monitoring COW operations is on the main deck at all times. ...

  14. 33 CFR 157.168 - Crew member: Main deck watch.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.168 Crew member: Main... designated responsibility for monitoring COW operations is on the main deck at all times. ...

  15. 33 CFR 157.168 - Crew member: Main deck watch.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.168 Crew member: Main... designated responsibility for monitoring COW operations is on the main deck at all times. ...

  16. 33 CFR 157.168 - Crew member: Main deck watch.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.168 Crew member: Main... designated responsibility for monitoring COW operations is on the main deck at all times. ...

  17. 33 CFR 157.126 - Pumps.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.126 Pumps. (a) Crude oil must be supplied to the COW machines by COW system pumps or cargo pumps. (b) The pumps under paragraph...) A sufficient pressure and flow is supplied to allow the simultaneous operation of those COW machines...

  18. Wash Solution Bath Life Extension for the Space Shuttle Rocket Motor Aqueous Cleaning System

    NASA Technical Reports Server (NTRS)

    Saunders, Chad; Evans, Kurt; Sagers, Neil

    1999-01-01

    A spray-in-air aqueous cleaning system, which replaced 1,1,1 trichloroethane (TCA) vapor degreasing, is used for critical cleaning of Space Shuttle Redesigned Solid Rocket Motor (RSRM) metal parts. Small-scale testing demonstrated that the alkaline-based wash solution possesses adequate soil loading and cleaning properties. However, full-scale testing exhibited unexpected depletion of some primary components of the wash solution. Specifically, there was a significant decrease in the concentration of sodium metasilicate which forced change-out of the wash solution after eight days. Extension of wash solution bath life was necessary to ease the burden of frequent change-out on manufacturing. A laboratory study supports a depletion mechanism that is initiated by the hydrolysis of sodium tripolyphosphate (STPP) lowering the pH of the solution. The decrease in pH causes polymerization and subsequent precipitation of sodium metasilicate (SM). Further investigation showed that maintaining the pH was the key to preventing the precipitation of the sodium metasilicate. Implementation to the full scale operation demonstrated that periodic additions of potassium hydroxide (KOH) extended the useful bath life to more than four months.

  19. Breadboard wash water renovation system. [using ferric chloride and ion exchange resins to remove soap and dissolved salts

    NASA Technical Reports Server (NTRS)

    1978-01-01

    A total wash water renovation system concept was developed for removing objectionable materials from spacecraft wash water in order to make the water reusable. The breadboard model system described provides for pretreatment with ferric chloride to remove soap by chemical precipitation, carbon adsorption to remove trace dissolved organics, and ion exchange for removal of dissolved salts. The entire system was put into continuous operation and carefully monitored to assess overall efficiency and equipment maintenance problems that could be expected in actual use. In addition, the capacity of the carbon adsorbers and the ion-exchange resin was calculated and taken into consideration in the final evaluation of the system adequacy. The product water produced was well within the Tentative Wash Water Standards with regard to total organic carbon, conductivity, urea content, sodium chloride content, color, odor, and clarity.

  20. Using activity-based monitoring systems to detect dairy cows in oestrus: a field evaluation.

    PubMed

    Dela Rue, B T; Kamphuis, C; Burke, C R; Jago, J G

    2014-03-01

    To assess the use and performance of activity-based oestrus detection systems (ODS) on two commercial dairy farms using a gold standard based on profiles of concentrations of progesterone in milk, artificial insemination (AI) records and pregnancy diagnosis results. Two activity-based ODS were evaluated in mature cows on two large pasture-grazed dairy farms (>500 cows) over the first 3 weeks of AI. Farm 1 (n=286 cows) used a leg-mounted device and cows were drafted automatically based on activity alerts. Decisions regarding AI were then made based on tail-paint and cow history for these cows. Farm 2 (n=345 cows) used a collar-mounted device and activity alerts were used in conjunction with other information, before the farmer manually selected cows for AI. The gold standard to define the timing of oestrus was based on profiles of concentrations of progesterone in milk measured twice-weekly, used in conjunction with AI records and pregnancy diagnosis results. Sensitivity and positive predictive value (PPV) were calculated for the activity-based ODS data only, and then for AI decisions, against the gold standard. Farm 1 had 195 confirmed oestrus events and 209 activity alerts were generated. The sensitivity of the activity-based ODS was 89.2% with a PPV of 83.3%. Using tail-paint and cow history to confirm activity-based alerts 175 cows were inseminated, resulting in a sensitivity of 89.2% and an improved PPV of 99.4%. Farm 2 had 343 confirmed oestrus events, and 726 alerts were generated by the activity-based ODS, giving a sensitivity of 69.7% with a PPV of 32.9%. A total of 386 cows had AI records, giving a sensitivity of 81.3% and PPV of 72.3%. The two activity-based ODS were used differently on-farm; one automatically selecting cows and the other supporting the manual selection of cows in oestrus. Only one achieved a performance level suggested to be acceptable as a stand-alone ODS. Use of additional tools, such as observation of tail paint to confirm activity

  1. 33 CFR 157.106 - Letter of acceptance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Crude Oil Washing (COW) System on Tank Vessels General § 157.106 Letter of acceptance. The Coast Guard...) The plans submitted show that the COW system meets this subpart; or (b) The plans submitted and the scale model under § 157.104 show that the COW system meets this subpart. ...

  2. A deterministic, dynamic systems model of cow-calf production: The effects of breeding replacement heifers before mature cows over a 10-year horizon.

    PubMed

    Shane, D D; Larson, R L; Sanderson, M W; Miesner, M; White, B J

    2017-10-01

    Some cattle production experts believe that cow-calf producers should breed replacement heifers (nulliparous cows) before cows (primiparous and multiparous cows), sometimes referred to as providing a heifer lead time (tHL). Our objective was to model the effects different durations of tHL may have on measures of herd productivity, including the percent of the herd cycling before the end of the first 21 d of the breeding season (%C21), the percent of the herd pregnant at pregnancy diagnosis (%PPD), the distribution of pregnancy by 21-d breeding intervals, the kilograms of calf weaned per cow exposed (KPC), and the replacement percentage (%RH), using a deterministic, dynamic systems model of cow-calf production over a 10-yr horizon. We also wished to examine differences in the effect of tHL related to the primiparous duration of postpartum anestrus (dPPA). The study model examined 6 different dPPA for primiparous cows (60, 70, 80, 90, 100, or 110 d). The multiparous cow duration of postpartum anestrus was set to 60 d. The breeding season length for nulliparous cows was 63 d, as was the breeding season length for primiparous and multiparous cows. Nulliparous cows were modeled with a tHL of 0, 7, 14, 21, 28, 35, or 42 d. Results are reported for the final breeding season of the 10-yr horizon. Increasing tHL resulted in a greater %C21 for the herd and for primiparous cows. Length of tHL had minimal impact on the %PPD unless the dPPA was 80 d or greater. For a dPPA of 110 d, a 0 d tHL resulted in the herd having 88.1 %PPD. When tHL was 21 d, the %PPD increased to 93.0%. The KPC was 161.2 kg when the dPPA was 110 d and tHL was 0 d and improved to 183.2 kg when tHL was increased to 42 d. The %RH did not vary much unless the dPPA was 90 d or greater, but increasing tHL resulted in decreased %RH. Based on the model results, increasing tHL improves the production outcomes included in the analysis, but herds with dPPA of 90 d or greater had the greatest degree of improvement

  3. Differences in Voluntary Cow Traffic between Holstein and Illawarra Breeds of Dairy Cattle in a Pasture-based Automatic Milking System.

    PubMed

    Clark, C E F; Kwinten, N B P; van Gastel, D A J M; Kerrisk, K L; Lyons, N A; Garcia, S C

    2014-04-01

    Automatic milking systems (AMS) rely upon voluntary cow traffic (the voluntary movement of cattle around a farm) for milk harvesting and feed consumption. Previous research on conventional milking systems has shown differences between dairy cow breeds for intake and milk production, however, the ability to manipulate voluntary cow traffic and milking frequency on AMS farms through breed selection is unknown. This study investigated the effect of breed (Holstein Friesian versus Illawarra) on voluntary cow traffic as determined by gate passes at the Camden AMS research farm dairy facility. Daily data on days in milk, milk yield, gate passes and milking frequency for 158 Holstein Friesian cows and 24 Illawarra cows were collated by month for the 2007 and 2008 years. Illawarra cows had 9% more gate passes/day than Holstein cows over the duration of the study; however, the milking frequency and milk yield of both breeds were similar. Gate passes were greatest for both breeds in early lactation and in the winter (June to August) and summer (December to February) seasons. These findings highlight an opportunity to translate increased voluntary cow movement associated with breed selection into increased milking frequencies, milk production and overall pasture-based AMS performance.

  4. Assessment of different bedding systems for lactating cows in freestall housing

    USDA-ARS?s Scientific Manuscript database

    The objective of this study was to compare different bedding systems for lactating cows in freestall housing. Bedding systems included new sand (NS), recycled byproducts of manure separation (organic solids [OS] and recycled sand [RS]), and foam-core mattresses with a shallow layer of OS (MS). The e...

  5. A comparison of the effects of 2 cattle-cooling systems on dairy cows in a desert environment.

    PubMed

    Ortiz, X A; Smith, J F; Bradford, B J; Harner, J P; Oddy, A

    2010-10-01

    An experiment was conducted to investigate the effects of operation time and size of Korral Kool (KK; Korral Kool Inc., Mesa, AZ) systems on core body temperature (CBT) of dairy cows. Two KK systems were compared: a system with 1.29-m-diameter, 3-hp fans spaced 6 m apart (referred to as small) and a system with 1.52-m-diameter, 5-hp fans spaced 8 m apart (referred to as big). Forty-eight multiparous Holstein cows were assigned randomly to 8 pens (4 big, 4 small), and pens were assigned randomly to a sequence of treatments (KK operated for 21 or 24 h/d) in a switchback design. A complementary calorimetric analysis was developed to investigate the cooling area under the KK units of the big and small systems. Twenty-five sensors distributed equally under the KK units measured ambient temperature at 5-min intervals for 2 h. Average ambient temperature was 35.0±0.6°C and relative humidity was 45±8%. There were significant treatment effects on mean CBT: cows on the small 24-h treatment had a lower mean CBT than cows on the small 21-h treatment (39.22 vs. 39.36±0.14°C), and cows on the big 24-h treatment had a lower mean CBT than cows on the big 21-h treatment (38.95 vs. 39.09±0.13°C). A significant treatment by time interaction was observed. The greatest difference between systems occurred at 0100 h; treatment means at this time were 39.05, 39.01, 39.72, and 39.89±0.16°C for the big 24-h, big 21-h, small 24-h, and small 21-h treatments, respectively. At certain times of day, the big system reduced CBT more than the small system. These results show that CBT of multiparous cows decreased when KK system operational time was increased from 21 to 24 h regardless of the size of the KK cooling system used. The calorimetric analysis showed that even though the big system resulted in lower mean ambient temperatures than the small system, the distance between units in the big system should be decreased to reduce the variation in temperature under the big units. Copyright

  6. The effects of two out-wintering pad systems compared with free-stalls on dairy cow hoof and limb health.

    PubMed

    O'Driscoll, Keelin Km; Hanlon, Alison; French, Padraig; Boyle, Laura A

    2009-02-01

    Lameness is one of the most serious health and welfare problems for dairy cows. This study compared hoof health, limb health and locomotion of dairy cows in three over-winter management systems. Treatments were: (1) an indoor free-stall system (FS), (2) outdoors on an uncovered woodchip pad (UP) and (3) outdoors on a covered woodchip pad (CP). Animals were assigned to treatments at drying off, remained on treatment until parturition, then turned out to pasture. Sole lesions were scored on assignment to treatment, at calving, and 6 weeks and 12 weeks post partum. Locomotion and skin lesions were scored on assignment to treatment, and every 2 weeks until parturition. Post-partum locomotion was scored weekly for 13 weeks. More FS cows were affected by limb lesions than CP cows, and these animals had the worst locomotion scores post calving. There was an effect of inspection, and interactive effect of inspection and treatment on sole lesion scores. UP cows had the highest sole lesion scores 12 weeks post partum probably due to softening of the hooves arising from exposure to moisture while on the woodchip pad. However, post partum, UP cows had better locomotion scores, and CP cows tended to have better scores than cows in FS. Both of these treatments were less likely to have a high tracking-up score than cows in FS. It is possible that cows in this treatment were able to exercise more, owing to the secure underfoot surface and lack of barriers, and this promoted limb flexibility. Although woodchip pads provided benefits for limb health and locomotion, the wood chip surface in both outdoor systems did not offer protective benefits to the hooves relative to housing on concrete. However, the provision of shelter on the pad somewhat overcame the problem of high sole lesion scores in the post-partum period.

  7. Sprinklers and shade cool cows and reduce insect-avoidance behavior in pasture-based dairy systems.

    PubMed

    Kendall, P E; Verkerk, G A; Webster, J R; Tucker, C B

    2007-08-01

    The body temperature of dairy cows in pastoral systems during summer reaches a peak during and following the p.m. milking. Shade and sprinklers can be used separately or in combination at the milking parlor to reduce heat load. Farmers anecdotally report that the use of sprinklers reduces irritation from insects that occurs while cows are waiting for milking. Once daily, we assessed the effectiveness of short-term exposure to shade and sprinklers for cooling cows [via respiration rate and body (vaginal) temperature] and reducing insect-avoidance behaviors before the p.m. milking in a pasture-based dairy system. Head position was measured as an indicator of whether cattle were avoiding water from the sprinklers. Forty-eight Holstein-Friesian dairy cows were divided into 12 groups (4 cows per group, n = 3 groups/treatment) and were exposed to 1 of 4 treatments for 90 min before the p.m. milking: 1) shade, 2) sprinklers, 3) shade and sprinklers, or 4) uncooled control. Respiration rate was reduced by 30% with shade alone compared with controls [54 vs. 78 +/- 2.3 ( +/- SED) breaths/min, respectively]. Sprinklers alone (30 +/- 2.3 breaths/min) and the combined effects of shade and sprinklers (24 +/- 2.3 breaths/min) reduced the respiration rate by 60 and 67%, respectively, compared with controls. Shaded cows had lower body temperatures during the 90-min treatment period compared with controls (shade: 38.6 degrees C; shade and sprinklers: 38.6 degrees C; control: 38.9 +/- 0.09 degrees C). The decrease in body temperature of cows under sprinklers was more marked than for shade alone and remained lower for at least 4 h after milking (sprinklers: 38.7 degrees C; shade and sprinklers: 38.6 degrees C; shade: 38.9 degrees C; control: 39.2 +/- 0.10 degrees C). The sprinkler treatment reduced the number of tail flicks (control: 12.6 vs. sprinklers: 6.6 +/- 2.4 flicks/min) and hoof stamps (control: 4.4 vs. sprinkler: 2.2 +/- 0.5 stamps/min). Cows exposed to sprinklers spent more

  8. Differences in Voluntary Cow Traffic between Holstein and Illawarra Breeds of Dairy Cattle in a Pasture-based Automatic Milking System

    PubMed Central

    Clark, C. E. F.; Kwinten, N. B. P.; van Gastel, D. A. J. M.; Kerrisk, K. L.; Lyons, N. A.; Garcia, S. C.

    2014-01-01

    Automatic milking systems (AMS) rely upon voluntary cow traffic (the voluntary movement of cattle around a farm) for milk harvesting and feed consumption. Previous research on conventional milking systems has shown differences between dairy cow breeds for intake and milk production, however, the ability to manipulate voluntary cow traffic and milking frequency on AMS farms through breed selection is unknown. This study investigated the effect of breed (Holstein Friesian versus Illawarra) on voluntary cow traffic as determined by gate passes at the Camden AMS research farm dairy facility. Daily data on days in milk, milk yield, gate passes and milking frequency for 158 Holstein Friesian cows and 24 Illawarra cows were collated by month for the 2007 and 2008 years. Illawarra cows had 9% more gate passes/day than Holstein cows over the duration of the study; however, the milking frequency and milk yield of both breeds were similar. Gate passes were greatest for both breeds in early lactation and in the winter (June to August) and summer (December to February) seasons. These findings highlight an opportunity to translate increased voluntary cow movement associated with breed selection into increased milking frequencies, milk production and overall pasture-based AMS performance. PMID:25049992

  9. Systemic and local bactericidal potentiality in late lactation Holstein-Friesian cows following a combined antibiotics and Enterococcus faecium SF68 dry-cow treatment.

    PubMed

    Tiantong, Attapol; Piamya, Piya; Chen, Shuen-Ei; Liu, Wen-Bor; Chang, Fang-Yu; Lin, Pei-Chi; Nagahata, Hajime; Chang, Chai-Ju

    2015-08-01

    Antibiotic dry-cow treatment contributes a major part to the total use of antibiotics in dairy herds. Enterococcus faecium strain SF68 (SF68) was of human origin but has been authorized in EU as probiotic feed additive. In the present study, one of the front and rear quarters of twelve late lactation Holstein-Friesian cows were infused once with a commercial antibiotic dry-cow formula (antibiotics quarter) on the first milk-stasis day (d 1), when the contrallateral quarters were infused with 5 x 10(8)-CFU SF68 plus half-dose antibiotic dry-cow formula (SF68/antibiotics quarter) meanwhile. Gelatinase level and cellular reactive oxygen species (ROS) production capacity were measured for blood and quarter secretion. The results showed that the count of blood total leukocytes minorly decreased on d 3 only but the microscopic somatic cell count (MSCC) continuously increased up to d 7, especially in SF68/antibiotics quarters. Plasma level of gelatinase A remained similar up to d 7 but gelatinase B was not detectable in plasma throughout the study. The level of gelatinase A in quarter secretion increased up to d 7 but gelatinase B increased even more drastically, especially in SF68/antibiotics quarters. The ROS production capacity of blood leukocytes increased temporarily only on d 3, but that of milk cells continuously increased up to d 7, especially in SF68/antitiotics quarters. Overall, late lactation Holstein-Friesian cows were systemically adaptable to the combined antibiotics and SF68 dry-cow treatment, while the local bactericidal potentiality in mammary gland was actively responsive to additional SF68 intramammary treatment.

  10. 33 CFR 157.122 - Piping, valves, and fittings.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157..., valves, and fittings of each COW system must: (1) Meet 46 CFR part 56; and (2) Be of steel or an equivalent material accepted by the Commandant. (b) The piping of each COW system must be permanently...

  11. Spatially and temporally variable urinary N loads deposited by lactating cows on a grazing system dairy farm.

    PubMed

    Ahmed, Awais; Sohi, Rajneet; Roohi, Rakhshan; Jois, Markandeya; Raedts, Peter; Aarons, Sharon R

    2018-06-01

    Feed nitrogen (N) intakes in Australian grazing systems average 545 g cow -1 day -1 , indicating that urinary N is likely to be the dominant form excreted. Grazing animals spend disproportionate amounts of time in places on dairy farms where N accumulation is likely to occur. We attached to grazing cows sensors that measure urine volume and N concentration, as well as global positioning systems sensors used to monitor the times the cows spent in different places on a farm and the location of urination events. The cows were monitored for up to 72 h in each of two seasons. More urination events and greater urine volumes per event were recorded in spring 2014 (3.1 L) compared with winter 2015 (1.4 L), most likely influenced by environmental conditions and the greater spring rainfall observed. Mean (range) N concentration (0.71%; 0.02 to 1.52%) and N load (12.8 g cow -1 event -1 ; 0.3 to 64.5 g cow -1 event -1 ) did not differ over the two monitoring periods. However, mean (range) daily N load was greater in spring (277 g cow -1 day -1 ; 200 to 346 g cow -1 day -1 ) than in winter (90 g cow -1 day -1 ; 44 to 116 g cow -1 day -1 ) due to the influence of urine volume. Relatively greater time was spent in paddocks overnight (13.3 h) than in paddocks between morning and evening milking (6.4 h), compared with the mean numbers of urinations in these places (6.4 and 3.8 respectively). The mean N load deposited overnight in paddocks (89.6 g cow -1 ) was more than twice that deposited in paddocks during the day (43.8 g cow -1 ), due to the greater N load per event overnight, and was more closely linked to the relative difference in time spent in paddocks than in the number of urination events. These data suggest that routinely holding cows in the same paddocks overnight will lead to high urinary N depositions, increasing the potential for N losses from these places. Further research using this technology is required to acquire farm and environment

  12. Economic impacts of reduced milk production associated with papillomatous digital dermatitis in dairy cows in the USA.

    PubMed

    Losinger, Willard C

    2006-05-01

    The goal of this study was to measure the economic impacts of reduced milk production associated with papillomatous digital dermatitis (PDD) in dairy cows in the USA, and of specific risk factors for PDD, in 1996. The method applied was an economic-welfare analysis of producer and consumer surplus, with the GUM Workbench used to analyse uncertainties in the measurements. Reduced milk production associated with PDD was found to reduce consumer surplus by Dollars 750 million +/- Dollars 580 million, and to increase the economic surplus of producers by Dollars 560 million +/- Dollars 470 million, with a net economic loss of Dollars 190 million +/- Dollars 130 million. An examination of the economic effects of specific epidemiologic risk factors for PDD showed that having dairy cows that were not born on the operation had important economic consequences associated with the disease, as did the type of land to which dairy cows had access during the winter months and the type of flooring on which cows walked. Washing hoof-trimming equipment between cows was an important biosecurity measure that was associated with reduced PDD. The epidemiologic model used also implicated hoof trimmers who trimmed cattle hooves on other operations as having an important economic impact associated with this disease, although this finding may have been erroneous.

  13. Milk production of Jersey and Fleckvieh × Jersey cows in a pasture-based feeding system.

    PubMed

    Goni, Sindisile; Muller, Carel Johan Christiaan; Dube, Bekezela; Dzama, Kennedy

    2015-01-01

    Milk production parameters of purebred Jersey (J) cows and Fleckvieh × Jersey (F × J) cows in a pasture-based feeding system were compared using standard milk recording procedures. Milk, fat and protein production was adjusted to 305 days per lactation and corrected for age at calving. Effects of breed, parity, month and year were estimated for milk, fat and protein yield as well as fat and protein percentage, using the general linear model procedure. Fixed effects identified as affecting milk production parameters significantly were breed, parity and year. F × J cows produced significantly more milk than J cows (6141 ± 102 and 5398 ± 95 kg milk, respectively). Similarly, fat and protein yields were significantly higher in F × J (272 ± 4 and 201 ± 3 kg, respectively) than in Jersey cows (246 ± 3 and 194 ± 2 kg, respectively). Fat and protein percentages only differed slightly in absolute terms being 4.61 ± 0.04% fat in the Jersey compared to 4.47 ± 0.04% fat in the F × J. Protein levels for J and F × J cows were 3.62 ± 0.03 and 3.51 ± 0.03%, respectively. Despite a lower fat percentage, F × J crossbred cows may be more productive than purebred Jersey cows which may be due to heterotic effects.

  14. Thermoregulatory responses of Holstein and Brown Swiss Heat-Stressed dairy cows to two different cooling systems

    NASA Astrophysics Data System (ADS)

    Correa-Calderon, Abelardo; Armstrong, Dennis; Ray, Donald; DeNise, Sue; Enns, Mark; Howison, Christine

    . Thirty-seven Holstein and 26 Brown Swiss dairy cows were used to evaluate the effect of two different cooling systems on physiological and hormonal responses during the summer. A control group of cows had access only to shade (C). A second group was cooled with spray and fans (S/F) and the third group was under an evaporative cooling system called Korral Kool (KK). The maximum temperature humidity index during the trial was from 73 to 85. Rectal temperatures and respiration rates of the C group were higher (P < 0.05) than those of the S/F and KK groups in both Holstein and Brown Swiss cows. Triiodothyronine levels in milk were higher (P < 0.05) in the KK group than in the S/F and C groups, while cortisol levels were lower (P < 0.05) in the C group than in S/F and KK. There was no significant difference in the hormonal response of the two breeds. These results demonstrate that both cooling systems may be used increase the comfort of Holstein and Brown Swiss cows during summer in hot, dry climates.

  15. 33 CFR 157.146 - Similar tank design: Inspections on U.S. tank vessels.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... RELATING TO TANK VESSELS CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Inspections... Officer in Charge, Marine Inspection, of the zone in which the COW system is inspected, for only one of...

  16. 33 CFR 157.132 - Cargo tanks: Hydrocarbon vapor emissions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.132 Cargo tanks: Hydrocarbon vapor emissions. Each tank vessel having a COW system under § 157.10a...

  17. Comparison of the Laboratory Standard Washing Using CIPAC Washing Agent and the Domestic Washing on Three Recommended Types of Long-Lasting Insecticidal Mosquito Nets

    PubMed Central

    Ouattara, Jean Pierre Nabléni; Louwagie, Johanna; Pigeon, Olivier; Spanoghe, Pieter

    2013-01-01

    Background One of the best ways to prevent malaria is the use of insecticide-treated bed nets. Manufacturers pursue easier, safer and more efficient nets. Hence, many studies on the efficacy and wash resistance using World Health Organization standards have been reported. The commonly used detergent is “Savon de Marseille”, because it closely resembles actually used soaps. At the 54th Collaborative International Pesticides Analytical Council (CIPAC) Technical Meeting in 2010, it was suggested to replace it by a standardized “CIPAC washing agent”. The aim of this study was to investigate the difference between a laboratory hand washing simulation using the CIPAC washing agent (method-1) and a domestic washing (method-2) on different bed nets, as well as the effect of the drying process on the release of active ingredient. Methods Interceptor®, Permanet®2.0 and Netprotect® nets were used in three treatments, each repeated 20 times. The first treatment included method-1 washing and indoor drying. The second treatment included method-2 washing and indoor drying. The third treatment used method-2 washing and UV-drying. The residual insecticide contents were determined using gas chromatography. Results The washing procedure and the number of washes have a significant effect on the release of active ingredient. Statistically, the two washing methods have the same effect on removing the active ingredient from the Interceptor® and Permanet®2.0 net, but a significantly different influence on the Netprotect® nets. The drying process has no significant effect on the insecticide. Conclusion Both washing procedures affected the amount of insecticide remaining on nets independently of the impregnation technology. The active ingredient decreases with the number of washing cycles following an exponential or logarithmic model for coated nets. The laboratory hand washing simulation had more impact on the decrease of active ingredient content of the Netprotect® nets

  18. 33 CFR 157.102 - Plans for foreign tank vessels: Submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.102 Plans for foreign tank vessels: Submission. If the owner or operator of a foreign tank vessel having a COW system under... include— (a) A drawing or diagram of the COW pumping and piping system that meets 46 CFR 56.01-10(d); (b...

  19. 33 CFR 157.118 - Required documents: Foreign tank vessels.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.118 Required documents... having a COW system under § 157.10c(b)(2) shall ensure that the vessel does not enter the navigable...) Evidence that the COW system passed the required inspections by— (i) A document from an authorized CS or...

  20. 33 CFR 157.116 - Required documents: U.S. tank vessels.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.116 Required documents: U.S. tank vessels. The owner, operator, and master of a U.S. tank vessel having a COW system under... COW system consisting of— (1) A document from an authorized CS that certifies the vessel meets § 157...

  1. BIOTROL SOIL WASHING SYSTEM FOR TREATMENT OF A WOOD PRESERVING SITE - APPLICATIONS ANALYSIS REPORT

    EPA Science Inventory

    The report analyzes the results of the SITE Program demonstration of BioTrol's Soil Washing System at the MacGillis & Gibbs wood treatment facility in New Brighton, MN. The contaminants of primary interest are pentachlorophenol (penta) and polynuclear aromatic hydrocarbons (PAHs)...

  2. Subclinical ketosis in dairy cows: prevalence and risk factors in grazing production system.

    PubMed

    Garro, C J; Mian, L; Cobos Roldán, M

    2014-10-01

    The purpose of this study was to estimate the prevalence of Subclinical ketosis (SCK) between 4 and 19 days in milk (DIM) in a grazing production system and investigate the importance of potential risk factors for SCK. This cross-sectional study was conducted in dairy cows (n = 107), which had more of two parities. The concentration of β-hydroxybutyric (BHB) in blood was quantified through a hand-held meter. Potential risk factors evaluated were calving interval (CI), milk yield in previous lactation, metritis, dystocia, calf sex (male), parity (≤3 vs. ≥4) and pre-partum body condition score (BCS ≤ 3.5 vs. ≥3.75). Prevalence of SCK was 10.3% (95% CI 4.7-15) between 4 and 19 DIM. Risk factors identified were the occurrence of both metritis and pre-partum BCS ≥ 3.75. Cows with metritis had 4.9 (95% CI 1.17-20.98) times more risk of developing SCK than cows without metritis. And the cows with pre-partum BCS ≥ 3.75 had 5.25 (95% CI 1.32-21.11) times more risk of developing SCK than cows with pre-partum BCS ≤ 3.5. Metritis could induce a lower feed intake and promote the development of SCK. High pre-partum BCS could induce a greater mobilization of body reserves altering liver function and aggravating post-partum NEB. The results are indicative of the expected prevalence of SCK in grazing production system. Factors associated could help to identify cattle at risk of SCK and improve the management of strategies to limit the effects. Journal of Animal Physiology and Animal Nutrition © 2013 Blackwell Verlag GmbH.

  3. Nasal Wash Treatment

    MedlinePlus

    ... Guidelines Wash your hands. Make the nasal wash solution. Do not use tap water for the nasal ... Whichever water you use to make the saline solution, replace container or water at least weekly. To ...

  4. 33 CFR 157.168 - Crew member: Main deck watch.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.168 Crew member: Main deck watch. During COW operations, the master shall ensure that at least one member of the crew with a designated responsibility for monitoring COW operations is on the main deck at all times. ...

  5. Impact of longevity on greenhouse gas emissions and profitability of individual dairy cows analysed with different system boundaries.

    PubMed

    Grandl, F; Furger, M; Kreuzer, M; Zehetmeier, M

    2018-05-29

    Dairy production systems are often criticized as being major emitters of greenhouse gases (GHG). In this context, the extension of the length of the productive life of dairy cows is gaining interest as a potential GHG mitigation option. In the present study, we investigated cow and system GHG emission intensity and profitability based on data from 30 dairy cows of different productive lifetime fed either no or limited amounts of concentrate. Detailed information concerning productivity, feeding and individual enteric methane emissions of the individuals was available from a controlled experiment and herd book databases. A simplified GHG balance was calculated for each animal based on the milk produced at the time of the experiment and for their entire lifetime milk production. For the lifetime production, we also included the emissions arising from potential beef produced by fattening the offspring of the dairy cows. This accounted for the effect that changes in the length of productive life will affect the replacement rate and thus the number of calves that can be used for beef production. Profitability was assessed by calculating revenues and full economic costs for the cows in the data set. Both emission intensity and profitability were most favourable in cows with long productive life, whereas cows that had not finished their first lactation performed particularly unfavourably with regard to their emissions per unit of product and rearing costs were mostly not repaid. Including the potential beef production, GHG emissions in relation to total production of animal protein also decreased with age, but the overall variability was greater, as the individual cow history (lifetime milk yield, twin births, stillbirths, etc.) added further sources of variation. The present results show that increasing the length of productive life of dairy cows is a viable way to reduce the climate impact and to improve profitability of dairy production.

  6. 33 CFR 157.136 - Two-way voice communications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels Design, Equipment, and Installation § 157.136 Two-way voice communications. Each tank vessel having a COW system under § 157.10(e), § 157.10a(a...

  7. Effect of sensor systems for cow management on milk production, somatic cell count, and reproduction.

    PubMed

    Steeneveld, W; Vernooij, J C M; Hogeveen, H

    2015-06-01

    To improve management on dairy herds, sensor systems have been developed that can measure physiological, behavioral, and production indicators on individual cows. It is not known whether using sensor systems also improves measures of health and production in dairy herds. The objective of this study was to investigate the effect of using sensor systems on measures of health and production in dairy herds. Data of 414 Dutch dairy farms with (n=152) and without (n=262) sensor systems were available. For these herds, information on milk production per cow, days to first service, first calving age, and somatic cell count (SCC) was provided for the years 2003 to 2013. Moreover, year of investment in sensor systems was available. For every farm year, we determined whether that year was before or after the year of investment in sensor systems on farms with an automatic milking system (AMS) or a conventional milking system (CMS), or whether it was a year on a farm that never invested in sensor systems. Separate statistical analyses were performed to determine the effect of sensor systems for mastitis detection (color, SCC, electrical conductivity, and lactate dehydrogenase sensors), estrus detection for dairy cows, estrus detection for young stock, and other sensor systems (weighing platform, rumination time sensor, fat and protein sensor, temperature sensor, milk temperature sensor, urea sensor, β-hydroxybutyrate sensor, and other sensor systems). The AMS farms had a higher average SCC (by 12,000 cells/mL) after sensor investment, and CMS farms with a mastitis detection system had a lower average SCC (by 10,000 cells/mL) in the years after sensor investment. Having sensor systems was associated with a higher average production per cow on AMS farms, and with a lower average production per cow on CMS farms in the years after investment. The most likely reason for this lower milk production after investment was that on 96% of CMS farms, the sensor system investment occurred

  8. Sub-clinical mastitis and associated risk factors on lactating cows in the Savannah Region of Nigeria

    PubMed Central

    2012-01-01

    Background Sub-clinical mastitis limits milk production and represents an important barrier to profitable livestock economics worldwide. Milk production from cows in Nigeria is not at optimum levels in view of many factors including sub-clinical mastitis. Results The overall herd-level prevalence rate for SCM was 85.33% (256/300 heads of cows) while the quarter-level prevalence rate of SCM was 43.25% (519/1,200 quarters). The prevalence of SCM was 50.67%, 43.67%, 39.67% and 39.13% for the left fore-quarter, right hind-quarter, left hind-quarter and right fore-quarter, respectively. The Rahaji breed had the highest prevalence of SCM with 65.91% (29/44), while the White Fulani breed had the least with 32.39% (57/176). A total of 32.33% (97/300) had only one mammary quarter affected, 30.33% (91/300) had two quarters affected, 16.00% (48/300) had three quarters affected while 6.67% (20/300) had all the four quarters affected. A total of 53.00% had SCM in multiple quarters (159/300). The risk of SCM decreased significantly among young lactating cows compared to older animals (OR = 0.283; P < 0.001; 95%CI = 0.155; 0.516). The Rahaji breed had significantly higher risk compared with the White Fulani breed (OR = 8.205; P = 0.013; 95% CI = 1.557; 43.226). Improved sanitation (washing hands before milking) will decrease the risk of SCM (OR = 0.173; P = 0.003; 95% CI = 0.054; 0.554). Conclusion SCM is prevalent among lactating cows in the Nigerian Savannah; and this is associated with both animal characteristics (age, breed and individual milk quarters) and milking practices (hand washing).Good knowledge of the environment and careful management of the identified risk factors with improved sanitation should assist farm managers and veterinarians in implementing preventative programmes to reduce the incidence of SCM. PMID:22894639

  9. Wash water waste pretreatment system

    NASA Technical Reports Server (NTRS)

    1977-01-01

    Investigations were completed on wash waters based on each candidate personal cleansing agent. Evaluations of coagulants, antifoam agents, and the effect of promising antifoams on the chemical precipitation were included. Based on these evaluations two candidate soaps as well as their companion antifoam agents were selected for further work. Operating parameters included the effect of soap concentration, ferric chloride concentration, duration of mixing, and pore size of depth filters on the degree of soap removal. The effect of pressure on water flow through filter cartridges and on the rate of decline of water flow was also investigated. The culmination of the program was the recommendation of a pretreatment concept based on chemical precipitation followed by pressure filtration.

  10. Domestic wash water reclamation

    NASA Technical Reports Server (NTRS)

    Hall, J. B., Jr.; Batten, C. E.; Wilkins, J. R.

    1974-01-01

    System consists of filtration unit, reverse-osmosis module, tanks, pumps, plumbing, and various gauges, meters, and valves. After water is used in washing machine or shower, it is collected in holding tank. Water is pumped through series of five particulate filters. Pressure tank supplies processed water to commode water closet.

  11. Lameness Detection in Dairy Cows: Part 1. How to Distinguish between Non-Lame and Lame Cows Based on Differences in Locomotion or Behavior

    PubMed Central

    Van Nuffel, Annelies; Zwertvaegher, Ingrid; Pluym, Liesbet; Van Weyenberg, Stephanie; Thorup, Vivi M.; Pastell, Matti; Sonck, Bart; Saeys, Wouter

    2015-01-01

    Simple Summary Scoring cattle for lameness based on changes in locomotion or behavior is essential for farmers to find and treat their lame animals. This review discusses the normal locomotion of cows in order to define abnormal locomotion due to lameness. It furthermore provides an overview of various relevant visual locomotion scoring systems that are currently being used as well as practical considerations when assessing lameness on a commercial farm. Abstract Due to its detrimental effect on cow welfare, health and production, lameness in dairy cows has received quite a lot of attention in the last few decades—not only in terms of prevention and treatment of lameness but also in terms of detection, as early treatment might decrease the number of severely lame cows in the herds as well as decrease the direct and indirect costs associated with lameness cases. Generally, lame cows are detected by the herdsman, hoof trimmer or veterinarian based on abnormal locomotion, abnormal behavior or the presence of hoof lesions during routine trimming. In the scientific literature, several guidelines are proposed to detect lame cows based on visual interpretation of the locomotion of individual cows (i.e., locomotion scoring systems). Researchers and the industry have focused on automating such observations to support the farmer in finding the lame cows in their herds, but until now, such automated systems have rarely been used in commercial herds. This review starts with the description of normal locomotion of cows in order to define ‘abnormal’ locomotion caused by lameness. Cow locomotion (gait and posture) and behavioral features that change when a cow becomes lame are described and linked to the existing visual scoring systems. In addition, the lack of information of normal cow gait and a clear description of ‘abnormal’ gait are discussed. Finally, the different set-ups used during locomotion scoring and their influence on the resulting locomotion scores are

  12. Reproductive performances of dairy cows in smallholder production system in Selalle, Central Ethiopia.

    PubMed

    Lobago, F; Bekana, M; Gustafsson, H; Kindahl, H

    2006-05-01

    A study was conducted to assess the reproductive performance and factors that influence reproductive efficiency of cows in smallholder dairy farms in Selalle, Central Ethiopia. Three hundred dairy farms (average herd size = 8) were visited and data on reproductive, breeding and management histories were collected and analysed. The overall geometric means for calving to conception interval (n=382) and duration after last calving (n=422) were 187 and 201 days in pregnant and non-pregnant cows, respectively. The least-squares mean calving to conception interval was higher (p < 0.05) in mixed crop-livestock production (MCLP) than in small urban dairy production (SUDP) systems and was lower (p < 0.01) in non-suckling than in suckling cows. District significantly influenced (p < 0.01) the least-squares mean duration after last calving and, among the districts, non-pregnant cows in Wuchale-Jida had the highest values, whereas cows in Sululta and Mulo had the lowest values. The overall average number of services per conception (+/-SD) and the first service conception rate were 1.6 +/- 1.0 (n=382) and 56% (n=456), respectively. The prevalences of abortion, dystocia (assisted parturition), retained fetal membrane, vulval discharge/endometritis and pre-weaning calf mortality were 1.4%, 1.3%, 5.4%, 2.8% and 17.4%, respectively. The present estimates of extended calving to conception interval and duration after last calving indicate poor reproductive performances of cows in Selalle smallholder dairy farms. The pre-weaning calf mortality rate is highly significant. Accordingly, a further detailed investigation is necessary to identify and quantify the specific reproductive disorders and associated interacting factors attributing to such poor performance and to determine the causes and predisposing factors behind such high calf mortality.

  13. Effects of running time of a cattle-cooling system on core body temperature of cows on dairy farms in an arid environment.

    PubMed

    Ortiz, X A; Smith, J F; Bradford, B J; Harner, J P; Oddy, A

    2010-10-01

    Two experiments were conducted on a commercial dairy farm to describe the effects of a reduction in Korral Kool (KK; Korral Kool Inc., Mesa, AZ) system operating time on core body temperature (CBT) of primiparous and multiparous cows. In the first experiment, KK systems were operated for 18, 21, or 24 h/d while CBT of 63 multiparous Holstein dairy cows was monitored. All treatments started at 0600 h, and KK systems were turned off at 0000 h and 0300 h for the 18-h and 21-h treatments, respectively. Animals were housed in 9 pens and assigned randomly to treatment sequences in a 3 × 3 Latin square design. In the second experiment, 21 multiparous and 21 primiparous cows were housed in 6 pens and assigned randomly to treatment sequences (KK operated for 21 or 24 h/d) in a switchback design. All treatments started at 0600 h, and KK systems were turned off at 0300 h for the 21-h treatments. In experiment 1, cows in the 24-h treatment had a lower mean CBT than cows in the 18- and 21-h treatments (38.97, 39.08, and 39.03±0.04°C, respectively). The significant treatment by time interaction showed that the greatest treatment effects occurred at 0600 h; treatment means at this time were 39.43, 39.37, and 38.88±0.18°C for 18-, 21-, and 24-h treatments, respectively. These results demonstrate that a reduction in KK system running time of ≥3 h/d will increase CBT. In experiment 2, a significant parity by treatment interaction was found. Multiparous cows on the 24-h treatment had lower mean CBT than cows on the 21-h treatment (39.23 and 39.45±0.17°C, respectively), but treatment had no effect on mean CBT of primiparous cows (39.50 and 39.63±0.20°C for 21- and 24-h treatments, respectively). A significant treatment by time interaction was observed, with the greatest treatment effects occurring at 0500 h; treatment means at this time were 39.57, 39.23, 39.89, and 39.04±0.24°C for 21-h primiparous, 24-h primiparous, 21-h multiparous, and 24-h multiparous cows

  14. 33 CFR 157.140 - Tank vessel inspections.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Crude Oil Washing (COW) System on Tank Vessels Inspections § 157.140 Tank vessel inspections. (a) Before... having a COW system under § 157.10(e), § 157.10(a)(2), or § 157.10c(b)(2) and each foreign tank vessel...

  15. Mad Cow Disease

    MedlinePlus

    ... Safe Videos for Educators Search English Español Mad Cow Disease KidsHealth / For Teens / Mad Cow Disease What's ... are people to get it? What Is Mad Cow Disease? Mad cow disease is an incurable, fatal ...

  16. Validation of acid washes as critical control points in hazard analysis and critical control point systems.

    PubMed

    Dormedy, E S; Brashears, M M; Cutter, C N; Burson, D E

    2000-12-01

    A 2% lactic acid wash used in a large meat-processing facility was validated as an effective critical control point (CCP) in a hazard analysis and critical control point (HACCP) plan. We examined the microbial profiles of beef carcasses before the acid wash, beef carcasses immediately after the acid wash, beef carcasses 24 h after the acid wash, beef subprimal cuts from the acid-washed carcasses, and on ground beef made from acid-washed carcasses. Total mesophilic, psychrotrophic, coliforms, generic Escherichia coli, lactic acid bacteria, pseudomonads, and acid-tolerant microorganisms were enumerated on all samples. The presence of Salmonella spp. was also determined. Acid washing significantly reduced all counts except for pseudomonads that were present at very low numbers before acid washing. All other counts continued to stay significantly lower (P < 0.05) than those on pre-acid-washed carcasses throughout all processing steps. Total bacteria, coliforms, and generic E. coli enumerated on ground beef samples were more than 1 log cycle lower than those reported in the U.S. Department of Agriculture Baseline data. This study suggests that acid washes may be effective CCPs in HACCP plans and can significantly reduce the total number of microorganisms present on the carcass and during further processing.

  17. Evaluation of Membrane Systems for Washing/Deglycerolizing Packed Red Blood Cells

    DTIC Science & Technology

    1987-03-20

    d process should reduce the ss than 400 mOsm/kg H20 minutes. The plasma should be less than of centrifugal cessfully demonstrated the rane...2000 ml of wash solution in 35 to 45 minutes. The plasma hemoglobin (Hb) concentration in the washed RBCs should be less than 150 mg/dl. During...plasmapheresis, and the concentration of blood plasma applications, the membranes retain blood but remove water and paration that must be accomplished

  18. Economic and environmental feasibility of a perennial cow dairy farm.

    PubMed

    Rotz, C A; Zartman, D L; Crandall, K L

    2005-08-01

    More efficient and economical production systems are needed to improve the sustainability of dairy farms. One concept to consider is using perennial cows. Perennial cows are those that maintain a relatively high milk production for >or=2 yr without going through the typical dry period followed by calving. Farm records show that some cows have produced over 20 kg/d after 4 yr of continuous lactation. A farm simulation model was used to evaluate the long-term performance, environmental impact, and economics of a conceptual perennial cow production system on a typical dairy farm in Pennsylvania. Compared with a traditional 100-cow farm with replacement heifers produced on the farm, a perennial herd of 100 cows and purchased replacements provided environmental benefit but sustained a substantial economic loss. However, increasing the perennial herd to 128 cows better utilized the feed produced on the farm. Compared with the traditional 100-cow farm, use of the perennial 128-cow herd reduced supplemental protein and mineral feed purchases by 38%, increased annual milk sales by 21%, reduced nitrogen losses by 17%, maintained a phosphorus balance, and increased annual net return to farm management by 3200 dollars. A traditional 120-cow dairy farm with purchased replacements also used a similar amount of farm-produced feed. Compared with this option, the farm with 128 perennial cows reduced protein and mineral feed purchases by 36%, maintained similar annual milk sales, increased manure production by 7%, reduced N losses by 10%, and increased annual net return by 12,700 dollars. The economic feasibility of the perennial-cow dairy farm was very sensitive to the milk production maintained by the perennial herd and market prices for milk and perennial replacement animals. The analysis was relatively insensitive to the assumed useful life of perennial cows as long as they could be maintained in the herd for at least 3 yr. Thus, a perennial cow production system can improve the

  19. 33 CFR 157.154 - Assistant personnel.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Crude Oil Washing (COW) System on Tank Vessels Personnel § 157.154 Assistant personnel. The owner, operator, and master of a tank vessel having a COW system under § 157.10(e), § 157.10a(a)(2), or § 157.10c(b)(2) shall ensure that each member of the crew that has a designated responsibility during COW...

  20. 33 CFR 157.100 - Plans for U.S. tank vessels: Submission.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CARRYING OIL IN BULK Crude Oil Washing (COW) System on Tank Vessels General § 157.100 Plans for U.S. tank vessels: Submission. (a) Before each U.S. tank vessel having a COW system under § 157.10(e), § 157.10a(a... submit to the Coast Guard plans that include— (1) A drawing or diagram of the COW pumping and piping...

  1. Experimental study of the constituents of space wash water

    NASA Technical Reports Server (NTRS)

    Putnam, D. F.; Colombo, G. V.

    1975-01-01

    This report presents experimental data, obtained under controlled conditions, which quantify the various constituents of human origin that may be expected in space wash water. The experiments were conducted with a simulated crew of two male and two female subjects. The data show that the expected wash water contaminants originating from human secretions are substantially lower than theoretical projections indicated. The data presented are immediately useful and may have considerable impact on the tradeoff comparisons among various unit processes and systems under consideration by NASA for recycling space wash water.

  2. Evidence for Active Westward Tilting of Fortymile Wash, Nye County, Nevada

    NASA Astrophysics Data System (ADS)

    McKague, H. L.; Sims, D. W.; Waiting, D. J.

    2006-12-01

    Fortymile Wash is located east and south of a potential high-level nuclear waste repository at Yucca Mountain, Nevada. Several lines of evidence suggest that this may be an area of active westward tilting associated with the continued development of Crater Flat basin and slip on the Bare Mountain normal fault. Near the southern end of Busted Butte, the incised channel of Fortymile Wash changes trend downgradient from south to south-southwest. Further southward, the incised main channel grades to a divergent distributary channel system that shows evidence of increasingly westward tilt. Viewed in profiles oriented normal to the incised channel and across the Fortymile Wash distributary system, topographic elevation of the western margin of the fan decreases southward, resulting in the elevation of the western margin of Fortymile Basin being as much as 18 m [59 ft] lower than the channel system on the eastern fan margin. Mapping of the surficial deposits within the distributary channel system (Pelletier, et al., 2005; Geophy. Res. Ltr., Vol. 32) may be interpreted to show a westward shift (downslope) of the locus of erosional activity toward the topographically lower western fan margin. Most of the older alluvium (Qa3 {86±40-16 ka}) has been eroded from the eastern portion, while incipient incision into the older alluvium is occurring on the western side of the distributary channel system. The results from level-line benchmark surveys (Gilmore, 1992; USGS OFR 92- 450) from 1915 and 1984 show gradual and systematic elevation changes east of the Bare Mountain fault to just east of Amargosa City, Nevada, where a step-like increase occurs. The level-line surveys are near and along the path of U.S. Highway 95, which traverses the distributary channel system of the Fortymile Wash alluvial fan in the southern portion of the Fortymile Wash basin. These lines of evidence indicate disequilibrium in the channel system that would result from active westward tilting of the

  3. Quality of red blood cells washed using a second wash sequence on an automated cell processor.

    PubMed

    Hansen, Adele L; Turner, Tracey R; Kurach, Jayme D R; Acker, Jason P

    2015-10-01

    Washed red blood cells (RBCs) are indicated for immunoglobulin (Ig)A-deficient recipients when RBCs from IgA-deficient donors are not available. Canadian Blood Services recently began using the automated ACP 215 cell processor (Haemonetics Corporation) for RBC washing, and its suitability to produce IgA-deficient RBCs was investigated. RBCs produced from whole blood donations by the buffy coat (BC) and whole blood filtration (WBF) methods were washed using the ACP 215 or the COBE 2991 cell processors and IgA and total protein levels were assessed. A double-wash procedure using the ACP 215 was developed, tested, and validated by assessing hemolysis, hematocrit, recovery, and other in vitro quality variables in RBCs stored after washing, with and without irradiation. A single wash using the ACP 215 did not meet Canadian Standards Association recommendations for washing with more than 2 L of solution and could not consistently reduce IgA to levels suitable for IgA-deficient recipients (24/26 BC RBCs and 0/9 WBF RBCs had IgA levels < 0.05 mg/dL). Using a second wash sequence, all BC and WBF units were washed with more than 2 L and had levels of IgA of less than 0.05 mg/dL. During 7 days' postwash storage, with and without irradiation, double-washed RBCs met quality control criteria, except for the failure of one RBC unit for inadequate (69%) postwash recovery. Using the ACP 215, a double-wash procedure for the production of components for IgA-deficient recipients from either BC or WBF RBCs was developed and validated. © 2015 AABB.

  4. Effects of soap-water wash on human epidermal penetration.

    PubMed

    Zhu, Hanjiang; Jung, Eui-Chang; Phuong, Christina; Hui, Xiaoying; Maibach, Howard

    2016-08-01

    Skin decontamination is a primary interventional method used to decrease dermal absorption of hazardous contaminants, including chemical warfare agents, pesticides and industrial pollutants. Soap and water wash, the most common and readily available decontamination system, may enhance percutaneous absorption through the "wash-in effect." To understand better the effect of soap-water wash on percutaneous penetration, and provide insight to improving skin decontamination methods, in vitro human epidermal penetration rates of four C(14) -labeled model chemicals (hydroquinone, clonidine, benzoic acid and paraoxon) were assayed using flow-through diffusion cells. Stratum corneum (SC) absorption rates of these chemicals at various hydration levels (0-295% of the dry SC weights) were determined and compared with the results of the epidermal penetration study to clarify the effect of SC hydration on skin permeability. Results showed accelerated penetration curves of benzoic acid and paraoxon after surface wash at 30 min postdosing. Thirty minutes after washing (60 min postdosing), penetration rates of hydroquinone and benzoic acid decreased due to reduced amounts of chemical on the skin surface and in the SC. At the end of the experiment (90 min postdosing), a soap-water wash resulted in lower hydroquinone penetration, greater paraoxon penetration and similar levels of benzoic acid and clonidine penetration compared to penetration levels in the non-wash groups. The observed wash-in effect agrees with the enhancement effect of SC hydration on the SC chemical absorption rate. These results suggest SC hydration derived from surface wash to be one cause of the wash-in effect. Further, the occurrence of a wash-in effect is dependent on chemical identity and elapsed time between exposure and onset of decontamination. By reducing chemical residue quantity on skin surface and in the SC reservoir, the soap-water wash may decrease the total quantity of chemical absorbed in the

  5. Application and evaluation of the washing effect in the collector well using pilot plant with washing device

    NASA Astrophysics Data System (ADS)

    Cho, K. H.; Kim, B. J.; Choi, N. C.; Lee, S. J.; Lee, B. H.

    2012-04-01

    Riverbed/bank filtration (RBF) is a natural process used as a first step in drinking water treatment. RBF systems consist of well fields that draw water from an aquifer that is hydraulically connected to surface waters. The benefits of RBF are multiple and include a reduction of turbidity, total coliform, microbial contaminants natural organic matter, and organic contaminants. Some of the disadvantages of RBF include the difficulty of preventing river water from infiltrating the aquifer in in-stances of severe river contamination, the geochemical reaction of the infiltrate with aquifer materials that may raise the aqueous concentrations of Fe2+, Mn2+, As, NH4+, CH4, Ca2+ and HCO3- , and clogging of the riverbed. For example, has demonstrated that riverbed clogging may decrease the specific capacity of RBF wells (flow reduction in the collector well etc.). The objective of this study is to optimization and evaluation the washing effect on various nozzle type and intervals, soil retention rate in the collector well using pilot plant with washing device for prevention flow reduction in the collector well. The Pilot plant experiments were conducted under various conditions; two kinds nozzle type (spray nozzle of circle type (single - Full Cone, multi - Hollow Cone) and spray nozzle of fan shape type (Veejet)), two different nozzle intervals (200 mm, 400mm) and a various soil retention rate in the collector well (10 ~ 40%). The results of experiment showed that in the nozzle type case, the washing effect of the veeject nozzle was more effective than other (Full Cone, Hollow Cone) nozzle through spray results (range, strength and height). In the nozzle interval conditions, washing effect is 200 mm better than 400 mm through spray distance and soil height. The washing efficiency in the collector well increased on soil retention rate decreased and the nozzle injection pressure increased using washing device

  6. An automated walk-over weighing system as a tool for measuring liveweight change in lactating dairy cows.

    PubMed

    Dickinson, R A; Morton, J M; Beggs, D S; Anderson, G A; Pyman, M F; Mansell, P D; Blackwood, C B

    2013-07-01

    Automated walk-over weighing systems can be used to monitor liveweights of cattle. Minimal literature exists to describe agreement between automated and static scales, and no known studies describe repeatability when used for daily measurements of dairy cows. This study establishes the repeatability of an automated walk-over cattle-weighing system, and agreement with static electronic scales, when used in a commercial dairy herd to weigh lactating cows. Forty-six lactating dairy cows from a seasonal calving, pasture-based dairy herd in southwest Victoria, Australia, were weighed once using a set of static scales and repeatedly using an automated walk-over weighing system at the exit of a rotary dairy. Substantial agreement was observed between the automated and static scales when assessed using Lin's concordance correlation coefficient. Weights measured by the automated walkover scales were within 5% of those measured by the static scales in 96% of weighings. Bland and Altman's 95% limits of agreement were -23.3 to 43.6 kg, a range of 66.9 kg. The 95% repeatability coefficient for automated weighings was 46.3 kg. Removal of a single outlier from the data set increased Lin's concordance coefficient, narrowed Bland and Altman's 95% limits of agreement to a range of 32.5 kg, and reduced the 95% repeatability coefficient to 18.7 kg. Cow misbehavior during walk-over weighing accounted for many of the larger weight discrepancies. The automated walk-over weighing system showed substantial agreement with the static scales when assessed using Lin's concordance correlation coefficient. This contrasted with limited agreement when assessed using Bland and Altman's method, largely due to poor repeatability. This suggests the automated weighing system is inadequate for detecting small liveweight differences in individual cows based on comparisons of single weights. Misbehaviors and other factors can result in the recording of spurious values on walk-over scales. Excluding

  7. Productive, economic and risk assessment of grazing dairy systems with supplemented cows milked once a day.

    PubMed

    Lazzarini, B; Lopez-Villalobos, N; Lyons, N; Hendrikse, L; Baudracco, J

    2018-05-01

    Milking cows once a day (OAD) is a herd management practice that may help to reduce working effort and labour demand in dairy farms. However, a decrease in milk yield per cow occurs in OAD systems compared with twice a day (TAD) systems and this may affect profitability of dairy systems. The objective of this study was to assess productive and economic impact and risk of reducing milking frequency from TAD to OAD for grazing dairy systems, using a whole-farm model. Five scenarios were evaluated by deterministic and stochastic simulations: one scenario under TAD milking (TADAR) and four scenarios under OAD milking. The OAD scenarios assumed that milk yield per cow decreased by 30% (OAD30), 24% (OAD24), 19% (OAD19) and 10% (OAD10), compared with TADAR scenario, based on experimental and commercial farms data. Stocking rate (SR) was increased in all OAD scenarios compared to TADAR and two levels of reduction in labour cost were tested, namely 15% and 30%. Milk and concentrate feeds prices, and pasture and crop yields, were allowed to behave stochastically to account for market and climate variations, respectively, to perform risk analyses. Scenario OAD10 showed similar milk yield per ha compared with TADAR, as the increased SR compensated for the reduction in milk yield per cow. For scenarios OAD30, OAD24 and OAD19 the greater number of cows per ha partially compensated for the reduction of milk yield per cow and milk yield per ha decreased 21%, 15% and 10%, respectively, compared with TADAR. Farm operating profit per ha per year also decreased in all OAD scenarios compared with TADAR, and were US$684, US$161, US$ 303, US$424 and US$598 for TADAR, OAD30, OAD24, OAD19, OAD10, respectively, when labour cost was reduced 15% in OAD scenarios. When labour cost was reduced 30% in OAD scenarios, only OAD10 showed higher profit (US$706) than TADAR. Stochastic simulations showed that exposure to risk would be higher in OAD scenarios compared with TADAR. Results showed that OAD

  8. Invited review: Matching forage systems with cow size and environment for sustainable cow-calf production in the southern region of the United States

    USDA-ARS?s Scientific Manuscript database

    Due to increasing red meat demand from the projected increase in world population coupled with reductions in available grazing lands, there has been increased interest in intensification of cow-calf production. Mature cow size has increased by over 30% since the mid-1970s, increasing cow ME requirem...

  9. Animal models to study the impact of nutrition on the immune system of the transition cow.

    PubMed

    Dänicke, Sven; Meyer, Ulrich; Kersten, Susanne; Frahm, Jana

    2018-02-01

    The immune system is particularly challenged in transition cows as marked physiological changes occur in this period which are driven by late gestation, partus and onset of lactation. As a consequence, the metabolic and nutritional state of the cow also changes significantly with possible implications for the plasticity and flexibility of the immune system. In order to understand how the balance between metabolism, nutritional status and the immune system is maintained under challenging conditions, such as an infection, various animal models can be used which specifically manipulate the nutritional status through various feeding and management strategies. Such models aim at exploring the immunological response to a challenge under largely varying nutritional and metabolic states. As energy balance (EB) is strongly associated both with the metabolic state and with the immunoreactivity of the cows the manipulation of the EB by either influencing energy intake or energy excretion with milk, or by both, offers model opportunities for studying EB effects on the immune system. For example, assigning cows with a higher body condition score (BCS) at least 6 weeks prior to calving to an energy-dense diet exceeding the energy requirement in combination with a decelerated increase in the concentrate feed proportion post partum was shown to be effective in inducing a ketotic metabolic state under ad libitum feeding conditions. Compared to an adequately managed control group this model allows studying immune responses in the transit period and in dependence on dietary interventions. Copyright © 2018 Elsevier Ltd. All rights reserved.

  10. 27 CFR 19.328 - Wash water.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Wash water. 19.328 Section... THE TREASURY LIQUORS DISTILLED SPIRITS PLANTS Production Chemical By-Products § 19.328 Wash water. Water used in washing chemicals to remove spirits therefrom may be run into a wash tank or a distilling...

  11. Solvent wash solution

    DOEpatents

    Neace, J.C.

    1984-03-13

    A process is claimed for removing diluent degradation products from a solvent extraction solution, which has been used to recover uranium and plutonium from spent nuclear fuel. A wash solution and the solvent extraction solution are combined. The wash solution contains (a) water and (b) up to about, and including, 50 vol % of at least one-polar water-miscible organic solvent based on the total volume of the water and the highly-polar organic solvent. The wash solution also preferably contains at least one inorganic salt. The diluent degradation products dissolve in the highly-polar organic solvent and the organic solvent extraction solvent do not dissolve in the highly-polar organic solvent. The highly-polar organic solvent and the extraction solvent are separated.

  12. Solvent wash solution

    DOEpatents

    Neace, James C.

    1986-01-01

    Process for removing diluent degradation products from a solvent extraction solution, which has been used to recover uranium and plutonium from spent nuclear fuel. A wash solution and the solvent extraction solution are combined. The wash solution contains (a) water and (b) up to about, and including, 50 volume percent of at least one-polar water-miscible organic solvent based on the total volume of the water and the highly-polar organic solvent. The wash solution also preferably contains at least one inorganic salt. The diluent degradation products dissolve in the highly-polar organic solvent and the organic solvent extraction solvent do not dissolve in the highly-polar organic solvent. The highly-polar organic solvent and the extraction solvent are separated.

  13. 27 CFR 19.310 - Wash water.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Wash water. 19.310 Section 19.310 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF... Byproducts § 19.310 Wash water. Water used in washing chemicals to remove spirits may be run into a wash tank...

  14. 27 CFR 19.310 - Wash water.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Wash water. 19.310 Section 19.310 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF... Byproducts § 19.310 Wash water. Water used in washing chemicals to remove spirits may be run into a wash tank...

  15. 27 CFR 19.310 - Wash water.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Wash water. 19.310 Section 19.310 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF... Byproducts § 19.310 Wash water. Water used in washing chemicals to remove spirits may be run into a wash tank...

  16. 27 CFR 19.310 - Wash water.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Wash water. 19.310 Section 19.310 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF... Byproducts § 19.310 Wash water. Water used in washing chemicals to remove spirits may be run into a wash tank...

  17. Associations of dairy cow behavior, barn hygiene, cow hygiene, and risk of elevated somatic cell count.

    PubMed

    Devries, T J; Aarnoudse, M G; Barkema, H W; Leslie, K E; von Keyserlingk, M A G

    2012-10-01

    Poor dairy cow hygiene has been consistently associated with elevated somatic cell count (SCC) and the risk of subclinical mastitis. The objective of this study was to determine the associations between dairy cow standing and lying behavior, barn hygiene, cow hygiene, and the risk of experiencing elevated SCC. Lactating Holstein dairy cows (n=69; 86 ± 51 DIM; parity: 2.0 ± 1.2; means ± SD), kept in 1 of 2 groups, were monitored over a 4-mo period. Each group contained 61 ± 1 (mean ± SD) cows over the study period; complete data were obtained from 37 and 32 animals within each respective group. Cows were housed in a sand-bedded, freestall barn with 2 symmetrical pens, each with a free cow traffic automatic milking system. To vary barn hygiene, in 4 consecutive 28-d periods, alley manure scrapers in each of the 2 pens were randomly assigned to frequencies of operation of 3, 6, 12, and 24 times per day. During the last 7 d of each period, cow hygiene (upper leg/flank, lower legs, and udder; scale of 1 = very clean to 4 = very dirty) and stall hygiene (number of 0.15×0.15-m squares contaminated with manure in a 1.20×1.65-m grid) were recorded. Standing and lying behavior of the cows were collected during those days using data loggers. Individual-cow SCC was recorded at the beginning and end of each 28-d period. Elevated SCC was used as an indicator of subclinical mastitis; incidence of elevated SCC was defined as having a SCC >200,000 cells/mL at the end of each 28-d period, when SCC was <100,000 cells/mL at the beginning of the period. Less frequent scraping of the barn alleys was associated with cows having poorer hygiene. Poor udder hygiene was associated with poor stall hygiene. Longer lying duration was associated with poor hygiene of the upper legs/flank and udder. Greater premilking standing duration was associated with poor udder hygiene and decreased frequency of lying bouts was associated with poor hygiene of the lower legs. Higher milk yield was

  18. The influence of genetic selection and feed system on the reproductive performance of spring-calving dairy cows within future pasture-based production systems.

    PubMed

    Coleman, J; Pierce, K M; Berry, D P; Brennan, A; Horan, B

    2009-10-01

    Three genetic groups of Holstein-Friesian dairy cows were established from within the Moorepark (Teagasc, Ireland) dairy research herd: LowNA, indicative of the Irish national average-genetic-merit North American Holstein-Friesian; HighNA, high-genetic-merit North American Holstein-Friesian; HighNZ, high-genetic-merit New Zealand Holstein-Friesian. Genetic merit in this study was based on the Irish total merit index, the Economic Breeding Index. Animals from within each genetic group were randomly allocated to 1 of 2 possible post-European Union-milk-quota pasture-based feeding systems (FS): 1) The Moorepark (MP) pasture system (2.64 cows/ha and 500 kg of concentrate supplement per cow per lactation) and 2) a high output per hectare (HC) pasture system (2.85 cows/ha and 1,200 kg of concentrate supplement per cow per lactation). A total of 126, 128, and 140 spring-calving dairy cows were used during the years 2006, 2007, and 2008, respectively. Each group had an individual farmlet of 17 paddocks, and all groups were managed similarly throughout the study. The effects of genetic group, FS, and the interaction between genetic group and FS on reproductive performance, body weight, body condition score, and blood metabolite concentrations were studied using mixed models with factorial arrangements of genetic groups and FS. Odds ratios were used in the analysis of binary fertility traits, and survival analysis was used in the analysis of survival after first calving. When treatment means were compared, the HighNA and HighNZ genotypes (with greater genetic merit for fertility performance) had greater first-service pregnancy rates and had a greater proportion of cows pregnant after 42 d of the breeding season than the LowNA group. Both HighNA and HighNZ genotypes were submitted for artificial insemination earlier in the breeding season and had greater survival than the LowNA genotype. There was no significant FS or genotype by FS interactions for any of the reproductive

  19. Car wash wastewater treatment and water reuse - a case study.

    PubMed

    Zaneti, R N; Etchepare, R; Rubio, J

    2013-01-01

    Recent features of a car wash wastewater reclamation system and results from a full-scale car wash wastewater treatment and recycling process are reported. This upcoming technology comprises a new flocculation-column flotation process, sand filtration, and a final chlorination. A water usage and savings audit (22 weeks) showed that almost 70% reclamation was possible, and fewer than 40 L of fresh water per wash were needed. Wastewater and reclaimed water were characterized by monitoring chemical, physicochemical and biological parameters. Results were discussed in terms of aesthetic quality (water clarification and odour), health (pathological) and chemical (corrosion and scaling) risks. A microbiological risk model was applied and the Escherichia coli proposed criterion for car wash reclaimed water is 200 CFU 100 mL(-1). It is believed that the discussions on car wash wastewater reclamation criteria may assist institutions to create laws in Brazil and elsewhere.

  20. Washing machine related injuries in children: a continuing threat

    PubMed Central

    Warner, B; Kenney, B; Rice, M

    2003-01-01

    Objective: To describe washing machine related injuries in children in the United States. Methods: Injury data for 496 washing machine related injuries documented by the Consumer Product Safety Commission's National Electronic Injury Surveillance System and death certificate data files were analyzed. Gender, age, diagnosis, body part injured, disposition, location and mechanism of injury were considered in the analysis of data. Results: The upper extremities were most frequently injured in washing machine related injuries, especially with wringer machines. Fewer than 10% of patients required admission, but automatic washers accounted for most of these and for both of the deaths. Automatic washer injuries involved a wider range of injury mechanism, including 23 children who fell from the machines while in baby seats. Conclusions: Though most injuries associated with washing machines are minor, some are severe and devastating. Many of the injuries could be avoided with improvements in machine design while others suggest a need for increased education of potential dangers and better supervision of children if they are allowed access to areas where washing machines are operating. Furthermore, washing machines should only be used for their intended purpose. Given the limitations of educational efforts to prevent injuries, health professionals should have a major role in public education regarding these seemingly benign household appliances. PMID:14693900

  1. Effect of complementation of cattle cooling systems with feedline soakers on lactating dairy cows in a desert environment.

    PubMed

    Ortiz, X A; Smith, J F; Bradford, B J; Harner, J P; Oddy, A

    2011-02-01

    Two experiments were conducted on a commercial dairy farm in eastern Saudi Arabia to investigate the effects of Korral Kool (KK; Korral Kool Inc., Mesa, AZ) cattle cooling systems complemented with feedline soakers on core body temperature (CBT) of dairy cows. In both experiments, cows had access to KK 24h/d. In the first experiment, 7 primiparous and 6 multiparous lactating Holstein dairy cows were assigned to 1 of 2 pens, which were assigned randomly to treatment sequence over 4 d in a switchback design. Soakers were on (ON24) or off (OFF24) for 24h/d. For the second experiment, 20 multiparous lactating Holstein cows were assigned randomly to 1 of 2 pens, which were assigned randomly to treatment sequence in a switchback design. This experiment lasted 4 d and feedline soakers alternately remained off or were on (ON12) for 12h/d. In experiment 1, average ambient temperature was 30 ± 0.9°C and average relative humidity was 44 ± 14% (mean ± SD). Feedline soakers complementing KK systems for 24 h/d decreased the mean CBT of lactating dairy cows compared with KK systems alone (38.80 vs. 38.98 ± 0.061°C, respectively). A significant treatment by time interaction was found. The greatest treatment effects occurred at 2100 h; treatment means at this time were 39.26 and 38.85 ± 0.085°C for OFF24 and ON24 treatments, respectively. In experiment 2, average ambient temperature was 35 ± 1.5°C and average relative humidity was 33 ± 16%. Feedline soakers running for 12 h/d significantly decreased the mean 24-h CBT from 39.16 to 38.99 ± 0.084°C. Treatment by time interaction was also significant; the greatest treatment effects occurred at 1500 h, when ON12 reduced CBT from 39.38 to 38.81 ± 0.088°C. These results demonstrate that complementing the KK system with feedline soakers decreased the CBT of dairy cows housed in desert environments. However, the combined systems were not sufficient to lower CBT to normal temperatures in this extreme environment. Copyright

  2. Reproductive performance of lactating dairy cows managed for first service using timed artificial insemination with or without detection of estrus using an activity-monitoring system.

    PubMed

    Fricke, P M; Giordano, J O; Valenza, A; Lopes, G; Amundson, M C; Carvalho, P D

    2014-05-01

    Lactating dairy cows (n=1,025) on a commercial dairy farm were randomly assigned at 10 ± 3 d in milk (DIM) to 1 of 3 treatments for submitting cows to first artificial insemination (AI) and were fitted with activity-monitoring tags (Heatime; SCR Engineers Ltd., Netanya, Israel) at 24 ± 3 DIM. Cows (n=339) in treatment 1 were inseminated based on increased activity from the end of the voluntary waiting period (50 DIM) until submission to an Ovsynch protocol; cows without increased activity from 21 to 65 DIM began an Ovsynch protocol at 65 ± 3 DIM, whereas cows without increased activity from 21 to 50 DIM but not from 51 to 79 DIM began an Ovsynch protocol at 79 ± 3 DIM. Cows (n=340) in treatment 2 were inseminated based on activity after the second PGF2α injection of a Presynch-Ovsynch protocol at 50 DIM, and cows without increased activity began an Ovsynch protocol at 65 ± 3 DIM. Cows (n=346) in treatment 3 were monitored for activity after the second PGF2α injection of a Presynch-Ovsynch protocol, but all cows received timed AI (TAI) at 75 ± 3 DIM after completing the Presynch-Ovsynch protocol. The activity-monitoring system detected increased activity in 56, 69, and 70% of cows in treatments 1, 2, and 3, respectively. Treatment-2 cows had the fewest average days to first AI (62.5), treatment-3 cows had the most average days to first AI (74.9), and treatment-1 cows had intermediate average days to first AI (67.4). Treatment-1 and -2 cows in which inseminations occurred as a combination between increased activity and TAI had fewer overall pregnancies per AI (P/AI) 35 d after AI (32% for both treatments) compared with treatment-3 cows, all of which received TAI after completing the Presynch-Ovsynch protocol (40%). Based on survival analysis, although the rate at which cows were inseminated differed among treatments, treatment did not affect the proportion of cows pregnant by 300 DIM. Thus, use of an activity-monitoring system to inseminate cows based on

  3. TECHNOLOGY EVALUATION REPORT: BIOTROL SOIL WASHING SYSTEM FOR TREATMENT OF A WOOD PRESERVING SITE - VOLUME II

    EPA Science Inventory

    The SITE Program demonstration of one configuration of the BioTrol Soil Washing System (BSWS) was conducted to obtain reliable performance and cost data that can be used to evaluate the potential applicability of the technology as a remediation alternative for sites contaminated ...

  4. Housing system, milk production, and zero-grazing effects on lameness and leg injury in dairy cows.

    PubMed

    Haskell, M J; Rennie, L J; Bowell, V A; Bell, M J; Lawrence, A B

    2006-11-01

    The aim of this study was to assess the effect of grazing (G) vs. zero-grazing (ZG), level of milk production, and quality and type of housing system [free stalls (FS) and straw yards (SY)] on the prevalence of lameness and leg injuries in dairy cows. Observations were made on 37 commercial dairy farms across Great Britain. A single visit of 5 d duration was made to each farm. During this visit, lameness scores and the incidence of swellings, rubs, and injuries to hocks and knees were recorded on all the peak- or mid-lactation cows. Aspects of the quality of housing and management that were likely to affect foot and leg health were recorded. There were more lame cows on ZG farms (39 +/- 0.02%) than on grazing (G) farms (15 +/- 0.01%), and lameness scores were higher on FS farms compared with SY farms (0.25 +/- 0.01 vs. 0.05 +/- 0.01). Cows on SY farms had fewer hock and knee injuries compared with FS farms. The frequency of knee swellings was higher on ZG farms (0.31 +/- 0.02) than on G farms (0.15 +/- 0.01). Aspects of the free-stall design affected foot and leg health. The number of hock swellings increased with increasing stall gradient (0.16 +/- 0.01 with no slope vs. 0.39 +/- 0.02 at a 0 to 1.5% slope). There was an interaction between the length of the free-stall lunging space and the hip width of the cow, indicating that the incidence of lameness is generally highest on farms with small free stalls and heavy cows. High levels of milk production did not affect lameness or leg injury. The results indicate that housing cows throughout the year potentially has a detrimental effect on foot and leg health. However, good free-stall design may reduce lameness and leg lesions.

  5. "Washing-out" ionic liquids from polyethylene glycol to form aqueous biphasic systems.

    PubMed

    Tomé, Luciana I N; Pereira, Jorge F B; Rogers, Robin D; Freire, Mara G; Gomes, José R B; Coutinho, João A P

    2014-02-14

    The molecular-level mechanisms behind the formation of aqueous biphasic systems (ABS) composed of ionic liquids (ILs) and polymers are hitherto not completely understood. For the first time, it is herein shown that polymer-IL-based ABS are a result of a "washing-out" phenomenon, and not of a salting-out effect of the IL over the polymer as assumed in the past few years. Novel evidence is herein provided by experimental results combined with molecular dynamics (MD) simulations and density functional theory (DFT) calculations.

  6. Occupational Hydrofluoric Acid Injury from Car and Truck Washing--Washington State, 2001-2013.

    PubMed

    Reeb-Whitaker, Carolyn K; Eckert, Carly M; Anderson, Naomi J; Bonauto, David K

    2015-08-21

    Exposure to hydrofluoric acid (HF) causes corrosive chemical burns and potentially fatal systemic toxicity. Car and truck wash cleaning products, rust removers, and aluminum brighteners often contain HF because it is efficient in breaking down roadway matter. The death of a truck wash worker from ingestion of an HF-based wash product and 48 occupational HF burn cases associated with car and truck washing in Washington State during 2001-2013 are summarized in this report. Among seven hospitalized workers, two required surgery, and all but one worker returned to the job. Among 48 injured workers, job titles were primarily auto detailer, car wash worker, truck wash worker, and truck driver. Because HF exposure can result in potentially severe health outcomes, efforts to identify less hazardous alternatives to HF-based industrial wash products are warranted.

  7. The effect of lactation number, stage, length, and milking frequency on milk yield in Korean Holstein dairy cows using automatic milking system

    PubMed Central

    Vijayakumar, Mayakrishnan; Park, Ji Hoo; Ki, Kwang Seok; Lim, Dong Hyun; Kim, Sang Bum; Park, Seong Min; Jeong, Ha Yeon; Park, Beom Young; Kim, Tae Il

    2017-01-01

    Objective The aim of the current study was to describe the relationship between milk yield and lactation number, stage, length and milking frequency in Korean Holstein dairy cows using an automatic milking system (AMS). Methods The original data set consisted of observations from April to October 2016 of 780 Holstein cows, with a total of 10,751 milkings. Each time a cow was milked by an AMS during the 24 h, the AMS management system recorded identification numbers of the AMS unit, the cow being milking, date and time of the milking, and milk yield (kg) as measured by the milk meters installed on each AMS unit, date and time of the lactation, lactation stage, milking frequency (NoM). Lactation stage is defined as the number of days milking per cows per lactation. Milk yield was calculated per udder quarter in the AMS and was added to 1 record per cow and trait for each milking. Milking frequency was measured the number of milkings per cow per 24 hour. Results From the study results, a significant relationship was found between the milk yield and lactation number (p<0.001), with the maximum milk yield occurring in the third lactation cows. We recorded the highest milk yield, in a greater lactation length period of early stage (55 to 90 days) at a 4× milking frequency/d, and the lowest milk yield was observed in the later stage (>201 days) of cows. Also, milking frequency had a significant influence on milk yield (p<0.001) in Korean Holstein cows using AMS. Conclusion Detailed knowledge of these factors such as lactation number, stage, length, and milking frequency associated with increasing milk yield using AMS will help guide future recommendations to producers for maximizing milk yield in Korean Dairy industries. PMID:28423887

  8. Comparison of two treatment strategies for cows with metritis in high-risk lactating dairy cows.

    PubMed

    Armengol, Ramon; Fraile, Lorenzo

    2015-05-01

    Acute puerperal metritis (APM) and clinical metritis (CM) are uterine diseases frequently diagnosed in dairy cows. These diseases are responsible for important economic loss because of their effect not only on reproductive performance but also on milk production. The objective of this study was to assess the impact of two different treatments for metritis on dairy cows by measuring their reproductive performance in the next gestation. The end points to measure the reproductive performance included the conception rate at the first artificial insemination, the number of days at conception, and the proportion of nonpregnant cows at over 150 days after beginning milk production. The study was carried out in a high production dairy cow farm located in Lleida (northeast Spain). Recordings of 1044 parturitions of 747 Holstein cows were controlled in this farm from 2009 to 2014. Cows were diagnosed as suffering from metritis (APM or CM) if the following parameters were observed: an abnormally enlarged uterus; a fetid, watery, reddish brown uterine discharge with (APM) or without (CM) fever (>39.5 °C); and presence (APM) or absence (CM) of signs of systemic illness (decreased milk production, dullness, or other signs of toxemia) within 21 days postpartum. Afterwards, cows suffering from metritis (APM or CM) were randomly assigned and balanced to two groups: (1) animals receiving parenteral amoxicillin intramuscularly plus intrauterine infusion with oxytetracycline (P + I group) and (2) animals receiving only parenteral amoxicillin intramuscularly (P group). Furthermore, reproductive performance of cows without metritis was used as reference (control group). Metritis was diagnosed in 27.5% of the total parturitions included in the study (288 of 1044). In particular, metritis was diagnosed in 30.5% (118 of 387) and 25.9% (170 of 657) of parturitions from heifers and multiparous cows, respectively. Reproductive performance was not significantly affected by the parity, the

  9. Liver functional genomics in beef cows on grazing systems: novel genes and pathways revealed.

    PubMed

    Laporta, Jimena; Rosa, Guilherme J M; Naya, Hugo; Carriquiry, Mariana

    2014-02-15

    The adaptation of the liver to periods of negative energy balance is largely unknown in beef cattle on grazing systems. We evaluated liver transcriptome throughout gestation and early lactation of purebred and crossbred beef cows [Angus, Hereford, and their F1 crossbreeds (CR)], grazing high or low herbage allowances (HA) of native grasslands (4 and 2.5 kg dry matter/kg body wt annual mean; n = 16) using an Agilent 4 × 44k bovine array. A total of 4,661 transcripts were affected by days [272 ≥ 2.5-fold difference, false discovery rate (FDR) ≤ 0.10] and 47 pathways were altered during winter gestation (-165 to -15 days relative to calving), when cows experienced decreased body condition score, decreased insulin, and increased nonesterified fatty acid concentrations. Gluconeogenesis and fatty acid oxidation pathways were upregulated, while cell growth, DNA replication, and transcription pathways were downregulated (FDR ≤ 0.25). We observed only small changes in the liver transcriptome during early lactation (+15 to +60 days). A total of 225 genes were differentially expressed (47 ≥ 2-fold difference, FDR ≤ 0.10) between HA. The majority of those were related to glucose and pyruvate metabolism and were upregulated in high HA, reflecting their better metabolic status. Two genes were upregulated in CR cows, but 148 transcripts (74 ≥ 2-fold change difference, FDR ≤ 0.10) were affected by the HA and cow genotype interaction. The transcriptional changes observed indicated a complex and previously unrecognized, hepatic adaptive program of grazing beef cows in different nutritional environments. Novel target candidate genes, metabolic pathways, and regulatory mechanisms were reported.

  10. Production and reproduction of Fleckvieh, Brown Swiss, and 2 strains of Holstein-Friesian cows in a pasture-based, seasonal-calving dairy system.

    PubMed

    Piccand, V; Cutullic, E; Meier, S; Schori, F; Kunz, P L; Roche, J R; Thomet, P

    2013-08-01

    The first objective of this study was to compare the productive and reproductive performance of Holstein-Friesian (CH HF), Fleckvieh (CH FV), and Brown Swiss (CH BS) cows of Swiss origin with New Zealand Holstein-Friesian (NZ HF) cows in pasture-based compact-calving systems; NZ HF cows were chosen as the reference population for such grazing systems. The second objective was to analyze the relationships within and between breeds regarding reproductive performance, milk yield, and body condition score (BCS) dynamics. On 15 commercial Swiss farms, NZ HF cows were paired with Swiss cows over 3 yr. Overall, the study involved 259 complete lactations from 134 cows: 131 from 58 NZ HF, 40 from 24 CH HF, 43 from 27 CH FV, and 45 from 25 CH BS cows. All production parameters were affected by cow breed. Milk and energy-corrected milk yield over 270 d of lactation differed by 1,000 kg between the 2 extreme groups; CH HF having the highest yield and CH BS the lowest. The NZ HF cows had the greatest milk fat and protein concentrations over the lactation and exhibited the highest lactation persistency. Body weight differed by 90 kg between extreme groups; NZ HF and CH BS being the lightest and CH HF and CH FV the heaviest. As a result, the 2 HF strains achieved the highest milk production efficiency (270-d energy-corrected milk/body weight(0.75)). Although less efficient at milk production, CH FV had a high 21-d submission rate (86%) and a high conception rate within 2 inseminations (89%), achieving high pregnancy rates within the first 3 and 6 wk of the breeding period (65 and 81%, respectively). Conversely, poorer reproductive performance was recorded for CH HF cows, with NZ HF and CH BS being intermediate. Both BCS at nadir and at 100 d postpartum had a positive effect on the 6-wk pregnancy rate, even when breed was included in the model. The BCS at 100 d of lactation also positively affected first service conception rate. In conclusion, despite their high milk production

  11. 7 CFR 58.429 - Washing machine.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Washing machine. 58.429 Section 58.429 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards....429 Washing machine. When used, the washing machine for cheese cloths and bandages shall be of...

  12. 7 CFR 58.429 - Washing machine.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Washing machine. 58.429 Section 58.429 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards....429 Washing machine. When used, the washing machine for cheese cloths and bandages shall be of...

  13. Evaluation of a Hand Washing Program for 2nd-Graders

    ERIC Educational Resources Information Center

    Tousman, Stuart; Arnold, Dani; Helland, Wealtha; Roth, Ruth; Heshelman, Nannatte; Castaneda, Oralia; Fischer, Emily; O'Neil, Kristen; Bileto, Stephanie

    2007-01-01

    The purpose of this project was to determine if a multiple-week learner-centered hand washing program could improve hand hygiene behaviors of 2nd-graders in a northern Illinois public school system. Volunteers from the Rockford Hand Washing Coalition went into 19 different classrooms for 4 consecutive weeks and taught a learner-centered program.…

  14. Seasonal changes in serum oxidative stress biomarkers in dairy and beef cows in a daytime grazing system.

    PubMed

    Mirzad, Ahmad Nawid; Tada, Takashi; Ano, Hitoshi; Kobayashi, Ikuo; Yamauchi, Takenori; Katamoto, Hiromu

    2018-01-01

    This study aims to evaluate the oxidative stress during hot summer season using serum oxidative stress biomarkers and elucidate the effects of serum antioxidant vitamin levels in dairy and beef cows in a daytime grazing system. Blood samples were collected once a month from eight Holstein Friesian (HF) and 10 Japanese Black (JB) cows from November 2013 to October 2014. Serum values of derivatives of reactive oxygen metabolites (d-ROMs) tended to be higher in March in both breeds and those in HF cows were kept at higher (P<0.001) levels than those in JB cows during the study period. Serum levels of biological antioxidant potential (BAP) in both breeds were maintained at almost the same values during study period. The OSI [(d-ROMs/BAP) × 100] values in both breeds showed similar seasonal changes, i. e. increase from December to March and decrease from March to August or September. In addition, the OSI values in HF cows were kept at higher (P<0.01) levels than those in JB cows during the study period. Serum concentrations of α-tocopherol, β-carotene, blood urea nitrogen and total cholesterol showed similar seasonal changes in both breeds, low in the winter and high from spring to summer, which may be attributed to the pasture grass intake. Opposite changes in OSI values and serum concentrations of α-tocopherol and β-carotene indicated that antioxidant vitamin levels could affect oxidative stress status.

  15. Seasonal changes in serum oxidative stress biomarkers in dairy and beef cows in a daytime grazing system

    PubMed Central

    MIRZAD, Ahmad Nawid; TADA, Takashi; ANO, Hitoshi; KOBAYASHI, Ikuo; YAMAUCHI, Takenori; KATAMOTO, Hiromu

    2017-01-01

    This study aims to evaluate the oxidative stress during hot summer season using serum oxidative stress biomarkers and elucidate the effects of serum antioxidant vitamin levels in dairy and beef cows in a daytime grazing system. Blood samples were collected once a month from eight Holstein Friesian (HF) and 10 Japanese Black (JB) cows from November 2013 to October 2014. Serum values of derivatives of reactive oxygen metabolites (d-ROMs) tended to be higher in March in both breeds and those in HF cows were kept at higher (P<0.001) levels than those in JB cows during the study period. Serum levels of biological antioxidant potential (BAP) in both breeds were maintained at almost the same values during study period. The OSI [(d-ROMs/BAP) × 100] values in both breeds showed similar seasonal changes, i. e. increase from December to March and decrease from March to August or September. In addition, the OSI values in HF cows were kept at higher (P<0.01) levels than those in JB cows during the study period. Serum concentrations of α-tocopherol, β-carotene, blood urea nitrogen and total cholesterol showed similar seasonal changes in both breeds, low in the winter and high from spring to summer, which may be attributed to the pasture grass intake. Opposite changes in OSI values and serum concentrations of α-tocopherol and β-carotene indicated that antioxidant vitamin levels could affect oxidative stress status. PMID:29142148

  16. Environmental Assessment for the Replacement of a Vehicle Wash Rack, Travis Air Force Base, California

    DTIC Science & Technology

    2005-04-01

    meet state and U.S. Air Force (Air Force) environmental requirements for the use of cleaning products and washing systems; comply with Air Force and...and O/WSs • Use environmentally compliant cleaning products and wash systems • Be equipped with a credit card reader or similar device, to document... cleaning products and washing systems, including operating a covered O/WS within design capacity limits • Comply with Air Force and Department of

  17. Salvage of infected craniotomy bone flaps with the wash-in, wash-out indwelling antibiotic irrigation system. Technical note and case series of 12 patients.

    PubMed

    Auguste, Kurtis I; McDermott, Michael W

    2006-10-01

    When complicated by infection, craniotomy bone flaps are commonly removed, discarded, and delayed cranioplasty is performed. This treatment paradigm is costly, carries the risks associated with additional surgery, and may cause cosmetic deformities. The authors present their experience with an indwelling antibiotic irrigation system used for the sterilization and salvage of infected bone flaps as an alternative to their removal and replacement. The authors retrospectively reviewed the medical records for 12 patients with bone flap infections following craniotomy who received treatment with the wash-in, wash-out indwelling antibiotic irrigation system. Infected flaps were removed and scrubbed with povidone-iodine solution and soaked in 1.5% hydrogen peroxide while the wound was debrided. The bone flaps were returned to the skull and the irrigation system was installed. Antibiotic medication was infused through the system for a mean of 5 days. Intravenous antibiotic therapy was continued for 2 weeks and oral antibiotics for 3 months postoperatively. Wound checks were performed at clinic follow-up visits, and there was a mean follow-up period of 13 months. Eleven of the 12 patients who had undergone placement of the bone flap irrigation system experienced complete resolution of the infection. In five patients there was involvement of the nasal sinus cavities, and in four there was a history of radiation treatment. In the one patient whose infection recurred, there was both involvement of the nasal sinuses and a history of extensive radiation treatment. Infected bone flaps can be salvaged, thus avoiding the cost, risk, and possible disfigurement associated with flap removal and delayed cranioplasty. Although prior radiation treatment and involvement of the nasal sinuses may interfere with wound healing and clearance of the infection, these factors should not preclude the use of irrigation with antibiotic agents for bone flap salvage.

  18. Abortion rate during the second half of gestation of cows undergoing surgery in lateral recumbency as compared with cows of a control population.

    PubMed

    Hirsbrunner, G; Knutti, B; Eicher, R

    1999-01-01

    In a retrospective study, we compared the abortion rates of 59 cows in the second half of gestation, admitted to the Clinic for Food Animals and Horses (1993-1996) with a strictly focal problem (head region, udder and teats, locomotor system) requiring surgery in lateral recumbency, to the abortion rate of a control cow population. Before surgery, a uterus relaxant was given in 42 cases, and 17 cows were untreated as to pregnancy. Cows included in the reproduction health program of the Department of Fertility and Reproduction were used as a control population. Data of 1,736 cows pregnant in the second half of gestation per year were available for the same time period (1993-1996). Abortion rate was 5.08% for the cows undergoing surgery in lateral recumbency as compared with 5.18% for the cows of the control population. There was no significant difference between the two populations (P > 0.05). Thus, the risk for abortion in the cows in question is not elevated as compared with the control population.

  19. Washing machine usage in remote aboriginal communities.

    PubMed

    Lloyd, C R

    1998-10-01

    The use of washing machines was investigated in two remote Aboriginal communities in the Anangu Pitjantjatjara homelands. The aim was to look both at machine reliability and to investigate the health aspect of washing clothes. A total of 39 machines were inspected for wear and component reliability every three months over a one-year period. Of these, 10 machines were monitored in detail for water consumption, hours of use and cycles of operation. The machines monitored were Speed Queen model EA2011 (7 kg washing load) commercial units. The field survey results suggested a high rate of operation of the machines with an average of around 1,100 washing cycles per year (range 150 and 2,300 cycles per year). The results were compared with available figures for the average Australian household. A literature survey, to ascertain the health outcomes relating to washing clothes and bedding, confirmed that washing machines are efficient at removal of bacteria from clothes and bedding but suggested that recontamination of clothing after washing often negated the prior removal. High temperature washing (> 60 degrees C) appeared to be advantageous from a health perspective. With regards to larger organisms, while dust mites and body lice transmission between people would probably be decreased by washing clothes, scabies appeared to be mainly transmitted by body contact and thus transmission would be only marginally decreased by the use of washing machines.

  20. Alternative antimicrobial commercial egg washing procedures

    USDA-ARS?s Scientific Manuscript database

    Commercial table eggs are washed prior to packaging. Standard wash procedures use an alkaline pH and warm water. If a cool water method could be developed that would still provide a microbiologically safe egg, the industry may save energy costs associated with water heating. Four wash procedures ...

  1. Alternative Antimicrobial Commercial Egg Washing Procedures.

    PubMed

    Hudson, Lauren K; Harrison, Mark A; Berrang, Mark E; Jones, Deana R

    2016-07-01

    Commercial table eggs are washed prior to packaging. Standard wash procedures use an alkaline pH and warm water. If a cool water method could be developed that would still provide a microbiologically safe egg, the industry may save energy costs associated with water heating. Four wash procedures were evaluated for Salmonella reduction: pH 11 at 48.9°C (industry standard), pH 11 at ambient temperature (∼20°C), pH 6 at 48.9°C, and pH 6 at ambient temperature. Alkaline washes contained potassium hydroxide-based detergent, while pH 6 washes contained approximately 200 ppm of chlorine and a proprietary chlorine stabilizer (T-128). When eggs were inoculated by immersion in a cell suspension of Salmonella Enteritidis and Salmonella Typhimurium, all treatments resulted in a slight and similar reduction of Salmonella numbers (approximately 0.77 log CFU/ml of shell emulsion reduction). When eggs were inoculated by droplet on the shell surface, Salmonella counts were reduced by approximately 5 log CFU when washed with chlorine plus the chlorine stabilizer at both temperatures and with the alkaline wash at the high temperature. The reductions in Salmonella by these treatments were not significantly (P > 0.05) different from each other but were significantly (P < 0.05) more than the reduction observed for the 20°C alkaline treatment and 20°C control water treatments. Ambient temperature acidic washes reduced Salmonella contamination to the same degree as the standard pH 11 warm water wash and may be a viable option to reduce cost, increase shelf life, and slow pathogen growth in and on shell eggs.

  2. Genetic merit for fertility traits in Holstein cows: I. Production characteristics and reproductive efficiency in a pasture-based system.

    PubMed

    Cummins, S B; Lonergan, P; Evans, A C O; Berry, D P; Evans, R D; Butler, S T

    2012-03-01

    The objective of the present study was to characterize the phenotypic performance of cows with similar proportions of Holstein genetics, similar genetic merit for milk production traits, but with good (Fert+) or poor (Fert-) genetic merit for fertility traits. Specifically, we tested the hypothesis that cows with a negative estimated breeding value for calving interval would have superior fertility performance and would have detectable differences in body reserve mobilization and circulating concentrations of metabolic hormones and metabolites compared with cows that had a positive estimated breeding value for calving interval. For the duration of the study, cows were managed identically as a single herd in a typical grass-based, spring-calving production system. A total of 80 lactation records were available from 26 Fert+ and 26 Fert- cows over 2 consecutive years (2008 and 2009). During yr 1, cows were monitored during a 20-wk breeding season to evaluate reproductive performance. Milk production, body condition score (scale 1 to 5), body weight, grass dry matter intake, energy balance, and metabolic hormone and metabolite data were collected during both years. The Fert+ cows had greater daily milk yield (19.5 vs. 18.7 kg/d), shorter interval from calving to conception (85.6 vs. 113.8 d), and fewer services per cow (1.78 vs. 2.83). No difference between groups in grass dry matter intake, energy balance, or body weight was observed. The Fert+ cows maintained greater BCS during mid (2.84 vs. 2.74 units) and late lactation (2.82 vs. 2.73 units). Circulating concentrations of insulin-like growth factor-I were greater throughout the gestation-lactation cycle in Fert+ cows (148.3 vs. 128.2 ng/mL). The Fert+ cows also had greater circulating concentrations of insulin during the first 4 wk of lactation (1.71 vs. 1.24 μIU/mL). Analysis of records from national herd data verified the association between genetic merit for fertility traits and phenotypic reproductive

  3. Prevalence of lameness and leg lesions of lactating dairy cows housed in southern Brazil: Effects of housing systems.

    PubMed

    Costa, Joao H C; Burnett, Tracy A; von Keyserlingk, Marina A G; Hötzel, Maria J

    2018-03-01

    Within the last few decades, the North American and European dairy industries have been collecting information about lameness and leg injury prevalence on dairy farms and have tried to develop solutions to mitigate these ailments. Few published articles report the prevalence of lameness and leg lesions in areas outside of those 2 regions, or how alternative housing systems, such as compost-bedded packs, affect the prevalence of these maladies. The objectives of this study were to compare the prevalence of lameness and leg lesions on confined dairies that used freestall, compost-bedded packs, or a combination of these 2 systems in Brazil. Data were collected in the autumn and winter of 2016 from 50 dairy farms located in Paraná state, including 12 compost-bedded pack dairies (CB), 23 freestall dairies (FS), and 15 freestall dairies that used compost-bedded packs for vulnerable cows (FS+C). A visit to the farm consisted of a management questionnaire, an inspection of the housing areas as well as the milking parlor, and an evaluation of all lactating cows as they exited the parlor for lameness (score 1-5), hygiene (score 0-2), body condition score (score 1-5), and hock and knee lesions (score 0-1). Median 1-way chi-squared test was used to compare production systems. We found no difference between farm types in management practices related to hoof health management or average daily milk production per cow [31 (29-33.9) kg/d; median (quartile 1-3)], percentage of Holstein cattle in the herd [100% (90-100%)], conception rate [35.8% (30.2-38%)], or pregnancy rate [15% (13.7-18%)]. The CB farms were smaller [85 (49.5-146.5) milking cows] than both the FS [270 (178-327.5) milking cows] and FS+C farms [360 (150-541.5) milking cows). The overall prevalence of severe lameness (score 4 and 5) across all farms was 21.2% (15.2-28.5%) but was lower on the CB farms [14.2% (8.45-15.5%)] in comparison to the FS [22.2% (16.8-26.7%)] and the FS+C farms [22.2% (17.4-32.8%)]. Less than

  4. Will Astronauts Wash Clothes on the Way to Mars?

    NASA Technical Reports Server (NTRS)

    Ewert, Michael K.; Jeng, Frank F.

    2015-01-01

    Future human space exploration missions will lengthen to years, and keeping crews clothed without a huge resupply burden is an important consideration for habitation systems. A space laundry system could be the solution; however, the resources it uses must be accounted for and must win out over the reliable practice of simply bringing along enough spare underwear. NASA has conducted trade-off studies through its Logistics Reduction Project to compare current space clothing systems, life extension of that clothing, traditional water-based clothes washing, and other sanitizing techniques. The best clothing system depends on the mission and assumptions but, in general, analysis results indicate that washing clothes on space missions will start to pay off as mission durations approach a year.

  5. Remediation of cadmium-contaminated paddy soils by washing with chemicals: effect of soil washing on cadmium uptake by soybean.

    PubMed

    Maejima, Yuji; Makino, Tomoyuki; Takano, Hiroyuki; Kamiya, Takashi; Sekiya, Naoki; Itou, Tadashi

    2007-03-01

    We conducted a pot experiment to evaluate the effect of soil washing with CaCl(2) on Cd absorption by two soybean cultivars. The results were as follows: (1) Soybean growth was not significantly different in washed and unwashed soils, but the seed Cd concentration for both cultivars decreased significantly, up to 25%, in the washed soils compared with the unwashed soils. (2) In the washed soils, the Cd concentration in the soil solution indicated an obviously lower value from sowing to the flowering stage; however, the change in Cd speciation was not evident in the CaCl(2)-washed soil solution. Consequently, the effect of soil washing using CaCl(2) on Cd-contaminated paddy soils can be expected to continue after a CaCl(2)-washed paddy field is converted to an upland field.

  6. Hand washing for preventing diarrhoea.

    PubMed

    Ejemot, R I; Ehiri, J E; Meremikwu, M M; Critchley, J A

    2008-01-23

    Diarrhoea is a common cause of morbidity and a leading cause of death among children aged less than five years, particularly in low- and middle-income countries. It is transmitted by ingesting contaminated food or drink, by direct person-to-person contact, or from contaminated hands. Hand washing is one of a range of hygiene promotion interventions that can interrupt the transmission of diarrhoea-causing pathogens. To evaluate the effects of interventions to promote hand washing on diarrhoeal episodes in children and adults. In May 2007, we searched the Cochrane Infectious Diseases Group Specialized Register, CENTRAL (The Cochrane Library 2007, Issue 2), MEDLINE, EMBASE, LILACS, PsycINFO, Science Citation Index and Social Science Citation Index, ERIC (1966 to May 2007), SPECTR, Bibliomap, RoRe, The Grey Literature, and reference lists of articles. We also contacted researchers and organizations in the field. Randomized controlled trials, where the unit of randomization is an institution (eg day-care centre), household, or community, that compared interventions to promote hand washing or a hygiene promotion that included hand washing with no intervention to promote hand washing. Two authors independently assessed trial eligibility and methodological quality. Where appropriate, incidence rate ratios (IRR) were pooled using the generic inverse variance method and random-effects model with 95% confidence intervals (CI). Fourteen randomized controlled trials met the inclusion criteria. Eight trials were institution-based, five were community-based, and one was in a high-risk group (AIDS patients). Interventions promoting hand washing resulted in a 29% reduction in diarrhoea episodes in institutions in high-income countries (IRR 0.71, 95% CI 0.60 to 0.84; 7 trials) and a 31% reduction in such episodes in communities in low- or middle-income countries (IRR 0.69, 95% CI 0.55 to 0.87; 5 trials). Hand washing can reduce diarrhoea episodes by about 30%. This significant

  7. Effect of number of cows in estrus and confinement area on estrous behavior of beef cows.

    PubMed

    Floyd, L N; Lents, C A; White, F J; Wettemann, R P

    2009-06-01

    Mature Angus and Angus x Hereford cows were used to determine the effects of confinement area and number of cows in estrus on estrous behavior. During each of 2 replicates, 32 nonpregnant, nonlactating cows were maintained in a drylot (60 x 100 m) or in a pasture (12 ha). Estrous cycles were synchronized with 2 injections of PGF(2alpha) 10 to 14 d apart at the initiation of the experiment. Thereafter, PGF(2alpha) was administered between d 6 and 18 of the estrous cycle so that 1, 2 to 3, 4 to 6, or > or = 7 cows were in estrus at the same time. Concentrations of progesterone were quantified in plasma at treatment to ensure cows had a normal response to PGF(2alpha). Duration of estrus and the number of mounts received during estrus were recorded by HeatWatch. If any part of estrus for a cow occurred at the same time as estrus for another cow, the cow was considered to have concurrent estrus with the other cow. Cows in the drylot had a shorter (P < 0.02; 61.8 +/- 3.1 h) interval to estrus after PGF(2alpha) compared with cows on pasture (72.8 +/- 3.3 h). The interval to estrus was longer (P < 0.07) when cows were treated with PGF(2alpha) on d 10 to 13 (76.7 +/- 3.3 h) of the estrous cycle than when treated on d 6 to 9 (62.3 +/- 4.7 h) or d 14 to 18 (62.9 +/- 3.6 h). Increasing the number of cows concurrently in estrus increased the number of mounts each cow received per estrus (P < 0.001) and the duration of estrus (P < 0.01). When only 1 cow was in estrus, she received 11.0 +/- 6.2 mounts during 11.6 +/- 1.5 h. When > or = 7 cows were in estrus at the same time, each cow received 50.4 +/- 3.2 mounts during 17.3 +/- 0.8 h. Cows in drylot were in estrus longer (P < 0.04; 16.4 +/- 0.8 h) than cows on pasture (14.2 +/- 0.7 h). Duration of the longest interval between mounts received decreased (P < 0.002) as the number of cows in estrus at one time increased (5.3 +/- 0.7 h for 1 estrous cow; 2.6 +/- 0.3 h when > or = 7 cows were in estrus). We conclude that increasing the

  8. 33 CFR 157.10 - Segregated ballast tanks and crude oil washing systems for certain new vessels.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MARINE ENVIRONMENT RELATING TO TANK VESSELS CARRYING OIL IN BULK Design, Equipment, and Installation § 157.10 Segregated ballast tanks and crude oil washing systems for certain new vessels. (a) This...) Each tank vessel under this section of 20,000 DWT or more that carries crude oil must have a crude oil...

  9. Differential response to stocking rates and feeding by two genotypes of Holstein-Friesian cows in a pasture-based automatic milking system.

    PubMed

    Nieman, C C; Steensma, K M; Rowntree, J E; Beede, D K; Utsumi, S A

    2015-12-01

    The throughput of automatic milking systems (AMS) is likely affected by differential traffic behavior and subsequent effects on the milking frequency and milk production of cows. This study investigated the effect of increasing stocking rate and partial mixed ration (PMR) on the milk production, dry matter intake (DMI), feed conversion efficiency (FCE) and use of AMS by two genotypes of Holstein-Friesian cows in mid-lactation. The study lasted 8 weeks and consisted in a factorial arrangement of two genotypes of dairy cattle, United States Holstein (USH) or New Zealand Friesian (NZF), and two pasture-based feeding treatments, a low stocking rate system (2 cows/ha) fed temperate pasture and concentrate, or a high stocking rate system (HSR; 3 cows/ha) fed same pasture and concentrate plus PMR. A total of 28 cows, 14 USH and 14 NZF, were used for comparisons, with 12 cows, six USH and six NZF, also used for tracking of animal movements. Data were analyzed by repeated measure mixed models for a completely randomized design. No differences (P>0.05) in pre- or post-grazing herbage mass, DMI and FCE were detected in response to increases in stocking rate and PMR feeding in HSR. However, there was a significant (P<0.05) grazing treatment×genotype×week interaction on milk production, explained by differential responses of genotypes to changes in herbage mass over time (P<0.001). A reduction (P<0.01) in hours spent on pasture was detected in response to PMR supplementation in HSR; this reduction was greater (P=0.01) for USH than NZF cows (6 v. 2 h, respectively). Regardless of the grazing treatment, USH cows had greater (P=0.02) milking frequency (2.51 v. 2.26±0.08 milkings/day) and greater (P<0.01) milk yield (27.3 v. 16.0±1.2 kg/day), energy-corrected milk (24.8 v. 16.5±1.0 kg/day), DMI (22.1 v. 16.6±0.8 kg/day) and FCE (1.25 v. 1.01±0.06 kg/kg) than NZF cows. There was also a different distribution of milkings/h between genotypes (P<0.001), with patterns of milkings

  10. The effect of dry cow winter management system on feed intake, performance and estimated energy demand.

    PubMed

    O'Driscoll, K; Boyle, L; Hanlon, A; Buckley, F; French, P

    2010-02-01

    This research compared three wood-chip out-wintering pad (OWP; an unsheltered OWP; a sheltered OWP (both with a concrete feed apron); and an unsheltered OWP with silage provided directly on top of the wood-chip bedding (self-feed OWP)) designs and cubicle housing with regard to dairy cow performance during the pre-partum period, and for 8 weeks post partum. Data were compared during 2 years. In Year 1, the unsheltered (space allowance = 12 m2 per cow) and sheltered (6 m2 per cow) OWPs were compared with cubicle housing (n = 49 cows per treatment). In Year 2, all three OWP designs (12 m2 per cow) were compared with cubicle housing (n = 24 cows per treatment, split into two replicates). Animals were dried off and assigned to treatment in the autumn, and remained there until calving in spring. Subsequently, they were managed at pasture during lactation. Outcome measures for analysis during the pre-partum period were feed intake, live weight, body condition score (BCS), heat production and heat loss, and post-partum were live weight, BCS, milk yield and milk composition. In Year 1, all cows had a similar live weight, but both pre-partum and at calving cows on the unsheltered OWP had a lower BCS than cows in cubicles (P < 0.05). However, in Year 2, there were no differences in either live weight or BCS. In Year 1, cows in the unsheltered OWP produced less heat than in cubicles (P < 0.05), but in Year 2, there was no treatment effect. In both years, cows in unsheltered OWPs lost more heat than cows in the sheltered OWP (P < 0.001). Treatment had no effect on milk composition either year. However, in Year 2, cows in the self-feed OWP had higher milk yields than the other treatments (P < 0.05). The lower BCS and heat production values in unsheltered treatments during Year 1 were probably because of higher rainfall and wind-speed values of that year. However, in both years, live weight in all treatments increased pre partum, and BCS did not decrease, indicating that

  11. Mad Cow Disease (For Parents)

    MedlinePlus

    ... Safe Videos for Educators Search English Español Mad Cow Disease KidsHealth / For Parents / Mad Cow Disease What's ... Is Being Done About It Print About Mad Cow Disease Mad cow disease has been in the ...

  12. Inhibition Of Washed Sludge With Sodium Nitrite

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Congdon, J. W.; Lozier, J. S.

    2012-09-25

    This report describes the results of electrochemical tests used to determine the relationship between the concentration of the aggressive anions in washed sludge and the minimum effective inhibitor concentration. Sodium nitrate was added as the inhibitor because of its compatibility with the DWPF process. A minimum of 0.05M nitrite is required to inhibit the washed sludge simulant solution used in this study. When the worst case compositions and safety margins are considered, it is expected that a minimum operating limit of nearly 0.1M nitrite will be specified. The validity of this limit is dependent on the accuracy of the concentrationsmore » and solubility splits previously reported. Sodium nitrite additions to obtain 0.1M nitrite concentrations in washed sludge will necessitate the additional washing of washed precipitate in order to decrease its sodium nitrite inhibitor requirements sufficiently to remain below the sodium limits in the feed to the DWPF. Nitrite will be the controlling anion in "fresh" washed sludge unless the soluble chloride concentration is about ten times higher than predicted by the solubility splits. Inhibition of "aged" washed sludge will not be a problem unless significant chloride dissolution occurs during storage. It will be very important tomonitor the composition of washed sludge during processing and storage.« less

  13. The effect of concentrate allocation on traffic and milk production of pasture-based cows milked by an automatic milking system.

    PubMed

    Lessire, F; Froidmont, E; Shortall, J; Hornick, J L; Dufrasne, I

    2017-11-01

    Increased economic, societal and environmental challenges facing agriculture are leading to a greater focus on effective way to combine grazing and automatic milking systems (AMS). One of the fundamental aspects of robotic milking is cows' traffic to the AMS. Numerous studies have identified feed provided, either as fresh grass or concentrate supplement, as the main incentive for cows to return to the robot. The aim of this study was to determine the effect of concentrate allocation on voluntary cow traffic from pasture to the robot during the grazing period, to highlight the interactions between grazed pasture and concentrate allocation in terms of substitution rate and the subsequent effect on average milk yield and composition. Thus, 29 grazing cows, milked by a mobile robot, were monitored for the grazing period (4 months). They were assigned to two groups: a low concentrate (LC) group (15 cows) and a high concentrate (HC) group (14 cows) receiving 2 and 4 kg concentrate/cow per day, respectively; two allocations per day of fresh pasture were provided at 0700 and 1600 h. The cows had to go through the AMS to receive the fresh pasture allocation. The effect of concentrate level on robot visitation was calculated by summing milkings, refusals and failed milkings/cow per day. The impact on average daily milk yield and composition was also determined. The interaction between lactation number and month was used as an indicator of pasture availability. Concentrate allocation increased significantly robot visitations in HC (3.60±0.07 visitations/cow per day in HC and 3.10±0.07 visitations/cow per day in LC; P<0.001) while milkings/cow per day were similar in both groups (LC: 2.37±0.02/day and HC: 2.39±0.02/day; Ns). The average daily milk yield over the grazing period was enhanced in HC (22.39±0.22 kg/cow per day in HC and 21.33±0.22 kg/cow per day in LC; P<0.001). However the gain in milk due to higher concentrate supply was limited with regards to the amount

  14. Hand washing promotion for preventing diarrhoea

    PubMed Central

    Ejemot-Nwadiaro, Regina I; Ehiri, John E; Arikpo, Dachi; Meremikwu, Martin M; Critchley, Julia A

    2015-01-01

    Background Diarrhoea accounts for 1.8 million deaths in children in low- and middle-income countries (LMICs). One of the identified strategies to prevent diarrhoea is hand washing. Objectives To assess the effects of hand washing promotion interventions on diarrhoeal episodes in children and adults. Search methods We searched the Cochrane Infectious Diseases Group Specialized Register (27 May 2015); CENTRAL (published in the Cochrane Library 2015, Issue 5); MEDLINE (1966 to 27 May 2015); EMBASE (1974 to 27 May 2015); LILACS (1982 to 27 May 2015); PsycINFO (1967 to 27 May 2015); Science Citation Index and Social Science Citation Index (1981 to 27 May 2015); ERIC (1966 to 27 May 2015); SPECTR (2000 to 27 May 2015); Bibliomap (1990 to 27 May 2015); RoRe, The Grey Literature (2002 to 27 May 2015); World Health Organization (WHO) International Clinical Trial Registry Platform (ICTRP), metaRegister of Controlled Trials (mRCT), and reference lists of articles up to 27 May 2015. We also contacted researchers and organizations in the field. Selection criteria Individually randomized controlled trials (RCTs) and cluster-RCTs that compared the effects of hand washing interventions on diarrhoea episodes in children and adults with no intervention. Data collection and analysis Three review authors independently assessed trial eligibility, extracted data, and assessed risk of bias. We stratified the analyses for child day-care centres or schools, community, and hospital-based settings. Where appropriate, incidence rate ratios (IRR) were pooled using the generic inverse variance method and random-effects model with 95% confidence intervals (CIs). We used the GRADE approach to assess the quality of evidence. Main results We included 22 RCTs: 12 trials from child day-care centres or schools in mainly high-income countries (54,006 participants), nine community-based trials in LMICs (15,303 participants), and one hospital-based trial among people with acquired immune deficiency

  15. Ability of dairy cows to ensure pregnancy according to breed and genetic merit for production traits under contrasted pasture-based systems.

    PubMed

    Bedere, N; Disenhaus, C; Ducrocq, V; Leurent-Colette, S; Delaby, L

    2017-04-01

    The present study aimed to assess and measure the effects of breed, genetic merit for production traits, and feeding systems (FS) on the ability of dairy cows to ensure pregnancy through its components (fertilization, embryonic losses, recalving). An experiment was conducted over 9 yr on Normande and Holstein cows assigned to contrasted FS. Diets were based on maize silage in winter and grazing plus concentrate in spring in the high FS group, and on grass silage in winter and grazing with no concentrate during spring in the low FS group. Within breeds, cows were classified into 2 groups with similar estimated breeding values (EBV) for milk solids: cows with high EBV for milk yield were included in a milk group and those with high EBV for fat and protein contents were included in a content group. Holstein cows produced more milk throughout lactation than Normande cows (the differential was greater in the high FS group, +2,294 kg, compared with +1,280 kg in the low FS group) and lost more body condition to nadir (the differential was greater in the high FS group, -1.00 point, compared with -0.80 point in the low FS group). Within breeds, milk solids production was similar between genetic groups. Cows in the high FS group produced more milk (+2,495 kg for Holstein and +1,481 kg for Normande cows) and had a higher body condition score at nadir (+0.40 point for Holstein and +0.60 point for Normande) than cows in the low FS group. Holstein cows had a lower recalving rate than Normande cows (-19 percentage units). We found no effect of genetic group and FS on fertility of Normande cows. However, according to FS, Holstein cows in the content group exhibited different fertility failure patterns. In the low FS group, Holstein cows in the content group had more nonfertilizations or early embryo mortality (+26 percentage units at first and second services) than Holstein cows in the milk group. In the high FS group, Holstein cows in the content group had a higher proportion of

  16. Associations of udder-health indicators with cow factors and with intramammary infection in dairy cows.

    PubMed

    Nyman, A-K; Persson Waller, K; Bennedsgaard, T W; Larsen, T; Emanuelson, U

    2014-09-01

    The objective of this study was to investigate if and how cow factors and intramammary infection (IMI) are associated with 4 different udder-health indicators in dairy cows as a first step in investigating whether the diagnostic performance of these indicators can be improved. The investigated indicators were somatic cell count (SCC), lactate dehydrogenase (LDH), N-acetyl-β-d-glucosaminidase (NAGase), and alkaline phosphatase (AP) measured in milk. In this cross-sectional study, approximately 1,000 cows from 25 dairy herds were sampled for bacteriology (quarter milk samples) during 3 consecutive days: the day before test milking, at the day of test milking, and at the day after test milking. The whole-udder test milking sample was analyzed for milk composition, SCC, LDH, NAGase, and AP. Cow data (parity, breed, milk yield, percentage of milk fat and protein, milk urea concentration, and days in milk from the sampled test milking) were collected from the Swedish milk-recording scheme. Of the sampled cows 485 were considered IMI negative and were used in multivariable mixed-effect linear regression models to investigate associations between cow factors and the udder-health indicators. A second modeling including all cows, both IMI negative and IMI positive (256 cows), was also performed. The results showed that all udder-health indicators were affected by cow factors but that different cow factors were associated with different indicators. Intramammary-infection status was significantly associated with all udder-health indicators except AP. Parity and milk urea concentration were the only cow factors associated with all indicators in all models. The significant cow factors explained 23% of the variation in SCC and >30% of the variation in LDH, NAGase, and AP in IMI-negative cows, showing that LDH, NAGase, and AP are more affected than SCC by cow factors. The IMI status explained 23% of the variation in SCC in the model with all cows but only 7% of the variation in

  17. Room environment influence on eggshell bacterial levels of non-washed and washed eggs from caged and cage-free laying hens

    USDA-ARS?s Scientific Manuscript database

    The bacteria levels of non-washed and washed eggs obtained from caged and cage-free hens housed in either wire slats or shaving-covered pens were determined. On eight days (from 22 to 52 wk), 20 eggs were collected from each pen. Ten eggs/pen were washed with a commercial egg washing solution, whi...

  18. Associations of herd- and cow-level factors, cow lying behavior, and risk of elevated somatic cell count in free-stall housed lactating dairy cows.

    PubMed

    Watters, M E Alexandrea; Meijer, Karin M A; Barkema, Herman W; Leslie, Kenneth E; von Keyserlingk, Marina A G; Devries, Trevor J

    2013-09-01

    Recent evidence suggests that the risk of intramammary infection in dairy cows is related to lying patterns. The objectives of this study were to quantify the standing and lying behavior of dairy cows milked 3×/d, determine the cow- and herd-level factors associated with these behaviors, and relate these findings to the risk of an elevated somatic cell count (SCC). Five commercial free-stall dairy herds in Eastern Ontario, milking 3×/d, were enrolled in a longitudinal study. Forty Holstein-Friesian cows/herd were randomly selected as focal animals based on days in milk (<200 d) and SCC (<100,000 cells/mL). Farms were followed for 4, 5-week periods. Individual-cow SCC was recorded at the beginning of each period and end of the final period. Elevated SCC (eSCC) was used as an indicator of subclinical mastitis. A new incident eSCC was defined as an individual cow that started the period with a SCC <100,000 cells/mL but whose next SCC exceeded 200,000 cells/mL. Lying behavior was recorded 5d after each milk sampling using data loggers. For these 5d, individual milking times and feeding times were also recorded. On d1 of each recording period 2 trained observers scored focal cows for hygiene and lameness. Throughout the course of the study, cows averaged 11.2h/d of lying time, split into 8.6 lying bouts/d that were on average 84.6 min in length. Later lactation cows had longer daily lying times that were split into fewer lying bouts of longer duration than cows earlier in lactation. Lame cows had longer daily lying times and lying bout durations than non-lame cows. Cows with greater milk yield had lower lying times than lower producing cows. Average post-milking standing time across the study herds was 103 min. Manipulation of feed (feed delivery or push-up) by the stockperson, in the hour before milking or shortly thereafter, resulted in the longest post-milking standing times. Over the study period, 48 new eSCC were detected, resulting in a mean herd incidence rate

  19. Evaluation of the use of an on-farm system for bacteriologic culture of milk from cows with low-grade mastitis.

    PubMed

    Neeser, Nicole L; Hueston, William D; Godden, Sandra M; Bey, Russell F

    2006-01-15

    To determine factors associated with implementation and use of an on-farm system for bacteriologic culture of milk from cows with lowgrade mastitis, including information on how producers used the on-farm bacteriologic culture system to guide antimicrobial selection practices and the resulting impact on patterns of antimicrobial use. Retrospective cohort study. Producers of 81 dairy farms. Farms that used an on-farm system for bacteriologic culture of milk from January 2001 to July 2003 were surveyed. Over half of those producers continuing to use the on-farm culture delayed antimicrobial treatment pending results of bacteriologic culture. Most other producers initiated empirical antimicrobial treatment while bacteriologic culture results were pending. Several barriers to the use of an on-farm system were identified. Significant reductions in rates of antimicrobial use were detected when comparing antimicrobial use rates before and during use of the on-farm system. Most producers chose to treat cows with mastitis caused by gram-positive pathogens with antimicrobials, whereas treatment choices for cows with mastitis caused by gram-negative bacteria and in cases in which no growth was detected varied. Readily available results permit antimicrobial selections to be made on the basis of the causative agent of mastitis. Adoption of an on-farm system for bacteriologic culture of milk may result in significant reductions in the percentage of cows treated with antimicrobials. Decreasing antimicrobial use may have several benefits including preventing unnecessary discarding of milk, decreasing the potential for drug residues in milk, and improving treatment outcomes as a result of targeted treatments.

  20. Investigating locomotion of dairy cows by use of high speed cinematography.

    PubMed

    Herlin, A H; Drevemo, S

    1997-05-01

    The longterm influence of management systems on the locomotion of 17 dairy cows was investigated by high speed cinematography (100 frames/s) and kinematic analysis. Angular patterns and hoof trajectories of the left fore- and hindlimbs are presented and statistics made of occurring minimum and maximum angles. At the recording, 3 cows had been kept in tie-stalls (TI) and 6 cows in cubicles (CI) for a consecutive time of about 2.5 years while 8 cows had been kept on grass for about 3 months. Four of the grazing cows had earlier been kept in cubicles (CG) and 4 in tie-stalls (TG) during earlier off grazing seasons together with TI and CI cows. The CI cows had a smaller maximum angle of the elbow joint compared to TI, TG and CG cows. The hock joint angle of the CI cows was less flexed during the stance phase than in TI and CG cows while the minimum angle during the swing phase was greater in the TI and CI cows compared to TG and CG cows. Pastured cows (TG and CG) had a less pronounced flexion of the fetlock joint angle during the stance compared to cows kept indoors (TI and CI). The results suggest that slatted floor and lack of exercise during summer grazing may affect locomotion. This is indicated by restrictions in the movements of the elbow and hock joints and in less fetlock joint flexion at full support.

  1. SITE TECHNOLOGY CAPSULE: BIOGENESIS SOIL WASHING TECHNOLOGY

    EPA Science Inventory

    Soil washing technologies are designed to transfer contaminants from soil to a liquid phase. The BloGenesis™ soil washing technology uses a proprietary surfactant solution to transfer organic contaminants from soil to wastewater. The surfactant used in the soil washing process wa...

  2. Effect of mature body weight and stocking rate on cow and calf performance, cow herd efficiency, and economics in the southeastern United States.

    PubMed

    Beck, P A; Stewart, C B; Gadberry, M S; Haque, M; Biermacher, J

    2016-04-01

    Eight 4-ha mixed warm-season grass pastures in southwestern Arkansas (33°40'4″ N, 93°35'24″ W, and elevation 107 m) were stocked with either large mature size (571 kg [SD 55.2] BW) or small mature size (463 kg [SD 58.2] BW) spring-calving cows at 4 stocking rates (SR; 1, 1.5, 2, or 2.5 cow-calf pairs/ha) over 4 yr to test the effects of SR and mature body size on cow and calf performance and system economics. Each pasture received 112 kg/ha N as ammonium nitrate in May and was broadcast seeded to annual ryegrass ( Lam.) in mid October each fall along with 112 kg/ha N as ammonium nitrate. Data were analyzed by regression to determine the effects of cow size and SR on calf performance, cow BW change, calf gain, weaning weight per hectare, hay feeding requirements, and net returns. As SR increased, cow BW and BCS at weaning decreased ( < 0.01) by 26 kg and 0.36 condition scores, respectively, for each additional cow stocked per hectare ( = 0.44). Calf BW at weaning in October increased ( < 0.01) 19 kg for each 100-kg increase in cow BW but was not affected ( = 0.66) by SR. As cow BW increased, calf BW at weaning per 100 kg cow BW decreased ( < 0.01) 6.7 kg for each 100-kg increase in cow BW but was not affected ( = 0.44) by SR. Neither cow BW nor SR affected ( ≥ 0.53) pregnancy percentage, which averaged 88% over the 4-yr experiment. Calf BW weaned per hectare was not affected ( = 0.75) by cow BW but linearly increased ( < 0.01) by 217 kg for each additional cow per hectare SR. Hay feeding days and cost of hay per cow increased ( ≤ 0.05) and kilograms of hay offered per cow tended ( = 0.09) to linearly increase with increasing SR, yet cow BW had no effects ( > 0.22). Although there were no effects ( ≥ 0.38) of cow BW on carrying cost or net returns, increasing SR decreased ( < 0.01) total expenses by US$102/cow and increased net returns by $70/cow and $438/ha for each cow per hectare increase in SR. These data indicate that increasing cow size can increase

  3. Behavior and milk production of buffalo cows as affected by housing system.

    PubMed

    De Rosa, G; Grasso, F; Braghieri, A; Bilancione, A; Di Francia, A; Napolitano, F

    2009-03-01

    To verify the effect of 2 housing systems (with and without a pool and an ample outdoor lot) on behavior and milk yield, 45 lactating buffalo cows were group-housed in a free stall open-sided barn with concrete floor where they received 10 m(2)/head as space allowance (group NP); 43 cows were group-housed in a similar barn, but had access to an outdoor yard (36 m(2)/head) and a concrete pool of 208 m(2) (group WP). Animals were subjected to 8 sessions of instantaneous scan sampling at approximately 10-d intervals. Behavioral variables were expressed as proportions of subjects observed in each category of posture and activity. In addition, rapid behaviors such as agonistic, social, and reproductive interactions, social licking, and self-grooming were recorded continuously. These variables were expressed as number of interactions per animal. At the end of each hour of observation, temperature and relative humidity were recorded. In WP the proportion of animals observed wallowing was 0.476 +/- 0.034, whereas lower proportions were observed standing (0.389 +/- 0.029) or lying (0.141 +/- 0.021) outside the pool. In NP the proportions of animals observed standing and lying were 0.452 +/- 0.042 and 0.548 +/- 0.042, respectively. A significant relationship between mean temperatures recorded on observation days and proportion of animals in the pool was observed (r(s) = 0.41). Fewer animals from group WP were observed idling compared with buffaloes from group NP (0.44 +/- 0.024 vs. 0.509 +/- 0.024, respectively), whereas more WP animals were involved in investigative activities than NP cows (0.099 +/- 0.009 vs. 0.042 +/- 0.009, respectively). A greater number of social interactions (sniffing and nuzzling) and social lickings were observed in group WP than in group NP (0.120 +/- 0.010 vs. 0.067 +/- 0.010, and 0.151 +/- 0.018 vs. 0.090 +/- 0.018, respectively). The WP buffalo cows had a greater milk yield than NP cows (11.73 +/- 0.31 vs. 10.78 +/- 0.28 kg/d, respectively

  4. 30 CFR 1206.260 - Allocation of washed coal.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 3 2011-07-01 2011-07-01 false Allocation of washed coal. 1206.260 Section... INTERIOR Natural Resources Revenue PRODUCT VALUATION Federal Coal § 1206.260 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was...

  5. 30 CFR 1206.459 - Allocation of washed coal.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 3 2011-07-01 2011-07-01 false Allocation of washed coal. 1206.459 Section... INTERIOR Natural Resources Revenue PRODUCT VALUATION Indian Coal § 1206.459 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was...

  6. Milk drop due to leptospirosis in dairy cows.

    PubMed

    2015-03-07

    Leptospiral milk drop in dairy cows. Pseudomonas aeruginosa mastitis in a cow. Systemic pasteurellosis in lambs. Encephalopathy due to water deprivation/salt poisoning suspected in weaned lambs. Biliary cystadenoma in a red deer hind. These are among matters discussed in the disease surveillance report for November 2014 from SAC Consulting: Veterinary Services (SAC C VS). British Veterinary Association.

  7. Physicochemical Quality and Chemical Safety of Chlorine as a Reconditioning Agent and Wash Water Disinfectant for Fresh-Cut Lettuce Washing

    PubMed Central

    Van Haute, Sam; Holvoet, Kevin; Uyttendaele, Mieke

    2013-01-01

    Chlorine was assessed as a reconditioning agent and wash water disinfectant in the fresh-cut produce industry. Artificial fresh-cut lettuce wash water, made from butterhead lettuce, was used for the experiments. In the reconditioning experiments, chlorine was added to artificial wash water inoculated with Escherichia coli O157 (6 log CFU/ml). Regression models were constructed based on the inactivation data and validated in actual wash water from leafy vegetable processing companies. The model that incorporated chlorine dose and chemical oxygen demand (COD) of the wash water accurately predicted inactivation. Listeria monocytogenes was more resistant to chlorine reconditioning in artificial wash water than Salmonella spp. and Escherichia coli O157. During the washing process with inoculated lettuce (4 log CFU/g), in the absence of chlorine, there was a rapid microbial buildup in the water that accumulated to 5.4 ± 0.4 log CFU/100 ml after 1 h. When maintaining a residual concentration of 1 mg/liter free chlorine, wash water contamination was maintained below 2.7, 2.5, and 2.5 log CFU/100 ml for tap water and artificial process water with COD values of 500 and 1,000 mg O2/liter, respectively. A model was developed to predict water contamination during the dynamic washing process. Only minor amounts of total trihalomethanes were formed in the water during reconditioning. Total trihalomethanes accumulated to larger amounts in the water during the wash water disinfection experiments and reached 124.5 ± 13.4 μg/liter after 1 h of execution of the washing process in water with a COD of 1,000 mg O2/liter. However, no total trihalomethanes were found on the fresh-cut lettuce after rinsing. PMID:23396332

  8. Physicochemical quality and chemical safety of chlorine as a reconditioning agent and wash water disinfectant for fresh-cut lettuce washing.

    PubMed

    Van Haute, Sam; Sampers, Imca; Holvoet, Kevin; Uyttendaele, Mieke

    2013-05-01

    Chlorine was assessed as a reconditioning agent and wash water disinfectant in the fresh-cut produce industry. Artificial fresh-cut lettuce wash water, made from butterhead lettuce, was used for the experiments. In the reconditioning experiments, chlorine was added to artificial wash water inoculated with Escherichia coli O157 (6 log CFU/ml). Regression models were constructed based on the inactivation data and validated in actual wash water from leafy vegetable processing companies. The model that incorporated chlorine dose and chemical oxygen demand (COD) of the wash water accurately predicted inactivation. Listeria monocytogenes was more resistant to chlorine reconditioning in artificial wash water than Salmonella spp. and Escherichia coli O157. During the washing process with inoculated lettuce (4 log CFU/g), in the absence of chlorine, there was a rapid microbial buildup in the water that accumulated to 5.4 ± 0.4 log CFU/100 ml after 1 h. When maintaining a residual concentration of 1 mg/liter free chlorine, wash water contamination was maintained below 2.7, 2.5, and 2.5 log CFU/100 ml for tap water and artificial process water with COD values of 500 and 1,000 mg O2/liter, respectively. A model was developed to predict water contamination during the dynamic washing process. Only minor amounts of total trihalomethanes were formed in the water during reconditioning. Total trihalomethanes accumulated to larger amounts in the water during the wash water disinfection experiments and reached 124.5 ± 13.4 μg/liter after 1 h of execution of the washing process in water with a COD of 1,000 mg O2/liter. However, no total trihalomethanes were found on the fresh-cut lettuce after rinsing.

  9. Winter feeding systems and dairy cow breed have an impact on milk composition and flavour of two Protected Designation of Origin French cheeses.

    PubMed

    Hurtaud, C; Peyraud, J L; Michel, G; Berthelot, D; Delaby, L

    2009-09-01

    This study investigates the effects of two feeding systems and two dairy cow breeds on milk yield and composition, physical and sensorial properties of Camembert and Pont-l'Evêque cheeses. The experiment consisted of a 2 × 2 factorial arrangement of treatments. A low energy grass diet with only 15% of concentrate (LowGS) was compared with a high-energy maize silage diet with 30% concentrate (HighMS). Thirty-four Holstein (Ho) and 34 Normande (No) cows in early lactation were assigned to one of two feeding systems for a 6-week period. Cows on the LowGS feeding system had lower milk yield, fat and protein content. In both feeding systems, No cows had lower milk yields but higher milk protein contents than Ho cows. The LowGS feeding system altered milk fatty acid (FA) composition by reducing saturated FA. Breed had only a small impact on milk FA. Concerning milk coagulating properties, only the firmness was reduced by the LowGS feeding and the Ho breed. The effects of breed and feeding system on the protein content of cheeses were more marked in Camembert cheese than in Pont-l'Evêque cheese. However, the Camembert cheese from Ho-LowGS was, in fact, characterized especially by lower protein content. LowGS feeding system and No breed produced more yellow cheeses. Feeding systems had limited effects on the firmness of Camembert and Pont-l'Evêque cheeses measured by penetrometry. In sensory analysis, Ho breed and LowGS feeding produced a Camembert cheese with a more melting texture in the mouth due to the increase of spreadability index and of proteolysis. The type of cheese also had an influence: the effects were more important on Camembert cheese than on Pont-l'Evêque cheese. Only the Ho-LowGS treatment produced a very specific Camembert cheese different from the others. The feeding systems and breed of dairy cow have no determinant effect on PDO (protected designation of origin) Camembert and Pont-l'Evêque cheeses, especially regarding taste. In this kind of

  10. 30 CFR 1206.459 - Allocation of washed coal.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Allocation of washed coal. 1206.459 Section... RESOURCES REVENUE PRODUCT VALUATION Indian Coal § 1206.459 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was extracted. (b) When...

  11. 30 CFR 1206.459 - Allocation of washed coal.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Allocation of washed coal. 1206.459 Section... RESOURCES REVENUE PRODUCT VALUATION Indian Coal § 1206.459 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was extracted. (b) When...

  12. 30 CFR 1206.260 - Allocation of washed coal.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Allocation of washed coal. 1206.260 Section... RESOURCES REVENUE PRODUCT VALUATION Federal Coal § 1206.260 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was extracted. (b) When...

  13. 30 CFR 1206.260 - Allocation of washed coal.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 3 2012-07-01 2012-07-01 false Allocation of washed coal. 1206.260 Section... RESOURCES REVENUE PRODUCT VALUATION Federal Coal § 1206.260 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was extracted. (b) When...

  14. 30 CFR 1206.459 - Allocation of washed coal.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 3 2014-07-01 2014-07-01 false Allocation of washed coal. 1206.459 Section... RESOURCES REVENUE PRODUCT VALUATION Indian Coal § 1206.459 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was extracted. (b) When...

  15. 30 CFR 1206.260 - Allocation of washed coal.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false Allocation of washed coal. 1206.260 Section... RESOURCES REVENUE PRODUCT VALUATION Federal Coal § 1206.260 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was extracted. (b) When...

  16. Association between antral follicle count and reproductive measures in New Zealand lactating dairy cows maintained in a pasture-based production system.

    PubMed

    Martinez, Marcelo F; Sanderson, Neil; Quirke, Laurel D; Lawrence, Stephen B; Juengel, Jennifer L

    2016-02-01

    The antral follicle count (AFC) in cattle is consistent throughout the estrous cycle of individual cows, and cows with a lower AFC have lower fertility. We assessed the AFC at random stages of the estrous cycle, examined the correlation between AFC classifications, and determined the relationship between the most rapid and practical laboratory-based AFC classification (AFC of follicles of ≥ 2 mm in diameter) and fertility measures in New Zealand lactating dairy cows. Cows detected in estrus (n = 202) or not (n = 239) during the first 4 weeks of the breeding season were subjected to ultrasonography and classified as having a high, medium, or low AFC at the time of scanning (on-site classification). Images from ultrasound scanning were recorded onto video for accurate follicle counting in an imaging laboratory. A strong association (P < 0.05) between the AFC of follicles with a diameter of 2 mm or greater and fertility was observed. Cows with a high AFC had a shorter (P < 0.05) interval from calving to conception by artificial insemination (AI; 82.4 ± 1.6 vs. 87.3 ± 1.2 days) and greater pregnancy rates (PRs; i.e., PR to the first AI [68.1% vs. 45.3%], 6-week PR [81.9% vs. 67.3%], and overall PR [91.3% vs. 79.7%]) than cows with a low AFC. The AFC was positively associated (P < 0.0001) with age. Progesterone concentrations during diestrus were greater (P < 0.05) in high-AFC cows (7.6 ± 0.3 ng/mL) than in low-AFC cows (6.5 ± 0.3 ng/mL), whether these were pregnant (7.7 ± 0.3 ng/mL) or not (6.3 ± 0.2 ng/mL). A rapid on-site scoring system determined that cows classified as having a high AFC had a shorter (P < 0.05) interval from calving to the first AI (76.5 ± 1.7 vs. 82.3 ± 1.9 days) and were more likely to show estrus (P < 0.01; 56.8% vs. 36.4%) and have a CL at the beginning of the breeding season (P < 0.01; 93.4% vs. 79.6%) than cows with a low on-site AFC. Collectively, we have confirmed an association between AFC2 and fertility, and these results

  17. 30 CFR 206.459 - Allocation of washed coal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Allocation of washed coal. 206.459 Section 206... MANAGEMENT PRODUCT VALUATION Indian Coal § 206.459 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was extracted. (b) When the net...

  18. 30 CFR 206.260 - Allocation of washed coal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Allocation of washed coal. 206.260 Section 206... MANAGEMENT PRODUCT VALUATION Federal Coal § 206.260 Allocation of washed coal. (a) When coal is subjected to washing, the washed coal must be allocated to the leases from which it was extracted. (b) When the net...

  19. Assessment of the innate immune response in the periparturient cow.

    PubMed

    Trevisi, Erminio; Minuti, Andrea

    2018-02-01

    The transition period is the most critical phase in the life of high yielding dairy cows. Within a few weeks, cows are submitted to many challenges (physiological, nutritional, psychological, management) that require prompt and effective adaptive responses. The immune system is involved in this process, and many changes of the cow's immune system components have been observed around calving. Cows are considered to be immunosuppressed in late lactation, and available data suggest that the immune system is dysregulated around parturition. Significant attention has been focused on modification of cellular functions (e.g. the reduction of phagocytosis and diapedesis), but growing interest concerns the components of the innate immune system, which often exhibits increased responses such as susceptibility to inflammatory events and the related acute phase response (APR). Systemic inflammation plays a significant role in early lactation, affects many liver functions and has been associated with the impairment of cow performance (i.e. reduced feed intake, milk yield, fertility, welfare). The assessment of variations in immune-metabolic indices offers opportunities to predict the onset of the health troubles and to anticipate the proper therapies needed to guarantee health, good welfare and fertility in the following lactation. The frequency of diseases (metabolic and infectious) before calving is rare, but several clues suggest that various metabolic and immune variations can begin during the dry period. Interesting preliminary results encourage this perspective and possible candidates are suggested. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Whole cow's milk in infancy.

    PubMed

    Leung, Alexander Kc; Sauve, Reginald S

    2003-09-01

    Early introduction of whole cow's milk may lead to iron deficiency anemia. From a nutritional point of view, it is best to delay the introduction of whole cow's milk until the infant is one year old. While there is no evidence to suggest adverse clinical sequelae associated with the increased renal solute load in healthy infants, feeding with whole cow's milk would narrow the margin of safety in situations that may lead to dehydration. Early exposure to cow's milk proteins increases the risk of developing allergy to milk proteins. Because of the possible association between early exposure to cow's milk proteins and risk for type 1 diabetes mellitus, breast-feeding and avoidance of commercially available cow's milk and products containing intact cow's milk protein during the first year of life are strongly encouraged in families with a strong history of insulin dependent diabetes mellitus. The authors suggest that the optimal food in infancy is human breast milk. If human milk is not available, it is preferred that iron-fortified formulas rather than whole cow's milk be used during the first year of life.

  1. Hand washing compliance among retail food establishment workers in Minnesota.

    PubMed

    Allwood, Paul B; Jenkins, Timothy; Paulus, Colleen; Johnson, Lars; Hedberg, Craig W

    2004-12-01

    Inadequate hand washing by food workers is an important contributing factor to foodborne disease outbreaks in retail food establishments (RFEs). We conducted a survey of RFEs to investigate the effect of hand washing training, availability of hand washing facilities, and the ability of the person in charge (PIC) to describe hand washing according to the Minnesota Food Code (food code) on workers' ability to demonstrate food code-compliant hand washing. Only 52% of the PICs could describe the hand washing procedure outlined in the food code, and only 48% of workers could demonstrate code-compliant hand washing. The most common problems observed were failure to wash for 20 s and failure to use a fingernail brush. There was a strong positive association between the PIC being a certified food manager and being able to describe the food code hand washing procedure (odds ratio [OR], 5.5; 95% confidence interval [CI], 2.2 to 13.7), and there was an even stronger association between the PIC being able to describe hand washing and workers being able to demonstrate code-compliant hand washing (OR, 15; 95% CI, 6 to 37). Significant associations were detected among correct hand washing demonstration, physical infrastructure for hand washing, and the hand washing training methods used by the establishment. However, the principal determinant of successful hand washing demonstration was the PIC's ability to describe proper hand washing procedure. These results suggest that improving hand washing practices among food workers will require interventions that address PIC knowledge of hand washing requirement and procedure and the development and implementation of effective hand washing training methods.

  2. Influence of cow breed type, age and previous lactation status on cow height, calf growth, and patterns of body weight, condition, and blood metabolites for cows grazing bahiagrass pastures.

    PubMed

    Coleman, S W; Chase, C C; Riley, D G; Williams, M J

    2017-01-01

    This study was initiated to evaluate performance and patterns of cow traits and blood metabolites of 3 breeds of cows grazing bahiagrass (Paspalum notatum Flügge) pastures in central Florida. Purebred cows (n = 411) of either Angus (Bos taurus), Brahman (Bos indicus), or Romosinuano (Bos taurus) breeding, rotationally grazed (moved twice weekly) bahiagrass pastures year-round, and received bahiagrass hay supplemented with molasses and soyhulls or legume hay supplemented with unfortified molasses from October to June each production year. At monthly intervals, all cows were weighed, measured at the hip (HH), scored for BCS, and blood samples collected by jugular puncture from 10 cows per cow breed/block group for plasma urea N (PUN), glucose and non-esterified fatty acids (NEFA). Data were analyzed on cows that calved with a statistical model that included fixed effects of year, cowage, cow breed, month, block, supplement group (n = 2, but not presented), and whether the cow weaned a calf the previous year. Cow was a repeated observation over mo. Three-way interactions involving monthly patterns for cowage x year, year x lactation status the previous year, cowage × cow breed, year × cow breed, and cow breed × lactation status the previous year were significant (P < 0.001) for BW and BCS. The interaction for cowage × month was also significant (P < 0.05) for glucose, and cow breed × month was important (P < 0.01) for PUN, glucose, and NEFA. Important differences included: 1) greater BW and BCS for older cows compared to 3-yr old cows; 2) greater BW and BCS before calving for cows that did not lactate the previous year; 3) PUN levels were above 11 mg/dl except for February, August and September, and was generally greater in tropically adapted breeds; 4) GLU was greatest in Brahman, lowest in Angus, and intermediate in Romosinuano cows; and 5) plasma levels of NEFA escalated at calving and then declined, but Brahman cows maintained greater (P < 0.05) levels from

  3. Eye wash water flow direction study: an evaluation of the effectiveness of eye wash devices with opposite directional water flow.

    PubMed

    Fogt, Jennifer S; Jones-Jordan, Lisa A; Barr, Joseph T

    2018-01-01

    New designs of eye wash stations have been developed in which the direction of water flow from the fountain has been reversed, with two water streams originating nasally in both eyes and flowing toward the temporal side of each eye. No study has been done to determine the ideal direction of water flow coming from the eye wash in relation to the eye. Ophthalmic eye examinations were conducted before and after the use of two eye wash stations with opposite water flow directionality. Fluorescein was instilled in both eyes before using an eye wash to measure the effectiveness of the water flow. Subjects were surveyed upon their experiences using the eye washes. Ophthalmic examination found no significant difference in the efficacy of the eye washes with nasal-to-temporal water flow when compared to temporal-to-nasal water flow direction.

  4. Treatments of clinical mastitis occurring in cows on 51 large dairy herds in Wisconsin.

    PubMed

    Oliveira, L; Ruegg, P L

    2014-09-01

    Antimicrobials are frequently used for treatment of bovine mastitis and few studies have examined modern treatment strategies on large US dairy farms. The objective of this study was to describe treatment practices for clinical mastitis occurring in cows on large dairy herds in Wisconsin. Treatments performed on 747 cows experiencing cases of mild, moderate, or severe symptoms of clinical mastitis were recorded on 51 Wisconsin dairy farms. Duplicate milk samples were collected from the affected quarter for microbiological analysis at the onset of clinical mastitis and 14 to 21 d after treatment ended. Cows were treated according to individual farm protocol. Drugs and doses used for treatments were recorded for each case. Among all herds, 5 intramammary (IMM) antimicrobials (amoxicillin, hetacillin, pirlimycin, ceftiofur, and cephapirin) were used to treat cows for clinical mastitis. Of 712 cows with complete treatment data, 71.6% were treated with IMM ceftiofur either solely or combined with other antimicrobials (administered either IMM or systemically). Of cows experiencing severe symptoms of clinical mastitis, 43.8% received IMM treatment concurrent with systemic antimicrobials. Of all cows treated, 23.1% received an additional secondary treatment (either IMM, systemic, or both) because of perceived lack of response to the initial treatment. The majority of IMM treatments were administered to cows with a microbiological diagnosis of no growth (34.9%) or Escherichia coli (27.2%). Half of the cows experiencing cases caused by E. coli were treated using systemic antimicrobials in contrast to only 6.8% of cows experiencing cases caused by coagulase-negative staphylococci. In conflict with FDA regulations, which do not allow extra-label treatments using sulfonamides, a total of 22 cows from 8 farms were treated with systemic sulfadimethoxine either solely or in combination with oxytetracycline. Antimicrobial drugs were used on all herds and many cows received extra

  5. Developing a multi-Kinect-system for monitoring in dairy cows: object recognition and surface analysis using wavelets.

    PubMed

    Salau, J; Haas, J H; Thaller, G; Leisen, M; Junge, W

    2016-09-01

    Camera-based systems in dairy cattle were intensively studied over the last years. Different from this study, single camera systems with a limited range of applications were presented, mostly using 2D cameras. This study presents current steps in the development of a camera system comprising multiple 3D cameras (six Microsoft Kinect cameras) for monitoring purposes in dairy cows. An early prototype was constructed, and alpha versions of software for recording, synchronizing, sorting and segmenting images and transforming the 3D data in a joint coordinate system have already been implemented. This study introduced the application of two-dimensional wavelet transforms as method for object recognition and surface analyses. The method was explained in detail, and four differently shaped wavelets were tested with respect to their reconstruction error concerning Kinect recorded depth maps from different camera positions. The images' high frequency parts reconstructed from wavelet decompositions using the haar and the biorthogonal 1.5 wavelet were statistically analyzed with regard to the effects of image fore- or background and of cows' or persons' surface. Furthermore, binary classifiers based on the local high frequencies have been implemented to decide whether a pixel belongs to the image foreground and if it was located on a cow or a person. Classifiers distinguishing between image regions showed high (⩾0.8) values of Area Under reciever operation characteristic Curve (AUC). The classifications due to species showed maximal AUC values of 0.69.

  6. Effect of different flooring systems on weight and pressure distribution on claws of dairy cows.

    PubMed

    Telezhenko, E; Bergsten, C; Magnusson, M; Ventorp, M; Nilsson, C

    2008-05-01

    Weight and pressure distribution on the claw were studied in Swedish Holsteins housed in different flooring systems. A total of 127 cows housed in different sections of the experimental barn were used. Each section had different flooring in the walking and standing areas. There were rubber mats or abrasive mastic asphalt flooring on the alleys or a low-abrasive slatted concrete floor. Some sections had feed-stalls equipped with rubber mats; other sections did not. The vertical ground reaction force, contact area, and average contact pressure were determined on the left hind foot using the I-Scan system and analyzed with the F-scan system. These determinations were made in each of the following 3 zones of the claw: bulb, wall, and sole. Most of the weight on claws exposed to concrete floors was carried by the bulb (37.4 +/- 3.5 and 18.3 +/- 2.9% of weight exerted on the foot in the lateral and medial claw, respectively) and the wall zone (20.0 +/- 2.6 and 13.4 +/- 2.4% on lateral and medial claw, respectively). The weight and pressure distribution in cows kept on sections with rubber covered alleys but passing daily over the asphalt floor on their way to the milking parlor did not differ in any zones, except for lateral bulbs, compared with those exposed to slatted concrete alone. Still, the weight bearing of the sole zone in cows kept on rubber mats without access to asphalt was less than that of cows kept on concrete slatted floors (5.1 +/- 0.7 vs. 12.7 +/- 1.1% and 1.1 +/- 0.5 vs. 8.7 +/- 0.7% in lateral and medial claws, respectively). In cows kept on asphalt flooring without feed-stalls, most weight was exerted to the sole zone (36.2 +/- 2.9 and 22.2 +/- 1.8% in lateral and medial claws, respectively). Feed-stalls in combination with asphalt flooring yielded a decreased total contact area (30.1 +/- 1.2 cm(2)) compared with asphalt floors without feed-stalls (35.7 +/- 1.2 cm(2)). The largest total contact area was obtained on the asphalt floor without feed

  7. Cow's milk - infants

    MedlinePlus

    ... this page: //medlineplus.gov/ency/article/002448.htm Cow's milk - infants To use the sharing features on ... year old, you should not feed your baby cow's milk, according to the American Academy of Pediatrics ( ...

  8. Does body size of dairy cows, at constant ratio of maintenance to production requirements, affect productivity in a pasture-based production system?

    PubMed

    Hofstetter, P; Steiger Burgos, M; Petermann, R; Münger, A; Blum, J W; Thomet, P; Menzi, H; Kohler, S; Kunz, P

    2011-12-01

    This study compared productivity of dairy cows with different body weight (BW), but a constant ratio of maintenance to production requirements in their first lactation, in a pasture-based production system with spring calving. Two herds, Herd L (13 and 14 large cows in 2003 and 2004 respectively; average BW after calving, 721 kg) and Herd S (16 small cows in both years; 606 kg) [Correction added after online publication 14 January 2011: 16 small cows in both years; 621 kg was changed to 16 small cows in both years; 606 kg], all in their second or following lactations, were each allocated 6 ha of pasture and rotationally grazed on 10 parallel paddocks with equal herbage offer and nutritional values. Winter hay, harvested from the same pastures, was offered ad libitum in the indoor periods in a tied stall barn. Each herd received, per lactation and year, approximately 2000 kg dry matter (DM) of concentrates and of fodder beets, equally distributed to every individual. Indoors, the L-cows ingested more DM than the S-cows (18.7 vs. 16.3 kg DM/cow per day; p < 0.01), but DM intake per 100 kg of metabolic BW was similar (13.0 vs. 13.1 kg DM/cow per day). Estimates based on the n-alkane technique gave similar results on pasture (17.9 vs. 15.5 kg DM/cow per day; p < 0.001). Roughage intakes per 100 kg of metabolic BW, at 13.5 kg DM/cow per day, were similar. Mean annual yield of energy-corrected milk (ECM)/ha was slightly higher for the S-herd than the L-herd (13,026 vs. 12,284 kg) but was associated with a higher stocking rate (on average +20%) for the S-herd. Feed conversion efficiency (1.2 vs. 1.3 kg ECM/kg DM intake) and overall milk production efficiency (45.3 vs. 47.3 kg ECM/kg metabolic BW) were similar in L- and S-cows. Thus, both dairy cow types were equally efficient in utilising pasture-based forage. © 2010 Blackwell Verlag GmbH.

  9. Cross contamination of Escherichia coli O157:H7 between lettuce and wash water during home-scale washing.

    PubMed

    Jensen, Dane A; Friedrich, Loretta M; Harris, Linda J; Danyluk, Michelle D; Schaffner, Donald W

    2015-04-01

    Lettuce and leafy greens have been implicated in multiple foodborne disease outbreaks. This study quantifies cross contamination between lettuce pieces in a small-scale home environment. A five-strain cocktail of relevant Escherichia coli O157:H7 strains was used. Bacterial transfer between single inoculated lettuce leaf pieces to 10 non-inoculated lettuce leaf pieces that were washed in a stainless steel bowl of water for 30 s, 1 min, 2 min, and 5 min was quantified. Regardless of washing time, the wash water became contaminated with 90-99% of bacteria originally present on the inoculated lettuce leaf piece. The E. coli O157:H7 concentration on initially inoculated leaf pieces was reduced ∼ 2 log CFU. Each initially uncontaminated lettuce leaf piece had ∼ 1% of the E. coli O157:H7 from the inoculated lettuce piece transferred to it after washing, with more transfer occurring during the shortest (30 s) and longest (5 min) wash times. In all cases the log percent transfer rates were essentially normally distributed. In all scenarios, most of the E. coli O157:H7 (90-99%) transferred from the inoculated lettuce pieces to the wash water. Washing with plain tap water reduces levels of E. coli O157:H7 on the inoculated lettuce leaf pieces, but also spreads contamination to previously uncontaminated leaf pieces. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Treatment techniques for the recycling of bottle washing water in the soft drinks industry.

    PubMed

    Ramirez Camperos, E; Mijaylova Nacheva, P; Diaz Tapia, E

    2004-01-01

    The soft drink production is an important sector in the manufacturing industry of Mexico. Water is the main source in the production of soft drinks. Wastewater from bottle washing is almost 50% of the total wastewater generated by this industry. In order to reduce the consumption of water, the water of the last bottle rinse can be reused in to the bottle pre-rinse and pre-washing cycles. This work presents the characterization of the final bottle washing rinse discharge and the treatability study for the most appropriate treatment system for recycling. Average characteristics of the final bottle wash rinse were as follows: Turbidity 40.46 NTU, COD 47.7 mg/L, TSS 56 mg/L, TS 693.6 mg/L, electrical conductivity 1,194 microS/cm. The results of the treatability tests showed that the final rinse water can be used in the pre-rinse and pre-washing after removing the totality of the suspended solids, 80% of the COD and 75% of the dissolved solids. This can be done using the following treatment systems: filtration-adsorption-reverse osmosis, or filtration-adsorption-ion exchange. The installation of these treatment techniques in the soft drink industry would decrease bottle washing water consumption by 50%.

  11. Wash flow disturbance and summer wash flow in the Mojave Desert: Influence on dispersion, production, and physiological functioning of dominant shrubs

    NASA Astrophysics Data System (ADS)

    Newlander, April

    In many Mojave Desert ecosystems, water infiltrates to root-zones in greatest proportion via washes. As such, washes have a pronounced effect on plant dispersion and size across these landscapes. Desert roads alter the natural spatial patterns of washes on alluvial fans (locally called bajadas) and potentially affect plant production and distribution. As a winter-rainfall dominated ecosystem, climate changes in the Mojave Desert that increase summer precipitation may also play an important role in altering vegetation processes influenced by washes. Road effects on the spatial distribution of desert plants on a Mojave Desert bajada were examined using remotely sensed LiDAR data and ground based measurements of plant size. Plant physiological responses to summer wash flow were also quantified by measuring gas exchange and water status of two dominant perennial species, Larrea tridentata and Ambrosia dumosa. Larrea and Ambrosia plants were nearly 7x and 4x larger where wash flow has been enhanced by road culverts, relative to undisturbed areas and areas where flow has been cut-off by the presence of a road/railroad. Clustering of large plants occurred along wash margins, with clustering most pronounced in areas of enhanced wash flow. No clustering was found where wash flow has been eliminated. For ecophysiological traits, both species showed pronounced responses to the pulse of water; however, these responses varied as a function of distance from wash. Larrea plants within 3 m and Ambrosia plants within ca. 2 m from the wash responded to the pulse of water. Leaf phenology dictated the timing of carbon gain as Larrea experienced a rapid but short-lived increase in stomatal conductance compared to a significant response for over a month following the pulse for Ambrosia. These results indicate that disturbance of desert washes has a pronounced impact on vegetation structure, and changing climatic conditions that impact plant function could potentially lead to even

  12. Eggshell bacterial levels of non-washed and washed eggs from caged and cage-free hens

    USDA-ARS?s Scientific Manuscript database

    The bacteria levels of non-washed and washed eggs obtained from caged and cage-free laying hens housed on either all shavings or all wire slat floors were determined. On eight sample days (from 22 to 52 weeks at 4 week intervals), 20 eggs were collected from each pen (n=120/sample day). Ten eggs p...

  13. Seasonal variation in time budgets and milk yield for Jersey, Friesland and crossbred cows raised in a pasture-based system.

    PubMed

    Dodzi, Madodana S; Muchenje, Voster

    2012-10-01

    The time budgets and daily milk yield of Jersey and Friesland cows and their crosses were compared in a pasture-based system by recording the time spent grazing, drinking, lying, standing and walking in four seasons of the year (cool-dry, hot-dry, hot-wet and post-rainy). Observations were made from 0800 to 1400 hours on seven cows per breed. Seven observers monitored the cows at 10-min intervals for 6 h using stop watches. Time spent standing was higher (P < 0.05) for Friesland compared to Jersey cows and the crossbred cows during the hot-wet season. Time spent walking differed among the three genotypes with the Jersey spending more time (P < 0.05) in both hot-wet and cool-dry seasons. No differences were noted on time spent lying down (P > 0.05) across the genotypes in the hot-wet season. In the cool-dry season, differences in time spent grazing (P < 0.05) were noted with the Jersey cows spending more time. The Friesland and the crossbred spent more time lying down (P < 0.05) than the Jersey cows in the cool-dry season. No time differences were noted for time spent standing (P > 0.05) in the same season. The Jersey cows spent the longest time walking (P < 0.05) during the cool-dry period. There were seasonal differences in time spent in all activities (P < 0.05). Time spent on grazing was longest in post-rainy season and lowest in hot-wet season. Differences were observed in the time spent lying down (P < 0.05). The longest period was observed in the hot-dry season and lowest in the hot-wet season. Daily milk yield varied (P < 0.05) with breed with the Friesland and Jersey producing higher yields than the crosses. The highest amount was produced in hot-dry and the least in hot-wet season. Milk yield and lying down were positively correlated (P < 0.05) in Jersey and Friesland cows. Standing was negatively correlated with milk yield (P < 0.05) in both Friesland and Jersey cows. No significant relationship was observed for the crossbred cows. It was concluded that

  14. 33 CFR 157.166 - Hydrocarbon emissions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Hydrocarbon emissions. 157.166... Crude Oil Washing (COW) System on Tank Vessels Cow Operations § 157.166 Hydrocarbon emissions. If the... ballasted in that port the hydrocarbon vapors in each tank are contained by a means under § 157.132. Note...

  15. Lameness scoring system for dairy cows using force plates and artificial intelligence.

    PubMed

    Ghotoorlar, S Mokaram; Ghamsari, S Mehdi; Nowrouzian, I; Ghotoorlar, S Mokaram; Ghidary, S Shiry

    2012-02-04

    Lameness scoring is a routine procedure in dairy industry to screen the herds for new cases of lameness. Subjective lameness scoring, which is the most popular lameness detection and screening method in dairy herds, has several limitations. They include low intra-observer and inter-observer agreement and the discrete nature of the scores which limits its usage in monitoring the lameness. The aim of this study is to develop an automated lameness scoring system comparable with conventional subjective lameness scoring by means of artificial neural networks. The system is composed of four balanced force plates installed in a hoof-trimming box. A group of 105 dairy cows was used for the study. Twenty-three features extracted from ground reaction force (GRF) data were used in a computer training process which was performed on 60 per cent of the data. The remaining 40 per cent of the data were used to test the trained system. Repeatability of the lameness scoring system was determined by GRF samples from 25 cows, captured at two different times from the same animals. The mean sd was 0.31 and the mean coefficient of variation was 14.55 per cent, which represents a high repeatability in comparison with subjective vision-based scoring methods. Although the highest sensitivity and specificity values were seen in locomotion score groups 1 and 4, the automatic lameness system was both sensitive and specific in all groups. The sensitivity and specificity were higher than 72 per cent in locomotion score groups 1 to 4, and it was 100 per cent specific and 50 per cent sensitive for group 5.

  16. Sugarcane bagasse as exclusive roughage for dairy cows in smallholder livestock system.

    PubMed

    de Almeida, Gleidiana Amélia Pontes; de Andrade Ferreira, Marcelo; de Lima Silva, Janaina; Chagas, Juana Catarina Cariri; Véras, Antônia Sherlânea Chaves; de Barros, Leonardo José Assis; de Almeida, Gledson Luiz Pontes

    2018-03-01

    The study aimed to evaluate sugarcane bagasse as roughage in lactating cow on feed intake, digestibility, ingestive behavior, milk production and composition, and microbial protein synthesis. Ten Girolando cows at initial body weight of 450±25.6 kg and at 143.7±30.7 days in milk were assigned in two 5×5 Latin square designs. Five 21-day experimental periods were adopted (1° to 14-day: diets adaptation period; 15° to 21-day: data collection and sampling period). The diets consisted of four different levels of sugarcane bagasse (45%, 50%, 55%, and 60%) and a control diet, commonly adopted in the region, based on spineless cactus (25% sugarcane bagasse), formulated to meet 12 kg/d milk yield. The dry matter (DM), organic matter (OM), and total digestible nutrients intakes and DM and OM digestibilities observed for 45% and 50% bagasse inclusion were similar to control diet, while that 55% and 60% bagasse inclusion were lower. Cows fed control diet, and bagasse diets of 45%, and 50% levels had the nutritional requirements attended, that guaranteed 12 kg/d of milk yield. The crude protein intake and digestibility of cows fed 45%, 50%, and 55% of bagasse inclusion were similar to control diet. The neutral detergent fiber (NDF) intake and digestibility differ for all bagasse diets related to control diet, while the non-fiber carbohydrates intake and digestibility for cows fed 45% of bagasse were similar for control diet. The intakes and digestibilities of nutrients decreased linearly in function of bagasse inclusion; NDF and indigestible NDF intakes did not vary. The ruminating time, feeding and rumination efficiency, microbial protein synthesis and milk yield decreased linearly with sugarcane bagasse inclusion. Sugarcane bagasse decreases milk production; however, its inclusion level in between 45% to 50% associated to concentrate could replace diets based on spineless cactus for crossbred dairy cow's producing 12 kg/d of milk.

  17. Sugarcane bagasse as exclusive roughage for dairy cows in smallholder livestock system

    PubMed Central

    2018-01-01

    Objective The study aimed to evaluate sugarcane bagasse as roughage in lactating cow on feed intake, digestibility, ingestive behavior, milk production and composition, and microbial protein synthesis. Methods Ten Girolando cows at initial body weight of 450±25.6 kg and at 143.7±30.7 days in milk were assigned in two 5×5 Latin square designs. Five 21-day experimental periods were adopted (1° to 14-day: diets adaptation period; 15° to 21-day: data collection and sampling period). The diets consisted of four different levels of sugarcane bagasse (45%, 50%, 55%, and 60%) and a control diet, commonly adopted in the region, based on spineless cactus (25% sugarcane bagasse), formulated to meet 12 kg/d milk yield. Results The dry matter (DM), organic matter (OM), and total digestible nutrients intakes and DM and OM digestibilities observed for 45% and 50% bagasse inclusion were similar to control diet, while that 55% and 60% bagasse inclusion were lower. Cows fed control diet, and bagasse diets of 45%, and 50% levels had the nutritional requirements attended, that guaranteed 12 kg/d of milk yield. The crude protein intake and digestibility of cows fed 45%, 50%, and 55% of bagasse inclusion were similar to control diet. The neutral detergent fiber (NDF) intake and digestibility differ for all bagasse diets related to control diet, while the non-fiber carbohydrates intake and digestibility for cows fed 45% of bagasse were similar for control diet. The intakes and digestibilities of nutrients decreased linearly in function of bagasse inclusion; NDF and indigestible NDF intakes did not vary. The ruminating time, feeding and rumination efficiency, microbial protein synthesis and milk yield decreased linearly with sugarcane bagasse inclusion. Conclusion Sugarcane bagasse decreases milk production; however, its inclusion level in between 45% to 50% associated to concentrate could replace diets based on spineless cactus for crossbred dairy cow's producing 12 kg/d of milk

  18. Ability of dairy cows to be inseminated according to breed and genetic merit for production traits under contrasting pasture-based feeding systems.

    PubMed

    Bedere, N; Disenhaus, C; Ducrocq, V; Leurent-Colette, S; Delaby, L

    2017-05-01

    Strong genetic selection on production traits is considered to be responsible for the declined ability of dairy cows to ensure reproduction. The present study aimed to quantify the effect of genetic characteristics (breeds and genetic merit for production traits) and feeding systems (FS) on the ability of dairy cows to be inseminated. An experiment was conducted during 9 years on Normande and Holstein cows assigned to contrasted pasture-based FS. Diets were based on maize silage in winter and grazing plus concentrate in spring in the High FS; and on grass silage in winter and grazing with no concentrate during spring in the low FS. Within breed, cows were classified into two genetic groups with similar estimated breeding values (EBV) for milk solids: cows with high EBV for milk yield were included in a Milk-Group and those with high EBV for fat and protein contents were included in a Content-Group. Holstein produced more milk throughout lactation than Normande cows (+2294 kg in the High FS and +1280 kg in the Low FS, P<0.001) and lost more body condition to nadir (-1.00 point in the High FS and -0.80 kg in the Low FS, P<0.001). They also showed a poorer ability to be inseminated because of both a delayed commencement of luteal activity (CLA) and delayed first service (more days from start of the breeding season to first service, DAI1). Cows in the Milk-Group produced more milk than cows in the Content-Group, but milk solids production was similar. Cows in the Content-Group had earlier CLA than cows in the Milk-Group (P<0.01). Genetic group neither affected ovulation detection rate nor DAI1. Within breed and FS, cows with high genetic merit for milk yield had later CLA and DAI1. Cows in the High FS produced more milk and lost less condition to nadir than cows in the Low FS. FS did not affect dairy cows' ability to be inseminated. However, cows with higher milk protein content, and presumably better energy balance, had earlier CLA (P<0.01) and DAI1 (P<0.10). In

  19. The effect of floor surface on dairy cow immune function and locomotion score

    USDA-ARS?s Scientific Manuscript database

    The study evaluated the effect of 2 dairy cow housing systems on cow locomotion, leukocyte activity and expression of genes associated with lameness, during the dry and peri-parturient period. Cows were assigned to free-stall housing with either rubber (RUB; n=13) or concrete (CON; n=14) at the feed...

  20. Domestic wash water reclamation for reuse as commode water supply using filtration: Reverse-osmosis separation technique

    NASA Technical Reports Server (NTRS)

    Hall, J. B., Jr.; Batten, C. E.; Wilkins, J. R.

    1974-01-01

    A combined filtration-reverse-osmosis water recovery system has been evaluated to determine its capability to reclaim domestic wash water for reuse as a commode water supply. The system produced water that met all chemical and physical requirements established by the U.S. Public Health Service for drinking water with the exception of carbon chloroform extractables, methylene blue active substances, and phenols. It is thought that this water is of sufficient quality to be reused as commode supply water. The feasibility of using a combined filtration and reverse-osmosis technique for reclaiming domestic wash water has been established. The use of such a technique for wash-water recovery will require a maintenance filter to remove solid materials including those less than 1 micron in size from the wash water. The reverse-osmosis module, if sufficiently protected from plugging, is an attractive low-energy technique for removing contaminants from domestic wash water.

  1. Evaporative cooling for Holstein dairy cows under grazing conditions

    NASA Astrophysics Data System (ADS)

    Valtorta, Silvia E.; Gallardo, Miriam R.

    . Twenty-four grazing Holstein cows in mid and late lactation were randomly assigned to two treatment groups: control and cooled. The trial was performed at the Experimental Dairy Unit, Rafaela Agricultural Experimental Station (INTA), Argentina. The objective was to evaluate the effects of sprinkler and fan cooling before milkings on milk production and composition. The effects of the cooling system on rectal temperature and respiration rate were also evaluated. Cooled cows showed higher milk production (1.04 l cow-1 day-1). The concentration and yield of milk fat and protein increased in response to cooling treatment. The cooling system also reduced rectal temperature and respiration rate. No effects were observed on body condition. It was concluded that evaporative cooling, which is efficient for housed animals, is also appropriate to improve yields and animal well-being under grazing systems. These results are impressive since the cooling system was utilized only before milkings, in a system where environmental control is very difficult to achieve. This trial was performed during a mild summer. The results would probably be magnified during hotter weather.

  2. Comparison of stored red blood cell washing techniques for priming extracorporeal circuits.

    PubMed

    Sasaki, Jun; Tirotta, Christopher; Lim, Hyunsoo; Kubes, Kathleen; Salvaggio, Jane; Hannan, Robert; Burke, Redmond; Ojito, Jorge

    2018-03-01

    The aim of this study was to compare three different blood washing techniques and describe the differences for the composition of the washed red blood cells (RBC). Stored RBCs less than 5 days old were washed using three different techniques. 1) Washing with normal saline with the COBE Model 2991 blood processor in the blood bank (BB-S). 2) Washing with normal saline with the Continuous AutoTransfusion System (C.A.T.S) in the operating room (OR-S). 3) Washing with Plasma-Lyte with the C.A.T.S in the operating room (OR-PL). Then, we compared the values for hemoglobin (Hb), hematocrit (Hct), blood volume, RBC volume, lactate, glucose, sodium and potassium of the three different groups. Forty-five units of RBCs were washed and analyzed (15 for each technique). The OR-S RBCs, when compared to the BB-S RBCs, had lower hemoglobin (g/dL) (22.8 vs 24.1, p=0.006), lower hematocrit (%) (67 vs 71, p=0.006), higher RBC volume (ml) (161 vs 130, p<0.001), higher glucose (mg/dL) (185 vs 46, p<0.001) and lower sodium (mmol/L) (153 vs 158, p<0.001). When compared to the OR-S RBCs, the OR-PL RBCs showed higher potassium (mmol/L) (5.3 vs 2, p<0.001) and lower sodium (mmol/L) (129 vs 153, p<0.001). RBCs washed with an autotransfusion device had a higher RBC volume and more physiological levels of glucose and sodium when compared with the blood processor in the blood bank. It can be an alternative option to use RBCs washed with an autotransfusion device for priming the extracorporeal circuits utilized in patients undergoing cardiac surgery.

  3. Evaluation of F1 cows sired by Brahman, Boran, and Tuli bulls for reproductive, maternal, and cow longevity traits.

    PubMed

    Muntean, C T; Herring, A D; Riley, D G; Gill, C A; Sawyer, J E; Sanders, J O

    2018-05-12

    This study evaluated reproductive, maternal performance, and longevity traits of 143 F1 cows sired by Brahman (Br), Boran (Bo), or Tuli (T) bulls from Angus or Hereford cows from 1994 to 2011. Cow traits were measured at 7 yr of age in 1999 and 2000 for 1992- and 1993-born cows, respectively. From 2004 to 2010, excluding 2008, incisor condition (solid, broken, smooth) and condition scores were assigned to cows remaining in production; scores were evaluated with two models. Broken and solid mouths were each assigned a score of 1, and smooth assigned "0", Br- (0.76) and Bo-sired cows (0.71) had higher scores (P < 0.05) than T-sired cows (0.54). When solid mouths were scored 1 and smooth and broken scored 0, Br-sired cows (0.34) were higher than T (0.01) (P < 0.05), and Bo (0.23) sired cows were not different from either (P > 0.05). Age level of the cow within birth year was important for all modeled calf traits (P < 0.05). Birth weights were not different among cow inheritance (P > 0.05). Cow type influenced (P < 0.05) 205-d adjusted weaning weight of calves; Br-sired dams (228.1 ± 2.37 kg) produced the greatest weaning weight, followed by Bo- (213.7 ± 3.10 kg), and T- (201.6 ± 2.69 kg) sired dams (P < 0.05). Adjusted means for calving rate for Bo-sired (0.92 ± 0.02) cows were higher (P < 0.05) than Br- (0.86 ± 0.02) and T- sired (0.86 ± 0.02) cows. Adjusted mean weaning rate was greater (P < 0.05) for Bo-sired cows (0.86 ± 0.02) than for cows sired by Br (0.77 ± 0.02) bulls, but weaning rate for T-sired cows (0.80 ± 0.02) were similar (P > 0.05). Cow weight was greater (P < 0.05) for Br-sired cows (590.5 ± 8.35 kg), than for Bo-sired (505.8 ± 10.46 kg) or T-sired cows (508.5 ± 9.37 kg). Body condition score at weaning for 7-yr-old cows was similar (P = 0.08) for Br-sired and Bo-sired cows and lower for T-sired cows (P = 0.0005, condition scores 6.0, 6.3, and 5.8, respectively). Bo-sired cows were older when they were removed from the herd, on average (12

  4. Wash or wipe? A comparative study of skin physiological changes between water washing and wiping after skin cleaning.

    PubMed

    Ogai, K; Matsumoto, M; Aoki, M; Ota, R; Hashimoto, K; Wada, R; Kobayashi, M; Sugama, J

    2017-11-01

    Presently, skin-cleaning agents that claim to be removed by water or wiping alone are commercially available and have been used for the purpose of bed baths. However, there is a lack of knowledge on how water washing and wiping differently affect skin physiological functions or ceramide content. The aim of this study was to compare the effects of water washing and wiping on skin physiological functions and ceramide content. Three kinds of the cleaning agents with different removal techniques (ie, water washing and wiping) were used in this study. Skin physiological functions (ie, transepidermal water loss, skin hydration, and skin pH) and skin ceramide content were measured before and after seven consecutive days of the application of each cleaning agent. No significant differences in skin physiological functions or ceramide content were observed between water washing and wiping. Cleaning agents that claim to be removed by water washing or wiping do not affect skin physiological functions or ceramide content by either removal method. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  5. Using Olfaction and Unpleasant Reminders to Reduce the Intention-behavior Gap in Hand Washing

    PubMed Central

    Pellegrino, Robert; Crandall, Philip G.; Seo, Han-Seok

    2016-01-01

    Lack of hand washing is a leading cause of food borne illnesses. To successfully increase hand hygiene compliance, interventions must have continual engagement with employees. This study used a real-time prospective memory (PM) scenario to measure the effectiveness of a control and sensory reminders of disgust to influence hand washing behavior and performance. First, a model of hand washing performance was built by having six participants’ hands contaminated with GermGlo (a florescent micro-particle) and then washed their hands using predetermined protocols while monitored by an electronic hand hygiene verification (HHV) system. Next, eighty Hispanic/Latino participants, in a between-group experimental design, performed a PM experiment while one of four reminders were present (hand washing poster, disgusting image, disgusting sound, and disgusting odor) as the HHV recorded their hand washing performance. Visual cues, typical of hand washing campaigns, were not as effective at increasing hand hygiene compliance as disgust-induced sensory cues. Furthermore, olfactory disgust showed a significantly higher probability that individuals would engage in hand washing behaviors than all other conditions. This study provides new insight into the effectiveness of different senses and emotion to reduce the intention-behavior gap associated with modifying behaviors, and broadens current PM research to a real-time application. PMID:26732033

  6. Symptoms, Diagnosis, and Treatment of Cow's Milk Allergy.

    PubMed

    Vandenplas, Yvan; Marchand, Johan; Meyns, Lien

    2015-01-01

    The diagnosis and management of cow's milk allergy (CMA) is a topic of debate because there are no specific symptoms and reliable diagnostic tests. Literature was searched using databases to find original papers and reviews on this topic. A "challenge test" is still recommended as the most specific and sensitive diagnostic test, although a positive challenge test does not proof the involvement of the immune system. Only in the rare case of anaphylaxis, the challenge tests is not recommended. The Cow's Milk Symptom Score (CoMiSS) is an awareness tool for health care professionals to better recognize symptoms caused by the ingestion of cow's milk, but still needs validation. Today, the recommended diagnostic approach consists of an elimination diet with an extensive cow's milk based hydrolysate, followed by a challenge test. Amino acid based formula is reserved for the severe cases. Rice hydrolysates are gaining popularity, because they have been shown to be effective, have a better acceptability, and to be cheaper than the extensive cow's milk based hydrolysates. Soy infant formula may offer an additional alternative option as reviews concluded that about 10-15% of infants allergic to cow's milk will also react to soy. Gastro-intestinal microbiota manipulation may be of additional benefit in the treatment of CMA. The development of rice hydrolysates challenge the use of Today, extensive cow's milk based extensive hydrolysates remain the first option for the majority of patients and amino acid formula for the most severe cases. Rice hydrolysates and soy infant formula are considered as second choice options.

  7. Development assessment of wash water reclamation

    NASA Technical Reports Server (NTRS)

    Putnam, D. F.

    1976-01-01

    An analytical study assessment of state-of-the-art wash water reclamation technology is presented. It covers all non-phase-change unit operations, unit processes and subsystems currently under development by NASA. Each approach to wash water reclamation is described in detail. Performance data are given together with the projected weights and sizes of key components and subsystems. It is concluded that a simple multifiltration subsystem composed of surface-type cartridge filters, carbon adsorption and ion exchange resins is the most attractive approach for spacecraft wash water reclamation in earth orbital missions of up to 10 years in duration.

  8. Differences during the first lactation between cows cloned by somatic cell nuclear transfer and noncloned cows.

    PubMed

    Montazer-Torbati, F; Boutinaud, M; Brun, N; Richard, C; Neveu, A; Jaffrézic, F; Laloë, D; LeBourhis, D; Nguyen, M; Chadi, S; Jammes, H; Renard, J-P; Chat, S; Boukadiri, A; Devinoy, E

    2016-06-01

    Lactation performance is dependent on both the genetic characteristics and the environmental conditions surrounding lactating cows. However, individual variations can still be observed within a given breed under similar environmental conditions. The role of the environment between birth and lactation could be better appreciated in cloned cows, which are presumed to be genetically identical, but differences in lactation performance between cloned and noncloned cows first need to be clearly evaluated. Conflicting results have been described in the literature, so our aim was to clarify this situation. Nine cloned Prim' Holstein cows were produced by the transfer of nuclei from a single fibroblast cell line after cell fusion with enucleated oocytes. The cloned cows and 9 noncloned counterparts were raised under similar conditions. Milk production and composition were recorded monthly from calving until 200d in milk. At 67d in milk, biopsies were sampled from the rear quarter of the udder, their mammary epithelial cell content was evaluated, and mammary cell renewal, RNA, and DNA were then analyzed in relevant samples. The results showed that milk production did not differ significantly between cloned and noncloned cows, but milk protein and fat contents were less variable in cloned cows. Furthermore, milk fat yield and contents were lower in cloned cows during early lactation. At around 67 DIM, milk fat and protein yields, as well as milk fat, protein, and lactose contents, were also lower in cloned cows. These lower yields could be linked to the higher apoptotic rate observed in cloned cows. Apoptosis is triggered by insulin-like factor growth binding protein 5 (IGFBP5) and plasminogen activator inhibitor (PAI), which both interact with CSN1S2. During our experiments, CSN1S2 transcript levels were lower in the mammary gland of cloned cows. The mammary cell apoptotic rate observed in cloned cows may have been related to the higher levels of DNA (cytosine-5

  9. Washing of waste prior to landfilling.

    PubMed

    Cossu, Raffaello; Lai, Tiziana

    2012-05-01

    The main impact produced by landfills is represented by the release of leachate emissions. Waste washing treatment has been investigated to evaluate its efficiency in reducing the waste leaching fraction prior to landfilling. The results of laboratory-scale washing tests applied to several significant residues from integrated management of solid waste are presented in this study, specifically: non-recyclable plastics from source separation, mechanical-biological treated municipal solid waste and a special waste, automotive shredded residues. Results obtained demonstrate that washing treatment contributes towards combating the environmental impacts of raw wastes. Accordingly, a leachate production model was applied, leading to the consideration that the concentrations of chemical oxygen demand (COD) and total Kjeldahl nitrogen (TKN), parameters of fundamental importance in the characterization of landfill leachate, from a landfill containing washed wastes, are comparable to those that would only be reached between 90 and 220years later in the presence of raw wastes. The findings obtained demonstrated that washing of waste may represent an effective means of reducing the leachable fraction resulting in a consequent decrease in landfill emissions. Further studies on pilot scale are needed to assess the potential for full-scale application of this treatment. Copyright © 2012 Elsevier Ltd. All rights reserved.

  10. Algaemia in a dairy cow by Prototheca blaschkeae.

    PubMed

    Thompson, Gertrude; Silva, Eliane; Marques, Sara; Müller, Alexandra; Carvalheira, Júlio

    2009-01-01

    We describe the first known case of an algaemia by Prototheca blaschkeae in a dairy cow, which occurred after a chronic episode of mastitis caused by this pathogen. The organism was isolated from milk, joint fluid and blood samples, and microbiologic and molecular methods were performed to obtain a definitive identification of the algae. The affected cow was culled only after confirmation of a systemic infection by Prototheca.

  11. Cow-specific treatment of clinical mastitis: an economic approach.

    PubMed

    Steeneveld, W; van Werven, T; Barkema, H W; Hogeveen, H

    2011-01-01

    Under Dutch circumstances, most clinical mastitis (CM) cases of cows on dairy farms are treated with a standard intramammary antimicrobial treatment. Several antimicrobial treatments are available for CM, differing in antimicrobial compound, route of application, duration, and cost. Because cow factors (e.g., parity, stage of lactation, and somatic cell count history) and the causal pathogen influence the probability of cure, cow-specific treatment of CM is often recommended. The objective of this study was to determine if cow-specific treatment of CM is economically beneficial. Using a stochastic Monte Carlo simulation model, 20,000 CM cases were simulated. These CM cases were caused by Streptococcus uberis and Streptococcus dysgalactiae (40%), Staphylococcus aureus (30%), or Escherichia coli (30%). For each simulated CM case, the consequences of using different antimicrobial treatment regimens (standard 3-d intramammary, extended 5-d intramammary, combination 3-d intramammary+systemic, combination 3-d intramammary+systemic+1-d nonsteroidal antiinflammatory drugs, and combination extended 5-d intramammary+systemic) were simulated simultaneously. Finally, total costs of the 5 antimicrobial treatment regimens were compared. Some inputs for the model were based on literature information and assumptions made by the authors were used if no information was available. Bacteriological cure for each individual cow depended on the antimicrobial treatment regimen, the causal pathogen, and the cow factors parity, stage of lactation, somatic cell count history, CM history, and whether the cow was systemically ill. Total costs for each case depended on treatment costs for the initial CM case (including costs for antibiotics, milk withdrawal, and labor), treatment costs for follow-up CM cases, costs for milk production losses, and costs for culling. Average total costs for CM using the 5 treatments were (US) $224, $247, $253, $260, and $275, respectively. Average probabilities

  12. Perspectives on pasture versus indoor feeding of dairy cows.

    PubMed

    Knaus, Wilhelm

    2016-01-15

    The dairy industry in many regions of the world has moved towards a high-input/high-output system maximising annual milk production per cow, primarily through increasing concentrate-based total mixed rations fed indoors year round, as opposed to allowing cows to feed on pasture. Pasture-based dairy systems in regions like New Zealand and Ireland are oriented towards maximum milk yield per unit of pasture, which has led to Holstein strains that are 50 to 100 kg lighter, exhibit a higher body condition score, and produce roughly half the annual amount of milk as compared to their Holstein counterparts kept in confinement in North America and Europe. Freedom from hunger might not be guaranteed when high-yielding dairy cows are kept on pasture without any supplemental feed, but at the same time no access to pasture can be considered an animal welfare concern, because pasturing is generally beneficial to the animals' health. On pasture, lighter-weight dairy cows with a medium milk production potential have proven to be superior with regard to feed efficiency and fertility. The year-round indoor feeding of high-yielding dairy cows with total mixed rations containing substantial amounts of human-edible crops from arable land puts global food security at risk and fails to utilise the evolutionary advantages of ruminants. © 2015 Society of Chemical Industry.

  13. Bacteriological Aspects of Hand Washing: A Key for Health Promotion and Infections Control

    PubMed Central

    Ataee, Ramezan Ali; Ataee, Mohammad Hosein; Mehrabi Tavana, Ali; Salesi, Mahmud

    2017-01-01

    The aim of this review is to show the historical aspects of hands washing for healthy life and explains how can reduce the transmission of community-acquired infectious agents by healthcare workers and patients. This review article is prepared based on available database. The key words used were hands washing, risk assessment, hands hygiene, bacterial flora, contamination, infection, nosocomial, tap water, sanitizer, bacterial resistance, hands bacterial flora, washing methods, antiseptics, healthcare workers, healthcare personnel, from PubMed, ScienceDirect, Embase, Scopus, Web of Sciences, and Google Scholar. Data were descriptively analyzed. The insistence on hand washing has a history of 1400 years. The research results indicate that the bacteria released from the female washed hands in wet and dry condition was lower than from the male's hands with a significance level (3 CFU vs. 8 CFU; confidence interval 95%, P ≤ 0.001). The valuable results of the study indicated that released amount of bacterial flora from wet hands is more than 10 times in compared to dry hands. In addition, established monitoring systems for washing hands before and after patient's manipulation as well as after toilet were dominant indices to prevent the transfer of infectious agents to the patients. Increasing awareness and belief of the healthcare workers have shown an important role by about 30% reduction in the transfection. Hand washing could reduce the episodes of transmission of infectious agents in both community and healthcare settings. However, hand washing is an important key factor to prevent transmission of infectious agents to patients. There is no standard method for measuring compliance. Thus, permanent monitoring of hand washing to reduce the transmission of infections is crucial. Finally, the personnel must believe that hand washing is an inevitable approach to infection control. PMID:28382192

  14. The effect of improving cow productivity, fertility, and longevity on the global warming potential of dairy systems.

    PubMed

    Bell, M J; Wall, E; Russell, G; Simm, G; Stott, A W

    2011-07-01

    This study compared the environmental impact of a range of dairy production systems in terms of their global warming potential (GWP, expressed as carbon dioxide equivalents, CO(2)-eq.) and associated land use, and explored the efficacy of reducing said impact. Models were developed using the unique data generated from a long-term genetic line × feeding system experiment. Holstein-Friesian cows were selected to represent the UK average for milk fat plus protein production (control line) or were selected for increased milk fat plus protein production (select line). In addition, cows received a low forage diet (50% forage) with no grazing or were on a high forage (75% forage) diet with summer grazing. A Markov chain approach was used to describe the herd structure and help estimate the GWP per year and land required per cow for the 4 alternative systems and the herd average using a partial life cycle assessment. The CO(2)-eq. emissions were expressed per kilogram of energy-corrected milk (ECM) and per hectare of land use, as well as land required per kilogram of ECM. The effects of a phenotypic and genetic standard deviation unit improvement on herd feed utilization efficiency, ECM yield, calving interval length, and incidence of involuntary culling were assessed. The low forage (nongrazing) feeding system with select cows produced the lowest CO(2)-eq. emissions of 1.1 kg/kg of ECM and land use of 0.65 m(2)/kg of ECM but the highest CO(2)-eq. emissions of 16.1t/ha of the production systems studied. Within the herd, an improvement of 1 standard deviation in feed utilization efficiency was the only trait of those studied that would significantly reduce the reliance of the farming system on bought-in synthetic fertilizer and concentrate feed, as well as reduce the average CO(2)-eq. emissions and land use of the herd (both by about 6.5%, of which about 4% would be achievable through selective breeding). Within production systems, reductions in CO(2)-eq. emissions per

  15. Matching forage systems with cow size and environment for sustainable cow-calf production in the southern region

    USDA-ARS?s Scientific Manuscript database

    There has been increased interest in intensification of cow-calf production due to an increasing world population and red meat demand along with reductions in available grazing lands. Intensified production can come about by increasing fertilization, supplementation, or feeding of stored forages, bu...

  16. A deterministic, dynamic systems model of cow-calf production: The effects of the duration of postpartum anestrus on production parameters over a 10-year horizon.

    PubMed

    Shane, D D; Larson, R L; Sanderson, M W; Miesner, M; White, B J

    2017-04-01

    The duration of postpartum anestrus (dPPA) is important to consider for reproductive performance and efficiency in cow-calf operations. We developed a deterministic, dynamic systems model of cow-calf production over a 10-yr horizon to model the effects that dPPA had on measures of herd productivity, including the percentage of cows cycling before the end of the first 21 d of the breeding season (%C21), the percentage of cows pregnant at pregnancy diagnosis (%PPD), the distribution of pregnancy by 21-d breeding intervals, the kilograms of calf weaned (KW), the kilograms of calf weaned per cow exposed (KPC), and the replacement percentage. A 1,000-animal herd was modeled, with the beginning and ending dates for a 63-d natural service breeding season being the same for eligible replacement heifers (nulliparous cows) and cows (primiparous and multiparous cows). Herds were simulated to have a multiparous cow dPPA of 50, 60, 70, or 80 d, with the dPPA for primiparous cows being set to 50, 60, 70, 80, 90, 100, or 110 d. Only combinations where the primiparous dPPA was greater than or equal to the multiparous dPPA were included, resulting in 22 model herds being simulated in the analysis. All other model parameters were held constant between simulations. In model season 10, the %C21 was 96.2% when the multiparous cow and primiparous cow dPPA was 50 d and was 48.3% when the multiparous cow and primiparous cow dPPA was 80 d. The %PPD in model season 10 for these same herds was 95.1% and 86.0%, respectively. The percentage of the herd becoming pregnant in the first 21 d of the breeding season also differed between these herds (61.8% and 31.3%, respectively). The 10-yr total KW was more than 275,000 kg greater for the herd with a 50-d multiparous cow and primiparous cow dPPA when compared with the herd with the 80-d multiparous and primiparous cow dPPA and had a model season 10 KPC of 180.8 kg compared with 151.4 kg for the longer dPPA. The model results show that both the

  17. 30 CFR 206.458 - Determination of washing allowances.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MANAGEMENT PRODUCT VALUATION Indian Coal § 206.458 Determination of washing allowances. (a) Arm's-length... allowance shall be the reasonable actual costs incurred by the lessee for washing the coal under that... deduction may be taken, the lessee must submit a completed page one of Form MMS-4292, Coal Washing Allowance...

  18. Association of standing and lying behavior patterns and incidence of intramammary infection in dairy cows milked with an automatic milking system.

    PubMed

    Devries, T J; Deming, J A; Rodenburg, J; Seguin, G; Leslie, K E; Barkema, H W

    2011-08-01

    The standing and lying behavior patterns of dairy cows, particularly the length of time cows spend standing after milking, have the potential to influence the incidence of intramammary infection (IMI). The objectives were to describe the standing and lying behavior patterns of cows milked with an automatic milking system (AMS) and to determine how these patterns relate to the incidence of IMI. One hundred and eleven lactating Holstein dairy cows were monitored over a 4-mo period. These cows were kept in a sand-bedded freestall barn with 2 pens, each with a free cow traffic AMS. Feed was delivered once daily, and pushed up 2 to 3 times daily. Quarter milk samples were collected for bacteriological culture from each cow once every 4 wk. A new IMI was defined as a positive culture sample following a negative culture. For 7 d before each of the last 3 milk samplings, standing and lying behavior, and times of milking and feed manipulation (feed delivery and push up) were recorded. Daily lying time and lying bout length were negatively related with milk yield (r=-0.23 and -0.20, respectively) and milking frequency (r=-0.32 and -0.20, respectively); milk yield was positively related to milking frequency (r=0.58). Feed manipulation near the time cows were milked (1h before 2h after) resulted in the longest post-milking standing times (mean=86 min; 95% confidence interval=78, 94 min), whereas feed manipulation occurring outside that time frame resulted in shorter post-milking standing times. Over the study period, 171 new IMI were detected. Of these new IMI detected, those caused by coagulase-negative staphylococci were the only ones associated with post-milking standing time; as post-milking standing time increased past 2.5h after milking, the odds of acquiring a new IMI tended to also increase. In summary, standing and lying behavior patterns of cows milked with an AMS were affected by both feed manipulation and their milking activity. Further, the post-milking standing

  19. 33 CFR 157.138 - Crude Oil Washing Operations and Equipment Manual.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) Revolutions, number of cycles, and length of cycles of each COW machine. (iii) Pressure and flow of the... COW machines. (ii) Revolutions, number of cycles, and length of cycles of each COW machine. (iii... § 157.140. (10) The volume of water used for water rinsing recorded during COW operations when passing...

  20. 33 CFR 157.138 - Crude Oil Washing Operations and Equipment Manual.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) Revolutions, number of cycles, and length of cycles of each COW machine. (iii) Pressure and flow of the... COW machines. (ii) Revolutions, number of cycles, and length of cycles of each COW machine. (iii... § 157.140. (10) The volume of water used for water rinsing recorded during COW operations when passing...

  1. 33 CFR 157.138 - Crude Oil Washing Operations and Equipment Manual.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) Revolutions, number of cycles, and length of cycles of each COW machine. (iii) Pressure and flow of the... COW machines. (ii) Revolutions, number of cycles, and length of cycles of each COW machine. (iii... § 157.140. (10) The volume of water used for water rinsing recorded during COW operations when passing...

  2. Position document: IgE-mediated cow's milk allergy.

    PubMed

    Martorell-Aragonés, A; Echeverría-Zudaire, L; Alonso-Lebrero, E; Boné-Calvo, J; Martín-Muñoz, M F; Nevot-Falcó, S; Piquer-Gibert, M; Valdesoiro-Navarrete, L

    2015-01-01

    The present document offers an update on the recommendations for managing patients with cow's milk allergy - a disorder that manifests in the first year of life, with an estimated prevalence of 1.6-3% in this paediatric age group. The main causal allergens are the caseins and proteins in lactoserum (beta-lactoglobulin, alpha-lactoalbumin), and the clinical manifestations are highly variable in terms of their presentation and severity. Most allergic reactions affect the skin, followed by the gastrointestinal and respiratory systems, and severe anaphylaxis may occur. The diagnosis of cow's milk allergy is based on the existence of a suggestive clinical history, a positive allergy study and the subsequent application of controlled exposure testing, which constitutes the gold standard for confirming the diagnosis. The most efficient treatment for cow's milk allergy is an elimination diet and the use of adequate substitution formulas. The elimination diet must include milk from other mammals (e.g., sheep, goat, etc.) due to the risk of cross-reactivity with the proteins of cow's milk. Most infants with IgE-mediated cow's milk allergy become tolerant in the first few years of life. In those cases where cow's milk allergy persists, novel treatment options may include oral immunotherapy, although most authors do not currently recommend this technique in routine clinical practice. Enough evidence is not there to confirm the efficacy of elimination diets in the mother and infant for preventing the appearance of cow's milk allergy. Likewise, no benefits have been observed with prebiotic and probiotic dietetic supplements in infants for preventing food allergy. Copyright © 2015 SEICAP. Published by Elsevier Espana. All rights reserved.

  3. Effect of chromium on bioenergetics and leukocyte dynamics following immunoactivation in lactating Holstein cows.

    PubMed

    Horst, E A; Kvidera, S K; Mayorga, E J; Shouse, C S; Al-Qaisi, M; Dickson, M J; Ydstie, J; Ramirez Ramirez, H A; Keating, A F; Dickson, D J; Griswold, K E; Baumgard, L H

    2018-06-01

    Activated immune cells are insulin sensitive and utilize copious amounts of glucose. Because chromium (Cr) increases insulin sensitivity and may be immunomodulatory, our objective was to evaluate the effect of supplemental Cr (KemTrace Cr propionate, 20 g/d; Kemin Industries Inc., Des Moines, IA) on immune system glucose utilization and immune system dynamics following an intravenous endotoxin challenge in lactating Holstein cows. Twenty cows (320 ± 18 d in milk) were randomly assigned to 1 of 4 treatments: (1) pair-fed (PF) control (PF-CON; 5 mL of saline; n = 5), (2) PF and Cr supplemented (PF-Cr; 5 mL of saline; n = 5), (3) lipopolysaccharide (LPS)-euglycemic clamp and control supplemented (LPS-CON; 0.375 µg/kg of body weight LPS; n = 5), and (4) LPS-euglycemic clamp and Cr supplemented (LPS-Cr; 0.375 µg/kg of body weight LPS; n = 5). The experiment was conducted serially in 3 periods (P). During P1 (3 d), cows received their respective dietary treatments and baseline values were obtained. At the initiation of P2 (2 d), either a 12-h LPS-euglycemic clamp was conducted or cows were PF to their respective dietary counterparts. During P3 (3 d), cows consumed feed ad libitum and continued to receive their respective dietary treatment. During P2, LPS administration decreased dry matter intake (DMI; 40%) similarly among diets, and by experimental design the pattern and magnitude of reduced DMI were similar in the PF cohorts. During P3, LPS-Cr cows tended to have decreased DMI (6%) relative to LPS-CON cows. Relative to controls, milk yield from LPS-challenged cows decreased (58%) during P2 and LPS-Cr cows produced less (16%) milk than LPS-CON cows. During P3, milk yield progressively increased similarly in LPS-administered cows, but overall milk yield remained decreased (24%) compared with PF controls. There were no dietary treatment differences in milk yield during P3. Circulating insulin increased 9- and 15-fold in LPS-administered cows at 6 and 12 h postbolus

  4. Performance of ceramic ultrafiltration and reverse osmosis membranes in treating car wash wastewater for reuse.

    PubMed

    Moazzem, Shamima; Wills, Jamie; Fan, Linhua; Roddick, Felicity; Jegatheesan, Veeriah

    2018-03-01

    Reusing treated effluents in industries is a great option to conserve freshwater resources. For example, car wash centres all over Australia are estimated to use 17.5 billion litres of water and discharge it as wastewater and spend $75 million a year for both purchasing fresh water and for treating and/or discharging the wastewater. Therefore, it is important to develop simple but reliable systems that can help to treat and reuse car wash wastewater. Significant savings could also be associated with the implementation of such systems. This study evaluates the performance of granular and membrane filtration systems with coagulation/flocculation and sedimentation in treating car wash wastewater for the purpose of reuse. Overall, 99.9% of turbidity, 100% of suspended solids and 96% of COD were removed from the car wash wastewater after treating by coagulation, flocculation, sedimentation, sand filtration, ceramic ultrafiltration and reverse osmosis and the treated water meets the standards required for class A recycled water in Australia and standards imposed in Belgium and China. The treated water can be reused. However, optimisation is required to reduce the sludge produced by this system.

  5. Hand washing promotion for preventing diarrhoea.

    PubMed

    Ejemot-Nwadiaro, Regina I; Ehiri, John E; Arikpo, Dachi; Meremikwu, Martin M; Critchley, Julia A

    2015-09-03

    Diarrhoea accounts for 1.8 million deaths in children in low- and middle-income countries (LMICs). One of the identified strategies to prevent diarrhoea is hand washing. To assess the effects of hand washing promotion interventions on diarrhoeal episodes in children and adults. We searched the Cochrane Infectious Diseases Group Specialized Register (27 May 2015); CENTRAL (published in the Cochrane Library 2015, Issue 5); MEDLINE (1966 to 27 May 2015); EMBASE (1974 to 27 May 2015); LILACS (1982 to 27 May 2015); PsycINFO (1967 to 27 May 2015); Science Citation Index and Social Science Citation Index (1981 to 27 May 2015); ERIC (1966 to 27 May 2015); SPECTR (2000 to 27 May 2015); Bibliomap (1990 to 27 May 2015); RoRe, The Grey Literature (2002 to 27 May 2015); World Health Organization (WHO) International Clinical Trial Registry Platform (ICTRP), metaRegister of Controlled Trials (mRCT), and reference lists of articles up to 27 May 2015. We also contacted researchers and organizations in the field. Individually randomized controlled trials (RCTs) and cluster-RCTs that compared the effects of hand washing interventions on diarrhoea episodes in children and adults with no intervention. Three review authors independently assessed trial eligibility, extracted data, and assessed risk of bias. We stratified the analyses for child day-care centres or schools, community, and hospital-based settings. Where appropriate, incidence rate ratios (IRR) were pooled using the generic inverse variance method and random-effects model with 95% confidence intervals (CIs). We used the GRADE approach to assess the quality of evidence. We included 22 RCTs: 12 trials from child day-care centres or schools in mainly high-income countries (54,006 participants), nine community-based trials in LMICs (15,303 participants), and one hospital-based trial among people with acquired immune deficiency syndrome (AIDS) (148 participants).Hand washing promotion (education activities, sometimes with

  6. Saline nasal washes

    MedlinePlus

    ... nasal wash helps flush pollen, dust, and other debris from your nasal passages. It also helps remove excess mucus (snot) and adds moisture. Your nasal passages are open spaces behind your nose. Air passes through your nasal ...

  7. Beef cow-calf production.

    PubMed

    Feuz, Dillon M; Umberger, Wendy J

    2003-07-01

    Cow-calf production occurs in all 50 states over varied resource bases and under vastly different environmental conditions. Multiple breeds exist and management styles and objectives are as numerous as the number of cow-calf producers. There is not one area of the country, one breed of cattle, or one management style that is most profitable for producing cows and calves. There are, however, some common strategies that can be employed by cow-calf producers to enhance profitability. Costs need to be controlled without jeopardizing cow herd productivity or net returns. It appears that the cost associated with purchased and harvested feeds varies considerably across operations. Understanding cyclic and seasonal price patterns, weight-price slides, cattle shrink, and other marketing costs can help producers enhance their profit by marketing (and not by just selling) their cattle. Producers with superior cattle genetics can become part of a specific alliance or, at a minimum, document the performance of their cattle so that they can get paid for the superior genetics. The beef industry is changing and will likely continue to change. Cow-calf producers will need to examine their own management practices to determine whether they are optimal for the current industry. Those producers who are most adept at matching their management abilities to their cattle type, their resource base, and the appropriate market outlet will be the most successful in the future.

  8. ANAEROBIC TREATMENT OF SOIL WASH FLUIDS FROM A WOOD PRESERVING SITE

    EPA Science Inventory

    An integrated system has been developed to remediate sols contaminated with pentachlorophenol (PCP) and polycyclic aromatic hydrocarbons (PAHs). This system involves the coupling of two treatment technologies, soil solvent washing and anaerobic biotreatment of the extract. Specif...

  9. Cow's milk and goat's milk.

    PubMed

    Turck, Dominique

    2013-01-01

    Cow's milk is increasingly suggested to play a role in the development of chronic degenerative, non-communicable disorders whereas goat's milk is advocated as having several health benefits. Cow's milk is a rich and cheap source of protein and calcium, and a valuable food for bone health. Despite their high content in saturated fats, consumption of full-fat dairy products does not seem to cause significant changes in cardiovascular disease risk variables. Early introduction of cow's milk is a strong negative determinant of iron status. Unmodified cow's milk does not meet nutritional requirements of infants although it is acceptable to add small volumes of cow's milk to complementary foods. Cow's milk protein allergy has a prevalence ranging from 2 to 7%, and the age of recovery is usually around 2-3 years. The evidence linking cow's milk intake to a later risk of type 1 diabetes or chronic degenerative, non-communicable disorders (obesity, metabolic syndrome, type 2 diabetes, hypertension) is not convincing. Milk probably protects against colorectal cancer, diets high in calcium are a probable cause of prostate cancer, and there is limited evidence suggesting that high consumption of milk and dairy products increases the risk for prostate cancer. There is no evidence to support the use of a cow's milk-free diet as a primary treatment for individuals with autistic spectrum disorders. Unmodified goat's milk is not suitable for infants because of the high protein and minerals content and of a low folate content. Goat's milk has no clear nutritional advantage over cow's milk and is not less allergenic. The European Food Safety Authority recently stated that proteins from goat's milk can be suitable as a protein source for infant and follow-on formula, provided the final product complies with the compositional criteria laid down in Directive 2006/141/EC. Copyright © 2013 S. Karger AG, Basel.

  10. [Automated detection of estrus and mastitis in dairy cows].

    PubMed

    de Mol, R M

    2001-02-15

    The development and test of detection models for oestrus and mastitis in dairy cows is described in a PhD thesis that was defended in Wageningen on June 5, 2000. These models were based on sensors for milk yield, milk temperature, electrical conductivity of milk, and cow activity and concentrate intake, and on combined processing of the sensor data. The models alert farmers to cows that need attention, because of possible oestrus or mastitis. A first detection model for cows, milked twice a day, was based on time series models for the sensor variables. A time series model describes the dependence between successive observations. The parameters of the time series models were fitted on-line for each cow after each milking by means of a Kalman filter, a mathematical method to estimate the state of a system on-line. The Kalman filter gives the best estimate of the current state of a system based on all preceding observations. This model was tested for 2 years on two experimental farms, and under field conditions on four farms over several years. A second detection model, for cow milked in an automatic milking system (AMS), was based on a generalization of the first model. Two data sets (one small, one large) were used for testing. The results for oestrus detection were good for both models. The results for mastitis detection were varying (in some cases good, in other cases moderate). Fuzzy logic was used to classify mastitis and oestrus alerts with both detection models, to reduce the number of false positive alerts. Fuzzy logic makes approximate reasoning possible, where statements can be partly true or false. Input for the fuzzy logic model were alerts from the detection models and additional information. The number of false positive alerts decreased considerably, while the number of detected cases remained at the same level. These models make automated detection possible in practice.

  11. [Kinetics of heifers and cows walking on an instrumented treadmill].

    PubMed

    Nuss, K; Waldern, N M; Weishaupt, M A; Wiestner, T

    2015-01-01

    Kinetic data of stride characteristics and ground reaction forces of cattle become increasingly important as automated lameness detection may be installed in dairy cow housing systems in the future. Therefore, sound heifers and cows were measured on an instrumented treadmill to collect such basic data. Nine heifers and 10 cows were trained to walk on an instrumented treadmill. Vertical ground reaction forces as well as step and stride timing and length variables were measured for all limbs simultaneously. On average, 16 stride cycles in cows and 24 strides in heifers were analysed in each case. The cows walked on the treadmill at an average speed of 1.2 ± 0.05 m/s (mean ± standard deviation), with a stride rate of 43.0 ± 1.9/min and a stride length of 1.68 ± 0.1 m. The heifers had average values of 1.3 ± 0.04 m/s, 53.7 ± 2.2/min and 1.49 ± 0.05 m, respectively. The stance duration relative to stride duration (the duty factor) was for the cows significantly longer in the forelimbs (67%) than in the hind limbs (64%). Force-time-curves of all limbs showed two peaks, one after landing (FP1) and another during push off (FP2). Vertical ground reaction force was highest for FP1 in the hind limbs, but for FP2 in the forelimbs. At all limbs, force minimum between the peaks occurred shortly before midstance. The vertical impulse carried by both forelimbs amounted to 53.7% of the total stride impulse in cows and to 55.0% in heifers. The location of the centre of body mass varied during the stride cycle but was always located more towards the front limbs. Cows and heifers showed a symmetrical walk with minimal intra-individual variations. Relative stride impulse of the front limbs was higher than that of the hind limbs. Peak vertical force in the hind limbs was highest at landing and in the forelimbs at push off. The present study offers kinetic data of sound cows and heifers which might be helpful as guidelines for automated systems for lameness detection in cattle.

  12. Effect of diagnosis and treatment of clinical endometritis based on vaginal discharge score grading system in postpartum Holstein cows

    PubMed Central

    OKAWA, Hiroaki; FUJIKURA, Atsushi; WIJAYAGUNAWARDANE, Missaka M.P.; VOS, Peter L.A.M.; TANIGUCHI, Masayasu; TAKAGI, Mitsuhiro

    2017-01-01

    In this study, the prevalence, effectiveness of diagnosis, and treatment based on vaginal discharge score (VDS) of clinical endometritis in cattle were evaluated. To detect clinical endometritis and classify its severity, vaginoscopy was performed during 21 to 60 days postpartum in 164 Holstein cows consisting of 229 lactations. Groups were defined using the 4-point VDS scale. Study groups included the following: non-endometritis (VDS=0; no/clear mucus; NEM group; n=168); mild endometritis, no treatment (VDS=1; mucus containing flecks of white/off-white pus; NTR group; n=30); and severe endometritis, treated with PGF2α (VDS≥2; discharge containing <50% pus; and VDS=3; discharge containing >50% pus, and fluid or uterine horn asymmetry; TEM group; n=31). Cows treated with PGF2α that did not recover (VDS≥1, n=5) received intrauterine procaine penicillin and streptomycin. Prevalence of clinical endometritis (VDS≥1) was 26.6%. The NTR group required significantly more artificial inseminations per pregnancy than NEM and TEM groups (2.8 ± 1.8 vs 2.0 ± 1.3, 1.9 ± 0.8, P<0.05). In survival analysis, the proportion of non-pregnant cows was higher in the NTR group compared to the NEM (P=0.012) and TEM (P=0.076) groups. In the TEM group, calving to first artificial insemination interval tended to be higher in cows treated 41 to 60 days postpartum than cows treated 29 to 40 days postpartum (97.2 ± 27.1 vs 74.4 ± 19.7, P=0.084). Our study suggests that cows with VDS=1 may require treatment to recover fertility. Diagnosis and treatment of clinical endometritis based on a VDS grading system may improve dairy herd reproductive performance. PMID:28740032

  13. Effect of diagnosis and treatment of clinical endometritis based on vaginal discharge score grading system in postpartum Holstein cows.

    PubMed

    Okawa, Hiroaki; Fujikura, Atsushi; Wijayagunawardane, Missaka M P; Vos, Peter L A M; Taniguchi, Masayasu; Takagi, Mitsuhiro

    2017-09-12

    In this study, the prevalence, effectiveness of diagnosis, and treatment based on vaginal discharge score (VDS) of clinical endometritis in cattle were evaluated. To detect clinical endometritis and classify its severity, vaginoscopy was performed during 21 to 60 days postpartum in 164 Holstein cows consisting of 229 lactations. Groups were defined using the 4-point VDS scale. Study groups included the following: non-endometritis (VDS=0; no/clear mucus; NEM group; n=168); mild endometritis, no treatment (VDS=1; mucus containing flecks of white/off-white pus; NTR group; n=30); and severe endometritis, treated with PGF2α (VDS≥2; discharge containing <50% pus; and VDS=3; discharge containing >50% pus, and fluid or uterine horn asymmetry; TEM group; n=31). Cows treated with PGF2α that did not recover (VDS≥1, n=5) received intrauterine procaine penicillin and streptomycin. Prevalence of clinical endometritis (VDS≥1) was 26.6%. The NTR group required significantly more artificial inseminations per pregnancy than NEM and TEM groups (2.8 ± 1.8 vs 2.0 ± 1.3, 1.9 ± 0.8, P<0.05). In survival analysis, the proportion of non-pregnant cows was higher in the NTR group compared to the NEM (P=0.012) and TEM (P=0.076) groups. In the TEM group, calving to first artificial insemination interval tended to be higher in cows treated 41 to 60 days postpartum than cows treated 29 to 40 days postpartum (97.2 ± 27.1 vs 74.4 ± 19.7, P=0.084). Our study suggests that cows with VDS=1 may require treatment to recover fertility. Diagnosis and treatment of clinical endometritis based on a VDS grading system may improve dairy herd reproductive performance.

  14. Effect of concentrate feeding method on the performance of dairy cows in early to mid lactation.

    PubMed

    Purcell, P J; Law, R A; Gordon, A W; McGettrick, S A; Ferris, C P

    2016-04-01

    The objective of the current study was to determine the effects of concentrate feeding method on milk yield and composition, dry matter (DM) intake (DMI), body weight and body condition score, reproductive performance, energy balance, and blood metabolites of housed (i.e., accommodated indoors) dairy cows in early to mid lactation. Eighty-eight multiparous Holstein-Friesian cows were managed on 1 of 4 concentrate feeding methods (CFM; 22 cows per CFM) for the first 21 wk postpartum. Cows on all 4 CFM were offered grass silage plus maize silage (in a 70:30 ratio on a DM basis) ad libitum throughout the study. In addition, cows had a target concentrate allocation of 11 kg/cow per day (from d 13 postpartum) via 1 of 4 CFM, consisting of (1) offered on a flat-rate basis via an out-of-parlor feeding system, (2) offered based on individual cow's milk yields in early lactation via an out-of-parlor feeding system, (3) offered as part of a partial mixed ration (target intake of 5 kg/cow per day) with additional concentrate offered based on individual cow's milk yields in early lactation via an out-of-parlor feeding system, and (4) offered as part of a partial mixed ration containing a fixed quantity of concentrate for each cow in the group. In addition, all cows were offered 1 kg/cow per day of concentrate pellets via an in-parlor feeding system. We detected no effect of CFM on concentrate or total DMI, mean daily milk yield, concentrations and yields of milk fat and protein, or metabolizable energy intakes, requirements, or balances throughout the study. We also found no effects of CFM on mean or final body weight, mean or final body condition score, conception rates to first service, or any of the blood metabolites examined. The results of this study suggest that CFM has little effect on the overall performance of higher-yielding dairy cows in early to mid lactation when offered diets based on conserved forages. Copyright © 2016 American Dairy Science Association

  15. View of Steel Flume Bridge #2 crossing over wash. Looking ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View of Steel Flume Bridge #2 crossing over wash. Looking downstream, southwest - Childs-Irving Hydroelectric Project, Childs System, Flume Bridge No. 2, Forest Service Road 708/502, Camp Verde, Yavapai County, AZ

  16. Percutaneous penetration and pharmacodynamics: Wash-in and wash-off of sunscreen and insect repellent.

    PubMed

    Rodriguez, Jocelyn; Maibach, Howard I

    2016-01-01

    Increased awareness of skin cancer and mosquito-transmitted diseases has increased use of insect repellents and sunscreens. The challenge in setting recommendations for use and reapplication, especially when used concomitantly, lies in finding the balance between applying a durable product effective in withstanding natural and physical factors such as water, sweat, temperature and abrasion, while limiting percutaneous absorption and decreasing risk of potential dermal and systemic toxicity. Inorganic sunscreens show no or little percutaneous absorption or toxic effects in comparison to organic sunscreens, which show varying levels of dermal penetration and cutaneous adverse effects. An alternative to N,N-diethyl-m-toluamide (DEET), the traditional gold standard compound in insect repellents, picaridin appears as efficacious, has lower risk of toxicity, and when used simultaneously with sunscreen may decrease percutaneous absorption of both compounds. Conversely, combined use of DEET and sunscreen results in significantly higher absorption of both compounds. It is important to increase consumer awareness of "washing in" of various compounds leading to increased risk of toxicity, as well as differences in reapplication need due to "washing off" caused by water, sweat and abrasion. Although much remains to be studied, to maximize efficacy and decrease toxicity, contemporary research tools, including dermatopharmokinetics, should aid these prospective advances.

  17. A Case Study of Behaviour and Performance of Confined or Pastured Cows During the Dry Period

    PubMed Central

    Black, Randi A.; Krawczel, Peter D.

    2016-01-01

    Simple Summary Pasture and freestall systems offer benefits and consequences during lactation but have not been investigated during the dry period. The effect of pasture or confined systems during the dry period on behaviour and milk quality was investigated. Freestall housing resulted in more resting behaviour and less locomotor activity during the dry period compared to pastured cows. At calving, freestall housed cows performed fewer lying bouts and less locomotor activity compared to pastured cows. Pasture resulted in less aggression around feeding but high respiration rates during peak heat times. Pasture during the dry period altered lying behavior, reduced feed bunk aggression and increased heat stress behaviors. Abstract The objectives of this study were to determine the effect of the dry cow management system (pasture or confined) on: (1) lying behaviour and activity; (2) feeding and heat stress behaviours; (3) intramammary infections, postpartum. Non-lactating Holstein cows were assigned to either deep-bedded, sand freestalls (n = 14) or pasture (n = 14) using rolling enrollment. At dry-off, cows were equipped with an accelerometer to determine daily lying time (h/d), lying bouts (bouts/d), steps (steps/d) and divided into periods: far-off (60 to 15 d prepartum), close-up (14 to 1 d prepartum), calving (calving date) and postpartum (1 to 14 d postpartum). Respiration rates were recorded once weekly from dry off to calving from 1300 to 1500 h. Feeding displacements were defined as one cow successfully displacing another from the feed bunk and were recorded once per week during the 2 h period, immediately after feeding at 800 h. Pastured cows were fed a commercial dry cow pellet during far-off and total mixed ration during close-up, with free access to hay and grazing. Freestall housed cows were fed a total mixed ration at far-off and close-up. Cows housed in freestalls were moved to a maternity pen with a mattress at commencement of labour. Pastured cows

  18. Delaying postpartum supplementation in cows consuming low-quality forage does not alter cow and calf productivity

    USDA-ARS?s Scientific Manuscript database

    Reducing the amount of supplemental feed postpartum without affecting productivity may enhance profitability of cow-calf operations. Therefore, sixteen 2-yr-old fall calving cows were used to evaluate effects of delaying postpartum supplementation on milk production, serum metabolites, cow and calf ...

  19. A comparison of individual cow versus group concentrate allocation strategies on dry matter intake, milk production, tissue changes, and fertility of Holstein-Friesian cows offered a grass silage diet.

    PubMed

    Little, M W; O'Connell, N E; Ferris, C P

    2016-06-01

    A diverse range of concentrate allocation strategies are adopted on dairy farms. The objectives of this study were to examine the effects on cow performance [dry matter (DM) intake (DMI), milk yield and composition, body tissue changes, and fertility] of adopting 2 contrasting concentrate allocation strategies over the first 140 d of lactation. Seventy-seven Holstein-Friesian dairy cows were allocated to 1 of 2 concentrate allocation strategies at calving, namely group or individual cow. Cows on the group strategy were offered a mixed ration comprising grass silage and concentrates in a 50:50 ratio on a DM basis. Cows on the individual cow strategy were offered a basal mixed ration comprising grass silage and concentrates (the latter included in the mix to achieve a mean intake of 6kg/cow per day), which was formulated to meet the cow's energy requirements for maintenance plus 24kg of milk/cow per day. Additional concentrates were offered via an out-of-parlor feeding system, with the amount offered adjusted weekly based on each individual cow's milk yield during the previous week. In addition, all cows received a small quantity of straw in the mixed ration part of the diet (approximately 0.3kg/cow per day), plus 0.5kg of concentrate twice daily in the milking parlor. Mean concentrate intakes over the study period were similar with each of the 2 allocation strategies (11.5 and 11.7kg of DM/cow per day for group and individual cow, respectively), although the pattern of intake with each treatment differed over time. Concentrate allocation strategy had no effect on either milk yield (39.3 and 38.0kg/d for group and individual cow, respectively), milk composition, or milk constituent yield. The milk yield response curves with each treatment were largely aligned with the concentrate DMI curves. Cows on the individual cow treatment had a greater range of concentrate DMI and milk yields than those on the group treatment. With the exception of a tendency for cows on the

  20. Risk factors for lameness in freestall-housed dairy cows across two breeds, farming systems, and countries.

    PubMed

    Dippel, S; Dolezal, M; Brenninkmeyer, C; Brinkmann, J; March, S; Knierim, U; Winckler, C

    2009-11-01

    Lameness poses a considerable problem in modern dairy farming. Several new developments (e.g., herd health plans) strive to help farmers improve the health and welfare of their herd. It was thus our aim to identify lameness risk factors common across regions, breeds, and farming systems for freestall-housed dairy cows. We analyzed data from 103 nonorganic and organic dairy farms in Germany and Austria that kept 24 to 145 Holstein Friesian or Fleckvieh cows in the milking herd (mean = 48). Data on housing, management, behavior, and lameness scores for a total of 3,514 cows were collected through direct observations and an interview. Mean lameness prevalence was 34% (range = 0-81%). Data were analyzed applying logistic regression with generalized estimating equations in a split-sample design. The final model contained 1 animal-based parameter and 3 risk factors related to lying as well as 1 nutritional animal-based parameter, while correcting for the significant confounders parity and data subset. Risk for lameness increased with decreasing lying comfort, that is, more frequent abnormal lying behavior, mats or mattresses used as a stall base compared with deep-bedded stall bases, the presence of head lunge impediments, or neck rail-curb diagonals that were too short. Cows in the lowest body condition quartile (1.25-2.50 for Holstein Friesian and 2.50-3.50 for Fleckvieh) had the highest risk of being lame. In cross-validation the model correctly classified 71 and 70% of observations in the model-building and validation samples, respectively. Only 2 out of 15 significant odds ratios (including contrasts) changed direction. They pertained to the 2 variables with the highest P-values in the model. In conclusion, lying comfort and nutrition are key risk areas for lameness in freestall-housed dairy cows. Abnormal lying behavior in particular proved to be a good predictor of lameness risk and should thus be included in on-farm protocols. The study is part of the European

  1. Separate housing for one month after calving improves production and health in primiparous cows but not in multiparous cows.

    PubMed

    Østergaard, S; Thomsen, P T; Burow, E

    2010-08-01

    The hypothesis was that dairy cows housed for 1 mo after calving in a separate group with herd mates would produce more milk and would be healthier than cows integrated in a group of all lactating cows immediately after calving. The experiment was conducted with 489 cows in 6 commercial loose-housing dairy herds where cows were randomly selected for treatment (separate housing) or control. Cows selected for treatment were housed for 1 mo after calving in a separate section, and controls were housed in the remaining section of the barn for lactating cows. Data were compared for milk yield, somatic cell count, medical treatments, reproductive performance, culling, mortality, and clinical observation of scores for body condition, leg and udder hygiene, lameness, hock lesions, other cutaneous lesions, vaginal discharge, and condition of the hair coat. The analysis of the effect of separate housing showed that primiparous cows produced more milk [approximately 230 kg of energy-corrected milk from 0 to 305 d in milk (DIM)], whereas multiparous and especially parity 3+ cows produced less milk (approximately 394 kg of energy-corrected milk from 0 to 305 DIM) during the lactation. Separate housing had no effect on mortality or reproductive efficiency. In primiparous cows, the number of medical treatments for ketosis was reduced by separate housing [hazard ratio 0.33, 95% confidence interval (CI): 0.13-0.83]. Clinical evaluations showed that separate housing decreased the scores for hock lesions in cows at 0 to 30 DIM (odds ratio 0.41, CI: 0.19-0.91), whereas the scoring of leg cleanliness showed more dirty legs in separated cows at 0 to 30 DIM (odds ratio 3.61, CI: 2.01-6.47) compared with cows integrated into the herd immediately. The body condition score in separated cows was reduced from 0 to 30 DIM (score reduced by 0.16, CI: 0.07-0.25) and from 31 to 60 DIM (score reduced by 0.13, CI: 0.04-0.23) compared with cows integrated immediately. We concluded that separate

  2. Designing Better Water Troughs: Does Trough Color Influence Dairy Cows' Preference?

    PubMed

    Lemos Teixeira, Dayane; Hötzel, Maria José; Pinheiro Machado Filho, Luiz Carlos; Cazale, José Daniel; Enríquez-Hidalgo, Daniel

    2017-01-01

    Eighteen lactating dairy cows were used to elucidate their preference for green, grey, or red troughs. The herd was managed under a rotational grazing system with ad-libitum access to water until 11:30 h. For 9 days, all cows were tested individually following the afternoon milking. Cows drank similar quantities, spent a similar amount of time drinking, and took a similar number of sips from the 3 trough colors (p > .05). In 75% of the tests, cows drank more than 95% of the test period from the same trough. Within this time, the percentage of choices did not differ among colors (33.3% green, 39.0% grey, and 27.7% red). When they chose the red trough, cows spent less time drinking (p ≤ .05) and tended to take fewer sips (p = .07), which could suggest a partial aversion to this color. Suboptimal water trough design may have long-term negative effects on both the production and welfare of dairy cattle; however, the results suggest that color does not play a major role in the drinking behavior of dairy cows.

  3. Release of synthetic microplastic plastic fibres from domestic washing machines: Effects of fabric type and washing conditions.

    PubMed

    Napper, Imogen E; Thompson, Richard C

    2016-11-15

    Washing clothes made from synthetic materials has been identified as a potentially important source of microscopic fibres to the environment. This study examined the release of fibres from polyester, polyester-cotton blend and acrylic fabrics. These fabrics were laundered under various conditions of temperature, detergent and conditioner. Fibres from waste effluent were examined and the mass, abundance and fibre size compared between treatments. Average fibre size ranged between 11.9 and 17.7μm in diameter, and 5.0 and 7.8mm in length. Polyester-cotton fabric consistently shed significantly fewer fibres than either polyester or acrylic. However, fibre release varied according to wash treatment with various complex interactions. We estimate over 700,000 fibres could be released from an average 6kg wash load of acrylic fabric. As fibres have been reported in effluent from sewage treatment plants, our data indicates fibres released by washing of clothing could be an important source of microplastics to aquatic habitats. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. BERGMANN USA SOIL SEDIMENT WASHING TECHNOLOGY - APPLICATIONS ANALYSIS REPORT

    EPA Science Inventory

    This document provides an evaluation of the performance of the Bergmann USA Soil/Sediment Washing System and its applicability for the treatment of soils or sediments contaminated with organic and/or inorganic compounds. Both the technical and economic aspects of the technology w...

  5. Association of rumination time with subclinical ketosis in transition dairy cows.

    PubMed

    Kaufman, E I; LeBlanc, S J; McBride, B W; Duffield, T F; DeVries, T J

    2016-07-01

    The objective of this study was to characterize the relationship between rumination and subclinical ketosis (SCK) in transition dairy cows. A study was conducted on 4 commercial dairy farms in eastern Ontario, Canada. A total of 339 Holstein dairy cows (107 primiparous and 232 multiparous) were monitored for rumination activity and SCK from 14 d before calving until 28 d after calving. Rumination was recorded daily using an automated monitoring system. A blood sample was taken from the coccygeal vein of each cow for measurement of β-hydroxybutyrate (BHB) once weekly throughout the 6-wk observation period. Cows with BHB ≥1.2mmol/L in any of the 4 postpartum samples were considered to have SCK. Cases of retained placenta, metritis, milk fever, or mastitis during the study period were also recorded. Cows were categorized into 1 of 4 groups: healthy cows (HLT) that had no SCK or any other recorded health problem (n=139); cows treated for at least one health issue other than SCK (HLT+; n=50); cows with SCK (hyperketonemia; HYK) with no other health problems during transition (n=97); or cows (HYK+) that had SCK and one or more other health problems (n=53). Daily rumination time was summarized by week and comparisons were made between HLT and HYK and HYK+. From 2 wk before calving (wk -2) to 4 wk after calving (wk +4), there was no difference in rumination time (409±9.8min/d) among HLT, HYK, and HYK+ cows in their first lactation. Multiparous cows in HLT spent an average of 459±11.3min/d ruminating from wk -2 to wk +4. Multiparous HYK cows ruminated 25±12.8min/d less than HLT cows, whereas HYK+ cows ruminated 44±15.6min/d less than HLT cows. The largest differences in rumination time between HLT and HYK+ cows were seen during wk -1, +1, and +2, when HYK+ cows ruminated 48±17.2, 73±16.0, and 65±19.4min/d less than HLT cows, respectively. In multiparous cows, increased odds of HYK were associated with greater milk yield in the previous lactation, greater loss of

  6. Cow attributes, herd management, and reproductive history events associated with abortion in cow-calf herds from Western Canada.

    PubMed

    Waldner, C L

    2014-04-01

    The primary objective of this study was to identify herd management and cow characteristics that are associated with abortion in cow-calf herds in Western Canada. Reproductive events were closely monitored in 29,713 cows in 203 herds from the beginning of the breeding season in 2001 through the calving season in 2002. Herd management and cow-level risk factors such as age, body condition score, and previous reproductive history were measured through a series of herd visits by project personnel and detailed individual animal records maintained by the herd owner. Pregnancy status was assessed in fall of 2001 by the herd veterinarian. Cows most likely to abort were replacement heifers, cows that were more than 10 years of age, cows with a body condition score of less than or equal to or 5 of 9 at pregnancy testing, or with twin pregnancies. Cows vaccinated for bovine viral diarrhea virus and infectious bovine rhinotracheitis and bred on community pastures were less likely to abort than cows from community pastures that were not vaccinated. Cows bred on community pastures that were not vaccinated were also more likely to abort than cows that were not on community pastures regardless of vaccination status. Adverse calving-associated events such as severe dystocia, problems such as uterine prolapse or retained placentas, abortion or calf death within 1 hour of birth were also associated with an increased risk of abortion the subsequent calving season after accounting for all other factors. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. Towards bedside washing of stored red blood cells: a prototype of a simple apparatus based on microscale sedimentation in normal gravity.

    PubMed

    Khanal, G; Huynh, R A; Torabian, K; Xia, H; Vörös, E; Shevkoplyas, S S

    2018-01-01

    Infusion of by-products of red blood cell (RBC) storage-induced degradation as well as of the residual plasma proteins and the anticoagulant-preservative solution contained in units of stored blood serve no therapeutic purpose and may be harmful to some patients. Here, we describe a prototype of a gravity-driven system for bedside washing of stored RBCs. Stored RBCs were diluted to 10% haematocrit (Hct) with normal saline, matching the conventional washing procedure. The dilute RBC suspensions were passed through a column of coiled tubing to allow RBC sedimentation in normal gravity, thus separating them from the washing solution. Washed RBCs were collected using bifurcations located along the tubing. Washing efficiency was quantified by measuring Hct, morphology, deformability, free haemoglobin and total-free protein. The gravity-driven washing system operating at 0·5 ml/min produced washed RBCs with final Hct of 36·7 ± 3·4% (32·3-41·2%, n = 10) and waste Hct of 3·4 ± 0·7% (2·4-4·3%, n = 10), while removing 80% of free haemoglobin and 90% of total-free protein. Washing improved the ability of stored RBCs to perfuse an artificial microvascular network by 20%. The efficiency of washing performed using the gravity-driven system was not significantly different than that of conventional centrifugation. This proof-of-concept study demonstrates the feasibility of washing stored RBCs using a simple, disposable system with efficiency comparable to that of conventional centrifugation, and thus represents a significant first step towards enabling low-cost washing of stored blood at bedside. © 2017 International Society of Blood Transfusion.

  8. Effect of different flooring systems on claw conformation of dairy cows.

    PubMed

    Telezhenko, E; Bergsten, C; Magnusson, M; Nilsson, C

    2009-06-01

    The effect of different flooring surfaces in walking and standing areas on claw conformation, claw horn growth, and wear was studied in 2 experiments during 2 consecutive housing seasons in a research dairy herd of 170 cows. In experiment 1, the flooring systems tested were solid rubber mats, mastic asphalt with and without rubber-matted feed-stalls, and aged concrete slats. In experiment 2, slatted concrete flooring was compared with slatted rubber flooring. The cows were introduced to the respective flooring systems in early lactation and their claws were trimmed before the exposure period. Toe length, toe angle, sole concavity, and claw width, as well as claw growth and wear rates were recorded for lateral and medial claws of the left hind limb. Claw asymmetry calculations were based on these claw measurements and on differences in sole protrusion between lateral and medial soles. Asphalt floors caused shorter toe length and steeper toe angle. They also increased wear on rear claws (5.30 +/- 0.31 and 5.95 +/- 0.33 mm/mo for lateral and medial claw, respectively; LSM +/- SE) and horn growth rate (5.12 +/- 0.36 and 5.83 +/- 0.31 mm/mo of lateral and medial claws, respectively). Rubber mats instead of asphalt in walking areas reduced wear (1.36 +/- 0.19 and 2.02 +/- 0.20 mm/mo for lateral and medial claw, respectively) and claw growth (3.83 +/- 0.23 and 3.94 +/- 0.17 mm/mo for lateral and medial claw, respectively). Rubber-matted feed-stalls together with asphalt walkways decreased claw wear (3.29 +/- 0.31 and 4.10 +/- 0.32 mm/mo for lateral and medial claw, respectively). The concavity of claw soles was reduced on asphalt, especially in the lateral rear claws. Rubber matting in feed-stalls prevented loss of sole concavity compared with asphalt. Claw asymmetry did not differ between flooring systems. While different access to abrasive flooring affected claw conformation, there was no evidence that flooring system influenced the disproportion between lateral and

  9. 33 CFR 157.10c - Segregated ballast tanks, crude oil washing systems, and dedicated clean ballast tanks for...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... to meet the draft and trim requirements in § 157.09(b); or (2) A crude oil washing system that meets... trim requirements in § 157.09(b); or (2) Dedicated clean ballast tanks that meet the design and... meet the draft and trim requirements in § 157.09(b). (d) If the arrangement of tanks on a vessel under...

  10. 33 CFR 157.10c - Segregated ballast tanks, crude oil washing systems, and dedicated clean ballast tanks for...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... to meet the draft and trim requirements in § 157.09(b); or (2) A crude oil washing system that meets... trim requirements in § 157.09(b); or (2) Dedicated clean ballast tanks that meet the design and... meet the draft and trim requirements in § 157.09(b). (d) If the arrangement of tanks on a vessel under...

  11. Research notes : bridge washing to reduce salt.

    DOT National Transportation Integrated Search

    2005-10-01

    The Oregon Department of Transportation investigated periodic bridge washing as a way to possibly remove chloride from the concrete and stop further uptake of chloride ions. Washing trials were conducted over a 4-year period on concrete blocks to det...

  12. Propeller wash effects on spray drift

    Treesearch

    Steven J. Thompson; Alvin R. Womac; Joseph Mulrooney; Sidney Deck

    2005-01-01

    for aerial spray application, there is some question if off-target drift (both near and far) is influenced by which boom is spraying and the direction of propeller wash rotation. This information may be useful when switching off one boom close to a field boundary. The effect of alternate boom switching and propeller wash direction on aerial spray drift from a turbine-...

  13. Evaluation of the systemic innate immune response and metabolic alterations of nonlactating cows with diet-induced subacute ruminal acidosis.

    PubMed

    Rodríguez-Lecompte, J C; Kroeker, A D; Ceballos-Márquez, A; Li, S; Plaizier, J C; Gomez, D E

    2014-12-01

    Subacute ruminal acidosis (SARA) increases lipopolysaccharide endotoxin in the rumen, which might translocate into the systemic circulation, triggering a cascade of clinical and immunological alterations. The objective of this study was to characterize the clinical immune and metabolic responses to ruminal-derived lipopolysaccharide in nonlactating cows induced with SARA using 2 challenges, a grain-based SARA challenge (GBSC) or an alfalfa-pellet SARA challenge (APSC). Six dry, nonlactating Holstein cows were used in a 3 × 3 Latin square arrangement of treatments with 4-wk experimental cycles. All cows received the control diet containing 70% forage and 30% mixed concentrates (dry matter basis) for 3 wk. In wk 4, cows received a control diet, GBSC (38% wheat-barley pellets, 32% other mixed concentrate, and 30% forages), or APSC (45% mixed concentrate, 32% alfalfa pellets, and 23% other forages). Total plasma proteins and immunology-related proteins, acute phase proteins, blood cells, serum chemistry, mRNA gene expression of peripheral blood cell surface markers, and selected proinflammatory cytokines were evaluated. Ruminal pH was lower in both groups with induced SARA compared with a control group. Ruminal endotoxins were higher in GBSC; however, plasma endotoxin was not detected in any study group. No significant differences in feed intake, rectal temperature, white blood cell counts, or differentials were found between control and SARA challenge groups; changes in glucose, urea, Ca, and Mg were observed in SARA groups. Total plasma proteins were lower in both SARA groups, and acute phase proteins were higher in GBSC. The expression of CD14, MD2, and TLR4 mRNA in peripheral blood leukocytes was not affected by SARA induction. The induction of SARA as a result of GBSC or APSC challenge was successful; however, LPS was not detected in plasma. Changes in clinical, metabolic, and inflammatory responses were not observed in the SARA-challenged cows, suggesting that

  14. Technical note: development and testing of a radio transmission pH measurement system for continuous monitoring of ruminal pH in cows.

    PubMed

    Sato, Shigeru; Mizuguchi, Hitoshi; Ito, Kazunori; Ikuta, Kentaro; Kimura, Atushi; Okada, Keiji

    2012-03-01

    An indwelling ruminal pH system has been used for the continuous recording of ruminal pH to evaluate subacute ruminal acidosis (SARA) in dairy cows. However this system does not allow the field application. The objective of this study was to develop a new radio transmission pH measurement system, and to assess its performance and usefulness in a continuous evaluation of ruminal pH for use on commercial dairy farms. The radio transmission pH measurement system consists of a wireless pH sensor, a data measurement receiver, a relay unit, and a personal computer installed special software. The pH sensor is housed in a bullet shaped bolus, which also encloses a pH amplifier circuit, a central processing unit (CPU) circuit, a radio frequency (RF) circuit, and a battery. The mean variations of the measurements by the glass pH electrode were +0.20 (n=10) after 2 months of continuous recording, compared to the values confirmed by standard pH solutions for pH 4 and pH 7 at the start of the recording. The mean lifetime of the internal battery was 2.5 months (n=10) when measurements were continuously transmitted every 10 min. Ruminal pH recorded by our new system was compared to that of the spot sampling of ruminal fluid. The mean pH for spot sampling was 6.36 ± 0.55 (n=96), and the mean pH of continuous recording was 6.22 ± 0.54 (n=96). There was a good correlation between continuous recording and spot sampling (r=0.986, P<0.01). We also examined whether our new pH system was able to detect experimentally induced ruminal acidosis in cows and to record long-term changes in ruminal pH. In the cows fed acidosis-inducing diets, the ruminal pH dropped markedly during the first 2h following the morning feeding, and decreased moreover following the evening feeding, with many pulse-like pH changes. The pH of the cows showed the lowest values of 5.3-5.2 in the midnight time period and it recovered to the normal value by the next morning feeding. In one healthy periparturient cow

  15. Impact of Distillery Spent Wash Irrigation on Agricultural Soil

    NASA Astrophysics Data System (ADS)

    Jadhav, Ramanand N.; Sarode, Dhananjay B.; Narkhede, Sachin D.; Khatik, Vasimshaikh A.; Attarde, Sanjay B.

    2011-07-01

    The disposal of wastes from industrial sources is becoming a serious problem throughout the world. In India, a total of approximately 40 million m3 of distillery spent wash is generated annually from 295 distilleries. The distillery spent wash is acidic and high levels of biological oxygen demand and chemical oxygen demand and contains nutrient elements such as potassium (K), nitrogen (N), and phosphorous (P). It is used as a source of plant nutrients and organic matter for various agricultural crops. It is usually applied to arable land near the distilleries as irrigation water or as a soil amendment. However, indiscriminate disposal of it has resulted in adverse impact on soil environments. This paper aims to identify the impact of distillery spent wash application for irrigation and on soil environment. The distillery spent wash can be a good source of nutrients necessary for plant growth. Application of various concentrations of spent wash on plant species was studied. A plot having 20-30% concentration of spent wash observed good growth. At higher doses, spent wash application is found harmful to crop growth and soil fertility and its use at lower doses remarkably improves germination and growth of crops.

  16. Isolating the cow-specific part of residual energy intake in lactating dairy cows using random regressions.

    PubMed

    Fischer, A; Friggens, N C; Berry, D P; Faverdin, P

    2018-07-01

    The ability to properly assess and accurately phenotype true differences in feed efficiency among dairy cows is key to the development of breeding programs for improving feed efficiency. The variability among individuals in feed efficiency is commonly characterised by the residual intake approach. Residual feed intake is represented by the residuals of a linear regression of intake on the corresponding quantities of the biological functions that consume (or release) energy. However, the residuals include both, model fitting and measurement errors as well as any variability in cow efficiency. The objective of this study was to isolate the individual animal variability in feed efficiency from the residual component. Two separate models were fitted, in one the standard residual energy intake (REI) was calculated as the residual of a multiple linear regression of lactation average net energy intake (NEI) on lactation average milk energy output, average metabolic BW, as well as lactation loss and gain of body condition score. In the other, a linear mixed model was used to simultaneously fit fixed linear regressions and random cow levels on the biological traits and intercept using fortnight repeated measures for the variables. This method split the predicted NEI in two parts: one quantifying the population mean intercept and coefficients, and one quantifying cow-specific deviations in the intercept and coefficients. The cow-specific part of predicted NEI was assumed to isolate true differences in feed efficiency among cows. NEI and associated energy expenditure phenotypes were available for the first 17 fortnights of lactation from 119 Holstein cows; all fed a constant energy-rich diet. Mixed models fitting cow-specific intercept and coefficients to different combinations of the aforementioned energy expenditure traits, calculated on a fortnightly basis, were compared. The variance of REI estimated with the lactation average model represented only 8% of the variance of

  17. The relationship between dairy cow hygiene and somatic cell count in milk.

    PubMed

    Sant'anna, A C; Paranhos da Costa, M J R

    2011-08-01

    Corporal hygiene is an important indicator of welfare for dairy cows and is dependent on facilities, climate conditions, and the behavior of the animals. The objectives of this study were to describe how the hygiene conditions of dairy cows vary over time and to assess whether a relationship exists between hygiene and somatic cell count (SCC) in milk. Monthly hygiene evaluations were conducted on lactating cows in 2 dairy farms for 9 consecutive months, totaling 3,554 evaluations from 545 animals. Hygiene was measured using a 4-point scoring system (very clean, clean, dirty, and very dirty) for 4 areas of the animal's body (leg, flank, abdomen, and udder) and combining these scores to generate a composite cleanliness score. A total of 2,218 milk samples was analyzed from 404 cows to determine SCC and somatic cell linear scores (SCLS). Individual variation was observed in the hygiene of cows throughout the year, with the highest proportion of clean cows being observed in August and the lowest in January. In spite of this seasonal variation, approximately half (55.62%) of the cows displayed consistent cleanliness scores, with 45.86% of them remaining consistently clean (very clean or clean) and 9.76% remaining dirty (very dirty or dirty) over the course of the study. The very clean cows had the lowest SCLS, followed by the clean, dirty, and very dirty cows (no statistically significant differences were found between the latter 2 groups). The most critical months for cow hygiene were those with the greatest rainfall, when a reduction in the welfare of cows and higher SCC values were observed. The evaluation and control of dairy cow hygiene are useful in defining management strategies to reduce problems with milk and improve the welfare of the animals. Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  18. Cow's milk challenge through human milk evokes immune responses in infants with cow's milk allergy.

    PubMed

    Järvinen, K M; Mäkinen-Kiljunen, S; Suomalainen, H

    1999-10-01

    In order to measure the immune response evoked in breast-fed infants with cow's milk allergy (CMA) by cow's milk challenge through human milk, mothers were given increasing doses of cow's milk after they had been on a cow's milk elimination diet. Another objective was to study the secretion of beta-lactoglobulin (BLG) into human milk before and during milk challenge in relation to the appearance of symptoms in infants. Seventeen asymptomatic mothers who had infants with challenge-proven CMA and 10 asymptomatic mothers who had healthy infants were recruited. Infants ranged in age from 1.8 to 9.4 months. A solid-phase enzyme-linked immunoassay (ELISPOT) was used to assess the total number of immunoglobulin-secreting and specific antibody-secreting cells. Flow cytometry was used to enumerate different lymphocyte subpopulations among peripheral blood lymphocytes primed during provocation by cow's milk antigens. BLG levels were assessed in human milk before the challenge and 1, 2, 3, and 4 hours after the commencement of the challenge. All but one of the infants with CMA showed symptoms of CMA during cow's milk challenge through human milk. There was a significant rise in the total number of immunoglobulin-secreting cells in the IgA and IgG classes associated with a positive cow's milk challenge response, but the proportions of peripheral blood B cells bearing CD19, CD23, CD19 and 23, CD5, or CD19 and CD5 were comparable. BLG levels were comparable in both study groups. Most of the infants with CMA reacted to cow's milk challenge through human milk. Hypersensitivity reactions to food antigens through human milk may be more common than previously thought.

  19. Faecal bacterial composition in dairy cows shedding Mycobacterium avium subsp. paratuberculosis in faeces in comparison with nonshedding cows.

    PubMed

    Kaevska, Marija; Videnska, Petra; Sedlar, Karel; Bartejsova, Iva; Kralova, Alena; Slana, Iva

    2016-06-01

    The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by real-time PCR, and faecal bacterial populations were analysed by 454 pyrosequencing of the 16S rRNA gene. The most notable differences between shedding and nonshedding cows were an increase in the genus Psychrobacter and a decrease in the genera Oscillospira, Ruminococcus, and Bifidobacterium in cows infected with M. avium subsp. paratuberculosis. The present study is the first to report the faecal microbial composition in dairy cows infected with M. avium subsp. paratuberculosis.

  20. 21 CFR 133.137 - Washed curd cheese for manufacturing.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Washed curd cheese for manufacturing. 133.137... (CONTINUED) FOOD FOR HUMAN CONSUMPTION CHEESES AND RELATED CHEESE PRODUCTS Requirements for Specific Standardized Cheese and Related Products § 133.137 Washed curd cheese for manufacturing. Washed curd cheese for...

  1. 21 CFR 133.137 - Washed curd cheese for manufacturing.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Washed curd cheese for manufacturing. 133.137... (CONTINUED) FOOD FOR HUMAN CONSUMPTION CHEESES AND RELATED CHEESE PRODUCTS Requirements for Specific Standardized Cheese and Related Products § 133.137 Washed curd cheese for manufacturing. Washed curd cheese for...

  2. Identification of cow milk in goat milk by nonlinear chemical fingerprint technique.

    PubMed

    Ma, Yong-Jie; Dong, Wen-Bin; Fan, Cheng; Wang, Er-Dan

    2017-10-01

    The objective of this paper was to develop a nonlinear chemical fingerprint technique for identifying and detecting adulteration of goat milk with cow milk. In this study, by taking the Belousov-Zhabotinsky oscillatory chemical reaction using acetone and substrates in goat milk or cow milk as main dissipative substances, when the same dosage of goat milk and cow milk was introduced to the "H +  + Mn 2+  + BrO 3 -  + acetone" oscillating system respectively, nonlinear chemical fingerprints were obtained for goat milk and cow milk from the same origin. The results showed that inductive time value and the content of cow milk in goat milk had a linear relationship in the range of 0-100% and the corresponding regression coefficient was 0.9991. A detection limit of 0.0107 g/g was obtained, and the content of cow milk in mixed milk was calculated. The proposed method in this study was simple, economical and effective. In addition, the method did not need the pretreatment and separation of samples for identifying and evaluating cow milk adulteration in goat milk. Copyright © 2017. Published by Elsevier B.V.

  3. Experimentally induced mastitis and metritis modulate soy bean derived isoflavone biotransformation in diary cows.

    PubMed

    Kowalczyk-Zieba, I; Woclawek-Potocka, I; Piskula, M K; Piotrowska-Tomala, K K; Boruszewska, D; Bah, M M; Siemieniuch, M J; Skarzynski, D J

    2011-12-01

    The present study compared the changes in isoflavone (daidzein and genistein) and their metabolite (equol and para-ethyl-phenol) concentrations in the blood plasma of cows with induced mastitis and metritis after feeding with soy bean. Sixteen cows were divided into four groups: control for mastitis group, cows with induced mastitis group, control for metritis group, and cows with induced metritis group. All cows were fed a single dose of 2.5 kg of soy bean and then blood samples were taken from the jugular vein for 8 h at predetermined intervals. The concentrations of soy bean-derived isoflavones and their active metabolites were measured in the blood plasma on HPLC system. β-Glucuronidase activity in the blood plasma of cows was measured by fluorometric method. In the blood plasma of cows with induced mastitis and metritis, we found considerably higher concentrations and time-dependent increase in isoflavone metabolites (equol and para-ethyl-phenol) with reference to cyclic cows (P < 0.05). Moreover, we noticed significant decrease of genistein in the blood plasma of the cows with induced metritis compared with control cows (P < 0.05). In addition, in the blood plasma of the cows with induced metritis, we found an increase in β-glucuronidase activity compared with control cows (P < 0.05). In conclusion, health status of the females influenced the concentrations of isoflavone metabolites in the blood plasma of the cows. Experimentally induced mastitis and metritis increased isoflavone absorption, biotransformation and metabolism. Therefore, we suggest that cows with induced mastitis and metritis are more exposed to active isoflavone metabolite actions than healthy cows. Copyright © 2011. Published by Elsevier Inc.

  4. Cockrell washs hair and face

    NASA Image and Video Library

    1996-12-16

    STS080-312-004 (19 Nov.-7 Dec. 1996) --- Astronaut Kenneth D. Cockrell, STS-80 mission commander, washes his hair on the middeck of the Earth-orbiting space shuttle Columbia. Displaying a sense of humor, the commander asked astronaut Story Musgrave, who is bald, to address this visual during a briefing with Johnson Space Center (JSC) employees on Jan. 14, 1997. Equal to the task, Musgrave cracked a number of bald jokes and remarked that it was much easier to polish a head in zero gravity than to wash one.

  5. Investigation of bovine venereal campyloacteriosis in beef cow herds in New Zealand.

    PubMed

    McFadden, A M; Heuer, C; Jackson, R; West, D M; Parkinson, T J

    2005-02-01

    To determine regional prevalences of beef cow herds in New Zealand positive for Campylobacter fetus subsp venerealis antibodies in samples of vaginal mucus tested using an immunoglobulin (Ig) A enzyme-linked immunosorbent assay (ELISA), and to examine the suitability of the IgA ELISA for detecting infection with C. fetus subsp venerealis under field conditions in New Zealand. Vaginal mucus samples (n=1,230) collected from beef cow herds (n=125) throughout New Zealand (approximately 10 samples/herd) were tested for antibodies to C. fetus subsp venerealis using an IgA ELISA. Test results were compared between herds classified as having low, medium and high fertility based on pregnancy test results interpreted in relation to the duration of the mating period used. In addition, a small number of samples were collected from dairy cows that were mated using artificial insemination (AI) and had no contact with breeding bulls. The influence of putative risk factors for the spread of venereal disease and the effect of sample quality on the status of herds according to test results was assessed using multivariate logistical regression. Preputial washings from 54 bulls from nine herds classified as low fertility in which mucus samples from > or =3 cows were IgA antibody-positive were cultured for the presence of Campylobacter spp, and isolates of C. fetus subspecies were characterised using a polymerase chain reaction (PCR) test. One or more mucus samples was positive to the IgA ELISA in 70% of all herds tested. The prevalence of IgA antibody- positive individuals was >20% in most regions of New Zealand and did not differ significantly for cows from herds classified as high, medium or low fertility (28%, 26% and 23%, respectively; p=0.39). No relationship was found between mucus antibody status and age of breeding group, herd size, herd fertility, number of herds that female replacements or breeding bulls were sourced from, whether a serving ability test (SAT) was used to

  6. Whole cow's milk in early life.

    PubMed

    Thorsdottir, Inga; Thorisdottir, Asa V

    2011-01-01

    Cow's milk is a major food for young children. Whole cow's milk is known to be detrimental to infants, mainly due to its low iron content. The negative association with iron status led to recommending the introduction of formula feeding in infancy during the weaning period or when breastfeeding ceased. More recently, the literature suggests that consuming whole cow's milk in infancy has unfortunate effects on growth, especially weight acceleration and development of overweight in childhood. These issues are discussed in the following chapter. Other suggested reasons for the avoidance of whole cow's milk in infancy are touched upon, such as milk protein allergy and high renal solute load. The hypothesis about early cow's milk introduction in the pathology of certain diseases, mainly through the peptide β-casomorphin-7, is briefly reviewed, showing that there is no clear evidence for the suggested associations. The chapter gives a recent example of introducing formula at 6 months of age instead of whole cow's milk in infants' diet in Iceland. Several aspects of consuming whole cow's milk in infancy can be found in recent reviews. Copyright © 2011 S. Karger AG, Basel.

  7. A field study to determine the prevalence, dairy herd management systems, and fresh cow clinical conditions associated with ketosis in western European dairy herds.

    PubMed

    Berge, Anna C; Vertenten, Geert

    2014-01-01

    The aim of this study was to determine the prevalence, major management systems, and fresh cow clinical conditions associated with ketosis in western European dairy herds. A total of 131 dairies were enrolled in Germany, France, Italy, the Netherlands, and the United Kingdom during 2011 to 2012. A milk-based test for ketones (Keto-Test; Sanwa Kagaku Kenkyusho Co. Ltd., Nagoya, Japan; distributed by Elanco Animal Health, Antwerp, Belgium) was used for screening cows between d 7 and 21 after calving and ketosis was defined as a Keto-Test ≥100µmol/L. Study cows were observed for clinical disease up to 35d postcalving. Multivariate analysis (generalized estimating equation logistic regression) was performed to determine country, farm, management, feed, and cow factors associated with ketosis and to determine associations between ketosis and fresh cow diseases. Thirty-nine percent of the cows were classified as having ketosis. The herd average of ketosis was 43% in Germany, 53% in France, 31% in Italy, 46% in the Netherlands, and 31% in the United Kingdom. Of the 131 farms, 112 (85%) had 25% or more of their fresh cows resulting as positive for ketosis. Clinical ketosis was not reported in most farms and the highest level of clinical ketosis reported was 23%. The risks of ketosis were significantly lower in Italy and the United Kingdom compared with France, the Netherlands, and Germany. Larger herd size was associated with a decreased risk of ketosis. The farms that fed partially mixed rations had 1.5 times higher odds of ketosis than those that fed total mixed rations. Cows that calved in April to June had the highest odds of ketosis, with about twice as high odds compared with cows that calved in July to September. The cows that calved in January to March tended to have 1.5 times higher risk of ketosis compared with cows that calved in July to September. The odds of ketosis in parity 2 and parity 3 to 7 was significantly higher (1.5 and 2.8 times higher

  8. [Automated parturition control in primi- and multiparous cows of a Simmental and Holstein crossbred herd].

    PubMed

    Dippon, Matthias; Petzl, Wolfram; Lange, Dorothee; Zerbe, Holm

    2017-02-09

    Perinatal calf mortality is a current problem in dairy farming with regards to ethics and economic losses. Optimizing calving management by frequent monitoring helps increasing the survival rate. The objective of this study was to evaluate the breed and parity dependent applicability of a recently introduced automated parturition control system with regards to its reliability in the field. Seven days prior to the calculated calving date the automated parturition control system was applied intravaginally in 23 primiparous and 31 multiparous cows in a Holstein-Friesian (HF) and Simmental (FV) crossbred herd. In the case of three consecutive false alarms the animal was removed from the study and was rated as false positive (FP). The statistical significant interdependence of FP alarms and the genetic proportion of HF was calculated using the Mann-Whitney-U test. The automated parturition control system could successfully be applied in all animals with a genetic HF proportion > 66%. Animals with a predominant FV proportion (> 66%) frequently showed FP alarms (31.6%). Furthermore, multiparous cows lost the intravaginal sender more frequently than primiparous cows (29.0% vs. 8.7%). In 72.2% heavily pregnant cows purulent vaginal discharge was observed. The automated parturition control system can successfully be applied in HF cows. Due to frequent losses of the intravaginal sender we cannot recommend its use in cows with a genetic FV proportion > 66%. Future developments of intravaginal automated parturition control systems should incorporate the influence of different breeds on its applicability.

  9. Changes in soil toxicity by phosphate-aided soil washing: effect of soil characteristics, chemical forms of arsenic, and cations in washing solutions.

    PubMed

    Jho, Eun Hea; Im, Jinwoo; Yang, Kyung; Kim, Young-Jin; Nam, Kyoungphile

    2015-01-01

    This study was set to investigate the changes in the toxicity of arsenic (As)-contaminated soils after washing with phosphate solutions. The soil samples collected from two locations (A: rice paddy and B: forest land) of a former smelter site were contaminated with a similar level of As. Soil washing (0.5 M phosphate solution for 2 h) removed 24.5% As, on average, in soil from both locations. Regardless of soil washing, Location A soil toxicities, determined using Microtox, were greater than that of Location B and this could be largely attributed to different soil particle size distribution. With soils from both locations, the changes in As chemical forms resulted in either similar or greater toxicities after washing. This emphasizes the importance of considering ecotoxicological aspects, which are likely to differ depending on soil particle size distribution and changes in As chemical forms, in addition to the total concentration based remedial goals, in producing ecotoxicologically-sound soils for reuse. In addition, calcium phosphate used as the washing solution seemed to contribute more on the toxic effects of the washed soils than potassium phosphate and ammonium phosphate. Therefore, it would be more appropriate to use potassium or ammonium phosphate than calcium phosphate for phosphate-aided soil washing of the As-contaminated soils. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Comparison of pharmacokinetics and milk elimination of flunixin in healthy cows and cows with mastitis.

    PubMed

    Kissell, Lindsey W; Leavens, Teresa L; Baynes, Ronald E; Riviere, Jim E; Smith, Geof W

    2015-01-01

    To determine whether pharmacokinetics and milk elimination of flunixin and 5-hydroxy flunixin differed between healthy and mastitic cows. Prospective controlled clinical trial. 20 lactating Holstein cows. Cows with mastitis and matched control cows received flunixin IV, ceftiofur IM, and cephapirin or ceftiofur, intramammary. Blood samples were collected before (time 0) and 0.25, 0.5, 1, 2, 4, 8, 12, 24, and 36 hours after flunixin administration. Composite milk samples were collected at 0, 2, 12, 24, 36, 48, 60, 72, 84, and 96 hours. Plasma and milk samples were analyzed by use of ultra-high-performance liquid chromatography with mass spectrometric detection. For flunixin in plasma samples, differences in area under the concentration-time curve and clearance were detected between groups. Differences in flunixin and 5-hydroxy flunixin concentrations in milk were detected at various time points. At 36 hours after flunixin administration (milk withdrawal time), 8 cows with mastitis had 5-hydroxy flunixin concentrations higher than the tolerance limit (ie, residues). Flunixin residues persisted in milk up to 60 hours after administration in 3 of 10 mastitic cows. Pharmacokinetics and elimination of flunixin and 5-hydroxy flunixin in milk differed between mastitic and healthy cows, resulting in violative residues. This may partially explain the high number of flunixin residues reported in beef and dairy cattle. This study also raised questions as to whether healthy animals should be used when determining withdrawal times for meat and milk.

  11. 'If an Eye Is Washed Properly, It Means It Would See Clearly': A Mixed Methods Study of Face Washing Knowledge, Attitudes, and Behaviors in Rural Ethiopia.

    PubMed

    Aiemjoy, Kristen; Stoller, Nicole E; Gebresillasie, Sintayehu; Shiferaw, Ayalew; Tadesse, Zerihun; Sewnet, Tegene; Ayele, Bezuayehu; Chanyalew, Melsew; Callahan, Kelly; Stewart, Aisha; Emerson, Paul M; Lietman, Thomas M; Keenan, Jeremy D; Oldenburg, Catherine E

    2016-10-01

    Face cleanliness is a core component of the SAFE (Surgery, Antibiotics, Facial cleanliness, and Environmental improvements) strategy for trachoma control. Understanding knowledge, attitudes, and behaviors related to face washing may be helpful for designing effective interventions for improving facial cleanliness. In April 2014, a mixed methods study including focus groups and a quantitative cross-sectional study was conducted in the East Gojjam zone of the Amhara region of Ethiopia. Participants were asked about face washing practices, motivations for face washing, use of soap (which may reduce bacterial load), and fly control strategies. Overall, both knowledge and reported practice of face washing was high. Participants reported they knew that washing their own face and their children's faces daily was important for hygiene and infection control. Although participants reported high knowledge of the importance of soap for face washing, quantitative data revealed strong variations by community in the use of soap for face washing, ranging from 4.4% to 82.2% of households reporting using soap for face washing. Cost and forgetfulness were cited as barriers to the use of soap for face washing. Keeping flies from landing on children was a commonly cited motivator for regular face washing, as was trachoma prevention. Interventions aiming to improve facial cleanliness for trachoma prevention should focus on habit formation (to address forgetfulness) and address barriers to the use of soap, such as reducing cost. Interventions that focus solely on improving knowledge may not be effective for changing face-washing behaviors.

  12. 21 CFR 133.137 - Washed curd cheese for manufacturing.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Washed curd cheese for manufacturing. 133.137 Section 133.137 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... Standardized Cheese and Related Products § 133.137 Washed curd cheese for manufacturing. Washed curd cheese for...

  13. Japanese encephalitis in a 114-month-old cow: pathological investigation of the affected cow and genetic characterization of Japanese encephalitis virus isolate

    PubMed Central

    2014-01-01

    Background Japanese encephalitis virus (JEV) is classified into the genus Flavivirus in the family Flaviviridae. JEV can cause febrile illness and encephalitis mainly in humans and horses, and occasionally in cattle. Case presentation In late September 2010, a 114-month-old cow showed neurological symptoms similar to the symptoms observed in previous bovine cases of Japanese encephalitis (JE); therefore, we conducted virological and pathological tests on the cow. As a result, JEV was isolated from the cerebrum of the affected cow. We determined the complete genome sequence of the JEV isolate, which we named JEV/Bo/Aichi/1/2010, including the envelope (E) gene region and 3’ untranslated region (3’UTR). Our phylogenetic analyses of the E region and complete genome showed that the isolate belongs to JEV genotype 1 (G1). The isolate, JEV/Bo/Aichi/1/2010, was most closely related to several JEV G1 isolates in Toyama Prefecture, Japan in 2007–2009 by the phylogenetic analysis of the E region. In addition, the nucleotide alignment revealed that the deletion in the 3’UTR was the same between JEV/Bo/Aichi/1/2010 and several other JEV G1 isolates identified in Toyama Prefecture in 2008–2009. A hemagglutination inhibition (HI) test was conducted for the detection of anti-JEV antibodies in the affected cow, and the test detected 2-mercaptoethanol (2-ME)-sensitive HI antibodies against JEV in the serum of the affected cow. The histopathological investigation revealed nonsuppurative encephalomyelitis in the affected cow, and the immunohistochemical assay detected JEV antigen in the cerebrum. Conclusion We diagnosed the case as JE of a cow based on the findings of nonsuppurative encephalomyelitis observed in the central nervous system, JEV antigen detected in the cerebrum, JEV isolated from the cerebrum, and 2-ME-sensitive HI antibodies against JEV detected in the serum. This is the first reported case of JE in a cow over 24 months old. PMID:24618225

  14. Japanese encephalitis in a 114-month-old cow: pathological investigation of the affected cow and genetic characterization of Japanese encephalitis virus isolate.

    PubMed

    Kako, Naomi; Suzuki, Seiji; Sugie, Norie; Kato, Tomoko; Yanase, Tohru; Yamakawa, Makoto; Shirafuji, Hiroaki

    2014-03-11

    Japanese encephalitis virus (JEV) is classified into the genus Flavivirus in the family Flaviviridae. JEV can cause febrile illness and encephalitis mainly in humans and horses, and occasionally in cattle. In late September 2010, a 114-month-old cow showed neurological symptoms similar to the symptoms observed in previous bovine cases of Japanese encephalitis (JE); therefore, we conducted virological and pathological tests on the cow. As a result, JEV was isolated from the cerebrum of the affected cow. We determined the complete genome sequence of the JEV isolate, which we named JEV/Bo/Aichi/1/2010, including the envelope (E) gene region and 3' untranslated region (3'UTR). Our phylogenetic analyses of the E region and complete genome showed that the isolate belongs to JEV genotype 1 (G1). The isolate, JEV/Bo/Aichi/1/2010, was most closely related to several JEV G1 isolates in Toyama Prefecture, Japan in 2007-2009 by the phylogenetic analysis of the E region. In addition, the nucleotide alignment revealed that the deletion in the 3'UTR was the same between JEV/Bo/Aichi/1/2010 and several other JEV G1 isolates identified in Toyama Prefecture in 2008-2009. A hemagglutination inhibition (HI) test was conducted for the detection of anti-JEV antibodies in the affected cow, and the test detected 2-mercaptoethanol (2-ME)-sensitive HI antibodies against JEV in the serum of the affected cow. The histopathological investigation revealed nonsuppurative encephalomyelitis in the affected cow, and the immunohistochemical assay detected JEV antigen in the cerebrum. We diagnosed the case as JE of a cow based on the findings of nonsuppurative encephalomyelitis observed in the central nervous system, JEV antigen detected in the cerebrum, JEV isolated from the cerebrum, and 2-ME-sensitive HI antibodies against JEV detected in the serum. This is the first reported case of JE in a cow over 24 months old.

  15. Pollutant removal from oily wastewater discharged from car washes through sedimentation-coagulation.

    PubMed

    Rubí, H; Fall, C; Ortega, R E

    2009-01-01

    Wastewater from car washes represents a potential problem for the sewer system due to its emulsified oils and suspended material. Treatment of wastewater discharged from four car washes was investigated by sedimentation and coagulation. The effect of the coagulants Servical P (aluminium hydroxychloride), Servican 50 (poly(diallyldimethylammonium chloride)), aluminium sulfate and ferric chloride was evaluated. The achieved removal using sedimentation was of 82%, 88% 73% and 51% for oils, total suspended solids, COD, and turbidity, respectively. In the treatment by coagulation we achieved average efficiencies nearly to 74% for COD removal, greater than 88% in the case of total suspended solids removal and 92% in the case of turbidity and except the performance of Servican 50 greater than 90% in oil removal. We concluded that the oil residual concentration and COD in the treated water allows pouring it in the sewer system complying with the limits of the Mexican rule NOM-002-ECOL-1996 and it is possible even its reuse, at least in the case of the chassis washing of cars.

  16. Spatial and temporal free-ranging cow behaviour pre and post-weaning

    USDA-ARS?s Scientific Manuscript database

    Global positioning system (GPS) technology can be used to study free-ranging cow behaviors. GPS equipment was deployed on each of ten cows ranging in age from 3 to 15 years in order to compare and contrast mean ± standard errors for pre- and post-weaning travel (m·time-1) in two similar (= 433 ha) a...

  17. Treatment of car wash wastewater by UF membranes

    NASA Astrophysics Data System (ADS)

    Istirokhatun, Titik; Destianti, Puti; Hargianintya, Adenira; Oktiawan, Wiharyanto; Susanto, Heru

    2015-12-01

    The existence of car wash service facilitates car owners to remove dirt and grime from their vehicles. However, the dirt washed off vehicles as well as the cleaning materials themselves may be harmful to the environment if they are not properly managed and discharged. Many technologies have been proposed to treat car wash wastewater such as coagulation flocculation, tricking filter and flocculation-flotation. Nevertheless, these technologies have low efficiency to eliminate oil and small organic compounds. Ultrafiltration (UF) membranes were used in this study to treat car wash wastewater. This study investigated the performance of UF membranes under various pressures to remove COD, oil and grease, and also turbidity from car wash waste water. The membrane performance was examined by investigation of permeate flux and membrane rejection. The results meet the standard of environmental regulation and it is possible to be reused. The highest rejection was shown by PES10 (polyethersulfone 10 kDa) in 1 bar operation with complete rejection for both turbidity and oil and grace and 95% rejection for COD.

  18. Prepartum and postpartum nutritional management to optimize fertility in high-yielding dairy cows in confined TMR systems.

    PubMed

    Drackley, J K; Cardoso, F C

    2014-05-01

    The 6 to 8-week period centered on parturition, known as the transition or periparturient period, is critical to welfare and profitability of individual cows. Fertility of high-producing cows is compromised by difficult transitions. Deficiencies in either nutritional or non-nutritional management increase risk for periparturient metabolic disorders and infectious diseases, which decrease subsequent fertility. A primary factor impeding fertility is the extent of negative energy balance (NEB) early postpartum, which may inhibit timing of first ovulation, return to cyclicity, and oocyte quality. In particular, pronounced NEB during the first 10 days to 2 weeks (the time of greatest occurrence of health problems) is critical for later reproductive efficiency. Avoiding over-conditioning and preventing cows from over-consuming energy relative to their requirements in late gestation result in higher dry matter intake (DMI) and less NEB after calving. A pooled statistical analysis of previous studies in our group showed that days to pregnancy are decreased (by 10 days) by controlling energy intake to near requirements of cows before calving compared with allowing cows to over-consume energy. To control energy intake, total mixed rations (TMR) must be well balanced for metabolizable protein, minerals and vitamins yet limit total DM consumed, and cows must uniformly consume the TMR without sorting. Dietary management to maintain blood calcium and rumen health around and after calving also are important. Opportunities may exist to further improve energy status in fresh cows. Recent research to manipulate the glucogenic to lipogenic balance and the essential fatty acid content of tissues are intriguing. High-producing cows that adapt successfully to lactation can have high reproductive efficiency, and nutritional management of the transition period both pre- and post-calving must facilitate that adaptation.

  19. Prepartum Lying Behavior of Holstein Dairy Cows Housed on Pasture through Parturition

    PubMed Central

    Rice, Christa A.; Eberhart, Nicole L.; Krawczel, Peter D.

    2017-01-01

    Simple Summary Dairy cows experience meaningful biological changes during gestation that impact cow comfort and alter behavior, particularly during late gestation and leading up to calving. The housing environment can also have a positive or negative effect on cow comfort. Pasture access allows cows the freedom of movement and an ability to express natural grazing and resting behaviors. After observing cows housed on pasture during the late gestation and calving periods, this study found that lying behaviors only differed on the day of calving and the day prior to calving. Additionally, the proportion of time spent lying per hour decreased in the hour prior to calving compared to 6 h prior to parturition. The altered lying behaviors and activity observed in the hours before calving may indicate a decrease in cow comfort experienced by the cow during parturition. However, discomfort is typical of parturition. These data suggest that cows were able to express natural behaviors associated with calving and pasture when provided an adequate environment for cows during the prepartum period. Abstract Utilizing pasture-based systems may increase cow comfort during late gestation and calving as it lacks the constraints of confinement housing. The objective of this study was to quantify lying behavior and activity of Holstein dairy cows housed on pasture during the 6 d before calving. Sixteen Holstein dairy cows were moved to pasture 3 weeks before their projected calving date. Data loggers were attached 14 d prior to projected calving date. Behavior was evaluated 6 d before calving for all cows (n = 16) and 6 h prior to calving for a subset of cows (n = 6) with known calving times. Data loggers recorded at 1-min intervals to determine lying time (h/d and %/h), lying bouts (n/d and n/h), lying bout duration (min/bout), and steps (n/d and n/h). A repeated measures analysis of variance with contrasts was performed to determine if lying behaviors and activity differed between

  20. Effect of strategic feed supplementation on productive and reproductive performance in yak cows.

    PubMed

    Long, R J; Zhang, D G; Wang, X; Hu, Z Z; Dong, S K

    1999-01-27

    A total of 2230 yak cows (5-13 years of age) in two populations with different milking systems were investigated. One population had a system of milking once a day (MOD), and the other population twice a day (MTD). The average milk yield of MOD cows was 0.7 +/- 0.2 kg/day within a milking period of 109 +/- 9 days. This compared with an average of 1.24 +/- 0.3 kg/day in 127 +/- 6 days in MTD yaks (p < 0.01). The cows showed a calving rate of 71% under the MOD system and 51.4% under the MTD system. Three farms with a total of 104 MTD cows between six and 12 years of age were used to provide three different feeding groups. The groups were fed with, or without oat hay or highland barley straw in amounts of 1-1.5 kg/head/day from December to April. The three farms were designated as Farm I, Farm II, and Farm III. Farm I had 41 cows with body weight of 230 +/- 67 kg each for grazing with no supplement (GNS). Farm II had 30 animals with body weight of 216 +/- 28 kg each for grazing + oat hay (GOH). Farm III had 33 animals body weight of 221 +/- 34 kg each for grazing + highland barely straw (GBS). The calving rates of the cows in GOH and GBS were 23 and 19% higher, respectively, than GNS cows (p < 0.01), and the highest rate reached 76.7% in GOH. The live weight loss of the cows in GNS was considerably higher (p < 0.01) than in the two other groups. Ten GOH cows and 12 GNS cows were used to collect milk samples for measuring the progesterone concentration using RIA kits provided by IAEA/FAO: Milk was sampled every five days from calving until 90 days postpartum. In the unsupplemented group, milk progesterone (P4) levels suggested that cows had started cyclic ovarian activity by 40 days postpartum, whereas only 25% had been observed in estrus. In the supplemented group, 80% of cows had started cyclic ovarian activity by the same time and 70% had been seen in estrus. Two types of cyclic activity in terms of progesterone changed were found. With Type I (normal), 50 and 80

  1. Comparison of claw health and milk yield in dairy cows on elastic or concrete flooring.

    PubMed

    Kremer, P V; Nueske, S; Scholz, A M; Foerster, M

    2007-10-01

    This article reports on the effects of elastic (rubber) flooring compared with concrete flooring on claw health and milk yield in dairy cows. Milk yield and activity data of 53 complete lactations from 49 cows were recorded by an automatic milking system in the University of Munich Livestock Center dairy herd. Cows were kept in a loose housing system on concrete-slatted or rubber-matted slatted flooring. Claws were trimmed and measured linearly in combination with claw lesion diagnosis 3 times during one lactation period (including the transition phase). An automatic milking system recorded milk yield and activity. The net horn growth of the claws increased on elastic flooring. Therefore, correct and frequent claw trimming is at least as important for claw health in dairy herds kept on rubber flooring as for those on concrete-slatted flooring. Cows housed on rubber had an increased incidence of sole ulcers. Sole hemorrhages (except for hemorrhages associated with sole ulcers) occurred less frequently on rubber than on concrete. Results concerning digital dermatitis were difficult to assess, because manual manure scraping on rubber required sprinkling the flooring twice daily, which additionally moistened the digital skin of the cows. This might explain the greater incidence of digital dermatitis on elastic flooring. The incidence of clinically lame cows did not differ between flooring types. Cows showed greater activity on rubber, most likely caused by the more comfortable walking surface compared with the concrete-slatted flooring. The greater activity may indicate better overall health of high-yielding dairy cows on rubber flooring. Milk yield, however, did not differ between flooring types.

  2. Examining the occurrence of residues of flunixin meglumine in cull dairy cows by use of the flunixin cull cow survey.

    PubMed

    Deyrup, Cynthia L; Southern, Kristal J; Cornett, Julie A; Shultz, Craig E; Cera, Deborah A

    2012-07-15

    To determine whether cull dairy cows with signs of certain clinical conditions, termed suspect, are more likely than healthy-appearing cull dairy cows to have violative concentrations of flunixin meglumine in their tissues at slaughter. Cross-sectional study. 961 cull dairy cows. Suspect cull dairy cows were selected from 21 beef slaughter establishments with a high production volume of dairy cows, and kidney and liver tissues were collected for screening. Kidney tissues were screened for antibiotics and sulfonamides with the fast antimicrobial screening test (FAST). Liver tissues were screened for flunixin meglumine with an ELISA, and quantitative analysis of ELISA-positive samples was performed with high-performance liquid chromatography. During the same time period, liver tissues from 251 healthy-appearing cull dairy cows were collected for the Food Safety and Inspection Service National Residue Program Scheduled Sampling Plan, but were screened only for flunixin meglumine. Of 710 suspect cull dairy cows, 50 (7.04%) had liver tissue flunixin concentrations higher than the flunixin tolerance concentration (0.125 ppm). Thirty-one of 168 (18.45%) FAST-positive and 19 of 542 (3.51%) FAST-negative suspect cull dairy cows had violative tissue flunixin concentrations. Two of the 251 (0.80%) healthy-appearing cull dairy cows had violative tissue flunixin concentrations. Suspect cull dairy cows, especially those that were also FAST positive, had a significantly higher incidence of violative tissue flunixin concentrations than healthy-appearing cull dairy cows at slaughter. Targeted sampling plans for flunixin meglumine in suspect dairy cows can help to support more efficient use of resources and further safeguard the nation's food supply.

  3. Effects of shampoo and water washing on hair cortisol concentrations.

    PubMed

    Hamel, Amanda F; Meyer, Jerrold S; Henchey, Elizabeth; Dettmer, Amanda M; Suomi, Stephen J; Novak, Melinda A

    2011-01-30

    Measurement of cortisol in hair is an emerging biomarker for chronic stress in human and nonhuman primates. Currently unknown, however, is the extent of potential cortisol loss from hair that has been repeatedly exposed to shampoo and/or water. Pooled hair samples from 20 rhesus monkeys were subjected to five treatment conditions: 10, 20, or 30 shampoo washes, 20 water-only washes, or a no-wash control. For each wash, hair was exposed to a dilute shampoo solution or tap water for 45 s, rinsed 4 times with tap water, and rapidly dried. Samples were then processed for cortisol extraction and analysis using previously published methods. Hair cortisol levels were significantly reduced by washing, with an inverse relationship between number of shampoo washes and the cortisol concentration. This effect was mainly due to water exposure, as cortisol levels following 20 water-only washes were similar to those following 20 shampoo treatments. Repeated exposure to water with or without shampoo appears to leach cortisol from hair, yielding values that underestimate the amount of chronic hormone deposition within the shaft. Collecting samples proximal to the scalp and obtaining hair washing frequency data may be valuable when conducting human hair cortisol studies. Copyright © 2010 Elsevier B.V. All rights reserved.

  4. Effects of Shampoo and Water Washing on Hair Cortisol Concentrations

    PubMed Central

    Hamel, Amanda F.; Meyer, Jerrold S.; Henchey, Elizabeth; Dettmer, Amanda M.; Suomi, Stephen J.; Novak, Melinda A.

    2010-01-01

    Background Measurement of cortisol in hair is an emerging biomarker for chronic stress in human and nonhuman primates. Currently unknown, however, is the extent of potential cortisol loss from hair that has been repeatedly exposed to shampoo and/or water. Methods Pooled hair samples from 20 rhesus monkeys were subjected to five treatment conditions: 10, 20, or 30 shampoo washes, 20 water-only washes, or a no-wash control. For each wash, hair was exposed to a dilute shampoo solution or tap water for 45 s, rinsed 4 times with tap water, and rapidly dried. Samples were then processed for cortisol extraction and analysis using previously published methods. Results Hair cortisol levels were significantly reduced by washing, with an inverse relationship between number of shampoo washes and the cortisol concentration. This effect was mainly due to water exposure, as cortisol levels following 20 water-only washes were similar to those following 20 shampoo treatments. Conclusions Repeated exposure to water with or without shampoo appears to leach cortisol from hair, yielding values that underestimate the amount of chronic hormone deposition within the shaft. Collecting samples proximal to the scalp and obtaining hair washing frequency data may be valuable when conducting human hair cortisol studies. PMID:21034727

  5. Monitoring cow activity and rumination time for an early detection of heat stress in dairy cow

    NASA Astrophysics Data System (ADS)

    Abeni, Fabio; Galli, Andrea

    2017-03-01

    The aim of this study was to explore the use of cow activity and rumination time by precision livestock farming tools as early alert for heat stress (HS) detection. A total of 58 Italian Friesian cows were involved in this study during summer 2015. Based on the temperature humidity index (THI), two different conditions were compared on 16 primiparous and 11 multiparous, to be representative of three lactation phases: early (15-84 DIM), around peak (85-154 DIM), and plateau (155-224 DIM). A separate dataset for the assessment of the variance partition included all the cows in the herd from June 7 to July 16. The rumination time (RT2h, min/2 h) and activity index (AI2h, bouts/2 h) were summarized every 2-h interval. The raw data were used to calculate the following variables: total daily RT (RTt), daytime RT (RTd), nighttime RT (RTn), total daily AI (AIt), daytime AI (AId), and nighttime AI (AIn). Either AIt and AId increased, whereas RTt, RTd, and RTn decreased with higher THI in all the three phases. The highest decrease was recorded for RTd and ranged from 49 % (early) to 45 % (plateau). The contribution of the cow within lactation phase was above 60 % of the total variance for AI traits and a share from 33.9 % (for RTt) to 54.8 % (RTn) for RT traits. These observations must be extended to different feeding managements and different animal genetics to assess if different thresholds could be identified to set an early alert system for the farmer.

  6. Prevalence of subclinical mastitis in Finnish dairy cows: changes during recent decades and impact of cow and herd factors.

    PubMed

    Hiitiö, Heidi; Vakkamäki, Johanna; Simojoki, Heli; Autio, Tiina; Junnila, Jouni; Pelkonen, Sinikka; Pyörälä, Satu

    2017-04-20

    The dairy industry has undergone substantial structural changes as intensive farming has developed during recent decades. Mastitis continues to be the most common production disease of dairy cows. Nationwide surveys of mastitis prevalence are useful in monitoring udder health of dairy herds and to study the impact of structural changes on the dairy industry. This survey on bovine subclinical mastitis was the first based on cow composite milk somatic cell count (SCC) data from the Finnish national health monitoring and milk recording database. A cow with composite milk SCC ≥200,000 cells/ml in at least one of the four test milkings during the year was considered to have subclinical mastitis and a cow with composite milk SCC ≥200,000 cells/ml in three or in all four test milkings during the year to have chronic subclinical mastitis. The aim of the study was to determine the prevalence of subclinical mastitis and chronic subclinical mastitis in Finland in 1991, 2001 and 2010 and to investigate cow and herd factors associated with elevated SCC. Prevalence of subclinical mastitis in Finland decreased over recent decades from 22.3% (1991) and 20.1% (2001) to 19.0% (2010). Prevalence of chronic subclinical mastitis was 20.4% in 1991, 15.5% in 2001 and 16.1% in 2010. The most significant cow and herd factors associated with subclinical mastitis or high milk SCC were increasing parity, Holstein breed, free-stalls with an automatic milking system and organic production. Milk SCC were highest from July to September. Main factors associated with chronic mastitis were increasing parity and Holstein breed. Prevalence of subclinical mastitis in Finland decreased over recent decades, the greatest change taking place during the first decade of the study. Prevalence of chronic subclinical mastitis significantly decreased from 1991. The most significant factors associated with both types of mastitis were increasing parity and Holstein breed, and for subclinical mastitis also

  7. Ink and Wash Painting for Children with Visual Impairment

    ERIC Educational Resources Information Center

    Shih, Chih-Ming; Chao, Hsin-Yi

    2010-01-01

    Five children with visual impairments received instruction in drawing, using ink and wash painting and calligraphy techniques. A special system developed by a blind Taiwanese Chinese calligrapher, Tsann-Cherng Liaw, was used to help the children orient and refine their work. Children's performance on simple drawing tasks was compared before and…

  8. Eating and rumination activities two weeks prepartum to one month postpartum in 100 healthy cows and cows with peripartum diseases.

    PubMed

    Braun, U; Buchli, H; Hässig, M

    2017-10-01

    Eating and rumination activities were investigated in 100 cows from 14 days prepartum to 30 days postpartum. All cows were clinically healthy at the start of the study. A pressure sensor incorporated into the noseband of a halter was used to record jaw movements, which allowed the quantification of the daily duration of eating and rumination, number of regurgitated cuds and number of chewing cycles per cud. The cows were retrospectively divided into 2 main groups healthy (n = 24) and ill cows (n = 76), and the latter were further divided into the following subgroups: cows with periparturient paresis (n = 12), retained placenta (n = 13), metritis (n = 17), primary ketosis (n = 19) and lameness (n = 6). Healthy cows had the shortest eating and rumination times on the day of calving; duration of eating decreased continually before and increased steadily after calving. In contrast, duration of rumination varied little except for a significant drop on the days of calving. Compared with healthy cows, eating times of ill cows were significantly shorter before and after calving and rumination time was reduced on days 2 to 4 postpartum. The duration of eating differed between healthy and ill cows before calving, and therefore the usefulness of eating and rumination variables for early recognition of periparturient diseases in cows requires further investigation.

  9. Fertilization and early embryonic development in heifers and lactating cows in summer and lactating and dry cows in winter.

    PubMed

    Sartori, R; Sartor-Bergfelt, R; Mertens, S A; Guenther, J N; Parrish, J J; Wiltbank, M C

    2002-11-01

    Two experiments in two seasons evaluated fertilization rate and embryonic development in dairy cattle. Experiment 1 (summer) compared lactating Holstein cows (n = 27; 97.3 +/- 4.1 d postpartum [dppl; 40.0 +/- 1.5 kg milk/d) to nulliparous heifers (n = 28; 11 to 17 mo old). Experiment 2 (winter) compared lactating cows (n = 27; 46.4 +/- 1.6 dpp; 45.9 +/- 1.4 kg milk/d) to dry cows (n = 26). Inseminations based on estrus included combined semen from four high-fertility bulls. Embryos and oocytes recovered 5 d after ovulation were evaluated for fertilization, embryo quality (1 = excellent to 5 = degenerate), nuclei/embryo, and accessory sperm. In experiment 1, 21 embryos and 17 unfertilized oocytes (UFO) were recovered from lactating cows versus 32 embryos and no UFO from heifers (55% vs. 100% fertilization). Embryos from lactating cows had inferior quality scores (3.8 +/- 0.4 vs. 2.2 +/- 0.3), fewer nuclei/embryo (19.3 +/- 3.7 vs. 36.8 +/- 3.0) but more accessory sperm (37.3 +/- 5.8 vs. 22.4 +/- 5.5/embryo) than embryos from heifers. Sperm were attached to 80% of UFO (17.8 +/- 12.1 sperm/UFO). In experiment 2, lactating cows yielded 36 embryos and 5 UFO versus 34 embryos and 4 UFO from dry cows (87.8 vs. 89.5% fertilization). Embryo quality from lactating cows was inferior to dry cows (3.1 +/- 0.3 vs. 2.2 +/- 0.3), but embryos had similar numbers of nuclei (27.2 +/- 2.7 vs. 30.6 +/- 2.1) and accessory sperm (42.0 +/- 9.4 vs. 36.5 +/- 6.3). From 53% of the flushings from lactating cows and 28% from dry cows, only nonviable embryos were collected. Thus, embryos of lactating dairy cows were detectably inferior to embryos from nonlactating females as early as 5 d after ovulation, with a surprisingly high percentage of nonviable embryos. In addition, fertilization rate was reduced only in summer, apparently due to an effect of heat stress on the oocyte.

  10. Examining the relationship between socio-economic status, WASH practices and wasting

    PubMed Central

    Raihan, Mohammad Jyoti; Farzana, Fahmida Dil; Sultana, Sabiha; Haque, Md Ahshanul; Rahman, Ahmed Shafiqur; Waid, Jillian L.; McCormick, Ben; Choudhury, Nuzhat; Ahmed, Tahmeed

    2017-01-01

    Childhood wasting is a global problem and is significantly more pronounced in low and middle income countries like Bangladesh. Socio Economic Status (SES) and Water, Sanitation and Hygiene (WASH) practices may be significantly associated with wasting. Most previous research is consistent about the role of SES, but the significance of WASH in the context of wasting remains ambiguous. The effect of SES and WASH on weight for length (WHZ) is examined using a Structural Equation Model (SEM) to explicitly describe the direct and indirect role of WASH in the context of SES.A nationally representative survey of 10,478 Bangladeshi children under 5 were examined. An expert defined SEM was used to construct latent variables for SES and WASH. The SEM included a direct pathway from SES to WHZ and an indirect pathway from SES to WHZ via WASH along with regression of relevant covariates on the outcome WHZ and the latent variables. Both SES (p<0.01) and WASH (p<0.05) significantly affect WHZ. SES (p<0.01) also significantly affects WASH. Other structural components showed that child’s age (p<0.01) affects WHZ and types of residence (p<0.01) affects SES. WASH practices at least partially mediate the association between SES and wasting status. WASH and SES are both significantly associated with WHZ. PMID:28278161

  11. Evaluation of conductive cooling of lactating dairy cows under controlled environmental conditions.

    PubMed

    Ortiz, X A; Smith, J F; Rojano, F; Choi, C Y; Bruer, J; Steele, T; Schuring, N; Allen, J; Collier, R J

    2015-03-01

    Cooling systems used to reduce heat stress in dairy operations require high energy, water usage, or both. Steady increases in electricity costs and reduction of water availability and an increase in water usage regulations require evaluation of passive cooling systems to cool cows and reduce use of water and electricity. A study was conducted to evaluate the use of heat exchangers buried 25 cm below the surface as components in a conductive system for cooling cows. Six cows were housed in environmentally controlled rooms with tie-stall beds, which were equipped with a heat exchanger and filled with 25 cm of either sand or dried manure. Beds were connected to supply and return lines and individually controlled. Two beds (one per each kind of bedding material) constituted a control group (water off), and the other 4 (2 sand and 2 dried manure) used water at 7°C passing through the heat exchangers (water on). The experiment was divided in 2 periods of 40 d, and each period involved 3 repetitions of 3 different climates (hot and dry, thermo neutral, and hot and humid). Each cow was randomly assigned to a different treatment after each repetition was over. Sand bedding remained cooler than dried manure bedding in all environments and at all levels of cooling (water on or off). Bed temperatures were lower and heat flux higher during the bed treatment with sand and water on. We also detected a reduction in core body temperatures, respiration rates, rectal temperatures, and skin temperatures of those cows during the sand and water on treatment. Feed intake and milk yield numerically increased during the bed treatment with sand and water on for all climates. No major changes were observed in the lying time of cows or the composition of the milk produced. We conclude that use of heat exchangers is a viable adjunct to systems that employ fans, misters, and evaporative cooling methods to mitigate effects of heat stress on dairy cows. Sand was superior to dried manure as a

  12. A novel washing algorithm for underarm stain removal

    NASA Astrophysics Data System (ADS)

    Acikgoz Tufan, H.; Gocek, I.; Sahin, U. K.; Erdem, I.

    2017-10-01

    After contacting with human sweat which comprise around 27% sebum, anti-perspirants comprising aluminium chloride or its compounds form a jel-like structure whose solubility in water is very poor. In daily use, this jel-like structure closes sweat pores and hinders wetting of skin by sweat. However, when in contact with garments, they form yellowish stains at the underarm of the garments. These stains are very hard to remove with regular machine washing. In this study, first of all, we focused on understanding and simulating such stain formation on the garments. Two alternative procedures are offered to form jel-like structures. On both procedures, commercially available spray or deo-stick type anti-perspirants, standard acidic and basic sweat solutions and artificial sebum are used to form jel-like structures, and they are applied on fabric in order to get hard stains. Secondly, after simulation of the stain on the fabric, we put our efforts on developing a washing algorithm specifically designed for removal of underarm stains. Eight alternative washing algorithms are offered with varying washing temperature, amounts of detergent, and pre-stain removal procedures. Better algorithm is selected by comparison of Tristimulus Y values after washing.

  13. Associations of insulin resistance later in lactation on fertility of dairy cows.

    PubMed

    Baruselli, P S; Vieira, L M; Sá Filho, M F; Mingoti, R D; Ferreira, R M; Chiaratti, M R; Oliveira, L H; Sales, J N; Sartori, R

    2016-07-01

    The challenge of getting dairy cows pregnant during early lactation is a well-described, worldwide problem. However, specifically in farms with poor reproductive, nutritional, and environmental conditions/management, a low pregnancy rate during early lactation is followed inevitably by an increased number of nonpregnant cows after 150 days in milk, with even more difficulties to achieve pregnancy. Therefore, several studies were designed to understand and develop strategies to mitigate reduced fertility of cows during late lactation. Experiments were performed under tropical regions to determine metabolic status during lactation and association of stage of lactation on oocyte quality and fertility. Lactating cows with extended days not pregnant (e.g.,>150 days in milk) often had systemic metabolic alterations, including development of peripheral insulin resistance and various oocyte alterations, including reduced expression of genes encoding glucose transport proteins, reduced amounts of mtDNA, increased expression of mitochondria-related genes, and increased expression of apoptosis-related genes. Additionally, in vitro embryo production and pregnancy per AI were lower in late- versus early-lactation cows in some but not all studies. Notwithstanding, when a normal embryo was transferred to a cow in late lactation, the pregnancy per transfer was reasonable, reinforcing the assertion that fertility problems in late-lactation cows may be associated with oocyte quality, fertilization, and/or failure of early embryo development. In conclusion, insulin resistance may reduce oocyte competence and consequently fertility in late-lactation dairy cows. Copyright © 2016 Elsevier Inc. All rights reserved.

  14. Endotoxins in cotton: washing effects and size distribution

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Olenchock, S.A.; Mull, J.C.; Jones, W.G.

    1983-01-01

    Endotoxin contamination was measured in washed and unwashed cottons from three distinct growing areas, California, Mississippi, and Texas. The data show differences in endotoxin contamination based upon the geographic source of the cotton. It is also shown that washing bulk cotton before the carding process results in lower endotoxin in the cotton dust. Washing conditions can affect the endotoxin levels, and all size fractions of the airborne dust contain quantifiable endotoxin contamination. Endotoxin analyses provide a simple and reliable method for monitoring the cleanliness of cotton or airborne cotton dusts.

  15. Effects of different dosages of propylene glycol in dry cows and cows in early lactation.

    PubMed

    Maurer, Michaela; Peinhopf, Walter; Gottschalk, Jutta; Einspanier, Almut; Koeller, Gabor; Wittek, Thomas

    2017-11-01

    In this Research Paper we hypothesised that the temporary insulin resistance seen during the transition period in dairy cows may cause significant differences in the efficacy of PG at different sampling periods and that in some cases this effect will be dose dependent. Eighty four sampling sets were generated by studying 7 multiparous Holstein cows repeatedly at 4 sampling periods of 3 d length (dry cows: days 40, 39 and 38 antepartum; close up cows: days 10, 9 and 8 antepartum; fresh cows: days 3, 4 and 5 post-partum; lactating cows: days 38, 39 and 40 post-partum). On each of these days 3 h after morning feeding propylene glycol was drenched in different dosages of 100, 300 or 500 ml once per day (cross over study). The different doses were applied in an alternating order (Latin square). Blood samples were taken before, every 30 min up to 4 h, after 6 and 12 h after PG application. Following parameters have been measured: insulin, non-esterified fatty acids (NEFA), betahydroxybutyrate (BHB), bilirubin, cholesterol, potassium, aspartate aminotransferase (AST) and glutamate dehydrogenase (GLDH). Revised Quantitative Insulin Sensitivity Check Index (RQUICKI) was calculated. It was found that glucose, insulin, NEFA, BHB, bilirubin and potassium concentrations were influenced differently by the three defined dosages of propylene glycol at four different sampling periods. Whereas RQUICKI, cholesterol, AST and GLDH did not differ between the sampling periods and treatments. The major results of the study are that the effect of PG is dose-dependent and that the effect of PG is depending on the time of application according to calving. It can be concluded that in fresh cows higher dosages are necessary to provoke similar effects in comparison to dry, close up and lactating cows. Although the study did not compare to topdressing of PG from the results it is reasonable to believe that bolus application of a specific PG volume is necessary to provoke the effect.

  16. On-line casein micelle disruption for downstream purification of recombinant human myelin basic protein produced in the milk of transgenic cows.

    PubMed

    Al-Ghobashy, Medhat A; Williams, Martin A K; Brophy, Brigid; Laible, Götz; Harding, David R K

    2009-06-01

    Downstream purification of a model recombinant protein (human myelin basic protein) from milk of transgenic cows is described. The recombinant protein was expressed as a His tagged fusion protein in the milk of transgenic cows and was found associated with the casein micellar phase. While difficulties in obtaining good recoveries were found when employing conventional micelle disruption procedures, direct capture using the cation exchanger SP Sepharose Big Beads was found successful in the extraction of the recombinant protein. Early breakthrough suggested a slow release of the recombinant protein from the micelles and dictated micelle disruption in order to obtain good yields. A new approach for deconstruction of the calcium core of the casein micelles, employing the interaction between the micellar calcium and the active sites of the cation exchanger resin was developed. Milk samples were loaded to the column in aliquots with a column washing step after each aliquot. This sequential loading approach successfully liberated the recombinant protein from the micelles and was found superior to the conventional sample loading approach. It increased the recovery by more than 25%, reduced fouling due to milk components and improved the column hydrodynamic properties as compared to the conventional sample loading approach. Hardware and software modifications to the chromatography system were necessary in order to keep the whole process automated. A second purification step using a Ni2+ affinity column was used to isolate the recombinant protein at purity more than 90% and a recovery percentage of 78%.

  17. Endocannabinoids as endometrial inflammatory markers in lactating Holstein cows.

    PubMed

    Bonsale, R; Seyed Sharifi, R; Dirandeh, E; Hedayat, N; Mojtahedin, A; Ghorbanalinia, M; Abolghasemi, A

    2018-06-01

    The objective of this study was to consider endocannabinoid system as inflammatory markers in bovine endometrium to better understand the role of this system in regulating many of the functions that are related to inflammatory condition. At day 26 post-partum, fourteen cows were divided into two groups depending on the inflammatory condition: 1- subclinical endometritis (n = 7, with purulent or mucopurulent uterine discharge detectable in the vagina) and 2- healthy (n = 7, No (muco)) purulent discharge. Blood samples were collected at 26 and 30 days relative to calving to determine plasma tumour necrosis factor (TNF) and lipopolysaccharide-binding protein (LBP) concentrations; moreover, uterine biopsy was carried out on day 26 post-partum to measure mRNA abundance of TNF, interleukin-1B (IL1B), interleukin-6 (IL-6), C-X-C motif chemokine ligand 8 (CXCL8), endocannabinoid receptor (CNR2), N-acyl phosphatidylethanolamine phospholipase D (NAPEPLD), fatty acid amide hydrolase (FAAH), N-acylethanolamine acid amidase (NAAA) and monoglyceride lipase (MGLL) by real-time PCR. Results showed mean plasma concentrations of TNF and LBP were lower in healthy cows compared to subclinical endometritis cows (p < .05). Relative mRNA expression for NAAA and FAAH was decreased (p < .05), and relative mRNA expression for CNR2 and NAPEPLD increased in cows with subclinical endometritis compared to healthy cows. In conclusion, relative mRNA expression of TNF, IL1B and CXCL8 and plasma concentration of LBP increased during inflammatory condition along with decreased endocannabinoids hydrolyzing enzyme (NAAA and FAAH), increased enzymes that synthesize endocannabinoids (NAPEPLD) and relative gene expression of the endocannabinoid receptor; together, these contribute to increased endocannabinoids levels during inflammation. Overall, we provide evidence that endocannabinoid system is altered in endometrium tissue during inflammation through increased mRNA expression of CNR2 and

  18. Improving understanding of the underlying physical process of sediment wash-off from urban road surfaces

    NASA Astrophysics Data System (ADS)

    Muthusamy, Manoranjan; Tait, Simon; Schellart, Alma; Beg, Md Nazmul Azim; Carvalho, Rita F.; de Lima, João L. M. P.

    2018-02-01

    Among the urban aquatic pollutants, the most common is sediment which also acts as a transport medium for many contaminants. Hence there is an increasing interest in being able to better predict the sediment wash-off from urban surfaces. The exponential wash-off model is the most widely used method to predict the sediment wash-off. Although a number of studies proposed various modifications to the original exponential wash-off equation, these studies mostly looked into one parameter in isolation thereby ignoring the interactions between the parameters corresponding to rainfall, catchment and sediment characteristics. Hence in this study we aim (a) to investigate the effect of rainfall intensity, surface slope and initial load on wash-off load in an integrated and systematic way and (b) to subsequently improve the exponential wash-off equation focusing on the effect of the aforementioned three parameters. A series of laboratory experiments were carried out in a full-scale setup, comprising of a rainfall simulator, a 1 m2 bituminous road surface, and a continuous wash-off measuring system. Five rainfall intensities ranging from 33 to 155 mm/h, four slopes ranging from 2 to 16% and three initial loads ranging from 50 to 200 g/m2 were selected based on values obtained from the literature. Fine sediment with a size range of 300-600 μm was used for all of the tests. Each test was carried out for one hour with at least 9 wash-off samples per test collected. Mass balance checks were carried out for all the tests as a quality control measure to make sure that there is no significant loss of sand during the tests. Results show that the washed off sediment load at any given time is proportional to initial load for a given combination of rainfall intensity and surface slope. This indicates the importance of dedicated modelling of build-up so as to subsequently predict wash-off load. It was also observed that the maximum fraction that is washed off from the surface increases

  19. Detection of Vaccinia virus in blood and faeces of experimentally infected cows.

    PubMed

    Guedes, M I M C; Rehfeld, I S; de Oliveira, T M L; Assis, F L; Matos, A C D; Abrahão, J S; Kroon, E G; Lobato, Z I P

    2013-12-01

    Bovine vaccinia (BV), a zoonosis caused by Vaccinia virus (VACV), affects dairy cattle and milkers, causing economic, veterinary and human health impacts. Despite such impacts, there are no experimental studies about the pathogenesis of BV in cows to assess whether there is a systemic spread of the virus and whether there are different ways of VACV shedding. Trying to answer some of these questions, a study was proposed using experimental inoculation of VACV in cows. All experimentally infected cows developed lesions compatible with VACV infection in cattle. Two of the six animals presented VACV DNA in blood and faecal samples, starting at the 2nd and the 3rd day post-infection (d.p.i.), respectively, and lasting until the 36th d.p.i., in an intermittent way. This study provides new evidence that VACV can be detected in blood and faeces of infected cows, suggesting that BV could be a systemic disease, and also bringing new information about the epidemiology and pathogenesis of BV. © 2012 Blackwell Verlag GmbH.

  20. Is mad cow disease caused by a bacteria?

    PubMed

    Broxmeyer, L

    2004-01-01

    England, where one quarter of all tuberculous meningitis victims suffered from Mycobacterium bovis infection. And Harley's study showed pathology identical to "mad cow" from systemic M. bovis in cattle, causing a tuberculous spongiform encephalitis. In addition to M. bovis, Mycobacterium avium subspecies paratuberculosis (fowl tuberculosis) causes Johne's disease, a problem known and neglected in cattle and sheep for almost a century, and rapidly emerging as the disease of the new millennium. Not only has M. paratuberculosis been found in human Crohn's disease, but both Crohn's and Johne's both cross-react with the antigens of cattle paratuberculosis. Furthermore, central neurologic manifestations of Crohn's disease are not unknown. There is no known disease which better fits into what is occurring in Mad Cow and the spongiform enchephalopathies than bovine tuberculosis and its blood-brain barrier penetrating, virus-like, cell-wall-deficient forms. It is for these reasons that future research needs to be aimed in this direction. Copyright 2004 Elsevier Ltd.

  1. Maintenance energy requirements of beef cows and relationship with cow and calf performance, metabolic hormones, and functional proteins.

    PubMed

    Cooper-Prado, M J; Long, N M; Davis, M P; Wright, E C; Madden, R D; Dilwith, J W; Bailey, C L; Spicer, L J; Wettemann, R P

    2014-08-01

    Gestating Angus, nonlactating, spring-calving cows were used to determine variation in maintenance energy requirements (MR); to evaluate the relationship among MR and cow and calf performance, plasma concentrations of IGF-I, T4, glucose, insulin, and ruminal temperature; and to describe the LM proteome and evaluate protein abundance in cows with different MR. Cows (4 to 7 yr of age) with a BCS of 5.0 ± 0.2 and BW of 582 ± 37 kg in the second to third trimester of gestation were studied in 3 trials (trial 1, n = 23; trial 2, n = 32; trial 3, n = 38). Cows were individually fed a complete diet in amounts to meet predicted MR (Level 1 Model of NRC), and feed intake was adjusted weekly until constant BW was achieved for at least 21 d (maintenance). Cows were classified on the basis of MR as low (>0.5 SD less than mean, LMR), moderate (±0.5 SD of mean, MMR), or high (>0.5 SD more than mean, HMR) MR. Blood samples were taken at maintenance and at 2 mo postpartum in trial 2. Muscle biopsies were taken from LMR and HMR after cows consumed actual MR for 28 d (trial 2) or 21 d (trial 3). Proteins from LM were separated by 2-dimensional difference gel electrophoresis and were identified, and abundance was quantified and compared. The greatest differences in MR between cows were 29%, 24%, and 25% in trials 1, 2, and 3, respectively. Daily MR (NEm, kcal·BW(-0.75)·d(-1)) averaged 89.2 ± 6.3, 93.0 ± 4.9, and 90.4 ± 4.6 in trials 1, 2, and 3, respectively. Postpartum BW and BCS, calf birth and weaning weights, postpartum luteal activity, and ruminal temperature were not influenced by MR of the cows. Concentrations of IGF-I were greater (P = 0.001) in plasma of MMR compared with LMR cows consuming predicted MR diets, and MR was negatively correlated with concentrations of IGF-I in plasma (r = -0.38; P = 0.05) at 2 mo postpartum. A total of 103 proteins were isolated from LM; 52 gene products were identified. Abundance of specific proteins in the LM was not influenced (P > 0

  2. Different Blood Cell-Derived Transcriptome Signatures in Cows Exposed to Vaccination Pre- or Postpartum

    PubMed Central

    Weikard, Rosemarie; Demasius, Wiebke; Hadlich, Frieder; Kühn, Christa

    2015-01-01

    Periparturient cows have been found to reveal immunosuppression, frequently associated with increased susceptibility to uterine and mammary infections. To improve understanding of the causes and molecular regulatory mechanisms accounting for this phenomenon around calving, we examined the effect of an antigen challenge on gene expression modulation on cows prior to (BC) or after calving (AC) using whole transcriptome sequencing (RNAseq). The transcriptome analysis of the cows’ blood identified a substantially higher number of loci affected in BC cows (2,235) in response to vaccination compared to AC cows (208) and revealed a divergent transcriptional profile specific for each group. In BC cows, a variety of loci involved in immune defense and cellular signaling processes were transcriptionally activated, whereas protein biosynthesis and posttranslational processes were tremendously impaired in response to vaccination. Furthermore, energy metabolism in the blood cells of BC cows was shifted from oxidative phosphorylation to the glycolytic system. In AC cows, the number and variety of regulated pathways involved in immunomodulation and maintenance of immnunocompetence are considerably lower after vaccination, and upregulation of arginine degradation was suggested as an immunosuppressive mechanism. Elevated transcript levels of erythrocyte-specific genes involved in gas exchange processes were a specific transcriptional signature in AC cows pointing to hematopoiesis activation. The divergent and substantially lower magnitude of transcriptional modulation in response to vaccination in AC cows provides evidence for a suppressed immune capacity of early lactating cows on the molecular level and demonstrates that an efficient immune response of cows is related to their physiological and metabolic status. PMID:26317664

  3. Modification of immune responses and digestive system microbiota of lactating dairy cows by feeding Bovamine(R)

    USDA-ARS?s Scientific Manuscript database

    We evaluated the immune modulatory effects as well as effects on productivity of Bovamine® (Lactobacillus acidophilus strain NP51 and Probionibacterium freudenreichii) fed to Holstein and Jersey dairy cows during late lactation (average DIM = 202.44 days on wk-0). Cows were randomized to treatment g...

  4. Suitability of feeding and chewing time for estimation of feed intake in dairy cows.

    PubMed

    Pahl, C; Hartung, E; Grothmann, A; Mahlkow-Nerge, K; Haeussermann, A

    2016-09-01

    Monitoring of feeding and rumination behaviour can provide useful information for dairy herd management. The feeding behaviour of dairy cows can be recorded by different techniques, such as video cameras, weighing troughs or chewing sensors. Among feeding characteristics, individual feed intake of cows is of utmost interest, but as weighing troughs have high space and cost requirements they are used primarily in research studies. The objective of the present study was to evaluate whether records on feeding time or chewing activity or a combination of both contain enough information to estimate feed intake with sufficient accuracy. Feed intake and feeding time per cow were recorded by means of weighing troughs. Concurrently, chewing activity of seven cows was recorded by MSR-ART pressure sensors during five to eight measuring days per cow. Feeding and chewing behaviour were evaluated in time slots (1 min) and additionally assigned to feeding bouts for further analysis. The 1 min time slots were classified into feeding/no feeding or chewing/no chewing by the two systems, and agreement was found in 92.2% of the records. On average, cows spent 270±39 min/day at the feeding troughs and chewed 262±48 min/day. The average fresh matter intake (FMI) was 49.6±5.1 kg/day. Feed intake was divided into 9.7 bouts/day during which cows fed in average 27.8±21.7 min/bout and chewed 27.0±23.1 min/bout. The correlation between FMI and feeding time was r=0.891 and between FMI and chewing time r=0.780 overall cows. Hence, both systems delivered suitable information for estimating feed intake.

  5. Hand washing frequencies and procedures used in retail food services.

    PubMed

    Strohbehn, Catherine; Sneed, Jeannie; Paez, Paola; Meyer, Janell

    2008-08-01

    Transmission of viruses, bacteria, and parasites to food by way of improperly washed hands is a major contributing factor in the spread of foodborne illnesses. Field observers have assessed compliance with hand washing regulations, yet few studies have included consideration of frequency and methods used by sectors of the food service industry or have included benchmarks for hand washing. Five 3-h observation periods of employee (n = 80) hand washing behaviors during menu production, service, and cleaning were conducted in 16 food service operations for a total of 240 h of direct observation. Four operations from each of four sectors of the retail food service industry participated in the study: assisted living for the elderly, childcare, restaurants, and schools. A validated observation form, based on 2005 Food Code guidelines, was used by two trained researchers. Researchers noted when hands should have been washed, when hands were washed, and how hands were washed. Overall compliance with Food Code recommendations for frequency during production, service, and cleaning phases ranged from 5% in restaurants to 33% in assisted living facilities. Procedural compliance rates also were low. Proposed benchmarks for the number of times hand washing should occur by each employee for each sector of food service during each phase of operation are seven times per hour for assisted living, nine times per hour for childcare, 29 times per hour for restaurants, and 11 times per hour for schools. These benchmarks are high, especially for restaurant employees. Implementation would mean lost productivity and potential for dermatitis; thus, active managerial control over work assignments is needed. These benchmarks can be used for training and to guide employee hand washing behaviors.

  6. Productivity of different cow genetic groups in dual-purpose cattle production systems in south-eastern Mexico.

    PubMed

    Magana, J G; Tewolde, A; Anderson, S; Segura, J C

    2006-01-01

    The objective was to evaluate the effect of cow genetic group, nutritional level and their interaction on some economically important traits of dual-purpose herds managed under field conditions. Nine herds were monitored during a production cycle in Yucatan, Mexico. Herds were grouped into four nutritional levels (NL) based on the metabolizable energy (ME) apparently available on pasture, nutritional management, and milk production. Cows were classified into three genetic groups (GG): low (< or = 25%), middle (25-75%) and high (> or = 75%) graded for Bos taurus inheritance. Total milk sold (TMS), days in milk (DIM), TMS adjusted to DIM within each NL (TMSA), body condition score (BCS) at calving, changes of BCS during lactation (CBCS), calf weaning weight (WW), age at weaning (AW), kg of calf weaned per cow (KWC) and calf mortality were studied. The statistical model included the fixed effects of NL, GG, month of calving (MC), parity number (PN) and BCS at calving and GG x NL interaction. The effects of NL, GG, MC, PN and GG x NL were significant (p < 0.05) for TMS, KWC. As expected, TMS increased with NL from 562.4 +/- 106 kg for NL1 to 2366.3 +/- 100.1 kg for NL4. KWC was greatest for NL2 (138.6 kg) followed by NL1 (135 kg); the lowest KWC corresponded to NL4 (96.0 kg) (p < 0.05). TMS values for the middle (1727 +/- 94.7 kg) and the high graded GG (1603.5 +/- 83.5 kg) were twice those for the low graded GG cows (828.5 +/- 95 kg) (p < 0.05). KWC was also higher for the middle graded group (152.8 kg) than for the low or (104 kg) or the high graded GG (118 kg) (p < 0.05). With better nutrition cows of all GG improved their milk performance but not the calf traits. CBCS was negative for all GG. The highest BCS lost was for cows in NL1 and NL2 and for cows in the high graded GG (p < 0.05).

  7. Quality characteristics, chemical composition, and sensory properties of butter from cows on pasture versus indoor feeding systems.

    PubMed

    O'Callaghan, Tom F; Faulkner, Hope; McAuliffe, Stephen; O'Sullivan, Maurice G; Hennessy, Deirdre; Dillon, Pat; Kilcawley, Kieran N; Stanton, Catherine; Ross, R Paul

    2016-12-01

    This study evaluated the effects of 3 widely practiced cow feeding systems in the United States, Europe, and Southern Hemisphere regions on the characteristics, quality, and consumer perception of sweet cream butter. Fifty-four multiparous and primiparous Friesian cows were divided into 3 groups (n=18) for an entire lactation. Group 1 was housed indoors and fed a total mixed ration diet (TMR) of grass silage, maize silage, and concentrates; group 2 was maintained outdoors on perennial ryegrass-only pasture (GRS); and group 3 was maintained outdoors on a perennial ryegrass/white clover pasture (CLV). Mid-lactation butter was manufactured in triplicate with milk from each group in June 2015 (137±7d in milk) and was analyzed over a 6-mo storage period at 5°C for textural and thermal properties, fatty acid composition, sensory properties, and volatile compounds. The nutritional value of butters was improved by pasture feeding, and butter from pasture-fed cows had significantly lower thrombogenicity index scores compared with butters from TMR-fed cows. In line with these results, pasture-derived milks (GRS and CLV) produced butter with significantly higher concentrations of conjugated linoleic acid (cis-9,trans-11) and trans-β-carotene than TMR butter. Alterations in the fatty acid composition of butter contributed to significant differences in textural and thermal properties of the butters. Total mixed ration-derived butters had significantly higher hardness scores at room temperature than those of GRS and CLV. Onset of crystallization for TMR butters also occurred at significantly higher temperatures compared with pasture butters. Volatile analysis of butter by gas chromatography-mass spectrometry identified 25 compounds present in each of the butters, 5 of which differed significantly based on feeding system, including acetone, 2-butanone, 1-pentenol, toluene, and β-pinene. Toluene was very significantly correlated with pasture-derived butter. Sensory analysis

  8. Humic substances as a washing agent for Cd-contaminated soils.

    PubMed

    Meng, Fande; Yuan, Guodong; Wei, Jing; Bi, Dongxue; Ok, Yong Sik; Wang, Hailong

    2017-08-01

    Cost-effective and eco-friendly washing agents are in demand for Cd contaminated soils. Here, we used leonardite-derived humic substances to wash different types of Cd-contaminated soils, namely, a silty loam (Soil 1), a silty clay loam (Soil 2), and a sandy loam (Soil 3). Washing conditions were investigated for their effects on Cd removal efficiency. Cadmium removal was enhanced by a high humic substance concentration, long washing time, near neutral pH, and large solution/soil ratio. Based on the tradeoff between efficiency and cost, an optimum working condition was established as follows: humic substance concentration (3150 mg C/L), solution pH (6.0), washing time (2 h) and a washing solution/soil ratio (5). A single washing removed 0.55 mg Cd/kg from Soil 1 (1.33 mg Cd/kg), 2.32 mg Cd/kg from Soil 2 (6.57 mg Cd/kg), and 1.97 mg Cd/kg from Soil 3 (2.63 mg Cd/kg). Cd in effluents was effectively treated by adding a small dose of calcium hydroxide, reducing its concentration below the discharge limit of 0.1 mg/L in China. Being cost-effective and safe, humic substances have a great potential to replace common washing agents for the remediation of Cd-contaminated soils. Besides being environmentally benign, humic substances can improve soil physical, chemical, and biological properties. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Lameness Detection in Dairy Cows: Part 2. Use of Sensors to Automatically Register Changes in Locomotion or Behavior

    PubMed Central

    Van Nuffel, Annelies; Zwertvaegher, Ingrid; Van Weyenberg, Stephanie; Pastell, Matti; Thorup, Vivi M.; Bahr, Claudia; Sonck, Bart; Saeys, Wouter

    2015-01-01

    Simple Summary As lame cows produce less milk and tendto have other health problems, finding and treating lame cows is very importantfor farmers. Sensors that measure behaviors associated with lameness in cowscan help by alerting the farmer of those cows in need of treatment. This reviewgives an overview of sensors for automated lameness detection and discussessome practical considerations for investigating and applying such systems inpractice. Abstract Despite the research on opportunities toautomatically measure lameness in cattle, lameness detection systems are notwidely available commercially and are only used on a few dairy farms. However, farmers need to be aware of the lame cows in their herds in order treat themproperly and in a timely fashion. Many papers have focused on the automatedmeasurement of gait or behavioral cow characteristics related to lameness. Inorder for such automated measurements to be used in a detection system, algorithms to distinguish between non-lame and mildly or severely lame cowsneed to be developed and validated. Few studies have reached this latter stageof the development process. Also, comparison between the different approachesis impeded by the wide range of practical settings used to measure the gait or behavioralcharacteristic (e.g., measurements during normal farming routine or duringexperiments; cows guided or walking at their own speed) and by the differentdefinitions of lame cows. In the majority of the publications, mildly lame cowsare included in the non-lame cow group, which limits the possibility of alsodetecting early lameness cases. In this review, studies that used sensortechnology to measure changes in gait or behavior of cows related to lamenessare discussed together with practical considerations when conducting lamenessresearch. In addition, other prerequisites for any lameness detection system onfarms (e.g., need for early detection, real-time measurements) are discussed. PMID:26479390

  10. Monitoring cow activity and rumination time for an early detection of heat stress in dairy cow.

    PubMed

    Abeni, Fabio; Galli, Andrea

    2017-03-01

    The aim of this study was to explore the use of cow activity and rumination time by precision livestock farming tools as early alert for heat stress (HS) detection. A total of 58 Italian Friesian cows were involved in this study during summer 2015. Based on the temperature humidity index (THI), two different conditions were compared on 16 primiparous and 11 multiparous, to be representative of three lactation phases: early (15-84 DIM), around peak (85-154 DIM), and plateau (155-224 DIM). A separate dataset for the assessment of the variance partition included all the cows in the herd from June 7 to July 16. The rumination time (RT2h, min/2 h) and activity index (AI2h, bouts/2 h) were summarized every 2-h interval. The raw data were used to calculate the following variables: total daily RT (RTt), daytime RT (RTd), nighttime RT (RTn), total daily AI (AIt), daytime AI (AId), and nighttime AI (AIn). Either AIt and AId increased, whereas RTt, RTd, and RTn decreased with higher THI in all the three phases. The highest decrease was recorded for RTd and ranged from 49 % (early) to 45 % (plateau). The contribution of the cow within lactation phase was above 60 % of the total variance for AI traits and a share from 33.9 % (for RTt) to 54.8 % (RTn) for RT traits. These observations must be extended to different feeding managements and different animal genetics to assess if different thresholds could be identified to set an early alert system for the farmer.

  11. Factors affecting reproductive performance of dairy cows.

    PubMed

    Coleman, D A; Thayne, W V; Dailey, R A

    1985-07-01

    We conducted two studies to determine how herd management practices and traits of individual cows affect performance of the herd and of the cow within a herd. Management practices, reproductive performance of the herd, and relationships between management and reproductive performance were characterized on 83 dairy farms with 7596 cows. Data included 21 management variables (e.g., facilities, herd health program, estrous detection program) and 8 performance variables obtained from Dairy Herd Improvement or unofficial records (e.g., size of herd, production, days open). Although varying among herds, annual average herd incidences of reproductive disorders and reproductive performance were similar to those reported. Managerial practices influenced incidences of retained placenta and uterine infection, days open of cows not bred and of all cows, services per conception, and percentages of herd open more than 100 days and culled for low production. Veterinarian was the most consistent variable influencing herd reproductive performance. Data also were collected from production and lifetime records of 2532 cows in 19 herds. Reproductive performance was affected by season of calving, production, maturity, and reproductive disorders. Several cows with extremely poor reproductive records were maintained.

  12. Importance of secondary damage in downer cows.

    PubMed

    Poulton, P J; Vizard, A L; Anderson, G A; Pyman, M F

    2016-05-01

    To investigate the relative importance in downer cows of the primary cause of recumbency in comparison with secondary complications. Downer dairy cows were monitored during their recumbency under field conditions in South Gippsland, Victoria, Australia. The cause of the original recumbency of the 218 cows was determined and secondary damage, status on day 7 and final outcome were recorded. Some type of secondary damage was found in 183/218 (84%) cows, of which 173/218 (79%) had damage deemed to be clinically important. By day 7, 52 (24%) had recovered and 69 (32%) eventually recovered. Of the 149 (68%) cows that were euthanased or died, 23 (15%) were deemed to have been lost solely from the primary cause, 107 (72%) from secondary damage and 19 (13%) from a combination of both. There was no difference in recovery among the five broad groups of causes of primary recumbency. Secondary damage was very common and presented in a large variety of ways, with many cows having multiple types of secondary damage concurrently. For most cows the secondary damage was more important than the initial primary damage in determining their fate. © 2016 Australian Veterinary Association.

  13. Heterosis and direct effects for Charolais-sired calf weight and growth, cow weight and weight change, and ratios of cow and calf weights and weight changes across warm season lactation in Romosinuano, Angus, and F cows in Arkansas.

    PubMed

    Riley, D G; Burke, J M; Chase, C C; Coleman, S W

    2016-01-01

    The use of Brahman in cow-calf production offers some adaptation to the harsh characteristics of endophyte-infected tall fescue. Criollo breeds, such as the Romosinuano, may have similar adaptation. The objectives were to estimate genetic effects in Romosinuano, Angus, and crossbred cows for their weights, weights of their calves, and ratios (calf weight:cow weight and cow weight change:calf weight gain) across lactation and to assess the influence of forage on traits and estimates. Cows ( = 91) were bred to Charolais bulls after their second parity. Calves ( = 214) were born from 2006 to 2009. Cows and calves were weighed in early (April and June), mid- (July), and late lactation (August and October). Animal was a random effect in analyses of calf data; sire was random in analyses of cow records and ratios. Fixed effects investigated included calf age, calf sex, cow age-year combinations, sire breed of cow, dam breed of cow, and interactions. Subsequent analyses evaluated the effect of forage grazed: endophyte-free or endophyte-infected tall fescue. Estimates of maternal heterosis for calf weight ranged from 9.3 ± 4.3 to 15.4 ± 5.7 kg from mid-lactation through weaning ( < 0.05). Romosinuano direct effects (of the cow) were -6.8 ± 3.0 and -8.9 ± 4.2 kg for weights recorded in April and June. Calf weights and weight gains from birth were greater ( < 0.05) for calves of cows grazing endophyte-free tall fescue except in mid-summer. Cow weight change from April to each time was negative for Angus cows and lower ( < 0.05) than other groups. Cows grazing endophyte-free tall fescue were heavier ( < 0.05) at all times but had more weight loss in late lactation. Angus cows had the lowest ( < 0.05) ratios (negative) of cow weight change:calf weight gain, indicating an energy-deficit condition. Cows grazing endophyte-free tall fescue had more negative ( < 0.05) values for this trait but not in early lactation ( < 0.05). Estimates of heterosis ranged from 12.8 ± 9.5 to

  14. Treatment of Wastewater From Car Washes Using Natural Coagulation and Filtration System

    NASA Astrophysics Data System (ADS)

    Al-Gheethi, A. A.; Mohamed, R. M. S. R.; Rahman, M. A. A.; Johari, M. R.; Kassim, A. H. M.

    2016-07-01

    Wastewater generated from carwash is one of the main wastewater resources, which contribute effectively in the increasing of environmental contamination due to the chemical characteristics of the car wastes. The present work aimed to develop an integrated treatment system for carwash wastewater based on coagulation and flocculation using Moringa oleifera and Ferrous Sulphate (FeSO4.7H2O) as well as natural filtration system. The carwash wastewater samples were collected from carwash station located at Parit Raja, Johor, Malaysia. The treatment system of car wash wastewater was designed in the lab scale in four stages included, aeration, coagulation and flocculation, sedimentation and filtration. The coagulation and flocculation unit was carried out using different dosage (35, 70, 105 and 140 mg L-1) of M. oleifera and FeSO4.7H2O, respectively. The efficiency of the integrated treatment system to treat carwash wastewater and to meet Environmental Quality Act (EQA 1974) was evaluated based on the analysis of pH, dissolved oxygen (DO), chemical oxygen demand (COD) and turbidity (NTU). The integrated treatment system was efficient for treatment of raw carwash wastewater. The treated carwash wastewaters meet EQA 1974 regulation 2009 (Standards A) in the term of pH and DO while, turbidity and COD reduced in the wastewater to meet Standards B. The integrated treatment system designed here with natural coagulant (M. oleifera) and filtration unit were effective for primary treatment of carwash wastewater before the final disposal or to be reused again for carwash process.

  15. Technical note: Assessment of milk temperature measured by automatic milking systems as an indicator of body temperature and fever in dairy cows.

    PubMed

    Pohl, A; Heuwieser, W; Burfeind, O

    2014-07-01

    The objective of this study was to evaluate whether milk temperature (MT) measured by automatic milking system (AMS) is a reliable indicator of body temperature of dairy cows and whether cows with fever could be detected. Data loggers (Minilog 8, Vemco Ltd., Halifax, NS, Canada) measuring body temperature were inserted for 7 ± 1 d into the vaginal cavity of 31 dairy cows and programmed to take 1 reading/min. Milk temperature was recorded at each milking event by the AMS, and values from the vaginal loggers were paired with the corresponding MT. The correlation (r) between vaginal temperature (VT) and MT was 0.52. Vaginal temperature was higher (39.1 ± 0.4°C) than MT (38.6 ± 0.7°C) with a mean difference of 0.5 ± 0.6°C. The ability of MT to identify cows with fever was assessed using 2 approaches. In the first approach, VT could indicate fever at any time of the day, whereas MT could display fever only during the milking events of a given day. Different definitions of fever based on thresholds of VT and duration exceeding these thresholds were constructed. Different thresholds of MT were tested to distinguish between cows with and without fever. The combination of 39.0°C as a threshold for MT and 39.5°C for at least 2h/d as a threshold for VT resulted in the highest combination of sensitivity (0.65) and specificity (0.65). In the second approach, we evaluated whether MT could identify cows with fever at a given milking event. A threshold of MT >38.7°C delivered the best combination of sensitivity (0.77) and specificity (0.66) when fever was defined as VT ≥39.5°C. Therefore, MT measured by AMS can be indicative of fever in dairy cows to a limited extent. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  16. Herd factors associated with dairy cow mortality.

    PubMed

    McConnel, C; Lombard, J; Wagner, B; Kopral, C; Garry, F

    2015-08-01

    Summary studies of dairy cow removal indicate increasing levels of mortality over the past several decades. This poses a serious problem for the US dairy industry. The objective of this project was to evaluate associations between facilities, herd management practices, disease occurrence and death rates on US dairy operations through an analysis of the National Animal Health Monitoring System's Dairy 2007 survey. The survey included farms in 17 states that represented 79.5% of US dairy operations and 82.5% of the US dairy cow population. During the first phase of the study operations were randomly selected from a sampling list maintained by the National Agricultural Statistics Service. Only farms that participated in phase I and had 30 or more dairy cows were eligible to participate in phase II. In total, 459 farms had complete data for all selected variables and were included in this analysis. Univariable associations between dairy cow mortality and 162 a priori identified operation-level management practices or characteristics were evaluated. Sixty of the 162 management factors explored in the univariate analysis met initial screening criteria and were further evaluated in a multivariable model exploring more complex relationships. The final weighted, negative binomial regression model included six variables. Based on the incidence rate ratio, this model predicted 32.0% less mortality for operations that vaccinated heifers for at least one of the following: bovine viral diarrhea, infectious bovine rhinotracheitis, parainfluenza 3, bovine respiratory syncytial virus, Haemophilus somnus, leptospirosis, Salmonella, Escherichia coli or clostridia. The final multivariable model also predicted a 27.0% increase in mortality for operations from which a bulk tank milk sample tested ELISA positive for bovine leukosis virus. Additionally, an 18.0% higher mortality was predicted for operations that used necropsies to determine the cause of death for some proportion of dead

  17. Coxiella burnetii shedding by dairy cows.

    PubMed

    Guatteo, Raphaël; Beaudeau, François; Joly, Alain; Seegers, Henri

    2007-01-01

    While shedding routes of Coxiella burnetii are identified, the characteristics of Coxiella shedding are still widely unknown, especially in dairy cattle. However, this information is crucial to assess the natural course of Coxiella burnetii infection within a herd and then to elaborate strategies to limit the risks of transmission between animals and to humans. The present study aimed at (i) describing the characteristics of Coxiella burnetii shedding by dairy cows (in milk, vaginal mucus, faeces) in five infected dairy herds, and at (ii) investigating the possible relationships between shedding patterns and serological responses. A total of 145 cows were included in a follow-up consisting of seven concomitant samplings of milk, vaginal mucus, faeces and blood (Day 0, D7, D14, D21, D28, D63, D90). Detection and quantification of Coxiella burnetii titres were performed in milk, vaginal mucus and faeces samples using real-time PCR assay, while antibodies against Coxiella were detected using an ELISA technique. For a given shedding route, and a given periodicity (weekly or monthly), cows were gathered into different shedding kinetic patterns according to the sequence of PCR responses. Distribution of estimated titres in Coxiella burnetii was described according to shedding kinetic patterns. Coxiella burnetii shedding was found scarcely and sporadically in faeces. Vaginal mucus shedding concerned almost 50% of the cows studied and was found intermittently or sporadically, depending on the periodicity considered. Almost 40% of cows were detected as milk shedders, with two predominant shedding patterns: persistent and sporadic, regardless of the sampling periodicity. Significantly higher estimated titres in Coxiella burnetii were observed in cows with persistent shedding patterns suggesting the existence of heavy shedder cows. These latter cows were mostly, persistently highly-seropositive, suggesting that repeated serological testings could be a reliable tool to screen

  18. A Case Study of Behaviour and Performance of Confined or Pastured Cows During the Dry Period.

    PubMed

    Black, Randi A; Krawczel, Peter D

    2016-07-13

    The objectives of this study were to determine the effect of the dry cow management system (pasture or confined) on: (1) lying behaviour and activity; (2) feeding and heat stress behaviours; (3) intramammary infections, postpartum. Non-lactating Holstein cows were assigned to either deep-bedded, sand freestalls ( n = 14) or pasture ( n = 14) using rolling enrollment. At dry-off, cows were equipped with an accelerometer to determine daily lying time (h/d), lying bouts (bouts/d), steps (steps/d) and divided into periods: far-off (60 to 15 d prepartum), close-up (14 to 1 d prepartum), calving (calving date) and postpartum (1 to 14 d postpartum). Respiration rates were recorded once weekly from dry off to calving from 1300 to 1500 h. Feeding displacements were defined as one cow successfully displacing another from the feed bunk and were recorded once per week during the 2 h period, immediately after feeding at 800 h. Pastured cows were fed a commercial dry cow pellet during far-off and total mixed ration during close-up, with free access to hay and grazing. Freestall housed cows were fed a total mixed ration at far-off and close-up. Cows housed in freestalls were moved to a maternity pen with a mattress at commencement of labour. Pastured cows calved in pasture. After calving, all cows were commingled in a pen identical to the freestall housing treatment. Cows housed in freestalls laid down for longer during far-off and close-up periods, had fewer lying bouts during the calving period and took fewer steps throughout the study period when compared to pastured cows. Freestall housed cows experienced more displacements after feeding than did pastured cows. Respiration rates increased with an increasing temperature humidity index, more in pastured cows than in freestall housed cows. Pastured cows altered their lying behaviour and activity, suggesting a shift in time budget priorities between pastured and confined dry cows. Pastured cows also experienced less aggression

  19. Lameness Detection in Dairy Cows: Part 2. Use of Sensors to Automatically Register Changes in Locomotion or Behavior.

    PubMed

    Van Nuffel, Annelies; Zwertvaegher, Ingrid; Van Weyenberg, Stephanie; Pastell, Matti; Thorup, Vivi M; Bahr, Claudia; Sonck, Bart; Saeys, Wouter

    2015-08-28

    Despite the research on opportunities to automatically measure lameness in cattle, lameness detection systems are not widely available commercially and are only used on a few dairy farms. However, farmers need to be aware of the lame cows in their herds in order treat them properly and in a timely fashion. Many papers have focused on the automated measurement of gait or behavioral cow characteristics related to lameness. In order for such automated measurements to be used in a detection system, algorithms to distinguish between non-lame and mildly or severely lame cows need to be developed and validated. Few studies have reached this latter stage of the development process. Also, comparison between the different approaches is impeded by the wide range of practical settings used to measure the gait or behavioral characteristic (e.g., measurements during normal farming routine or during experiments; cows guided or walking at their own speed) and by the different definitions of lame cows. In the majority of the publications, mildly lame cows are included in the non-lame cow group, which limits the possibility of also detecting early lameness cases. In this review, studies that used sensor technology to measure changes in gait or behavior of cows related to lameness are discussed together with practical considerations when conducting lameness research. In addition, other prerequisites for any lameness detection system on farms (e.g., need for early detection, real-time measurements) are discussed.

  20. Effectiveness of Commercial and Homemade Washing Agents in Removing Pesticide Residues on and in Apples.

    PubMed

    Yang, Tianxi; Doherty, Jeffery; Zhao, Bin; Kinchla, Amanda J; Clark, John M; He, Lili

    2017-11-08

    Removal of pesticide residues from fresh produce is important to reduce pesticide exposure to humans. This study investigated the effectiveness of commercial and homemade washing agents in the removal of surface and internalized pesticide residues from apples. Surface-enhanced Raman scattering (SERS) mapping and liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were used to determine the effectiveness of different washing agents in removing pesticide residues. Surface pesticide residues were most effectively removed by sodium bicarbonate (baking soda, NaHCO 3 ) solution when compared to either tap water or Clorox bleach. Using a 10 mg/mL NaHCO 3 washing solution, it took 12 and 15 min to completely remove thiabendazole or phosmet surface residues, respectively, following a 24 h exposure to these pesticides, which were applied at a concentration of 125 ng/cm 2 . LC-MS/MS results showed, however, that 20% of applied thiabendazole and 4.4% of applied phosmet had penetrated into the apples following the 24 h exposure. Thiabendazole, a systemic pesticide, penetrated 4-fold deeper into the apple peel than did phosmet, a non-systemic pesticide, which led to more thiabendazole residues inside the apples, which could not be washed away using the NaHCO 3 washing solution. This study gives us the information that the standard postharvest washing method using Clorox bleach solution for 2 min is not an effective means to completely remove pesticide residues on the surface of apples. The NaHCO 3 method is more effective in removing surface pesticide residues on apples. In the presence of NaHCO 3 , thiabendazole and phosmet can degrade, which assists the physical removal force of washing. However, the NaHCO 3 method was not completely effective in removing residues that have penetrated into the apple peel. The overall effectiveness of the method to remove all pesticide residues diminished as pesticides penetrated deeper into the fruit. In practical application

  1. 30 CFR 206.259 - Determination of washing allowances.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) Allowable maintenance expenses include: Maintenance of the wash plant; maintenance of equipment; maintenance..., or a cost equal to the depreciable investment in the wash plant multiplied by the rate of return in... depreciable fixed assets (including costs of delivery and installation of capital equipment) which are an...

  2. The influence of the environment on dairy cow behavior, claw health and herd lameness dynamics.

    PubMed

    Cook, Nigel B; Nordlund, Kenneth V

    2009-03-01

    Free stall housing increases the exposure of dairy cows' claws to concrete walk-ways and to manure between periods of rest, and generally shows the highest rate of lameness compared with other dairy management systems. However, there is great variation within a system, and the rate of new cases of lameness can be reduced to very low levels provided time spent resting per day is maximized through good stall design, access to stalls through stocking density control and comfortable transition cow facilities, limiting the time spent milking, provision of adequate heat abatement, and good leg hygiene. Sand bedded stalls are useful as they also permit lame cows to maintain adequate daily rest. Rubberized alley flooring surfaces benefit the cow by reducing claw wear and trauma compared to concrete, making them ideal for parlor holding areas and long transfer lanes and walk ways. However, caution is required when using rubber floors in pens with uncomfortable stalls due to apparent adverse effects on cow time budgets, which may in turn have a detrimental effect on lameness.

  3. Is downer cow syndrome related to chronic botulism?

    PubMed

    Rulff, R; Schrödl, W; Basiouni, S; Neuhaus, J; Krüger, M

    2015-01-01

    The present work was directed to investigate the relationship between Downer cow syndrome (DCS) and chronic botulism in dairy cattle. For this purpose, a total of 52 fresh calving downer cows and 206 apparently healthy cows at 14 dairy farms were investigated for Clostridium botulinum ABE and CD antibody levels, C. botulinum and botulinum neurotoxin in rumen fluids as well as in faeces. Results indicated that the downer cows had higher IgG titers for C. botulinum ABE and CD than the healthy cows. All tested rumen fluids were negative for BoNT and C. botulinum. BoNT/D, however, and C. botulinum type D spores were detected in faecal samples of healthy and downer cows in the selected farms. In conclusion, the presence of a significantly higher C. botulinum ABE and CD antibody levels in DCS cows than in the healthy animals suggests that chronic C. botulinum toxico-infection could be a predisposing factor for DCS.

  4. Economic costs of recorded reasons for cow mortality and culling in a pasture-based dairy industry.

    PubMed

    Kerslake, J I; Amer, P R; O'Neill, P L; Wong, S L; Roche, J R; Phyn, C V C

    2018-02-01

    The objective of this study was to determine the economic costs associated with different reasons for cow culling or on-farm mortality in a pasture-based seasonal system. A bioeconomic model was developed to quantify costs associated with the different farmer-recorded reasons and timing of cow wastage. The model accounted for the parity and stage of lactation at which the cows were removed as well as the consequent effect on the replacement rate and average age structure of the herd. The costs and benefits associated with the change were quantified, including animal replacement cost, cull salvage value, milk production loss, and the profitability of altered genetic merit based on industry genetic trends for each parity. The total cost of cow wastage was estimated to be NZ$23,628/100 cows per year (NZ$1 = US$0.69) in a pasture-based system. Of this total cost, NZ$14,300/100 cows worth of removals were for nonpregnancy and unknown reasons, and another NZ$3,631/100 cows was attributed to low milk production, mastitis, and udder problems. The total cost for cow removals due to farmer-recorded biological reasons (excluding unknown, production, and management-related causes) was estimated to be NZ$13,632/100 cows per year. Of this cost, an estimated NZ$10,286/100 cows was attributed to nonpregnancy, mastitis, udder problems, calving trouble, and injury or accident. There is a strong economic case for the pasture-based dairy industries to invest in genetic, herd health, and production management research focused on reducing animal wastage due to reproductive failure, mastitis, udder problems, injuries or accidents, and calving difficulties. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  5. BLAISDELL SLOW SAND FILTER WASHING MACHINE. VIEW LOOKING SOUTHWEST. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BLAISDELL SLOW SAND FILTER WASHING MACHINE. VIEW LOOKING SOUTHWEST. - Yuma Main Street Water Treatment Plant, Blaisdell Slow Sand Filter Washing Machine, Jones Street at foot of Main Street, Yuma, Yuma County, AZ

  6. Randomized study of washing 40- to 42-day-stored red blood cells.

    PubMed

    Bennett-Guerrero, Elliott; Kirby, Brett S; Zhu, Hongmei; Herman, Annadele E; Bandarenko, Nicholas; McMahon, Timothy J

    2014-10-01

    Pretransfusion washing of red blood cells (RBCs) stored for a longer duration may have theoretical advantages but few data exist to support this practice. In many hospital settings, use of a point-of-care cell washer could conceivably be used to quickly wash allogeneic RBCs before transfusion. The purpose of this preliminary study was to compare a point-of-care device with a common blood bank device for washing longer-stored RBCs. Forty RBC units stored for 40 to 42 days were randomized to washing with the COBE 2991 device (Terumo BCT; FDA-cleared for washing stored RBCs) or the Cell Saver Elite (Haemonetics; FDA-cleared point-of-care device for processing and washing fresh autologous shed whole blood). Supernatant and unit RBCs from unwashed (baseline) and washed blood were assayed for potassium, lactate, intracellular ATP, percentage of RBC recovery, cell-free hemoglobin, RBC microparticles, and RBCs were examined for susceptibility to hemolysis by physical stress. Both devices recovered a high percentage of RBCs and efficiently removed extracelluar potassium. Washing with the Elite resulted in significant increases in cell-free Hb, percent hemolysis, and RBC microparticle production, whereas washing with the COBE 2991 did not (fold Δ = 2.1 vs. 1.0, 4.6 vs. 1.2, 2.0 vs. 1.1, respectively; p < 0.05). Hemolysis induced by physical stress was not altered by washing. Although point-of-care washing of longer-stored RBCs is appealing, these preliminary data suggest that transfusion of washed, longer-stored units could result in potentially greater exposure to plasma free Hb. More data are needed before this practice can be routinely recommended. © 2014 AABB.

  7. 33 CFR 110.230 - Puget Sound Area, Wash.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Puget Sound Area, Wash. 110.230... ANCHORAGE REGULATIONS Anchorage Grounds § 110.230 Puget Sound Area, Wash. (a) The anchorage grounds—(1... shores of Whidbey Island. (4) Port Gardner General Anchorage, Possession Sound. Beginning at a point...

  8. 33 CFR 110.230 - Puget Sound Area, Wash.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Puget Sound Area, Wash. 110.230... ANCHORAGE REGULATIONS Anchorage Grounds § 110.230 Puget Sound Area, Wash. (a) The anchorage grounds—(1... shores of Whidbey Island. (4) Port Gardner General Anchorage, Possession Sound. Beginning at a point...

  9. Comparison of tenderness, palatability, and retail caselife of enhanced cow subprimals with nonenhanced cow and United States Department of Agriculture Select subprimals.

    PubMed

    Rose, M N; Garmyn, A J; Hilton, G G; Morgan, J B; VanOverbeke, D L

    2010-11-01

    The objective of this study was to determine the effects of enhancing cow subprimals on tenderness, palatability, and retail caselife compared with nonenhanced cow and USDA Select subprimals. Strip loin, top sirloin butt, and ribeye subprimals were selected (n = 60 per subprimal; n = 40 cow, n = 20 USDA Select). At 14 d postmortem, one-half of the cow product was randomly selected for enhancement with phosphate, salt, rosemary, potassium lactate, beef stock, ice, and water using a needle injector. All subprimals were fabricated on d 14 postmortem into ten 2.54-cm steaks. Steaks were assigned to analysis for Warner-Bratzler shear force (WBSF), trained sensory panel evaluation, or simulated retail display and assigned randomly to aging periods of 14, 21, or 28 d postmortem. Warner-Bratzler shear force values of ribeye steaks from the nonenhanced cow over all aging groups were significantly greater (P < 0.05) than enhanced cow and USDA Select ribeyes. Furthermore, top sirloin butts from nonenhanced cow had greater (P < 0.05) WBSF values at 14 and 28 d compared with other treatments. Nonenhanced cow strip loin steaks had greater (P < 0.05) WBSF values (4.58 ± 0.15 kg) and greater (P < 0.05) cook loss (20.70 ± 0.68 kg) than enhanced cow (3.90 ± 0.14 kg; 14.32 ± 0.69 kg) and USDA Select steaks (3.09 ± 0.14 kg; 15.01 ± 0.68 kg). Sensory data revealed that enhanced cow steaks from all subprimals were greater (P < 0.05) for initial juiciness for both aging periods, compared with nonenhanced cow and USDA Select. Sensory tenderness ratings for enhanced cow and USDA Select subprimals were greater (P < 0.05) than nonenhanced subprimals. Nonenhanced subprimals received the largest connective tissue amount score by panelists (P < 0.05). Enhanced cow steaks from all 3 subprimals had a greater intensity (P < 0.05) for salty flavor. Panelists detected a more intense (P < 0.05) soapy flavor in enhanced ribeyes. In ribeye and top sirloin butt steaks, grassy/cowy flavor was more

  10. Technical note: Data loggers are a valid method for assessing the feeding behavior of dairy cows using the Calan Broadbent Feeding System.

    PubMed

    Krawczel, P D; Klaiber, L M; Thibeau, S S; Dann, H M

    2012-08-01

    Assessing feeding behavior is important in understanding the effects of nutrition and management on the well-being of dairy cows. Historically, collection of these data from cows fed with a Calan Broadbent Feeding System (American Calan Inc., Northwood, NH) required the labor-intensive practices of direct observation or video review. The objective of this study was to evaluate the agreement between the output of a HOBO change-of-state data logger (Onset Computer Corp., Bourne, MA), mounted to the door shell and latch plate, and video data summarized with continuous sampling. Data (number of feed bin visits per day and feeding time in minutes per day) were recorded with both methods from 26 lactating cows and 10 nonlactating cows for 3 d per cow (n=108). The agreement of the data logger and video methods was evaluated using the REG procedure of SAS to compare the mean response of the methods against the difference between the methods. The maximum allowable difference (MAD) was set at ±3 for bin visits and ±20 min for feeding time. Ranges for feed bin visits (2 to 140 per d) and feeding time (28 to 267 min/d) were established from video data. Using the complete data set, agreement was partially established between the data logger and video methods for feed bin visits, but not established for feeding time. The complete data set generated by the data logger was screened to remove visits of a duration ≤3 s, reflecting a cow unable to enter a feed bin (representing 7% of all data) and ≥5,400 s, reflecting a failure of the data logger to align properly with its corresponding magnetic field (representing <1% of all data). Using the resulting screened data set, agreement was established for feed bin visits and feeding time. For bin visits, 4% of the data was beyond the MAD. For feeding time, 3% of the data was beyond the MAD and 74% of the data was ±1 min. The insignificant P-value, low coefficient of determination, and concentration of the data within the MAD

  11. Supplementation of organic and inorganic selenium to late gestation and early lactation beef cows effect on cow and preweaning calf performance.

    PubMed

    Muegge, C R; Brennan, K M; Schoonmaker, J P

    2016-08-01

    Angus × Simmental cows ( = 48; BW = 595 ± 17.4 kg, BCS = 5.26 ± 0.05, and age = 2.3 ± 0.07 yr), pregnant with male fetuses, were used to determine the effect of Se source during the last 80 d of gestation and first 108 d of lactation on cow and calf performance. At 203 d in gestation, cows were blocked by BW, breed composition, age, and calf sire and randomly allotted to organic Se, inorganic Se, or no Se treatments. Diets contained corn silage, corn stover, haylage, dried distillers' grains with solubles, and minerals and were formulated to contain 10.4% CP and 0.90 Mcal/kg NEg during gestation and 12.1% CP and 1.01 Mcal/kg NEg during lactation. Diets were fed daily as a total mixed ration and none, 3 mg/d Se as sodium selenite, or 3 mg/d Se as Sel-Plex were top-dressed daily. At 68 d postpartum (DPP), milk production was calculated using the weigh-suckle-weigh procedure and a milk sample was collected to determine composition. At 108 DPP, cow-calf pairs were commingled until weaning at 210 DPP. Cow BW and BCS ( ≥ 0.56) did not differ between treatments at any time point during the study. Milk production, milk fat, and total solids ( ≥ 0.38) did not differ among treatments. Milk protein tended to increase in cows fed inorganic Se compared with cows fed organic Se ( = 0.07) and milk lactose tended to be greatest in cows fed organic Se ( = 0.10). Conception to AI and overall pregnancy rates did not differ between treatments ( ≥ 0.39). Calf weights and ADG did not differ through 108 DPP ( ≥ 0.77) or for the preweaning period ( ≥ 0.33). Plasma Se concentration was adequate for all cows and did not differ among treatments for cows ( ≥ 0.37) or calves ( ≥ 0.90). Liver Se concentrations in cows fed inorganic or organic Se were greater than in control cows ( < 0.01). Longissimus muscles biopsies taken from progeny at 108 DPP also did not differ between treatments ( = 0.45). In conclusion, dietary Se source did not affect cow performance, milk production

  12. Removal of heavy metals from polluted soil using the citric acid fermentation broth: a promising washing agent.

    PubMed

    Zhang, Hongjiao; Gao, Yuntao; Xiong, Huabin

    2017-04-01

    The citric acid fermentation broth was prepared and it was employed to washing remediation of heavy metal-polluted soil. A well-defined washing effect was obtained, the removal percentages using citric acid fermentation broth are that 48.2% for Pb, 30.6% for Cu, 43.7% for Cr, and 58.4% for Cd and higher than that using citric acid solution. The kinetics of heavy metals desorption can be described by the double constant equation and Elovich equation and is a heterogeneous diffusion process. The speciation analysis shows that the citric acid fermentation broth can effectively reduce bioavailability and environmental risk of heavy metals. Spectroscopy characteristics analysis suggests that the washing method has only a small effect on the mineral composition and does not destroy the framework of soil system. Therefore, the citric acid fermentation broth is a promising washing agent and possesses a potential practical application value in the field of remediation of soils with a good washing performance.

  13. 33 CFR 162.235 - Puget Sound Area, Wash.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Puget Sound Area, Wash. 162.235...) PORTS AND WATERWAYS SAFETY INLAND WATERWAYS NAVIGATION REGULATIONS § 162.235 Puget Sound Area, Wash. (a.... Southbound boats shall sound the signal within 600 yards of Port Townsend Canal Light. Northbound boats shall...

  14. Diversity of Staphylococcus species and prevalence of enterotoxin genes isolated from milk of healthy cows and cows with subclinical mastitis.

    PubMed

    Rall, V L M; Miranda, E S; Castilho, I G; Camargo, C H; Langoni, H; Guimarães, F F; Araújo Júnior, J P; Fernandes Júnior, A

    2014-02-01

    The objectives of this study were to determine the occurrence and diversity of Staphylococcus spp. in milk from healthy cows and cows with subclinical mastitis in Brazil and to examine the profile of enterotoxin genes and some enterotoxins produced by Staphylococcus spp. A total of 280 individual mammary quarter milk samples from 70 healthy cows and 292 samples from 73 cows with subclinical mastitis were collected from 11 farms in the state of São Paulo, Brazil. Staphylococcus spp. were recovered from 63 (22.5%) samples from healthy cows and from 80 samples (27.4%) from cows with mastitis. The presence of Staphylococcus aureus was significantly different between these 2 groups and was more prevalent in the cows with mastitis. The presence of Staphylococcus saprophyticus was also significantly different between these 2 groups, but this organism was more prevalent in healthy cows. No statistically significant differences were observed in the numbers of other staphylococci in milk samples from the 2 groups. The sea gene was the most prevalent enterotoxin gene in both groups. Eight of 15 (53.3%) Staph. aureus carried this gene and all produced the SEA toxin. In the coagulase-negative staphylococci (CNS) group, 61 of 128 (47.5%) had the same gene and just 1 (1.6%) Staphylococcus epidermidis strain produced the enterotoxin in vitro. Because CNS were isolated from both groups of cows and most CNS contained enterotoxin genes but did not produce toxins, the role of CNS in mastitis should be carefully defined. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  15. Hand washing with soap and WASH educational intervention reduces under-five childhood diarrhoea incidence in Jigjiga District, Eastern Ethiopia: A community-based cluster randomized controlled trial.

    PubMed

    Hashi, Abdiwahab; Kumie, Abera; Gasana, Janvier

    2017-06-01

    Despite the tremendous achievement in reducing child mortality and morbidity in the last two decades, diarrhoea is still a major cause of morbidity and mortality among children in many developing countries, including Ethiopia. Hand washing with soap promotion, water quality improvements and improvements in excreta disposal significantly reduces diarrhoeal diseases. The objective of this study was to evaluate the effect of hand washing with soap and water, sanitation and hygiene (WASH) educational Intervention on the incidence of under-five children diarrhoea. A community-based cluster randomized controlled trial was conducted in 24 clusters (sub-Kebelles) in Jigjiga district, Somali region, Eastern Ethiopia from February 1 to July 30, 2015. The trial compared incidence of diarrhoea among under-five children whose primary caretakers receive hand washing with soap and water, sanitation, hygiene educational messages with control households. Generalized estimating equation with a log link function Poisson distribution family was used to compute adjusted incidence rate ratio and the corresponding 95% confidence interval. The results of this study show that the longitudinal adjusted incidence rate ratio (IRR) of diarrhoeal diseases comparing interventional and control households was 0.65 (95% CI 0.57, 0.73) suggesting an overall diarrhoeal diseases reduction of 35%. The results are similar to other trials of WASH educational interventions and hand washing with soap. In conclusion, hand washing with soap practice during critical times and WASH educational messages reduces childhood diarrhoea in the rural pastoralist area.

  16. Detection of relevant amounts of cow's milk protein in non-pre-packed bakery products sold as cow's milk-free.

    PubMed

    Trendelenburg, V; Enzian, N; Bellach, J; Schnadt, S; Niggemann, B; Beyer, K

    2015-05-01

    Currently, there is no mandatory labelling of allergens for non-pre-packed foods in the EU. Therefore, consumers with food allergy rely on voluntary information provided by the staff. The aim of this study was to characterize allergic reactions to non-pre-packed foods and to investigate whether staff in bakery shops were able to give advice regarding a safe product choice. Questionnaires were sent to 200 parents of children with a food allergy. Staff of 50 bakery shops were interviewed regarding selling non-pre-packed foods to food-allergic customers. Bakery products being recommended as 'cow's milk-free' were bought, and cow's milk protein levels were measured using ELISA. A total of 104 of 200 questionnaires were returned. 25% of the children experienced an allergic reaction due to a non-pre-packed food from bakery shops and 20% from ice cream parlours. Sixty percent of the bakery staff reported serving food-allergic customers at least once a month, 24% once a week. Eighty four percent of the staff felt able to advise food-allergic consumers regarding a safe product choice. Seventy three 'cow's milk-free' products were sold in 44 bakery shops. Cow's milk could be detected in 43% of the bakery products, 21% contained >3 mg cow's milk protein per serving. Staff in bakery shops felt confident about advising customers with food allergy. However, cow's milk was detectable in almost half of bakery products being sold as 'cow's milk-free'. Every fifth product contained quantities of cow's milk exceeding an amount where approximately 10% of cow's milk-allergic children will show clinical relevant symptoms. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  17. WASH and gender in health care facilities: The uncharted territory.

    PubMed

    Kohler, Petra; Renggli, Samuel; Lüthi, Christoph

    2017-11-08

    Health care facilities in low- and middle-income countries are high-risk settings, and face special challenges to achieving sustainable water, sanitation, and hygiene (WASH) services. Our applied interdisciplinary research conducted in India and Uganda analyzed six dimensions of WASH services in selected health care facilities, including menstrual hygiene management. To be effective, WASH monitoring strategies in health care facilities must include gender sensitive measures. We present a novel strategy, showing that applied gender sensitive multitool assessments are highly productive in assessments of WASH services and facilities from user and provider perspectives. We discuss its potential for applications at scale and as an area of future research.

  18. Influence of the washing program on the blood processing performance of a continuous autotransfusion device.

    PubMed

    Yoon, Chiyul; Noh, Seungwoo; Lee, Jung Chan; Ko, Sung Ho; Ahn, Wonsik; Kim, Hee Chan

    2014-03-01

    The continuous autotransfusion system has been widely used in surgical operations. It is known that if oil is added to blood, and this mixture is then processed by an autotransfusion device, the added oil is removed and reinfusion of fat is prevented by the device. However, there is no detailed report on the influence of the particular washing program selected on the levels of blood components including blood fat after continuous autotransfusion using such a system. Fresh bovine blood samples were processed by a commercial continuous autotransfusion device using the "emergency," "quality," and "high-quality" programs, applied in random order. Complete blood count (CBC) and serum chemistry were analyzed to determine how the blood processing performance of the device changes with the washing program applied. There was no significant difference in the CBC results obtained with the three washing programs. Although all of the blood lipids in the processed blood were decreased compared to those in the blood before processing, the levels of triglyceride, phospholipid, and total cholesterol after processing via the emergency program were significantly higher than those present after processing via the quality and high-quality programs. Although the continuous autotransfusion device provided consistent hematocrit quality, the levels of some blood lipid components showed significant differences among the washing programs.

  19. Challenges and Opportunities for Mainstreaming Climate Change Adaptation into WaSH Development Planning in Ghana.

    PubMed

    Alhassan, Salley; Hadwen, Wade L

    2017-07-10

    Climate change threatens water, sanitation and hygiene (WaSH) facilities and services, as these are intimately linked to the water cycle and are vulnerable to changes in the quantity and quality of available water resources. Floods and droughts, which pollute and reduce water delivery respectively, have now become a perennial issue to deal with in the northern regions of Ghana. This study aimed to assess the degree to which climate change adaptation measures are mainstreamed into the water, sanitation and hygiene (WaSH) development planning process in Ghana. Stakeholders from government and non-government agencies were interviewed to gain perspectives on the threat of climate change, the inclusion of climate change in WaSH planning and the barriers preventing mainstreaming. Despite awareness of climate change, adaptation measures have not been considered, and the immediate WaSH needs remain the priority. Overall, stakeholders felt the adaptive capacity of the Municipality was low and that mainstreaming has not yet occurred. Despite the lack of progress, there are great opportunities for mainstreaming climate change adaptation into planning through increasing awareness and capacity, legislative and institutional changes and the development of participatory systems to provide early warning systems and disaster risk analyses that will inform future planning.

  20. Challenges and Opportunities for Mainstreaming Climate Change Adaptation into WaSH Development Planning in Ghana

    PubMed Central

    2017-01-01

    Climate change threatens water, sanitation and hygiene (WaSH) facilities and services, as these are intimately linked to the water cycle and are vulnerable to changes in the quantity and quality of available water resources. Floods and droughts, which pollute and reduce water delivery respectively, have now become a perennial issue to deal with in the northern regions of Ghana. This study aimed to assess the degree to which climate change adaptation measures are mainstreamed into the water, sanitation and hygiene (WaSH) development planning process in Ghana. Stakeholders from government and non-government agencies were interviewed to gain perspectives on the threat of climate change, the inclusion of climate change in WaSH planning and the barriers preventing mainstreaming. Despite awareness of climate change, adaptation measures have not been considered, and the immediate WaSH needs remain the priority. Overall, stakeholders felt the adaptive capacity of the Municipality was low and that mainstreaming has not yet occurred. Despite the lack of progress, there are great opportunities for mainstreaming climate change adaptation into planning through increasing awareness and capacity, legislative and institutional changes and the development of participatory systems to provide early warning systems and disaster risk analyses that will inform future planning. PMID:28698518

  1. Frequency of Neospora caninum infections in beef cow-calf operations under extensive management.

    PubMed

    Rodríguez, A M; Maresca, S; Cano, D B; Armendano, J I; Combessies, G; Lopéz-Valiente, S; Odriozola, E R; Späth, E J L; Odeón, A C; Campero, C M; Moore, D P

    2016-03-30

    The aim of this study was to evaluate the frequencies of Neospora caninum horizontal and vertical transmissions in beef cow-calf operations under three different extensive management systems: group A: 0.75 head per hectare pasturing on natural grass; group B: 1.1 head per hectare on natural grass and improved cultured pastures; and group C: 2 head per hectare on natural grass, improved cultured pasture and whole corn silage. Serum samples from 72 multiparous cows assigned to each beef cow-calf operations were obtained every 3 months during 2 years. A group of 30 replacement heifers from each group were tested similarly since they were 10-21 months old. Twenty four, 20 and 34 calves from groups A, B and C respectively, were bled before colostrum intake and again 6 months later. The samples were analyzed by indirect fluorescence antibody test (IFAT) for detection of total IgG against N. caninum at a serological titre ≥ 200 for multiparous cows and replacement heifers, and a serological titre ≥ 25 for calves. Serum samples from seropositive cows were assessed by ELISA to evaluate the avidity of their specific antibodies. There were no differences in the proportion of seropositive cows from groups A, B and C at the beginning of the trial (p>0.05). Interestingly, the lowest serological titres in seropositive cows from all groups were observed during the first trimester (p<0.05). Although seropositive cows had medium to high avidity antibodies, suggesting chronic infection; seroconversion associated with low antibody avidity was found in 2, 3 and 3 seropositive cows from groups A, B and C. All replacement heifers remained seronegative. No abortions were recorded but 2, 1, and 2 calves from groups A, B and C were seropositive before colostrum intake, respectively. Seropositive calves born from cows having intermediate or high avidity remained with the same serostatus at 6 months of age. Even under varying extensive management conditions, both N. caninum horizontal and

  2. The suitability of EIT to estimate EELV in a clinical trial compared to oxygen wash-in/wash-out technique.

    PubMed

    Karsten, Jan; Meier, Torsten; Iblher, Peter; Schindler, Angela; Paarmann, Hauke; Heinze, Hermann

    2014-02-01

    Open endotracheal suctioning procedure (OSP) and recruitment manoeuvre (RM) are known to induce severe alterations of end-expiratory lung volume (EELV). We hypothesised that EIT lung volumes lack clinical validity. We studied the suitability of EIT to estimate EELV compared to oxygen wash-in/wash-out technique. Fifty-four postoperative cardiac surgery patients were enrolled and received standardized ventilation and OSP. Patients were randomized into two groups receiving either RM after suctioning (group RM) or no RM (group NRM). Measurements were conducted at the following time points: Baseline (T1), after suctioning (T2), after RM or NRM (T3), and 15 and 30 min after T3 (T4 and T5). We measured EELV using the oxygen wash-in/wash-out technique (EELVO2) and computed EELV from EIT (EELVEIT) by the following formula: EELVEITTx,y…=EELVO2+ΔEELI×VT/ΔZ. EELVEIT values were compared with EELVO2 using Bland-Altman analysis and Pearson correlation. Limits of agreement ranged from -0.83 to 1.31 l. Pearson correlation revealed significant results. There was no significant impact of RM or NRM on EELVO2-EELVEIT relationship (p=0.21; p=0.23). During typical routine respiratory manoeuvres like endotracheal suctioning or alveolar recruitment, EELV cannot be estimated by EIT with reasonable accuracy.

  3. Effect of Nitrite/Nitrate concentrations on Corrosivity of Washed Precipitate

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Congdon, J.W.

    2001-03-28

    Cyclic polarization scans were performed using A-537 carbon steel in simulated washed precipitate solutions of various nitrite and nitrate concentrations. The results of this study indicate that nitrate is an aggressive anion in washed precipitate. Furthermore, a quantitative linear log-log relationship between the minimum effective nitrite concentration and the nitrate concentration was established for washed precipitate with other ions at their average compositions.

  4. A Systematic Review: Costing and Financing of Water, Sanitation, and Hygiene (WASH) in Schools

    PubMed Central

    McGinnis, Shannon M.; McKeon, Thomas; Desai, Richa; Ejelonu, Akudo; Laskowski, Stanley; Murphy, Heather M.

    2017-01-01

    Despite the success of recent efforts to increase access to improved water, sanitation, and hygiene (WASH) globally, approximately one-third of schools around the world still lack adequate WASH services. A lack of WASH in schools can lead to the spread of preventable disease and increase school absences, especially among women. Inadequate financing and budgeting has been named as a key barrier for integrating successful and sustainable WASH programs into school settings. For this reason, the purpose of this review is to describe the current knowledge around the costs of WASH components as well as financing models that could be applied to WASH in schools. Results show a lack of information around WASH costing, particularly around software elements as well as a lack of data overall for WASH in school settings as compared to community WASH. This review also identifies several key considerations when designing WASH budgets or selecting financing mechanisms. Findings may be used to advise future WASH in school programs. PMID:28425945

  5. A Systematic Review: Costing and Financing of Water, Sanitation, and Hygiene (WASH) in Schools.

    PubMed

    McGinnis, Shannon M; McKeon, Thomas; Desai, Richa; Ejelonu, Akudo; Laskowski, Stanley; Murphy, Heather M

    2017-04-20

    Despite the success of recent efforts to increase access to improved water, sanitation, and hygiene (WASH) globally, approximately one-third of schools around the world still lack adequate WASH services. A lack of WASH in schools can lead to the spread of preventable disease and increase school absences, especially among women. Inadequate financing and budgeting has been named as a key barrier for integrating successful and sustainable WASH programs into school settings. For this reason, the purpose of this review is to describe the current knowledge around the costs of WASH components as well as financing models that could be applied to WASH in schools. Results show a lack of information around WASH costing, particularly around software elements as well as a lack of data overall for WASH in school settings as compared to community WASH. This review also identifies several key considerations when designing WASH budgets or selecting financing mechanisms. Findings may be used to advise future WASH in school programs.

  6. Washing effects of limonene on pesticide residues in green peppers.

    PubMed

    Lu, Hai-Yan; Shen, Yan; Sun, Xing; Zhu, Hong; Liu, Xian-Jin

    2013-09-01

    The presence of pesticide residues in food has caused much concern. The low health risks and environmental impacts of limonene make it a very interesting solvent for use in green chemistry. Washing effects of limonene on pesticide residues of methyl chlorpyrifos, chlorothalonil, chlorpyrifos, fenpropathrin and deltamethrin were investigated in green pepper. Results showed that washing with a low concentration of limonene for 5 min (where LOQ is limit of quantitation) caused 53.67%, washing with a high concentration were 84.64%, Washing with a low concentration of limonene for 10 min produced 55.90%, washing with a high concentration were 94.42%, washing with tap water (for 10 min) and the emulsion with only egg yolk lecithin (at high concentration for 10 min) were 25.18 %, 37.83%, 21.84%, 20.87%, 13.86% and < LOQ, 59.70%, 54.09%, 54.76%, 54.47%, respectively. The data indicated that washing with a low concentration of limonene for 5 min was the optimal treatment for elimination of pesticide residues in green pepper, considering effect and treatment time as well as cost. © 2013 Society of Chemical Industry.

  7. Droplet-based microfluidic washing module for magnetic particle-based assays

    PubMed Central

    Lee, Hun; Xu, Linfeng; Oh, Kwang W.

    2014-01-01

    In this paper, we propose a continuous flow droplet-based microfluidic platform for magnetic particle-based assays by employing in-droplet washing. The droplet-based washing was implemented by traversing functionalized magnetic particles across a laterally merged droplet from one side (containing sample and reagent) to the other (containing buffer) by an external magnetic field. Consequently, the magnetic particles were extracted to a parallel-synchronized train of washing buffer droplets, and unbound reagents were left in an original train of sample droplets. To realize the droplet-based washing function, the following four procedures were sequentially carried in a droplet-based microfluidic device: parallel synchronization of two trains of droplets by using a ladder-like channel network; lateral electrocoalescence by an electric field; magnetic particle manipulation by a magnetic field; and asymmetrical splitting of merged droplets. For the stable droplet synchronization and electrocoalescence, we optimized droplet generation conditions by varying the flow rate ratio (or droplet size). Image analysis was carried out to determine the fluorescent intensity of reagents before and after the washing step. As a result, the unbound reagents in sample droplets were significantly removed by more than a factor of 25 in the single washing step, while the magnetic particles were successfully extracted into washing buffer droplets. As a proof-of-principle, we demonstrate a magnetic particle-based immunoassay with streptavidin-coated magnetic particles and fluorescently labelled biotin in the proposed continuous flow droplet-based microfluidic platform. PMID:25379098

  8. Clinical ketosis and standing behavior in transition cows.

    PubMed

    Itle, A J; Huzzey, J M; Weary, D M; von Keyserlingk, M A G

    2015-01-01

    Ketosis is a common disease in dairy cattle, especially in the days after calving, and it is often undiagnosed. The objective of this study was to compare the standing behavior of dairy cows with and without ketosis during the days around calving to determine if changes in this behavior could be useful in the early identification of sick cows. Serum β-hydroxybutyrate (BHBA) was measured in 184 cows on a commercial dairy farm twice weekly from 2 to 21d after calving. Standing behavior was measured from 7d before calving to 21d after calving using data loggers. Retrospectively, 15 cows with clinical ketosis (3 consecutive BHBA samples >1.2mmol/L and at least one sample of BHBA >2.9mmol/L) were matched with 15 nonketotic cows (BHBA <1.2mmol/L). Five periods were defined for the statistical analyses: wk -1 (d -7 to -1), d 0 (day of calving), wk +1 (d 1 to 7), wk +2 (d 8 to 14), and wk +3 (d 15 to 21). The first signs of clinical ketosis occurred 4.5±2.1d after calving. Total daily standing time was longer for clinically ketotic cows compared with nonketotic cows during wk -1 (14.3±0.6 vs. 12.0±0.7h/d) and on d 0 (17.2±0.9 vs. 12.7±0.9h/d) but did not differ during the other periods. Clinically ketotic cows exhibited fewer standing bouts compared with nonketotic cows on d 0 only (14.6±1.9 vs. 20.9±1.8bouts/d). Average standing bout duration was also longer for clinically ketotic cows on d 0 compared with nonketotic cows [71.3min/bout (CI: 59.3 to 85.5) vs. 35.8min/bout (CI: 29.8 to 42.9)] but was not different during the other periods. Differences in standing behavior in the week before and on the day of calving may be useful for the early detection of clinical ketosis in dairy cows. Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  9. Lymphocyte functions in dairy cows in hot environment

    NASA Astrophysics Data System (ADS)

    Lacetera, Nicola; Bernabucci, Umberto; Scalia, Daniela; Ronchi, Bruno; Kuzminsky, Giorgina; Nardone, Alessandro

    2005-11-01

    This study was carried out to ascertain the effects of intense high environmental temperatures (HET) on lymphocyte functions in periparturient dairy cows. The study was undertaken from the beginning of March through the end of July 2003 in a commercial dairy unit located approximately 40 km north of Rome. Thirty-four Holstein cows were utilised in the study. Twenty-two of these cows gave birth in spring (SP cows), from 28 March to 30 April. The remaining 12 cows gave birth in summer (SU cows), between 15 June and 2 July. The two groups of cows were balanced for parity and were fed the same rations. Blood samples were taken 4, 3, 2 and 1 week before calving, and 1, 2 and 4 weeks after calving, in order to evaluate peripheral blood mononuclear cell (PBMC) function in vitro, and to determine plasma cortisol concentrations. After isolation, the PBMC were stimulated with mitogens and their response in terms of DNA synthesis and IgM secretion was measured. During spring, either the day (9 20 h) or the night (21 8 h) temperature humidity index (THI) was below the upper critical THI (72) established for dairy cows. During summer, the mean daily THI values were 79.5±2.9 during the day and 70.1±4.7 during the night. Furthermore, during summer, three heat waves (a period of at least 3 consecutive days during which there were less than 10 recovery hours) occurred. Recovery hours were intended hours with a THI below 72. The first heat wave lasted 5 days, the second 6 days, and the third 15 days. Compared to the SP cows, over the entire periparturient period the extent of DNA synthesis and IgM secretion levels were lower (P ranging from <0.01 to 0.0001) and higher (P<0.01) respectively, in the SU cows. Before calving, the SU cows also presented higher (P<0.01) concentrations of plasma cortisol compared to the SP cows. This study indicates that the effects of HET on the immune response depend on the specific immune function under consideration, and that neuroendocrinal changes

  10. 21 CFR 864.9285 - Automated cell-washing centrifuge for immuno-hematology.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Automated cell-washing centrifuge for immuno... Establishments That Manufacture Blood and Blood Products § 864.9285 Automated cell-washing centrifuge for immuno-hematology. (a) Identification. An automated cell-washing centrifuge for immuno-hematology is a device used...

  11. 21 CFR 864.9285 - Automated cell-washing centrifuge for immuno-hematology.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Automated cell-washing centrifuge for immuno... Establishments That Manufacture Blood and Blood Products § 864.9285 Automated cell-washing centrifuge for immuno-hematology. (a) Identification. An automated cell-washing centrifuge for immuno-hematology is a device used...

  12. 21 CFR 864.9285 - Automated cell-washing centrifuge for immuno-hematology.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Automated cell-washing centrifuge for immuno... Establishments That Manufacture Blood and Blood Products § 864.9285 Automated cell-washing centrifuge for immuno-hematology. (a) Identification. An automated cell-washing centrifuge for immuno-hematology is a device used...

  13. 21 CFR 864.9285 - Automated cell-washing centrifuge for immuno-hematology.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Automated cell-washing centrifuge for immuno... Establishments That Manufacture Blood and Blood Products § 864.9285 Automated cell-washing centrifuge for immuno-hematology. (a) Identification. An automated cell-washing centrifuge for immuno-hematology is a device used...

  14. 21 CFR 864.9285 - Automated cell-washing centrifuge for immuno-hematology.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Automated cell-washing centrifuge for immuno... Establishments That Manufacture Blood and Blood Products § 864.9285 Automated cell-washing centrifuge for immuno-hematology. (a) Identification. An automated cell-washing centrifuge for immuno-hematology is a device used...

  15. EPA SITE DEMONSTRATION OF THE BIOTROL SOIL WASHING PROCESS

    EPA Science Inventory

    A pilot-scale soil washing process, patented by BioTrol, Inc., was demonstrate on soil contaminated by wood treating waste, primarily pentachlorophenol (PCP) and creosote-derived polynuclear aromatic hydrocarbons (PAHs). Although soil washing was the main object of this demonstra...

  16. Effects of feed delivery frequency in different environmental conditions on time budget of lactating dairy cows.

    PubMed

    Mattachini, Gabriele; Bava, Luciana; Sandrucci, Anna; Tamburini, Alberto; Riva, Elisabetta; Provolo, Giorgio

    2017-08-01

    This study aimed to examine the influence of feed delivery frequency and environmental conditions on daily time budget of lactating dairy cows. The study was carried out in two commercial dairy farms with Holstein herds. Fifty lactating dairy cows milked in automatic milking units (AMS farm) and 96 primiparous lactating dairy cows milked in a conventional milking parlour (conventional farm) were exposed to different frequencies of feed delivery replicated in different periods of the year (warm and mild) that were characterized by different temperature-humidity indices (THI). On each farm, feeding treatments consisted of two different feed delivery frequencies (1× and 2× on the AMS farm; 2× and 3× on the conventional farm). All behaviours of the cows were monitored for the last 8 d of each treatment period using continuous video recording. The two data sets from different farm systems were considered separately for analysis. On both farms, environmental conditions expressed as THI affected time budgets and the pattern of the behavioural indices throughout the day. The variation in the frequency of feed delivery seems to affect the cow's time budget only in a limited way. Standing time of cows on the conventional farm and the time spent by cows in the milking waiting area on the AMS farm both increased in response to increased feeding frequency. Although feed delivery frequency showed limited influence on cow's time budget, the effect on standing time could be carefully considered, especially on farms equipped with AMS where the type of cow traffic system (e.g., milking first) might amplify the negative consequences of more frequent feed delivery. Further investigations are required to evaluate the effect of THI and feed delivery frequency on other aspects of behavioural activity.

  17. Comparison of cephalonium alone and in combination with an internal teat sealant for dry cow therapy in seasonally calving dairy cows.

    PubMed

    Bates, A J; Chambers, G; Laven, R A

    2016-03-01

    To assess the effect of combining an internal teat sealant (ITS) and a long-acting cephalonium-based dry cow therapy (DCT) on the prevalence of cows with a somatic cell count (SCC) >150,000 cells/mL 60-80 days after calving, and the incidence of clinical mastitis diagnosed by farm staff in the first 100 days after calving. Cows from a spring-calving, pasture-based, dairy farm in the South Canterbury region of New Zealand were randomly allocated to receive cephalonium DCT (n=289) or cephalonium and internal teat sealant (n=304) at the end of lactation. Cows were inspected twice daily by farm staff during the dry period and following calving for signs of mastitis. Individual SCC were determined from herd tests conducted in the previous lactation and following calving. Logistic regression models were used to determine relationships with the prevalence of cows with a SCC >150,000 cells/mL after calving, and survival analysis was used to model time to the first case of clinical mastitis following calving at the cow and quarter level. The OR for a cow with a SCC >150,000 cells/mL after calving, including age and individual SCC in the preceding lactation in the model, was 0.53 (95% CI=0.32-0.89) for cows treated with combination therapy compared to cows receiving cephalonium (p=0.017). At the cow level, including age and preceding SCC in the model, the hazard ratio for diagnosis of clinical mastitis by farm staff in the first 100 days of lactation was 0.60 (95% CI=0.39-0.98) for cows treated with combination therapy compared to cows receiving cephalonium (p=0.04). At the quarter level, the hazard ratio for diagnosis of clinical mastitis, with age included in the model, was 0.41 (95% CI=0.23-0.74) for the combination therapy compared to cephalonium alone (p<0.001). The combination of internal teat sealant and cephalonium DCT was more effective than cephalonium alone at reducing clinical mastitis diagnosed by farm staff in the 100 days after calving, and the prevalence of

  18. Influence of body condition score on live and carcass value of cull beef cows.

    PubMed

    Apple, J K

    1999-10-01

    >.05) to BCS-7 cows. Information from this study can be used by the non-fed beef industry to establish a value-based marketing system. Data from this study would indicate that marketing cull beef cows at a BCS of 6 could optimize economic returns to both cow-calf producers and non-fed beef packers.

  19. Emissions of microplastic fibers from microfiber fleece during domestic washing.

    PubMed

    Pirc, U; Vidmar, M; Mozer, A; Kržan, A

    2016-11-01

    Microplastics are found in marine and freshwater environments; however, their specific sources are not yet well understood. Understanding sources will be of key importance in efforts to reduce emissions into the environment. We examined the emissions of microfibers from domestic washing of a new microfiber polyester fleece textile. Analyzing released fibers collected with a 200 μm filter during 10 mild, successive washing cycles showed that emission initially decreased and then stabilized at approx. 0.0012 wt%. This value is our estimation for the long-term release of fibers during each washing. Use of detergent and softener did not significantly influence emission. Release of fibers during tumble drying was approx. 3.5 times higher than during washing.

  20. [Strengthening Effects of Sodium Salts on Washing Kerosene Contaminated Soil with Surfactants].

    PubMed

    Huang, Zhao-lu; Chen, Quan-yuan; Zhou, Juan; Xie, Mo-han

    2015-05-01

    The impact of sodium salt on kerosene contaminated soil washing with surfactants was investigated. The results indicated that sodium silicate greatly enhanced the washing efficiency of SDS. Sodium tartrate can largely enhance the washing efficiency of SDBS and Brij35. Sodium salts can enhance the washing efficiency on kerosene contaminated with TX-100. No significant differences were observed between different sodium salts. Sodium salt of humic acid and sodium silicate had similar enhancement on kerosene contaminated soil washing with saponin. Sodium humate can be a better choice since its application can also improve soil quality. The enhancement of sodium silicate on kerosene contaminated soil washing with Tw-80 increased with the increase of Tw-80 dosage. However, the impact of sodium chloride and sodium tartrate was opposite to sodium silicate. Sodium salts can reduce surface tension and critical micelle concentration of ionic surfactants to enhance the washing. Sodium salts can also reduce re-adsorption of oil to soil with nonionic surfactants to enhance the washing. Kerosene contamination can increase the contact angle of soil, which indicated the increase of hydrophilicity of soil. Washing with surfactants can reduce the hydrophilicitiy of soil according to contact angle measurement, which indicated that kerosene contaminated soil remediation with surfactant can also benefit nutrient and water transportation in the contaminated soil.

  1. Effect of transitioning to automatic milking systems on producers' perceptions of farm management and cow health in the Canadian dairy industry.

    PubMed

    Tse, C; Barkema, H W; DeVries, T J; Rushen, J; Pajor, E A

    2017-03-01

    Automatic milking systems (AMS), or milking robots, are becoming increasingly common, but there is little documentation of how AMS have affected farms as a whole and what challenges and benefits producers are experiencing during their transition to AMS. The objective of this national survey was to document the effect of transitioning to AMS on producer perceptions of change in housing, farm management, and cow health. In total, 217 AMS producers were surveyed from 8 Canadian provinces. Median time since transition for respondents was 30 mo. The mean number of lactating cows per robot was 51 cows, with a median of 2 AMS units per farm. Fifty-five percent of producers built a new barn to accommodate the AMS. Changing housing systems was necessary for 47% of producers, not necessary for 50%, and not applicable to 3% (as the AMS farm was their first farm). Cleaning and feeding practices remained the same. Overall, farms increased herd size from a median of 77 to 85 lactating cows with the transition to AMS. After the transition to AMS, 66% of producers changed their health-management practices. Producers reported either a decrease or no change in rate of clinical mastitis. Reports on change in rate of lameness and total bacterial count varied. Conception rate was reported to have increased for 63% of producers. Culling rate was perceived to have stayed the same for 59% of producers. Overall, producers perceived their transitions to AMS as successful. Findings from this project provide a benchmark of the effects of AMS on important aspects of Canadian dairy farming, as well as provide producers, AMS manufacturers, veterinarians, and dairy advisors with more detailed knowledge on what to expect when transitioning to AMS. Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  2. An evaluation of different soil washing solutions for remediating arsenic-contaminated soils.

    PubMed

    Wang, Yiwen; Ma, Fujun; Zhang, Qian; Peng, Changsheng; Wu, Bin; Li, Fasheng; Gu, Qingbao

    2017-04-01

    Soil washing is a promising way to remediate arsenic-contaminated soils. Most research has mostly focused on seeking efficient extractants for removing arsenic, but not concerned with any changes in soil properties when using this technique. In this study, the removal of arsenic from a heavily contaminated soil employing different washing solutions including H 3 PO 4 , NaOH and dithionite in EDTA was conducted. Subsequently, the changes in soil physicochemical properties and phytotoxicity of each washing technique were evaluated. After washing with 2 M H 3 PO 4 , 2 M NaOH or 0.1 M dithionite in 0.1 M EDTA, the soil samples' arsenic content met the clean-up levels stipulated in China's environmental regulations. H 3 PO 4 washing decreased soil pH, Ca, Mg, Al, Fe, and Mn concentrations but increased TN and TP contents. NaOH washing increased soil pH but decreased soil TOC, TN and TP contents. Dithionite in EDTA washing reduced soil TOC, Ca, Mg, Al, Fe, Mn and TP contents. A drastic color change was observed when the soil sample was washed with H 3 PO 4 or 0.1 M dithionite in 0.1 M EDTA. After adjusting the soil pH to neutral, wheat planted in the soil sample washed by NaOH evidenced the best growth of all three treated soil samples. These results will help with selecting the best washing solution when remediating arsenic-contaminated soils in future engineering applications. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Cow's milk proteins in human milk.

    PubMed

    Coscia, A; Orrù, S; Di Nicola, P; Giuliani, F; Rovelli, I; Peila, C; Martano, C; Chiale, F; Bertino, E

    2012-01-01

    Cow's milk proteins (CMPs) are among the best characterized food allergens. Cow's milk contains more than twenty five different proteins, but only whey proteins alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin (BSA), and lactoferrin, as well as the four caseins, have been identified as allergens. Aim of this study was to investigate by proteomics techniques cow's milk allergens in human colostrum of term and preterm newborns' mothers, not previously detected, in order to understand if such allergens could be cause of sensitization during lactation. Term colostrum samples from 62 healthy mothers and preterm colostrum samples from 11 healthy mothers were collected for this purpose. The most relevant finding was the detection of the intact bovine alpha-S1-casein in both term and preterm colostrum. Using this method, which allows direct proteins identification, beta-lactoglobulin was not detected in any of colostrum samples. According to our results bovine alpha 1 casein that is considered a major cow's milk allergen is readily secreted in human milk: further investigations are needed in order to clarify if alpha-1-casein has a major role in sensitization or tolerance to cow's milk of exclusively breastfed predisposed infants.

  4. Sensitive identification of mycobacterial species using PCR-RFLP on bronchial washings.

    PubMed

    Hidaka, E; Honda, T; Ueno, I; Yamasaki, Y; Kubo, K; Katsuyama, T

    2000-03-01

    In 98 patients (24 with active pulmonary tuberculosis [TB] lesions, 28 with cured TB lesions, and 46 with nontuberculous opacities [control group] in chest CT scans), we examined whether washing the bronchus after brushing the lesion, then applying polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to the bronchial washings might be useful for diagnosing TB and nontuberculous mycobacteriosis (NTMosis). After biopsy and brushing with a bronchoscope, the bronchus connecting to the lesion was washed with 20 ml saline. The saline used for washing the brushes (5 ml; brushing sample), and 3 to 10 ml saline aspirated through the forceps channel (washing sample) were examined by PCR-RFLP, which proved able to identify Mycobacterium tuberculosis and seven species of nontuberculous mycobacteria (NTM). The values obtained for the sensitivity of the PCR-RFLP with respect to the brushing sample, the washing sample, and both samples mixed together were 70, 76, and 91%, respectively, when only patients who were culture-positive or radiologically improved after antituberculous therapy were considered as showing true infection. A mixture of brushing and washing samples provides useful material for PCR and culture, and the PCR-RFLP used here is a good method for the simultaneous identification of several species of mycobacterium (including M. tuberculosis).

  5. Management of heat stress to improve fertility in dairy cows in Israel.

    PubMed

    Flamenbaum, Israel; Galon, Nadav

    2010-01-01

    Israel has about 100,000 dairy cows mostly all of Israeli-Holstein-breed, kept in close to 1000 dairy farms. Most farms are distributed along the Mediterranean Sea coast and in the hot internal valleys. According to the Israeli Herd book the average annual milk production, per cow in 2008 was 11,460 kg, with 3.7% fat and 3.2% protein. Israel's climate is considered "subtropical dry" or Mediterranean, characterized by warm and dry summer with day temperatures above 30 C and relative humidity ranging from 50 to 90%. Climatic limitations brought dairy farmers to develop and implement new technologies and management practices that would enable high milk production and reproduction in summers. In the last three decades the Ministry of Agriculture research units, the extension service and dairy farmers conducted a series of trials and surveys in order to develop an efficient cooling system that will obtain and maintain high milk yield and good reproduction during the hot and humid summer. The cooling system commonly used in Israel is based on a combination of frequent direct watering of the cows, followed by forced ventilation air blowing onto the cows. The system was developed in Israel nearly 30 years ago. A typical cycle is five minutes long and consists of 30 sec of watering followed by 4.5 min of forced ventilation. Providing the cows with 5-7 cooling sessions per day, 30-45 min each, allowed cows, producing 25-30 kg of milk per day to maintain their body temperature below 39.0 C, throughout the day time, on a typical Israeli summer day. At the same time, non-cooled cows had high body temperatures (above 39.5 C), during some part of the daytime and returned to normal body temperatures (below 39.0 C), only for a few hours late at night. In an experiment conducted in 1985-86, conception rate (CR) of cows, cooled as described above, was significantly higher than of non-cooled cows (59 vs. 17% and 57 vs. 17%), for first insemination and for all inseminations

  6. Antioxidant capacity and fatty acids characterization of heat treated cow and buffalo milk.

    PubMed

    Khan, Imran Taj; Nadeem, Muhammad; Imran, Muhammad; Ayaz, Muhammad; Ajmal, Muhammad; Ellahi, Muhammad Yaqoob; Khalique, Anjum

    2017-08-24

    Antioxidant capacity of milk is largely due to vitamins A, E, carotenoids, zinc, selenium, superoxide dismutase, catalase, glutathione peroxidase and enzyme systems. Cow milk has antioxidant capacity while the antioxidant capacity of buffalo milk has been studied in a limited way. The information regarding the effect of pasteurization and boiling on antioxidant capacity of cow and buffalo milk is also scared. Cow and buffalo milk was exposed to two different heat treatments i.e. 65 °C for 30 min and boiling for 1 min. After heat treatments, milk samples were cooled down to 4 °C packaged in transparent 250 ml polyethylene PET bottles and stored at 4 °C for 6 days. Milk composition, total flavonoid content, total antioxidant capacity, reducing power, DPPH free radical scavenging activity, antioxidant activity in linoleic acid, vitamin C, A, E, selenium, Zinc, fatty acid profile, peroxide value and sensory characteristics were studied in raw, pasteurized and boiled cow and buffalo milk at 0, 3 and 6 days of storage period. Total antioxidant capacity (TAC) of raw, pasteurized and boiled milk for cow (42.1, 41.3 and 40.7%) and buffalo (58.4, 57.6 and 56.5%) samples was found, respectively. Reducing power (RP) of raw cow and buffalo milk was 6.74 and 13.7 while pasteurization and boiling did not showed significant effect on RP of both cow and buffalo milk. DPPH activity of raw, pasteurized and boiled milk for cow (24.3, 23.8 and 23.6%) and buffalo (31.8, 31.5 and 30.4%) samples was noted, respectively. Storage period up to 3 days was non-significant while DPPH assay after 6 days of storage period indicated significant decline in antioxidant activity of milk samples. Antioxidant activity in linoleic acid (AALA) of buffalo and cow milk were recorded 11.7 and 17.4%, respectively. Pasteurization and boiling did not showed any impact on antioxidant capacity of cow and buffalo milk. The Loss of vitamin C in pasteurization (40 and 42%) and boiling (82 and 61%) of

  7. Bacterial Exchange in Household Washing Machines

    PubMed Central

    Callewaert, Chris; Van Nevel, Sam; Kerckhof, Frederiek-Maarten; Granitsiotis, Michael S.; Boon, Nico

    2015-01-01

    Household washing machines (WMs) launder soiled clothes and textiles, but do not sterilize them. We investigated the microbial exchange occurring in five household WMs. Samples from a new cotton T-shirt were laundered together with a normal laundry load. Analyses were performed on the influent water and the ingoing cotton samples, as well as the greywater and the washed cotton samples. The number of living bacteria was generally not lower in the WM effluent water as compared to the influent water. The laundering process caused a microbial exchange of influent water bacteria, skin-, and clothes-related bacteria and biofilm-related bacteria in the WM. A variety of biofilm-producing bacteria were enriched in the effluent after laundering, although their presence in the cotton sample was low. Nearly all bacterial genera detected on the initial cotton sample were still present in the washed cotton samples. A selection for typical skin- and clothes-related microbial species occurred in the cotton samples after laundering. Accordingly, malodour-causing microbial species might be further distributed to other clothes. The bacteria on the ingoing textiles contributed for a large part to the microbiome found in the textiles after laundering. PMID:26696989

  8. Bacterial Exchange in Household Washing Machines.

    PubMed

    Callewaert, Chris; Van Nevel, Sam; Kerckhof, Frederiek-Maarten; Granitsiotis, Michael S; Boon, Nico

    2015-01-01

    Household washing machines (WMs) launder soiled clothes and textiles, but do not sterilize them. We investigated the microbial exchange occurring in five household WMs. Samples from a new cotton T-shirt were laundered together with a normal laundry load. Analyses were performed on the influent water and the ingoing cotton samples, as well as the greywater and the washed cotton samples. The number of living bacteria was generally not lower in the WM effluent water as compared to the influent water. The laundering process caused a microbial exchange of influent water bacteria, skin-, and clothes-related bacteria and biofilm-related bacteria in the WM. A variety of biofilm-producing bacteria were enriched in the effluent after laundering, although their presence in the cotton sample was low. Nearly all bacterial genera detected on the initial cotton sample were still present in the washed cotton samples. A selection for typical skin- and clothes-related microbial species occurred in the cotton samples after laundering. Accordingly, malodour-causing microbial species might be further distributed to other clothes. The bacteria on the ingoing textiles contributed for a large part to the microbiome found in the textiles after laundering.

  9. The impact of aqueous washing on the ability of βFeOOH to corrode iron.

    PubMed

    Watkinson, D E; Emmerson, N J

    2017-01-01

    Controlling the corrosion of historical and archaeological ferrous metal objects presents a significant challenge to conservators. Chloride is a major corrosion accelerator in coastal areas for historic ferrous metal structures and for the many chloride-containing archaeological objects within museums. Corrosion reactions involve the formation of akaganéite (βFeOOH) which incorporates chloride within its crystal structure and adsorbs it onto its surface. The mobility of the surface-adsorbed chloride in aqueous systems and atmospheric moisture means βFeOOH can itself cause iron to corrode. The extraction of chloride from βFeOOH by aqueous Soxhlet hot wash and aqueous room temperature washing is measured. The impact of this washing on the ability of βFeOOH to corrode iron is quantitatively investigated by determining the oxygen consumption of unwashed, Soxhlet-washed and room temperature-washed samples of βFeOOH mixed with iron powder and exposed to 80 % relative humidity. This acts as a proxy measurement for the corrosion rate of iron. The results are discussed relative to climatic factors for outdoor heritage objects and the treatment of archaeological iron in museums. Delivering better understanding of the properties of βFeOOH supports the development of evidence-based treatments and management procedures in heritage conservation.

  10. Plasma-based proteomics reveals immune response, complement and coagulation cascades pathway shifts in heat-stressed lactating dairy cows.

    PubMed

    Min, Li; Cheng, Jianbo; Zhao, Shengguo; Tian, He; Zhang, Yangdong; Li, Songli; Yang, Hongjian; Zheng, Nan; Wang, Jiaqi

    2016-09-02

    Heat stress (HS) has an enormous economic impact on the dairy industry. In recent years, many researchers have investigated changes in the gene expression and metabolomics profiles in dairy cows caused by HS. However, the proteomics profiles of heat-stressed dairy cows have not yet been completely elucidated. We compared plasma proteomics from HS-free and heat-stressed dairy cows using an iTRAQ labeling approach. After the depletion of high abundant proteins in the plasma, 1472 proteins were identified. Of these, 85 proteins were differentially abundant in cows exposed to HS relative to HS-free. Database searches combined with GO and KEGG pathway enrichment analyses revealed that many components of the complement and coagulation cascades were altered in heat-stressed cows compared with HS-free cows. Of these, many factors in the complement system (including complement components C1, C3, C5, C6, C7, C8, and C9, complement factor B, and factor H) were down-regulated by HS, while components of the coagulation system (including coagulation factors, vitamin K-dependent proteins, and fibrinogens) were up-regulated by HS. In conclusion, our results indicate that HS decreases plasma levels of complement system proteins, suggesting that immune function is impaired in dairy cows exposed to HS. Though many aspects of heat stress (HS) have been extensively researched, relatively little is known about the proteomics profile changes that occur during heat exposure. In this work, we employed a proteomics approach to investigate differential abundance of plasma proteins in HS-free and heat-stressed dairy cows. Database searches combined with GO and KEGG pathway enrichment analyses revealed that HS resulted in a decrease in complement components, suggesting that heat-stressed dairy cows have impaired immune function. In addition, through integrative analyses of proteomics and previous metabolomics, we showed enhanced glycolysis, lipid metabolic pathway shifts, and nitrogen

  11. Impaired insulin signaling pathways affect ovarian steroidogenesis in cows with COD.

    PubMed

    Gareis, N C; Huber, E; Hein, G J; Rodríguez, F M; Salvetti, N R; Angeli, E; Ortega, H H; Rey, F

    2018-05-01

    Cystic ovarian disease (COD) represents an important cause of infertility in dairy cattle and is associated with multiple physiological disorders. Steroidogenesis, which is necessary to ensure normal ovarian functions, involves multiple enzymatic pathways coordinated by insulin and other proteins. We have previously shown that cows with COD have an altered insulin response. Therefore, in the present study, we evaluated further alterations in intermediates downstream of the PI3K pathway and pathways mediated by ERK as critical signals for the expression of steroidogenic enzymes in the ovaries of control cows and cows with spontaneous COD. To this end, we evaluated the gene and protein expression of pan-AKT, mTOR, ERK1/2, and steroidogenic enzymes by real-time PCR and immunohistochemistry. Steroid hormone concentrations were assessed at systemic and intrafollicular level. Results showed altered expression of intermediate molecules of the insulin signaling pathway, whose action might modify the synthetic pathway of steroidogenic hormones. Similarly, the expression of steroidogenic enzymes and the concentration of progesterone in serum and follicular fluid were altered. These alterations support the hypothesis that systemic factors contribute to the development and/or maintenance of COD, and that metabolic hormones within follicles such as insulin exert determinant effects on ovarian functionality in cows with COD. Copyright © 2018 Elsevier B.V. All rights reserved.

  12. Impact of alternative antimicrobial commercial egg washes on reducing Salmonella contamination

    USDA-ARS?s Scientific Manuscript database

    Introduction: Table eggs are washed with an alkaline detergent at approximately pH 11 and at a temperature at least 32°C, followed by a chlorine rinse. Both wash temperature and an antimicrobial rinse are required by regulation, but wash pH is not specified. At this pH, little, if any, free chlorine...

  13. Consumers' Risk Perception of Household Cleaning and Washing Products.

    PubMed

    Bearth, Angela; Miesler, Linda; Siegrist, Michael

    2017-04-01

    A large share of accidental and nonaccidental poisonings are caused by household cleaning and washing products, such as drain cleaner or laundry detergent. The main goal of this article was to investigate consumers' risk perception and misconceptions of a variety of cleaning and washing products in order to inform future risk communication efforts. For this, a sorting task including 33 commonly available household cleaning and washing products was implemented. A total of 60 female consumers were asked to place the cleaning and washing products on a reference line 3 m in length with the poles "dangerous" and "not dangerous." The gathered data were analyzed qualitatively and by means of multidimensional scaling, cluster analysis, and linear regression. The dimensionality of the sorting data suggests that both analytically (i.e., written and graphical hazard notes and perceived effectiveness) and intuitively driven risk judgments (i.e., eco vs. regular products) were applied by the participants. Furthermore, results suggest the presence of misconceptions, particularly related to consumers' perceptions of eco cleaning products, which were generally regarded as safer than their regular counterparts. Future risk communication should aim at dispelling these misconceptions and promoting accurate risk perceptions of particular household cleaning and washing products. © 2016 Society for Risk Analysis.

  14. Effects of feeding betaine-containing liquid supplement to transition dairy cows.

    PubMed

    Monteiro, A P A; Bernard, J K; Guo, J-R; Weng, X-S; Emanuele, S; Davis, R; Dahl, G E; Tao, S

    2017-02-01

    Betaine is a natural compound found in sugar beets that serves as a methyl donor and organic osmolyte when fed to animals. The objective was to evaluate the effect of feeding betaine-containing molasses on performance of transition dairy cows during late summer in 2 trials. In early September, cows were randomly assigned to betaine (BET) or control (CON) groups either shortly after dry off (trial 1; n = 10 per treatment) or 24 d before calving (trial 2; n = 8 per treatment) based on parity and previous mature equivalent milk yield. Cows were fed common diets supplemented either with a liquid supplement made of molasses from sugar cane and condensed beet solubles containing betaine [BET, 89.1 g/kg of dry matter (DM)] or a sugar cane molasses-based liquid supplement without betaine (CON) until 8 wk postpartum. The liquid supplements had similar nutrient contents and were fed at a rate of 1.1 and 1.4 kg DM/d for pre- and postpartum cows, respectively. Starting at their entry in the studies, cows were housed in the same freestall barn without a cooling system. After calving, all cows were housed in the same barn cooled by misters and fans and milked thrice daily. Intake was recorded daily and body weight and body condition score were assessed every 2 wk. Milk yield was recorded at each milking and composition was analyzed weekly. Blood samples were collected weekly from a subset of cows to assess concentrations of metabolites and AA. No treatment effects were apparent for DM intake and body weight in the prepartum and postpartum periods. For cows enrolled at dry off, BET supported higher milk yield (45.1 vs. 41.9 kg/d) and fat content (4.78 vs. 4.34%) and elevated plasma concentrations of nonesterified fatty acids and β-hydroxybutyrate in early lactation compared to CON. However, no differences were observed for milk yield, most milk component contents and yields, and blood metabolites between treatments for cows enrolled during the close-up period. Compared to cows

  15. Distillery spent wash: treatment technologies and potential applications.

    PubMed

    Mohana, Sarayu; Acharya, Bhavik K; Madamwar, Datta

    2009-04-15

    Distillery spent wash is the unwanted residual liquid waste generated during alcohol production and pollution caused by it is one of the most critical environmental issue. Despite standards imposed on effluent quality, untreated or partially treated effluent very often finds access to watercourses. The distillery wastewater with its characteristic unpleasant odor poses a serious threat to the water quality in several regions around the globe. The ever-increasing generation of distillery spent wash on the one hand and stringent legislative regulations of its disposal on the other has stimulated the need for developing new technologies to process this effluent efficiently and economically. A number of clean up technologies have been put into practice and novel bioremediation approaches for treatment of distillery spent wash are being worked out. Potential microbial (anaerobic and aerobic) as well as physicochemical processes as feasible remediation technologies to combat environmental pollution are being explored. An emerging field in distillery waste management is exploiting its nutritive potential for production of various high value compounds. This review presents an overview of the pollution problems caused by distillery spent wash, the technologies employed globally for its treatment and its alternative use in various biotechnological sectors.

  16. Determining a patient's comfort in inquiring about healthcare providers' hand-washing behavior.

    PubMed

    Clare, Camille A; Afzal, Omara; Knapp, Kenneth; Viola, Deborah

    2013-06-01

    To determine whether a patient's level of assertiveness and other factors influences her comfort level in asking her provider to wash his or her hands. In this pilot study, we developed a survey to gather cross-sectional information on a variety of factors that might explain patient willingness to ask her health-care provider to wash his or her hands. Three primary predictor variables are analyzed: (1) patient assertiveness; (2) patient familiarity with her healthcare provider; and (3) whether the patient has observed hand-washing behavior. Fifty patients participated from the Obstetrics and Gynecology Department of Metropolitan Hospital Center. Less assertive patients are much less likely than assertive patients to ask physicians to wash hands (25% versus 68%; Fisher's exact test P = 0.0427). Among the 3 assertiveness questions included in the survey, the ability to ask physicians questions during visits is most strongly indicative of willingness to ask about hand washing. Familiarity with the names of regular health-care providers has a statistically significant impact on willingness to ask about hand washing. Evidence suggests that observing hand washing behavior affects willingness to ask, but differences are not statistically significant. Results by socioeconomic status such as age, education, income, and race/ethnicity are inconclusive. A patient's level of assertiveness alone is not sufficient to determine her willingness to inquire about the hand-washing behavior of her provider. A high percentage of patients did not see their provider engaging in adequate hand washing behavior. If patients feel comfortable with their provider to inquire about their care and request hand-washing behavior, health outcomes are affected by reducing the rates of health care-associated infections.

  17. Assessing cow-calf welfare. Part 2: Risk factors for beef cow health and behavior and stockperson handling.

    PubMed

    Simon, G E; Hoar, B R; Tucker, C B

    2016-08-01

    Epidemiological studies can be used to identify risk factors for livestock welfare concerns but have not been conducted in the cow-calf sector for this purpose. The objectives of this study were to investigate the relationships of 1) herd-level management, facilities, and producer perspectives with cattle health and behavior and stockperson handling and 2) stockperson handling on cattle behavior at the individual cow level. Cow ( = 3,065) health and behavior and stockperson handling during a routine procedure (e.g., pregnancy checks) were observed on 30 California ranches. Management and producer perspectives were evaluated using an interview, and handling facility features were recorded at the chute. After predictors were screened for univariable associations, multivariable models were built for cattle health (i.e., thin body condition, lameness, abrasions, hairless patches, swelling, blind eyes, and dirtiness) and behavior (i.e., balking, vocalizing, stumbling and falling in the chute and while exiting the restraint, and running out of the restraint) and stockperson handling (i.e., electric prod use, moving aid use, tail twisting, and mis-catching cattle). When producers empathized more toward an animal's pain experience, there was a lower risk of swelling (odds ratio [OR] = 0.7) but a higher risk of lameness (OR = 1.3), which may indicate a lack of awareness of the latter. Training stockpersons using the Beef Quality Assurance program had a protective effect on cow cleanliness and mis-catching in the restraint (OR = 0.2 and OR = 0.5, respectively). Hydraulic chutes increased the risk of vocalizations (OR = 2.7), possibly because these systems can apply greater pressure to the sides of the animal than manual restraints. When a moving aid was used to move an individual cow, it increased the risk of her balking, but when hands, in particular, were used, the risk of balking decreased across the herd (OR = 34.1 and OR = 0.3, respectively). Likewise, individual cows

  18. Tips on hybridizing, washing, and scanning affymetrix microarrays.

    PubMed

    Ares, Manuel

    2014-02-01

    Starting in the late 1990s, Affymetrix, Inc. produced a commercial system for hybridizing, washing, and scanning microarrays that was designed to be easy to operate and reproducible. The system used arrays packaged in a plastic cassette or chamber in which the prefabricated array was mounted and could be filled with fluid through resealable membrane ports either by hand or by an automated "fluidics station" specially designed to handle the arrays. A special rotating hybridization oven and a specially designed scanner were also required. Primarily because of automation and standardization the Affymetrix system was and still remains popular. Here, we provide a skeleton protocol with the potential pitfalls identified. It is designed to augment the protocols provided by Affymetrix.

  19. Relationship between daily rumination time and estrus of dairy cows.

    PubMed

    Reith, S; Hoy, S

    2012-11-01

    The aim of the study was to investigate whether rumination time (RT) was related to estrus in dairy cattle. On 4 farms, cows were equipped with a microphone-based sensor system that allowed continuous recording (in blocks of 2 h) of RT. The analyzed data set consisted of 265 verified estrus cycles of 224 animals with artificial insemination leading to conception. The day of estrus (d 0) was defined as the day when estrus was identified either by measurement of physical activity or by visual observation. In estrous cows, RT was significantly reduced. With a duration of 355 min/d, the minimum RT was found on the day of estrus compared with the base level of 429 min/d during the reference period (the mean of 3 d before and 3 d after estrus). The average decrease in RT was 17% (74 min), ranging between -71 and +16% among animals. Herd and parity affected the RT decrease during estrus. Among the 4 analyzed herds, the RT decrease of cows in estrus ranged between 14% (60 min/d) and 24% (94 min/d). The decrease in RT was more pronounced in primiparous than in mature cows. In conclusion, RT is reduced on the day of estrus on average. The RT decrease during estrus was characterized by high variation among cows. Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  20. Marine worms (genus Osedax) colonize cow bones.

    PubMed

    Jones, William J; Johnson, Shannon B; Rouse, Greg W; Vrijenhoek, Robert C

    2008-02-22

    Bone-eating worms of the genus Osedax colonized and grew on cow bones deployed at depths ranging from 385 to 2893m in Monterey Bay, California. Colonization occurred as rapidly as two months following deployment of the cow bones, similar to the time it takes to colonize exposed whalebones. Some Osedax females found on the cow bones were producing eggs and some hosted dwarf males in their tubes. Morphological and molecular examinations of these worms confirmed the presence of six Osedax species, out of the eight species presently known from Monterey Bay. The ability of Osedax species to colonize, grow and reproduce on cow bones challenges previous notions that these worms are 'whale-fall specialists.'

  1. Washing when the sun is shining! How users interact with a household energy management system.

    PubMed

    Kobus, Charlotte B A; Mugge, Ruth; Schoormans, Jan P L

    2013-01-01

    To make optimal use of sustainable energy, domestic electricity consumption should shift to match local supply conditions. Energy management systems (EMS) are a new sustainable technology that can help to disrupt consumers' habits concerning electricity consumption, whilst reinforcing desired behaviours. This research examined the factors that influence the likelihood that people will shift their electricity consumption to match sustainable supply. Twenty-one interviews were conducted with households who had used the EMS 'Smart Wash' for several months. The findings showed that the likelihood of behaviour change is influenced by a combination of the user's motivation, specific contextual factors and the design of the EMS. Based on these results, several recommendations are given for the future design of EMSs. Energy management systems (EMS) are a new technology that encourages people to shift electricity consumption to match local solar supply. Interviews among users of an EMS showed that the likelihood of behaviour change is influenced by the combination of the user's motivation, contextual factors and the EMS design.

  2. Calm temperament improves reproductive performance of beef cows.

    PubMed

    Kasimanickam, R; Asay, M; Schroeder, S; Kasimanickam, V; Gay, J M; Kastelic, J P; Hall, J B; Whittier, W D

    2014-12-01

    Profitability of a beef operation is determined by the proportion of cows attaining pregnancy early in the breeding season and those that are pregnant at the end of breeding season. Many factors, including temperament, contribute to those reproductive parameters. The objective of this study was to evaluate effects of temperament on reproductive performance of beef cows. In Experiment 1, Angus and Angus-cross beef cows (n = 1546) from eight locations were assigned a body condition score (BCS; 1 = emaciated; 9 = obese) and chute exit and gait score (1 = slow exit, walk; calm temperament; 2 = jump, trot or run; excitable temperament). Cows were grouped with bulls (1 : 25 to 1 : 30; with satisfactory breeding potential and free of venereal disease) for an 85-day breeding season. Pregnancy status and stage of gestation were determined (transrectal palpation) 35 days after the end of the breeding season. Controlling for BCS (p < 0.01) and handling facility (p < 0.0001) and handling facility by temperament score interaction (p < 0.001), breeding season pregnancy rate was lower in excited versus calm cows [88.6% (798/901) vs 94.1% (607/645); p < 0.001]. Cows with an excitable temperament took 24 more days to become pregnant compared to calm cows (median days to pregnancy, 35 vs 59 days; p < 0.0001). In Experiment 2, Angus and Angus-cross beef cows (n = 1407) from 8 locations were assigned scores for body condition and chute exit and gait (as described in Experiment 1) and assigned to bulls (breeding sound and free of venereal disease; 1 : 25 to 1 : 30) for 85 days. Pregnancy status was determined by transrectal palpation at 2 and 6 months after the onset of the breeding season. Controlling for BCS (p < 0.05), pregnancy loss was higher in excited versus calm cows [5.5% (36/651) vs 3.2% (20/623), p < 0.0001]. In conclusion, beef cows with an excitable temperament had significantly lower reproductive performance than calmer cows. The modified two-point chute exit-gait scoring

  3. A field study on the effects of dietary monensin on milk production and milk composition in dairy cows

    PubMed Central

    Dubuc, Jocelyn; DuTremblay, Denis; Baril, Jean; Bagg, Randy; Brodeur, Marcel; Duffield, Todd; DesCôteaux, Luc

    2010-01-01

    The objectives of this study were to quantify the effect of 16 ppm of dietary monensin on milk production and composition of dairy cows, and to investigate factors having a potential impact on this effect. Data were generated from a total of 3577 Holstein dairy cows (47 herds) in Quebec enrolled in a herd-level, randomized clinical trial investigating the effects of monensin supplementation. Milk production and composition data were collected from monthly dairy herd improvement (DHI) testing. Monensin increased milk production by 0.9 kg/cow/d in cows under 150 days in milk (DIM) (P < 0.05). Monensin decreased milk fat percentage by 0.18 percentage points during the whole lactation (P < 0.05). This decreasing effect was larger for component-fed cows (P < 0.05) and for cows being fed low levels of dietary physically effective particles (P < 0.05) when compared respectively to cows fed total mixed ration and cows fed high levels of dietary physically effective particles. The results of this study suggest that monensin influences milk production and milk composition of dairy cows, and that diet composition and feeding system influence those effects. PMID:20592825

  4. 33 CFR 155.710 - Qualifications of person in charge.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... SECURITY (CONTINUED) POLLUTION OIL OR HAZARDOUS MATERIAL POLLUTION PREVENTION REGULATIONS FOR VESSELS... control and monitoring systems, the procedures for reporting pollution incidents, and, if installed, the Crude-Oil Washing (COW), inert-gas, and vapor-control systems—to safely conduct a transfer of fuel oil...

  5. 33 CFR 155.710 - Qualifications of person in charge.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... SECURITY (CONTINUED) POLLUTION OIL OR HAZARDOUS MATERIAL POLLUTION PREVENTION REGULATIONS FOR VESSELS... control and monitoring systems, the procedures for reporting pollution incidents, and, if installed, the Crude-Oil Washing (COW), inert-gas, and vapor-control systems—to safely conduct a transfer of fuel oil...

  6. Enhanced salmonella reduction on tomatoes washed in chlorinated water with wash aid T-128

    USDA-ARS?s Scientific Manuscript database

    Chlorine is widely used by the fresh and fresh-cut produce industries to reduce microbial populations and to prevent potential pathogen cross contamination during produce washing. However, the organic materials released from produce quickly react with chlorine and degrade its efficacy for pathogen i...

  7. Relationship between Escherichia coli virulence factors and postpartum metritis in dairy cows.

    PubMed

    Kassé, F N; Fairbrother, J M; Dubuc, J

    2016-06-01

    The objectives of this study were to report the prevalence of Escherichia coli and Trueperella pyogenes in the uterus of postpartum dairy cows before the onset of postpartum metritis (PPM) and to quantify their association with subsequent occurrence of PPM, to quantify the association between the presence of genes encoding E. coli virulence factors (VF) and PPM, and to determine the accuracy of using early postpartum uterine bacteriology results (bacteria and VF) to identify cows at risk of PPM. A prospective cohort study was conducted on 3 commercial dairy farms. Uterine swabs were collected from 371 Holstein dairy cows (3 commercial herds) at 1 to 7d in milk and submitted to the laboratory for identification of E. coli, T. pyogenes, and E. coli VF. A total of 40 VF were tested using the radioactive probe hybridization method. Postpartum metritis was defined as the presence of a fetid watery red-brown uterine discharge, associated with fever (rectal temperature >39.5°C), and systemic signs of illness (dullness, reduced appetite, and milk production). Surveillance of PPM was done by trained farmers blinded to laboratory results and cows were followed until 21d in milk. Statistical analyses were conducted using 2×2 tables and mixed logistical regression models. Prevalences of E. coli, T. pyogenes, and PPM were 42, 34, and 15%, respectively. A total of 32 VF were found in E. coli isolates. Most prevalent VF were extraintestinal pathogenic genes such as fimH (89%), hlyE (87%), and iss (70%). Cows positive for intrauterine E. coli were 3.2 times more likely to have subsequent PPM compared with bacteriologically negative cows. Cows with VF hra1 in their uterus were 2.7 times more likely to have PPM than cows positive for E. coli and negative for hra1 and 5.9 times more likely than bacteriologically negative cows. Cows with VF kpsMTII in their uterus were 3.2 times more likely to have PPM than cows positive for E. coli and negative for kpsMTII and 6.2 times more likely

  8. 6. GENE WASH DAM, LOOKING NORTHWEST. SURVEY REFLECTOR IN FOREGROUND ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. GENE WASH DAM, LOOKING NORTHWEST. SURVEY REFLECTOR IN FOREGROUND FOR MONITORING MOVEMENT OF DAM AND EARTH. - Gene Wash Reservoir & Dam, 2 miles west of Parker Dam, Parker Dam, San Bernardino County, CA

  9. Face washing promotion for preventing active trachoma

    PubMed Central

    Ejere, Henry OD; Alhassan, Mahmoud B; Rabiu, Mansur

    2015-01-01

    Background Trachoma remains a major cause of avoidable blindness among underprivileged populations in many developing countries. It is estimated that about 146 million people have active trachoma and nearly six million people are blind due to complications associated with repeat infections. Objectives The objective of this review was to assess the effects of face washing promotion for the prevention of active trachoma in endemic communities. Search methods We searched CENTRAL (which contains the Cochrane Eyes and Vision Group Trials Register) (2015, Issue 1), Ovid MEDLINE, Ovid MEDLINE In-Process and Other Non-Indexed Citations, Ovid MEDLINE Daily, Ovid OLDMEDLINE (January 1946 to January 2015), EMBASE (January 1980 to January 2015), PubMed (January 1948 to January 2015), Latin American and Caribbean Health Sciences Literature Database (LILACS) (January 1982 to January 2015), the metaRegister of Controlled Trials (mRCT) (www.controlled-trials.com) (accessed 10 January 2014), ClinicalTrials.gov (www.clinicaltrials.gov) and the World Health Organization (WHO) International Clinical Trials Registry Platform (ICTRP) (www.who.int/ictrp/search/en). We did not use any date or language restrictions in the electronic searches for trials. We last searched the electronic databases on 26 January 2015. To identify further relevant trials we checked the reference lists of the included trials. Also, we used the Science Citation Index to search for references to publications that cited the trials included in the review. We contacted investigators and experts in the field to identify additional trials. Selection criteria We included randomized controlled trials (RCTs) or quasi-RCTs that compared face washing with no treatment or face washing combined with antibiotics against antibiotics alone. Trial participants were residents of endemic trachoma communities. Data collection and analysis Two review authors independently extracted data and assessed trial quality. We contacted trial

  10. Wash-out in N{sub 2}-dominated leptogenesis

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hahn-Woernle, F., E-mail: fhahnwo@mppmu.mpg.de

    2010-08-01

    We study the wash-out of a cosmological baryon asymmetry produced via leptogenesis by subsequent interactions. Therefore we focus on a scenario in which a lepton asymmetry is established in the out-of-equilibrium decays of the next-to-lightest right-handed neutrino. We apply the full classical Boltzmann equations without the assumption of kinetic equilibrium and including all quantum statistical factors to calculate the wash-out of the lepton asymmetry by interactions of the lightest right-handed state. We include scattering processes with top quarks in our analysis. This is of particular interest since the wash-out is enhanced by scatterings and the use of mode equations withmore » quantum statistical distribution functions. In this way we provide a restriction on the parameter space for this scenario.« less

  11. Effect of uterine size on fertility of lactating dairy cows.

    PubMed

    Baez, Giovanni M; Barletta, Rafael V; Guenther, Jerry N; Gaska, Jerry M; Wiltbank, Milo C

    2016-05-01

    There are multiple reasons for reduced fertility in lactating dairy cows. We hypothesized that one cause of reduced fertility could be the overall size of the reproductive tract, particularly the uterus, given well-established uterine functions in many aspects of the reproductive process. Thus, the objectives of this study were to evaluate the variability in uterine size in primiparous and multiparous dairy cows and to analyze whether there was an association between uterine size and fertility, particularly within a given parity. Lactating Holstein dairy cows (n = 704) were synchronized to receive timed artificial insemination (TAI) on Day 81 ± 3 of lactation by using the Double-Ovsynch protocol (GnRH-7d-PGF-3d-GnRH-7d-GnRH-7d-PGF-56h-GnRH-16h-TAI). At the time of the last injection of PGF, uterine diameter was determined at the greater curvature using ultrasound, uterine length was determined by rectal palpation, and uterine volume was calculated from these two measurements. Blood samples were also taken to measure progesterone to assure synchronization of all cows used in the final analysis (n = 616; primiparous, n = 289; multiparous, n = 327). Primiparous cows had greater percentage pregnant/AI (P/AI) compared to multiparous cows (49.8% vs. 39.1% at 67 days of pregnancy diagnosis, P = 0.009). Diameter, length, and volume of the uterus were larger in multiparous than in primiparous cows (P < 0.001). For multiparous cows, uterine diameter and volume were smaller in cows that became pregnant compared to cows that were not pregnant to the TAI with a similar tendency observed in primiparous cows. Logistic regression and quartile analysis also showed that as uterine volume increased, there was decreased P/AI in either primiparous or multiparous cows. Thus, there is a negative association between uterine size and fertility in lactating dairy cows with a larger uterus associated with reduced fertility, particularly for multiparous cows. Copyright

  12. Proteomic content of circulating exosomes in dairy cows with or without uterine infection.

    PubMed

    Almughlliq, Fatema B; Koh, Yong Q; Peiris, Hassendrini N; Vaswani, Kanchan; McDougall, Scott; Graham, Elizabeth M; Burke, Chris R; Arachchige, Buddhika J; Reed, Sarah; Mitchell, Murray D

    2018-07-01

    In the past few decades, there has been a global decrease in dairy cow reproductive performance. An activated inflammatory system, due to uterine infection, has been associated with decreased cow fertility and as such, there is a need to detect uterine disease earlier. Early detection could be achieved by identifying biomarkers for uterine disease. Exosomes are small nanovesicles known to package and deliver protein, mRNA, and miRNAs to near and distant sites. Therefore, the content of circulating exosomes may have the potential to carry biomarkers for earlier diagnosis of disease. We hypothesized that circulating exosomes from cows with and without uterine infection may contain information representative of endometrial health or disease. We compared the proteomic content of circulating exosomes derived from plasma of dairy cows with (n = 10) or without (n = 10) induced uterine infection, using high-performance liquid chromatography tandem mass spectrometry (HPLC MS/MS). Our results demonstrate that there were a total of 103 bovine and 9 Trueperella pyogenes proteins found in plasma exosomes derived from infected cows (infected exosomes), and 90 bovine and 5 T. pyogenes proteins found in exosomes derived from plasma of non-infected cows (non-infected exosomes). 71 bovine proteins were found to be unique to the infected exosomes while only 4 bovine proteins were found to be unique to the non-infected exosomes. 8 unique T. pyogenes proteins were identified in infected exosomes and 4 were found to be unique to the non-infected exosomes. Pathway analysis showed that infected exosomes had more proteins involved in structural molecule activity and immune system processes than non-infected exosomal protein. Additionally, proteins from infected exosomes were involved in unique pathways: angiogenesis and integrin signaling pathway. Our data provide preliminary evidence of a potential role for exosomes in the early diagnosis of uterine infection in dairy cows

  13. Udder health of dairy cows fed different dietary energy levels after a short or no dry period without use of dry cow antibiotics.

    PubMed

    van Hoeij, R J; Lam, T J G M; Bruckmaier, R M; Dijkstra, J; Remmelink, G J; Kemp, B; van Knegsel, A T M

    2018-05-01

    Reports on the effects of length of dry period (DP) on udder health of cows that were not treated with dry cow antibiotics are scarce. Additionally, the effects of a reduced dietary energy level for cows with a 0-d DP on udder health have not yet been studied. The aims of this study were (1) to compare effects of a 0-d or 30-d DP without use of dry cow antibiotics on udder health across the DP and subsequent lactation in dairy cows fed different dietary energy levels and (2) to evaluate associations between udder health and metabolic status of dairy cows. Five weeks before the expected calving date, Holstein-Friesian dairy cows (n = 115) were blocked for parity, expected calving date, and milk yield and somatic cell count (SCC) at their 2 last test days and were randomly assigned to 2 DP lengths: 0-d DP (n = 77) or 30-d DP (n = 38). Quarter milk samples were taken in wk 5 prepartum and in wk 1 and 5 postpartum. Proportion of quarters with elevated SCC (SCC ≥200,000 cells/mL) and proportion of udder pathogens in quarter milk samples did not differ between DP lengths among weeks. After calving, 102 of these cows were randomly assigned to 3 treatments: a 30-d DP with a standard energy level required for expected milk yield (30-d DP SEL; n = 36), a 0-d DP with the same energy level as cows with a 30-d DP (0-d DP SEL; n = 33), and a 0-d DP with a low energy level (0-d DP LEL, n = 33). From wk 8 of lactation onward, cows received either a glucogenic ration consisting of corn silage and grass silage or a lipogenic ration consisting of grass silage and sugar beet pulp at a standard or low energy level. During wk 1 to 7 postpartum, treatment did not affect SCC or SCC corrected for milk yield. During wk 8 to 44 of lactation, 0-d DP SEL cows had a greater SCC than 0-d DP LEL or 30-d DP SEL cows and had a greater SCC corrected for milk yield than 0-d DP LEL cows. During wk 1 to 44 of lactation, occurrence of at least 1 elevation of SCC (SCC ≥200,000 cells/mL after 2 wk of

  14. Allergenicity of mare's milk in children with cow's milk allergy.

    PubMed

    Businco, L; Giampietro, P G; Lucenti, P; Lucaroni, F; Pini, C; Di Felice, G; Iacovacci, P; Curadi, C; Orlandi, M

    2000-05-01

    Cow's milk allergy is a common disease of infancy and early childhood. If the baby is not breast-fed, a substitute for cow's milk formula is necessary. The aim of this study was to investigate, in vitro and in vivo, the allergenicity of mare's milk in a population of selected children with severe IgE-mediated cow's milk allergy. Twenty-five children (17 male and 8 female) aged 19 to 72 months (median age 34 months) with IgE-mediated cow's milk allergy were selected for this study. All the children underwent skin prick tests with cow's milk and mare's milk and double-blind placebo-controlled oral food challenge (DBPCOFC) with fresh cow's milk, fresh mare's milk, and, as placebo, a soy formula (Isomil, Abbott, Campoverde, Italy). We performed immunoblotting of cow's and mare's milk developed with IgE from allergic children. All the children showed strong positive skin test responses to cow's milk (4+); 2 children had positive skin test responses to mare's milk (2+). All children had positive DBPCOFCs to cow's milk; one child had a positive DBPCOFC to mare's milk. No children reacted to the placebo (Isomil). In the cow's milk, some proteins are able to strongly react with human IgE; when the sera are tested with mare's milk, the bands corresponding to the same proteins are recognized by a lower percentage of sera. These data suggest that mare's milk can be regarded as a good substitute of cow's milk in most children with severe IgE-mediated cow's milk allergy. It would be prudent, however, to confirm its tolerability by a supervised titrated oral challenge test.

  15. Wake wash waves produced by High Speed Crafts:measurements vs prediction

    NASA Astrophysics Data System (ADS)

    Benassai, Guido

    2010-05-01

    The subject of this study refers to the wake wash waves generated by High Speed Crafts observed at some distance away (typically one or multiple of ship lengths) from the line of travel of the vessel. The ratio of the vessel speed divided by the maximum wave celerity in shallow water (depth-based Froude number) or to the square root of the gravity by the vessel length (length-based Froude number) is often used to classify the wash. In fact the wash waves produced by vessels that travel at sub-critical Froude numbers are different in patterns (and hence applicable theory) from that produced by vessels which operate at the critical Froude number of 1 or at supercritical Froude numbers. High Speed Crafts generally operate at Fr>1, even if in some cases for safety of navigation they operate at Fr<1. In the study supercritical speed conditions were considered. The predicted wake wash was a result of a desk-top study and relied on the subject matter presented in numerous technical papers and publications, while the measured wake wash is a result of the first field measurements of wake wash produced by HSC operating in the Bay of Naples. The measurements were operated by a pressure gauge in three critical points where the distance from the coastline was less than 700m. These measurements were taken in shallow water (depth ranging from 4 to 5 meters) in calm weather conditions. The output of the tests were wave-elevation time histories upon which the maximum wave height Hm from the wave record was extracted. The wave height reported was therefore the highest wave, peak to through, which occurred in a wave train. The wave period is defined as double the related half period for the defined maximum wave height. For each wake wash measurement the vessel route was monitored aboard the crossing HSC and exact speed, distance and water obtained depth was determined. The obtained values of the wake wash were compared with predictions of wake wash obtained by similar vessels in

  16. Nutrient Restriction Increases Circulating and Hepatic Ceramide in Dairy Cows Displaying Impaired Insulin Tolerance.

    PubMed

    Davis, Amanda N; Clegg, J L; Perry, C A; McFadden, J W

    2017-09-01

    The progression of insulin resistance in dairy cows represents a maternal adaptation to support milk production during heightened energy demand; however, excessive adipose tissue lipolysis can develop. In diabetic non-ruminants, the mechanisms that mediate insulin resistance involve the sphingolipid ceramide. We tested the hypothesis that ceramide accumulates in dairy cows experiencing lipolysis and insulin resistance. Nine dairy cows were utilized in a replicated 3 × 3 Latin square design. Cows were ad libitum fed, nutrient-restricted (NR), or NR with nicotinic acid (NA; 5 mg of NA/h per kg BW; delivered i.v.) for 34 h. When provided access, cows were ad libitum fed a mixed ration of grass hay and ground corn to meet requirements. Intake for NR cows was limited to vitamins and minerals. Nicotinic acid was administered to suppress lipolysis. Saline was infused in cows not provided NA. At 32 and 33 h of treatment, a liver biopsy and insulin tolerance test were performed, respectively. Samples were analyzed using colorimetry, immunoassay, and mass spectrometry. Nutrient restriction increased serum fatty acids and ceramide levels, and impaired insulin sensitivity; however, NA infusion was unable to prevent these responses. We also show that NR increases hepatic ceramide accumulation, a response that was positively associated with serum ceramide supply. Our data demonstrate that circulating and hepatic 24:0-Cer are inversely associated with systemic insulin tolerance, an effect not observed for the 16:0 moiety. In conclusion, our results suggest that ceramide accrual represents a metabolic adaptation to nutrient restriction and impaired insulin action in dairy cows.

  17. [Incidence of IgE-mediated allergy to cow's milk proteins in the first year of life].

    PubMed

    Sanz Ortega, J; Martorell Aragonés, A; Michavila Gómez, A; Nieto García, A

    2001-06-01

    To study the incidence of IgE-mediated allergy to cow's milk proteins during the first year of life. A multicenter, prospective study of newborns selected from different health centers was performed. The newborn infants were followed-up during the first year of life. Newborns with suspected adverse reaction to cow's milk were sent to the referral hospital for diagnostic study. This study was based on clinical history, skin tests (skin prick test) and on determination of specific IgE in serum (Pharmacia CAP system) against cow's milk and its protein fractions. Diagnosis was confirmed by open challenge. A total of 1,663 newborns were followed-up during the first year of life. Adverse reaction was suspected in 56 infants (3.3%). Allergy to cow's milk proteins was confirmed in 6 infants (0.36 %). Eighty-three percent of (5/6) children with cow's milk allergy had first-degree relatives with atopic disease compared with 19 % of children (329/1657) without cow's milk allergy. Among the entire sample, 26 infants had first-degree relatives with atopic disease and one of these infants (3.8%) developed cow milk allergy. The six children with cow's milk allergy were exclusively breast-fed, and clinical reaction developed within 1 week of the introduction of artificial feeding. The incidence of IgE-mediated allergy to cow's milk was 0.36 %. In infants with two first-degree family members with atopic disease, the probability of developing allergy to cow's milk proteins during the first year of life was 3.8%.

  18. A model to estimate insulin sensitivity in dairy cows.

    PubMed

    Holtenius, Paul; Holtenius, Kjell

    2007-10-11

    Impairment of the insulin regulation of energy metabolism is considered to be an etiologic key component for metabolic disturbances. Methods for studies of insulin sensitivity thus are highly topical. There are clear indications that reduced insulin sensitivity contributes to the metabolic disturbances that occurs especially among obese lactating cows. Direct measurements of insulin sensitivity are laborious and not suitable for epidemiological studies. We have therefore adopted an indirect method originally developed for humans to estimate insulin sensitivity in dairy cows. The method, "Revised Quantitative Insulin Sensitivity Check Index" (RQUICKI) is based on plasma concentrations of glucose, insulin and free fatty acids (FFA) and it generates good and linear correlations with different estimates of insulin sensitivity in human populations. We hypothesized that the RQUICKI method could be used as an index of insulin function in lactating dairy cows. We calculated RQUICKI in 237 apparently healthy dairy cows from 20 commercial herds. All cows included were in their first 15 weeks of lactation. RQUICKI was not affected by the homeorhetic adaptations in energy metabolism that occurred during the first 15 weeks of lactation. In a cohort of 24 experimental cows fed in order to obtain different body condition at parturition RQUICKI was lower in early lactation in cows with a high body condition score suggesting disturbed insulin function in obese cows. The results indicate that RQUICKI might be used to identify lactating cows with disturbed insulin function.

  19. Marine worms (genus Osedax) colonize cow bones

    PubMed Central

    Jones, William J; Johnson, Shannon B; Rouse, Greg W; Vrijenhoek, Robert C

    2007-01-01

    Bone-eating worms of the genus Osedax colonized and grew on cow bones deployed at depths ranging from 385 to 2893 m in Monterey Bay, California. Colonization occurred as rapidly as two months following deployment of the cow bones, similar to the time it takes to colonize exposed whalebones. Some Osedax females found on the cow bones were producing eggs and some hosted dwarf males in their tubes. Morphological and molecular examinations of these worms confirmed the presence of six Osedax species, out of the eight species presently known from Monterey Bay. The ability of Osedax species to colonize, grow and reproduce on cow bones challenges previous notions that these worms are ‘whale-fall specialists.’ PMID:18077256

  20. 21 CFR 1210.12 - Physical examination of cows.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Physical examination of cows. 1210.12 Section 1210... FEDERAL IMPORT MILK ACT Inspection and Testing § 1210.12 Physical examination of cows. (a) Physical examination of any and all cows in herds producing milk or cream which is to be shipped or transported into...

  1. Assessment of an improved bone washing protocol for deceased donor human bone.

    PubMed

    Eagle, M J; Man, J; Rooney, P; Hogg, P; Kearney, J N

    2015-03-01

    NHSBT Tissue Services issues bone to surgeons in the UK in two formats, fresh-frozen unprocessed bone from living donors and processed bone from deceased donors. Processed bone may be frozen or freeze dried and all processed bone is currently subjected to a washing protocol to remove blood and bone marrow. In this study we have improved the current bone washing protocol for cancellous bone and assessed the success of the protocol by measuring the removal of the bone marrow components: soluble protein, DNA and haemoglobin at each step in the process, and residual components in the bone at the end of the process. The bone washing protocol is a combination of sonication, warm water washes, centrifugation and chemical (ethanol and hydrogen peroxide) treatments. We report that the bone washing protocol is capable of removing up to 99.85 % soluble protein, 99.95 % DNA and 100 % of haemoglobin from bone. The new bone washing protocol does not render any bone cytotoxic as shown by contact cytotoxicity assays. No microbiological cell growth was detected in any of the wash steps. This process is now in use for processed cancellous bone issued by NHSBT.

  2. 4. AERIAL VIEW OF GENE WASH RESERVOIR AND GENE CAMP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. AERIAL VIEW OF GENE WASH RESERVOIR AND GENE CAMP LOOKING SOUTHWEST. DAM AND SPILLWAY VISIBLE IN BOTTOM OF PHOTO. - Gene Wash Reservoir & Dam, 2 miles west of Parker Dam, Parker Dam, San Bernardino County, CA

  3. Economic feasibility of cooling dry cows across the United States.

    PubMed

    Ferreira, F C; Gennari, R S; Dahl, G E; De Vries, A

    2016-12-01

    Heat stress during the dry period reduces milk yield in the subsequent lactation of dairy cows. Our objectives were to quantify the economic losses due to heat stress if dry cows are not cooled and to evaluate the economic feasibility of dry cow cooling. We used weather data from the National Oceanic and Atmospheric Administration to calculate the number of heat stress days for each of the 50 US states. A heat stress day was declared when the daily average temperature-humidity index was ≥68. The number of dairy cows in each state in 2015 was obtained from the USDA-National Agricultural Statistics Service. We assumed that 15% of the cows were dry at any time, a 60-d dry period, and a calving interval of 400d. Only cows in their second or greater parity (65%) benefitted from cooling during the dry period of the previous parity. Milk yield decreased by 5kg in the subsequent lactation (340d) if the cow experienced heat stress during the dry period based on a review of the literature. The default marginal value of milk minus feed cost was $0.33/kg of milk. The investment analysis included purchases of fans and soakers and use of water and electricity. Investment in a dry cow barn was considered separately. The average US dairy cow would experience 96 (26%) heat stress days during the year if not cooled and loses 447kg of milk in the subsequent lactation if not cooled when dry. Annual losses would be $810 million if dry cows were not cooled ($87/cow per yr). For the top 3 milk-producing states (California, Wisconsin, New York), and Florida and Texas, the average milk losses in the subsequent lactation were 522, 349, 387, 1,197, and 904kg, and reduced profit per cow per year would be $101, $68, $75, $233, and $176, respectively. The average benefit-cost ratio and payback periods of cooling dry cows in the United States were 3.15 and 0.27 yr (dry cow barn already present) and 1.45 and 5.68 yr (if investing in a dry cow barn) in the default scenario. To reach positive net

  4. Dairy cow preference for different types of outdoor access.

    PubMed

    Smid, Anne-Marieke C; Weary, Daniel M; Costa, Joao H C; von Keyserlingk, Marina A G

    2018-02-01

    Dairy cows display a partial preference for being outside, but little is known about what aspects of the outdoor environment are important to cows. The primary aim of this study was to test the preference of lactating dairy cattle for pasture versus an outdoor sand pack during the night. A secondary aim was to determine whether feeding and perching behavior changed when cows were provided outdoor access. A third objective was to investigate how the lying behavior of cows changed when given access to different outdoor areas. Ninety-six lactating pregnant cows were assigned to 8 groups of 12 animals each. After a baseline phase of 2 d in which cows were kept inside the freestall barn, cows were habituated to the outdoor areas by providing them access to each of the 2 options for 24 h. Cows were then given access, in random order by group, to either the pasture (pasture phase) or the sand pack (sand phase). As we tested the 2 outdoor options using space allowances consistent with what would be practical on commercial dairy farms, the space provided on pasture was larger (21,000 m 2 ) than that provided on the sand pack (144 m 2 ). Cows were tested at night (for 2 nights in each condition), from 2000 h until morning milking at approximately 0800 h, as preference to be outdoors is strongest at this time. During the next 3 nights cows were given access to both outside options simultaneously (choice phase). Feeding and perching behaviors were recorded when cows were indoors during the day and night periods. Lying behavior was automatically recorded by HOBO data loggers (Onset, Bourne, MA). Cows spent more time outside in the pasture phase (90.0 ± 5.9%) compared with the sand phase (44.4 ± 6.3%). When provided simultaneous access to both options, cows spent more time on pasture than on the sand pack (90.5 ± 2.6% vs. 0.8 ± 0.5%, respectively). Time spent feeding indoors during the day did not change regardless of what type of outdoor access was provided, but there was a

  5. 33 CFR 117.965 - Cow Bayou.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Cow Bayou. 117.965 Section 117.965 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Texas § 117.965 Cow Bayou. The draws of the Orange County...

  6. Effect of silage from ryegrass intercropped with winter or common vetch for grazing dairy cows in small-scale dairy systems in Mexico.

    PubMed

    Hernández-Ortega, Martha; Heredia-Nava, Darwin; Espinoza-Ortega, Angelica; Sánchez-Vera, Ernesto; Arriaga-Jordán, Carlos M

    2011-06-01

    The objective was to determine the effect of including silages of annual ryegrass (Lolium multiflorum) intercropped with winter vetch (Vicia villosa) (ARG-VV) or with common vetch (Vicia sativa) (ARG-VS) compared with maize silage (MS) on milk yield and milk composition of dairy cows grazing cultivated perennial ryegrass-white clover pastures with supplemented concentrate during the dry season. Six Holstein dairy cows with a mean yield of 19.0 kg/cow/day at the beginning of the experiment were randomly assigned to a 3 × 3 repeated Latin square. Treatments were: 8 h/day intensive grazing, 3.6 kg of dry matter (DM) per cow per day of concentrate plus MS, and ARG-VV or ARG-VS ad libitum at a stocking rate of 3.0 cows/ha for three experimental periods of 3 weeks each. Milk yield (MY) and milk composition, live weight and body condition score as well as silage and concentrate intakes were recorded during the third week of each experimental period, and pasture intake was estimated indirectly from utilised metabolisable energy. Economic analysis was obtained by preparing partial budgets. There were no statistical differences (P > 0.10) in MY, milk fat or protein content nor for live weight, but there was significant difference (P < 0.10) in body condition score. There were non-statistical differences in silage DM intake (P < 0.11); however, significant differences (P < 0.10) were obtained for estimated grazed herbage intake whilst no differences for total DM intake. Slightly higher economic returns (10%) were obtained with ARG-VS over MS, and this was 7% higher than ARG-VV. It is concluded that ARG-VS could be an option for complementing grazing for small-scale dairy production systems in the dry season as it is comparable to MS in animal performance and slightly better in economic terms.

  7. Fresh-cow handling practices and methods for identification of health disorders on 45 dairy farms in California.

    PubMed

    Espadamala, A; Pallarés, P; Lago, A; Silva-Del-Río, N

    2016-11-01

    variation in signs of health disorders screened and how those signs were evaluated. Fresh cows were primarily evaluated based on nonspecific and subjective observations during screening. Future research efforts should focus on developing and validating scoring systems to more objectively identify health disorders in postpartum cows. Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  8. Increasing serotonin concentrations alter calcium and energy metabolism in dairy cows.

    PubMed

    Laporta, Jimena; Moore, Spencer A E; Weaver, Samantha R; Cronick, Callyssa M; Olsen, Megan; Prichard, Austin P; Schnell, Brian P; Crenshaw, Thomas D; Peñagaricano, Francisco; Bruckmaier, Rupert M; Hernandez, Laura L

    2015-07-01

    A 4×4 Latin square design in which varied doses (0, 0.5, 1.0, and 1.5 mg/kg) of 5-hydroxy-l-tryptophan (5-HTP, a serotonin precursor) were intravenously infused into late-lactation, non-pregnant Holstein dairy cows was used to determine the effects of serotonin on calcium and energy metabolism. Infusion periods lasted 4 days, with a 5-day washout between periods. Cows were infused at a constant rate for 1 h each day. Blood was collected pre- and 5, 10, 30, 60, 90, and 120 min post-infusion, urine was collected pre- and post-infusion, and milk was collected daily. All of the 5-HTP doses increased systemic serotonin as compared to the 0 mg/kg dose, and the 1.0 and 1.5 mg/kg doses increased circulating glucose and non-esterified fatty acids (NEFA) and decreased beta-hydroxybutyrate (βHBA) concentrations. Treatment of cows with either 1.0 or 1.5 mg/kg 5-HTP doses decreased urine calcium elimination, and the 1.5 mg/kg dose increased milk calcium concentrations. No differences were detected in the heart rates, respiration rates, or body temperatures of the cows; however, manure scores and defecation frequency were affected. Indeed, cows that received 5-HTP defecated more, and the consistency of their manure was softer. Treatment of late-lactation dairy cows with 5-HTP improved energy metabolism, decreased loss of calcium into urine, and increased calcium secretion into milk. Further research should target the effects of increasing serotonin during the transition period to determine any benefits for post-parturient calcium and glucose metabolism. © 2015 Society for Endocrinology.

  9. Eating and rumination behaviour in cows with traumatic reticuloperitonitis.

    PubMed

    Braun, U; Tschoner, T; Hässig, M; Nuss, K

    2017-02-01

    Eating and rumination behaviour in 22 cows with traumatic reticuloperitonitis was recorded after the start of treatment. Based on the results of clinical, ultrasonographic and radiographic examinations, the cows were divided into two groups: Group A consisted of 12 cows without an intramural or perforating foreign body and group B had 10 cows with an intramural or perforating foreign body. Cows of group A received amoxicillin, flunixin meglumine and a NaCl-glucose infusion, and cows of group B received the same treatment accompanied by foreign body removal via ruminotomy. All cows were fitted with a horse halter equipped with a pressure sensor integrated into the noseband, and eating and rumination activities were recorded continuously for 7 days. Additional 24-hour- recording periods occurred on days 14 and 30. Results obtained previously from 300 healthy cows were used for comparison. Median daily eating time in group A was 168 minutes (reference interval 211 to 319 min) on day 1 and increased to within the reference interval by day 3. Eating time on day 14 was 290 minutes and significantly longer than on day 1. Eating time was 294 minutes on day 30. Eating time in group B was comparable, and the patterns of normalisation for the number of chewing cycles related to eating, rumination time, the number of regurgitated cuds per day and the number of chewing cycles per cud were similar to that of group A: all variables were significantly reduced on day 1 and normalised within 3 to 5 days. The findings showed that eating and rumination are reduced in cows with traumatic reticuloperitonitis and that successful treatment results in rapid normalisation. Monitoring eating and rumination behaviour using a pressure sensor integrated into a halter allows objective clinical assessment of cows recovering from traumatic reticuloperitonitis.

  10. 2. VIEW LOOKING NORTHWEST AT CHINA WASH FLUME ON MAIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW LOOKING NORTHWEST AT CHINA WASH FLUME ON MAIN CANAL - San Carlos Irrigation Project, China Wash Flume, Main (Florence-Case Grande) Canal at Station 137+00, T4S, R10E, S14, Coolidge, Pinal County, AZ

  11. 1. VIEW LOOKING WEST AT CHINA WASH FLUME ON MAIN ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW LOOKING WEST AT CHINA WASH FLUME ON MAIN CANAL. - San Carlos Irrigation Project, China Wash Flume, Main (Florence-Case Grande) Canal at Station 137+00, T4S, R10E, S14, Coolidge, Pinal County, AZ

  12. Speciation of mercury in sludge solids: washed sludge

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bannochie, C. J.; Lourie, A. P.

    2017-10-24

    The objective of this applied research task was to study the type and concentration of mercury compounds found within the contaminated Savannah River Site Liquid Waste System (SRS LWS). A method of selective sequential extraction (SSE), developed by Eurofins Frontier Global Sciences1,2 and adapted by SRNL, utilizes an extraction procedure divided into seven separate tests for different species of mercury. In the SRNL’s modified procedure four of these tests were applied to a washed sample of high level radioactive waste sludge.

  13. Patterns of stillbirth and dystocia in Ontario cow-calf herds.

    PubMed Central

    McDermott, J J; Allen, O B; Martin, S W; Alves, D M

    1992-01-01

    The association between a number of individual animal and herd level factors and calving problems in beef cows and heifers were examined. Data were from the 1987 calving season for a subset of 123 herds which maintained individual-animal records, from a sample of 180 randomly selected Ontario cow-calf herds. The median herd dystocia rate was 5.8% and 24.4% of herds had no dystocias. The median herd stillbirth rate was 2.8%, and 33.3% of herds had no stillbirths. Dystocias and stillbirths were much more common in heifers than in cows. Separate statistical models of dystocia and stillbirth for cows and heifers were created. Dystocia in cows was associated with calf sex, previous calving assistance and large breed type and birth weight. Variations in 1987 cow herd dystocia rates were associated with calving season, location and density, and the herd dystocia rate in 1986. Dystocia in heifers was associated with large breed type and calf birth weight. Herd-level management practices associated with increased heifer dystocia rates included breeding heifers to calve earlier than cows and rearing heifers together with the cow herd. Stillbirths for both cows and heifers were associated with calving assistance, particularly hard assistance. Herd-level management and other factors were unassociated with stillbirths. PMID:1586893

  14. A Window-Washing Challenge

    ERIC Educational Resources Information Center

    Roman, Harry T.

    2010-01-01

    Skyscrapers sure do have a lot of windows, and these windows are cleaned and checked regularly. All this takes time, money, and puts workers at potential risk. Might there be a better way to do it? In this article, the author discusses a window-washing challenge and describes how students can tackle this task, pick up the challenge, and creatively…

  15. Crossbreeding: implications for dairy cow fertility and survival.

    PubMed

    Buckley, F; Lopez-Villalobos, N; Heins, B J

    2014-05-01

    In pasture-based seasonal calving systems, failure to become pregnant during the breeding season results in important economic losses as maximum profit is attained by minimising costs and increasing the proportion of grass in the diet of the lactating dairy cow. In the United States, dairy producers primarily strive to maximise production potential but are becoming increasingly aware of the economic consequences of sub-optimal cow fertility and survival. For this reason, interest in crossbreeding is emerging. The objective of this paper is to review the fertility and survival outcomes reported from recent research studies and data analyses in Ireland, New Zealand and the United States. Research conducted in Ireland during the early 2000s concluded that of three 'alternative' dairy breeds the Norwegian Red was most suited to seasonal grass-based production. A key finding was favourable fertility and survival. A follow-up study confirmed a fertility advantage with Norwegian Red×Holstein-Friesian compared with Holstein-Friesian: proportion pregnant to first service; +0.08 and in-calf after 6 weeks breeding; +0.11. Another study found higher fertility with Jersey crossbreds: pregnant to first service; +0.21, and in-calf after 6 weeks breeding; +0.19. Studies conducted in Northern Ireland also found superior fertility performance with Jersey crossbred cows offered low and moderate concentrate diets. In New Zealand, crossbred dairy cattle (primarily Jersey×Friesian) are achieving similar rates of genetic gain for farm profit as the purebred populations, but creating additional gain derived from economic heterosis. In the United States, analysis of commercial data from California showed higher first-service conception rates for Scandinavian Red×Holstein (+6 percentage units) and Montbeliarde×Holstein (+10 percentage units) compared with Holstein (23%). They also exhibited fewer days open and greater survival. At Penn State University, Brown Swiss×Holstein cows had 17

  16. Effect of water washing on the thermal behavior of rice straw.

    PubMed

    Said, N; Bishara, T; García-Maraver, A; Zamorano, M

    2013-11-01

    Rice straw can be used as a renewable fuel for heat and power generation. It is a viable mean of replacing fossil fuels and preventing pollution caused by open burning, especially in the areas where this residual biomass is generated. Nevertheless, the thermal conversion of rice straw can cause some operating problems such as slag formation, which negatively affects thermal conversion systems. So, the main objective of this research is studying the combustion behavior of rice straw samples collected from various regions by applying thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). In addition, the thermal behavior of ashes from rice straw was also analyzed in order to detect their melting points, and ash sintering was detected at different temperatures within the range between 550 and 1000°C. Since washing rice straw with water could reduce the content of undesirable inorganic compounds related to the ash fusibility, samples of washed rice straw were analyzed under combustion conditions to investigate its differences regarding the thermal behavior of rice straw. The results showed that rice straw washing led to a significant improvement in its thermal behavior, since it reduced the ash contents and sintering formation. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. Multilocation trial of ceftiofur for treatment of postpartum cows with fever.

    PubMed

    Zhou, C; Boucher, J F; Dame, K J; Moreira, M; Graham, R; Nantel, J; Zuidhof, S; Arfi, L; Flores, R; Neubauer, G; Olson, J

    2001-09-15

    To evaluate the effect of ceftiofur for treatment of postpartum cows with fever. Multilocation randomized complete block design trial. 330 cows. Cows with rectal temperature > or = 39.5 C (103.1 F) during the first 10 postpartum days were randomly assigned to a treatment (ceftiofur; 1 mg/kg [0.45 mg/lb] of body weight daily for 3 days) or untreated control group. Cure (no additional or alternative antimicrobial treatment used, rectal temperature < 39.5 C, and no other concurrent clinical signs of disease when evaluated at 9 or 10 days after enrollment), milk production, and rectal temperature were evaluated. Ceftiofur-treated cows were significantly more likely to be cured than control cows (56.0 vs 28.9%, respectively), with an odds ratio of 3.14 when vaginal discharge (a factor with moderate interaction with treatment) was present at enrollment. Among cows that had an abnormal calving (a significant interaction factor), treated cows had first milking yield 2.27 kg (5 lb) greater than control cows. Treated cows had a significantly greater reduction in rectal temperature (1.19 C [2.14 Fl), compared with control cows (1.04 C [1.87 F]). Parenteral administration of ceftiofur significantly improved cure rate, milk yield, and rectal temperature in postpartum cows with fever and vaginal discharge or dystocia. These findings provide information to determine appropriate treatment for postpartum cows, which for years has been debated in the dairy industry.

  18. BLAISDELL SLOW SAND FILTER WASHING MACHINE. VIEW LOOKING WEST. THE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    BLAISDELL SLOW SAND FILTER WASHING MACHINE. VIEW LOOKING WEST. THE NONHISTORIC CHEMICAL BUILDING IS SEEN IN THE BACKGROUND. - Yuma Main Street Water Treatment Plant, Blaisdell Slow Sand Filter Washing Machine, Jones Street at foot of Main Street, Yuma, Yuma County, AZ

  19. Cow bones char as a green sorbent for fluorides removal from aqueous solutions: batch and fixed-bed studies.

    PubMed

    Nigri, Elbert M; Cechinel, Maria Alice P; Mayer, Diego A; Mazur, Luciana P; Loureiro, José M; Rocha, Sônia D F; Vilar, Vítor J P

    2017-01-01

    Cow bone char was investigated as sorbent for the defluoridation of aqueous solutions. The cow bone char was characterized in terms of its morphology, chemical composition, and functional groups present on the bone char surface using different analytical techniques: SEM, EDS, N 2 -BET method, and FTIR. Batch equilibrium studies were performed for the bone chars prepared using different procedures. The highest sorption capacities for fluoride were obtained for the acid washed (q = 6.2 ± 0.5 mg/g) and Al-doped (q = 6.4 ± 0.3 mg/g) bone chars. Langmuir and Freundlich models fitted well the equilibrium sorption data. Fluoride removal rate in batch system is fast in the first 5 h, decreasing after this time until achieving equilibrium due to pore diffusion. The presence of carbonate and bicarbonate ions in the aqueous solution contributes to a decrease of the fluoride sorption capacity of the bone char by 79 and 31 %, respectively. Regeneration of the F-loaded bone char using 0.5 M NaOH solution leads to a sorption capacity for fluoride of 3.1 mg/g in the second loading cycle. Fluoride breakthrough curve obtained in a fixed-bed column presents an asymmetrical S-shaped form, with a slow approach of C/C 0  → 1.0 due to pore diffusion phenomena. Considering the guideline value for drinking water of 1.5 mg F - /L, as recommended by World Health Organization, the service cycle for fluoride removal was of 71.0 h ([F - ] feed  ∼ 9 mg/L; flow rate = 1 mL/min; m sorbent  = 12.6 g). A mass transfer model considering the pore diffusion was able to satisfactorily describe the experimental data obtained in batch and continuous systems.

  20. Inflammatory biomarkers are associated with ketosis in periparturient Holstein cows.

    PubMed

    Abuajamieh, Mohannad; Kvidera, Sara K; Fernandez, Maria V Sanz; Nayeri, Amir; Upah, Nathan C; Nolan, Erin A; Lei, Sam M; DeFrain, Jeffery M; Green, Howard B; Schoenberg, Katie M; Trout, William E; Baumgard, Lance H

    2016-12-01

    Ketosis is a prevalent periparturient metabolic disorder and we hypothesize that lipopolysaccharide (LPS) infiltration may play a key role in its etiology. Study objectives were to characterize biomarkers of inflammation during the transition period in healthy and clinically diagnosed ketotic cows. Cows were retrospectively categorized into one of two groups: healthy and clinically diagnosed ketotic. Two data sets were utilized; the first dataset (Study A) was obtained as a subset of cows (n=16) enrolled in a larger experiment conducted at the Iowa State University Dairy utilizing Holstein cows (8 healthy; 8 ketotic), and the second dataset (Study B; 22 healthy; 22 ketotic) was obtained from a commercial farm. For both experiments, blood samples were collected prior to and following calving. Ketotic cows in both studies had reduced milk production compared to healthy cows (P<0.01). Post-calving, ketotic cows had increased serum amyloid A (4.2 and 1.8 fold in studies A and B, respectively; P=0.03 and P=0.04), haptoglobin (>6 fold and ~4 fold; P=0.04 and P=0.03), and lipopolysaccharide binding protein (66 and 45%; P<0.01 and P=0.02) compared with their healthy counterparts. Antepartum circulating LPS in ketotic cows was increased (2.3 fold; P=0.01) compared to healthy cows in Study B. In summary, increased biomarkers of inflammation appear to be closely associated with ketosis in transition dairy cows. Copyright © 2016 Elsevier Ltd. All rights reserved.

  1. Genetic relationships between feed efficiency in growing males and beef cow performance.

    PubMed

    Crowley, J J; Evans, R D; Mc Hugh, N; Kenny, D A; McGee, M; Crews, D H; Berry, D P

    2011-11-01

    Most studies on feed efficiency in beef cattle have focused on performance in young animals despite the contribution of the cow herd to overall profitability of beef production systems. The objective of this study was to quantify, using a large data set, the genetic covariances between feed efficiency in growing animals measured in a performance-test station, and beef cow performance including fertility, survival, calving traits, BW, maternal weaning weight, cow price, and cull cow carcass characteristics in commercial herds. Feed efficiency data were available on 2,605 purebred bulls from 1 test station. Records on cow performance were available on up to 94,936 crossbred beef cows. Genetic covariances were estimated using animal and animal-dam linear mixed models. Results showed that selection for feed efficiency, defined as feed conversion ratio (FCR) or residual BW gain (RG), improved maternal weaning weight as evidenced by the respective genetic correlations of -0.61 and 0.57. Despite residual feed intake (RFI) being phenotypically independent of BW, a negative genetic correlation existed between RFI and cow BW (-0.23; although the SE of 0.31 was large). None of the feed efficiency traits were correlated with fertility, calving difficulty, or perinatal mortality. However, genetic correlations estimated between age at first calving and FCR (-0.55 ± 0.14), Kleiber ratio (0.33 ± 0.15), RFI (-0.29 ± 0.14), residual BW gain (0.36 ± 0.15), and relative growth rate (0.37 ± 0.15) all suggest that selection for improved efficiency may delay the age at first calving, and we speculate, using information from other studies, that this may be due to a delay in the onset of puberty. Results from this study, based on the estimated genetic correlations, suggest that selection for improved feed efficiency will have no deleterious effect on cow performance traits with the exception of delaying the age at first calving.

  2. Is cow's milk harmful to a child's health?

    PubMed

    Agostoni, Carlo; Turck, Dominique

    2011-12-01

    Discussions and debates have recently emerged on the potential positive and negative effects of cow's milk in the paediatric community, also under the pressure of public opinion. The negative effects of cow's-milk consumption seem to be limited to iron status up to 9 to 12 months; then no negative effects are observed, provided that cow's milk, up to a maximum daily intake of 500 mL, is adequately complemented with iron-enriched foods. Lactose intolerance can be easily managed and up to 250 mL/day of milk can be consumed. Allergy to cow's-milk proteins is usually transient. Atopic children may independently be at risk for poor growth, and the contribution of dairy nutrients to their diet should be considered. The connection of cow's milk to autistic spectrum disorders is lacking, and even a cause-effect relation with type 1 diabetes mellitus has not been established because many factors may concur. Although it is true that cow's milk stimulates insulin-like growth factor-1 and may affect linear growth, association with chronic degenerative, noncommunicable diseases has not been established. Finally, fat-reduced milk, if needed, should be considered after 24 to 36 months. Cow's milk represents a major source of high nutritional quality protein as well as of calcium. Moreover, it has growth-promoting effects independent of specific compounds. Its protein and fat composition, together with the micronutrient content, is suggestive of a functional food, whose positive effects are emphasised by regular consumption, particularly under conditions of diets poor in some limiting nutrients, although in industrialised countries cow's milk's optimal daily intake should be around 500 mL, adequately complemented with other relevant nutrients.

  3. Replacing wheat with canola meal and maize grain in the diet of lactating dairy cows: Feed intake, milk production and cow condition responses.

    PubMed

    McDonnell, Ruairi P; Staines, Martin vH

    2017-08-01

    This research paper describes the effect of partially replacing wheat with maize grain and canola meal on milk production and body condition changes in early lactation Holstein-Friesian dairy cows consuming a grass silage-based diet over an 83-d period. Two groups of 39 cows were stratified for age, parity, historical milk yield and days in milk (DIM), and offered one of two treatment diets. The first treatment (CON) reflected a typical diet used by Western Australian dairy producers in summer and comprised (kg DM/cow per d); 8 kg of annual ryegrass silage, 6 kg of crushed wheat (provided once daily in a mixed ration), 3·6 kg of crushed lupins (provided in the milking parlour in two daily portions) and ad libitum lucerne haylage. The second treatment diet (COMP) was identical except the 6 kg of crushed wheat was replaced by 6 kg of a more complex concentrate mix (27% crushed wheat, 34% maize grain and 37% canola meal). Lucerne haylage was provided independently in the paddock to all cows, and no pasture was available throughout the experiment. The COMP group had a greater mean overall daily intake (22·5 vs 20·4 kg DM/cow) and a higher energy corrected milk (ECM) yield (29·2 vs 27·1 kg/cow; P = 0·047) than the CON cows. The difference in overall intake was caused by a higher daily intake of lucerne haylage in COMP cows (4·5 vs 2·3 kg DM/cow). The CON group had a higher concentration of milk fat (42·1 vs 39·3 g/kg; P = 0·029) than COMP cows. Milk protein yield was greater in COMP cows (P < 0·021); however, milk fat yield was unaffected by treatment. It is concluded that partially replacing wheat with canola meal and maize grain in a grass silage-based diet increases voluntary DMI of conserved forage and consequently yields of ECM and milk protein.

  4. Contrasting effects of progesterone on fertility of dairy and beef cows.

    PubMed

    Stevenson, J S; Lamb, G C

    2016-07-01

    The role of progesterone in maintaining pregnancy is well known in the bovine. Subtle differences exist between dairy and beef cows because of differing concentrations of progesterone during recrudescence of postpartum estrous cycles, rate of follicular growth and maturation, proportions of 2- and 3-follicular wave cycles, and other effects on pregnancy outcomes per artificial insemination (P/AI). Because proportions of anovulatory cows before the onset of the artificial insemination (AI) period are greater and more variable in beef (usually ranging from 30 to 70%) than dairy (25%) cows, AI programs were developed to accommodate anovulatory and cycling beef cows enrolled therein. Incorporating a progestin as part of an AI program in beef cows improved P/AI by reducing the proportion of cows having premature luteal regression and short post-AI luteal phases. In both genotypes, prolonged dominant follicle growth in a reduced progesterone milieu resulted in increased (1) LH pulses, (2) preovulatory follicle diameter, and (3) concentrations of estradiol and a subsequently larger corpora lutea (CL). In contrast, the progesterone milieu during growth of the ovulatory follicle in an ovulation control program does not seem to affect subsequent P/AI in beef cows, whereas in dairy cows follicle development in an elevated compared with a low progesterone environment increases P/AI. Progesterone status in beef cows at the onset of ovulation synchronization is not related to P/AI in multiparous cows, whereas P/AI was suppressed in primiparous cows that began a timed AI program in a low-progesterone environment. In timed AI programs, elevated concentrations of progesterone just before PGF2α and reduced concentrations at AI are critical to maximizing subsequent P/AI in dairy cows, but seemingly much less important in beef cows. By inducing ancillary CL and increasing concentrations of progesterone, human chorionic gonadotropin may increase P/AI when administered to beef cows 7d

  5. Invited review: Current perspectives on eating and rumination activity in dairy cows.

    PubMed

    Beauchemin, K A

    2018-06-01

    Many early studies laid the foundation for our understanding of the mechanics of chewing, the physiological role of chewing for the cow, and how chewing behavior is affected by dietary characteristics. However, the dairy cow has changed significantly over the past decades, as have the types of diets fed and the production systems used. The plethora of literature published in recent years provides new insights on eating and ruminating activity of dairy cows. Lactating dairy cows spend about 4.5 h/d eating (range: 2.4-8.5 h/d) and 7 h/d ruminating (range: 2.5-10.5 h/d), with a maximum total chewing time of 16 h/d. Chewing time is affected by many factors, most importantly whether access to feed is restricted, intake of neutral detergent fiber from forages, and mean particle size of the diet. Feed restriction and long particles (≥19 mm) have a greater effect on eating time, whereas intake of forage neutral detergent fiber and medium particles (4-19 mm) affects rumination time. It is well entrenched in the literature that promoting chewing increases salivary secretion of dairy cows, which helps reduce the risk of acidosis. However, the net effect of a change in chewing time on rumen buffing is likely rather small; therefore, acidosis prevention strategies need to be broad. Damage to plant tissues during mastication creates sites that provide access to fungi, adhesion of bacteria, and formation of biofilms that progressively degrade carbohydrates. Rumination and eating are the main ways in which feed is reduced in particle size. Contractions of the rumen increase during eating and ruminating activity and help move small particles to the escapable pool and into the omasum. Use of recently developed low-cost sensors that monitor chewing activity of dairy cows in commercial facilities can provide information that is helpful in management decisions, especially when combined with other criteria. Although accuracy and precision can be somewhat variable depending on sensor

  6. Omasal sampling technique for assessing fermentative digestion in the forestomach of dairy cows.

    PubMed

    Huhtanen, P; Brotz, P G; Satter, L D

    1997-05-01

    A procedure allowing digesta sampling from the omasum via a ruminal cannula without repeated entry into the omasum was developed. The sampling system consisted of a device inserted into the omasum via the ruminal cannula, a tube connecting the device to the ruminal cannula, and a single compressor/vacuum pump. Eight cows given ad libitum access to a total mixed diet were used in a crossover design to evaluate the effects of the sampling system on digestive activity, animal performance, and animal behavior. Results indicated that the omasal sampling system has minimal effect on normal digestive and productive functions of high-producing dairy cows. Dry matter intake was reduced (24.0 vs 21.8 kg/d; P < .02) and seemed related more to the sampling procedures than to the device in the omasum. Observations of animal behavior indicated that cows with the sampling device were similar to control cows, although rumination and total chewing times were reduced slightly. The composition of digesta samples was biased toward an over-abundance of the liquid phase, but using a double-marker system to calculate digesta flow resulted in fairly small coefficients of variation for measurements of ruminal digestion variables. This technique may prove useful for partitioning digestion between the fermentative portion of the forestomach and the lower gastrointestinal tract. The omasal sampling procedure requires less surgical intervention than the traditional methods using abomasal or duodenal cannulas as sampling sites to study forestomach digestion and avoids potentially confounding endogenous secretions of the abomasum.

  7. Can washing-pretreatment eliminate the health risk of municipal solid waste incineration fly ash reuse?

    PubMed

    Wang, Yao; Pan, Yun; Zhang, Lingen; Yue, Yang; Zhou, Jizhi; Xu, Yunfeng; Qian, Guangren

    2015-01-01

    Although the reuse of washing-pretreated MSWI fly ash bas been a hot topic, the associated risk is still an issue of great concern. The present study investigated the influence of washing-pretreatment on the total contents and bioaccessibility of heavy metals in MSWI fly ash. Furthermore, the study incorporated bioaccessibility adjustment into probabilistic risk assessment, to quantify the health risk from multi-pathway exposure to the concerned chemicals as a result of reusing washed MSWI fly ash. The results revealed that both water-washing and acid-washing process have resulted in the concentrated heavy metal content, and have reduced the bioaccessibility of heavy metals. Besides, the acid-washing process increased the cancer risk in most cases, while the effect of water-washing process was uncertain. However, both water-washing and acid-washing pretreatment could decrease the hazard index based on bioaccesilbility. Despite the uncertainties accompanying these procedures, the results indicated that, in this application scenario, only water-washing or acid-washing process cannot reduce the actual risk from all samples to acceptable level, especially for cancer risk. Copyright © 2014. Published by Elsevier Inc.

  8. Immunodiagnostic identification of dairy cows infected with Prototheca zopfii at various clinical stages and discrimination between infected and uninfected cows.

    PubMed

    Roesler, U; Scholz, H; Hensel, A

    2001-02-01

    Protothecosis is a severe form of mastitis in cattle that is caused by colorless algae of the genus Prototheca. So far, no suitable serological test for the identification of infected animals is available for routine diagnosis. In this study an indirect enzyme-linked immunosorbent assay (ELISA) for the identification of infected cows and for discriminating among infected cows at various clinical stages was developed. Immunoglobulin G (IgG) in serum and IgA and IgG1 in whey were used as antibody isotypes. The ELISA was evaluated using serum and whey from animals at different clinical stages of infection. A total of 12 cows with acute clinical manifestation of protothecal mastitis, 22 cows with clinical signs of chronic mastitis, 40 Prototheca zopfii-negative cows, and 18 cows with chronic clinical signs and earlier cultures positive for P. zopfii but with presently negative culturing results were investigated. A sensitivity of 96% and a specificity of 94% were calculated for the ELISA based on IgA levels. Intra-assay and interassay variations were calculated to be 6.08 and 6.32%, respectively. Based on these data, this ELISA was found to be suitable for discrimination between infected and uninfected animals and might therefore be useful for screening affected herds.

  9. Immunodiagnostic Identification of Dairy Cows Infected with Prototheca zopfii at Various Clinical Stages and Discrimination between Infected and Uninfected Cows

    PubMed Central

    Roesler, Uwe; Scholz, Holger; Hensel, Andreas

    2001-01-01

    Protothecosis is a severe form of mastitis in cattle that is caused by colorless algae of the genus Prototheca. So far, no suitable serological test for the identification of infected animals is available for routine diagnosis. In this study an indirect enzyme-linked immunosorbent assay (ELISA) for the identification of infected cows and for discriminating among infected cows at various clinical stages was developed. Immunoglobulin G (IgG) in serum and IgA and IgG1 in whey were used as antibody isotypes. The ELISA was evaluated using serum and whey from animals at different clinical stages of infection. A total of 12 cows with acute clinical manifestation of protothecal mastitis, 22 cows with clinical signs of chronic mastitis, 40 Prototheca zopfii-negative cows, and 18 cows with chronic clinical signs and earlier cultures positive for P. zopfii but with presently negative culturing results were investigated. A sensitivity of 96% and a specificity of 94% were calculated for the ELISA based on IgA levels. Intra-assay and interassay variations were calculated to be 6.08 and 6.32%, respectively. Based on these data, this ELISA was found to be suitable for discrimination between infected and uninfected animals and might therefore be useful for screening affected herds. PMID:11158103

  10. Fertility, survival, and conformation of Montbéliarde × Holstein and Viking Red × Holstein crossbred cows compared with pure Holstein cows during first lactation in 8 commercial dairy herds.

    PubMed

    Hazel, A R; Heins, B J; Hansen, L B

    2017-11-01

    Montbéliarde (MO) × Holstein (HO) and Viking Red (VR) × HO crossbred cows were compared with pure HO cows in 8 large, high-performance dairy herds in Minnesota. All cows calved for the first time from December 2010 to April 2014. Fertility and survival traits were calculated from records of insemination, pregnancy diagnosis, calving, and disposal that were recorded via management software. Body condition score and conformation were subjectively scored once during early lactation by trained evaluators. The analysis of survival to 60 d in milk included 536 MO × HO, 560 VR × HO, and 1,033 HO cows during first lactation. Cows analyzed for other fertility, survival, and conformation traits had up to 13% fewer cows available for analysis. The first service conception rate of the crossbred cows (both types combined) increased 7%, as did the conception rate across the first 5 inseminations, compared with the HO cows during first lactation. Furthermore, the combined crossbred cows (2.11 ± 0.05) had fewer times bred than HO cows (2.30 ± 0.05) and 10 fewer d open compared with their HO herdmates. Across the 8 herds, breed groups did not differ for survival to 60 d in milk; however, the superior fertility of the crossbred cows allowed an increased proportion of the combined crossbreds (71 ± 1.5%) to calve a second time within 14 mo compared with the HO cows (63 ± 1.5%). For survival to second calving, the combined crossbred cows had 4% superior survival compared with the HO cows. The MO × HO and VR × HO crossbred cows both had increased body condition score (+0.50 ± 0.02 and +0.25 ± 0.02, respectively) but shorter stature and less body depth than HO cows. The MO × HO cows had less set to the hock and a steeper foot angle than the HO cows, and the VR × HO cows had more set to the hock with a similar foot angle to the HO cows. The combined crossbred cows had less udder clearance from the hock than HO cows, more width between both front and rear teats, and longer

  11. Hand Washing Among School Children in Bogotá, Colombia

    PubMed Central

    Lopez-Quintero, Catalina; Freeman, Paul

    2009-01-01

    Objectives. We assessed hand-washing behaviors and intentions among school children in Bogotá, Colombia, to help identify and overcome barriers to proper hygiene practices. Methods. Data on hand-washing behavior and intentions and individual and contextual factors were collected from 2042 sixth- through eighth-grade students in 25 schools in Bogotá via anonymous questionnaires. A member of the school administration or teaching staff completed a questionnaire about the school environment. Site inspections of bathroom facilities were conducted. Results. Only 33.6% of the sample reported always or very often washing hands with soap and clean water before eating and after using the toilet. About 7% of students reported regular access to soap and clean water at school. A high level of perceived control was the strongest predictor of positive hand-washing intentions (adjusted odds ratio [AOR] = 6.0; 95% confidence interval [CI] = 4.8, 7.5). Students with proper hand-washing behavior were less likely to report previous-month gastrointestinal symptoms (OR = 0.8; 95% CI = 0.6, 0.9) or previous-year school absenteeism (OR = 0.7; 95% CI = 0.6, 0.9). Conclusions. Scarcity of adequate facilities in most schools in Bogotá prevents children from adopting proper hygienic behavior and thwarts health promotion efforts. The current renovation program of public schools in Bogotá provides a unique opportunity to meet the challenges of providing a supportive environment for adoption of healthy behaviors. PMID:19008513

  12. Wash room, bunkhouse, first floor interior. This room is a ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Wash room, bunkhouse, first floor interior. This room is a screened porch with the original sinks extant. Light and ventilation was borrowed from the wash room into the toilets and bathing rooms. - Sespe Ranch, Bunkhouse, 2896 Telegraph Road, Fillmore, Ventura County, CA

  13. Fatal winter dysentery with severe anemia in an adult cow.

    PubMed

    Natsuaki, Sumiko; Goto, Keiichi; Nakamura, Kikuyasu; Yamada, Manabu; Ueo, Hiroshi; Komori, Toshihiro; Shirakawa, Hitomi; Uchinuno, Yukinori

    2007-09-01

    An adult dairy cow fatally affected with winter dysentery was investigated pathologically and virologically. The cow had severe anemia and diarrhea with massive blood. Pathologically, the loss of surface epithelial cells and necrosis of crypt epithelial cells in the large intestine were observed. Bovine coronavirus (BCV) antigen was observed in necrotic crypt epithelial cells of the large intestine. Virus particles were found in the necrotic epithelial cells of the large intestine. Virologically, BCV was isolated from the feces of the dead cow. The dead cow had no serum antibody against BCV although the co-habitants did. These suggest that severe infection of BCV in the cow without the BCV antibody accompanied by severe hemorrhagic anemia resulted in the cow's death.

  14. Evaluation of microplastic release caused by textile washing processes of synthetic fabrics.

    PubMed

    De Falco, Francesca; Gullo, Maria Pia; Gentile, Gennaro; Di Pace, Emilia; Cocca, Mariacristina; Gelabert, Laura; Brouta-Agnésa, Marolda; Rovira, Angels; Escudero, Rosa; Villalba, Raquel; Mossotti, Raffaella; Montarsolo, Alessio; Gavignano, Sara; Tonin, Claudio; Avella, Maurizio

    2018-05-01

    A new and more alarming source of marine contamination has been recently identified in micro and nanosized plastic fragments. Microplastics are difficult to see with the naked eye and to biodegrade in marine environment, representing a problem since they can be ingested by plankton or other marine organisms, potentially entering the food web. An important source of microplastics appears to be through sewage contaminated by synthetic fibres from washing clothes. Since this phenomenon still lacks of a comprehensive analysis, the objective of this contribution was to investigate the role of washing processes of synthetic textiles on microplastic release. In particular, an analytical protocol was set up, based on the filtration of the washing water of synthetic fabrics and on the analysis of the filters by scanning electron microscopy. The quantification of the microfibre shedding from three different synthetic fabric types, woven polyester, knitted polyester, and woven polypropylene, during washing trials simulating domestic conditions, was achieved and statistically analysed. The highest release of microplastics was recorded for the wash of woven polyester and this phenomenon was correlated to the fabric characteristics. Moreover, the extent of microfibre release from woven polyester fabrics due to different detergents, washing parameters and industrial washes was evaluated. The number of microfibres released from a typical 5 kg wash load of polyester fabrics was estimated to be over 6,000,000 depending on the type of detergent used. The usage of a softener during washes reduces the number of microfibres released of more than 35%. The amount and size of the released microfibres confirm that they could not be totally retained by wastewater treatments plants, and potentially affect the aquatic environment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. A tradeoff study of determine the optimum approach to a wash/rinse capability to support future space flight

    NASA Technical Reports Server (NTRS)

    Wilson, D. A.

    1976-01-01

    Specific requirements for a wash/rinse capability to support Spacelab biological experimentation and to identify various concepts for achieving this capability were determined. This included the examination of current state-of-the-art and emerging technology designs that would meet the wash/rinse requirements. Once several concepts were identified, including the disposable utensils, tools and gloves or other possible alternatives, a tradeoff analysis involving system cost, weight, volume utilization, functional performance, maintainability, reliability, power utilization, safety, complexity, etc., was performed so as to determine an optimum approach for achieving a wash/rinse capability to support future space flights. Missions of varying crew size and durations were considered.

  16. Evaluation of different water-washing treatments effects on wheat straw combustion properties.

    PubMed

    Ma, Qiulin; Han, Lujia; Huang, Guangqun

    2017-12-01

    A series of experiments was conducted to explore the effects of various water-washing solid-liquid ratios (1:50 and 1:10) and the stirring on wheat straw (WS) combustion properties. Comparing different solid-liquid ratio groups, a 16% increment in the higher heating value was obtained for 1:50 groups and only 5% for 1:10 groups relative to the raw material. Moreover, energy was lost 4-26 times greater in 1:10 groups than 1:50 groups. While water-washing reduced the comprehensive combustibility index by 14.89%-32.09%, the index values of washed WS were all higher than 2, indicating good combustion performance. The combustion activation energy of four washed WS were 175, 172, 186, and 176kJ/mol, which were all higher than the 160kJ/mol of WS. The fouling/slagging propensity of washed WS reduced to a lower possibility compared to medium of untreated WS. Overall, the recommended condition for washing WS before combustion is 1:50 ratio without stirring. Copyright © 2017. Published by Elsevier Ltd.

  17. e-Cow: an animal model that predicts herbage intake, milk yield and live weight change in dairy cows grazing temperate pastures, with and without supplementary feeding.

    PubMed

    Baudracco, J; Lopez-Villalobos, N; Holmes, C W; Comeron, E A; Macdonald, K A; Barry, T N; Friggens, N C

    2012-06-01

    This animal simulation model, named e-Cow, represents a single dairy cow at grazing. The model integrates algorithms from three previously published models: a model that predicts herbage dry matter (DM) intake by grazing dairy cows, a mammary gland model that predicts potential milk yield and a body lipid model that predicts genetically driven live weight (LW) and body condition score (BCS). Both nutritional and genetic drives are accounted for in the prediction of energy intake and its partitioning. The main inputs are herbage allowance (HA; kg DM offered/cow per day), metabolisable energy and NDF concentrations in herbage and supplements, supplements offered (kg DM/cow per day), type of pasture (ryegrass or lucerne), days in milk, days pregnant, lactation number, BCS and LW at calving, breed or strain of cow and genetic merit, that is, potential yields of milk, fat and protein. Separate equations are used to predict herbage intake, depending on the cutting heights at which HA is expressed. The e-Cow model is written in Visual Basic programming language within Microsoft Excel®. The model predicts whole-lactation performance of dairy cows on a daily basis, and the main outputs are the daily and annual DM intake, milk yield and changes in BCS and LW. In the e-Cow model, neither herbage DM intake nor milk yield or LW change are needed as inputs; instead, they are predicted by the e-Cow model. The e-Cow model was validated against experimental data for Holstein-Friesian cows with both North American (NA) and New Zealand (NZ) genetics grazing ryegrass-based pastures, with or without supplementary feeding and for three complete lactations, divided into weekly periods. The model was able to predict animal performance with satisfactory accuracy, with concordance correlation coefficients of 0.81, 0.76 and 0.62 for herbage DM intake, milk yield and LW change, respectively. Simulations performed with the model showed that it is sensitive to genotype by feeding environment

  18. Arsenic exposure to dairy cows in Bangladesh.

    PubMed

    Ghosh, Amalendu; Majumder, Shankar; Awal, Md Abdul; Rao, D Ramkishan

    2013-01-01

    Food-chain contamination by arsenic (As) is a newly uncovered disaster. Effects of As-contaminated drinking water and paddy straw on the excretion of As through milk, urine, and dung of dairy cows (n = 240) were studied in As-prone areas of Bangladesh. Mean (±SEM) total As (inorganic plus organic) concentration in drinking water, paddy straw [dry weight dw)], cow's urine (specific gravity adjusted to 1.035), dung (dw), and milk (wet weight) were 89.6 ± 6.5 μg/l, 1,114.4 ± 57.3 μg/kg, 123.6 ± 7.6 μg/l, 1,693.0 ± 65.1 μg/kg, and 26.2 ± 2.8 μg/l, respectively. Significantly (p < 0.01) greater As was in Boro straw (1,386.9 ± 71.8 μg/kg) than Aus (702.4 ± 67.1 μg/kg) and Aman (431.7 ± 28.8 μg/kg) straw and in straw irrigated with shallow (1,697.3 ± 81.9 μg/kg) than deep well water (583.6 ± 62.7 μg/kg) and surface water (511.8 ± 30.0 μg/kg). Significant (p < 0.01) positive correlations were found between As contents of cow's urine and drinking water (r = 0.92) as well as cow dung and straw (r = 0.82). Concentrations of As in cow urine, dung, and milk were increased with the relative increment of As in drinking water and/or straw. These results provide evidence that dairy cows excrete ingested As mainly through urine and dung; thus, As biotransformation through milk remains low. This low concentration of As in milk may be of concern when humans are exposed to multiple sources of As simultaneously. Moreover, As in cow dung could be an environmental issue in Bangladesh.

  19. Association between Prepartum Feeding Behavior and Periparturient Health Disorders in Dairy Cows

    PubMed Central

    Luchterhand, Karen M.; Silva, Paula R. B.; Chebel, Ricardo C.; Endres, Marcia I.

    2016-01-01

    The objective of this study was to investigate the relationship between prepartum feeding behavior, measured as time spent feeding per day, and periparturient health disorders, milk yield, milk composition, and milk somatic cell count in Jersey cows. Pregnant Jersey cows were marked with unique alphanumeric symbols and were moved into a prepartum group 4 weeks prior to their expected calving date. At enrollment, cows with a body condition score <2 or >4 or a locomotion score >3 were not included. Time spent feeding was measured using 10-min video scan sampling for 24-h periods of 2–4 days per week of the study. A total of 925 cows were eligible for analysis. Parity was based on lactation number at the time of enrollment and classified as nulliparous (cows pregnant with their first calf), primiparous (cows pregnant with their second calf), and multiparous (lactation ≥2). Multiparous cows with two or more health disorders spent approximately 10% less time feeding prepartum than cows that did not have any health disorders. Multiparous cows subsequently diagnosed with metritis had a tendency to spend 5% less time feeding prepartum than healthy counterparts. Primiparous cows with retained placenta had a 10% reduction in feeding time compared to healthy primiparous cows. Monitoring time spent feeding prepartum by primiparous and multiparous cows, even on a limited number of days, appeared to be beneficial in predicting cows at risk for periparturient health disorders. Real-time daily feeding behavior monitoring technologies that can be used by dairy farms are now available, which might prove to be even more helpful in identifying cows at risk for periparturient cow health disorders as more data points can be recorded for each cow and compared to her own behavior or that of specific cohorts. PMID:27597948

  20. Association between Washing Residue on the Feet and Tinea Pedis in Diabetic Patients

    PubMed Central

    Takehara, Kimie; Amemiya, Ayumi; Mugita, Yuko; Tsunemi, Yuichiro; Seko, Yoko; Ohashi, Yumiko; Ueki, Kohjiro; Kadowaki, Takashi; Nagase, Takashi; Ikeda, Mari; Sanada, Hiromi

    2015-01-01

    Tinea pedis (TP) may lead to the development of foot ulcers in diabetic patients; thus, its prevention in diabetic patients is important. TP occurs after dermatophytes on the skin scales of TP patients attach to the feet. Therefore, it is necessary to remove the scales and dermatophytes, and this can be performed using various methods, including foot washing. This study aimed to objectively examine the association between the presence of TP and foot-washing habits. We included 33 diabetic patients, and, of these, 17 had TP. The presence of washing residue on the feet was determined by applying a fluorescent cream to the participants' feet, and images of the feet were captured under ultraviolet light before and after foot washing. Our results showed that diabetic patients with TP had higher levels of washing residue on their feet than those without TP. The importance of washing feet to prevent TP needs to be emphasized through educational programs for diabetic patients. Furthermore, the development of an effective foot-washing technique is essential. PMID:25793125

  1. SOIL-WASHING TECHNOLOGY AND PRACTICE

    EPA Science Inventory

    Soil washing in the United States has been studied and evaluated with increasing thoroughness during the last 15 to 20 years. It is now entering a phase of actual use and acceptance as its applicability and economics become clearer. This paper reviews the principles behind soil...

  2. The Influence of Climate, Soil and Pasture Type on Productivity and Greenhouse Gas Emissions Intensity of Modeled Beef Cow-Calf Grazing Systems in Southern Australia

    PubMed Central

    Bell, Matthew J.; Cullen, Brendan R.; Eckard, Richard J.

    2012-01-01

    Simple Summary Livestock production systems and the agricultural industries in general face challenges to meet the global demand for food, whilst also minimizing their environmental impact through the production of greenhouse gas (GHG) emissions. Livestock grazing systems in southern Australia are low input and reliant on pasture as a low-cost source of feed. The balance between productivity and GHG emission intensity of beef cow-calf grazing systems was studied at sites chosen to represent a range of climatic zones, soil and pasture types. While the climatic and edaphic characteristics of a location may impact on the emissions from a grazing system, management to efficiently use pasture can reduce emissions per unit product. Abstract A biophysical whole farm system model was used to simulate the interaction between the historical climate, soil and pasture type at sites in southern Australia and assess the balance between productivity and greenhouse gas emissions (expressed in carbon dioxide equivalents, CO2-eq.) intensity of beef cow-calf grazing systems. Four sites were chosen to represent a range of climatic zones, soil and pasture types. Poorer feed quality and supply limited the annual carrying capacity of the kikuyu pasture compared to phalaris pastures, with an average long-term carrying capacity across sites estimated to be 0.6 to 0.9 cows/ha. A relative reduction in level of feed intake to productivity of calf live weight/ha at weaning by feeding supplementary feed reduced the average CO2-eq. emissions/kg calf live weight at weaning of cows on the kikuyu pasture (18.4 and 18.9 kg/kg with and without supplementation, respectively), whereas at the other sites studied an increase in intake level to productivity and emission intensity was seen (between 10.4 to 12.5 kg/kg without and with supplementary feed, respectively). Enteric fermentationand nitrous oxide emissions from denitrification were the main sources of annual variability in emissions intensity

  3. Reduced lymphoid response to skin allotransplants in cows with hepatic lipidosis.

    PubMed

    Wentink, G H; van den Ingh, T S; Rutten, V P; Müller, K E; Wensing, T

    1999-04-01

    The immune responsiveness of cows with hepatic lipidosis (fatty liver) in comparison to control cows with a normal liver fat content was tested by applying skin allotransplants to the skin of the back of cows on day 3 after parturition. Immunoreactivity was determined by semiquantitative counting of the number of infiltrating lymphocytes in the recipient skin adjacent to the allotransplants during a period of 21 days. There were more invading lymphocytes in samples from control cows than there were in samples from cows with hepatic lipidosis. It was concluded that cows with hepatic lipidosis have a reduced lymphoid response to skin allotransplants.

  4. Washing the guilt away: effects of personal versus vicarious cleansing on guilty feelings and prosocial behavior

    PubMed Central

    Xu, Hanyi; Bègue, Laurent; Bushman, Brad J.

    2014-01-01

    For centuries people have washed away their guilt by washing their hands. Do people need to wash their own hands, or is it enough to watch other people wash their hands? To induce guilt, we had participants write about a past wrong they had committed. Next, they washed their hands, watched a washing-hands video, or watched a typing-hands video. After the study was over, participants could help a Ph.D. student complete her dissertation by taking some questionnaires home and returning them within 3 weeks. Results showed that guilt and helping behavior were lowest among participants who washed their hands, followed by participants who watched a washing-hands video, followed by participants who watched a typing-hands video. Guilt mediated the effects of cleansing on helping. These findings suggest that washing one’s own hands, or even watching someone else wash their hands, can wash away one’s guilt and lead to less helpful behavior. PMID:24616686

  5. Increasing Hand Washing Compliance With a Simple Visual Cue

    PubMed Central

    Boyer, Brian T.; Menachemi, Nir; Huerta, Timothy R.

    2014-01-01

    We tested the efficacy of a simple, visual cue to increase hand washing with soap and water. Automated towel dispensers in 8 public bathrooms were set to present a towel either with or without activation by users. We set the 2 modes to operate alternately for 10 weeks. Wireless sensors were used to record entry into bathrooms. Towel and soap consumption rates were checked weekly. There were 97 351 hand-washing opportunities across all restrooms. Towel use was 22.6% higher (P = .05) and soap use was 13.3% higher (P = .003) when the dispenser presented the towel without user activation than when activation was required. Results showed that a visual cue can increase hand-washing compliance in public facilities. PMID:24228670

  6. Cow biological type affects ground beef colour stability.

    PubMed

    Raines, Christopher R; Hunt, Melvin C; Unruh, John A

    2009-12-01

    To determine the effects of cow biological type on colour stability of ground beef, M. semimembranosus from beef-type (BSM) and dairy-type (DSM) cows was obtained 5d postmortem. Three blends (100% BSM, 50% BSM+50% DSM, 100% DSM) were adjusted to 90% and 80% lean points using either young beef trim (YBT) or beef cow trim (BCT), then packaged in high oxygen (High-O(2); 80% O(2)) modified atmosphere (MAP). The BSM+YBT patties had the brightest colour initially, but discoloured rapidly. Although DSM+BCT patties had the darkest colour initially, they discoloured least during display. Metmyoglobin reducing ability of ground DSM was up to fivefold greater than ground BSM, and TBARS values of BSM was twofold greater than DSM by the end of display (4d). Though initially darker than beef cow lean, dairy cow lean has a longer display colour life and may be advantageous to retailers using High-O(2) MAP.

  7. Effects of egg shell quality and washing on Salmonella Infantis penetration.

    PubMed

    Samiullah; Chousalkar, K K; Roberts, J R; Sexton, M; May, D; Kiermeier, A

    2013-07-15

    The vast majority of eggs in Australia are washed prior to packing to remove dirt and fecal material and to reduce the microbial contamination of the egg shell. The egg contents can be an ideal growth medium for microorganisms which can result in human illness if eggs are stored improperly and eaten raw or undercooked, and it is estimated that egg-related salmonellosis is costing Australia $44 million per year. Egg shell characteristics such as shell thickness, amount of cuticle present, and thickness of individual egg shell layers can affect the ease with which bacteria can penetrate the egg shell and washing could partially or completely remove the cuticle layer. The current study was conducted to investigate the effects of egg washing on cuticle cover and effects of egg shell quality and cuticle cover on Salmonella Infantis penetration of the egg shell. A higher incidence of unfavorable ultrastructural variables of the mammillary layer such as late fusion, type B bodies, type A bodies, poor cap quality, alignment, depression, erosion and cubics were recorded in Salmonella penetrated areas of egg shells. The influence of egg washing on the ability of Salmonella Infantis on the egg shell surface to enter the egg internal contents was also investigated using culture-based agar egg penetration and real-time qPCR based experiments. The results from the current study indicate that washing affected cuticle cover. There were no significant differences in Salmonella Infantis penetration of washed or unwashed eggs. Egg shell translucency may have effects on Salmonella Infantis penetration of the egg shell. The qPCR assay was more sensitive for detection of Salmonella Infantis from egg shell wash and internal contents than traditional microbiological methods. The agar egg and whole egg inoculation experiments indicated that Salmonella Infantis penetrated the egg shells. Egg washing not only can be highly effective at removing Salmonella Infantis from the egg shell surface

  8. Monitoring Method of Cow Anthrax Based on Gis and Spatial Statistical Analysis

    NASA Astrophysics Data System (ADS)

    Li, Lin; Yang, Yong; Wang, Hongbin; Dong, Jing; Zhao, Yujun; He, Jianbin; Fan, Honggang

    Geographic information system (GIS) is a computer application system, which possesses the ability of manipulating spatial information and has been used in many fields related with the spatial information management. Many methods and models have been established for analyzing animal diseases distribution models and temporal-spatial transmission models. Great benefits have been gained from the application of GIS in animal disease epidemiology. GIS is now a very important tool in animal disease epidemiological research. Spatial analysis function of GIS can be widened and strengthened by using spatial statistical analysis, allowing for the deeper exploration, analysis, manipulation and interpretation of spatial pattern and spatial correlation of the animal disease. In this paper, we analyzed the cow anthrax spatial distribution characteristics in the target district A (due to the secret of epidemic data we call it district A) based on the established GIS of the cow anthrax in this district in combination of spatial statistical analysis and GIS. The Cow anthrax is biogeochemical disease, and its geographical distribution is related closely to the environmental factors of habitats and has some spatial characteristics, and therefore the correct analysis of the spatial distribution of anthrax cow for monitoring and the prevention and control of anthrax has a very important role. However, the application of classic statistical methods in some areas is very difficult because of the pastoral nomadic context. The high mobility of livestock and the lack of enough suitable sampling for the some of the difficulties in monitoring currently make it nearly impossible to apply rigorous random sampling methods. It is thus necessary to develop an alternative sampling method, which could overcome the lack of sampling and meet the requirements for randomness. The GIS computer application software ArcGIS9.1 was used to overcome the lack of data of sampling sites.Using ArcGIS 9.1 and GEODA

  9. Factors influencing hand washing behaviour in primary schools: process evaluation within a randomised controlled trial

    PubMed Central

    Chittleborough, Catherine R.; Nicholson, Alexandra L.; Basker, Elaine; Bell, Sarah; Campbell, Rona

    2013-01-01

    This paper explores factors that may influence hand washing behaviour among pupils and staff in primary schools. A qualitative process evaluation within a cluster randomised controlled trial included pupil focus groups (n=16, ages 6 to 11, semi-structured interviews (n=16 teachers) and observations of hand washing facilities (n=57). Pupils and staff in intervention and control schools demonstrated a similar level of understanding of how, when and why they should wash their hands. Lack of time, poor adult modelling of regular hand washing and unattractive facilities were seen as important barriers to regular hand washing. Reminders and explanations for the importance of hand hygiene were thought to have a positive impact. Influencing individual choices about hand washing through education and information may be necessary, but not sufficient, for initiating and maintaining good hand washing practices. Structural factors, including having time to wash hands using accessible, clean facilities, and being encouraged through the existence of hand washing opportunities in the daily routine and hand washing being viewed as the social norm, will also influence hand washing behaviour. The effectiveness of educational interventions at improving hand hygiene in primary schools may be improved by changing priorities of staff and increasing accessibility to quality facilities. PMID:22623617

  10. Factors influencing hand washing behaviour in primary schools: process evaluation within a randomized controlled trial.

    PubMed

    Chittleborough, Catherine R; Nicholson, Alexandra L; Basker, Elaine; Bell, Sarah; Campbell, Rona

    2012-12-01

    This article explores factors that may influence hand washing behaviour among pupils and staff in primary schools. A qualitative process evaluation within a cluster randomized controlled trial included pupil focus groups (n = 16, aged 6-11 years), semi-structured interviews (n = 16 teachers) and observations of hand washing facilities (n = 57). Pupils and staff in intervention and control schools demonstrated a similar level of understanding of how, when and why they should wash their hands. Lack of time, poor adult modelling of regular hand washing and unattractive facilities were seen as important barriers to regular hand washing. Reminders and explanations for the importance of hand hygiene were thought to have a positive impact. Influencing individual choices about hand washing through education and information may be necessary, but not sufficient, for initiating and maintaining good hand washing practices. Structural factors, including having time to wash hands using accessible, clean facilities, and being encouraged through the existence of hand washing opportunities in the daily routine and hand washing being viewed as the social norm, will also influence hand washing behaviour. The effectiveness of educational interventions at improving hand hygiene in primary schools may be improved by changing priorities of staff and increasing accessibility to quality facilities.

  11. Elevated Apoptosis in the Liver of Dairy Cows with Ketosis.

    PubMed

    Du, Xiliang; Chen, Liang; Huang, Dan; Peng, Zhicheng; Zhao, Chenxu; Zhang, Yuming; Zhu, Yiwei; Wang, Zhe; Li, Xinwei; Liu, Guowen

    2017-01-01

    Dairy cows with ketosis are characterized by oxidative stress and hepatic damage. The aim of this study was to investigate hepatic oxidative stress and the apoptotic status of ketotic cows, as well as the underlying apoptosis pathway. The blood aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamate dehydrogenase (GLDH) and gamma-glutamyl transferase (GGT) activities and the haptoglobin (HP), serum amyloid A (SAA) and serum apoptotic cytokeratin 18 neo-epitope M30 (CK18 M30) concentrations were determined by commercially available kits and ELISA kits, respectively. Liver histology, TUNEL and Oil red O staining were performed in liver tissue samples. TG contents were measured using an enzymatic kit; Caspase 3 assays were carried out using the Caspase 3 activity assay kit; oxidation and antioxidant markers were measured using biochemical kits; apoptosis pathway were determined by qRT-PCR and western blot. Ketotic cows displayed hepatic fat accumulation. The hepatic malondialdehyde (MDA) content was significantly increased, but the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were markedly decreased in ketotic cows compared with control cows, indicating that ketotic cows displayed severe oxidative stress. Significantly higher serum levels of the hepatic damage markers AST, ALT, GGT and GLDH were observed in ketotic cows than in control cows. The blood concentration of the apoptotic marker CK18 M30 and the number of TUNEL-positive cells in the liver of ketotic cows were 1.19- and 2.61-fold, respectively, higher than the values observed in control cows. Besides, Caspase 3 activity was significantly increased in the liver of ketosis cows. Importantly, the levels of phosphorylated c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38MAPK) were significantly increased but the level of phosphorylated extracellular signal-regulated kinase1/2 (ERK1/2) was markedly decreased, which

  12. Randomized noninferiority clinical trial evaluating 3 commercial dry cow mastitis preparations: II. Cow health and performance in early lactation.

    PubMed

    Arruda, A G; Godden, S; Rapnicki, P; Gorden, P; Timms, L; Aly, S S; Lehenbauer, T W; Champagne, J

    2013-10-01

    The objective of this randomized noninferiority clinical trial was to compare the effect of treatment with 3 different dry cow therapy formulations at dry-off on cow-level health and production parameters in the first 100 d in milk (DIM) in the subsequent lactation, including 305-d mature-equivalent (305 ME) milk production, linear score (LS), risk for the cow experiencing a clinical mastitis event, risk for culling or death, and risk for pregnancy by 100 DIM. A total of 1,091 cows from 6 commercial dairy herds in 4 states (California, Iowa, Minnesota, and Wisconsin) were randomly assigned at dry-off to receive treatment with 1 of 3 commercial products: Quartermaster (QT; Zoetis Animal Health, Madison, NJ), Spectramast DC (SP; Zoetis Animal Health) or ToMorrow Dry Cow (TM; Boehringer Ingelheim Vetmedica Inc., St Joseph, MO). All clinical mastitis, pregnancy, culling, and death events occurring in the first 100 DIM were recorded by farm staff using an on-farm electronic record-keeping system. Dairy Herd Improvement Association test-day records of milk production and milk component testing were retrieved electronically. Mixed linear regression analysis was used to describe the effect of treatment on 305ME milk production and LS recorded on the last Dairy Herd Improvement Association test day before 100 DIM. Cox proportional hazards regression analysis was used to describe the effect of treatment on risk for experiencing a case of clinical mastitis, risk for leaving the herd, and risk for pregnancy between calving and 100 DIM. Results showed no effect of treatment on adjusted mean 305 ME milk production (QT=11,759 kg, SP=11,574 kg, and TM=11,761 kg) or adjusted mean LS (QT=1.8, SP=1.9, and TM=1.6) on the last test day before 100 DIM. Similarly, no effect of treatment was observed on risk for a clinical mastitis event (QT=14.8%, SP=12.7%, and TM=15.0%), risk for leaving the herd (QT=7.5%, SP=9.2%, and TM=10.3%), or risk for pregnancy (QT=31.5%, SP=26.1%, and TM=26

  13. Decision tree analysis to evaluate dry cow strategies under UK conditions.

    PubMed

    Berry, Elizabeth A; Hogeveen, Henk; Hillerton, J Eric

    2004-11-01

    Economic decisions on animal health strategies address the cost-benefit aspect along with animal welfare and public health concerns. Decision tree analysis at an individual cow level highlighted that there is little economic difference between the use of either dry cow antibiotic or an internal teat sealant in preventing a new intramammary infection in a cow free of infection in all quarters of the mammary gland at drying off. However, a potential net loss of over ł20 per cow might occur if the uninfected cow was left untreated. The only economically viable option, for a cow with one or more quarters infected at drying off, is antibiotic treatment, although a loss might still be incurred depending on the pathogen concerned and the cure rates achievable. There was a net loss for cows with quarters infected with Corynebacterium spp. at drying off, for both the teat sealant and untreated groups (ł22 and ł48, respectively) with only antibiotic-treated cows showing a gain.

  14. Effects of supplementing methionine hydroxy analog on beef cow performance, milk production, reproduction, and preweaning calf performance.

    PubMed

    Clements, A R; Ireland, F A; Freitas, T; Tucker, H; Shike, D W

    2017-12-01

    Mature Simmental × Angus cows (214 cows; 635 ± 7 kg) were utilized to determine the effects of late gestation and early postpartum supplementation of methionine hydroxy analog (MHA) on cow BW, BCS, milk production, milk composition, reproduction, and calf performance until weaning in a fall-calving, cool-season grazing system. Cows were stratified by BW, age, AI sire, and assigned to 1 of 12 pastures (17 or 18 cows·pasture). Pastures were randomly allotted to 1 of 2 treatments: control (0.45 kg·cow·d of wheat midd-based pellets, = 6) or supplement including MHA (0.45 kg·cow·d of wheat midd-based pellets including 10 g MHA supplied as MFP (Novus International, Inc., St. Charles, MO; = 6). Treatments were fed 23 ± 7 d prepartum through 73 ± 7 d postpartum. Cow BW was collected at postcalving (27 ± 7 d postpartum), end of supplementation (73 ± 7 d postpartum), AI, pregnancy check, and end of trial (192 and 193 ± 7 d postpartum). At 73 ± 7 d postpartum, a subset of cow-calf pairs was used in a weigh-suckle-weigh procedure to determine milk production, and milk samples were taken to determine milk composition ( = 45·treatment). Serum from blood was collected at 73 ± 7 and 83 ± 7 d postpartum to determine cow cyclicity and concentrations of 2-hydroxy4-(methylthio) butanoic acid (HMTBa) and L-Methionine. After supplementation, all cow-calf pairs were managed as a common group until weaning (193 ± 7 d of age). Cows were bred via AI at 97 ± 7 d postpartum and clean-up bulls were turned out 11 d post-AI for a 55-d breeding season. Cows fed MHA had greater ( < 0.01) serum concentrations of HMTBa. Cow BW and BCS were not different ( ≥ 0.10) at any time points between treatments. There was no treatment effect ( ≥ 0.17) on calf birth BW, calf weaning BW (193 ± 7 d of age), or calf ADG. Calculated 24-h milk production, milk composition and component production did not differ ( ≥ 0.21). There were no differences ( ≥ 0.50) in percentage of cows cycling

  15. Comparison of Fecal Microbiota between German Holstein Dairy Cows with and without Left-Sided Displacement of the Abomasum

    PubMed Central

    Song, Eun-Sik; Jung, Sang Il; Park, Hyung-Jin; Seo, Kyoung-Won; Son, Jeong-Hoon; Hong, Sanghyun; Shim, Minkyung

    2016-01-01

    One of the most common diseases in high-performance German Holstein dairy cows is left-sided displacement of the abomasum (LDA). Hypomotility of the abomasum is detrimental during the pathogenesis of LDA. It is known that improper interactions between the gut microbiota and the enteric nervous system contribute to dysfunctions of gastrointestinal motility. Therefore, we hypothesized that the gut microbial composition will be different between German Holstein dairy cows with and without LDA. We used 16S rRNA gene analysis to evaluate whether there are any differences in bacterial composition between German Holstein dairy cows with and without LDA. Even though our data are limited to being used to correlate compositional changes with corresponding functional aspects in the pathogenesis of LDA, results from this study show that the fecal microbial compositions of German Holstein dairy cows with LDA shifted and were less diverse than those in normal cows. In particular, Spirochaetes were absent in cows with LDA. PMID:26842700

  16. Effects of β-hydroxybutyrate and isoproterenol on lipolysis in isolated adipocytes from periparturient dairy cows and cows with clinical ketosis.

    PubMed

    van der Drift, S G A; Everts, R R; Houweling, M; van Leengoed, L A M G; Stegeman, J A; Tielens, A G M; Jorritsma, R

    2013-06-01

    An in vitro model was used to investigate effects of β-hydroxybutyrate and isoproterenol (β-adrenergic receptor agonist) on lipolysis in isolated adipocytes from late pregnant and recently calved dairy cows (n=5) and cows with clinical ketosis (n=3). Incubation with 3.0 mmol/L β-hydroxybutyrate reduced lipolysis in isolated adipocytes. This inhibitory effect was lower in the first lactation week (47%±16%) compared with late pregnancy (71%±6.5%). Incubation with 0.3 μmol/L isoproterenol stimulated lipolysis in isolated adipocytes from periparturient dairy cows. Basal lipolysis resulted in non-esterified fatty acid to glycerol ratios in the incubation media of 2.0±0.23 in prepartum samples, 2.1±0.23 in the first lactation week and 2.2±0.09 in cows with clinical ketosis. β-Hydroxybutyrate reduced lipolysis by 45%±9.6% in isolated adipocytes from cows with clinical ketosis, indicating that impaired feedback of β-hydroxybutyrate may not play a role in the disease etiology. Copyright © 2012 Elsevier Ltd. All rights reserved.

  17. Eddy covariance methane flux measurements over a grazed pasture: effect of cows as moving point sources

    NASA Astrophysics Data System (ADS)

    Felber, R.; Münger, A.; Neftel, A.; Ammann, C.

    2015-06-01

    Methane (CH4) from ruminants contributes one-third of global agricultural greenhouse gas emissions. Eddy covariance (EC) technique has been extensively used at various flux sites to investigate carbon dioxide exchange of ecosystems. Since the development of fast CH4 analyzers, the instrumentation at many flux sites has been amended for these gases. However, the application of EC over pastures is challenging due to the spatially and temporally uneven distribution of CH4 point sources induced by the grazing animals. We applied EC measurements during one grazing season over a pasture with 20 dairy cows (mean milk yield: 22.7 kg d-1) managed in a rotational grazing system. Individual cow positions were recorded by GPS trackers to attribute fluxes to animal emissions using a footprint model. Methane fluxes with cows in the footprint were up to 2 orders of magnitude higher than ecosystem fluxes without cows. Mean cow emissions of 423 ± 24 g CH4 head-1 d-1 (best estimate from this study) correspond well to animal respiration chamber measurements reported in the literature. However, a systematic effect of the distance between source and EC tower on cow emissions was found, which is attributed to the analytical footprint model used. We show that the EC method allows one to determine CH4 emissions of cows on a pasture if the data evaluation is adjusted for this purpose and if some cow distribution information is available.

  18. Eddy covariance methane flux measurements over a grazed pasture: effect of cows as moving point sources

    NASA Astrophysics Data System (ADS)

    Felber, R.; Münger, A.; Neftel, A.; Ammann, C.

    2015-02-01

    Methane (CH4) from ruminants contributes one third to global agricultural greenhouse gas emissions. Eddy covariance (EC) technique has been extensively used at various flux sites to investigate carbon dioxide exchange of ecosystems. Since the development of fast CH4 analysers the instrumentation at many flux sites have been amended for these gases. However the application of EC over pastures is challenging due to the spatial and temporal uneven distribution of CH4 point sources induced by the grazing animals. We applied EC measurements during one grazing season over a pasture with 20 dairy cows (mean milk yield: 22.7 kg d-1) managed in a rotational grazing system. Individual cow positions were recorded by GPS trackers to attribute fluxes to animal emissions using a footprint model. Methane fluxes with cows in the footprint were up to two orders of magnitude higher than ecosystem fluxes without cows. Mean cow emissions of 423 ± 24 g CH4 head-1 d-1 (best guess of this study) correspond well to animal respiration chamber measurements reported in the literature. However a systematic effect of the distance between source and EC tower on cow emissions was found which is attributed to the analytical footprint model used. We show that the EC method allows to determine CH4 emissions of grazing cows if the data evaluation is adjusted for this purpose and if some cow distribution information is available.

  19. Metabolic Heat Stress Adaption in Transition Cows: Differences in Macronutrient Oxidation between Late-Gestating and Early-Lactating German Holstein Dairy Cows

    PubMed Central

    Derno, Michael; Otten, Winfried; Mielenz, Manfred; Nürnberg, Gerd

    2015-01-01

    High ambient temperatures have severe adverse effects on biological functions of high-yielding dairy cows. The metabolic adaption to heat stress was examined in 14 German Holsteins transition cows assigned to two groups, one heat-stressed (HS) and one pair-fed (PF) at the level of HS. After 6 days of thermoneutrality and ad libitum feeding (P1), cows were challenged for 6 days (P2) by heat stress (temperature humidity index (THI) = 76) or thermoneutral pair-feeding in climatic chambers 3 weeks ante partum and again 3 weeks post-partum. On the sixth day of each period P1 or P2, oxidative metabolism was analyzed for 24 hours in open circuit respiration chambers. Water and feed intake, vital parameters and milk yield were recorded. Daily blood samples were analyzed for glucose, β-hydroxybutyric acid, non-esterified fatty acids, urea, creatinine, methyl histidine, adrenaline and noradrenaline. In general, heat stress caused marked effects on water homeorhesis with impairments of renal function and a strong adrenergic response accompanied with a prevalence of carbohydrate oxidation over fat catabolism. Heat-stressed cows extensively degraded tissue protein as reflected by the increase of plasma urea, creatinine and methyl histidine concentrations. However, the acute metabolic heat stress response in dry cows differed from early-lactating cows as the prepartal adipose tissue was not refractory to lipolytic, adrenergic stimuli, and the rate of amino acid oxidation was lower than in the postpartal stage. Together with the lower endogenous metabolic heat load, metabolic adaption in dry cows is indicative for a higher heat tolerance and the prioritization of the nutritional requirements of the fast-growing near-term fetus. These findings indicate that the development of future nutritional strategies for attenuating impairments of health and performance due to ambient heat requires the consideration of the physiological stage of dairy cows. PMID:25938406

  20. Metabolic Heat Stress Adaption in Transition Cows: Differences in Macronutrient Oxidation between Late-Gestating and Early-Lactating German Holstein Dairy Cows.

    PubMed

    Lamp, Ole; Derno, Michael; Otten, Winfried; Mielenz, Manfred; Nürnberg, Gerd; Kuhla, Björn

    2015-01-01

    High ambient temperatures have severe adverse effects on biological functions of high-yielding dairy cows. The metabolic adaption to heat stress was examined in 14 German Holsteins transition cows assigned to two groups, one heat-stressed (HS) and one pair-fed (PF) at the level of HS. After 6 days of thermoneutrality and ad libitum feeding (P1), cows were challenged for 6 days (P2) by heat stress (temperature humidity index (THI) = 76) or thermoneutral pair-feeding in climatic chambers 3 weeks ante partum and again 3 weeks post-partum. On the sixth day of each period P1 or P2, oxidative metabolism was analyzed for 24 hours in open circuit respiration chambers. Water and feed intake, vital parameters and milk yield were recorded. Daily blood samples were analyzed for glucose, β-hydroxybutyric acid, non-esterified fatty acids, urea, creatinine, methyl histidine, adrenaline and noradrenaline. In general, heat stress caused marked effects on water homeorhesis with impairments of renal function and a strong adrenergic response accompanied with a prevalence of carbohydrate oxidation over fat catabolism. Heat-stressed cows extensively degraded tissue protein as reflected by the increase of plasma urea, creatinine and methyl histidine concentrations. However, the acute metabolic heat stress response in dry cows differed from early-lactating cows as the prepartal adipose tissue was not refractory to lipolytic, adrenergic stimuli, and the rate of amino acid oxidation was lower than in the postpartal stage. Together with the lower endogenous metabolic heat load, metabolic adaption in dry cows is indicative for a higher heat tolerance and the prioritization of the nutritional requirements of the fast-growing near-term fetus. These findings indicate that the development of future nutritional strategies for attenuating impairments of health and performance due to ambient heat requires the consideration of the physiological stage of dairy cows.

  1. Geophysical Studies Based on Gravity and Seismic Data of Tule Desert, Meadow Valley Wash, and California Wash Basins, Southern Nevada

    USGS Publications Warehouse

    Scheirer, Daniel S.; Page, William R.; Miller, John J.

    2006-01-01

    Gravity and seismic data from Tule Desert, Meadow Valley Wash, and California Wash, Nevada, provide insight into the subsurface geometry of these three basins that lie adjacent to rapidly developing areas of Clark County, Nevada. Each of the basins is the product of Tertiary extension accommodated with the general form of north-south oriented, asymmetrically-faulted half-grabens. Geophysical inversion of gravity observations indicates that Tule Desert and Meadow Valley Wash basins are segmented into subbasins by shallow, buried basement highs. In this study, basement refers to pre-Cenozoic bedrock units that underlie basins filled with Cenozoic sedimentary and volcanic units. In Tule Desert, a small, buried basement high inferred from gravity data appears to be a horst whose placement is consistent with seismic reflection and magnetotelluric observations. Meadow Valley Wash consists of three subbasins separated by basement highs at structural zones that accommodated different styles of extension of the adjacent subbasins, an interpretation consistent with geologic mapping of fault traces oblique to the predominant north-south fault orientation of Tertiary extension in this area. California Wash is a single structural basin. The three seismic reflection lines analyzed in this study image the sedimentary basin fill, and they allow identification of faults that offset basin deposits and underlying basement. The degree of faulting and folding of the basin-fill deposits increases with depth. Pre-Cenozoic units are observed in some of the seismic reflection lines, but their reflections are generally of poor quality or are absent. Factors that degrade seismic reflector quality in this area are rough land topography due to erosion, deformed sedimentary units at the land surface, rock layers that dip out of the plane of the seismic profile, and the presence of volcanic units that obscure underlying reflectors. Geophysical methods illustrate that basin geometry is more

  2. Environmental Impacts Of Zirab Coal Washing Plant, Mazandaran, Iran

    NASA Astrophysics Data System (ADS)

    Moore, F.; Esmaeili, A.

    2009-04-01

    Extraction and beneficiation operations associated with coal mining increase the rate of chemical reaction of waste material to air and water media. Zirab coal washing plant is located on the bank of the Cherat stream in Mazandaran province, Iran. coal Mined from central Alborz coalfield mines is not suitable for use in Iranian Steel Corporation. Hence, coal ash content is reduced by physical and chemical processes in this plant. These processes leave a large quantity of liquid and solid wastes that accumulate in waste dump and tailing dam. sediment and water samples taken from Sheshrudbar and Cherat streams and also from Talar river show high concentration of Cd, Mo and As in water samples of coal washing plant and the associated drainage. Eh-pH diagrams revealed the chemical species of elements in water. The enrichment factor and geoaccumulation index show that Cd, Hg, Mo and V are enriched in bottom sediments of the coal washing plant and decrease with increasing distance from the plant. Sequential extraction analysis Results of three sediment samples of Cherat stream show that silicate bound is the major phase in samples taken before and after the plant, but adjacent to the plant, organic bound is dominant. The high concentration of Cd and Mo in the water soluble phase, is noticeable and may result in high mobility and bioavailability of these elements. Mann-Whitney and Wilcoxon tests on six samples, before and after the coal washing plant support the obtained results. Keywords: Zirab; coal washing plant; Sequential extraction analysis; Mann-whitney; Wilcoxon; Enrichment factor; Geoaccumulation index.

  3. Organochlorine pesticide distribution in an organic production system for cow's milk in Chiapas, Mexico.

    PubMed

    Murga, María N; Gutiérrez, Rey; Vega, Salvador; Pérez, José J; Ortiz, Rutilio; Schettino, Beatriz; Yamasaki, Alberto; Ruíz, Jorge L

    2016-09-01

    The objective of this study was to evaluate the presence of organochlorine pesticides in samples of forage, soil, water, and milk in four units of an organic production system for cow´s milk (samples of forage, milk, soil, and water) in Tecpatan, Chiapas, Mexico. The organochlorine pesticides were extracted from forage, soil and water based on the USEPA (2005) guideline and from milk based on the IDF 1991 guideline. The pesticides were identified and quantified by gas chromatography with electron capture detector (CG-ECD). In general, the highest average concentration of total pesticides was found in the samples of milk and forage (311 ± 328 and 116.5 ±77 ng g(-1) respectively). Although, the production systems analyzed are organic, organochlorine pesticides were detected in all environmental samples (forage, soil, water, and organic milk). Although no values surpassed the defined limits of Mexican and International regulation it is advisable that a monitoring program of contaminants in these production systems is continued.

  4. Concentrate Supplement Modifies the Feeding Behavior of Simmental Cows Grazing in Two High Mountain Pastures.

    PubMed

    Romanzin, Alberto; Corazzin, Mirco; Piasentier, Edi; Bovolenta, Stefano

    2018-05-16

    During grazing on Alpine pastures, the use of concentrates in dairy cows' diet leads to a reduction of the environmental sustainability of farms, and influences the selective pressure on some plant species. In order to minimize the use of concentrates, it is imperative to obtain data on the grazing behavior of cows. The aim of this study was to assess the effect of concentrate levels on the behavior of dairy cows during grazing. One hundred and ten lactating Italian Simmental cows, that sequentially grazed two pastures characterized by Poion alpinae (Poion) and Seslerion caeruleae (Seslerion) alliance, were considered. For each pasture, eight cows were selected and assigned to two groups: High and Low, supplemented with 4 kg/head/d, and 1 kg/head/d of concentrate respectively. Cows were equipped with a noseband pressure sensor and a pedometer (RumiWatch system, ITIN-HOCH GmbH) to assess grazing, ruminating, and walking behavior. In addition, the plant selection of the animals was assessed. On Poion, increased supplement intake caused a more intense selection of legumes, without affecting feeding and walking times. On Seslerion, grazing time was higher in Low than High. Grazing management in alpine region must take into account the great variability of pastures that largely differ from a floristic and nutritional point of view.

  5. A comparison of the effect of short-acting and long-acting cloxacillin-based dry-cow therapy on somatic cell counts after calving in cows also given internal teat sealants.

    PubMed

    Whitfield, L K; Laven, R A

    2018-01-01

    To compare, in cows treated with an internal teat sealant, the effect of short-acting and long-acting cloxacillin-based dry-cow therapy on somatic cell counts (SCC) after calving. Cows from a spring-calving, pasture-based dairy farm in the Manawatu-Whanganui region of New Zealand were randomly allocated to receive either a short-acting cloxacillin and ampicillin dry-cow therapy and internal teat sealant (n=291) or a long-acting cloxacillin and ampicillin dry-cow therapy and internal teat sealant (n=288) at the end of lactation. Cows were managed on-farm with routine husbandry procedures through the dry period and following calving. A multivariable logistic regression model was used to determine the association between length of action of dry-cow therapy and the proportion of cows with a SCC >150,000 cells/mL at the first herd test after calving. Age of cow, mean SCC for the preceding season and interval from calving to the first post-calving herd test were all associated with the proportion of cows with an individual SCC >150,000 cells/mL at the first herd test (p<0.001) Treatment with the short-acting dry-cow therapy was not associated with decreased odds of cows having a SCC >150,000 cells/mL at the first herd test compared with treatment with long-acting dry-cow therapy (OR=0.724; 95% CI=0.40-1.30). In this herd, which routinely used internal teat sealants, the use of short-acting cloxacillin-based dry-cow therapy did not result in an increased proportion of cows with elevated SSC post-calving. This was a single farm, single year study but indicates that in this herd, changing from a long-acting to a short-acting antimicrobial may have no impact on the prevalence of subclinical mastitis.

  6. Associations between cow hygiene, hock injuries, and free stall usage on US dairy farms.

    PubMed

    Lombard, J E; Tucker, C B; von Keyserlingk, M A G; Kopral, C A; Weary, D M

    2010-10-01

    This cross-sectional study evaluated cow comfort measures in free stall dairies across the United States as part of the National Animal Health Monitoring System's Dairy 2007 study. The study was conducted in 17 states and evaluations were completed between March 5 and September 5, 2007. Assessors recorded hygiene and hock scores, number of cows housed in the pen, the number of cows standing with only the front feet in a stall, standing fully in a stall, and lying in a stall. Facility design measures included bedding type, bedding quantity, stall length and width, presence of a neck rail or brisket locator, and relevant distances from the rear and bed of the stall. Of the 491 operations that completed the cow comfort assessment, 297 had Holstein cows housed in free stalls and were included in this analysis. Negative binomial models were constructed to evaluate the following outcomes: the number of cows that were very dirty, had severe hock injuries, stood with front feet in the stall, stood with all feet in the stall, and were lying in the stall. Hygiene was better on farms that did not tail dock cows compared with those that did (5.7 vs. 8.8% were dirty) and on farms located in the study's west region compared with those located in the east region (5.2 vs. 9.7% were dirty). Severe hock injuries were less common on farms in the west than those in the east (0.5 vs. 4.1%). In addition, severe hock injuries were less common on farms that used dirt as a stall base or sand as bedding compared with farms that did not. A higher percentage of cows was standing with front feet in the stall at higher ambient temperatures (incidence rate ratio=1.016) and as time since feeding increased (incidence rate ratio=1.030). A lower percentage of cows were standing with front feet in the stall when the stalls were shorter and when there were fewer cows per stall. Standing fully in a stall was performed by a higher percentage of cows during the summer than during the spring (13.6 vs. 8

  7. Effect of moving dairy cows at different stages of labor on behavior during parturition.

    PubMed

    Proudfoot, K L; Jensen, M B; Heegaard, P M H; von Keyserlingk, M A G

    2013-03-01

    Cows are often moved from a group to an individual maternity pen just before calving. However, it is unclear whether moving cows during labor may alter their behavior or affect the progress of labor. The aim of this study was to determine if moving cows to a maternity pen at different stages of labor would influence calving behavior or the length of the second stage of labor. Seventy-nine multiparous Holstein dairy cows were moved from 1 of 2 group pens to 1 of 10 maternity pens adjacent to each group pen either 3 d before expected calving date or when one or more behavioral or physical signs of labor were observed. These signs were noted, and were used to retrospectively categorize cows into 1 of 3 movement categories: (1) moved before labor, (2) moved during early stage I labor (signs of suddenly tense and enlarged udder, raised tail or relaxed pelvic ligaments; could also be immediately prelabor), or (3) moved during late stage I labor (signs of viscous, bloody mucus or abdominal contractions; could also be transitioning to stage II labor). Calves were weighed within 12h of birth and remained with their dam for 3 d. The length of the second stage of labor (the time between first abdominal contractions to the delivery the calf) and the total time of abdominal contractions, lying time, and number of position changes from standing to lying made by the cow in the hour before calving were recorded. A single blood sample was taken from the jugular vein of cows 3 to 27 h after calving to determine content of haptoglobin, a marker of systemic inflammation. The effect of movement category on length of the second stage of labor and behavioral variables was tested with ANOVA; category was a fixed effect and calf body weight (BW) and cow parity were covariates. The relationship between haptoglobin and the length of the second stage of labor was tested in a model with time of sampling relative to calving as a covariate. Cows moved during late stage I had the longest labor

  8. Dairy cow feeding space requirements assessed in a Y-maze choice test.

    PubMed

    Rioja-Lang, F C; Roberts, D J; Healy, S D; Lawrence, A B; Haskell, M J

    2012-07-01

    The effect of proximity to a dominant cow on a low-ranking cow's willingness to feed was assessed using choice tests. The main aim of the experiment was to determine the feeding space allowance at which the majority of subordinate cows would choose to feed on high-palatability food (HPF) next to a dominant cow rather than feeding alone on low-palatability food (LPF). Thirty Holstein-Friesian cows were used in the study. Half of the cows were trained to make an association between a black bin and HPF and a white bin and LPF, and the other half were trained with the opposite combination. Observations of pair-wise aggressive interactions were observed during feeding to determine the relative social status of each cow. From this, dominant and subordinate cows were allocated to experimental pairs. When cows had achieved an HPF preference with an 80% success rate in training, they were presented with choices using a Y-maze test apparatus, in which cows were offered choices between feeding on HPF with a dominant cow and feeding on LPF alone. Four different space allowances were tested at the HPF feeder: 0.3, 0.45, 0.6, and 0.75 m. At the 2 smaller space allowances, cows preferred to feed alone (choices between feeding alone or not for 0.3- and 0.45-m tests were significantly different). For the 2 larger space allowances, cows had no significant preferences (number of choices for feeding alone or with a dominant). Given that low-status cows are willing to sacrifice food quality to avoid close contact with a dominant animal, we suggest that the feeding space allowance should be at least 0.6m per cow whenever possible. However, even when space allowances are large, it is clear that some subordinate cows will still prefer to avoid proximity to dominant individuals. Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  9. [Prevalence and severity of lameness in early lactation in dairy cows and the effect on reproductive performance].

    PubMed

    Orgel, Corina; Ruddat, Inga; Hoedemaker, Martina

    2016-08-17

    To examine the impact of different degrees of lameness on the fertility of dairy cows. Seven dairy herds housed in free stall barns with cubicles were visited at 4-week intervals and all cows were examined for locomotion characteristics using a locomotion scoring system. The cows received scores from 1 (normal gait) to 5 (severely lame). During the study period of 18 months, 893 cows in the first month of lactation and 944 cows in the second, third and fourth months of lactation were evaluated. Reproductive measures between non-lame cows (score 1), mild lame cows (score 2) and cows with clear lameness (score ≥ 3) were compared. Independent of parity and herd effects, cows with scores ≥ 3 in the first month of lactation displayed a 6 days longer calving to first service interval (CFSI) (86.2 ± 35.6 vs. 80.2 ± 34.1 days; p = 0.002) and a 16 days longer calving to conception interval (CCI) (119.7 ± 51.4 vs. 103.8 ± 55.5 days; p = 0.048) than non-lame cows. Additionally, the risk for non-conception was significantly higher in cows suffering from lameness in the first month of lactation (odds ratio: 2.1, 95% confidence interval: 1.095, 4.040; p = 0.026). Cows affected by mild lameness in the third month of lactation had a 4 days longer CFSI (84.7 ± 35.6 vs. 80.1 ± 30.5 days; p = 0.026) and a 18 days longer CCI (120.1 ± 55.4 vs. 102.3 ± 47.6 days; p = 0.016) than non-lame cows, while the CSFI in distinct lame cows was extended by 7 days compared to non-lame cows (87.2 ± 34.9 vs. 80.1 ± 30.5 days; p = 0.002). Cows with the score 1 in the first month and score 3 in the third month of lactation showed the longest CSFI (94.3 ± 33.9 vs. 76.5 ± 16.0 days in non-lame cows; p = 0.046). This study suggests that both distinct and mild lameness in early lactation lead to a decline in reproduction in dairy herds. A regular determination of the lameness prevalence in dairy herds and optimizing claw health management

  10. The economic benefit of treating subclinical Streptococcus agalactiae mastitis in lactating cows.

    PubMed

    Yamagata, M; Goodger, W J; Weaver, L; Franti, C

    1987-12-15

    The economic benefits of treating lactating cows for Streptococcus agalactiae mastitis were studied at a large (689 milking cows) central California dairy. Postcure milk production of case cows (infected, treated, and cured) was compared with production of paired control cows (uninfected) and was matched for yield, days in milk, days in gestation, and parity. A simulation was used to plot expected lactation curves for mastitic cows (infected, not treated) with characteristics similar to those of each control cow, and these curves were compared with actual case-cow lactation curves. The difference in actual and expected production was used to calculate net economic benefits of treatment. Comparison of expected with actual production indicated a net benefit from treatment of $396/cow for cows treated in early lactation and $237 for cows treated in midlactation, but a net loss of $55 for cows treated in late lactation. Lactation number did not have a significant impact on economic benefits of treatment. In contrast to other studies indicating no economic benefit from treating mastitis during lactation, this study's positive results may have been attributable to the high cure rate (98%) and the subclinical form of mastitis being treated. Streptococcus agalactiae mastitis treatment during early and midlactation would appear to be an economically justifiable option for dairy managers.

  11. Short communication: Flooring preferences of dairy cows at calving.

    PubMed

    Campler, M; Munksgaard, L; Jensen, M B; Weary, D M; von Keyserlingk, M A G

    2014-02-01

    The present study investigated the flooring preference during the 30 h before parturition in Holstein dairy cows housed individually in a maternity pen. Seventeen multiparous cows were moved, on average, 2 d before expected calving date into an individual maternity pen with 3 different flooring surfaces: 10 cm of sand, pebble-top rubber mats, or concrete flooring, each covered with 15 cm of straw. Calving location, lying time, and total time and number of lying bouts on each of the floor types were recorded during 2 periods: precalving (24 to 29 h before calving) and at calving (0 to 5h before calving). Ten cows calved on sand, 6 on concrete, and 1 on the rubber mat. Lying bouts increased during the hours closest to calving, regardless of flooring. The number of lying bouts did not differ between flooring types precalving but cows had more lying bouts on sand and concrete compared with rubber at calving. Cows spent more time lying down on sand and concrete compared with rubber precalving, but lying times did not differ between treatments at calving. Cows that calved on sand spent more time lying on sand at calving compared with the other 2 flooring types. Cows that calved on concrete did not show a flooring preference at calving. These results indicate that rubber mats are the least preferred by dairy cows in the maternity pens, even when covered with a deep layer of straw. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  12. Bone metabolism in cow milk allergic children.

    PubMed

    Jakusova, Lubica; Jesenak, Milos; Schudichova, Jela; Banovcin, Peter

    2013-07-01

    Children with cow milk allergy are suspected to develop calcium metabolism disturbances. We observed increased markers of bone turnover in these children. Children with cow milk allergy are more prone to develop the disturbances of the bone mineralization even in the first year of life.

  13. 21 CFR 133.136 - Washed curd and soaked curd cheese.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Washed curd and soaked curd cheese. 133.136... (CONTINUED) FOOD FOR HUMAN CONSUMPTION CHEESES AND RELATED CHEESE PRODUCTS Requirements for Specific Standardized Cheese and Related Products § 133.136 Washed curd and soaked curd cheese. (a) Description. (1...

  14. 21 CFR 133.136 - Washed curd and soaked curd cheese.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Washed curd and soaked curd cheese. 133.136... (CONTINUED) FOOD FOR HUMAN CONSUMPTION CHEESES AND RELATED CHEESE PRODUCTS Requirements for Specific Standardized Cheese and Related Products § 133.136 Washed curd and soaked curd cheese. (a) Description. (1...

  15. 33 CFR 110.229 - Straits of Juan de Fuca, Wash.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Straits of Juan de Fuca, Wash. 110.229 Section 110.229 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.229 Straits of Juan de Fuca, Wash. (a) Anchorage...

  16. 33 CFR 110.229 - Straits of Juan de Fuca, Wash.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Straits of Juan de Fuca, Wash. 110.229 Section 110.229 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.229 Straits of Juan de Fuca, Wash. (a) Anchorage...

  17. Concentrations of buparvaquone in milk and tissue of dairy cows.

    PubMed

    McDougall, S; Hillerton, J E; Pegram, D

    2016-11-01

    To determine the concentration of the anti-theilerial drug buparvaquone in the milk and tissue of dairy cattle following treatment with two different formulations, and to assess the effect of clinical theileriosis on the concentration of buparvaquone in milk. Healthy lactating dairy cows (n=25) were injected once (Day 0) I/M with 2.5 mg/kg of one of two formulations of buparvaquone (Butalex; n=12 or Bupaject; n=13). Milk samples were collected from all cows daily until Day 35. Five cows were slaughtered on each of Days 56, 119, 147, 203 and 328, and samples of liver, muscle and injection site tissue collected. Milk samples were also collected from cows (n=14) clinically affected with theileriosis for up to 21 days after treatment with buparvaquone. Milk and tissue samples were analysed by liquid chromatography-mass spectrometry; limits of detection (LOD) were 0.00018 mg/kg for muscle and 0.00023 mg/L for milk. Concentrations of buparvaquone in milk and tissues were log10-transformed for analysis using multivariate models. In healthy cows, concentrations of buparvaquone in milk declined with time post-treatment (p<0.001), but were above the LOD in 11 of 25 cows at Day 35. Concentration in milk was higher one day after treatment in cows treated with Butalex than in cows treated with Bupaject, but not different thereafter (p=0.007). Concentrations of buparvaquone in muscle were below the LOD for four of five animals at Day 119 and for all animals by Day 147, but were above the LOD at the injection site of one cow, and in the liver of three cows at Day 328. Tissue concentrations did not differ with formulation nor was there a formulation by time interaction (p>0.3). Concentrations of buparvaquone in the milk of clinically affected animals were not different from those of healthy animals at 1 and 21 days post-treatment (p=0.72). Between 21 and 25 days post-treatment concentrations were below the LOD in 9/14 milk samples from clinically affected cows. Detectable

  18. Mad Cow Disease and U.S. Beef Trade

    DTIC Science & Technology

    2006-12-06

    1 For additional details and background see CRS Report RS22345, BSE (“Mad Cow Disease:): A Brief Overview, and CRS Report RL32199, Bovine Spongiform ...foreign markets that banned U.S. beef when a cow in Washington state tested positive for bovine spongiform encephalopathy (BSE, or mad cow disease) in...rejection of three shipments of U.S. beef because of the presence of bone fragments. CRS-4 8 See also CRS Report RL32932, Bovine Spongiform

  19. Treatment of tunnel wash water and implications for its disposal.

    PubMed

    Hallberg, M; Renman, G; Byman, L; Svenstam, G; Norling, M

    2014-01-01

    The use of road tunnels in urban areas creates water pollution problems, since the tunnels must be frequently cleaned for traffic safety reasons. The washing generates extensive volumes of highly polluted water, for example, more than fivefold higher concentrations of suspended solids compared to highway runoff. The pollutants in the wash water have an affinity for particulate material, so sedimentation should be a viable treatment option. In this study, 12 in situ sedimentation trials were carried out on tunnel wash water, with and without addition of chemical flocculent. Initial suspended solids concentration ranged from 804 to 9,690 mg/L. With sedimentation times of less than 24 hours and use of a chemical flocculent, it was possible to reach low concentrations of suspended solids (<15 mg/L), PAH (<0.1 μg/L), As (<1.0 μg/L), Cd (<0.05 μg/L), Hg (<0.02 μg/L), Fe (<200 μg/L), Ni (<8 μg/L), Pb (<0.5 μg/L), Zn (<60 μg/L) and Cr (<8 μg/L). Acute Microtox(®) toxicity, mainly attributed to detergents used for the tunnel wash, decreased significantly at low suspended solids concentrations after sedimentation using a flocculent. The tunnel wash water did not inhibit nitrification. The treated water should be suitable for discharge into recipient waters or a wastewater treatment plant.

  20. [Comparison of antibacterial effects of different antiseptics after hand washing].

    PubMed

    Buluş, Nesrin; Kaleli, Ilknur

    2004-01-01

    The aim of this study was to compare 4% chlorhexidine gluconate, 7.5% povidone iodine and liquid soap, which are used as hand washing solutions for the immediate, cumulative and residual effects in bacterial growth. For this purpose, 18 volunteers washed their hands with 7.5% povidone iodine, 4% chlorhexidine gluconate and liquid soap by applying standard hygienic hand washing technique. In order to find out the bacterial amount in the hands of study group, glove liquid test was used. For the evaluation of immediate effects, samples were collected just after the washing procedure, for the residual effects samples were collected from gloved hands after 3 hours, and for the cumulative effects, samples were collected after 5 days with daily hand washings. It is found that the immediate effects of 4% chlorhexidine gluconate was superior than others, and 7.5% povidone iodine was superior to liquid soap. There was no difference between 4% chlorhexidine gluconate and 7.5% povidone iodine for residual effects. The cumulative effects were observed for 4% chlorhexidine gluconate and 7.5% povidone iodine, while there was no cumulative effect for liquid soap. According to these results, it can be suggested that 4% chlorhexidine gluconate can be used as a reliable antiseptic agent in the hospitals, especially for laboratories, intensive care units and operating rooms, owing to its favourable immediate, residual and cumulative effects.