Phosphorylation of WAVE2 by MAP kinases regulates persistent cell migration and polarity.

PubMed

Danson, Christopher M; Pocha, Shirin M; Bloomberg, Graham B; Cory, Giles O

2007-12-01

The WAVE family of proteins has long been implicated in the stimulus-dependent generation of lamellipodia at the leading edge of migrating cells, with WAVE2 in particular implicated in the formation of peripheral ruffles and chemotactic migration. However, the lack of direct visualisation of cell migration in WAVE2 mutants or knockdowns has made defining the mechanisms of WAVE2 regulation during cell migration difficult. We have characterised three MAP kinase phosphorylation sites within WAVE2 and analysed fibroblast behaviour in a scratch-wound model following introduction of transgenes encoding phospho-defective WAVE2. The cells exhibited an increase in migration speed, a decrease in the persistence of migration, and disruption of polarisation of the Golgi apparatus. All these effects could be mimicked by acute knockdown of endogenous WAVE2 expression with RNAi, indicating that phosphorylation of WAVE2 by MAP kinases regulates cell polarity during migration.

Non-contact rapid optical coherence elastography by high-speed 4D imaging of elastic waves

NASA Astrophysics Data System (ADS)

Song, Shaozhen; Yoon, Soon Joon; Ambroziński, Łukasz; Pelivanov, Ivan; Li, David; Gao, Liang; Shen, Tueng T.; O'Donnell, Matthew; Wang, Ruikang K.

2017-02-01

Shear wave OCE (SW-OCE) uses an OCT system to track propagating mechanical waves, providing the information needed to map the elasticity of the target sample. In this study we demonstrate high speed, 4D imaging to capture transient mechanical wave propagation. Using a high-speed Fourier domain mode-locked (FDML) swept-source OCT (SS-OCT) system operating at 1.62 MHz A-line rate, the equivalent volume rate of mechanical wave imaging is 16 kvps (kilo-volumes per second), and total imaging time for a 6 x 6 x 3 mm volume is only 0.32 s. With a displacement sensitivity of 10 nanometers, the proposed 4D imaging technique provides sufficient temporal and spatial resolution for real-time optical coherence elastography (OCE). Combined with a new air-coupled, high-frequency focused ultrasound stimulator requiring no contact or coupling media, this near real-time system can provide quantitative information on localized viscoelastic properties. SW-OCE measurements are demonstrated on tissue-mimicking phantoms and porcine cornea under various intra-ocular pressures. In addition, elasticity anisotropy in the cornea is observed. Images of the mechanical wave group velocity, which correlates with tissue elasticity, show velocities ranging from 4-20 m/s depending on pressure and propagation direction. These initial results strong suggest that 4D imaging for real-time OCE may enable high-resolution quantitative mapping of tissue biomechanical properties in clinical applications.

High speed all optical shear wave imaging optical coherence elastography (Conference Presentation)

NASA Astrophysics Data System (ADS)

Song, Shaozhen; Hsieh, Bao-Yu; Wei, Wei; Shen, Tueng; O'Donnell, Matthew; Wang, Ruikang K.

2016-03-01

Optical Coherence Elastography (OCE) is a non-invasive testing modality that maps the mechanical property of soft tissues with high sensitivity and spatial resolution using phase-sensitive optical coherence tomography (PhS-OCT). Shear wave OCE (SW-OCE) is a leading technique that relies on the speed of propagating shear waves to provide a quantitative elastography. Previous shear wave imaging OCT techniques are based on repeated M-B scans, which have several drawbacks such as long acquisition time and repeated wave stimulations. Recent developments of Fourier domain mode-locked high-speed swept-source OCT system has enabled enough speed to perform KHz B-scan rate OCT imaging. Here we propose ultra-high speed, single shot shear wave imaging to capture single-shot transient shear wave propagation to perform SW-OCE. The frame rate of shear wave imaging is 16 kHz, at A-line rate of ~1.62 MHz, which allows the detection of high-frequency shear wave of up to 8 kHz. The shear wave is generated photothermal-acoustically, by ultra-violet pulsed laser, which requires no contact to OCE subjects, while launching high frequency shear waves that carries rich localized elasticity information. The image acquisition and processing can be performed at video-rate, which enables real-time 3D elastography. SW-OCE measurements are demonstrated on tissue-mimicking phantoms and porcine ocular tissue. This approach opens up the feasibility to perform real-time 3D SW-OCE in clinical applications, to obtain high-resolution localized quantitative measurement of tissue biomechanical property.

Development and Application of a Three-Dimensional Seismo-Acoustic Coupled-Mode Model

DTIC Science & Technology

2015-09-30

performance of the algorithm is evaluated through comparison with other mode finding techniques and propagation codes. It is also used to calculate...2φ 1 ∂ 2ψ ∇2φ = ∂ t2 , ∇2ψ = c2 p c2 s ∂ t2 where cp is the compressional wave speed and cs is the shear wave speed. The displacements and...19, 2015. George V. Frisk, Kyle M. Becker , Subramaniam D. Rajan, Cynthia J. Sellers, Chad M. Smith, and Megan S. Ballard, “Modal Mapping Experiment

Identification of Stratospheric Waves in Ozone in the Tropics from OMI High Spectral Resolution Measurements

NASA Technical Reports Server (NTRS)

Ziemke, J. R.; Liu, X.; Bhartia, P. K.

2007-01-01

Previous studies using Total Ozone Mapping Spectrometer (TOMS) measurements have identified several types of tropical waves in the stratosphere. These waves include Kelvin waves, mixed Rossby-gravity waves, equatorial Rossby waves, and global normal modes. All of these detected waves occur when their zonal phase speeds are opposite the zonal winds in the low-mid stratosphere associated with the Quasi-biennial Oscillation (QBO). Peak-to-peak amplitudes in all cases are typically 5 DU. While total ozone data from TOMS is sensitive in detecting these tropical waves, they provide each day only a single horizontal cross-sectional map. The high spatial and spectral resolution of the Aura Ozone Monitoring Instrument (OMI) provides a unique means to evaluate 3D structure in these waves including their propagation characteristics. Ozone profiles retrieved from OMI radiances for wavelengths 270-310 nm are utilized to examine the nature of these wave disturbances extending from the lower to upper stratosphere.

A New Comprehensive Model for Crustal and Upper Mantle Structure of the European Plate

NASA Astrophysics Data System (ADS)

Morelli, A.; Danecek, P.; Molinari, I.; Postpischl, L.; Schivardi, R.; Serretti, P.; Tondi, M. R.

2009-12-01

We present a new comprehensive model of crustal and upper mantle structure of the whole European Plate — from the North Atlantic ridge to Urals, and from North Africa to the North Pole — describing seismic speeds (P and S) and density. Our description of crustal structure merges information from previous studies: large-scale compilations, seismic prospection, receiver functions, inversion of surface wave dispersion measurements and Green functions from noise correlation. We use a simple description of crustal structure, with laterally-varying sediment and cristalline layers thickness and seismic parameters. Most original information refers to P-wave speed, from which we derive S speed and density from scaling relations. This a priori crustal model by itself improves the overall fit to observed Bouguer anomaly maps, as derived from GRACE satellite data, over CRUST2.0. The new crustal model is then used as a constraint in the inversion for mantle shear wave speed, based on fitting Love and Rayleigh surface wave dispersion. In the inversion for transversely isotropic mantle structure, we use group speed measurements made on European event-to-station paths, and use a global a priori model (S20RTS) to ensure fair rendition of earth structure at depth and in border areas with little coverage from our data. The new mantle model sensibly improves over global S models in the imaging of shallow asthenospheric (slow) anomalies beneath the Alpine mobile belt, and fast lithospheric signatures under the two main Mediterranean subduction systems (Aegean and Tyrrhenian). We map compressional wave speed inverting ISC travel times (reprocessed by Engdahl et al.) with a non linear inversion scheme making use of finite-difference travel time calculation. The inversion is based on an a priori model obtained by scaling the 3D mantle S-wave speed to P. The new model substantially confirms images of descending lithospheric slabs and back-arc shallow asthenospheric regions, shown in other more local high-resolution tomographic studies, but covers the whole range of the European Plate. We also obtain three-dimensional mantle density structure by inversion of GRACE Bouguer anomalies locally adjusting density and the scaling relation between seismic wave speeds and density. We validate the new comprehensive model through comparison of recorded seismograms with numerical simulations based on SPECFEM3D. This work is a contribution towards the definition of a reference earth model for Europe. To this extent, in order to improve model dissemination and comparison, we propose the adoption of a common exchange format for tomographic earth models based on JSON, a lightweight data-interchange format supported by most high-level programming languages. We provide tools for manipulating and visualising models, described in this standard format, in Google Earth and GEON IDV.

Dynamic spatial filtering using a digital micromirror device for high-speed optical diffraction tomography.

PubMed

Jin, Di; Zhou, Renjie; Yaqoob, Zahid; So, Peter T C

2018-01-08

Optical diffraction tomography (ODT) is an emerging microscopy technique for three-dimensional (3D) refractive index (RI) mapping of transparent specimens. Recently, the digital micromirror device (DMD) based scheme for angle-controlled plane wave illumination has been proposed to improve the imaging speed and stability of ODT. However, undesired diffraction noise always exists in the reported DMD-based illumination scheme, which leads to a limited contrast ratio of the measurement fringe and hence inaccurate RI mapping. Here we present a novel spatial filtering method, based on a second DMD, to dynamically remove the diffraction noise. The reported results illustrate significantly enhanced image quality of the obtained interferograms and the subsequently derived phase maps. And moreover, with this method, we demonstrate mapping of 3D RI distribution of polystyrene beads as well as biological cells with high accuracy. Importantly, with the proper hardware configuration, our method does not compromise the 3D imaging speed advantage promised by the DMD-based illumination scheme. Specifically, we have been able to successfully obtain interferograms at over 1 kHz speed, which is critical for potential high-throughput label-free 3D image cytometry applications.

Influence of surface gravity waves on near wake development behind a towed model horizontal axis marine current turbine

NASA Astrophysics Data System (ADS)

Luznik, Luksa; Flack, Karen; Lust, Ethan

2016-11-01

2D PIV measurements in the near wake flow field (x/D<2) are presented for a 1/25 scale, 0.8 m diameter (D) two bladed horizontal axis tidal turbine. All measurements were obtained in the USNA 380 ft tow tank with turbine towed at a constant carriage speed (Utow = 1.68 m/s), at the nominal tip speed ratio (TSR) of 7 and incoming regular waves with a period of 2.3 seconds and 0.18 m wave height. Near wake mapping is accomplished by "tiling" phase locked individual 2D PIV fields of view (nominally 30x30 cm2) with approximately 5 cm overlap. The discussion will focus on the downstream evolution of coherent tip vortices shed by the rotor blades and their vertical/horizontal displacements by the wave induced fluctuations. This observed phenomena ultimately results in significantly increased downstream wake expansion in comparison with the same conditions without waves. Office of Naval Research.

PROGRESS TOWARDS NEXT GENERATION, WAVEFORM BASED THREE-DIMENSIONAL MODELS AND METRICS TO IMPROVE NUCLEAR EXPLOSION MONITORING IN THE MIDDLE EAST

DOE Office of Scientific and Technical Information (OSTI.GOV)

Savage, B; Peter, D; Covellone, B

2009-07-02

Efforts to update current wave speed models of the Middle East require a thoroughly tested database of sources and recordings. Recordings of seismic waves traversing the region from Tibet to the Red Sea will be the principal metric in guiding improvements to the current wave speed model. Precise characterizations of the earthquakes, specifically depths and faulting mechanisms, are essential to avoid mapping source errors into the refined wave speed model. Errors associated with the source are manifested in amplitude and phase changes. Source depths and paths near nodal planes are particularly error prone as small changes may severely affect themore » resulting wavefield. Once sources are quantified, regions requiring refinement will be highlighted using adjoint tomography methods based on spectral element simulations [Komatitsch and Tromp (1999)]. An initial database of 250 regional Middle Eastern events from 1990-2007, was inverted for depth and focal mechanism using teleseismic arrivals [Kikuchi and Kanamori (1982)] and regional surface and body waves [Zhao and Helmberger (1994)]. From this initial database, we reinterpreted a large, well recorded subset of 201 events through a direct comparison between data and synthetics based upon a centroid moment tensor inversion [Liu et al. (2004)]. Evaluation was done using both a 1D reference model [Dziewonski and Anderson (1981)] at periods greater than 80 seconds and a 3D model [Kustowski et al. (2008)] at periods of 25 seconds and longer. The final source reinterpretations will be within the 3D model, as this is the initial starting point for the adjoint tomography. Transitioning from a 1D to 3D wave speed model shows dramatic improvements when comparisons are done at shorter periods, (25 s). Synthetics from the 1D model were created through mode summations while those from the 3D simulations were created using the spectral element method. To further assess errors in source depth and focal mechanism, comparisons between the three methods were made. These comparisons help to identify problematic stations and sources which may bias the final solution. Estimates of standard errors were generated for each event's source depth and focal mechanism to identify poorly constrained events. A final, well characterized set of sources and stations will be then used to iteratively improve the wave speed model of the Middle East. After a few iterations during the adjoint inversion process, the sources will be reexamined and relocated to further reduce mapping of source errors into structural features. Finally, efforts continue in developing the infrastructure required to 'quickly' generate event kernels at the n-th iteration and invert for a new, (n+1)-th, wave speed model of the Middle East. While development of the infrastructure proceeds, initial tests using a limited number of events shows the 3D model, while showing vast improvement compared to the 1D model, still requires substantial modifications. Employing our new, full source set and iterating the adjoint inversions at successively shorter periods will lead to significant changes and refined wave speed structures of the Middle East.« less

Shear wave pulse compression for dynamic elastography using phase-sensitive optical coherence tomography

NASA Astrophysics Data System (ADS)

Nguyen, Thu-Mai; Song, Shaozhen; Arnal, Bastien; Wong, Emily Y.; Huang, Zhihong; Wang, Ruikang K.; O'Donnell, Matthew

2014-01-01

Assessing the biomechanical properties of soft tissue provides clinically valuable information to supplement conventional structural imaging. In the previous studies, we introduced a dynamic elastography technique based on phase-sensitive optical coherence tomography (PhS-OCT) to characterize submillimetric structures such as skin layers or ocular tissues. Here, we propose to implement a pulse compression technique for shear wave elastography. We performed shear wave pulse compression in tissue-mimicking phantoms. Using a mechanical actuator to generate broadband frequency-modulated vibrations (1 to 5 kHz), induced displacements were detected at an equivalent frame rate of 47 kHz using a PhS-OCT. The recorded signal was digitally compressed to a broadband pulse. Stiffness maps were then reconstructed from spatially localized estimates of the local shear wave speed. We demonstrate that a simple pulse compression scheme can increase shear wave detection signal-to-noise ratio (>12 dB gain) and reduce artifacts in reconstructing stiffness maps of heterogeneous media.

Global ERS 1 and 2 and NSCAT observations: Upwind/crosswind and upwind/downwind measurements

NASA Astrophysics Data System (ADS)

Quilfen, Y.; Chapron, B.; Bentamy, A.; Gourrion, J.; El Fouhaily, T.; Vandemark, D.

1999-05-01

This paper presents an analysis of the wind speed dependence of upwind/downwind asymmetry (UDA) and upwind-crosswind anisotropy (UCA) as derived from global C band VV-polarized ERS 1 and 2 and Ku band VV- and HH-polarized NASA scatterometer (NSCAT) data. Interpretation of the results relies on identifying relationships between the differing frequencies and incidence angles that are consistent with Bragg scattering theory from gravity-capillary waves. It is shown that globally derived parameters characterizing UDA and UCA hold information on the wind dependence of short gravity and gravity-capillary wave growth and dissipation. In particular, the UCA behavior is found quadratic for both the C and Ku band, peaking at moderate wind speeds. In addition, the dual-frequency results appear to map out the expected, more rapid adjustment of centimeter-scale (Ku band) waves to the wind direction at light winds. However, as wind increases, the directionality associated with these shorter waves saturates at a lower speed than for the slightly longer waves inferred at C band. It is suggested that this observed phenomenon may be related to increasing wave-drift interactions that can potentially inhibit short-scale surface wave growth along the wind direction. Concerning UDA properties, our present analysis reveals that the NSCAT and ERS 1 and 2 scatterometers give quite different results. Our preliminary interpretation is that C band measurements may be easier to interpret using composite Bragg scattering theory and that upwind/downwind contrasts are mainly supported by short gravity waves.

From supersonic shear wave imaging to full-field optical coherence shear wave elastography

NASA Astrophysics Data System (ADS)

Nahas, Amir; Tanter, Mickaël; Nguyen, Thu-Mai; Chassot, Jean-Marie; Fink, Mathias; Claude Boccara, A.

2013-12-01

Elasticity maps of tissue have proved to be particularly useful in providing complementary contrast to ultrasonic imaging, e.g., for cancer diagnosis at the millimeter scale. Optical coherence tomography (OCT) offers an endogenous contrast based on singly backscattered optical waves. Adding complementary contrast to OCT images by recording elasticity maps could also be valuable in improving OCT-based diagnosis at the microscopic scale. Static elastography has been successfully coupled with full-field OCT (FF-OCT) in order to realize both micrometer-scale sectioning and elasticity maps. Nevertheless, static elastography presents a number of drawbacks, mainly when stiffness quantification is required. Here, we describe the combination of two methods: transient elastography, based on speed measurements of shear waves induced by ultrasonic radiation forces, and FF-OCT, an en face OCT approach using an incoherent light source. The use of an ultrafast ultrasonic scanner and an ultrafast camera working at 10,000 to 30,000 images/s made it possible to follow shear wave propagation with both modalities. As expected, FF-OCT is found to be much more sensitive than ultrafast ultrasound to tiny shear vibrations (a few nanometers and micrometers, respectively). Stiffness assessed in gel phantoms and an ex vivo rat brain by FF-OCT is found to be in good agreement with ultrasound shear wave elastography.

Generation of remote adaptive torsional shear waves with an octagonal phased array to enhance displacements and reduce variability of shear wave speeds: comparison with quasi-plane shear wavefronts.

PubMed

Ouared, Abderrahmane; Montagnon, Emmanuel; Cloutier, Guy

2015-10-21

A method based on adaptive torsional shear waves (ATSW) is proposed to overcome the strong attenuation of shear waves generated by a radiation force in dynamic elastography. During the inward propagation of ATSW, the magnitude of displacements is enhanced due to the convergence of shear waves and constructive interferences. The proposed method consists in generating ATSW fields from the combination of quasi-plane shear wavefronts by considering a linear superposition of displacement maps. Adaptive torsional shear waves were experimentally generated in homogeneous and heterogeneous tissue mimicking phantoms, and compared to quasi-plane shear wave propagations. Results demonstrated that displacement magnitudes by ATSW could be up to 3 times higher than those obtained with quasi-plane shear waves, that the variability of shear wave speeds was reduced, and that the signal-to-noise ratio of displacements was improved. It was also observed that ATSW could cause mechanical inclusions to resonate in heterogeneous phantoms, which further increased the displacement contrast between the inclusion and the surrounding medium. This method opens a way for the development of new noninvasive tissue characterization strategies based on ATSW in the framework of our previously reported shear wave induced resonance elastography (SWIRE) method proposed for breast cancer diagnosis.

USING RUNNING DIFFERENCE IMAGES TO TRACK PROPER MOTIONS OF XUV CORONAL INTENSITY ON THE SUN

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sheeley, N. R. Jr.; Warren, H. P.; Lee, J., E-mail: neil.sheeley@nrl.navy.mil, E-mail: harry.warren@nrl.navy.mil

2014-12-20

We have developed a procedure for observing and tracking proper motions of faint XUV coronal intensity on the Sun and have applied this procedure to study the collective motions of cellular plumes and the shorter-period waves in sunspots. Our space/time maps of cellular plumes show a series of tracks with the same 5-8 minute repetition times and ∼100 km s{sup –1} sky-plane speeds found previously in active-region fans and in coronal hole plumes. By synchronizing movies and space/time maps, we find that the tracks are produced by elongated ejections from the unipolar flux concentrations at the bases of the cellular plumes and thatmore » the phases of these ejections are uncorrelated from cell to cell. Thus, the large-scale motion is not a continuous flow, but is more like a system of independent conveyor belts all moving in the same direction along the magnetic field. In contrast, the proper motions in sunspots are clearly waves resulting from periodic disturbances in the sunspot umbras. The periods are ∼2.6 minutes, but the sky-plane speeds and wavelengths depend on the heights of the waves above the sunspot. In the chromosphere, the waves decelerate from 35-45 km s{sup –1} in the umbra to 7-8 km s{sup –1} toward the outer edge of the penumbra, but in the corona, the waves accelerate to ∼60-100 km s{sup –1}. Because chromospheric and coronal tracks originate from the same space/time locations, the coronal waves must emerge from the same umbral flashes that produce the chromospheric waves.« less

Multi-mode phase speed measurements with array-based analysis: Application to the North American continent

NASA Astrophysics Data System (ADS)

Matsuzawa, H.; Yoshizawa, K.

2017-12-01

Recent high-density broad-band seismic networks allow us to construct improved 3-D upper mantle models with unprecedented horizontal resolution using surface waves. Such dispersion measurements have been primarily based on the analysis of fundamental mode. Higher-mode information can be of help in enhancing vertical resolution of 3-D models, but their dispersion analysis is intrinsically difficult, since wave-packets of several modes are overlapped each other in an observed seismogram. In this study, we measure phase dispersion of multi-mode surface waves with an array-based analysis. Our method is modeled on a one-dimensional frequency-wavenumber method originally developed by Nolet (1975, GRL), which can be applied to a set of broadband seismic records observed in a linear array along a great circle path. Through this analysis, we can obtain a spectrogram in c-T (phase speed - period) domain, which is characterized by mode-branch dispersion curves and relative spectral powers for each mode. Synthetic experiments indicate that we can separate the modal contribution using a long linear array with typical array length of about 2000 to 4000 km. The method is applied to a large data set from USArray using nearly 400 seismic events in 2007 - 2014 with Mw 6.5 or greater. Our phase-speed maps for the fundamental-mode Love and Rayleigh waves and the first higher-mode Rayleigh waves match well with the earlier models. The phase speed maps reflect typical large-scale features of regional seismic structure in North America, but smaller-scale variations are less constrained in our model, since our measured phase speeds represent path-average features over a long path (about a few thousands kilometers). Our multi-mode dispersion measurements can also be used for the extraction of mode-branch waveforms for the first a few modes. This can be done by applying a narrow filter around the dispersion curves of a target mode in c-T spectrogram. The mode-branch waveforms can then be reconstructed based on a linear Radon transform (e.g., Luo et al., 2015, GJI). Synthetic experiments suggest that we can successfully retrieve the mode-branch waveforms for several mode branches, which can be used in the secondary analysis for constraining local-scale heterogeneity with enhanced depth resolution.

Tracking the Progress of EarthScope/USArray: The crust and upper mantle beneath the transition region between tectonic western US and cratonic eastern US

NASA Astrophysics Data System (ADS)

Shen, W.; Lin, F.; Ritzwoller, M. H.

2010-12-01

The transition region between the tectonic western US and the cratonic eastern US contains numerous significant geological regions (e.g., the Rocky Mountains, the Colorado Plateau, and the Rio Grande Rift), and also, unknowns (e.g, the location or extent of the east-west US dichotomy, the compensation of the high topography of the western Great Plains, the extensional mechanics of the Rio Grande Rift, and the structure of the mantle beneath the Colorado Plateau). The answers to these questions and others are critical to an understanding of the tectonics and tectonic history of this region and its impact on the cratonic eastern US. The recent deployments of seismic stations, particularly the EarthScope USArray Transportable Array (TA), provide an opportunity to construct a detailed 3-D structural model of the crust and upper mantle beneath this transition region, and thus allow us to address some of the questions listed above. We present results from ambient noise tomography (ANT) and teleseismic earthquake tomography by using data from TA stations within the western and central US. We processed continuous seismic noise data from ~600 TA stations from August 2008 to March 2010, which after data selection produces a data set with ~100,000 inter-station paths. Rayleigh wave phase speed maps between 6 and 40 sec period and Love wave phase speed maps between 8 and 30 sec with a resolution of ~60 km are constructed using eikonal tomography. In addition, we applied eikonal tomography (ET) to about 300 teleseismic earthquakes to obtain long-period (30 - 100 sec) Rayleigh wave phase speed maps and Love wave phase speeds maps (30 - 60 sec). By jointly inverting Rayleigh and Love phase speeds maps from ANT and earthquake tomography, we constructed a 3-D isotropic and radially anisotropic shear velocity model of the crust and upper mantle to ~150 km depth together with model uncertainties constrained by a Monte-Carlo inversion. The 3-D isotropic model reveals a variety of robust features in this transition region. In the uppermost crust, the main sedimentary basins (e.g., Green River, Uinta, Washakie, Powder River, Denver, Albuquerque, Permian, and Anadarko) are imaged. In the middle and lower crust where the low shear velocities from basins diminish, the Yellowstone hot spot becomes the main slow anomaly. In the uppermost mantle, high velocity anomalies are observed beneath the Colorado Plateau, the Wyoming craton, and the Great Plains. Although the Colorado Plateau shows more or less homogeneous shear velocity in its middle and towards its northern boundary, the other two main fast anomalies reveal inhomogeneous structures at depths deeper than 100 km. Two main low velocity anomalies are observed: one underlying the Snake River Plain which broadens and dips to the northeast and another U-shaped anomaly on the eastern margin of the Colorado Plateau. These velocity anomalies add to complexities at the transition between the tectonic western US and the stable eastern US. The location and uncertainty of the east-west shear velocity dichotomy also is constrained by this model.

Wave rotor demonstrator engine assessment

NASA Technical Reports Server (NTRS)

Snyder, Philip H.

1996-01-01

The objective of the program was to determine a wave rotor demonstrator engine concept using the Allison 250 series engine. The results of the NASA LERC wave rotor effort were used as a basis for the wave rotor design. A wave rotor topped gas turbine engine was identified which incorporates five basic requirements of a successful demonstrator engine. Predicted performance maps of the wave rotor cycle were used along with maps of existing gas turbine hardware in a design point study. The effects of wave rotor topping on the engine cycle and the subsequent need to rematch compressor and turbine sections in the topped engine were addressed. Comparison of performance of the resulting engine is made on the basis of wave rotor topped engine versus an appropriate baseline engine using common shaft compressor hardware. The topped engine design clearly demonstrates an impressive improvement in shaft horsepower (+11.4%) and SFC (-22%). Off design part power engine performance for the wave rotor topped engine was similarly improved including that at engine idle conditions. Operation of the engine at off design was closely examined with wave rotor operation at less than design burner outlet temperatures and rotor speeds. Challenges identified in the development of a demonstrator engine are discussed. A preliminary design was made of the demonstrator engine including wave rotor to engine transition ducts. Program cost and schedule for a wave rotor demonstrator engine fabrication and test program were developed.

GEOS-3 radar altimeter study for the South Atlantic Bight

NASA Technical Reports Server (NTRS)

Leitao, C. D.; Huang, N.; Parsons, C. L.; Parra, C. G.; Mcmill, J. D.; Hayes, G. S.

1980-01-01

Three years of radar altimeter data from GEOS-3 for the South Atlantic Bight were processed. Mean monthly topographic maps were produced which estimate geostrophic flow in the region. Statistical distribution of the surface wind speed and significant wave height as a function of both space and time are presented.

Time-Distance Helioseismology Data-Analysis Pipeline for Helioseismic and Magnetic Imager Onboard Solar Dynamics Observatory (SDO-HMI) and Its Initial Results

NASA Technical Reports Server (NTRS)

Zhao, J.; Couvidat, S.; Bogart, R. S.; Parchevsky, K. V.; Birch, A. C.; Duvall, Thomas L., Jr.; Beck, J. G.; Kosovichev, A. G.; Scherrer, P. H.

2011-01-01

The Helioseismic and Magnetic Imager onboard the Solar Dynamics Observatory (SDO/HMI) provides continuous full-disk observations of solar oscillations. We develop a data-analysis pipeline based on the time-distance helioseismology method to measure acoustic travel times using HMI Doppler-shift observations, and infer solar interior properties by inverting these measurements. The pipeline is used for routine production of near-real-time full-disk maps of subsurface wave-speed perturbations and horizontal flow velocities for depths ranging from 0 to 20 Mm, every eight hours. In addition, Carrington synoptic maps for the subsurface properties are made from these full-disk maps. The pipeline can also be used for selected target areas and time periods. We explain details of the pipeline organization and procedures, including processing of the HMI Doppler observations, measurements of the travel times, inversions, and constructions of the full-disk and synoptic maps. Some initial results from the pipeline, including full-disk flow maps, sunspot subsurface flow fields, and the interior rotation and meridional flow speeds, are presented.

Optical coherence tomography detection of shear wave propagation in inhomogeneous tissue equivalent phantoms and ex-vivo carotid artery samples

PubMed Central

Razani, Marjan; Luk, Timothy W.H.; Mariampillai, Adrian; Siegler, Peter; Kiehl, Tim-Rasmus; Kolios, Michael C.; Yang, Victor X.D.

2014-01-01

In this work, we explored the potential of measuring shear wave propagation using optical coherence elastography (OCE) in an inhomogeneous phantom and carotid artery samples based on a swept-source optical coherence tomography (OCT) system. Shear waves were generated using a piezoelectric transducer transmitting sine-wave bursts of 400 μs duration, applying acoustic radiation force (ARF) to inhomogeneous phantoms and carotid artery samples, synchronized with a swept-source OCT (SS-OCT) imaging system. The phantoms were composed of gelatin and titanium dioxide whereas the carotid artery samples were embedded in gel. Differential OCT phase maps, measured with and without the ARF, detected the microscopic displacement generated by shear wave propagation in these phantoms and samples of different stiffness. We present the technique for calculating tissue mechanical properties by propagating shear waves in inhomogeneous tissue equivalent phantoms and carotid artery samples using the ARF of an ultrasound transducer, and measuring the shear wave speed and its associated properties in the different layers with OCT phase maps. This method lays the foundation for future in-vitro and in-vivo studies of mechanical property measurements of biological tissues such as vascular tissues, where normal and pathological structures may exhibit significant contrast in the shear modulus. PMID:24688822

Ultrasound elastic tensor imaging: comparison with MR diffusion tensor imaging in the myocardium

NASA Astrophysics Data System (ADS)

Lee, Wei-Ning; Larrat, Benoît; Pernot, Mathieu; Tanter, Mickaël

2012-08-01

We have previously proven the feasibility of ultrasound-based shear wave imaging (SWI) to non-invasively characterize myocardial fiber orientation in both in vitro porcine and in vivo ovine hearts. The SWI-estimated results were in good correlation with histology. In this study, we proposed a new and robust fiber angle estimation method through a tensor-based approach for SWI, coined together as elastic tensor imaging (ETI), and compared it with magnetic resonance diffusion tensor imaging (DTI), a current gold standard and extensively reported non-invasive imaging technique for mapping fiber architecture. Fresh porcine (n = 5) and ovine (n = 5) myocardial samples (20 × 20 × 30 mm3) were studied. ETI was firstly performed to generate shear waves and to acquire the wave events at ultrafast frame rate (8000 fps). A 2.8 MHz phased array probe (pitch = 0.28 mm), connected to a prototype ultrasound scanner, was mounted on a customized MRI-compatible rotation device, which allowed both the rotation of the probe from -90° to 90° at 5° increments and co-registration between two imaging modalities. Transmural shear wave speed at all propagation directions realized was firstly estimated. The fiber angles were determined from the shear wave speed map using the least-squares method and eigen decomposition. The test myocardial sample together with the rotation device was then placed inside a 7T MRI scanner. Diffusion was encoded in six directions. A total of 270 diffusion-weighted images (b = 1000 s mm-2, FOV = 30 mm, matrix size = 60 × 64, TR = 6 s, TE = 19 ms, 24 averages) and 45 B0 images were acquired in 14 h 30 min. The fiber structure was analyzed by the fiber-tracking module in software, MedINRIA. The fiber orientation in the overlapped myocardial region which both ETI and DTI accessed was therefore compared, thanks to the co-registered imaging system. Results from all ten samples showed good correlation (r2 = 0.81, p < 0.0001) and good agreement (3.05° bias) between ETI and DTI fiber angle estimates. The average ETI-estimated fractional anisotropy (FA) values decreased from subendocardium to subepicardium (p < 0.05, unpaired, one-tailed t-test, N = 10) by 33%, whereas the corresponding DTI-estimated FA values presented a change of -10% (p > 0.05, unpaired, one-tailed t-test, N = 10). In conclusion, we have demonstrated that the fiber orientation estimated by ETI, which assesses the shear wave speed (and thus the stiffness), was comparable to that measured by DTI, which evaluates the preferred direction of water diffusion, and have validated this concept within the myocardium. Moreover, ETI was shown capable of mapping the transmural fiber angles with as few as seven shear wave propagation directions.

Observations of a bi-directional lightning leader producing an M-component

NASA Astrophysics Data System (ADS)

Kotovsky, D. A.; Uman, M. A.; Wilkes, R.; Carvalho, F. L.; Jordan, D. M.

2017-12-01

Lightning discharges to ground often exhibit millisecond-scale surges in the continuing currents following return strokes, called M-components. Relatively little is known regarding the source of M-component charge and the mechanisms by which that charge is transferred to ground. In this work, we seek to directly address these questions by presenting correlated high-speed video and Lightning Mapping Array (LMA) observations of a bi-directional leader that resulted in an M-component occurring in a rocket-and-wire triggered lightning flash. The observed leader initiated in the decayed remnants of a positive leader channel that had traversed virgin air approximately 90 msec prior. Three-dimensional locations and speeds of the photographed bi-directional leader and M-component processes are calculated by mapping video images to the observed LMA channel geometry. Both ends of the bi-directional leader exhibited speeds on the order of 2 x106 m sec-1 over 570 meters of the visible channel. Propagation of the luminosity wave from the in-cloud leader to ground ( 8.8 km channel length) exhibited appreciable dispersion, with rise-times (10-90%) increasing from 330 to 410 μsec and pulse-widths (half-maximum) increasing from 380 to 810 μsec - the M-component current pulse measured at ground-level exhibited a rise-time of 290 μsec and a pulse-width of 770 μsec. Group velocities of the luminosity wave have been calculated as a function of frequency, increasing from 2 x107 to 6 x107 m sec-1 over the dominant signal bandwidth (DC to 2 kHz). Additionally, multiple waves of luminosity are observed within the in-cloud channel, indicating nuanced wave phenomena possibly associated with reflection from the end of the leader channel and attachment with the main lightning channel carrying continuing current to ground.

Variability of electromagnetic signatures of lightning initiation observed in the West Mediterranean basin

NASA Astrophysics Data System (ADS)

Kolmasova, I.; Santolik, O.; Defer, E.; Stéphane, P.; Lan, R.; Uhlir, L.; Coquillat, S.; Lambert, D.; Pinty, J. P.; Prieur, S.

2016-12-01

Lightning discharges to ground often exhibit millisecond-scale surges in the continuing currents following return strokes, called M-components. Relatively little is known regarding the source of M-component charge and the mechanisms by which that charge is transferred to ground. In this work, we seek to directly address these questions by presenting correlated high-speed video and Lightning Mapping Array (LMA) observations of a bi-directional leader that resulted in an M-component occurring in a rocket-and-wire triggered lightning flash. The observed leader initiated in the decayed remnants of a positive leader channel that had traversed virgin air approximately 90 msec prior. Three-dimensional locations and speeds of the photographed bi-directional leader and M-component processes are calculated by mapping video images to the observed LMA channel geometry. Both ends of the bi-directional leader exhibited speeds on the order of 2 x106 m sec-1 over 570 meters of the visible channel. Propagation of the luminosity wave from the in-cloud leader to ground ( 8.8 km channel length) exhibited appreciable dispersion, with rise-times (10-90%) increasing from 330 to 410 μsec and pulse-widths (half-maximum) increasing from 380 to 810 μsec - the M-component current pulse measured at ground-level exhibited a rise-time of 290 μsec and a pulse-width of 770 μsec. Group velocities of the luminosity wave have been calculated as a function of frequency, increasing from 2 x107 to 6 x107 m sec-1 over the dominant signal bandwidth (DC to 2 kHz). Additionally, multiple waves of luminosity are observed within the in-cloud channel, indicating nuanced wave phenomena possibly associated with reflection from the end of the leader channel and attachment with the main lightning channel carrying continuing current to ground.

Passive optical coherence elastography using a time-reversal approach (Conference Presentation)

NASA Astrophysics Data System (ADS)

Nguyen, Thu-Mai; Zorgani, Ali; Fink, Mathias; Catheline, Stefan; Boccara, A. Claude

2017-02-01

Background and motivation - Conventional Optical Coherence Elastography (OCE) methods consist in launching controlled shear waves in tissues, and measuring their propagation speed using an ultrafast imaging system. However, the use of external shear sources limits transfer to clinical practice, especially for ophthalmic applications. Here, we propose a totally passive OCE method for ocular tissues based on time-reversal of the natural vibrations. Methods - Experiments were first conducted on a tissue-mimicking phantom containing a stiff inclusion. Pulsatile motions were reproduced by stimulating the phantom surface with two piezoelectric actuators excited asynchronously at low frequencies (50-500 Hz). The resulting random displacements were tracked at 190 frames/sec using spectral-domain optical coherence tomography (SD-OCT), with a 10x5µm² resolution over a 3x2mm² field-of-view (lateral x depth). The shear wavefield was numerically refocused (i.e. time-reversed) at each pixel using noise-correlation algorithms. The focal spot size yields the shear wavelength. Results were validated by comparison with shear wave speed measurements obtained from conventional active OCE. In vivo tests were then conducted on anesthetized rats. Results - The stiff inclusion of the phantom was delineated on the wavelength map with a wavelength ratio between the inclusion and the background (1.6) consistent with the speed ratio (1.7). This validates the wavelength measurements. In vivo, natural shear waves were detected in the eye and wavelength maps of the anterior segment showed a clear elastic contrast between the cornea, the sclera and the iris. Conclusion - We validated the time-reversal approach for passive elastography using SD-OCT imaging at low frame-rate. This method could accelerate the clinical transfer of ocular elastography.

Application of Ifsar Technology in Topographic Mapping: JUPEM's Experience

NASA Astrophysics Data System (ADS)

Zakaria, Ahamad

2018-05-01

The application of Interferometric Synthetic Aperture Radar (IFSAR) in topographic mapping has increased during the past decades. This is due to the advantages that IFSAR technology offers in solving data acquisition problems in tropical regions. Unlike aerial photography, radar technology offers wave penetration through cloud cover, fog and haze. As a consequence, images can be made free of any natural phenomenon defects. In Malaysia, Department of Survey and Mapping Malaysia (JUPEM) has been utilizing the IFSAR products since 2009 to update topographic maps at 1 : 50,000 map scales. Orthorectified radar imagery (ORI), Digital Surface Models (DSM) and Digital Terrain Models (DTM) procured under the project have been further processed before the products are ingested into a revamped mapping workflow consisting of stereo and mono digitizing processes. The paper will highlight the experience of Department of Survey and Mapping Malaysia (DSMM)/ JUPEM in using such technology in order to speed up mapping production.

Relationship between the upper mantle high velocity seismic lid and the continental lithosphere

NASA Astrophysics Data System (ADS)

Priestley, Keith; Tilmann, Frederik

2009-04-01

The lithosphere-asthenosphere boundary corresponds to the base of the "rigid" plates - the depth at which heat transport changes from advection in the convecting deeper upper mantle to conduction in the shallow upper mantle. Although this boundary is a fundamental feature of the Earth, mapping it has been difficult because it does not correspond to a sharp change in temperature or composition. Various definitions of the lithosphere and asthenosphere are based on the analysis of different types of geophysical and geological observations. The depth to the lithosphere-asthenosphere boundary determined from these different observations often shows little agreement when they are applied to the same region because the geophysical and geological observations (i.e., seismic velocity, strain rate, electrical resistivity, chemical depletion, etc.) are proxies for the change in rheological properties rather than a direct measure of the rheological properties. In this paper, we focus on the seismic mapping of the upper mantle high velocity lid and low velocity zone and its relationship to the lithosphere and asthenosphere. We have two goals: (a) to examine the differences in how teleseismic body-wave travel-time tomography and surface-wave tomography image upper mantle seismic structure; and (b) to summarise how upper mantle seismic velocity structure can be related to the structure of the lithosphere and asthenosphere. Surface-wave tomography provides reasonably good depth resolution, especially when higher modes are included in the analysis, but lateral resolution is limited by the horizontal wavelength of the long-period surface waves used to constrain upper mantle velocity structure. Teleseismic body-wave tomography has poor depth resolution in the upper mantle, particularly when no strong lateral contrasts are present. If station terms are used, features with large lateral extent and gradual boundaries are attenuated in the tomographic image. Body-wave models are not useful in mapping the thickness of the high velocity upper mantle lid because this type of analysis often determines wave speed perturbations from an unknown horizontal average and not absolute velocities. Thus, any feature which extends laterally across the whole region beneath a seismic network becomes invisible in the teleseismic body-wave tomographic image. We compare surface-wave and body-wave tomographic results using southern Africa as an example. Surface-wave tomographic images for southern Africa show a strong, high velocity upper mantle lid confined to depths shallower than ~ 200 km, whereas body-wave tomographic images show weak high velocity in the upper mantle extending to depths of ~ 300 km or more. However, synthetic tests show that these results are not contradictory. The absolute seismic velocity structure of the upper mantle provided by surface wave analysis can be used to map the thermal lithosphere. Priestley and McKenzie (Priestley, K., McKenzie, D., 2006. The thermal structure of the lithosphere from shear wave velocities. Earth and Planetary Science Letters 244, 285-301.) derive an empirical relationship between shear wave velocity and temperature. This relationship is used to obtain temperature profiles from the surface-wave tomographic models of the continental mantle. The base of the lithosphere is shown by a change in the gradient of the temperature profiles indicative of the depth where the mode of heat transport changes from conduction to advection. Comparisons of the geotherms determined from the conversion of surface-wave wave speeds to temperatures with upper mantle nodule-derived geotherms demonstrate that estimates of lithospheric thickness from Vs and from the nodule mineralogy agree to within about 25 km. The lithospheric thickness map for Africa derived from the surface-wave tomographic results shows that thick lithosphere underlies most of the Archean crust in Africa. The distribution of diamondiferous kimberlites provides an independent estimate of where thick lithosphere exists. Diamondiferous kimberlites generally occur where the lower part of the thermal lithosphere as indicated by seismology is in the diamond stability field.

Amplitude-modulated ultrasound radiation force combined with phase-sensitive optical coherence tomography for shear wave elastography

NASA Astrophysics Data System (ADS)

Nguyen, Thu-Mai; Song, Shaozhen; Arnal, Bastien; Wong, Emily Y.; Shen, Tueng T.; Wang, Ruikang K.; O'Donnell, Matthew

2015-03-01

Tissue stiffness can be measured from the propagation speed of shear waves. Acoustic radiation force (ARF) can generate shear waves by focusing ultrasound in tissue for ~100 μs. Safety considerations and electronics abilities limit ultrasound pressures. We previously presented shear wave elastography combining ARF and phase-sensitive optical coherence tomography (PhS-OCT) [1]. Here, we use amplitude-modulated ARF to enhance shear wave signal-to-noise ratio (SNR) at low pressures. Experiments were performed on tissue-mimicking phantoms. ARF was applied using a single-element transducer, driven by a 7.5 MHz, 3-ms, sine wave modulated in amplitude by a linear-swept frequency (1 to 7 kHz). Pressures between 1 to 3 MPa were tested. Displacements were tracked using PhS-OCT and numerically compressed using pulse compression methods detailed in previous work [2]. SNR was compared to that of 200-μs bursts. Stiffness maps were reconstructed using time-of-flight computations. 200-μs bursts give barely detectable displacements at 1 MPa (3.7 dB SNR). Pulse compression gives 36.2 dB at 1.5 MPa. In all cases with detectable displacements, shear wave speeds were determined in 5%-gelatin and 10%-gelatin phantoms and compared to literature values. Applicability to ocular tissues (cornea, intraocular lens) is under investigation.

Rayleigh wave tomography of the British Isles from ambient seismic noise

NASA Astrophysics Data System (ADS)

Nicolson, Heather; Curtis, Andrew; Baptie, Brian

2014-08-01

We present the first Rayleigh wave group speed maps of the British Isles constructed from ambient seismic noise. The maps also constitute the first surface wave tomography study of the crust under the British Isles at a relatively high resolution. We computed interferometric, interstation Rayleigh waves from vertical component records of ambient seismic noise recorded on 63 broad-band and short-period stations across the UK and Ireland. Group velocity measurements were made from the resulting surface wave dispersion curves between 5 and 25 s using a multiple phase-matched filter method. Uncertainties in the group velocities were computed by calculating the standard deviation of four dispersion curves constructed by stacking a random selection of daily cross-correlations. Where an uncertainty could not be obtained for a ray path using this method, we estimated it as a function of the interreceiver distance. Group velocity maps were computed for 5-25-s period using the Fast Marching forward solution of the eikonal equation and iterative, linearized inversion. At short and intermediate periods, the maps show remarkable agreement with the major geological features of the British Isles including: terrane boundaries in Scotland; regions of late Palaeozoic basement uplift; areas of exposed late Proterozoic/early Palaeozoic rocks in southwest Scotland, northern England and northwest Wales and, sedimentary basins formed during the Mesozoic such as the Irish Sea Basin, the Chester Basin, the Worcester Graben and the Wessex Basin. The maps also show a consistent low-velocity anomaly in the region of the Midlands Platform, a Proterozoic crustal block in the English Midlands. At longer periods, which are sensitive velocities in the lower crustal/upper mantle, the maps suggest that the depth of Moho beneath the British Isles decreases towards the north and west. Areas of fast velocity in the lower crust also coincide with areas thought to be associated with underplating of the lower crust such as Northern Ireland, the eastern Irish Sea and northwest Wales.

Uncertainty loops in travel-time tomography from nonlinear wave physics.

PubMed

Galetti, Erica; Curtis, Andrew; Meles, Giovanni Angelo; Baptie, Brian

2015-04-10

Estimating image uncertainty is fundamental to guiding the interpretation of geoscientific tomographic maps. We reveal novel uncertainty topologies (loops) which indicate that while the speeds of both low- and high-velocity anomalies may be well constrained, their locations tend to remain uncertain. The effect is widespread: loops dominate around a third of United Kingdom Love wave tomographic uncertainties, changing the nature of interpretation of the observed anomalies. Loops exist due to 2nd and higher order aspects of wave physics; hence, although such structures must exist in many tomographic studies in the physical sciences and medicine, they are unobservable using standard linearized methods. Higher order methods might fruitfully be adopted.

Role of Compressibility on Tsunami Propagation

NASA Astrophysics Data System (ADS)

Abdolali, Ali; Kirby, James T.

2017-12-01

In the present paper, we aim to reduce the discrepancies between tsunami arrival times evaluated from tsunami models and real measurements considering the role of ocean compressibility. We perform qualitative studies to reveal the phase speed reduction rate via a modified version of the Mild Slope Equation for Weakly Compressible fluid (MSEWC) proposed by Sammarco et al. (2013). The model is validated against a 3-D computational model. Physical properties of surface gravity waves are studied and compared with those for waves evaluated from an incompressible flow solver over realistic geometry for 2011 Tohoku-oki event, revealing reduction in phase speed.