Sample records for x-23 sv-5 program

  1. Fetal bovine serum and human constitutive androstane receptor: Evidence for activation of the SV23 splice variant by artemisinin, artemether, and arteether in a serum-free cell culture system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lau, Aik Jiang; Chang, Thomas K.H., E-mail: thomas.chang@ubc.ca

    The naturally occurring SV23 splice variant of human constitutive androstane receptor (hCAR-SV23) is activated by di-(2-ethylhexyl)phthalate (DEHP), which is detected as a contaminant in fetal bovine serum (FBS). In our initial experiment, we compared the effect of dialyzed FBS, charcoal-stripped, dextran-treated FBS (CS-FBS), and regular FBS on the basal activity and ligand-activation of hCAR-SV23 in a cell-based reporter gene assay. In transfected HepG2 cells cultured in medium supplemented with 10% FBS, basal hCAR-SV23 activity varied with the type of FBS (regular > dialyzed > CS). DEHP increased hCAR-SV23 activity when 10% CS-FBS, but not regular FBS or dialyzed FBS, wasmore » used. With increasing concentrations (1–10%) of regular FBS or CS-FBS, hCAR-SV23 basal activity increased, whereas in DEHP-treated cells, hCAR-SV23 activity remained similar (regular FBS) or slightly increased (CS-FBS). Subsequent experiments identified a serum-free culture condition to detect DEHP activation of hCAR-SV23. Under this condition, artemisinin, artemether, and arteether increased hCAR-SV23 activity, whereas they decreased it in cells cultured in medium supplemented with 10% regular FBS. By comparison, FBS increased the basal activity of the wild-type isoform of hCAR (hCAR-WT), whereas it did not affect the basal activity of the SV24 splice variant (hCAR-SV24) or ligand activation of hCAR-SV24 and hCAR-WT by 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO). The use of serum-free culture condition was suitable for detecting CITCO activation of hCAR-WT and hCAR-SV24. In conclusion, FBS leads to erroneous classification of pharmacological ligands of hCAR-SV23 in cell-based assays, but investigations on functional ligands of hCAR isoforms can be conducted in serum-free culture condition. - Highlights: • FBS leads to erroneous pharmacological classification of hCAR-SV23 ligands. • Artemisinin, artemether, and arteether

  2. Drosophila vitelline membrane assembly: A critical role for an evolutionarily conserved cysteine in the “VM domain” of sV23

    PubMed Central

    Wu, T; Manogaran, A.L; Beauchamp, J.M.; Waring, G.L.

    2010-01-01

    The vitelline membrane (VM), the oocyte proximal layer of the Drosophila eggshell, contains four major proteins (VMPs) that possess a highly conserved “VM domain” which includes three precisely spaced, evolutionarily conserved, cysteines (CX7CX8C). Focusing on sV23, this study showed that the three cysteines are not functionally equivalent. While substitution mutations at the first (C123S) or third (C140S) cysteines were tolerated, females with a substitution at the second position (C131S) were sterile. Fractionation studies showed sV23 incorporates into a large disulfide linked network well after its secretion ceases, suggesting post-depositional mechanisms are in place to restrict disulfide bond formation until late oogenesis, when the oocyte no longer experiences large volume increases. Affinity chromatography utilizing histidine tagged sV23 alleles revealed small sV23 disulfide linked complexes during the early stages of eggshell formation that included other VMPs, namely sV17 and Vml. The early presence but late loss of these associations in an sV23 double cysteine mutant suggests reorganization of disulfide bonds may underlie the regulated growth of disulfide-linked networks in the vitelline membrane. Found within the context of a putative thioredoxin active site (CXXS) C131, the critical cysteine in sV23, may play an important enzymatic role in isomerizing intermolecular disulfide bonds during eggshell assembly. PMID:20832396

  3. 23. Photocopy of photograph (4 x 5 inch enlargement of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Photocopy of photograph (4 x 5 inch enlargement of 1940 3-1/4 x 4-1/4 inch print by R. Nevan McCullough; in Cultural Resource files, Supervisor's Office, Mt. Baker-Snoqualmie National Forest) SOUTH FRONT - Suntop Lookout, Forest Road 510, Mt. Baker-Snoqualmie National Forest, Greenwater, Pierce County, WA

  4. Strain differences in arsenic-induced oxidative lesion via arsenic biomethylation between C57BL/6J and 129X1/SvJ mice

    NASA Astrophysics Data System (ADS)

    Wu, Ruirui; Wu, Xiafang; Wang, Huihui; Fang, Xin; Li, Yongfang; Gao, Lanyue; Sun, Guifan; Pi, Jingbo; Xu, Yuanyuan

    2017-03-01

    Arsenic is a common environmental and occupational toxicant with dramatic species differences in its susceptibility and metabolism. Mouse strain variability may provide a better understanding of the arsenic pathological profile but is largely unknown. Here we investigated oxidative lesion induced by acute arsenic exposure in the two frequently used mouse strains C57BL/6J and 129X1/SvJ in classical gene targeting technique. A dose of 5 mg/kg body weight arsenic led to a significant alteration of blood glutathione towards oxidized redox potential and increased hepatic malondialdehyde content in C57BL/6J mice, but not in 129X1/SvJ mice. Hepatic antioxidant enzymes were induced by arsenic in transcription in both strains and many were higher in C57BL/6J than 129X1/SvJ mice. Arsenic profiles in the liver, blood and urine and transcription of genes encoding enzymes involved in arsenic biomethylation all indicate a higher arsenic methylation capacity, which contributes to a faster hepatic arsenic excretion, in 129X1/SvJ mice than C57BL/6J mice. Taken together, C57BL/6J mice are more susceptible to oxidative hepatic injury compared with 129X1/SvJ mice after acute arsenic exposure, which is closely associated with arsenic methylation pattern of the two strains.

  5. Specific DNA binding activity of T antigen subclasses varies among different SV40-transformed cell lines.

    PubMed

    Burger, C; Fanning, E

    1983-04-15

    Large tumor antigen (T antigen) occurs in at least three different oligomeric subclasses in cells infected or transformed by simian virus 40 (SV40): 5-7 S, 14-16 S, and 23-25 S. The 23-25 S form is complexed with a host phosphoprotein (p53). The DNA binding properties of these three subclasses of T antigen from nine different cell lines and free p53 protein were compared using an immunoprecipitation assay. All three subclasses of T antigen bound specifically to SV40 DNA sequences near the origin of replication. However, the DNA binding activity varied between different cell lines over a 40- to 50-fold range. The 23-25 S and 14-16 S forms from most of the cell lines tested bound much less SV40 origin DNA than 5-7 S T antigen. The free p53 phosphoprotein did not bind specifically to any SV40 DNA sequences.

  6. 23. Photographic copy enlargement from a 4x5 copy negative of ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. Photographic copy enlargement from a 4x5 copy negative of a drawing (Original drawing located on abandoned NASA site, currently owned by the City of Downey, Downey, Calfornia). JANUARY 1960 USAF PLANT 16 MASTER PLOT AND GRID PLAN. - NASA Industrial Plant, Missile Research Laboratory, 12214 Lakewood Boulevard, Downey, Los Angeles County, CA

  7. Transgene-free human induced pluripotent stem cell line (HS5-SV.hiPS) generated from cesarean scar-derived fibroblasts.

    PubMed

    Rungsiwiwut, Ruttachuk; Pavarajarn, Wipawee; Numchaisrika, Pranee; Virutamasen, Pramuan; Pruksananonda, Kamthorn

    2016-01-01

    Transgene-free human HS5-SV.hiPS line was generated from human cesarean scar-derived fibroblasts using temperature-sensitive Sendai virus vectors carrying Oct4, Sox2, cMyc and Klf4 exogenous transcriptional factors. The viral constructs were eliminated from HS5-SV.hiPS line through heat treatment. Transgene-free HS5-SV.hiPS cells expressed pluripotent associated transcription factors Oct4, Nanog, Sox2, Rex1 and surface markers SSEA-4, TRA-1-60 and OCT4. HS5-SV.hiPS cells formed embryoid bodies and differentiated into three embryonic germ layers in vivo. HS5-SV.hiPS cells maintained their normal karyotype (46, XX) after culture for extended period. HS5-SV.hiPS displayed the similar pattern of DNA fingerprinting to the parenteral scar-derived fibroblasts. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  8. 23 CFR 924.5 - Policy.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Policy. 924.5 Section 924.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HIGHWAY SAFETY HIGHWAY SAFETY IMPROVEMENT PROGRAM § 924.5 Policy. (a... resulting from crashes on all public roads. (b) Under 23 U.S.C. 148(a)(3), a variety of highway safety...

  9. 23 CFR 924.5 - Policy.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Policy. 924.5 Section 924.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HIGHWAY SAFETY HIGHWAY SAFETY IMPROVEMENT PROGRAM § 924.5 Policy. (a... resulting from crashes on all public roads. (b) Under 23 U.S.C. 148(a)(3), a variety of highway safety...

  10. 23 CFR 924.5 - Policy.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Policy. 924.5 Section 924.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HIGHWAY SAFETY HIGHWAY SAFETY IMPROVEMENT PROGRAM § 924.5 Policy. (a... resulting from crashes on all public roads. (b) Under 23 U.S.C. 148(a)(3), a variety of highway safety...

  11. Comparative Transcriptome Profiling of an SV40-Transformed Human Fibroblast (MRC5CVI) and Its Untransformed Counterpart (MRC-5) in Response to UVB Irradiation

    PubMed Central

    Chang, Cheng-Wei; Chen, Chaang-Ray; Huang, Chao-Ying; Shu, Wun-Yi; Chiang, Chi-Shiun; Hong, Ji-Hong; Hsu, Ian C.

    2013-01-01

    Simian virus 40 (SV40) transforms cells through the suppression of tumor-suppressive responses by large T and small t antigens; studies on the effects of these two oncoproteins have greatly improved our knowledge of tumorigenesis. Large T antigen promotes cellular transformation by binding and inactivating p53 and pRb tumor suppressor proteins. Previous studies have shown that not all of the tumor-suppressive responses were inactivated in SV40-transformed cells; however, the underlying cause is not fully studied. In this study, we investigated the UVB-responsive transcriptome of an SV40-transformed fibroblast (MRC5CVI) and that of its untransformed counterpart (MRC-5). We found that, in response to UVB irradiation, MRC-5 and MRC5CVI commonly up-regulated the expression of oxidative phosphorylation genes. MRC-5 up-regulated the expressions of chromosome condensation, DNA repair, cell cycle arrest, and apoptotic genes, but MRC5CVI did not. Further cell death assays indicated that MRC5CVI was more sensitive than MRC-5 to UVB-induced cell death with increased caspase-3 activation; combining with the transcriptomic results suggested that MRC5CVI may undergo UVB-induced cell death through mechanisms other than transcriptional regulation. Our study provides a further understanding of the effects of SV40 transformation on cellular stress responses, and emphasizes the value of SV40-transformed cells in the researches of sensitizing neoplastic cells to radiations. PMID:24019915

  12. Genetic influences on ovulation of primary oocytes in LT/Sv strain mice.

    PubMed

    Everett, Clare A; Auchincloss, Catherine A; Kaufman, Matthew H; Abbott, Catherine M; West, John D

    2004-11-01

    A high proportion of LT/Sv strain oocytes arrest in meiotic metaphase I (MI) and are ovulated as diploid primary oocytes rather than haploid secondary oocytes. (Mus musculus castaneus x LT/SvKau)F1 x LT/SvKau backcross females were analysed for the proportion of oocytes that arrested in MI and typed by PCR for a panel of microsatellite DNA sequences (simple sequence repeat polymorphisms) that differed between strain LT/SvKau and M. m. castaneus. This provided a whole genome scan of 86 genetic markers distributed over all 19 autosomes and the X chromosome, and revealed genetic linkage of the MI arrest phenotype to markers on chromosomes 1 and 9. Identification of these two chromosomal regions should facilitate the identification of genes involved in mammalian oocyte maturation and the control of meiosis.

  13. Homologous SV40 RNA trans-splicing

    PubMed Central

    Eul, Joachim; Patzel, Volker

    2013-01-01

    Simian Virus 40 (SV40) is a polyomavirus found in both monkeys and humans, which causes cancer in some animal models. In humans, SV40 has been reported to be associated with cancers but causality has not been proven yet. The transforming activity of SV40 is mainly due to its 94-kD large T antigen, which binds to the retinoblastoma (pRb) and p53 tumor suppressor proteins, and thereby perturbs their functions. Here we describe a 100 kD super T antigen harboring a duplication of the pRB binding domain that was associated with unusual high cell transformation activity and that was generated by a novel mechanism involving homologous RNA trans-splicing of SV40 early transcripts in transformed rodent cells. Enhanced trans-splice activity was observed in clones carrying a single point mutation in the large T antigen 5′ donor splice site (ss). This mutation impaired cis-splicing in favor of an alternative trans-splice reaction via a cryptic 5′ss within a second cis-spliced SV40 pre-mRNA molecule and enabled detectable gene expression. Next to the cryptic 5′ss we identified additional trans-splice helper functions, including putative dimerization domains and a splice enhancer sequence. Our findings suggest RNA trans-splicing as a SV40-intrinsic mechanism that supports the diversification of viral RNA and phenotypes. PMID:24178438

  14. Evidence of SV40 infections in hospitalized children

    NASA Technical Reports Server (NTRS)

    Butel, J. S.; Jafar, S.; Wong, C.; Arrington, A. S.; Opekun, A. R.; Finegold, M. J.; Adam, E.

    1999-01-01

    Simian virus 40 (SV40) is known to have contaminated poliovirus vaccines used between 1955 and 1963. Accumulating reports have described the presence of SV40 DNA in human tumors and normal tissues, although the significance of human infections by SV40 is unknown. We investigated whether unselected hospitalized children had evidence of SV40 infections and whether any clinical correlations were apparent. Serum samples were examined for SV40 neutralizing antibody using a specific plaque reduction test; of 337 samples tested, 20 (5.9%) had antibody to SV40. Seropositivity increased with age and was significantly associated with kidney transplants (6 of 15 [40%] positive, P < .001). Many of the antibody-positive patients had impaired immune systems. Molecular assays (polymerase chain reaction and DNA sequence analysis) on archival tissue specimens confirmed the presence of SV40 DNA in 4 of the antibody-positive patients. This study, using 2 independent assays, shows the presence of SV40 infections in children born after 1980. We conclude that SV40 causes natural infections in humans.

  15. Immunization with SV40-transformed cells yields mainly MHC-restricted monoclonal antibodies

    PubMed Central

    1986-01-01

    Recognition of antigens on cell surfaces only in the context of the MHC- encoded alloantigens of the presenting cell (self + X) has classically been considered the province of T cells. However, evidence from several sources has indicated that B cells and antibodies can exhibit self + X- restricted recognition as well. This report concerns the mAb response to SV40-transformed H-2b fibroblast cell lines. The specificities of the antibodies obtained have been analyzed for binding to a panel of SV40-transformed H-2-syngeneic, H-2-allogeneic, and H-2b mutant fibroblast cell lines, as well as cell lines not bearing cell surface SV40 transformation-associated antigens. A large proportion of primary C57BL/6 (71%) and BALB/c (68%) splenic B cells responding to in vitro stimulation with SV40-transformed H-2b cells recognize cell surface antigens associated with SV40 transformation only when coexpressed with MHC antigens of the immunizing cell, particularly the Kb molecule, on transformed cells. To extensively define the nature of antigen recognition by these antibodies, we have generated and characterized nine hybridoma antibodies specific for SV40-transformed H-2-syngeneic cell lines. Seven of these hybridoma antibodies recognize SV40- associated transformation antigens in the context of H-2b molecules. Six of these are restricted by the Kb molecule and discriminate among a panel of SV40-transformed Kb mutant cell lines, thus confirming the participation of class I MHC-encoded molecules in the recognition by B cells of cell surface antigens. PMID:3014034

  16. 23 CFR 924.5 - Policy.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... resulting from crashes on all public roads. (b) Under 23 U.S.C. 148(a)(3), a variety of highway safety... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HIGHWAY SAFETY HIGHWAY SAFETY IMPROVEMENT PROGRAM § 924.5 Policy. (a... advance safety. States shall fund safety projects or activities that are most likely to reduce the number...

  17. Synthetic, Infrared, 1H and 13C NMR Spectral Studies on N-(2-/3-Substituted Phenyl)-4-Substituted Benzenesulphonamides, 4-X'C6H4SO2NH(2-/3-XC6H4), where X' = H, CH3, C2H5, F, Cl or Br, and X = CH3 or Cl

    NASA Astrophysics Data System (ADS)

    Gowda, B. Thimme; Shetty, Mahesha; Jayalakshmi, K. L.

    2005-02-01

    Twenty three N-(2-/3-substituted phenyl)-4-substituted benzenesulphonamides of the general formula, 4-X'C6H4SO2NH(2-/3-XC6H4), where X' = H, CH3, C2H5, F, Cl or Br and X = CH3 or Cl have been prepared and characterized, and their infrared spectra in the solid state, 1H and 13C NMR spectra in solution were studied. The N-H stretching vibrations, νN-H, absorb in the range 3285 - 3199 cm-1, while the asymmetric and symmetric SO2 vibrations vary in the ranges 1376 - 1309 cm-1 and 1177 - 1148 cm-1, respectively. The S-N and C-N stretching vibrations absorb in the ranges 945 - 893 cm-1 and 1304 - 1168 cm-1, respectively. The compounds do not exhibit particular trends in the variation of these frequencies on substitution either at ortho or meta positions with either a methyl group or Cl. The observed 1H and 13C chemical shifts of are assigned to protons and carbons of the two benzene rings. Incremental shifts of the ring protons and carbons due to -SO2NH(2-/3-XC6H4) groups in C6H5SO2NH(2-/3-XC6H4), and 4- X'C6H4SO2- and 4-X'C6H4SO2NH- groups in 4-X'C6H4SO2NH(C6H5) are computed and employed to calculate the chemical shifts of the ring protons and carbons in the substituted compounds, 4-X'C6H4SO2NH(2-/3-XC6H4). The computed values agree well with the observed chemical shifts.

  18. Fibroblast growth factor 23 in oncogenic osteomalacia and X-linked hypophosphatemia.

    PubMed

    Jonsson, Kenneth B; Zahradnik, Richard; Larsson, Tobias; White, Kenneth E; Sugimoto, Toshitsugu; Imanishi, Yasuo; Yamamoto, Takehisa; Hampson, Geeta; Koshiyama, Hiroyuki; Ljunggren, Osten; Oba, Koichi; Yang, In Myung; Miyauchi, Akimitsu; Econs, Michael J; Lavigne, Jeffrey; Jüppner, Harald

    2003-04-24

    Mutations in fibroblast growth factor 23 (FGF-23) cause autosomal dominant hypophosphatemic rickets. Clinical and laboratory findings in this disorder are similar to those in oncogenic osteomalacia, in which tumors abundantly express FGF-23 messenger RNA, and to those in X-linked hypophosphatemia, which is caused by inactivating mutations in a phosphate-regulating endopeptidase called PHEX. Recombinant FGF-23 induces phosphaturia and hypophosphatemia in vivo, suggesting that it has a role in phosphate regulation. To determine whether FGF-23 circulates in healthy persons and whether it is elevated in those with oncogenic osteomalacia or X-linked hypophosphatemia, an immunometric assay was developed to measure it. Using affinity-purified, polyclonal antibodies against [Tyr223]FGF-23(206-222)amide and [Tyr224]FGF-23(225-244)amide, we developed a two-site enzyme-linked immunosorbent assay that detects equivalently recombinant human FGF-23, the mutant form in which glutamine is substituted for arginine at position 179 (R179Q), and synthetic human FGF-23(207-244)amide. Plasma or serum samples from 147 healthy adults (mean [+/-SD] age, 48.4+/-19.6 years) and 26 healthy children (mean age, 10.9+/-5.5 years) and from 17 patients with oncogenic osteomalacia (mean age, 43.0+/-13.3 years) and 21 patients with X-linked hypophosphatemia (mean age, 34.9+/-17.2 years) were studied. Mean FGF-23 concentrations in the healthy adults and children were 55+/-50 and 69+/-36 reference units (RU) per milliliter, respectively. Four patients with oncogenic osteomalacia had concentrations ranging from 426 to 7970 RU per milliliter, which normalized after tumor resection. FGF-23 concentrations were 481+/-528 RU per milliliter in those with suspected oncogenic osteomalacia and 353+/-510 RU per milliliter (range, 31 to 2335) in those with X-linked hypophosphatemia. FGF-23 is readily detectable in the plasma or serum of healthy persons and can be markedly elevated in those with oncogenic

  19. One-step simultaneous detection of Ureaplasma parvum and genotypes SV1, SV3 and SV6 from clinical samples using PlexPCR technology.

    PubMed

    Payne, M S; Furfaro, L L; Tucker, R; Tan, L Y; Mokany, E

    2017-08-01

    Ureaplasma spp. are associated with preterm birth. In recent times, it has become apparent that Ureaplasma parvum, but not Ureaplasma urealyticum, is of most relevance. We recently demonstrated this in Australian pregnant women and using high-resolution melt (HRM) PCR, further showed that U. parvum genotype SV6 was of particular significance. However, our assay was unable to identify multiple genotypes in the same sample, required a separate species-level qPCR for low titre samples and was not ideal for diagnostic laboratories due to the nature of HRM PCR result interpretation. Consequently, our current study developed a novel, one-step PlexPCR assay capable of detecting U. parvum and genotypes SV1, SV3 and SV6 in a single reaction directly from clinical samples. We then validated this using vaginal swab DNA from our Australian cohort of pregnant women. The PlexPCR was highly sensitive, detecting all targets to between 0.4 × 10 -5  ng DNA (SV3) and 0.4 × 10 -6  ng DNA (U. parvum, SV1 and SV6). Compared to our HRM PCR, the PlexPCR defined genotype distribution in all seven cases previously reported as 'mixed', and detected another eight cases where multiple genotypes (two) were present in samples previously reported as single genotypes using HRM PCR. Ureaplasma spp. have been associated with prematurity for decades, however, only a minority of studies have examined this beyond the genus level. In those that have, Ureaplasma parvum has been strongly associated with preterm birth. We recently demonstrated this in Australian women and further showed that U. parvum genotype SV6 was of particular significance. Our PlexPCR assay allows rapid detection and concurrent genotyping of U. parvum in clinical samples and may be of particular interest to obstetricians, particularly those caring for women at a high risk of preterm birth, and any other disease phenotypes where U. parvum is of interest. © 2017 The Authors. Letters in Applied Microbiology published by John

  20. Crystal structure and magnetic properties of high-oxygen pressure annealed Sr{sub 1-x}La{sub x}Co{sub 0.5}Fe{sub 0.5}O{sub 3-{delta}} (0{<=}x{<=}0.5)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Swierczek, Konrad; Materials Science Division, Argonne National Laboratory, Argonne, IL 60439; Dabrowski, Bogdan

    2009-02-15

    Structural and magnetic studies are presented for the perovskite type Sr{sub 1-x}La{sub x}Co{sub 0.5}Fe{sub 0.5}O{sub 3-{delta}} (0{<=}x{<=}0.5) materials annealed under moderately high-oxygen pressures of {approx}200 atm. A detailed analysis of the room temperature neutron time-of-flight diffraction data reveals that the crystal structure of the sample SrCo{sub 0.5}Fe{sub 0.5}O{sub 2.89(1)}, previously described as vacancy-disordered cubic, is similar to the formerly reported, oxygen-vacancy ordered Sr{sub 8}Fe{sub 8}O{sub 23} compound, i.e. Sr{sub 8}Co{sub 4}Fe{sub 4}O{sub 23} is tetragonal with the I4/mmm symmetry. With an increase of the La content the studied materials become nearly oxygen stoichiometric and a lowering of the crystal symmetrymore » is observed from cubic Pm3-barm (x=0.1 and 0.2) to tetragonal I4/mcm (x=0.3 and 0.4), and finally to monoclinic I12/c1 (x=0.5). Low-temperature structural and magnetic measurements show a ferromagnetic ordering with the maximum Curie temperature near 290 K at x=0.2. - Graphical Abstract: Room temperature Rietveld refinement profile using I4/mmm space group for the oxygen vacancy ordered SrCo{sub 0.5}Fe{sub 0.5}O{sub 2.89} (Sr{sub 8}Co{sub 4}Fe{sub 4}O{sub 23}). Top tick-marks denote allowed reflections in I4/mmm, bottom one emphasize the possibility of inexact indexing using Pm3-barm symmetry. Previous reports indicate that similar ordering is common for SrCo{sub 1-x}Fe{sub x}O{sub 3-{delta}} compounds possibly hindering their applications.« less

  1. Simian virus 40 (SV40)-like DNA sequences not detectable in finnish mesothelioma patients not exposed to SV40-contaminated polio vaccines.

    PubMed

    Hirvonen, A; Mattson, K; Karjalainen, A; Ollikainen, T; Tammilehto, L; Hovi, T; Vainio, H; Pass, H I; Di Resta, I; Carbone, M; Linnainmaa, K

    1999-10-01

    Occupational asbestos exposure can be demonstrated in 80% of mesothelioma cases. A possible role of simian virus 40 (SV40) in the etiology of mesothelioma was raised because several studies reported the presence and expression of SV40-like DNA sequences in human mesotheliomas. It is also known that expression of SV40 large T antigen inhibits cellular Rb and p53. This suggests that SV40 might render infected cells more susceptible to asbestos carcinogenicity. The SV40-like sequences are suggested to have arisen from contaminated polio vaccines. Millions of people in the United States and most European countries were inoculated with SV40-contaminated polio vaccine in 1955-1963. However, in Finland, where polio vaccination started in 1957, no SV40-contaminated vaccine was used. We used a polymerase chain reaction-based method to test for the presence of SV40-like sequences in DNA extracted from the frozen tumor tissues of 49 Finnish mesothelioma patients, most of whom had been occupationally exposed to asbestos. All of the Finnish tumor tissues tested negative for SV40-like sequences. The results suggest that the SV40-like sequences detected in mesothelioma tissue in some previous studies may indeed originate from SV40-contaminated polio vaccines. It is a matter of speculation whether the absence of SV40 infection has contributed to the relatively low incidence of mesothelioma in Finland (1/10(5) in 1990-1995). Copyright 1999 Wiley-Liss, Inc.

  2. Cytopathic effect of Human cosavirus (HCoSV) on primary cell cultures of human embryonic lung MRC5.

    PubMed

    Rezig, Dorra; Touzi, Henda; Meddeb, Zina; Triki, Henda

    2014-10-01

    Human cosaviruses (HCoSVs) are newly discovered viruses in Picornaviridae family. Until now, most published studies reported HCoSV detection using molecular techniques and genetic characterization of the virus. Nevertheless, no laboratory has yet reported the replication of these viruses in cultured cell lines. In the present work, the propagation of HCoSV strains isolated from human fecal specimens in MRC5 cell line and their induced cytopathic effects (CPE) was studied. The first sign of virus growth was observed 24-48h after inoculation. The cells rounded up and clumped together rapidly; empty areas became visible and, on the third day of CPE, a remarkable decrease in live cells was observed. This represents the first report on in vitro model of HCoSV replication which opens up opportunities for future investigations of these new viruses. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. Ba0.06(Na,Bi)0.94Ti1-x(Ni1/3Nb2/3)xO3 ceramics: X-ray diffraction and infrared spectroscopy studies

    NASA Astrophysics Data System (ADS)

    Mishra, R. K.; Prasad, Ashutosh; Chandra, K. P.; Prasad, K.

    2018-05-01

    Non-lead ceramic samples of Ba0.06(Na0.5Bi0.5)0.94Ti1-x(Ni1/3Nb2/3)xO3; 0 ≤ x ≤ 1.0 were prepared by standard high temperature ceramic synthesis method. Rietveld refinements of X-ray diffraction data of these ceramics were carried out using FullProf software and determined their crystal symmetry, space group and unit cell dimensions. Rietveld refinement revealed that Ba0.06(Na0.5Bi0.5)0.94TiO3 has a monoclinic structure with space group P4/m while B0.06(Na0.5Bi0.5)0.94(Ni1/3Nb2/3)O3 has tetragonal (pseudo-cubic) structure with space group P4/mmm. Partial replacement of Ti4+ ion by pseudo-cation (Ni1/33 +Nb2/3 5 +) 4 + resulted in the change of unit cell structure from monoclinic to tetragonal. SEM studies were carried out in order to access the quality of the prepared ceramics which showed a change in grain sizes with the increase of (Ni1/33 +Nb2/3 5 +) 4 + content. FTIR spectra confirmed the formation of perovskite type solid solutions.

  4. 28 CFR 524.23 - Program reviews.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Program reviews. 524.23 Section 524.23... TRANSFER CLASSIFICATION OF INMATES Youth Corrections Act (YCA) Programs § 524.23 Program reviews. Staff shall conduct periodic reviews of the inmate's program plan and shall modify the plan in accordance with...

  5. 7 CFR 550.23 - Program income.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Program income. 550.23 Section 550.23 Agriculture... Financial Management § 550.23 Program income. (a) REE Agencies shall apply the standards set forth in this section in requiring Cooperator organizations to account for program income related to projects financed...

  6. 49 CFR 23.7 - Program reviews.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Program reviews. 23.7 Section 23.7 Transportation... CONCESSIONS General § 23.7 Program reviews. In 2010, and thereafter at the discretion of the Secretary, the Department will initiate a review of the ACDBE program to determine what, if any, modifications should be...

  7. Levetiracetam Reverses Synaptic Deficits Produced by Overexpression of SV2A

    PubMed Central

    Yao, Jia; Bleckert, Adam; Hill, Jessica; Bajjalieh, Sandra M.

    2011-01-01

    Levetiracetam is an FDA-approved drug used to treat epilepsy and other disorders of the nervous system. Although it is known that levetiracetam binds the synaptic vesicle protein SV2A, how drug binding affects synaptic functioning remains unknown. Here we report that levetiracetam reverses the effects of excess SV2A in autaptic hippocampal neurons. Expression of an SV2A-EGFP fusion protein produced a ∼1.5-fold increase in synaptic levels of SV2, and resulted in reduced synaptic release probability. The overexpression phenotype parallels that seen in neurons from SV2 knockout mice, which experience severe seizures. Overexpression of SV2A also increased synaptic levels of the calcium-sensor protein synaptotagmin, an SV2-binding protein whose stability and trafficking are regulated by SV2. Treatment with levetiracetam rescued normal neurotransmission and restored normal levels of SV2 and synaptotagmin at the synapse. These results indicate that changes in SV2 expression in either direction impact neurotransmission, and suggest that levetiracetam may modulate SV2 protein interactions. PMID:22220214

  8. ER Operations Installation of Three FLUTe Soil-Vapor Monitoring Wells (MWL-SV03 MWL-SV04 and MWL-SV05) at the Mixed Waste Landfill.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Copland, John Robin

    2014-09-01

    This installation report describes the May through July 2014 drilling activities performed for the installation of three multi-port soil-vapor monitoring wells (MWL-SV03, MWL-SV04, and MWL-SV05) at the Mixed Waste Landfill (MWL), which is located at Sandia National Laboratories, New Mexico (SNL/NM). SNL/NM is managed and operated by Sandia Corporation (Sandia), a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy (DOE)/National Nuclear Security Administration. The MWL is designated as Solid Waste Management Unit (SWMU) 76 and is located in Technical Area (TA) III (Figure 1-1). The locations of the three soil-vapor monitoring wells (MWL-SV03, MWL-SV04, andmore » MWL-SV05) are shown in Figure 1-2« less

  9. Competitive antagonism of recombinant P2X(2/3) receptors by 2', 3'-O-(2,4,6-trinitrophenyl) adenosine 5'-triphosphate (TNP-ATP).

    PubMed

    Burgard, E C; Niforatos, W; van Biesen, T; Lynch, K J; Kage, K L; Touma, E; Kowaluk, E A; Jarvis, M F

    2000-12-01

    TNP-ATP has become widely recognized as a potent and selective P2X receptor antagonist, and is currently being used to discriminate between subtypes of P2X receptors in a variety of tissues. We have investigated the ability of TNP-ATP to inhibit alpha,beta-methylene ATP (alpha,beta-meATP)-evoked responses in 1321N1 human astrocytoma cells expressing recombinant rat or human P2X(2/3) receptors. Pharmacological responses were measured using electrophysiological and calcium imaging techniques. TNP-ATP was a potent inhibitor of P2X(2/3) receptors, blocking both rat and human receptors with IC(50) values of 3 to 6 nM. In competition studies, 10 to 1000 microM alpha,beta-meATP was able to overcome TNP-ATP inhibition. Schild analysis revealed that TNP-ATP was a competitive antagonist with pA(2) values of -8.7 and -8.2. Inhibition of P2X(2/3) receptors by TNP-ATP was rapid in onset, reversible, and did not display use dependence. Although the onset kinetics of inhibition were concentration-dependent, the TNP-ATP off-kinetics were concentration-independent and relatively slow. Full recovery from TNP-ATP inhibition did not occur until >/=5 s after removal of the antagonist. Because of the slow off-kinetics of TNP-ATP, full competition with alpha,beta-meATP for receptor occupancy could be seen only after both ligands had reached a steady-state condition. It is proposed that the slowly desensitizing P2X(2/3) receptor allowed this competitive interaction to be observed over time, whereas the rapid desensitization of other P2X receptors (P2X(3)) may mask the detection of competitive inhibition by TNP-ATP.

  10. Subtype-specific regulation of P2X3 and P2X2/3 receptors by phosphoinositides in peripheral nociceptors

    PubMed Central

    Mo, Gary; Bernier, Louis-Philippe; Zhao, Qi; Chabot-Doré, Anne-Julie; Ase, Ariel R; Logothetis, Diomedes; Cao, Chang-Qing; Séguéla, Philippe

    2009-01-01

    Background P2X3 and P2X2/3 purinergic receptor-channels, expressed in primary sensory neurons that mediate nociception, have been implicated in neuropathic and inflammatory pain responses. The phospholipids phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3) are involved in functional modulation of several types of ion channels. We report here evidence that these phospholipids are able to modulate the function of homomeric P2X3 and heteromeric P2X2/3 purinoceptors expressed in dorsal root ganglion (DRG) nociceptors and in heterologous expression systems. Results In dissociated rat DRG neurons, incubation with the PI3K/PI4K inhibitor wortmannin at 35 μM induced a dramatic decrease in the amplitude of ATP- or α,β-meATP-evoked P2X3 currents, while incubation with 100 nM wortmannin (selective PI3K inhibition) produced no significant effect. Intracellular application of PIP2 was able to fully reverse the inhibition of P2X3 currents induced by wortmannin. In Xenopus oocytes and in HEK293 cells expressing recombinant P2X3, 35 μM wortmannin incubation induced a significant decrease in the rate of receptor recovery. Native and recombinant P2X2/3 receptor-mediated currents were inhibited by incubation with wortmannin both at 35 μM and 100 nM. The decrease of P2X2/3 current amplitude induced by wortmannin could be partially reversed by application of PIP2 or PIP3, indicating a sensitivity to both phosphoinositides in DRG neurons and Xenopus oocytes. Using a lipid binding assay, we demonstrate that the C-terminus of the P2X2 subunit binds directly to PIP2, PIP3 and other phosphoinositides. In contrast, no direct binding was detected between the C-terminus of P2X3 subunit and phosphoinositides. Conclusion Our findings indicate a functional regulation of homomeric P2X3 and heteromeric P2X2/3 ATP receptors by phosphoinositides in the plasma membrane of DRG nociceptors, based on subtype-specific mechanisms of direct and indirect

  11. Random integration of SV40 in SV40-transformed, immortalized human fibroblasts.

    PubMed

    Hara, H; Kaji, H

    1987-02-01

    We have studied the relationship between immortalization of SV40-transformed human embryonic fibroblasts and their SV40 integration sites. From several independently transformed cell pools, we have isolated clones which do not harbor unintegrated SV40 DNA. We have analysed whole-cell DNA from these clones, using the Southern blot method. Our results suggest that no specific integration sites in the cellular genome exist which are a prerequisite for the immortalization process. Although some integration sites were found to be predominant in pre-crisis clones, they could not be detected in the post-crisis clones. This suggests that none of these predominating sites is selected for during the crisis period.

  12. Comets C/2003 X5-X11 and Y2-Y10 (SOHO)

    NASA Astrophysics Data System (ADS)

    Battams, K.; Boschat, M.; Zhou, X.-M.; Hoffman, T.; Leprette, X.; Matson, R.; Kracht, R.; Sachs, J.; Marsden, B. G.; Kisala, R.

    2004-06-01

    Further to IAUC 8356, K. Battams reports measurements for additional Kreutz sungrazing comets found on SOHO website C2 images by M. Boschat (C/2003 X5, X7, Y5, Y8), X.-m. Zhou (C/2003 X6, X9, X11), T. Hoffman (C/2003 X8), X. Leprette (C/2003 X10, Y2), R. Matson (2003 Y3, Y9, Y10), R. Kracht (C/2003 Y4, Y7), and J. Sachs (C/2003 Y6). C/2003 Y6 and Y7 were also visible on C3 images. The reductions by B. G. Marsden (and by R. Kisala for C/2003 Y8, Y9, Y10) and orbital elements by Marsden appear on the MPECs cited below. Comet 2003 UT R.A. (2000) Decl. MPEC C/2003 X5 Dec. 4.896 16 46.9 -24 12 2004-L24 C/2003 X6 6.396 16 52.2 -24 20 2004-L24 C/2003 X7 7.829 16 58.6 -24 27 2004-L24 C/2003 X8 8.246 17 00.9 -24 31 2004-L24 C/2003 X9 8.621 17 02.2 -24 34 2004-L24 C/2003 X10 11.188 17 14.5 -24 41 2004-L25 C/2003 X11 13.588 17 25.7 -24 57 2004-L25 C/2003 Y2 19.621 17 53.6 -25 08 2004-L25 C/2003 Y3 19.979 17 55.0 -25 02 2004-L25 C/2003 Y4 20.729 17 58.8 -25 14 2004-L25 C/2003 Y5 22.771 18 08.5 -25 08 2004-L25 C/2003 Y6 23.571 18 14.3 -27 22 2004-L26 C/2003 Y7 24.638 18 20.0 -27 44 2004-L26 C/2003 Y8 25.288 18 19.6 -25 00 2004-L67 C/2003 Y9 25.479 18 20.7 -24 46 2004-L67 C/2003 Y10 26.064 18 23.5 -24 50 2004-L67

  13. Can We Perform CT of the Appendix with Less Than 1 mSv? A De-escalating Dose-simulation Study.

    PubMed

    Park, Ji Hoon; Jeon, Jong-June; Lee, Sung Soo; Dhanantwari, Amar C; Sim, Ji Ye; Kim, Hae Young; Lee, Kyoung Ho

    2018-05-01

    To systematically explore the lowest reasonably achievable radiation dose for appendiceal CT using an iterative reconstruction (IR) in young adults. We prospectively included 30 patients who underwent 2.0-mSv CT for suspected appendicitis. From the helical projection data, 1.5-, 1.0- and 0.5-mSv CTs were generated using a low-dose simulation tool and the knowledge-based IR. We performed step-wise non-inferiority tests sequentially comparing 2.0-mSv CT with each of 1.5-, 1.0- and 0.5-mSv CT, with a predetermined non-inferiority margin of 0.06. The primary end point was the pooled area under the receiver-operating-characteristic curve (AUC) for three abdominal and three non-abdominal radiologists. For the abdominal radiologists, the non-inferiorities of 1.5-, 1.0- and 0.5-mSv CT to 2.0-mSv CT were sequentially accepted [pooled AUC difference: 2.0 vs. 0.5 mSv, 0.017 (95% CI: -0.016, 0.050)]. For the non-abdominal radiologists, the non-inferiorities of 1.5- and 1.0-mSv CT were accepted; however, the non-inferiority of 0.5-mSv CT could not be proved [pooled AUC difference: 2.0 vs. 1.0 mSv, -0.017 (-0.070, 0.035) and 2.0 vs. 0.5 mSv, 0.045 (-0.071, 0.161)]. The 1.0-mSv appendiceal CT was non-inferior to 2.0-mSv CT in terms of diagnostic performance for both abdominal and non-abdominal radiologists; 0.5-mSv appendiceal CT was non-inferior only for abdominal radiologists. • For both abdominal and non-abdominal radiologists, 1.0-mSv appendiceal CT could be feasible. • The 0.5-mSv CT was non-inferior to 2.0-mSv CT only for expert abdominal radiologists. • Reader experience is an important factor affecting diagnostic impairment by low-dose CT.

  14. 49 CFR 23.23 - What administrative provisions must be in a recipient's ACDBE program?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... recipient's ACDBE program? 23.23 Section 23.23 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS ACDBE Programs § 23.23 What... provided in part 26, §§ 26.23, 26.25, and 26.31, as well as certification of ACDBEs as provided by Subpart...

  15. 49 CFR 23.23 - What administrative provisions must be in a recipient's ACDBE program?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... recipient's ACDBE program? 23.23 Section 23.23 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS ACDBE Programs § 23.23 What... provided in part 26, §§ 26.23, 26.25, and 26.31, as well as certification of ACDBEs as provided by Subpart...

  16. 49 CFR 23.23 - What administrative provisions must be in a recipient's ACDBE program?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... recipient's ACDBE program? 23.23 Section 23.23 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS ACDBE Programs § 23.23 What... provided in part 26, §§ 26.23, 26.25, and 26.31, as well as certification of ACDBEs as provided by Subpart...

  17. 49 CFR 23.23 - What administrative provisions must be in a recipient's ACDBE program?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... recipient's ACDBE program? 23.23 Section 23.23 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS ACDBE Programs § 23.23 What... provided in part 26, §§ 26.23, 26.25, and 26.31, as well as certification of ACDBEs as provided by Subpart...

  18. 49 CFR 23.23 - What administrative provisions must be in a recipient's ACDBE program?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... recipient's ACDBE program? 23.23 Section 23.23 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS ACDBE Programs § 23.23 What... provided in part 26, §§ 26.23, 26.25, and 26.31, as well as certification of ACDBEs as provided by Subpart...

  19. Solid state fermentation and production of rifamycin SV using Amycolatopsis mediterranei.

    PubMed

    Nagavalli, M; Ponamgi, S P D; Girijashankar, V; Venkateswar Rao, L

    2015-01-01

    Production of Rifamycin SV from cheaper agro-industrial by-products using mutant strain of Amycolatopsis mediterranei OVA5-E7 in solid state fermentation (SSF) was optimized. Among the agro-based substrates used, ragi bran was found suitable for maximizing the yield of Rifamycin SV (1310 mg 100 g(-1) ds). The yield can be further enhanced to 19·7 g Kg(-1) of dry substrate by supplementing the substrate with deoiled cotton cake (10% w/w) using optimized fermentation parameters such as maintaining 80% moisture, pH 7·0, 30°C incubation temperature, inoculum 25% v/w and carrying the solid state fermenting for 9 days. Manipulating these seven specifications, the end product yield achieved in our experimentation was 20 g of Rifamycin SV Kg(-1) ds. Eventually, an overall 5-fold improvement in Rifamycin SV production was achieved. Antibiotics such as rifamycin are broad-spectrum antimicrobial drugs used in large-scale worldwide as human medicine towards controlling diseases. Amycolatopsis mediterranei strain which produces this antibiotic was earlier used in submerged fermentation yielded lower amounts of rifamycin. By employing cheaper agro-industrial by-products, we produced upto 20 g rifamycin SV per Kg dry substrate used under optimized solid state fermentation conditions. Keeping in view, the role of rifamycin in meeting the medical demands of world's increasing population; we successfully used an improved strain on cheaper substrates with optimized fermentation parameters and achieved a 5-fold improvement in rifamycin SV production. © 2014 The Society for Applied Microbiology.

  20. Variable frequency of polyomavirus SV40 and herpesvirus EBV in lymphomas from two different urban population groups in Houston, TX.

    PubMed

    Toracchio, Sonia; Kozinetz, Claudia A; Killen, Deanna E; Sheehan, Andrea M; Banez, Eugenio I; Ittmann, Michael M; Sroller, Vojtech; Butel, Janet S

    2009-10-01

    Studies have reported differing frequencies of detection of polyomavirus simian virus 40 (SV40) in association with human lymphomas. We addressed the hypothesis that SV40 positivity in lymphomas can vary among sampled populations. Archival paraffin-embedded lymphoma specimens (n=171) from patients at two urban hospitals in Houston, TX, USA, were analyzed following a cross-sectional study design. Extracted DNAs were characterized by quantitative polymerase chain reaction for the cellular RNase P gene and for SV40 and herpesvirus Epstein-Barr virus (EBV) sequences. Patient characteristics of the two study populations differed significantly whereas the classification of tumor types studied did not. SV40 DNA was detected more frequently in lymphomas from the public hospital population (10/44, 23%) than in lymphomas from the veterans' hospital (VAMC) (4/127, 3%; P<0.0001). EBV detection in lymphomas also differed between the two groups (17/44, 39% vs. 23/127, 18%; P=0.01). SV40 positivity was associated with a younger age category of VAMC lymphoma patients (P=0.02). Expression of T-antigen was detected by immunohistochemistry in half of lymphomas that contained SV40 DNA. Variation was observed in the quality and quantity of DNA recovered from paraffin-embedded specimens, but there was no difference in recoveries of DNA from samples from the two hospitals. This study demonstrated that, in a direct comparison, the prevalence of SV40 DNA in lymphomas can differ significantly between groups with different demographic distributions.

  1. 23 CFR 140.603 - Programs.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Programs. 140.603 Section 140.603 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PAYMENT PROCEDURES REIMBURSEMENT Reimbursement for Bond Issue Projects § 140.603 Programs. Programs covering projects to be financed from the proceeds of...

  2. 23 CFR 140.603 - Programs.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Programs. 140.603 Section 140.603 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PAYMENT PROCEDURES REIMBURSEMENT Reimbursement for Bond Issue Projects § 140.603 Programs. Programs covering projects to be financed from the proceeds of...

  3. 23 CFR 140.603 - Programs.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Programs. 140.603 Section 140.603 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PAYMENT PROCEDURES REIMBURSEMENT Reimbursement for Bond Issue Projects § 140.603 Programs. Programs covering projects to be financed from the proceeds of...

  4. X-31 Loaded in C-5 Cargo Bay

    NASA Technical Reports Server (NTRS)

    1995-01-01

    The X-31 Enhanced Fighter Maneuverability Technology Demonstrator Aircraft, based at the NASA Dryden Flight Research Center, Edwards Air Force Base, California, is secured inside the fuselage of an Air Force Reserve C-5 transport. The C-5 was used to ferry the X-31 from Europe back to Edwards, after being flown in the Paris Air Show in June 1995. The X-31's right wing, removed so the aircraft could fit inside the C-5, is in the shipping container in the foreground. At the air show, the X-31 demonstrated the value of using thrust vectoring (directing engine exhaust flow) coupled with advanced flight control systems to provide controlled flight at very high angles of attack. The X-31 Enhanced Fighter Maneuverability (EFM) demonstrator flew at the Ames- Dryden Flight Research Facility, Edwards, California (redesignated the Dryden Flight Research Center in 1994) from February 1992 until 1995 and before that at the Air Force's Plant 42 in Palmdale, California. The goal of the project was to provide design information for the next generation of highly maneuverable fighter aircraft. This program demonstrated the value of using thrust vectoring (directing engine exhaust flow) coupled with an advanced flight control system to provide controlled flight to very high angles of attack. The result was a significant advantage over most conventional fighters in close-in combat situations. The X-31 flight program focused on agile flight within the post-stall regime, producing technical data to give aircraft designers a better understanding of aerodynamics, effectiveness of flight controls and thrust vectoring, and airflow phenomena at high angles of attack. Stall is a condition of an airplane or an airfoil in which lift decreases and drag increases due to the separation of airflow. Thrust vectoring compensates for the loss of control through normal aerodynamic surfaces that occurs during a stall. Post-stall refers to flying beyond the normal stall angle of attack, which in the X-31

  5. Development of β Type Ti23Mo-45S5 Bioglass Nanocomposites for Dental Applications

    PubMed Central

    Jurczyk, Karolina; Miklaszewski, Andrzej; Jurczyk, Mieczyslawa U.; Jurczyk, Mieczyslaw

    2015-01-01

    Titanium β-type alloys attract attention as biomaterials for dental applications. The aim of this work was the synthesis of nanostructured β type Ti23Mo-x wt % 45S5 Bioglass (x = 0, 3 and 10) composites by mechanical alloying and powder metallurgy methods and their characterization. The crystallization of the amorphous material upon annealing led to the formation of a nanostructured β type Ti23Mo alloy with a grain size of approximately 40 nm. With the increase of the 45S5 Bioglass contents in Ti23Mo, nanocomposite increase of the α-phase is noticeable. The electrochemical treatment in phosphoric acid electrolyte resulted in a porous surface, followed by bioactive ceramic Ca-P deposition. Corrosion resistance potentiodynamic testing in Ringer solution at 37 °C showed a positive effect of porosity and Ca-P deposition on nanostructured Ti23Mo 3 wt % 45S5 Bioglass nanocomposite. The contact angles of glycerol on the nanostructured Ti23Mo alloy were determined and show visible decrease for bulk Ti23Mo 3 wt % 45S5 Bioglass and etched Ti23Mo 3 wt % 45S5 Bioglass nanocomposites. In vitro tests culture of normal human osteoblast cells showed very good cell proliferation, colonization, and multilayering. The present study demonstrated that porous Ti23Mo 3 wt % 45S5 Bioglass nanocomposite is a promising biomaterial for bone tissue engineering. PMID:28793695

  6. Navy CG(X) Cruiser Program: Background for Congress

    DTIC Science & Technology

    2010-09-28

    NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS...Peters, “Navy’s Top Officer Sees Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September 24, 2008) quoted Admiral Gary Roughead...number of CG(X)s could have changed.38 A related question was whether the schedule for procuring CG(X)s was properly aligned with foreign-country

  7. Navy CG(X) Cruiser Program: Background for Congress

    DTIC Science & Technology

    2010-02-26

    5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS...Peters, “Navy’s Top Officer Sees Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September 24, 2008) quoted Admiral Gary Roughead...CG(X)s could change.40 A related question was whether the schedule for procuring CG(X)s was properly aligned with foreign-country ballistic missile

  8. 23 CFR 1313.5 - Requirements for a low fatality rate state.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Requirements for a low fatality rate state. 1313.5 Section 1313.5 Highways NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION INCENTIVE GRANT CRITERIA FOR ALCOHOL-IMPAIRED DRIVING PREVENTION PROGRAMS § 1313.5 Requirements for a low...

  9. Magnetic Properties of the Ferromagnetic Shape Memory Alloys Ni50+xMn27−xGa23 in Magnetic Fields

    PubMed Central

    Sakon, Takuo; Otsuka, Kohei; Matsubayashi, Junpei; Watanabe, Yuushi; Nishihara, Hironori; Sasaki, Kenta; Yamashita, Satoshi; Umetsu, Rie Y.; Nojiri, Hiroyuki; Kanomata, Takeshi

    2014-01-01

    Thermal strain, permeability, and magnetization measurements of the ferromagnetic shape memory alloys Ni50+xMn27−xGa23 (x = 2.0, 2.5, 2.7) were performed. For x = 2.7, in which the martensite transition and the ferromagnetic transition occur at the same temperature, the martensite transition starting temperature TMs shift in magnetic fields around a zero magnetic field was estimated to be dTMs/dB = 1.1 ± 0.2 K/T, thus indicating that magnetic fields influences martensite transition. We discussed the itinerant electron magnetism of x = 2.0 and 2.5. As for x = 2.5, the M4 vs. B/M plot crosses the origin of the coordinate axis at the Curie temperature, and the plot indicates a good linear relation behavior around the Curie temperature. The result is in agreement with the theory by Takahashi, concerning itinerant electron ferromagnets. PMID:28788645

  10. 34 CFR 300.23 - Individualized education program team.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Individualized education program team. 300.23 Section 300.23 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF... education program team. Individualized education program team or IEP Team means a group of individuals...

  11. 34 CFR 300.23 - Individualized education program team.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Individualized education program team. 300.23 Section 300.23 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF... education program team. Individualized education program team or IEP Team means a group of individuals...

  12. 34 CFR 300.23 - Individualized education program team.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Individualized education program team. 300.23 Section 300.23 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF... education program team. Individualized education program team or IEP Team means a group of individuals...

  13. 34 CFR 300.23 - Individualized education program team.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Individualized education program team. 300.23 Section 300.23 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF... education program team. Individualized education program team or IEP Team means a group of individuals...

  14. 23 CFR 1200.24 - Program income.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Program income. 1200.24 Section 1200.24 Highways... Implementation and Management of the Highway Safety Program § 1200.24 Program income. (a) Inclusions. Program income includes income from fees for services performed, from the use or rental of real or personal...

  15. 7 CFR 1469.23 - Program payments.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CONSERVATION SECURITY PROGRAM Contracts and Payments § 1469.23 Program payments. (a) Stewardship component of CSP payments. (1) The conservation stewardship plan... Agriculture Statistics Service (NASS) land rental data, and Conservation Reserve Program (CRP) rental rates...

  16. Production of SV40-derived vectors.

    PubMed

    Strayer, David S; Mitchell, Christine; Maier, Dawn A; Nichols, Carmen N

    2010-06-01

    Recombinant simian virus 40 (rSV40)-derived vectors are particularly useful for gene delivery to bone marrow progenitor cells and their differentiated derivatives, certain types of epithelial cells (e.g., hepatocytes), and central nervous system neurons and microglia. They integrate rapidly into cellular DNA to provide long-term gene expression in vitro and in vivo in both resting and dividing cells. Here we describe a protocol for production and purification of these vectors. These procedures require only packaging cells (e.g., COS-7) and circular vector genome DNA. Amplification involves repeated infection of packaging cells with vector produced by transfection. Cotransfection is not required in any step. Viruses are purified by centrifugation using discontinuous sucrose or cesium chloride (CsCl) gradients and resulting vectors are replication-incompetent and contain no detectable wild-type SV40 revertants. These approaches are simple, give reproducible results, and may be used to generate vectors that are deleted only for large T antigen (Tag), or for all SV40-coding sequences capable of carrying up to 5 kb of foreign DNA. These vectors are best applied to long-term expression of proteins normally encoded by mammalian cells or by viruses that infect mammalian cells, or of untranslated RNAs (e.g., RNA interference). The preparative approaches described facilitate application of these vectors and allow almost any laboratory to exploit their strengths for diverse gene delivery applications.

  17. NBSGSC - a FORTRAN program for quantitative x-ray fluorescence analysis. Technical note (final)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tao, G.Y.; Pella, P.A.; Rousseau, R.M.

    1985-04-01

    A FORTRAN program (NBSGSC) was developed for performing quantitative analysis of bulk specimens by x-ray fluorescence spectrometry. This program corrects for x-ray absorption/enhancement phenomena using the comprehensive alpha coefficient algorithm proposed by Lachance (COLA). NBSGSC is a revision of the program ALPHA and CARECAL originally developed by R.M. Rousseau of the Geological Survey of Canada. Part one of the program (CALCO) performs the calculation of theoretical alpha coefficients, and part two (CALCOMP) computes the composition of the analyte specimens. The analysis of alloys, pressed minerals, and fused specimens can currently be treated by the program. In addition to using measuredmore » x-ray tube spectral distributions, spectra from seven commonly used x-ray tube targets could also be calculated with an NBS algorithm included in the program. NBSGSC is written in FORTRAN IV for a Digital Equipment Corporation (DEC PDP-11/23) minicomputer using RLO2 firm disks and an RSX 11M operating system.« less

  18. Randomized trial of the anti-FGF23 antibody KRN23 in X-linked hypophosphatemia

    PubMed Central

    Carpenter, Thomas O.; Imel, Erik A.; Ruppe, Mary D.; Weber, Thomas J.; Klausner, Mark A.; Wooddell, Margaret M.; Kawakami, Tetsuyoshi; Ito, Takahiro; Zhang, Xiaoping; Humphrey, Jeffrey; Insogna, Karl L.; Peacock, Munro

    2014-01-01

    Background. X-linked hypophosphatemia (XLH) is the most common heritable form of rickets and osteomalacia. XLH-associated mutations in phosphate-regulating endopeptidase (PHEX) result in elevated serum FGF23, decreased renal phosphate reabsorption, and low serum concentrations of phosphate (inorganic phosphorus, Pi) and 1,25-dihydroxyvitamin D [1,25(OH)2D]. KRN23 is a human anti-FGF23 antibody developed as a potential treatment for XLH. Here, we have assessed the safety, tolerability, pharmacokinetics (PK), pharmacodynamics (PD), and immunogenicity of KRN23 following a single i.v. or s.c. dose of KRN23 in adults with XLH. Methods. Thirty-eight XLH patients were randomized to receive a single dose of KRN23 (0.003–0.3 mg/kg i.v. or 0.1–1 mg/kg s.c.) or placebo. PK, PD, immunogenicity, safety, and tolerability were assessed for up to 50 days. Results. KRN23 significantly increased the maximum renal tubular threshold for phosphate reabsorption (TmP/GFR), serum Pi, and 1,25(OH)2D compared with that of placebo (P < 0.01). The maximum serum Pi concentration occurred later following s.c. dosing (8–15 days) compared with that seen with i.v. dosing (0.5–4 days). The effect duration was dose related and persisted longer in patients who received s.c. administration. Changes from baseline in TmP/GFR, serum Pi, and serum 1,25(OH)2D correlated with serum KRN23 concentrations. The mean t1/2 of KRN23 was 8–12 days after i.v. administration and 13–19 days after s.c. administration. Patients did not exhibit increased nephrocalcinosis or develop hypercalciuria, hypercalcemia, anti-KRN23 antibodies, or elevated serum parathyroid hormone (PTH) or creatinine. Conclusion. KRN23 increased TmP/GFR, serum Pi, and serum 1,25(OH)2D. The positive effect of KR23 on serum Pi and its favorable safety profile suggest utility for KRN23 in XLH patients. Trial registration. Clinicaltrials.gov NCT00830674. Funding. Kyowa Hakko Kirin Pharma, Inc. PMID:24569459

  19. Immunization of mice with baculovirus-derived recombinant SV40 large tumour antigen induces protective tumour immunity to a lethal challenge with SV40-transformed cells.

    PubMed Central

    Shearer, M H; Bright, R K; Lanford, R E; Kennedy, R C

    1993-01-01

    In this study, we examined the humoral immune responses and in vivo tumour immunity induced by baculovirus recombinant simian virus 40 (SV40) large tumour antigen (rSV40 T-ag). BALB/c mice immunized with rSV40 T-ag produced antibody responses that recognized SV40 large tumour antigen (T-ag) by ELISA. Analysis of these anti-SV40 T-ag responses indicated that the antibodies recognized epitopes associated with both the carboxy and amino terminus of SV40 T-ag. This pattern of SV40 T-ag epitope recognition was similar to that observed in anti-SV40 T-ag responses induced by inoculation with irradiated SV40-transformed cells. Mice immunized with either rSV40 T-ag or with the inactivated transformed cells were protected from a subsequent in vivo lethal tumour challenge with live SV40-transformed cells. These studies suggest that humoral immune responses induced by rSV40 T-ag are similar in epitope specificity to that induced by inactivated SV40-transformed cells. In addition, recombinant tumour-specific antigens from papovaviruses, such as SV40, can be used to induce tumour immunity which protects from a subsequent lethal tumour challenge. This study may provide insight into the use of recombinant tumour antigens as putative tumour vaccines and in the development of active immunotherapeutic strategies for treating virus-induced cancers. PMID:7679059

  20. 23 CFR 627.7 - VE programs.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false VE programs. 627.7 Section 627.7 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS VALUE ENGINEERING § 627.7 VE programs. (a) The STA shall establish and sustain a VE program under which VE analyses are...

  1. 23 CFR 627.7 - VE programs.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false VE programs. 627.7 Section 627.7 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS VALUE ENGINEERING § 627.7 VE programs. (a) The STA shall establish and sustain a VE program under which VE analyses are...

  2. RXTE and BeppoSAX Observations of MCG-5-23-16: Reflection From Distant Cold Material

    NASA Technical Reports Server (NTRS)

    Mattson, B. J.; Weaver, K. A.

    2003-01-01

    We examine the spectral variability of the Seyfert 1.9 galaxy MCG-5-23-16 using RXTE and BeppoSAX observations spanning 2 years from April 1996 to April 1998. During the first year the X-ray source brightens by a factor of approximately 25% on timescales of days to months. During this time, the reprocessed continuum emission seen with RXTE does not respond measurably to the continuum increase. However, by the end of the second year during the BeppoSAX epoch the X-ray source has faded again. This time, the reprocessed emission has also faded, indicating that the reprocessed flux has responded to the continuum. If these effects are caused by time delays due to the distance between the X-ray source and the reprocessing region, we derive a light crossing time of between approximately 1 light day and approximately 1.5 light years. This corresponds to a distance of 0.001 pc to 0.55 pc, which implies that the reprocessed emission originates between 3 x 10(exp 15) cm and 1.6 x 10(exp l8) cm from the X-ray source. In other words, the reprocessing in MCG-5-23-16 is not dominated by the inner regions of a standard accretion disk.

  3. Structural and relaxor-like dielectric properties of unfilled tungsten bronzes Ba5-5xSm5xTi5xNb10-5xO30

    NASA Astrophysics Data System (ADS)

    Wei, T.; Dong, Z.; Zhao, C. Z.; Guo, Y. Y.; Zhou, Q. J.; Li, Z. P.

    2016-03-01

    New unfilled tetragonal tungsten bronze (TTB) oxides, Ba5-5xSm5xTi5xNb10-5xO30 (BSTN-x), where 0.10 ≤ x ≤ 0.35, have been synthesized in this work. Their crystal structure was determined and analyzed based on Rietveld structural refinement. It is found that single TTB phase can be formed in a particular x range (i.e., 0.15 ≤ x ≤ 0.3) due to the competition interaction between tolerance factor and electronegativity difference. Furthermore, dielectric and ferroelectric results indicate that phase transitions and ferroelectric states are sensitive to x. Referring to the local chemistry, we suggest that the raise of vacancies at the A2-site compared with that of A1-site will intensely depress the normal ferroelectric phase and is in favor of relaxor ferroelectric state. Macroscopically, previous A-site size difference standpoint on fill TTB compounds cannot give a reasonable explanation about the variation of dielectric maximum temperature (Tm) for present BSTN-x compounds. Alternatively, tetragonality (c/a) is adopted which can well describe the variation of Tm in whole x range. In addition, one by one correspondence between tetragonality and electrical features can be found, and the compositions involving high c/a are usually stabilized in normal ferroelectric phase. It is believed that c/a is a more appropriate parameter to illustrate the variation of ferroelectric properties for unfilled TTB system.

  4. Activation of particulate guanylate cyclase by adrenomedullin in cultured SV-40 transformed cat iris sphincter smooth muscle (SV-CISM-2) cells.

    PubMed

    Ali, N; Yousufzai, S Y; Abdel-Latif, A A

    2000-07-01

    We investigated the effects of adrenomedullin (ADM) on cGMP production in cultured SV-40 transformed cat iris sphincter smooth muscle (SV-CISM-2) cells. ADM increased cGMP accumulation in a time- and concentration- dependent manner. The peptide increased cGMP formation in the transformed cells by 405-fold as compared to 1. 6-fold in primary cultured CISM cells. The basal cGMP concentrations in both cell types were comparable. In addition, ADM increased cAMP accumulation in SV-CISM-2 cells and in primary cultured cells by 18. 9- and 5.8-fold, respectively. The ADM receptor antagonist, ADM(26-52), but not the atrial natriuretic peptide (ANP) receptor antagonist, anantin, inhibited ADM-induced cGMP formation. The phorbol ester, phorbol 12, 13-dibutyrate (PDBu), which inhibits particulate guanylate cyclases in smooth muscle, blocked ADM-stimulated cGMP accumulation. In contrast, inhibitors of the soluble guanylate cyclases, such as LY83583 and ODQ, and inhibitors of the nitric oxide cascade had little effect on ADM-stimulated cGMP production. The stimulatory effect of ADM on cGMP formation is due to activation of the guanylate cyclase system and not to a much reduced phosphodiesterase activity. ADM stimulated guanylate cyclase activity in membrane fractions isolated from SV-CISM-2 cells in a concentration-dependent manner with EC(50) value of 72 nM. Pertussis toxin, an activator of the G-protein, Gi, inhibited ADM-stimulated cGMP accumulation, whereas cholera toxin, a stimulator of the Gs G-protein and subsequently cAMP accumulation, had little effect. Pretreatment of the plasma membrane fraction with Gialpha antibody attenuated ADM-stimulated guanylate cyclase activity by 75%. We conclude that ADM increases intracellular cGMP levels in SV-CISM-2 cells through activation of the ADM receptor and subsequent stimulation of a Gi-mediated membrane-bound guanylate cyclase.

  5. 23 CFR 192.5 - Certification requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Certification requirements. 192.5 Section 192.5 Highways... Transportation by April 1, 1993 and by January 1 of each subsequent year that it meets the requirements of 23 U.S.C. 159 and this regulation. (b) If the State believes it meets the requirements of 23 U.S.C. 159 and...

  6. 23 CFR 192.5 - Certification requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Certification requirements. 192.5 Section 192.5 Highways... Transportation by April 1, 1993 and by January 1 of each subsequent year that it meets the requirements of 23 U.S.C. 159 and this regulation. (b) If the State believes it meets the requirements of 23 U.S.C. 159 and...

  7. 23 CFR 192.5 - Certification requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Certification requirements. 192.5 Section 192.5 Highways... Transportation by April 1, 1993 and by January 1 of each subsequent year that it meets the requirements of 23 U.S.C. 159 and this regulation. (b) If the State believes it meets the requirements of 23 U.S.C. 159 and...

  8. 23 CFR 192.5 - Certification requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Certification requirements. 192.5 Section 192.5 Highways... Transportation by April 1, 1993 and by January 1 of each subsequent year that it meets the requirements of 23 U.S.C. 159 and this regulation. (b) If the State believes it meets the requirements of 23 U.S.C. 159 and...

  9. 23 CFR 924.7 - Program structure.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Program structure. 924.7 Section 924.7 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HIGHWAY SAFETY HIGHWAY SAFETY IMPROVEMENT PROGRAM... implementation through highway safety improvement projects. The HSIP includes construction and operational...

  10. 23 CFR 924.7 - Program structure.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Program structure. 924.7 Section 924.7 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HIGHWAY SAFETY HIGHWAY SAFETY IMPROVEMENT PROGRAM... implementation through highway safety improvement projects. The HSIP includes construction and operational...

  11. Navy CG(X) Cruiser Program: Background for Congress

    DTIC Science & Technology

    2010-06-10

    PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES...Navy’s Top Officer Sees Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September 24, 2008) quoted Admiral Gary Roughead, the Chief...procuring CG(X)s was properly aligned with foreign-country ballistic missile development programs. A 2005 defense trade press report, for example, stated

  12. 23 CFR 637.207 - Quality assurance program.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Quality assurance program. 637.207 Section 637.207... CONSTRUCTION INSPECTION AND APPROVAL Quality Assurance Procedures for Construction § 637.207 Quality assurance program. (a) Each STD's quality assurance program shall provide for an acceptance program and an...

  13. 23 CFR 637.207 - Quality assurance program.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... independent assurance (IA) program consisting of the following: (1) Acceptance program. (i) Each STD's... quality control samples. (C) The quality control sampling and testing is evaluated by an IA program. (iii... appropriate. See 23 CFR 635.413(e) for specific requirements. (2) The IA program shall evaluate the qualified...

  14. Use of the VIP-Man model to calculate energy imparted and effective dose for x-ray examinations.

    PubMed

    Winslow, Mark; Huda, Walter; Xu, X George; Chao, T C; Shi, C Y; Ogden, Kent M; Scalzetti, Ernest M

    2004-02-01

    A male human tomographic model was used to calculate values of energy imparted (epsilon) and effective dose (E) for monoenergetic photons (30-150 keV) in radiographic examinations. Energy deposition in the organs and tissues of the human phantom were obtained using Monte Carlo simulations. Values of E/epsilon were obtained for three common projections [anterior-posterior (AP), posterior-anterior (PA), and lateral (LAT)] of the head, cervical spine, chest, and abdomen, respectively. For head radiographs, all three projections yielded similar E/epsilon values. At 30 keV, the value of E/epsilon was approximately 1.6 mSv J(-1), which is increased to approximately 7 mSv J(-1) for 150 keV photons. The AP cervical spine was the only projection investigated where the value of E/epsilon decreased with increasing photon energy. Above 70 keV, cervical spine E/epsilon values showed little energy dependence and ranged between approximately 8.5 mSv J(-1) for PA projections and approximately 17 mSv J(-1) for AP projections. The values of E/epsilon for AP chest examinations showed very little variation with photon energy, and had values of approximately 23 mSv J(-1). Values of E/epsilon for PA and LAT chest projections were substantially lower than the AP projections and increased with increasing photon energy. For abdominal radiographs, differences between the PA and LAT projections were very small. All abdomen projections showed an increase in the E/epsilon ratio with increasing photon energy, and reached a maximum value of approximately 13.5 mSv J(-1) for AP projections, and approximately 9.5 mSv J(-1) for PA/lateral projections. These monoenergetic E/epsilon values can generate values of E/epsilon for any x-ray spectrum, and can be used to convert values of energy imparted into effective dose for patients undergoing common head and body radiological examinations.

  15. 49 CFR 5.23 - Contents of notices.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false Contents of notices. 5.23 Section 5.23 Transportation Office of the Secretary of Transportation RULEMAKING PROCEDURES Procedures § 5.23 Contents of notices. (a) Each notice of proposed rulemaking is published in the Federal Register, unless all persons subject to it are named and are personally...

  16. 23 CFR 1.35 - Bonus program.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Bonus program. 1.35 Section 1.35 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL MANAGEMENT AND ADMINISTRATION GENERAL § 1.35 Bonus program. (a) Any agreement entered into by a State pursuant to the provisions of section 12 of the...

  17. 23 CFR 1.35 - Bonus program.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Bonus program. 1.35 Section 1.35 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL MANAGEMENT AND ADMINISTRATION GENERAL § 1.35 Bonus program. (a) Any agreement entered into by a State pursuant to the provisions of section 12 of the...

  18. 23 CFR 1.35 - Bonus program.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Bonus program. 1.35 Section 1.35 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL MANAGEMENT AND ADMINISTRATION GENERAL § 1.35 Bonus program. (a) Any agreement entered into by a State pursuant to the provisions of section 12 of the...

  19. 23 CFR 1.35 - Bonus program.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Bonus program. 1.35 Section 1.35 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL MANAGEMENT AND ADMINISTRATION GENERAL § 1.35 Bonus program. (a) Any agreement entered into by a State pursuant to the provisions of section 12 of the...

  20. Studies of Nano-structured Se77Sb23- x Ge x Thin Films Prepared by Physical Vapor Condensation Technique

    NASA Astrophysics Data System (ADS)

    Alvi, M. A.

    2017-02-01

    Bulk Se77Sb23- x Ge x material with x = 4 and 12 was prepared by employing a melt quench technique. Its amorphous as well as glassy nature was confirmed by x-ray diffraction analysis and nonisothermal differential scanning calorimetry measurements. The physical vapor condensation technique was applied to prepare nanostructured thin films of Se77Sb23- x Ge x material. The surface morphology of the films was examined using field-emission scanning electron microscopy, revealing average particle size between 20 nm and 50 nm. Systematic investigation of optical absorption data indicated that the optical transition was indirect in nature. The dark conductivity (dc conductivity) of nano-structured Se77Sb23- x Ge x thin films was also investigated at temperatures from 313 K to 463 K, revealing that it tended to increase with increasing temperature. Analyses of our experimental data also indicate that the conduction is due to thermally supported tunneling of charge carriers in confined states close to the band edges. The calculated values of activation energy agree well with the optical bandgap.

  1. 23 CFR 661.23 - How will a bridge project be programmed for funding once eligibility has been determined?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false How will a bridge project be programmed for funding once eligibility has been determined? 661.23 Section 661.23 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.23 How will a...

  2. 23 CFR 661.23 - How will a bridge project be programmed for funding once eligibility has been determined?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false How will a bridge project be programmed for funding once eligibility has been determined? 661.23 Section 661.23 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.23 How will a...

  3. 23 CFR 661.23 - How will a bridge project be programmed for funding once eligibility has been determined?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false How will a bridge project be programmed for funding once eligibility has been determined? 661.23 Section 661.23 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.23 How will a...

  4. 46 CFR 76.23-5 - Zoning.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS FIRE PROTECTION EQUIPMENT Manual Sprinkling System, Details § 76.23-5 Zoning. (a) Separate zones may be used for each deck, and on any particular... that the end sprinkler heads of both adjoining zones will cover the identical area. Table 76.23-5(b...

  5. 46 CFR 76.23-5 - Zoning.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS FIRE PROTECTION EQUIPMENT Manual Sprinkling System, Details § 76.23-5 Zoning. (a) Separate zones may be used for each deck, and on any particular... that the end sprinkler heads of both adjoining zones will cover the identical area. Table 76.23-5(b...

  6. 46 CFR 76.23-5 - Zoning.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS FIRE PROTECTION EQUIPMENT Manual Sprinkling System, Details § 76.23-5 Zoning. (a) Separate zones may be used for each deck, and on any particular... that the end sprinkler heads of both adjoining zones will cover the identical area. Table 76.23-5(b...

  7. 46 CFR 76.23-5 - Zoning.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS FIRE PROTECTION EQUIPMENT Manual Sprinkling System, Details § 76.23-5 Zoning. (a) Separate zones may be used for each deck, and on any particular... that the end sprinkler heads of both adjoining zones will cover the identical area. Table 76.23-5(b...

  8. 46 CFR 76.23-5 - Zoning.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS FIRE PROTECTION EQUIPMENT Manual Sprinkling System, Details § 76.23-5 Zoning. (a) Separate zones may be used for each deck, and on any particular... that the end sprinkler heads of both adjoining zones will cover the identical area. Table 76.23-5(b...

  9. 5 CFR 1205.23 - Time limits.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Time limits. 1205.23 Section 1205.23 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES PRIVACY ACT REGULATIONS Amendment of Records § 1205.23 Time limits. The Clerk of the Board, Regional Director, or Chief...

  10. 5 CFR 1205.23 - Time limits.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Time limits. 1205.23 Section 1205.23 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES PRIVACY ACT REGULATIONS Amendment of Records § 1205.23 Time limits. The Clerk of the Board, Regional Director, or Chief...

  11. 5 CFR 1205.23 - Time limits.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Time limits. 1205.23 Section 1205.23 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES PRIVACY ACT REGULATIONS Amendment of Records § 1205.23 Time limits. The Clerk of the Board, Regional Director, or Chief...

  12. 5 CFR 1205.23 - Time limits.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Time limits. 1205.23 Section 1205.23 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES PRIVACY ACT REGULATIONS Amendment of Records § 1205.23 Time limits. The Clerk of the Board, Regional Director, or Chief...

  13. 5 CFR 1205.23 - Time limits.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Time limits. 1205.23 Section 1205.23 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES PRIVACY ACT REGULATIONS Amendment of Records § 1205.23 Time limits. The Clerk of the Board, Regional Director, or Chief...

  14. 24 CFR 590.23 - Program close-out.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 3 2011-04-01 2010-04-01 true Program close-out. 590.23 Section 590.23 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN...

  15. 24 CFR 590.23 - Program close-out.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Program close-out. 590.23 Section 590.23 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN...

  16. 5 CFR 1215.23 - Other remedies.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Other remedies. 1215.23 Section 1215.23 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES DEBT MANAGEMENT Claims Collection § 1215.23 Other remedies. The remedies and sanctions available to the MSPB under this part are not...

  17. 5 CFR 1215.23 - Other remedies.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Other remedies. 1215.23 Section 1215.23 Administrative Personnel MERIT SYSTEMS PROTECTION BOARD ORGANIZATION AND PROCEDURES DEBT MANAGEMENT Claims Collection § 1215.23 Other remedies. The remedies and sanctions available to the MSPB under this part are not...

  18. 5 CFR 1639.23 - Hearing.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Hearing. 1639.23 Section 1639.23... Hearing. (a) Request for hearing. Except as provided in paragraph (b) of this section, an employee who desires a hearing concerning the existence or amount of the debt or the proposed offset schedule must send...

  19. 5 CFR 1639.23 - Hearing.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Hearing. 1639.23 Section 1639.23... Hearing. (a) Request for hearing. Except as provided in paragraph (b) of this section, an employee who desires a hearing concerning the existence or amount of the debt or the proposed offset schedule must send...

  20. 5 CFR 1639.23 - Hearing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Hearing. 1639.23 Section 1639.23... Hearing. (a) Request for hearing. Except as provided in paragraph (b) of this section, an employee who desires a hearing concerning the existence or amount of the debt or the proposed offset schedule must send...

  1. 5 CFR 1639.23 - Hearing.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Hearing. 1639.23 Section 1639.23... Hearing. (a) Request for hearing. Except as provided in paragraph (b) of this section, an employee who desires a hearing concerning the existence or amount of the debt or the proposed offset schedule must send...

  2. 5 CFR 1639.23 - Hearing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Hearing. 1639.23 Section 1639.23... Hearing. (a) Request for hearing. Except as provided in paragraph (b) of this section, an employee who desires a hearing concerning the existence or amount of the debt or the proposed offset schedule must send...

  3. P2X3 and P2X2/3 Receptors Play a Crucial Role in Articular Hyperalgesia Development Through Inflammatory Mechanisms in the Knee Joint Experimental Synovitis.

    PubMed

    Teixeira, Juliana Maia; Bobinski, Franciane; Parada, Carlos Amílcar; Sluka, Kathleen A; Tambeli, Cláudia Herrera

    2017-10-01

    Osteoarthritis (OA) is a degenerative and progressive disease characterized by cartilage breakdown and by synovial membrane inflammation, which results in disability, joint swelling, and pain. The purinergic P2X3 and P2X2/3 receptors contribute to development of inflammatory hyperalgesia, participate in arthritis processes in the knee joint, and are expressed in chondrocytes and nociceptive afferent fibers innervating the knee joint. In this study, we hypothesized that P2X3 and P2X2/3 receptors activation by endogenous ATP (adenosine 5'-triphosphate) induces articular hyperalgesia in the knee joint of male and female rats through an indirect sensitization of primary afferent nociceptors dependent on the previous release of pro-inflammatory cytokines and/or on neutrophil migration. We found that the blockade of articular P2X3 and P2X2/3 receptors significantly attenuated carrageenan-induced hyperalgesia in the knee joint of male and estrus female rats in a similar manner. The carrageenan-induced knee joint inflammation increased the expression of P2X3 receptors in chondrocytes of articular cartilage. Further, the blockade of articular P2X3 and P2X2/3 receptors significantly reduced the increased concentration of TNF-α, IL-6, and CINC-1 and the neutrophil migration induced by carrageenan. These findings indicate that P2X3 and P2X2/3 receptors activation by endogenous ATP is essential to hyperalgesia development in the knee joint through an indirect sensitization of primary afferent nociceptors dependent on the previous release of pro-inflammatory cytokines and/or on neutrophil migration.

  4. Design, synthesis, biological evaluation and X-ray crystal structure of novel classical 6,5,6-tricyclic benzo[4,5]thieno[2,3-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors

    PubMed Central

    Zhang, Xin; Zhou, Xilin; L.Kisliuk, Roy; Piraino, Jennifer; Cody, Vivian

    2011-01-01

    Classical antifolates (4-7) with a tricyclic benzo[4,5]thieno[2,3-d]pyrimidine scaffold and a flexible and rigid benzoylglutamate were synthesized as dual thymidylate synthase (TS) and dihydrofolate reductase (DHFR) inhibitors. Oxidative aromatization of ethyl 2-amino-4-methyl-4,5,6,7-tetrahydro-1-benzothiophene-3-carboxylate (±)-9 to ethyl 2-amino-4-methyl-1-benzothiophene-3-carboxylate 10 with 10% Pd/C was a key synthetic step. Compounds with 2-CH3 substituents inhibited human (h) TS (IC50 = 0.26-0.8 μM), but not hDHFR. Substitution of the 2-CH3 with a 2-NH2 increases hTS inhibition by more than 10-fold and also affords excellent hDHFR inhibition (IC50 = 0.09-0.1 μM). This study shows that the tricyclic benzo[4,5]thieno[2,3-d]pyrimidine scaffold is highly conducive to single hTS or dual hTS-hDHFR inhibition depending on the 2-position substituents. The X-ray crystal structures of 6 and 7 with hDHFR reveal, for the first time, that tricyclics 6 and 7 bind with the benzo[4,5]thieno[2,3-d]pyrimidine ring in the folate binding mode with the thieno S mimicking the 4-amino of methotrexate. PMID:21550809

  5. Navy CG(X) Cruiser Program: Background for Congress

    DTIC Science & Technology

    2010-04-08

    PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES...Inside the Navy, October 27, 2008. Another press report (Katherine McIntire Peters, “Navy’s Top Officer Sees Lessons in Shipbuilding Program Failures...have changed.37 A related question was whether the schedule for procuring CG(X)s was properly aligned with foreign-country ballistic missile

  6. Why are SiX5(-) and GeX5(-) (X = F, Cl) stable but not CF5(-) and CCl5(-)?

    PubMed

    Marchaj, Marzena; Freza, Sylwia; Skurski, Piotr

    2012-03-01

    The possible existence of the CF(5)(-), CCl(5)(-), SiF(5)(-), SiCl(5)(-), GeF(5)(-), and GeCl(5)(-) anions has been investigated using ab initio methods. The species containing Si and Ge as central atoms were found to adopt the D(3h)-symmetry trigonal bipyramidal equilibrium structures whose thermodynamic stabilities were confirmed by examining the most probable fragmentation channels. The ab initio re-examination of the electronic stabilities of the SiF(5)(-), SiCl(5)(-), GeF(5)(-), and GeCl(5)(-) anions [using the OVGF(full) method with the 6-311+G(3df) basis set] led to the very large vertical electron detachment (VDE) energies of 9.316 eV (SiF(5)(-)) and 9.742 eV (GeF(5)(-)), whereas smaller VDEs of 6.196 and 6.452 eV were predicted for the SiCl(5)(-) and GeCl(5)(-) species, respectively. By contrast, the high-symmetry and structurally compact anionic CF(5)(-) and CCl(5)(-) systems cannot exist due to the strongly repulsive potential predicted for the X(-) (F(-) or Cl(-)) approaching the CX(4) (CF(4) or CCl(4)). The formation of weakly bound CX(4)···X(-) (CF(4)···F(-) and CCl(4)···Cl(-)) anionic complexes (consisting of pseudotetrahedral neutral CX(4) with the weakly tethered X(-)) might be expected at low temperatures (approaching 0 K), whereas neither CX(5)(-) (CF(5)(-), CCl(5)(-)) systems nor CX(4)···X(-) (CF(4)···F(-) and CCl(4)···Cl(-)) complexes can exist in the elevated temperatures (above 0K) due to their susceptibility to the fragmentation (leading to the X(-) loss). © 2012 American Chemical Society

  7. Line-profile and continuum variations of the contact binary SV Centauri

    NASA Technical Reports Server (NTRS)

    Rahe, J.; Drechsel, H.; Wargau, W.

    1982-01-01

    A total of five high and ten low dispersion UV spectra of the interacting contact binary SV Centauri obtained between 1979 and 1982 are analyzed. The low resolution observations cover the whole phase range, while a few selected phases were observed in high dispersion. The UV data were complemented with optical photometric and spectroscopic observations, in order to determine the tructure and absolute dimensions of the system. The profiles of prominent UV resonance and metastable lines undergo drastic changes with phase angle and time. Their overall appearance indicates relatively strong mass loss from the system, exhibiting pronounced variations of the stellar wind. The far UV continuum distribution suggests the presence of a luminous hot radiation source with maximum emission in the soft X-ray range, which is most apparently seen during the first quadrature phase, while it is weakest close to primary minimum. The case exchange and mass loss process as well as the evolutionary stage of SV Centauri are discussed.

  8. 5 CFR 1620.23 - Spousal rights.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Spousal rights. 1620.23 Section 1620.23... and Judges § 1620.23 Spousal rights. (a) The current spouse of a justice or judge of the United States (as defined in 28 U.S.C. 451), or of a Court of Veterans Appeals judge, possesses the rights described...

  9. Navy CG(X) Cruiser Program: Background, Oversight Issues, and Options for Congress

    DTIC Science & Technology

    2009-06-04

    Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK UNIT...Inside the Navy, October 27, 2008. Another press report (Katherine McIntire Peters, “Navy’s Top Officer Sees Lessons in Shipbuilding Program...X)s could change.38 A related question is whether the schedule for procuring CG(X)s is properly aligned with foreign-country ballistic missile

  10. Hyper-X Program Status

    NASA Technical Reports Server (NTRS)

    McClinton, Charles R.; Rausch, Vincent L.; Sitz, Joel; Reukauf, Paul

    2001-01-01

    This paper provides an overview of the objectives and status of the Hyper-X program, which is tailored to move hypersonic, airbreathing vehicle technology from the laboratory environment to the flight environment. The first Hyper-X research vehicle (HXRV), designated X-43, is being prepared at the Dryden Flight Research Center for flight at Mach 7. Extensive risk reduction activities for the first flight are completed, and non-recurring design activities for the Mach 10 X-43 (3rd flight) are nearing completion. The Mach 7 flight of the X-43, in the spring of 2001, will be the first flight of an airframe-integrated scramjet-powered vehicle. The Hyper-X program is continuing to plan follow-on activities to focus an orderly continuation of hypersonic technology development through flight research.

  11. Hyper-X Program Status

    NASA Technical Reports Server (NTRS)

    McClinton, Charles R.; Reubush, David E.; Sitz, Joel; Reukauf, Paul

    2001-01-01

    This paper provides an overview of the objectives and status of the Hyper-X program, which is tailored to move hypersonic, airbreathing vehicle technology from the laboratory environment to the flight environment. The first Hyper-X research vehicle (HXRV), designated X-43, is being prepared at the Dryden Flight Research Center for flight at Mach 7. Extensive risk reduction activities for the first flight are completed, and non-recurring design activities for the Mach 10 X-43 (third flight) are nearing completion. The Mach 7 flight of the X-43, in the spring of 2001, will be the first flight of an airframe-integrated scramjet-powered vehicle. The Hyper-X program is continuing to plan follow-on activities to focus an orderly continuation of hypersonic technology development through flight research.

  12. 23 CFR 669.5 - Objective.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Objective. 669.5 Section 669.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS ENFORCEMENT OF HEAVY VEHICLE USE TAX § 669.5 Objective. The objective of this regulation is to establish realistic and workable...

  13. Approaching Piezoelectric Response of Pb-Piezoelectrics in Hydrothermally Synthesized Bi0.5(Na1- xK x)0.5TiO3 Nanotubes.

    PubMed

    Ghasemian, Mohammad Bagher; Rawal, Aditya; Liu, Yun; Wang, Danyang

    2018-06-20

    A large piezoelectric coefficient of 76 pm/V along the diameter direction, approaching that of lead-based piezoelectrics, is observed in hydrothermally synthesized Pb-free Bi 0.5 (Na 0.8 K 0.2 ) 0.5 TiO 3 nanotubes. The 30-50 nm diameter nanotubes are formed through a scrolling and wrapping mechanism without the need of a surfactant or template. A molar ratio of KOH/NaOH = 0.5 for the mineralizers yields the Na/K ratio of ∼0.8:0.2, corresponding to an orthorhombic-tetragonal (O-T) phase boundary composition. X-ray diffraction patterns along with transmission electron microscopy analysis ascertain the coexistence of orthorhombic and tetragonal phases with (110) and (001) orientations along the nanotube length direction, respectively. 23 Na NMR spectroscopy confirms the higher degree of disorder in Bi 0.5 (Na 1- x K x ) 0.5 TiO 3 nanotubes with O-T phase coexistence. These findings present a significant advance toward the application of Pb-free piezoelectric materials.

  14. Navy CG(X) Cruiser Program: Background, Oversight Issues, and Options for Congress

    DTIC Science & Technology

    2009-07-17

    Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK...McIntire Peters, “Navy’s Top Officer Sees Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September 24, 2008) quoted Admiral Gary...defined, the total required number of CG(X)s could change.38 A related question is whether the schedule for procuring CG(X)s is properly aligned

  15. 23 CFR 751.25 - Programming and authorization.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Programming and authorization. 751.25 Section 751.25 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT JUNKYARD CONTROL AND ACQUISITION § 751.25 Programming and authorization. (a) Junkyard control projects shall be...

  16. 23 CFR 751.25 - Programming and authorization.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Programming and authorization. 751.25 Section 751.25 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT JUNKYARD CONTROL AND ACQUISITION § 751.25 Programming and authorization. (a) Junkyard control projects shall be...

  17. 23 CFR 751.25 - Programming and authorization.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Programming and authorization. 751.25 Section 751.25 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT JUNKYARD CONTROL AND ACQUISITION § 751.25 Programming and authorization. (a) Junkyard control projects shall be...

  18. 23 CFR 751.25 - Programming and authorization.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Programming and authorization. 751.25 Section 751.25 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT JUNKYARD CONTROL AND ACQUISITION § 751.25 Programming and authorization. (a) Junkyard control projects shall be...

  19. 23 CFR 751.25 - Programming and authorization.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Programming and authorization. 751.25 Section 751.25 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT JUNKYARD CONTROL AND ACQUISITION § 751.25 Programming and authorization. (a) Junkyard control projects shall be...

  20. 23 CFR 668.215 - Programming and project procedures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

  1. 23 CFR 668.215 - Programming and project procedures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

  2. 23 CFR 668.215 - Programming and project procedures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

  3. 23 CFR 668.215 - Programming and project procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

  4. 23 CFR 668.215 - Programming and project procedures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Programming and project procedures. 668.215 Section 668.215 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal Agencies for Federal Roads § 668.215 Programming...

  5. 38 CFR 23.400 - Education programs or activities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 23...

  6. 23 CFR 668.113 - Program and project procedures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Program and project procedures. 668.113 Section 668.113 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS EMERGENCY RELIEF PROGRAM Procedures for Federal-Aid Highways § 668.113 Program and project procedures. (a...

  7. 38 CFR 23.400 - Education programs or activities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Education programs or... (CONTINUED) NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 23...

  8. 23 CFR 661.23 - How will a bridge project be programmed for funding once eligibility has been determined?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false How will a bridge project be programmed for funding once... TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.23 How will a bridge project be programmed for funding once eligibility has been determined? (a) All projects will be...

  9. 23 CFR 661.23 - How will a bridge project be programmed for funding once eligibility has been determined?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false How will a bridge project be programmed for funding once... TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS INDIAN RESERVATION ROAD BRIDGE PROGRAM § 661.23 How will a bridge project be programmed for funding once eligibility has been determined? (a) All projects will be...

  10. Eu2+-Doped Sr2B2-2xSi2+3xAl2-xN8+x: A Boron-Containing Orange-Emitting Nitridosilicate with Interesting Composition-Dependent Photoluminescence Properties.

    PubMed

    Ten Kate, Otmar M; Xie, Rong-Jun; Wang, Chun-Yun; Funahashi, Shiro; Hirosaki, Naoto

    2016-11-07

    Novel Sr 2-y Eu y B 2-2x Si 2+3x Al 2-x N 8+x phosphors were investigated as a function of the boron and aluminum over silicon ratio and as a function of the Eu 2+ concentration. Samples were prepared via solid-state reaction synthesis by carefully controlling the synthesis conditions and composition. At high boron and aluminum content, that is, x = 0, a Eu 2+ 5d-4f emission is observed of which the maximum shifts from 595 nm for low Eu concentrations (y = 0.005) toward 623 nm for high Eu concentrations (y = 0.5). The samples can be excited by UV or blue light up to ∼475 nm. Substitution of [B 2 Al] 9+ units by [Si 3 N] 9+ units, increasing x up to 0.15, greatly improves the luminescence efficiency up to 46% and shows a very large redshift of the excitation bands with ∼100 nm, while the emission band shifts with ∼10 nm. The shifts are attributed to the lowering of the 5d level as a result of the decreased Eu-N distance upon substitution. Temperature-dependent measurements show that the Eu 2+ 5d-4f emission is largely thermally quenched at room temperature for x = 0 due to thermal ionization toward the conduction band, explaining the low luminescence efficiency. The lowering of the 5d level at larger values of x reduces the thermal ionization and consequently increases the thermal stability and quantum efficiency, resulting in strongly luminescent blue-to-orange conversion phosphors that are interesting for light-emitting diode applications.

  11. 34 CFR 300.23 - Individualized education program team.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Individualized education program team. 300.23 Section 300.23 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE...

  12. 5 CFR 2423.23 - Prehearing disclosure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Prehearing disclosure. 2423.23 Section 2423.23 Administrative Personnel FEDERAL LABOR RELATIONS AUTHORITY, GENERAL COUNSEL OF THE FEDERAL..., with an index, proposed to be offered into evidence; and (c) Theories. A brief statement of the theory...

  13. Performance of mobile digital X-ray fluoroscopy using a novel flat panel detector for intraoperative use.

    PubMed

    Jeong, Chang-Won; Ryu, Jong-Hyun; Joo, Su-Chong; Jun, Hong-Young; Heo, Dong-Woon; Lee, Jinseok; Kim, Kyong-Woo; Yoon, Kwon-Ha

    2015-01-01

    Technologies employing digital X-ray devices are developed for mobile settings. To develop a mobile digital X-ray fluoroscopy (MDF) for intraoperative guidance, using a novel flat panel detector to focus on diagnostics in outpatient clinics, operating and emergency rooms. An MDF for small-scale field diagnostics was configured using an X-ray source and a novel flat panel detector. The imager enabled frame rates reaching 30 fps in full resolution fluoroscopy with maximal running time of 5 minutes. Signal-to-noise (SNR), contrast-to-noise (CNR), and spatial resolution were analyzed. Stray radiation, exposure radiation dose, and effective absorption dose were measured for patients. The system was suitable for small-scale field diagnostics. SNR and CNR were 62.4 and 72.0. Performance at 10% of MTF was 9.6 lp/mm (53 μ m) in the no binned mode. Stray radiation at 100 cm and 150 cm from the source was below 0.2 μ Gy and 0.1 μ Gy. Exposure radiation in radiography and fluoroscopy (5 min) was 10.2 μ Gy and 82.6 mGy. The effective doses during 5-min-long fluoroscopy were 0.26 mSv (wrist), 0.28 mSv (elbow), 0.29 mSv (ankle), and 0.31 mSv (knee). The proposed MDF is suitable for imaging in operating rooms.

  14. 23 CFR 172.5 - Methods of procurement.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Methods of procurement. 172.5 Section 172.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PAYMENT PROCEDURES ADMINISTRATION OF... to 23 U.S.C. 112(b)(2) in accordance with 49 CFR part 26. (c) Compensation. The cost plus a...

  15. 23 CFR 172.5 - Methods of procurement.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Methods of procurement. 172.5 Section 172.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PAYMENT PROCEDURES ADMINISTRATION OF... to 23 U.S.C. 112(b)(2) in accordance with 49 CFR part 26. (c) Compensation. The cost plus a...

  16. A New Observation of the Quiet Sun Soft X-ray (0.5-5 keV) Spectrum

    NASA Astrophysics Data System (ADS)

    Caspi, A.; Woods, T. N.; Stone, J.

    2012-12-01

    The solar corona is the brightest source of X-rays in the solar system, and the X-ray emission is highly variable with solar activity. While this is particularly true during solar flares, when emission can be enhanced by many orders of magnitude up to gamma-ray energies, even the so-called "quiet Sun" is bright in soft X-rays (SXRs), as the ~1-2 MK ambient plasma of the corona emits significant thermal bremsstrahlung up to ~5 keV. However, the actual solar SXR (0.5-5 keV) spectrum is not well known, particularly during quiet periods, as, with few exceptions, this energy range has not been systematically studied in many years. Previous observations include ultra-high-resolution but very narrow-band spectra from crystral spectrometers (e.g. Yohkoh/BCS), or integrated broadband irradiances from photometers (e.g. GOES/XRS, TIMED/XPS, etc.) that lack detailed spectral information. In recent years, broadband measurements with fair energy resolution (~0.5-0.7 keV FWHM) were made by SphinX on CORONAS-Photon and XRS on MESSENGER, although they did not extend below ~1 keV. We present observations of the quiet Sun SXR emission obtained using a new SXR spectrometer flown on the third SDO/EVE underflight calibration rocket (NASA 36.286). The commercial off-the-shelf Amptek X123 silicon drift detector, with an 8-micron Be window and custom aperture, measured the solar SXR emission from ~0.5 to >10 keV with ~0.15 keV FWHM resolution (though, due to hardware limitations, with only ~0.12 keV binning) and 2-sec cadence over ~5 minutes on 23 June 2012. Despite the rising solar cycle, activity on 23 June 2012 was abnormally low, with no visible active regions and GOES XRS emission near 2010 levels; we measured no solar counts above ~4 keV during the observation period. We compare our X123 measurements with spectra and broadband irradiances from other instruments, including the SphinX observations during the deep solar minimum of 2009, and with upper limits of >3 keV quiet Sun emission

  17. A New Observation of the Quiet Sun Soft X-ray (0.5-5 keV) Spectrum

    NASA Astrophysics Data System (ADS)

    Caspi, Amir; Woods, Thomas N.; Stone, Jordan

    2013-03-01

    The solar corona is the brightest source of X-rays in the solar system, and the X-ray emission is highly variable with solar activity. While this is particularly true during solar flares, when emission can be enhanced by many orders of magnitude up to gamma-ray energies, even the so-called "quiet Sun" is bright in soft X-rays (SXRs), as the 1-2 MK ambient plasma of the corona emits significant thermal bremsstrahlung up to 5 keV. However, the actual solar SXR (0.5-5 keV) spectrum is not well known, particularly during quiet periods, as, with few exceptions, this energy range has not been systematically studied in many years. Previous observations include ultra-high-resolution but very narrow-band spectra from crystral spectrometers (e.g. Yohkoh/BCS), or integrated broadband irradiances from photometers (e.g. GOES/XRS, TIMED/XPS, etc.) that lack detailed spectral information. In recent years, broadband measurements with fair energy resolution ( 0.5-0.7 keV FWHM) were made by SphinX on CORONAS-Photon and XRS on MESSENGER, although they did not extend below 1 keV. We present observations of the quiet Sun SXR emission obtained using a new SXR spectrometer flown on the third SDO/EVE underflight calibration rocket (NASA 36.286). The commercial off-the-shelf Amptek X123 silicon drift detector, with an 8-micron Be window and custom aperture, measured the solar SXR emission from 0.5 to >10 keV with 0.15 keV FWHM resolution (though, due to hardware limitations, with only 0.12 keV binning) and 2-sec cadence over 5 minutes on 23 June 2012. Despite the rising solar cycle, activity on 23 June 2012 was abnormally low, with no visible active regions and GOES XRS emission near 2010 levels; we measured no solar counts above 4 keV during the observation period. We compare our X123 measurements with spectra and broadband irradiances from other instruments, including the SphinX observations during the deep solar minimum of 2009, and with upper limits of >3 keV quiet Sun emission

  18. Will X-ray Safety Glasses Become Mandatory for Radiological Vascular Interventions?

    PubMed

    Thomas, Rohit Philip; Grau, Mathias; Eldergash, Osama; Kowald, Tobias; Schnabel, Johannes; Szczechowicz, Marcin; Chavan, Ajay

    2018-07-01

    The annual permissible radiation ocular lens dose has been reduced to 20 millisieverts (mSv) in the current European directive 2013/59/Euratom. The aim of this study was to evaluate the personal radiation dose for vascular interventions with special focus on ocular lens dose. From May 2016 to October 2016, the personal radiation doses of two interventionists and four technicians were prospectively recorded during 206 vascular interventions. The position of personnel, intervention type and fluoroscopy time were recorded. Parameters evaluated were total body dose measured by film dosimeter, hand dose measured by ring thermoluminescent dosimeter (TLD) and ocular lens dose measured by TLD placed in front of the safety glasses. Linear regression analysis was used to estimate the dose at 2 and 5 years. The ocular lens dose, hand and total body dose of the two interventionists were 11/5, 56/47 and 0.6 mSv each, respectively. The estimated 5-year ocular dose was 113.08 mSv (95% CI 38.2-187.97)/40.95 (95% CI 16.9-64.7). Similarly, hand dose was 608.4 mSv (95% CI 442.78-774.38)/514.47 (95% CI 329.83-699.10) and body dose 6.07 mSv (95% CI 4.70-8.22)/5.12 (95% CI 3.65-6.59), respectively. Amongst four technicians, only the first assistant showed recordings of 0.3 mSv body dose, 2 mSv ocular lens dose and 5 mSv hand dose. The yearly ocular lens dose, particularly for interventionists dealing with complex interventions, could cross the permitted yearly limit set by the new Euratom directive. Therefore, X-ray safety glasses would become mandatory for complex radiological vascular interventions. Level III, non-randomized controlled cohort/follow-up study.

  19. Effects of Ga substitution in Ce:Tb3Ga x Al5- x O12 single crystals for scintillator applications

    NASA Astrophysics Data System (ADS)

    Nakauchi, Daisuke; Okada, Go; Kawano, Naoki; Kawaguchi, Noriaki; Yanagida, Takayuki

    2018-02-01

    Bulk single crystals of Ce-doped Tb3Ga x Al5- x O12 (x = 0-4) were successfully synthesized by the floating zone method. The samples exhibit photoluminescence and scintillation with an intense broad emission due to the 5d-4f transitions of Ce3+ peaking around 550 nm as well as a few sharp peaks due to the 4f-4f transitions of Tb3+. Pulse height spectrum measurements under 137Cs γ-ray irradiation demonstrated a clear photoabsorption peak, in which the scintillation light yields were estimated to be 57,000 (x = 0), 28,000 (x = 1), 19,000 (x = 2), and 10,000 (x = 3) photons/MeV. Afterglow level can be suppressed with an appropriate addition of Ga, in which the optimum concertation is x = 2 leading an afterglow level of 23 ppm.

  20. The Nature, Significance, and Evaluation of the Schwarzschild-Villiger (SV) Effect in Photometric Procedures

    PubMed Central

    Howling, D. H.; Fitzgerald, P. J.

    1959-01-01

    The Schwarzschild-Villiger effect has been experimentally demonstrated with the optical system used in this laboratory. Using a photographic mosaic specimen as a model, it has been shown that the conclusions of Naora are substantiated and that the SV effect, in large or small magnitude, is always present in optical systems. The theoretical transmission error arising from the presence of the SV effect has been derived for various optical conditions of measurement. The results have been experimentally confirmed. The SV contribution of the substage optics of microspectrophotometers has also been considered. A simple method of evaluating a flare function f(A) is advanced which provides a measure of the SV error present in a system. It is demonstrated that measurements of specimens of optical density less than unity can be made with less than 1 per cent error, when using illuminating beam diameter/specimen diameter ratios of unity and uncoated optical surfaces. For denser specimens it is shown that care must be taken to reduce the illuminating beam/specimen diameter ratio to a value dictated by the magnitude of a flare function f(A), evaluated for a particular optical system, in order to avoid excessive transmission error. It is emphasized that observed densities (transmissions) are not necessarily true densities (transmissions) because of the possibility of SV error. The ambiguity associated with an estimation of stray-light error by means of an opaque object has also been demonstrated. The errors illustrated are not necessarily restricted to microspectrophotometry but may possibly be found in such fields as spectral analysis, the interpretation of x-ray diffraction patterns, the determination of ionizing particle tracks and particle densities in photographic emulsions, and in many other types of photometric analysis. PMID:14403512

  1. Helical Poly(5-alkyl-2,3-thiophene)s: Controlled Synthesis and Structure Characterization

    DOE PAGES

    Zhang, Hong-Hai; Ma, Chuanxu; Bonnesen, Peter V.; ...

    2016-07-12

    Whereas Poly(3-alkyl-2,5-thiophene)s (P3AT), with many potential applications, have been extensively investigated, their ortho-connected isomers, poly(5-alkyl-2,3-thiophene)s (P5AT), have never been reported because of the difficulty in their syntheses. We herein present the first synthesis of regioregular P5AT via controlled Suzuki cross-coupling polymerization with PEPPSI-IPr as catalyst, affording the polymers with tunable molecular weight, narrow polydispersity (PDI) and well-defined functional end groups at the gram scale. The helical geometry of P5AT was studied by a combination of NMR, small angle x-ray scattering (SAXS) and scanning tunneling microscopy (STM). Particularly, the single polymer chain of poly(5- 2 butyl-2,3-thiophene) (P5BT) on highly oriented pyrolyticmore » graphite (HOPG) substrates with either M or P helical conformation was directly observed by STM. The comparison of UV-vis absorption between poly(5-hexyl-2,3-thiophene) (P5HT) (λ = 345 nm) and poly(3-hexyl-2,5- thiophene) (P3HT) (λ = 450 nm) indicated that the degree of conjugation of the backbone in P5HT is less than in P3HT, which may be a consequence of the helical geometry of the former compared to the more planar geometry of the latter. Moreover, we found that P5HT can emit green fluorescence under UV (λ = 360 nm) irradiation« less

  2. The NASA Hyper-X Program

    NASA Technical Reports Server (NTRS)

    Freeman, Delman C., Jr.; Reubush, Daivd E.; McClinton, Charles R.; Rausch, Vincent L.; Crawford, J. Larry

    1997-01-01

    This paper provides an overview of NASA's Hyper-X Program; a focused hypersonic technology effort designed to move hypersonic, airbreathing vehicle technology from the laboratory environment to the flight environment. This paper presents an overview of the flight test program, research objectives, approach, schedule and status. Substantial experimental database and concept validation have been completed. The program is currently concentrating on the first, Mach 7, vehicle development, verification and validation in preparation for wind-tunnel testing in 1998 and flight testing in 1999. Parallel to this effort the Mach 5 and 10 vehicle designs are being finalized. Detailed analytical and experimental evaluation of the Mach 7 vehicle at the flight conditions is nearing completion, and will provide a database for validation of design methods once flight test data are available.

  3. RAPTR-SV: a hybrid method for the detection of structural variants

    USDA-ARS?s Scientific Manuscript database

    Motivation: Identification of Structural Variants (SV) in sequence data results in a large number of false positive calls using existing software, which overburdens subsequent validation. Results: Simulations using RAPTR-SV and another software package that uses a similar algorithm for SV detection...

  4. Stimulation of NADH-dependent microsomal DNA strand cleavage by rifamycin SV.

    PubMed

    Kukiełka, E; Cederbaum, A I

    1995-04-15

    Rifamycin SV is an antibiotic anti-bacterial agent used in the treatment of tuberculosis. This drug can autoxidize, especially in the presence of metals, and generate reactive oxygen species. A previous study indicated that rifamycin SV can increase NADH-dependent microsomal production of reactive oxygen species. The current study evaluated the ability of rifamycin SV to interact with iron and increase microsomal production of hydroxyl radical, as detected by conversion of supercoiled plasmid DNA into the relaxed open circular state. The plasmid used was pBluescript II KS(-), and the forms of DNA were separated by agarose-gel electrophoresis. Incubation of rat liver microsomes with plasmid plus NADH plus ferric-ATP caused DNA strand cleavage. The addition of rifamycin SV produced a time- and concentration-dependent increase in DNA-strand cleavage. No stimulation by rifamycin SV occurred in the absence of microsomes, NADH or ferric-ATP. Stimulation occurred with other ferric complexes besides ferric-ATP, e.g. ferric-histidine, ferric-citrate, ferric-EDTA, and ferric-(NH4)2SO4. Rifamycin SV did not significantly increase the high rates of DNA strand cleavage found with NADPH as the microsomal reductant. The stimulation of NADH-dependent microsomal DNA strand cleavage was completely blocked by catalase, superoxide dismutase, GSH and a variety of hydroxyl-radical-scavenging agents, but not by anti-oxidants that prevent microsomal lipid peroxidation. Redox cycling agents, such as menadione and paraquat, in contrast with rifamycin SV, stimulated the NADPH-dependent reaction; menadione and rifamycin SV were superior to paraquat in stimulating the NADH-dependent reaction. These results indicate that rifamycin SV can, in the presence of an iron catalyst, increase microsomal production of reactive oxygen species which can cause DNA-strand cleavage. In contrast with other redox cycling agents, the stimulation by rifamycin SV is more pronounced with NADH than with NADPH as the

  5. (Ba1- x Bi0.33 x Sr0.67 x )(Ti1- x Bi0.67 x V0.33 x )O3 and (Ba1- x Bi0.5 x Sr0.5 x )(Ti1- x Bi0.5 x Ti0.5 x )O3 solid solutions: phase evolution, microstructure, dielectric properties and impedance analysis

    NASA Astrophysics Data System (ADS)

    Chen, Xiuli; Li, Xiaoxia; Yan, Xiao; Liu, Gaofeng; Zhou, Huanfu

    2018-06-01

    Perovskite solid solution ceramics of (Ba1- x Bi0.33 x Sr0.67 x )(Ti1- x Bi0.67 x V0.33 x )O3 and (Ba1- x Bi0.5 x Sr0.5 x )(Ti1- x Bi0.5 x Ti0.5 x )O3 (BBSTBV, BBSTBT, 0.02 ≤ x ≤ 0.2) were prepared by the traditional solid state reaction technique. The phase evolution, microstructure and dielectric properties of BBSTBV and BBSTBT ceramics were researched. X-Ray diffraction results illustrated that both BBSTBV and BBSTBT could form a homogenous solid solution which has a similar structure with BaTiO3. The optimized properties of (Ba0.8Bi0.1Sr0.1)(Ti0.8Bi0.1Ti0.1)O3 ceramics with stable ɛ r ( 1769-2293), small Δ ɛ/ ɛ 25 °C values (± 15%) over a broad temperature range from - 58 to 151 °C and low tan δ ≤ 0.03 from - 11 to 131 °C were obtained. In the high-temperature region, the relaxation and conduction process are attributed to the thermal activation and the oxygen vacancies may be the ionic charge carriers in perovskite ferroelectrics.

  6. Spontaneous firing and evoked responses of spinal nociceptive neurons are attenuated by blockade of P2X3 and P2X2/3 receptors in inflamed rats.

    PubMed

    Xu, Jun; Chu, Katharine L; Brederson, Jill-Desiree; Jarvis, Michael F; McGaraughty, Steve

    2012-08-01

    P2X3 and P2X2/3 receptors are selectively expressed on primary afferent nociceptors and have been implicated in modulating nociception in different models of pathological pain, including inflammatory pain. In an effort to delineate further the role of P2X3 receptors (homomeric and heteromeric) in the modulation of nociceptive transmission after a chronic inflammation injury, A-317491, a potent and selective P2X3-P2X2/3 antagonist, was administered to CFA-inflamed rats in order to examine its effects on responses of spinal dorsal horn neurons to mechanical and thermal stimulation. Systemic injection of A-317491 (30 μmol/kg, i.v.) reduced the responses of wide-dynamic-range (WDR) and nociceptive specific (NS) neurons to both high-intensity mechanical (pinch) and heat (49°C) stimulation. A-317491 also decreased low-intensity (10 g von Frey hair) mechanically evoked activity of WDR neurons but did not alter WDR neuronal responses to cold stimulation (5°C). Spontaneous firing of WDR neurons in CFA-inflamed rats was also significantly attenuated by A-317491 injection. By using immunohistochemistry, P2X3 receptors were demonstrated to be enhanced in lamina II of the spinal dorsal horn after inflammation. In summary, blockade of P2X3 and P2X2/3 receptors dampens mechanical- and heat-related signaling, as well as nonevoked activity of key classes of spinal nociceptive neurons in inflamed animals. These data suggest that P2X3 and/or P2X2/3 receptors have a broad contribution to somatosensory/nociceptive transmission in rats with a chronic inflammatory injury and are consistent with previous behavioral data demonstrating antiallodynic and antihyperalgesic effects of receptor antagonists. Copyright © 2012 Wiley Periodicals, Inc.

  7. 7 CFR 1412.23 - Base acres and Conservation Reserve Program.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false Base acres and Conservation Reserve Program. 1412.23... Base Acres for a Farm for Covered Commodities § 1412.23 Base acres and Conservation Reserve Program. (a... year, adjust the base acres for covered commodities and peanuts with respect to the farm by the number...

  8. 7 CFR 1412.23 - Base acres and Conservation Reserve Program.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false Base acres and Conservation Reserve Program. 1412.23... Base Acres for a Farm for Covered Commodities § 1412.23 Base acres and Conservation Reserve Program. (a... year, adjust the base acres for covered commodities and peanuts with respect to the farm by the number...

  9. 7 CFR 1412.23 - Base acres and Conservation Reserve Program.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Base acres and Conservation Reserve Program. 1412.23... Base Acres for a Farm for Covered Commodities § 1412.23 Base acres and Conservation Reserve Program. (a... year, adjust the base acres for covered commodities and peanuts with respect to the farm by the number...

  10. Navy CG(X) Cruiser Program: Background, Oversight Issues, and Options for Congress

    DTIC Science & Technology

    2009-04-10

    Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK...Sees Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September 24, 2008) quoted Admiral Gary Roughead, the Chief of Naval...question is whether the schedule for procuring CG(X)s is properly aligned with foreign-country ballistic missile development programs. A 2005 defense

  11. 23 CFR 511.311 - Real-time information program establishment.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... INFRASTRUCTURE MANAGEMENT REAL-TIME SYSTEM MANAGEMENT INFORMATION PROGRAM Real-Time System Management Information... operated by the State. In addition, the real-time information program shall complement current... 23 Highways 1 2011-04-01 2011-04-01 false Real-time information program establishment. 511.311...

  12. 23 CFR 511.311 - Real-time information program establishment.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... INFRASTRUCTURE MANAGEMENT REAL-TIME SYSTEM MANAGEMENT INFORMATION PROGRAM Real-Time System Management Information... operated by the State. In addition, the real-time information program shall complement current... 23 Highways 1 2014-04-01 2014-04-01 false Real-time information program establishment. 511.311...

  13. 23 CFR 511.311 - Real-time information program establishment.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... INFRASTRUCTURE MANAGEMENT REAL-TIME SYSTEM MANAGEMENT INFORMATION PROGRAM Real-Time System Management Information... operated by the State. In addition, the real-time information program shall complement current... 23 Highways 1 2013-04-01 2013-04-01 false Real-time information program establishment. 511.311...

  14. 23 CFR 511.311 - Real-time information program establishment.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... INFRASTRUCTURE MANAGEMENT REAL-TIME SYSTEM MANAGEMENT INFORMATION PROGRAM Real-Time System Management Information... operated by the State. In addition, the real-time information program shall complement current... 23 Highways 1 2012-04-01 2012-04-01 false Real-time information program establishment. 511.311...

  15. Neoplastic transformation of SV40-immortalized human urinary tract epithelial cells by in vitro exposure to 3-methylcholanthrene.

    PubMed

    Reznikoff, C A; Loretz, L J; Christian, B J; Wu, S Q; Meisner, L F

    1988-08-01

    Normal human urinary tract epithelial cells (HUC) were neoplastically transformed in vitro using a step-wise strategy. First, a partially transformed non-virus-producing cell line was obtained after infection of HUC with simian virus 40 (SV40). This cell line (SV-HUC-1) was demonstrated to be clonal in origin, as 100% of cells contained at least five of seven marker chromosomes. Marker chromosomes were formed by balanced translocations resulting in a 'pseudodiploid' cell line. SV-HUC-1 showed altered growth properties in vitro (e.g. anchorage independent growth) but failed to form tumors in athymic nude mice, even after 3 years in culture (80 passages). In the studies reported here, SV-HUC-1 at early passages (P15-P19) were exposed to 3-methylcholanthrene (MCA) in three separate experiments. After a six-week post-treatment period of cell culture, cells were inoculated s.c. into athymic nude mice. In all experiments, MCA-treated SV-HUC-1 formed carcinomas in mice usually with a latent period of 5-8 weeks. These carcinomas showed heterogeneity with respect to histopathologies and growth properties in the mice and karyotypes. All the tumors retained SV-HUC-1 chromosome markers, but each independent transformant was aneuploid and contained unique new marker chromosomes. Chromosomes usually altered in tumor cells included numbers 3, 5, 6, 9, 11 and 13. Mutations in the ras family of cellular proto-oncogenes resulting in altered mobility of the p21 protein product were not detected in six cell lines established from independently derived tumors. It is not yet known whether other cellular proto-oncogenes are activated in these tumorigenic transformants. Neither control SV-HUC-1 (which were not exposed to MCA), nor early passage HUC exposed to MCA formed tumors when inoculated into mice. Thus, the tumorigenic transformation of HUC resulted from the combined actions of SV40 and MCA.

  16. Structural and relaxor-like dielectric properties of unfilled tungsten bronzes Ba{sub 5−5x}Sm{sub 5x}Ti{sub 5x}Nb{sub 10−5x}O{sub 30}

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wei, T., E-mail: weitong.nju@gmail.com, E-mail: weitong-nju@163.com; Dong, Z.; Zhou, Q. J.

    2016-03-28

    New unfilled tetragonal tungsten bronze (TTB) oxides, Ba{sub 5−5x}Sm{sub 5x}Ti{sub 5x}Nb{sub 10−5x}O{sub 30} (BSTN-x), where 0.10 ≤ x ≤ 0.35, have been synthesized in this work. Their crystal structure was determined and analyzed based on Rietveld structural refinement. It is found that single TTB phase can be formed in a particular x range (i.e., 0.15 ≤ x ≤ 0.3) due to the competition interaction between tolerance factor and electronegativity difference. Furthermore, dielectric and ferroelectric results indicate that phase transitions and ferroelectric states are sensitive to x. Referring to the local chemistry, we suggest that the raise of vacancies at the A{sub 2}-site compared with that of A{sub 1}-sitemore » will intensely depress the normal ferroelectric phase and is in favor of relaxor ferroelectric state. Macroscopically, previous A-site size difference standpoint on fill TTB compounds cannot give a reasonable explanation about the variation of dielectric maximum temperature (T{sub m}) for present BSTN-x compounds. Alternatively, tetragonality (c/a) is adopted which can well describe the variation of T{sub m} in whole x range. In addition, one by one correspondence between tetragonality and electrical features can be found, and the compositions involving high c/a are usually stabilized in normal ferroelectric phase. It is believed that c/a is a more appropriate parameter to illustrate the variation of ferroelectric properties for unfilled TTB system.« less

  17. 23 CFR 627.5 - Applicable projects.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Applicable projects. 627.5 Section 627.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS VALUE ENGINEERING § 627.5 Applicable projects. (a) A VE analysis shall be conducted prior to the completion of final...

  18. 23 CFR 627.5 - Applicable projects.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Applicable projects. 627.5 Section 627.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS VALUE ENGINEERING § 627.5 Applicable projects. (a) A VE analysis shall be conducted prior to the completion of final...

  19. Navy CG(X) Cruiser Program: Background, Oversight Issues, and Options for Congress

    DTIC Science & Technology

    2009-11-20

    and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK...Another press report (Katherine McIntire Peters, “Navy’s Top Officer Sees Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September...whether the schedule for procuring CG(X)s is properly aligned with foreign-country ballistic missile development programs. A 2005 defense trade

  20. Navy CG(X) Cruiser Program: Background, Oversight Issues, and Options for Congress

    DTIC Science & Technology

    2009-10-21

    and Options for Congress 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK...Navy’s Top Officer Sees Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September 24, 2008) quoted Admiral Gary Roughead, the...related question is whether the schedule for procuring CG(X)s is properly aligned with foreign-country ballistic missile development programs. A 2005

  1. 28 CFR 545.23 - Inmate work/program assignment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Inmate work/program assignment. 545.23... involvement is mandated by Bureau policy or statute (for example, the Literacy Program). Where such participation is not required by either policy or statute, exception may be made to allow an inmate to...

  2. Iron and fibroblast growth factor 23 in X-linked hypophosphatemia

    PubMed Central

    Imel, Erik A.; Gray, Amie; Padgett, Leah; Econs, Michael J.

    2014-01-01

    Background Excess fibroblast growth factor 23 (FGF23) causes hypophosphatemia in autosomal dominant hypophosphatemic rickets (ADHR) and X-linked hypophosphatemia (XLH). Iron status influences C-terminal FGF23 (incorporating fragments plus intact FGF23) in ADHR and healthy subjects, and intact FGF23 in ADHR. We hypothesized that in XLH serum iron would inversely correlate to C-terminal FGF23, but not to intact FGF23, mirroring the relationships in normal controls. Methods Subjects included 25 untreated outpatients with XLH at a tertiary medical center and 158 healthy adult controls. Serum iron and plasma intact FGF23 and C-terminal FGF23 were measured in stored samples. Results Intact FGF23 was greater than the control mean in 100% of XLH patients, and >2SD above the control mean in 88%, compared to 71% and 21% respectively for C-terminal FGF23. In XLH, iron correlated negatively to log-C-terminal FGF23 (r= −0.523, p<0.01), with a steeper slope than in controls (p<0.001). Iron was not related to log-intact FGF23 in either group. The log-ratio of intact FGF23 to C-terminal FGF23 was higher in XLH (0.00 ± 0.44) than controls (−0.28 ± 0.21, p<0.01), and correlated positively to serum iron (controls r= 0.276, p<0.001; XLH r= 0.428, p<0.05), with a steeper slope in XLH (p<0.01). Conclusion Like controls, serum iron in XLH is inversely related to C-terminal FGF23 but not intact FGF23. XLH patients are more likely to have elevated intact FGF23 than C-terminal FGF23. The relationships of iron to FGF23 in XLH suggest altered regulation of FGF23 cleaving may contribute to maintaining hypophosphatemia around an abnormal set-point. PMID:24325979

  3. Evolution of Eu valence and superconductivity in layered Eu0.5La0.5FBiS2 -xSex system

    NASA Astrophysics Data System (ADS)

    Mizuguchi, Y.; Paris, E.; Wakita, T.; Jinno, G.; Puri, A.; Terashima, K.; Joseph, B.; Miura, O.; Yokoya, T.; Saini, N. L.

    2017-02-01

    We have studied the effect of Se substitution on Eu valence in a layered Eu0.5La0.5FBiS2 -xSex superconductor using a combined analysis of x-ray absorption near-edge structure (XANES) and x-ray photoelectron spectroscopy (XPS) measurements. Eu L3-edge XANES spectra reveal that Eu is in the mixed valence state with coexisting Eu2 + and Eu3 +. The average Eu valence decreases sharply from ˜2.3 for x =0.0 to ˜2.1 for x =0.4 . Consistently, Eu 3 d XPS shows a clear decrease in the average valence by Se substitution. Bi 4 f XPS indicates that effective charge carriers in the BiCh2 (Ch = S, Se) layers are slightly increased by Se substitution. On the basis of the present results it has been discussed that the metallic character induced by Se substitution in Eu0.5La0.5FBiS2 -xSex is likely to be due to increased in-plane orbital overlap driven by reduced in-plane disorder that affects the carrier mobility.

  4. 23 CFR 1206.5 - Review process.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Review process. 1206.5 Section 1206.5 Highways NATIONAL... GUIDELINES RULES OF PROCEDURE FOR INVOKING SANCTIONS UNDER THE HIGHWAY SAFETY ACT OF 1966 § 1206.5 Review process. (a) In any fiscal year, if the Administrators determine, based on a preliminary review, that a...

  5. A direct estimate of poleward volume, heat, and freshwater fluxes at 59.5°N between Greenland and Scotland

    NASA Astrophysics Data System (ADS)

    Rossby, T.; Reverdin, Gilles; Chafik, Leon; Søiland, Henrik

    2017-07-01

    The meridional overturning circulation (MOC) in the North Atlantic plays a major role in the transport of heat from low to high latitudes. In this study, we combine recent measurements of currents from the surface to >700 m from a shipboard acoustic Doppler current profiler with Argo profiles (to 2000 m) to estimate poleward volume, heat, and freshwater flux at 59.5°N between Greenland and Scotland. This is made possible thanks to the vessel Nuka Arctica that operates on a 3 week schedule between Greenland and Denmark. For the period late 2012 to early 2016, the deseasoned mean meridional overturning circulation reaches a 18.4 ± 3.4 Sv maximum at the σθ = 27.55 kg m-3 isopycnal, which varies in depth from near the surface in the western Irminger Sea to 1000 m in Rockall Trough. The total heat and freshwater fluxes across 59.5°N = 399 ± 74 TW and -0.20 ± 0.04 Sv, where the uncertainties are principally due to that of the MOC. Analysis of altimetric sea surface height variations along exactly the same route reveals a somewhat stronger geostrophic flow north during this period compared to the 23 year mean suggesting that for a long-term mean the above flux estimates should be reduced slightly to 17.4 Sv, 377 TW, and -0.19 Sv, respectively, with the same estimate uncertainties. The ADCP program is ongoing.

  6. NMR and X-ray studies of isomeric 22,23-dihydroxy stigmastanes

    NASA Astrophysics Data System (ADS)

    Khripach, Vladimir A.; Zhabinskii, Vladimir N.; Ivanova, Galina V.; Fando, Galina P.; Tsavlovskii, Dmitrii V.; Khripach, Natalya B.; Lyakhov, Alexander S.; Misharin, Alexander Yu.

    2010-06-01

    A comparative conformational study of steroidal side chain of (22 R,23 R)- and (22 S,23 S)-dihydroxy stigmastane derivatives was performed using single crystal X-ray diffraction and NMR spectroscopy. The preferred conformation in solution was shown to be close to that in the crystal. (22 R,23 R)-Isomers typical for natural plant steroid hormones brassinosteroids adopt a conformation in which both hydroxyl groups are pointed toward unhindered α-side of the steroidal plane and can thus participate in biochemical processes. Unnatural (22 S,23 S)-counterparts exhibit a conformation with the two hydroxyl groups oriented in the opposite direction and sterically hindered by 21-methyl group and terminal side chain fragment.

  7. Botulinum Neurotoxin Serotype A Recognizes Its Protein Receptor SV2 by a Different Mechanism than Botulinum Neurotoxin B Synaptotagmin

    PubMed Central

    Weisemann, Jasmin; Stern, Daniel; Mahrhold, Stefan; Dorner, Brigitte G.; Rummel, Andreas

    2016-01-01

    Botulinum neurotoxins (BoNTs) exhibit extraordinary potency due to their exquisite neurospecificity, which is achieved by dual binding to complex polysialo-gangliosides and synaptic vesicle proteins. The luminal domain 4 (LD4) of the three synaptic vesicle glycoprotein 2 isoforms, SV2A‐C, identified as protein receptors for the most relevant serotype BoNT/A, binds within the 50 kDa cell binding domain HC of BoNT/A. Here, we deciphered the BoNT/A‐SV2 interactions in more detail. In pull down assays, the binding of HCA to SV2-LD4 isoforms decreases from SV2C >> SV2A > SV2B. A binding constant of 200 nM was determined for BoNT/A to rat SV2C-LD4 in GST pull down assay. A similar binding constant was determined by surface plasmon resonance for HCA to rat SV2C and to human SV2C, the latter being slightly lower due to the substitution L563F in LD4. At pH 5, as measured in acidic synaptic vesicles, the binding constant of HCA to hSV2C is increased more than 10-fold. Circular dichroism spectroscopy reveals that the quadrilateral helix of SV2C-LD4 already exists in solution prior to BoNT/A binding. Hence, the BoNT/A‐SV2C interaction is of different nature compared to BoNT/B‐Syt-II. In particular, the preexistence of the quadrilateral β-sheet helix of SV2 and its pH-dependent binding to BoNT/A via backbone–backbone interactions constitute major differences. Knowledge of the molecular details of BoNT/A‐SV2 interactions drives the development of high affinity peptides to counteract BoNT/A intoxications or to capture functional BoNT/A variants in innovative detection systems for botulism diagnostic. PMID:27196927

  8. Synaptic vesicle glycoprotein 2A (SV2A) regulates kindling epileptogenesis via GABAergic neurotransmission

    PubMed Central

    Tokudome, Kentaro; Okumura, Takahiro; Shimizu, Saki; Mashimo, Tomoji; Takizawa, Akiko; Serikawa, Tadao; Terada, Ryo; Ishihara, Shizuka; Kunisawa, Naofumi; Sasa, Masashi; Ohno, Yukihiro

    2016-01-01

    Synaptic vesicle glycoprotein 2A (SV2A) is a prototype synaptic vesicle protein regulating action potential-dependent neurotransmitters release. SV2A also serves as a specific binding site for certain antiepileptics and is implicated in the treatment of epilepsy. Here, to elucidate the role of SV2A in modulating epileptogenesis, we generated a novel rat model (Sv2aL174Q rat) carrying a Sv2a-targeted missense mutation (L174Q) and analyzed its susceptibilities to kindling development. Although animals homozygous for the Sv2aL174Q mutation exhibited normal appearance and development, they are susceptible to pentylenetetrazole (PTZ) seizures. In addition, development of kindling associated with repeated PTZ treatments or focal stimulation of the amygdala was markedly facilitated by the Sv2aL174Q mutation. Neurochemical studies revealed that the Sv2aL174Q mutation specifically reduced depolarization-induced GABA, but not glutamate, release in the hippocampus without affecting basal release or the SV2A expression level in GABAergic neurons. In addition, the Sv2aL174Q mutation selectively reduced the synaptotagmin1 (Syt1) level among the exocytosis-related proteins examined. The present results demonstrate that dysfunction of SV2A due to the Sv2aL174Q mutation impairs the synaptic GABA release by reducing the Syt1 level and facilitates the kindling development, illustrating the crucial role of SV2A-GABA system in modulating kindling epileptogenesis. PMID:27265781

  9. 48 CFR 23.404 - Agency affirmative procurement programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... REGULATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Use of Recovered Materials and Biobased Products 23.404 Agency...

  10. 48 CFR 23.404 - Agency affirmative procurement programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... REGULATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Use of Recovered Materials and Biobased Products 23.404 Agency...

  11. Synchrotron powder X-ray diffraction and structural analysis of Eu0.5La0.5FBiS2-x Se x

    NASA Astrophysics Data System (ADS)

    Nagasaka, K.; Jinno, G.; Miura, O.; Miura, A.; Moriyoshi, C.; Kuroiwa, Y.; Mizuguchi, Y.

    2017-07-01

    Eu0.5La0.5FBiS2-x Se x is a new BiS2-based superconductor system. In Eu0.5La0.5FBiS2-x Se x , electron carriers are doped to the BiS2 layer by the substitution of Eu by La. Bulk superconductivity in this system is induced by increasing the in-plane chemical pressure, which is controlled by the Se concentration (x). In this study, we have analysed the crystal structure of Eu0.5La0.5FBiS2-x Se x using synchrotron powder diffraction and the Rietveld refinement. The precise determination of the structural parameters and thermal factors suggest that the emergence of bulk superconductivity in Eu0.5La0.5FBiS2-x Se x is achieved by the enhanced in-plane chemical pressure and the decrease in in-plane disorder.

  12. Analysis of plasma membrane Ca(2+)-ATPase expression in control and SV40-transformed human fibroblasts.

    PubMed

    Reisner, P D; Brandt, P C; Vanaman, T C

    1997-01-01

    It has been long known that neoplastic transformation is accompanied by a lowered requirement for extracellular Ca2+ for growth. The studies presented here demonstrate that human fibroblastic cell lines produce the two commonly found 'housekeeping' isoforms of the plasma membrane Ca(2+)-ATPase (PMCA), PMCA1b and 4b, and at the expression of both is demonstrably lower in cell lines neoplastically transformed by SV40 than in the corresponding parental cell lines. Western blot analyses of lysates from control (GM00037) and SV40-transformed (GM00637) skin fibroblasts revealed a 138 kDa PMCA whose level was significantly lower in the SV40-transformed cells relative to either total cellular protein or alpha-tubulin. Similar analyses of plasma membrane preparations from control WI-38) and SV40-transformed (WI-38VA13) lung fibroblasts revealed 3-4-fold lower levels of PMCA in the SV40-transformed cells. Competitive ELISAs performed on detergent solubilized plasma membrane preparations indicated at least 3-4-fold lower levels of PMCA in the SV40-transformed cell lines compared to controls. Reverse transcriptase coupled-PCR analyses showed that PMCA1b and PMCA4b were the only isoforms expressed in all four cell lines. The PMCA4b mRNA level detected by Northern analysis also was substantially lower in SV40 transformed skin fibroblasts than in non-transformed fibroblasts. Quantitative RT-PCR analyses showed levels of PMCA1b and 4b mRNAs to be 5 and 10-fold lower, respectively, in GM00637 than in GM00037 when the levels of PCR products were normalized to glyceraldehyde-3-phosphate dehydrogenase (G3PDH) mRNA. These results demonstrate that the expression of these distinct PMCA genes is substantially lower in SV40 transformed human skin and lung fibroblasts and may be coordinately regulated in these cells.

  13. Apollo lunar orbital sciences program alpha and X-ray spectrometers

    NASA Technical Reports Server (NTRS)

    1972-01-01

    The development of the alpha and X-ray spectrometers which were used on the Apollo 15 and 16 flights is discussed. Specific subjects presented are: (1) lunar program management, (2) scientific and technical approach, (3) major test programs, (4) reliability, quality assurance, and safety, and (5) subcontract management.

  14. Navy Ohio Replacement (SSBN[X]) Ballistic Missile Submarine Program: Background and Issues for Congress

    DTIC Science & Technology

    2016-04-05

    Navy Ohio Replacement (SSBN[ X ]) Ballistic Missile Submarine Program: Background and Issues for Congress Ronald O’Rourke Specialist in Naval...Affairs April 5, 2016 Congressional Research Service 7-5700 www.crs.gov R41129 Navy Ohio Replacement (SSBN[ X ]) Ballistic Missile Submarine...as the SSBN( X ) program, as the Navy’s top priority program. The Navy wants to procure the first Ohio replacement boat in FY2021, and the $773.1

  15. Is it useful to assess annual effective doses that are less than 100 mSv?

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Strom, Daniel J.; Cameron, John R.

    It is useful to assess annual effective doses less than 100 mSv. Beyond the''score-keeping'' needs of regulatory compliance, there are at least seven other valid reasons for performing personnel monitoring, many of which fall into the category of''no news is good news,'' or more aptly,''null news, as long as you can prove it, is good news.'' These are performance measures for contractual compliance, diagnosis of problems with radiation protection programs, health surveillance and occupational epidemiology, prevention of and support for litigation, demonstration of management commitment and safety, worker counseling, ensuring peace of mind. Furthermore, it is shown that there ismore » very reasonable expectation that detriment may be associated with doses smaller than 100 mSv per year.« less

  16. Crystallization and preliminary X-ray analysis of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis

    PubMed Central

    Tamura, Haruka; Ashida, Hiroki; Koga, Shogo; Saito, Yohtaro; Yadani, Tomonori; Kai, Yasushi; Inoue, Tsuyoshi; Yokota, Akiho; Matsumura, Hiroyoshi

    2009-01-01

    2,3-Diketo-5-methylthiopentyl-1-phosphate enolase (DK-MTP-1P enolase) from Bacillus subtilis was crystallized using the hanging-drop vapour-diffusion method. Crystals grew using PEG 3350 as the precipitant at 293 K. The crystals diffracted to 2.3 Å resolution at 100 K using synchrotron radiation and were found to belong to the monoclinic space group P21, with unit-cell parameters a = 79.3, b = 91.5, c = 107.0 Å, β = 90.8°. The asymmetric unit contained four molecules of DK-MTP-1P enolase, with a V M value of 2.2 Å3 Da−1 and a solvent content of 43%. PMID:19194007

  17. [Neutron Dosimetry System Using CR-39 for High-energy X-ray Radiation Therapy].

    PubMed

    Yabuta, Kazutoshi; Monzen, Hajime; Tamura, Masaya; Tsuruta, Takao; Itou, Tetsuo; Nohtomi, Akihiro; Nishimura, Yasumasa

    2014-01-01

    Neutrons are produced during radiation treatment by megavolt X-ray energies. However, it is difficult to measure neutron dose especially just during the irradiation. Therefore, we have developed a system for measuring neutrons with the solid state track detector CR-39, which is free from the influence of the X-ray beams. The energy spectrum of the neutrons was estimated by a Monte Carlo simulation method, and the estimated neutron dose was corrected by the contribution ratio of each energy. Pit formation rates of CR-39 ranged from 2.3 x 10(-3) to 8.2 x 10(-3) for each detector studied. According to the estimated neutron energy spectrum, the energy values for calibration were 144 keV and 515keV, and the contribution ratios were approximately 40:60 for 10 MV photons and 20:70 for photons over 15 MV. Neutron doses measured in the center of a high-energy X-ray field were 0.045 mSv/Gy for a 10 MV linear accelerator and 0.85 mSv/Gy for a 20 MV linear accelerator. We successfully developed the new neutron dose measurement system using the solid track detector, CR-39. This on-time neutron measurement system allows users to measure neutron doses produced in the radiation treatment room more easily.

  18. Is the simian virus SV40 associated with idiopathic focal segmental glomerulosclerosis in humans?

    PubMed

    Galdenzi, Gabriella; Lupo, Antonio; Anglani, Franca; Perini, Marino; Galeazzi, Luciano; Giunta, Sergio; Marcantoni, Carmelita; Del Prete, Dorella; Graziotto, Romina; D'angelo, Angela; Maschio, Giuseppe; Gambaro, Giovanni

    2003-01-01

    Glomerulosclerosis was reported in mice transgenic for the simian polyomavirus SV40 early region that contains the transforming sequences encoding the SV40 large T-antigen (TAG). This was discovered when an SV40 epidemic occurred following the use of contaminated polio vaccines during 1955-1963, and led to investigations that showed an association between SV40 infection and tumors in humans. We investigated the possible association of SV40 infection and idiopathic focal segmental glomerulosclerosis (FSGS). The study was performed in 17 Bouin-fixed, paraffin-embedded renal biopsies from FSGS patients and 10 matched biopsies from patients with IgA glomerulonephritis; all patients had undergone polio vaccination in the early 1960s. Extracted DNA was polymerase chain reaction (PCR) amplified using SV.for3/SV.rev primers and GabE1/GabE2 primers; both sets of primers map in the region of SV40 TAG sequences, and amplify a fragment of respectively 105-bp and 135-bp. The biopsies considered were those in which the DNA was sufficiently intact to allow amplification of a fragment of 102-bp of the ApoE gene. Three FSGS and none of the IgA biopsies were positive for the SV.for3/SV.rev fragment. Conversely, amplification with GabE1/GabE2 primers did not lead to any specific product in either the IgA or FSGS biopsies. Restriction fragment length polymorphism and sequencing analyses revealed that the positive results obtained with the SV.for3/SV.rev primers were due to amplicons generated by multiple dimerization of forward and reverse primers. With the limited number of patients investigated, this study excludes the hypothesis that SV40 is associated with idiopathic FSGS.

  19. New Observations of Soft X-ray (0.5-5 keV) Solar Spectra

    NASA Astrophysics Data System (ADS)

    Caspi, A.; Woods, T. N.; Mason, J. P.; Jones, A. R.; Warren, H. P.

    2013-12-01

    The solar corona is the brightest source of X-rays in the solar system, and the X-ray emission is highly variable on many time scales. However, the actual solar soft X-ray (SXR) (0.5-5 keV) spectrum is not well known, particularly during solar quiet periods, as, with few exceptions, this energy range has not been systematically studied in many years. Previous observations include high-resolution but very narrow-band spectra from crystal spectrometers (e.g., Yohkoh/BCS), or integrated broadband irradiances from photometers (e.g., GOES/XRS, TIMED/XPS, etc.) that lack detailed spectral information. In recent years, broadband measurements with moderate energy resolution (~0.5-0.7 keV FWHM) were made by SphinX on CORONAS-Photon and SAX on MESSENGER, although they did not extend to energies below ~1 keV. We present observations of solar SXR emission obtained using new instrumentation flown on recent SDO/EVE calibration rocket underflights. The photon-counting spectrometer, a commercial Amptek X123 with a silicon drift detector and an 8 μm Be window, measures the solar disk-integrated SXR emission from ~0.5 to >10 keV with ~0.15 keV FWHM resolution and 1 s cadence. A novel imager, a pinhole X-ray camera using a cooled frame-transfer CCD (15 μm pixel pitch), Ti/Al/C filter, and 5000 line/mm Au transmission grating, images the full Sun in multiple spectral orders from ~0.1 to ~5 nm with ~10 arcsec/pixel and ~0.01 nm/pixel spatial and spectral detector scales, respectively, and 10 s cadence. These instruments are prototypes for future CubeSat missions currently being developed. We present new results of solar observations on 04 October 2013 (NASA sounding rocket 36.290). We compare with previous results from 23 June 2012 (NASA sounding rocket 36.286), during which solar activity was low and no signal was observed above ~4 keV. We compare our spectral and imaging measurements with spectra and broadband irradiances from other instruments, including SDO/EVE, GOES/XRS, TIMED

  20. PSHA in Israel by using the synthetic ground motions from simulated seismicity: the modified SvE procedure

    NASA Astrophysics Data System (ADS)

    Meirova, T.; Shapira, A.; Eppelbaum, L.

    2018-05-01

    In this study, we updated and modified the SvE approach of Shapira and van Eck (Nat Hazards 8:201-215, 1993) which may be applied as an alternative to the conventional probabilistic seismic hazard assessment (PSHA) in Israel and other regions of low and moderate seismicity where measurements of strong ground motions are scarce. The new computational code SvE overcomes difficulties associated with the description of the earthquake source model and regional ground-motion scaling. In the modified SvE procedure, generating suites of regional ground motion is based on the extended two-dimensional source model of Motazedian and Atkinson (Bull Seism Soc Amer 95:995-1010, 2005a) and updated regional ground-motion scaling (Meirova and Hofstteter, Bull Earth Eng 15:3417-3436, 2017). The analytical approach of Mavroeidis and Papageorgiou (Bull Seism Soc Amer 93:1099-1131, 2003) is used to simulate the near-fault acceleration with the near-fault effects. The comparison of hazard estimates obtained by using the conventional method implemented in the National Building Code for Design provisions for earthquake resistance of structures and the modified SvE procedure for rock-site conditions indicates a general agreement with some perceptible differences at the periods of 0.2 and 0.5 s. For the periods above 0.5 s, the SvE estimates are systematically greater and can increase by a factor of 1.6. For the soft-soil sites, the SvE hazard estimates at the period of 0.2 s are greater than those based on the CB2008 ground-motion prediction equation (GMPE) by a factor of 1.3-1.6. We suggest that the hazard estimates for the sites with soft-soil conditions calculated by the modified SvE procedure are more reliable than those which can be found by means of the conventional PSHA. This result agrees with the opinion that the use of a standard GMPE applying the NEHRP soil classification based on the V s, 30 parameter may be inappropriate for PSHA at many sites in Israel.

  1. Shielding properties of 80TeO2-5TiO2-(15-x) WO3-xAnOm glasses using WinXCom and MCNP5 code

    NASA Astrophysics Data System (ADS)

    Dong, M. G.; El-Mallawany, R.; Sayyed, M. I.; Tekin, H. O.

    2017-12-01

    Gamma ray shielding properties of 80TeO2-5TiO2-(15-x) WO3-xAnOm glasses, where AnOm is Nb2O5 = 0.01, 5, Nd2O3 = 3, 5 and Er2O3 = 5 mol% have been achieved. Shielding parameters; mass attenuation coefficients, half value layers, and macroscopic effective removal cross section for fast neutrons have been computed by using WinXCom program and MCNP5 Monte Carlo code. In addition, by using Geometric Progression method (G-P), exposure buildup factor values were also calculated. Variations of shielding parameters are discussed for the effect of REO addition into the glasses and photon energy.

  2. Environmental Radiation Measurements on the Mir Space Station. Program 1; Internal Experiment Program

    NASA Technical Reports Server (NTRS)

    Benton, E. V.; Frank, A. L.; Benton, E. R.

    1998-01-01

    As part of the NASA/Mir Phase 1B Science Program, the ionizing radiation environment inside and outside the Russian Mir's Space Station was monitored using a combination of Thermoluminescent Detectors (TLD) and CR-39 Plastic Nuclear Track Detectors (PNTD). Radiation measurements inside the Mir station were carried out using six Area Passive Dosimeters (APD), four located inside the Mir Base Block and two located inside the Kvant 2 module, during the NASA-2/Mir-21, NASA-3/Mir-22 and NASA-4/Mir-23 missions. The radiation environment under low shielding was measured using an External Dosimeter Array (EDA) mounted on the outer surface of the Kvant 2 module. The external radiation environment and a location inside the Kvant 2 roughly corresponding to the location of the EDA were monitored for 130 days during the NASA- 4/Mir-23 and NASA-5/Mir-24 missions. Dose rates measured by APD TLDs ranged from 271 to 407 microGy/d during the NASA-2/Mir-21 mission, from 265 to 378 microGy/d during the NASA-3/Mir-22 mission, and from 287 to 421 microGy/d during the NASA-4/Mir-23 mission. APD PNTDs have been analyzed and LET spectra have been Cenerated for the five APDs exposed on the NASA-2/Mir-21 mission and for two APD PNTDs exposed on the NASA-3/Mir-22 mission. Dose equivalent rates on the NASA-2/Mir-21 mission ranged from 513 microSv/d in the Kvant 2 module to 710 microSv/d on the floor of the Base Block. Dose as a function of shielding depth in TLDs has been measured in the thin TLD stacks including in the EDA. EDA dose range from 72.5 Gy under 0.0146 g/sq cm to 0.093 Gy under 3.25 g/sq cm of shielding. Readout and analysis of the reaming PNTDs form the NASA-3/Mir-22 mission and PNTDs from the NASA-4/Mir-23 mission (including those from the EDA) is ongoing and will be completed during the final year of this experiment. Dose equivalent rates for the NASA-3/Mir-22 and NASA-4/Mir-23 APDs will then be determined and comparisons will be made with both model calculations and with

  3. Endogenous opioid mechanisms partially mediate P2X3/P2X2/3-related antinociception in rat models of inflammatory and chemogenic pain but not neuropathic pain.

    PubMed

    McGaraughty, Steve; Honore, Prisca; Wismer, Carol T; Mikusa, Joseph; Zhu, Chang Z; McDonald, Heath A; Bianchi, Bruce; Faltynek, Connie R; Jarvis, Michael F

    2005-09-01

    P2X3/P2X2/3 receptors have emerged as important components of nociception. However, there is limited information regarding the neurochemical systems that are affected by antagonism of the P2X3/P2X2/3 receptor and that ultimately contribute to the ensuing antinociception. In order to determine if the endogenous opioid system is involved in this antinociception, naloxone was administered just prior to the injection of a selective P2X3/P2X2/3 receptor antagonist, A-317491, in rat models of neuropathic, chemogenic, and inflammatory pain. Naloxone (1-10 mg kg(-1), i.p.), dose-dependently reduced the antinociceptive effects of A-317491 (1-300 micromol kg(-1), s.c.) in the CFA model of thermal hyperalgesia and the formalin model of chemogenic pain (2nd phase), but not in the L5-L6 spinal nerve ligation model of neuropathic allodynia. In comparison experiments, the same doses of naloxone blocked or attenuated the actions of morphine (2 or 8 mg kg(-1), s.c.) in each of these behavioral models. Injection of a peripheral opioid antagonist, naloxone methiodide (10 mg kg(-1), i.p.), did not affect A-317491-induced antinociception in the CFA and formalin assays, suggesting that the opioid component of this antinociception occurred within the CNS. Furthermore, this utilization of the central opioid system could be initiated by antagonism of spinal P2X3/P2X2/3 receptors since the antinociceptive actions of intrathecally delivered A-317491 (30 nmol) in the formalin model were reduced by both intrathecally (10-50 nmol) and systemically (10 mg kg(-1), i.p.) administered naloxone. This utilization of the opioid system was not specific to A-317491 since suramin-, a nonselective P2X receptor antagonist, induced antinociception was also attenuated by naloxone. In in vitro studies, A-317491 (3-100 microM) did not produce any agonist response at delta opioid receptors expressed in NG108-15 cells. A-317491 had been previously shown to be inactive at the kappa and mu opioid receptors

  4. Colossal permittivity behavior and its origin in rutile (Mg1/3Ta2/3)xTi1-xO2.

    PubMed

    Dong, Wen; Chen, Dehong; Hu, Wanbiao; Frankcombe, Terry J; Chen, Hua; Zhou, Chao; Fu, Zhenxiao; Wei, Xiaoyong; Xu, Zhuo; Liu, Zhifu; Li, Yongxiang; Liu, Yun

    2017-08-30

    This work investigates the synthesis, chemical composition, defect structures and associated dielectric properties of (Mg 2+ , Ta 5+ ) co-doped rutile TiO 2 polycrystalline ceramics with nominal compositions of (Mg 2+ 1/3 Ta 5+ 2/3 ) x Ti 1-x O 2 . Colossal permittivity (>7000) with a low dielectric loss (e.g. 0.002 at 1 kHz) across a broad frequency/temperature range can be achieved at x = 0.5% after careful optimization of process conditions. Both experimental and theoretical evidence indicates such a colossal permittivity and low dielectric loss intrinsically originate from the intragrain polarization that links to the electron-pinned [Formula: see text] defect clusters with a specific configuration, different from the defect cluster form previously reported in tri-/pent-valent ion co-doped rutile TiO 2 . This work extends the research on colossal permittivity and defect formation to bi-/penta-valent ion co-doped rutile TiO 2 and elucidates a likely defect cluster model for this system. We therefore believe these results will benefit further development of colossal permittivity materials and advance the understanding of defect chemistry in solids.

  5. Individual external doses below the lowest reference level of 1 mSv per year five years after the 2011 Fukushima nuclear accident among all children in Soma City, Fukushima: A retrospective observational study.

    PubMed

    Tsubokura, Masaharu; Murakami, Michio; Nomura, Shuhei; Morita, Tomohiro; Nishikawa, Yoshitaka; Leppold, Claire; Kato, Shigeaki; Kami, Masahiro

    2017-01-01

    After the 2011 Fukushima Daiichi nuclear power plant accident, little information has been available on individual doses from external exposure among residents living in radioactively contaminated areas near the nuclear plant; in the present study we evaluated yearly changes in the doses from external exposure after the accident and the effects of decontamination on external exposure. This study considered all children less than 16 years of age in Soma City, Fukushima who participated in annual voluntary external exposure screening programs during the five years after the accident (n = 5,363). In total, 14,405 screening results were collected. The median participant age was eight years. The geometric mean levels of annual additional doses from external exposure attributable to the Fukushima accident, decreased each year: 0.60 mSv (range: not detectable (ND)-4.29 mSv), 0.37 mSv (range: ND-3.61 mSv), 0.22 mSv (range: ND-1.44 mSv), 0.20 mSv (range: ND-1.87 mSv), and 0.17 mSv (range: ND-0.85 mSv) in 2011, 2012, 2013, 2014, and 2015, respectively. The proportion of residents with annual additional doses from external exposure of more than 1 mSv dropped from 15.6% in 2011 to zero in 2015. Doses from external exposure decreased more rapidly than those estimated from only physical decay, even in areas without decontamination (which were halved in 395 days from November 15, 2011), presumably due to the weathering effects. While the ratios of geometric mean doses immediately after decontamination to before were slightly lower than those during the same time in areas without decontamination, annual additional doses reduced by decontamination were small (0.04-0.24 mSv in the year of immediately after decontamination was completed). The results of this study showed that the levels of external exposure among Soma residents less than 16 years of age decreased during the five years after the Fukushima Daiichi nuclear power plant accident. Decontamination had only limited and

  6. 25 CFR 36.23 - Standard VIII-Junior high/middle school instructional program.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... instructional program. (7) Physical education. One unit shall be required of each student in the junior high... program. 36.23 Section 36.23 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR EDUCATION MINIMUM ACADEMIC STANDARDS FOR THE BASIC EDUCATION OF INDIAN CHILDREN AND NATIONAL CRITERIA FOR DORMITORY...

  7. 23 CFR 752.5 - Safety rest areas.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Safety rest areas. 752.5 Section 752.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT LANDSCAPE AND ROADSIDE DEVELOPMENT § 752.5 Safety rest areas. (a) Safety rest areas should provide facilities reasonably necessary...

  8. 23 CFR 752.5 - Safety rest areas.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Safety rest areas. 752.5 Section 752.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT LANDSCAPE AND ROADSIDE DEVELOPMENT § 752.5 Safety rest areas. (a) Safety rest areas should provide facilities reasonably necessary...

  9. 23 CFR 752.5 - Safety rest areas.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Safety rest areas. 752.5 Section 752.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT LANDSCAPE AND ROADSIDE DEVELOPMENT § 752.5 Safety rest areas. (a) Safety rest areas should provide facilities reasonably necessary...

  10. 23 CFR 752.5 - Safety rest areas.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Safety rest areas. 752.5 Section 752.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT LANDSCAPE AND ROADSIDE DEVELOPMENT § 752.5 Safety rest areas. (a) Safety rest areas should provide facilities reasonably necessary...

  11. 23 CFR 752.5 - Safety rest areas.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Safety rest areas. 752.5 Section 752.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RIGHT-OF-WAY AND ENVIRONMENT LANDSCAPE AND ROADSIDE DEVELOPMENT § 752.5 Safety rest areas. (a) Safety rest areas should provide facilities reasonably necessary...

  12. Targeted Analysis of KLF6/KLF6-SV1 Regulating Pathways in Prostate Cancer Development and Metastasis

    DTIC Science & Technology

    2011-09-01

    and KLF6-SV1 it will be important to define the functionality of the putative NLS, the 5BR, as well as the role of nucleo-cytoplasmic shuttling in...aa EGFP -56 aa -16 aa KLF6 KLF6-SV1 5BR ZF,ZF~F3 ZF, ZF2 ZF3 l29KLF6 57KLF6 17KLF6 located near or within other important domains that...the transporter protein Crm1/Xpo1, first discovered in yeast [33–36]. Subcellular localization and protein turnover are two related events that are

  13. Dose evaluation for paediatric chest x-ray examinations in Brazil and Sudan: low doses and reliable examinations can be achieved in developing countries

    NASA Astrophysics Data System (ADS)

    Mohamadain, K. E. M.; da Rosa, L. A. R.; Azevedo, A. C. P.; Guebel, M. R. N.; Boechat, M. C. B.; Habani, F.

    2004-03-01

    Radiation protection in paediatric radiology deserves special attention since it is assumed that children are more sensitive to radiation than adults. The aim of this work is to estimate the entrance skin dose (ESD), the body organ dose (BOD) and the effective dose (E) for chest x-ray exposure of paediatric patients in five large units, three in Sudan and two in Brazil, and to compare the results obtained in both countries with each other and with other values obtained by some European countries. Two examination projections have been investigated, namely, postero-anterior (PA) and antero-posterior (AP). The age intervals considered were: 0-1 year, 1-5 years, 5-10 years and 10-15 years. The results have been obtained with the use of a software called DoseCal. Results of mean ESD for the age interval 1-5 years and AP projection are: 66 µGy (Instituto de Pediatria e Puericultura Martagão Gesteira—IPPMG Hospital), 41, 86 and 68 µGy (Instituto Fernandes Figueira—IFF Hospital), 161 µGy (Omdurman Hospital), 395 µGy (Khartoum Hospital) and 23 µGy (Ahmed Gasim Hospital). In the case of the IFF Hospital, the results refer, respectively, to rooms 1, 2 and for the six mobile equipments. The reference dose values given by the European Guidelines were exceeded in the Khartoum Hospital whilst in all the other hospitals results obtained were below CEC reference values and comparable with the results found in Sweden, Germany, Spain and Italy. The mean E for the same age interval was 11 µSv in the IPPMG, 6, 15 and 11 µSv in the IFF, respectively for rooms 1, 2 and the 6 mobiles, 25 µSv in the Omdurman Hospital, 45 µSv in the Khartoum Hospital and 3 µSv in the Ahmed Gasim Hospital. These are some examples of the large discrepancies that have been detected in this survey.

  14. The influence of SV40 immortalization of human fibroblasts on p53-dependent radiation responses

    NASA Technical Reports Server (NTRS)

    Kohli, M.; Jorgensen, T. J.

    1999-01-01

    The simian virus 40 large tumor antigen (SV40 Tag) has been ascribed many functions critical to viral propagation, including binding to the mammalian tumor suppressor p53. Recent studies have demonstrated that SV40-transformed murine cells have functional p53. The status of p53 in SV40-immortalized human cells, however, has not been characterized. We have found that in response to ionizing radiation, p53-dependent p21 transactivation activity is present, albeit reduced, in SV40-immortalized cells and that this activity can be further reduced with either dominant negative p53 expression or higher SV40 Tag expression. Furthermore, overexpression of p53 in SV40-immortalized ataxia-telangiectasia (A-T) cells restores p53-dependent p21 induction to typical A-T levels. All SV40-immortalized cell lines exhibited an absence of G1 arrest. Moreover, all SV40-immortalized cell lines exhibited increased apoptosis relative to primary cells in response to ionizing radiation, suggesting that SV40 immortalization results in a unique phenotype with regard to DNA damage responses. Copyright 1999 Academic Press.

  15. Dose inspection and risk assessment on radiation safety for the use of non-medical X-ray machines in Taiwan

    NASA Astrophysics Data System (ADS)

    Hsu, Fang-Yuh; Hsu, Shih-Ming; Chao, Jiunn-Hsing

    2017-11-01

    The subject of this study is the on-site visits and inspections of facilities commissioned by the Atomic Energy Council (AEC) in Taiwan. This research was conducted to evaluate the possible dose and dose rate of cabinet-type X-ray equipment with nominal voltages of 30-150 kV and open-beam (portable or handheld) equipment, taking both normal operation and possibly abnormal operation conditions into account. Doses and dose rates were measured using a plastic scintillation survey meter and an electronic personal dosimeter. In total, 401 X-ray machines were inspected, including 139 units with nominal voltages of 30-50 kV X-ray equipment, 140 units with nominal voltages of 50-150 kV, and 122 open-beam (portable or handheld) X-ray equipment. The investigated doses for radiation workers and non-radiation workers operating cabinet-type X-ray equipment under normal safety conditions were all at the background dose level. Several investigated dose rates at the position of 10 cm away from the surface of open-beam (portable or handheld) X-ray equipment were very high, such X-ray machines are used by aeronautical police for the detection of suspected explosives, radiation workers are far away (at least 10 m away) from the X-ray machine during its operation. The doses per operation in X-ray equipment with a 30-50 kV nominal voltage were less than 1 mSv in all cases of abnormal use. Some doses were higher than 1 mSv per operation for X-ray equipment of 50-150 kV nominal voltage X-ray. The maximum dose rates at the beam exit have a very wide range, mostly less than 100 μSv/s and the largest value is about 3.92 mSv/s for open-beam (portable or handheld) X-ray devices. The risk induced by operating X-ray devices with nominal voltages of 30-50 kV is extremely low. The 11.5 mSv dose due to one operation at nominal voltage of 50-150 kV X-ray device is equivalent to the exposure of taking 575 chest X-rays. In the abnormal use of open-beam (portable or handheld) X-ray equipment, the

  16. Commerical Crew Program - SpaceX

    NASA Image and Video Library

    2016-06-28

    The inter-stage of a SpaceX Falcon 9 rocket inside the company's manufacturing facility. SpaceX is developing its Crew Dragon spacecraft and Falcon 9 rocket in partnership with NASA's Commercial Crew Program to carry astronauts to and from the International Space Station.

  17. A new psychoactive 5H-2,3-benzodiazepine with a unique spectrum of activity.

    PubMed

    Horváth, K; Andrási, F; Berzsenyi, P; Pátfalusi, M; Patthy, M; Szabó, G; Sebestyén, L; Bagdy, E; Körösi, J; Botka, P

    1989-08-01

    The neuropharmacological effects of 1-(4-amino-phenyl)-4-methyl-7,8-dimethoxy-5H-2,3-benzodiazepine (GYKI 52 322) were investigated and compared with those of chlordiazepoxide and chlorpromazine. This novel 2,3-benzodiazepine displays neuroleptic activity in the apomorphine-climbing (ED50 = 1.15 mg/kg i.p.) and swim-induced grooming (ED50 = 6.9 mg/kg i.p.) tests in mice and it inhibits the conditioned avoidance response in rats (ED50 = 8.2 mg/kg i.p. and 9.8 mg/kg p.o.). However, it does not antagonize apomorphine-evoked vomiting in dogs; or stereotypy, hypermotility and turning in rats even at as high a dose as 50 mg/kg i.p. On the other hand it is active in the hole board test in mice (MED (minimal effective dose) = 0.5 mg/kg i.p.) and in the lick conflict assay in rats (MED = 5 mg/kg i.p.), indicating anxiolytic property. It shows antiaggressive effect in the fighting mice test (ED50 = 8.1 mg/kg p.o.) and the carbachol-rage procedure in cats (active at 10 mg/kg i.p.) According to the biochemical findings, this compound does not bind to the central dopamine receptors (IC50 greater than 10(-4) mol/l), but it shows affinity to the 5-HT1 receptors (IC50 = 7.1 x 10(-6) mol/l) and inhibits brain cAMP-phosphodiesterase (IC50 = 2.4 x 10(-5) mol/l). The substance causes no elevation of dopamine turnover and serum prolactin level suggesting fewer side effects. So the term "atypical neuroleptic agent" is proposed to characterize this molecule.

  18. Lactation-induced WAP-SV40 Tag transgene expression in C57BL/6J mice leads to mammary carcinoma.

    PubMed

    Hüsler, M R; Kotopoulis, K A; Sundberg, J P; Tennent, B J; Kunig, S V; Knowles, B B

    1998-07-01

    Two transgenic lineages were generated by directing the expression of SV40 T antigen to the mammary gland of inbred C57BL/6J mice using the whey acidic protein (WAP) promoter. In one lineage, WAPTag 1, multiparous female mice developed mammary adenocarcinoma with an average latency period of 13 months. The histopathological phenotype was heterogeneous, tumours occurred in a stochastic fashion, normal tissue was located next to neoplastic tissue, the mammary tumours usually developed and were remarkably similar to that observed in human cases. In addition, male and virgin females developed a poorly differentiated SV40 T antigen-positive soft tissue sarcoma, also at 13 months of age. In the other lineage, WAPTag 3, some parous females developed mammary tumours, but most mice succumbed to osteosarcomas arising from the os petrosum at 5.5 to 6 months of age and on necropsy, renal adenocarcinomas were also found. Appearance of these unexpected tumour types demonstrates the non-specific expression of SV40 Tag under the control of the WAP promoter. The expression of SV40 Tag in mammary glands at different stages of development was also examined, and only actively lactating glands were positive. This suggests that the abundant cyclic synthesis of SV40 Tag associated with pregnancy is required for mammary tumorigenesis in these lineages.

  19. Development, pilot testing and psychometric validation of a short version of the coronary artery disease education questionnaire: The CADE-Q SV.

    PubMed

    Ghisi, Gabriela Lima de Melo; Sandison, Nicole; Oh, Paul

    2016-03-01

    To develop, pilot test and psychometrically validate a shorter version of the coronary artery disease education questionnaire (CADE-Q), called CADE-Q SV. Based on previous versions of the CADE-Q, cardiac rehabilitation (CR) experts developed 20 items divided into 5 knowledge domains to comprise the first version of the CADE-Q SV. To establish content validity, they were reviewed by an expert panel (N=12). Refined items were pilot-tested in 20 patients, in which clarity was provided. A final version was generated and psychometrically-tested in 132CR patients. Test-retest reliability was assessed via the intraclass correlation coefficient (ICC), the internal consistency using Cronbach's alpha, and criterion validity with regard to patients' education and duration in CR. All ICC coefficients meet the minimum recommended standard. All domains were considered internally consistent (α>0.7). Criterion validity was supported by significant differences in mean scores by educational level (p<0.01) and duration in CR (p<0.05). Knowledge about exercise and nutrition was higher than knowledge about medical condition. The CADE-Q SV was demonstrated to have good reliability and validity. This is a short, quick and appropriate tool for application in clinical and research settings, assessing patients' knowledge during CR and as part of education programming. Copyright © 2015. Published by Elsevier Ireland Ltd.

  20. Navy CG(X) Cruiser Program: Background, Oversight Issues, and Options for Congress

    DTIC Science & Technology

    2008-11-18

    ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES... Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September 24, 2008) quoted Admiral Gary Roughead, the Chief of Naval Operations, as...the schedule for procuring CG(X)s is properly aligned with foreign-country ballistic missile development programs. A 2005 defense trade press report

  1. NASA's Hyper-X Program

    NASA Technical Reports Server (NTRS)

    Rausch, Vincent L.; McClinton, Charles R.; Sitz, Joel; Reukauf, Paul

    2000-01-01

    This paper provides an overview of the objectives and status of the Hyper-X program which is tailored to move hypersonic, airbreathing vehicle technology from the laboratory environment to the flight environment, the last stage preceding prototype development. The first Hyper-X research vehicle (HXRV), designated X-43, is being prepared at the Dryden Flight Research Center for flight at Mach 7 in the near future. In addition, the associated booster and vehicle-to-booster adapter are being prepared for flight and flight test preparations are well underway. Extensive risk reduction activities for the first flight and non-recurring design for the Mach 10 X-43 (3rd flight) are nearing completion. The Mach 7 flight of the X-43 will be the first flight of an airframe-integrated scramjet-powered vehicle.

  2. Commerical Crew Program - SpaceX

    NASA Image and Video Library

    2016-04-25

    The interior structure of the SpaceX Crew Dragon spacecraft at the company's facility in Hawthorne, California. SpaceX is developing its Crew Dragon spacecraft and Falcon 9 rocket in partnership with NASA’s Commercial Crew Program to carry astronauts to and from the International Space Station.

  3. Commerical Crew Program - SpaceX

    NASA Image and Video Library

    2016-04-25

    A technician works on the interior structure of the SpaceX Crew Dragon spacecraft at the company's facility in Hawthorne, California. SpaceX is developing its Crew Dragon in partnership with NASA’s Commercial Crew Program to carry astronauts to and from the International Space Station.

  4. SvABA: genome-wide detection of structural variants and indels by local assembly.

    PubMed

    Wala, Jeremiah A; Bandopadhayay, Pratiti; Greenwald, Noah F; O'Rourke, Ryan; Sharpe, Ted; Stewart, Chip; Schumacher, Steve; Li, Yilong; Weischenfeldt, Joachim; Yao, Xiaotong; Nusbaum, Chad; Campbell, Peter; Getz, Gad; Meyerson, Matthew; Zhang, Cheng-Zhong; Imielinski, Marcin; Beroukhim, Rameen

    2018-04-01

    Structural variants (SVs), including small insertion and deletion variants (indels), are challenging to detect through standard alignment-based variant calling methods. Sequence assembly offers a powerful approach to identifying SVs, but is difficult to apply at scale genome-wide for SV detection due to its computational complexity and the difficulty of extracting SVs from assembly contigs. We describe SvABA, an efficient and accurate method for detecting SVs from short-read sequencing data using genome-wide local assembly with low memory and computing requirements. We evaluated SvABA's performance on the NA12878 human genome and in simulated and real cancer genomes. SvABA demonstrates superior sensitivity and specificity across a large spectrum of SVs and substantially improves detection performance for variants in the 20-300 bp range, compared with existing methods. SvABA also identifies complex somatic rearrangements with chains of short (<1000 bp) templated-sequence insertions copied from distant genomic regions. We applied SvABA to 344 cancer genomes from 11 cancer types and found that short templated-sequence insertions occur in ∼4% of all somatic rearrangements. Finally, we demonstrate that SvABA can identify sites of viral integration and cancer driver alterations containing medium-sized (50-300 bp) SVs. © 2018 Wala et al.; Published by Cold Spring Harbor Laboratory Press.

  5. Impairment of Release Site Clearance within the Active Zone by Reduced SCAMP5 Expression Causes Short-Term Depression of Synaptic Release.

    PubMed

    Park, Daehun; Lee, Unghwi; Cho, Eunji; Zhao, Haiyan; Kim, Jung Ah; Lee, Byoung Ju; Regan, Philip; Ho, Won-Kyung; Cho, Kwangwook; Chang, Sunghoe

    2018-03-20

    Despite being a highly enriched synaptic vesicle (SV) protein and a candidate gene for autism, the physiological function of SCAMP5 remains mostly enigmatic. Here, using optical imaging and electrophysiological experiments, we demonstrate that SCAMP5 plays a critical role in release site clearance at the active zone. Truncation analysis revealed that the 2/3 loop domain of SCAMP5 directly interacts with adaptor protein 2, and this interaction is critical for its role in release site clearance. Knockdown (KD) of SCAMP5 exhibited pronounced synaptic depression accompanied by a slower recovery of the SV pool. Moreover, it induced a strong frequency-dependent short-term depression of synaptic release, even under the condition of sufficient release-ready SVs. Super-resolution microscopy further proved the defects in SV protein clearance induced by KD. Thus, reduced expression of SCAMP5 may impair the efficiency of SV clearance at the active zone, and this might relate to the synaptic dysfunction observed in autism. Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

  6. 17 CFR 5.23 - Notice of bulk transfers and bulk liquidations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 1 2011-04-01 2011-04-01 false Notice of bulk transfers and bulk liquidations. 5.23 Section 5.23 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION OFF-EXCHANGE FOREIGN CURRENCY TRANSACTIONS § 5.23 Notice of bulk transfers and bulk liquidations...

  7. 5 CFR 1312.23 - Access to classified information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Access to classified information. 1312.23 Section 1312.23 Administrative Personnel OFFICE OF MANAGEMENT AND BUDGET OMB DIRECTIVES CLASSIFICATION... Secret material, and an SF-705 for Confidential material. The cover sheet should be removed prior to...

  8. 5 CFR 1312.23 - Access to classified information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Access to classified information. 1312.23 Section 1312.23 Administrative Personnel OFFICE OF MANAGEMENT AND BUDGET OMB DIRECTIVES CLASSIFICATION... Secret material, and an SF-705 for Confidential material. The cover sheet should be removed prior to...

  9. 5 CFR 1312.23 - Access to classified information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Access to classified information. 1312.23 Section 1312.23 Administrative Personnel OFFICE OF MANAGEMENT AND BUDGET OMB DIRECTIVES CLASSIFICATION... Secret material, and an SF-705 for Confidential material. The cover sheet should be removed prior to...

  10. 5 CFR 1312.23 - Access to classified information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Access to classified information. 1312.23 Section 1312.23 Administrative Personnel OFFICE OF MANAGEMENT AND BUDGET OMB DIRECTIVES CLASSIFICATION... Secret material, and an SF-705 for Confidential material. The cover sheet should be removed prior to...

  11. ANS hard X-ray experiment development program. [emission from X-ray sources

    NASA Technical Reports Server (NTRS)

    Parsignault, D.; Gursky, H.; Frank, R.; Kubierschky, K.; Austin, G.; Paganetti, R.; Bawdekar, V.

    1974-01-01

    The hard X-ray (HXX) experiment is one of three experiments included in the Dutch Astronomical Netherlands Satellite, which was launched into orbit on 30 August 1974. The overall objective of the HXX experiment is the detailed study of the emission from known X-ray sources over the energy range 1.5-30keV. The instrument is capable of the following measurements: (1) spectral content over the full energy range with an energy resolution of approximately 20% and time resolution down to 4 seconds; (2) source time variability down to 4 milliseconds; (3) silicon emission lines at 1.86 and 2.00keV; (4) source location to a limit of one arc minute in ecliptic latitude; and (5) spatial structure with angular resolution of the arc minutes. Scientific aspects of experiment, engineering design and implementation of the experiment, and program history are included.

  12. Commerical Crew Program - SpaceX

    NASA Image and Video Library

    2014-05-21

    A SpaceX SuperDraco engine is hot-fired at the company's test facility in McGregor, Texas. SpaceX is developing its Crew Dragon spacecraft and Falcon 9 rocket in partnership with NASA’s Commercial Crew Program to carry astronauts to and from the International Space Station.

  13. [Optimizing condition for oligofectamine-mediated SP1 decoy oligodeoxynucleotides transfection into SV-40-PED cells].

    PubMed

    Huang, Yabing; Xu, Jing; Chen, Song

    2007-08-01

    To determine the optimizing parameters in transfecting the SV-40-PED cells mediated by oligofectamine. With a change of Decoy oligodeoxynucleotides (ODNs)/oligofectamine in ratio and the transfection time, the uptake rate and the mean fluorescence intensity of SP1 ODNs in the SV-40-PED cells were measured by flow cytometry to evaluate the transfection efficiencies. 4 microl oligofectamine with different concentrations of ODNs(2.5, 5.0, 7.5, 10.0 and 12.5 microl) were put into 100 microl of DMEM without serum and antibiotics. the (SV-40-PED) cells were transfected after 20 min at room temperature. the final concentration of SP1 decay ODNs were 50,100, 150, 200 and 250 nmol/L. Transfection effieiency was detected at 26 h after transfection. The intracellular distribution of SP1 ODNs was determined with a fluorescence microscope. The lactate dehydrogenase (LDH) activity in the supernatant was measured to assess the cytotoxicity. The uptake of SP1 ODNs into the SV-40-PED cells was significantly improved by oligofectamine. The cell appearance did not change much in the groups of 50, 100 and 150 nmol/L. In the groups of 200 and 250 nmol/L, the cell reverted after being shrinked and altered to round. At 26 h after the transfection, there was no marked change in the cell form at the concentration of 250 nmol/L. There was floatation at 48 and 72 h after the transfection. Under the fluorescence microscope, we observed fluorescent materials distributed in the cell nucleus in the successfully-transferred groups. We could see the nucleoli clearly in the groups of 200 nmol/L and 250 nmol/L. There was a stronger fluorescence intensity with a higher concentration and the fluorescent materials gathered at the cell nucleus. At the final concentration of 250 nmol/L, the LDH level was 137.12+/-3.92 U/L in the 72-h group, which was significantly higher those that in the 26-h group (49.61+/-17.13 U/L) and the 48-h group (120.26 +/- 8.42 U/L) (P<0.01). At 26 h after the transfection

  14. 23 CFR 190.5 - Bonus project claims.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Bonus project claims. 190.5 Section 190.5 Highways... CONTROLLING OUTDOOR ADVERTISING ON THE INTERSTATE SYSTEM § 190.5 Bonus project claims. (a) The State may claim... limits and areas excluded from the claim and form FHWA-1175, for the one-half percent bonus claim. (b...

  15. 23 CFR 190.5 - Bonus project claims.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Bonus project claims. 190.5 Section 190.5 Highways... CONTROLLING OUTDOOR ADVERTISING ON THE INTERSTATE SYSTEM § 190.5 Bonus project claims. (a) The State may claim... limits and areas excluded from the claim and form FHWA-1175, for the one-half percent bonus claim. (b...

  16. 23 CFR 190.5 - Bonus project claims.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Bonus project claims. 190.5 Section 190.5 Highways... CONTROLLING OUTDOOR ADVERTISING ON THE INTERSTATE SYSTEM § 190.5 Bonus project claims. (a) The State may claim... limits and areas excluded from the claim and form FHWA-1175, for the one-half percent bonus claim. (b...

  17. 23 CFR 190.5 - Bonus project claims.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Bonus project claims. 190.5 Section 190.5 Highways... CONTROLLING OUTDOOR ADVERTISING ON THE INTERSTATE SYSTEM § 190.5 Bonus project claims. (a) The State may claim... limits and areas excluded from the claim and form FHWA-1175, for the one-half percent bonus claim. (b...

  18. 23 CFR 190.5 - Bonus project claims.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Bonus project claims. 190.5 Section 190.5 Highways... CONTROLLING OUTDOOR ADVERTISING ON THE INTERSTATE SYSTEM § 190.5 Bonus project claims. (a) The State may claim... limits and areas excluded from the claim and form FHWA-1175, for the one-half percent bonus claim. (b...

  19. 6 CFR 5.23 - Responses to requests for access to records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 6 Domestic Security 1 2010-01-01 2010-01-01 false Responses to requests for access to records. 5.23 Section 5.23 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY DISCLOSURE OF RECORDS AND INFORMATION Privacy Act § 5.23 Responses to requests for access to records. (a...

  20. Navy CG(X) Cruiser Program: Background, Oversight Issues, and Options for Congress

    DTIC Science & Technology

    2008-10-27

    ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER 5e . TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Congressional...report (Katherine McIntire Peters, “Navy’s Top Officer Sees Lessons in Shipbuilding Program Failures,” GovernmentExecutive.com, September 24, 2008) quoted...40 A related question is whether the schedule for procuring CG(X)s is properly aligned with foreign-country ballistic missile development programs. A

  1. PATIENT RADIATION DOSE FROM CHEST X-RAY EXAMINATIONS IN THE WEST BANK-PALESTINE.

    PubMed

    Lahham, Adnan; Issa, Ahlam; ALMasri, Hussein

    2018-02-01

    Radiation doses to patients resulting from chest X-ray examinations were evaluated in four medical centers in the West Bank and East Jerusalem-Palestine. Absorbed organ and effective doses were calculated for a total of 428 adult male and female patients by using commercially available Monte Carlo based softwares; CALDOSE-X5 and PCXMC-2.0, and hermaphrodite mathematical adult phantoms. Patients were selected randomly from medical records in the time period from November 2014 to February 2015. A database of surveyed patients and exposure factors has been established and includes: patient's height, weight, age, gender, X-ray tube voltage, electric current (mAs), examination projection (anterior posterior (AP), posterior anterior (PA), lateral), X-ray tube filtration thickness in each X-ray equipment, anode angle, focus to skin distance and X-ray beam size. The average absorbed doses in the whole body from different projections were: 0.06, 0.07 and 0.11 mGy from AP, PA and lateral projections, respectively. The average effective dose for all surveyed patients was 0.14 mSv for all chest X-ray examinations and projections in the four investigated medical centers. The effect of projection geometry was also investigated. The average effective doses for AP, PA and lateral projections were 0.14, 0.07 and 0.22 mSv, respectively. The collective effective dose estimated for the exposed population was ~60 man-mSv. © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Commerical Crew Program - SpaceX

    NASA Image and Video Library

    2018-01-02

    A SpaceX Merlin engine is on a test stand at the company's facility in McGregor, Texas. SpaceX is developing its Crew Dragon spacecraft and Falcon 9 rocket in partnership with NASA’s Commercial Crew Program to carry astronauts to and from the International Space Station.

  3. 32 CFR 199.23 - Special Supplemental Food Program.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... health care during critical times of growth and development, in order to prevent the occurrence of health..., integrated health care network. (26) Supplemental foods. Foods containing nutrients determined by nutritional... (CONTINUED) MISCELLANEOUS CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) § 199.23...

  4. 32 CFR 199.23 - Special Supplemental Food Program.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... health care during critical times of growth and development, in order to prevent the occurrence of health..., integrated health care network. (26) Supplemental foods. Foods containing nutrients determined by nutritional... (CONTINUED) MISCELLANEOUS CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) § 199.23...

  5. 32 CFR 199.23 - Special Supplemental Food Program.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... health care during critical times of growth and development, in order to prevent the occurrence of health..., integrated health care network. (26) Supplemental foods. Foods containing nutrients determined by nutritional... (CONTINUED) MISCELLANEOUS CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) § 199.23...

  6. 32 CFR 199.23 - Special Supplemental Food Program.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... health care during critical times of growth and development, in order to prevent the occurrence of health..., integrated health care network. (26) Supplemental foods. Foods containing nutrients determined by nutritional... (CONTINUED) MISCELLANEOUS CIVILIAN HEALTH AND MEDICAL PROGRAM OF THE UNIFORMED SERVICES (CHAMPUS) § 199.23...

  7. 49 CFR 1552.23 - Security awareness training programs.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... employee to identify— (i) Uniforms and other identification, if any are required at the flight school, for... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FLIGHT SCHOOLS Flight School Security Awareness Training § 1552.23 Security awareness training programs. (a) General. A flight...

  8. 49 CFR 1552.23 - Security awareness training programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... employee to identify— (i) Uniforms and other identification, if any are required at the flight school, for... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FLIGHT SCHOOLS Flight School Security Awareness Training § 1552.23 Security awareness training programs. (a) General. A flight...

  9. 49 CFR 1552.23 - Security awareness training programs.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... employee to identify— (i) Uniforms and other identification, if any are required at the flight school, for... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FLIGHT SCHOOLS Flight School Security Awareness Training § 1552.23 Security awareness training programs. (a) General. A flight...

  10. 49 CFR 1552.23 - Security awareness training programs.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... employee to identify— (i) Uniforms and other identification, if any are required at the flight school, for... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FLIGHT SCHOOLS Flight School Security Awareness Training § 1552.23 Security awareness training programs. (a) General. A flight...

  11. 49 CFR 1552.23 - Security awareness training programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... employee to identify— (i) Uniforms and other identification, if any are required at the flight school, for... SECURITY ADMINISTRATION, DEPARTMENT OF HOMELAND SECURITY CIVIL AVIATION SECURITY FLIGHT SCHOOLS Flight School Security Awareness Training § 1552.23 Security awareness training programs. (a) General. A flight...

  12. Viral microRNA effects on persistent infection of human lymphoid cells by polyomavirus SV40

    PubMed Central

    McNees, Adrienne L.; Harrigal, Lindsay J.; Kelly, Aoife; Minard, Charles G.; Wong, Connie

    2018-01-01

    Background Polyomaviruses, including simian virus 40 (SV40), display evidence of lymphotropic properties. This study analyzed the nature of SV40–human lymphocyte interactions in established cell lines and in primary lymphocytes. The effects of viral microRNA and the structure of the viral regulatory region on SV40 persistence were examined. Results SV40 DNA was maintained in infected B cell and myeloid cell lines during cell growth for at least 28 days. Limiting dilution analysis showed that low amounts of SV40 DNA (~2 copies per cell) were retained over time. Infected B cells remained viable and able to proliferate. Genome copies of the SV40 microRNA-null mutant persisted at higher levels than the DNA of wild-type viruses. Complex viral regulatory regions produced modestly higher DNA levels than simple regulatory regions. Viral large T-antigen protein was detected at low frequency and at low levels in infected B cells. Following infection of primary lymphocytes, SV40 DNA was detected in CD19+ B cells and CD14+ monocytes, but not in CD3+ T cells. Rescue attempts using either lysates of SV40-infected B lymphocytes, coculture of live cells, or infectious center assays all showed that replication-competent SV40 could be recovered on rare occasions. SV40 infections altered the expression of several B cell surface markers, with more pronounced changes following infections with the microRNA-null mutant. Conclusion These findings indicate that SV40 can establish persistent infections in human B lymphocytes. The cells retain low copy numbers of viral DNA; the infections are nonproductive and noncytolytic but can occasionally produce infectious virus. SV40 microRNA negatively regulates the degree of viral effects on B cells. Significance Lymphocytes may serve as viral reservoirs and may function to disseminate polyomaviruses to different tissues in a host. To our knowledge, this report is the first extensive analysis of viral microRNA effects on SV40 infection of human

  13. X-29 flight-research program

    NASA Technical Reports Server (NTRS)

    Putnam, T. W.

    1984-01-01

    The X-29A aircraft is the first manned, experimental high-performance aircraft to be fabricated and flown in many years. The approach for expanding the X-29 flight envelope and collecting research data is described including the methods for monitoring wind divergence, flutter, and aeroservoelastic coupling of the aerodynamic forces with the structure and the flight-control system. Examples of the type of flight data to be acquired are presented along with types of aircraft maneuvers that will be flown. A brief description of the program management structure is also presented and the program schedule is discussed.

  14. Ionic configuration of copper ferrimanganites Cu 0.5Mn xFe 2.5- xO 4

    NASA Astrophysics Data System (ADS)

    Lenglet, M.; Kasperek, J.; Hannoyer, B.; Lopitaux, J.; d'Huysser, A.; Tellier, J. C.

    1992-06-01

    Mössbauer spectrometry, neutron diffraction, XANES, and XPS have led to the determination of the cation distributions of the system Cu 0.5Mn xFe 2.5- xO 4 (0≤ x≤1.5). The three cations are present in both tetrahedral and octahedral sites, and the relative number of Fe ions on A- and B-sites remains nearly constant in the whole range of x. It appears that for x≤0.5 manganese is divalent and copper is in its two oxidation states. For x>0.5 copper and iron are respectively divalent and trivalent; the manganese is in +2 and +3 oxydation states.

  15. Phase transition in the (Li 0.5-( x/2) K 0.5-( x/2) Cs x) 2SO 4 system

    NASA Astrophysics Data System (ADS)

    Hamed, A. E.; El-Aziz, Y. M. Abd.; Madi, N. K.; Kassem, M. E.

    1995-12-01

    Phase transition in the (Li 0.5-( x/2) K 0.5-( x/2) Cs x) 2SO 4 system was studied by measuring the specific heat at constant pressure, C p, as a function of temperature in the temperature range 300-800 K. For non-zero values of X ( X = 0.2%, 0.5%, 1% and 2%) the critical behaviour of the phase transition was found to change considerably compared with that of X = 0 or pure LiKSO 4. The observed change in the phase transition with increase of Cs 2SO 4 content ( X) was accompanied by a decrease in the thermodynamic parameters: the value of the specific heat at the transition point (Δ C P) max, the transition temperature, T1, and the value of the energy of ordering. The results were interpreted within the Landau thermodynamic theory of the phase transition.

  16. 49 CFR 268.5 - Federal funding sources for the Maglev Deployment Program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Federal funding sources for the Maglev Deployment... TECHNOLOGY DEPLOYMENT PROGRAM Overview § 268.5 Federal funding sources for the Maglev Deployment Program. (a) Federal Maglev Funds. Section 322 of Title 23 provides for the following funds for the Maglev Deployment...

  17. 49 CFR 268.5 - Federal funding sources for the Maglev Deployment Program.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 4 2012-10-01 2012-10-01 false Federal funding sources for the Maglev Deployment... TECHNOLOGY DEPLOYMENT PROGRAM Overview § 268.5 Federal funding sources for the Maglev Deployment Program. (a) Federal Maglev Funds. Section 322 of Title 23 provides for the following funds for the Maglev Deployment...

  18. 49 CFR 268.5 - Federal funding sources for the Maglev Deployment Program.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Federal funding sources for the Maglev Deployment... TECHNOLOGY DEPLOYMENT PROGRAM Overview § 268.5 Federal funding sources for the Maglev Deployment Program. (a) Federal Maglev Funds. Section 322 of Title 23 provides for the following funds for the Maglev Deployment...

  19. 49 CFR 268.5 - Federal funding sources for the Maglev Deployment Program.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Federal funding sources for the Maglev Deployment... TECHNOLOGY DEPLOYMENT PROGRAM Overview § 268.5 Federal funding sources for the Maglev Deployment Program. (a) Federal Maglev Funds. Section 322 of Title 23 provides for the following funds for the Maglev Deployment...

  20. Identification and SAR of novel diaminopyrimidines. Part 1: The discovery of RO-4, a dual P2X(3)/P2X(2/3) antagonist for the treatment of pain.

    PubMed

    Carter, David S; Alam, Muzaffar; Cai, Haiying; Dillon, Michael P; Ford, Anthony P D W; Gever, Joel R; Jahangir, Alam; Lin, Clara; Moore, Amy G; Wagner, Paul J; Zhai, Yansheng

    2009-03-15

    P2X purinoceptors are ligand-gated ion channels whose endogenous ligand is ATP. Both the P2X(3) and P2X(2/3) receptor subtypes have been shown to play an important role in the regulation of sensory function and dual P2X(3)/P2X(2/3) antagonists offer significant potential for the treatment of pain. A high-throughput screen of the Roche compound collection resulted in the identification of a novel series of diaminopyrimidines; subsequent optimization resulted in the discovery of RO-4, a potent, selective and drug-like dual P2X(3)/P2X(2/3) antagonist.

  1. 16 CFR 23.5 - Misuse of the word “vermeil.”

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Misuse of the word âvermeil.â 23.5 Section 23.5 Commercial Practices FEDERAL TRADE COMMISSION GUIDES AND TRADE PRACTICE RULES GUIDES FOR THE JEWELRY, PRECIOUS METALS, AND PEWTER INDUSTRIES § 23.5 Misuse of the word “vermeil.” (a) It is unfair or...

  2. Discovery of simian virus 40 (SV40) and its relationship to poliomyelitis virus vaccines.

    PubMed

    Hilleman, M R

    1998-01-01

    Simian Virus 40 (SV40) was discovered in 1959 as a covert contaminant of poliovirus vaccines prepared using Macacus monkey renal cell cultures. This inapparent polyoma virus of monkeys was detected using Cercopithecus renal cell cultures and was eliminated from poliovaccines. There has been no evidence to implicate SV40 virus of vaccine origin in long- or short-term consequences in human subjects. Of importance, SV40 virus provided a new model for basic studies of viral pathogenesis and for cell transformation and neoplasia. Neoplastic transformation is fixed on the promiscuous binding of SV40 large T antigen to anti-oncogene cellular protein elements. SV40 also served as a valuable model for defining the immunology of virus-induced cancer and in its prevention and cure. Further, it has been a prime tool for elucidating the molecular details of eukaryotic cell processes. Numerous techniques now used in molecular biology were pioneered in the SV40 system. The SV40 promoter is commonly used in vector expression constructs and it has continued to be a model to develop new tools for site-specific mutagenesis. The virus has been critically important to studies in modern genetics and in molecular biology.

  3. 44 CFR 59.23 - Priorities for the sale of flood insurance under the regular program.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... flood insurance under the regular program. 59.23 Section 59.23 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood Insurance Program GENERAL PROVISIONS Eligibility Requirements § 59.23 Priorities for the...

  4. 44 CFR 59.23 - Priorities for the sale of flood insurance under the regular program.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... flood insurance under the regular program. 59.23 Section 59.23 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood Insurance Program GENERAL PROVISIONS Eligibility Requirements § 59.23 Priorities for the...

  5. 44 CFR 59.23 - Priorities for the sale of flood insurance under the regular program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... flood insurance under the regular program. 59.23 Section 59.23 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY INSURANCE AND HAZARD MITIGATION National Flood Insurance Program GENERAL PROVISIONS Eligibility Requirements § 59.23 Priorities for the...

  6. Individual external doses below the lowest reference level of 1 mSv per year five years after the 2011 Fukushima nuclear accident among all children in Soma City, Fukushima: A retrospective observational study

    PubMed Central

    Murakami, Michio; Nomura, Shuhei; Morita, Tomohiro; Nishikawa, Yoshitaka; Leppold, Claire; Kato, Shigeaki; Kami, Masahiro

    2017-01-01

    After the 2011 Fukushima Daiichi nuclear power plant accident, little information has been available on individual doses from external exposure among residents living in radioactively contaminated areas near the nuclear plant; in the present study we evaluated yearly changes in the doses from external exposure after the accident and the effects of decontamination on external exposure. This study considered all children less than 16 years of age in Soma City, Fukushima who participated in annual voluntary external exposure screening programs during the five years after the accident (n = 5,363). In total, 14,405 screening results were collected. The median participant age was eight years. The geometric mean levels of annual additional doses from external exposure attributable to the Fukushima accident, decreased each year: 0.60 mSv (range: not detectable (ND)–4.29 mSv), 0.37 mSv (range: ND–3.61 mSv), 0.22 mSv (range: ND–1.44 mSv), 0.20 mSv (range: ND–1.87 mSv), and 0.17 mSv (range: ND–0.85 mSv) in 2011, 2012, 2013, 2014, and 2015, respectively. The proportion of residents with annual additional doses from external exposure of more than 1 mSv dropped from 15.6% in 2011 to zero in 2015. Doses from external exposure decreased more rapidly than those estimated from only physical decay, even in areas without decontamination (which were halved in 395 days from November 15, 2011), presumably due to the weathering effects. While the ratios of geometric mean doses immediately after decontamination to before were slightly lower than those during the same time in areas without decontamination, annual additional doses reduced by decontamination were small (0.04–0.24 mSv in the year of immediately after decontamination was completed). The results of this study showed that the levels of external exposure among Soma residents less than 16 years of age decreased during the five years after the Fukushima Daiichi nuclear power plant accident. Decontamination had only

  7. Properties of the simian virus 40 (SV40) large T antigens encoded by SV40 mutants with deletions in gene A.

    PubMed Central

    Cole, C N; Tornow, J; Clark, R; Tjian, R

    1986-01-01

    The biochemical properties of the large T antigens encoded by simian virus 40 (SV40) mutants with deletions at DdeI sites in the SV40 A gene were determined. Mutant large T antigens containing only the first 138 to 140 amino acids were unable to bind to the SV40 origin of DNA replication as were large T antigens containing at their COOH termini 96 or 97 amino acids encoded by the long open reading frame located between 0.22 and 0.165 map units (m.u.). All other mutant large T antigens were able to bind to the SV40 origin of replication. Mutants with in-phase deletions at 0.288 and 0.243 m.u. lacked ATPase activity, but ATPase activity was normal in mutants lacking origin-binding activity. The 627-amino acid large T antigen encoded by dlA2465, with a deletion at 0.219 m.u., was the smallest large T antigen displaying ATPase activity. Mutant large T antigens with the alternate 96- or 97-amino acid COOH terminus also lacked ATPase activity. All mutant large T antigens were found in the nuclei of infected cells; a small amount of large T with the alternate COOH terminus was also located in the cytoplasm. Mutant dlA2465 belonged to the same class of mutants as dlA2459. It was unable to form plaques on CV-1p cells at 37 or 32 degrees C but could form plaques on BSC-1 monolayers at 37 degrees C but not at 32 degrees C. It was positive for viral DNA replication and showed intracistronic complementation with any group A mutant whose large T antigen contained a normal carboxyl terminus. These findings and those of others suggest that both DNA binding and ATPase activity are required for the viral DNA replication function of large T antigen, that these two activities must be located on the same T antigen monomer, and that these two activities are performed by distinct domains of the polypeptide. These domains are distinct and separable from the domain affected by the mutation of dlA2465 and indicate that SV40 large T antigen is made up of at least three separate functional

  8. Estimation of neutron dose equivalent at the mezzanine of the Advanced Light Source and the laboratory boundary using the ORNL program MORSE.

    PubMed

    Sun, R K

    1990-12-01

    To investigate the radiation effect of neutrons near the Advanced Light Source (ALS) at Lawrence Berkeley Laboratory (LBL) with respect to the neutron dose equivalents in nearby occupied areas and at the site boundary, the neutron transport code MORSE, from Oak Ridge National Laboratory (ORNL), was used. These dose equivalents result from both skyshine neutrons transported by air scattering and direct neutrons penetrating the shielding. The ALS neutron sources are a 50-MeV linear accelerator and its transfer line, a 1.5-GeV booster, a beam extraction line, and a 1.9-GeV storage ring. The most conservative total occupational-dose-equivalent rate in the center of the ALS mezzanine, 39 m from the ALS center, was found to be 1.14 X 10(-3) Sv y-1 per 2000-h "occupational" year, and the total environmental-dose-equivalent rate at the ALS boundary, 125 m from the ALS center, was found to be 3.02 X 10(-4) Sv y-1 per 8760-h calendar year. More realistic dose-equivalent rates, using the nominal (expected) storage-ring current, were calculated to be 1.0 X 10(-4) Sv y-1 and 2.65 X 10(-5) Sv y-1 occupational year and calendar year, respectively, which are much lower than the DOE reporting levels.

  9. High prevalence of SV40 infection in patients with nodal non-Hodgkin's lymphoma but not acute leukemia independent of contaminated polio vaccines in Taiwan.

    PubMed

    Chen, Po-Min; Yen, Chueh-Chuan; Yang, Muh-Hwa; Poh, Say-Bee; Hsiao, Liang-Tsai; Wang, Wei-Shu; Lin, Peng-Chan; Lee, Ming-Yang; Teng, Hao-Wei; Bai, Li-Yuan; Chu, Chiau-Jun; Chao, Shu-Chauo; Yang, An-Hang; Chiou, Tzeon-Jye; Liu, Jin-Hwang; Chao, Ta-Chung

    2006-01-01

    Recent studies have linked simian virus 40 (SV40) to non-Hodgkin's lymphoma (NHL), especially in countries in which people were exposed to contaminated polio vaccines prior to 1963. In Taiwan, nearly all children were not exposed to contaminated polio vaccine during this period; the relationship between SV40 infection and hematological malignancies is unclear and deserves to be studied. Using PCR amplification of SV40 large T antigen DNA, confirmed by Southern blot hybridization and sequence analysis, 91 frozen lymph nodes from NHL patients were examined. Thirteen (14.3 percent) showed positive for SV40. All other test samples, including diagnostic bone marrow from patients with acute leukemia, peripheral blood from 10 relatives of SV40 positive-patients and 91 age-matched normal volunteers, and 5 reactive hyperplastic lymphoid tissues, showed negative. These results may reflect that human-to-human transmission of SV40 is independent of contaminated polio vaccines; and SV40 is possibly associated with the development of NHL in Taiwan (p = 0.0001). Prospective studies are needed to determine the prevalence of SV40 infections in our and other human populations and to explore the means of transmission of the virus.

  10. One-shot photochemical synthesis of 5-(thiophen-3-yl)pyrano[2,3-c]chromen-2(3H)-ones from 3-propynyloxy-chromenones: a case of an intramolecular Paterno-Buchi reaction.

    PubMed

    Jindal, Pooja; Bhatia, Rimpy; Khullar, Sadhika; Mandal, Sanjay K; Kamboj, Ramesh C

    2014-03-01

    5-(Thiophen-3-yl)pyrano[2,3-c]chromen-2(3H)-ones (2), angular tricyclic compounds, were synthesized in significantly high yields through the photoinduced intramolecular coupling of the acetylenic group with the carbonyl centre in 3-(prop-2-ynyloxy)-2-(thiophen-3-yl)-4H-chromen-4-ones (1). This photoreaction is a case of an intramolecular Paterno-Buchi reaction and is unprecedented in 3-propynyloxy-chromenones. The structure of 2 has been determined by spectroscopic (FTIR, NMR and mass) and single crystal X-ray crystallographic studies.

  11. Upgrading to MARPLOT 5.x

    EPA Pesticide Factsheets

    MARPLOT 5.x versions include significant changes from both the previous 4.x versions and the 3.x versions. To ensure that your data is successfully transferred from your old MARPLOT, follow these instructions carefully.

  12. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUC.

    PubMed

    Arthur, Kelly K; Kendrick, Brent S; Gabrielson, John P

    2015-01-01

    The levels and types of aggregates present in protein biopharmaceuticals must be assessed during all stages of product development, manufacturing, and storage of the finished product. Routine monitoring of aggregate levels in biopharmaceuticals is typically achieved by size exclusion chromatography (SEC) due to its high precision, speed, robustness, and simplicity to operate. However, SEC is error prone and requires careful method development to ensure accuracy of reported aggregate levels. Sedimentation velocity analytical ultracentrifugation (SV-AUC) is an orthogonal technique that can be used to measure protein aggregation without many of the potential inaccuracies of SEC. In this chapter, we discuss applications of SV-AUC during biopharmaceutical development and how characteristics of the technique make it better suited for some applications than others. We then discuss the elements of a comprehensive analytical control strategy for SV-AUC. Successful implementation of these analytical control elements ensures that SV-AUC provides continued value over the long time frames necessary to bring biopharmaceuticals to market. © 2015 Elsevier Inc. All rights reserved.

  13. Effective Dose Calculation Program (EDCP) for the usage of NORM-added consumer product.

    PubMed

    Yoo, Do Hyeon; Lee, Jaekook; Min, Chul Hee

    2018-04-09

    The aim of this study is to develop the Effective Dose Calculation Program (EDCP) for the usage of Naturally Occurring Radioactive Material (NORM) added consumer products. The EDCP was developed based on a database of effective dose conversion coefficient and the Matrix Laboratory (MATLAB) program to incorporate a Graphic User Interface (GUI) for ease of use. To validate EDCP, the effective dose calculated with EDCP by manually determining the source region by using the GUI and that by using the reference mathematical algorithm were compared for pillow, waist supporter, eye-patch and sleeping mattress. The results show that the annual effective dose calculated with EDCP was almost identical to that calculated using the reference mathematical algorithm in most of the assessment cases. With the assumption of the gamma energy of 1 MeV and activity of 1 MBq, the annual effective doses of pillow, waist supporter, sleeping mattress, and eye-patch determined using the reference algorithm were 3.444 mSv year -1 , 2.770 mSv year -1 , 4.629 mSv year -1 , and 3.567 mSv year -1 , respectively, while those calculated using EDCP were 3.561 mSv year -1 , 2.630 mSv year -1 , 4.740 mSv year -1 , and 3.780 mSv year -1 , respectively. The differences in the annual effective doses were less than 5%, despite the different calculation methods employed. The EDCP can therefore be effectively used for radiation protection management in the context of the usage of NORM-added consumer products. Additionally, EDCP can be used by members of the public through the GUI for various studies in the field of radiation protection, thus facilitating easy access to the program. Copyright © 2018. Published by Elsevier Ltd.

  14. Monsanto Gives Washington U. $23.5 Million.

    ERIC Educational Resources Information Center

    Culliton, Barbara J.

    1982-01-01

    Reviews various provisions of a five-year, $23.5-million research agreement between Washington University and the Monsanto Company. The scientific focus of this venture will be on proteins and peptides which modify cellular behavior. (SK)

  15. Prolonged Correction of Serum Phosphorus in Adults With X-Linked Hypophosphatemia Using Monthly Doses of KRN23.

    PubMed

    Imel, Erik A; Zhang, Xiaoping; Ruppe, Mary D; Weber, Thomas J; Klausner, Mark A; Ito, Takahiro; Vergeire, Maria; Humphrey, Jeffrey S; Glorieux, Francis H; Portale, Anthony A; Insogna, Karl; Peacock, Munro; Carpenter, Thomas O

    2015-07-01

    In X-linked hypophosphatemia (XLH), elevated fibroblast growth factor 23 (FGF23) decreases the renal tubular maximum reabsorption rate of phosphate/glomerular filtration rate (TmP/GFR) and serum inorganic phosphorus (Pi), resulting in rickets and/or osteomalacia. The objective was to test the hypothesis that monthly KRN23 (anti-FGF23 antibody) would safely improve serum Pi in adults with XLH. Two sequential open-label phase 1/2 studies were done. Six academic medical centers were used. Twenty-eight adults with XLH participated in a 4-month dose-escalation study (0.05-0.6 mg/kg); 22 entered a 12-month extension study (0.1-1 mg/kg). KRN23 was injected sc every 28 days. The main outcome measure was the proportion of subjects attaining normal serum Pi and safety. At baseline, mean TmP/GFR, serum Pi, and 1,25-dihydroxyvitamin D [1,25(OH)2D] were 1.6 ± 0.4 mg/dL, 1.9 ± 0.3 mg/dL, and 36.6 ± 14.3 pg/mL, respectively. During dose escalation, TmP/GFR, Pi, and 1,25(OH)2D increased, peaking at 7 days for TmP/GFR and Pi and at 3-7 days for 1,25(OH)2D, remaining above (TmP/GFR, Pi) or near [1,25(OH)2D] pre-dose levels at trough. After each of the four escalating doses, peak Pi was between 2.5 and 4.5 mg/dL in 14.8, 37.0, 74.1, and 88.5% of subjects, respectively. During the 12-month extension, peak Pi was in the normal range for 57.9-85.0% of subjects, and ≥25% maintained trough Pi levels within the normal range. Serum Pi did not exceed 4.5 mg/dL in any subject. Although 1,25(OH)2D levels increased transiently, mean serum and urinary calcium remained normal. KRN23 treatment increased biomarkers of skeletal turnover and had a favorable safety profile. Monthly KRN23 significantly increased serum Pi, TmP/GFR, and 1,25(OH)2D in all subjects. KRN23 has potential for effectively treating XLH.

  16. Structural, Dielectric, and Electrical Properties of Bi1- x Pb x Fe1- x (Zr0.5Ti0.5) x O3

    NASA Astrophysics Data System (ADS)

    Panda, Niranjan; Pattanayak, Samita; Choudhary, R. N. P.

    2015-12-01

    Polycrystalline samples of Bi1- x Pb x Fe1- x (Zr0.5Ti0.5) x O3 (BPFZTO) with x = 0.0, 0.2, 0.3, and 0.4 were prepared by high-temperature solid-state reaction. Preliminary structural analysis of calcined powders of the materials by use of x-ray powder diffraction confirmed formation of single-phase systems with the tetragonal structure. Room-temperature scanning electron micrographs of the samples revealed uniform distribution of grains of low porosity and different dimensions on the surface of the samples. The frequency-temperature dependence of dielectric and electric properties was studied by use of dielectric and complex impedance spectroscopy over a wide range of frequency (1 kHz to 1 MHz) at different temperatures (25-500°C). The dielectric constant of BiFeO3 (BFO) was enhanced by substitution with Pb(Zr0.5Ti0.5)O3 (PZT) whereas the dielectric loss of the BPFZTO compounds decreased with increasing PZT content. A significant contribution of both grains and grain boundaries to the electrical response of the materials was observed. The frequency-dependence of the ac conductivity of BPFZTO followed Jonscher's power law. Negative temperature coefficient of resistance behavior was observed for all the BPFZTO samples. Conductivity by thermally excited charge carriers and oxygen vacancies in the materials was believed to be of the Arrhenius-type.

  17. No detection of SV40 DNA in mesothelioma tissues from a high incidence area in Sweden.

    PubMed

    Lundstig, Annika; Dejmek, Annika; Eklund, Carina; Filinic, Ivo; Dillner, Joakim

    2007-01-01

    Simian virus 40 (SV40), a polyoma virus of the rhesus macaque was discovered in 1960 as a contaminant of human polio vaccines produced in monkey cells. A number of studies have reported the detection of SV40 nucleotide sequences in human tumors, mainly mesotheliomas, but the reports have not been consistent. The presence of SV40 in 26 consecutive cases of malignant mesothelioma of biphasic type was investigated using a SV40 quantitative real time polymerase chain reaction (PCR) with a sensitivity of 10 copies of viral DNA per sample. All the samples were also tested for amplifiability using a real-time PCR for the beta-globin gene. Eighteen tumors were amplifiable, but none contained SV40 DNA. The results do not support an association between mesothelioma and SV40.

  18. (Ba1-x Bi x )(Ti1-x Ni0.5x Sn0.5x )O3 Solid Solution: Phase Evolution, Microstructure, Dielectric Properties, and Impedance Analysis

    NASA Astrophysics Data System (ADS)

    Chen, Xiuli; Li, Xiaoxia; Yan, Xiao; Liu, Gaofeng; Zhou, Huanfu

    2018-02-01

    (Ba1-x Bi x )(Ti1-x Ni0.5x Sn0.5x )O3 (BBTNS, 0.02 ≤ x ≤ 0.1) samples have been synthesized by traditional solid-state reaction technique and their structural transformation and dielectric properties investigated. X-ray diffraction (XRD) analysis revealed that BBTNS could form a homogeneous solid solution, and the transformation from tetragonal to pseudocubic phase occurred at 0.04 ≤ &!nbsp;x ≤ 0.06. Optimized properties with stable ɛ r (˜ 1829 to 1838), small Δɛ/ɛ 25°C values (± 15%) over a broad temperature range from -60°C to 140°C, and low tan Δ (≤ 0.02) from 4°C to 194°C were obtained at x = 0.1. The relaxation and conduction process in the high-temperature region are attributed to thermal activation, and oxygen vacancies may be the ionic charge carriers in perovskite ferroelectrics.

  19. Synaptic vesicle glycoprotein 2C (SV2C) modulates dopamine release and is disrupted in Parkinson disease.

    PubMed

    Dunn, Amy R; Stout, Kristen A; Ozawa, Minagi; Lohr, Kelly M; Hoffman, Carlie A; Bernstein, Alison I; Li, Yingjie; Wang, Minzheng; Sgobio, Carmelo; Sastry, Namratha; Cai, Huaibin; Caudle, W Michael; Miller, Gary W

    2017-03-14

    Members of the synaptic vesicle glycoprotein 2 (SV2) family of proteins are involved in synaptic function throughout the brain. The ubiquitously expressed SV2A has been widely implicated in epilepsy, although SV2C with its restricted basal ganglia distribution is poorly characterized. SV2C is emerging as a potentially relevant protein in Parkinson disease (PD), because it is a genetic modifier of sensitivity to l-DOPA and of nicotine neuroprotection in PD. Here we identify SV2C as a mediator of dopamine homeostasis and report that disrupted expression of SV2C within the basal ganglia is a pathological feature of PD. Genetic deletion of SV2C leads to reduced dopamine release in the dorsal striatum as measured by fast-scan cyclic voltammetry, reduced striatal dopamine content, disrupted α-synuclein expression, deficits in motor function, and alterations in neurochemical effects of nicotine. Furthermore, SV2C expression is dramatically altered in postmortem brain tissue from PD cases but not in Alzheimer disease, progressive supranuclear palsy, or multiple system atrophy. This disruption was paralleled in mice overexpressing mutated α-synuclein. These data establish SV2C as a mediator of dopamine neuron function and suggest that SV2C disruption is a unique feature of PD that likely contributes to dopaminergic dysfunction.

  20. 50 CFR 15.23 - Permits for zoological breeding or display programs.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... OF WILDLIFE AND PLANTS WILD BIRD CONSERVATION ACT Permits and Approval of Cooperative Breeding Programs § 15.23 Permits for zoological breeding or display programs. (a) Application requirements for... 50 Wildlife and Fisheries 1 2011-10-01 2011-10-01 false Permits for zoological breeding or display...

  1. 50 CFR 15.23 - Permits for zoological breeding or display programs.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... OF WILDLIFE AND PLANTS WILD BIRD CONSERVATION ACT Permits and Approval of Cooperative Breeding Programs § 15.23 Permits for zoological breeding or display programs. (a) Application requirements for... 50 Wildlife and Fisheries 1 2010-10-01 2010-10-01 false Permits for zoological breeding or display...

  2. A Functional Role for KLF6-SV1 in Lung Adenocarcinoma Prognosis and Chemotherapy Response

    PubMed Central

    DiFeo, Analisa; Feld, Lauren; Rodriguez, Estefania; Wang, Christine; Beer, David G.; Martignetti, John A.; Narla, Goutham

    2009-01-01

    Kruppel-like factor 6 (KLF6) is a tumor suppressor gene that is functionally inactivated in human cancer by loss of heterozygosity, somatic mutation, decreased expression, and increased alternative splicing into an oncogenic splice variant, KLF6-SV1. Here we show that increased expression of KLF6-SV1 is associated with decreased survival in patients with lung adenocarcinoma. In addition, KLF6-SV1 is a novel antiapoptotic protein in lung cancer cell lines, and targeted reduction of KLF6-SV1 using siRNA induces apoptosis both alone and in combination with the chemotherapeutic drug cisplatin. Together, these findings highlight a critical role for KLF6-SV1 in lung cancer, and show a potential novel therapeutic strategy for the treatment of lung cancer. PMID:18250346

  3. Soft ferromagnetism in mixed valence Sr(1-x)La(x)Ti(0.5)Mn(0.5)O₃ perovskites.

    PubMed

    Qasim, Ilyas; Blanchard, Peter E R; Kennedy, Brendan J; Ling, Chris D; Jang, Ling-Yun; Kamiyama, Takashi; Miao, Ping; Torii, Shuki

    2014-05-14

    The structural, magnetic and electrical properties of the mixed Ti-Mn oxides Sr(1-x)La(x)Ti(0.5)Mn(0.5)O3 (0 ≤ x ≤ 0.5) are reported. At room temperature the oxides have a cubic structure in space group Pm3m for x ≤ 0.25 and rhombohedral in R3c for 0.3 ≤ x ≤ 0.50. X-ray absorption spectroscopic measurements demonstrate the addition of La(3+) is compensated by the partial reduction of Mn(4+) to Mn(3+). Variable temperature neutron diffraction measurements show that cooling Sr(0.6)La(0.4)Ti(0.5)Mn(0.5)O3 results in a first order transition from rhombohedra to an orthorhombic structure in Imma. Complex magnetic behaviour is observed. The magnetic behaviour of the mixed valent (Mn(3+/4+)) examples is dominated by ferromagnetic interactions, although cation disorder frustrates long range magnetic ordering.

  4. 50 CFR 23.79 - How may I participate in the Plant Rescue Center Program?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false How may I participate in the Plant Rescue Center Program? 23.79 Section 23.79 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... may I participate in the Plant Rescue Center Program? (a) Purpose. We have established the Plant...

  5. 23 CFR 1200.23 - Impaired driving countermeasures grants.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Impaired driving countermeasures grants. 1200.23 Section... HIGHWAY SAFETY GRANT PROGRAMS National Priority Safety Program Grants § 1200.23 Impaired driving countermeasures grants. (a) Purpose. This section establishes criteria, in accordance with 23 U.S.C. 405(d), for...

  6. 23 CFR 1200.23 - Impaired driving countermeasures grants.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Impaired driving countermeasures grants. 1200.23 Section... HIGHWAY SAFETY GRANT PROGRAMS National Priority Safety Program Grants § 1200.23 Impaired driving countermeasures grants. (a) Purpose. This section establishes criteria, in accordance with 23 U.S.C. 405(d), for...

  7. Synthesis and characterization of (1-x)Bi(Mg{sub 2/3}Sb{sub 1/3})O{sub 3}-xPbTiO{sub 3} piezoceramics

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Upadhyay, Ashutosh; Dwivedi, Saurabh; Pandey, Rishikesh

    2016-05-23

    We present here the comprehensive x-ray diffraction and polarization-electric field hysteresis studies on (1-x)Bi(Mg{sub 2/3}Sb{sub 1/3})O{sub 3}-xPbTiO{sub 3} piezoceramics with x = 0.52, 0.56 and 0.60. The powder x-ray diffraction data reveals the presence of tetragonal phase for all the compositions. The saturation of hysteresis loop is observed for x ≤ 0.56.

  8. The geometry of the close environment of SV Piscium as probed by VLTI/MIDI

    NASA Astrophysics Data System (ADS)

    Klotz, D.; Sacuto, S.; Kerschbaum, F.; Paladini, C.; Olofsson, H.; Hron, J.

    2012-05-01

    Context. SV Psc is an asymptotic giant branch (AGB) star surrounded by an oxygen-rich dust envelope. The mm-CO line profile of the object's outflow shows a clear double-component structure. Because of the high angular resolution, mid-IR interferometry may give strong constraints on the origin of this composite profile. Aims: The aim of this work is to investigate the morphology of the environment around SV Psc using high-angular resolution interferometry observations in the mid-IR with the Very Large Telescope MID-infrared Interferometric instrument (VLTI/MIDI). Methods: Interferometric data in the N-band taken at different baseline lengths (ranging from 32-64 m) and position angles (73-142°) allow a study of the morphology of the circumstellar environment close to the star. The data are interpreted on the basis of 2-dimensional, chromatic geometrical models using the fitting software tool GEM-FIND developed for this purpose. Results: The results favor two scenarios: (i) the presence of a highly inclined, optically thin, dusty disk surrounding the central star; (ii) the presence of an unresolved binary companion at a separation of 13.7+4.2-4.8 AU and a position angle of 121.8°+15.4°-24.5° NE. The derived orbital period of the binary is 38.1+20.4-22.6 yr. This detection is in good agreement with hydrodynamic simulations showing that a close companion could be responsible for the entrainment of the gas and dust into a circumbinary structure. Based on observations made with ESO telescopes at La Silla Paranal Observatory under program IDs 082.D-0389 and 086.D-0069.

  9. Thermoelectric and Magnetic Properties of Sn1- x O2:Mn0.5 x Co0.5 x Nanoparticles Produced by the Microwave Technique

    NASA Astrophysics Data System (ADS)

    Salah, Numan; Habib, Sami; Azam, Ameer

    2017-02-01

    Nanoparticles (NPs) of Sn1- x O2:Mn0.5 x Co0.5 x with x = 0.02, 0.04, 0.06, 0.08 and 0.1 were synthesized by the microwave-assisted route and characterized for their thermoelectric and magnetic properties. As a result of Mn and Co co-doping, a considerable increase in the values of energy band gap and lattice constant c of Sn1- x O2:Mn0.5 x Co0.5 x NPs was observed. The x-ray photoelectron spectroscopy spectra revealed that Mn and Co ions were incorporated in their 4+ and 2+ states, respectively. The resistivity and calculated activation energy of these NPs were found to decrease by increasing the Mn and Co contents. A negative Seebeck coefficient was observed, whose value was found to be significantly increased by increasing the value of x. The magnetic measurement results revealed that all the microwave-synthesized Sn1- x O2:Mn0.5 x Co0.5 x NPs including the pure SnO2 have distinctly wide hysteresis loops. This indicates that samples have room-temperature ferromagnetism. The optimum value for x to have maximum saturation magnetism was observed to be 0.04. Diamagnetic contributions from the core of these NPs were noticed at higher magnetic fields. The observed magnetism was attributed to the presence of defects at the NPs' interfacing sites, grain boundaries, atom vacancies and an optimum level of Mn and Co co-dopants. The observed wide hysteresis loops in these NPs might be useful for producing nanoscale magnets and magnetic memory devices. Moreover, the observed thermoelectric properties, i.e. Seebeck coefficient and power factor in these NPs, might be useful for the development of thermoelectric devices.

  10. Interpreting the structural and electrochemical complexity of 0.5Li{sub 2}MnO{sub 3}{lg_bullet}.0.5LiMO{sub 2} electrodes for lithium batteries (M=Mn{sub 0.5-x}Ni{sub 0.5-x}Co{sub 2x}, 0{le}x{le}0.5).

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kang, S. H.; Kempgens, P.; Greenbaum, S.

    2007-01-01

    The structural and electrochemical features of layered 0.5Li{sub 2}MnO{sub 3} {center_dot} 0.5LiMO{sub 2} electrodes, in which M = Mn{sub 0.5-x}Ni{sub 0.5-x}Co{sub 2x} (0{le} x {le} 0.5), have been studied by powder X-ray diffraction, electrochemical differential-capacity measurements, {sup 7}Li magic-angle-spinning nuclear magnetic resonance, and X-ray absorption near-edge spectroscopy. Li{sub 2}MnO{sub 3}-like regions in the as-prepared samples were observed for all values of x, with transition-metal cation disorder between the LiMO{sub 2} and Li{sub 2}MnO{sub 3} components increasing with cobalt content (i.e., the value of x). The structural disorder and complexity of the electrochemical redox reactions increase when the Li{sub 2}MnO{sub 3}-likemore » regions within the electrode are activated to 4.6 V in lithium cells; interpretations of structural and electrochemical phenomena are provided.« less

  11. Prolonged Correction of Serum Phosphorus in Adults With X-Linked Hypophosphatemia Using Monthly Doses of KRN23

    PubMed Central

    Zhang, Xiaoping; Ruppe, Mary D.; Weber, Thomas J.; Klausner, Mark A.; Ito, Takahiro; Vergeire, Maria; Humphrey, Jeffrey S.; Glorieux, Francis H.; Portale, Anthony A.; Insogna, Karl; Peacock, Munro; Carpenter, Thomas O.

    2015-01-01

    Context: In X-linked hypophosphatemia (XLH), elevated fibroblast growth factor 23 (FGF23) decreases the renal tubular maximum reabsorption rate of phosphate/glomerular filtration rate (TmP/GFR) and serum inorganic phosphorus (Pi), resulting in rickets and/or osteomalacia. Objective: The objective was to test the hypothesis that monthly KRN23 (anti-FGF23 antibody) would safely improve serum Pi in adults with XLH. Design: Two sequential open-label phase 1/2 studies were done. Setting: Six academic medical centers were used. Participants: Twenty-eight adults with XLH participated in a 4-month dose-escalation study (0.05–0.6 mg/kg); 22 entered a 12-month extension study (0.1–1 mg/kg). Intervention: KRN23 was injected sc every 28 days. Main Outcome Measure: The main outcome measure was the proportion of subjects attaining normal serum Pi and safety. Results: At baseline, mean TmP/GFR, serum Pi, and 1,25-dihydroxyvitamin D [1,25(OH)2D] were 1.6 ± 0.4 mg/dL, 1.9 ± 0.3 mg/dL, and 36.6 ± 14.3 pg/mL, respectively. During dose escalation, TmP/GFR, Pi, and 1,25(OH)2D increased, peaking at 7 days for TmP/GFR and Pi and at 3–7 days for 1,25(OH)2D, remaining above (TmP/GFR, Pi) or near [1,25(OH)2D] pre-dose levels at trough. After each of the four escalating doses, peak Pi was between 2.5 and 4.5 mg/dL in 14.8, 37.0, 74.1, and 88.5% of subjects, respectively. During the 12-month extension, peak Pi was in the normal range for 57.9–85.0% of subjects, and ≥25% maintained trough Pi levels within the normal range. Serum Pi did not exceed 4.5 mg/dL in any subject. Although 1,25(OH)2D levels increased transiently, mean serum and urinary calcium remained normal. KRN23 treatment increased biomarkers of skeletal turnover and had a favorable safety profile. Conclusions: Monthly KRN23 significantly increased serum Pi, TmP/GFR, and 1,25(OH)2D in all subjects. KRN23 has potential for effectively treating XLH. PMID:25919461

  12. Visualization of SV2A conformations in situ by the use of Protein Tomography

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lynch, Berkley A.; Matagne, Alain; Braennstroem, Annika

    2008-10-31

    The synaptic vesicle protein 2A (SV2A), the brain-binding site of the anti-epileptic drug levetiracetam (LEV), has been characterized by Protein Tomography{sup TM}. We identified two major conformations of SV2A in mouse brain tissue: first, a compact, funnel-structure with a pore-like opening towards the cytoplasm; second, a more open, V-shaped structure with a cleft-like opening towards the intravesicular space. The large differences between these conformations suggest a high degree of flexibility and support a valve-like mechanism consistent with the postulated transporter role of SV2A. These two conformations are represented both in samples treated with LEV, and in saline-treated samples, which indicatesmore » that LEV binding does not cause a large-scale conformational change of SV2A, or lock a specific conformational state of the protein. This study provides the first direct structural data on SV2A, and supports a transporter function suggested by sequence homology to MFS class of transporter proteins.« less

  13. Stereostructure of a norbornane diexo-condensed 1,3-oxazine[2,3-a]isoindolone An X-ray study

    NASA Astrophysics Data System (ADS)

    Argay, Gy.; Kálmán, A.; Stájer, G.; Bernáth, G.

    1994-12-01

    The structure of 3,6-methano-12b-p-tolyl-2a,3,4,5,6,6a,7,12b-octahydro-2H-3,1-benzoxazino[2,3-a]isoindol-8-one (C23H23NO2, Mr = 345.42), prepared from 2-p-toluoylbenzoic acid and diexo-3-hydroxymethylbicyclo[2.2.1]heptyl-2-amine, has been established by X-ray crystallography from diffractometer data: it crystallizes in the triclinic space group Poverline1 with a = 8.186(2) Å, b = 8.683(2) Å, c = 13.324(5) Å, α = 83.46(4)°, β = 75.48(6)°, γ = 77.60(4)°, V = 893.6(4) Å3, Z = 2, Dc = 1.284 g cm-3 and μ(Cu Kα) = 0.641 mm-1. The structure was solved by direct methods and refined to R = 0.061 for 3685 observed reflections. The molecule exhibits diexo-annelation between the 1,3-oxazine ring and the norbornane moiety. The p-tolyl group and norbonane annelation hydrogens are trans and the 1,3-oxazine ring is fused to an almost planar γ-lactam ring. Consequently, the six-membered hetero ring assumes an irregular conformation among the canonical forms 1S6, 1T3 and B3,6.

  14. Low NO/x/ heavy fuel combustor program

    NASA Technical Reports Server (NTRS)

    Lister, E.; Niedzwiecki, R. W.; Nichols, L.

    1980-01-01

    The paper deals with the 'Low NO/x/ Heavy Fuel Combustor Program'. Main program objectives are to generate and demonstrate the technology required to develop durable gas turbine combustors for utility and industrial applications, which are capable of sustained, environmentally acceptable operation with minimally processed petroleum residual fuels. The program will focus on 'dry' reductions of oxides of nitrogen (NO/x/), improved combustor durability and satisfactory combustion of minimally processed petroleum residual fuels. Other technology advancements sought include: fuel flexibility for operation with petroleum distillates, blends of petroleum distillates and residual fuels, and synfuels (fuel oils derived from coal or shale); acceptable exhaust emissions of carbon monoxide, unburned hydrocarbons, sulfur oxides and smoke; and retrofit capability to existing engines.

  15. An oxidant- and solvent-stable protease produced by Bacillus cereus SV1: application in the deproteinization of shrimp wastes and as a laundry detergent additive.

    PubMed

    Manni, Laila; Jellouli, Kemel; Ghorbel-Bellaaj, Olfa; Agrebi, Rym; Haddar, Anissa; Sellami-Kamoun, Alya; Nasri, Moncef

    2010-04-01

    The current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. In this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by Bacillus cereus SV1. Maximum protease activity (5,900 U/mL) was obtained when the strain was grown in medium containing 40 g/L shrimp wastes powder as a sole carbon source. The optimum pH, optimum temperature, pH stability, and thermal stability of the crude enzyme preparation were pH 8.0, 60 degrees C, pH 6-9.5, and <55 degrees C, respectively. The crude protease was extremely stable toward several organic solvents. No loss of activity was observed even after 60 days of incubation at 30 degrees C in the presence of 50% (v/v) dimethyl sulfoxide and ethyl ether; the enzyme retained more than 70% of its original activity in the presence of ethanol and N,N-dimethylformamide. The protease showed high stability toward anionic (SDS) and non-ionic (Tween 80, Tween 20, and Triton X-100) surfactants. Interestingly, the activity of the enzyme was significantly enhanced by oxidizing agents. In addition, the enzyme showed excellent compatibility with some commercial liquid detergents. The protease of B. cereus SV1, produced under the optimal culture conditions, was tested for shrimp waste deproteinization in the preparation of chitin. The protein removal with a ratio E/S of 20 was about 88%. The novelties of the SV1 protease include its high stability to organic solvents and surfactants. These unique properties make it an ideal choice for application in detergent formulations and enzymatic peptide synthesis. In addition, the enzyme may find potential applications in the deproteinization of shrimp wastes to produce chitin.

  16. Potassium vanadate K0.23V2O5 as anode materials for lithium-ion and potassium-ion batteries

    NASA Astrophysics Data System (ADS)

    Liu, Cailing; Luo, Shaohua; Huang, Hongbo; Wang, Zhiyuan; Wang, Qing; Zhang, Yahui; Liu, Yanguo; Zhai, Yuchun; Wang, Zhaowen

    2018-06-01

    A layered potassium vanadate K0.23V2O5 has been successfully prepared by the hydrothermal method and evaluated as an anode material for lithium-ion and potassium-ion batteries. High structural stability is demonstrated by the ex situ X-ray diffraction (XRD) and ex situ scanning electron microscopy (SEM). When used as an anode material for lithium-ion batteries, the K0.23V2O5 exhibits a reversible capacity of 480.4 mAh g-1 at 20 mA g-1 after 100 cycles and 439.7 mAh g-1 at 200 mA g-1 after 300 cycles as well as good cycling stability. Even at a high current density of 800 mA g-1, a high reversible capacity of 202.5 mAh g-1 can be retained, indicating excellent rate performance. Whereas in potassium-ion batteries, it retains a capacity of 121.6 mAh g-1 after 150 cycles at 20 mA g-1 and 97.6 mAh g-1 at 100 mA g-1 after 100 cycles. Such superior electrochemical performance of K0.23V2O5 can be ascribed to the special flower-like morphology and structure. Overall, the results highlight the great potential of K0.23V2O5 as an anode material for both lithium-ion and potassium-ion batteries.

  17. Photographic copy of 3 ½” x 5” glass lantern slide ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photographic copy of 3 ½” x 5” glass lantern slide no. 5A of June 1926 and November 1930 drawing by Ralph Modjeski, Consulting Engineer. Located in wooden pine box #23 in box 84 of 94 at the National Museum of American History, Smithsonian Institution, Archives Center, Work and Industry Division, Washington, D.C. Original photographer unknown. JUNE 1926 AND NOVEMBER 1930 DRAWING OF “PROPOSED BRIDGE OVER THE MISSISSIPPI RIVER NORTH OF NEW ORLEANS, LOUISIANA, PLAN “E”. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  18. Systemic Absorption of Rifamycin SV MMX Administered as Modified-Release Tablets in Healthy Volunteers▿

    PubMed Central

    Di Stefano, A. F. D.; Rusca, A.; Loprete, L.; Dröge, M. J.; Moro, L.; Assandri, A.

    2011-01-01

    The new oral 200-mg rifamycin SV MMX modified-release tablets, designed to deliver rifamycin SV directly into the colonic lumen, offer considerable advantages over the existing immediate-release antidiarrheic formulations. In two pharmacokinetics studies of healthy volunteers, the absorption, urinary excretion, and fecal elimination of rifamycin SV after single- and multiple-dose regimens of the new formulation were investigated. Concentrations in plasma of >2 ng/ml were infrequently and randomly quantifiable after single and multiple oral doses. The systemic exposure to rifamycin SV after single and multiple oral doses of MMX tablets under fasting and fed conditions or following a four-times-a-day (q.i.d.) or a twice-a-day (b.i.d.) regimen could be considered negligible. With both oral regimens, the drug was confirmed to be very poorly absorbable systemically. The amount of systemically absorbed antibiotic excreted by the renal route is far lower than 0.01% of the administered dose after both the single- and multiple-dose regimens. The absolute bioavailability, calculated as the mean percent ratio between total urinary excretion amounts (ΣXu) after a single intravenous injection and after a single oral dose under fasting conditions, was 0.0410 ± 0.0617. The total elimination of the unchanged rifamycin SV with feces was 87% of the administered oral dose. No significant effect of rifamycin SV on vital signs, electrocardiograms, or laboratory parameters was observed. PMID:21402860

  19. Technology Transfer Program (TTP). Quality Assurance System. Volume 2. Appendices

    DTIC Science & Technology

    1980-03-03

    LSCo Report No. - 2X23-5.1-4-I TECHNOLOGY TRANSFER PROGRAM (TTP) FINAL REPORT QUALITY ASSURANCE SYSTEM Appendix A Accuracy Control System QUALITY...4-1 TECHNOLOGY TRANSFER PROGRAM (TTP) FINAL REPORT QUALITY ASSURANCE SYSTEM Appendix A Accuracy Control System QUALITY ASSURANCE VOLUME 2 APPENDICES...prepared by: Livingston Shipbuilding Company Orange, Texas March 3, 1980 APPENDIX A ACCURACY CONTROL SYSTEM . IIII MARINE TECHNOLOGY. INC. HP-121

  20. Identification, expression, and pharmacology of a Cys{sub 23}-Ser{sub 23} substitution in the human 5-HT{sub 2C} receptor gene (HTR2C)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lappalainen, J.; Ozaki, N.; Goldman, D.

    1995-05-20

    The function of brain serotonin-2C (5-HT{sub 2C}) receptors, including behavioral and neurochemical responses to 5-HT{sub 2C} agonist challenge, has been suggested to be abnormal in individuals with neuropsychiatric disorders. Thus, it is important to identify polymorphisms and functional variants within this gene. Using SSCP analysis, the authors identified a Cys{sub 23}-Ser{sub 23} substitution (designated 5-HT{sub 2Ccys} and 5-HT{sub 2Cser}) in the first hydrophobic region of the human 5-HT{sub 2C} receptor. Allele frequencies in unrelated Caucasians were 0.13 and 0.87 for 5-HT{sub 2Cser} and 5-HT{sub 2Ccys}, respectively. DNAs from informative CEPH families were typed for this polymorphism and analyzed with respectmore » to 20 linked markers on the X chromosome. Linkage analysis placed the 5-HT{sub 2C} receptor gene (HTR2C) on Xq24. To evaluate whether this amino acid substitution causes a variant function of this receptor, recombinant human 5-HT{sub 2Ccys} and 5-HT{sub 2Cser} receptors were expressed in Xenopus oocytes and tested for responses to 5-HT using electrophysiological techniques. Concentration-response curves for 5-HT were not significantly different in oocytes expressing either form of the receptor, suggesting that the 5-HT{sub 2Ccys} and 5-HT{sub 2Cser} receptor proteins may not differ in their responses to serotonin under baseline physiological conditions. 43 refs., 3 figs., 1 tab.« less

  1. SACD's Support of the Hyper-X Program

    NASA Technical Reports Server (NTRS)

    Robinson, Jeffrey S.; Martin, John G.

    2006-01-01

    NASA s highly successful Hyper-X program demonstrated numerous hypersonic air-breathing vehicle related technologies including scramjet performance, advanced materials and hot structures, GN&C, and integrated vehicle performance resulting in, for the first time ever, acceleration of a vehicle powered by a scramjet engine. The Systems Analysis and Concepts Directorate (SACD) at NASA s Langley Research Center played a major role in the integrated team providing critical support, analysis, and leadership to the Hyper-X Program throughout the program s entire life and were key to its ultimate success. Engineers in SACD s Vehicle Analysis Branch (VAB) were involved in all stages and aspects of the program, from conceptual design prior to contract award, through preliminary design and hardware development, and in to, during, and after each of the three flights. Working closely with other engineers at Langley and Dryden, as well as industry partners, roughly 20 members of SACD were involved throughout the evolution of the Hyper-X program in nearly all disciplines, including lead roles in several areas. Engineers from VAB led the aerodynamic database development, the propulsion database development, and the stage separation analysis and database development effort. Others played major roles in structures, aerothermal, GN&C, trajectory analysis and flight simulation, as well as providing CFD support for aerodynamic, propulsion, and aerothermal analysis.

  2. Photographic copy of 3 ½” x 5” glass lantern slide ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photographic copy of 3 ½” x 5” glass lantern slide no. 20 of drawing. Located in wooden pine box #23 in box 84 of 94 at the National Museum of American History, Smithsonian Institution, Archives Center, Work and industry Division, Washington, D.C. Original photographer, Milton R. Homes, Philadelphia, Pennsylvania. Illustrator Unknown. DRAWING ENTITLED “THE SAND ISLAND METHOD”. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  3. A multicomponent matched filter cluster confirmation tool for eROSITA: initial application to the RASS and DES-SV data sets

    NASA Astrophysics Data System (ADS)

    Klein, M.; Mohr, J. J.; Desai, S.; Israel, H.; Allam, S.; Benoit-Lévy, A.; Brooks, D.; Buckley-Geer, E.; Carnero Rosell, A.; Carrasco Kind, M.; Cunha, C. E.; da Costa, L. N.; Dietrich, J. P.; Eifler, T. F.; Evrard, A. E.; Frieman, J.; Gruen, D.; Gruendl, R. A.; Gutierrez, G.; Honscheid, K.; James, D. J.; Kuehn, K.; Lima, M.; Maia, M. A. G.; March, M.; Melchior, P.; Menanteau, F.; Miquel, R.; Plazas, A. A.; Reil, K.; Romer, A. K.; Sanchez, E.; Santiago, B.; Scarpine, V.; Schubnell, M.; Sevilla-Noarbe, I.; Smith, M.; Soares-Santos, M.; Sobreira, F.; Suchyta, E.; Swanson, M. E. C.; Tarle, G.; Collaboration, the DES

    2018-03-01

    We describe a multicomponent matched filter (MCMF) cluster confirmation tool designed for the study of large X-ray source catalogues produced by the upcoming X-ray all-sky survey mission eROSITA. We apply the method to confirm a sample of 88 clusters with redshifts 0.05 < z < 0.8 in the recently published 2RXS catalogue from the ROSAT All-Sky Survey (RASS) over the 208 deg2 region overlapped by the Dark Energy Survey (DES) Science Verification (DES-SV) data set. In our pilot study, we examine all X-ray sources, regardless of their extent. Our method employs a multicolour red sequence (RS) algorithm that incorporates the X-ray count rate and peak position in determining the region of interest for follow-up and extracts the positionally and colour-weighted optical richness λMCMF as a function of redshift for each source. Peaks in the λMCMF-redshift distribution are identified and used to extract photometric redshifts, richness and uncertainties. The significances of all optical counterparts are characterized using the distribution of richnesses defined along random lines of sight. These significances are used to extract cluster catalogues and to estimate the contamination by random superpositions of unassociated optical systems. The delivered photometric redshift accuracy is δz/(1 + z) = 0.010. We find a well-defined X-ray luminosity-λMCMF relation with an intrinsic scatter of δln (λMCMF|Lx) = 0.21. Matching our catalogue with the DES-SV redMaPPer catalogue yields good agreement in redshift and richness estimates; comparing our catalogue with the South Pole Telescope (SPT) selected clusters shows no inconsistencies. SPT clusters in our data set are consistent with the high-mass extension of the RASS-based λMCMF-mass relation.

  4. Regulation of X-Ray Security Scanners in Michigan.

    PubMed

    Parry, Donald E

    2016-02-01

    In January of 2013 the Transportation Security Administration (TSA) ordered the removal of x-ray security scanners from airports by June of 2013. Since that time several of these scanners have been purchased at a reduced cost by various state and county governments for use in screening individuals entering or leaving their facilities. To address this issue the Radiation Safety Section of the State of Michigan drafted a set of registration conditions for facilities to follow when using these security scanners. Inspection procedures and measurement protocols were developed to estimate the dose to screened individuals. Inspections were performed on nine of the 16 registered backscatter scanners in the state and the one transmission scanner. The average estimated effective dose to screened individuals was ∼11 nSv for a two view scan from a backscatter system. The effective dose was 0.446 μSv, 0.330 μSv, and 0.150 μSv for a transmission system operated in the high, medium, and low dose modes, respectively. The limit suggested in the new registration condition is 0.25 μSv for a general use system and 10 μSv for a limited use system.

  5. Cross section measurements of radiative KL2,3 RRS in 24Cr and L3M4,5 RRS in 59Pr for Mn Kα1,2 X-rays

    NASA Astrophysics Data System (ADS)

    Sharma, Veena; Upmanyu, Arun; Singh, Ranjit; Singh, Gurjot; Sharma, Hitesh; Kumar, Sanjeev; Mehta, D.

    2017-06-01

    The KL2,3 and L3M4,5 radiative resonant Raman scattering (RRS) cross sections have been measured for the quasimonochromatic Mn Kα1,2 X-rays (5.895 keV) in 24Cr (K-shell level width (ΓK) =1.08 eV) and 59 Pr (L3-subshell level width (ΓL3) =3.60 eV), respectively, using targets in metallic and various chemical forms. The incident Mn Kα1,2 X-ray energy is lower than the K-shell binding energy of 24Cr and L3-subshell binding energy of 59Pr by 94 ΓK (Cr) and 94 ΓL3 (Pr), respectively. The experimental measurements were performed with a low energy Ge detector (LEGe) and a radioactive 55Fe annular source in conjunction with 24Cr absorber. The measured cross section values for the 24Cr and 59 Pr elements in their various oxidation states are found to be same within experimental errors. The measurements were further extended to investigate alignment of the intermediate L3-subshell (J =3/2) virtual vacancy states in 59Pr through angular distribution measurements for RRS photon emission, which is found to be isotropic within experimental errors.

  6. SV40-transformed human fibroblasts: evidence for cellular aging in pre-crisis cells.

    PubMed

    Stein, G H

    1985-10-01

    Pre-crisis SV40-transformed human diploid fibroblast (HDF) cultures have a finite proliferative lifespan, but they do not enter a viable senescent state at end of lifespan. Little is known about either the mechanism for this finite lifespan in SV40-transformed HDF or its relationship to finite lifespan in normal HDF. Recently we proposed that in normal HDF the phenomena of finite lifespan and arrest in a viable senescent state depend on two separate processes: 1) an age-related decrease in the ability of the cells to recognize or respond to serum and/or other mitogens such that the cells become functionally mitogen-deprived at the end of lifespan; and 2) the ability of the cells to enter a viable, G1-arrested state whenever they experience mitogen deprivation. In this paper, data are presented that suggest that pre-crisis SV40-transformed HDF retain the first process described above, but lack the second process. It is shown that SV40-transformed HDF have a progressively decreasing ability to respond to serum as they age, but they continue to traverse the cell cycle at the end of lifespan. Concomitantly, the rate of cell death increases steadily toward the end of lifespan, thereby causing the total population to cease growing and ultimately to decline. Previous studies have shown that when SV40-transformed HDF are environmentally serum deprived, they likewise exhibit continued cell cycle traverse coupled with increased cell death. Thus, these results support the hypothesis that pre-crisis SV40-transformed HDF still undergo the same aging process as do normal HDF, but they end their lifespan in crisis rather than in the normal G1-arrested senescent state because they have lost their ability to enter a viable, G1-arrested state in response to mitogen deprivation.

  7. Design, Synthesis, and X-ray Crystal Structures of 2,4-Diaminofuro[2,3-d]pyrimidines as Multireceptor Tyrosine Kinase and Dihydrofolate Reductase Inhibitors

    PubMed Central

    Gangjee, Aleem; Li, Wei; Lin, Lu; Zeng, Yibin; Ihnat, Michael; Warnke, Linda A.; Green, Dixy W.; Cody, Vivian; Pace, Jim; Queener, Sherry F.

    2009-01-01

    To optimize dual receptor tyrosine kinase (RTK) and dihydrofolate reductase (DHFR) inhibition, the E- and Z-isomers of 5-[2-(2-methoxyphenyl)prop-1-en-1-yl]furo[2,3-d]pyrimidine-2,4-diamines (1a and 1b) were separated by HPLC and the X-ray crystal structures (2.0 Å and 1.4 Å respectively) with mouse DHFR and NADPH as well as 1b with human DHFR (1.5 Å) were determined. The E- and Z-isomers adopt different binding modes when bound to mouse DHFR. A series of 2,4-diaminofuro[2,3-d]pyrimidines 2–13 were designed and synthesized using the X-ray crystal structures of 1a and 1b with DHFR to increase their DHFR inhibitory activity. Wittig reactions of appropriate 2-methoxyphenyl ketones with 2,4-diamino-6-chloromethyl furo[2,3-d]pyrimidine afforded the C8–C9 unsaturated compounds 2–7 and catalytic reduction gave the saturated 8–13. Homologation of the C9-methyl analog maintains DHFR inhibitory activity. In addition, inhibition of EGFR and PDGFR-β were discovered for saturated C9-homologated analogs 9 and 10 that were absent in the saturated C9-methyl analogs. PMID:19748785

  8. 30 CFR 736.23 - Federal program effect on State law or regulations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

  9. 30 CFR 736.23 - Federal program effect on State law or regulations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

  10. 30 CFR 736.23 - Federal program effect on State law or regulations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

  11. 30 CFR 736.23 - Federal program effect on State law or regulations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

  12. 30 CFR 736.23 - Federal program effect on State law or regulations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... FOR A STATE § 736.23 Federal program effect on State law or regulations. (a) Whenever a Federal program is promulgated or revised for a State, any statutes or regulations of the State regulating coal... in the Federal Register any State statute or regulation which is preempted and superseded by the...

  13. The IHS diagnostic X-ray equipment radiation protection program

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Knapp, A.; Byrns, G.; Suleiman, O.

    The Indian Health Service (IHS) operates or contracts with Tribal groups to operate 50 hospitals and approximately 165 primary ambulatory care centers. These facilities contain approximately 275 medical and 800 dental diagnostic x-ray machines. IHS environmental health personnel in collaboration with the Food and Drug Administration's (FDA) Center for Devices and Radiological Health (CDRH) developed a diagnostic x-ray protection program including standard survey procedures and menu-driven calculations software. Important features of the program include the evaluation of equipment performance collection of average patient entrance skin exposure (ESE) measurements for selected procedures, and quality assurance. The ESE data, collected using themore » National Evaluation of X-ray Trends (NEXT) protocol, will be presented. The IHS Diagnostic X-ray Radiation Protection Program is dynamic and is adapting to changes in technology and workload.« less

  14. An x-ray diffraction study of some mesoionic 2,3-diphenyltetrazoles

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Luboradzki, R.; Kozminski, W.; Stefaniak, L.

    1993-02-01

    An X-my diffraction study is reported for four molecules of mesoionic 2,3-diphenyltetrazoles. The results confirm a dipolar [open quotes]mesoionic[close quotes] structure, aromatic character of the tetrazole ring and no conjugation between the phenyl and tetrazole rings. The geometry of the exocyclic group is discussed in detail. The molecular parameters of the compounds investigated are correlated with [sup 13]C and [sup 15]N nmr data. The results obtained are compared with similar structures which have already been studied.

  15. p53 elevation in human cells halt SV40 infection by inhibiting T-ag expression

    PubMed Central

    Drayman, Nir; Ben-nun-Shaul, Orly; Butin-Israeli, Veronika; Srivastava, Rohit; Rubinstein, Ariel M.; Mock, Caroline S.; Elyada, Ela; Ben-Neriah, Yinon; Lahav, Galit; Oppenheim, Ariella

    2016-01-01

    SV40 large T-antigen (T-ag) has been known for decades to inactivate the tumor suppressor p53 by sequestration and additional mechanisms. Our present study revealed that the struggle between p53 and T-ag begins very early in the infection cycle. We found that p53 is activated early after SV40 infection and defends the host against the infection. Using live cell imaging and single cell analyses we found that p53 dynamics are variable among individual cells, with only a subset of cells activating p53 immediately after SV40 infection. This cell-to-cell variabilty had clear consequences on the outcome of the infection. None of the cells with elevated p53 at the beginning of the infection proceeded to express T-ag, suggesting a p53-dependent decision between abortive and productive infection. In addition, we show that artificial elevation of p53 levels prior to the infection reduces infection efficiency, supporting a role for p53 in defending against SV40. We further found that the p53-mediated host defense mechanism against SV40 is not facilitated by apoptosis nor via interferon-stimulated genes. Instead p53 binds to the viral DNA at the T-ag promoter region, prevents its transcriptional activation by Sp1, and halts the progress of the infection. These findings shed new light on the long studied struggle between SV40 T-ag and p53, as developed during virus-host coevolution. Our studies indicate that the fate of SV40 infection is determined as soon as the viral DNA enters the nucleus, before the onset of viral gene expression. PMID:27462916

  16. New adatom model for Si(11) 7X7 and Si(111)Ge 5X5 reconstructed surfaces

    NASA Technical Reports Server (NTRS)

    Chadi, D. J.

    1985-01-01

    A new adatom model differing from the conventional model by a reconstruction of the substrate is proposed. The new adatom structure provides an explanation for the 7x7 and 5x5 size of the unit cells seen on annealed Si(111) and Si(111)-Ge surfaces, respectively. The model is consistent with structural information from vacuum-tunneling microscopy. It also provides simple explanations for stacking-fault-type features expected from Rutherford backscattering experiments and for similarities in the LEED and photoemission spectra of 2x1 and 7x7 surfaces.

  17. Photographic copy of 3 ½” x 5” glass lantern slide ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photographic copy of 3 ½” x 5” glass lantern slide no. 1 of map. Located in wooden pine box #23 in box 84 of 94 at the National Museum of American History, Smithsonian Institution, Archives Center, Work and industry Division, Washington, D.C. Original photographer, Milton R. Homes, Philadelphia, Pennsylvania. MAP SHOWING “SITES OF MAJOR BRIDGES ACROSS THE MISSISSIPPI BELOW ST. LOUIS”. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  18. Photographic copy of 3 ½” x 5” glass lantern slide ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photographic copy of 3 ½” x 5” glass lantern slide no. 2 of March 1925 drawing by Ralph Modjeski, Consulting Engineer. Located in wooden pine box #23 in box 84 of 94 at the National Museum of American History, Smithsonian Institution, Archives Center, Work and industry Division, Washington, D.C. MARCH 1925 DRAWING OF “PROPOSED BRIDGE OVER THE MISSISSIPPI RIVER NORTH OF NEW ORLEANS, LOUISIANA, PLAN “E”. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  19. Photographic copy of 3 ½” x 5” glass lantern slide ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Photographic copy of 3 ½” x 5” glass lantern slide no. 11 of map. Located in wooden pine box #23 in box 84 of 94 at the National Museum of American History, Smithsonian Institution, Archives Center, Work and industry Division, Washington, D.C. Original photographer, Milton R. Homes, Philadelphia, Pennsylvania. EARLY MAP OF “LOCATION OF NEW ORLEANS BRIDGE” AND THE VARIOUS RAILROADS SERVING THE GREATER NEW ORLEANS AREA. - Huey P. Long Bridge, Spanning Mississippi River approximately midway between nine & twelve mile points upstream from & west of New Orleans, Jefferson, Jefferson Parish, LA

  20. Fatigue life estimation program for Part 23 airplanes, `AFS.FOR`

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kaul, S.K.

    1993-12-31

    The purpose of this paper is to introduce to the general aviation industry a computer program which estimates the safe fatigue life of any Federal Aviation Regulation (FAR) Part 23 airplane. The algorithm uses the methodology (Miner`s Linear Cumulative Damage Theory) and the various data presented in the Federal Aviation Administration (FAA) Report No. AFS-120-73-2, dated May 1973. The program is written in FORTRAN 77 language and is executable on a desk top personal computer. The program prompts the user for the input data needed and provides a variety of options for its intended use. The program is envisaged tomore » be released through issuance of a FAA report, which will contain the appropriate comments, instructions, warnings and limitations.« less

  1. New one-pot synthesis of spiro[furo[2,3-d]pyrimidine-6,5'-pyrimidine]pentaones and their sulfur analogues.

    PubMed

    Jalilzadeh, Mohammad; Noroozi Pesyan, Nader; Rezaee, Fereshteh; Rastgar, Saeed; Hosseini, Yaser; Sahin, Ertan

    2011-08-01

    Reaction of barbituric acid (BA), 1,3-dimethyl barbituric acid (DMBA) and 2-thiobarbituric acid (TBA) with cyanogen bromide and various aldehydes in presence of triethylamine afforded a new class of heterocyclic stable 5-alkyl and/or 5-aryl-1H, 1'H-spiro[furo[2,3-d]pyrimidine-6,5'-pyrimidine]2,2',4,4',6'(3H,3'H,5H)-pentaones which are dimeric forms of barbiturate (uracil and thiouracil derivatives) at 0 °C to ambient temperatures. Structure elucidation is proved by X-ray crystallography, (1)H NMR, (13)C NMR, FT-IR, CHN and mass analyses techniques. Mechanisms of the formations are discussed.

  2. Triple-Quantum Filtered NMR Imaging of Sodium -23 in the Human Brain

    NASA Astrophysics Data System (ADS)

    Keltner, John Robinson

    In the past multiple-quantum filtered imaging of biexponential relaxation sodium-23 nuclei in the human brain has been limited by low signal to noise ratios; this thesis demonstrates that such imaging is feasible when using a modified gradient-selected triple-quantum filter at a repetition time which maximizes the signal to noise ratio. Nuclear magnetic resonance imaging of biexponential relaxation sodium-23 (^{23}Na) nuclei in the human brain may be useful for detecting ischemia, cancer, and pathophysiology related to manic-depression. Multiple -quantum filters may be used to selectively image biexponential relaxation ^{23}Na signals since these filters suppress single-exponential relaxation ^{23}Na signals. In this thesis, the typical repetition times (200 -300 ms) used for in vivo multiple-quantum filtered ^{23}Na experiments are shown to be approximately 5 times greater than the optimal repetition time which maximizes multiple-quantum filtered SNR. Calculations and experimental verification show that the gradient-selected triple-quantum (GS3Q) filtered SNR for ^ {23}Na in a 4% agarose gel increases by a factor of two as the repetition time decreases from 300 ms to 55 ms. It is observed that a simple reduction of repetition time also increases spurious single-quantum signals from GS3Q filtered experiments. Irreducible superoperator calculations have been used to design a modified GS3Q filter which more effectively suppresses the spurious single-quantum signals. The modified GS3Q filter includes a preparatory crusher gradient and two-step-phase cycling. Using the modified GS3Q filter and a repetition time of 70 ms, a three dimensional triple-quantum filtered image of a phantom modelling ^{23} Na in the brain was obtained. The phantom consisted of two 4 cm diameter spheres inside of a 8.5 cm x 7 cm ellipsoid. The two spheres contained 0.012 and 0.024 M ^{23}Na in 4% agarose gel. Surrounding the spheres and inside the ellipsoid was 0.03 M aqueous ^{23}Na. The image

  3. Validity and reliability of the Brazilian version of Yale-Brown obsessive compulsive scale-shopping version (YBOCS-SV).

    PubMed

    Leite, Priscilla Lourenço; Filomensky, Tatiana Zambrano; Black, Donald W; Silva, Adriana Cardoso

    2014-08-01

    The Yale-Brown Obsessive Compulsive Scale-Shopping Version (YBOCS-SV) is considered the gold standard in the assessment of shopping severity. It is designed to assess cognitions and behaviors relating to compulsive buying behavior. The present study aims to assess the validity of the Brazilian version of this scale. For the study, composed the sample 610 participants: 588 subjects of a general population and 22 compulsive buyers. Factorial analysis was performed to assess the relations and the correlation between the YBOCS-SV, the Compulsive Buying Scale (CBS), and Richmond Compulsive Buying Scale (RCBS), was assessed using Pearson coefficient, for study of convergent and divergent validity. Cronbach's alpha coefficients were used to assess internal consistency. The results show good to excellent psychometric parameters for the YBOCS-SV in its Brazilian version. With regard to correlations, the YBOCS-SV is inversely and proportionally correlated with CBS and the RCBS, indicating that the YBOCS-SV is an excellent instrument for screening compulsive buying. The YBOCS-SV presented high alpha coefficient of Cronbach's alpha (0.92), demonstrating good reliability. The Brazilian version of the YBOCS-SV is indicated to diagnose compulsive buying disorder, and likely use for the purposes intended in the Brazilian population. Copyright © 2014 Elsevier Inc. All rights reserved.

  4. Crocidolite asbestos and SV40 are cocarcinogens in human mesothelial cells and in causing mesothelioma in hamsters

    PubMed Central

    Kroczynska, Barbara; Cutrone, Rochelle; Bocchetta, Maurizio; Yang, Haining; Elmishad, Amira G.; Vacek, Pamela; Ramos-Nino, Maria; Mossman, Brooke T.; Pass, Harvey I.; Carbone, Michele

    2006-01-01

    Only a fraction of subjects exposed to asbestos develop malignant mesothelioma (MM), suggesting that additional factors may render some individuals more susceptible. We tested the hypothesis that asbestos and Simian virus (SV40) are cocarcinogens. Asbestos and SV40 in combination had a costimulatory effect in inducing ERK1/2 phosphorylation and activator protein-1 (AP-1) activity in both primary Syrian hamster mesothelial cells (SHM) and primary human mesothelial cells (HM). Ap-1 activity caused the expression and activation of matrix metalloprotease (MMP)-1 and MMP-9, which in turn led to cell invasion. Experiments using siRNA and chemical inhibitors confirmed the specificity of these results. The same effects were observed in HM and SHM. Experiments in hamsters showed strong cocarcinogenesis between asbestos and SV40: SV40 did not cause MM, asbestos caused MM in 20% of hamsters, and asbestos and SV40 together caused MM in 90% of hamsters. Significantly lower amounts of asbestos were sufficient to cause MM in animals infected with SV40. Our results indicate that mineral fibers and viruses can be cocarcinogens and suggest that lower amounts of asbestos may be sufficient to cause MM in individuals infected with SV40. PMID:16966607

  5. Synthesis and characterization of sodium vanadium oxide gels: the effects of water (n) and sodium (x) content on the electrochemistry of Na(x)V2O5·nH2O.

    PubMed

    Lee, Chia-Ying; Marschilok, Amy C; Subramanian, Aditya; Takeuchi, Kenneth J; Takeuchi, Esther S

    2011-10-28

    Sodium vanadium oxide gels, Na(x)V(2)O(5)·nH(2)O, of varying sodium content (0.12 < x < 0.32) were prepared by careful control of an ion exchange process. The water content (0.23 > n > 0.01) and interlayer spacing were found to be inversely proportional to the sodium level (x), thus control of sodium (x) content provided a direct, chimie douce approach for control of hydration level (n) and interlayer spacing, without the need for high temperature treatment to affect dehydration. Notably, the use of high temperatures to modify hydration levels can result in crystallization and collapse of the interlayer structure, highlighting the distinct advantage of our novel chimie douce synthesis strategy. Subsequent to synthesis and characterization, results from an electrochemical study of a series of Na(x)V(2)O(5)·nH(2)O samples highlight the significant impact of interlayer water on delivered capacity of the layered materials. Specifically, the sodium vanadium oxide gels with higher sodium content and lower water content provided higher capacities in lithium based cells, where capacity delivered to 2.0 V under C/20 discharge ranged from 170 mAh/g for Na(0.12)V(2)O(5)·0.23H(2)O to 300 mAh/g for Na(0.32)V(2)O(5)·0.01H(2)O. The capacity differences were maintained as the cells were cycled. This journal is © the Owner Societies 2011

  6. 23 CFR Appendix C to Part 1200 - ASSURANCES FOR TEEN TRAFFIC SAFETY PROGRAM

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false ASSURANCES FOR TEEN TRAFFIC SAFETY PROGRAM C APPENDIX C... STATE HIGHWAY SAFETY GRANT PROGRAMS Pt. 1200, App. C APPENDIX C TO PART 1200—ASSURANCES FOR TEEN TRAFFIC SAFETY PROGRAM State: Fiscal Year: The State has elected to implement a Teen Traffic Safety Program—a...

  7. 23 CFR Appendix C to Part 1200 - Assurances for Teen Traffic Safety Program

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Assurances for Teen Traffic Safety Program C Appendix C... STATE HIGHWAY SAFETY GRANT PROGRAMS Pt. 1200, App. C Appendix C to Part 1200—Assurances for Teen Traffic Safety Program State: Fiscal Year: The State has elected to implement a Teen Traffic Safety Program—a...

  8. Analysis of Patients' X-ray Exposure in 146 Percutaneous Radiologic Gastrostomies.

    PubMed

    Petersen, Tim-Ole; Reinhardt, Martin; Fuchs, Jochen; Gosch, Dieter; Surov, Alexey; Stumpp, Patrick; Kahn, Thomas; Moche, Michael

    2017-09-01

    Purpose  Analysis of patient´s X-ray exposure during percutaneous radiologic gastrostomies (PRG) in a larger population. Materials and Methods  Data of primary successful PRG-procedures, performed between 2004 and 2015 in 146 patients, were analyzed regarding the exposition to X-ray. Dose-area-product (DAP), dose-length-product (DLP) respectively, and fluoroscopy time (FT) were correlated with the used x-ray systems (Flatpanel Detector (FD) vs. Image Itensifier (BV)) and the necessity for periprocedural placement of a nasogastric tube. Additionally, the effective X-ray dose for PRG placement using fluoroscopy (DL), computed tomography (CT), and cone beam CT (CBCT) was estimated using a conversion factor. Results  The median DFP of PRG-placements under fluoroscopy was 163 cGy*cm 2 (flat panel detector systems: 155 cGy*cm 2 ; X-ray image intensifier: 175 cGy*cm 2 ). The median DLZ was 2.2 min. Intraprocedural placement of a naso- or orogastric probe (n = 68) resulted in a significant prolongation of the median DLZ to 2.5 min versus 2 min in patients with an already existing probe. In addition, dose values were analyzed in smaller samples of patients in which the PRG was placed under CBCT (n = 7, median DFP = 2635 cGy*cm 2 ), or using CT (n = 4, median DLP = 657 mGy*cm). Estimates of the median DFP and DLP showed effective doses of 0.3 mSv for DL-assisted placements (flat panel detector 0.3 mSv, X-ray image converter 0.4 mSv), 7.9 mSv using a CBCT - flat detector, and 9.9 mSv using CT. This corresponds to a factor 26 of DL versus CBCT, or a factor 33 of DL versus CT. Conclusion  In order to minimize X-ray exposure during PRG-procedures for patients and staff, fluoroscopically-guided interventions should employ flat detector systems with short transmittance sequences in low dose mode and with slow image frequency. Series recordings can be dispensed with. The intraprocedural placement of a naso- or orogastric probe

  9. 23 CFR 950.5 - Requirement to use electronic toll collection technology.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Requirement to use electronic toll collection technology... technology. (a) Any toll agency operating a toll facility pursuant to authority under a 1604 toll program... agency using electronic toll collection technology must develop and implement reasonable methods to...

  10. 23 CFR 950.5 - Requirement to use electronic toll collection technology.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Requirement to use electronic toll collection technology... technology. (a) Any toll agency operating a toll facility pursuant to authority under a 1604 toll program... agency using electronic toll collection technology must develop and implement reasonable methods to...

  11. 23 CFR 950.5 - Requirement to use electronic toll collection technology.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Requirement to use electronic toll collection technology... technology. (a) Any toll agency operating a toll facility pursuant to authority under a 1604 toll program... agency using electronic toll collection technology must develop and implement reasonable methods to...

  12. 23 CFR 950.5 - Requirement to use electronic toll collection technology.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Requirement to use electronic toll collection technology... technology. (a) Any toll agency operating a toll facility pursuant to authority under a 1604 toll program... agency using electronic toll collection technology must develop and implement reasonable methods to...

  13. 23 CFR 950.5 - Requirement to use electronic toll collection technology.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Requirement to use electronic toll collection technology... technology. (a) Any toll agency operating a toll facility pursuant to authority under a 1604 toll program... agency using electronic toll collection technology must develop and implement reasonable methods to...

  14. Structural and magnetic properties of sol-gel Co2xNi0.5-x Zn0.5-xFe2O4 thin films

    NASA Astrophysics Data System (ADS)

    Rebrov, Evgeny V.; Gao, Pengzhao; Verhoeven, Tiny M. W. G. M.; Schouten, Jaap C.; Kleismit, Richard; Turgut, Zafer; Kozlowski, Gregory

    2011-03-01

    Nanocrystalline Co2xNi0.5-xZn0.5-xFe2O4 (x=0-0.5) thin films have been synthesized with various grain sizes by a sol-gel method on polycrystalline silicon substrates. The morphology as well as magnetic and microwave absorption properties of the films calcined at 1073 K were studied using X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. All films were uniform without microcracks. The Co content in the Co-Ni-Zn films resulted in a grain size ranging from 15 to 32 nm while it ranged from 33 to 49 nm in the corresponding powders. Saturation and remnant magnetization increased with increase in grain size, while coercivity demonstrated a drop due to multidomain behavior of crystallites for a given value of x. Saturation magnetization increased and remnant magnetization had a maximum as a function of grain size independent of x. In turn, coercivity increased with x independent of grain size. Complex permittivity of the Co-Ni-Zn ferrite films was measured in the frequency range 2-15 GHz. The highest hysteretic heating rate in the temperature range 315-355 K was observed in CoFe2O4. The maximum absorption band shifted from 13 to 11 GHz as cobalt content increased from x=0.1 to 0.2.

  15. Flight Programs and X-ray Optics Development at MSFC

    NASA Technical Reports Server (NTRS)

    Gubarev, M.; Ramsey, B.; O'Dell, S. L.; Elsner, R.; Kilaru, K.; Atkins, C.; Swartz, D.; Gaskin, J.; Weisskopf, Martin

    2012-01-01

    The X-ray astronomy group at the Marshall Space Flight Center is developing electroformed nickel/cobalt x-ray optics for suborbital and orbital experiments. Suborbital instruments include the Focusing X-ray Solar Imager (FOXSI) and Micro-X sounding rocket experiments and the HERO balloon payload. Our current orbital program is the fabrication of a series of mirror modules for the Astronomical Roentgen Telescope (ART) to be launched on board the Russian-German Spectrum Roentgen Gamma Mission (SRG.) The details and status of these various programs are presented. A second component of our work is the development of fabrication techniques and optical metrology to improve the angular resolution of thin shell optics to the arcsecond-level. The status of these x-ray optics technology developments is also presented.

  16. Magnetism of hexagonal Mn{sub 1.5}X{sub 0.5}Sn (X = Cr, Mn, Fe, Co) nanomaterials

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fuglsby, R.; Kharel, P., E-mail: parashu.kharel@sdstate.edu; Nebraska Center for Materials and Nanoscience, University of Nebraska, Lincoln, Nebraska 68588

    2015-05-07

    Mn{sub 1.5}X{sub 0.5}Sn (X = Cr, Mn, Fe, Co) nanomaterials in the hexagonal Ni{sub 2}In-type crystal structure have been prepared using arc-melting and melt spinning. All the rapidly quenched Mn{sub 1.5}X{sub 0.5}Sn alloys show moderate saturation magnetizations with the highest value of 458 emu/cm{sup 3} for Mn{sub 1.5}Fe{sub 0.5}Sn, but their Curie temperatures are less than 300 K. All samples except the Cr containing one show spin-glass-like behavior at low temperature. The magnetic anisotropy constants calculated from the high-field magnetization curves at 100 K are on the order of 1 Merg/cm{sup 3}. The vacuum annealing of the ribbons at 550 °C significantly improved theirmore » magnetic properties with the Curie temperature increasing from 206 K to 273 K for Mn{sub 1.5}Fe{sub 0.5}Sn.« less

  17. The CfA Einstein Observatory extended deep X-ray survey

    NASA Technical Reports Server (NTRS)

    Primini, F. A.; Murray, S. S.; Huchra, J.; Schild, R.; Burg, R.

    1991-01-01

    All IPC exposures in the Einstein Extended Deep X-ray Survey program have been reanalyzed. The current survey covers about 2.3 sq deg with a typical limiting sensitivity of about 5 x 10 to the -14th ergs/sq cm/s in the energy range from 0.8-3.5 keV. A total of 25 IPC sources are detected above a threshold of 4.5 sigma. A total of 18 are detected independently in the HRI, leading to the identification of six with stars and 11 with extragalactic objects. The remaining sources are classified as extragalactic. The population of identified extragalactic objects is dominated by QSOs, with one or two possible clusters. The basic conclusions of the original survey remain unchanged.

  18. Structural And Electrical Properties oF (La{sub 0.5-x}Pr{sub x}Ba{sub 0.5})(Mn{sub 0.5}Ti{sub 0.5})O{sub 3} Perovskite

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Alias, Nor Hayati; Department of Physics, Faculty Science, University Putra of Malaysia; Shaari, Abdul Halim

    2010-01-05

    A single phase monoclinic new perovskite based titano-manganite (La{sub 0.5-x}Pr{sub x}Ba{sub 0.5})(Mn{sub 0.5}Ti{sub 0.5})O{sub 3} has been successfully prepared by ceramic solid-state technique at sintering temperature of 1300 deg. C. The concentration of Pr (Praseodymium), x, in molar proportion in A site has been varied as x = 0, 0.02 and 0.2. Analysis has been carried out to determine the electrical properties of the synthesized material at frequency ranging from 5 Hz to 1 MHz; and at temperature range between 25 deg. C to 200 deg. C. It is found that Pr addition promoted liquid phase sintering diffusion, porosity andmore » agglomeration formation at 1300 deg. C. Dual relaxation is observed in unsubstituted Pr sample x = 0 and high Pr substituted sample x = 0.2. This phenomenon was a combinational contribution from a quasi dc (QDC) or low frequency dispersion (LFD), two cole-cole relaxational responses and a resistor. While low concentrated Pr substituted sampled x = 0.02 shows a combinational contribution from a quasi dc (QDC) or low frequency dispersion (LFD), single cole-cole relaxational response and a resistor at room temperature. Pr substitution at x = 0(max 12000) and x = 0.2(max 16000) showed high dielectric values compared to low substituted sample x = 0.02. Variation of dielectric loss tangent (tan delta) are observed for all samples at temperature ranged studied.« less

  19. Influence of Fe doped on the magnetocaloric behavior of La_{{2}/{3}} Ca_{{1}/{3}} Mn1-x Fe x O3 compounds: a Monte Carlo simulation

    NASA Astrophysics Data System (ADS)

    Alzate-Cardona, J. D.; Barco-Rios, H.; Restrepo-Parra, E.

    2018-02-01

    The magnetocaloric behavior of La{2/{3}} Ca{1/{3}} Mn1-x Fe x O3 for x  =  0.00, 0.02, 0.03, 0.05, 0.07, 0.08 and 0.10 under the influence of an external magnetic field was simulated and analyzed. Simulations were carried out using the Monte Carlo method and the classical Heisenberg model under the Metropolis algorithm. These mixed valence manganites are characterized by having three types of magnetic ions corresponding to Mn4+≤ft(S=\\frac{3}{2}\\right) , which are bonded with Ca2+ , and Mneg3+ and Mneg\\prime3+ (S=2) , related to La3+ . The Fe ions were randomly included, replacing Mn ions. With this model, the magnetic entropy change, Δ S , in an isothermal process was determined. -Δ Sm showed maximum peaks around the paramagnetic-ferromagnetic transition temperature, which depends on Fe doping. Relative cooling power was computed for different Fe concentrations varying the magnetic applied field. Our model and results show that the Fe doping decreases the magnetocaloric effect in the La{2/{3}} Ca{1/{3}} Mn1-x Fe x O3, making this a bad candidate for magnetic refrigeration. The strong dependence of the magnetocaloric behavior on Fe doping and the external magnetic field in La{2/{3}} Ca{1/{3}} Mn1-x Fe x O3 can boost these materials for the future technological applications.

  20. Investigation of Relationship between High-energy X-Ray Sources and Photospheric and Helioseismic Impacts of X1.8 Solar Flare of 2012 October 23

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sharykin, I. N.; Zimovets, I. V.; Kosovichev, A. G.

    The X-class solar flare of 2012 October 23 generated continuum photospheric emission and a strong helioseismic wave (“sunquake”) that points to an intensive energy release in the dense part of the solar atmosphere. We study properties of the energy release with high temporal and spatial resolutions, using photospheric data from the Helioseismic Magnetic Imager (HMI) on board Solar Dynamics Observatory , and hard X-ray observations made by RHESSI . For this analysis we use level-1 HMI data (filtergrams), obtained by scanning the Fe i line (6731 Å) with the time cadence of ∼3.6 s and spatial resolution of ∼0.″5 permore » pixel. It is found that the photospheric disturbances caused by the flare spatially coincide with the region of hard X-ray emission but are delayed by ≲4 s. This delay is consistent with predictions of the flare hydrodynamics RADYN models. However, the models fail to explain the magnitude of variations observed by the HMI. The data indicate that the photospheric impact and helioseismic wave might be caused by the electron energy flux, which is substantially higher than that in the current flare radiative hydrodynamic models.« less

  1. Characterization of SV-40 Tag rats as a model to study prostate cancer

    PubMed Central

    2009-01-01

    Background Prostate cancer is the second most frequently diagnosed cancer in men. Animal models that closely mimic clinical disease in humans are invaluable tools in the fight against prostate cancer. Recently, a Simian Virus-40 T-antigen (SV-40 Tag) targeted probasin promoter rat model was developed. This model, however, has not been extensively characterized; hence we have investigated the ontogeny of prostate cancer and determined the role of sex steroid receptor and insulin-like growth factor-1 (IGF-1) signaling proteins in the novel SV-40 Tag rat. Methods The SV-40 Tag rat was histopathologically characterized for time to tumor development, incidence and multiplicity and in the ventral, dorsal, lateral and anterior lobes of the prostate. Immunoassay techniques were employed to measure cell proliferation, apoptosis, and sex steroid receptor and growth factor signaling-related proteins. Steroid hormone concentrations were measured via coated well enzyme linked immunosorbent assay (ELISA) kits. Results Prostatic intraepithelial neoplasia (PIN) and well-differentiated prostate cancer developed as early as 2 and 10 weeks of age, respectively in the ventral prostate (VP) followed by in the dorsolateral (DLP). At 8 weeks of age, testosterone and dihydrotestosterone (DHT) concentrations in SV-40 Tag rats were increased when compared to non-transgenic rats. High cell proliferation and apoptotic indices were found in VP and DLP of transgenic rats. Furthermore, we observed increased protein expression of androgen receptor, IGF-1, IGF-1 receptor, and extracellular signal-regulated kinases in the prostates of SV-40 Tag rats. Conclusion The rapid development of PIN and prostate cancer in conjunction with the large prostate size makes the SV-40 Tag rat a useful model for studying prostate cancer. This study provides evidence of the role of sex steroid and growth factor proteins in prostate cancer development and defines appropriate windows of opportunity for preclinical

  2. 49 CFR 23.21 - Who must submit an ACDBE program to FAA, and when?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... program, you may implement one plan for all your locations. If you do so, you must establish a separate ACDBE goal for each location. (d) If you make any significant changes to your ACDBE program at any time... DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS ACDBE Programs § 23.21 Who must submit an ACDBE program...

  3. 23 CFR 1.5 - Information furnished by State highway departments.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Information furnished by State highway departments. 1.5 Section 1.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL MANAGEMENT AND ADMINISTRATION GENERAL § 1.5 Information furnished by State highway departments. At the request of the...

  4. 23 CFR 1.5 - Information furnished by State highway departments.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Information furnished by State highway departments. 1.5 Section 1.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL MANAGEMENT AND ADMINISTRATION GENERAL § 1.5 Information furnished by State highway departments. At the request of the...

  5. 23 CFR 1.5 - Information furnished by State highway departments.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Information furnished by State highway departments. 1.5 Section 1.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL MANAGEMENT AND ADMINISTRATION GENERAL § 1.5 Information furnished by State highway departments. At the request of the...

  6. 23 CFR 1.5 - Information furnished by State highway departments.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Information furnished by State highway departments. 1.5 Section 1.5 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION GENERAL MANAGEMENT AND ADMINISTRATION GENERAL § 1.5 Information furnished by State highway departments. At the request of the...

  7. Fabrication of Lead-free (K0.5Na0.5)1- x Ag x NbO3 Ferroelectric Ceramics and Their Dielectric Properties

    NASA Astrophysics Data System (ADS)

    Byun, Jaeduk; Hyun, June Won; Kim, Yeon Jung; Bobor, Kristóf

    2018-03-01

    In this study, lead-free (K0.5Na0.5)1- x Ag x NbO3 ( x = 0.00, 0.10, 0.15, 0.20, 0.25, and 0.30) ferroelectric ceramics were fabricated using solid-state synthesis without A-site and B-site manufacturing step. The (K0.5Na0.5)1- x Ag x NbO3 ceramics were sintered at 1110 °C for 4 h after calcination at 800 °C for 3 h. The sintered sample was dense, and the grain size was 1.02 7.8 μm. For x ≤ 0.2, the sintered ceramic samples had a single perovskite structure. The temperature dependence of the dielectric constant in the (K0.5Na0.5)1- x Ag x NbO3 was measured at 1 kHz using an LCR meter. The high dielectric constant properties could be obtained in (K0.5Na0.5)1- x Ag x NbO3 ceramics. The orthorhombic-to-tetragonal phase transition temperature and ferroelectric Curie temperature decreased linearly with increasing mole fraction of the Ag content. The Curie temperature shifted from 393 °C for (K0.5Na0.5)NbO3 ceramics to 317 °C for (K0.5Na0.5)0.7Ag0.3NbO3 ceramics. The maximum dielectric constant was 8930 at 330 °C in the (K0.5Na0.5)0.8Ag0.2NbO3 ceramics.

  8. Aromatic derivatives of 1H-2,3-dihydropyrazolo(4,5-b)-1,5-diazepine

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Orlov, V.D.; Kiroga, Kh.; Kolos, N.N.

    1987-09-01

    Aromatic derivatives of 1H-2,3-dihydropyrazole(4,5-b)-1,5-diazepine were obtained by the reaction of 1-phenyl-3-methyl-4,5-diaminopyrazole with chalcones and acetylarenes, catalyzed by acetic or sulfuric acid. The seven-membered ring in these compounds has a conformation of the boat type. The IR, UV, PMR, and mass spectra of the compounds are discussed.

  9. Topological phases in (Na2O)x (P2O5)100-x glasses

    NASA Astrophysics Data System (ADS)

    Mohanty, Chandi; Chbeir, Ralph; Czaja, Andrew; Chen, Ping; Boolchand, Punit

    We have synthesized titled glasses in the 0 <x <0.50 range of soda paying special attention to their dryness. Pure P2O5 glass was synthesized by flash evaporation of bulk powder in a quartz tube as it was pumped in several attempts, and the variation of Tg and enthalpy of relaxation (ΔHnr) measured for each attempt. These data show that as the glass got drier, Tg increased to 431°C and ΔHnr became miniscule. At higher soda content (x >20%), Tg(x) increased steadily, but with appearance of a local maximum near x = 37.5%. On the other hand ΔHnr term, revealed a Trapezoidal-like minimum in the 32.5 % <x <42.5% range, suggestive of a reversibility window or the isostatically rigid Intermediate Phase, with glasses at x >42.5% in the flexible phase while those in the 20% <x <32.5% range in the stressed rigid phase. We have also obtained Raman scattering, IR reflectance and fragility index measurements on the present glasses, and these will discussed with recent results in the field.

  10. SV40 Utilizes ATM Kinase Activity to Prevent Non-homologous End Joining of Broken Viral DNA Replication Products

    PubMed Central

    Sowd, Gregory A.; Mody, Dviti; Eggold, Joshua; Cortez, David; Friedman, Katherine L.; Fanning, Ellen

    2014-01-01

    Simian virus 40 (SV40) and cellular DNA replication rely on host ATM and ATR DNA damage signaling kinases to facilitate DNA repair and elicit cell cycle arrest following DNA damage. During SV40 DNA replication, ATM kinase activity prevents concatemerization of the viral genome whereas ATR activity prevents accumulation of aberrant genomes resulting from breakage of a moving replication fork as it converges with a stalled fork. However, the repair pathways that ATM and ATR orchestrate to prevent these aberrant SV40 DNA replication products are unclear. Using two-dimensional gel electrophoresis and Southern blotting, we show that ATR kinase activity, but not DNA-PKcs kinase activity, facilitates some aspects of double strand break (DSB) repair when ATM is inhibited during SV40 infection. To clarify which repair factors associate with viral DNA replication centers, we examined the localization of DSB repair proteins in response to SV40 infection. Under normal conditions, viral replication centers exclusively associate with homology-directed repair (HDR) and do not colocalize with non-homologous end joining (NHEJ) factors. Following ATM inhibition, but not ATR inhibition, activated DNA-PKcs and KU70/80 accumulate at the viral replication centers while CtIP and BLM, proteins that initiate 5′ to 3′ end resection during HDR, become undetectable. Similar to what has been observed during cellular DSB repair in S phase, these data suggest that ATM kinase influences DSB repair pathway choice by preventing the recruitment of NHEJ factors to replicating viral DNA. These data may explain how ATM prevents concatemerization of the viral genome and promotes viral propagation. We suggest that inhibitors of DNA damage signaling and DNA repair could be used during infection to disrupt productive viral DNA replication. PMID:25474690

  11. Biodistribution and Radiation Dosimetry for the Novel SV2A Radiotracer [(18)F]UCB-H: First-in-Human Study.

    PubMed

    Bretin, F; Bahri, M A; Bernard, C; Warnock, G; Aerts, J; Mestdagh, N; Buchanan, T; Otoul, C; Koestler, F; Mievis, F; Giacomelli, F; Degueldre, C; Hustinx, R; Luxen, A; Seret, A; Plenevaux, A; Salmon, E

    2015-08-01

    [(18)F]UCB-H is a novel radiotracer with a high affinity for synaptic vesicle glycoprotein 2A (SV2A), a protein expressed in synaptic vesicles. SV2A is the binding site of levetiracetam, a "first-in-class" antiepileptic drug with a distinct but still poorly understood mechanism of action. The objective of this study was to determine the biodistribution and radiation dosimetry of [(18)F]UCB-H in a human clinical trial and to establish injection limits according to biomedical research guidelines. Additionally, the clinical radiation dosimetry results were compared to estimations in previously published preclinical data. Dynamic whole body positron emission tomography/X-ray computed tomography (PET/CT) imaging was performed over approximately 110 min on five healthy male volunteers after injection of 144.5 ± 7.1 MBq (range, 139.1-156.5 MBq) of [(18)F]UCB-H. Major organs were delineated on CT images, and time-activity curves were obtained from co-registered dynamic PET emission scans. The bladder could only be delineated on PET images. Time-integrated activity coefficients were calculated as area under the curve using trapezoidal numerical integration. Urinary excretion data based on PET activities including voiding was also simulated using the dynamic bladder module of OLINDA/EXM. The radiation dosimetry was calculated using OLINDA/EXM. The effective dose to the OLINDA/EXM 70-kg standard male was 1.54 × 10(-2) ± 6.84 × 10(-4) millisieverts (mSv)/MBq, with urinary bladder wall, gallbladder wall, and the liver receiving the highest absorbed dose. The brain, the tracer's main organ of interest, received an absorbed dose of 1.89 × 10(-2) ± 2.32 × 10(-3) mGy/MBq. This first human dosimetry study of [(18)F]UCB-H indicated that the tracer shows similar radiation burdens to widely used common clinical tracers. Single injections of at maximum 672 MBq for US practice and 649 MBq for European practice keep radiation exposure below

  12. Dynamics of the Upper Atmosphere X-ray Emission during the 23rd Solar Cycle

    NASA Astrophysics Data System (ADS)

    Pugacheva, Galina; Gusev, Anatoly; Martin, Inácio M.; Spjeldvik, Walther

    Long-term observations with the RPS-1instrument on the CORONAS-F satellite (July 2001 to December 2005) permitted the evaluation of the low energy 3.0-31.5 keV X-ray emission flux radiated by the upper nocturnal atmosphere. This emission mostly results from the bremsstrahlung radiation from magnetospheric electrons. The entire nocturnal atmosphere emits energy in the range of 3 to 5 keV, especially in the southern hemisphere, over the Pacific and Indian ocean areas. In the northern hemisphere, the brightest emission from the atmo-sphere is observed at high latitudes in the region of Earth's radiation belt (ERB). In lower northern latitudes, the X-ray emission intensity is rather weak especially during the summer, and on 5-8 keV maps there are regions where there are no discernible emissions. At energies higher than 8 keV, only areas over the South-Atlantic magnetic anomaly and ERB at high latitudes are distinctly observed. This emission is produced by X-rays arising from interactions of ERB particles, descending to the altitude of 500 km in their bounce motion with the am-bient atmospheric matter, and by direct ERB particles passing through the lateral walls and entrance window of the detector (electrons with energies higher than 100 keV and protons with energies higher than 3 MeV). In order to determine the source mechanisms of soft X-rays in the energy range 3 to 8 keV from regions in the ERB, we studied the relationship between the seasonal variation of the X-ray atmospheric radiation and phases of the solar activity cycle. The global monthly, six-monthly, and yearly-averaged X-ray flux distributions were statistically determined for the five-year duration of the CORONAS-F mission. From these distributions, it is possible to infer about the influence of the phase of the solar activity and seasonal effects on the fluxes with energy in the range of 3 to 8 keV. Analysis of these data revealed important regularities in the behavior of this emission. We noted that

  13. 77 FR 38124 - Inspector General; Line of Succession Designation, No. 23-C, Revision 5

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-26

    ... SMALL BUSINESS ADMINISTRATION Inspector General; Line of Succession Designation, No. 23-C, Revision 5 This document replaces and supersedes ``Delegation of Authority and Line of Succession No. 23-C, Revision 4.'' Line of Succession Designation, No. 23-C, Revision 5: Effective immediately, the Inspector...

  14. Concurrent ionic migration and electronic effects at the memristive TiO x /La1/3Ca2/3MnO3-x interface

    NASA Astrophysics Data System (ADS)

    Román Acevedo, W.; Ferreyra, C.; Sánchez, M. J.; Acha, C.; Gay, R.; Rubi, D.

    2018-03-01

    The development of reliable redox-based resistive random-access memory devices requires understanding and disentangling concurrent effects present at memristive interfaces. We report on the fabrication and electrical characterization of TiO x /La1/3Ca2/3MnO3-x microstructured interfaces and on the modeling of their memristive behavior. We show that a careful tuning of the applied external electrical stimuli allows controlling the redox process between both layers, obtaining multilevel non-volatile resistance states. We simulate the oxygen vacancies dynamics at the interface between both oxides, and successfully reproduce the experimental electrical behavior after the inclusion of an electronic effect, related to the presence of an n-p diode at the interface. The formation of the diode is due to the n- and p-character of TiO x and La1/3Ca2/3MnO3-x , respectively. Our analysis indicates that oxygen vacancies migration between both layers is triggered after the diode is polarized either in forward mode or in reverse mode above breakdown. Electrical measurements at different temperatures suggest that the diode can be characterized as Zener-type. The advantages of our junctions for their implementation in RRAM devices are finally discussed.

  15. Solar x ray astronomy rocket program

    NASA Technical Reports Server (NTRS)

    1990-01-01

    The dynamics were studied of the solar corona through the imaging of large scale coronal structures with AS&E High Resolution Soft X ray Imaging Solar Sounding Rocket Payload. The proposal for this program outlined a plan of research based on the construction of a high sensitivity X ray telescope from the optical and electronic components of the previous flight of this payload (36.038CS). Specifically, the X ray sensitive CCD camera was to be placed in the prime focus of the grazing incidence X ray mirror. The improved quantum efficiency of the CCD detector (over the film which had previously been used) allows quantitative measurements of temperature and emission measure in regions of low x ray emission such as helmet streamers beyond 1.2 solar radii or coronal holes. Furthermore, the improved sensitivity of the CCD allows short exposures of bright objects to study unexplored temporal regimes of active region loop evolution.

  16. One-pot tandem Ugi-4CR/S(N)Ar approach to highly functionalized quino[2,3-b][1,5]benzoxazepines.

    PubMed

    Ghandi, Mehdi; Zarezadeh, Nahid; Abbasi, Alireza

    2016-05-01

    We have developed a convenient and facile method for the synthesis of functionalized diverse quino[2,3-b][1,5]benzoxazepines. These new compounds were synthesized through a one-pot sequential Ugi-4CR/base-free intramolecular aromatic nucleophilic substitution (S(N)Ar) reaction in moderate to good yields from readily available starting materials. Structural confirmation of the products is confirmed by analytical data and X-ray crystallography.

  17. Chimeric SV40 virus-like particles induce specific cytotoxicity and protective immunity against influenza A virus without the need of adjuvants

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kawano, Masaaki; Morikawa, Katsuma; Suda, Tatsuya

    Virus-like particles (VLPs) are a promising vaccine platform due to the safety and efficiency. However, it is still unclear whether polyomavirus-based VLPs are useful for this purpose. Here, we attempted to evaluate the potential of polyomavirus VLPs for the antiviral vaccine using simian virus 40 (SV40). We constructed chimeric SV40-VLPs carrying an HLA-A{sup ⁎}02:01-restricted, cytotoxic T lymphocyte (CTL) epitope derived from influenza A virus. HLA-A{sup ⁎}02:01-transgenic mice were then immunized with the chimeric SV40-VLPs. The chimeric SV40-VLPs effectively induced influenza-specific CTLs and heterosubtypic protection against influenza A viruses without the need of adjuvants. Because DNase I treatment of the chimericmore » SV40-VLPs did not disrupt CTL induction, the intrinsic adjuvant property may not result from DNA contaminants in the VLP preparation. In addition, immunization with the chimeric SV40-VLPs generated long-lasting memory CTLs. We here propose that the chimeric SV40-VLPs harboring an epitope may be a promising CTL-based vaccine platform with self-adjuvant properties. - Highlights: • We constructed chimeric SV40-VLPs carrying an influenza virus-derived CTL epitope. • Chimeric SV40-VLPs induce influenza-specific CTLs in mice without adjuvants. • Chimeric SV40-VLPs induce heterosubtypic protection against influenza A viruses. • Chimeric SV40-VLPs induce long-lasting memory CTLs. • Chimeric SV40-VLPs is a promising vaccine platform with self-adjuvant properties.« less

  18. NEW OBSERVATIONS OF THE SOLAR 0.5–5 KEV SOFT X-RAY SPECTRUM

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Caspi, Amir; Woods, Thomas N.; Warren, Harry P.

    2015-03-20

    The solar corona is orders of magnitude hotter than the underlying photosphere, but how the corona attains such high temperatures is still not understood. Soft X-ray (SXR) emission provides important diagnostics for thermal processes in the high-temperature corona, and is also an important driver of ionospheric dynamics at Earth. There is a crucial observational gap between ∼0.2 and ∼4 keV, outside the ranges of existing spectrometers. We present observations from a new SXR spectrometer, the Amptek X123-SDD, which measured the spatially integrated solar spectral irradiance from ∼0.5 to ∼5 keV, with ∼0.15 keV FWHM resolution, during sounding rocket flights onmore » 2012 June 23 and 2013 October 21. These measurements show that the highly variable SXR emission is orders of magnitude greater than that during the deep minimum of 2009, even with only weak activity. The observed spectra show significant high-temperature (5–10 MK) emission and are well fit by simple power-law temperature distributions with indices of ∼6, close to the predictions of nanoflare models of coronal heating. Observations during the more active 2013 flight indicate an enrichment of low first-ionization potential elements of only ∼1.6, below the usually observed value of ∼4, suggesting that abundance variations may be related to coronal heating processes. The XUV Photometer System Level 4 data product, a spectral irradiance model derived from integrated broadband measurements, significantly overestimates the spectra from both flights, suggesting a need for revision of its non-flare reference spectra, with important implications for studies of Earth ionospheric dynamics driven by solar SXRs.« less

  19. The Hyper-X Flight Systems Validation Program

    NASA Technical Reports Server (NTRS)

    Redifer, Matthew; Lin, Yohan; Bessent, Courtney Amos; Barklow, Carole

    2007-01-01

    For the Hyper-X/X-43A program, the development of a comprehensive validation test plan played an integral part in the success of the mission. The goal was to demonstrate hypersonic propulsion technologies by flight testing an airframe-integrated scramjet engine. Preparation for flight involved both verification and validation testing. By definition, verification is the process of assuring that the product meets design requirements; whereas validation is the process of assuring that the design meets mission requirements for the intended environment. This report presents an overview of the program with emphasis on the validation efforts. It includes topics such as hardware-in-the-loop, failure modes and effects, aircraft-in-the-loop, plugs-out, power characterization, antenna pattern, integration, combined systems, captive carry, and flight testing. Where applicable, test results are also discussed. The report provides a brief description of the flight systems onboard the X-43A research vehicle and an introduction to the ground support equipment required to execute the validation plan. The intent is to provide validation concepts that are applicable to current, follow-on, and next generation vehicles that share the hybrid spacecraft and aircraft characteristics of the Hyper-X vehicle.

  20. A monoclonal antibody against SV40 large T antigen (PAb416) does not label Merkel cell carcinoma.

    PubMed

    Pelletier, Daniel J; Czeczok, Thomas W; Bellizzi, Andrew M

    2018-07-01

    Merkel cell carcinoma represents poorly differentiated neuroendocrine carcinoma of cutaneous origin. In most studies, the vast majority of Merkel cell carcinomas are Merkel cell polyomavirus (MCPyV)-associated. SV40 polyomavirus immunohistochemistry is typically used in the diagnosis of other polyomavirus-associated diseases, including tubulointerstitial nephritis and progressive multifocal leukoencephalopathy, given cross-reactivity with BK and JC polyomaviruses. MCPyV-specific immunohistochemistry is commercially available, but, if antibodies against SV40 also cross-reacted with MCPyV, that would be advantageous from a resource-utilisation perspective. Tissue microarrays were constructed from 39 Merkel cell carcinomas, 24 small-cell lung carcinomas, and 18 extrapulmonary visceral small-cell carcinomas. SV40 large T antigen immunohistochemistry (clone PAb416) was performed; MCPyV large T antigen immunohistochemistry (clone CM2B4) had been previously performed. UniProt was used to compare the amino acid sequences of the SV40, BK, JC and MCPyV large T antigens, focusing on areas recognised by the PAb416 and CM2B4 clones. SV40 immunohistochemistry was negative in all tumours; MCPyV immunohistochemistry was positive in 38% of Merkel cell carcinomas and in 0% of non-cutaneous poorly differentiated neuroendocrine carcinomas. UniProt analysis revealed a high degree of similarity between SV40, BK, and JC viruses in the region recognised by PAb416. There was less homology between SV40 and MCPyV in this region, which was also interrupted by two long stretches of amino acids unique to MCPyV. The CM2B4 clone recognises a unique epitope in one of these stretches. The PAb416 antibody against the SV40 large T antigen does not cross-react with MCPyV large T antigen, and thus does not label Merkel cell carcinoma. © 2018 John Wiley & Sons Ltd.

  1. Magnetic behaviors in melt spun Fe{sub 52−x}Mn{sub 23+x}Ga{sub 25} (x = 0–3) ribbons

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shih, C. W.; Lee, Y. I.; Chang, W. C., E-mail: phywcc@ccu.edu.tw

    2014-05-07

    The effect of volume fraction of B2-type ferromagnetic (FM) phase on magnetic behavior of melt-spun Fe{sub 52−x}Mn{sub 23+x}Ga{sub 25} (x = 0, 1, 2, and 3) ribbons has been investigated. X-ray diffraction (XRD) results show that the volume fraction of the B2 phase is decreased, accompanied by the increased antiferromagnetic (AFM) fcc phase, with increasing x. The magnetization isotherms of these ribbons at different temperatures demonstrate that there exists a critical field H{sub CR} from AFM to FM state, and the H{sub CR} decreases and vanishes finally with increasing temperature. High exchange bias field (H{sub EB}) at 10 K through a cooling fieldmore » at H{sub CR} from 300 K could be found and correlated to the volume fraction of B2-type FM phase. Both H{sub CR} and H{sub EB} are increased with decreasing the amount of B2-type FM phase. The H{sub CR} is increased from 20 kOe for x = 0 to 50 kOe for x = 3, and H{sub EB} is increased from 0.9 kOe for x = 0 to 2.5 kOe for x = 2. The latter phenomenon might be attributed to the change of exchange interactions at FM/AFM interfaces due to the change of proportion of FM and AFM phase at low temperature.« less

  2. X-ray binaries

    NASA Technical Reports Server (NTRS)

    1976-01-01

    Satellite X-ray experiments and ground-based programs aimed at observation of X-ray binaries are discussed. Experiments aboard OAO-3, OSO-8, Ariel 5, Uhuru, and Skylab are included along with rocket and ground-based observations. Major topics covered are: Her X-1, Cyg X-3, Cen X-3, Cyg X-1, the transient source A0620-00, other possible X-ray binaries, and plans and prospects for future observational programs.

  3. Pressure-induced phase transition in La 1 – x Sm x O 0.5 F 0.5 BiS 2

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fang, Y.; Yazici, D.; White, B. D.

    Electrical resistivity measurements on La 1–xSm xO 0.5F 0.5BiS 2 (x = 0.1, 0.3, 0.6, 0.8) have been performed under applied pressures up to 2.6 GPa from 2 K to room temperature. The superconducting transition temperature T c of each sample significantly increases at a Sm-concentration dependent pressure P t, indicating a pressure-induced phase transition from a low-T c to a high-T c phase. At ambient pressure, T c increases dramatically from 2.8 K at x = 0.1 to 5.4 K at x = 0.8; however, the T c values at P > P t decrease slightly with x andmore » P t shifts to higher pressures with Sm substitution. In the normal state, semiconducting-like behavior is suppressed and metallic conduction is induced with increasing pressure in all of the samples. Furthermore, these results suggest that the pressure dependence of T c for the BiS 2-based superconductors is related to the lattice parameters at ambient pressure and enable us to estimate the evolution of T c for SmO 0.5F 0.5BiS 2 under pressure.« less

  4. Pressure-induced phase transition in La 1 – x Sm x O 0.5 F 0.5 BiS 2

    DOE PAGES

    Fang, Y.; Yazici, D.; White, B. D.; ...

    2015-09-15

    Electrical resistivity measurements on La 1–xSm xO 0.5F 0.5BiS 2 (x = 0.1, 0.3, 0.6, 0.8) have been performed under applied pressures up to 2.6 GPa from 2 K to room temperature. The superconducting transition temperature T c of each sample significantly increases at a Sm-concentration dependent pressure P t, indicating a pressure-induced phase transition from a low-T c to a high-T c phase. At ambient pressure, T c increases dramatically from 2.8 K at x = 0.1 to 5.4 K at x = 0.8; however, the T c values at P > P t decrease slightly with x andmore » P t shifts to higher pressures with Sm substitution. In the normal state, semiconducting-like behavior is suppressed and metallic conduction is induced with increasing pressure in all of the samples. Furthermore, these results suggest that the pressure dependence of T c for the BiS 2-based superconductors is related to the lattice parameters at ambient pressure and enable us to estimate the evolution of T c for SmO 0.5F 0.5BiS 2 under pressure.« less

  5. Synthesis and electrochemical characteristics of LiCr xNi 0.5- xMn 1.5O 4 spinel as 5 V cathode materials for lithium secondary batteries

    NASA Astrophysics Data System (ADS)

    Hong, Ki-Joo; Sun, Yang-Kook

    A series of electrochemical spinel compounds, LiCr xNi 0.5- xMn 1.5O 4 ( x=0, 0.1, 0.3), are synthesized by a sol-gel method and their electrochemical properties are characterized in the voltage range of 3.5-5.2 V. Electrochemical data for LiCr xNi 0.5- xMn 1.5O 4 electrodes show two reversible plateaus at 4.9 and 4.7 V. The 4.9 V plateau is related to the oxidation of chromium while the 4.7 V plateau is ascribed to the oxidation of nickel. The LiCr 0.1Ni 0.4Mn 1.5O 4 electrode delivers a high initial capacity of 152 mAh g -1 with excellent cycleability. The excellent capacity retention of the LiCr 0.1Ni 0.4Mn 1.5O 4 electrode is largely attributed to structural stabilization which results from co-doping (chromium and nickel) and increased theoretical capacity due to substitution of chromium.

  6. Estimating surface reflectance from Himawari-8/AHI reflectance channels Using 6SV

    NASA Astrophysics Data System (ADS)

    Lee, Kyeong-sang; Choi, Sungwon; Seo, Minji; Seong, Noh-hun; Han, Kyung-soo

    2017-04-01

    TOA (Top Of Atmospheric) reflectance observed by satellite is modified by the influence of atmosphere such as absorbing and scattering by molecular and gasses. Removing TOA reflectance attenuation which is caused by the atmospheric is essential. surface reflectance with compensated atmospheric effects used as important input data for land product such as Normalized Difference Vegetation Index (NDVI), Land Surface Albedo (LSA) and etc. In this study, we Second Simulation of a Satellite Signal in the Solar Spectrum Vector (6SV) Radiative Transfer Model (RTM) for atmospheric correction and estimating surface reflectance from Himawari-8/Advanced Himawari Imager (AHI) reflectance channels. 6SV has the advantage that it has high accuracy by performing the atmospheric correction by dividing the width of the satellite channel by 2.5 nm, but it is slow to use in the operation. So, we use LUT approach to reduce the computation time and avoid the intensive calculation required for retrieving surface reflectance. Estimated surface reflectance data were compared with PROBA-V S1 data to evaluate the accuracy. As a result Root Mean Square Error (RMSE) and bias were about 0.05 and -0.02. It is considered that this error is due to the difference of angle component and Spectral Response Function (SRF) of each channel.

  7. Monoclinic Sr(1-x)Na(x)SiO(3-0.5x): new superior oxide ion electrolytes.

    PubMed

    Singh, Preetam; Goodenough, John B

    2013-07-10

    Oxide ion electrolytes determine the temperature of operation of solid oxide fuel cells, oxygen separation membranes, and oxygen sensors. There is a strong incentive to lower their operating temperatures, in a solid oxide fuel cell, for example, from Top > 800 °C to Top ≈ 500 °C. The use of low-cost Na(+) rather than K(+) as the dopant in monoclinic SrSiO3 (C12/C1) is shown to provide a larger solid solution range (0 < x ≤ 0.45) in Sr1-xNaxSiO3-0.5x and to achieve an oxide ion conductivity σo ≥ 10(-2) S·cm(-1) by 525 °C as a result of lowering the temperature of a smooth transition to full disorder of the mobile oxide ions. The Sr1-xNaxSiO3-0.5x electrolytes are much less hygroscopic than Sr1-xKxSiO3-0.5x and are stable with a nickel composite anode in 5% H2/Ar as well as with cathodes such as La1-xSrxMnO3-δ and Sr0.7Y0.3CoO3-δ in air, which makes them candidate electrolytes for intermediate-temperature solid oxide fuel cells or for other applications of oxide ion electrolytes.

  8. X-40A Free Flight #5

    NASA Technical Reports Server (NTRS)

    2001-01-01

    X-40A Free Flight #5. The unpowered X-40A, an 85 percent scale risk reduction version of the proposed X-37, proved the capability of an autonomous flight control and landing system in a series of glide flights at NASA's Dryden Flight Research Center in California. NASA's Marshall Space Flight Center in Huntsville, Alabama, manages the X-37 project. At Dryden, the X-40A underwent a series of ground and air tests to reduce possible risks to the larger X-37, including drop tests from a helicopter to check guidance and navigation systems planned for use in the X-37. The X-37 is designed to demonstrate technologies in the orbital and reentry environments for next-generation reusable launch vehicles that will increase both safety and reliability, while reducing launch costs from $10,000 per pound to $1,000 per pound. The X-37, carried into orbit by the Space Shuttle, is planned to fly two orbital missions to test reusable launch vehicle technologies.

  9. 23 CFR 1200.23 - Vouchers and project agreements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 23 Highways 1 2012-04-01 2012-04-01 false Vouchers and project agreements. 1200.23 Section 1200.23... Implementation and Management of the Highway Safety Program § 1200.23 Vouchers and project agreements. Each State... rate (or Special matching writeoff used, i.e., sliding scale rate authorized under 23 U.S.C. 120(a...

  10. 23 CFR 1200.23 - Vouchers and project agreements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Vouchers and project agreements. 1200.23 Section 1200.23... Implementation and Management of the Highway Safety Program § 1200.23 Vouchers and project agreements. Each State... rate (or Special matching writeoff used, i.e., sliding scale rate authorized under 23 U.S.C. 120(a...

  11. 23 CFR 1200.23 - Vouchers and project agreements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Vouchers and project agreements. 1200.23 Section 1200.23... Implementation and Management of the Highway Safety Program § 1200.23 Vouchers and project agreements. Each State... rate (or Special matching writeoff used, i.e., sliding scale rate authorized under 23 U.S.C. 120(a...

  12. Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line

    PubMed Central

    Song, Xiu-Guang; Bian, Peng-Fei; Yu, Shu-Li; Zhao, Xiu-Hua; Xu, Wei; Bu, Xue-Hui; Li, Xia; Ma, Li-Xian

    2013-01-01

    AIM: To investigate the expression of the hepatitis B virus (HBV) 1.3-fold genome plasmid (pHBV1.3) in an immortalized mouse hepatic cell line induced by SV40 T-antigen (SV40T) expression. METHODS: Mouse hepatic cells were isolated from mouse liver tissue fragments from 3-5 d old Kunming mice by the direct collagenase digestion method and cultured in vitro. The pRSV-T plasmid was transfected into mouse hepatic cells to establish an SV40LT-immortalized mouse hepatic cell line. The SV40LT-immortalized mouse hepatic cells were identified and transfected with the pHBV1.3 plasmid. The levels of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) in the supernatant were determined by an electrochemiluminescence immunoassay at 24, 48, 72 and 96 h after transfection. The expressions of HBsAg and hepatitis B c antigen (HBcAg) in the cells were investigated by indirect immunofluorescence analysis. The presence of HBV DNA replication intermediates in the transfected cells and viral particles in the supernatant of the transfected cell cultures was monitored using the Southern hybridization assay and transmission electronic microscopy, respectively. RESULTS: The pRSV-T plasmid was used to immortalize mouse hepatocytes and an SV40LT-immortalized mouse hepatic cell line was successfully established. SV40LT-immortalized mouse hepatic cells have the same morphology and growth characteristics as primary mouse hepatic cells can be subcultured and produce albumin and cytokeratin-18 in vitro. Immortalized mouse hepatic cells did not show the characteristics of tumor cells, as alpha-fetoprotein levels were comparable (0.58 ± 0.37 vs 0.61 ± 0.31, P = 0.37). SV40LT-immortalized mouse hepatic cells were then transfected with the pHBV1.3 plasmid, and it was found that the HBV genome replicated in SV40LT-immortalized mouse hepatic cells. The levels of HBsAg and HBeAg continuously increased in the supernatant after the transfection of pHBV1.3, and began to decrease 72 h

  13. Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line.

    PubMed

    Song, Xiu-Guang; Bian, Peng-Fei; Yu, Shu-Li; Zhao, Xiu-Hua; Xu, Wei; Bu, Xue-Hui; Li, Xia; Ma, Li-Xian

    2013-11-28

    To investigate the expression of the hepatitis B virus (HBV) 1.3-fold genome plasmid (pHBV1.3) in an immortalized mouse hepatic cell line induced by SV40 T-antigen (SV40T) expression. Mouse hepatic cells were isolated from mouse liver tissue fragments from 3-5 d old Kunming mice by the direct collagenase digestion method and cultured in vitro. The pRSV-T plasmid was transfected into mouse hepatic cells to establish an SV40LT-immortalized mouse hepatic cell line. The SV40LT-immortalized mouse hepatic cells were identified and transfected with the pHBV1.3 plasmid. The levels of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) in the supernatant were determined by an electrochemiluminescence immunoassay at 24, 48, 72 and 96 h after transfection. The expressions of HBsAg and hepatitis B c antigen (HBcAg) in the cells were investigated by indirect immunofluorescence analysis. The presence of HBV DNA replication intermediates in the transfected cells and viral particles in the supernatant of the transfected cell cultures was monitored using the Southern hybridization assay and transmission electronic microscopy, respectively. The pRSV-T plasmid was used to immortalize mouse hepatocytes and an SV40LT-immortalized mouse hepatic cell line was successfully established. SV40LT-immortalized mouse hepatic cells have the same morphology and growth characteristics as primary mouse hepatic cells can be subcultured and produce albumin and cytokeratin-18 in vitro. Immortalized mouse hepatic cells did not show the characteristics of tumor cells, as alpha-fetoprotein levels were comparable (0.58 ± 0.37 vs 0.61 ± 0.31, P = 0.37). SV40LT-immortalized mouse hepatic cells were then transfected with the pHBV1.3 plasmid, and it was found that the HBV genome replicated in SV40LT-immortalized mouse hepatic cells. The levels of HBsAg and HBeAg continuously increased in the supernatant after the transfection of pHBV1.3, and began to decrease 72 h after transfection. The

  14. Study of runaway electrons using dosimetry of hard x-ray radiations in Damavand tokamak

    NASA Astrophysics Data System (ADS)

    Rasouli, C.; Pourshahab, B.; Hosseini Pooya, S. M.; Orouji, T.; Rasouli, H.

    2014-05-01

    In this work several studies have been conducted on hard x-ray emissions of Damavand tokamak based on radiation dosimetry using the Thermoluminescence method. The goal was to understand interactions of runaway electrons with plasma particles, vessel wall, and plasma facing components. Total of 354 GR-200 (LiF:Mg,Cu,P) thermoluminescence dosimeter (TLD) crystals have been placed on 118 points - three TLDs per point - to map hard x-ray radiation doses on the exterior of the vacuum vessel. Results show two distinctive levels of x-ray radiations doses on the exterior of the vessel. The low-dose area on which measured dose is about 0.5 mSv/shot. In the low-dose area there is no particular component inside the vessel. On the contrary, on high-dose area of the vessel, x-ray radiations dose exceeds 30 mSv/shot. The high-dose area coincides with the position of limiters, magnetic probe ducts, and vacuum vessel intersections. Among the high-dose areas, the highest level of dose is measured in the position of the limiter, which could be due to its direct contact with the plasma column and with runaway electrons. Direct collisions of runaway electrons with the vessel wall and plasma facing components make a major contribution for production of hard x-ray photons in Damavand tokamak.

  15. Study of runaway electrons using dosimetry of hard x-ray radiations in Damavand tokamak.

    PubMed

    Rasouli, C; Pourshahab, B; Hosseini Pooya, S M; Orouji, T; Rasouli, H

    2014-05-01

    In this work several studies have been conducted on hard x-ray emissions of Damavand tokamak based on radiation dosimetry using the Thermoluminescence method. The goal was to understand interactions of runaway electrons with plasma particles, vessel wall, and plasma facing components. Total of 354 GR-200 (LiF:Mg,Cu,P) thermoluminescence dosimeter (TLD) crystals have been placed on 118 points--three TLDs per point--to map hard x-ray radiation doses on the exterior of the vacuum vessel. Results show two distinctive levels of x-ray radiations doses on the exterior of the vessel. The low-dose area on which measured dose is about 0.5 mSv/shot. In the low-dose area there is no particular component inside the vessel. On the contrary, on high-dose area of the vessel, x-ray radiations dose exceeds 30 mSv/shot. The high-dose area coincides with the position of limiters, magnetic probe ducts, and vacuum vessel intersections. Among the high-dose areas, the highest level of dose is measured in the position of the limiter, which could be due to its direct contact with the plasma column and with runaway electrons. Direct collisions of runaway electrons with the vessel wall and plasma facing components make a major contribution for production of hard x-ray photons in Damavand tokamak.

  16. 23 CFR 1210.5 - Certification requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... requirements of 23 U.S.C. 161, and this part. (b) The certification shall contain: (1) A copy of the State zero..., that the State has enacted and is enforcing a conforming zero tolerance law. The certifying statement... a zero tolerance law that conforms to the requirements of 23 U.S.C. 161 and 23 CFR 1210.4(c). (c) An...

  17. 23 CFR 1210.5 - Certification requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requirements of 23 U.S.C. 161, and this part. (b) The certification shall contain: (1) A copy of the State zero..., that the State has enacted and is enforcing a conforming zero tolerance law. The certifying statement... a zero tolerance law that conforms to the requirements of 23 U.S.C. 161 and 23 CFR 1210.4(c). (c) An...

  18. 23 CFR 1210.5 - Certification requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requirements of 23 U.S.C. 161, and this part. (b) The certification shall contain: (1) A copy of the State zero..., that the State has enacted and is enforcing a conforming zero tolerance law. The certifying statement... a zero tolerance law that conforms to the requirements of 23 U.S.C. 161 and 23 CFR 1210.4(c). (c) An...

  19. 23 CFR 1210.5 - Certification requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requirements of 23 U.S.C. 161, and this part. (b) The certification shall contain: (1) A copy of the State zero..., that the State has enacted and is enforcing a conforming zero tolerance law. The certifying statement... a zero tolerance law that conforms to the requirements of 23 U.S.C. 161 and 23 CFR 1210.4(c). (c) An...

  20. X-40A Free Flight #5

    NASA Image and Video Library

    2001-05-08

    X-40A Free Flight #5. The unpowered X-40A, an 85 percent scale risk reduction version of the proposed X-37, proved the capability of an autonomous flight control and landing system in a series of glide flights at NASA's Dryden Flight Research Center in California. NASA's Marshall Space Flight Center in Huntsville, Alabama, manages the X-37 project. At Dryden, the X-40A underwent a series of ground and air tests to reduce possible risks to the larger X-37, including drop tests from a helicopter to check guidance and navigation systems planned for use in the X-37. The X-37 is designed to demonstrate technologies in the orbital and reentry environments for next-generation reusable launch vehicles that will increase both safety and reliability, while reducing launch costs from $10,000 per pound to $1,000 per pound.

  1. 22 CFR 23.5 - Claims for settlement by Department of State or General Accounting Office.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... General Accounting Office. 23.5 Section 23.5 Foreign Relations DEPARTMENT OF STATE FEES AND FUNDS FINANCE AND ACCOUNTING § 23.5 Claims for settlement by Department of State or General Accounting Office. Claims for settlement by the Department of State or by the General Accounting Office shall be submitted...

  2. Transformation and radiosensitivity of human diploid skin fibroblasts transfected with activated ras oncogene and SV40 T-antigen.

    PubMed

    Su, L N; Little, J B

    1992-08-01

    Three normal human diploid cell strains were transfected with an activated Ha-ras oncogene (EJ ras) or SV40 T-antigen. Multiple clones were examined for morphological alterations, growth requirements, ability to grow under anchorage independent conditions, immortality and tumorigenicity in nude mice. Clones expressing SV40 T-antigen alone or in combination with ras protein p21 were significantly radioresistant as compared with their parent cells or clones transfected with the neo gene only. This radioresistant phenotype persisted in post-crisis, immortalized cell lines. Cells transfected with EJ ras alone showed no morphological alterations nor significant changes in radiosensitivity. Cell clones expressing ras and/or SV40 T-antigen showed a reduced requirement for serum supplements, an increase in aneuploidy and chromosomal aberrations, and enhanced growth in soft agar as an early cellular response to SV40 T-antigen expression. The sequential order of transfection with SV40 T-antigen and ras influenced radio-sensitivity but not the induction of morphological changes. These data suggest that expression of the SV40 T-antigen but not activated Ha-ras plays an important role in the radiosensitivity of human diploid cells. The radioresistant phenotype in SV40 T transfected cells was not related to the enhanced level of genetic instability seen in pre-crisis and newly immortalized cells, nor to the process of immortalization itself.

  3. Repeated Administration of D-Amphetamine Induces Distinct Alterations in Behavior and Metabolite Levels in 129Sv and Bl6 Mouse Strains.

    PubMed

    Vanaveski, Taavi; Narvik, Jane; Innos, Jürgen; Philips, Mari-Anne; Ottas, Aigar; Plaas, Mario; Haring, Liina; Zilmer, Mihkel; Vasar, Eero

    2018-01-01

    The main goal of the study was to characterize the behavioral and metabolomic profiles of repeated administration (for 11 days) of d-amphetamine (AMPH, 3 mg/kg i. p.), indirect agonist of dopamine (DA), in widely used 129S6/SvEvTac (129Sv) and C57BL/6NTac (Bl6) mouse strains. Acute administration of AMPH (acute AMPH) induced significantly stronger motor stimulation in Bl6. However, repeated administration of AMPH (repeated AMPH) caused stronger motor sensitization in 129Sv compared acute AMPH. Body weight of 129Sv was reduced after repeated saline and AMPH, whereas no change occurred in Bl6. In the metabolomic study, acute AMPH induced an elevation of isoleucine and leucine, branched chain amino acids (BCAA), whereas the level of hexoses was reduced in Bl6. Both BCAAs and hexoses remained on level of acute AMPH after repeated AMPH in Bl6. Three biogenic amines [asymmetric dimethylarginine (ADMA), alpha-aminoadipic acid (alpha-AAA), kynurenine] were significantly reduced after repeated AMPH. Acute AMPH caused in 129Sv a significant reduction of valine, lysophosphatidylcholines (lysoPC a C16:0, lysoPC a C18:2, lysoPC a C20:4), phosphatidylcholine (PC) diacyls (PC aa C34:2, PC aa C36:2, PC aa C36:3, PC aa C36:4) and alkyl-acyls (PC ae C38:4, PC ae C40:4). However, repeated AMPH increased the levels of valine and isoleucine, long-chain acylcarnitines (C14, C14:1-OH, C16, C18:1), PC diacyls (PC aa C38:4, PC aa C38:6, PC aa C42:6), PC acyl-alkyls (PC ae C38:4, PC ae C40:4, PC ae C40:5, PC ae C40:6, PC ae C42:1, PC ae C42:3) and sphingolipids [SM(OH)C22:1, SM C24:0] compared to acute AMPH in 129Sv. Hexoses and kynurenine were reduced after repeated AMPH compared to saline in 129Sv. The established changes probably reflect a shift in energy metabolism toward lipid molecules in 129Sv because of reduced level of hexoses. Pooled data from both strains showed that the elevation of isoleucine and leucine was a prominent biomarker of AMPH-induced behavioral sensitization

  4. Water vapour masers in long-period variable stars. I. RX Bootis and SV Pegasi

    NASA Astrophysics Data System (ADS)

    Winnberg, A.; Engels, D.; Brand, J.; Baldacci, L.; Walmsley, C. M.

    2008-05-01

    Context: Water vapour maser emission from late-type stars characterises them as asymptotic-giant-branch stars with oxygen-rich chemistry that are losing mass at a substantial rate. Further conclusions on the properties of the stars, however, are hampered by the strong variability of the emission. Aims: We wish to understand the reasons for the strong variability of H2O masers in circumstellar shells of late-type stars. In this paper we study RX Bootis and SV Pegasi as representatives of semiregular variable stars (SRVs). Methods: We monitored RX Boo and SV Peg in the 22-GHz maser line of water vapour with single-dish telescopes. The monitoring period covered two decades for RX Boo (1987-2007) and 12 years for SV Peg (1990-1995, 2000-2007). In addition, maps were obtained of RX Boo with the Very Large Array over several years. Results: We find that most of the emission in the circumstellar shell of RX Boo is located in an incomplete ring with an inner radius of 91 mas (15 AU). A velocity gradient is found in a NW-SE direction. The maser region can be modelled as a shell with a thickness of 22 AU, which is only partially filled. The gas crossing time is 16.5 years. The ring-like structure and the velocity gradient remained stable for at least 11 years, while the maser line profiles varied strongly. This suggests that the spatial asymmetry is not accidental, so that either the mass loss process or the maser excitation conditions in RX Boo are not spherically symmetric. The strong variability of the maser spectral features is mainly due to incoherent intensity fluctuations of maser emission spots, which have lifetimes of the order of 1 year. We found no correlation between the optical and the maser variability in either star. The variability properties of the SV Peg masers do not differ substantially from those of RX Boo. There were fewer spectral features present, and the range of variations was narrower. The maser was active on the >10-Jy level only 1990-1992 and

  5. Gene expression promoted by the SV40 DNA targeting sequence and the hypoxia-responsive element under normoxia and hypoxia.

    PubMed

    Sacramento, C B; Moraes, J Z; Denapolis, P M A; Han, S W

    2010-08-01

    The main objective of the present study was to find suitable DNA-targeting sequences (DTS) for the construction of plasmid vectors to be used to treat ischemic diseases. The well-known Simian virus 40 nuclear DTS (SV40-DTS) and hypoxia-responsive element (HRE) sequences were used to construct plasmid vectors to express the human vascular endothelial growth factor gene (hVEGF). The rate of plasmid nuclear transport and consequent gene expression under normoxia (20% O2) and hypoxia (less than 5% O2) were determined. Plasmids containing the SV40-DTS or HRE sequences were constructed and used to transfect the A293T cell line (a human embryonic kidney cell line) in vitro and mouse skeletal muscle cells in vivo. Plasmid transport to the nucleus was monitored by real-time PCR, and the expression level of the hVEGF gene was measured by ELISA. The in vitro nuclear transport efficiency of the SV40-DTS plasmid was about 50% lower under hypoxia, while the HRE plasmid was about 50% higher under hypoxia. Quantitation of reporter gene expression in vitro and in vivo, under hypoxia and normoxia, confirmed that the SV40-DTS plasmid functioned better under normoxia, while the HRE plasmid was superior under hypoxia. These results indicate that the efficiency of gene expression by plasmids containing DNA binding sequences is affected by the concentration of oxygen in the medium.

  6. Programs for radioimmunoassays and radioreceptorassays using SPSS-X.

    PubMed

    Amador, A G; Hodges, S L

    1989-01-01

    Two sets of programs written using SPSS-X, and designed for the analysis of data obtained by radioimmunoassay or radioreceptorassay are presented. The main advantages of the programs are that they are easily edited, their commands are simple and logical, and they can be used for any type of radioimmunoassay or radioreceptorassay, respectively. The programs for radioimmunoassay can also be used for fluorescenceimmunoassay and luminescenceimmunoassay.

  7. Crystal structure, oxidation state and magnetism of Sr{sub x}La{sub 2−x}Cu{sub 0.5}Ru{sub 0.5}O{sub 4} (x=1, 1.5)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lü, Minfeng, E-mail: m.f.lv@ciac.jl.cn; Deng, Xiaolong; Waerenborgh, João C.

    2014-03-15

    Sr{sub x}La{sub 2−x}Cu{sub 0.5}Ru{sub 0.5}O{sub 4} (x=1, 1.5) oxides with K{sub 2}NiF{sub 4}-type structure were prepared by solid state reaction and characterized by powder X-ray diffraction, X-ray photoelectron spectroscopy, magnetic and electrical resistivity measurements. The SrLaCu{sub 0.5}Ru{sub 0.5}O{sub 4} phase was obtained for the first time with a negligible amount of impurities. The octahedral Cu/RuO{sub 6} units are more elongated in SrLaCu{sub 0.5}Ru{sub 0.5}O{sub 4} than in Sr{sub 1.5}La{sub 0.5}Cu{sub 0.5}Ru{sub 0.5}O{sub 4} indicating a greater extent of static Jahn–Teller distortion. XPS suggests that mixed ion pairs Ru{sup 5+}/Ru{sup 4+}↔Cu{sup +}/Cu{sup 2+} are present in SrLaCu{sub 0.5}Ru{sub 0.5}O{sub 4}, whilemore » Ru remains as Ru{sup 5+} and Cu as Cu{sup 2+} in Sr{sub 1.5}La{sub 0.5}Cu{sub 0.5}Ru{sub 0.5}O{sub 4}. Both samples show spin-glass behavior, which can be explained by competition between ferromagnetic and antiferromagnetic superexchange interactions. The negative Weiss temperature estimated for SrLaCu{sub 0.5}Ru{sub 0.5}O{sub 4}, −318 K, is significantly lower than −11.5 K deduced for Sr{sub 1.5}La{sub 0.5}Cu{sub 0.5}Ru{sub 0.5}O{sub 4} which may be related to the higher static Jahn–Teller distortion in the former oxide. -- Graphical abstract: SrLaCu{sub 0.5}Ru{sub 0.5}O{sub 4} with K{sub 2}NiF{sub 4}-type structure show a larger static Jahn–Teller distortion than Sr{sub 1.5}La{sub 0.5}Cu{sub 0.5}Ru{sub 0.5}O{sub 4}, which may be related to stronger antiferromagnetic superexchange interactions. Highlights: • SrLaCu{sub 0.5}Ru{sub 0.5}O{sub 4} (I) larger Jahn–Teller (J–T) distortion than Sr{sub 1.5}La{sub 0.5}Cu{sub 0.5}Ru{sub 0.5}O{sub 4} (II). • Octahedral Cu/RuO{sub 6} units are more elongated in I than in II. • Mixed ion pairs Ru{sup 5+}/Ru{sup 4+}↔Cu{sup +}/Cu{sup 2+} are present in I, while Ru remains as Ru{sup 5+} and Cu as Cu{sup 2+} in II. • Negative Weiss temperature of I significantly lower

  8. A Taxometric Analysis of the Psychopathy Checklist: Screening Version (PCL:SV): Further Evidence of Dimensionality

    ERIC Educational Resources Information Center

    Walters, Glenn D.; Gray, Nicola S.; Jackson, Rebecca L.; Sewell, Kenneth W.; Rogers, Richard; Taylor, John; Snowden, Robert J.

    2007-01-01

    A taxometric analysis of the Psychopathy Checklist: Screening Version (PCL:SV; S. D. Hart, D. N. Cox, & R. D. Hare, 1995) was performed on a group of 2,250 male and female forensic/psychiatric patients and jail/prison inmates. The 4 PCL:SV facet scores (Interpersonal, Affective, Impulsive Lifestyle, Antisocial Behavior) served as indicators in…

  9. 23 CFR Appendix B to Part 1200 - HIGHWAY SAFETY PROGRAM COST SUMMARY (HS-217)

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false HIGHWAY SAFETY PROGRAM COST SUMMARY (HS-217) B APPENDIX B TO PART 1200 Highways NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION AND FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PROCEDURES FOR STATE HIGHWAY SAFETY PROGRAMS UNIFORM PROCEDURES FOR...

  10. 23 CFR Appendix B to Part 1200 - Highway Safety Program Cost Summary (HS-217)

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Highway Safety Program Cost Summary (HS-217) B Appendix B to Part 1200 Highways NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION AND FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION PROCEDURES FOR STATE HIGHWAY SAFETY PROGRAMS UNIFORM PROCEDURES FOR...

  11. X-wing noise data acquisition program

    NASA Technical Reports Server (NTRS)

    Healy, G. J.

    1983-01-01

    The X-wing circulation controlled rotor system model was tested for hover performance. During these performance tests, noise data from 12 microphones was recorded on magnetic tape for subsequent data reduction. The rotor system was operated at 4 tip speeds ranging from 529 to 650 ft./sec. (404 to 497 rpm), collective angles of attack fro 0 deg to 8.5 deg (maximum), and blade pressure ratios from 1.0 (no blowing) to a maximum of 2.1. The 12 microphones included 11 in the far field, and one in the transmission area. Following completion of the rotor and subsystem noise measurements, sound field calibration measurements were made of both the rotor 'bowl' and the loudspeaker system used in the 'bowl' calibration measurements. The location of 10 far field microphones was measured by a surveyor. Additionally, detailed tape logs were prepared for the six reels of tape used for the program.

  12. 23 CFR 1345.5 - Requirements for a grant.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... must adopt and implement effective programs to reduce highway deaths and injuries resulting from... strategy that the State intends to use to deliver each message to its target audience. (5) To demonstrate...) Child passenger protection education program. (1) A State must provide an effective system for educating...

  13. 23 CFR 1345.5 - Requirements for a grant.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... must adopt and implement effective programs to reduce highway deaths and injuries resulting from... strategy that the State intends to use to deliver each message to its target audience. (5) To demonstrate...) Child passenger protection education program. (1) A State must provide an effective system for educating...

  14. Electrochemical properties of LaNi{sub 5{minus}x}Ge{sub x} alloys in Ni-MH batteries

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Witham, C.; Hightower, A.; Fultz, B.

    1997-11-01

    Electrochemical studies were performed on LaNi{sub 5{minus}x}Ge{sub x} metal hydride alloys with 0 {le} x {le} 0.5. The authors carried out single-electrode studies to understand the effects of the Ge substituent on the hydrogen absorption characteristics, the electrochemical capacity, and the electrochemical kinetics of hydrogen absorption and desorption. The electrochemical characteristics of the Ge-substituted alloys are compared to those of the Sn-substituted alloys reported earlier. LaNi{sub 5{minus}x}Ge{sub x} alloys show compositional trends similar to LaNi{sub 5{minus}x}Sn{sub x} alloys, but unlike the Sn-substituted alloys, Ge-substituted alloys continue to exhibit facile kinetics for hydrogen absorption/desorption at high solute concentrations. Cycle lives ofmore » LaNi{sub 5{minus}x}Ge{sub x} electrodes were measured in 300 mAh laboratory test cells and were found to be superior to the Sn-substituted LaNi{sub 5} and comparable to a Mm(Ni, Co, Mn, Al){sub 5} alloy. The optimum Ge content for LaNi{sub 5{minus}x}Ge{sub x} metal hydride alloys in alkaline rechargeable cells is in the range 0.4 {le} x {le} 0.5.« less

  15. Investigation of sodium insertion–extraction in olivine Na x FePO 4 (0 ≤ x ≤ 1) using first-principles calculations

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Saracibar, A.; Carrasco, J.; Saurel, D.

    Olivine NaFePO4 has recently attracted the attention of the scientific community as a promising cathode material for Na-ion batteries. In this work we combine density functional theory (DFT) calculations and high resolution synchrotron X-ray diffraction (HRXRD) experiments to study the phase stability of NaxFePO4 along the whole range of sodium compositions (0 ≤ x ≤ 1). DFT calculations reveal the existence of two intermediate structures governing the phase stability at x = 2/3 and x = 5/6. This is in contrast to isostructural LiFePO4, which is a broadly used cathode in Li-ion batteries. Na2/3FePO4 and Na5/6FePO4 ground states both alignmore » vacancies diagonally within the ab plane, coupled to a Fe2+/Fe3+ alignment. HRXRD data for NaxFePO4 (2/3 < x < 1) materials show common superstructure reflections up to x = 5/6 within the studied compositions. The computed intercalation voltage profile shows a voltage difference of 0.16 V between NaFePO4 and Na2/3FePO4 in agreement with the voltage discontinuity observed experimentally during electrochemical insertion.« less

  16. 23 CFR 1.5 - Information furnished by State highway departments.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Information furnished by State highway departments. 1.5... ADMINISTRATION GENERAL § 1.5 Information furnished by State highway departments. At the request of the Administrator the State highway department shall furnish to him such information as the Administrator shall deem...

  17. 1. 8' x 10' enlargement from 4' x 5' negative ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. 8' x 10' enlargement from 4' x 5' negative Kevin Kriesel-Coons, Photographer, November 13, 1990 EXTERIOR OF HYDRO PLANT, SHOWING CURRENT STATE OF DISREPAIR. VIEW FROM WALKWAY OVER TAILRACE OF CROSSCUT CANAL TO THE LARGER, ORIGINAL CROSSCUT HYDRO PLANT. - Crosscut Steam Plant, Ancillary Hydro Unit, North side Salt River near Mill Avenue & Washington Street, Tempe, Maricopa County, AZ

  18. Relevance between SV and components based on water quality inspection by gas plumes

    NASA Astrophysics Data System (ADS)

    Nakanishi, A.; Aoyama, C.; Fukuoka, H.; Tajima, H.; Kumagai, H.; Takahashi, A.

    2017-12-01

    Gas and hydrate seeping from the seafloor into ocean water can be monitored on board, as images on echogram (acoustic equipment display inboard) by utilizing acoustic measurement equipment such as multi-beam sonars. Colors and shades of these images displayed on the monitor vary depending on the acoustic impedance. Backscattering strength (hereinafter referred as SV) depends on the type and density of plume components. Therefore, plume components should not be determined only by examining volume scattering density. By standardizing the relevance between gas plume SV and the components, types of plume components can be presumed just by calculating plume SV based on multi-beam data.Data from the following explorations will be utilized to perform the analysis of metal sensor, CTD measurement, and sampling. July, 2017 KAIYO-MARU2 (KAIYO ENGINEERING CO., LTD) @ Sea of Japan July, 2017 SIHNYO MARU (Tokyo University of Marine Science and Technology) @ Sea of Japan. And Chemical data obtained through YK16-07 cruise is also to be discussed.

  19. Ion mobility and conductivity in the M{sub 0.5–x}Pb{sub x}Bi{sub 0.5}F{sub 2+x} (M=K, Rb) solid solutions with fluorite structure

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kavun, V. Ya., E-mail: kavun@ich.dvo.ru; Uvarov, N.F.; Slobodyuk, A.B.

    Ionic mobility and conductivity in the K{sub 0.5–x}Pb{sub x}Bi{sub 0.5}F{sub 2+x} and Rb{sub 0.5–x}Pb{sub x}Bi{sub 0.5}F{sub 2+x} (x=0.05, 0.09) solid solutions with the fluorite structure have been investigated using the methods of {sup 19}F NMR, X-ray diffraction and impedance spectroscopy. Types of ionic motions in the fluoride sublattice of solid solutions have been established and temperature ranges of their realization have been determined (150–450 K). Diffusion of fluoride ions is a dominating type of ionic motions in the fluoride sublattice of solid solutions under study above 350 K. Due to high ionic conductivity, above 10{sup –3} S/cm at 450 K,more » these solid solutions can be used as solid electrolytes in various electrochemical devices and systems. - Graphical abstract: Temperature dependence of the concentration of mobile (2, 4) and immobile (1, 3) F ions in the K{sub 0.5–x}Pb{sub x}Bi{sub 0.5}F{sub 2+x} solid solutions. - Highlights: • Studied the ion mobility, conductivity in M{sub 0.5–x}Pb{sub x}Bi{sub 0.5}F{sub 2+x} solid solutions (M=K, Rb). • An analysis of {sup 19}F NMR spectra made it possible to identify types of ion mobility. • The main type of ion motion above 300 K in solid solutions is a diffusion of ions F{sup –}. • The ionic conductivity of the solid solutions studied more than 10{sup –3} S/cm at 450 K.« less

  20. Relationship between X(5) models and the interacting boson model

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Barea, Jose; Departamento de Fisica Atomica, Molecular y Nuclear, Facultad de Fisica, Universidad de Sevilla, Apartado 1065, E-41080 Sevilla; Arias, Jose M.

    2010-08-15

    The connections between the X(5) models [the original X(5) using an infinite square well, X(5)-{beta}{sup 8}, X(5)-{beta}{sup 6}, X(5)-{beta}{sup 4}, and X(5)-{beta}{sup 2}], based on particular solutions of the geometrical Bohr Hamiltonian with harmonic potential in the {gamma} degree of freedom, and the interacting boson model (IBM) are explored. This work is the natural extension of the work presented in Garcia-Ramos and Arias, Phys. Rev. C 77, 054307 (2008) for the E(5) models. For that purpose, a quite general one- and two-body IBM Hamiltonian is used and a numerical fit to the different X(5) model energies is performed; then themore » obtained wave functions are used to calculate B(E2) transition rates. It is shown that within the IBM one can reproduce well the results for energies and B(E2) transition rates obtained with all these X(5) models, although the agreement is not so impressive as for the E(5) models. From the fitted IBM parameters the corresponding energy surface can be extracted and, surprisingly, only the X(5) case corresponds in the moderately large N limit to an energy surface very close to the one expected for a critical point, whereas the rest of models are situated a little further away.« less

  1. Assessing risk for violence among male and female civil psychiatric patients: the HCR-20, PCL:SV, and VSC.

    PubMed

    Nicholls, Tonia L; Ogloff, James R P; Douglas, Kevin S

    2004-01-01

    This study evaluated the predictive validity of violence risk assessments conducted using the HCR-20, the Psychopathy Checklist: Screening Version (PCL:SV), and by the Violence Screening Checklist (VSC) in a sample of 268 involuntarily hospitalized male and female psychiatric patients. Information pertaining to violence and crime was coded from medical charts and correctional records. The HCR-20/PCL:SV evidenced modest non-significant associations in postdictive assessments of inpatient violence among men. Moderate to strong significant associations were found between the HCR-20/PCL:SV and inpatient violence among women. Pseudo-prospective assessments using the HCR-20 and PCL:SV resulted in moderate to large relationships with violence and crime in men and women following community discharge. It is concluded that the VSC is a promising tool for assessing acute inpatient violence risk with men. Findings offer preliminary validation of the predictive validity of the HCR-20 and PCL:SV with female civil psychiatric patients. Copyright 2004 John Wiley & Sons, Ltd.

  2. Atrial natriuretic peptide provokes a dramatic increase in cyclic GMP formation and markedly inhibits muscarinic-stimulated Ca2+ mobilisation in SV-40 transformed cat iris sphincter smooth muscle (SV-CISM-2) cells.

    PubMed

    Ding, K H; Ali, N; Abdel-Latif, A A

    1999-02-01

    We investigated the effects of cGMP-elevating agents, including atrial natriuretic peptide (ANP), C-type natriuretic peptide (CNP) and sodium nitroprusside (SNP), on cGMP accumulation and on carbachol (CCh)-stimulated intracellular calcium ([Ca2+]i) mobilisation in SV-40 transformed cat iris sphincter smooth muscle (SV-CISM-2) cells and in primary cultured cat iris sphincter smooth muscle (CISM) cells. The stimulatory effects of the natriuretic peptides on cGMP production correlated well with their inhibitory effects on CCh-induced [Ca+1]i mobilisation, and these effects were significantly more pronounced in the SV-CISM-2 cells than in the CISM cells. Thus, ANP (1 microM) increased cGMP production in the SV-CISM-2 cells and CISM cells by 487- and 1.7-fold, respectively, and inhibited CCh-induced [Ca2+]i mobilisation by 95 and 3%, respectively. In the SV-CISM-2 cells, ANP and CNP dose dependently inhibited CCh-induced [Ca2+]i mobilisation with IC50 values of 156 and 412 nM, respectively, and dose dependently stimulated cGMP formation with EC50 values of 24 and 88 nM, respectively, suggesting that the inhibitory actions of the peptides are mediated through cGMP. Both ANP and CNP stimulated cGMP accumulation in a time-dependent manner. The potency of the cGMP-elevating agents were in the following order: ANP>CNP>SNP; these agents had no effect on cAMP accumulation. The inhibitory effects of the natriuretic peptides were mimicked by 8-Br-cGMP, a selective activator of cGMP-dependent protein kinase. LY83583, a soluble guanylyl cyclase inhibitor, significantly inhibited SNP-induced cGMP formation but had no effect on those of ANP and CNP. The basal activities of the guanylyl cyclase and the dissociation constant (Kd) and total receptor density (Bmax) values of the natriuretic peptide receptor for [125I]ANP binding were not significantly different between the two cell types. The cGMP system, as with the cAMP system, has a major inhibitory influence on the muscarinic

  3. SV40 host-substituted variants: a new look at the monkey DNA inserts and recombinant junctions.

    PubMed

    Singer, Maxine; Winocour, Ernest

    2011-04-10

    The available monkey genomic data banks were examined in order to determine the chromosomal locations of the host DNA inserts in 8 host-substituted SV40 variant DNAs. Five of the 8 variants contained more than one linked monkey DNA insert per tandem repeat unit and in all cases but one, the 19 monkey DNA inserts in the 8 variants mapped to different locations in the monkey genome. The 50 parental DNAs (32 monkey and 18 SV40 DNA segments) which spanned the crossover and flanking regions that participated in monkey/monkey and monkey/SV40 recombinations were characterized by substantial levels of microhomology of up to 8 nucleotides in length; the parental DNAs also exhibited direct and inverted repeats at or adjacent to the crossover sequences. We discuss how the host-substituted SV40 variants arose and the nature of the recombination mechanisms involved. Copyright © 2011 Elsevier Inc. All rights reserved.

  4. Differential effects of simple repeating DNA sequences on gene expression from the SV40 early promoter.

    PubMed

    Amirhaeri, S; Wohlrab, F; Wells, R D

    1995-02-17

    The influence of simple repeat sequences, cloned into different positions relative to the SV40 early promoter/enhancer, on the transient expression of the chloramphenicol acetyltransferase (CAT) gene was investigated. Insertion of (G)29.(C)29 in either orientation into the 5'-untranslated region of the CAT gene reduced expression in CV-1 cells 50-100 fold when compared with controls with random sequence inserts. Analysis of CAT-specific mRNA levels demonstrated that the effect was due to a reduction of CAT mRNA production rather than to posttranscriptional events. In contrast, insertion of the same insert in either orientation upstream of the promoter-enhancer or downstream of the gene stimulated gene expression 2-3-fold. These effects could be reversed by cotransfection of a competitor plasmid carrying (G)25.(C)25 sequences. The results suggest that a G.C-binding transcription factor modulates gene expression in this system and that promoter strength can be regulated by providing protein-binding sites in trans. Although constructs containing longer tracts of alternating (C-G), (T-G), or (A-T) sequences inhibited CAT expression when inserted in the 5'-untranslated region of the CAT gene, the amount of CAT mRNA was unaffected. Hence, these inhibitions must be due to posttranscriptional events, presumably at the level of translation. These effects of microsatellite sequences on gene expression are discussed with respect to recent data on related simple repeat sequences which cause several human genetic diseases.

  5. Automatic evaluation of the Valsalva sinuses from cine-MRI

    NASA Astrophysics Data System (ADS)

    Blanchard, Cédric; Sliwa, Tadeusz; Lalande, Alain; Mohan, Pauliah; Bouchot, Olivier; Voisin, Yvon

    2011-03-01

    MRI appears to be particularly attractive for the study of the Sinuses of Valsalva (SV), however there is no global consensus on their suitable measurements. In this paper, we propose a new method, based on the mathematical morphology and combining a numerical geodesic reconstruction with an area estimation, to automatically evaluate the SV from a cine-MRI in a cross-sectional orientation. It consists in the extraction of the shape of the SV, the detection of relevant points (commissures, cusps and the centre of the SV), the measurement of relevant distances and in a classification of the valve as bicuspid or tricuspid by a metric evaluation of the SV. Our method was tested on 23 patient examinations and radii calculations were compared with a manual measurement. The classification of the valve as tricuspid or bicuspid was correct for all the cases. Moreover, there are an excellent correlation and an excellent concordance between manual and automatic measurements for images at diastolic phase (r= 0.97; y = x - 0.02; p=NS; mean of differences = -0.1 mm; standard deviation of differences = 2.3 mm) and at systolic phase (r= 0.96; y = 0.97 x + 0.80; p=NS ; mean of differences = -0.1 mm; standard deviation of differences = 2.4 mm). The cross-sectional orientation of the image acquisition plane conjugated with our automatic method provides a reliable morphometric evaluation of the SV, based on the automatic location of the centre of the SV, the commissure and the cusp positions. Measurements of distances between relevant points allow a precise evaluation of the SV.

  6. X-38 Program Status/Overview

    NASA Technical Reports Server (NTRS)

    Anderson, Brian L.

    2001-01-01

    The X-38 Project consists of a series of experimental vehicles designed to provide the technical "blueprint" for the International Space Station's (ISS) Crew Return Vehicle (CRV). There are three atmospheric vehicles and one space flight vehicle in the program. Each vehicle is designed as a technical stepping stone for the next vehicle, with each new vehicle being more complex and advanced than it's predecessor. The X-38 project began in 1995 at the Johnson Space Center (JSC) in Houston, Texas at the direction of the NASA administrator. From the beginning, the project has had the CRY design validation as its ultimate goal. The CRY has three basic missions that drive the design that must be proven during the course of the X-38 Project: a) Emergency return of an ill or injured crew member. b) Emergency return of an entire ISS crew due to the inability of ISS to sustain life c) Planned return of an entire ISS crew due to the inability to re-supply the ISS or return the crew. The X-38 project must provide the blueprint for a vehicle that provides the capability for human return from space for all three of these design missions.

  7. 0.5-4 Å X-RAY BRIGHTENINGS IN THE MAGNETOSPHERE OBSERVED BY THE GEOSTATIONARY OPERATIONAL ENVIRONMENTAL SATELLITES

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Yamamoto, Tetsuya T.; Miyoshi, Y., E-mail: tyamamot@stelab.nagoya-u.ac.jp

    We found 217 X-ray brightening events in Earth's magnetosphere. These events occur in the high-energy band (0.5-4 Å) of the Geostationary Operational Environmental Satellite (GOES) X-ray light curves, although GOES X-ray light curves are frequently used as indices of solar flare magnitudes. We found that (1) brightening events are absent in the low-energy band (1-8 Å), unlike those associated with solar flares; and (2) the peak fluxes, durations, and onset times of these events depend on the magnetic local time (MLT). The events were detected in 2006, 2010, and 2011 at around 19-10 MLT, that is, from night to morning.more » They typically lasted for 2-3 hr. Their peak fluxes are less than 3 × 10{sup –8} W m{sup –2} in the 0.5-4 Å band and are maximized around 0-5 MLT. From these MLT dependencies, we constructed an MLT time profile of X-ray brightening events. Because 0.5-4 and 1-8 Å fluxes were observed and had the same order of magnitude when GOES 14 passed through Earth's shadow, we expected that X-ray brightening events in the 1-8 Å band are obscured by high-background X-ray fluxes coming from the Sun. We also found coincidence between X-ray brightening events and aurora substorms. In the majority of our events, the minimum geomagnetic field values (AL index) are below –400 nT. From these results and consideration of the GOES satellite orbit, we expect that these X-ray brightening events occur in the magnetosphere. We cannot, however, clarify the radiative process of the observed X-ray brightening events.« less

  8. 5. INTERIOR, TWO 5' X 6' HIGH PRESSURE EMERGENCY GATES, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. INTERIOR, TWO 5' X 6' HIGH PRESSURE EMERGENCY GATES, FOR BUREAU OF RECLAIMATION, WITH HYDRAULIC HOISTS, INSIDE OF MACHINE SHOP (CA 1930S). - Hardie-Tynes Manufacturing Company, Workshop, 800 Twenty-eighth Street North, Birmingham, Jefferson County, AL

  9. Understanding peptide competitive inhibition of botulinum neurotoxin A binding to SV2 protein via molecular dynamics simulations.

    PubMed

    Chang, Shan; He, Hong-Qiu; Shen, Lin; Wan, Hua

    2015-10-01

    Botulinum neurotoxins (BoNTs) are known as the most toxic natural substances. Synaptic vesicle protein 2 (SV2) has been proposed to be a protein receptor for BoNT/A. Recently, two short peptides (BoNT/A-A2 and SV2C-A3) were designed to inhibit complex formation between the BoNT/A receptor-binding domain (BoNT/A-RBD) and the synaptic vesicle protein 2C luminal domain (SV2C-LD). In this article, the two peptide complex systems are studied by molecular dynamics (MD) simulations. The structural stability analysis indicates that BoNT/A-A2 system is more stable than SV2C-A3 system. The conformational analysis implies that the β-sheet in BoNT/A-A2 system maintains its secondary structure but the two β-strands in SV2C-A3 system have remarkable conformational changes. Based on the calculation of hydrogen bonds, hydrophobic interactions and cation-π interactions, it is found that the internal hydrogen bonds play crucial roles in the structural stability of the peptides. Because of the stable secondary structure, the β-sheet in BoNT/A-A2 system establishes effective interactions at the interface and inhibits BoNT/A-RBD binding to SV2C-LD. In contrast, without other β-strands forming internal hydrogen bonds, the two isolated β-strands in SV2C-A3 system become the random coil. This conformational change breaks important hydrogen bonds and weakens cation-π interaction in the interface, so the complex formation is only partially inhibited by the two β-strands. These results are consistent with experimental studies and may be helpful in understanding the inhibition mechanisms of peptide inhibitors. © 2015 Wiley Periodicals, Inc.

  10. Session: Program Review X Wrap-Up

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    None

    1992-01-01

    This wrap-up session at the Geothermal Energy Program Review X: Geothermal Energy and the Utility Market consisted of Closing Remarks by Roland R. Kessler and six NGA Industry Critique Panel presentations: ''Summary of Comments on DOE-Industry Cooperation by Geothermal Industry Panel'' by James B. Koenig, GeothermEx, Inc.; ''NGA Industry Critique of the Exploration Component'' by Joe L. Iovenitti, Weiss Associates; ''Critique of Drilling Research'' by Jerry Hamblin, UNOCAL Geothermal; ''Critique Panel Comments on Reservoir Engineering, DOE Geothermal Technology Development'' by Dennis Kaspereit, California Energy Company, Inc.; ''DOE Geothermal Program Review - Critique on Production'' by Douglas B. Jung, Two-Phase Engineeringmore » and Research; ''Comments on the DOE Hydrothermal Energy Conversion R&D Program'' by David L. Mendive, Geothermal Development Associates.« less

  11. Magnetisation studies of phase co-existence in Gd{sub 1-x}Ca{sub x}BaCo{sub 2}O{sub 5.5}

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Thirumurugan, N.; Bharathi, A., E-mail: bharathi@igcar.gov.in; Arulraj, A.

    2012-04-15

    Highlights: Black-Right-Pointing-Pointer The series Gd{sub 1-x}Ca{sub x}BaCo{sub 2}O{sub 5.5} was synthesised by solid state reaction. Black-Right-Pointing-Pointer Magnetisation studies were carried out in the 4-300 K temperature range in magnetic fields upto 16 Tesla. Black-Right-Pointing-Pointer Results were used to formulate the T versus Ca fraction, phase diagram. Black-Right-Pointing-Pointer Evidence for Magnet-electronic phase separation is shown for the first time in the compound. -- Abstract: Magnetic properties of hole doped, oxygen deficient double perovskite compounds, Gd{sub 1-x}Ca{sub x}BaCo{sub 2}O{sub 5.5}, have been investigated. Ferromagnetic transition temperatures increase and the anti-ferromagnetic transition temperatures decrease with Ca substitution leading to stabilisation of ferromagnetisim formore » x {>=} 0.05. A detailed study of the ferromagnetic phase indicates the presence of double hysterisis loops for Ca fractions, 0.05 {<=} x {<=} 0.2 in the 50-200 K temperature range, suggestive of the co-existence of two ferromagnetic phases with different co-ercivities. Based on the magnetisation and transport measurements a phase diagram is proposed for Ca doped GdBaCo{sub 2}O{sub 5.5}.« less

  12. A Highly Conserved Salt Bridge Stabilizes the Kinked Conformation of β2,3-Sheet Essential for Channel Function of P2X4 Receptors.

    PubMed

    Zhao, Wen-Shan; Sun, Meng-Yang; Sun, Liang-Fei; Liu, Yan; Yang, Yang; Huang, Li-Dong; Fan, Ying-Zhe; Cheng, Xiao-Yang; Cao, Peng; Hu, You-Min; Li, Lingyong; Tian, Yun; Wang, Rui; Yu, Ye

    2016-04-08

    Significant progress has been made in understanding the roles of crucial residues/motifs in the channel function of P2X receptors during the pre-structure era. The recent structural determination of P2X receptors allows us to reevaluate the role of those residues/motifs. Residues Arg-309 and Asp-85 (rat P2X4 numbering) are highly conserved throughout the P2X family and were involved in loss-of-function polymorphism in human P2X receptors. Previous studies proposed that they participated in direct ATP binding. However, the crystal structure of P2X demonstrated that those two residues form an intersubunit salt bridge located far away from the ATP-binding site. Therefore, it is necessary to reevaluate the role of this salt bridge in P2X receptors. Here, we suggest the crucial role of this structural element both in protein stability and in channel gating rather than direct ATP interaction and channel assembly. Combining mutagenesis, charge swap, and disulfide cross-linking, we revealed the stringent requirement of this salt bridge in normal P2X4 channel function. This salt bridge may contribute to stabilizing the bending conformation of the β2,3-sheet that is structurally coupled with this salt bridge and the α2-helix. Strongly kinked β2,3 is essential for domain-domain interactions between head domain, dorsal fin domain, right flipper domain, and loop β7,8 in P2X4 receptors. Disulfide cross-linking with directions opposing or along the bending angle of the β2,3-sheet toward the α2-helix led to loss-of-function and gain-of-function of P2X4 receptors, respectively. Further insertion of amino acids with bulky side chains into the linker between the β2,3-sheet or the conformational change of the α2-helix, interfering with the kinked conformation of β2,3, led to loss-of-function of P2X4 receptors. All these findings provided new insights in understanding the contribution of the salt bridge between Asp-85 and Arg-309 and its structurally coupled β2,3-sheet to the

  13. PSTha5a23, a candidate effector from the obligate biotrophic pathogen Puccinia striiformis f. sp. tritici, is involved in plant defense suppression and rust pathogenicity.

    PubMed

    Cheng, Yulin; Wu, Kuan; Yao, Juanni; Li, Shumin; Wang, Xiaojie; Huang, Lili; Kang, Zhensheng

    2017-05-01

    During the infection of host plants, pathogens can deliver virulence-associated 'effector' proteins to promote plant susceptibility. However, little is known about effector function in the obligate biotrophic pathogen Puccinia striiformis f. sp. tritici (Pst) that is an important fungal pathogen in wheat production worldwide. Here, they report their findings on an in planta highly induced candidate effector from Pst, PSTha5a23. The PSTha5a23 gene is unique to Pst and shows a low level of intra-species polymorphism. It has a functional N-terminal signal peptide and is translocated to the host cytoplasm after infection. Overexpression of PSTha5a23 in Nicotiana benthamiana was found to suppress the programmed cell death triggered by BAX, PAMP-INF1 and two resistance-related mitogen-activated protein kinases (MKK1 and NPK1). Overexpression of PSTha5a23 in wheat also suppressed pattern-triggered immunity (PTI)-associated callose deposition. In addition, silencing of PSTha5a23 did not change Pst virulence phenotypes; however, overexpression of PSTha5a23 significantly enhanced Pst virulence in wheat. These results indicate that the Pst candidate effector PSTha5a23 plays an important role in plant defense suppression and rust pathogenicity, and also highlight the utility of gene overexpression in plants as a tool for studying effectors from obligate biotrophic pathogens. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

  14. Chimeric SV40 virus-like particles induce specific cytotoxicity and protective immunity against influenza A virus without the need of adjuvants.

    PubMed

    Kawano, Masaaki; Morikawa, Katsuma; Suda, Tatsuya; Ohno, Naohito; Matsushita, Sho; Akatsuka, Toshitaka; Handa, Hiroshi; Matsui, Masanori

    2014-01-05

    Virus-like particles (VLPs) are a promising vaccine platform due to the safety and efficiency. However, it is still unclear whether polyomavirus-based VLPs are useful for this purpose. Here, we attempted to evaluate the potential of polyomavirus VLPs for the antiviral vaccine using simian virus 40 (SV40). We constructed chimeric SV40-VLPs carrying an HLA-A*02:01-restricted, cytotoxic T lymphocyte (CTL) epitope derived from influenza A virus. HLA-A*02:01-transgenic mice were then immunized with the chimeric SV40-VLPs. The chimeric SV40-VLPs effectively induced influenza-specific CTLs and heterosubtypic protection against influenza A viruses without the need of adjuvants. Because DNase I treatment of the chimeric SV40-VLPs did not disrupt CTL induction, the intrinsic adjuvant property may not result from DNA contaminants in the VLP preparation. In addition, immunization with the chimeric SV40-VLPs generated long-lasting memory CTLs. We here propose that the chimeric SV40-VLPs harboring an epitope may be a promising CTL-based vaccine platform with self-adjuvant properties. © 2013 Elsevier Inc. All rights reserved.

  15. 48 CFR 23.801 - Authorities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Ozone-Depleting Substances 23.801 Authorities. (a) Title VI of the Clean Air Act... Environmental, Energy, and Transportation Management. (d) Executive Order 13514 of October 5, 2009, Federal...

  16. 48 CFR 23.801 - Authorities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE WORKPLACE Ozone-Depleting Substances 23.801 Authorities. (a) Title VI of the Clean Air Act... Environmental, Energy, and Transportation Management. (d) Executive Order 13514 of October 5, 2009, Federal...

  17. On the Samarium Substitution Effects in Y3-x Sm x Al5O12 (x = 0.1-3.0)

    NASA Astrophysics Data System (ADS)

    Skaudzius, Ramunas; Sakirzanovas, Simas; Kareiva, Aivaras

    2018-04-01

    Yttrium aluminium garnet substituted by samarium Y3-x Sm x Al5O12, (YSmAG, x = 0.1, 0.15, 0.25, 0.5, 0.75, 1.0, 1.5, 2.0, 2.5 and 3.0) was prepared by an aqueous sol-gel processing using etane-1,2-diol as complexing agent. The end products obtained at 1000°C in air were characterized by x-ray diffraction analysis, infrared spectroscopy (FT-IR) and scanning electron microscopy. It was demonstrated, however, that the total substitution of yttrium by samarium does not proceed in the YSmAG. The single cubic garnet phase was formed only at a low concentration of samarium (x = 0.1, 0.15, 0.25, 0.5, 0.75, 1.0). With further substitutional levels, if the amount of samarium was x = 1.5, 2.0, 2.5 and 3, respectively, the formation a of minor amount of side perovskite samarium aluminate SmAlO3 (SmAP) phase was observed. Surprisingly, when yttrium was totally replaced by the samarium (x = 3.0) the main synthesis product was SmAP. The possible formation of Sm3Al5O12 (SmAG) garnet was also investigated for the first time by variation of the temperature in the range of 780-835°C.

  18. SvO(2)-guided resuscitation for experimental septic shock: effects of fluid infusion and dobutamine on hemodynamics, inflammatory response, and cardiovascular oxidative stress.

    PubMed

    Rosário, André Loureiro; Park, Marcelo; Brunialti, Milena Karina; Mendes, Marialice; Rapozo, Marjorie; Fernandes, Denise; Salomão, Reinaldo; Laurindo, Francisco Rafael; Schettino, Guilherme Paula; Azevedo, Luciano Cesar P

    2011-12-01

    The pathogenetic mechanisms associated to the beneficial effects of mixed venous oxygen saturation (SvO(2))-guided resuscitation during sepsis are unclear. Our purpose was to evaluate the effects of an algorithm of SvO(2)-driven resuscitation including fluids, norepinephrine and dobutamine on hemodynamics, inflammatory response, and cardiovascular oxidative stress during a clinically resembling experimental model of septic shock. Eighteen anesthetized and catheterized pigs (35-45 kg) were submitted to peritonitis by fecal inoculation (0.75 g/kg). After hypotension, antibiotics were administered, and the animals were randomized to two groups: control (n = 9), with hemodynamic support aiming central venous pressure 8 to 12 mmHg, urinary output 0.5 mL/kg per hour, and mean arterial pressure greater than 65 mmHg; and SvO(2) (n = 9), with the goals above, plus SvO(2) greater than 65%. The interventions lasted 12 h, and lactated Ringer's and norepinephrine (both groups) and dobutamine (SvO(2) group) were administered. Inflammatory response was evaluated by plasma concentration of cytokines, neutrophil CD14 expression, oxidant generation, and apoptosis. Oxidative stress was evaluated by plasma and myocardial nitrate concentrations, myocardial and vascular NADP(H) oxidase activity, myocardial glutathione content, and nitrotyrosine expression. Mixed venous oxygen saturation-driven resuscitation was associated with improved systolic index, oxygen delivery, and diuresis. Sepsis induced in both groups a significant increase on IL-6 concentrations and plasma nitrate concentrations and a persistent decrease in neutrophil CD14 expression. Apoptosis rate and neutrophil oxidant generation were not different between groups. Treatment strategies did not significantly modify oxidative stress parameters. Thus, an approach aiming SvO(2) during sepsis improves hemodynamics, without any significant effect on inflammatory response and oxidative stress. The beneficial effects associated

  19. A Novel Highly Sensitive NO2 Sensor Based on Perovskite Na0.5+xBi0.5TiO3-δ Electrolyte.

    PubMed

    Xiao, Yihong; Zhang, Chufan; Zhang, Xu; Cai, Guohui; Zheng, Yong; Zheng, Ying; Zhong, Fulan; Jiang, Lilong

    2017-07-10

    NO x is one of dangerous air pollutants, and the demands for reliable sensors to detect NO x are extremely urgent recently. Conventional fluorite-phase YSZ used for NO x sensor requires higher operating temperature to obtain desirable oxygen ion conductivity. In this work, perovskite-phase Na 0.5 Bi 0.5 TiO 3 (NBT) oxygen conductor was chosen as the solid electrolyte to fabricate a novel highly sensitive NO 2 sensor with CuO as the sensing electrode and Pt as reference electrode. Na dopped Na 0.5 Bi 0.5 TiO 3 greatly improved the sensing performance of this sensor. The optimal sensor based on Na 0.51 Bi 0.50 TiO 3-δ exhibited good response-recovery characteristics to NO 2 and the response current values were almost linear to NO 2 concentrations in the range of 50-500 ppm at 400-600 °C. The response current value towards NO 2 reached maximum 11.23 μA at 575 °C and the value on NO 2 is much higher than other gases (CH 4 , C 2 H 4 , C 3 H 6 , C 3 H 8 , CO), indicating good selectivity for detecting NO 2 . The response signals of the sensor were slightly affected by coexistent O 2 varying from 2 to 21 vol% at 575 °C. The response current value decreased only 4.9% over 2 months, exhibiting the potential application in motor vehicles.

  20. 42 CFR 23.5 - What are the criteria for deciding which applications for assignment will be approved?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... development agency to which an application was submitted for review under § 23.4(c). (6) Comments received... applications for assignment will be approved? 23.5 Section 23.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT... Service Corps Personnel § 23.5 What are the criteria for deciding which applications for assignment will...

  1. Program Aids Creation Of X-Y Plots

    NASA Technical Reports Server (NTRS)

    Jeletic, James F.

    1993-01-01

    VEGAS computer program enables application programmers to create X-Y plots in various modes through high-level subroutine calls. Modes consist of passive, autoupdate, and interactive modes. In passive mode, VEGAS takes input data, produces plot, and returns control to application program. In autoupdate mode, forms plots and automatically updates them as more information received. In interactive mode, displays plot and provides popup menus for user to alter appearance of plot or to modify data. Written in FORTRAN 77.

  2. Electron energy loss spectroscopy analysis of lithium deintercalated Li5/3-xTi7/3CrO7

    NASA Astrophysics Data System (ADS)

    Díaz-Carrasco, P.; Moreau, P.; Guyomard, D.; Kuhn, A.; García-Alvarado, F.

    2006-05-01

    Electron energy loss spectroscopy has been used to investigate the average oxidation state of Cr in both electrochemically and chemically delithiathed Li5/3-xTi7/3CrO7. The O K edge, Ti L2,3 and Cr L2,3 edge were monitored. Upon removal of Li ions, the oxygen K edge shows a continuous change while the Cr L edges remain almost unchanged. The Cr 2p multiplet was compared with reference samples (Cr2O3, CrO2 and K2Cr2O7) in order to assess on the sensitivity of the Cr L-edge to the oxidation state of chromium ion. The similarity between the Cr L spectra of Cr2O3 and CrO2 makes the valence analysis difficult in Li5/3-xTi7/3CrO7 but the presence of CrVI is excluded in oxidized samples. However, evolution of the low energy loss spectra observed in the 10 15 eV region confirms the modification of the electronic structure and partial oxidation of CrIII to CrIV.

  3. Synthesis and antihyperlipaemic activity of some 2-aminomethyl-3-aryl-5,6,7,8-tetrahydrobenzo(b)/5,6-dimethylthieno++ +(2,3- d) -pyrimidin-4-ones.

    PubMed

    Gadad, A K; Kapsi, S G; Anegundi, R I; Pattan, S R; Mahajanshetti, C S; Shishoo, C J

    1996-10-01

    A series of 2-aminomethyl-3-aryl-5,6,7,8-tetrahydrobenzo(b)/5,6-dimethylthieno (2,3-d) pyrimidin-4-ones (IX) were prepared by the displacement reaction between various amines and 2-chloromethyl-3-aryl-5,6, 7,8-tetrahydrobenzo(b)/5, 6-dimethylthieno(2, 3-d) pyrimidin-4-ones (VIII), which are obtained by the cyclization of corresponding chloroacetylamino derivatives (VII) under acidic condition. Compounds VII were obtained by the interaction of VI and chloroacetylchloride in glacial acetic acid. Compounds VIII were converted to corresponding 2-acetoxymethyl derivatives (X) with potassium acetate in glacial acetic acid. Selected compounds were screened for antihyperlipaemic activity in albino rats, whereby most of these compounds were found to be active. The serum cholesterol and triglyceride lowering activities exhibited by compounds 1 and 3 were found to be comparable to that of gemfibrozil. Compounds 1 and 3 were also found to be safe as indicated by their acute toxicity study.

  4. Magnetic properties of x(Fe2O3).(100-x)[P2O5.Li2O] and x(Fe2O3).(100-x)[P2O5.CaO] glass systems

    NASA Astrophysics Data System (ADS)

    Andronache, Constantin; Racolta, Dania; Ardelean, Gheorghe

    2017-12-01

    Magnetic properties of x(Fe2O3).(100-x)[P2O5 .Li2O] and x(Fe2O3).(100-x)[P2O5 .CaO] with 0 < x ≤ 50 mol % were investigated using magnetic susceptibility measurements. The both glass systems were prepared in the same condition. The valence states and the distribution of iron ions in the glass matrix depend on the Fe2O3 content. For the P2O5.CaO glass matrix with x≤35mol%, the data revealed iron ions as isolated or participating in dipole-dipole interaction. For x > 35 mol% an antiferromagnetic coupling is observed. For the P2O5.Li2O glass matrix, the iron ions behave magnetically similarly as in other oxide glasses, but concentration of Fe2O3 over which magnetic superexchange interactions occur is lower. The absolute magnitude of θp values increases when content of Fe2O3 are increased. If the content of the magnetic ions is increased in the glass, the exchange integral increased and as a result the magnitude of the θP increases.

  5. Cationic distribution assisted tuning of magnetic properties of Li{sub 0.5-x/2}Zn{sub x}Fe{sub 2.5-x/2}O{sub 4}

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Panchal, S.; Raghuvanshi, S.; Gehlot, K.

    2016-05-15

    Zn addition induced modification of cationic distribution for tuning magnetic properties of Li{sub 0.5-x/2}Zn{sub x}Fe{sub 2.5-x/2}O{sub 4} (x = 0, 0.1, 0.25, 0.3, 0.5, 0.7, 0.9, 1.0) powders is studied by x-ray diffraction (XRD) and magnetic measurements. XRD shows the formation of cubic spinel structure, with Scherrer’s grain diameter ranging between 26.7 to 37.8 nm. With Zn addition, oxygen anions are displaced in such a way that the A-B interaction weakens, whereas A-A and B-B interaction strengthens; furthermore it also pushes Fe{sup 3+} and Li{sup 1+} ions to B site, leading to changes in magnetic properties. Highest saturation magnetization (M{submore » s}) of 64.6 A m{sup 2} / kg was obtained for sample with x = 0.25 and lowest coercivity (H{sub c}) of 4949.9 A/m was obtained for the sample with x = 0.7. Calculated values of experimental magnetic moment ‘n{sub B}’ and Neel magnetic moment ‘n{sub N}’, display similar trend as that of M{sub s}. Present results very clearly show a strong correlation between Zn-addition induced changes in cation distribution and magnetic properties, which can be utilized effectively for tuning magnetic properties.« less

  6. Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication

    PubMed Central

    Courtney, David G.

    2018-01-01

    Polyomaviruses are a family of small DNA tumor viruses that includes several pathogenic human members, including Merkel cell polyomavirus, BK virus and JC virus. As is characteristic of DNA tumor viruses, gene expression in polyomaviruses is temporally regulated into an early phase, consisting of the viral regulatory proteins, and a late phase, consisting of the viral structural proteins. Previously, the late transcripts expressed by the prototypic polyomavirus simian virus 40 (SV40) were reported to contain several adenosines bearing methyl groups at the N6 position (m6A), although the precise location of these m6A residues, and their phenotypic effects, have not been investigated. Here, we first demonstrate that overexpression of the key m6A reader protein YTHDF2 induces more rapid viral replication, and larger viral plaques, in SV40 infected BSC40 cells, while mutational inactivation of the endogenous YTHDF2 gene, or the m6A methyltransferase METTL3, has the opposite effect, thus suggesting a positive role for m6A in the regulation of SV40 gene expression. To directly test this hypothesis, we mapped sites of m6A addition on SV40 transcripts and identified two m6A sites on the viral early transcripts and eleven m6A sites on the late mRNAs. Using synonymous mutations, we inactivated the majority of the m6A sites on the SV40 late mRNAs and observed that the resultant viral mutant replicated more slowly than wild type SV40. Alternative splicing of SV40 late mRNAs was unaffected by the reduction in m6A residues and our data instead suggest that m6A enhances the translation of viral late transcripts. Together, these data argue that the addition of m6A residues to the late transcripts encoded by SV40 plays an important role in enhancing viral gene expression and, hence, replication. PMID:29447282

  7. A New SV2A Ligand for Epilepsy.

    PubMed

    Rogawski, Michael A

    2016-10-20

    Since the 1970s, racetams have been in use as cognitive enhancers. Levetiracetam was discovered to have antiseizure activity in animal models and was then found to bind to SV2A in synaptic and endocrine vesicles. Brivaracetam, an analog of levetiracetam, was identified in a medicinal chemistry campaign with the objective of discovering analogs with higher affinity at racetam-binding sites and greater antiseizure potency. Copyright © 2016. Published by Elsevier Inc.

  8. N-linked glycosylation of SV2 is required for binding and uptake of botulinum neurotoxin A.

    PubMed

    Yao, Guorui; Zhang, Sicai; Mahrhold, Stefan; Lam, Kwok-Ho; Stern, Daniel; Bagramyan, Karine; Perry, Kay; Kalkum, Markus; Rummel, Andreas; Dong, Min; Jin, Rongsheng

    2016-07-01

    Botulinum neurotoxin serotype A1 (BoNT/A1), a licensed drug widely used for medical and cosmetic applications, exerts its action by invading motoneurons. Here we report a 2.0-Å-resolution crystal structure of the BoNT/A1 receptor-binding domain in complex with its neuronal receptor, glycosylated human SV2C. We found that the neuronal tropism of BoNT/A1 requires recognition of both the peptide moiety and an N-linked glycan on SV2. This N-glycan-which is conserved in all SV2 isoforms across vertebrates-is essential for BoNT/A1 binding to neurons and for its potent neurotoxicity. The glycan-binding interface on SV2 is targeted by a human BoNT/A1-neutralizing antibody currently licensed as an antibotulism drug. Our studies reveal a new paradigm of host-pathogen interactions, in which pathogens exploit conserved host post-translational modifications, thereby achieving highly specific receptor binding while also tolerating genetic changes across multiple isoforms of receptors.

  9. N-linked glycosylation of SV2 is required for binding and uptake of botulinum neurotoxin A

    PubMed Central

    Yao, Guorui; Zhang, Sicai; Mahrhold, Stefan; Lam, Kwok-ho; Stern, Daniel; Bagramyan, Karine; Perry, Kay; Kalkum, Markus; Rummel, Andreas; Dong, Min; Jin, Rongsheng

    2016-01-01

    Botulinum neurotoxin serotype A1 (BoNT/A1) is one of the most dangerous potential bioterrorism agents, and exerts its action by invading motoneurons. It is also a licensed drug widely used for medical and cosmetic applications. Here we report a 2.0 Å resolution crystal structure of BoNT/A1 receptor-binding domain in complex with its neuronal receptor, the glycosylated human SV2C. We find that the neuronal tropism of BoNT/A1 requires recognition of both the peptide moiety and an N-linked glycan on SV2. This N-glycan—conserved in all SV2 isoforms across vertebrates—is essential for BoNT/A1 binding to neurons and its potent neurotoxicity. The glycan-binding interface on SV2 is targeted by a human BoNT/A1-neutralizing antibody currently licensed as an anti-botulism drug. Our studies reveal a new paradigm of host-pathogen interactions, in which pathogens exploit conserved host post-translational modifications to achieve highly specific receptor binding while also tolerating genetic changes across multiple isoforms of receptors. PMID:27294781

  10. Effect of non-magnetic ions substitution on the structure and magnetic properties of Y3-xSrxFe5-xZrxO12 nanoparticles

    NASA Astrophysics Data System (ADS)

    Mahmoudi, Soulmaz; Gholizadeh, Ahmad

    2018-06-01

    In this work, Y3-xSrxZrxFe5O12 (0.0 ≤ x ≤ 0.7) were synthesized by citrate precursor method at 1050 °C. The structural and magnetic properties of Y3-xSrxFe5-xZrxO12 were studied by using the X-ray diffraction technique, scanning electron microscopy, transmission electron microscopy, the Fourier transform infrared spectroscopy and vibrating sample magnetometer. XRD analysis using X'Pert package show a pure garnet phase with cubic structure (space group Ia-3d) and the impurity phase SrZrO3 is observed when the range of x value is exceeded from 0.6. Rietveld refinement using Fullprof program shows the lattice volume expansion with increasing the degree of Sr/Zr substitution. The crystallite sizes remain constant in the range of x = 0.0 - 0.5 and then increase. The different morphology observed in SEM micrographs of the samples can be related to different values of the microstrain in the samples. The hysteresis loops of the samples reveal a superparamagnetic behaviour. Also, the drop in coercivity with increasing of the substitution is mainly originated from a reduction in the magneto-elastic anisotropy energy. The values of the saturation magnetization (MS) indicate a non-monotonically variant with increasing the Sr/Zr substitution and reach a maximum 26.14 emu/g for the sample x = 0.1 and a minimum 17.64 emu/g for x = 0.0 and x = 0.2. The variation of MS, in these samples results from a superposition of three factors; reduction of Fe3+ in a-site, change in angle FeT-O-FeO, and magnetic core size.

  11. Charge-coupled Substituted Garnets (Y3-xCa0.5xM0.5x)Fe5O12 (M = Ce, Th): Structure and Stability as Crystalline Nuclear Waste Forms

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Guo, Xiaofeng; Kukkadapu, Ravi K.; Lanzirotti, Anthony

    2015-04-20

    The garnet structure has been proposed as a potential crystalline nuclear waste form for accommodation of actinide elements, especially uranium (U). In this study, yttrium iron garnet (YIG) as a model garnet host was studied for the incorporation of U analogs, cerium (Ce), and thorium (Th), incorporated by a charge-coupled substitution with calci-um (Ca) for yttrium (Y) in YIG, namely 2Y3+ = Ca2+ + M4+, where M4+ = Ce4+ or Th4+. Single phase garnets Y3-xCa0.5xM0.5xFe5O12, synthesized by the citrate-nitrate combustion method, were obtained up to x = 0.7. Ce was confirmed to be tetravalent by X-ray absorption spectroscopy and X-raymore » photoelectron spectroscopy. X-ray diffraction and 57Fe-Mössbauer spectroscopy indicated that the samples are single phase, M4+ and Ca2+ cations are restricted to the c-site, the nature of M4+ has only a minor effect on the structure, and the local environments of both the tetrahedral and octahedral Fe3+ are systematically affected by the extent of substitution, especially on the tetrahedral sublattice. The charge coupled substitution has advantages in incorporating Ce/Th and in stabilizing the substituted phases, compared to a single substitution strategy. Enthalpies of formation of garnets were obtained by high temperature oxide melt solution calorimetry, and the enthalpies of substitution of Ce and Th were determined. The thermodynamic analysis demonstrates that the substituted garnets are entropically rather than energetically stabilized. This suggests that such garnets may form and persist in repositories at high temperature but might decompose near room temperature. These structural and thermodynamic findings shed light on possible incorporation of U in this garnet system.« less

  12. 45 CFR 1306.23 - Training.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 4 2011-10-01 2011-10-01 false Training. 1306.23 Section 1306.23 Public Welfare... STAFFING REQUIREMENTS AND PROGRAM OPTIONS Head Start Program Staffing Requirements § 1306.23 Training. (a) Head Start grantees must provide pre-service training and in-service training opportunities to program...

  13. Low dose dynamic myocardial CT perfusion using advanced iterative reconstruction

    NASA Astrophysics Data System (ADS)

    Eck, Brendan L.; Fahmi, Rachid; Fuqua, Christopher; Vembar, Mani; Dhanantwari, Amar; Bezerra, Hiram G.; Wilson, David L.

    2015-03-01

    Dynamic myocardial CT perfusion (CTP) can provide quantitative functional information for the assessment of coronary artery disease. However, x-ray dose in dynamic CTP is high, typically from 10mSv to >20mSv. We compared the dose reduction potential of advanced iterative reconstruction, Iterative Model Reconstruction (IMR, Philips Healthcare, Cleveland, Ohio) to hybrid iterative reconstruction (iDose4) and filtered back projection (FBP). Dynamic CTP scans were obtained using a porcine model with balloon-induced ischemia in the left anterior descending coronary artery to prescribed fractional flow reserve values. High dose dynamic CTP scans were acquired at 100kVp/100mAs with effective dose of 23mSv. Low dose scans at 75mAs, 50mAs, and 25mAs were simulated by adding x-ray quantum noise and detector electronic noise to the projection space data. Images were reconstructed with FBP, iDose4, and IMR at each dose level. Image quality in static CTP images was assessed by SNR and CNR. Blood flow was obtained using a dynamic CTP analysis pipeline and blood flow image quality was assessed using flow-SNR and flow-CNR. IMR showed highest static image quality according to SNR and CNR. Blood flow in FBP was increasingly over-estimated at reduced dose. Flow was more consistent for iDose4 from 100mAs to 50mAs, but was over-estimated at 25mAs. IMR was most consistent from 100mAs to 25mAs. Static images and flow maps for 100mAs FBP, 50mAs iDose4, and 25mAs IMR showed comparable, clear ischemia, CNR, and flow-CNR values. These results suggest that IMR can enable dynamic CTP at significantly reduced dose, at 5.8mSv or 25% of the comparable 23mSv FBP protocol.

  14. Significant Radiation Dose Reduction in the Hybrid Operating Room Using a Novel X-ray Imaging Technology.

    PubMed

    van den Haak, R F F; Hamans, B C; Zuurmond, K; Verhoeven, B A N; Koning, O H J

    2015-10-01

    To prospectively quantify radiation dose change in aortoiliac endovascular procedures in the hybrid operating room (OR) for patients and medical staff with a novel X-ray imaging technology (ClarityIQ technology), and to assess whether procedure or fluoroscopy time or dose of iodinated contrast was affected. A prospective study including 138 patients was performed to compare radiation dose before and after installation of a novel X-ray imaging technology. Endovascular aneurysm repair (EVAR) was performed in 37 patients and an endovascular procedure for aortoiliac occlusive disease (AIOD) in 101. Patient radiation dose in air kerma (AK) and dose area product (DAP), patient demographics, and procedural data were recorded. Staff radiation dose was measured with real time personal dosimetry measurements. In both the EVAR and AIOD groups the reference system, ALX (AlluraXper FD20; Philips Healthcare, Best, the Netherlands), was compared with the upgraded X-ray system, CIQ (AlluraClarity FD20; Philips Healthcare). Procedure time, fluoroscopy time, and iodinated contrast dose were recorded. Patient radiation dose reduction in the EVAR group, in median AK, was 56% (ALX = 1,262.5 mGy; CIQ = 556.0 mGy [p < .01]); and in median DAP it was 57% (ALX = 224.4 Gycm(2) and CIQ = 95.8 Gycm(2) [p < .01]). Patient radiation dose reduction in the AIOD group, in median AK, was 76% (ALX = 1,011.0 mGy; CIQ = 248.0 mGy [p < .01]); and in median DAP it was 73% (ALX = 138.1 Gycm(2); CIQ = 38.0 Gycm(2) [p < .01]). Staff dose reduction in the EVAR group was 16% (ALX = 70.1 μSv; CIQ = 59.2 μSv [p = .43]) and in the AIOD group it was 69% (ALX = 96.2 μSv; CIQ = 30.1 μSv [p < .01]). There was no statistically significant difference between patient demographics, procedure time, fluoroscopy time, and iodinated contrast medium use in the two treatment groups before and after installation. A novel X-ray imaging technology in the hybrid OR suite resulted in a significant reduction of patient and

  15. 15 CFR 23.5 - Report to the Office of Juvenile Justice and Delinquency Prevention.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Office of Juvenile Justice and Delinquency Prevention. The Department of Commerce will compile and submit... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Report to the Office of Juvenile Justice and Delinquency Prevention. 23.5 Section 23.5 Commerce and Foreign Trade Office of the Secretary...

  16. 15 CFR 23.5 - Report to the Office of Juvenile Justice and Delinquency Prevention.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Report to the Office of Juvenile Justice and Delinquency Prevention. 23.5 Section 23.5 Commerce and Foreign Trade Office of the Secretary... Office of Juvenile Justice and Delinquency Prevention. The Department of Commerce will compile and submit...

  17. 15 CFR 23.5 - Report to the Office of Juvenile Justice and Delinquency Prevention.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Office of Juvenile Justice and Delinquency Prevention. The Department of Commerce will compile and submit... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Report to the Office of Juvenile Justice and Delinquency Prevention. 23.5 Section 23.5 Commerce and Foreign Trade Office of the Secretary...

  18. 15 CFR 23.5 - Report to the Office of Juvenile Justice and Delinquency Prevention.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Office of Juvenile Justice and Delinquency Prevention. The Department of Commerce will compile and submit... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Report to the Office of Juvenile Justice and Delinquency Prevention. 23.5 Section 23.5 Commerce and Foreign Trade Office of the Secretary...

  19. 15 CFR 23.5 - Report to the Office of Juvenile Justice and Delinquency Prevention.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Office of Juvenile Justice and Delinquency Prevention. The Department of Commerce will compile and submit... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Report to the Office of Juvenile Justice and Delinquency Prevention. 23.5 Section 23.5 Commerce and Foreign Trade Office of the Secretary...

  20. A 5-year evaluation of a smoking cessation incentive program for chemical employees.

    PubMed

    Olsen, G W; Lacy, S E; Sprafka, J M; Arceneaux, T G; Potts, T A; Kravat, B A; Gondek, M R; Bond, G G

    1991-11-01

    This 5-year study of the Dow Chemical Texas Operations 1984-1985 Smoking Cessation Incentive Program (SCIP) evaluated the smoking habits of 1,097 participants and 1,174 nonparticipants. We observed, via questionnaire and saliva cotinine data, that participants were 2.3 times more likely to be long-term (greater than or equal to 5 years) nonusers of tobacco than nonparticipants (10.2% vs 4.4%, P less than or equal to 0.01). However, smoking cessation rates for 3-4 years, 1-2 years, and less than 1 year were similar for participants who remained smokers at the conclusion of SCIP and nonparticipants. Age and the interaction between the management job category and having quit smoking for at least 30 days sometime prior to the worksite program were important predictors of smoking cessation among participants. Thirty-six percent of the participants who were considered exsmokers of 6 months duration at the conclusion of the program in 1985 remained long-term quitters 5 years later. Stress and enjoyment of smoking were the two most important reasons provided by participants for recidivism. The results of this 5-year evaluation demonstrate the heterogeneity of employee participation and success with a worksite smoking cessation program.

  1. 7 CFR 3403.5 - Program solicitation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 15 2012-01-01 2012-01-01 false Program solicitation. 3403.5 Section 3403.5 Agriculture Regulations of the Department of Agriculture (Continued) NATIONAL INSTITUTE OF FOOD AND AGRICULTURE SMALL BUSINESS INNOVATION RESEARCH GRANTS PROGRAM Preparation of Proposals § 3403.5 Program...

  2. 7 CFR 3403.5 - Program solicitation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false Program solicitation. 3403.5 Section 3403.5 Agriculture Regulations of the Department of Agriculture (Continued) NATIONAL INSTITUTE OF FOOD AND AGRICULTURE SMALL BUSINESS INNOVATION RESEARCH GRANTS PROGRAM Preparation of Proposals § 3403.5 Program...

  3. 7 CFR 3403.5 - Program solicitation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Program solicitation. 3403.5 Section 3403.5 Agriculture Regulations of the Department of Agriculture (Continued) NATIONAL INSTITUTE OF FOOD AND AGRICULTURE SMALL BUSINESS INNOVATION RESEARCH GRANTS PROGRAM Preparation of Proposals § 3403.5 Program...

  4. 7 CFR 3403.5 - Program solicitation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false Program solicitation. 3403.5 Section 3403.5 Agriculture Regulations of the Department of Agriculture (Continued) NATIONAL INSTITUTE OF FOOD AND AGRICULTURE SMALL BUSINESS INNOVATION RESEARCH GRANTS PROGRAM Preparation of Proposals § 3403.5 Program...

  5. Early superoxide dismutase alterations during SV40-transformation of human fibroblasts.

    PubMed

    Bravard, A; Hoffschir, F; Sabatier, L; Ricoul, M; Pinton, A; Cassingena, R; Estrade, S; Luccioni, C; Dutrillaux, B

    1992-11-11

    The expression of superoxide dismutases (SOD) 1 and 2 was studied in 4 clones of human fibroblasts after their infection by simian virus 40 (SV40), in parallel with the alterations of chromosomes 21 and chromosome 6q arms, carrying the genes that encode for SOD1 and SOD2 respectively. For all clones, a similar scheme with 2 main phases was observed for both chromosome and SOD variations. The first phase, defined as the pre-crisis phase, was characterized by chromosomal instability, but maintenance of normal numbers of chromosome 6q arms and chromosomes 21. The level of SOD2 mRNA was high, while SOD2 activity and immunoreactive protein were low. SOD1 protein and activity were decreased. In the second phase, defined as the post-crisis phase, the accumulation of clonal chromosomal rearrangements led to the loss of 6q arms, while the number of chromosomes 21 remained normal. SOD2 mRNA level was decreased and SOD2 immunoreactive protein and activity remained low. SOD1 protein and activity increased with passages, reaching values similar to those of control cells at late passages. As in established SV40-transformed human fibroblast cell lines, good correlation was found between SOD2 activity and the relative number of 6q arms. These results allow us to reconstruct the sequence of events leading to the decrease of SOD2, a possible tumor-suppressor gene, during the process of SV40-transformation of human fibroblasts.

  6. Measurement Invariance of the Short Version of the Problematic Mobile Phone Use Questionnaire (PMPUQ-SV) across Eight Languages.

    PubMed

    Lopez-Fernandez, Olatz; Kuss, Daria J; Pontes, Halley M; Griffiths, Mark D; Dawes, Christopher; Justice, Lucy V; Männikkö, Niko; Kääriäinen, Maria; Rumpf, Hans-Jürgen; Bischof, Anja; Gässler, Ann-Kathrin; Romo, Lucia; Kern, Laurence; Morvan, Yannick; Rousseau, Amélie; Graziani, Pierluigi; Demetrovics, Zsolt; Király, Orsolya; Schimmenti, Adriano; Passanisi, Alessia; Lelonek-Kuleta, Bernadeta; Chwaszcz, Joanna; Chóliz, Mariano; Zacarés, Juan José; Serra, Emilia; Dufour, Magali; Rochat, Lucien; Zullino, Daniele; Achab, Sophia; Landrø, Nils Inge; Suryani, Eva; Hormes, Julia M; Terashima, Javier Ponce; Billieux, Joël

    2018-06-08

    The prevalence of mobile phone use across the world has increased greatly over the past two decades. Problematic Mobile Phone Use (PMPU) has been studied in relation to public health and comprises various behaviours, including dangerous, prohibited, and dependent use. These types of problematic mobile phone behaviours are typically assessed with the short version of the Problematic Mobile Phone Use Questionnaire (PMPUQ⁻SV). However, to date, no study has ever examined the degree to which the PMPU scale assesses the same construct across different languages. The aims of the present study were to (i) determine an optimal factor structure for the PMPUQ⁻SV among university populations using eight versions of the scale (i.e., French, German, Hungarian, English, Finnish, Italian, Polish, and Spanish); and (ii) simultaneously examine the measurement invariance (MI) of the PMPUQ⁻SV across all languages. The whole study sample comprised 3038 participants. Descriptive statistics, correlations, and Cronbach's alpha coefficients were extracted from the demographic and PMPUQ-SV items. Individual and multigroup confirmatory factor analyses alongside MI analyses were conducted. Results showed a similar pattern of PMPU across the translated scales. A three-factor model of the PMPUQ-SV fitted the data well and presented with good psychometric properties. Six languages were validated independently, and five were compared via measurement invariance for future cross-cultural comparisons. The present paper contributes to the assessment of problematic mobile phone use because it is the first study to provide a cross-cultural psychometric analysis of the PMPUQ-SV.

  7. 77 FR 13318 - SV Hydro, LLC, Coffeeville, LLC, FFP Project 99, LLC; Notice Announcing Filing Priority for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-06

    ... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project Nos. 14298-000, 14299-000, 14301-000] SV Hydro, LLC, Coffeeville, LLC, FFP Project 99, LLC; Notice Announcing Filing Priority for... follows: 1. FFP Project 99, LLC: Project No. 14301-000. 2. SV Hydro, LLC: Project No. 14298-000. 3...

  8. Support to X-33/Resusable Launch Vehicle Technology Program

    NASA Technical Reports Server (NTRS)

    2000-01-01

    The X-33 Guidance, Navigation, and Control (GN&C) Peer Review Team (PRT) was formed to assess the integrated X-33 vehicle GN&C system in order to identify any areas of disproportionate risk for initial flight. The eventual scope of the PRT assessment encompasses the GN&C algorithms, software, avionics, control effectors, applicable models, and testing. The initial (phase 1) focus of the PRT was on the GN&C algorithms and the Flight Control Actuation Subsystem (FCAS). The PRT held meetings during its phase 1 assessment at X-33 assembly facilities in Palmdale, California on May 17-18, 2000 and at Honeywell facilities in Tempe, Arizona on June 7, 2000. The purpose of these meetings was for the PRT members to get background briefings on the X-33 vehicle and for the PRT team to be briefed on the design basis and current status of the X-33 GN&C algorithms as well as the FCAS. The following material is covered in this PRT phase 1 final report. Some significant GN&C-related accomplishments by the X-33 development team are noted. Some topics are identified that were found during phase 1 to require fuller consideration when the PRT reconvenes in the future. Some new recommendations by the PRT to the X-33 program will likely result from a thorough assessment of these subjects. An initial list of recommendations from the PRT to the X-33 program is provided. These recommendations stem from topics that received adequate review by the PRT in phase 1. Significant technical observations by the PRT members as a result of the phase 1 meetings are detailed. (These are covered in an appendix.) There were many X-33 development team members who contributed to the technical information used by the PRT during the phase 1 assessment, who supported presentations to the PRT, and who helped to address the many questions posed by the PRT members at and after the phase 1 meetings. In all instances the interaction between the PRT and the X-33 development team members was cordial and very

  9. 23 CFR 420.207 - What are the requirements for research, development, and technology transfer work programs?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Development and Technology Transfer Program Management § 420.207 What are the requirements for research, development, and technology transfer work programs? (a) The State DOT's RD&T work program must, as a minimum... 23 Highways 1 2013-04-01 2013-04-01 false What are the requirements for research, development, and...

  10. 23 CFR 420.207 - What are the requirements for research, development, and technology transfer work programs?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Development and Technology Transfer Program Management § 420.207 What are the requirements for research, development, and technology transfer work programs? (a) The State DOT's RD&T work program must, as a minimum... 23 Highways 1 2010-04-01 2010-04-01 false What are the requirements for research, development, and...

  11. 23 CFR 420.207 - What are the requirements for research, development, and technology transfer work programs?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Development and Technology Transfer Program Management § 420.207 What are the requirements for research, development, and technology transfer work programs? (a) The State DOT's RD&T work program must, as a minimum... 23 Highways 1 2014-04-01 2014-04-01 false What are the requirements for research, development, and...

  12. 23 CFR 420.207 - What are the requirements for research, development, and technology transfer work programs?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Development and Technology Transfer Program Management § 420.207 What are the requirements for research, development, and technology transfer work programs? (a) The State DOT's RD&T work program must, as a minimum... 23 Highways 1 2012-04-01 2012-04-01 false What are the requirements for research, development, and...

  13. 23 CFR 420.207 - What are the requirements for research, development, and technology transfer work programs?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Development and Technology Transfer Program Management § 420.207 What are the requirements for research, development, and technology transfer work programs? (a) The State DOT's RD&T work program must, as a minimum... 23 Highways 1 2011-04-01 2011-04-01 false What are the requirements for research, development, and...

  14. Attempts on producing lymphoid cell line from Penaeus monodon by induction with SV40-T and 12S EIA oncogenes.

    PubMed

    Puthumana, Jayesh; Prabhakaran, Priyaja; Philip, Rosamma; Singh, I S Bright

    2015-12-01

    In an attempt of in vitro transformation, transfection mediated expression of Simian virus-40 (T) antigen (SV40-T) and transduction mediated expression of Adenovirus type 12 early region 1A (12S E1A) oncogene were performed in Penaeus monodon lymphoid cells. pSV3-neo vector encoding SV40-T oncogene and a recombinant baculovirus BacP2-12S E1A-GFP encoding 12S E1A oncogene under the control of hybrid promoters were used. Electroporation and lipofection mediated transformation of SV40-T in lymphoid cells confirmed the transgene expression by phenotypic variation and the expression of GFP in co-transfection experiment. The cells transfected by lipofection (≥ 5%) survived for 14 days with lower toxicity (30%), whilst on electroporation, most of the cells succumbed to death (60%) and survived cells lived up to 7 days. Transduction efficiency in primary lymphoid cells was more than 80% within 14 days of post-transduction, however, an incubation period of 7 days post-transduction was observed without detectable expression of 12S E1A. High level of oncogenic 12S E1A expression were observed after 14 day post-transduction and the proliferating cells survived for more than 90 days with GFP expression, however, without in vitro transformation and immortalization. The study put forth the requirement of transduction mediated 'specific' oncogene expression along with telomerase activation and epigenetic induction for the immortalization and establishment of shrimp cell line. Copyright © 2015. Published by Elsevier Ltd.

  15. Correlations of crystallographic defects and anisotropy with magnetotransport properties in Fe x TaS 2 single crystals ( 0.23 ≤ x ≤ 0.35 )

    DOE PAGES

    Chen, Chih-Wei; Chikara, Shalinee; Zapf, Vivien S.; ...

    2016-08-02

    Very large magnetoresistance discovered in single crystals of the ferromagnetic Fe-intercalated transition metal dichalcogenide, Fe 0.28TaS 2 was attributed to the deviation of the Fe concentration from commensurate values (x = 1/4 or 1/3), which caused magnetic moment misalignments. Here we report a study of Fe xTaS 2 crystals with 0:23 ≤ x ≤ 0:35, demonstrating that crystallographic defects lead to spin disorder, which correlates with magneto-transport properties such as switching magnetic fi eld HS, magnetoresistance MR, and even zero-field resistivity ρ0 and temperature coefficient A in ρ(T) = ρ 0 + AT 2: The ordering temperature T C andmore » Weiss temperature θ W are maximized at the superstructure composition x = 1/4, while H s, MR, ρ 0, and A are minimum. Conversely, at a composition intermediate between the superstructure compositions x = 1/4 and 1/3, the corresponding magneto-transport properties reach local maxima.« less

  16. Correlations of crystallographic defects and anisotropy with magnetotransport properties in Fe x TaS 2 single crystals ( 0.23 ≤ x ≤ 0.35 )

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chen, Chih-Wei; Chikara, Shalinee; Zapf, Vivien S.

    Very large magnetoresistance discovered in single crystals of the ferromagnetic Fe-intercalated transition metal dichalcogenide, Fe 0.28TaS 2 was attributed to the deviation of the Fe concentration from commensurate values (x = 1/4 or 1/3), which caused magnetic moment misalignments. Here we report a study of Fe xTaS 2 crystals with 0:23 ≤ x ≤ 0:35, demonstrating that crystallographic defects lead to spin disorder, which correlates with magneto-transport properties such as switching magnetic fi eld HS, magnetoresistance MR, and even zero-field resistivity ρ0 and temperature coefficient A in ρ(T) = ρ 0 + AT 2: The ordering temperature T C andmore » Weiss temperature θ W are maximized at the superstructure composition x = 1/4, while H s, MR, ρ 0, and A are minimum. Conversely, at a composition intermediate between the superstructure compositions x = 1/4 and 1/3, the corresponding magneto-transport properties reach local maxima.« less

  17. Flight programs and X-ray optics development at MSFC

    NASA Astrophysics Data System (ADS)

    Gubarev, M.; Ramsey, B.; O'Dell, S.; Elsner, R.; Kilaru, K.; Atkins, C.; Swartz, D.; Gaskin, J.; Weisskopf, M.

    The X-ray astronomy group at the Marshall Space Flight Center (MSFC) is developing electroformed nickel/cobalt x-ray optics for suborbital and orbital experiments. Suborbital instruments include the Focusing X-ray Solar Imager (FOXSI) and Micro-X sounding rocket experiments and the HEROES balloon payload. Our current orbital program is the fabrication of mirror modules for the Astronomical Roentgen Telescope (ART) to be launched on board the Russian-German Spectrum Roentgen Gamma Mission (SRG). A second component of our work is the development of fabrication techniques and optical metrology to improve the angular resolution of thin-shell optics to the arcsecond-level.

  18. Treatment of X-Linked Hypophosphatemia with Calcitriol and Phosphate Increases Circulating Fibroblast Growth Factor 23 Concentrations

    PubMed Central

    Imel, Erik A.; DiMeglio, Linda A.; Hui, Siu L.; Carpenter, Thomas O.; Econs, Michael J.

    2010-01-01

    Context: X-Linked hypophosphatemia (XLH) is characterized by renal phosphate wasting, with inappropriately low or normal serum 1,25-dihydroxyvitamin D concentrations causing rickets and osteomalacia. Mutations in PHEX result in increased fibroblast growth factor 23 (FGF23) expression, elevating circulating FGF23 concentrations. Treating XLH with phosphate and calcitriol may further increase FGF23 concentrations, based on in vitro and in vivo models. Objective: The aim of the study was to investigate whether current standard XLH therapies increase circulating FGF23 concentrations. Design and Setting: We conducted a prospective observational study of XLH subjects during routine clinical management at two tertiary referral centers. Patients: The study included 10 XLH patients (seven children, three adults; age, 2–30 yr) initiating therapy and five XLH patients (age, 18–41 yr) electing not to undergo therapy. Intervention(s): Oral calcitriol and phosphate were administered. Main Outcome Measures: We measured circulating intact FGF23 concentrations. Results: Baseline circulating FGF23 concentrations were elevated in 14 of 15 subjects, increasing after treatment in most subjects. Follow-up was 14.4 ± 11.7 months (treatment cohort) and 25 ± 32 months (nontreatment cohort). FGF23 concentrations increased 132.7 ± 202.4% from pretreatment to peak during therapy but did not change significantly over time in the nontreatment cohort. FGF23 concentrations were related to phosphate doses (P = 0.04) and nonsignificantly to calcitriol doses (P = 0.06). Conclusions: Treating XLH with phosphate and calcitriol was associated with concurrent increases in circulating FGF23 concentrations, which may diminish therapeutic effect or contribute to complications of therapy. Because it is unknown whether the degree of FGF23 elevation correlates with disease severity in XLH, further study is needed to determine whether adjusting therapy to minimize effects on FGF23 concentration is

  19. Ferroelastic domain structure and phase transition in single-crystalline [PbZn 1/3Nb 2/3O 3] 1-x[PbTiO 3] x observed via in situ x-ray microbeam

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Li, Tao; Du, Zehui; Tamura, Nobumichi

    (1-x)Pb(Zn 1/3Nb 2/3)O 3-xPbTiO 3 ((1-x)PZN-xPT in short) is one of the most important piezoelectric materials. In this study, we extensively investigated (1-x)PZN-xPT (x = 0.07–0.11) ferroelectric single crystals using in-situ synchrotron μXRD, complemented by TEM and PFM, to correlate microstructures with phase transitions. The results reveal that (i) at 25°C, the equilibrium state of (1-x)PZN-xPT is a metastable orthorhombic phase for x = 0.07 and 0.08, while it shows coexistence of orthorhombic and tetragonal phases for x = 0.09 and x = 0.11, with all ferroelectric phases accompanied by ferroelastic domains; (ii) upon heating, the phase transformation in xmore » = 0.07 is Orthorhombic → Monoclinic → Tetragonal → Cubic. The coexistence of ferroelectric tetragonal and paraelectric cubic phases was in-situ observed in x = 0.08 above Curie temperature (T C), and (iii) phase transition can be explained by the evolution of the ferroelectric and ferroelastic domains. These results disclose that (1-x)PZN-xPT are in an unstable regime, which is possible factor for its anomalous dielectric response and high piezoelectric coefficient.« less

  20. Ferroelastic domain structure and phase transition in single-crystalline [PbZn 1/3Nb 2/3O 3] 1-x[PbTiO 3] x observed via in situ x-ray microbeam

    DOE PAGES

    Li, Tao; Du, Zehui; Tamura, Nobumichi; ...

    2017-11-10

    (1-x)Pb(Zn 1/3Nb 2/3)O 3-xPbTiO 3 ((1-x)PZN-xPT in short) is one of the most important piezoelectric materials. In this study, we extensively investigated (1-x)PZN-xPT (x = 0.07–0.11) ferroelectric single crystals using in-situ synchrotron μXRD, complemented by TEM and PFM, to correlate microstructures with phase transitions. The results reveal that (i) at 25°C, the equilibrium state of (1-x)PZN-xPT is a metastable orthorhombic phase for x = 0.07 and 0.08, while it shows coexistence of orthorhombic and tetragonal phases for x = 0.09 and x = 0.11, with all ferroelectric phases accompanied by ferroelastic domains; (ii) upon heating, the phase transformation in xmore » = 0.07 is Orthorhombic → Monoclinic → Tetragonal → Cubic. The coexistence of ferroelectric tetragonal and paraelectric cubic phases was in-situ observed in x = 0.08 above Curie temperature (T C), and (iii) phase transition can be explained by the evolution of the ferroelectric and ferroelastic domains. These results disclose that (1-x)PZN-xPT are in an unstable regime, which is possible factor for its anomalous dielectric response and high piezoelectric coefficient.« less

  1. 23 CFR 200.3 - Application of this part.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 23 Highways 1 2013-04-01 2013-04-01 false Application of this part. 200.3 Section 200.3 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION CIVIL RIGHTS TITLE VI PROGRAM AND RELATED... listed under § 200.5(p) of this part. Title VI requirements for 23 U.S.C. 402 will be covered under a...

  2. 23 CFR 200.3 - Application of this part.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 23 Highways 1 2014-04-01 2014-04-01 false Application of this part. 200.3 Section 200.3 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION CIVIL RIGHTS TITLE VI PROGRAM AND RELATED... listed under § 200.5(p) of this part. Title VI requirements for 23 U.S.C. 402 will be covered under a...

  3. 23 CFR 200.3 - Application of this part.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 23 Highways 1 2011-04-01 2011-04-01 false Application of this part. 200.3 Section 200.3 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION CIVIL RIGHTS TITLE VI PROGRAM AND RELATED... listed under § 200.5(p) of this part. Title VI requirements for 23 U.S.C. 402 will be covered under a...

  4. 23 CFR 200.3 - Application of this part.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Application of this part. 200.3 Section 200.3 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION CIVIL RIGHTS TITLE VI PROGRAM AND RELATED... listed under § 200.5(p) of this part. Title VI requirements for 23 U.S.C. 402 will be covered under a...

  5. Photoreduction of Carbon Dioxide to Methane Over Sb1.5Sn8.5-x Ti x O19.0 with High Conductivity.

    PubMed

    Do, Jeong Yeon; Kwak, Byeong Sub; Kang, Misook

    2018-09-01

    In order to enhance the photoreduction of CO2 to CH4, a new type of photocatalyst, Sb1.5Sn8.5-xTixO19.0, with high conductivity and low bandgap was developed by partially incorporating Ti into the framework of Sb1.5Sn8.5O19.0 (antimony-doped tin oxide, ATO) using a controlled hydrothermal method. XRD and TEM analyses indicated that the Sb1.5Sn8.5-xTixO19.0 particles exhibited a tetragonal crystal structure and were approximately 20 nm in size. Furthermore, the bandgap and conductivity of these materials increased with increasing Ti content. A study of the photoreduction of CO2 with H2O revealed a remarkable increase in the generation of CH4 over the Sb1.5Sn8.5-xTixO19.0 catalysts. In particular, CH4 generation was the highest when Sb1.5Sn8.5Ti1.0O19.0 was used as the photocatalyst, and was three-fold higher than that achieved by using anatase TiO2. Photoluminescence studies showed that the enhanced photocatalytic activity of the Sb1.5Sn8.5-xTixO19.0 materials could be attributed to the interfacial transfer of photogenerated charges, which led to an effective charge separation and inhibition of the recombination of photogenerated electron-hole (e-/h+) pairs.

  6. Glycosylated SV2 and Gangliosides as Dual Receptors for Botulinum Neurotoxin Serotype F

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fu, Zhuji; Chen, Chen; Barbieri, Joseph T.

    2010-02-22

    Botulinum neurotoxin causes rapid flaccid paralysis through the inhibition of acetylcholine release at the neuromuscular junction. The seven BoNT serotypes (A-G) have been proposed to bind motor neurons via ganglioside-protein dual receptors. To date, the structure-function properties of BoNT/F host receptor interactions have not been resolved. Here, we report the crystal structures of the receptor binding domains (HCR) of BoNT/A and BoNT/F and the characterization of the dual receptors for BoNT/F. The overall polypeptide fold of HCR/A is essentially identical to the receptor binding domain of the BoNT/A holotoxin, and the structure of HCR/F is very similar to that ofmore » HCR/A, except for two regions implicated in neuronal binding. Solid phase array analysis identified two HCR/F binding glycans: ganglioside GD1a and oligosaccharides containing an N-acetyllactosamine core. Using affinity chromatography, HCR/F bound native synaptic vesicle glycoproteins as part of a protein complex. Deglycosylation of glycoproteins using {alpha}(1-3,4)-fucosidase, endo-{beta}-galactosidase, and PNGase F disrupted the interaction with HCR/F, while the binding of HCR/B to its cognate receptor, synaptotagmin I, was unaffected. These data indicate that the HCR/F binds synaptic vesicle glycoproteins through the keratan sulfate moiety of SV2. The interaction of HCR/F with gangliosides was also investigated. HCR/F bound specifically to gangliosides that contain {alpha}2,3-linked sialic acid on the terminal galactose of a neutral saccharide core (binding order GT1b = GD1a GM3; no binding to GD1b and GM1a). Mutations within the putative ganglioside binding pocket of HCR/F decreased binding to gangliosides, synaptic vesicle protein complexes, and primary rat hippocampal neurons. Thus, BoNT/F neuronal discrimination involves the recognition of ganglioside and protein (glycosylated SV2) carbohydrate moieties, providing a structural basis for the high affinity and specificity of BoNT/F for

  7. affy2sv: an R package to pre-process Affymetrix CytoScan HD and 750K arrays for SNP, CNV, inversion and mosaicism calling.

    PubMed

    Hernandez-Ferrer, Carles; Quintela Garcia, Ines; Danielski, Katharina; Carracedo, Ángel; Pérez-Jurado, Luis A; González, Juan R

    2015-05-20

    The well-known Genome-Wide Association Studies (GWAS) had led to many scientific discoveries using SNP data. Even so, they were not able to explain the full heritability of complex diseases. Now, other structural variants like copy number variants or DNA inversions, either germ-line or in mosaicism events, are being studies. We present the R package affy2sv to pre-process Affymetrix CytoScan HD/750k array (also for Genome-Wide SNP 5.0/6.0 and Axiom) in structural variant studies. We illustrate the capabilities of affy2sv using two different complete pipelines on real data. The first one performing a GWAS and a mosaic alterations detection study, and the other detecting CNVs and performing an inversion calling. Both examples presented in the article show up how affy2sv can be used as part of more complex pipelines aimed to analyze Affymetrix SNP arrays data in genetic association studies, where different types of structural variants are considered.

  8. 5 CFR 1208.23 - Content of appeal; request for hearing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Content of appeal; request for hearing... PROCEDURES PRACTICES AND PROCEDURES FOR APPEALS UNDER THE UNIFORMED SERVICES EMPLOYMENT AND REEMPLOYMENT RIGHTS ACT AND THE VETERANS EMPLOYMENT OPPORTUNITIES ACT VEOA Appeals § 1208.23 Content of appeal...

  9. The 5'-untranslated region of p23 mRNA from the Ehrlich ascites tumor is involved in translation control of the growth related protein p23.

    PubMed

    Böhm, H; Gross, B; Gaestel, M; Bommer, U A; Ryffel, G; Bielka, H

    1991-01-01

    The growth-related protein p23 of the Ehrlich ascites tumor (EAT) is preferentially expressed in the exponentially growing tumor; its synthesis is translationally controlled. p23 mRNA is efficiently translated in the wheat germ cell-free lysate. In contrast, p23 mRNA present in poly(A)+RNA isolated from EAT is not translated in cell-free systems of EAT and reticulocytes. Moreover, translation of a p23 transcript is inhibited in the presence of total poly(A)+RNA. This inhibition is abolished by the removal of the 5'-UTR of the p23 transcript. Solution hybridization/RNase protection experiments point to the presence of a nucleotide sequence complementary to the 5'-UTR of p23 mRNA which might be involved in p23 mRNA inhibition.

  10. Ambient x-ray pollution assessment at inspection gates of airports- a case study of Mehrabad and Imam Khomeini Airports in Iran

    PubMed Central

    2014-01-01

    Background As a well-known, physical carcinogen, ambient X-ray pollution assessment would be of great importance in today’s modern world. Accordingly, the present study was done to measure the exposure level of ambient X-ray at inspection gates of two airports in Iran. According to which, the X-ray was measured at different points of the inspection gates including closed and opened Curtain, as well as seating place of operators beside the X-ray inspection systems. The recorded data were then analyzed by “sign” and t-tests. Results The total average exposure level of the measured x-ray was 2.68 ± 0.73 μsv.h-1. The measured x-ray exposure level was 2.07 ± 0.61 (μsv.h-1) released from RAPISCAN X-ray inspection system and 3.3 ± 1.34 (μsv.h-1) emitted from HEIMANN X-ray inspection system. Comparison of average x-ray doses of the systems in both airports showed that the minimum and maximum exposure levels were recorded at 1(m) far from the devices and at the entrance of the devices, respectively. Conclusions The exposure levels at all measurement points were lower than the occupational exposure limit. This reveals the fact that the exposed operators are not probably at risk of adverse health effects. PMID:24955243

  11. Ambient x-ray pollution assessment at inspection gates of airports- a case study of Mehrabad and Imam Khomeini Airports in Iran.

    PubMed

    Pourtaghi, Gholamhossein; Valipour, Firouz; Nourian, Sepideh; Mofidi, Amirabbas

    2014-01-01

    As a well-known, physical carcinogen, ambient X-ray pollution assessment would be of great importance in today's modern world. Accordingly, the present study was done to measure the exposure level of ambient X-ray at inspection gates of two airports in Iran. According to which, the X-ray was measured at different points of the inspection gates including closed and opened Curtain, as well as seating place of operators beside the X-ray inspection systems. The recorded data were then analyzed by "sign" and t-tests. The total average exposure level of the measured x-ray was 2.68 ± 0.73 μsv.h(-1). The measured x-ray exposure level was 2.07 ± 0.61 (μsv.h(-1)) released from RAPISCAN X-ray inspection system and 3.3 ± 1.34 (μsv.h(-1)) emitted from HEIMANN X-ray inspection system. Comparison of average x-ray doses of the systems in both airports showed that the minimum and maximum exposure levels were recorded at 1(m) far from the devices and at the entrance of the devices, respectively. The exposure levels at all measurement points were lower than the occupational exposure limit. This reveals the fact that the exposed operators are not probably at risk of adverse health effects.

  12. Supergiant X-Ray Binaries Observed by Suzaku

    NASA Technical Reports Server (NTRS)

    Bodaghee, A.; Tomsick, J. A.; Rodriquez, J.; Chaty, S.; Pottschmidt, K.; Walter, R.; Romano, P.

    2011-01-01

    Suzaku observations are presented for the high-mass X-ray binaries IGR 116207-5129 and IGR 117391-3021. For IGR 116207-5129, we provide the first X-ray broadband (0.5-60 keV) spectrum from which we confirm a large intrinsic column density (N(sub H) = 1.6 x 10(exp 23)/sq cm), and we constrain the cutoff energy for the first time (E(sub cut) = 19 keV). A prolonged (> 30 ks) attenuation of the X-ray flux was observed which we tentatively attribute to an eclipse of the probable neutron star by its massive companion, in a binary system with an orbital period between 4 and 9 days, and inclination angles> 50 degrees. For IGRJ17391-3021, we witnessed a transition from quiescence to a low-activity phase punctuated by weak flares whose peak luminosities in the 0.5-10keV band are only a factor of 5 times that of the pre-flare emission. These micro flares are accompanied by an increase in NH which suggests the accretion of obscuring clumps of wind. We now recognize that these low-activity epochs constitute the most common emission phase for this system, and perhaps in other supergiant fast X-ray transients (SFXTs) as well. We close with an overview of our upcoming program in which Suzaku will provide the first ever observation of an SFXT (IGRJ16479-4514) during a binary orbit enabling us to probe the accretion wind at every phase.

  13. TES/Aura L3 Atmospheric Temperatures Daily V5 (TL3ATD)

    Atmospheric Science Data Center

    2018-05-08

    ... Platform:  TES Aura L1B Nadir/Limb Spatial Coverage:  (-180, 180)(-90, 90) Spatial Resolution:  0.5 x 5 km nadir 2.3 x 23 km limb Temporal Coverage:  07/15/2004 - Present Temporal Resolution:  ...

  14. Coal-seismic, desktop computer programs in BASIC; Part 5, Perform X-square T-square analysis and plot normal moveout lines on seismogram overlay

    USGS Publications Warehouse

    Hasbrouck, W.P.

    1983-01-01

    Processing of data taken with the U.S. Geological Survey's coal-seismic system is done with a desktop, stand-alone computer. Programs for this computer are written in the extended BASIC language used by the Tektronix 4051 Graphic System. This report presents computer programs to perform X-square/T-square analyses and to plot normal moveout lines on a seismogram overlay.

  15. A Long Look at MCG-5-23-16 with NuSTAR. I. Relativistic Reflection and Coronal Properties

    NASA Technical Reports Server (NTRS)

    Zoghbi, Abderahmen; Matt, G.; Miller, J. M.; Lohfink, A. M.; Walton, D. J.; Ballantyne, D. R.; Garcia, J. A.; Stern, D.; Koss, M. J.; Farrah, D.; hide

    2017-01-01

    MCG-5-23-16 was targeted in early 2015 with a half mega-second observing campaign using NuSTAR. Here we present the spectral analysis of these data sets along with an earlier observation and study the relativistic reflection and the primary coronal source. The data show strong reflection features in the form of both narrow and broad iron lines plus a Compton reflection hump. A cutoff energy is significantly detected in all exposures. The shape of the reflection spectrum does not change in the two years spanned by the observations, suggesting a stable geometry. A strong positive correlation is found between the cutoff energy and both the hard X-ray flux and spectral index. The measurements imply that the coronal plasma is not at the runaway electron-positron pair limit, and instead contains mostly electrons. The observed variability in the coronal properties is driven by a variable optical depth. A constant heating-to-cooling ratio is measured, implying that there is a feedback mechanism in which a significant fraction of the photons cooling the corona are due to reprocessed hard X-rays.

  16. A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1.

    PubMed

    Fayed, Bahgat; Younger, Ellen; Taylor, Gabrielle; Smith, Margaret C M

    2014-05-30

    Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.

  17. 10 CFR 26.23 - Performance objectives.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Performance objectives. 26.23 Section 26.23 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Program Elements § 26.23 Performance objectives. Fitness-for-duty programs must— (a) Provide reasonable assurance that individuals are trustworthy and reliable as...

  18. 10 CFR 26.23 - Performance objectives.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Performance objectives. 26.23 Section 26.23 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Program Elements § 26.23 Performance objectives. Fitness-for-duty programs must— (a) Provide reasonable assurance that individuals are trustworthy and reliable as...

  19. 10 CFR 26.23 - Performance objectives.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Performance objectives. 26.23 Section 26.23 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Program Elements § 26.23 Performance objectives. Fitness-for-duty programs must— (a) Provide reasonable assurance that individuals are trustworthy and reliable as...

  20. 10 CFR 26.23 - Performance objectives.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Performance objectives. 26.23 Section 26.23 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Program Elements § 26.23 Performance objectives. Fitness-for-duty programs must— (a) Provide reasonable assurance that individuals are trustworthy and reliable as...

  1. 10 CFR 26.23 - Performance objectives.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Performance objectives. 26.23 Section 26.23 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Program Elements § 26.23 Performance objectives. Fitness-for-duty programs must— (a) Provide reasonable assurance that individuals are trustworthy and reliable as...

  2. SV2 frustrating exocytosis at the semi-diffusor synapse.

    PubMed

    Vautrin, Jean

    2009-04-01

    Presynaptic exocytosis is the mechanism commonly believed to release transmitters by diffusion through a pore opening during vesicular membrane fusion with the plasmalemma, but evidence suggesting that exocytosis and transmitter release are two separate steps of synaptic transmission is accumulating. Vesicular glycoconjugates such as Synaptic Vesicle Protein 2 (SV2) proteoglycans and gangliosides retain transmitters in a nondiffusible form and are transported to the synaptic cleft where they contribute forming a dense synaptomatrix. Transmitters are permanently present in synaptic clefts and readily releasable transmitter is easily accessible from the outer side of the presynaptic membrane suggesting that synaptomatrix glycoconjugates prevent immediate release after PKC-dependent exocytosis. The calcium sensor synaptotagmin is also present at the presynaptic plasma membrane and binds SV2 suggesting a direct coupling between the calcium transient and transmitter release from the synaptomatrix. A quantitative coupling of the cytosolic calcic transient to transmitter release from the synaptomatrix explains better complexity and plasticity of miniature postsynaptic signals hitherto difficult to account for in exocytic terms. This alternative representation of synaptic transmission in which the same components of the synaptomatrix support adhesion and signaling functions may cast new lights on synaptic diseases such as Alzheimer's disease. Copyright 2008 Wiley-Liss, Inc.

  3. Evaluation and comparision of dc resistivity of NiZr x Co x Fe2-2x O4, Ni0.5Sn0.5Co x Mn x Fe2-2x O4, Mg1-x Ca x Ni y Fe2-y O4 and Mg1-x Ni x Co y Fe2-y O4 nanocrytalline materials

    NASA Astrophysics Data System (ADS)

    Ali, Rajjab; Gilani, Zaheer Abbas; Shahzad Shifa, Muhammad; Asghar, H. M. Noor Ul Huda Khan; Azhar Khan, Muhammad; Naeem Anjum, Muhammad; Nauman Usmani, Muhammad; Farooq Warsi, Muhammad; Khawaja, Imtiaz U.

    2017-11-01

    Four series nanocrystalline ferrites with nominal composition, NiZr x Co x Fe2-2x O4 (x  =  0.0, 0.2, 0.4, 0.6, 0.8) Ni0.5Sn0.5Co x Mn x Fe2-2x O4 (x  =  0.0, 0.2, 0.4, 0.6, 0.8), Mg1-x Ca x Ni y Fe2-y O4 (x  =  0.0, 0.2, 0.4, 0.6, 0.8; y  =  0, 04, 0.8, 1.2, 1.6) and Mg1-x Ni x Co y Fe2-y O4 (x,y  =  0.0, 0.2, 0.4, 0.6, 0.8) have been fabricated using the microemulsion synthesis route. The synthesized materials are investigated for dc electrical resistivity measurements. The variation of dc electrical resistivity of these materials has been explainedon the basis of hopping mechanism of both holes and electrons.

  4. XAP, a program for deconvolution and analysis of complex X-ray spectra

    USGS Publications Warehouse

    Quick, James E.; Haleby, Abdul Malik

    1989-01-01

    The X-ray analysis program (XAP) is a spectral-deconvolution program written in BASIC and specifically designed to analyze complex spectra produced by energy-dispersive X-ray analytical systems (EDS). XAP compensates for spectrometer drift, utilizes digital filtering to remove background from spectra, and solves for element abundances by least-squares, multiple-regression analysis. Rather than base analyses on only a few channels, broad spectral regions of a sample are reconstructed from standard reference spectra. The effects of this approach are (1) elimination of tedious spectrometer adjustments, (2) removal of background independent of sample composition, and (3) automatic correction for peak overlaps. Although the program was written specifically to operate a KEVEX 7000 X-ray fluorescence analytical system, it could be adapted (with minor modifications) to analyze spectra produced by scanning electron microscopes, electron microprobes, and probes, and X-ray defractometer patterns obtained from whole-rock powders.

  5. Synthesis and characterization of p-type conductivity dopant 2-(3-(adamantan-1-yl)propyl)-3,5,6-trifluoro-7,7,8,8-tetracyanoquinodimethane

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rainbolt, James E.; Koech, Phillip K.; Polikarpov, Evgueni

    2013-01-22

    We report the synthesis and characterization of 2-(3-(adamantan-1-yl)propyl)-3,5,6-trifluoro-7,7,8,8-tetracyanoquinodimethane (F3TCNQ-Ad1), a substituted analog of 2,3,5,6-tetrafluoro-7,7,8,8-tetracyanoquinodimethane (F4TCNQ), designed for p-type conductivity doping. The dopant is designed as a model for substituted alternatives to F4TCNQ that maintain similar electronic properties with the goal of engineering dopants with superior fabrication characteristics over F4TCNQ. We describe the design strategy for F3TCNQ-Ad1 based on molecular modeling predictions that substitution of a single fluorine atom of F4TCNQ has little effect on the electronic properties of the molecule. Photophysical and electrochemical characterization reveal that the adamantyl substituent in F3TCNQ-Ad1 does not significantly alter the electronic properties of themore » substituted dopant relative to F4TCNQ. Unfortunately, F3TCNQ-Ad1 degrades under standard sublimation conditions, preventing sublimation deposition processing. Instead, hole-only devices were made via solution-processing of the p-doped films with the structure glass/ITO/2.3 x103Å PVK:(MTDATA:dopant)/2.0x102Å Au/1.0x103Å Al, where dopant is either F4TCNQ or F3TCNQ-Ad1. We demonstrate that F3TCNQ-Ad1 increased the conductivity of the films by at least 1,000 times compared to an undoped device.« less

  6. The CBL-Interacting Protein Kinase CIPK23 Regulates HAK5-Mediated High-Affinity K+ Uptake in Arabidopsis Roots.

    PubMed

    Ragel, Paula; Ródenas, Reyes; García-Martín, Elena; Andrés, Zaida; Villalta, Irene; Nieves-Cordones, Manuel; Rivero, Rosa M; Martínez, Vicente; Pardo, Jose M; Quintero, Francisco J; Rubio, Francisco

    2015-12-01

    Plant growth and development requires efficient acquisition of essential elements. Potassium (K(+)) is an important macronutrient present in the soil solution at a wide range of concentrations. Regulation of the K(+) uptake systems in the roots is essential to secure K(+) supply. It has been shown in Arabidopsis (Arabidopsis thaliana) that when the external K(+) concentration is very low (<10 µm), K(+) nutrition depends exclusively on the high-affinity K(+) transporter5 (HAK5). Low-K(+)-induced transcriptional activation of the gene encoding HAK5 has been previously reported. Here, we show the posttranscriptional regulation of HAK5 transport activity by phosphorylation. Expression in a heterologous system showed that the Ca(2+) sensors calcineurin B-like (CBL1), CBL8, CBL9, and CBL10, together with CBL-interacting protein kinase23 (CIPK23), activated HAK5 in vivo. This activation produced an increase in the affinity and the Vmax of K(+) transport. In vitro experiments show that the N terminus of HAK5 is phosphorylated by CIPK23. This supports the idea that phosphorylation of HAK5 induces a conformational change that increases its affinity for K(+). Experiments of K(+) (Rb(+)) uptake and growth measurements in low-K(+) medium with Arabidopsis single mutants hak5, akt1, and cipk23, double mutants hak5 akt1, hak5 cipk23, and akt1 cipk23, and the triple mutant hak5 akt1 cipk23 confirmed the regulatory role of CIPK23 in planta. © 2015 American Society of Plant Biologists. All Rights Reserved.

  7. 7 CFR 773.23 - Exception.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Exception. 773.23 Section 773.23 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM § 773.23 Exception. The Agency may grant an exception to the...

  8. 7 CFR 773.23 - Exception.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 7 2013-01-01 2013-01-01 false Exception. 773.23 Section 773.23 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM § 773.23 Exception. The Agency may grant an exception to the...

  9. 7 CFR 773.23 - Exception.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Exception. 773.23 Section 773.23 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM § 773.23 Exception. The Agency may grant an exception to the...

  10. 7 CFR 773.23 - Exception.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false Exception. 773.23 Section 773.23 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM § 773.23 Exception. The Agency may grant an exception to the...

  11. 7 CFR 773.23 - Exception.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Exception. 773.23 Section 773.23 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM § 773.23 Exception. The Agency may grant an exception to the...

  12. 7 CFR 23.10 - Administration.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Administration. 23.10 Section 23.10 Agriculture Office of the Secretary of Agriculture STATE AND REGIONAL ANNUAL PLANS OF WORK Regional Program § 23.10 Administration. (a) The Regional Programs will be administered through four Regional Rural Development Centers...

  13. 7 CFR 23.10 - Administration.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Administration. 23.10 Section 23.10 Agriculture Office of the Secretary of Agriculture STATE AND REGIONAL ANNUAL PLANS OF WORK Regional Program § 23.10 Administration. (a) The Regional Programs will be administered through four Regional Rural Development Centers...

  14. 7 CFR 23.10 - Administration.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Administration. 23.10 Section 23.10 Agriculture Office of the Secretary of Agriculture STATE AND REGIONAL ANNUAL PLANS OF WORK Regional Program § 23.10 Administration. (a) The Regional Programs will be administered through four Regional Rural Development Centers...

  15. Analysis of X-ray adsorption edges: L 2,3 edge of FeCl 4 -

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bagus, Paul S.; Nelin, Connie J.; Ilton, Eugene S.

    We describe a detailed analysis of the features of the X-ray adsorption spectra at the Fe L 2,3 edge of FeCl 4. The objective of this analysis is to explain the origin of the complex features in relation to properties of the wavefunctions, especially for the excited states. These properties include spin-orbit and ligand field splittings where a novel aspect of the dipole selection rules is applied to understand the influence of these splittings on the spectra. We also explicitly take account of the intermediate coupling of the open core and valence shell electrons. Our analysis also includes comparison ofmore » theory and experiment for the Fe L 2,3 edge and comparison of theoretical predictions for the Fe 3+ cation and FeCl 4-. The electronic structure is obtained from theoretical wavefunctions for the ground and excited states.« less

  16. MBD5 haploinsufficiency is associated with sleep disturbance and disrupts circadian pathways common to Smith-Magenis and fragile X syndromes.

    PubMed

    Mullegama, Sureni V; Pugliesi, Loren; Burns, Brooke; Shah, Zalak; Tahir, Raiha; Gu, Yanghong; Nelson, David L; Elsea, Sarah H

    2015-06-01

    Individuals with autism spectrum disorders (ASD) who have an identifiable single-gene neurodevelopmental disorder (NDD), such as fragile X syndrome (FXS, FMR1), Smith-Magenis syndrome (SMS, RAI1), or 2q23.1 deletion syndrome (del 2q23.1, MBD5) share phenotypic features, including a high prevalence of sleep disturbance. We describe the circadian deficits in del 2q23.1 through caregiver surveys in which we identify several frequent sleep anomalies, including night/early awakenings, coughing/snoring loudly, and difficulty falling asleep. We couple these findings with studies on the molecular analysis of the circadian deficits associated with haploinsufficiency of MBD5 in which circadian gene mRNA levels of NR1D2, PER1, PER2, and PER3 were altered in del 2q23.1 lymphoblastoid cell lines (LCLs), signifying that haploinsufficiency of MBD5 can result in dysregulation of circadian rhythm gene expression. These findings were further supported by expression microarrays of MBD5 siRNA knockdown cells that showed significantly altered expression of additional circadian rhythm signaling pathway genes. Based on the common sleep phenotypes observed in del 2q23.1, SMS, and FXS patients, we explored the possibility that MBD5, RAI1, and FMR1 function in overlapping circadian rhythm pathways. Bioinformatic analysis identified conserved putative E boxes in MBD5 and RAI1, and expression levels of NR1D2 and CRY2 were significantly reduced in patient LCLs. Circadian and mTOR signaling pathways, both associated with sleep disturbance, were altered in both MBD5 and RAI1 knockdown microarray data, overlapping with findings associated with FMR1. These data support phenotypic and molecular overlaps across these syndromes that may be exploited to provide therapeutic intervention for multiple disorders.

  17. Electric field dependent local structure of ( K x N a 1 - x ) 0.5 B i 0.5 Ti O 3

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Goetzee-Barral, A. J.; Usher, T. -M.; Stevenson, T. J.

    The in situ x-ray pair-distribution function (PDF) characterization technique has been used to study the behavior of (K xNa 1–x) 0.5Bi 0.5TiO 3, as a function of electric field. As opposed to conventional x-ray Bragg diffraction techniques, PDF is sensitive to local atomic displacements, detecting local structural changes at the angstrom to nanometer scale. Several field-dependent ordering mechanisms can be observed in x = 0.15, 0.18 and at the morphotropic phase boundary composition x = 0.20. X-ray total scattering shows suppression of diffuse scattering with increasing electric-field amplitude, indicative of an increase in structural ordering. Analysis of PDF peaks inmore » the 3–4-Å range shows ordering of Bi-Ti distances parallel to the applied electric field, illustrated by peak amplitude redistribution parallel and perpendicular to the electric-field vector. A transition from < 110 > to < 112 >-type off-center displacements of Bi relative to the neighboring Ti atoms is observable with increasing x. Analysis of PDF peak shift with electric field shows the effects of Bi-Ti redistribution and onset of piezoelectric lattice strain. Furthermore, the combination of these field-induced ordering mechanisms is consistent with local redistribution of Bi-Ti distances associated with domain reorientation and an overall increase in order of atomic displacements.« less

  18. Electric field dependent local structure of ( K x N a 1 - x ) 0.5 B i 0.5 Ti O 3

    DOE PAGES

    Goetzee-Barral, A. J.; Usher, T. -M.; Stevenson, T. J.; ...

    2017-07-31

    The in situ x-ray pair-distribution function (PDF) characterization technique has been used to study the behavior of (K xNa 1–x) 0.5Bi 0.5TiO 3, as a function of electric field. As opposed to conventional x-ray Bragg diffraction techniques, PDF is sensitive to local atomic displacements, detecting local structural changes at the angstrom to nanometer scale. Several field-dependent ordering mechanisms can be observed in x = 0.15, 0.18 and at the morphotropic phase boundary composition x = 0.20. X-ray total scattering shows suppression of diffuse scattering with increasing electric-field amplitude, indicative of an increase in structural ordering. Analysis of PDF peaks inmore » the 3–4-Å range shows ordering of Bi-Ti distances parallel to the applied electric field, illustrated by peak amplitude redistribution parallel and perpendicular to the electric-field vector. A transition from < 110 > to < 112 >-type off-center displacements of Bi relative to the neighboring Ti atoms is observable with increasing x. Analysis of PDF peak shift with electric field shows the effects of Bi-Ti redistribution and onset of piezoelectric lattice strain. Furthermore, the combination of these field-induced ordering mechanisms is consistent with local redistribution of Bi-Ti distances associated with domain reorientation and an overall increase in order of atomic displacements.« less

  19. A Rutile Chevron Modulation in Delafossite-Like Ga 3–x In 3 Ti x O 9+x/2

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rickert, Karl; Boullay, Philippe; Malo, Sylvie

    2016-05-02

    The structure solution of the modulated, delafossite-related, orthorhombic Ga 3–xIn 3Ti xO 9+x/2 for x = 1.5 is reported here in conjunction with a model describing the modulation as a function of x for the entire system. Previously reported structures in the related A 3–xIn 3Ti xO 9+x/2 (A = Al, Cr, or Fe) systems use X-ray diffraction to determine that the anion lattice is the source of modulation. Neutron diffraction, with its enhanced sensitivity to light atoms, offers a route to solving the modulation and is used here, in combination with precession electron diffraction tomography (PEDT), to solve themore » structure of Ga 1.5In 3Ti 1.5O 9.75. We construct a model that describes the anion modulation through the formation of rutile chevrons as a function of x. This model accommodates the orthorhombic phase (1.5 ≤ x ≤ 2.1) in the Ga 3-xIn 3Ti xO 9+x/2 system, which transitions to a biphasic mixture (2.2 ≤ x ≤ 2.3) with a monoclinic, delafossite-related phase (2.4 ≤ x ≤ 2.5). The optical band gaps of this system are determined, and are stable at ~3.4 eV before a ~0.4 eV decrease between x = 1.9 and 2.0. After this decrease, stability resumes at ~3.0 eV. Resistance to oxidation and reduction is also presented.« less

  20. Epilepsy caused by an abnormal alternative splicing with dosage effect of the SV2A gene in a chicken model.

    PubMed

    Douaud, Marine; Feve, Katia; Pituello, Fabienne; Gourichon, David; Boitard, Simon; Leguern, Eric; Coquerelle, Gérard; Vieaud, Agathe; Batini, Cesira; Naquet, Robert; Vignal, Alain; Tixier-Boichard, Michèle; Pitel, Frédérique

    2011-01-01

    Photosensitive reflex epilepsy is caused by the combination of an individual's enhanced sensitivity with relevant light stimuli, such as stroboscopic lights or video games. This is the most common reflex epilepsy in humans; it is characterized by the photoparoxysmal response, which is an abnormal electroencephalographic reaction, and seizures triggered by intermittent light stimulation. Here, by using genetic mapping, sequencing and functional analyses, we report that a mutation in the acceptor site of the second intron of SV2A (the gene encoding synaptic vesicle glycoprotein 2A) is causing photosensitive reflex epilepsy in a unique vertebrate model, the Fepi chicken strain, a spontaneous model where the neurological disorder is inherited as an autosomal recessive mutation. This mutation causes an aberrant splicing event and significantly reduces the level of SV2A mRNA in homozygous carriers. Levetiracetam, a second generation antiepileptic drug, is known to bind SV2A, and SV2A knock-out mice develop seizures soon after birth and usually die within three weeks. The Fepi chicken survives to adulthood and responds to levetiracetam, suggesting that the low-level expression of SV2A in these animals is sufficient to allow survival, but does not protect against seizures. Thus, the Fepi chicken model shows that the role of the SV2A pathway in the brain is conserved between birds and mammals, in spite of a large phylogenetic distance. The Fepi model appears particularly useful for further studies of physiopathology of reflex epilepsy, in comparison with induced models of epilepsy in rodents. Consequently, SV2A is a very attractive candidate gene for analysis in the context of both mono- and polygenic generalized epilepsies in humans.

  1. 23 CFR 627.5 - General principles and procedures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS VALUE ENGINEERING § 627.5 General principles and procedures. (a) State VE programs. State transportation... studies. (2) Studies. Value engineering studies shall follow the widely recognized systematic problem...

  2. 23 CFR 627.5 - General principles and procedures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS VALUE ENGINEERING § 627.5 General principles and procedures. (a) State VE programs. State transportation... studies. (2) Studies. Value engineering studies shall follow the widely recognized systematic problem...

  3. 23 CFR 627.5 - General principles and procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION ENGINEERING AND TRAFFIC OPERATIONS VALUE ENGINEERING § 627.5 General principles and procedures. (a) State VE programs. State transportation... studies. (2) Studies. Value engineering studies shall follow the widely recognized systematic problem...

  4. X-Ray Spectroscopic Laboratory Experiments In Support of the NASA X-Ray Astronomy Flight Program

    NASA Technical Reports Server (NTRS)

    Kahn, Steven M.; Savin, D. W.; Gu, M. F.; Beiersdorfer, P.; Liedahl, D. A.; Brown, G.; Utter, S.

    1998-01-01

    During the 1997 performance period, our work focused on the L-shell X-ray emission from highly charged iron ions in the 10-18 A region. Details of our accomplishments in 1997 are presented in the following. We start by describing the laboratory measurements made and their impact on the X-ray flight program and conclude by an overview of new instrumental capabilities developed for uses in the coming year.

  5. Modulation of the conformational state of the SV2A protein by an allosteric mechanism as evidenced by ligand binding assays

    PubMed Central

    Daniels, V; Wood, M; Leclercq, K; Kaminski, R M; Gillard, M

    2013-01-01

    Background and Purpose Synaptic vesicle protein 2A (SV2A) is the specific binding site of the anti-epileptic drug levetiracetam (LEV) and its higher affinity analogue UCB30889. Moreover, the protein has been well validated as a target for anticonvulsant therapy. Here, we report the identification of UCB1244283 acting as a SV2A positive allosteric modulator of UCB30889. Experimental Approach UCB1244283 was characterized in vitro using radioligand binding assays with [3H]UCB30889 on recombinant SV2A expressed in HEK cells and on rat cortex. In vivo, the compound was tested in sound-sensitive mice. Key Results Saturation binding experiments in the presence of UCB1244283 demonstrated a fivefold increase in the affinity of [3H]UCB30889 for human recombinant SV2A, combined with a twofold increase of the total number of binding sites. Similar results were obtained on rat cortex. In competition binding experiments, UCB1244283 potentiated the affinity of UCB30889 while the affinity of LEV remained unchanged. UCB1244283 significantly slowed down both the association and dissociation kinetics of [3H]UCB30889. Following i.c.v. administration in sound-sensitive mice, UCB1244283 showed a clear protective effect against both tonic and clonic convulsions. Conclusions and Implications These results indicate that UCB1244283 can modulate the conformation of SV2A, thereby inducing a higher affinity state for UCB30889. Our results also suggest that the conformation of SV2A per se might be an important determinant of its functioning, especially during epileptic seizures. Therefore, agents that act on the conformation of SV2A might hold great potential in the search for new SV2A-based anticonvulsant therapies. PMID:23530581

  6. Reliability of power profiles measured on NIMO TR1504 (Lambda-X) and effects of lens decentration for single vision, bifocal and multifocal contact lenses.

    PubMed

    Kim, Eon; Bakaraju, Ravi C; Ehrmann, Klaus

    2016-01-01

    To evaluate the repeatability of power profiles measured on NIMO TR1504 (Lambda-X, Belgium) and investigate the effects of lens decentration on the power profiles for single vision (SV), bifocal (BF) and multifocal (MF) contact lenses. Accuracy of the sphere power was evaluated using single vision BK-7 calibration glass lenses of six minus and six plus powers. Three SV and four BF/MF contact lenses - three lenses each, were measured five times to calculate the coefficients of repeatability (COR) of the instrument. The COR was computed for each chord position, lens design, prescription power and operator. One lens from each type was measured with a deliberate decentration up to ±0.5mm in 0.1mm steps. For all lenses, the COR varied across different regions of the half-chord position. In general, SV lenses showed lower COR compared to the BF/MF group lenses. There were no noticeable trends of COR between prescription powers for SV and BF/MF lenses. The shape of the power profiles was not affected when lenses were deliberately decentered for all SV and PureVision MF lenses. However, for Acuvue BF lenses, the peak to trough amplitude of the power profiles flattened up to 1.00D. The COR across the half-chord of the optic zone diameter was mostly within clinical relevance except for the central 0.5mm half-chord position. COR were dependent on the lens type, whereby BF/MF group produced higher COR than SV lenses. The effects of deliberate decentration on the shape of power profiles were pronounced for lenses where the profiles had sharp transitions of power. Copyright © 2015 Spanish General Council of Optometry. Published by Elsevier Espana. All rights reserved.

  7. 49 CFR 23.61 - Can recipients use quotas or set-asides as part of their ACDBE programs?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false Can recipients use quotas or set-asides as part of their ACDBE programs? 23.61 Section 23.61 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS Goals, Good Faith Efforts, and...

  8. 49 CFR 23.61 - Can recipients use quotas or set-asides as part of their ACDBE programs?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false Can recipients use quotas or set-asides as part of their ACDBE programs? 23.61 Section 23.61 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS Goals, Good Faith Efforts, and...

  9. 49 CFR 23.61 - Can recipients use quotas or set-asides as part of their ACDBE programs?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 1 2014-10-01 2014-10-01 false Can recipients use quotas or set-asides as part of their ACDBE programs? 23.61 Section 23.61 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS Goals, Good Faith Efforts, and...

  10. 49 CFR 23.61 - Can recipients use quotas or set-asides as part of their ACDBE programs?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false Can recipients use quotas or set-asides as part of their ACDBE programs? 23.61 Section 23.61 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS Goals, Good Faith Efforts, and...

  11. 49 CFR 23.61 - Can recipients use quotas or set-asides as part of their ACDBE programs?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Can recipients use quotas or set-asides as part of their ACDBE programs? 23.61 Section 23.61 Transportation Office of the Secretary of Transportation PARTICIPATION OF DISADVANTAGED BUSINESS ENTERPRISE IN AIRPORT CONCESSIONS Goals, Good Faith Efforts, and...

  12. The suitability of 129SvEv mice for studying depressive-like behaviour: both males and females develop learned helplessness.

    PubMed

    Chourbaji, Sabine; Pfeiffer, Natascha; Dormann, Christof; Brandwein, Christiane; Fradley, Rosa; Sheardown, Malcolm; Gass, P

    2010-07-29

    Behavioural studies using transgenic techniques in mice usually require extensive backcrossing to a defined background strain, e.g. to C57BL/6. In this study we investigated whether backcrossing can be replaced by using the 129SvEv strain from which the embryonic stem cells are generally obtained for gene targeting strategies to analyze e.g. depression-like behaviour. For that purpose we subjected male and female 129SvEv mice to two frequently used depression tests and compared them with commonly used C57BL/6 mice. 129SvEv and C57BL/6 mice exhibited differing profiles with regard to locomotion and pain sensitivity. However, in the learned helplessness paradigm, a procedure, which represents a valid method to detect depressive-like behaviour, 129SvEv animals develop a similar level of helplessness as C57BL/6 mice. One great advantage of the 129SvEv animals though, is the fact that in this strain even females develop helplessness, which could not be produced in C57BL/6 mice. In the tail suspension test, both genders of 129SvEv exhibited more despair behaviour than C57BL/6 animals. We therefore suggest that this strain may be utilized in the establishment of new test procedures for affective diseases, since costly and time-consuming backcrossing can be prevented, depressive-like behaviour may be analyzed effectively, and gender-specific topics could be addressed in an adequate way. Copyright 2010 Elsevier B.V. All rights reserved.

  13. Simultaneous use of solution NMR and X-ray data in REFMAC5 for joint refinement/detection of structural differences.

    PubMed

    Rinaldelli, Mauro; Ravera, Enrico; Calderone, Vito; Parigi, Giacomo; Murshudov, Garib N; Luchinat, Claudio

    2014-04-01

    The program REFMAC5 from CCP4 was modified to allow the simultaneous use of X-ray crystallographic data and paramagnetic NMR data (pseudocontact shifts and self-orientation residual dipolar couplings) and/or diamagnetic residual dipolar couplings. Incorporation of these long-range NMR restraints in REFMAC5 can reveal differences between solid-state and solution conformations of molecules or, in their absence, can be used together with X-ray crystallographic data for structural refinement. Since NMR and X-ray data are complementary, when a single structure is consistent with both sets of data and still maintains reasonably `ideal' geometries, the reliability of the derived atomic model is expected to increase. The program was tested on five different proteins: the catalytic domain of matrix metalloproteinase 1, GB3, ubiquitin, free calmodulin and calmodulin complexed with a peptide. In some cases the joint refinement produced a single model consistent with both sets of observations, while in other cases it indicated, outside the experimental uncertainty, the presence of different protein conformations in solution and in the solid state.

  14. Effect of capsid confinement on the chromatin organization of the SV40 minichromosome

    PubMed Central

    Saper, Gadiel; Kler, Stanislav; Asor, Roi; Oppenheim, Ariella; Raviv, Uri; Harries, Daniel

    2013-01-01

    Using small-angle X-ray scattering, we determined the three-dimensional packing architecture of the minichromosome confined within the SV40 virus. In solution, the minichromosome, composed of closed circular dsDNA complexed in nucleosomes, was shown to be structurally similar to cellular chromatin. In contrast, we find a unique organization of the nanometrically encapsidated chromatin, whereby minichromosomal density is somewhat higher at the center of the capsid and decreases towards the walls. This organization is in excellent agreement with a coarse-grained computer model, accounting for tethered nucleosomal interactions under viral capsid confinement. With analogy to confined liquid crystals, but contrary to the solenoid structure of cellular chromatin, our simulations indicate that the nucleosomes within the capsid lack orientational order. Nucleosomes in the layer adjacent to the capsid wall, however, align with the boundary, thereby inducing a ‘molten droplet’ state of the chromatin. These findings indicate that nucleosomal interactions suffice to predict the genome organization in polyomavirus capsids and underscore the adaptable nature of the eukaryotic chromatin architecture to nanoscale confinement. PMID:23258701

  15. Effect of capsid confinement on the chromatin organization of the SV40 minichromosome.

    PubMed

    Saper, Gadiel; Kler, Stanislav; Asor, Roi; Oppenheim, Ariella; Raviv, Uri; Harries, Daniel

    2013-02-01

    Using small-angle X-ray scattering, we determined the three-dimensional packing architecture of the minichromosome confined within the SV40 virus. In solution, the minichromosome, composed of closed circular dsDNA complexed in nucleosomes, was shown to be structurally similar to cellular chromatin. In contrast, we find a unique organization of the nanometrically encapsidated chromatin, whereby minichromosomal density is somewhat higher at the center of the capsid and decreases towards the walls. This organization is in excellent agreement with a coarse-grained computer model, accounting for tethered nucleosomal interactions under viral capsid confinement. With analogy to confined liquid crystals, but contrary to the solenoid structure of cellular chromatin, our simulations indicate that the nucleosomes within the capsid lack orientational order. Nucleosomes in the layer adjacent to the capsid wall, however, align with the boundary, thereby inducing a 'molten droplet' state of the chromatin. These findings indicate that nucleosomal interactions suffice to predict the genome organization in polyomavirus capsids and underscore the adaptable nature of the eukaryotic chromatin architecture to nanoscale confinement.

  16. Pesticide Program Dialogue Committee Meeting May 2-3, 2018

    EPA Pesticide Factsheets

    EPA’s Office of Pesticide Programs will hold a public meeting of the Pesticide Program Dialogue Committee (PPDC) on Wednesday, May 2, 2018, from 9:00 a.m. to 5:00 p.m., and on Thursday, May 3, from 9:00 a.m. to noon.

  17. Targeted Analysis of KLF6/KLF6-SV1 Regulating Pathways in Prostrate Cancer Development and Metastasis

    DTIC Science & Technology

    2010-09-01

    of KLF6 and KLF6-SV1 it will be important to define the functionality of the putative NLS, the 5BR, as well as the role of nucleo-cytoplasmic...presence of basic residues, Lys and Arg. In many cases 18 these signals are located near or within other important domains that regulate protein...transporter protein Crm1/Xpo1, first discovered in yeast (33-36). Subcellular localization and protein turnover are two related events that are tightly

  18. Exploring the Interaction of SV2A with Racetams Using Homology Modelling, Molecular Dynamics and Site-Directed Mutagenesis

    PubMed Central

    Lee, Joanna; Daniels, Veronique; Sands, Zara A.; Lebon, Florence; Shi, Jiye; Biggin, Philip C.

    2015-01-01

    The putative Major Facilitator Superfamily (MFS) transporter, SV2A, is the target for levetiracetam (LEV), which is a successful anti-epileptic drug. Furthermore, SV2A knock out mice display a severe seizure phenotype and die after a few weeks. Despite this, the mode of action of LEV is not known at the molecular level. It would be extremely desirable to understand this more fully in order to aid the design of improved anti-epileptic compounds. Since there is no structure for SV2A, homology modelling can provide insight into the ligand-binding site. However, it is not a trivial process to build such models, since SV2A has low sequence identity to those MFS transporters whose structures are known. A further level of complexity is added by the fact that it is not known which conformational state of the receptor LEV binds to, as multiple conformational states have been inferred by tomography and ligand binding assays or indeed, if binding is exclusive to a single state. Here, we explore models of both the inward and outward facing conformational states of SV2A (according to the alternating access mechanism for MFS transporters). We use a sequence conservation analysis to help guide the homology modelling process and generate the models, which we assess further with Molecular Dynamics (MD). By comparing the MD results in conjunction with docking and simulation of a LEV-analogue used in radioligand binding assays, we were able to suggest further residues that line the binding pocket. These were confirmed experimentally. In particular, mutation of D670 leads to a complete loss of binding. The results shed light on the way LEV analogues may interact with SV2A and may help with the on-going design of improved anti-epileptic compounds. PMID:25692762

  19. 23 CFR 658.5 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the highways, including military equipment, farm equipment, implements of husbandry, road construction... Title 23, U.S.C. For the purpose of this regulation this system includes toll roads designated as... materials; and military vehicles transporting marked military equipment or materiel. Over-the-road bus. The...

  20. NASA's X2000 Program: An Institutional Approach to Enabling Smaller Spacecraft

    NASA Technical Reports Server (NTRS)

    Deutsch, Leslie J.; Salvo, Chris; Woerner, David

    2000-01-01

    The number of NASA science missions per year is increasing from less than one to more than six. At the same time, individual mission budgets are smaller and cannot afford their own dedicated technology developments. In response to this, NASA has formed the X2000 Program. This program, which is divided into a set of subsequent "deliveries" will provide the basic avionics, power, communications, and software capability for future science missions. X2000 First Delivery, which will be completed in early 2001, will provide a full-functioned one MRAD tolerant flight computer, power switching electronics, a highly efficient radioisotope power source, and a transponder that provides high-level services at both 8.4 GHz and 32 GHz bands. The X2000 Second Delivery, which will be completed in the 2003 time frame, will enable complete spacecraft in the 10-50 kg class. All capabilities delivered by the X2000 program will be commercialized within the US and therefore will be available for others to use. Although the immediate customers for these technologies are deep space missions, most of the capabilities being delivered are generic in nature and will be equally applicable to Earth Observation missions.

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