yellow-phase american eels: Topics by Science.gov

Sample records for yellow-phase american eels

Environmental correlates of upstream migration of yellow-phase American eels in the Potomac River drainage

USGS Publications Warehouse

Welsh, Stuart A.; Heather L. Liller,

2013-01-01

Assessing the relationships between upstream migration and environmental variables is important to understanding the ecology of yellow-phase American Eels Anguilla rostrata. During an American Eel migration study within the lower Shenandoah River (Potomac River drainage), we counted and measured American Eels at the Millville Dam eel ladder for three periods: 14 May–23 July 2004, 7–30 September 2004, and 1 June–31 July 2005. Using generalized estimating equations, we modeled each time series of daily American Eel counts by fitting time-varying environmental covariates of lunar illumination (LI), river discharge (RD), and water temperature (WT), including 1-d and 2-d lags of each covariate. Information-theoretic approaches were used for model selection and inference. A total of 4,847 American Eels (19–74 cm total length) used the ladder during the three periods, including 2,622 individuals during a 2-d span following a hurricane-induced peak in river discharge. Additive-effects models of RD + WT, a 2-d lag of LI + RD, and LI + RD were supported for the three periods, respectively. Parameter estimates were positive for river discharge for each time period, negative for lunar illumination for two periods and positive for water temperature during one period. Additive-effects models supported synergistic influences of environmental variables on the upstream migration of yellow-phase American Eels, although river discharge was consistently supported as an influential correlate of upstream migration.
Diel periodicity and chronology of upstream migration in yellow-phase American eels (Anguilla rostrata)

USGS Publications Warehouse

Aldinger, Joni L.; Welsh, Stuart A.

2017-01-01

Yellow-phase American eel (Anguilla rostrata) upstream migration is temporally punctuated, yet migration chronology within diel time periods is not well-understood. This study examined diel periodicity, chronology, and total length (TL) of six multi-day, high-count (285–1,868 eels) passage events of upstream migrant yellow-phase American eels at the Millville Dam eel ladder, lower Shenandoah River, West Virginia during 2011–2014. We categorized passage by diel periods (vespertine, nocturnal, matutinal, diurnal) and season (spring, summer, late summer/early fall, fall). We depicted passage counts as time-series histograms and used time-series spectral analysis (Fast Fourier Transformation) to identify cyclical patterns and diel periodicity of upstream migration. We created histograms to examine movement patterns within diel periods for each passage event and fit normal mixture models (2–9 mixtures) to describe multiple peaks of passage counts. Periodicity of movements for each passage event followed a 24-h activity cycle with mostly nocturnal movement. Multimodal models were supported by the data; most modes represented nocturnal movements, but modes at or near the transition between twilight and night were also common. We used mixed-model methodology to examine relationships among TL, diel period, and season. An additive-effects model of diel period + season was the best approximating model. A decreasing trend of mean TL occurred across diel movement periods, with the highest mean TL occurring during fall relative to similar mean values of TL for spring, summer, and late summer/early fall. This study increased our understanding of yellow-phase American eels by demonstrating the non-random nature of their upstream migration.
Prevalence of Anguillicoloides crassus and growth variation in migrant yellow-phase American eels of the upper Potomac River drainage.

PubMed

Zimmerman, Jennifer L; Welsh, Stuart A

2012-11-08

Prevalence of the non-native swim bladder nematode Anguillicoloides crassus has recently increased in American eels from estuaries of the North American Atlantic coast, but little is known about parasite prevalence or conditions of previous infection in upstream migrant eels within upper watersheds. This study is the first to confirm presence of A. crassus in the upper Potomac River watershed. We estimated A. crassus prevalence during 3 time periods: September to October 2006 (5/143 eels, 3.5%), August to October 2007 (0/49 eels), and June 2008 (0/50 eels). All eels were sampled from the Millville Dam eel ladder on the lower Shenandoah River, a Potomac River tributary located approximately 285 km upstream of Chesapeake Bay, USA. Of the 5 infected eels, parasite intensity was 1 for each eel, and mean intensity was also 1.0. A swim bladder degenerative index (SDI) was calculated for the 50 eels from the final sampling period, and 38% of those eels (19 of 50) showed signs of previous infection by A. crassus. We also aged 42 of the 50 eels (mean ± SE = 6.7 ± 0.29 yr, range 4 to 11 yr) from the final sampling period. Based on the range of possible SDI scores (0 to 6), severity of previously infected swim bladders was moderate (SDI = 1 or 2). Previously infected eels, however, had a lower length-at-age than that of uninfected eels. Female yellow-phase eels in upper watersheds develop into large highly fecund silver-phase adults; hence, a parasite-induced effect on growth of yellow-phase eels could ultimately reduce reproductive potential.
Synergistic and singular effects of river discharge and lunar illumination on dam passage of upstream migrant yellow-phase American eels

USGS Publications Warehouse

Welsh, Stuart A.; Aldinger, Joni L.; Braham, Melissa A.; Zimmerman, Jennifer L.

2016-01-01

Monitoring of dam passage can be useful for management and conservation assessments of American eel, particularly if passage counts can be examined over multiple years. During a 7-year study (2007–2013) of upstream migration of American eels within the lower Shenandoah River (Potomac River drainage), we counted and measured American eels at the Millville Dam eel pass, where annual study periods were determined by the timing of the eel pass installation during spring or summer and removal during fall. Daily American eel counts were analysed with negative binomial regression models, with and without a year (YR) effect, and with the following time-varying environmental covariates: river discharge of the Shenandoah River at Millville (RDM) and of the Potomac River at Point of Rocks, lunar illumination (LI), water temperature, and cloud cover. A total of 17 161 yellow-phase American eels used the pass during the seven annual periods, and length measurements were obtained from 9213 individuals (mean = 294 mm TL, s.e. = 0.49, range 183–594 mm). Data on passage counts of American eels supported an additive-effects model (YR + LI + RDM) where parameter estimates were positive for river discharge (β = 7.3, s.e. = 0.01) and negative for LI (β = −1.9, s.e. = 0.34). Interestingly, RDM and LI acted synergistically and singularly as correlates of upstream migration of American eels, but the highest daily counts and multiple-day passage events were associated with increased RDM. Annual installation of the eel pass during late spring or summer prevented an early spring assessment, a period with higher RDM relative to those values obtained during sampling periods. Because increases in river discharge are climatically controlled events, upstream migration events of American eels within the Potomac River drainage are likely linked to the influence of climate variability on flow regime.
A semi-automated method of monitoring dam passage of American Eels Anguilla rostrata

USGS Publications Warehouse

Welsh, Stuart A.; Aldinger, Joni L.

2014-01-01

Fish passage facilities at dams have become an important focus of fishery management in riverine systems. Given the personnel and travel costs associated with physical monitoring programs, automated or semi-automated systems are an attractive alternative for monitoring fish passage facilities. We designed and tested a semi-automated system for eel ladder monitoring at Millville Dam on the lower Shenandoah River, West Virginia. A motion-activated eel ladder camera (ELC) photographed each yellow-phase American Eel Anguilla rostrata that passed through the ladder. Digital images (with date and time stamps) of American Eels allowed for total daily counts and measurements of eel TL using photogrammetric methods with digital imaging software. We compared physical counts of American Eels with camera-based counts; TLs obtained with a measuring board were compared with TLs derived from photogrammetric methods. Data from the ELC were consistent with data obtained by physical methods, thus supporting the semi-automated camera system as a viable option for monitoring American Eel passage. Time stamps on digital images allowed for the documentation of eel passage time—data that were not obtainable from physical monitoring efforts. The ELC has application to eel ladder facilities but can also be used to monitor dam passage of other taxa, such as crayfishes, lampreys, and water snakes.
Anthropogenic impacts on American eel demographics in Hudson River tributaries, New York

USGS Publications Warehouse

Machut, L.S.; Limburg, K.E.; Schmidt, R.E.; Dittman, D.

2007-01-01

Populations of American eel Anguilla rostrata along the eastern coast of North America have declined drastically for largely unknown reasons. We examined the population dynamics of American eels in six tributaries of the Hudson River, New York, to quantify their distribution and the impacts of anthropogenic stressors. With up to 155 American eels per 100 m2, tributary densities are greater than those within the main stem of the Hudson River and are among the highest reported anywhere. The predominance of small American eels (<200 mm) and wide range of ages (from young-of-year glass eels to 24-year-old yellow eels) suggest that tributaries are an important nursery area for immature American eels. However, upstream of natural and artificial barriers, American eel densities were reduced by at least a factor of 10 and condition, as measured by mass, was significantly lower. Significantly lower American eel condition was also found with increasing riparian urbanization. Density-dependent growth limitations below barriers are suggested by increased growth rates above the first tributary barrier. We suggest that (1) tributaries are important habitat for the conservation of American eels and (2) mitigation of anthropogenic stressors is vital for complete utilization of available habitat and conservation of the species. ?? Copyright by the American Fisheries Society 2007.
Donor life stage influences juvenile American eel Anguilla rostrata attraction to conspecific chemical cues

USGS Publications Warehouse

Galbraith, Heather S.; Blakeslee, Carrie J.; Schmucker, Andrew K.; Johnson, Nicholas; Hansen, Michael J.; Li, Weiming

2017-01-01

The present study investigated the potential role of conspecific chemical cues in inland juvenile American eel Anguilla rostrata migrations by assessing glass eel and 1 year old elver affinities to elver washings, and elver affinity to adult yellow eel washings. In two-choice maze assays, glass eels were attracted to elver washings, but elvers were neither attracted to nor repulsed by multiple concentrations of elver washings or to yellow eel washings. These results suggest that A. rostrata responses to chemical cues may be life-stage dependent and that glass eels moving inland may use the odour of the previous year class as information to guide migration. The role of chemical cues and olfaction in eel migrations warrants further investigation as a potential restoration tool.
Glass-eel-stage American Eels respond to conspecific odor as a function of concentration

USGS Publications Warehouse

Schmucker, Andrew K.; Johnson, Nicholas; Galbraith, Heather S.; Li, Weiming

2016-01-01

The American Eel Anguilla rostrata has experienced staggering population declines in recent decades and is now the focus of restoration efforts. Studies have demonstrated that olfaction is critical to anguillid behavior and that glass eels (the life stage which migrates inland from saltwater to freshwater) are attracted to conspecific washings. We evaluated conspecific cueing as a potential mechanism for American Eel inland migration coordination by assessing (1) the affinity of glass eels to conspecific washings, (2) the concentration–response relationships, and (3) changes in responsiveness to washings during the glass eel-to-elver transition. In two-choice maze assays, glass eels were attracted to glass eel washings over a wide range of concentrations (0.20–0.40 g of glass eels·L−1·h−1), and a logarithmic function provided the best fit to the concentration–response relationship. When given a choice between two conspecific washings of higher and lower concentrations, the glass eels generally preferred the higher concentration. Responses to undiluted glass eel washings did not significantly differ among stage-3–7 glass eels, although stage-7 eels were not attracted to the washings, whereas the other stages were. Washing affinity remained similar over the course of several weeks. These results support aspects of the conspecific cueing hypothesis at the glass eel life stage under laboratory conditions, suggesting that conspecific cueing is an important component of migration coordination among juvenile American Eels and warrants additional study.
Diurnal feeding behavior of the American Eel Anguilla rostrata

USGS Publications Warehouse

Engman, Augustin C.; Fischer, Jesse R.; Kwak, Thomas J.; Walter, Michael J.

2017-01-01

Despite potential to structure ecosystem food webs through top-down effects, the trophic interactions of the American Eel Anguilla rostrata remain largely understudied. All previous research on the trophic ecology of American Eel in inland aquatic ecosystems has been conducted in temperate continental regions of the species' range. These studies have led to a paradigm that American Eel is a nocturnally active benthic predator, which most commonly consumes benthic invertebrates. Tropical island streams and rivers have habitats and communities that are distinct from temperate counterparts, but comprise a large portion of the adult habitat in the American Eel's range. We documented a previously undescribed diurnal feeding behavior by American Eel in a Caribbean river and demonstrate that this behavior, and a shift toward more frequent daytime feeding, is linked to periodic mass migrations of postlarvae of amphidromous fish taxa, including the Sicydiine goby Sicydiumspp. Our findings indicate that periodic mass migrations of amphidromous postlarvae could function as a potentially important food source for American Eel in tropical regions of its distribution, despite the intermittence of availability. Furthermore, this suggests that the American Eel plays an important role in the structure of tropical lotic food webs through top-down effects that are potentially augmented by instream barriers.
The giant mottled eel, Anguilla marmorata, uses blue-shifted rod photoreceptors during upstream migration.

PubMed

Wang, Feng-Yu; Fu, Wen-Chun; Wang, I-Li; Yan, Hong Young; Wang, Tzi-Yuan

2014-01-01

Catadromous fishes migrate between ocean and freshwater during particular phases of their life cycle. The dramatic environmental changes shape their physiological features, e.g. visual sensitivity, olfactory ability, and salinity tolerance. Anguilla marmorata, a catadromous eel, migrates upstream on dark nights, following the lunar cycle. Such behavior may be correlated with ontogenetic changes in sensory systems. Therefore, this study was designed to identify changes in spectral sensitivity and opsin gene expression of A. marmorata during upstream migration. Microspectrophotometry analysis revealed that the tropical eel possesses a duplex retina with rod and cone photoreceptors. The λmax of rod cells are 493, 489, and 489 nm in glass, yellow, and wild eels, while those of cone cells are 508, and 517 nm in yellow, and wild eels, respectively. Unlike European and American eels, Asian eels exhibited a blue-shifted pattern of rod photoreceptors during upstream migration. Quantitative gene expression analyses of four cloned opsin genes (Rh1f, Rh1d, Rh2, and SWS2) revealed that Rh1f expression is dominant at all three stages, while Rh1d is expressed only in older yellow eel. Furthermore, sequence comparison and protein modeling studies implied that a blue shift in Rh1d opsin may be induced by two known (N83, S292) and four putative (S124, V189, V286, I290) tuning sites adjacent to the retinal binding sites. Finally, expression of blue-shifted Rh1d opsin resulted in a spectral shift in rod photoreceptors. Our observations indicate that the giant mottled eel is color-blind, and its blue-shifted scotopic vision may influence its upstream migration behavior and habitat choice.
Passage of downstream migrant American eels through an airlift-assisted deep bypass

USGS Publications Warehouse

Haro, Alexander J.; Watten, Barnaby J.; Noreika, John

2016-01-01

Traditional downstream guidance and bypass facilities for anadromous fishes (i.e., surface bypasses, surface guidance structures, and behavioral barriers) have frequently been ineffective for anguillid eels. Because eels typically spend the majority of their time near the bottom in the vicinity of intake structures, deep bypass structures with entrances near the bottom hold promise for increased effectiveness, thereby aiding in the recovery of this important species. A new design of a deep bypass system that uses airlift technology (the Conte Airlift Bypass) to induce flow in a bypass pipe was tested in a simulated intake entrance environment under controlled laboratory conditions. Water velocities of 0.9–1.5 m s−1 could be generated at the bypass entrance (opening with 0.073 m2 area), with corresponding flows through the bypass pipe of 0.07–0.11 m3 s−1. Gas saturation and hydrostatic pressure within the bypass pipe did not vary appreciably from a control (no air) condition under tested airflows. Migratory silver-phase American eels (Anguilla rostrata) tested during dark conditions readily located, entered, and passed through the bypass; initial avoidance rates (eels approaching but not entering the bypass entrance) were lower at higher entrance velocities. Eels that investigated the bypass pipe entrance tended to enter headfirst, but those that then exited the pipe upstream did so more frequently at lower entrance velocities. Eels appeared to swim against the flow while being transported downstream through the pipe; median transit times through the bypass for each test velocity ranged from 5.8 to 12.2 s, with transit time decreasing with increasing entrance velocity. Eels did not show strong avoidance of the vertical section of the pipe which contained injected air. No mortality or injury of bypassed eels was observed, and individual eels repeatedly passed through the bypass at rates of up to 40 passes per hour, suggesting that individuals do not
Retention and effects of miniature transmitters in juvenile American eels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mueller, Robert P.; Janak, Jill; Liss, Stephanie A.

This study was conducted to assess the effects of a non-functional acoustic micro transmitter (tag) on survival, tag loss, and swimming ability of juvenile American eels (Anguilla rostrata). The transmitter was designed for implantation through a < 3 mm opening into the body cavity of anguilliform fishes without the need for sutures. American eels used for the swimming performance study were 113–175 mm (N = 120). There were two treatment groups (tagged and non-tagged), each treatment was split into six size groups of 10 mm for testing. Potential transmitter effects on swimming performance were examined by comparing critical swimming speedsmore » (Ucrit an index of prolonged swimming performance) for tagged and non-tagged eels. There was no significant difference in Ucrit between tagged and non-tagged eels for all of the size groups tested. Median Ucrits for tagged eels ranged from 50.2 cm/s for the smallest group tested (113–119 mm) to 63.9 cm/s for fish 141–150 mm in length. Non-tagged group median Ucrits ranged from 47.2 cm/s for the smallest group to 66.9 cm/s for the 141–150 mm group. An additional 26 eels (115–208 mm) were tagged and held for 38 d (without undergoing swimming performance tests) to determine the effects on survival and tag loss. There was no mortality during the holding period and the majority of the tag loss occurred after 20 days post-tagging, which is the current projected life of the tag. Our results indicate that micro acoustic tags can be successfully implanted in juvenile American eels with no apparent impacts to swimming ability, and would be a viable option for examining eel movement patterns in river systems and near hydroelectric facilities.« less
Reproduction of European eel jeopardised by high levels of dioxins and dioxin-like PCBs?

PubMed

Geeraerts, C; Focant, J-F; Eppe, G; De Pauw, E; Belpaire, C

2011-09-01

Dioxins, furans and dioxin-like polychlorinated biphenyls (PCBs) were analysed in muscle tissue from yellow phased European eel (Anguilla anguilla) from 38 sites in Belgium. Dioxin concentrations in eel vary considerably between sampling locations, indicating that yellow eel is a good indicator of local pollution levels. Measured levels of dioxin-like PCBs are much higher than those of the dioxins and furans. In the majority of the sites, eel has levels considered to be detrimental for their reproduction. Field levels of dioxin and dioxin-like PCBs are therefore suggested as an additional causal factor contributing to the decline of the European eel. 42% of the sampling sites show especially dioxin-like PCB levels exceeding the European consumption level (with a factor 3 on average). Human consumption of eel, especially in these highly contaminated sites, seems unjustified. Copyright © 2011 Elsevier B.V. All rights reserved.
Gemfibrozil modulates cytochrome P450 and peroxisome proliferation-inducible enzymes in the liver of the yellow European eel (Anguilla anguilla).

PubMed

Lyssimachou, Angeliki; Thibaut, Rémi; Gisbert, Enric; Porte, Cinta

2014-01-01

The human lipid regulator gemfibrozil (GEM) has been shown to induce peroxisome proliferation in rodents leading to hepatocarcinogenesis. Since GEM is found at biological active concentrations in the aquatic environment, the present study investigates the effects of this drug on the yellow European eel (Anguilla anguilla). Eels were injected with different concentrations of GEM (0.1 to 200 μg/g) and sampled 24- and 96-h post-injection. GEM was shown to inhibit CYP1A, CYP3A and CYP2K-like catalytic activities 24-h post-injection, but at 96-h post-injection, only CYP1A was significantly altered in fish injected with the highest GEM dose. On the contrary, GEM had little effect on the phase II enzymes examined (UDP-glucuronyltransferase and glutathione-S-transferase). Peroxisome proliferation inducible enzymes (liver peroxisomal acyl-CoA oxidase and catalase) were very weakly induced. No evidence of a significant effect on the endocrine system of eels was observed in terms of plasmatic steroid levels or testosterone esterification in the liver.
Proceedings of a workshop on American Eel passage technologies

USGS Publications Warehouse

Haro, Alexander J.

2013-01-01

Recent concerns regarding a decline in recruitment of American eels (Anguilla rostrata) have prompted efforts to restore this species to historic habitats by providing passage for both upstream migrant juveniles and downstream migrant adults at riverine barriers, including low-head and hydroelectric dams (Castonguay et al. 1994, Haro et al. 2000). These efforts include development of management plans and stock assessment reviews in both the US and Canada (COSEWIC 2006, Canadian Eel Working Group 2009, DFO 2010, MacGregor et al. 2010, ASMFC 2000, ASMFC 2006, ASMFC 2008, Williams and Threader 2007), which target improvement of upstream and downstream passage for eels, as well as identification and prioritization of research needs for development of new and more effective passage technologies for American eels. Traditional upstream fish passage structures, such as fishways and fish lifts, are often ineffective passing juvenile eels, and specialized passage structures for this species are needed. Although designs for such passage structures are available and diverse (Knights and White 1998, Porcher 2002, FAO/DVWK 2002, Solomon and Beach 2004a,b, Environment Agency UK 2011), many biologists, managers, and engineers are unfamiliar with eel pass design and operation, or unaware of the technical options available for upstream eel passage, Better coordination is needed to account for eel passage requirements during restoration efforts for other diadromous fish species. Also, appropriately siting eel passes at hydropower projects is critical, and siting can be difficult and complex due to physical restrictions in access to points of natural concentrations of eels, dynamic hydraulics of tailrace areas, and presence of significant competing flows from turbine outfalls or spill. As a result, some constructed eel passes are sited poorly and may pass only a fraction of the number of eels attempting to pass the barrier. When sited and constructed appropriately, however, eel passes
Dam removal increases American eel abundance in distant headwater streams

USGS Publications Warehouse

Hitt, Nathaniel P.; Eyler, Sheila; Wofford, John E.B.

2012-01-01

American eel Anguilla rostrata abundances have undergone significant declines over the last 50 years, and migration barriers have been recognized as a contributing cause. We evaluated eel abundances in headwater streams of Shenandoah National Park, Virginia, to compare sites before and after the removal of a large downstream dam in 2004 (Embrey Dam, Rappahannock River). Eel abundances in headwater streams increased significantly after the removal of Embrey Dam. Observed eel abundances after dam removal exceeded predictions derived from autoregressive models parameterized with data prior to dam removal. Mann–Kendall analyses also revealed consistent increases in eel abundances from 2004 to 2010 but inconsistent temporal trends before dam removal. Increasing eel numbers could not be attributed to changes in local physical habitat (i.e., mean stream depth or substrate size) or regional population dynamics (i.e., abundances in Maryland streams or Virginia estuaries). Dam removal was associated with decreasing minimum eel lengths in headwater streams, suggesting that the dam previously impeded migration of many small-bodied individuals (<300 mm TL). We hypothesize that restoring connectivity to headwater streams could increase eel population growth rates by increasing female eel numbers and fecundity. This study demonstrated that dams may influence eel abundances in headwater streams up to 150 river kilometers distant, and that dam removal may provide benefits for eel management and conservation at the landscape scale.
Distribution and abundance of American eels in the White Oak River estuary, North Carolina

USGS Publications Warehouse

Hightower, J.E.; Nesnow, C.

2006-01-01

Apparent widespread declines in abundance of Anguilla rostrata (American eel) have reinforced the need for information regarding its life history and status. We used commercial eel pots and crab (peeler) pots to examine the distribution, condition, and abundance of American eels within the White Oak River estuary, NC, during summers of 2002-2003. Catch of American eels per overnight set was 0.35 (SE = 0.045) in 2002 and 0.49 (SE = 0.044) in 2003. There was not a significant linear relationship between catch per set and depth in 2002 (P = 0.31, depth range 0.9-3.4 m) or 2003 (P = 0.18, depth range 0.6-3.4 m). American eels from the White Oak River were in good condition, based on the slope of a length-weight relationship (3.41) compared to the median slope (3.15) from other systems. Estimates of population density from grid sampling in 2003 (300 mm and larger: 4.0-13.8 per ha) were similar to estimates for the Hudson River estuary, but substantially less than estimates from other (smaller) systems including tidal creeks within estuaries. Density estimates from coastal waters can be used with harvest records to examine whether overfishing has contributed to the recent apparent declines in American eel abundance.
Brominated flame retardants and Dechloranes in European and American eels from glass to silver life stages.

PubMed

Sühring, Roxana; Byer, Jonathan; Freese, Marko; Pohlmann, Jan-Dag; Wolschke, Hendrik; Möller, Axel; Hodson, Peter V; Alaee, Mehran; Hanel, Reinhold; Ebinghaus, Ralf

2014-12-01

The populations of American (Anguilla rostrata) and European eels (Anguilla anguilla) have been declining rapidly in the last decades. Organic contaminants are suspected to be one of the possible causes for the decline; however, so far there have been few investigations of the uptake of specific compounds by different life cycle stages (e.g. freshwater or marine stage) and how the contamination patterns develop throughout the eel's life cycle. In the present study we measured concentrations of polybrominated diphenylethers (PBDEs), alternate brominated flame retardants (alternate BFRs) and Dechloranes (Decs) in different life stages of European and American eels to compare the contamination patterns and their development throughout the eel's life cycle. In general, concentrations of flame retardants (FRs) were similar to or higher in American than in European eels, and a greater number of FRs were detected. PBDE congeners that are characteristic of the Penta-PBDE formulation were the most abundant FRs in all adult eels as well as American glass eels. In European glass eels the alternate BFR 2,3-dibromopropyl-2,4,6-tribromophenylether (DPTE) and Dechlorane Plus were the dominating FRs, with average concentrations of 1.1±0.31 ng g(-1) ww and up to 0.32 ng g(-1) ww respectively. Of the PBDEs BDE-183 was the most abundant congener in European glass eels. Low concentrations (less than 10% of the total contamination) of Tetra and Penta-PBDEs in juvenile European eels indicated that bans of technical Penta-PBDE in the European Union are effective. Enrichment of PBDEs was observed over the life stages of both European and American eels. However, a greater relative contribution of PBDEs to the sum FR contamination in American eels indicated an on-going exposure to these substances. High contributions of alternate BFRs in juvenile eels indicated an increased use of these substances in recent years. Concentrations seemed to be driven primarily by location, rather than life
Direct observations of American eels migrating across the continental shelf to the Sargasso Sea

PubMed Central

Béguer-Pon, Mélanie; Castonguay, Martin; Shan, Shiliang; Benchetrit, José; Dodson, Julian J.

2015-01-01

Since inferring spawning areas from larval distributions in the Sargasso Sea a century ago, the oceanic migration of adult American eels has remained a mystery. No adult eel has ever been observed migrating in the open ocean or in the spawning area. Here, we track movements of maturing eels equipped with pop-up satellite archival tags from the Scotian Shelf (Canada) into the open ocean, with one individual migrating 2,400 km to the northern limit of the spawning site in the Sargasso Sea. The reconstructed routes suggest a migration in two phases: one over the continental shelf and along its edge in shallow waters; the second in deeper waters straight south towards the spawning area. This study is the first direct evidence of adult Anguilla migrating to the Sargasso Sea and represents an important step forward in the understanding of routes and migratory cues. PMID:26505325
Draft genome of the American Eel (Anguilla rostrata).

PubMed

Pavey, Scott A; Laporte, Martin; Normandeau, Eric; Gaudin, Jérémy; Letourneau, Louis; Boisvert, Sébastien; Corbeil, Jacques; Audet, Céline; Bernatchez, Louis

2017-07-01

Freshwater eels (Anguilla sp.) have large economic, cultural, ecological and aesthetic importance worldwide, but they suffered more than 90% decline in global stocks over the past few decades. Proper genetic resources, such as sequenced, assembled and annotated genomes, are essential to help plan sustainable recoveries by identifying physiological, biochemical and genetic mechanisms that caused the declines or that may lead to recoveries. Here, we present the first sequenced genome of the American eel. This genome contained 305 043 contigs (N50 = 7397) and 79 209 scaffolds (N50 = 86 641) for a total size of 1.41 Gb, which is in the middle of the range of previous estimations for this species. In addition, protein-coding regions, including introns and flanking regions, are very well represented in the genome, as 95.2% of the 458 core eukaryotic genes and 98.8% of the 248 ultra-conserved subset were represented in the assembly and a total of 26 564 genes were annotated for future functional genomics studies. We performed a candidate gene analysis to compare three genes among all three freshwater eel species and, congruent with the phylogenetic relationships, Japanese eel (A. japanica) exhibited the most divergence. Overall, the sequenced genome presented in this study is a crucial addition to the presently available genetic tools to help guide future conservation efforts of freshwater eels. © 2016 John Wiley & Sons Ltd.

Fall diel diet composition of American eel (Anguilla rostrata) in a tributary of the Hudson River, New York, USA

USGS Publications Warehouse

Waldt, Emily M.; Abbett, Ross; Johnson, James H.; Dittman, Dawn E.; McKenna, James E.

2013-01-01

American eel (Anguilla rostrata), a once common species, is now in decline throughout much of its native range in North America. There is little information on the role of American eel in river food webs. A better understanding of the diet and ecological role of American eel will help in the conservation of this important species. During autumn 2009, eel and aquatic invertebrate samples were collected from Hannacroix Creek, a tributary of the Hudson River, in Albany and Greene counties, New York, USA. Eel diet was analyzed by the eel size and time period (day or night). A high proportion of eel stomachs were empty (73%). Eel diets varied among size classes and day and night feeding periods (p = 0.001). Diet overlap was significant between small and medium eels caught both during the day (α = 0.71) and at night (α = 0.84). Nocturnal diet and nocturnal invertebrate samples were similar (α = 0.65), indicating a preference for bottom feeding during the night. Mayfly nymphs were the major prey consumed in each period by all size classes. Among eels that fed, night-feeding eels had the greatest stomach weight (as a percent of total body weight). The swim-bladder parasite, Anguillicoloides crassus, was also observed in eels of all size classes with nearly 50% afflicted.
An evaluation of silver-stage American Eel conspecific chemical cueing during outmigration

USGS Publications Warehouse

Schmucker, Andrew K.; Johnson, Nicholas S.; Galbraith, Heather S.; Li, Weiming

2017-01-01

American Eel Anguilla rostrata abundance has declined in recent decades, in part because sexually maturing, silver-stage adults, outmigrating from freshwater to oceanic spawning grounds, encounter migratory blockades or perish when passing through active hydroelectric turbines. To help improve downstream passage effectiveness and increase survival rates, the role of silver-stage American Eel conspecific chemical cueing during outmigration was investigated using a new type of bioassay. Inside a laboratory flume, downstream-swimming eels were exposed to both live (putative attractant) and dead (putative repellent) conspecific washings to determine whether their trajectory of downstream movement, level of activity, or time spent inside targeted areas of the arena changed after exposure. Silver eels were not attracted to or repulsed by either odor, as none of five scoring metrics indicated a behavioral response. Results did not support the hypothesis that conspecific chemical cueing is a mechanism for downstream migration coordination or danger avoidance; however responses may not have been readily apparent in this type of assay. Fisheries managers may opt to focus future research on more feasible restoration efforts using alternate experimental designs to remedy this ecological issue.
Shark predation on migrating adult American eels (Anguilla rostrata) in the Gulf of St. Lawrence.

PubMed

Béguer-Pon, Mélanie; Benchetrit, José; Castonguay, Martin; Aarestrup, Kim; Campana, Steven E; Stokesbury, Michael J W; Dodson, Julian J

2012-01-01

In an attempt to document the migratory pathways and the environmental conditions encountered by American eels during their oceanic migration to the Sargasso Sea, we tagged eight silver eels with miniature satellite pop-up tags during their migration from the St. Lawrence River in Québec, Canada. Surprisingly, of the seven tags that successfully transmitted archived data, six were ingested by warm-gutted predators, as observed by a sudden increase in water temperature. Gut temperatures were in the range of 20 to 25°C-too cold for marine mammals but within the range of endothermic fish. In order to identify the eel predators, we compared their vertical migratory behavior with those of satellite-tagged porbeagle shark and bluefin tuna, the only endothermic fishes occurring non-marginally in the Gulf of St. Lawrence. We accurately distinguished between tuna and shark by using the behavioral criteria generated by comparing the diving behavior of these two species with those of our unknown predators. Depth profile characteristics of most eel predators more closely resembled those of sharks than those of tuna. During the first days following tagging, all eels remained in surface waters and did not exhibit diel vertical migrations. Three eels were eaten at this time. Two eels exhibited inverse diel vertical migrations (at surface during the day) during several days prior to predation. Four eels were eaten during daytime, whereas the two night-predation events occurred at full moon. Although tagging itself may contribute to increasing the eel's susceptibility to predation, we discuss evidence suggesting that predation of silver-stage American eels by porbeagle sharks may represent a significant source of mortality inside the Gulf of St. Lawrence and raises the possibility that eels may represent a reliable, predictable food resource for porbeagle sharks.
Downstream passage and impact of turbine shutdowns on survival of silver American Eels at five hydroelectric dams on the Shenandoah River

USGS Publications Warehouse

Eyler, Sheila; Welsh, Stuart A.; Smith, David R.; Rockey, Mary

2016-01-01

Hydroelectric dams impact the downstream migrations of silver American Eels Anguilla rostrata via migratory delays and turbine mortality. A radiotelemetry study of American Eels was conducted to determine the impacts of five run-of-the-river hydroelectric dams located over a 195-km stretch of the Shenandoah River, Virginia–West Virginia, during fall 2007–summer 2010. Overall, 96 radio-tagged individuals (mean TL = 85.4 cm) migrated downstream past at least one dam during the study. Most American Eels passed dams relatively quickly; over half (57.9%) of the dam passage events occurred within 1 h of reaching a dam, and most (81.3%) occurred within 24 h of reaching the dam. Two-thirds of the dam passage events occurred via spill, and the remaining passage events were through turbines. Migratory delays at dams were shorter and American Eels were more likely to pass via spill over the dam during periods of high river discharge than during low river discharge. The extent of delay in migration did not differ between the passage routes (spill versus turbine). Twenty-eight American Eels suffered turbine-related mortality, which occurred at all five dams. Mortality rates for eels passing through turbines ranged from 15.8% to 40.7% at individual dams. Overall project-specific mortality rates (with all passage routes combined) ranged from 3.0% to 14.3%. To protect downstream-migrating American Eels, nighttime turbine shutdowns (1800–0600 hours) were implemented during September 15–December 15. Fifty percent of all downstream passage events in the study occurred during the turbine shutdown period. Implementation of the seasonal turbine shutdown period reduced cumulative mortality from 63.3% to 37.3% for American Eels passing all five dams. Modifying the turbine shutdown period to encompass more dates in the spring and linking the shutdowns to environmental conditions could provide greater protection to downstream-migrating American Eels.
Built to bite? Differences in cranial morphology and bite performance between narrow- and broad-headed European glass eels.

PubMed

De Meyer, Jens; Van Wassenbergh, Sam; Bouilliart, Mathias; Dhaene, Jelle; Adriaens, Dominique

2018-03-01

The presence of two phenotypes in a single species is a widespread phenomenon, also observed in European eel (Anguilla anguilla). This dimorphism has been related to dietary differences in the subadult elver and yellow eel stages, with broad-heads generally feeding on harder and/or larger-bodied prey items than narrow-heads. Nevertheless, both broad- and narrow-headed phenotypes can already be found among glass eels, the stage preceding the elver eel stage. As these glass eels are considered nonfeeding, we investigate here to what degree the observed variation in head width is reflected in variation in the musculoskeletal feeding system, as well as whether this reflects the same variation observed in the older, dimorphic yellow eels. Additionally, we investigate whether musculoskeletal differences between broad- and narrow-headed glass eels have implications on their feeding performance and could thus impact prey preference when eels start feeding. Therefore, we compared the cranial musculoskeletal system of five broad- and narrow-headed glass eels using 3D-reconstructions and simulated the glass eel's bite force using the data of the muscle reconstructions. We found that the variation in the musculoskeletal system of glass eels indeed reflects that of the yellow eels. Broader heads were related to larger jaw muscles, responsible for mouth closure. Accordingly, broad-heads could generate higher bite forces than narrow-headed glass eels. In addition, broader heads were associated with higher coronoid processes and shorter hyomandibulae, beneficial for dealing with higher mechanical loadings and consequently, harder prey. We, thus, show that head width variation in glass eels is related to musculoskeletal differences which, in turn, can affect feeding performance. As such, differences in prey preference can already take place the moment the eels start feeding, potentially leading to the dimorphism observed in the elver and yellow eel stage. © 2017 Wiley Periodicals
Behavior and passage of silver-phase American eels, Anguilla rostrata (LeSueur), at a small hydroelectric facility

USGS Publications Warehouse

Haro, Alex; Castro-Santos, Ted; Boubée, Jacques

2000-01-01

Downstream migrant eels were monitored near a small (51 MW) hydroelectric facility on the Connecticut River (Massachusetts, USA) for two seasons using acoustic and radio telemetry. Eels frequently made several attempts over periods of one to several days to pass the station. Did activity of eels was variable, although most movements occurred at night. Eels occupied a variety of depths in the forebay area, but spent the greater proportion of time at or near the bottom (10 m), occasionally venturing to the surface. Horizontal movements usually spanned across the entire width of the forebay. There was no significant relationship between duration of forebay presence and either flow or light intensity. Although all telemetered eels passed via the turbines, some migrant eels did use a surface bypass.
Variation in organotin accumulation in relation to the life history in the Japanese eel Anguilla japonica

NASA Astrophysics Data System (ADS)

Ohji, Madoka; Harino, Hiroya; Arai, Takaomi

2009-08-01

In order to examine the ecological risks caused by organotin compounds (OTs) in diadromous fish migrating between sea and freshwaters, tributyltin (TBT) and triphenyltin (TPT) compounds and their breakdown products were determined in the catadromous eel Anguilla japonica, which has sea, estuarine and river life histories, collected in Japanese sea, brackish and freshwaters within the same region. Ontogenic changes in otolith strontium (Sr) and calcium (Ca) concentrations were examined along the life history transect to discriminate the migration type. There were generally three different patterns, which were categorized as 'sea eels', 'estuarine eels' and 'river eels' according to the otolith Sr:Ca ratio. The concentrations of TBT in silver eels (mature eels) were significantly higher than that in yellow eels (immature eels), and the percentages of TBT were also higher in silver eels than in yellow eels. A positive correlation was found between TBT concentration and the gonad-somatic index (GSI). It is thus considered that silver eels have a higher risk of contamination by TBT than yellow eels. TBT and TPT concentrations in sea eels were significantly higher than those in river eels, while no significant differences were observed in TBT and TPT concentrations in estuarine eels compared to sea and river eels. These results suggest that sea eels have a higher ecological risk of OT contamination than river eels during their life history, and the risk of OTs in estuarine eels is considered to be intermediate between that of sea and river eels. Positive linear relationships were found between Sr:Ca ratios and the concentrations of TBT and TPT. Therefore, these results suggest that the ecological risk of OTs increase as the sea residence period in the eel becomes longer. TBT and TPT concentrations in sea eels were significantly higher than those in river eels even at the same growth stage. Thus, it is clear that migratory type is the most important factor for OT
Differences in organotin accumulation among ecological migratory types of the Japanese eel Anguilla japonica

NASA Astrophysics Data System (ADS)

Ohji, Madoka; Harino, Hiroya; Arai, Takaomi

2006-08-01

In order to examine the ecological risks caused by organotin compounds (OTs) in diadromous fish migrating between sea and freshwater, tributyltin (TBT) and triphenyltin (TPT) compounds, and their breakdown products, were determined in the catadromous eel Anguilla japonica having sea, estuarine and river life histories, collected in Japanese sea, brackish and fresh waters. Ontogenic changes in otolith strontium (Sr) and calcium (Ca) concentrations were examined along life history transect to discriminate the migration type. There were generally three different patterns, which were categorized 'sea eels' (spent most of their life in the sea and did not enter freshwater), 'estuarine eels' (inhabited estuaries or switched between different habitats), and 'river eels' (entered and remained in freshwater river habitats after arrival in the estuary) according to the otolith Sr:Ca ratio. There were generally no significant correlations between TBT and TPT accumulation and various biological characteristics such as total length (TL), body weight (BW), age and sex in A. japonica. The concentrations of TBT and TPT in silver eels (mature eels) were significantly higher than those in yellow eels (immature eels), and the percentages of TBT and TPT were also higher in silver eels than in yellow eels. A positive correlation was found between TBT concentration and the gonad-somatic index (GSI). It is thus considered that silver eels have a higher risk of contamination by TBT than yellow eels. TBT and TPT concentrations in sea eels were significantly higher than those in river eels. In contrast, no significant differences were observed in TBT and TPT concentrations in estuarine eels compared to sea and river eels. These results suggest that sea eels have a higher ecological risk of OT contamination than river eels during their life history, and the risk of OTs in estuarine eels is considered to be intermediate between that of sea and river eels. Positive linear relationships were
Gonadal transcriptome analysis of wild contaminated female European eels during artificial gonad maturation.

PubMed

Baillon, Lucie; Oses, Jennifer; Pierron, Fabien; Bureau du Colombier, Sarah; Caron, Antoine; Normandeau, Eric; Lambert, Patrick; Couture, Patrice; Labadie, Pierre; Budzinski, Hélène; Dufour, Sylvie; Bernatchez, Louis; Baudrimont, Magalie

2015-11-01

Since the early 1980s, the population of European eels (Anguilla anguilla) has dramatically declined. Nowadays, the European eel is listed on the red list of threatened species (IUCN Red List) and is considered as critically endangered of extinction. Pollution is one of the putative causes for the collapse of this species. Among their possible effects, contaminants gradually accumulated in eels during their somatic growth phase (yellow eel stage) would be remobilized during their reproductive migration leading to potential toxic events in gonads. The aim of this study was to investigate the effects of organic and inorganic contaminants on the gonad development of wild female silver eels. Female silver eels from two sites with differing contamination levels were artificially matured. Transcriptomic analyses by means of a 1000 candidate gene cDNA microarray were performed on gonads after 11weeks of maturation to get insight into the mechanisms of toxicity of contaminants. The transcription levels of several genes, that were associated to the gonadosomatic index (GSI), were involved in mitotic cell division but also in gametogenesis. Genes associated to contaminants were mainly involved in the mechanisms of protection against oxidative stress, in DNA repair, in the purinergic signaling pathway and in steroidogenesis, suggesting an impairment of gonad development in eels from the polluted site. This was in agreement with the fact that eels from the reference site showed a higher gonad growth in comparison to contaminated fish. Copyright © 2015 Elsevier Ltd. All rights reserved.
Head shape disparity impacts pollutant accumulation in European eel.

PubMed

De Meyer, Jens; Belpaire, Claude; Boeckx, Pascal; Bervoets, Lieven; Covaci, Adrian; Malarvannan, Govindan; De Kegel, Barbara; Adriaens, Dominique

2018-05-08

Several aspects of the life cycle of the critically endangered European eel (Anguilla anguilla) remain poorly understood. One such aspect is the broad-versus narrow-head dimorphism, and how this impacts their overall performance at different stages of their life cycle. At the yellow eel stage, the phenotypes show a trophic divergence. We investigated whether pollutant accumulation is affected by this disparity. We show that broad-headed eels contained higher concentrations of mercury and several lipophilic organic pollutants, compared to narrow-headed ones, irrespective of their fat content. The hereby confirmed link between the phenotypic disparity, its associated feeding ecology and its impact on pollutant accumulation thus raises further concerns about their migratory and reproductive success. Considering that pollution is an important contributor to the European eel's decline, our results demonstrate that broad-headed eels are more vulnerable to detrimental pollutant accumulation. This compromises their successful contribution to their population's reproduction and its restoration. Copyright © 2018 Elsevier Ltd. All rights reserved.
Acute toxicity of two lampricides, 3-trifluoromethyl-4-nitrophenol (TFM) and a TFM: 1% niclosamide mixture, to sea lamprey, three species of unionids, haliplid water beetles, and American eel

USGS Publications Warehouse

Boogaard, Michael A.; Rivera, Jane E.

2011-01-01

We conducted a series of toxicological treatments with 3-trifluoromethyl-4-nitrophenol (TFM) and a TFM:1% 2′,5-dichloro-4′-nitrosalicylanilide (niclosamide) mixture, two compounds used to control larval sea lamprey (Petromyzon marinus) in Great Lakes tributaries, to evaluate the acute toxicity of the lampricides to a number of nontarget species of concern. Treatments were conducted with yellow stage American eel (Anguilla rostrata), adult and larval haliplid water beetles (Haliplus spp.), a surrogate for the endangered Hungerford’s crawling water beetle (Brychius hungerfordi), and adults of three unionid species—giant floater (Pyganadon grandis), fragile papershell (Leptodea fragilis), and pink heelsplitter (Potamilus alatus). Treatments were conducted using a serial dilution system consisting of nine test concentrations and an untreated control with 20% dilution between concentrations. Narcosis was evident among giant floaters exposed to the TFM and the TFM:1% niclosamide mixture and among pink heelsplitters exposed to the TFM:1% niclosamide mixture only but mostly at concentrations greater than 2-fold that required to kill 100% of larval sea lamprey (minimum lethal concentration (MLC)). Tests with the haliplid beetle suggest the risks to the Hungerford’s crawling water beetle associated with TFM applications are minimal. Concentrations over 2-fold the sea lamprey MLC did not kill adult or larval water beetles. Preliminary behavioral observations suggest water beetles may avoid treatment by crawling out of the water. Adult water beetles exposed to TFM at 3-fold the sea lamprey MLC were observed above the water line more often than controls. The lampricide TFM was not acutely toxic to American eel. Mortalities were rare among American eel exposed to TFM concentrations up to 7-fold the observed sea lamprey MLC. Similarly, for the TFM:1% niclosamide mixture, mortalities were rare among American eel exposed to nearly 5-fold the observed sea lamprey MLC
Signatures of natural selection between life cycle stages separated by metamorphosis in European eel.

PubMed

Pujolar, J M; Jacobsen, M W; Bekkevold, D; Lobón-Cervià, J; Jónsson, B; Bernatchez, L; Hansen, M M

2015-08-13

Species showing complex life cycles provide excellent opportunities to study the genetic associations between life cycle stages, as selective pressures may differ before and after metamorphosis. The European eel presents a complex life cycle with two metamorphoses, a first metamorphosis from larvae into glass eels (juvenile stage) and a second metamorphosis into silver eels (adult stage). We tested the hypothesis that different genes and gene pathways will be under selection at different life stages when comparing the genetic associations between glass eels and silver eels. We used two sets of markers to test for selection: first, we genotyped individuals using a panel of 80 coding-gene single nucleotide polymorphisms (SNPs) developed in American eel; second, we investigated selection at the genome level using a total of 153,423 RAD-sequencing generated SNPs widely distributed across the genome. Using the RAD approach, outlier tests identified a total of 2413 (1.57%) potentially selected SNPs. Functional annotation analysis identified signal transduction pathways as the most over-represented group of genes, including MAPK/Erk signalling, calcium signalling and GnRH (gonadotropin-releasing hormone) signalling. Many of the over-represented pathways were related to growth, while others could result from the different conditions that eels inhabit during their life cycle. The observation of different genes and gene pathways under selection when comparing glass eels vs. silver eels supports the adaptive decoupling hypothesis for the benefits of metamorphosis. Partitioning the life cycle into discrete morphological phases may be overall beneficial since it allows the different life stages to respond independently to their unique selection pressures. This might translate into a more effective use of food and niche resources and/or performance of phase-specific tasks (e.g. feeding in the case of glass eels, migrating and reproducing in the case of silver eels).
The shocking predatory strike of the electric eel.

PubMed

Catania, Kenneth

2014-12-05

Electric eels can incapacitate prey with an electric discharge, but the mechanism of the eel's attack is unknown. Through a series of experiments, I show that eel high-voltage discharges can activate prey motor neurons, and hence muscles, allowing eels to remotely control their target. Eels prevent escape in free-swimming prey using high-frequency volleys to induce immobilizing whole-body muscle contraction (tetanus). Further, when prey are hidden, eels can emit periodic volleys of two or three discharges that cause massive involuntary twitch, revealing the prey's location and eliciting the full, tetanus-inducing volley. The temporal patterns of eel electrical discharges resemble motor neuron activity that induces fast muscle contraction, suggesting that eel high-voltage volleys have been selected to most efficiently induce involuntary muscle contraction in nearby animals. Copyright © 2014, American Association for the Advancement of Science.
STUDIES ON SOUTH AMERICAN YELLOW FEVER

PubMed Central

Davis, Nelson C.; Shannon, Raymond C.

1929-01-01

Yellow fever virus from M. rhesus has been inoculated into a South American monkey (Cebus macrocephalus) by blood injection and by bites of infected mosquitoes. The Cebus does not develop the clinical or pathological signs of yellow fever. Nevertheless, the virus persists in the Cebus for a time as shown by the typical symptoms and lesions which develop when the susceptible M. rhesus is inoculated from a Cebus by direct transfer of blood or by mosquito (A. aegypti) transmission. PMID:19869607
Imported Asian swamp eels (Synbranchidae: Monopterus) in North American live food markets: Potential vectors of non-native parasites

USGS Publications Warehouse

Nico, Leo G.; Sharp, Paul; Collins, Timothy M.

2011-01-01

Since the 1990s, possibly earlier, large numbers of Asian swamp eels (Synbranchidae: Monopterus spp.), some wild-caught, have been imported live from various countries in Asia and sold in ethnic food markets in cities throughout the USA and parts of Canada. Such markets are the likely introduction pathway of some, perhaps most, of the five known wild populations of Asian swamp eels present in the continental United States. This paper presents results of a pilot study intended to gather baseline data on the occurrence and abundance of internal macroparasites infecting swamp eels imported from Asia to North American retail food markets. These data are important in assessing the potential role that imported swamp eels may play as possible vectors of non-native parasites. Examination of the gastrointestinal tracts and associated tissues of 19 adult-sized swamp eels—identified as M. albus "Clade C"—imported from Vietnam and present in a U.S. retail food market revealed that 18 (95%) contained macroparasites. The 394 individual parasites recovered included a mix of nematodes, acanthocephalans, cestodes, digeneans, and pentastomes. The findings raise concern because of the likelihood that some parasites infecting market swamp eels imported from Asia are themselves Asian taxa, some possibly new to North America. The ecological risk is exacerbated because swamp eels sold in food markets are occasionally retained live by customers and a few reportedly released into the wild. For comparative purposes, M. albus "Clade C" swamp eels from a non-native population in Florida (USA) were also examined and most (84%) were found to be infected with internal macroparasites. The current level of analysis does not allow us to confirm whether these are non-native parasites.
A novel recombinant bivalent outer membrane protein of Vibrio vulnificus and Aeromonas hydrophila as a vaccine antigen of American eel (Anguilla rostrata).

PubMed

SongLin, Guo; PanPan, Lu; JianJun, Feng; JinPing, Zhao; Peng, Lin; LiHua, Duan

2015-04-01

The immogenicity of a novel vaccine antigen was evaluated after immunized American eels (Anguilla rostrata) with a recombinant bivalent expressed outer membrane protein (OMP) of Vibrio vulnificus and Aeromonas hydrophila. Three groups of eels were intraperitoneal (i.p) injected with phosphate-buffered saline (PBS group), formaline-killed-whole-cell (FKC) of A. hydrophila and V. vulnificus (FKC group) or the bivalent OMP (OMP group). On 14, 21, 28 and 42 days post-vaccination respectively, proliferation of the whole blood cells, titers of specific antibody and lysozyme activities of experimental eels were detected. On 28 day post-vaccination, eels from three groups were challenged by i.p injection of live A. hydrophila or V. vulnificus. The results showed that, compared with the PBS group, proliferation of whole blood cells in OMP group was significant enhanced on 28 days, and the serum titers of anti-A.hydrophila and anti-V. vulnificus antibody in eels of FKC and OMP group were significant increased on 14, 21 and 28d. Lysozyme Activities in serum, skin mucus, liver and kidney were significant changed between the three groups. Relative Percent Survival (RPS) after challenged A. hydrophila in KFC vs. PBS group and OMP vs. PBS group were 62.5% and 50% respectively, and the RPS challenged V. vulnificus in FKC and OMP vs. PBS group were 37.5% and 50% respectively. These results suggest that American eels immunized with the bivalent OMP would positively affect specific as well as non-specific immune parameters and protect against infection by the two pathogens in fresh water farming. Copyright © 2015 Elsevier Ltd. All rights reserved.
Community composition, diversity, and metabolism of intestinal microbiota in cultivated European eel (Anguilla anguilla).

PubMed

Huang, Wei; Cheng, Zhiqiang; Lei, Shaonan; Liu, Lanying; Lv, Xin; Chen, Lihua; Wu, Miaohong; Wang, Chao; Tian, Baoyu; Song, Yongkang

2018-05-01

The intestinal tract, which harbours tremendous numbers of bacteria, plays a pivotal role in the digestion and absorption of nutrients. Here, high-throughput sequencing technology was used to determine the community composition and complexity of the intestinal microbiota in cultivated European eels during three stages of their lifecycle, after which the metabolic potentials of their intestinal microbial communities were assessed. The results demonstrated that European eel intestinal microbiota were dominated by bacteria in the phyla Proteobacteria and Fusobacteria. Statistical analyses revealed that the three cultured European eel life stages (elver, yellow eel, and silver eel) shared core microbiota dominated by Aeromonas. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) predictions of metagenome function revealed that the European eel intestinal microbiota might play significant roles in host nutrient metabolism. Biolog AN MicroPlate™ analysis and extracellular enzyme assays of culturable intestinal bacteria showed that the intestinal microbiota have a marked advantage in the metabolism of starch, which is the main carbohydrate component in European eel formulated feed. Understanding the ecology and functions of the intestinal microbiota during different developmental stages will help us improve the effects of fish-based bacteria on the composition and metabolic capacity of nutrients in European eels.
Phthalate metabolites in the European eel (Anguilla anguilla) from Mediterranean coastal lagoons.

PubMed

Fourgous, C; Chevreuil, M; Alliot, F; Amilhat, E; Faliex, E; Paris-Palacios, S; Teil, M J; Goutte, A

2016-11-01

The levels and fate of phthalate metabolites have been poorly evaluated in fish, despite their potential ecotoxicological impacts. The present study aims to characterize the levels of phthalate metabolites in muscle tissue of yellow eels (Anguilla anguilla) from two coastal Mediterranean lagoons, during three sampling periods. Nine phthalate metabolites were detected in >70% of the samples. Slightly higher levels of phthalate metabolites were detected in March and June compared to October, suggesting possible seasonal variations in environmental release and/or phthalate metabolization process by eels. The large sample size (N=117) made it possible to explore correlations between phthalate metabolites' levels and individual parameters, such as body length, age, body condition and hepatic histo-pathologies. Body length and estimated age poorly correlated with phthalate metabolites, suggesting that eels did not accumulate phthalates during growth, contrary to persistent compounds. Eels presented different grades of hepatic fibrosis and lipidosis. A negative correlation was found between the severity of these pathologies in the liver and the sum of phthalate metabolites levels, supporting the hypothesis that eels with damaged liver are less able to metabolize xenobiotics. Copyright © 2016 Elsevier B.V. All rights reserved.
Low PCB concentrations observed in American eel (Anguilla rostrata) in six Hudson River tributaries

USGS Publications Warehouse

Limburg, K.E.; Machut, L.S.; Jeffers, P.; Schmidt, R.E.

2008-01-01

We analyzed 73 eels, collected in 2004 and 2005 above the head of tide in six Hudson River tributaries, for total PCBs, length, weight, age, and nitrogen stable isotope ratios (??15N). Mean total PCB concentration (wet weight basis) was 0.23 ppm ?? 0.08 (standard error), with a range of 0.008 to 5.4 ppm. A majority of eels (84) had concentrations below 0.25 ppm, and only seven eels (10%) had concentrations exceeding 0.5 ppm. Those eels with higher PCB concentrations were ???12 yr; there was a weak correlation of PCB concentration with ??15N and also with weight. Compared to recent (2003) data from the mainstem of the Hudson River estuary, these results indicate that tributaries are generally much less contaminated with PCBs. We hypothesize that those tributary eels with high PCB concentrations were relatively recent immigrants from the mainstem. Given concern over the possible adverse effects of PCBs on eel reproduction, these tributaries may serve as refugia. Therefore, providing improved access to upland tributaries may be critically important to this species. ?? 2008 Northeastern Naturalist.
Karyological and gonadal sex of eels (Anguilla anguilla) from the German Bight and the lower River Elbe

NASA Astrophysics Data System (ADS)

Passakas, T.; Tesch, F.-W.

1980-06-01

Yellow eels (Anguilla anguilla) taken during summer from random commercial trapnet samples in the littoral area of Helgoland (n=116) and from a freshwater area of the River Elbe near Hamburg (n=109) were examined with regard to their karyological (i.e. existence of female sex chromosomes) and gonadal sex. In 47 % and 21 % of the two samples, respectively, chromosomes were unidentifiable because of insufficient numbers of mitotic plates. All eels from Helgoland, except one phenotypically undetermined fish, exhibited female gonads: 48 had female sex chromosomes and 13 were karyologically males. As found previously in the River Elbe, eels with male gonads predominated (n=55); 25 were undifferentiated. Of the gonadal males 26 were karyological males and 16 karyological females; the rest could not be identified by chromosome patterns. In contrast, all but one of the Elbe eels with female gonads (n=28) had female sex chromosomes. Some aspects of the sex reversal documented in the eel are considered.

Do we protect freshwater eels or do we drive them to extinction?

PubMed

Arai, Takaomi

2014-01-01

Freshwater eels are important animals because they have a unique catadromous life history and are used as food resources. European, American and Japanese eel populations now are considered to be outside the safe biological limits and are seriously threatened with extinction. Therefore, the European eel was recently categorised as critically endangered by the European Union and the United Nations. One of the reasons for the drastic decline in eel populations is overfishing, which has caused a high demand for eel aquaculture; eel aquaculture completely depends on wild juveniles, and in contrast to animals, artificial propagation has not yet succeeded for the eels. Therefore, commercial eel industries are now considering tropical eels as possible replacement for European and Japanese eels to compensate for declining stocks. In this study, I attempt to examine the present status of the biology and stock of tropical eels. However, useful scientific research and information on the biology and stock assessments of tropical eels are lacking, a situation quite different from that for other temperate freshwater eels, which have been well studied for several decades with trends and recruitment patterns being on record. Nevertheless, the present tropical eel catch has been reported as being less than half that of 20 years ago. The present trends in eel stocks and utilization for human consumption suggest that all eel populations will decline to numbers that fall outside safe biological limits and will be seriously threatened with extinction without protection and conservation from strict enforcement of local and international laws.
Quantitative Phase Fraction Detection in Organic Photovoltaic Materials through EELS Imaging

DOE PAGES

Dyck, Ondrej; Hu, Sheng; Das, Sanjib; ...

2015-11-24

Organic photovoltaic materials have recently seen intense interest from the research community. Improvements in device performance are occurring at an impressive rate; however, visualization of the active layer phase separation still remains a challenge. Our paper outlines the application of two electron energy-loss spectroscopic (EELS) imaging techniques that can complement and enhance current phase detection techniques. Specifically, the bulk plasmon peak position, often used to produce contrast between phases in energy filtered transmission electron microscopy (EFTEM), is quantitatively mapped across a sample cross section. One complementary spectrum image capturing the carbon and sulfur core loss edges is compared with themore » plasmon peak map and found to agree quite well, indicating that carbon and sulfur density differences between the two phases also allows phase discrimination. Additionally, an analytical technique for determining absolute atomic areal density is used to produce an absolute carbon and sulfur areal density map. We also show how these maps may be re-interpreted as a phase ratio map, giving quantitative information about the purity of the phases within the junction.« less
Spatial trends of organochlorinated pesticides, polychlorinated biphenyls, and polybrominated diphenyl ethers in Atlantic Anguillid eels.

PubMed

Byer, Jonathan D; Lebeuf, Michel; Alaee, Mehran; Stephen, Brown R; Trottier, Steve; Backus, Sean; Keir, Michael; Couillard, Catherine M; Casselman, John; Hodson, Peter V

2013-02-01

The bioaccumulation of lipophilic contaminants such as polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and polybrominated diphenyl ethers (PBDEs) can result in a reduction in fitness and spawner quality in eels and may be a factor in Anguillid sp. population declines. Contaminant concentrations in eels have been studied extensively in Europe, but data for American eels are severely lacking. Concentrations of PCBs, OCPs, and PBDEs were determined in American eel from eastern Canada and New York, USA, along with European eel from Belgium. Principal component analysis revealed that eels captured in the St. Lawrence estuary were a mixture of upstream migrants from the St. Lawrence River watershed, and fish captured in local tributaries. Contaminant concentrations were dependent on origin, related to the local environment, and were lower than historic values. In Canada, concentrations of OCPs and PCBs in eel tissues were below the Canadian human consumption guidelines for contaminants in fish, indicating that the current risk to consumers is low. However, concentrations of PCBs, total DDT, and mirex in eels from L. Ontario and the upper St. Lawrence R. were above Great Lakes guidelines for the protection of piscivorous predators. Concentrations of penta-BDE homologs exceeded the Canadian guideline for environmental quality in over half of the eels in this study, but concentrations of the other homolog groups were below the guideline. Copyright © 2012 Elsevier Ltd. All rights reserved.
Dimorphism throughout the European eel's life cycle: are ontogenetic changes in head shape related to dietary differences?

PubMed

De Meyer, J; Goethals, T; Van Wassenbergh, S; Augustijns, T; Habraken, J; Hellemans, J; Vandewiele, V; Dhaene, J; Bouillart, M; Adriaens, D

2018-05-31

A well-known link exists between an organism's ecology and morphology. In the European eel, a dimorphic head has been linked to differences in feeding ecology, with broad-headed eels consuming harder prey items than narrow-headed ones. Consequently, we hypothesized that broad-heads should exhibit a cranial musculoskeletal system that increases bite force and facilitates the consumption of harder prey. Using 3D-reconstructions and a bite model, we tested this hypothesis in two life stages: the sub-adult yellow eel stage and its predecessor, the elver eel stage. This allowed us to test whether broad- and narrow-headed phenotypes show similar trait differences in both life stages and whether the dimorphism becomes more pronounced during ontogeny. We show that broad-headed eels in both stages have larger jaw muscles and a taller coronoid, which are associated with higher bite forces. This increased bite force together with the elongated upper and lower jaws in broad-headed eels can also improve grip during spinning behavior, which is used to manipulate hard prey. Head shape variation in European eel is therefore associated with musculoskeletal variation that can be linked to feeding ecology. However, although differences in muscle volume become more pronounced during ontogeny, this was not the case for skeletal features. © 2018 Anatomical Society.
Genotyping points to divergent evolution of ‘Candidatus Phytoplasma asteris’ strains causing North American grapevine yellows and strains causing aster yellows

USDA-ARS?s Scientific Manuscript database

Grapevine yellows diseases occur in cultivated grapevine (Vitis vinifera L.) on several continents, where the diseases are known by different names depending upon the identities of the causal phytoplasmas. In this study, phytoplasma strains associated with grapevine yellows disease (North American ...
Population demographics of American eels Anguilla rostrata in two Arkansas, U.S.A., catchments that drain into the Gulf of Mexico.

PubMed

Cox, C A; Quinn, J W; Lewis, L C; Adams, S R; Adams, G L

2016-03-01

The goal of this study was to compare American eel Anguilla rostrata life history in two inland river systems in Arkansas, U.S.A., that ultimately discharge into the Gulf of Mexico via the Mississippi River and the Red-Atchafalaya catchments. From 21 June 2011 to 24 April 2014, 238 yellow-phase A. rostrata were captured in the middle Ouachita River and tributaries using boat electrofishing and 39 in the lower White River using multiple sampling gears. Most of them were caught downstream of dams in both basins (61%). Medium-sized A. rostrata ranging from 225 to 350 mm total length (LT ) were the most abundant size group in the Ouachita River basin, but they were absent from the White River. Mean LT at age 4 years (i.e. youngest shared age) was 150 mm greater for the White River than the Ouachita River basin. Anguilla rostrata appeared to have a greater initial LT (i.e. minimum size upon arrival) in the White River that allowed them to reach a gonado-somatic index (IG ) of 1·5 up to 4 years earlier, and downstream migration appeared to occur 5 years earlier at 100 mm greater LT ; these differences may be related to increased river fragmentation by dams in the Ouachita River basin. Growth and maturation of A. rostrata in this study were more similar to southern populations along the Atlantic coast than other inland populations. Adult swimbladder nematodes Anguillicoloides crassus were not present in any of the 214 swimbladders inspected. Gulf of Mexico catchments may be valuable production areas for A. rostrata and data from these systems should be considered as range-wide protection and management plans are being developed. © 2016 The Fisheries Society of the British Isles.
Downstream movement of mature eels in a hydroelectric reservoir in New Zealand

USGS Publications Warehouse

Watene, E.M.; Boubee, J.A.T.; Haro, A.

2003-01-01

This study investigates the behavior of migrant eels as they approached the Patea hydroelectric dam on the West Coast of the North Island, New Zealand. Seventeen mature migrant eels (870-1,240 mm; 2,000-6,380 g) were implanted with coded acoustic transmitters and released. Their movements in the reservoir were monitored for 14 months with stationary data logging and manual tracking receivers. The downstream migration of sexually maturing eels was found to occur mainly at night, usually during, or immediately after, rainfall events. Eels tended to travel at the surface, within the upper 4 m of the water column, at speeds ranging from 16 to 89 cm/s. Upon reaching the headrace, eels typically spent time searching, presumably for an unobstructed downstream route. In order to aid downstream passage of eels at the Patea Dam, power station operators began spillway opening trials during peak migration periods. Although this allowed some migrant eels to safely pass over the dam, information on the relative effectiveness and cost of this method over other possible mitigation methods is still required. ?? Copyright by the American Fisheries Society 2003.
Leaping eels electrify threats, supporting Humboldt’s account of a battle with horses

PubMed Central

Catania, Kenneth C.

2016-01-01

In March 1800, Alexander von Humboldt observed the extraordinary spectacle of native fisherman collecting electric eels (Electrophorus electricus) by “fishing with horses” [von Humboldt A (1807) Ann Phys 25:34–43]. The strategy was to herd horses into a pool containing electric eels, provoking the eels to attack by pressing themselves against the horses while discharging. Once the eels were exhausted, they could be safely collected. This legendary tale of South American adventures helped propel Humboldt to fame and has been recounted and illustrated in many publications, but subsequent investigators have been skeptical, and no similar eel behavior has been reported in more than 200 years. Here I report a defensive eel behavior that supports Humboldt’s account. The behavior consists of an approach and leap out of the water during which the eel presses its chin against a threatening conductor while discharging high-voltage volleys. The effect is to short-circuit the electric organ through the threat, with increasing power diverted to the threat as the eel attains greater height during the leap. Measurement of voltages and current during the behavior, and assessment of the equivalent circuit, reveal the effectiveness of the behavior and the basis for its natural selection. PMID:27274074
Impact of the North Atlantic Oscillation on the trans-Atlantic migrations of the European eel (Anguilla anguilla)

NASA Astrophysics Data System (ADS)

Kettle, A. James; Bakker, Dorothee C. E.; Haines, Keith

2008-09-01

Glass eel catches and FAO (Food and Agricultural Organization) landings of the European freshwater eel (Anguilla anguilla) show a decrease over the past 20 years. The long-term trends in the time series mask an interannual fluctuation, which becomes apparent on the application of a high-pass filter and autocorrelation analysis. Both the FAO landings and the glass eel catches show interannual fluctuations with a repeat period of 6-8 years, similar to the period of the North Atlantic Oscillation (NAO). Most glass eel catch monitoring stations are in phase. The glass eel catches show a significant negative correlation with the NAO lagged by 0-2 years, consistent with the hypothesis that the positive NAO phase has an adverse impact on the larval survival in and migration from the Sargasso Sea spawning location, one year prior to the arrival of the glass eels in Europe and North Africa. The FAO landings can be divided into two groups of different phase that have an approximate correspondence to the NAO dipole in winter rainfall in Europe and North Africa. One group (P) comprises Denmark, Ireland, Morocco, Netherlands, Norway, Sweden, Tunisia, and the United Kingdom, and the other group (N) comprises France, Germany, Italy, Poland, Portugal, Spain, and Turkey. At least for the interannual fluctuations, the success of the glass eel fishery (and eel recruitment) may be coupled with the number of migrating silver eels from the N group of countries and uncoupled with the P group of countries.
Regional variation in energy storage strategies in American glass eels from Eastern Canada.

PubMed

Gaillard, Mélanie; Bernatchez, Louis; Tremblay, Réjean; Audet, Céline

2015-10-01

Energy status was analyzed in glass eels captured during two early waves of arrival at the mouths of the Mersey River, Nova Scotia, Canada (MR), and Grande-Rivière-Blanche, Québec, Canada (GRB), and according to their salinity preference (freshwater, brackish, or saltwater). Glass eels captured in the GRB estuary were larger, more pigmented, and exhibited higher whole-body glycogen, phospholipid, and sterol and wax ester contents. Those from MR had a higher condition index and a higher whole-body triacylglycerol content, suggesting different patterns of storage and/or use of energy reserves. Within a river, a delay of two weeks in estuarine arrival was characterized by significantly lower energy reserves. No differences in energy storage were observed according to salinity preference. Thus, the results revealed the occurrence of different energy storage strategies according to glass eel migration distance and duration, but not according to salinity preference. Copyright © 2015 Elsevier Inc. All rights reserved.
Inter and intra-estuary variability in ingress, condition and settlement of the American eel Anguilla rostrata: implications for estimating and understanding recruitment.

PubMed

Sullivan, M C; Wuenschel, M J; Able, K W

2009-06-01

The objective of this study was to quantify spatial and temporal variability of anguillid glass eel ingress within and between adjacent watersheds in order to help illuminate the mechanisms moderating annual recruitment. Because single fixed locations are often used to assess annual recruitment, the intra-annual dynamics of ingress across multiple sites often remains unresolved. To address this question, plankton nets and eel collectors were deployed weekly to synoptically quantify early stage Anguilla rostrata abundance at 12 sites across two New Jersey estuaries over an ingress season. Numbers of early-stage glass eels collected at the inlet mouths were moderately variable within and between estuaries over time and showed evidence for weak lunar phase and water temperature correlations. The relative condition of glass eels, although highly variable, declined significantly over the ingress season and indicated a tendency for lower condition A. rostrata to colonize sites in the lower estuary. Accumulations of glass eels and early-stage elvers retrieved from collectors (one to >1500 A. rostrata per collector) at lower estuary sites were highly variable over time, producing only weak correlations between estuaries. By way of contrast, development into late-stage elvers, coupled with the large-scale colonization of up-river sites, was highly synchronized between and within estuaries and contingent on water temperatures reaching c. 10-12 degrees C. Averaged over the ingress season, abundance estimates were remarkably consistent between paired sites across estuaries, indicating a low degree of interestuary variability. Within an estuary, however, abundance estimates varied considerably depending on location. These results and methodology have important implications for the planning and interpretation of early-stage anguillid eel surveys as well as the understanding of the dynamic nature of ingress and the spatial scales over which recruitment varies.
Gnathostoma spinigerum in live Asian swamp eels (Monopterus spp.) from food markets and wild populations, United States

USGS Publications Warehouse

Cole, Rebecca A.; Choudhury, Anindo; Nico, Leo G.; Griffin, Kathryn M.

2014-01-01

In Southeast Asia, swamp eels (Synbranchidae: Monopterus spp.) are a common source of human gnathostomiasis, a foodborne zoonosis caused by advanced third-stage larvae (AL3) of Gnathostoma spp. nematodes. Live Asian swamp eels are imported to US ethnic food markets, and wild populations exist in several states. To determine whether these eels are infected, we examined 47 eels from markets and 67 wild-caught specimens. Nematodes were identified by morphologic features and ribosomal intergenic transcribed spacer–2 gene sequencing. Thirteen (27.7%) M. cuchia eels from markets were infected with 36 live G. spinigerum AL3: 21 (58.3%) in liver; 7 (19.4%) in muscle; 5 (13.8%) in gastrointestinal tract, and 3 (8.3%) in kidneys. Three (4.5%) wild-caught M. albus eels were infected with 5 G. turgidum AL3 in muscle, and 1 G. lamothei AL3 was found in a kidney (both North American spp.). Imported live eels are a potential source of human gnathostomiasis in the United States.
Gnathostoma spinigerum in live Asian swamp eels (Monopterus spp.) from food markets and wild populations, United States.

PubMed

Cole, Rebecca A; Choudhury, Anindo; Nico, Leo G; Griffin, Kathryn M

2014-04-01

In Southeast Asia, swamp eels (Synbranchidae: Monopterus spp.) are a common source of human gnathostomiasis, a foodborne zoonosis caused by advanced third-stage larvae (AL3) of Gnathostoma spp. nematodes. Live Asian swamp eels are imported to US ethnic food markets, and wild populations exist in several states. To determine whether these eels are infected, we examined 47 eels from markets and 67 wild-caught specimens. Nematodes were identified by morphologic features and ribosomal intergenic transcribed spacer-2 gene sequencing. Thirteen (27.7%) M. cuchia eels from markets were infected with 36 live G. spinigerum AL3: 21 (58.3%) in liver; 7 (19.4%) in muscle; 5 (13.8%) in gastrointestinal tract, and 3 (8.3%) in kidneys. Three (4.5%) wild-caught M. albus eels were infected with 5 G. turgidum AL3 in muscle, and 1 G. lamothei AL3 was found in a kidney (both North American spp.). Imported live eels are a potential source of human gnathostomiasis in the United States.
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758).

PubMed

Della Torre, Camilla; Corsi, Ilaria; Arukwe, Augustine; Alcaro, Luigi; Amato, Ezio; Focardi, Silvano

2008-07-01

The aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver drug metabolizing genes and enzymes in the European eel Anguilla anguilla as a model fish species. Eels were exposed in vivo for 6h and 24h to 0.5, 1 and 2.5mg/L nominal concentrations of TNT. Expression of CYP1A, glutathione-S-transferase (pi-class; GST) and uridine-diphosphate glucuronosyltransferase (1-family) (UDPGT) genes was investigated by RT-PCR, and 7-ethoxy- and 7-methoxyresorufin-O-dealkylases (EROD, MROD), NADPH cyt c reductase (NADPH red), UDPGT and GST enzyme activities were measured by biochemical assays. An in vitro study was also performed, measuring only EROD activity. TNT exposure produced no modulation of CYP1A transcript expression while a significant inhibition of EROD enzyme activity was observed and confirmed in vitro. UDPGT transcript increased dose-dependently only at 6h while the UDPGT activity tended to increase dose-dependently at 24h. GST gene expression increased after 24h and significant increases of GST activity were observed both at 6 and 24h only at the highest TNT concentration. An increase of NADPH red activity was observed at 24h. Our results seem to indicate an inhibitory effect of TNT on CYP1A-dependent catalytic activities and a possible involvement of phase II enzymes as well as NADPH red in TNT metabolism in eels.
Electron Energy-Loss Spectroscopy (EELS)Calculation in Finite-Difference Time-Domain (FDTD) Package: EELS-FDTD

NASA Astrophysics Data System (ADS)

Large, Nicolas; Cao, Yang; Manjavacas, Alejandro; Nordlander, Peter

2015-03-01

Electron energy-loss spectroscopy (EELS) is a unique tool that is extensively used to investigate the plasmonic response of metallic nanostructures since the early works in the '50s. To be able to interpret and theoretically investigate EELS results, a myriad of different numerical techniques have been developed for EELS simulations (BEM, DDA, FEM, GDTD, Green dyadic functions). Although these techniques are able to predict and reproduce experimental results, they possess significant drawbacks and are often limited to highly symmetrical geometries, non-penetrating trajectories, small nanostructures, and free standing nanostructures. We present here a novel approach for EELS calculations using the Finite-difference time-domain (FDTD) method: EELS-FDTD. We benchmark our approach by direct comparison with results from the well-established boundary element method (BEM) and published experimental results. In particular, we compute EELS spectra for spherical nanoparticles, nanoparticle dimers, nanodisks supported by various substrates, and gold bowtie antennas on a silicon nitride substrate. Our EELS-FDTD implementation can be easily extended to more complex geometries and configurations and can be directly implemented within other numerical methods. Work funded by the Welch Foundation (C-1222, L-C-004), and the NSF (CNS-0821727, OCI-0959097).
Gnathostoma spinigerum in Live Asian Swamp Eels (Monopterus spp.) from Food Markets and Wild Populations, United States

PubMed Central

Choudhury, Anindo; Nico, Leo G.; Griffin, Kathryn M.

2014-01-01

In Southeast Asia, swamp eels (Synbranchidae: Monopterus spp.) are a common source of human gnathostomiasis, a foodborne zoonosis caused by advanced third-stage larvae (AL3) of Gnathostoma spp. nematodes. Live Asian swamp eels are imported to US ethnic food markets, and wild populations exist in several states. To determine whether these eels are infected, we examined 47 eels from markets and 67 wild-caught specimens. Nematodes were identified by morphologic features and ribosomal intergenic transcribed spacer–2 gene sequencing. Thirteen (27.7%) M. cuchia eels from markets were infected with 36 live G. spinigerum AL3: 21 (58.3%) in liver; 7 (19.4%) in muscle; 5 (13.8%) in gastrointestinal tract, and 3 (8.3%) in kidneys. Three (4.5%) wild-caught M. albus eels were infected with 5 G. turgidum AL3 in muscle, and 1 G. lamothei AL3 was found in a kidney (both North American spp.). Imported live eels are a potential source of human gnathostomiasis in the United States. PMID:24661441
Electrical Potential of Leaping Eels

PubMed Central

Catania, Kenneth C.

2017-01-01

When approached by a large, partially submerged conductor, electric eels (Electrophorus electricus) will often defend themselves by leaping from the water to directly shock the threat. Presumably, the conductor is interpreted as an approaching terrestrial or semiaquatic animal. In the course of this defensive behavior, eels first make direct contact with their lower jaw and then rapidly emerge from the water, ascending the conductor while discharging high-voltage volleys. In this study, the equivalent circuit that develops during this behavior was proposed and investigated. First, the electromotive force and internal resistance of four electric eels were determined. These values were then used to estimate the resistance of the water volume between the eel and the conductor by making direct measurements of current with the eel and water in the circuit. The resistance of the return path from the eel's lower jaw to the main body of water was then determined, based on voltage recordings, for each electric eel at the height of the defensive leap. Finally, the addition of a hypothetical target for the leaping defense was considered as part of the circuit. The results suggest the defensive behavior efficiently directs electrical current through the threat, producing an aversive and deterring experience by activating afferents in potential predators. PMID:28651251
Electrical Potential of Leaping Eels.

PubMed

Catania, Kenneth C

2017-01-01

When approached by a large, partially submerged conductor, electric eels (Electrophorus electricus) will often defend themselves by leaping from the water to directly shock the threat. Presumably, the conductor is interpreted as an approaching terrestrial or semiaquatic animal. In the course of this defensive behavior, eels first make direct contact with their lower jaw and then rapidly emerge from the water, ascending the conductor while discharging high-voltage volleys. In this study, the equivalent circuit that develops during this behavior was proposed and investigated. First, the electromotive force and internal resistance of four electric eels were determined. These values were then used to estimate the resistance of the water volume between the eel and the conductor by making direct measurements of current with the eel and water in the circuit. The resistance of the return path from the eel's lower jaw to the main body of water was then determined, based on voltage recordings, for each electric eel at the height of the defensive leap. Finally, the addition of a hypothetical target for the leaping defense was considered as part of the circuit. The results suggest the defensive behavior efficiently directs electrical current through the threat, producing an aversive and deterring experience by activating afferents in potential predators. © 2017 The Author(s) Published by S. Karger AG, Basel.
Algal-mediated ecosystem exchanges in the Eel River drainage network: towards photogrammetric mapping of color to function

NASA Astrophysics Data System (ADS)

Power, M. E.; Welter, J.; Furey, P.; Lowe, R.; Finlay, J. C.; Hondzo, M.; Limm, M.; Bode, C.; Dietrich, W. E.

2009-12-01

Seasonal algal proliferations in river networks are typically short-lived (weeks-months) but spatially extensive. They mediate important ecological and biogeochemical exchanges within and between ecosystems. We are investigating correspondence of assemblage color with ecosystem function in the nitrogen-limited Eel River of northern California. During summer base flow following winter floods, Eel algal assemblages are dominated by the green macroalga Cladophora glomerata. New growths are green, but blooms turn yellow as Cladophora filaments are colonized by epiphytic diatoms (Cocconeis spp.). Later, proliferations turn rust colored as epiphytic assemblages became dominated by Epithemia spp., diatoms that contain nitrogen-fixing cyanobacterial endosymbionts. Epithemia-encrusted Cladophora occurs at and downstream of reaches draining > 100 km2 (where summer inundated average channel widths > 25 m), coinciding with a threshold increase in concentration of total dissolved nitrogen. Areal nitrogen fixation rates are 14x higher in rusty algal proliferations than in green, and 3-4x higher than in yellow Cladophora mats. Corresponding increases in insect emergence suggest that nitrogen fixed by cyanobacterial endosymbionts is highly edible. Rates of biomass emergence from rusty Cladophora mats are 12-17 times greater than from green mats, and 8-10 times greater from rusty than from yellow Cladophora mats, because larger taxa emerge from rusty mats (Chironominae versus Ceratopogonidae in yellow mats). Photogrammetric detection of spatial coverage and color changes in algal proliferations may help us track nitrogen fluxes they mediate (riverine loading from the atmosphere via fixation, river to the watershed return via insect emergence) that link riverine to aerial, watershed, and potentially nearshore marine ecosystems at reach to basin scales.
Past distributions of the European freshwater eel from archaeological and palaeontological evidence

NASA Astrophysics Data System (ADS)

Kettle, A. J.; Heinrich, D.; Barrett, J. H.; Benecke, N.; Locker, A.

2008-07-01

The spatial distribution of the European freshwater eel ( Anguilla anguilla) was very different in historic and prehistoric times in comparison to the present. A database of the spatial and temporal distribution of eel remains in archaeological and palaeontological sites is presented and used to assess the spatial distribution of populations from the height of the last glacial maximum. The results show that the eel was absent from northern Europe until about 11 000 years ago. The reason was probably a southerly displacement of the Gulf Stream carrying the larval migration from the Sargasso Sea. However, additional factors preventing eel populations in northern Europe may have also been the colder temperatures in the Arctic tundra landscape that existed at the time and the extreme distance to the European Atlantic coast along the Channel River. The archaeological record shows that eels were absent from the Baltic Sea until about 6700 cal BC, but there is some indication of an earlier presence during the Yoldia Sea stage at the beginning of the Holocene. Only in southern Europe south of the Gironde river basin were eel populations maintained through the last glaciation. The species may have survived the last glaciation in a relatively restricted area in the Mediterranean and the Atlantic coast of western Europe. Published palaeontological and genetic information gives important insights into climatic, geologic, and tectonic events on longer time scales. The oldest subfossil remains from Pleistocene sediments in northern Europe are approaching the age of the estimated genetic divergence of the European and American eel populations, and hence the species identity of the oldest subfossil remains may be ambiguous.

Impacts of Interannual Ocean Circulation Variability on Japanese Eel Larval Migration in the Western North Pacific Ocean.

PubMed

Chang, Yu-Lin; Sheng, Jinyu; Ohashi, Kyoko; Béguer-Pon, Mélanie; Miyazawa, Yasumasa

2015-01-01

The Japanese eel larvae hatch near the West Mariana Ridge seamount chain and travel through the North Equatorial Current (NEC), the Kuroshio, and the Subtropical Countercurrent (STCC) region during their shoreward migration toward East Asia. The interannual variability of circulation over the subtropical and tropical regions of the western North Pacific Ocean is affected by the Philippines-Taiwan Oscillation (PTO). This study examines the effect of the PTO on the Japanese eel larval migration routes using a three-dimensional (3D) particle tracking method, including vertical and horizontal swimming behavior. The 3D circulation and hydrography used for particle tracking are from the ocean circulation reanalysis produced by the Japan Coastal Ocean Predictability Experiment 2 (JCOPE2). Our results demonstrate that bifurcation of the NEC and the strength and spatial variation of the Kuroshio affect the distribution and migration of eel larvae. During the positive phase of PTO, more virtual eels ("v-eels") can enter the Kuroshio to reach the south coast of Japan and more v-eels reach the South China Sea through the Luzon Strait; the stronger and more offshore swing of the Kuroshio in the East China Sea leads to fewer eels entering the East China Sea and the onshore movement of the Kuroshio to the south of Japan brings the eels closer to the Japanese coast. Significant differences in eel migration routes and distributions regulated by ocean circulation in different PTO phases can also affect the otolith increment. The estimated otolith increment suggests that eel age tends to be underestimated after six months of simulation due to the cooler lower layer temperature. Underestimation is more significant in the positive PTO years due to the wide distribution in higher latitudes than in the negative PTO years.
Differential gene expression analysis in European eels (Anguilla anguilla, L. 1758) naturally infected by macroparasites.

PubMed

Fazio, G; Moné, H; Lecomte-Finiger, R; Sasal, P

2008-06-01

We analyzed the relationships between the macroparasite community of the European eel and the expression of genes involved in the host physiology during its continental life. The genes studied are implicated in (1) host response to environmental stress, i.e., heat shock protein 70 (HSP70) and metallothionein (MT); (2) osmoregulation, i.e., beta thyroid hormone receptor (betaTHR) and Na+/K+ATPase; and (3) silvering, i.e., betaTHR, freshwater rod opsin (FWO), and deep-sea rod opsin (DSO). All were enumerated by quantitative reverse-transcription polymerase chain reaction. The epizootiological results for 93 yellow eels caught in the Salses-Leucate Lagoon (France) included 11 species: 1 nematode, 2 acanthocephalans, 1 monogenean, and 7 digeneans. The molecular results revealed (1) a significant negative relationship between digenean abundance and the expression level of all the tested genes, except FWO; (2) a significant negative relationship between the abundance of the nematode Anguillicola crassus and the expression level of the Na+/K+ATPase gene; and (3) a significant positive relationship between the A. crassus abundance and the expression level of the MT gene. Eels infected with digeneans had, on average, a lower level of expressed genes. We hypothesize that the parasites may disturb the eel's ability to withstand environmental stress and delay their migration to the Sargasso Sea because of degeneration of the gut. We further propose that the effect of the invasive species, A. crassus, on the gene expression was mainly linked to an increased trophic activity of infected eels. Moreover, it is possible that the parasite may have an effect on the fish's migratory behavior, which is tied to reproductive purposes. Additional work, including an experimental approach, is required to confirm our hypotheses.
Assessment of persistent brominated and chlorinated organic contaminants in the European eel (Anguilla anguilla) in Flanders, Belgium: Levels, profiles and health risk.

PubMed

Malarvannan, Govindan; Belpaire, Claude; Geeraerts, Caroline; Eulaers, Igor; Neels, Hugo; Covaci, Adrian

2014-06-01

Pooled yellow European eel (Anguilla anguilla (L.)) samples, consisting of 3-10 eels, collected between 2000 and 2009 from 60 locations in Flanders (Belgium) were investigated for persistent contaminants, such as polybrominated diphenyl ethers (PBDEs), hexabromocyclododecanes (HBCDs), polychlorinated biphenyls (PCBs) and dichlorodiphenyltrichloroethane and its metabolites (DDTs). The current study expands the knowledge regarding these contaminant concentrations, their patterns and distribution profiles in aquatic ecosystems. PBDEs, HBCDs, PCBs, and DDTs were detected in all eel samples and some samples had high concentrations (up to 1400, 9500, 41,600 and 7000ng/g lw, respectively). PCB levels accounted for the majority of the contamination in most samples. The high variability in PBDE, HBCD, PCB and DDT concentrations reported here is likely due to the variety in sampling locations demonstrating variable local pollution pressures, from highly industrialised areas to small rural creeks. Among PBDEs, BDE-47 (57% contribution to the sum PBDEs), -100 (19%) and -99 (15%) were the predominant congeners, similar to the composition reported in the literature in eel samples. For HBCDs, α-HBCD (74%) was predominant followed by γ-(22%) and β-HBCD (4%) isomers in almost all eel samples. CB-153 (19%) was the most dominant PCB congener, closely followed by CB-138 (11%), CB-180 (9%), CB-187 (8%) and CB-149 (7%). The contribution to the total human exposure through local wild eel consumption was also highly variable. Intake of PBDEs and HBCDs, through consumption of wild eel, was below the RfD values for the average population (consuming on average 2.9g eel/day). At 16 out 60 sites, eels exceeded largely the new EU consumption threshold for PCBs (300ng/g ww for the sum of 6 indicator PCBs). The current data shows an on-going exposure of Flemish eels to PBDEs, HBCDs, PCBs and DDTs through indirect release from contaminated sediments or direct releases from various industries
Accumulation of persistent organic pollutants in consumers of eel from polluted rivers compared to marketable eel.

PubMed

van den Dungen, Myrthe W; Kok, Dieuwertje E; Polder, Anuschka; Hoogenboom, Ron L A P; van Leeuwen, Stefan P J; Steegenga, Wilma T; Kampman, Ellen; Murk, Albertinka J

2016-12-01

Globally, many river sediments are seriously contaminated with persistent organic pollutants (POPs) known to accumulate in aquatic food. In the Netherlands, toxicological risks of human exposure to dioxins and dioxin-like compounds led to a ban on eel fishing in the Rhine-Meuse delta. The aim of this study is to investigate differences in serum POP levels in consumers of eel from high-polluted areas and consumers of eel from low-polluted areas or aquaculture. In total 80 Dutch men were included, aged 40-70 years, with a habitual eel consumption of at least one portion (150 g) per month. Total levels of dioxins and dioxin-like compounds were measured in serum of all participants with the DR CALUX bioassay, validated with GC-MS. For a subgroup of 38 participants extensive POP measurements were performed. We revealed that consumption of eel from polluted rivers resulted in 2.5 and up to 10 times increased levels of dioxins and polychlorinated biphenyls (PCBs) respectively compared to controls. The highest PCB levels were detected for PCB 153, with a median level of 896 ng/g lipid and a maximum level of 5000 ng/g lipid in the high-exposed group. Furthermore, hydroxylated PCB metabolites (OH-PCBs: sum of 4-OH-CB107, 4-OH-CB146, 4'-OH-CB172, and 4-OH-CB187) were 8 times higher in men who consumed eel from polluted areas, and detected at levels (median 4.5 ng/g ww) reported to cause adverse health effects. Also, the majority of the perfluoroalkyl substances (PFASs) were significantly higher in consumers of eel from pullulated areas. In conclusion, this study is the first to reveal that (past) consumption of eel from polluted rivers resulted in high body burdens of dioxins, PCBs, OH-PCBs and PFASs. We confirmed the predictions made in a former risk assessment, and the high levels of dioxins and dioxin-like compounds as well as the OH-PCBs are of health concern. Copyright © 2016 Elsevier Ltd. All rights reserved.
Implications of facultative catadromy in Anguilla anguilla. Does individual migratory behaviour influence eel spawner quality?

NASA Astrophysics Data System (ADS)

Marohn, Lasse; Jakob, Eva; Hanel, Reinhold

2013-03-01

European eel (Anguilla anguilla) recruitment is declining dramatically since the 1980s. Causes for this decline are explained by a combination of environmental changes basically affecting oceanic larval stages and a variety of anthropogenic impacts during the continental phase. Today, evidence is growing that poor silver eel quality has a major impact on reproductive success, implying that habitat quality plays a key role in stock decline. Since eels are frequently moving through a variety of different habitats during their life cycle, a better understanding of the implications of individual diadromous behaviour and habitat choice on spawner quality are crucial for management considerations for a stock recovery. The present study tested whether individual migratory behaviour and habitat choice of European eels affect spawner quality. Therefore, the migratory behaviour of 287 European eels from marine, brackish and freshwater stations in the North Sea, the Baltic Sea and from Northern German inland waters was examined by otolith strontium/calcium analysis. All individuals were classified either as freshwater residents, coastal residents, downstream shifters, upstream shifters or interhabitat shifters. As indicators for eel quality, muscle fat content, infection with the introduced swimbladder nematode Anguillicoloides crassus and body length at onset of spawning migration were assessed. Results indicate that individuals that exclusively inhabited freshwaters had significantly lower muscle fat contents and were more seriously infected with A. crassus than eels that never entered freshwaters. Since high fat contents are considered as prerequisites for a successful transoceanic spawning migration and high A. crassus loads have a negative impact on condition, this study outlines the importance of brackish waters as eel habitats in temperate latitudes. Furthermore, it questions the net benefit of stocking programs for the European eel population, since they include the
Magnetic compass orientation in the European eel.

PubMed

Durif, Caroline M F; Browman, Howard I; Phillips, John B; Skiftesvik, Anne Berit; Vøllestad, L Asbjørn; Stockhausen, Hans H

2013-01-01

European eel migrate from freshwater or coastal habitats throughout Europe to their spawning grounds in the Sargasso Sea. However, their route (~ 6000 km) and orientation mechanisms are unknown. Several attempts have been made to prove the existence of magnetoreception in Anguilla sp., but none of these studies have demonstrated magnetic compass orientation in earth-strength magnetic field intensities. We tested eels in four altered magnetic field conditions where magnetic North was set at geographic North, South, East, or West. Eels oriented in a manner that was related to the tank in which they were housed before the test. At lower temperature (under 12°C), their orientation relative to magnetic North corresponded to the direction of their displacement from the holding tank. At higher temperatures (12-17°C), eels showed bimodal orientation along an axis perpendicular to the axis of their displacement. These temperature-related shifts in orientation may be linked to the changes in behavior that occur between the warm season (during which eels are foraging) and the colder fall and winter (during which eels undertake their migrations). These observations support the conclusion that 1. eels have a magnetic compass, and 2. they use this sense to orient in a direction that they have registered moments before they are displaced. The adaptive advantage of having a magnetic compass and learning the direction in which they have been displaced becomes clear when set in the context of the eel's seaward migration. For example, if their migration is halted or blocked, as it is the case when environmental conditions become unfavorable or when they encounter a barrier, eels would be able to resume their movements along their old bearing when conditions become favorable again or when they pass by the barrier.
Metabolizable energy in Chinese tallow fruit for Yellow-rumped Warblers, Northern Cardinals, and American Robins

USGS Publications Warehouse

Baldwin, M.J.; Barrow, W.C.; Jeske, C.; Rohwer, F.C.

2008-01-01

The invasive exotic Chinese tallow tree (Triadica sebifera) produces an abundant fruit crop, which is primarily bird-dispersed. The fruit pulp of tallow is lipid-rich, high in saturated fatty acids, and consumed by many bird species. Long-chained fatty acids can be difficult for many birds to digest and we investigated the ability of tallow consumers to assimilate energy in the pulp. We used the total collection method and compared apparent metabolizable energy (AME) of tallow fruit for three species of birds with differing fruit composition in their natural diets. All birds exhibited nitrogen deficits and lost body mass during the trials. Northern Cardinals (Cardinalis cardinalis) lost more mass (8.73%/day) than Yellow-rumped Warblers (Dendroica coronata) (5.29%/day) and American Robins (Turdus migratorius) (5.48%/day), and had larger nitrogen deficits (-120.1 mg N/g diet) than both species as well (-36.4 mg N/g diet and -68.9 mg N/g diet, respectively). Food intake relative to metabolic body mass was highest in Yellow-rumped Warblers (0.70 g-dry/g 0.75??day). Northern Cardinal and American Robin food intake was lower and did not differ from each other (both species: 0.13 g-dry/g 0.75??day). Nitrogen corrected values of AME were used to make species comparisons. Yellow-rumped-Warblers exhibited the highest values of AME (30.00 kJ/g), followed by American Robins (23.90 kJ/g), and Northern Cardinals (14.34 kJ/g). We suggest tallow may be an important winter food source for Yellow-rumped Warblers where their ranges overlap.
Enhancement and management of eel fisheries affected by hydroelectric dams in New Zealand

USGS Publications Warehouse

Boubee, J.; Chisnall, B.; Watene, E.; Williams, E.; Roper, D.; Haro, A.

2003-01-01

Two freshwater anguillid eel species, Anguilla australis and A. dieffenbachia, form the basis of important traditional, recreational, and commercial fisheries in New Zealand. These fisheries have been affected by the damming of many of the major waterways for hydroelectric generation. To create fisheries in reservoirs that would be otherwise inaccessible, elvers have been transferred from the base of dams into habitats upstream. Operations in three catchments: the Patea River (Lake Rotorangi), Waikato River (eight reservoirs notably the two lowermost, lakes Karapiro and Arapuni), and Rangitaiki River (lakes Matahina and Aniwhenua) are discussed. In all reservoirs, the transfers have successfully established fishable populations within six years of the first transfers and, in Lake Arapuni eels have reached the marketable size of 220 g in less than four years. In comparison, it typically takes from 13 to 17 years before eel populations are fishable in the lower Waikato River where direct access to the sea is available. Telemetry and monitoring at the screens and tailraces of several power stations have been used to determine migration timing, triggers, and pathways of mature eels. Successful downstream transfer of mature migrating adults has been achieved by spillway opening and netting in headraces during rain events in autumn, but means of preventing eels from impinging and entraining at the intakes are still required. An integrated, catchment-wide management system will be required to ensure sustainability of the fisheries. ?? Copyright by the American Fisheries Society 2003.
Adaptive Diversification Between Yellow Fever Virus West African and South American Lineages: A Genome-Wide Study.

PubMed

Li, Yan; Yang, Zexiao

2017-03-01

AbstractYellow fever virus (YFV) has emerged as the causative agent of a vector-borne disease with devastating mortality in the tropics of Africa and the Americas. YFV phylogenies indicate that the isolates collected from West Africa, East and Central Africa, and South America cluster into different lineages and the virus spread into the Americas from Africa. To determine the nature of genetic variation accompanying the intercontinental epidemic, we performed a genome-wide evolutionary study on the West African and South American lineages of YFV. Our results reveal that adaptive genetic diversification has occurred on viral nonstructural protein 5 (NS5), which is crucially required for viral genome replication, in the early epidemic phase of these currently circulating lineages. Furthermore, major amino acid changes relevant to the adaptive diversification generally cluster in different structural regions of NS5 in a lineage-specific manner. These results suggest that YFV has experienced adaptive diversification in the epidemic spread between the continents and shed insights into the genetic determinants of such diversification, which might be beneficial for understanding the emergence and re-emergence of yellow fever as an important global public health issue.
Magnetic Compass Orientation in the European Eel

PubMed Central

Durif, Caroline M. F.; Browman, Howard I.; Phillips, John B.; Skiftesvik, Anne Berit; Vøllestad, L. Asbjørn; Stockhausen, Hans H.

2013-01-01

European eel migrate from freshwater or coastal habitats throughout Europe to their spawning grounds in the Sargasso Sea. However, their route (∼ 6000 km) and orientation mechanisms are unknown. Several attempts have been made to prove the existence of magnetoreception in Anguilla sp., but none of these studies have demonstrated magnetic compass orientation in earth-strength magnetic field intensities. We tested eels in four altered magnetic field conditions where magnetic North was set at geographic North, South, East, or West. Eels oriented in a manner that was related to the tank in which they were housed before the test. At lower temperature (under 12°C), their orientation relative to magnetic North corresponded to the direction of their displacement from the holding tank. At higher temperatures (12–17°C), eels showed bimodal orientation along an axis perpendicular to the axis of their displacement. These temperature-related shifts in orientation may be linked to the changes in behavior that occur between the warm season (during which eels are foraging) and the colder fall and winter (during which eels undertake their migrations). These observations support the conclusion that 1. eels have a magnetic compass, and 2. they use this sense to orient in a direction that they have registered moments before they are displaced. The adaptive advantage of having a magnetic compass and learning the direction in which they have been displaced becomes clear when set in the context of the eel’s seaward migration. For example, if their migration is halted or blocked, as it is the case when environmental conditions become unfavorable or when they encounter a barrier, eels would be able to resume their movements along their old bearing when conditions become favorable again or when they pass by the barrier. PMID:23554997
Oceanic migration and spawning of anguillid eels.

PubMed

Tsukamoto, K

2009-06-01

Many aspects of the life histories of anguillid eels have been revealed in recent decades, but the spawning migrations of their silver eels in the open ocean still remains poorly understood. This paper overviews what is known about the migration and spawning of anguillid species in the ocean. The factors that determine exactly when anguillid eels will begin their migrations are not known, although environmental influences such as lunar cycle, rainfall and river discharge seem to affect their patterns of movement as they migrate towards the ocean. Once in the ocean on their way to the spawning area, silver eels probably migrate in the upper few hundred metres, while reproductive maturation continues. Although involvement of a magnetic sense or olfactory cues seems probable, how they navigate or what routes they take are still a matter of speculation. There are few landmarks in the open ocean to define their spawning areas, other than oceanographic or geological features such as oceanic fronts or seamounts in some cases. Spawning of silver eels in the ocean has never been observed, but artificially matured eels of several species have exhibited similar spawning behaviours in the laboratory. Recent collections of mature adults and newly spawned preleptocephali in the spawning area of the Japanese eel Anguilla japonica have shown that spawning occurs during new moon periods in the North Equatorial Current region near the West Mariana Ridge. These data, however, show that the latitude of the spawning events can change among months and years depending on oceanographic conditions. Changes in spawning location of this and other anguillid species may affect their larval transport and survival, and appear to have the potential to influence recruitment success. A greater understanding of the spawning migration and the choice of spawning locations by silver eels is needed to help conserve declining anguillid species.
Simulating the Oceanic Migration of Silver Japanese Eels

PubMed Central

Chang, Yu-Lin; Miyazawa, Yasumasa; Béguer-Pon, Mélanie

2016-01-01

The oceanic migration of silver Japanese eels starts from their continental growth habitats in East Asia and ends at the spawning area near the West Mariana Ridge seamount chain. However, the actual migration routes remain unknown. In this study, we examined the possible oceanic migration routes and strategies of silver Japanese eels using a particle tracking method in which virtual eels (v-eels) were programmed to move vertically and horizontally in an ocean circulation model (Japan Coastal Ocean Predictability Experiment 2, JCOPE2). Four horizontal swimming strategies were tested: random heading, true navigation (readjusted heading), orientation toward the spawning area (fixed heading), and swimming against the Kuroshio. We found that all strategies, except random swimming, allowed v-eels swimming at 0.65 m s−1 to reach the spawning area within eight months after their departure from the south coast of Japan (end of the spawning season). The estimated minimum swimming speed required to reach the area spawning within eight months was 0.1 m s−1 for true navigation, 0.12 m s−1 for constant compass heading, and 0.35 m s−1 for swimming against the Kuroshio. The lowest swimming speed estimated from tracked Japanese eels at sea was 0.03 m.s−1, which would not allow them to reach the spawning area within eight months, through any of the tested orientation strategies. Our numerical experiments also showed that ocean circulation significantly affected the migration of Japanese v-eels. A strong Kuroshio could advect v-eels further eastward. In addition, western Pacific ocean currents accelerated the migration of navigating v-eels. The migration duration was shortened in years with a stronger southward flow, contributed by a stronger recirculation south of Japan, an enhanced subtropical gyre, or a higher southward Kuroshio velocity. PMID:26982484
Simulating the Oceanic Migration of Silver Japanese Eels.

PubMed

Chang, Yu-Lin; Miyazawa, Yasumasa; Béguer-Pon, Mélanie

2016-01-01

The oceanic migration of silver Japanese eels starts from their continental growth habitats in East Asia and ends at the spawning area near the West Mariana Ridge seamount chain. However, the actual migration routes remain unknown. In this study, we examined the possible oceanic migration routes and strategies of silver Japanese eels using a particle tracking method in which virtual eels (v-eels) were programmed to move vertically and horizontally in an ocean circulation model (Japan Coastal Ocean Predictability Experiment 2, JCOPE2). Four horizontal swimming strategies were tested: random heading, true navigation (readjusted heading), orientation toward the spawning area (fixed heading), and swimming against the Kuroshio. We found that all strategies, except random swimming, allowed v-eels swimming at 0.65 m s-1 to reach the spawning area within eight months after their departure from the south coast of Japan (end of the spawning season). The estimated minimum swimming speed required to reach the area spawning within eight months was 0.1 m s-1 for true navigation, 0.12 m s-1 for constant compass heading, and 0.35 m s-1 for swimming against the Kuroshio. The lowest swimming speed estimated from tracked Japanese eels at sea was 0.03 m.s-1, which would not allow them to reach the spawning area within eight months, through any of the tested orientation strategies. Our numerical experiments also showed that ocean circulation significantly affected the migration of Japanese v-eels. A strong Kuroshio could advect v-eels further eastward. In addition, western Pacific ocean currents accelerated the migration of navigating v-eels. The migration duration was shortened in years with a stronger southward flow, contributed by a stronger recirculation south of Japan, an enhanced subtropical gyre, or a higher southward Kuroshio velocity.
Toxicity of 5% rotenone to nonindigenous Asian swamp eels

USGS Publications Warehouse

Schofield, P.J.; Nico, L.G.

2007-01-01

Our primary goal was to determine whether rotenone would be a useful control against introduced populations of Asian swamp eels (family Synbranchidae, genus Monopterus). We report the results of a laboratory experiment comparing the efficacy of various rotenone concentrations (1, 2, 4, and 8 mg of 5% liquid rotenone/L of water) in killing nonindigenous swamp eels of various sizes (1-350 g) from the three known Florida populations. Although most small swamp eels were killed at concentrations of 2 and 4 mg/L. 100% mortality of adult swamp eels was achieved only at 8 mg/L. We conclude that the effective use of rotenone to control established Florida swamp eel populations would be difficult, based on the relatively high concentration of rotenone needed to kill swamp eels; the complexity of the swamp eel's habitat; and our observations of the species' habitat use and behavior, including its widespread distribution and life history characteristics (e.g., burrowing and overland movement) that enhance its invasion and survival in multiple environments. Nevertheless, control of swamp eels may be achieved in certain situations. A combination of rotenone and electroshocking may be an effective way to eradicate swamp eels from small water bodies and to control populations in larger habitats. However, we are cautious in this recommendation and provide details related to the technical aspects of this type of strategy and caveats related to the toxicity of the chemical.
Enteric neuroplasticity in seawater-adapted European eel (Anguilla anguilla).

PubMed

Sorteni, C; Clavenzani, P; De Giorgio, R; Portnoy, O; Sirri, R; Mordenti, O; Di Biase, A; Parmeggiani, A; Menconi, V; Chiocchetti, R

2014-02-01

European eels live most of their lives in freshwater until spawning migration to the Sargasso Sea. During seawater adaptation, eels modify their physiology, and their digestive system adapts to the new environment, drinking salt water to compensate for the continuous water loss. In that period, eels stop feeding until spawning. Thus, the eel represents a unique model to understand the adaptive changes of the enteric nervous system (ENS) to modified salinity and starvation. To this purpose, we assessed and compared the enteric neuronal density in the cranial portion of the intestine of freshwater eels (control), lagoon eels captured in brackish water before their migration to the Sargasso Sea (T0), and starved seawater eels hormonally induced to sexual maturity (T18; 18 weeks of starvation and treatment with standardized carp pituitary extract). Furthermore, we analyzed the modification of intestinal neuronal density of hormonally untreated eels during prolonged starvation (10 weeks) in seawater and freshwater. The density of myenteric (MP) and submucosal plexus (SMP) HuC/D-immunoreactive (Hu-IR) neurons was assessed in wholemount preparations and cryosections. The number of MP and SMP HuC/D-IR neurons progressively increased from the freshwater to the salty water habitat (control > T0 > T18; P < 0.05). Compared with freshwater eels, the number of MP and SMP HuC/D-IR neurons significantly increased (P < 0.05) in the intestine of starved untreated salt water eels. In conclusion, high salinity evokes enteric neuroplasticity as indicated by the increasing number of HuC/D-IR MP and SMP neurons, a mechanism likely contributing to maintaining the body homeostasis of this fish in extreme conditions. © 2013 Anatomical Society.
Enteric neuroplasticity in seawater-adapted European eel (Anguilla anguilla)

PubMed Central

Sorteni, C; Clavenzani, P; De Giorgio, R; Portnoy, O; Sirri, R; Mordenti, O; Di Biase, A; Parmeggiani, A; Menconi, V; Chiocchetti, R

2014-01-01

European eels live most of their lives in freshwater until spawning migration to the Sargasso Sea. During seawater adaptation, eels modify their physiology, and their digestive system adapts to the new environment, drinking salt water to compensate for the continuous water loss. In that period, eels stop feeding until spawning. Thus, the eel represents a unique model to understand the adaptive changes of the enteric nervous system (ENS) to modified salinity and starvation. To this purpose, we assessed and compared the enteric neuronal density in the cranial portion of the intestine of freshwater eels (control), lagoon eels captured in brackish water before their migration to the Sargasso Sea (T0), and starved seawater eels hormonally induced to sexual maturity (T18; 18 weeks of starvation and treatment with standardized carp pituitary extract). Furthermore, we analyzed the modification of intestinal neuronal density of hormonally untreated eels during prolonged starvation (10 weeks) in seawater and freshwater. The density of myenteric (MP) and submucosal plexus (SMP) HuC/D-immunoreactive (Hu-IR) neurons was assessed in wholemount preparations and cryosections. The number of MP and SMP HuC/D-IR neurons progressively increased from the freshwater to the salty water habitat (control > T0 > T18; P < 0.05). Compared with freshwater eels, the number of MP and SMP HuC/D-IR neurons significantly increased (P < 0.05) in the intestine of starved untreated salt water eels. In conclusion, high salinity evokes enteric neuroplasticity as indicated by the increasing number of HuC/D-IR MP and SMP neurons, a mechanism likely contributing to maintaining the body homeostasis of this fish in extreme conditions. PMID:24433383
Stage-specific distribution models can predict eel (Anguilla anguilla) occurrence during settlement in coastal lagoons

NASA Astrophysics Data System (ADS)

Leone, C.; Zucchetta, M.; Capoccioni, F.; Gravina, M. F.; Franzoi, P.; Ciccotti, E.

2016-03-01

Eel (Anguilla anguilla) is a catadromous fish species typical of Mediterranean coastal lagoons, that currently suffers from several anthropogenic and natural impacts. These are thought to be the cause of a stock-wide decline that this panmictic species is facing, in inland and coastal waters of Europe and North Africa. The decline affects both adult phases and recruitment, i.e. glass eel arrival to coastal waters and their ascent to inland waters. Quantitative features of eel recruitment reflect a transoceanic global scale, but also depend on local environmental conditions, the latter also affecting settlement dynamics in transitional waters. There is only little information on the dynamics of these two processes in coastal lagoons, notwithstanding the paramount importance of both in sustaining local stocks abundance and their demographic structure for this typical but also economically important inhabitant of Mediterranean lagoons, habitats that constitute an important share of the eel distribution area. The present study aims, therefore, to clarify space and time dynamics of local scale recruitment and of settlement in a coastal lagoon in the Mediterranean area, also by setting up a specific methodological approach. For this purpose, data from field surveys in combination with Species Distribution Models (SDMs) have been used in order to relate distribution of eel juvenile stages to the environmental conditions within the lagoon. Specifically, models were calibrated to quantify the relationship between presence of juvenile eel and the main environmental drivers, with the aim of identifying potential habitats for eel settlement within the lagoon. Results gained by modelling suggest certain spatial and temporal colonization patterns for the juvenile eel in the Fogliano lagoon, a typical Mediterranean coastal lake. The modelling approach has therefore proved to be a useful tool for predicting habitats for eel recruitment at the local scale and settlement, because
Expression and localization of aquaporin 1b during oocyte development in the Japanese eel (Anguilla japonica)

PubMed Central

2011-01-01

To elucidate the molecular mechanisms underling hydration during oocyte maturation, we characterized the structure of Japanese eel (Anguilla japonica) novel-water selective aquaporin 1 (AQP1b) that thought to be involved in oocyte hydration. The aqp1b cDNA encodes a 263 amino acid protein that includes the six potential transmembrane domains and two Asn-Pro-Ala motifs. Reverse transcription-polymerase chain reaction showed transcription of Japanese eel aqp1b in ovary and testis but not in the other tissues. In situ hybridization studies with the eel aqp1b cRNA probe revealed intense eel aqp1b signal in the oocytes at the perinucleolus stage and the signals became faint during the process of oocyte development. Light microscopic immunocytochemical analysis of ovary revealed that the Japanese eel AQP1b was expressed in the cytoplasm around the yolk globules which were located in the peripheral region of oocytes during the primary yolk globule stage; thereafter, the immunoreactivity was observed throughout the cytoplasm of oocyte as vitellogenesis progressed. The immunoreactivity became localized around the large membrane-limited yolk masses which were formed by the fusion of yolk globules during the oocyte maturation phase. These results together indicate that AQP1b, which is synthesized in the oocyte during the process of oocyte growth, is essential for mediating water uptake into eel oocytes. PMID:21615964
33 CFR 117.598 - Eel Pond Channel.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Eel Pond Channel. 117.598 Section 117.598 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.598 Eel Pond Channel. The...
33 CFR 117.598 - Eel Pond Channel.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Eel Pond Channel. 117.598 Section 117.598 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.598 Eel Pond Channel. The...

33 CFR 117.598 - Eel Pond Channel.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Eel Pond Channel. 117.598 Section 117.598 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.598 Eel Pond Channel. The...
33 CFR 117.598 - Eel Pond Channel.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Eel Pond Channel. 117.598 Section 117.598 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.598 Eel Pond Channel. The...
33 CFR 117.598 - Eel Pond Channel.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Eel Pond Channel. 117.598 Section 117.598 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.598 Eel Pond Channel. The...
Is feeding behaviour related to glass eel propensity to migrate?

NASA Astrophysics Data System (ADS)

Bureau du Colombier, Sarah; Lambert, Patrick; Bardonnet, Agnès

2008-11-01

Several studies have shown that eel diadromy is facultative and that migratory divergences may appear during glass eel estuarine migration. The origin of the differences in migratory behaviour among glass eels remains unclear but initial evidence supports the role of individual energetic and thyroidal status. Even if starvation is usually associated with glass eel migration, feeding does seem to occur in some glass eels. The aim of the present study was to investigate feeding behaviour and glass eel growth in relation to the propensity to migrate. Feeding rate and weight gain were higher in fish having a high propensity to migrate (M + fish) than in fish having a low propensity to migrate (M - fish) in fed glass eels, whereas no clear difference in the variation in body weight was observed among unfed fish (controls). M - fish initially had lower percent dry weight than M + fish, which suggests a link between appetite, propensity to migrate, and energy content. We discuss the role played by endocrine signals on these processes. In fish, thyroid hormones contribute to the control of growth and development. In addition, they play a role in flatfish and leptocephalus metamorphosis and appear to be involved in smolt and glass eel migratory behaviour. As such, they represent a good candidate which would promote the propensity to migrate as well as digestive system development. Their role in the hormonal control of food intake however remains vague. The large and sharp decline in glass eel abundances observed since the 1980s could partly be explained by changes in ocean productivity. If so, it could be accompanied by a decrease in glass eel energy stores. The ability to resume feeding in the course of the estuarine crossing would then represent a serious advantage to maintain energy levels compatible with migration.
Fish measurement using Android smart phone: the example of swamp eel

NASA Astrophysics Data System (ADS)

Chen, Baisong; Fu, Zhuo; Ouyang, Haiying; Sun, Yingze; Ge, Changshui; Hu, Jing

The body length and weight are critical physiological parameters for fishes, especially eel-like fishes like swamp eel(Monopterusalbus).Fast and accurate measuring of body length is significant for swamp eel culturing as well as its resource investigation and protection. This paper presents an Android smart phone-based photogrammetry technology for measuring and estimating the length and weight of swamp eel. This method utilizes the feature that the ratio of lengths of two objects within an image is equal to that of in reality to measure the length of swamp eels. And then, it estimates the weight via a pre-built length-weight regression model. Analysis and experimental results have indicated that this method is a fast and accurate method for length and weight measurements of swamp eel. The cross-validation results shows that the RMSE (root-mean-square error) of total length measurement of swamp eel is0.4 cm, and the RMSE of weight estimation is 11 grams.
Are dioxin-like contaminants responsible for the eel ( Anguilla anguilla) drama?

NASA Astrophysics Data System (ADS)

Palstra, A. P.; van Ginneken, V. J. T.; Murk, A. J.; van den Thillart, G. E. E. J. M.

2006-03-01

Eel populations worldwide are dangerously close to collapsing. Our study is the first to show that current levels of dioxin-like contaminants are strong candidates because of their devastating effects on development and survival of eel embryos. Female and male silver eels were artificially stimulated to maturation and reproduction by treatment with carp pituitary extracts and hCG, respectively. During maturation of female European silver eels, about 60 g fat per kg eel is incorporated in the oocytes. Together with the fat, however, persistent organic pollutants such as dioxin-like polychlorinated biphenyls (PCBs) are incorporated too. The total dioxin-like toxic potency of the individual gonad batches was determined as 2,3,7,8-tetrachlorodibenzo- p-dioxine equivalents (TEQs), using an in vitro reporter gene assay. The observed differences in development and survival showed a significant negative correlation with the TEQ levels in the gonads, already at levels far below the maximal allowable level for fish consumption, i.e., 4 ng TEQ/kg fish. The clear inverse relationship between the TEQ level and the survival period of the fertilised eggs strongly suggests that the current levels of dioxin-like compounds seriously impair the reproduction of the European eel. The peak of the environmental levels of dioxin-like PCBs and the decline of eel coincide worldwide, further suggesting that, in addition to other threats, these contaminants contributed significantly to the current collapse in eel populations.
Phases and structures of sunset yellow and disodium cromoglycate mixtures in water.

PubMed

Yamaguchi, Akihiro; Smith, Gregory P; Yi, Youngwoo; Xu, Charles; Biffi, Silvia; Serra, Francesca; Bellini, Tommaso; Zhu, Chenhui; Clark, Noel A

2016-01-01

We study phases and structures of mixtures of two representative chromonic liquid crystal materials, sunset yellow FCF (SSY) and disodium cromoglycate (DSCG), in water. A variety of combinations of isotropic, nematic (N), and columnar (also called M) phases are observed depending on their concentrations, and a phase diagram is made. We find a tendency for DSCG-rich regions to show higher-order phases while SSY-rich regions show lower-order ones. We observe uniform mesophases only when one of the materials is sparse in the N phases. Their miscibility in M phases is so low that essentially complete phase separation occurs. X-ray scattering and spectroscopy studies confirm that SSY and DSCG molecules do not mix when they form chromonic aggregates and neither do their aggregates when they form M phases.
Morphological alterations in the liver of yellow perch (Perca flavescens) from a biological mercury hotspot.

PubMed

Müller, Anne-Katrin; Brinkmann, Markus; Baumann, Lisa; Stoffel, Michael H; Segner, Helmut; Kidd, Karen A; Hollert, Henner

2015-11-01

Mercury (Hg) contamination is a global issue due to its anthropogenic release, long-range transport, and deposition in remote areas. In Kejimkujik National Park and National Historic Site, Nova Scotia, Canada, high concentrations of total mercury (THg) were found in tissues of yellow perch (Perca flavescens). The aim of this study was to evaluate a possible relationship between THg concentrations and the morphology of perch liver as a main site of metal storage and toxicity. Yellow perch were sampled from five lakes known to contain fish representing a wide range in Hg concentrations in fall 2013. The ultrastructure of hepatocytes and the distribution of Hg within the liver parenchyma were analyzed by transmission electron microscopy (TEM) and electron energy loss spectrometry (EELS). The relative area of macrophage aggregates (MAs) in the liver was determined using image analysis software and fluorescence microscopy. No relation between general health indicators (Fulton's condition index) and THg was observed. In line with this, TEM examination of the liver ultrastructure revealed no prominent pathologies related to THg accumulation. However, a morphological parameter that appeared to increase with muscle THg was the relative area of MAs in the liver. The hepatic lysosomes appeared to be enlarged in samples with the highest THg concentrations. Interestingly, EELS analysis revealed that the MAs and hepatic lysosomes contained Hg.
Evolution of the locomotory system in eels (Teleostei: Elopomorpha).

PubMed

Pfaff, Cathrin; Zorzin, Roberto; Kriwet, Jürgen

2016-08-11

Living anguilliform eels represent a distinct clade of elongated teleostean fishes inhabiting a wide range of habitats. Locomotion of these fishes is highly influenced by the elongated body shape, the anatomy of the vertebral column, and the corresponding soft tissues represented by the musculotendinous system. Up to now, the evolution of axial elongation in eels has been inferred from living taxa only, whereas the reconstruction of evolutionary patterns and functional ecology in extinct eels still is scarce. Rare but excellently preserved fossil eels from the Late Cretaceous and Cenozoic were investigated here to gain a better understanding of locomotory system evolution in anguilliforms and, consequently, their habitat occupations in deep time. The number of vertebrae in correlation with the body length separates extinct and extant anguilliforms. Even if the phylogenetic signal cannot entirely be excluded, the analyses performed here reveal a continuous shortening of the vertebral column with a simultaneous increase in vertebral numbers in conjunction with short lateral tendons throughout the order. These anatomical changes contradict previous hypotheses based on extant eels solely. The body curvatures of extant anguilliforms are highly flexible and can be clearly distinguished from extinct species. Anatomical changes of the vertebral column and musculotendinous system through time and between extinct and extant anguilliforms correlate with changes of the body plan and swimming performance and reveal significant shifts in habitat adaptation and thus behaviour. Evolutionary changes in the skeletal system of eels established here also imply that environmental shifts were triggered by abiotic rather than biotic factors (e.g., K/P boundary mass extinction event).
A review of the possible impacts of long-term oceanic and climate changes and fishing mortality on recruitment of anguillid eels of the Northern Hemisphere.

PubMed

Knights, B

2003-07-01

Possible causes of declines in recruitment of European, American and Japanese eels to continental waters are reviewed. Negative correlations between the Den Oever glass eel recruitment index (DOI) and the North Atlantic Oscillation Index since 1938 are discussed, together with older anecdotal evidence. Correlations are established between the DOI and sea surface temperature anomalies at 100-250 m between 1952 and 1995 in the Sargasso Sea/Sub-Tropical Gyre (STG) spawning area. It is hypothesised that, associated with global warming trends, STG warming inhibits spring thermocline mixing and nutrient circulation, with negative impacts on productivity and hence food for leptocephalus larvae. Concurrent gyre spin-up also affects major currents and slowing of oceanic migration has probably enhanced starvation and predation losses. Local factors, such as unfavourable wind-driven currents, can also affect recruitment of glass eels on continental shelves. In contrast, evidence is discussed that indicates fishing mortality and continental climate change appear to have had lesser impacts. Similar starvation-advection explanations for declines in Japanese eel recruitment are proposed. Predictions for the future are made and multidisciplinary and integrated monitoring and research are recommended for managing eel stocks and fisheries.
Development of guidelines for permitted left-turn phasing using flashing yellow arrows : final report.

DOT National Transportation Integrated Search

2015-06-01

The objective of this project was to develop guidelines for time-of-day use of permitted left-turn phasing, : which can then be implemented using flashing yellow arrows (FYA). This required determining how the risk : for left-turn crashes varied as t...
Regional Variations in the Risk and Severity of Ciguatera Caused by Eating Moray Eels

PubMed Central

Chan, Thomas Y. K.

2017-01-01

Moray eels (Gymnothorax species) from tropical waters have long been known to be high-risk species, and the consumption of particularly the viscera or ungutted eels can result in severe ciguatera (known as Gymnothorax or moray eel poisoning), characterized by prominent neurological features. In this review, the main objective was to describe the risk and severity of ciguatera caused by eating moray eels in different parts of the world. Moray eels can accumulate very high ciguatoxin (CTX) levels in the flesh and particularly the liver. Therefore, even the smaller ones can be toxic and the consumption of an average portion (particularly liver) can result in severe or fatal ciguatera. Moray eels (particularly when ungutted) must never be served in gatherings since they can cause mass poisoning because of their large sizes and high CTX levels. Apart from regulatory measures restricting or excluding access, the public should be repeatedly warned to avoid eating moray eels. PMID:28672845
Regional Variations in the Risk and Severity of Ciguatera Caused by Eating Moray Eels.

PubMed

Chan, Thomas Y K

2017-06-26

Moray eels ( Gymnothorax species) from tropical waters have long been known to be high-risk species, and the consumption of particularly the viscera or ungutted eels can result in severe ciguatera (known as Gymnothorax or moray eel poisoning), characterized by prominent neurological features. In this review, the main objective was to describe the risk and severity of ciguatera caused by eating moray eels in different parts of the world. Moray eels can accumulate very high ciguatoxin (CTX) levels in the flesh and particularly the liver. Therefore, even the smaller ones can be toxic and the consumption of an average portion (particularly liver) can result in severe or fatal ciguatera. Moray eels (particularly when ungutted) must never be served in gatherings since they can cause mass poisoning because of their large sizes and high CTX levels. Apart from regulatory measures restricting or excluding access, the public should be repeatedly warned to avoid eating moray eels.
Glass eels (Anguilla anguilla) have a magnetic compass linked to the tidal cycle

PubMed Central

Cresci, Alessandro; Paris, Claire B.; Durif, Caroline M. F.; Shema, Steven; Bjelland, Reidun M.; Skiftesvik, Anne Berit; Browman, Howard I.

2017-01-01

The European eel (Anguilla anguilla) has one of the longest migrations in the animal kingdom. It crosses the Atlantic Ocean twice during its life history, migrating between the spawning area in the Sargasso Sea and Europe, where it is widely distributed. The leptocephalus larvae drift with the Gulf Stream and other currents for more than a year and metamorphose into glass eels when they arrive on the continental shelf and move toward coastal areas. The mechanisms underlying glass eel orientation toward the coast and into freshwater systems are poorly known. However, anguillid eels, including the glass eel life stage, have a geomagnetic sense, suggesting the possibility that they use Earth’s magnetic field to orient toward the coast. To test this hypothesis, we used a unique combination of laboratory tests and in situ behavioral observations conducted in a drifting circular arena. Most (98%) of the glass eels tested in the sea exhibited a preferred orientation that was related to the tidal cycle. Seventy-one percent of the same eels showed the same orientation during ebb tide when tested in the laboratory under a manipulated simulated magnetic field in the absence of any other cue. These results demonstrate that glass eels use a magnetic compass for orientation and suggest that this magnetic orientation system is linked to a circatidal rhythm. PMID:28630895
Adoption of radio-frequency identification to establish traceability in Taiwanese eel exported to the Japanese market.

PubMed

Jeng, Shu-Ching; Wu, Chun-Lung; Yang, I-Da

2013-01-01

Eel culture and export to the Japanese market is an important industry in Taiwan; however, the average amount produced by each farm is small. Eels from different farms might be mixed before export, making it difficult to determine which farm is responsible for eels containing drug residues. Therefore, the Taiwanese government uses a two-stage procedure of inspection and accreditation for validating the use of good practice in aquaculture farming. Nevertheless, it is still difficult to trace any farm that has produced eels containing drug residues. Radio-frequency identification has the potential to establish traceability in eel products. Here we suggest that Japanese eel importers should insist on the use of radio-frequency identification by Taiwanese eel exporters to enable verification of the safety of eel products being exported to the Japanese market.
Responses of shortfin eel (Anguilla australis) exposed in situ to pulp and paper effluent.

PubMed

van den Heuvel, Michael R; Landman, Michael J; Tremblay, Louis A

2006-10-01

The responses of shortfin eel (Anguilla australis) to discharges from two pulp and paper mills, municipal wastewater, and a geothermal power plant wastewater were examined. Eels were caged at 3 sites along the Tarawera River, North Island, New Zealand, to explore effects of a 3-wk exposure down a contamination gradient (Ref --> D1 --> D2). Most of the observed effects were seen in eels caged at the furthest downstream site (D2), below all the discharge areas. General hematology in eels was unaffected, as measures did not differ markedly at the two downstream sites compared with the reference site. At D2, eels were significantly lighter per unit length (reduced condition factor), although liver and spleen size (LSI and SSI) were unaffected. Significantly elevated circulating sex steroid concentrations (testosterone and estradiol) were measured in D2 eels and increasing sex steroid levels at both sites downstream of the reference site were observed. Significant ethoxyresorufin O-deethylase (EROD) activity induction was seen in D2 eels and bile chemistry showed significant accumulation of pyrene and retene equivalents. However, significantly greater concentrations of total resin acids were found in the bile of eels from the intermediate site (D1), between the two pulp and paper mills. The higher polycyclic aromatic hydrocarbon (PAH) equivalents found in the bile of D2 eels suggest that resin acid neutrals, particularly retene, are responsible for some of the effects observed in eels at the furthest downstream exposure site. Levels of pulp and paper mill extractives in sediment, including the PAH retene, support this conclusion.
Interactions of 2,4,6-trinitrotoluene (TNT) with xenobiotic biotransformation system in European eel Anguilla anguilla (Linnaeus, 1758).

PubMed

Della Torre, Camilla; Corsi, Ilaria; Arukwe, Augustine; Valoti, Massimo; Focardi, Silvano

2008-11-01

The aim of the present study was to investigate the interaction of 2,4,6-trinitrotoluene (TNT) with liver biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758). Eels were exposed to 0.5, 1 and 2.5mg/l nominal concentrations of TNT for 6 and 24h. Modulation of CYP1A1, UDPGT and GST genes was investigated by real-time PCR. Total CYP450 content, NADPH cytochrome c reductase activity, CYP1A and CYP2B-like activities, such as EROD, MROD and BROD, as well as GST and UDPGT activities, were measured by biochemical assays. An in vitro study was performed on EROD in order to evaluate catalytic modulation by TNT. No modulation of the CYP1A1 gene or protein was observed in TNT-exposed eels. On the other hand, a significant decline of EROD and MROD activities was observed in vivo. An increase in NADPH cyt c reductase, and phase II enzymes (UDPGT and GST) were observed at both gene expression and activity levels. The overall results indicated that TNT is a potential competitive inhibitor of CYP1A activities. A TNT metabolic pathway involving NADPH cyt c reductase and phase II enzymes is also suggested.
Compressive Classification for TEM-EELS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hao, Weituo; Stevens, Andrew; Yang, Hao

Electron energy loss spectroscopy (EELS) is typically conducted in STEM mode with a spectrometer, or in TEM mode with energy selction. These methods produce a 3D data set (x, y, energy). Some compressive sensing [1,2] and inpainting [3,4,5] approaches have been proposed for recovering a full set of spectra from compressed measurements. In many cases the final form of the spectral data is an elemental map (an image with channels corresponding to elements). This means that most of the collected data is unused or summarized. We propose a method to directly recover the elemental map with reduced dose and acquisitionmore » time. We have designed a new computational TEM sensor for compressive classification [6,7] of energy loss spectra called TEM-EELS.« less
EELS characterisation of bulk CaCu3Ti4O12 ceramics.

PubMed

Calvert, Clair C; Rainforth, W Mark; Sinclair, Derek C; West, Anthony R

2006-01-01

CaCu3Ti4O12 (CCTO) is a cubic perovskite phase and sintered ceramics exhibit high permittivity at room temperature. Electron energy-loss spectroscopy (EELS) and energy dispersive X-ray spectrometry (EDS) data have been collected from samples of CCTO to relate the observed electrical properties to the microstructure and chemistry on the nanoscale. CCTO ceramics were sintered for 24h at 1115 degrees C in air, giving a grain size of 50-300 microm. Ti L(2,3)-, Cu L(2,3)- and O K-edge EEL data were collected for bulk CCTO (within grain) and compared with well characterised Ti-oxides, CaTiO3 and BaTiO3 perovskites. The bulk metal L(2,3)-edge data for CCTO suggest that Cu is divalent and Ti is present as Ti4+. The O K-edge of CCTO shows increased near-edge structure (NES) compared to those of the simple perovskites.
Isolation of heat-tolerant myoglobin from Asian swamp eel Monopterus albus.

PubMed

Chotichayapong, Chatrachatchaya; Wiengsamut, Kittipong; Chanthai, Saksit; Sattayasai, Nison; Tamiya, Toru; Kanzawa, Nobuyuki; Tsuchiya, Takahide

2012-10-01

Myoglobin from Asian swamp eel Monopterus albus was purified from fish muscle using salt fractionation followed by column chromatography and molecular filtration. The purified Mb of 0.68 mg/g wet weight of muscle was determined for its molecular mass by MALDI-TOF-MS to be 15,525.18 Da. Using isoelectric focusing technique, the purified Mb showed two derivatives with pI of 6.40 and 7.12. Six peptide fragments of this protein identified by LC-MS/MS were homologous to Mbs of sea raven Hemitripterus americanus, yellowfin tuna Thunnus albacores, blue marlin Makaira nigicans, common carp Cyprinus carpio, and goldfish Carassius auratus. According to the Mb denaturation, the swamp eel Mb had thermal stability higher than walking catfish Clarias batrachus Mb and striped catfish Pangasius hypophthalmus Mb, between 30 and 60 (°)C. For the thermal stability of Mb, the swamp eel Mb showed a biphasic behavior due to the O(2) dissociation and the heme orientation disorder, with the lowest increase in both Kd(f) and Kd(s). The thermal sensitivity of swamp eel Mb was lower than those of the other Mbs for both of fast and slow reaction stages. These results suggest that the swamp eel Mb globin structure is thermally stable, which is consistent with heat-tolerant behavior of the swamp eel particularly in drought habitat.

Electric eels use high-voltage to track fast-moving prey

PubMed Central

Catania, Kenneth C.

2015-01-01

Electric eels (Electrophorus electricus) are legendary for their ability to incapacitate fish, humans, and horses with hundreds of volts of electricity. The function of this output as a weapon has been obvious for centuries but its potential role for electroreception has been overlooked. Here it is shown that electric eels use high-voltage simultaneously as a weapon and for precise and rapid electrolocation of fast-moving prey and conductors. Their speed, accuracy, and high-frequency pulse rate are reminiscent of bats using a ‘terminal feeding buzz' to track insects. Eel's exhibit ‘sensory conflict' when mechanosensory and electrosensory cues are separated, striking first toward mechanosensory cues and later toward conductors. Strikes initiated in the absence of conductors are aborted. In addition to providing new insights into the evolution of strongly electric fish and showing electric eels to be far more sophisticated than previously described, these findings reveal a trait with markedly dichotomous functions. PMID:26485580
Lost trust: a yellow fever patient response.

PubMed

Runge, John S

2013-12-13

In the 19th century, yellow fever thrived in the tropical, urban trade centers along the American Gulf Coast. Industrializing and populated, New Orleans and Memphis made excellent habitats for the yellow fever-carrying Aedes aegypti mosquitoes and the virulence they imparted on their victims. Known for its jaundice and black, blood-filled vomit, the malady terrorized the region for decades, sometimes claiming tens of thousands of lives during the near annual summertime outbreaks. In response to the failing medical community, a small, pronounced population of sick and healthy laypeople openly criticized the efforts to rid the Gulf region of yellow jack. Utilizing newspapers and cartoons to vocalize their opinions, these critics doubted and mocked the medical community, contributing to the regional and seasonal dilemma yellow fever posed for the American South. These sentient expressions prove to be an early example of patient distrust toward caregivers, a current problem in clinical heath care.
Lost Trust: A Yellow Fever Patient Response

PubMed Central

Runge, John S.

2013-01-01

In the 19th century, yellow fever thrived in the tropical, urban trade centers along the American Gulf Coast. Industrializing and populated, New Orleans and Memphis made excellent habitats for the yellow fever-carrying Aedes aegypti mosquitoes and the virulence they imparted on their victims. Known for its jaundice and black, blood-filled vomit, the malady terrorized the region for decades, sometimes claiming tens of thousands of lives during the near annual summertime outbreaks. In response to the failing medical community, a small, pronounced population of sick and healthy laypeople openly criticized the efforts to rid the Gulf region of yellow jack. Utilizing newspapers and cartoons to vocalize their opinions, these critics doubted and mocked the medical community, contributing to the regional and seasonal dilemma yellow fever posed for the American South. These sentient expressions prove to be an early example of patient distrust toward caregivers, a current problem in clinical heath care. PMID:24348220
Proximate content of wild and cultured eel (Anguilla bicolor) in different part of body

NASA Astrophysics Data System (ADS)

Wijayanti, I.; Susilo, E. S.

2018-02-01

Proximate content in fish varies depends on intrinsic and extrinsic factors. Intrinsic factors include species, sexual maturity, size and body parts. Extrinsic factors include habitat, season and type of food (diet). This study aimed to know the effect of fish body parts (intrinsic factor) on proximate levels in wild and cultured eel (extrinsic). The experimental design used factorial completely randomized design with two factors 2x3. The first factor is the habitat of eel (wild and cultured) and the second factor is the part of the body (head, body and tail) with five replications. The result of statistical analysis showed that there was interaction between fish habitat and body part on moisture, protein, ash and carbohydrate content (P <0.05), but no interaction on fat content and energy (P> 0.05). The highest water content (67.02%) was found in head of wild and the lowest one (59.44%) in the tail of wild eel; The highest protein content (18.09%) was found in the body of cultured eel and the lowest one (15.72%) was in the body of wild eel; The highest ash content (3.73%) was the head of wild eel and the lowest (1.32%) was in the body of cultured eel; The highest carbohydrate (3.73%) was found in the head of cultured eel and the lowest one (0.16%) was in the body of cultured. The wild eel had higher fat content and energy than cultured one, while the fat content and energy in body and tail were higher than in head.
Impact of long-term habitat loss on the Japanese eel Anguilla japonica

NASA Astrophysics Data System (ADS)

Chen, Jian-Ze; Huang, Shiang-Lin; Han, Yu-San

2014-12-01

Since the 1970s, the loss of temperate-zone anguillid eels, particularly Anguilla anguilla, Anguilla rostrata, and Anguilla japonica, has exceeded 90% based on estimates of glass eel recruitment. The cause of this decline has not been conclusively determined, although many factors have been proposed. In East Asia, the consequences of long-term habitat loss and deterioration of habitat quality on the sustainability of Japanese eel resources are important. Impacts have already occurred and are expected to increase because hundreds of millions of people live near estuaries and rivers that have undergone, and further, are expected to continue to undergo, substantial changes in land use. Driven by economic growth, these landscape changes have resulted in, and may continue to produce, the large-scale destruction of eel habitats. We used chronological Landsat imagery to measure Japanese eel habitat reduction from human activities in 16 rivers in East Asia, including Japan, Korea, Taiwan, and China. On average, 76.8% of the effective habitat area (Ae) was lost in these 16 rivers from the 1970s-2010s. Taiwan and China had the highest percentages of Ae loss, with declines of 49.3% and 81.5%, respectively. Extensive habitat loss may play an important role, together with regional climate phenomena such as the ENSO and overfishing, in the decline of the Japanese eel in East Asia. Measures targeting habitat restoration and protection may need to be integrated into management planning for Japanese eel resources in an international rather than regional context.
Individual movements and population density estimates for moray eels on a Caribbean coral reef

NASA Astrophysics Data System (ADS)

Abrams, R. W.; Schein, M. W.

1986-12-01

Observations of moray eel (Muraenidae) distribution made on a Caribbean coral reef are discussed in the context of long term population trends. Observations of eel distribution made using SCUBA during 1978, 1979 1980, and 1984 are compared and related to the occurrence of a hurricane in 1979. An estimate of the mean standing stock of moray eels is presented. The degree of site attachment is discussed for spotted morays ( Gymnothorax moringa) and goldentail morays ( Muraena miliaris). The repeated non-aggressive association of moray eels with large aggregations of potential prey fishes is detailed.
Introgressive hybridization and latitudinal admixture clines in North Atlantic eels

PubMed Central

2014-01-01

Background Hybridization, the interbreeding of diagnosably divergent species, is a major focus in evolutionary studies. Eels, both from North America and Europe migrate through the Atlantic to mate in a vast, overlapping area in the Sargasso Sea. Due to the lack of direct observation, it is unknown how these species remain reproductively isolated. The detection of inter-species hybrids in Iceland suggests on-going gene flow, but few studies to date have addressed the influence of introgression on genetic differentiation in North Atlantic eels. Results Here, we show that while mitochondrial lineages remain completely distinct on both sides of the Atlantic, limited hybridization is detectable with nuclear DNA markers. The nuclear hybridization signal peaks in the northern areas and decreases towards the southern range limits on both continents according to Bayesian assignment analyses. By simulating increasing proportions of both F1 hybrids and admixed individuals from the southern to the northern-most locations, we were able to generate highly significant isolation-by-distance patterns in both cases, reminiscent of previously published data for the European eel. Finally, fitting an isolation-with-migration model to our data supports the hypothesis of recent asymmetric introgression and refutes the alternative hypothesis of ancient polymorphism. Conclusions Fluctuating degrees of introgressive hybridization between Atlantic eel species are sufficient to explain temporally varying correlations of geographic and genetic distances reported for populations of the European eel. PMID:24674242
Clustering analysis strategies for electron energy loss spectroscopy (EELS).

PubMed

Torruella, Pau; Estrader, Marta; López-Ortega, Alberto; Baró, Maria Dolors; Varela, Maria; Peiró, Francesca; Estradé, Sònia

2018-02-01

In this work, the use of cluster analysis algorithms, widely applied in the field of big data, is proposed to explore and analyze electron energy loss spectroscopy (EELS) data sets. Three different data clustering approaches have been tested both with simulated and experimental data from Fe 3 O 4 /Mn 3 O 4 core/shell nanoparticles. The first method consists on applying data clustering directly to the acquired spectra. A second approach is to analyze spectral variance with principal component analysis (PCA) within a given data cluster. Lastly, data clustering on PCA score maps is discussed. The advantages and requirements of each approach are studied. Results demonstrate how clustering is able to recover compositional and oxidation state information from EELS data with minimal user input, giving great prospects for its usage in EEL spectroscopy. Copyright © 2017 Elsevier B.V. All rights reserved.
Dietary effects on fatty acid composition in muscle tissue of juvenile European eel, Anguilla anguilla (L.)

NASA Astrophysics Data System (ADS)

Prigge, Enno; Malzahn, Arne M.; Zumholz, Karsten; Hanel, Reinhold

2012-03-01

The role of intracontinental migration patterns of European eel ( Anguilla anguilla) receives more and more recognition in both ecological studies of the European eel and possible management measures, but small-scale patterns proved to be challenging to study. We experimentally investigated the suitability of fatty acid trophic markers to elucidate the utilization of feeding habitats. Eight groups of juvenile European eels were fed on eight different diets in a freshwater recirculation system at 20°C for 56 days. Three groups were fed on freshwater diets ( Rutilus rutilus, Chironomidae larvae, and Gammarus pulex) and four groups were reared on diets of a marine origin ( Clupea harengus, Crangon crangon, Mysis spec., and Euphausia superba) and one on commercial pellets used in eel aquaculture. Fatty acid composition (FAC) of diets differed significantly with habitat. FAC of eel muscle tissue seemed to be rather insensitive to fatty acids supplied with diet, but the general pattern of lower n3:n6 and EPA:ARA ratios in freshwater prey organisms could be traced in the respective eels. Multivariate statistics of the fatty acid composition of the eels resulted in two distinct groups representing freshwater and marine treatments. Results further indicate the capability of selectively restraining certain fatty acids in eel, as e.g. the n3:n6 ratio in all treatments was <4, regardless of dietary n3:n6. In future studies on wild eel, these measures can be used to elucidate the utilization of feeding habitats of individual European eel.
7 CFR 28.442 - Middling Yellow Stained Color.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 2 2014-01-01 2014-01-01 false Middling Yellow Stained Color. 28.442 Section 28.442... Stained Color. Middling Yellow Stained Color is American Upland cotton which in color is deeper than Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] below color grade cotton ...
7 CFR 28.442 - Middling Yellow Stained Color.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 2 2012-01-01 2012-01-01 false Middling Yellow Stained Color. 28.442 Section 28.442... Stained Color. Middling Yellow Stained Color is American Upland cotton which in color is deeper than Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] below color grade cotton ...
7 CFR 28.442 - Middling Yellow Stained Color.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 2 2011-01-01 2011-01-01 false Middling Yellow Stained Color. 28.442 Section 28.442... Stained Color. Middling Yellow Stained Color is American Upland cotton which in color is deeper than Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] below color grade cotton ...
7 CFR 28.442 - Middling Yellow Stained Color.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 2 2013-01-01 2013-01-01 false Middling Yellow Stained Color. 28.442 Section 28.442... Stained Color. Middling Yellow Stained Color is American Upland cotton which in color is deeper than Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] below color grade cotton ...
7 CFR 28.442 - Middling Yellow Stained Color.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 2 2010-01-01 2010-01-01 false Middling Yellow Stained Color. 28.442 Section 28.442... Stained Color. Middling Yellow Stained Color is American Upland cotton which in color is deeper than Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] below color grade cotton ...
Tidal-Flat Macrobenthos as Diets of the Japanese Eel Anguilla japonica in Western Japan, with a Note on the Occurrence of a Parasitic Nematode Heliconema anguillae in Eel Stomachs.

PubMed

Kan, Kotaro; Sato, Masanori; Nagasawa, Kazuya

2016-02-01

Dietary items of the Japanese eel Anguilla japonica inhabiting estuaries were examined by analyses of the gut (stomach and intestine) contents in two areas in Kyushu, western Japan. In a small estuary in Kagoshima Bay, where seven eel guts were examined, almost all of the dietary organisms consisted of Hemigrapsus crabs and Hediste polychaetes, both of which commonly occurred on tidal flats of this site during our survey on the macrobenthic fauna. In another large estuary in the innermost part of the Ariake Sea, where 14 eel guts were examined, 11 macrobenthic species (nine crustaceans, a polychaete, and a gastropod) were found in the gut contents, mostly consisting of mudflat-specific species. The dietary items are almost completely different not only between the two estuaries, but also among three neighboring sites within the large estuary in the Ariake Sea. These results show that Japanese eels feed on various macrobenthic invertebrates inhabiting estuarine tidal flats at each site. The variety of the prey species occupying different habitats indicates that their foraging areas extend to a wide range of estuarine tidal flats from the upper to lower littoral zones. The physalopterid nematode Heliconema anguillae was found parasitic in eel stomachs in both estuaries. The prevalence of the nematode was higher in the estuary in Kagoshima Bay (100%) than that in the Ariake Sea (43%), although the intensity in the former (4-94 nematodes per infected stomach) was comparable to that of the latter (2-96). The relationship between the nematode infection and the dietary items of Japanese eels is discussed.
Changes in the role of the thyroid axis during metamorphosis of the Japanese eel, Anguilla japonica.

PubMed

Sudo, Ryusuke; Okamura, Akihiro; Kuroki, Mari; Tsukamoto, Katsumi

2014-08-01

To clarify the role of thyroid function during metamorphosis from leptocephalus to glass eel in the Japanese eel, we examined the histology of the thyroid gland and measured whole-body concentrations of thyroid hormones, thyroxine (T4) and triiodothyronine (T3), and thyroid stimulating hormone β-subunit TSH (TSHβ) mRNA expression levels in five stages of artificially hatched eels (leptocephalus, early-metamorphosis, late-metamorphosis, glass eel, and elver). During metamorphosis, the inner colloid of thyroid follicles showed positive immunoreactivity for T4, and both T4 and T3 levels were significantly increased, whereas a small peak of TSHβ mRNA level was observed at the early-metamorphosis stage. Similarly, TSHβ mRNA levels were highest in the glass eel stage, and then decreased markedly in the elver stage. In contrast to TSHβ mRNA expression, thyroid hormones (both T4 and T3) increased further from the glass eel to elver stages. These results indicated that thyroid function in the Japanese eel was active both during and after metamorphosis. Therefore, the thyrotropic axis may play important roles not only in metamorphosis but also in subsequent inshore or upstream migrations. © 2014 Wiley Periodicals, Inc.
Simultaneous Preconcentration and Determination of Brilliant Blue and Sunset Yellow in Foodstuffs by Solid-Phase Extraction Combined UV-Vis Spectrophotometry.

PubMed

Bişgin, Abdullah Taner

2018-05-29

Background: Brilliant Blue and Sunset Yellow, two highly water-soluble synthetic food dyes, are the most popular food dyes used and consumed. Although they are not highly toxic, some health problems can be observed when excessive amounts of food products containing these dyes are consumed. Objectives: The aim of the study was to develop a simultaneous UV-Vis combined solid-phase extraction method, based on the adsorption onto Amberlite XAD-8 resin, for determination of Brilliant Blue and Sunset Yellow dyes. Methods: Sample solution was poured into the reservoir of the column and permitted to gravitationally pass through the column at 2 mL/min flow rate. Adsorbed dyes were eluted to 5 mL of final volume with 1 mol/L HNO₃ in ethanol solution by applying a 2 mL/min flow rate. Dye concentrations of the solution were determined at 483 and 630 nm for Sunset Yellow and Brilliant Blue, respectively. Results: The detection limits of the method for Brilliant Blue and Sunset Yellow were determined as 0.13 and 0.66 ng/mL, respectively. Preconcentration factor was 80. Brilliant Blue contents of real food samples were found to be between 11 and 240 μg/g. Sunset Yellow concentrations of foodstuffs were determined to be between 19 and 331 μg/g. Conclusions: Economical, effective, and simple simultaneous determination of Brilliant Blue and Sunset Yellow was achieved by using a solid-phase extraction combined UV-Vis spectrometry method. Highlights: The method is applicable and suitable for routine analysis in quality control laboratories without the need for expert personnel and high operational costs because the instrumentation is simple and inexpensive.
Salinity-dependent in vitro effects of homologous natriuretic peptides on the pituitary-interrenal axis in eels.

PubMed

Ventura, Albert; Kusakabe, Makoto; Takei, Yoshio

2011-08-01

We examined the effects of atrial, B-type, ventricular and C-type natriuretic peptides (ANP, BNP, VNP and CNP1, 3, 4) on cortisol secretion from interrenal tissue in vitro in both freshwater (FW) and seawater (SW)-acclimated eels. We first localized the interrenal and chromaffin cells in the eel head kidney using cell specific markers (cholesterol side-chain cleavage enzyme (P450ssc) and tyrosine hydroxylase (TH), respectively) and established the in vitro incubation system for eel interrenal tissue. Unexpectedly, none of the NPs given alone to the interrenal tissue of FW and SW eels stimulated cortisol secretion. However, ANP and VNP, but not BNP and three CNPs, enhanced the steroidogenic action of ACTH in SW interrenal preparations, while CNP1 and CNP4, but not ANP, BNP, VNP and CNP3, potentiated the ACTH action in FW preparations. These salinity dependent effects of NPs are consistent with the previous in vivo study in the eel where endogenous ACTH can act with the injected NPs. 8-Br-cGMP also enhanced the ACTH action in both FW and SW eel preparations, suggesting that the NP actions were mediated by the guanylyl cyclase-coupled NP receptors (GC-A and B) that were localized in the eel interrenal. Further, ANP and CNP1 stimulated ACTH secretion from isolated pituitary glands of SW and/or FW eels. In summary, the present study revealed complex mechanisms of NP action on corticosteroidogenesis through the pituitary-interrenal axis in eels, thereby providing a deeper insight into the role of the NP family in the acclimation of this euryhaline teleost to diverse salinity environments. Copyright © 2011 Elsevier Inc. All rights reserved.
Migratory behavior, metabolism, oxidative stress and mercury concentrations in marine and estuarine European glass eels (Anguilla anguilla).

PubMed

Bolliet, Valérie; Claveau, Julie; Jarry, Marc; Gonzalez, Patrice; Baudrimont, Magalie; Monperrus, Mathilde

2017-02-01

The relationships between the migratory behavior, methylmercury (MeHg) concentrations, oxidative stress response and detoxification processes were investigated in glass eels collected in marine (Molliets) and estuarine (Urt) waters (Adour estuary, South West France) at the end of the fishing season (April). Glass eel migratory behavior was investigated in an experimental flume according to their response to dusk. Fish responding to the decrease in light intensity by ascending in the water column and moving with or against the flow were considered as having a high propensity to migrate (migrant). Glass eels still sheltering at the end of the 24h catching period were considered as having a low propensity to migrate and were called non-migrant. Our results provide some evidence that estuarine glass eels were bigger, presented a higher propensity to migrate and a lower oxidative stress response than marine glass eels. This might reflect a selection process, some marine glass eels progressively settling or dying before reaching Urt and/or a change in feeding behavior. In April, glass eels restart feeding in the Adour estuary which might decrease the oxidative stress possibly related to starvation, and enhance migration. MeHg concentrations was significantly higher in non-migrant than in migrant glass eels and it is suggested that non-migrant glass eels might present a higher vulnerability to stress (at least contamination and/or starvation), although the underlying mechanisms remain to be elucidated. Copyright © 2016 Elsevier Inc. All rights reserved.
PAH Metabolites in Bile of European Eel (Anguilla anguilla) from Morocco.

PubMed

Wariaghli, Fatima; Kammann, Ulrike; Hanel, Reinhold; Yahyaoui, Ahmed

2015-12-01

Environmental pollution of fish with organic contaminants is a topic of rising attention in Morocco. Polycyclic aromatic hydrocarbons (PAH) are prominent organic contaminants which are rapidly metabolized in fish. Their metabolites are accumulated in the bile fluid and can be used to assess PAH exposure. The two PAH metabolites 1-hydroxypyrene and 1-hydroxyphenanthrene were quantified in European eels (Anguilla anguilla) from two Moroccan river systems by high-performance liquid chromatography with fluorescence detection. Mean values ranged from 52 to 210 ng/mL 1-hydroxypyrene and from 61 to 73 ng/mL 1-hydroxyphenanthrene. The overall concentrations of PAH metabolites in eel from Morocco appeared moderate compared to eel from European rivers and coastal sites. The present study provides first information on concentrations of PAH metabolites in fish from Morocco.

A question of origin: dioxin-like PCBs and their relevance in stock management of European eels.

PubMed

Freese, Marko; Sühring, Roxana; Pohlmann, Jan-Dag; Wolschke, Hendrik; Magath, Victoria; Ebinghaus, Ralf; Hanel, Reinhold

2016-01-01

The stock of European Eel (Anguilla anguilla L.) has reached an all-time low in 2011. Spawner quality of mature eels in terms of health status and fitness is considered one of the key elements for successful migration and reproduction. Dioxin-like Polychlorinated Biphenyls (dl-PCBs) are known persistent organic pollutants potentially affecting the reproductive capability and health status of eels throughout their entire lifetime. In this study, muscle tissue samples of 192 European eels of all continental life stages from 6 different water bodies and 13 sampling sites were analyzed for contamination with lipophilic dl-PCBs to investigate the potential relevance of the respective habitat in light of eel stock management. Results of this study reveal habitat-dependent and life history stage-related accumulation of targeted PCBs. Sum concentrations of targeted PCBs differed significantly between life stages and inter-habitat variability in dl-PCB levels and -profiles was observed. Among all investigated life stages, migrant silver eels were found to be the most suitable life history stage to represent their particular water system due to habitat dwell-time and their terminal contamination status. With reference to a possible negative impact of dl-PCBs on health and the reproductive capability of eels, it was hypothesized that those growing up in less polluted habitats have a better chance to produce healthy offspring than those growing up in highly polluted habitats. We suggest that the contamination status of water systems is fundamental for the life cycle of eels and needs to be considered in stock management and restocking programs.
A combination mode of climate variability responsible for extremely poor recruitment of the Japanese eel (Anguilla japonica)

NASA Astrophysics Data System (ADS)

Lin, Yong-Fu; Wu, Chau-Ron; Han, Yu-San

2017-03-01

Satellite data and assimilation products are used to investigate fluctuations in the catch of Japanese eel (Anguilla japonica) in eastern Asian countries. It has been reported that the salinity front has extended farther south, which has shifted the eel’s spawning grounds to a lower latitude, resulting in smaller eel catches in 1983, 1992, and 1998. This study demonstrates that interannual variability in the eel catch is strongly correlated with the combination mode (C-mode), but not with the El Niño-Southern Oscillation. These eels continue to spawn within the North Equatorial Current (NEC), but the salinity front shifts south during a canonical El Niño. On the other hand, the spawning grounds accompanied by the salinity front extend farther south during the C-mode of climate variability, and eel larvae fail to join the nursery in the NEC, resulting in extremely poor recruitment in East Asia. We propose an appropriate sea surface temperature index to project Japanese eel larval catch.
Complete genomic sequence and taxonomic position of eel virus European X (EVEX), a rhabdovirus of European eel.

PubMed

Galinier, Richard; van Beurden, Steven; Amilhat, Elsa; Castric, Jeannette; Schoehn, Guy; Verneau, Olivier; Fazio, Géraldine; Allienne, Jean-François; Engelsma, Marc; Sasal, Pierre; Faliex, Elisabeth

2012-06-01

Eel virus European X (EVEX) was first isolated from diseased European eel Anguilla anguilla in Japan at the end of seventies. The virus was tentatively classified into the Rhabdoviridae family on the basis of morphology and serological cross reactivity. This family of viruses is organized into six genera and currently comprises approximately 200 members, many of which are still unassigned because of the lack of molecular data. This work presents the morphological, biochemical and genetic characterizations of EVEX, and proposes a taxonomic classification for this virus. We provide its complete genome sequence, plus a comprehensive sequence comparison between isolates from different geographical origins. The genome encodes the five classical structural proteins plus an overlapping open reading frame in the phosphoprotein gene, coding for a putative C protein. Phylogenic relationship with other rhabdoviruses indicates that EVEX is most closely related to the Vesiculovirus genus and shares the highest identity with trout rhabdovirus 903/87. Copyright © 2012 Elsevier B.V. All rights reserved.
Sub-decadal turbidite frequency during the early Holocene: Eel Fan, offshore northern California

USGS Publications Warehouse

Paull, Charles K.; McGann, Mary L.; Sumner, Esther J; Barnes, Philip M; Lundsten, Eve M.; Anderson, Krystle; Gwiazda, Roberto; Edwards, Brian D.; Caress, David W

2014-01-01

Remotely operated and autonomous underwater vehicle technologies were used to image and sample exceptional deep sea outcrops where an ∼100-m-thick section of turbidite beds is exposed on the headwalls of two giant submarine scours on Eel submarine fan, offshore northern California (USA). These outcrops provide a rare opportunity to connect young deep-sea turbidites with their feeder system. 14C measurements reveal that from 12.8 ka to 7.9 ka, one turbidite was being emplaced on average every 7 yr. This emplacement rate is two to three orders of magnitude higher than observed for turbidites elsewhere along the Pacific margin of North America. The turbidites contain abundant wood and shallow-dwelling foraminifera, demonstrating an efficient connection between the Eel River source and the Eel Fan sink. Turbidite recurrence intervals diminish fivefold to ∼36 yr from 7.9 ka onward, reflecting sea-level rise and re-routing of Eel River sediments.
Influence of the North Atlantic oceanograghic and climatic parameters on the Spanish European Eel population recruitment: relationships in the past and for a future climate change

NASA Astrophysics Data System (ADS)

Ribalaygua, Jaime; Pórtoles, Javier; Monjo, Robert; Díaz, Estíbaliz; Korta, María; Chust, Guillem

2016-04-01

The status of the European eel population is critical.; the annual recruitment of glass eel to European waters in 2015 is 1.2% of the 1960-1979 level in the 'North Sea' area, and 8.4% in the rest of Europe (ICES 2015) . There are a number of anthropogenic impacts potentially affecting eel population including commercial exploitation, habitat loss, dam and weir construction, hydropower, pumping stations and surface water abstractions. Furthermore, the first eel stages and larval migration and marine survival are heavily influenced by oceanic and climatic factors since the species breeds in the Sargasso Sea and migrates to the continental shelf of the Atlantic coast of Europe and North Africa. Therefore, the study of the relations between recruitment and oceanic conditions may allow to study the potential effect of climatic change on the future eel recruitment and therefore stock. In the present study, the relation between glass eel recruitment and oceanic and climatic factors has been studied. Historic glass eel catches data beginning in the 50s from two Mediterranean and two Atlantic estuaries have been used as a proxy of recruitment. The relation of catches with the main oceanographic and climatic factors identified in the literature was established using an ocean reanalysis, the Simple Ocean Data Assimilation (SODA) and determined which variables are significantly related to the number of catches. The analysis shows significant relationships between catches and oceanic (Surface Downward Stress, Sea Water Temperature and Sea Water Velocity) and atmospheric (NAO Index, AMO Index) variables. Subsequently, we applied the results of three climate models (GFDL-ESM2M, CanESM2 and CNRM-CM5), associated with the Coupled Model Intercomparison Project Phase 5 (CMIP5) under two simulations of climate change (RCP4.5 and RCP8.5), both associated with the 5th Assessment Report of the IPCC, for possible future influences on the eel. This research was funded by the Spanish
Persistence of yellow fever vaccine-induced antibodies after solid organ transplantation.

PubMed

Wyplosz, B; Burdet, C; François, H; Durrbach, A; Duclos-Vallée, J C; Mamzer-Bruneel, M-F; Poujol, P; Launay, O; Samuel, D; Vittecoq, D; Consigny, P H

2013-09-01

Immunization using live attenuated vaccines represents a contra-indication after solid organ transplantation (SOT): consequently, transplant candidates planning to travel in countries where yellow fever is endemic should be vaccinated prior to transplantation. The persistence of yellow fever vaccine-induced antibodies after transplantation has not been studied yet. We measured yellow-fever neutralizing antibodies in 53 SOT recipients vaccinated prior to transplantation (including 29 kidney recipients and 18 liver recipients). All but one (98%) had protective titers of antibodies after a median duration of 3 years (min.: 0.8, max.: 21) after transplantation. The median antibody level was 40 U/L (interquartile range: 40-80). For the 46 patients with a known or estimated date of vaccination, yellow-fever antibodies were still detectable after a median time of 13 years (range: 2-32 years) post-immunization. Our data suggest there is long-term persistence of antibodies to yellow fever in SOT recipients who have been vaccinated prior to transplantation. © Copyright 2013 The American Society of Transplantation and the American Society of Transplant Surgeons.
The 'electric stroke' and the 'electric spark': anatomists and eroticism at George Baker's electric eel exhibition in 1776 and 1777.

PubMed

Plumb, Christopher

2010-09-01

In 1776 and 1777 five living electric eels exhibited in London became a sensational spectacle that appealed to anatomists, electricians and connoisseurs of erotica. George Baker's exhibition made visible the 'electric spark' of the electrical eel and a series of experiments were both witnessed by and participated in by members of the Royal Society and the metropolitan elite. Some participants even grasped the eels firmly in their hands and felt the 'electric stroke' of the eel in addition to observing the spark. In their observation of the electric eel some of these spectators transposed the vivid electric spark from the sphere of electricians and anatomists into that of satirical and erotic literature. Here the erotic electric eel proliferated in the literature and the eel took on quite different connotations that nonetheless were reliant on readers knowledge and experience of the exhibition, experiments and the preoccupations of anatomists. George Baker's electric eel exhibition of 1776 and 1777 is then instructive in exploring the production and circulation of knowledge in Georgian Britain. The story of the electric eel in Georgian culture charts the creation of the electric spark and stroke as objects of observation and encounter, their exhibitionary context, and finally their divergent meanings as the electric eel became erotically charged for a metropolitan masculine elite. (c) 2010 Elsevier Ltd. All rights reserved.
Phylogeny of Amazona barbadensis and the Yellow-headed Amazon complex (Aves: Psittacidae): a new look at South American parrot evolution.

PubMed

Urantówka, Adam Dawid; Mackiewicz, Paweł; Strzała, Tomasz

2014-01-01

The Yellow-shouldered Amazon (Amazona barbadensis) is the sole parrot of the genus Amazona that inhabits only dry forests. Its population has been dropping; therefore it has been the topic of many studies and conservation efforts. However, the phylogenetic relationship of this species to potential relatives classified within the Yellow-Headed Amazon (YHA) complex are still not clear. Therefore, we used more extensive data sets, including the newly sequenced mitochondrial genome of A. barbadensis, to conduct phylogenetic analyses. Various combinations of genes and many phylogenetic approaches showed that A. barbadensis clustered significantly with A. ochrocephala ochrocephala from Colombia and Venezuela, which created the Northern South American (NSA) lineage, clearly separated from two other lineages within the YHA complex, the Central (CA) and South American (SA). Tree topology tests and exclusion of rapidly evolving sites provided support for a NSA+SA grouping. We propose an evolutionary scenario for the YHA complex and its colonization of the American mainland. The NSA lineage likely represents the most ancestral lineage, which derived from Lesser Antillean Amazons and colonized the northern coast of Venezuela about a million years ago. Then, Central America was colonized through the Isthmus of Panama, which led to the emergence of the CA lineage. The southward expansion to South America and the origin of the SA lineage happened almost simultaneously. However, more intensive or prolonged gene flow or migrations have led to much weaker geographic differentiation of genetic markers in the SA than in the CA lineage.
EELS from organic crystalline materials

NASA Astrophysics Data System (ADS)

Brydson, R.; Eddleston, M. D.; Jones, W.; Seabourne, C. R.; Hondow, N.

2014-06-01

We report the use of the electron energy loss spectroscopy (EELS) for providing light element chemical composition information from organic, crystalline pharmaceutical materials including theophylline and paracetamol and discuss how this type of data can complement transmission electron microscopy (TEM) imaging and electron diffraction when investigating polymorphism. We also discuss the potential for the extraction of bonding information using electron loss near-edge structure (ELNES).
Dynamic flashing yellow arrow (FYA): a study on variable left-turn mode operational and safety impacts phase II - model expansion and testing.

DOT National Transportation Integrated Search

2016-06-01

The flashing yellow arrow (FYA) signal display creates an opportunity to enhance the left-turn phase with a : variable mode that can be changed on demand. The previously developed decision support system (DSS) in : phase I facilitated the selection o...
Empirical observations of the spawning migration of European eels: The long and dangerous road to the Sargasso Sea.

PubMed

Righton, David; Westerberg, Håkan; Feunteun, Eric; Økland, Finn; Gargan, Patrick; Amilhat, Elsa; Metcalfe, Julian; Lobon-Cervia, Javier; Sjöberg, Niklas; Simon, Janek; Acou, Anthony; Vedor, Marisa; Walker, Alan; Trancart, Thomas; Brämick, Uwe; Aarestrup, Kim

2016-10-01

The spawning migration of the European eel ( Anguilla anguilla L.) to the Sargasso Sea is one of the greatest animal migrations. However, the duration and route of the migration remain uncertain. Using fishery data from 20 rivers across Europe, we show that most eels begin their oceanic migration between August and December. We used electronic tagging techniques to map the oceanic migration from eels released from four regions in Europe. Of 707 eels tagged, we received 206 data sets. Many migrations ended soon after release because of predation events, but we were able to reconstruct in detail the migration routes of >80 eels. The route extended from western mainland Europe to the Azores region, more than 5000 km toward the Sargasso Sea. All eels exhibited diel vertical migrations, moving from deeper water during the day into shallower water at night. The range of migration speeds was 3 to 47 km day -1 . Using data from larval surveys in the Sargasso Sea, we show that spawning likely begins in December and peaks in February. Synthesizing these results, we show that the timing of autumn escapement and the rate of migration are inconsistent with the century-long held assumption that eels spawn as a single reproductive cohort in the springtime following their escapement. Instead, we suggest that European eels adopt a mixed migratory strategy, with some individuals able to achieve a rapid migration, whereas others arrive only in time for the following spawning season. Our results have consequences for eel management.
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel.

PubMed

Pujolar, Jose Martin; Marino, Ilaria A M; Milan, Massimo; Coppe, Alessandro; Maes, Gregory E; Capoccioni, Fabrizio; Ciccotti, Eleonora; Bervoets, Lieven; Covaci, Adrian; Belpaire, Claude; Cramb, Gordon; Patarnello, Tomaso; Bargelloni, Luca; Bortoluzzi, Stefania; Zane, Lorenzo

2012-09-25

Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel (Anguilla anguilla) stock has been declining since the 1980s probably due to a combination of anthropogenic and climatic factors. In this paper, we explore the transcriptomic dynamics between individuals from high (river Tiber, Italy) and low pollution (lake Bolsena, Italy) environments, which were measured for 36 PCBs, several organochlorine pesticides and brominated flame retardants and nine metals. To this end, we first (i) updated the European eel transcriptome using deep sequencing data with a total of 640,040 reads assembled into 44,896 contigs (Eeelbase release 2.0), and (ii) developed a transcriptomic platform for global gene expression profiling in the critically endangered European eel of about 15,000 annotated contigs, which was applied to detect differentially expressed genes between polluted sites. Several detoxification genes related to metabolism of pollutants were upregulated in the highly polluted site, including genes that take part in phase I of the xenobiotic metabolism (CYP3A), phase II (glutathione-S-transferase) and oxidative stress (glutathione peroxidase). In addition, key genes in the mitochondrial respiratory chain and oxidative phosphorylation were down-regulated at the Tiber site relative to the Bolsena site. Together with the induced high expression of detoxification genes, the suggested lowered expression of genes supposedly involved in metabolism suggests that pollution may also be associated with decreased respiratory and energy production.
Surviving in a toxic world: transcriptomics and gene expression profiling in response to environmental pollution in the critically endangered European eel

PubMed Central

2012-01-01

Background Genomic and transcriptomic approaches have the potential for unveiling the genome-wide response to environmental perturbations. The abundance of the catadromous European eel (Anguilla anguilla) stock has been declining since the 1980s probably due to a combination of anthropogenic and climatic factors. In this paper, we explore the transcriptomic dynamics between individuals from high (river Tiber, Italy) and low pollution (lake Bolsena, Italy) environments, which were measured for 36 PCBs, several organochlorine pesticides and brominated flame retardants and nine metals. Results To this end, we first (i) updated the European eel transcriptome using deep sequencing data with a total of 640,040 reads assembled into 44,896 contigs (Eeelbase release 2.0), and (ii) developed a transcriptomic platform for global gene expression profiling in the critically endangered European eel of about 15,000 annotated contigs, which was applied to detect differentially expressed genes between polluted sites. Several detoxification genes related to metabolism of pollutants were upregulated in the highly polluted site, including genes that take part in phase I of the xenobiotic metabolism (CYP3A), phase II (glutathione-S-transferase) and oxidative stress (glutathione peroxidase). In addition, key genes in the mitochondrial respiratory chain and oxidative phosphorylation were down-regulated at the Tiber site relative to the Bolsena site. Conclusions Together with the induced high expression of detoxification genes, the suggested lowered expression of genes supposedly involved in metabolism suggests that pollution may also be associated with decreased respiratory and energy production. PMID:23009661
Mechanical properties of salvaged dead yellow-cedar in southeast Alaska : Phase I

Treesearch

K. A. McDonald; P. E. Hennon; J. H. Stevens; D. W. Green

An intensive decline and mortality problem is affecting yellow-cedar trees in southeast Alaska. Yellow-cedar snags (dead trees) could be important to the economy in southeast Alaska, if some high-value uses for the snags could be established. Due to the high decay resistance of yellow-cedar, the rate of deterioration is so slow that snags may remain standing for a...
Ubiquitous distribution of fluorescent protein in muscles of four species and two subspecies of eel (genus Anguilla).

PubMed

Funahashi, Aki; Itakura, Takao; Hassanin Abeer, A I; Komatsu, Masaharu; Hayashi, Seiichi; Kaminishi, Yoshio

2017-03-01

In this study, the localization of fluorescent protein (FP) was characterized in the muscles of four species and two subspecies of eels Anguilla anguilla, A. australis, A. bicolor bicolor (b.), A. bicolor pacifica (p.) and A. mossambica in addition to the previously reported A. japonica. The open reading frame of each eel FP was 417 bp encoding 139 amino acid residues. The deduced amino acid sequences among the four species and two subspecies exhibited 91.4-100% identity, and belonged to the fatty-acid-binding protein (FABP) family. The gene structure of eel FPs in A. japonica, A. anguilla, A. australis, A. bicolor b., A. bicolor p. and A. mossambica have four exons and three introns, and were common to that of FABP family. The apo eel FPs expressed by Escherichia coli with recombinant eel FP genes were analysed for the fluorescent properties in the presence of bilirubin. The excitation and emission spectra of holo eel FPs had the maximum wavelengths of 490-496 and 527-530 nm, respectively. The holo eel FPs indicated that the fluorescent intensities were stronger in A. japonica and A. bicolor than in A. mossambica, A. australis and A. anguilla. The comparison of amino acid sequences revealed two common substitutions in A. mossambica, A. australis and A. anguilla with weak fluorescent intensity.
Sexually dimorphic gene expressions in eels: useful markers for early sex assessment in a conservation context

PubMed Central

Geffroy, Benjamin; Guilbaud, Florian; Amilhat, Elsa; Beaulaton, Laurent; Vignon, Matthias; Huchet, Emmanuel; Rives, Jacques; Bobe, Julien; Fostier, Alexis; Guiguen, Yann; Bardonnet, Agnès

2016-01-01

Environmental sex determination (ESD) has been detected in a range of vertebrate reptile and fish species. Eels are characterized by an ESD that occurs relatively late, since sex cannot be histologically determined before individuals reach 28 cm. Because several eel species are at risk of extinction, assessing sex at the earliest stage is a crucial management issue. Based on preliminary results of RNA sequencing, we targeted genes susceptible to be differentially expressed between ovaries and testis at different stages of development. Using qPCR, we detected testis-specific expressions of dmrt1, amh, gsdf and pre-miR202 and ovary-specific expressions were obtained for zar1, zp3 and foxn5. We showed that gene expressions in the gonad of intersexual eels were quite similar to those of males, supporting the idea that intersexual eels represent a transitional stage towards testicular differentiation. To assess whether these genes would be effective early molecular markers, we sampled juvenile eels in two locations with highly skewed sex ratios. The combined expression of six of these genes allowed the discrimination of groups according to their potential future sex and thus this appears to be a useful tool to estimate sex ratios of undifferentiated juvenile eels. PMID:27658729
Sexually dimorphic gene expressions in eels: useful markers for early sex assessment in a conservation context

NASA Astrophysics Data System (ADS)

Geffroy, Benjamin; Guilbaud, Florian; Amilhat, Elsa; Beaulaton, Laurent; Vignon, Matthias; Huchet, Emmanuel; Rives, Jacques; Bobe, Julien; Fostier, Alexis; Guiguen, Yann; Bardonnet, Agnès

2016-09-01

Environmental sex determination (ESD) has been detected in a range of vertebrate reptile and fish species. Eels are characterized by an ESD that occurs relatively late, since sex cannot be histologically determined before individuals reach 28 cm. Because several eel species are at risk of extinction, assessing sex at the earliest stage is a crucial management issue. Based on preliminary results of RNA sequencing, we targeted genes susceptible to be differentially expressed between ovaries and testis at different stages of development. Using qPCR, we detected testis-specific expressions of dmrt1, amh, gsdf and pre-miR202 and ovary-specific expressions were obtained for zar1, zp3 and foxn5. We showed that gene expressions in the gonad of intersexual eels were quite similar to those of males, supporting the idea that intersexual eels represent a transitional stage towards testicular differentiation. To assess whether these genes would be effective early molecular markers, we sampled juvenile eels in two locations with highly skewed sex ratios. The combined expression of six of these genes allowed the discrimination of groups according to their potential future sex and thus this appears to be a useful tool to estimate sex ratios of undifferentiated juvenile eels.
EEL spectroscopic tomography: towards a new dimension in nanomaterials analysis.

PubMed

Yedra, Lluís; Eljarrat, Alberto; Arenal, Raúl; Pellicer, Eva; Cabo, Moisés; López-Ortega, Alberto; Estrader, Marta; Sort, Jordi; Baró, Maria Dolors; Estradé, Sònia; Peiró, Francesca

2012-11-01

Electron tomography is a widely spread technique for recovering the three dimensional (3D) shape of nanostructured materials. Using a spectroscopic signal to achieve a reconstruction adds a fourth chemical dimension to the 3D structure. Up to date, energy filtering of the images in the transmission electron microscope (EFTEM) is the usual spectroscopic method even if most of the information in the spectrum is lost. Unlike EFTEM tomography, the use of electron energy-loss spectroscopy (EELS) spectrum images (SI) for tomographic reconstruction retains all chemical information, and the possibilities of this new approach still remain to be fully exploited. In this article we prove the feasibility of EEL spectroscopic tomography at low voltages (80 kV) and short acquisition times from data acquired using an aberration corrected instrument and data treatment by Multivariate Analysis (MVA), applied to Fe(x)Co((3-x))O(4)@Co(3)O(4) mesoporous materials. This approach provides a new scope into materials; the recovery of full EELS signal in 3D. Copyright © 2012 Elsevier B.V. All rights reserved.
Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus): effect of seawater acclimation.

PubMed

Kalujnaia, Svetlana; Hazon, Neil; Cramb, Gordon

2016-08-01

A single MIPS gene (Isyna1/Ino1) exists in eel and tilapia genomes with a single myo-d-inositol 3-phosphate synthase (MIPS) transcript identified in all eel tissues, although two MIPS spliced variants [termed MIPS(s) and MIPS(l)] are found in all tilapia tissues. The larger tilapia transcript [MIPS(l)] results from the inclusion of the 87-nucleotide intron between exons 5 and 6 in the genomic sequence. In most tilapia tissues, the MIPS(s) transcript exhibits much higher abundance (generally >10-fold) with the exception of white skeletal muscle and oocytes, in which the MIPS(l) transcript predominates. SW acclimation resulted in large (6- to 32-fold) increases in mRNA expression for both MIPS(s) and MIPS(l) in all tilapia tissues tested, whereas in the eel, changes in expression were limited to a more modest 2.5-fold increase and only in the kidney. Western blots identified a number of species- and tissue-specific immunoreactive MIPS proteins ranging from 40 to 67 kDa molecular weight. SW acclimation failed to affect the abundance of any immunoreactive protein in any tissue tested from the eel. However, a major 67-kDa immunoreactive protein (presumed to be MIPS) found in tilapia tissues exhibited 11- and 54-fold increases in expression in gill and fin samples from SW-acclimated fish. Immunohistochemical investigations revealed specific immunoreactivity in the gill, fin, skin, and intestine taken from only SW-acclimated tilapia. Immunofluorescence indicated that MIPS was expressed within gill chondrocytes and epithelial cells of the primary filaments, basal epithelial cell layers of the skin and fin, the cytosol of columnar intestinal epithelial and mucous cells, as well as unknown entero-endocrine-like cells. Copyright © 2016 the American Physiological Society.
When yellow lights look red: tinted sunglasses on the railroads.

PubMed

Hovis, Jeffery K

2011-02-01

A major Canadian railway company purchased safety eyewear sunglasses that were purported to have a neutral gray tint and that met the North American occupational and fashion sunglass requirements for signal light transmittance. After several weeks, the company began to receive reliable reports from employees that the yellow wayside signal appeared red when viewed through these sunglasses. Furthermore, the lenses themselves appeared to have a greenish brown tint rather than gray as the labeling implied. The transmission properties of the lenses were measured with a spectrophotometer, and color shifts were calculated for both roadway and railway signal lights. The lenses did have a brown tint and they did meet the North American and European occupational sunglass transmittance requirements for roadway traffic signal lights. However, they did not meet the Australian occupational requirements because the red signal visibility factor was too high. Calculations using typical railroad wayside signal lights showed that the lenses would shift the yellow signal chromaticity coordinates beyond the boundaries for the railway yellow signals and toward the red end of the International Commission on Illumination chromaticity diagram, confirming the employees' reports. Although the lenses met the North American and European sunglass transmittance requirements for traffic signal lights, the results showed that these standards are inappropriate for the railroad environment because the yellow wayside signal lights are redder and smaller in angular size than typical North American and European traffic lights. Some suggestions on a modified transmittance requirement are given to avoid this problem in the future.

Distribution of Glass Eel by the Water Surface Salinity Using Landsat TM at Pelabuhan Ratu Bay, West Java

NASA Astrophysics Data System (ADS)

Irianto, D. S.; Supriatna; Pin, TjiongGiok

2016-11-01

Eel (Anguilla spp.) is consumed fish that has an important economic value, either for local or international market. Pelabuhanratu Bay is an area with big potential for supplying eel seed. One of important factor, which affect an eel existence, is salinity, because eel migrate from fresh water, brackish, and sea naturally although the otherwise so that need ways to describe the distribution of glass eel by the salinity. To find out the percentage of salinity, it obtained from Landsat 8 Imagery in year 2015 using salinity prediction of Algorithm Cimandiri. The research has been conducted at Cimandiri Estuary, Citepus Estuary, and Cimaja Estuary based on wet and dry months. The existence of glass eel which is obtained from the catch was occurs on dry month when the most catch was occurs at the edge of estuary. The catch is reduced if it's farther from the edge of estuary, at the beach towards the sea and the inside of the river mouth with the percentage of salinity towards the sea is increase while the percentage of salinity towards the river is decrease.
Phylogeny of Amazona barbadensis and the Yellow-Headed Amazon Complex (Aves: Psittacidae): A New Look at South American Parrot Evolution

PubMed Central

Strzała, Tomasz

2014-01-01

The Yellow-shouldered Amazon (Amazona barbadensis) is the sole parrot of the genus Amazona that inhabits only dry forests. Its population has been dropping; therefore it has been the topic of many studies and conservation efforts. However, the phylogenetic relationship of this species to potential relatives classified within the Yellow-Headed Amazon (YHA) complex are still not clear. Therefore, we used more extensive data sets, including the newly sequenced mitochondrial genome of A. barbadensis, to conduct phylogenetic analyses. Various combinations of genes and many phylogenetic approaches showed that A. barbadensis clustered significantly with A. ochrocephala ochrocephala from Colombia and Venezuela, which created the Northern South American (NSA) lineage, clearly separated from two other lineages within the YHA complex, the Central (CA) and South American (SA). Tree topology tests and exclusion of rapidly evolving sites provided support for a NSA+SA grouping. We propose an evolutionary scenario for the YHA complex and its colonization of the American mainland. The NSA lineage likely represents the most ancestral lineage, which derived from Lesser Antillean Amazons and colonized the northern coast of Venezuela about a million years ago. Then, Central America was colonized through the Isthmus of Panama, which led to the emergence of the CA lineage. The southward expansion to South America and the origin of the SA lineage happened almost simultaneously. However, more intensive or prolonged gene flow or migrations have led to much weaker geographic differentiation of genetic markers in the SA than in the CA lineage. PMID:24823658
Addressing the impact of mercury estuarine contamination in the European eel (Anguilla anguilla L., 1758) - An early diagnosis in glass eel stage based on erythrocytic nuclear morphology.

PubMed

Castro, D; Mieiro, C L; Coelho, J P; Guilherme, S; Marques, A; Santos, M A; Duarte, A C; Pereira, E; Pacheco, M

2018-02-01

The decline of the European eel (Anguilla anguilla L., 1758) population throughout Europe has been partially attributed to pollution. As glass eel estuarine migration may represent a considerable threat, the impact of mercury (Hg) contamination at this stage was evaluated through an in situ experiment (7days). Total Hg (tHg) bioaccumulation was evaluated concomitantly with erythrocytic nuclear morphology alterations: erythrocytic nuclear abnormalities assay (ENA), frequency of immature erythrocytes (IE) and the erythrocytic maturity index (EMI). The ENA results suggested a genotoxic pressure at the most contaminated sites, in line with the tHg increase. The EMI data, together with IE frequency, showed that fish exposed to high levels of Hg exhibited alterations of haematological dynamics, translated into an erythropoiesis increment. Despite the presence of these compensatory mechanisms, the present findings suggest a harmful impact of Hg on genome integrity at this early development stage, potentially affecting eels' condition and ultimately the population sustainability. Copyright © 2017. Published by Elsevier Ltd.
Influence of Anguillicola crassus (Nematoda) and Ichthyophthirius multifiliis (Ciliophora) on swimming activity of European eel Anguilla anguilla.

PubMed

Münderle, M; Sures, B; Taraschewski, H

2004-08-09

We investigated the swimming activity of 70 European eels Anguilla anguilla in relation to natural infection with 2 parasite species: the eel-specific swimbladder nematode Anguillicola crassus and the non-specific skin and gill protozoan Ichthyophthirius multifiliis. We measured how long individual eels exposed to a water current in a swimming channel with a steady-stream profile could withstand the water current. The parasites affected the swimming behaviour of eels in different ways. The maximum period of time the fish were able to swim against the current was not correlated with infection by A. crassus. In contrast, infection with I. multifiliis reduced the swimming time. The protozoan has a higher pathogenicity than the swimbladder nematode, at least in closed systems, where I. multifiliis is able to spread within a few days. Reduction in swimming capacity after infection with the ciliate averaged 47 % compared to capacity prior to infection. Thus, our results do not support the previously suggested strong negative relation between swimming activity of eels and intensity of A. crassus infection, at least in the short-term. However, there are indications in the literature that the pathological effects of A. crassus on the eel swimmbladder may involve a higher energy demand, possibly manifested in a prolonged spawning migration. As a result, eels heavily infected with this parasite may arrive too late at the spawning site to participate in mating. This could ensure a selection of 'good genes'.
The risk of urban yellow fever resurgence in Aedes-infested American cities.

PubMed

Massad, Eduardo; Amaku, Marcos; Coutinho, Francisco Antonio Bezerra; Struchiner, Claudio José; Lopez, Luis Fernandez; Coelho, Giovanini; Wilder-Smith, Annelies; Burattini, Marcelo Nascimento

2018-05-30

Aedes aegypti, historically known as yellow fever (YF) mosquito, transmits a great number of other viruses such as Dengue, West Nile, Chikungunya, Zika, Mayaro and perhaps Oropouche, among others. Well established in Africa and Asia, Aedes mosquitoes are now increasingly invading large parts of the American continent, and hence the risk of urban YF resurgence in the American cities should because of great concern to public health authorities. Although no new urban cycle of YF was reported in the Americas since the end of an Aedes eradication programme in the late 1950s, the high number of non-vaccinated individuals that visit endemic areas, that is, South American jungles where the sylvatic cycle of YF is transmitted by canopy mosquitoes, and return to Aedes-infested urban areas, increases the risk of resurgence of the urban cycle of YF. We present a method to estimate the risk of urban YF resurgence in dengue-endemic cities. This method consists in (1) to estimate the number of Aedes mosquitoes that explains a given dengue outbreak in a given region; (2) calculate the force of infection caused by the introduction of one infective individual per unit area in the endemic area under study; (3) using the above estimates, calculate the probability of at least one autochthonous YF case per unit area produced by one single viraemic traveller per unit area arriving from a YF endemic or epidemic sylvatic region at the city studied. We demonstrate that, provided the relative vector competence, here defined as the capacity to being infected and disseminate the virus, of Ae. aegypti is greater than 0.7 (with respect to dengue), one infected traveller can introduce urban YF in a dengue endemic area.
[The South-American eel. Two early letters on animal electricity from the Dutch colonies].

PubMed

Koehler, P J; Finger, S

2008-01-01

Speculations about the nature of nerve action, including animal spirits, date back to antiquity. Only during the 18th century, when it became possible to store electricity in Leyden jars, did natural philosophers begin to realise that the sensations caused by electric fish are like those produced by these primitive capacitors. The important roles played by amateur observers in the Dutch colonies of South-America, and how they communicated with more established Dutch scientists in The Netherlands, are often relatively overlooked in histories of how the nerves became electrical. In this article we examine two mid-18th-century letters from South-America to the Netherlands. Both dealt with what would soon be called the electric eel, and were published in the proceedings of the first Dutch Society of Science (in Haarlem). The discovery of the electric nature of the shocks produced by these fish in particular proved to be a crucial step in understanding animal electricity and the true nature of neurophysiology.
The first success of glass eel production in the world: basic biology on fish reproduction advances new applied technology in aquaculture.

PubMed

Kagawa, Hirohiko; Tanaka, Hideki; Ohta, Hiromi; Unuma, Tatsuya; Nomura, Kazuharu

2005-04-01

The eel has long been esteemed as an important food fish in the world, especially in Japan, and has been used as an experimental fish for many fields of fish physiology. However, the decreases in eel resources have been a serious concern in recent years. The catches of glass eels as seedlings for aquaculture have shown a long-term decrease in both Europe and East Asia. To increase eel resources, the development of techniques for artificial induction of maturation and spawning and rearing their larvae have been eagerly desired. Recent progress of reproductive physiology of fish, especially mechanisms of oocyte maturation and ovulation in female and of spermatozoa maturation in male, facilitate to establish techniques for hormonal induction of maturation and spawning in sexually immature eels. With persistent effort to development of rearing techniques of larvae, we have first succeeded to produce glass eel. These applied techniques are may contribute to understand the basic reproductive physiology of the eel.
Solvent and extraction methods effects on the quality of eel (Anguilla bicolor) oil

NASA Astrophysics Data System (ADS)

Sasongko, H.; Efendi, N. R.; Budihardjo, A.; Farida, Y.; Amartiwi, T.; Rahmawati, A. A.; Wicaksono, A.; Sugiyarto

2017-01-01

Eel (Anguilla bicolor) is a general fish consumption in many countries, especially Japan, China, Germany, and France. Besides its vitamin rich, eel oil is also known to contain fatty acids that are necessary for pharmaceutical purposes and as food a supplement. This research was aimed to evaluate the quality of eel oil by different solvent and extraction methods. In this study, fresh eels were extracted using maceration and reflux methods.Chloroform was used as the solvent in the maceration while water used in the reflux method. The oil quality was examined based on the Official Methods of Analysis of the Association of Official Analytical Chemist (AOAC).The result showed that the yield of eel oil using maceration method was 5.44% ± 0.64 with a specific gravity of 0.915 g/mL, while reflux method obtained the yield of 5.33 % ± 0.84 and specific gravity of 0.8575 g/mL. The physicochemical parameters of oil quality used in this study were acid, peroxide, saponification, and iodine value. The maceration method obtained the acid value of 17.389 mgKOH/g, the peroxide value of 7.021meqO2/kg, the saponification value of 111.16mgKOH/g, and the iodine value of 65.14 WIJS. While the reflux method produced the acid value of 9.116 mgKOH/g, the peroxide value of 6.088 meqO2/kg, the saponification value of 70 mgKOH/g, and the iodine value of 87.74 WIJS.
Direct calorimetry of free-moving eels with manipulated thyroid status

NASA Astrophysics Data System (ADS)

van Ginneken, Vincent; Ballieux, Bart; Antonissen, Erik; van der Linden, Rob; Gluvers, Ab; van den Thillart, Guido

2007-02-01

In birds and mammals, the thyroid gland secretes the iodothyronine hormones of which tetraiodothyronine (T4) is less active than triiodothyronine (T3). The action of T3 and T4 is calorigenic and is involved in the control of metabolic rate. Across all vertebrates, thyroid hormones also play a major role in differentiation, development and growth. Although the fish thyroidal system has been researched extensively, its role in thermogenesis is unclear. In this study, we measured overall heat production to an accuracy of 0.1 mW by direct calorimetry in a free-moving European eel ( Anguilla anguilla L.) with different thyroid status. Hyperthyroidism was induced by injection of T3 and T4, and hypothyroidism was induced with phenylthiourea. The results show for the first time at the organismal level, using direct calorimetry, that neither overall heat production nor overall oxygen consumption in eels is affected by hyperthyroidism. Therefore, we conclude that the thermogenic metabolism-stimulating effect of thyroid hormones (TH) is not present with a cold-blooded fish species like the European eel. This supports the concept that TH does not stimulate thermogenesis in poikilothermic species.
Circadian rhythm phase shifts and endogenous free-running circadian period differ between African-Americans and European-Americans.

PubMed

Eastman, Charmane I; Suh, Christina; Tomaka, Victoria A; Crowley, Stephanie J

2015-02-11

Successful adaptation to modern civilization requires the internal circadian clock to make large phase shifts in response to circumstances (e.g., jet travel and shift work) that were not encountered during most of our evolution. We found that the magnitude and direction of the circadian clock's phase shift after the light/dark and sleep/wake/meal schedule was phase-advanced (made earlier) by 9 hours differed in European-Americans compared to African-Americans. European-Americans had larger phase shifts, but were more likely to phase-delay after the 9-hour advance (to phase shift in the wrong direction). The magnitude and direction of the phase shift was related to the free-running circadian period, and European-Americans had a longer circadian period than African-Americans. Circadian period was related to the percent Sub-Saharan African and European ancestry from DNA samples. We speculate that a short circadian period was advantageous during our evolution in Africa and lengthened with northern migrations out of Africa. The differences in circadian rhythms remaining today are relevant for understanding and treating the modern circadian-rhythm-based disorders which are due to a misalignment between the internal circadian rhythms and the times for sleep, work, school and meals.
Geographic and Oceanographic Information within Trace Metals in Moray Eel Otoliths

NASA Astrophysics Data System (ADS)

Savidge, W.; Windom, H.; Buck, C.

2016-02-01

Adult moray eels exhibit high site fidelity to particular reefs. We hypothesized that the trace metal composition of otoliths of eels could potentially provide insight into gradients in oceanographic processes on the South Atlantic Bight continental shelf where eels are resident on patchy hardbottom reefs throughout the entire region. Otoliths of moray eels collected from the mid-shelf of South Carolina were examined for their trace metal composition (Ba, Sr, Pb, Cu, Li, Mg, V, Mn, Zn). Samples were broadly lumped into four regions: "North," "North Cape Romain," "South Cape Romain," and "South". Trace metal composition within otoliths showed no latitudinal trends. However, factor analysis of the trace metals revealed that otoliths from the South Cape Romain region appeared as a compositionally distinct subgroup, based primarily on their Li and Mg content. Recent work on corals (Montagna et al. 2014) has shown the Li/Mg ratio within coral skeletons is sensitive to calcification temperature and can be used as a paleothermometer. If analogous processes influence Li/Mg ratios within otoliths, the data suggest that the bottom water at the South Cape Romain site is colder than other locations along the South Carolina shelf, perhaps as a result of locally enhanced upwelling. Additional samples from NC, SC, GA, and FL are being examined to see if other sites within the South Atlantic Bight show similar patterns. Montagna, P., McCulloch, M., Douville, E., et al. 2014. Li/Mg systematics in scleratinian corals: Calibration of the thermometer. Geochim Cosmochim Acta 132: 288-310.
Gas phase absorption studies of photoactive yellow protein chromophore derivatives.

PubMed

Rocha-Rinza, Toms; Christiansen, Ove; Rajput, Jyoti; Gopalan, Aravind; Rahbek, Dennis B; Andersen, Lars H; Bochenkova, Anastasia V; Granovsky, Alexander A; Bravaya, Ksenia B; Nemukhin, Alexander V; Christiansen, Kasper Lincke; Nielsen, Mogens Brøndsted

2009-08-27

Photoabsorption spectra of deprotonated trans p-coumaric acid and two of its methyl substituted derivatives have been studied in gas phase both experimentally and theoretically. We have focused on the spectroscopic effect of the location of the two possible deprotonation sites on the trans p-coumaric acid which originate to either a phenoxide or a carboxylate. Surprisingly, the three chromophores were found to have the same absorption maximum at 430 nm, in spite of having different deprotonation positions. However, the absorption of the chromophore in polar solution is substantially different for the distinct deprotonation locations. We also report on the time scales and pathways of relaxation after photoexcitation for the three photoactive yellow protein chromophore derivatives. As a result of these experiments, we could detect the phenoxide isomer within the deprotonated trans p-coumaric acid in gas phase; however, the occurrence of the carboxylate is uncertain. Several computational methods were used simultaneously to provide insights and assistance in the interpretation of our experimental results. The calculated excitation energies S(0)-S(1) are in good agreement with experiment for those systems having a negative charge on a phenoxide moiety. Although our augmented multiconfigurational quasidegenerate perturbation theory calculations agree with experiment in the description of the absorption spectrum of anions with a carboxylate functional group, there are some puzzling disagreements between experiment and some calculational methods in the description of these systems.
Present status of yellow fever: memorandum from a PAHO meeting.

PubMed

1986-01-01

An international seminar on the treatment and laboratory diagnosis of yellow fever, sponsored by the Pan American Health Organization (PAHO) and held in 1984, differed from previous meetings on yellow fever because of its emphasis on the care and management of patients and because the participants included specialists from several branches of medicine, such as hepatology, haematology, cardiology, infectious diseases, pathology and nephrology. The meeting reviewed the current status of yellow fever and problems associated with case-finding and notification; features of yellow fever in individual countries of Latin America; health services and facilities for medical care as they relate to diagnosis and management of cases; prevention strategies for and current status of immunization programmes; clinical and pathological aspects of yellow fever in humans; pathogenesis and pathophysiology of yellow fever in experimental animal models; clinical and specific laboratory diagnosis; treatment of the disease and of complications in the functioning of individual organ systems; prognosis and prognostic indicators; and directions for future clinical and experimental research on pathophysiology and treatment.
Unraveling the etiology of North American grapevine yellows (NAGY): multilocus genotyping and structural analysis of secY proteins distinguish NAGYIII phytoplasma strains from strains causing X-disease

USDA-ARS?s Scientific Manuscript database

North American grapevine yellows (NAGY) disease has sometimes been ascribed to infection of Vitis vinifera L. by X-disease phytoplasma, but the accuracy of this attribution has remained open to question. In the present study of NAGY etiology, the disease was discovered in Maryland, Pennsylvania, Oh...
Spawning Sites of the Japanese Eel in Relation to Oceanographic Structure and the West Mariana Ridge

PubMed Central

Aoyama, Jun; Watanabe, Shun; Miller, Michael J.; Mochioka, Noritaka; Otake, Tsuguo; Yoshinaga, Tatsuki; Tsukamoto, Katsumi

2014-01-01

The Japanese eel, Anguilla japonica, spawns within the North Equatorial Current that bifurcates into both northward and southward flows in its westward region, so its spawning location and larval transport dynamics seem important for understanding fluctuations in its recruitment to East Asia. Intensive research efforts determined that Japanese eels spawn along the western side of the West Mariana Ridge during new moon periods, where all oceanic life history stages have been collected, including eggs and spawning adults. However, how the eels decide where to form spawning aggregations is unknown because spawning appears to have occurred at various latitudes. A salinity front formed from tropical rainfall was hypothesized to determine the latitude of its spawning locations, but an exact spawning site was only found once by collecting eggs in May 2009. This study reports on the collections of Japanese eel eggs and preleptocephali during three new moon periods in June 2011 and May and June 2012 at locations indicating that the distribution of lower salinity surface water or salinity fronts influence the latitude of spawning sites along the ridge. A distinct salinity front may concentrate spawning south of the front on the western side of the seamount ridge. It was also suggested that eels may spawn at various latitudes within low-salinity water when the salinity fronts appeared unclear. Eel eggs were distributed within the 150–180 m layer near the top of the thermocline, indicating shallow spawning depths. Using these landmarks for latitude (salinity front), longitude (seamount ridge), and depth (top of the thermocline) to guide the formation of spawning aggregations could facilitate finding mates and help synchronize their spawning. PMID:24551155
Spawning sites of the Japanese eel in relation to oceanographic structure and the West Mariana Ridge.

PubMed

Aoyama, Jun; Watanabe, Shun; Miller, Michael J; Mochioka, Noritaka; Otake, Tsuguo; Yoshinaga, Tatsuki; Tsukamoto, Katsumi

2014-01-01

The Japanese eel, Anguilla japonica, spawns within the North Equatorial Current that bifurcates into both northward and southward flows in its westward region, so its spawning location and larval transport dynamics seem important for understanding fluctuations in its recruitment to East Asia. Intensive research efforts determined that Japanese eels spawn along the western side of the West Mariana Ridge during new moon periods, where all oceanic life history stages have been collected, including eggs and spawning adults. However, how the eels decide where to form spawning aggregations is unknown because spawning appears to have occurred at various latitudes. A salinity front formed from tropical rainfall was hypothesized to determine the latitude of its spawning locations, but an exact spawning site was only found once by collecting eggs in May 2009. This study reports on the collections of Japanese eel eggs and preleptocephali during three new moon periods in June 2011 and May and June 2012 at locations indicating that the distribution of lower salinity surface water or salinity fronts influence the latitude of spawning sites along the ridge. A distinct salinity front may concentrate spawning south of the front on the western side of the seamount ridge. It was also suggested that eels may spawn at various latitudes within low-salinity water when the salinity fronts appeared unclear. Eel eggs were distributed within the 150-180 m layer near the top of the thermocline, indicating shallow spawning depths. Using these landmarks for latitude (salinity front), longitude (seamount ridge), and depth (top of the thermocline) to guide the formation of spawning aggregations could facilitate finding mates and help synchronize their spawning.
EELS Analysis of Nylon 6 Nanofibers Reinforced with Nitroxide-Functionalized Graphene Oxide.

PubMed

Leyva-Porras, César; Ornelas-Gutiérrez, C; Miki-Yoshida, M; Avila-Vega, Yazmín I; Macossay, Javier; Bonilla-Cruz, José

2014-01-01

A detailed analysis by transmission electron microscopy (TEM) and electron energy loss spectroscopy (EELS) of nitroxide-functionalized graphene oxide layers (GOFT) dispersed in Nylon 6 nanofibers is reported herein. The functionalization and exfoliation process of graphite oxide to GOFT was confirmed by TEM using electron diffraction patterns (EDP), wherein 1 to 4 graphene layers of GOFT were observed. The distribution and alignment of GOFT layers within a sample of Nylon 6 nanofiber reveals that GOFT platelets are mainly within the fiber, but some were partially protruding from it. Furthermore, Nylon 6 nanofibers exhibit an average diameter of 225 nm with several microns in length. GOFT platelets embedded into the fiber, the pristine fiber, and amorphous carbon were analyzed by EELS where each spectra [corresponding to the carbon edge (C-K)] exhibited changes in the fine structure, allowing a clear distinction between: i) GOFT single-layers, ii) Nylon-6 nanofibers, and iii) the carbon substrate. EELS analysis is presented here for the first time as a powerful tool to identify functionalized graphene single-layers (< 4 layers of GOFT) into a Nylon 6 nanofiber composite.
Accumulation, elimination and neuro-oxidative damage under lanthanum exposure in glass eels (Anguilla anguilla).

PubMed

Figueiredo, Cátia; Grilo, Tiago F; Lopes, Clara; Brito, Pedro; Diniz, Mário; Caetano, Miguel; Rosa, Rui; Raimundo, Joana

2018-05-07

Rare earth elements (REEs) comprise elements from lanthanum to lutetium that together with yttrium and scandium are emergent contaminants of critical importance for numerous groundbreaking environmental technologies. Transfer to aquatic ecosystems is expected to increase, however, little information is known about their potential impacts in marine biota. Considering the endangered conservation status of the European eel (Anguilla anguilla) and the vulnerability of early fish life stages to contaminants, we exposed glass eels, through water, to an environmentally relevant concentration (120 ng.L - 1 ) of lanthanum (La) for 7 days (plus 7 days of depuration). The aim was to study the accumulation and elimination of La in eel's body and subsequent quantification of acetylcholinesterase (AchE), lipid peroxidation and antioxidant enzymatic machinery. Accumulation peaked after 72 h-exposure to La, decreasing afterwards, even in continuous exposure. Accumulation was higher in the viscera, followed by the skinless body and ultimately in the head, possibly as a protective mechanism to cope with La neurotoxicity. A significant increase in AChE activity was observed in La-exposed glass eels, suggesting that La 3+ may inhibit the binding of acetylcholine. A depression in lipid peroxidation was registered under La exposure, possibly indicating that La 3+ may play physiological activities and functions as a free radical scavenger. Catalase activity was significantly inhibited in La-exposed glass eels after 72 h, indicating that the availability of La may induce physiological impairment. The quantification of Glutathione S-Transferase activity revealed no differences between control and La-exposed organisms. Further investigation is needed towards understanding the biological effects of REEs. Copyright © 2018 Elsevier Ltd. All rights reserved.
Yellow Fever

MedlinePlus

... Testing Vaccine Information Testing for Vaccine Adverse Events Yellow fever Vaccine Continuing Education Course Yellow Fever Home Prevention Vaccine Vaccine Recommendations Reactions to Yellow Fever Vacine Yellow Fever Vaccine, Pregnancy, & ... Transmission Symptoms, Diagnosis, & Treatment Maps Africa ...
Is there a Stobbs factor in atomic-resolution STEM-EELS mapping?

PubMed

Xin, Huolin L; Dwyer, Christian; Muller, David A

2014-04-01

Recent work has convincingly argued that the Stobbs factor-disagreement in contrast between simulated and experimental atomic-resolution images-in ADF-STEM imaging can be accounted for by including the incoherent source size in simulation. However, less progress has been made for atomic-resolution STEM-EELS mapping. Here we have performed carefully calibrated EELS mapping experiments of a [101] DyScO3 single-crystal specimen, allowing atomic-resolution EELS signals to be extracted on an absolute scale for a large range of thicknesses. By simultaneously recording the elastic signal, also on an absolute scale, and using it to characterize the source size, sample thickness and inelastic mean free path, we eliminate all free parameters in the simulation of the core-loss signals. Coupled with double channeling simulations that incorporate both core-loss inelastic scattering and dynamical elastic and thermal diffuse scattering, the present work enables a close scrutiny of the scattering physics in the inelastic channel. We found that by taking into account the effective source distribution determined from the ADF images, both the absolute signal and the contrast in atomic-resolution Dy-M5 maps can be closely reproduced by the double-channeling simulations. At lower energy losses, discrepancies are present in the Sc-L2,3 and Dy-N4,5 maps due to the energy-dependent spatial distribution of the background spectrum, core-hole effects, and omitted complexities in the final states. This work has demonstrated the possibility of using quantitative STEM-EELS for element-specific column-by-column atom counting at higher energy losses and for atomic-like final states, and has elucidated several possible improvements for future theoretical work. Copyright © 2014 Elsevier B.V. All rights reserved.

A new deepwater species of the snake eel genus Ophichthus (Anguilliformes: Ophichthidae) from North Carolina

USGS Publications Warehouse

McCosker, J.E.; Ross, Steve W.

2007-01-01

Ophichthus brevirostris, a new species of snake eel, subfamily Ophichthinae, is described from a specimen trawled in deep water (406-440 m) off North Carolina. It is distinguished from its congeners by the following combination of characters: large orbit; filamentous pectoral fin; two preopercular pores; short snout; minute dentition; body coloration; and vertebral formula (13/60/153). It is most similar to Ophichthus arneutes from the Gala??pagos, O. genie from New Caledonia and Maldives, and two undescribed congeners from Seychelles and Tonga. It differs from them in its dorsalfin origin, dentition, and vertebral numbers. Its characteristics do not agree with that of any of the 15 known leptocephali of western Atlantic relatives. ?? 2007 by the American Society of Ichthyologists and Herpetologists.
[Control discourses and power relations of yellow fever: Philadelphia in 1793].

PubMed

Kim, Seohyung

2014-12-01

1793 Yellow fever in Philadelphia was the most severe epidemics in the late 18th century in the United States. More than 10% of the population in the city died and many people fled to other cities. The cause of yellow fever in the United States had close relationship with slaves and sugar in Philadelphia. Sugarcane plantation had needed many labors to produce sugar and lots of Africans had to move to America as slaves. In this process, Aëdes aegypti, the vector of yellow fever had migrated to America and the circumstances of ships or cities provided appropriate conditions for its breeding. In this period, the cause of yellow fever could not be established exactly, so suggestions of doctors became entangled in political and intellectual discourses in American society. There was a critical conflict between Jeffersonian Republicanism and Federalism about the origin and treatment of yellow fever. Benjamin Rush, a Jeffersonian Republican, suggested urban sanitation reform and bloodletting. He believed the infectious disease happened because of unsanitary city condition, so he thought the United States could be a healthy nation by improvement of the public health and sanitation. He would like to cope with national crisis and develop American society on the basis of republicanism. While Rush suggested the improvement of public health and sanitation, the city government of Philadelphia suggested isolation of yellow fever patients and quarantine. City government isolated the patients from healthy people and it reconstructed space of hospital. Also, it built orphanages to take care of children who lost their parents during the epidemic and implemented power to control people put in the state of exception. Of course, city government tried to protect the city and nation by quarantine of every ship to Philadelphia. Control policies of yellow fever in 1793 showed different conflicts and interactions. Through the yellow fever, Jeffersonian Republicanism and Federalism had
Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil.

PubMed

Fischer, Carlo; Torres, Maria C; Patel, Pranav; Moreira-Soto, Andres; Gould, Ernest A; Charrel, Rémi N; de Lamballerie, Xavier; Nogueira, Rita Maria Ribeiro; Sequeira, Patricia C; Rodrigues, Cintia D S; Kümmerer, Beate M; Drosten, Christian; Landt, Olfert; Bispo de Filippis, Ana Maria; Drexler, Jan Felix

2017-11-01

The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.
Gnathostoma infection in fish caught for local consumption in Nakhon Nayok Province, Thailand. II. Deasonal variation in swamp eels.

PubMed

Rojekittikhun, Wichit; Chaiyasith, Tossapon; Butraporn, Piyarat

2004-12-01

From August 2000 to August 2001, 1844 swamp eels (Monopterus albus) were purchased from several local markets in Nakhon Nayok Province, Thailand, and examined for the presence of Gnathostoma advanced third-stage larvae. The overall prevalence was 30.1% and the mean number of larvae/eel (infection intensity) was 10.0. The highest infection rate (44.1%) was found in August 2000 and the lowest (10.7%) in March 2001. The greatest mean number of larvae/eel (75.1) was found in August 2000, whereas the fewest (2.3) was in July 2001. It is suggested that the prevalence and intensity of infection decreased within two months after the end of the rainy season and started to rise again about two months after the next rainy season began. A total of 5,532 Gnathostoma larvae were recovered from 555 infected eels, with a maximum number of 698 larvae/eel. The highest rates of Gnathostoma infection according to eel body length and weight were 87.5% in the group 91-100 cm, and 100% in groups of 901-1100 g, respectively. There were significant correlations between eel body lengths and infection rates, body lengths and infection intensities; eel body weights were also significantly correlated with infection rates and infection intensities. It was noted that the longer/ heavier the eels were, the higher would be the infection rates and the greater the infection intensities. Tissue distributions of Gnathostoma larvae in the livers and muscles of swamp eels were as follows: 43.0% of the total number of larvae were found in the muscles and 57.0% were in the liver; 29.7, 51.7, and 18.6% were in the anterior, middle, and posterior parts, respectively; 35.1% were in the dorsal part, while 64.9% were in the ventral part; 9.0, 18.7, 7.4, 20.6, 33.1, and 11.2% were in the anterodorsal, mediodorsal, posterodorsal, anteroventral, medioventral and posteroventral parts, respectively. Of the 5,532 Gnathostoma larvae examined, 1101 (19.9%) were found to possess morphological variants or abnormal
Silicone passive equilibrium samplers as 'chemometers' in eels and sediments of a Swedish lake.

PubMed

Jahnke, Annika; Mayer, Philipp; McLachlan, Michael S; Wickström, Håkan; Gilbert, Dorothea; MacLeod, Matthew

2014-03-01

Passive equilibrium samplers deployed in two or more media of a system and allowed to come to equilibrium can be viewed as 'chemometers' that reflect the difference in chemical activities of contaminants between the media. We applied silicone-based equilibrium samplers to measure relative chemical activities of seven 'indicator' polychlorinated biphenyls (PCBs) and hexachlorobenzene in eels and sediments from a Swedish lake. Chemical concentrations in eels and sediments were also measured using exhaustive extraction methods. Lipid-normalized concentrations in eels were higher than organic carbon-normalized concentrations in sediments, with biota-sediment accumulation factors (BSAFs) of five PCBs ranging from 2.7 to 12.7. In contrast, chemical activities of the same pollutants inferred by passive sampling were 3.5 to 31.3 times lower in eels than in sediments. The apparent contradiction between BSAFs and activity ratios is consistent with the sorptive capacity of lipids exceeding that of sediment organic carbon from this ecosystem by up to 50-fold. Factors that may contribute to the elevated activity in sediments are discussed, including slower response of sediments than water to reduced emissions, sediment diagenesis and sorption to phytoplankton. The 'chemometer' approach has the potential to become a powerful tool to study the thermodynamic controls on persistent organic chemicals in the environment and should be extended to other environmental compartments.
EELS Analysis of Nylon 6 Nanofibers Reinforced with Nitroxide-Functionalized Graphene Oxide

PubMed Central

Leyva-Porras, César; Ornelas-Gutiérrez, C.; Miki-Yoshida, M.; Avila-Vega, Yazmín I.; Macossay, Javier; Bonilla-Cruz, José

2014-01-01

A detailed analysis by transmission electron microscopy (TEM) and electron energy loss spectroscopy (EELS) of nitroxide-functionalized graphene oxide layers (GOFT) dispersed in Nylon 6 nanofibers is reported herein. The functionalization and exfoliation process of graphite oxide to GOFT was confirmed by TEM using electron diffraction patterns (EDP), wherein 1 to 4 graphene layers of GOFT were observed. The distribution and alignment of GOFT layers within a sample of Nylon 6 nanofiber reveals that GOFT platelets are mainly within the fiber, but some were partially protruding from it. Furthermore, Nylon 6 nanofibers exhibit an average diameter of 225 nm with several microns in length. GOFT platelets embedded into the fiber, the pristine fiber, and amorphous carbon were analyzed by EELS where each spectra [corresponding to the carbon edge (C-K)] exhibited changes in the fine structure, allowing a clear distinction between: i) GOFT single-layers, ii) Nylon-6 nanofibers, and iii) the carbon substrate. EELS analysis is presented here for the first time as a powerful tool to identify functionalized graphene single-layers (< 4 layers of GOFT) into a Nylon 6 nanofiber composite. PMID:24634536
Larval Gnathostoma spinigerum Detected in Asian Swamp Eels, Monopterus albus, Purchased from a Local Market in Yangon, Myanmar.

PubMed

Chai, Jong-Yil; Sohn, Woon-Mok; Na, Byoung-Kuk; Park, Jong-Bok; Jeoung, Hoo-Gn; Hoang, Eui-Hyug; Htoon, Thi Thi; Tin, Htay Htay

2015-10-01

The present study was performed to determine the infection status of swamp eels with Gnathostoma sp. larvae in Myanmar. We purchased total 37 Asian swamp eels, Monopterus albus, from a local market in Yangon in June and December 2013 and 2014. All collected eels were transferred with ice to our laboratory and each of them was examined by the artificial digestion technique. A total of 401 larval gnathostomes (1-96 larvae/eel) were detected in 33 (89.2%) swamp eels. Most of the larvae (n=383; 95.5%) were found in the muscle. The remaining 18 larvae were detected in the viscera. The advanced third-stage larvae (AdL3) were 2.3-4.4 mm long and 0.25-0.425 mm wide. The characteristic head bulb (0.093 × 0.221 mm in average size) with 4 rows of hooklets, muscular long esophagus (1.025 mm), and 2 pairs of cervical sacs (0.574 mm) were observed by light microscopy. The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. As scanning electron microscopic findings, the characteristic 4-5 rows of hooklets on the head bulb, a cervical papilla, tegumental spines regularly arranged in the transverse striations, and an anus were well observed. Based on these morphological characters, they were identified as the AdL3 of Gnathostoma spinigerum. By the present study, it has been confirmed for the first time that Asian swamp eels, M. albus, from Yangon, Myanmar are heavily infected with G. spinigerum larvae.
Quantifying the yellow signal driver behavior based on naturalistic data from digital enforcement cameras.

PubMed

Bar-Gera, H; Musicant, O; Schechtman, E; Ze'evi, T

2016-11-01

The yellow signal driver behavior, reflecting the dilemma zone behavior, is analyzed using naturalistic data from digital enforcement cameras. The key variable in the analysis is the entrance time after the yellow onset, and its distribution. This distribution can assist in determining two critical outcomes: the safety outcome related to red-light-running angle accidents, and the efficiency outcome. The connection to other approaches for evaluating the yellow signal driver behavior is also discussed. The dataset was obtained from 37 digital enforcement cameras at non-urban signalized intersections in Israel, over a period of nearly two years. The data contain more than 200 million vehicle entrances, of which 2.3% (∼5million vehicles) entered the intersection during the yellow phase. In all non-urban signalized intersections in Israel the green phase ends with 3s of flashing green, followed by 3s of yellow. In most non-urban signalized roads in Israel the posted speed limit is 90km/h. Our analysis focuses on crossings during the yellow phase and the first 1.5s of the red phase. The analysis method consists of two stages. In the first stage we tested whether the frequency of crossings is constant at the beginning of the yellow phase. We found that the pattern was stable (i.e., the frequencies were constant) at 18 intersections, nearly stable at 13 intersections and unstable at 6 intersections. In addition to the 6 intersections with unstable patterns, two other outlying intersections were excluded from subsequent analysis. Logistic regression models were fitted for each of the remaining 29 intersection. We examined both standard (exponential) logistic regression and four parameters logistic regression. The results show a clear advantage for the former. The estimated parameters show that the time when the frequency of crossing reduces to half ranges from1.7 to 2.3s after yellow onset. The duration of the reduction of the relative frequency from 0.9 to 0.1 ranged
Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification.

PubMed Central

Coleman, S S; Melanson, D M; Biosca, E G; Oliver, J D

1996-01-01

DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellobiose agar) selective for this pathogen were detectable in oysters harvested in January and March, containing no culturable cells (< 67 CFU/g), as well as in oysters harvested in May and June, containing culturable cells. It was possible to complete DNA extraction, PCR, and gel electrophoresis within 10 h by using the protocol described for oysters. V. vulnificus biotype 2 cells were also detected in eel tissues that had been infected with this strain and subsequently preserved in formalin. The protocol used for detection of V. vulnificus cells in eels required less than 5 h to complete. Optimum MgCl2 concentrations for the PCR of V. vulnificus from oysters and eels were different, although the same primer pair was used for both. This is the first report on the detection of cells of V. vulnificus naturally present in shellfish and represents a potentially powerful method for monitoring this important human and eel pathogen. PMID:8919800
Can lagrangian models reproduce the migration time of European eel obtained from otolith analysis?

NASA Astrophysics Data System (ADS)

Rodríguez-Díaz, L.; Gómez-Gesteira, M.

2017-12-01

European eel can be found at the Bay of Biscay after a long migration across the Atlantic. The duration of migration, which takes place at larval stage, is of primary importance to understand eel ecology and, hence, its survival. This duration is still a controversial matter since it can range from 7 months to > 4 years depending on the method to estimate duration. The minimum migration duration estimated from our lagrangian model is similar to the duration obtained from the microstructure of eel otoliths, which is typically on the order of 7-9 months. The lagrangian model showed to be sensitive to different conditions like spatial and time resolution, release depth, release area and initial distribution. In general, migration showed to be faster when decreasing the depth and increasing the resolution of the model. In average, the fastest migration was obtained when only advective horizontal movement was considered. However, faster migration was even obtained in some cases when locally oriented random migration was taken into account.
Genetic diversity of infective larvae of Gnathostoma spinigerum (Nematoda: Gnathostomatidae) in freshwater swamp eels from Thailand.

PubMed

Eamsobhana, P; Wanachiwanawin, D; Roongruangchai, K; Song, S L; Yong, H S

2017-11-01

Human gnathostomiasis is a food-borne zoonosis caused by a tissue nematode of the genus Gnathostoma. The disease is highly endemic in Asia, including Thailand. The freshwater swamp eel (Monopterus albus), the second intermediate host of the gnathostome nematode, has an important role in transmitting the infection in Thailand. Surveys on the infective larvae of Gnathostoma spinigerum based on morphological features in freshwater swamp eels have been performed continuously and reported in Thailand. However, there is still limited molecular data on intra-species variations of the parasite. In this study, a total of 19 third-stage larvae of morphologically identified G. spinigerum were collected from 437 liver samples of freshwater swamp eels purchased from a large wholesale market in Bangkok, Thailand. Molecular characterization based on mitochondrial cytochrome c oxidase subunit I (COI) sequences was performed to elucidate their genetic variations and phylogenetic relationship. Among the 19 infective larvae recovered from these eels, 16 were sequenced successfully. Phylogenetic analyses inferred from the partial COI gene showed the presence of three distinct COI haplotypes. Our findings confirm the presence of G. spinigerum as the main species in Thailand.
Quality attributes of farmed eel (Anguilla anguilla) stored under air, vacuum and modified atmosphere packaging at 0 degrees C.

PubMed

Arkoudelos, John; Stamatis, Nikolaos; Samaras, Fotis

2007-01-01

The shelf life of fresh eel in various packaging conditions of atmospheric air, vacuum and modified atmosphere packaging (MAP) (40% CO(2), 30% N(2) and 30% O(2)) at 0 degrees C was investigated. All raw eel samples received acceptable sensory scores during the first 11+/-1 days of storage in atmospheric air, 11+/-1 days of storage in vacuum and finally 18+/-1 days of storage in MAP conditions. Using the microbial quality indicators the shelf life of eel packed in air, vacuum and MAP was estimated to be more than 18, 28 and 34 days, respectively. The main spoilage microorganisms under MAP conditions were lactic acid producing bacteria followed by Shewanella spp., pseudomonads, Enterobacteriaceae and yeasts. Chemical data revealed that pH, ammonia, glucose and lactate examinations might not be useful for monitoring eel quality differences.
[Simultaneous determination of nine estrogens in eel by ultrafast liquid chromatography-tandem mass spectrometry with isotope dilution technique and solid-phase extraction].

PubMed

Chen, Xiaohong; Yao, Shanshan; Li, Xiaoping; Zhao, Yonggang; Jin, Micong

2012-11-01

Developing a rapid and sensitive analytical method based on ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) with solid-phase extraction (SPE) for the simultaneous determination of nine estrogens (dienestrol, diethylstilbestrol, estrone, hexestrol, 17-alpha-estradiol, 17-beta-estradiol, estriol, 17alpha-ethinylestradiol and estradiol valerate) in eel. After the sample was extracted by acetonitrile and cleaned by Waters Oasis HLB solid-phase extraction cartridge, the UFLC separation was performed on a Shim-pack XR-ODS II column (100 mm x 2.0 mm, 2.2 microm) with a linear gradient elution program of methanol solution containing 0.04% ammonia (v/v) and 0.04% ammonia aqueous solution (v/v) as the mobile phase. Electrospray ionization was applied and operated in the negative multiple reaction monitoring (MRM) mode. The quantitation was used by isotope internal standard dilution technique. The results showed that the limits of quantitation (LOQs, S/N(10) were in the range of 0.07-0.4 microg/kg, the calibration curves were in good linearities for the nine analytes in the range of 0.5-50.0 microg/L with the correlative coefficients (r2) more than 0.998, the recoveries were between 81.0% and 110.0% with the relative standard deviations (RSDs) of 1.92%-8.24%. Additional, the mass spectra characterization of the nine estrogens was discussed and the fragmentation pathways were speculated. The developed method is rapid, sensitive, specific and reproducible, and adapts not only to the simultaneous determination of the nine trace estrogens including the epimer of 17-alpha-estradiol and 17-beta-estradiol but also to the identified detection in other fish tissues.
EELS Valence Mapping in Electron Beam Sensitive FeFx/C Nanocomposites

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cosandey, F.; Al-Sharab, J.F.; Amatucci, Glenn G.

A new type of positive electrodes for Li-Ion batteries has been synthesized based on FeF{sub 2}/C and FeF3/C nanocomposites with particle size in the 8-12 nm range [1]. The measured high capacities rely on a complete reduction of Fe to its metallic state according to the following reaction: xLi{sup +}+xe{sup -} +Fe{sup x+}Fx = xLiF + Fe{sup 0}, where x=3 and x=2 for FeF3/C and FeF2/C respectively. This electrochemical reaction involves a change in valence state of Fe from 3+ or 2+ to 0 that can be determined uniquely by EELS from the peak energy of the L{sub 3} linemore » and from the L{sub 3}/L{sub 2} line intensity ratio. In this paper, we report EELS mapping results on the electrochemical conversion processes and in particular the mapping of the Fe valence state before and after discharge. This work was performed with a Hitachi HF2000 equipped with a Gatan PEELS and with a FEI CM200 FEG TEM equipped with a Gatan GIF. Both instruments were operated in STEM mode at 200kV with an EELS collection half angle of {beta}=5 mrad and spectrum imaging software.« less
Larval Gnathostoma spinigerum Detected in Asian Swamp Eels, Monopterus albus, Purchased from a Local Market in Yangon, Myanmar

PubMed Central

Chai, Jong-Yil; Sohn, Woon-Mok; Na, Byoung-Kuk; Park, Jong-Bok; Jeoung, Hoo-Gn; Hoang, Eui-Hyug; Htoon, Thi Thi; Tin, Htay Htay

2015-01-01

The present study was performed to determine the infection status of swamp eels with Gnathostoma sp. larvae in Myanmar. We purchased total 37 Asian swamp eels, Monopterus albus, from a local market in Yangon in June and December 2013 and 2014. All collected eels were transferred with ice to our laboratory and each of them was examined by the artificial digestion technique. A total of 401 larval gnathostomes (1-96 larvae/eel) were detected in 33 (89.2%) swamp eels. Most of the larvae (n=383; 95.5%) were found in the muscle. The remaining 18 larvae were detected in the viscera. The advanced third-stage larvae (AdL3) were 2.3-4.4 mm long and 0.25-0.425 mm wide. The characteristic head bulb (0.093 × 0.221 mm in average size) with 4 rows of hooklets, muscular long esophagus (1.025 mm), and 2 pairs of cervical sacs (0.574 mm) were observed by light microscopy. The average number of hooklets in the 1st, 2nd, 3rd, and 4th rows was 41, 45, 48, and 51, respectively. As scanning electron microscopic findings, the characteristic 4-5 rows of hooklets on the head bulb, a cervical papilla, tegumental spines regularly arranged in the transverse striations, and an anus were well observed. Based on these morphological characters, they were identified as the AdL3 of Gnathostoma spinigerum. By the present study, it has been confirmed for the first time that Asian swamp eels, M. albus, from Yangon, Myanmar are heavily infected with G. spinigerum larvae. PMID:26537042
Effect of earthworm (Lumbricus rubellus) in feed formulation to improve fatty acids profile in eel (Anguilla bicolor) meat

NASA Astrophysics Data System (ADS)

Farah, K.; Gunawan, I. R.; Putra, G. B.; Agustono; Lokapirnasari, W. P.; Lamid, M.; Masithah, E. D.; Nurhajati, T.; Rozi

2018-04-01

Eel requires unsaturated fatty acids of linolenic acid for growth. Which can be supplied from earthworms. In this study, addition of earthworm in formulation feed aimsed to improve the fatty acid profile eel meat. This research used experimental method and randomized complete design method with five treatments. Each treatment was repeated four times. The use of earthworms in feeding treatment formulation was done for 21 days with different level i.e: 0 % (P0), 25 % (P1), 50 % (P2), 75 % (P3) and 100 % (P4). The result showed that the addition of eartworm significantly influenced the omega 3 contents (EPA & DHA) of eel meat.
Freeze concentration for enrichment of nutrients in yellow water from no-mix toilets.

PubMed

Gulyas, H; Bruhn, P; Furmanska, M; Hartrampf, K; Kot, K; Lüttenberg, B; Mahmood, Z; Stelmaszewska, K; Otterpohl, R

2004-01-01

Separately collected urine ("yellow water") can be utilized as fertilizer. In order to decrease storage volumes and energy consumption for yellow water transport to fields, enrichment of nutrients in yellow water has to be considered. Laboratory-scale batch freeze concentration of yellow water has been tested in ice-front freezing apparatus: a stirred vessel and a falling film freeze concentrator (coolant temperatures: -6 to -16 degrees C). With progressing enrichment of the liquid concentrate, the frozen ice was increasingly contaminated with yellow water constituents (ammonia, total nitrogen, total phosphorus, TOC, and salts determined as conductivity). The higher the initial salinity of the yellow water and the lower the mechanical agitation of the liquid phase contacting the growing ice front, the more the frozen ice was contaminated. The results indicate, that in ice-front freezing devices multistage processes are necessary, i.e. the melted ice phase has to be purified (and the concentrates must be further enriched) in a second or even in a third stage. Energy consumption of this process is very high. However, technical scale suspension freeze concentration is reasonable in centralized ecological sanitation schemes if the population exceeds 0.5 million and distance of yellow water transportation to fields is more than 80 km.
Progress and opportunities in EELS and EDS tomography.

PubMed

Collins, Sean M; Midgley, Paul A

2017-09-01

Electron tomography using energy loss and X-ray spectroscopy in the electron microscope continues to develop in rapidly evolving and diverse directions, enabling new insight into the three-dimensional chemistry and physics of nanoscale volumes. Progress has been made recently in improving reconstructions from EELS and EDS signals in electron tomography by applying compressed sensing methods, characterizing new detector technologies in detail, deriving improved models of signal generation, and exploring machine learning approaches to signal processing. These disparate threads can be brought together in a cohesive framework in terms of a model-based approach to analytical electron tomography. Models incorporate information on signal generation and detection as well as prior knowledge of structures in the spectrum image data. Many recent examples illustrate the flexibility of this approach and its feasibility for addressing challenges in non-linear or limited signals in EELS and EDS tomography. Further work in combining multiple imaging and spectroscopy modalities, developing synergistic data acquisition, processing, and reconstruction approaches, and improving the precision of quantitative spectroscopic tomography will expand the frontiers of spatial resolution, dose limits, and maximal information recovery. Copyright © 2017 Elsevier B.V. All rights reserved.
Identification of a testis-enriched heat shock protein and fourteen members of Hsp70 family in the swamp eel.

PubMed

He, Yan; Luo, Majing; Yi, Minhan; Sheng, Yue; Cheng, Yibin; Zhou, Rongjia; Cheng, Hanhua

2013-01-01

Gonad differentiation is one of the most important developmental events in vertebrates. Some heat shock proteins are associated with gonad development. Heat shock protein 70 (Hsp70) in the teleost fish and its roles in sex differentiation are poorly understood. We have identified a testis-enriched heat shock protein Hspa8b2 in the swamp eel using Western blot analysis and Mass Spectrometry (MS). Fourteen Hsp70 family genes were further identified in this species based on transcriptome information. The phylogenetic tree of Hsp70 family was constructed using the Maximum Likelihood method and their expression patterns in the swamp eel gonads were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). There are fourteen gene members in the Hsp70 family in the swamp eel genome. Hsp70 family, particularly Hspa8, has expanded in the species. One of the family members Hspa8b2 is predominantly expressed in testis of the swamp eel.
[Yellow fever].

PubMed

Sabbatani, Sergio; Fiorino, Sirio

2007-06-01

After the discovery of the New World, yellow fever proved to be an important risk factor of morbidity and mortality for Caribbean populations. In the following centuries epidemic risk, expanded by sea trade and travel, progressively reached the settlements in North America and Brazil as well as the Atlantic seaboard of tropical and equatorial Africa. In the eighteenth century and the first half of the nineteenth century epidemics of yellow fever were reported in some coastal towns in the Iberian peninsula, French coast, Great Britain and Italy, where, in 1804 at Leghorn, only one epidemic was documented. Prevention and control programs against yellow fever, developed at the beginning of the twentieth century in Cuba and in Panama, were a major breakthrough in understanding definitively its aetiology and pathogenesis. Subsequently, further advances in knowledge of yellow fever epidemiology were obtained when French scientists, working in West and Central Africa, showed that monkeys were major hosts of the yellow fever virus (the wild yellow fever virus), besides man. In addition, advances in research, contributing to the development of vaccines against the yellow fever virus in the first half of the nineteenth century, are reported in this paper.

Vg mRNA induction in an endangered fish species (Anguilla anguilla) from the Loire estuary (France).

PubMed

Blanchet-Letrouvé, Isabelle; Lafont, Anne-Gaëlle; Poirier, Laurence; Baloche, Sylvie; Zalouk-Vergnoux, Aurore; Dufour, Sylvie; Mouneyrac, Catherine

2013-11-01

Estuarine zones are extremely fragile due to increasing stress from anthropogenic activities. Among those, the Loire estuary (France) is potentially exposed to various contaminants including Endocrine Disruptors Compounds (EDCs) able to impact the reproduction physiology of fish. The European eel (Anguilla anguilla), endangered fish species, is apparently not relevant, in its yellow stage, to monitor the effects of endocrine disruption. Despite this weakly responsiveness, this study aimed to investigate whether European eel from the Loire estuary may still be the subject of estrogenic disruption quantifying the hepatic Vg gene expression according to gender and sexual stage. Vitellogenin (Vg) appears as a valuable biomarker of EDCs, as well as for exposure and effects. Quantitative real-time Reverse Transcription Polymerase Chain Reaction (q RT PCR) was used in this study to amplify responses of hepatic Vg transcripts. European eels were sampled in May 2009 (N=57) and November 2010 (during the downstream migration, N=10) in two sites of the Loire estuary with different ecological conditions and contamination pressures (upstream: Varades; downstream: Nantes). Reproductive (gender, sexual maturity stage) and biometric parameters of collected eels were determined. A laboratory exposure of silver male to steroid hormones (Testosterone (T), 11-KetoTestosterone (11-KT), Estradiol (E2)) was conducted in parallel to validate the q RT PCR approach on hepatic Vg mRNA. Results demonstrated the responsiveness of exposed silver male eels, since hepatic mRNA Vg induction was observed in E2 treated males compared to control specimens. In the field, results of female silver eels reflected large inter-individual differences in the activation of hepatic Vg at silvering. However, while only female silver eels should express hepatic Vg mRNA, quantifiable levels were also detected in a proportion of 38% of the other individuals sampled, normally not inclined to express it, those being
Lipid increases in European eel (Anguilla anguilla) in Scotland 1986-2008: an assessment of physical parameters and the influence of organic pollutants.

PubMed

Oliver, Ian W; Macgregor, Kenneth; Godfrey, Jason D; Harris, Lynsay; Duguid, Alistair

2015-05-01

Recruitment of the European eel (Anguilla anguilla) has fallen steadily in recent decades, with current levels understood to be at around 5% of those in the 1970s, and the species is now widely recognised as being endangered. Changes in ocean currents, climate shifts, habitat loss, overfishing, barriers to migration, increased predation, plastic litter and exposure to chemicals have all been postulated as potential causative factors. Several studies have shown a general decline in eel quality (lower lipid content and body condition) over time that may be linked to reduced reproductive success. In this study, data from an eel sampling campaign in 1987 are compared with recent data (2004-2008) for eels in Scotland to assess any temporal changes in eel quality indicators and also to assess any links between current levels of chemical exposure and eel quality. Mean lipid levels, as a percentage of wet muscle mass, were higher in 2004-2008 (37 ± 1.9% SE) than in 1986 (21 ± 0.9% SE). By contrast, mean body condition index (K) was slightly lower in the latter period. Considering the 2004-2008 samples, significant inter-site variation was observed for condition index K, while intra-site variation was observed for lipid content and physical parameters relative to age (i.e. mass/age, length/age and lipid/age ratios); however, the variations observed could not be linked to differences in chemical body burdens, indicating that no chemical impacts on the parameters assessed are discernible during the continental life stage of eels in Scotland.
Do trout swim better than eels? Challenges for estimating performance based on the wake of self-propelled bodies

NASA Astrophysics Data System (ADS)

Tytell, Eric D.

2007-11-01

Engineers and biologists have long desired to compare propulsive performance for fishes and underwater vehicles of different sizes, shapes, and modes of propulsion. Ideally, such a comparison would be made on the basis of either propulsive efficiency, total power output or both. However, estimating the efficiency and power output of self-propelled bodies, and particularly fishes, is methodologically challenging because it requires an estimate of thrust. For such systems traveling at a constant velocity, thrust and drag are equal, and can rarely be separated on the basis of flow measured in the wake. This problem is demonstrated using flow fields from swimming American eels, Anguilla rostrata, measured using particle image velocimetry (PIV) and high-speed video. Eels balance thrust and drag quite evenly, resulting in virtually no wake momentum in the swimming (axial) direction. On average, their wakes resemble those of self-propelled jet propulsors, which have been studied extensively. Theoretical studies of such wakes may provide methods for the estimation of thrust separately from drag. These flow fields are compared with those measured in the wakes of rainbow trout, Oncorhynchus mykiss, and bluegill sunfish, Lepomis macrochirus. In contrast to eels, these fishes produce wakes with axial momentum. Although the net momentum flux must be zero on average, it is neither spatially nor temporally homogeneous; the heterogeneity may provide an alternative route for estimating thrust. This review shows examples of wakes and velocity profiles from the three fishes, indicating challenges in estimating efficiency and power output and suggesting several routes for further experiments. Because these estimates will be complicated, a much simpler method for comparing performance is outlined, using as a point of comparison the power lost producing the wake. This wake power, a component of the efficiency and total power, can be estimated in a straightforward way from the flow
Do trout swim better than eels? Challenges for estimating performance based on the wake of self-propelled bodies

NASA Astrophysics Data System (ADS)

Tytell, Eric D.

Engineers and biologists have long desired to compare propulsive performance for fishes and underwater vehicles of different sizes, shapes, and modes of propulsion. Ideally, such a comparison would be made on the basis of either propulsive efficiency, total power output or both. However, estimating the efficiency and power output of self-propelled bodies, and particularly fishes, is methodologically challenging because it requires an estimate of thrust. For such systems traveling at a constant velocity, thrust and drag are equal, and can rarely be separated on the basis of flow measured in the wake. This problem is demonstrated using flow fields from swimming American eels, Anguilla rostrata, measured using particle image velocimetry (PIV) and high-speed video. Eels balance thrust and drag quite evenly, resulting in virtually no wake momentum in the swimming (axial) direction. On average, their wakes resemble those of self-propelled jet propulsors, which have been studied extensively. Theoretical studies of such wakes may provide methods for the estimation of thrust separately from drag. These flow fields are compared with those measured in the wakes of rainbow trout, Oncorhynchus mykiss, and bluegill sunfish, Lepomis macrochirus. In contrast to eels, these fishes produce wakes with axial momentum. Although the net momentum flux must be zero on average, it is neither spatially nor temporally homogeneous; the heterogeneity may provide an alternative route for estimating thrust. This review shows examples of wakes and velocity profiles from the three fishes, indicating challenges in estimating efficiency and power output and suggesting several routes for further experiments. Because these estimates will be complicated, a much simpler method for comparing performance is outlined, using as a point of comparison the power lost producing the wake. This wake power, a component of the efficiency and total power, can be estimated in a straightforward way from the flow
The use of the DR CALUX bioassay and indicator polychlorinated biphenyls for screening of elevated levels of dioxins and dioxin-like polychlorinated biphenyls in eel.

PubMed

Hoogenboom, Ron; Bovee, Toine; Traag, Win; Hoogerbrugge, Ronald; Baumann, Bert; Portier, Liza; van de Weg, Guido; de Vries, Jaap

2006-10-01

The DR CALUX bioassay is a very suitable screening method for dioxins and dioxin-like-PCBs in feed and food. This was, e. g. demonstrated in a survey in the Netherlands to control the dioxin levels in eel. The DR CALUX assay, but also indicator polychlorinated biphenyls (PCB) were evaluated as a screening method. Based on the limit for polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/F) [at that time 8 pg toxic equivalents (TEQ)/g eel], and the relation between PCDD/F and dioxin-like-PCB, a decision limit of 30 pg TEQ/g eel was used for screening of 153 field samples. Suspected samples (21) and part of the higher contaminated negative samples (35) were analyzed by GC/MS for dioxins, non-ortho, mono-ortho and indicator PCB, revealing 13 samples exceeding the action limit of 30 pg TEQ/g eel. Only one sample slightly exceeded the dioxin level of 8 pg TEQ/g eel. The relatively low sensitivity for mono-ortho PCB was overcome by the use of reference samples, as shown by the correlation of 0.93 between GC/MS and CALUX determined total TEQ levels. The present data show that the DR CALUX assay can be used for screening of total TEQ levels in eel. The use for dioxins only requires a safe, and therefore relatively low, decision limit. The indicator PCB also showed a good correlation with total TEQ levels, mainly due to the large contribution of the mono-ortho PCB at higher concentrations. The relation with dioxins was very poor and as such indicator PCB seem less suitable than the DR CALUX assay for screening for dioxins only. The present study clearly shows that part of the wild eel samples contains high total TEQ levels and will exceed the future European Union limit of 12 pg TEQ/g eel for dioxins and dioxin-like PCB. Especially at high TEQ levels, dioxin-like PCB contribute most to the total TEQ. In practice, wild eel presents only a minor part of the eel consumed.
Teaching Population Growth Using Cultures of Vinegar Eels, "Turbatrix aceti" (Nematoda)

ERIC Educational Resources Information Center

Wallace, Robert L.

2005-01-01

A simple laboratory exercise is presented that follows the population growth of the common vinegar eel, "Turbatrix aceti" (Nematoda), in a microcosm using a simple culture medium. It lends itself to an exercise in a single semester course. (Contains 4 figures.)
The European Eel NCCβ Gene Encodes a Thiazide-resistant Na-Cl Cotransporter*

PubMed Central

Moreno, Erika; Plata, Consuelo; Rodríguez-Gama, Alejandro; Argaiz, Eduardo R.; Vázquez, Norma; Leyva-Ríos, Karla; Islas, León; Cutler, Christopher; Pacheco-Alvarez, Diana; Mercado, Adriana; Cariño-Cortés, Raquel; Castañeda-Bueno, María; Gamba, Gerardo

2016-01-01

The thiazide-sensitive Na-Cl cotransporter (NCC) is the major pathway for salt reabsorption in the mammalian distal convoluted tubule. NCC plays a key role in the regulation of blood pressure. Its inhibition with thiazides constitutes the primary baseline therapy for arterial hypertension. However, the thiazide-binding site in NCC is unknown. Mammals have only one gene encoding for NCC. The eel, however, contains a duplicate gene. NCCα is an ortholog of mammalian NCC and is expressed in the kidney. NCCβ is present in the apical membrane of the rectum. Here we cloned and functionally characterized NCCβ from the European eel. The cRNA encodes a 1043-amino acid membrane protein that, when expressed in Xenopus oocytes, functions as an Na-Cl cotransporter with two major characteristics, making it different from other known NCCs. First, eel NCCβ is resistant to thiazides. Single-point mutagenesis supports that the absence of thiazide inhibition is, at least in part, due to the substitution of a conserved serine for a cysteine at position 379. Second, NCCβ is not activated by low-chloride hypotonic stress, although the unique Ste20-related proline alanine-rich kinase (SPAK) binding site in the amino-terminal domain is conserved. Thus, NCCβ exhibits significant functional differences from NCCs that could be helpful in defining several aspects of the structure-function relationship of this important cotransporter. PMID:27587391
Anthropogenic spreading of anguillid herpesvirus 1 by stocking of infected farmed European eels, Anguilla anguilla (L.), in the Schlei fjord in northern Germany.

PubMed

Kullmann, B; Adamek, M; Steinhagen, D; Thiel, R

2017-11-01

The Schlei fjord in northern Germany is the recipient water of a comprehensive eel, Anguilla anguilla (L.), stocking programme. Since 2015, stocked eels become alizarin red S marked, but to date no control mechanism is implemented in this stock enhancement measure to prevent anthropogenic spreading of diseases. Consequentially, it was possible that farmed stocking cohorts of 2015 and 2016 (in total ca. 1040 kg) were subsequently tested positive for anguillid herpesvirus 1 (AngHV 1). For this study, 100 eels [total length (TL) 24.3-72.9 cm, age ca. 1-6 years] were caught in 2016 and investigated with regard to AngHV 1 infection, parasite load (Anguillicoloides crassus) and body conditions. 68% of the eels were found to be virus positive while larger specimens were more often infected. In addition, a fitted generalized linear model (area under the curve = 0.741) demonstrated that an increase in individual TL is accompanied with an increased risk of clinically relevant virus loads. Anguillicoloides crassus turned out to be an important stressor for eels, because parasite and virus load revealed a significant positive correlation. The results of this study evidently show the urgent need of a disease containment strategy for eel stocking programmes. © 2017 John Wiley & Sons Ltd.
Effects of environmental cocaine concentrations on the skeletal muscle of the European eel (Anguilla anguilla).

PubMed

Capaldo, Anna; Gay, Flaminia; Lepretti, Marilena; Paolella, Gaetana; Martucciello, Stefania; Lionetti, Lillà; Caputo, Ivana; Laforgia, Vincenza

2018-06-04

The presence of illicit drugs in the aquatic environment represents a new potential risk for aquatic organisms, due to their constant exposure to substances with strong pharmacological activity. Currently, little is known about the ecological effects of illicit drugs. The aim of this study was to evaluate the influence of environmental concentrations of cocaine, an illicit drug widespread in surface waters, on the skeletal muscle of the European eel (Anguilla anguilla). The skeletal muscle of silver eels exposed to 20 ng L -1 of cocaine for 50 days were compared to control, vehicle control and two post-exposure recovery groups (3 and 10 days after interruption of cocaine). The eels general health, the morphology of the skeletal muscle and several parameters indicative of the skeletal muscle physiology were evaluated, namely the muscle whole protein profile, marker of the expression levels of the main muscle proteins; cytochrome oxidase activity, markers of oxidative metabolism; caspase-3, marker of apoptosis activation; serum levels of creatine kinase, lactate dehydrogenase and aspartate aminotransferase, markers of skeletal muscle damages. Cocaine-exposed eels appeared hyperactive but they showed the same general health status as the other groups. In contrast, their skeletal muscle showed evidence of serious injury, including muscle breakdown and swelling, similar to that typical of rhabdomyolysis. These changes were still present 10 days after the interruption of cocaine exposure. In fact, with the exception of the expression levels of the main muscle proteins, which remained unchanged, all the other parameters examined showed alterations that persisted for at least 10 days after the interruption of cocaine exposure. This study shows that even low environmental concentrations of cocaine cause severe damage to the morphology and physiology of the skeletal muscle of the silver eel, confirming the harmful impact of cocaine in the environment that
Nematode eel parasite found inside acanthocephalan cysts--a "Trojan horse" strategy?

PubMed

Emde, Sebastian; Rueckert, Sonja; Kochmann, Judith; Knopf, Klaus; Sures, Bernd; Klimpel, Sven

2014-11-18

The invasive eel parasite Anguillicoloides crassus (syn. Anguillicola crassus) is considered one of the major causes for the decline of the European eel (Anguilla anguilla) panmictic population. It impairs the swim bladder function and reduces swimming performance of its host. The life cycle of this parasite involves different intermediate and paratenic hosts. Despite an efficient immune system of the paratenic fish hosts acting against infections with A. crassus, levels of parasitized eels remain high in European river systems. Recently, the round goby Neogobius melanostomus (Gobiidae) has become dominant in many rivers in Europe and is still spreading at a rapid pace. This highly invasive species might potentially act as an important, so far neglected paratenic fish host for A. crassus. Based on own observations and earlier single sightings of A. crassus in N. melanostomus, 60 fresh individuals of N. melanostomus were caught in the Rhine River and examined to assess the infection levels with metazoan parasites, especially A. crassus. Glycerin preparations were used for parasite identification. The parasite most frequently found in N. melanostomus was the acanthocephalan Pomphorhynchus sp. (subadult stage) which occurred mainly encysted in the mesenteries and liver. Every third gobiid (P = 31.7%) was infected by A. crassus larvae (L3) which exclusively occurred inside the acanthocephalan cysts. No intact or degenerated larvae of A. crassus were detected elsewhere in the goby, neither in the body cavity and mesenteries nor in other organs. Affected cysts contained the acanthocephalan larvae and 1-12 (mI =3) living A. crassus larvae. Additionally, encysted larvae of the nematode Raphidascaris acus were detected in the gobies, but only in the body cavity and not inside the acanthocephalan cysts. Based on our observations, we suggest that A. crassus might actively bypass the immune response of N. melanostomus by invading the cysts of acanthocephalan parasites of the
Yellow fever: the recurring plague.

PubMed

Tomori, Oyewale

2004-01-01

Despite the availability of a safe and efficacious vaccine, yellow fever (YF) remains a disease of significant public health importance, with an estimated 200,000 cases and 30,000 deaths annually. The disease is endemic in tropical regions of Africa and South America; nearly 90% of YF cases and deaths occur in Africa. It is a significant hazard to unvaccinated travelers to these endemic areas. Virus transmission occurs between humans, mosquitoes, and monkeys. The mosquito, the true reservoir of YF, is infected throughout its life, and can transmit the virus transovarially through infected eggs. Man and monkeys, on the other hand, play the role of temporary amplifiers of the virus available for mosquito infection. Recent increases in the density and distribution of the urban mosquito vector, Aedes aegypti, as well as the rise in air travel increase the risk of introduction and spread of yellow fever to North and Central America, the Caribbean, the Middle East, Asia, Australia, and Oceania. It is an acute infectious disease characterized by sudden onset with a two-phase development, separated by a short period of remission. The clinical spectrum of yellow fever varies from very mild, nonspecific, febrile illness to a fulminating, sometimes fatal disease with pathognomic features. In severe cases, jaundice, bleeding diathesis, with hepatorenal involvement are common. The case fatality rate of severe yellow fever is 50% or higher. The pathogenesis and pathophysiology of the disease are poorly understood and have not been the subject of modern clinical research. There is no specific treatment for YF, making the management of YF patients extremely problematic. YF is a zoonotic disease that cannot be eradicated, therefore instituting preventive vaccination through routine childhood vaccination in endemic countries, can significantly reduce the burden of the disease. The distinctive properties of lifelong immunity after a single dose of yellow fever vaccination are the
Pressure and temperature interactions on aerobic metabolism in migrating silver eels: results in vitro.

PubMed

Scaion, D; Vettier, A; Sébert, P

2008-01-01

The European eel (Anguilla anguilla) migrates (6000 km) from European coast towards the supposed spawning area: the Sargasso Sea. This intensive and sustained swimming activity is performed without feeding and by using essentially red muscle i.e. aerobic metabolism. Temperature and hydrostatic pressure vary during migration and have known effects on energy metabolism, mainly on mitochondrial functioning. We raise the question about the existence of a pressure-temperature combination that optimizes energy metabolism. We have measured the maximal oxygen consumption (MO2) of red muscle fibres of silver eel (migrating stage) in a temperature range (5 to 25 degrees C) covering what can be reasonably expected during the migration. We have combined (random order) three temperatures (5, 15, 25 degrees C) with 5 different pressures steps from 0.1 to 10.1 MPa (corresponding to depths from surface to 1000 m). The results show that when an adequate temperature is chosen as a reference, pressure effects and pressure sensitivity depend on the temperature. Based on the fact that energy budget is limited in migrating eels, we consider that the best conditions are low temperature and high pressure.
Giant Scour on Eel Fan, California Records Decadal Trubidite Recurrence Interval

NASA Astrophysics Data System (ADS)

Paull, C. K.; McGann, M.; Edwards, B. D.; Barnes, P.; Gwiazda, R.; Lundsten, E. M.; Anderson, K.; Sumner, E.; Caress, D. W.

2013-12-01

Analysis of an exceptional exposure of thinly bedded turbidites in the headwall of a giant submarine scour on Eel Submarine Fan reveals that turbidites were being emplaced with a remarkably high frequency during the late Pleistocene to early Holocene. ROV observations, vibracores and push cores show an 87 m thick outcrop of fine-grained turbidites that are between 3 and 40 cm thick. The turbidites are composed of fining-upwards cycles grading from fine or very fine sand through to silty-mud. Within turbidites reversals in grain size occur representing fluctuating flow conditions. An abundance of woody material and shallow dwelling foraminifera within the turbidites demonstrates the flows originated in shallow water. The silty-mud horizons are barren of pelagic foraminifera, suggesting that they are the late stages of turbidites and that intervening hemipelagic material is absent. Twenty-one 14C measurements made on the transported shallow-water foraminifera and wood fragments within eighteen push cores taken from the scarp reveal that the entire 87 m section was deposited 6 to 14 thousand years BP. Shipboard video observations indicate the appearance of the scarp face is similar throughout the exposed face. In a 10 m section with excellent video, 88 turbidites were counted. Assuming that the entire 87 m face comprises the same rhythmic alterations, more than 766 turbidites were deposited during this ~8,000 year interval. This requires a decadal turbidite recurrence interval. As these deposits are downstream of the Eel River, these data have intriguing implications about the enhanced impact of the Eel River during the late Pleistocene and early Holocene.
Evaluation of Multi-Scale Climate Effects on Annual Recruitment Levels of the Japanese Eel, Anguilla japonica, to Taiwan

PubMed Central

Tzeng, Wann-Nian; Tseng, Yu-Heng; Han, Yu-San; Hsu, Chih-Chieh; Chang, Chih-Wei; Di Lorenzo, Emanuele; Hsieh, Chih-hao

2012-01-01

Long-term (1967–2008) glass eel catches were used to investigate climatic effects on the annual recruitment of Japanese eel to Taiwan. Specifically, three prevailing hypotheses that potentially explain the annual recruitment were evaluated. Hypothesis 1: high precipitation shifts the salinity front northward, resulting in favorable spawning locations. Hypothesis 2: a southward shift of the position of the North Equatorial Current (NEC) bifurcation provides a favorable larval transport route. Hypothesis 3: ocean conditions (eddy activities and productivity) along the larval migration route influence larval survival. Results of time series regression and wavelet analyses suggest that Hypothesis 1 is not supported, as the glass eel catches exhibited a negative relationship with precipitation. Hypothesis 2 is plausible. However, the catches are correlated with the NEC bifurcation with a one-year lag. Considering the time needed for larval transport (only four to six months), the one-year lag correlation does not support the direct transport hypothesis. Hypothesis 3 is supported indirectly by the results. Significant correlations were found between catches and climate indices that affect ocean productivity and eddy activities, such as the Quasi Biennial Oscillation (QBO), North Pacific Gyre Oscillation (NPGO), Pacific Decadal Oscillation (PDO), and Western Pacific Oscillation (WPO). Wavelet analysis reveals three periodicities of eel catches: 2.7, 5.4, and 10.3 years. The interannual coherence with QBO and the Niño 3.4 region suggests that the shorter-term climate variability is modulated zonally by equatorial dynamics. The low-frequency coherence with WPO, PDO, and NPGO demonstrates the decadal modulation of meridional teleconnection via ocean–atmosphere interactions. Furthermore, WPO and QBO are linked to solar activities. These results imply that the Japanese eel recruitment may be influenced by multi-timescale climate variability. Our findings call for
Evaluation of multi-scale climate effects on annual recruitment levels of the Japanese eel, Anguilla japonica, to Taiwan.

PubMed

Tzeng, Wann-Nian; Tseng, Yu-Heng; Han, Yu-San; Hsu, Chih-Chieh; Chang, Chih-Wei; Di Lorenzo, Emanuele; Hsieh, Chih-Hao

2012-01-01

Long-term (1967-2008) glass eel catches were used to investigate climatic effects on the annual recruitment of Japanese eel to Taiwan. Specifically, three prevailing hypotheses that potentially explain the annual recruitment were evaluated. Hypothesis 1: high precipitation shifts the salinity front northward, resulting in favorable spawning locations. Hypothesis 2: a southward shift of the position of the North Equatorial Current (NEC) bifurcation provides a favorable larval transport route. Hypothesis 3: ocean conditions (eddy activities and productivity) along the larval migration route influence larval survival. Results of time series regression and wavelet analyses suggest that Hypothesis 1 is not supported, as the glass eel catches exhibited a negative relationship with precipitation. Hypothesis 2 is plausible. However, the catches are correlated with the NEC bifurcation with a one-year lag. Considering the time needed for larval transport (only four to six months), the one-year lag correlation does not support the direct transport hypothesis. Hypothesis 3 is supported indirectly by the results. Significant correlations were found between catches and climate indices that affect ocean productivity and eddy activities, such as the Quasi Biennial Oscillation (QBO), North Pacific Gyre Oscillation (NPGO), Pacific Decadal Oscillation (PDO), and Western Pacific Oscillation (WPO). Wavelet analysis reveals three periodicities of eel catches: 2.7, 5.4, and 10.3 years. The interannual coherence with QBO and the Niño 3.4 region suggests that the shorter-term climate variability is modulated zonally by equatorial dynamics. The low-frequency coherence with WPO, PDO, and NPGO demonstrates the decadal modulation of meridional teleconnection via ocean-atmosphere interactions. Furthermore, WPO and QBO are linked to solar activities. These results imply that the Japanese eel recruitment may be influenced by multi-timescale climate variability. Our findings call for
Structure, properties and enhanced expression of galactose-binding C-type lectins in mucous cells of gills from freshwater Japanese eels (Anguilla japonica).

PubMed Central

Mistry, A C; Honda, S; Hirose, S

2001-01-01

Using a Japanese-eel (Anguilla japonica) gill cDNA subtraction library, two novel beta-d-galactose-binding lectins were identified that belong to group VII of the animal C-type lectin family. The eel C-type lectins, termed eCL-1 and eCL-2, are simple lectins composed of 163 amino acid residues, including a 22-residue signal peptide for secretion and a single carbohydrate-recognition domain (CRD) of approximately 130 residues typical of C-type lectins. The galactose specificity of the CRD was suggested by the presence of a QPD motif and confirmed by a competitive binding assay. Using Ruthenium Red staining, the lectins were shown to bind Ca(2+) ions. SDS/PAGE showed that native eCL-1 and eCL-2 have an SDS-resistant octameric structure (a tetramer of disulphide-linked dimers). Northern and Western blot analyses demonstrated high-level expression of eCL-1 and eCL-2 mRNAs and their protein products in gills from freshwater eels, which decreased markedly when the eels were transferred from freshwater to seawater. Immunohistochemistry showed that the eel lectins are localized in the exocrine mucous cells of the gill. PMID:11695997
Structure, properties and enhanced expression of galactose-binding C-type lectins in mucous cells of gills from freshwater Japanese eels (Anguilla japonica).

PubMed

Mistry, A C; Honda, S; Hirose, S

2001-11-15

Using a Japanese-eel (Anguilla japonica) gill cDNA subtraction library, two novel beta-d-galactose-binding lectins were identified that belong to group VII of the animal C-type lectin family. The eel C-type lectins, termed eCL-1 and eCL-2, are simple lectins composed of 163 amino acid residues, including a 22-residue signal peptide for secretion and a single carbohydrate-recognition domain (CRD) of approximately 130 residues typical of C-type lectins. The galactose specificity of the CRD was suggested by the presence of a QPD motif and confirmed by a competitive binding assay. Using Ruthenium Red staining, the lectins were shown to bind Ca(2+) ions. SDS/PAGE showed that native eCL-1 and eCL-2 have an SDS-resistant octameric structure (a tetramer of disulphide-linked dimers). Northern and Western blot analyses demonstrated high-level expression of eCL-1 and eCL-2 mRNAs and their protein products in gills from freshwater eels, which decreased markedly when the eels were transferred from freshwater to seawater. Immunohistochemistry showed that the eel lectins are localized in the exocrine mucous cells of the gill.
Fe and O EELS Studies of Ion Irradiated Murchison CM2 Carbonaceous Chondrite Matrix

NASA Technical Reports Server (NTRS)

Keller, L. P.; Christofferson, R.; Dukes, C. A.; Baragiola, R. A.; Rahman, Z.

2015-01-01

Introduction: The physical and chemical response of hydrated carbonaceous chondrite materials to space weathering processes is poorly understood. Improving this understanding is a key part of establishing how regoliths on primitive carbonaceous asteroids respond to space weathering processes, knowledge that supports future sample return missions (Hayabusa 2 and OSIRISREx) that are targeting objects of this type. We previously reported on He+ irradiation of Murchison matrix and showed that the irradiation resulted in amorphization of the matrix phyllosilicates, loss of OH, and surface vesiculation. Here, we report electron energy-loss spectroscopy (EELS) measurements of the irradiated material with emphasis on the Fe and O speciation. Sample and Methods: A polished thin section of the Murchison CM2 carbonaceous chondrite was irradiated with 4 kilovolts He(+) (normal incidence) to a total dose of 1 x 10(exp 18) He(+) per square centimeter. We extracted thin sections from both irradiated and unirradiated regions in matrix using focused ion beam (FIB) techniques with electron beam deposition for the protective carbon strap to minimize surface damage artifacts from the FIB milling. The FIB sections were analyzed using a JEOL 2500SE scanning and transmission electron microscope (STEM) equipped with a Gatan Tridiem imaging filter. EELS spectra were collected from 50 nanometer diameter regions with an energy resolution of 0.7 electronvolts FWHM at the zero loss. EELS spectra were collected at low electron doses to minimize possible artifacts from electron-beam irradiation damage. Results and Discussion: Fe L (sub 2,3) EELS spectra from matrix phyllosilicates in CM chondrites show mixed Fe(2+)/Fe(3+) oxidation states with Fe(3+)/Sigma Fe approximately 0.5. Fe L(sub 2,3) spectra from the irradiated/ amorphized matrix phyllosilicates show higher Fe(2+)/Fe(3+) ratios compared to spectra obtained from pristine material at depths beyond the implantation/amorphization layer. We
Detecting genome-wide gene transcription profiles associated with high pollution burden in the critically endangered European eel.

PubMed

Pujolar, J M; Milan, M; Marino, I A M; Capoccioni, F; Ciccotti, E; Belpaire, C; Covaci, A; Malarvannan, G; Patarnello, T; Bargelloni, L; Zane, L; Maes, G E

2013-05-15

The European eel illustrates an example of a critically endangered fish species strongly affected by human stressors throughout its life cycle, in which pollution is considered to be one of the factors responsible for the decline of the stock. The objective of our study was to better understand the transcriptional response of European eels chronically exposed to pollutants in their natural environment. A total of 42 pre-migrating (silver) female eels from lowly, highly and extremely polluted environments in Belgium and, for comparative purposes, a lowly polluted habitat in Italy were measured for polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and brominated flame retardants (BFRs). Multipollutant level of bioaccumulation was linked to their genome-wide gene transcription using an eel-specific array of 14,913 annotated cDNAs. Shared responses to pollutant exposure were observed when comparing the highly polluted site in Belgium with the relatively clean sites in Belgium and Italy. First, an altered pattern of transcription of genes was associated with detoxification, with a novel European eel CYP3A gene and gluthatione S-transferase transcriptionally up-regulated. Second, an altered pattern of transcription of genes associated with the oxidative phosphorylation pathway, with the following genes involved in the generation of ATP being transcriptionally down-regulated in individuals from the highly polluted site: NADH dehydrogenase, succinate dehydrogenase, ubiquinol-cytochrome c reductase, cytochrome c oxidase and ATP synthase. Although we did not measure metabolism directly, seeing that the transcription level of many genes encoding enzymes involved in the mitochondrial respiratory chain and oxidative phosphorylation were down-regulated in the highly polluted site suggests that pollutants may have a significant effect on energy metabolism in these fish. Copyright © 2013 Elsevier B.V. All rights reserved.
Yellow fever.

PubMed

Monath, Thomas P; Vasconcelos, Pedro F C

2015-03-01

Yellow fever, a mosquito-borne flavivirus disease occurs in tropical areas of South America and Africa. It is a disease of major historical importance, but remains a threat to travelers to and residents of endemic areas despite the availability of an effective vaccine for nearly 70 years. An important aspect is the receptivity of many non-endemic areas to introduction and spread of yellow fever. This paper reviews the clinical aspects, pathogenesis, and epidemiology of yellow fever, with an emphasis on recent changes in the distribution and incidence of the disease. Recent knowledge about yellow fever 17D vaccine mechanism of action and safety are discussed. Copyright © 2014 Elsevier B.V. All rights reserved.

Duplicated Leptin Receptors in Two Species of Eel Bring New Insights into the Evolution of the Leptin System in Vertebrates

PubMed Central

Morini, Marina; Pasquier, Jérémy; Dirks, Ron; van den Thillart, Guido; Tomkiewicz, Jonna; Rousseau, Karine; Dufour, Sylvie; Lafont, Anne-Gaëlle

2015-01-01

Since its discovery in mammals as a key-hormone in reproduction and metabolism, leptin has been identified in an increasing number of tetrapods and teleosts. Tetrapods possess only one leptin gene, while most teleosts possess two leptin genes, as a result of the teleost third whole genome duplication event (3R). Leptin acts through a specific receptor (LEPR). In the European and Japanese eels, we identified two leptin genes, and for the first time in vertebrates, two LEPR genes. Synteny analyses indicated that eel LEPRa and LEPRb result from teleost 3R. LEPRb seems to have been lost in the teleost lineage shortly after the elopomorph divergence. Quantitative PCRs revealed a wide distribution of leptins and LEPRs in the European eel, including tissues involved in metabolism and reproduction. Noticeably, leptin1 was expressed in fat tissue, while leptin2 in the liver, reflecting subfunctionalization. Four-month fasting had no impact on the expression of leptins and LEPRs in control European eels. This might be related to the remarkable adaptation of silver eel metabolism to long-term fasting throughout the reproductive oceanic migration. In contrast, sexual maturation induced differential increases in the expression of leptins and LEPRs in the BPG-liver axis. Leptin2 was strikingly upregulated in the liver, the central organ of the reproductive metabolic challenge in teleosts. LEPRs were differentially regulated during sexual maturation, which may have contributed to the conservation of the duplicated LEPRs in this species. This suggests an ancient and positive role of the leptin system in the vertebrate reproductive function. This study brings new insights on the evolutionary history of the leptin system in vertebrates. Among extant vertebrates, the eel represents a unique case of duplicated leptins and leptin receptors as a result of 3R. PMID:25946034
XPS and EELS characterization of Mn2SiO4, MnSiO3 and MnAl2O4

NASA Astrophysics Data System (ADS)

Grosvenor, A. P.; Bellhouse, E. M.; Korinek, A.; Bugnet, M.; McDermid, J. R.

2016-08-01

X-ray Photoelectron Spectroscopy (XPS) and Electron Energy Loss Spectroscopy (EELS) are strong candidate techniques for characterizing steel surfaces and substrate-coating interfaces when investigating the selective oxidation and reactive wetting of advanced high strength steels (AHSS) during the continuous galvanizing process. However, unambiguous identification of ternary oxides such as Mn2SiO4, MnSiO3, and MnAl2O4 by XPS or EELS, which can play a significant role in substrate reactive wetting, is difficult due to the lack of fully characterized standards in the literature. To resolve this issue, samples of Mn2SiO4, MnSiO3 and MnAl2O4 were synthesized and characterized by XPS and EELS. The unique features of the XPS and EELS spectra for the Mn2SiO4, MnSiO3 and MnAl2O4 standards were successfully derived, thereby allowing investigators to fully differentiate and identify these oxides at the surface and subsurface of Mn, Si and Al alloyed AHSS using these techniques.
Ammonia stress under high environmental ammonia induces Hsp70 and Hsp90 in the mud eel, Monopterus cuchia.

PubMed

Hangzo, Hnunlalliani; Banerjee, Bodhisattwa; Saha, Shrabani; Saha, Nirmalendu

2017-02-01

The obligatory air-breathing mud eel (Monopterus cuchia) is frequently being challenged with high environmental ammonia (HEA) exposure in its natural habitats. The present study investigated the possible induction of heat shock protein 70 and 90 (hsp70, hsc70, hsp90α and hsp90β) genes and more expression of Hsp70 and Hsp90 proteins under ammonia stress in different tissues of the mud eel after exposure to HEA (50 mM NH 4 Cl) for 14 days. HEA resulted in significant accumulation of toxic ammonia in different body tissues and plasma, which was accompanied with the stimulation of oxidative stress in the mud eel as evidenced by more accumulation of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) during exposure to HEA. Further, hyper-ammonia stress led to significant increase in the levels of mRNA transcripts for inducible hsp70 and hsp90α genes and also their translated proteins in different tissues probably as a consequence of induction of hsp70 and hsp90α genes in the mud eel. However, hyper-ammonia stress was neither associated with any significant alterations in the levels of mRNA transcripts for constitutive hsc70 and hsp90β genes nor their translated proteins in any of the tissues studied. More abundance of Hsp70 and Hsp90α proteins might be one of the strategies adopted by the mud eel to defend itself from the ammonia-induced cellular damages under ammonia stress. Further, this is the first report of ammonia-induced induction of hsp70 and hsp90α genes under hyper-ammonia stress in any freshwater air-breathing teleost.
Acoustic backscatter of the 1995 flood deposit on the Eel shelf

USGS Publications Warehouse

Borgeld, J.C.; Hughes-Clarke, John E.; Goff, John A.; Mayer, Larry A.; Curtis, Jennifer A.

1999-01-01

Acoustic swath mapping and sediment box coring conducted on the continental shelf near the mouth of the Eel River revealed regional variations in acoustic backscatter that can be related to the shelf sedimentology. The acoustic-backscatter variations observed on the shelf were unusually narrow compared to the response of similar sediment types documented in other areas. However, the acoustic data revealed four principal bottom types on the shelf that can be related to sedimentologic differences observed in cores. The four areas are: (1) low acoustic backscatter associated with the nearshore-sand facies and the prodelta terraces of the Eel and Mad rivers, composed of fine sands and coarse silts with low porosity; (2) high acoustic backscatter associated with fine silts characterized by high porosity and deposited by the 1995 flood of the Eel River; (3) intermediate acoustic backscatter in the outer-shelf muds, where clayey silts are accumulating and the 1995 flood apparently had limited direct effect; and (4) intermediate acoustic backscatter near the fringes of the 1995 flood deposits and in areas where the flood sediments were more disrupted by post-depositional processes. The highest acoustic backscatter was identified in areas where the 1995 flood sediments remained relatively intact and near the shelf surface into the summer of 1995. Cores collected from these areas contained wavy or lenticular bedding. The rapid deposition of the high-porosity muddy layers results in better preservation of incorporated ripple forms than in areas less directly impacted by the flood deposit. The high-porosity muddy layers allow acoustic penetration into the sediments and result in greater acoustic backscatter from incorporated roughness elements.
Flight feather molt in Yellow-headed Blackbirds (Xanthocephalus xanthocephalus) in North Dakota

USGS Publications Warehouse

Twedt, Daniel J.; Linz, George M.

2015-01-01

Yellow-headed Blackbirds (Xanthocephalus xanthocephalus) in central North Dakota undergo prebasic molt or prejuvenile molt during late summer. Nestling Yellow-headed Blackbirds initiate a complete prejuvenile molt, grow their primary and secondary regimes in about 40 days, completing molt after they leave the nest by the first week in August. Remiges are not replaced during the subsequent preformative molt, being retained until the second prebasic molt. Nonlinear (logistic) regression of primary remex growth during definitive prebasic molts of Yellow-headed Blackbirds indicated 38 days were required to complete the linear phase of growth (between 10% and 90% of total primary length). Males added 19.5 mm/d and females added 15.7 mm/d to the total length of all primaries during this linear growth phase; an average of 4–5 mm per primary remex per day. Definitive prebasic molting of primary remiges in males and females was initiated in late June, after nesting and brood rearing were completed. Molts of Yellow-headed Blackbirds were completed by early September, before birds emigrated from North Dakota during mid-September. Because of their comparatively early completion of molt and emigration from the state, as well as their more diverse diet, agricultural depredation caused by Yellow-headed Blackbirds in North Dakota is likely less than that of Red-winged Blackbirds and Common Grackles.
Molecular phylogenetics reveals convergent evolution in lower Congo River spiny eels.

PubMed

Alter, S Elizabeth; Brown, Bianca; Stiassny, Melanie L J

2015-10-15

The lower Congo River (LCR) is a region of exceptional species diversity and endemism in the Congo basin, including numerous species of spiny eels (genus Mastacembelus). Four of these exhibit distinctive phenotypes characterized by greatly reduced optic globes deeply embedded into the head (cryptophthalmia) and reduced (or absent) melanin pigmentation, among other characteristics. A strikingly similar cryptophthalmic phenotype is also found in members of a number of unrelated fish families, strongly suggesting the possibility of convergent evolution. However, little is known about the evolutionary processes that shaped diversification in LCR Mastacembelus, their biogeographic origins, or when colonization of the LCR occurred. We sequenced mitochondrial and nuclear genes from Mastacembelus species collected in the lower Congo River, and compared them with other African species and Asian representatives as outgroups. We analyzed the sequence data using Maximum Likelihood and Bayesian phylogenetic inference. Bayesian and Maximum Likelihood phylogenetic analyses, and Bayesian coalescent methods for species tree reconstruction, reveal that endemic LCR spiny eels derive from two independent origins, clearly demonstrating convergent evolution of the cryptophthalmic phenotype. Mastacembelus crassus, M. aviceps, and M. simbi form a clade, allied to species found in southern, eastern and central Africa. Unexpectedly, M. brichardi and brachyrhinus fall within a clade otherwise endemic to Lake Tanganikya (LT) ca. 1500 km east of the LCR. Divergence dating suggests the ages of these two clades of LCR endemics differ markedly. The age of the crassus group is estimated at ~4 Myr while colonization of the LCR by the brichardi-brachyrhinus progenitor was considerably more recent, dated at ~0.5 Myr. The phylogenetic framework of spiny eels presented here, the first to include LCR species, demonstrates that cryptophthalmia and associated traits evolved at least twice in Mastacembelus
Dr. Alexander Garden, a Linnaean in colonial America, and the saga of five "electric eels".

PubMed

Finger, Stanley

2010-01-01

Alexander Garden (1730-1791) was born in Scotland, where he trained in medicine before settling in South Carolina in 1752. With a passion for collecting and a love of nature, he sent specimens to Linnaeus and his associates in Europe. In 1774, Garden observed and conducted electrical experiments on some "eels" that had survived the trip from Surinam to Charleston. His detailed observations and reasons for believing they emit electricity were read before the Royal Society of London and subsequently published. He also advised the sea captain who owned the eels on how to preserve them and where to deliver their bodies if they died en route to London. Although they did die en route, John Hunter of the Royal Society was able to provide excellent descriptions of their electric organs because they were so well preserved. The sequence of events that followed led to other live eels surviving the voyage to London, where they were observed to spark in darkness in 1776. This was a critical piece of evidence in favor of fish electricity and eventually for the more revolutionary idea that even human nerves and muscles might function electrically.
Long-term stability in the richness and structure of helminth communities in eels, Anguilla anguilla, in Lough Derg, River Shannon, Ireland.

PubMed

Kennedy, C R; Moriarty, C

2002-12-01

A data set on intestinal helminth parasites was collected in the course of an 18 year investigation into the biology of eels in Meelick Bay, Lough Derg, River Shannon. This was used to test two hypotheses relating to the composition and structure of intestinal helminth communities, namely that eels in large rivers do not harbour richer and more diverse communities than those in small rivers but that community composition and structure are more stable over time than in small rivers. The helminth community was species poor, with only six species comprising the component community and a maximum infracommunity richness of three species. The community was overwhelmingly dominated by the acanthocephalan Acanthocephalus lucii, reflecting the importance of its intermediate host Asellus aquaticus in the eels' diet. The remaining helminth species contributed to species richness but made very little contribution to community diversity. Population levels of Acanthocephalus lucii fell and remained low between 1992 and 2000, probably reflecting increased movement of eels from other parts of the lough into Meelick Bay. Diversity values were low, but similar to those reported from other rivers in Britain and Europe. The results provided support for both hypotheses and indicated that in respect of richness, diversity and dominance, the helminth communities of eels in the River Shannon were typical of, and comparable to, those of other large rivers throughout Europe.
First Report of ‘Candidatus Phytoplasma trifolii’-Related Strain of 16SrVI-A Phytoplasma Subgroup, Associated with Elm Yellows Disease in American Elm ( Ulmus americana L.) in Ohio, U.S.A

Treesearch

C.E. Flower; N. Hayes-Plazolles; J.M. Slavicek; C. Rosa

2018-01-01

During the investigation of the sudden and early onset of yellowing and mortality of American elm (Ulmus americana L.) trees at the USDA Forest Service Northern Research Station in Delaware, OH, a phytoplasma of the clover proliferation group (16SrVI) was detected as the putative causal agent of the disease outbreak.
Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment.

PubMed

Gruber, David F; Gaffney, Jean P; Mehr, Shaadi; DeSalle, Rob; Sparks, John S; Platisa, Jelena; Pieribone, Vincent A

2015-01-01

We report the identification and characterization of two new members of a family of bilirubin-inducible fluorescent proteins (FPs) from marine chlopsid eels and demonstrate a key region of the sequence that serves as an evolutionary switch from non-fluorescent to fluorescent fatty acid-binding proteins (FABPs). Using transcriptomic analysis of two species of brightly fluorescent Kaupichthys eels (Kaupichthys hyoproroides and Kaupichthys n. sp.), two new FPs were identified, cloned and characterized (Chlopsid FP I and Chlopsid FP II). We then performed phylogenetic analysis on 210 FABPs, spanning 16 vertebrate orders, and including 163 vertebrate taxa. We show that the fluorescent FPs diverged as a protein family and are the sister group to brain FABPs. Our results indicate that the evolution of this family involved at least three gene duplication events. We show that fluorescent FABPs possess a unique, conserved tripeptide Gly-Pro-Pro sequence motif, which is not found in non-fluorescent fatty acid binding proteins. This motif arose from a duplication event of the FABP brain isoforms and was under strong purifying selection, leading to the classification of this new FP family. Residues adjacent to the motif are under strong positive selection, suggesting a further refinement of the eel protein's fluorescent properties. We present a phylogenetic reconstruction of this emerging FP family and describe additional fluorescent FABP members from groups of distantly related eels. The elucidation of this class of fish FPs with diverse properties provides new templates for the development of protein-based fluorescent tools. The evolutionary adaptation from fatty acid-binding proteins to fluorescent fatty acid-binding proteins raises intrigue as to the functional role of bright green fluorescence in this cryptic genus of reclusive eels that inhabit a blue, nearly monochromatic, marine environment.
Effect of TBT and PAHs on CYP1A, AhR and Vitellogenin Gene Expression in the Japanese Eel, Anguilla japonica.

PubMed

Choi, Min Seop; Kwon, Se Ryun; Choi, Seong Hee; Kwon, Hyuk Chu

2012-12-01

Gene expressions of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor (AhR) and vitellogenin (Vg) by endocrine disruptors, benzo[α]pyrene (B[a]P) and tributyltin (TBT) were examined in cultured eel hepatocytes which were isolated from eels treated previously with B[a]P (10 mg/kg) or estradiol-17β (20 mg/kg) in vivo, and the relationship between CYP1A, AhR and Vg genes were studied. When the cultured eel hepatocytes were treated with B[a]P (10(-6)-10(-5) M) the gene expressions of CYP1A and AhR were enhanced in a concentration-dependent manner. However, when treated with TBT (10(-9)-10(-5) M) the gene expressions of CYP1A and AhR were suppressed at high concentrations (10(-6)-10(-5) M), while having no effects at low concentrations (10(-9)-10(-7) M). Gene expression of Vg was also suppressed by TBT in a concentration-dependent manner in cultured eel hepatocytes which was previously treated in vivo with estradiol-17β.
Effect of TBT and PAHs on CYP1A, AhR and Vitellogenin Gene Expression in the Japanese Eel, Anguilla japonica

PubMed Central

Choi, Min Seop; Kwon, Se Ryun; Choi, Seong Hee; Kwon, Hyuk Chu

2012-01-01

Gene expressions of cytochrome P4501A (CYP1A), aryl hydrocarbon receptor (AhR) and vitellogenin (Vg) by endocrine disruptors, benzo[α]pyrene (B[a]P) and tributyltin (TBT) were examined in cultured eel hepatocytes which were isolated from eels treated previously with B[a]P (10 mg/kg) or estradiol-17β (20 mg/kg) in vivo, and the relationship between CYP1A, AhR and Vg genes were studied. When the cultured eel hepatocytes were treated with B[a]P (10-6-10-5 M) the gene expressions of CYP1A and AhR were enhanced in a concentration-dependent manner. However, when treated with TBT (10-9-10-5 M) the gene expressions of CYP1A and AhR were suppressed at high concentrations (10-6-10-5 M), while having no effects at low concentrations (10-9-10-7 M). Gene expression of Vg was also suppressed by TBT in a concentration-dependent manner in cultured eel hepatocytes which was previously treated in vivo with estradiol-17β. PMID:25949102
Identification of Drosophila melanogaster yellow-f and yellow-f2 proteins as dopachrome-conversion enzymes.

PubMed Central

Han, Qian; Fang, Jianmin; Ding, Haizhen; Johnson, Jody K; Christensen, Bruce M; Li, Jianyong

2002-01-01

This study describes the identification of Drosophila yellow-f and yellow-f2 as dopachrome-conversion enzymes responsible for catalysing the conversion of dopachrome into 5,6-dihydroxyindole in the melanization pathway. Drosophila yellow -y gene and yellow -b, -c, -f and -f2 genes were expressed in an insect cell/baculovirus expression system and their corresponding recombinant proteins were screened for dopachrome-conversion enzyme activity. Among the yellow and yellow -related genes, the yellow -f and yellow -f2 genes were identified as the genes coding for Drosophila dopachrome-conversion enzyme based on the high activity of their recombinant proteins in catalysing the production of 5,6-dihydroxyindole from dopachrome. Both yellow-f and yellow-f2 are capable of mediating a decarboxylative structural rearrangement of dopachrome, as well as an isomerization/tautomerization of dopamine chrome and dopa methyl ester chrome. Northern hybridization revealed the transcription of yellow -f in larvae and pupae, but a high abundance of mRNA was observed in later larval and early pupal stages. In contrast, yellow-f2 transcripts were present at all stages, but high abundance of its mRNA was observed in later-stage pupae and adults. These data indicate that yellow-f and yellow-f2 complement each other during Drosophila development and that the yellow-f is involved in larval and pupal melanization, and yellow-f2 plays a major role in melanization reactions in Drosophila during later pupal and adult development. Results from this study provide the groundwork towards a better understanding of the physiological roles of the Drosophila yellow gene family. PMID:12164780
YELLOW SUPERGIANTS IN THE ANDROMEDA GALAXY (M31)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Drout, Maria R.; Massey, Philip; Meynet, Georges

2009-09-20

The yellow supergiant content of nearby galaxies can provide a critical test of stellar evolution theory, bridging the gap between the hot, massive stars and the cool red supergiants. But, this region of the color-magnitude diagram is dominated by foreground contamination, requiring membership to somehow be determined. Fortunately, the large negative systemic velocity of M31, coupled to its high rotation rate, provides the means for separating the contaminating foreground dwarfs from the bona fide yellow supergiants within M31. We obtained radial velocities of {approx}2900 individual targets within the correct color-magnitude range corresponding to masses of 12 M{sub sun} and higher.more » A comparison of these velocities to those expected from M31's rotation curve reveals 54 rank-1 (near certain) and 66 rank-2 (probable) yellow supergiant members, indicating a foreground contamination >= 96%. We expect some modest contamination from Milky Way halo giants among the remainder, particularly for the rank-2 candidates, and indeed follow-up spectroscopy of a small sample eliminates four rank 2's while confirming five others. We find excellent agreement between the location of yellow supergiants in the H-R diagram and that predicted by the latest Geneva evolutionary tracks that include rotation. However, the relative number of yellow supergiants seen as a function of mass varies from that predicted by the models by a factor of >10, in the sense that more high-mass yellow supergiants are predicted than those are actually observed. Comparing the total number (16) of >20 M{sub sun} yellow supergiants with the estimated number (24,800) of unevolved O stars indicates that the duration of the yellow supergiant phase is {approx}3000 years. This is consistent with what the 12 M{sub sun} and 15 M{sub sun} evolutionary tracks predict, but disagrees with the 20,000-80,000 year timescales predicted by the models for higher masses.« less
Black on Yellow: Afro-Americans View Chinese-Americans, 1850-1935

ERIC Educational Resources Information Center

Shankman, Arnold

1978-01-01

Using data collected from black newspapers, an explanation is sought for the unfavorable stereotypes blacks have held about Chinese Americans. Economic competition, cultural and religious differences, and blacks' belief that the Chinese were prejudiced against them are some of the historical factors mentioned which influenced black attitudes…
Energy-filtered TEM imaging and EELS study of ODS particles and argon-filled cavities in ferritic-martensitic steels.

PubMed

Klimiankou, M; Lindau, R; Möslang, A

2005-01-01

Oxide-dispersion-strengthened (ODS) ferritic-martensitic steels with yttrium oxide (Y(2)O(3)) have been produced by mechanical alloying and hot isostatic pressing for use as advanced material in fusion power reactors. Argon gas, usually widely used as inert gas during mechanical alloying, was surprisingly detected in the nanodispersion-strengthened materials. Energy-filtered transmission electron microscopy (EFTEM) and electron energy loss spectroscopy (EELS) led to the following results: (i) chemical composition of ODS particles, (ii) voids with typical diameters of 1-6 nm are formed in the matrix, (iii) these voids are filled with Ar gas, and (iv) the high-density nanosized ODS particles serve as trapping centers for the Ar bubbles. The Ar L(3,2) energy loss edge at 245 eV as well as the absorption features of the ODS particle elements were identified in the EELS spectrum. The energy resolution in the EEL spectrum of about 1.0 eV allows to identify the electronic structure of the ODS particles.
Anguilla rostrata glass eel migration and recruitment in the estuary and Gulf of St Lawrence.

PubMed

Dutil, J-D; Dumont, P; Cairns, D K; Galbraith, P S; Verreault, G; Castonguay, M; Proulx, S

2009-06-01

This study describes catches of Anguilla rostrata glass eels and associated oceanographic conditions in the St Lawrence Estuary and Gulf. Ichthyoplankton survey data suggest that they enter the Gulf primarily in May, migrate at the surface at night, and disperse broadly once they have passed Cabot Strait. They arrive in estuaries beginning at about mid-June and through the month of July. Migration extends west up to Québec City, in the freshwater zone of the St Lawrence Estuary, 1000 km west of Cabot Strait. Anguilla rostrata glass eels travel between Cabot Strait and receiving estuaries at a straight-line ground speed of c. 10-15 km day(-1). Catches of fish per unit effort in estuaries in the St Lawrence system are much lower than those reported for the Atlantic coast of Canada. Low abundance of A. rostrata glass eels in the St Lawrence system may be due to cold surface temperatures during the migration period which decrease swimming capacity, long distances from the spawning ground to Cabot Strait and from Cabot Strait to the destination waters (especially the St Lawrence River), complex circulation patterns, and hypoxic conditions in bottom waters of the Laurentian Channel and the St Lawrence Estuary.
Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel.

PubMed

Schmitz, Monika; Aroua, Salima; Vidal, Bernadette; Le Belle, Nadine; Elie, Pierre; Dufour, Sylvie

2005-01-01

Pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are, in teleosts as in mammals, under the control of hypothalamic factors and steroid feedbacks. In teleosts, feedback regulations largely vary depending on species and physiological stage. In the present study the regulation of FSH and LH expression was investigated in the European eel, a fish of biological and phylogenetical interest as a representative of an early group of teleosts. The eel FSHbeta subunit was cloned, sequenced and together with earlier isolated eel LHbeta and glycoprotein hormone alpha (GPalpha) subunits used to study the differential regulation of LH and FSH. In situ hybridization indicated that FSHbeta and LHbeta are expressed by separate cells of the proximal pars distalis of the adenohypophysis, differently from the situation in mammals. The profiles of LHbeta and FSHbeta subunit expression were compared during experimental ovarian maturation, using dot-blot assays. Expression levels for LHbeta and GPalpha increased throughout ovarian development with a positive correlation between these two subunits. Conversely, FSHbeta mRNA levels decreased. To understand the role of sex steroids in these opposite variations, immature eels were treated with estradiol (E2)and testosterone (T), both steroids being produced in eel ovaries during gonadal development. E2 treatment induced increases in both LHbeta and GPalpha mRNA levels, without any significant effect on FSHbeta. In contrast, T treatment induced a decrease in FSHbeta mRNA levels, without any significant effect on the other subunits. These data demonstrate that steroids exert a differential feedback on eel gonadotropin expression, with an E2-specific positive feedback on LH and a T-specific negative feedback on FSH, leading to an opposite regulation of LH and FSH during ovarian development. Copyright 2005 S. Karger AG, Basel
Simultaneous Determination of Potassium Sorbate, Sodium Benzoate, Quinoline Yellow and Sunset Yellow in Lemonades and Lemon Sauces by HPLC Using Experimental Design.

PubMed

Dinç Zor, Şule; Aşçı, Bürge; Aksu Dönmez, Özlem; Yıldırım Küçükkaraca, Dilek

2016-07-01

In this study, development and validation of a HPLC method was described for simultaneous determination of potassium sorbate, sodium benzoate, quinoline yellow and sunset yellow. A Box-Behnken design using three variables at three levels was employed to determine the optimum conditions of chromatographic separation: pH of mobile phase, 6.0-7.0; flow rate, 0.8-1.2 mL min(-1) and the ratio of mobile phase composed of a 0.025 M sodium acetate/acetic acid buffer, 80-90%. Resolution was chosen as a response. The optimized method was validated for linearity, the limits of detection and quantification, accuracy, precision and stability. All the validation parameters were within the acceptance range. The applicability of the developed method to the determination of these food additives in commercial lemonade and lemon sauce samples was successfully demonstrated. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.
The centennial of the Yellow Fever Commission and the use of informed consent in medical research.

PubMed

Güereña-Burgueño, Fernando

2002-01-01

The year 2000 marked the centennial of the discovery of the mode of transmission of yellow fever. Informed consent was systematically used for the first time in research. This process was the result of a complex social phenomenon involving the American Public Health Association, the US and Spanish Governments, American and Cuban scientists, the media, and civilian and military volunteers. The public health and medical communities face the AIDS pandemic at the beginning of the 21st Century, as they faced the yellow fever epidemic at the beginning of the 20th Century. Current medical research dilemmas have fueled the debate about the ethical conduct of research in human subjects. The AIDS pandemic is imposing enormous new ethical challenges on the conduct of medical research, especially in the developing world. Reflecting on the yellow fever experiments of 1900, lessons can be learned and applied to the current ethical challenges faced by the international public health research community. The English version of this paper is available too at: http://www.insp.mx/salud/index.html.

Perinatal programming of depressive-like behavior by inflammation in adult offspring mice whose mothers were fed polluted eels: Gender selective effects.

PubMed

Soualeh, Nidhal; Dridi, Imen; Eppe, Gauthier; Némos, Christophe; Soulimani, Rachid; Bouayed, Jaouad

2017-07-01

Several lines of evidence indicate that early-life inflammation may predispose to mental illness, including depression, in later-life. We investigated the impact of perinatal exposure to polluted eels on neonatal, postnatal, and adult brain inflammation, and on the resignation behavior of male and female adult offspring mice. The effects of maternal standard diet (laboratory food) were compared to the same diet enriched with low, intermediate, or highly polluted eels. Brain inflammatory markers including cytokines were assessed in offspring mice on the day of birth (i.e., on the postnatal day-PND 1), upon weaning (PND 21) and at adulthood (PND 100). Plasma myeloperoxidase and corticosterone levels were evaluated at PND 100. Immobility behavior of offspring was assessed in adulthood (i.e., at PNDs 95-100), using the tail suspension and forced swimming tests. Chronic brain inflammation was found in male and female offspring mice compared to controls, as assessed at PNDs 1, 21, and 100. The level of myeloperoxidase was found to be significantly higher in both adult males and females vs. control offspring. However, high corticosterone levels were only found in male offspring mice that were perinatally exposed to eels, suggesting a gender-selective dysregulation of the adult hypothalamic-pituitaryadrenal (HPA) axis. Gender-specific differences were also detected in adulthood in regard to offspring resignation behavior. Thus, compared to controls, males, but not females, whose mothers were fed eels during pregnancy and lactation exhibited a depressive-like behavior in adult age in both behavioral models of depression. Depressive symptoms were more pronounced in male mice perinatally exposed to either intermediate or highly polluted eels than those exposed to only lowly polluted eels. Our results indicate that early-life inflammatory insult is a plausible causative factor that induces the depressive phenotype exhibited by male adult offspring mice, most likely through a
Spectrum image analysis tool - A flexible MATLAB solution to analyze EEL and CL spectrum images.

PubMed

Schmidt, Franz-Philipp; Hofer, Ferdinand; Krenn, Joachim R

2017-02-01

Spectrum imaging techniques, gaining simultaneously structural (image) and spectroscopic data, require appropriate and careful processing to extract information of the dataset. In this article we introduce a MATLAB based software that uses three dimensional data (EEL/CL spectrum image in dm3 format (Gatan Inc.'s DigitalMicrograph ® )) as input. A graphical user interface enables a fast and easy mapping of spectral dependent images and position dependent spectra. First, data processing such as background subtraction, deconvolution and denoising, second, multiple display options including an EEL/CL moviemaker and, third, the applicability on a large amount of data sets with a small work load makes this program an interesting tool to visualize otherwise hidden details. Copyright © 2016 Elsevier Ltd. All rights reserved.
An electric-eel-inspired soft power source from stacked hydrogels

NASA Astrophysics Data System (ADS)

Schroeder, Thomas B. H.; Guha, Anirvan; Lamoureux, Aaron; Vanrenterghem, Gloria; Sept, David; Shtein, Max; Yang, Jerry; Mayer, Michael

2017-12-01

Progress towards the integration of technology into living organisms requires electrical power sources that are biocompatible, mechanically flexible, and able to harness the chemical energy available inside biological systems. Conventional batteries were not designed with these criteria in mind. The electric organ of the knifefish Electrophorus electricus (commonly known as the electric eel) is, however, an example of an electrical power source that operates within biological constraints while featuring power characteristics that include peak potential differences of 600 volts and currents of 1 ampere. Here we introduce an electric-eel-inspired power concept that uses gradients of ions between miniature polyacrylamide hydrogel compartments bounded by a repeating sequence of cation- and anion-selective hydrogel membranes. The system uses a scalable stacking or folding geometry that generates 110 volts at open circuit or 27 milliwatts per square metre per gel cell upon simultaneous, self-registered mechanical contact activation of thousands of gel compartments in series while circumventing power dissipation before contact. Unlike typical batteries, these systems are soft, flexible, transparent, and potentially biocompatible. These characteristics suggest that artificial electric organs could be used to power next-generation implant materials such as pacemakers, implantable sensors, or prosthetic devices in hybrids of living and non-living systems.
An electric-eel-inspired soft power source from stacked hydrogels.

PubMed

Schroeder, Thomas B H; Guha, Anirvan; Lamoureux, Aaron; VanRenterghem, Gloria; Sept, David; Shtein, Max; Yang, Jerry; Mayer, Michael

2017-12-13

Progress towards the integration of technology into living organisms requires electrical power sources that are biocompatible, mechanically flexible, and able to harness the chemical energy available inside biological systems. Conventional batteries were not designed with these criteria in mind. The electric organ of the knifefish Electrophorus electricus (commonly known as the electric eel) is, however, an example of an electrical power source that operates within biological constraints while featuring power characteristics that include peak potential differences of 600 volts and currents of 1 ampere. Here we introduce an electric-eel-inspired power concept that uses gradients of ions between miniature polyacrylamide hydrogel compartments bounded by a repeating sequence of cation- and anion-selective hydrogel membranes. The system uses a scalable stacking or folding geometry that generates 110 volts at open circuit or 27 milliwatts per square metre per gel cell upon simultaneous, self-registered mechanical contact activation of thousands of gel compartments in series while circumventing power dissipation before contact. Unlike typical batteries, these systems are soft, flexible, transparent, and potentially biocompatible. These characteristics suggest that artificial electric organs could be used to power next-generation implant materials such as pacemakers, implantable sensors, or prosthetic devices in hybrids of living and non-living systems.
Yellow fever: epidemiology and prevention.

PubMed

Barnett, Elizabeth D

2007-03-15

Yellow fever continues to occur in regions of Africa and South America, despite the availability of effective vaccines. Recently, some cases of severe neurologic disease and multiorgan system disease have been described in individuals who received yellow fever vaccine. These events have focused attention on the need to define criteria for judicious use of yellow fever vaccine and to describe the spectrum of adverse events that may be associated with yellow fever vaccine. Describing host factors that would increase risk of these events and identifying potential treatment modalities for yellow fever and yellow fever vaccine-associated adverse events are subjects of intense investigation.
Maximal oxygen consumption increases with temperature in the European eel (Anguilla anguilla) through increased heart rate and arteriovenous extraction.

PubMed

Claësson, Débora; Wang, Tobias; Malte, Hans

2016-01-01

Global warming results in increasing water temperature, which may represent a threat to aquatic ectotherms. The rising temperature affects ecology through physiology, by exerting a direct limiting effect on the individual. The mechanism controlling individual thermal tolerance is still elusive, but some evidence shows that the heart plays a central role, and that insufficient transport of oxygen to the respiring tissues may determine the thermal tolerance of animals. In this study, the influence of the heart in thermal limitation was investigated by measurements of aerobic scope in the European eel ( Anguilla anguilla ) together with measurements of cardiac output during rest and activity. Aerobic capacity was not limited by an acutely increased temperature in the European eel. Oxygen demand was met by an increase in heart rate and arteriovenous extraction. These findings suggest that thermal tolerance during exposure to acute temperature changes is not defined by oxygen transport capacity in the eel, and other mechanisms may play a central role in limiting thermal tolerance in these fish.
Archaeological Survey of the Cottonwood, Redwood, and Yellow Medicine Drainages in Southwestern Minnesota. Phase 1

DTIC Science & Technology

1989-03-01

AND YELLOW MEDiCINE DRAINAGES IN SOUTHWESTERN MINNESOTA. 12 PERSONAL AUTHOR(S) Clark A. Dobbs 13a TYPE OF REPORT 13b TIME COVERED 14 DATE OF REPORT...Between April 15 and June 15, a three person field team conducted the archaeological survey in the Cottonwood, Redwood, and Yellow Medicine subbasins...sites that are exposed on the ground surface. A three- person survey team can examine between 100 and 150 acres per day, dp ending on field conditions and
February 10, 2011, Phase Two of the Yellow Bluff Air Monitoring Study - Analytical Datasheets for Corrosion Classification Coupons

EPA Pesticide Factsheets

Contains datasheets of results of Yellow Bluff Corrosion Classification Coupon Study. Monitoring was performed at the Primary, Background, and five additional residential Yellow Bluff sites from January 24-28, 2011.
Relationship between amino acid changes in mitochondrial ATP6 and life-history variation in anguillid eels.

PubMed

Jacobsen, Magnus W; Pujolar, José Martin; Hansen, Michael M

2015-03-01

Mitochondrial genes are part of the oxidative phosphorylation pathway and important for energy production. Although evidence for positive selection at the mitochondrial level exists, few studies have investigated the link between amino acid changes and phenotype. Here we test the hypothesis that differences in two life-history related traits, migratory distance between spawning and foraging areas and larval phase duration, are associated with divergent selection within the mitochondrial ATP6 gene in anguillid eels. We compare amino acid changes among 18 species with the sequence of the putative ancestral species, believed to have shown short migratory distance and larval phase duration. We find positive correlations between both life-history related traits and (i) the number of amino acid changes and (ii) the strength of the combined physico-chemical and structural changes at positions previously identified as candidates for positive selection. This supports a link between genotype and phenotype driven by positive selection at ATP6. © 2015 The Author(s) Published by the Royal Society. All rights reserved.
Functional Analysis of Promoter Region from Eel Cytochrome P450 1A1 Gene in Transgenic Medaka.

PubMed

Ogino; Itakura; Kato; Aoki; Sato

1999-07-01

: Transcription of the CYP1A1 genes in mammals and fish is stimulated by polyaromatic hydrocarbons. DNA sequencing analysis revealed that CYP1A1 gene in eel (Anguilla japonica) contains two kinds of putative cis-acting regulatory elements, XRE (xenobiotic-responsive element) and ERE (estrogen-responsive element). XRE is known as the enhancer that is responsible for the inducibility of the genes of CYP1A1 and some other drug-metabolizing enzymes. In the eel CYP1A1 gene, XRE motifs are distributed as follows: five times in the region from -2136 to -1125 bp, XRE(-6) to (-2); once in the proximal basal promoter region, XRE(-1); and once in the first intron, XRE(+1). The region between XRE(-2) and XRE(-1) contains three ERE motifs. To investigate the function of the cis-acting regulatory elements in the eel CYP1A1 gene, recombinant plasmids prepared with its 5' upstream sequence and the structural gene for luciferase were microinjected into fertilized eggs of medaka at the one-cell stage. Hatched fry were treated with 3-methylcholanthrene, and the transcription efficiency was assayed using competitive polymerase chain reaction analysis. Deletion of the region containing the five XREs, XRE(-6) to XRE(-2), and the point mutation of XRE(-1) reduced the inducible expressions by 75% and 56%, respectively, showing apparent dependency of the drug induction on the XREs. Constitutive expression, however, was not significantly affected by deletion or disruption of the XREs. When the region between XRE(-2) and XRE(-1) containing no XREs but three ERE motifs was internally deleted, the inducible expression and the constitutive expression were reduced by 88% and 75%, respectively. Replacement of this region with a partial fragment of eel CYP1A1 complementary DNA, with slight alteration of the distance between the five XREs and XRE(-1), reduced the inducible expression and the constitutive expression by 91% and 60%, respectively. These results strongly suggest that not only XRE but
January 18, 2011, Phase One of the Yellow Bluff Air Monitoring Study - Analytical Datasheets for Corrosion Classification Coupons

EPA Pesticide Factsheets

Contains datasheets of results of Yellow Bluff Corrosion Classification Coupon Study. Monitoring was performed at the Primary, Background, and five additional residential Yellow Bluff sites from November 16, 2010 - December 16, 2010.
Assessing the risk of crash for trucks on onset yellow.

DOT National Transportation Integrated Search

2012-06-01

Each day, millions of signal changes to the yellow phase occur at isolated high speed intersections, when erroneous : driver decisions to stop or go may often lead to a crash. Dilemma zone protection systems are typically used to control : these inte...
The Eel River, northwestern California; high sediment yields from a dynamic landscape

Treesearch

Thomas E. Lisle

1990-01-01

The Eel River draining the Coast Range of northwestern California has the highest recorded average suspended sediment yield per drainage area of any river of its size or larger unaffected by volcanic eruptions or active glaciers in the conterminous United States (1,720 t/km 2 yr from 9,390 km 2 ; Brown and Ritter, 1971).
De Novo Assembly of the Whole Transcriptome of the Wild Embryo, Preleptocephalus, Leptocephalus, and Glass Eel of Anguilla japonica and Deciphering the Digestive and Absorptive Capacities during Early Development.

PubMed

Hsu, Hsiang-Yi; Chen, Shu-Hwa; Cha, Yuh-Ru; Tsukamoto, Katsumi; Lin, Chung-Yen; Han, Yu-San

2015-01-01

Natural stocks of Japanese eel (Anguilla japonica) have decreased drastically because of overfishing, habitat destruction, and changes in the ocean environment over the past few decades. However, to date, artificial mass production of glass eels is far from reality because of the lack of appropriate feed for the eel larvae. In this study, wild glass eel, leptocephali, preleptocephali, and embryos were collected to conduct RNA-seq. Approximately 279 million reads were generated and assembled into 224,043 transcripts. The transcript levels of genes coding for digestive enzymes and nutrient transporters were investigated to estimate the capacities for nutrient digestion and absorption during early development. The results showed that the transcript levels of protein digestion enzymes were higher than those of carbohydrate and lipid digestion enzymes in the preleptocephali and leptocephali, and the transcript levels of amino acid transporters were also higher than those of glucose and fructose transporters and the cholesterol transporter. In addition, the transcript levels of glucose and fructose transporters were significantly raising in the leptocephali. Moreover, the transcript levels of protein, carbohydrate, and lipid digestion enzymes were balanced in the glass eel, but the transcript levels of amino acid transporters were higher than those of glucose and cholesterol transporters. These findings implied that preleptocephali and leptocephali prefer high-protein food, and the nutritional requirements of monosaccharides and lipids for the eel larvae vary with growth. An online database (http://molas.iis.sinica.edu.tw/jpeel/) that will provide the sequences and the annotated results of assembled transcripts was established for the eel research community.
De Novo Assembly of the Whole Transcriptome of the Wild Embryo, Preleptocephalus, Leptocephalus, and Glass Eel of Anguilla japonica and Deciphering the Digestive and Absorptive Capacities during Early Development

PubMed Central

Cha, Yuh-Ru; Tsukamoto, Katsumi; Lin, Chung-Yen; Han, Yu-San

2015-01-01

Natural stocks of Japanese eel (Anguilla japonica) have decreased drastically because of overfishing, habitat destruction, and changes in the ocean environment over the past few decades. However, to date, artificial mass production of glass eels is far from reality because of the lack of appropriate feed for the eel larvae. In this study, wild glass eel, leptocephali, preleptocephali, and embryos were collected to conduct RNA-seq. Approximately 279 million reads were generated and assembled into 224,043 transcripts. The transcript levels of genes coding for digestive enzymes and nutrient transporters were investigated to estimate the capacities for nutrient digestion and absorption during early development. The results showed that the transcript levels of protein digestion enzymes were higher than those of carbohydrate and lipid digestion enzymes in the preleptocephali and leptocephali, and the transcript levels of amino acid transporters were also higher than those of glucose and fructose transporters and the cholesterol transporter. In addition, the transcript levels of glucose and fructose transporters were significantly raising in the leptocephali. Moreover, the transcript levels of protein, carbohydrate, and lipid digestion enzymes were balanced in the glass eel, but the transcript levels of amino acid transporters were higher than those of glucose and cholesterol transporters. These findings implied that preleptocephali and leptocephali prefer high-protein food, and the nutritional requirements of monosaccharides and lipids for the eel larvae vary with growth. An online database (http://molas.iis.sinica.edu.tw/jpeel/) that will provide the sequences and the annotated results of assembled transcripts was established for the eel research community. PMID:26406914
Improved pedestrian safety at signalized intersections operating the flashing yellow arrow.

DOT National Transportation Integrated Search

2013-04-01

In some jurisdictions, protected left-turn phasing has been replaced with the flashing yellow arrow (FYA) for : protected/permissive left turns (PPLTs) to reduce delay. However, it is important to have a thorough understanding of the : conflict betwe...
Genetic diversity in a morphologically conservative invasive taxon: Multiple introductions of swamp eels to the southeastern United States

USGS Publications Warehouse

Collins, T.M.; Trexler, J.C.; Nico, L.G.; Rawlings, T.A.

2002-01-01

Genetic analysis of introduced populations, especially in morphologically conservative taxa, can clarify introduction histories, identify management units and source populations, provide a more realistic estimate of the frequency of successful invasion, and suggest strategies for preventing further introductions. In the last 7 years, populations of swamp eels, referred to the Asian genus Monopterus (Family Synbranchidae) on the basis of external morphology, have been discovered in aquatic habitats near Atlanta, Georgia; Tampa, Florida; North Miami, Florida; and most recently in close proximity to Everglades National Park in Homestead, Florida. Swamp eels are large predators capable of dispersal over land and have the potential to disrupt already threatened ecosystems. We analyzed mitochondrial DNA sequences from four known populations in the continental United States and samples from Malaysia, Indonesia, Vietnam, and two locations in China to determine introduction histories, source populations, genetic diversity, and relationships among populations. Our results indicate that there have been at least three independent introductions of genetically distinct forms. Introduced populations in close proximity (separated by <40 km) are genetically distinct. The level of sequence difference among introduced populations reaches levels seen among sister families of teleost fishes for the same region of the mitochondrial genome. These genetically distinct introduced populations in all likelihood represent at least two and possibly three species. Regardless of species status, these genetically distinct lineages may be expected to vary in ecological or life-history traits, representing different potential threats to the ecosystems where they have been introduced. Given the success of swamp eels in invading many habitats around the world, further study of these eels is warranted to elucidate the characteristics of successful invaders and invasions.
The yellow x paper birch hybrid--a potential substitute for yellow birch on problem sites

Treesearch

Knud E. Clausen

1977-01-01

Yellow x paper birch hybrids and yellow birches with common female parents were compared after 5 growing seasons in an open field. Survival of the hybrids was 91 percent compared with 64 percent for the yellow birch trees. The hybrids were from 25 to 32 percent taller than the yellow birches and had 19-40 percent greater diameter. Because this hybrid not only grows...
Laboratory Jet Erosion Tests on the Lower American River Soil Samples, Sacramento, CA- Phase 2

DTIC Science & Technology

2017-05-01

ER D C/ G SL T R- 17 -8 Laboratory Jet Erosion Tests on the Lower American River Soil Samples, Sacramento, CA – Phase 2 G eo te ch ni...Jet Erosion Tests on the Lower American River Soil Samples, Sacramento, CA – Phase 2 Johannes L. Wibowo and Bryant A. Robbins Geotechnical and...Appendix B: Soil Mechanics Data ........................................................................................................... 71
Geographic patterns and environmental factors associated with human yellow fever presence in the Americas

PubMed Central

Aldighieri, Sylvain; Machado, Gustavo; Leonel, Deise Galan; Vilca, Luz Maria; Uriona, Sonia; Schneider, Maria Cristina

2017-01-01

Background In the Americas, yellow fever virus transmission is a latent threat due to the proximity between urban and wild environments. Although yellow fever has nearly vanished from North and Central America, there are still 13 countries in the Americas considered endemic by the World Health Organization. Human cases usually occur as a result of the exposure to sylvatic yellow fever in tropical forested environments; but urban outbreaks reported during the last decade demonstrate that the risk in this environment still exists. The objective of this study was to identify spatial patterns and the relationship between key geographic and environmental factors with the distribution of yellow fever human cases in the Americas. Methodology/Principal findings An ecological study was carried out to analyze yellow fever human cases reported to the Pan American Health Organization from 2000 to 2014, aggregated by second administrative level subdivisions (counties). Presence of yellow fever by county was used as the outcome variable and eight geo-environmental factors were used as independent variables. Spatial analysis was performed to identify and examine natural settings per county. Subsequently, a multivariable logistic regression model was built. During the study period, 1,164 cases were reported in eight out of the 13 endemic countries. Nearly 83.8% of these cases were concentrated in three countries: Peru (37.4%), Brazil (28.1%) and Colombia (18.4%); and distributed in 57 states/provinces, specifically in 286 counties (3.4% of total counties). Yellow fever presence was significantly associated with altitude, rain, diversity of non-human primate hosts and temperature. A positive spatial autocorrelation revealed a clustered geographic pattern in 138/286 yellow fever positive counties (48.3%). Conclusions/Significance A clustered geographic pattern of yellow fever was identified mostly along the Andes eastern foothills. This risk map could support health policies in

Geographic patterns and environmental factors associated with human yellow fever presence in the Americas.

PubMed

Hamrick, Patricia Najera; Aldighieri, Sylvain; Machado, Gustavo; Leonel, Deise Galan; Vilca, Luz Maria; Uriona, Sonia; Schneider, Maria Cristina

2017-09-01

In the Americas, yellow fever virus transmission is a latent threat due to the proximity between urban and wild environments. Although yellow fever has nearly vanished from North and Central America, there are still 13 countries in the Americas considered endemic by the World Health Organization. Human cases usually occur as a result of the exposure to sylvatic yellow fever in tropical forested environments; but urban outbreaks reported during the last decade demonstrate that the risk in this environment still exists. The objective of this study was to identify spatial patterns and the relationship between key geographic and environmental factors with the distribution of yellow fever human cases in the Americas. An ecological study was carried out to analyze yellow fever human cases reported to the Pan American Health Organization from 2000 to 2014, aggregated by second administrative level subdivisions (counties). Presence of yellow fever by county was used as the outcome variable and eight geo-environmental factors were used as independent variables. Spatial analysis was performed to identify and examine natural settings per county. Subsequently, a multivariable logistic regression model was built. During the study period, 1,164 cases were reported in eight out of the 13 endemic countries. Nearly 83.8% of these cases were concentrated in three countries: Peru (37.4%), Brazil (28.1%) and Colombia (18.4%); and distributed in 57 states/provinces, specifically in 286 counties (3.4% of total counties). Yellow fever presence was significantly associated with altitude, rain, diversity of non-human primate hosts and temperature. A positive spatial autocorrelation revealed a clustered geographic pattern in 138/286 yellow fever positive counties (48.3%). A clustered geographic pattern of yellow fever was identified mostly along the Andes eastern foothills. This risk map could support health policies in endemic countries. Geo-environmental factors associated with presence
Flesh color inheritance and gene interactions among canary yellow, pale yellow and red watermelon

USDA-ARS?s Scientific Manuscript database

Two loci, C and i-C were previously reported to determine flesh color between canary yellow and red watermelon. Recently LCYB was found as a color determinant gene for canary yellow (C) and co-dominant CAPS marker was developed to identify canary yellow and red alleles. Another report suggested th...
Evidence for High Concentrations and Maternal Transfer of Substituted Diphenylamines in European Eels Analyzed by Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry and Gas Chromatography-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.

PubMed

Sühring, Roxana; Ortiz, Xavier; Pena-Abaurrea, Miren; Jobst, Karl J; Freese, Marko; Pohlmann, Jan-Dag; Marohn, Lasse; Ebinghaus, Ralf; Backus, Sean; Hanel, Reinhold; Reiner, Eric J

2016-12-06

Chemical pollution is hypothesized to be one of the factors driving the strong decline of the critically endangered European eel population. Specifically, the impact of contaminants on the quality of spawning eels and subsequent embryo survival and development has been discussed as crucial investigation point. However, so far, only very limited information on potential negative effects of contaminants on the reproduction of eels is available. Through the combination of nontargeted ultrahigh-resolution mass spectrometry and multidimensional gas chromatography, combined with more-conventional targeted analytical approaches and multimedia mass-balance modeling, compounds of particular relevance, and their maternal transfer in artificially matured European eels from the German river Ems have been identified. Substituted diphenylamines were, unexpectedly, found to be the primary organic contaminants in the eel samples, with concentrations in the μg g -1 wet weight range. Furthermore, it could be shown that these contaminants, as well as polychlorinated biphenyls (PCBs), organochlorine pesticides, and polyaromatic hydrocarbons (PAHs), are not merely stored in lipid rich tissue of eels but maternally transferred into gonads and eggs. The results of this study provide unique information on both the fate and behavior of substituted diphenylamines in the environment as well as their relevance as contaminants in European eels.
Beet yellow stunt

USDA-ARS?s Scientific Manuscript database

Beet yellow stunt virus (BYSV) is a potentially destructive yellows-type virus affecting plants in the family Asteraceae. The virus is a member of the genus Closterovirus, family Closteroviridae, and has been found in California and England. Initial symptoms consist of chlorosis of the older leaves,...
The effect of earthworms (Lumbricus rubellus) in feed formulation on growth and retention of eel (Anguilla bicolor)

NASA Astrophysics Data System (ADS)

Jatmiko, P. C.; Madinah, N. A.; Agustono; Nurhajati, T.

2018-04-01

Earthworms (Lumbricus rubellus) has high protein content. The addition of earthworms in formulation feed not only can increase the appetite of eel but also increase the nutritional content in feed. The purpose of this research was to know the potention of earthworms L. rubellus in feed formulation that can gives increase on the growth and retention. Research’s method was using Complete Randomized Design (CRD) consisted of five treatments and four replication. Treatments in this research ware the different addition of earthworms L. rubellus in feed formulation which were 0 %, 25 %, 50 %, 75 % and 100 %. The result showed that there were significantly different on the growth and retention of eel during maintenance for 21 days. the best result was on the 100% of earthworms L.rubellus addition.
Some benefits, problems, and concerns of a secondary user of yellow birch

Treesearch

John E. Bent

1969-01-01

S. Bent & Brothers, Inc. is a small furniture company located in Gardner, Massachusetts, specializing in the manufacture of Early American wooden seat chairs. Because of our size and our specialization, we do not necessarily represent the industry in our ideas about and use of yellow birch as a primary raw material. Our company during the past 100 years has made...
Genetic Relatedness among Environmental, Clinical, and Diseased-Eel Vibrio vulnificus Isolates from Different Geographic Regions by Ribotyping and Randomly Amplified Polymorphic DNA PCR

PubMed Central

Arias, Covadonga R.; Pujalte, María Jesús; Garay, Esperanza; Aznar, Rosa

1998-01-01

Genetic relationships among 132 strains of Vibrio vulnificus (clinical, environmental, and diseased-eel isolates from different geographic origins, as well as seawater and shellfish isolates from the western Mediterranean coast, including reference strains) were analyzed by random amplified polymorphic DNA (RAPD) PCR. Results were validated by ribotyping. For ribotyping, DNAs were digested with KpnI and hybridized with an oligonucleotide probe complementary to a highly conserved sequence in the 23S rRNA gene. Random amplification of DNA was performed with M13 and T3 universal primers. The comparison between ribotyping and RAPD PCR revealed an overall agreement regarding the high level of homogeneity of diseased-eel isolates in contrast to the genetic heterogeneity of Mediterranean isolates. The latter suggests the existence of autochthonous clones present in Mediterranean coastal waters. Both techniques have revealed a genetic proximity among Spanish fish farm isolates and a close relationship between four Spanish eel farm isolates and some Mediterranean isolates. Whereas the differentiation within diseased-eel isolates was only possible by ribotyping, RAPD PCR was able to differentiate phenotypically atypical isolates of V. vulnificus. On the basis of our results, RAPD PCR is proposed as a better technique than ribotyping for rapid typing in the routine analysis of new V. vulnificus isolates. PMID:9726889
A ddRAD-based genetic map and its integration with the genome assembly of Japanese eel (Anguilla japonica) provides insights into genome evolution after the teleost-specific genome duplication

PubMed Central

2014-01-01

Background Recent advancements in next-generation sequencing technology have enabled cost-effective sequencing of whole or partial genomes, permitting the discovery and characterization of molecular polymorphisms. Double-digest restriction-site associated DNA sequencing (ddRAD-seq) is a powerful and inexpensive approach to developing numerous single nucleotide polymorphism (SNP) markers and constructing a high-density genetic map. To enrich genomic resources for Japanese eel (Anguilla japonica), we constructed a ddRAD-based genetic map using an Ion Torrent Personal Genome Machine and anchored scaffolds of the current genome assembly to 19 linkage groups of the Japanese eel. Furthermore, we compared the Japanese eel genome with genomes of model fishes to infer the history of genome evolution after the teleost-specific genome duplication. Results We generated the ddRAD-based linkage map of the Japanese eel, where the maps for female and male spanned 1748.8 cM and 1294.5 cM, respectively, and were arranged into 19 linkage groups. A total of 2,672 SNP markers and 115 Simple Sequence Repeat markers provide anchor points to 1,252 scaffolds covering 151 Mb (13%) of the current genome assembly of the Japanese eel. Comparisons among the Japanese eel, medaka, zebrafish and spotted gar genomes showed highly conserved synteny among teleosts and revealed part of the eight major chromosomal rearrangement events that occurred soon after the teleost-specific genome duplication. Conclusions The ddRAD-seq approach combined with the Ion Torrent Personal Genome Machine sequencing allowed us to conduct efficient and flexible SNP genotyping. The integration of the genetic map and the assembled sequence provides a valuable resource for fine mapping and positional cloning of quantitative trait loci associated with economically important traits and for investigating comparative genomics of the Japanese eel. PMID:24669946
A ddRAD-based genetic map and its integration with the genome assembly of Japanese eel (Anguilla japonica) provides insights into genome evolution after the teleost-specific genome duplication.

PubMed

Kai, Wataru; Nomura, Kazuharu; Fujiwara, Atushi; Nakamura, Yoji; Yasuike, Motoshige; Ojima, Nobuhiko; Masaoka, Tetsuji; Ozaki, Akiyuki; Kazeto, Yukinori; Gen, Koichiro; Nagao, Jiro; Tanaka, Hideki; Kobayashi, Takanori; Ototake, Mitsuru

2014-03-26

Recent advancements in next-generation sequencing technology have enabled cost-effective sequencing of whole or partial genomes, permitting the discovery and characterization of molecular polymorphisms. Double-digest restriction-site associated DNA sequencing (ddRAD-seq) is a powerful and inexpensive approach to developing numerous single nucleotide polymorphism (SNP) markers and constructing a high-density genetic map. To enrich genomic resources for Japanese eel (Anguilla japonica), we constructed a ddRAD-based genetic map using an Ion Torrent Personal Genome Machine and anchored scaffolds of the current genome assembly to 19 linkage groups of the Japanese eel. Furthermore, we compared the Japanese eel genome with genomes of model fishes to infer the history of genome evolution after the teleost-specific genome duplication. We generated the ddRAD-based linkage map of the Japanese eel, where the maps for female and male spanned 1748.8 cM and 1294.5 cM, respectively, and were arranged into 19 linkage groups. A total of 2,672 SNP markers and 115 Simple Sequence Repeat markers provide anchor points to 1,252 scaffolds covering 151 Mb (13%) of the current genome assembly of the Japanese eel. Comparisons among the Japanese eel, medaka, zebrafish and spotted gar genomes showed highly conserved synteny among teleosts and revealed part of the eight major chromosomal rearrangement events that occurred soon after the teleost-specific genome duplication. The ddRAD-seq approach combined with the Ion Torrent Personal Genome Machine sequencing allowed us to conduct efficient and flexible SNP genotyping. The integration of the genetic map and the assembled sequence provides a valuable resource for fine mapping and positional cloning of quantitative trait loci associated with economically important traits and for investigating comparative genomics of the Japanese eel.
Flood inundation maps for the Wabash and Eel Rivers at Logansport, Indiana

USGS Publications Warehouse

Fowler, Kathleen K.

2014-01-01

Digital flood-inundation maps for an 8.3-mile reach of the Wabash River and a 7.6-mile reach of the Eel River at Logansport, Indiana (Ind.), were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Office of Community and Rural Affairs. The inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science Web site at http://water.usgs.gov/osw/flood_inundation/, depict estimates of the areal extent and depth of flooding corresponding to selected water levels (stages) at USGS streamgage Wabash River at Logansport, Ind. (sta. no. 03329000) and USGS streamgage Eel River near Logansport, Ind. (sta. no. 03328500). Current conditions for estimating near-real-time areas of inundation using USGS streamgage information may be obtained on the Internet at http://waterdata.usgs.gov/. In addition, information has been provided to the National Weather Service (NWS) for incorporation into their Advanced Hydrologic Prediction Service (AHPS) flood warning system http:/water.weather.gov/ahps/). The NWS forecasts flood hydrographs at many places that are often colocated with USGS streamgages. NWS-forecasted peak-stage information may be used in conjunction with the maps developed in this study to show predicted areas of flood inundation. For this study, flood profiles were computed for the stream reaches by means of a one-dimensional step-backwater model developed by the U.S. Army Corps of Engineers. The hydraulic model was calibrated by using the most current stage-discharge relations at USGS streamgages 03329000, Wabash River at Logansport, Ind., and 03328500, Eel River near Logansport, Ind. The calibrated hydraulic model was then used to determine five water-surface profiles for flood stage at 1-foot intervals referenced to the Wabash River streamgage datum, and four water-surface profiles for flood stages at 1-foot intervals referenced to the Eel River streamgage datum. The stages range from bankfull to approximately the highest
Wide-Scope Screening Method for Multiclass Veterinary Drug Residues in Fish, Shrimp, and Eel Using Liquid Chromatography-Quadrupole High-Resolution Mass Spectrometry.

PubMed

Turnipseed, Sherri B; Storey, Joseph M; Lohne, Jack J; Andersen, Wendy C; Burger, Robert; Johnson, Aaron S; Madson, Mark R

2017-08-30

A screening method for veterinary drug residues in fish, shrimp, and eel using LC with a high-resolution MS instrument has been developed and validated. The method was optimized for over 70 test compounds representing a variety of veterinary drug classes. Tissues were extracted by vortex mixing with acetonitrile acidified with 2% acetic acid and 0.2% p-toluenesulfonic acid. A centrifuged portion of the extract was passed through a novel solid phase extraction cartridge designed to remove interfering matrix components from tissue extracts. The eluent was then evaporated and reconstituted for analysis. Data were collected with a quadrupole-Orbitrap high-resolution mass spectrometer using both nontargeted and targeted acquisition methods. Residues were detected on the basis of the exact mass of the precursor and a product ion along with isotope pattern and retention time matching. Semiquantitative data analysis compared MS 1 signal to a one-point extracted matrix standard at a target testing level. The test compounds were detected and identified in salmon, tilapia, catfish, shrimp, and eel extracts fortified at the target testing levels. Fish dosed with selected analytes and aquaculture samples previously found to contain residues were also analyzed. The screening method can be expanded to monitor for an additional >260 veterinary drugs on the basis of exact mass measurements and retention times.
Elm yellows: a widespread and overlooked killer of elm trees across the United States

Treesearch

Charles E. Flower; Nancy Hayes-Plazolles; Cristina Rosa; James M. Slavicek

2017-01-01

The elm yellows phytoplasma (Candidatus Phytoplasma ulmi) is a geographically widespread pathogen that poses a significant threat to most native wild elms in North America (Ulmus americana, U. rubra, U. alata, U. crassifolia, and U. serotina) (MÃ¤urer et al. 1993), as well as to the success of American elm...
Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster.

PubMed

Tsutsui, Shigeyuki; Yoshino, Yuko; Matsui, Saho; Nakamura, Osamu; Muramoto, Koji; Watanabe, Tasuku

2008-03-01

By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.
Benchmarking the stability of human detergent-solubilised voltage-gated sodium channels for structural studies using eel as a reference

PubMed Central

Slowik, Daria; Henderson, Richard

2015-01-01

With the ultimate goal of detailed structural analysis of mammalian and particularly human voltage-gated sodium channels (VGSCs), we have investigated the relative stability of human and rat VGSCs and compared them with electric eel VGSC. We found that NaV1.3 from rat was the most stable after detergent solubilisation. The order of stability was rNaV1.3 > hNaV1.2 > hNaV1.1 > hNaV1.6 > hNaV1.3 > hNaV1.4. However, a comparison with the VGSC from Electrophorus electricus, which is most similar to NaV1.4, shows that the eel VGSC is considerably more stable in detergent than the human VGSCs examined. We conclude that current methods of structural analysis, such as single particle electron cryomicroscopy (cryoEM), may be most usefully targeted to eel VGSC or rNaV1.3, but that structural analysis on the full spectrum of VGSCs, by methods that require greater stability such as crystallisation and X-ray crystallography, will require further stabilisation of the channel. PMID:25838126
A note on the correlation between rainfall and the prevalence of Gnathostoma spp. infective stage larvae in swamp eels in Thailand.

PubMed

Wiwanitkit, V

2004-12-01

Gnathostoma spinigerum is the major causative agent of human gnathostomiasis, a parasitic zoonosis with a great variety of clinical manifestations. Generally, humans are infected by consumption of third-stage larvae (L3) of G. spinigerum in infected hosts in the form of partially cooked or uncooked food. Surveys of the contamination of Gnathostoma spp. L3 in swamp eels are useful for prevention and control of diseases and have been continuously performed in Thailand. The author performed a retrospective study on 33 previous cross-sectional surveys with geographical data and the prevalence of Gnathostoma spp. L3 that covered 12 provinces in Thailand. The relation between rainfall (derived from the geographical data) and the prevalence of Gnathostomo spp. L3 in swamp eels (derived from the overall infection rate of Gnathostoma spp. L3) was investigated. The least-square equation plot rainfall (y) versus prevalence (x) is y= 9.68x + 1,035.12 (r = 0.83; p < 0.01). A significant correlation was discerned between rainfall and the prevalence of eel infection but not for the season of the survey. Similar to the previous study, the prevalence of eel infection may depend on rainfall rather than season. However, this study focused on only 33 cross-sectional surveys in Thailand; further similar study in other countries to assess the correlation between rainfall and the prevalence of infection is required to substantiate this conclusion.
Crotoxin and phospholipases A₂ from Crotalus durissus terrificus showed antiviral activity against dengue and yellow fever viruses.

PubMed

Muller, Vanessa Danielle Menjon; Russo, Raquel Rinaldi; Cintra, Adelia Cristina Oliveira; Sartim, Marco Aurélio; Alves-Paiva, Raquel De Melo; Figueiredo, Luiz Tadeu Moraes; Sampaio, Suely Vilela; Aquino, Victor Hugo

2012-03-15

Dengue is the most important arbovirus in the world with an estimated of 50 million dengue infections occurring annually and approximately 2.5 billion people living in dengue endemic countries. Yellow fever is a viral hemorrhagic fever with high mortality that is transmitted by mosquitoes. Effective vaccines against yellow fever have been available for almost 70 years and are responsible for a significant reduction of occurrences of the disease worldwide; however, approximately 200,000 cases of yellow fever still occur annually, principally in Africa. Therefore, it is a public health priority to develop antiviral agents for treatment of these virus infections. Crotalus durissus terrificus snake, a South American rattlesnake, presents venom with several biologically actives molecules. In this study, we evaluated the antiviral activity of crude venom and isolated toxins from Crotalus durissus terrificus and found that phospholipases A₂ showed a high inhibition of Yellow fever and dengue viruses in VERO E6 cells. Copyright Â© 2011 Elsevier Ltd. All rights reserved.
Fipronil and two of its transformation products in water and European eel from the river Elbe.

PubMed

Michel, N; Freese, M; Brinkmann, M; Pohlmann, J-D; Hollert, H; Kammann, U; Haarich, M; Theobald, N; Gerwinski, W; Rotard, W; Hanel, R

2016-10-15

Fipronil is an insecticide which, based on its mode of action, is intended to be predominantly toxic towards insects. Fipronil bioaccumulates and some of its transformation products were reported to be similar or even more stable in the environment and to show an enhanced toxicity against non-target organisms compared to the parent compound. The current study investigated the occurrence of Fipronil and two of its transformation products, Fipronil-desulfinyl and Fipronil-sulfone, in water as well as muscle and liver samples of eels from the river Elbe (Germany). In water samples total concentrations of FIP, FIP-d and FIP-s ranged between 0.5-1.6ngL(-1) with FIP being the main component in all water samples followed by FIP-s and FIP-d. In contrast, FIP-s was the main component in muscle and liver tissues of eels with concentrations of 4.05±3.73ngg(-1) ww and 19.91±9.96ngg(-1) ww, respectively. Using a physiologically based toxicokinetic (PBTK) model for moderately hydrophobic organic chemicals, the different distributions of FIP, FIP-d and FIP-s in water and related tissue samples could be attributed to metabolic processes of eels. The measured concentrations in water of all analytes and their fractional distribution did not reflect the assumed seasonal application of FIP and it seems that the water was constantly contaminated with FIP, FIP-d and FIP-s. Copyright © 2016 Elsevier B.V. All rights reserved.
Insect enemies of yellow-poplar

Treesearch

Denver P. Burns; Denver P. Burns

1970-01-01

Yellow-poplar, like the other desirable hardwoods, is attacked by a variety of insects. However, only four species of insects are considered economically important: the tuliptree scale, the yellow-poplar weevil, the root-collar borer, and the Columbian timber beetle. These are native enemies of yellow-poplar (Liriodendvon tzllipifera L.) wherever the tree grows.
Evidence for population bottlenecks and subtle genetic structure in the yellow rail

USGS Publications Warehouse

Popper, Kenneth J.; Miller, Leonard F.; Green, Michael; Haig, Susan M.; Mullins, Thomas D.

2012-01-01

The Yellow Rail (Coturnicops noveboracencis) is among the most enigmatic and least studied North American birds. Nesting exclusively in marshes and wetlands, it breeds largely east of the Rocky Mountains in the northern United States and Canada, but there is an isolated population in southern Oregon once believed extirpated. The degree of connectivity of the Oregon population with the main population is unknown. We used mitochondrial DNA sequences (mtDNA) and six microsatellite loci to characterize the Yellow Rail's genetic structure and diversity patterns in six areas. Our mtDNA-based analyses of genetic structure identified significant population differentiation, but pairwise comparison of regions identified no clear geographic trends. In contrast, microsatellites suggested subtle genetic structure differentiating the Oregon population from those in the five regions sampled in the Yellow Rail's main breeding range. The genetic diversity of the Oregon population was also the lowest of the six regions sampled, and Oregon was one of three regions that demonstrated evidence of recent population bottlenecks. Factors that produced population reductions may include loss of wetlands to development and agricultural conversion, drought, and wildfire. At this time, we are unable to determine if the high percentage (50%) of populations having experienced bottlenecks is representative of the Yellow Rail's entire range. Further genetic data from additional breeding populations will be required for this issue to be addressed.
Sedimentary Carbon, Sulfur, and Iron Relationships in Modern and Ancient Diagenetic Environments of the Eel River Basin (U.S.A.)

USGS Publications Warehouse

Sommerfield, C.K.; Aller, R.C.; Nittrouer, C.A.

2001-01-01

Depositional and diagenetic controls on the distributions of carbon, sulfur, and iron (C-S-Fe) in modern sediments and upper Pleistocene mudrocks of the Eel River Basin (ERB), northern California continental margin, were investigated using a combination of geochemical, radioisotopic, and sedimentological methods. A mass balance based on down-core profiles of porewater and solid-phase constituents and diagenetic modeling suggests that only 12-30% of the pyrite-S produced via SO4-2 reduction during burial is retained in modern shelf and upper slope deposits of the ERB. Bioturbational reoxidation of initially reduced S is inferred to be the major control on S preservation, on the basis of an observed inverse relationship between pyrite-S retention and biological mixing intensity, estimated from profiles of excess 234Th. Importantly, these findings argue that massive depositional episodes on the shelf following floods of the Eel River have a negligible long-term impact on bioturbating macrofauna and the potential to affect geochemical properties of the sediments. Down-core profiles of reactive Fe3+and Py-Fe(II) for the modern deposits suggest that highly reactive Fe phases are sulfidized well within ∼ 500-2000 years of burial, thereby limiting later pyritization, which could occur through sulfidation of less reactive phases. This result explains the low (≤ 0.4) degree of pyritization (DOP) values exhibited by both modern and ancient deposits of the ERB and lends support to the notion that pyritization in aerobic continental-margin sediments is largely associated with highly reactive detrital Fe oxides. Comparable mean C/S weight ratios for modern sediments (5.4 ± 3.3, 1σ) and mudrocks (6.9 ± 4.5) of the ERB suggest that the upper Pleistocene strata reflect a geochemical environment analogous to that of the modern margin. Specifically, the C-S-Fe signatures shared by the modern and ancient deposits are a consequence of similar detrital Fe mineralogies, initial

A multi-locus molecular timescale for the origin and diversification of eels (Order: Anguilliformes).

PubMed

Santini, Francesco; Kong, Xianghui; Sorenson, Laurie; Carnevale, Giorgio; Mehta, Rita S; Alfaro, Michael E

2013-12-01

Anguilliformes are an ecologically diverse group of predominantly marine fishes whose members are easily recognized by their extremely elongate bodies, and universal lack of pelvic fins. Recent studies based on mitochondrial loci, including full mitogenomes, have called into question the monophyly of both the Anguilliformes, which appear to be paraphyletic without the inclusion of the Saccopharyngiformes (gulper eels and allies), as well as other more commonly known eel families (e.g., Congridae, Serrivomeridae). However, no study to date has investigated anguilliform interrelationships using nuclear loci. Here we present a new phylogenetic hypothesis for the Anguilliformes based on five markers (the nuclear loci Early Growth Hormone 3, Myosin Heavy Polypeptide 6 and Recombinase Activating Gene 1, as well as the mitochondrial genes Cytochrome b and Cytochrome Oxidase I). Our sampling spans 148 species and includes 19 of the 20 extant families of anguilliforms and saccopharyngiforms. Maximum likelihood analysis reveals that saccopharyngiform eels are deeply nested within the anguilliforms, and supports the non-monophyly of Congridae and Nettastomatidae, as well as that of Derichthyidae and Chlopsidae. Our analyses suggest that Protanguilla may be the sister group of the Synaphobranchidae, though the recent hypothesis that this species is the sister group to all other anguilliforms cannot be rejected. The molecular phylogeny, time-calibrated using a Bayesian relaxed clock approach and seven fossil calibration points, reveals a Late Cretaceous origin of this expanded anguilliform clade (stem age ~116 Ma, crown age ~99 Ma). Most major (family level) lineages originated between the end of the Cretaceous and Early Eocene, suggesting that anguilliform radiation may have been facilitated by the recovery of marine ecosystems following the KP extinction. Copyright © 2013 Elsevier Inc. All rights reserved.
Identification of gonadal soma-derived factor involvement in Monopterus albus (protogynous rice field eel) sex change.

PubMed

Zhu, Yefei; Wang, Chunlei; Chen, Xiaowu; Guan, Guijun

2016-07-01

We studied molecular events and potential mechanisms underlying the process of female-to-male sex transformation in the rice field eel (Monopterus albus), a protogynous hermaphrodite fish in which the gonad is initially a female ovary and transforms into male testes. We cloned and identified a novel gonadal soma derived factor (GSDF), which encodes a member of the transforming growth factor-beta superfamily. gsdf expression was measured in gonads of female, intersex and male with reverse transcription-PCR and gsdf's role in sex transformation was studied with qPCR, histological analysis and dual-color in situ hybridization assays and compared to other sex-related genes. gsdf was correlated to Sertoli cell differentiation, indicating involvement in testicular differentiation and sex transformation from female to male in this species. A unique expression pattern reveals a potential role of gsdf essential for the sex transformation of rice field eels.
Salinity reduction benefits European eel larvae: Insights at the morphological and molecular level.

PubMed

Politis, Sebastian N; Mazurais, David; Servili, Arianna; Zambonino-Infante, Jose-Luis; Miest, Joanna J; Tomkiewicz, Jonna; Butts, Ian A E

2018-01-01

European eel (Anguilla anguilla) is a euryhaline species, that has adapted to cope with both, hyper- and hypo-osmotic environments. This study investigates the effect of salinity, from a morphological and molecular point of view on European eel larvae reared from 0 to 12 days post hatch (dph). Offspring reared in 36 practical salinity units (psu; control), were compared with larvae reared in six scenarios, where salinity was decreased on 0 or 3 dph and in rates of 1, 2 or 4 psu/day, towards iso-osmotic conditions. Results showed that several genes relating to osmoregulation (nkcc2α, nkcc2β, aqp1dup, aqpe), stress response (hsp70, hsp90), and thyroid metabolism (thrαA, thrαB, thrβB, dio1, dio2, dio3) were differentially expressed throughout larval development, while nkcc1α, nkcc2β, aqp3, aqp1dup, aqpe, hsp90, thrαA and dio3 showed lower expression in response to the salinity reduction. Moreover, larvae were able to keep energy metabolism related gene expression (atp6, cox1) at stable levels, irrespective of the salinity reduction. As such, when reducing salinity, an energy surplus associated to reduced osmoregulation demands and stress (lower nkcc, aqp and hsp expression), likely facilitated the observed increased survival, improved biometry and enhanced growth efficiency. Additionally, the salinity reduction decreased the amount of severe deformities such as spinal curvature and emaciation but also induced an edematous state of the larval heart, resulting in the most balanced mortality/deformity ratio when salinity was decreased on 3 dph and at 2 psu/day. However, the persistency of the pericardial edema and if or how it represents an obstacle in further larval development needs to be further clarified. In conclusion, this study clearly showed that salinity reduction regimes towards iso-osmotic conditions facilitated the European eel pre-leptocephalus development and revealed the existence of highly sensitive and regulated osmoregulation processes at such
Emerging brominated flame retardants and dechlorane-related compounds in European eels (Anguilla anguilla) from Latvian lakes.

PubMed

Zacs, D; Ikkere, L E; Bartkevics, V

2018-04-01

Fifteen halogenated flame retardants (HFRs) including seven emerging brominated flame retardants (EBFRs) and eight dechlorane-related compounds (DRCs) were analyzed in eels (Anguilla anguilla) sampled from five Latvian lakes. Out of the seven EBFRs, hexabromocyclododecane (HBCD) and decabromodiphenyl ethane (DBDPE) were found in eels in quantifiable concentrations, up to 6.58 and 33.0 ng g -1 lipid weight (l.w.), respectively. The mean total concentration of DRCs (∑ DRC ) in the samples was 0.62 ng g -1 l.w. and the geographical distribution of DRC contamination was nearly uniform among the selected lakes. Dechlorane 602 (Dec 602) was the predominant component, whereas the composition of mixture containing syn- and anti-Dechlorane Plus (DP) stereoisomers showed a pronounced enrichment of the anti-DP isomer and was close to the composition of OxyChem ® DP commercial product. The determined concentrations of HFRs were lower than in other studies of aquatic biota from Europe and Asia, and the obtained results reflect the acceptable environmental status of Latvian lakes with regard to the total content of HBCD (∑ HBCD ), considering the environmental quality standards (EQS) stated in the Directive 2013/39/EU. The highest ∑ HBCD levels were observed in eels from lakes corresponding to the industrialization of those areas, while the results of principal component analysis (PCA) showed that the concentration of HBCD depended on the particular sampling lake, reflecting non-uniform contamination of the Latvian environment with this EBFR. Copyright © 2018 Elsevier Ltd. All rights reserved.
Yellow fever: an update.

PubMed

Monath, T P

2001-08-01

Yellow fever, the original viral haemorrhagic fever, was one of the most feared lethal diseases before the development of an effective vaccine. Today the disease still affects as many as 200,000 persons annually in tropical regions of Africa and South America, and poses a significant hazard to unvaccinated travellers to these areas. Yellow fever is transmitted in a cycle involving monkeys and mosquitoes, but human beings can also serve as the viraemic host for mosquito infection. Recent increases in the density and distribution of the urban mosquito vector, Aedes aegypti, as well as the rise in air travel increase the risk of introduction and spread of yellow fever to North and Central America, the Caribbean and Asia. Here I review the clinical features of the disease, its pathogenesis and pathophysiology. The disease mechanisms are poorly understood and have not been the subject of modern clinical research. Since there is no specific treatment, and management of patients with the disease is extremely problematic, the emphasis is on preventative vaccination. As a zoonosis, yellow fever cannot be eradicated, but reduction of the human disease burden is achievable through routine childhood vaccination in endemic countries, with a low cost for the benefits obtained. The biological characteristics, safety, and efficacy of live attenuated, yellow fever 17D vaccine are reviewed. New applications of yellow fever 17D virus as a vector for foreign genes hold considerable promise as a means of developing new vaccines against other viruses, and possibly against cancers.
Phylogeny of Yellow Fever Virus, Uganda, 2016.

PubMed

Hughes, Holly R; Kayiwa, John; Mossel, Eric C; Lutwama, Julius; Staples, J Erin; Lambert, Amy J

2018-08-17

In April 2016, a yellow fever outbreak was detected in Uganda. Removal of contaminating ribosomal RNA in a clinical sample improved the sensitivity of next-generation sequencing. Molecular analyses determined the Uganda yellow fever outbreak was distinct from the concurrent yellow fever outbreak in Angola, improving our understanding of yellow fever epidemiology.
Yellow-poplar seedfall pattern

Treesearch

LaMont G. Engle

1960-01-01

Knowing the pattern of seedfall can be helpful when trying to regenerate yellow-poplar. This is especially true if the stand contains only scattered yellow-poplar seed trees. Information obtained from seed collections in Indiana shows that most of the seed falls north and northeast of seed trees.
21 CFR 137.215 - Yellow corn flour.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Yellow corn flour. 137.215 Section 137.215 Food... Flours and Related Products § 137.215 Yellow corn flour. Yellow corn flour conforms to the definition and standard of identity prescribed by § 137.211 for white corn flour except that cleaned yellow corn is used...
21 CFR 137.275 - Yellow corn meal.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Yellow corn meal. 137.275 Section 137.275 Food and... Related Products § 137.275 Yellow corn meal. Yellow corn meal conforms to the definition and standard of identity prescribed by § 137.250 for white corn meal except that cleaned yellow corn is used instead of...
21 CFR 137.215 - Yellow corn flour.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Yellow corn flour. 137.215 Section 137.215 Food... Flours and Related Products § 137.215 Yellow corn flour. Yellow corn flour conforms to the definition and standard of identity prescribed by § 137.211 for white corn flour except that cleaned yellow corn is used...
21 CFR 137.275 - Yellow corn meal.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Yellow corn meal. 137.275 Section 137.275 Food and... Related Products § 137.275 Yellow corn meal. Yellow corn meal conforms to the definition and standard of identity prescribed by § 137.250 for white corn meal except that cleaned yellow corn is used instead of...
Myo-inositol phosphate synthase expression in the European eel (Anguilla anguilla) and Nile tilapia (Oreochromis niloticus): effect of seawater acclimation

PubMed Central

Kalujnaia, Svetlana; Hazon, Neil

2016-01-01

A single MIPS gene (Isyna1/Ino1) exists in eel and tilapia genomes with a single myo-d-inositol 3-phosphate synthase (MIPS) transcript identified in all eel tissues, although two MIPS spliced variants [termed MIPS(s) and MIPS(l)] are found in all tilapia tissues. The larger tilapia transcript [MIPS(l)] results from the inclusion of the 87-nucleotide intron between exons 5 and 6 in the genomic sequence. In most tilapia tissues, the MIPS(s) transcript exhibits much higher abundance (generally >10-fold) with the exception of white skeletal muscle and oocytes, in which the MIPS(l) transcript predominates. SW acclimation resulted in large (6- to 32-fold) increases in mRNA expression for both MIPS(s) and MIPS(l) in all tilapia tissues tested, whereas in the eel, changes in expression were limited to a more modest 2.5-fold increase and only in the kidney. Western blots identified a number of species- and tissue-specific immunoreactive MIPS proteins ranging from 40 to 67 kDa molecular weight. SW acclimation failed to affect the abundance of any immunoreactive protein in any tissue tested from the eel. However, a major 67-kDa immunoreactive protein (presumed to be MIPS) found in tilapia tissues exhibited 11- and 54-fold increases in expression in gill and fin samples from SW-acclimated fish. Immunohistochemical investigations revealed specific immunoreactivity in the gill, fin, skin, and intestine taken from only SW-acclimated tilapia. Immunofluorescence indicated that MIPS was expressed within gill chondrocytes and epithelial cells of the primary filaments, basal epithelial cell layers of the skin and fin, the cytosol of columnar intestinal epithelial and mucous cells, as well as unknown entero-endocrine-like cells. PMID:27252471
Distribution of PAEs in the middle and lower reaches of the Yellow River, China.

PubMed

Sha, Yujuan; Xia, Xinghui; Yang, Zhifeng; Huang, Gordon H

2007-01-01

Samples of water, sediment and suspended particulates were collected from 13 sites in the middle and lower reaches of the Yellow River in China. Phthalic acid esters (PAEs) concentrations in different phases of each sample were determined by Gas Chromatogram GC-FID. The results are shown as follows: (1) In the Xiao Langdi-Dongming Bridge section, PAEs concentrations in water phase from the main river ranged from 3.99 x 10(-3) to 45.45 x 10(-3) mg/L, which were similar to those from other rivers in the world. The PAEs levels in the tributaries of the Yellow River were much higher than those of the main river. (2) In the studied branches, the concentration of PAEs in sediment for Luoyang Petrochemical Channel (331.70 mg/Kg) was the highest. The concentrations of PAEs in sediment phase of the main river were 30.52 to 85.16 mg/Kg, which were much higher than those from other rivers in the world. In the main river, the concentration level of PAEs on suspended solid phases reached 94.22 mg/Kg, and it reached 691.23 mg/Kg in the Yiluo River - one tributary of the Yellow River. (3) Whether in the sediment or on the suspended solid phases, there was no significant correlation between the contents of PAEs and TOC or particle size of the solid phase; and the calculated Koc of Di (2-Ethylhexyl) Phthalate (DEHP) in the river were much less than the theoretical value, which inferred that PAEs were not on the equilibrium between water and suspended solid phases/sediment. (4) Among the measured PAEs compounds, the proportions of DEHP and di-n-butyl phthalate (DBP) were much higher than the others. The concentrations of DEHP exceeded the Quality Standard in all the main river and tributary stations except those in the Mengjin and Jiaogong Bridge of the main river. This indicates that more attention should be paid to pollution control and further assessment in understanding risks associated with human health.
Exploring possibilities of band gap measurement with off-axis EELS in TEM.

PubMed

Korneychuk, Svetlana; Partoens, Bart; Guzzinati, Giulio; Ramaneti, Rajesh; Derluyn, Joff; Haenen, Ken; Verbeeck, Jo

2018-06-01

A technique to measure the band gap of dielectric materials with high refractive index by means of energy electron loss spectroscopy (EELS) is presented. The technique relies on the use of a circular (Bessel) aperture and suppresses Cherenkov losses and surface-guided light modes by enforcing a momentum transfer selection. The technique also strongly suppresses the elastic zero loss peak, making the acquisition, interpretation and signal to noise ratio of low loss spectra considerably better, especially for excitations in the first few eV of the EELS spectrum. Simulations of the low loss inelastic electron scattering probabilities demonstrate the beneficial influence of the Bessel aperture in this setup even for high accelerating voltages. The importance of selecting the optimal experimental convergence and collection angles is highlighted. The effect of the created off-axis acquisition conditions on the selection of the transitions from valence to conduction bands is discussed in detail on a simplified isotropic two band model. This opens the opportunity for deliberately selecting certain transitions by carefully tuning the microscope parameters. The suggested approach is experimentally demonstrated and provides good signal to noise ratio and interpretable band gap signals on reference samples of diamond, GaN and AlN while offering spatial resolution in the nm range. Copyright © 2018 Elsevier B.V. All rights reserved.
Collective modes in CuxTiSe2 measured with meV-resolution EELS

NASA Astrophysics Data System (ADS)

Rak, Melinda; Vig, Sean; Husain, Ali; Mitrano, Matteo; Rubeck, Samantha; Kogar, Anshul; Karapetrov, Goran; Morosan, Emilia; Abbamonte, Peter

The charge density wave (CDW) in 1 T-TiSe2 has been widely thought to be the result of an excitonic insulator transition. We recently observed a soft electronic mode in TiSe2 using a new, momentum-resolved electron energy loss spectroscopy (M-EELS) technique, demonstrating a condensation of electron-hole pairs in this material. As TiSe2 is doped with Cu to produce CuxTiSe2, a superconducting dome emerges above x ˜ 0.04. In this talk, I describe how the electronic collective mode evolves with Cu doping. We find that the temperature dependence of the electronic mode reverses as Cu is introduced and that the mode is much broader at low temperature as compared to the undoped material. Additionally, the electronic mode no longer has a positive dispersion at 300 K as described by the Lindhard function, but has a slightly negative dispersion for small momentum transfers. We will discuss the implications of these results for the excitonic insulator transition in TiSe2. This work was supported by the Gordon and Betty Moore Foundation's EPiQS Initiative through Grant GBMF4542. An early prototype of the M-EELS instrument was supported by the DOE Center for Emergent Superconductivity under Award No. DE-AC02-98CH10886.
Pre-steady-state charge translocation in NaK-ATPase from eel electric organ

PubMed Central

1993-01-01

Time-resolved measurements of charge translocation and phosphorylation kinetics during the pre-steady state of the NaK-ATPase reaction cycle are presented. NaK-ATPase-containing microsomes prepared from the electric organ of Electrophorus electricus were adsorbed to planar lipid bilayers for investigation of charge translocation, while rapid acid quenching was used to study the concomitant enzymatic partial reactions involved in phosphoenzyme formation. To facilitate comparison of these data, conditions were standardized with respect to pH (6.2), ionic composition, and temperature (24 degrees C). The different phases of the current generated by the enzyme are analyzed under various conditions and compared with the kinetics of phosphoenzyme formation. The slowest time constant (tau 3(-1) approximately 8 s-1) is related to the influence of the capacitive coupling of the adsorbed membrane fragments on the electrical signal. The relaxation time associated with the decaying phase of the electrical signal (tau 2(-1) = 10-70 s-1) depends on ATP and caged ATP concentration. It is assigned to the ATP and caged ATP binding and exchange reaction. A kinetic model is proposed that explains the behavior of the relaxation time at different ATP and caged ATP concentrations. Control measurements with the rapid mixing technique confirm this assignment. The rising phase of the electrical signal was analyzed with a kinetic model based on a condensed Albers-Post cycle. Together with kinetic information obtained from rapid mixing studies, the analysis suggests that electroneutral ATP release, ATP and caged ATP binding, and exchange and phosphorylation are followed by a fast electrogenic E1P-->E2P transition. At 24 degrees C and pH 6.2, the rate constant for the E1P-- >E2P transition in NaK-ATPase from eel electric organ is > or = 1,000 s- 1. PMID:8270908
Effects of the free fatty acid content in yellow grease on performance, carcass characteristics, and serum lipids in broilers.

PubMed

Wu, H; Gong, L M; Guo, L; Zhang, L Y; Li, J T

2011-09-01

This study was conducted to investigate whether the free fatty acid (FFA) content of yellow grease would influence the performance and carcass characteristics of broiler chicks. A total of 432 one-day-old, male Arbor Acres broilers were randomly allotted to 1 of 4 treatments, with each treatment being applied to 6 pens of 18 chicks. The dietary treatments were based on corn and soybean meal and were supplemented with either soybean oil (2.86% FFA) or yellow grease with a low (2.74%), medium (12.59%), or high (19.05%) FFA content. The fat sources were supplemented at 1.5% of the diet during the starter phase (0 to 21 d) and at 3.0% of the diet during the grower phase (22 to 42 d). From d 0 to 42, the BW gains of chicks fed diets containing soybean oil and yellow grease with 2.74% FFA were similar. As the FFA level in the yellow grease increased, the BW gain of chicks decreased (P < 0.01). The reduction in BW gain appeared to be mediated by a reduction in feed intake. The dressing percentage and the percentage of breast muscle in the carcass were significantly (P < 0.01) lower for broilers fed any yellow grease diet compared with birds fed soybean oil. In contrast, abdominal fat was significantly increased in diets containing yellow grease. These results demonstrate that the performance of birds fed yellow grease with a low content of FFA was essentially equal to that of birds fed soybean oil. However, because yellow grease samples containing FFA levels greater than 2.74% negatively affected bird performance, producers should exercise caution with regard to feeding broilers yellow grease with an elevated FFA content. In 42-d-old broilers, serum total cholesterol and low-density lipoprotein cholesterol levels were elevated in birds fed yellow grease, regardless of the dietary level. In contrast, serum high-density lipoprotein cholesterol and triglyceride levels were unaffected by dietary treatment. Although dietary FFA may influence triglyceride-rich lipoprotein
Japanese Americans, Pluralism, and the Model Minority Myth.

ERIC Educational Resources Information Center

Endo, George T.; Della-Piana, Connie Kubo

1981-01-01

The current belief is that Japanese Americans are passive, industrious, respectful, patient, and intelligent. They have been transformed from the "yellow peril" into the "model minority". Education featuring respect for and awareness of various ethnicities will eliminate much prejudice. (JN)
Phase-matching properties of yellow color HgGa₂S₄ for SHG and SFG in the 0.944-10.5910 μm range.

PubMed

Kato, Kiyoshi; Petrov, Valentin; Umemura, Nobuhiro

2016-04-20

We report new experimental results on the phase-matching properties of yellow color HgGa₂S₄ crystals for harmonic generation of an Nd:YAG laser-pumped KTiOPO₄ (KTP) optical parametric oscillator (OPO) and CO₂ lasers in the 0.944-10.5910 μm range. In addition, we present new Sellmeier equations that provide a good reproduction of the present experimental results as well as the published data points for second-harmonic generation of CO₂ laser radiation at 9.2197-9.6392 μm and Nd:YAG laser-pumped OPOs at 1.205-1.725 μm and 2.77-9.10 μm that were achieved with the orange and yellow color crystals.
An experimental test of the contributions and condition dependence of microstructure and carotenoids in yellow plumage coloration

PubMed Central

Shawkey, Matthew D; Hill, Geoffrey E; McGraw, Kevin J; Hood, Wendy R; Huggins, Kristal

2006-01-01

A combination of structural and pigmentary components is responsible for many of the colour displays of animals. Despite the ubiquity of this type of coloration, neither the relative contribution of structures and pigments to variation in such colour displays nor the relative effects of extrinsic factors on the structural and pigment-based components of such colour has been determined. Understanding the sources of colour variation is important because structures and pigments may convey different information to conspecifics. In an experiment on captive American goldfinches Carduelis tristis, we manipulated two parameters, carotenoid availability and food availability, known to affect the expression of carotenoid pigments in a full-factorial design. Yellow feathers from these birds were then analysed in two ways. First, we used full-spectrum spectrometry and high-performance liquid chromatography to examine the extent to which variation in white structural colour and total carotenoid content was associated with variation in colour properties of feathers. The carotenoid content of yellow feathers predicted two colour parameters (principal component 1—representing high values of ultraviolet and yellow chroma and low values of violet–blue chroma—and hue). Two different colour parameters (violet–blue and yellow chroma) from white de-pigmented feathers, as well as carotenoid content, predicted reflectance measurements from yellow feathers. Second, we determined the relative effects of our experimental manipulations on white structural colour and yellow colour. Carotenoid availability directly affected yellow colour, while food availability affected it only in combination with carotenoid availability. None of our manipulations had significant effects on the expression of white structural colour. Our results suggest that the contribution of microstructures to variation in the expression of yellow coloration is less than the contribution of carotenoid content, and

An experimental test of the contributions and condition dependence of microstructure and carotenoids in yellow plumage coloration.

PubMed

Shawkey, Matthew D; Hill, Geoffrey E; McGraw, Kevin J; Hood, Wendy R; Huggins, Kristal

2006-12-07

A combination of structural and pigmentary components is responsible for many of the colour displays of animals. Despite the ubiquity of this type of coloration, neither the relative contribution of structures and pigments to variation in such colour displays nor the relative effects of extrinsic factors on the structural and pigment-based components of such colour has been determined. Understanding the sources of colour variation is important because structures and pigments may convey different information to conspecifics. In an experiment on captive American goldfinches Carduelis tristis, we manipulated two parameters, carotenoid availability and food availability, known to affect the expression of carotenoid pigments in a full-factorial design. Yellow feathers from these birds were then analysed in two ways. First, we used full-spectrum spectrometry and high-performance liquid chromatography to examine the extent to which variation in white structural colour and total carotenoid content was associated with variation in colour properties of feathers. The carotenoid content of yellow feathers predicted two colour parameters (principal component 1--representing high values of ultraviolet and yellow chroma and low values of violet-blue chroma-and hue). Two different colour parameters (violet-blue and yellow chroma) from white de-pigmented feathers, as well as carotenoid content, predicted reflectance measurements from yellow feathers. Second, we determined the relative effects of our experimental manipulations on white structural colour and yellow colour. Carotenoid availability directly affected yellow colour, while food availability affected it only in combination with carotenoid availability. None of our manipulations had significant effects on the expression of white structural colour. Our results suggest that the contribution of microstructures to variation in the expression of yellow coloration is less than the contribution of carotenoid content, and that
Study by AES, EELS Spectroscopy of electron Irradiation on InP and InPO4/InP in comparison with Monte Carlo simulation

NASA Astrophysics Data System (ADS)

Lounis, Z.; Bouslama, M.; Hamaida, K.; Jardin, C.; Abdellaoui, A.; Ouerdane, A.; Ghaffour, M.; Berrouachedi, N.

2012-02-01

We give the great interest to characterise the InP and InPO4/InP submitted to electron beam irradiation owing to the Auger Electron Spectroscopy (AES) associated to both methods Electron Energy Loss Spectroscopy (EELS). The incident electron produces breaking of (In-P) chemical bonds. The electron beam even acts to stimulate oxidation of InP surface involving on the top layers. Other, the oxide InPO4 developed on InP does appear very sensitive to the irradiation due to electron beam shown by the monitoring of EELS spectra recorded versus the irradiated times of the surface. There appears a new oxide thought to be In2O3. We give the simulation methods Casino (Carlo simulation of electron trajectory in solids) for determination with accuracy the loss energy of backscattered electrons and compared with reports results have been obtained with EELS Spectroscopy. These techniques of spectroscopy alone do not be able to verify the affected depth during interaction process. So, using this simulation method, we determine the interaction of electrons in the matter.
Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae

PubMed Central

Mazurais, David; Servili, Arianna; Zambonino-Infante, Jose-Luis; Miest, Joanna J.; Sørensen, Sune R.; Tomkiewicz, Jonna; Butts, Ian A. E.

2017-01-01

Temperature is important for optimization of rearing conditions in aquaculture, especially during the critical early life history stages of fish. Here, we experimentally investigated the impact of temperature (16, 18, 20, 22 and 24°C) on thermally induced phenotypic variability, from larval hatch to first-feeding, and the linked expression of targeted genes [heat shock proteins (hsp), growth hormone (gh) and insulin-like growth factors (igf)] associated to larval performance of European eel, Anguilla anguilla. Temperature effects on larval morphology and gene expression were investigated throughout early larval development (in real time from 0 to 18 days post hatch) and at specific developmental stages (hatch, jaw/teeth formation, and first-feeding). Results showed that hatch success, yolk utilization efficiency, survival, deformities, yolk utilization, and growth rates were all significantly affected by temperature. In real time, increasing temperature from 16 to 22°C accelerated larval development, while larval gene expression patterns (hsp70, hsp90, gh and igf-1) were delayed at cold temperatures (16°C) or accelerated at warm temperatures (20–22°C). All targeted genes (hsp70, hsp90, gh, igf-1, igf-2a, igf-2b) were differentially expressed during larval development. Moreover, expression of gh was highest at 16°C during the jaw/teeth formation, and the first-feeding developmental stages, while expression of hsp90 was highest at 22°C, suggesting thermal stress. Furthermore, 24°C was shown to be deleterious (resulting in 100% mortality), while 16°C and 22°C (~50 and 90% deformities respectively) represent the lower and upper thermal tolerance limits. In conclusion, the high survival, lowest incidence of deformities at hatch, high yolk utilization efficiency, high gh and low hsp expression, suggest 18°C as the optimal temperature for offspring of European eel. Furthermore, our results suggest that the still enigmatic early life history stages of European
Cytotoxicity of yellow sand in lung epithelial cells.

PubMed

Kim, Y H; Kim, K S; Kwak, N J; Lee, K H; Kweon, S A; Lim, Y

2003-02-01

The present study was carried out to observe the cytotoxicity of yellow sand in comparison with silica and titanium dioxide in a rat alveolar type II cell line (RLE-6TN). Yellow sand (China Loess) was obtained from the loess layer in the Gunsu Province of China. The mean particle diameter of yellow sand was about 0.003 +/- 0.001 mm. Major elements of yellow sand were Si(27.7 +/- 0.6%), Al(6.01 +/- 0.17%), and Ca(5.83 +/- 0.23%) in that order. Silica and yellow sand significantly decreased cell viability and increased [Ca2+]i. All three particles increased the generation of H2O2. TiO2 did not change Fenton activity, while silica induced a slight increase of Fenton activity. In contrast, yellow sand induced a significant increase of Fenton activity. Silica, yellow sand and TiO2 induced significant nitrite formations in RLE-6TN cells. Silica showed the highest increase in nitrite formation, while yellow sand induced the least formation of nitrite. Silica and yellow sand increased the release of TNF-a. Based on these results, we suggest that yellow sand can induce cytotoxicity in RLE-6TN cells and reactive oxygen species, Fenton activity and reactive nitrogen species might be involved in this toxicity.
Yellow phosphorus-induced Brugada phenocopy.

PubMed

Dharanipradab, Mayakrishnan; Viswanathan, Stalin; Kumar, Gokula Raman; Krishnamurthy, Vijayalatchumy; Stanley, Daphene Divya

Metallic phosphides (of aluminum and phosphide) and yellow phosphorus are commonly used rodenticide compounds in developing countries. Toxicity of yellow phosphorus mostly pertains to the liver, kidney, heart, pancreas and the brain. Cardiotoxicity with associated Brugada ECG pattern has been reported only in poisoning with metallic phosphides. Brugada phenocopy and hepatic dysfunction were observed in a 29-year-old male following yellow phosphorus consumption. He had both type 1 (day1) and type 2 (day2) Brugada patterns in the electrocardiogram, which resolved spontaneously by the third day without hemodynamic compromise. Toxins such as aluminum and zinc phosphide have been reported to induce Brugada ECG patterns due to the generation of phosphine. We report the first case of yellow phosphorus-related Brugada phenocopy, without hemodynamic compromise or malignant arrhythmia. Copyright © 2017 Elsevier Inc. All rights reserved.
Characterization and differential expression patterns of conserved microRNAs and mRNAs in three genders of the rice field eel (Monopterus albus).

PubMed

Gao, Yu; Guo, Wei; Hu, Qing; Zou, Ming; Tang, Rong; Chi, Wei; Li, Dapeng

2014-01-01

MicroRNAs (miRNAs) are endogenous small RNAs that can regulate target mRNAs by binding to their sequences in the 3' untranslated region. The expression of miRNAs and their biogenetic pathway are involved in sexual differentiation and in the regulation of the development of germ cells and gonadal somatic cells. The rice field eel (Monopterus albus) undergoes a natural sexual transformation from female to male via an intersex stage during its life cycle. To investigate the molecular mechanisms of this sexual transformation, miRNAs present in the different sexual stages of the rice field eel were identified by high-throughput sequencing technology. A significantly differential expression among the 3 genders (p < 0.001) was observed for 48 unique miRNAs and 3 miRNAs*. Only 9 unique miRNAs showed a more than 8-fold change in their expression among the 3 genders, including mal-miR-430a and mal-miR-430c which were higher in females than in males. However, mal-miR-430b was only detected in males. Several potential miRNA target genes (cyp19a, cyp19b, nr5a1b, foxl2 amh, and vasa) were also investigated. Real-time RT-PCR demonstrated highly specific expression patterns of these genes in the 3 genders of the rice field eel. Many of these genes are targets of mal-miR-430b according to the TargetScan and miRTarBase. These results suggest that the miR-430 family may be involved in the sexual transformation of the rice field eel. © 2014 S. Karger AG, Basel.
Orange County, Yellow History: An Intimate Encounter with Vietnamese American Lives

ERIC Educational Resources Information Center

Tranguyen, Trangdai

2004-01-01

Anchored on the Vietnamese American Project (VAP), this essay presents the documentation of the Vietnamese American Experience through the voices of ethnic Vietnamese in Orange County, California, home to the largest concentration of this ethnic group outside Vietnam. Groundbreaking in its approach and method, VAP is an open forum that enables…
Perinatal Yellow Fever: A Case Report.

PubMed

Diniz, Lilian Martins Oliveira; Romanelli, Roberta Maia Castro; de Carvalho, Andréa Lucchesi; Teixeira, Daniela Caldas; de Carvalho, Luis Fernando Andrade; Cury, Verônica Ferreira; Filho, Marcelo Pereira Lima; Perígolo, Graciele; Heringer, Tiago Pires

2018-04-09

An outbreak of yellow fever in Brazil made it possible to assess different presentations of disease such as perinatal transmission. A pregnant woman was admitted to hospital with yellow fever symptoms. She was submitted to cesarean section and died due to fulminant hepatitis. On the 6th day the newborn developed liver failure and died 13 days later. Yellow fever PCR was positive for both.
Estimation of daily age and timing of hatching of exotic Asian swamp eels Monopterus albus (Zuiew, 1793) in a backwater marsh of the Chattahoochee River, Georgia, USA

USGS Publications Warehouse

Long, James M.; Lafleur, C.

2011-01-01

Otoliths were used to estimate daily age, growth, and hatching date of the exotic Asian swamp eel (Monopterus albus) captured from a backwater marsh of the Chattahoochee River, Georgia, USA. The eels were sampled using leaf litter traps (N = 140) from 17 July to 28 August 2008. The captured (N = 15) Asian swamp eels ranged in total length from 4.9 cm to 12.2 cm, and were estimated to be from 21 to 51 days old (N = 13), and hatched from 13 June to 7 August 2008. Assuming linear growth, these individuals grew an average rate of 0.2 cm per day. To the authors' knowledge, this was the first time otoliths were used to estimate daily age, growth, and hatching date for M. albus, which can be useful for understanding the ecology of this species in the wild.
Application of STEM/EELS to Plasmon-Related Effects in Optical Spectroscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Camden, Jon

In this project we employed EELS/STEM to understand the near-field enhancements that drive current applications of plasmonic nanostructures. In particular, we explore the connection between optical and electron excitation of plasmon modes in metallic nanostructures: (1) Probing the structural parameters and dielectric properties of multimetallic nanoparticles; (2) Characterization of the near-electric-field enhancements obtained upon excitation of the localized surface plasmon resonance and understand the connection between electron- and photon-driven plasmons; (3) Understanding the behavior of molecules in plasmon-enhanced fields which is essential to emerging applications such as plasmon-assisted catalysis and solar energy harvesting.
An evaluation of yellow-flowering magnolias and magnolia rootstocks

USDA-ARS?s Scientific Manuscript database

Yellow-flowering magnolias were evaluated for flower color, bloom duration and growth rate in USDA Hardiness Zone 6b. Of the thirty selections evaluated, all were reported to have yellow blooms; however, tepal color ranged from light pink with some yellow coloration, to creamy yellow to dark yellow....
Large genetic differentiation and low variation in vector competence for dengue and yellow fever viruses of Aedes albopictus from Brazil, the United States, and the Cayman Islands.

PubMed

Lourenço de Oliveira, Ricardo; Vazeille, Marie; de Filippis, Ana Maria Bispo; Failloux, Anna-Bella

2003-07-01

We conducted a population genetic analysis of Aedes albopictus collected from 20 sites in Brazil, the United States (Florida, Georgia, and Illinois), and the Cayman Islands. Using isoenzyme analysis, we examined genetic diversity and patterns of gene flow. High genetic differentiation was found among Brazilian samples, and between them and North American samples. Regression analysis of genetic differentiation according to geographic distances indicated that Ae. albopictus samples from Florida were genetically isolated by distance. Infection rates with dengue and yellow fever viruses showed greater differences between two Brazilian samples than between the two North American samples or between a Brazilian sample and a North American sample. Introductions and establishments of new Ae. albopictus populations in the Americas are still in progress, shaping population genetic composition and potentially modifying both dengue and yellow fever transmission patterns.
Comparative population genetic structure and diversity of Yellow Perch and Walleye: Broad- and fine-scale patterns across North America

USDA-ARS?s Scientific Manuscript database

The yellow perch Perca flavescens and the walleye Sander vitreus are native North American percid fishes, which have considerable fishery and ecological importance across their wide geographic ranges. Over the past century, they were stocked into new habitats, often with relative disregard for conse...
21 CFR 137.285 - Degerminated yellow corn meal.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Degerminated yellow corn meal. 137.285 Section 137... Cereal Flours and Related Products § 137.285 Degerminated yellow corn meal. Degerminated yellow corn meal, degermed yellow corn meal, conforms to the definition and standard of identity prescribed by § 137.265 for...
21 CFR 137.285 - Degerminated yellow corn meal.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Degerminated yellow corn meal. 137.285 Section 137... Cereal Flours and Related Products § 137.285 Degerminated yellow corn meal. Degerminated yellow corn meal, degermed yellow corn meal, conforms to the definition and standard of identity prescribed by § 137.265 for...
17DD yellow fever vaccine

PubMed Central

Martins, Reinaldo M.; Maia, Maria de Lourdes S.; Farias, Roberto Henrique G.; Camacho, Luiz Antonio B.; Freire, Marcos S.; Galler, Ricardo; Yamamura, Anna Maya Yoshida; Almeida, Luiz Fernando C.; Lima, Sheila Maria B.; Nogueira, Rita Maria R.; Sá, Gloria Regina S.; Hokama, Darcy A.; de Carvalho, Ricardo; Freire, Ricardo Aguiar V.; Filho, Edson Pereira; Leal, Maria da Luz Fernandes; Homma, Akira

2013-01-01

Objective: To verify if the Bio-Manguinhos 17DD yellow fever vaccine (17DD-YFV) used in lower doses is as immunogenic and safe as the current formulation. Results: Doses from 27,476 IU to 587 IU induced similar seroconversion rates and neutralizing antibodies geometric mean titers (GMTs). Immunity of those who seroconverted to YF was maintained for 10 mo. Reactogenicity was low for all groups. Methods: Young and healthy adult males (n = 900) were recruited and randomized into 6 groups, to receive de-escalating doses of 17DD-YFV, from 27,476 IU to 31 IU. Blood samples were collected before vaccination (for neutralization tests to yellow fever, serology for dengue and clinical chemistry), 3 to 7 d after vaccination (for viremia and clinical chemistry) and 30 d after vaccination (for new yellow fever serology and clinical chemistry). Adverse events diaries were filled out by volunteers during 10 d after vaccination. Volunteers were retested for yellow fever and dengue antibodies 10 mo later. Seropositivity for dengue was found in 87.6% of volunteers before vaccination, but this had no significant influence on conclusions. Conclusion: In young healthy adults Bio-Manguinhos/Fiocruz yellow fever vaccine can be used in much lower doses than usual. International Register ISRCTN 38082350. PMID:23364472
Newly documented host fishes for the eastern elliptio mussel (Elliptio complanata)

USGS Publications Warehouse

Galbraith, Heather S.

2013-01-01

The eastern elliptio (Elliptio complanata) is a common, abundant and ecologically important freshwater mussel that occurs throughout the Atlantic Slope drainage in the United States and Canada. Previous research has shown E. complanata glochidia to be host fish generalists, parasitizing yellow perch (Perca flavescens), banded killifish (Fundulus diaphanus), banded sculpin (Cottus carolinae), and seven centrarchid species. Past laboratory studies have been conducted in the Midwest and glochidia sources typically included lakes the Great Lakes basin or were unreported. The objective of this study was to identify host fishes for E. complanata from streams in the Mid-Atlantic region. We used artificial laboratory infections to test host suitability of 38 fish and two amphibian species with E. complanata glochidia from the Chesapeake Bay drainage. Glochidia successfully metamorphosed into juvenile mussels on five fish species: American eel (Anguilla rostrata), brook trout (Salvelinus fontinalis), lake trout (S. namaycush), mottled sculpin (C. bairdii), and slimy sculpin (C. cognatus). American eel was the most effective host, yielding the highest overall metamorphosis success (percentage of attached glochidia that transformed into juvenile mussels;{greater than or equal to}0.90) and producing 13.2 juveniles per fish overall. No juvenile E. complanata metamorphosed on other fish species tested, including many previously identified host fishes reported in the literature. Reasons for discrepancies in published host fish could include geographic variation in host use across the species' range, differences in host use between lentic and lotic populations, or poorly resolved taxonomy within the genus Elliptio.
Preference of Formosan subterranean termites for blue-stained southern yellow pine sapwood.

PubMed

Little, N S; Blount, N A; Londo, A J; Kitchens, S C; Schultz, T P; McConnell, T E; Riggins, J J

2012-10-01

Little research has been conducted to investigate interactions between the invasive Formosan subterranean termite, Coptotermes formosanus Shiraki, and pine bark beetles native to the southeastern United States. Facilitative interactions between these organisms could alter stand dynamics and impact wood utilization strategies. American Wood Protection Association Standard E1-09 choice tests were carried out to determine the feeding preference of Formosan subterranean termites for blue-stained versus unstained southern yellow pine sapwood. Three separate colonies of Formosan subterranean termites consumed on average twice as much air-dried blue-stained southern yellow pine sapwood over unstained air-dried controls. Additionally, Formosan subterranean termites consumed over five-times more kiln-dried blue-stained sapwood than unstained kiln-dried control wafers. The implications of these results are particularly relevant to pine forest ecology, nutrient cycling, and the utilization of blue-stained southern pine building products in the southeastern United States, where Formosan subterranean termites have become established.
Yellow-Poplar Site Index Curves

Treesearch

Donald E. Beck

1962-01-01

Yellow-poplar (Liriodendron tulipifera L.) occurs naturally throughout the eastern and central United States from southern New England west to Michigan and south to Florida and Louisiana. Because of its wide occurrence, yellow-poplar grows under a variety of climatic, edaphic, and biotic conditions. Combinations of these different environmental...
Yellow light generation by frequency doubling of a diode-pumped Nd:YAG laser

NASA Astrophysics Data System (ADS)

Jia, Fu-qiang; Zheng, Quan; Xue, Qing-hua; Bu, Yi-kun; Qian, Long-sheng

2006-03-01

We demonstrate the generation of TEM00 mode yellow light in critically type II phase-matched KTiOPO4 (KTP) with intracavity frequency doubling of a diode-pumped Nd:YAG laser at room temperature. After a 150 μm thick etalon have been inserted into the cavity, the stability and beam quality of the second harmonic generation (SHG) is enhanced. A continuous wave (CW) TEM00 mode output power of 1.67 W at 556 nm is obtained at a pump level of 16 W. The total optical to optical conversion efficiency is about 10.44%. To the best of our knowledge, this is the first Watt-level yellow light generation by frequency doubling of Nd:YAG laser.

38 CFR 21.9700 - Yellow Ribbon Program.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2011-07-01 2011-07-01 false Yellow Ribbon Program. 21... Ribbon Program. (a) Establishment. The “Yellow Ribbon G.I. Education Enhancement Program”, known as the “Yellow Ribbon Program,” permits an institution of higher learning (IHL), at the IHL's option, to enter...
38 CFR 21.9700 - Yellow Ribbon Program.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2013-07-01 2013-07-01 false Yellow Ribbon Program. 21... Ribbon Program. (a) Establishment. The “Yellow Ribbon G.I. Education Enhancement Program”, known as the “Yellow Ribbon Program,” permits an institution of higher learning (IHL), at the IHL's option, to enter...
38 CFR 21.9700 - Yellow Ribbon Program.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Yellow Ribbon Program. 21... Ribbon Program. (a) Establishment. The “Yellow Ribbon G.I. Education Enhancement Program”, known as the “Yellow Ribbon Program,” permits an institution of higher learning (IHL), at the IHL's option, to enter...
38 CFR 21.9700 - Yellow Ribbon Program.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2014-07-01 2014-07-01 false Yellow Ribbon Program. 21... Ribbon Program. (a) Establishment. The “Yellow Ribbon G.I. Education Enhancement Program”, known as the “Yellow Ribbon Program,” permits an institution of higher learning (IHL), at the IHL's option, to enter...
38 CFR 21.9700 - Yellow Ribbon Program.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Yellow Ribbon Program. 21... Ribbon Program. (a) Establishment. The “Yellow Ribbon G.I. Education Enhancement Program”, known as the “Yellow Ribbon Program,” permits an institution of higher learning (IHL), at the IHL's option, to enter...
Yellow fever virus in Haemagogus leucocelaenus and Aedes serratus mosquitoes, southern Brazil, 2008.

PubMed

Cardoso, Jader da C; de Almeida, Marco A B; dos Santos, Edmilson; da Fonseca, Daltro F; Sallum, Maria A M; Noll, Carlos A; Monteiro, Hamilton A de O; Cruz, Ana C R; Carvalho, Valeria L; Pinto, Eliana V; Castro, Francisco C; Nunes Neto, Joaquim P; Segura, Maria N O; Vasconcelos, Pedro F C

2010-12-01

Yellow fever virus (YFV) was isolated from Haemagogus leucocelaenus mosquitoes during an epizootic in 2001 in the Rio Grande do Sul State in southern Brazil. In October 2008, a yellow fever outbreak was reported there, with nonhuman primate deaths and human cases. This latter outbreak led to intensification of surveillance measures for early detection of YFV and support for vaccination programs. We report entomologic surveillance in 2 municipalities that recorded nonhuman primate deaths. Mosquitoes were collected at ground level, identified, and processed for virus isolation and molecular analyses. Eight YFV strains were isolated (7 from pools of Hg. leucocelaenus mosquitoes and another from Aedes serratus mosquitoes); 6 were sequenced, and they grouped in the YFV South American genotype I. The results confirmed the role of Hg. leucocelaenus mosquitoes as the main YFV vector in southern Brazil and suggest that Ae. serratus mosquitoes may have a potential role as a secondary vector.
Fatal Yellow Fever in Travelers to Brazil, 2018.

PubMed

Hamer, Davidson H; Angelo, Kristina; Caumes, Eric; van Genderen, Perry J J; Florescu, Simin A; Popescu, Corneliu P; Perret, Cecilia; McBride, Angela; Checkley, Anna; Ryan, Jenny; Cetron, Martin; Schlagenhauf, Patricia

2018-03-23

Yellow fever virus is a mosquito-borne flavivirus that causes yellow fever, an acute infectious disease that occurs in South America and sub-Saharan Africa. Most patients with yellow fever are asymptomatic, but among the 15% who develop severe illness, the case fatality rate is 20%-60%. Effective live-attenuated virus vaccines are available that protect against yellow fever (1). An outbreak of yellow fever began in Brazil in December 2016; since July 2017, cases in both humans and nonhuman primates have been reported from the states of São Paulo, Minas Gerais, and Rio de Janeiro, including cases occurring near large urban centers in these states (2). On January 16, 2018, the World Health Organization updated yellow fever vaccination recommendations for Brazil to include all persons traveling to or living in Espírito Santo, São Paulo, and Rio de Janeiro states, and certain cities in Bahia state, in addition to areas where vaccination had been recommended before the recent outbreak (3). Since January 2018, 10 travel-related cases of yellow fever, including four deaths, have been reported in international travelers returning from Brazil. None of the 10 travelers had received yellow fever vaccination.
Yellow fever disease: density equalizing mapping and gender analysis of international research output.

PubMed

Bundschuh, Matthias; Groneberg, David A; Klingelhoefer, Doris; Gerber, Alexander

2013-11-18

A number of scientific papers on yellow fever have been published but no broad scientometric analysis on the published research of yellow fever has been reported.The aim of the article based study was to provide an in-depth evaluation of the yellow fever field using large-scale data analysis and employment of bibliometric indicators of production and quantity. Data were retrieved from the Web of Science database (WoS) and analyzed as part of the NewQis platform. Then data were extracted from each file, transferred to databases and visualized as diagrams. Partially by means of density-equalizing mapping makes the findings clear and emphasizes the output of the analysis. In the study period from 1900 to 2012 a total of 5,053 yellow fever-associated items were published by 79 countries. The United States (USA) having the highest publication rate at 42% (n = 751) followed by far from Brazil (n = 203), France (n = 149) and the United Kingdom (n = 113). The most productive journals are the "Public Health Reports", the "American Journal of Tropical Medicine and Hygiene" and the "Journal of Virology". The gender analysis showed an overall steady increase of female authorship from 1950 to 2011. Brazil is the only country of the five most productive countries with a higher proportion of female scientists. The present data shows an increase in research productivity over the entire study period, in particular an increase of female scientists. Brazil shows a majority of female authors, a fact that is confirmed by other studies.
Yield of Unthinned Yellow-Poplar

Treesearch

Donald E. Beck; Lino Della-Bianca

1970-01-01

Cubic-foot and board-foot yields of unthinned yellow-poplar (Liriodendron Tulipiferi L.) stands are described in relation to stand age, site index, and number of trees per acre. The yield tables are based on analysis of diameter distributions and height-diameter relationships obtained from 141 natural, unthinned yellow-poplar stands in the...
Diel and seasonal movements by adult Sacramento pikeminnow (Ptychocheilus grandis) in the Eel River, northwestern California

Treesearch

Bret C. Harvey; Rodney J. Nakamoto

1999-01-01

Abstract - In late summer and fall, radio-tagged adult Sacramento pike-minnow (Ptychocheilus grandis) at three sites in the Eel River of northwestern California moved more at night than during the day. Fish moved up to 535 m at night and returned to their original positions the following morning. Adult Sacramento pikeminnow at all sites occupied only pools during the...
Estimation of daily age and timing of hatching of exotic Asian swamp eels Monopterus albus (Zuiew, 1793) in a backwater marsh of the Chattahoochee River, Georgia, USA

USGS Publications Warehouse

Long, J.M.; Lafleur, C.

2011-01-01

Otoliths were used to estimate daily age, growth, and hatching date of the exotic Asian swamp eel (Monopterus albus) captured from a backwater marsh of the Chattahoochee River, Georgia, USA. The eels were sampled using leaf litter traps (N=140) from 17 July to 28 August 2008. The captured (N=15) Asian swamp eels ranged in total length from 4.9cm to 12.2cm, and were estimated to be from 21 to 51days old (N=13), and hatched from 13 June to 7 August 2008. Assuming linear growth, these individuals grew an average rate of 0.2cm per day. To the authors' knowledge, this was the first time otoliths were used to estimate daily age, growth, and hatching date for M. albus, which can be useful for understanding the ecology of this species in the wild. Published 2011. This article is a US Government work and is in the public domain in the USA.
Biofortified yellow cassava and vitamin A status of Kenyan children: a randomized controlled trial.

PubMed

Talsma, Elise F; Brouwer, Inge D; Verhoef, Hans; Mbera, Gloria N K; Mwangi, Alice M; Demir, Ayşe Y; Maziya-Dixon, Busie; Boy, Erick; Zimmermann, Michael B; Melse-Boonstra, Alida

2016-01-01

Whereas conventional white cassava roots are devoid of provitamin A, biofortified yellow varieties are naturally rich in β-carotene, the primary provitamin A carotenoid. We assessed the effect of consuming yellow cassava on serum retinol concentration in Kenyan schoolchildren with marginal vitamin A status. We randomly allocated 342 children aged 5-13 y to receive daily, 6 d/wk, for 18.5 wk 1) white cassava and placebo supplement (control group), 2) provitamin A-rich cassava (mean content: 1460 μg β-carotene/d) and placebo supplement (yellow cassava group), and 3) white cassava and β-carotene supplement (1053 μg/d; β-carotene supplement group). The primary outcome was serum retinol concentration; prespecified secondary outcomes were hemoglobin concentration and serum concentrations of β-carotene, retinol-binding protein, and prealbumin. Groups were compared by using ANCOVA, adjusting for inflammation, baseline serum concentrations of retinol and β-carotene, and stratified design. The baseline prevalence of serum retinol concentration <0.7 μmol/L and inflammation was 27% and 24%, respectively. For children in the control, yellow cassava, and β-carotene supplement groups, the mean daily intake of cassava was 378, 371, and 378 g, respectively, and the total daily supply of provitamin A and vitamin A from diet and supplements was equivalent to 22, 220, and 175 μg retinol, respectively. Both yellow cassava and β-carotene supplementation increased serum retinol concentration by 0.04 μmol/L (95% CI: 0.00, 0.07 μmol/L); correspondingly, serum β-carotene concentration increased by 524% (448%, 608%) and 166% (134%, 202%). We found no effect on hemoglobin concentration or serum concentrations of retinol-binding protein and prealbumin. In our study population, consumption of yellow cassava led to modest gains in serum retinol concentration and a large increase in β-carotene concentration. It can be an efficacious, new approach to improve vitamin A status. This
An inactivated cell-culture vaccine against yellow fever.

PubMed

Monath, Thomas P; Fowler, Elizabeth; Johnson, Casey T; Balser, John; Morin, Merribeth J; Sisti, Maggie; Trent, Dennis W

2011-04-07

Yellow fever is a lethal viral hemorrhagic fever occurring in Africa and South America. A highly effective live vaccine (17D) is widely used for travelers to and residents of areas in which yellow fever is endemic, but the vaccine can cause serious adverse events, including viscerotropic disease, which is associated with a high rate of death. A safer, nonreplicating vaccine is needed. In a double-blind, placebo-controlled, dose-escalation, phase 1 study of 60 healthy subjects between 18 and 49 years of age, we investigated the safety and immunogenicity of XRX-001 purified whole-virus, β-propiolactone-inactivated yellow fever vaccine produced in Vero cell cultures and adsorbed to aluminum hydroxide (alum) adjuvant. On two visits 21 days apart, subjects received intramuscular injections of vaccine that contained 0.48 μg or 4.8 μg of antigen. Levels of neutralizing antibodies were measured at baseline and on days 21, 31, and 42. The vaccine induced the development of neutralizing antibodies in 100% of subjects receiving 4.8 μg of antigen in each injection and in 88% of subjects receiving 0.48 μg of antigen in each injection. Antibody levels increased by day 10 after the second injection, at which time levels were significantly higher with the 4.8-μg formulation than with the 0.48-μg formulation (geometric mean titer, 146 vs. 39; P<0.001). Three adverse events occurred at a higher incidence in the two vaccine groups than in the placebo group: mild pain, tenderness, and (much less frequently) itching at the injection site. One case of urticaria was observed on day 3 after the second dose of 4.8 μg of vaccine. A two-dose regimen of the XRX-001 vaccine, containing inactivated yellow fever antigen with an alum adjuvant, induced neutralizing antibodies in a high percentage of subjects. XRX-001 has the potential to be a safer alternative to live attenuated 17D vaccine. (Funded by Xcellerex; ClinicalTrials.gov number, NCT00995865.).
Plant Guide: Yellow beeplant (Cleome lutea Hook)

Treesearch

Derek Tilley; Jim Cane; Loren St. John; Dan Ogle; Nancy Shaw

2012-01-01

Yellow beeplant is a valuable native forage species for bees wasps and butterflies. Over 140 species of native bees have been observed foraging for nectar or pollen on yellow beeplant in southern Utah (Cane, 2008). Yellow beeplant is an annual forb which could provide food to insects in the first growing season of a range seeding (Ogle and others, 2011a). This...
How do eggs get fat? Insights into ovarian fatty acid accumulation in the shortfinned eel, Anguilla australis.

PubMed

Damsteegt, Erin L; Mizuta, Hiroko; Hiramatsu, Naoshi; Lokman, P Mark

2015-09-15

Previous research using eels has shown that 11-ketotestosterone can induce ovarian triacylglyceride accumulation both in vivo and in vitro. Further, accumulation is dramatically enhanced in the presence of very-low density lipoprotein. This study examined the involvement of the low density lipoprotein receptor and vitellogenin receptor in oocyte lipid accumulation. Specific antisera were used in an attempt to block the vitellogenin receptor and/or the low density lipoprotein receptor. Accordingly, incubation with the low density lipoprotein receptor antiserum clearly reduced the oocyte diameter and the amount of oil present within the oocyte. In contrast, blocking the vitellogenin receptor had little effect on either oocyte surface area or the abundance of oil droplets in the cytosol. In keeping with birds, we conclude that the low density lipoprotein receptor is a major player involved in mediating ovarian fatty acid accumulation in the eel. However, lipoprotein lipase-mediated fatty acid accumulation also remains conceivable, for example through interactions between this enzyme and the low density lipoprotein receptor. Copyright © 2015 Elsevier Inc. All rights reserved.
TEM-EELS Investigation of Boron and Phosphorus Passivated 4H-SiC/SiO2 Interface Structures

NASA Astrophysics Data System (ADS)

Klingshirn, Christopher; Taillon, Joshua; Liu, Gang; Dhar, Sarit; Feldman, Leonard; Zheleva, Tsvetanka; Lelis, Aivars; Salamanca-Riba, Lourdes

A high density of electronic defects at the SiC/SiO2 interface adversely affects SiC-based metal oxide semiconductor devices. Various treatments are known to improve device performance. Annealing in a nitric oxide (NO) environment, for example, passivates electronic defects at the interface and raises the carrier mobility in the active region to 35-40 cm2/Vs, but the effect saturates after about 60 minutes of annealing. Passivation with phosphorus or boron improves upon NO by a factor of 2, increasing the mobility to over 90 cm2/Vs.2 We investigate the chemical and structural effects of these treatments on the SiC/SiO2 transition layer using high-resolution transmission electron microscopy (HRTEM) and high angle annular dark field (HAADF). Electron energy loss spectroscopy Spectrum Imaging (EELS SI) collected across the transition region allow identification of the width, composition and types of bonding at the transition layer. Advanced machine learning techniques applied to the EELS data reveal intermediate bonding states within this region. Supported by ARL under Grant No. W911NF1420110.
Swimming physiology of European silver eels (Anguilla anguilla L.): energetic costs and effects on sexual maturation and reproduction.

PubMed

Palstra, Arjan P; van den Thillart, Guido E E J M

2010-09-01

The European eel migrates 5,000-6,000 km to the Sargasso Sea to reproduce. Because they venture into the ocean in a pre-pubertal state and reproduce after swimming for months, a strong interaction between swimming and sexual maturation is expected. Many swimming trials have been performed in 22 swim tunnels to elucidate their performance and the impact on maturation. European eels are able to swim long distances at a cost of 10-12 mg fat/km which is 4-6 times more efficient than salmonids. The total energy costs of reproduction correspond to 67% of the fat stores. During long distance swimming, the body composition stays the same showing that energy consumption calculations cannot be based on fat alone but need to be compensated for protein oxidation. The optimal swimming speed is 0.61-0.67 m s(-1), which is approximately 60% higher than the generally assumed cruise speed of 0.4 m s(-1) and implies that female eels may reach the Sargasso Sea within 3.5 months instead of the assumed 6 months. Swimming trials showed lipid deposition and oocyte growth, which are the first steps of sexual maturation. To investigate effects of oceanic migration on maturation, we simulated group-wise migration in a large swim-gutter with seawater. These trials showed suppressed gonadotropin expression and vitellogenesis in females, while in contrast continued sexual maturation was observed in silver males. The induction of lipid deposition in the oocytes and the inhibition of vitellogenesis by swimming in females suggest a natural sequence of events quite different from artificial maturation protocols.
Swimming physiology of European silver eels (Anguilla anguilla L.): energetic costs and effects on sexual maturation and reproduction

PubMed Central

van den Thillart, Guido E. E. J. M.

2010-01-01

The European eel migrates 5,000–6,000 km to the Sargasso Sea to reproduce. Because they venture into the ocean in a pre-pubertal state and reproduce after swimming for months, a strong interaction between swimming and sexual maturation is expected. Many swimming trials have been performed in 22 swim tunnels to elucidate their performance and the impact on maturation. European eels are able to swim long distances at a cost of 10–12 mg fat/km which is 4–6 times more efficient than salmonids. The total energy costs of reproduction correspond to 67% of the fat stores. During long distance swimming, the body composition stays the same showing that energy consumption calculations cannot be based on fat alone but need to be compensated for protein oxidation. The optimal swimming speed is 0.61–0.67 m s−1, which is ~60% higher than the generally assumed cruise speed of 0.4 m s−1 and implies that female eels may reach the Sargasso Sea within 3.5 months instead of the assumed 6 months. Swimming trials showed lipid deposition and oocyte growth, which are the first steps of sexual maturation. To investigate effects of oceanic migration on maturation, we simulated group-wise migration in a large swim-gutter with seawater. These trials showed suppressed gonadotropin expression and vitellogenesis in females, while in contrast continued sexual maturation was observed in silver males. The induction of lipid deposition in the oocytes and the inhibition of vitellogenesis by swimming in females suggest a natural sequence of events quite different from artificial maturation protocols. PMID:20390348
A transmission electron microscopy study of Fe-Co alloy nanoparticles in silica aerogel matrix using HREM, EDX, and EELS.

PubMed

Falqui, Andrea; Corrias, Anna; Gass, Mhairi; Mountjoy, Gavin

2009-04-01

Magnetic nanocomposite materials consisting of 5.5 wt% Fe-Co alloy nanoparticles in a silica aerogel matrix, with compositions Fe(x)Co(1-x) of x = 0.50 and 0.67, have been synthesized by the sol-gel method. The high-resolution transmission electron microscopy images show nanoparticles consisting of single crystal grains of body-centered cubic Fe-Co alloy, with typical crystal grain diameters of approximately 4 and 7 nm for Fe(0.5)Co(0.5) and Fe(0.67)Co(0.33) samples, respectively. The energy dispersive X-ray (EDX) spectra summed over areas of the samples gave compositions Fe(x)C(o1-x) with x = 0.48 +/- 0.06 and 0.68 +/- 0.05. The EDX spectra obtained with the 1.5 nm probe positioned at the centers of approximately 20 nanoparticles gave slightly lower concentrations of Fe, with means of x = 0.43 +/- 0.01 and x = 0.64 +/- 0.02, respectively. The Fe(0.5)Co(0.50) sample was studied using electron energy loss spectroscopy (EELS), and EELS spectra summed over whole nanoparticles gave x = 0.47 +/- 0.06. The EELS spectra from analysis profiles of nanoparticles show a distribution of Fe and Co that is homogeneous, i.e., x = 0.5, within a precision of at best +/-0.05 in x and +/-0.4 nm in position. The present microscopy results have not shown the presence of a thin layer of iron oxide, but this might be at the limit of detectability of the methods.
Status assessment and conservation plan for the yellow-billed loon (Gavia adamsii)

USGS Publications Warehouse

Earnst, Susan L.

2004-01-01

Because of its restricted range, small population size, specific habitat requirements, and perceived threats to its breeding habitat, the Yellow-billed Loon (Gavia adamsii) is a species of conservation concern to the U.S. Fish and Wildlife Service and the subject of a petition for listing under the Endangered Species Act. This Status Assessment synthesizes current information on population size, trends, and potential threats to Yellow-billed Loons, and the Conservation Plan identifies research and monitoring activities that would contribute to the conservation of this species. The preparation of this report was requested and funded by the U.S. Fish and Wildlife Service, Nongame Bird Office, Region 7.The Status Assessment and Conservation Plan for the Yellow-billed Loon can be summarized as follows:? Northern Alaska breeding grounds support an average of 3,369 individuals, including <1,000 nesting pairs in most years. The Yellow-billed Loon ranks as one of the 10 rarest birds that breeds regularly within the main land U.S. and one of only 20 with a North American population <16,000 individuals (Section 6-E).? There is no evidence of a long-term trend in the Yellow-billed Loon population index since 1986 (-0.9% annual change), but interpretation of surveys is complicated by changes in observers and high annual variation, and the 95% confidence interval is large (-3.6% to +1.8% annual change). The low reproductive potential of Yellow-billed Loons suggests that recovery from a substantial decline would not occur rapidly. There are no systematic surveys of Canadian and Russian breeding populations (Section 6-F).? The expansion of the oil industry into prime Yellow-billed Loon breeding habitat is a recent occurrence and we lack the necessary information to accurately predict its effect on the population. Most of northern Alaska?s Yellow-billed Loons (91%) occur on the National Petroleum Reserve?Alaska, virtually all of which is open or proposed to be opened to

Why is my alfalfa yellow?

USDA-ARS?s Scientific Manuscript database

In 2016, many parts of the Midwest experienced far wetter than normal summer weather and by August or September, many growers were asking, “Why is my alfalfa yellow?” When all or part of an alfalfa field is yellow, it is a certain sign that something has gone wrong. In this case the problem in most ...
Financial maturity of yellow birch

Treesearch

William B. Leak

1969-01-01

The methods used to compute financial maturity of yellow birch sawtimber are similar to those used for paper birch sawtimber, except for minor differences in detail. The procedure followed for yellow-birch veneer-log trees was also similar, except that local veneer grades and local veneer-log prices were used as the basis for the financial maturity computations.
[Cloning and expression analysis of two pro-inflammatory cytokines, IL-1β and its receptor, IL-1R2, in the Asian swamp eel Monopterus albus].

PubMed

Xu, Q Q; Xu, P; Zhou, J W; Pan, T S; Tuo, R; Ai, K; Yang, D Q

2016-01-01

Interleukin-1β (IL-1β) is the prototypic pro-inflammatory cytokine, whose functions are mediated through interaction with its receptors (IL-1R1 and IL-1R2). Herein, we cloned the full-length cDNA and genomic DNA of IL-1β and IL-1R2 in the Asian swamp eel (Monopterus albus). The eel IL-1β cDNA encodes a putative polypeptide of 246 amino acids. The protein sequence includes a typical IL-1 family signature, but lacked an interleukin-converting enzyme cleavage site. The genomic DNA of eel IL-1β was 2520 bp and comprised five exons and four introns. The eel IL-1R2 cDNA encoded a putative propeptide of 423 amino acid residues, comprising a signal peptide, a transmembrane region and two Ig-like domains in the extracellular region. Similar to other vertebrates, the genomic DNA of the eel IL-1R2 has nine exons and eight introns. Real-time PCR analysis indicated that IL-1β and IL-1R2 were constitutively expressed in all tissues, especially in the liver and immune-related organs. After infection with Aeromonas hydrophila, the transcript levels of IL-1β and IL-1R2 were induced in the head kidney and spleen, reaching their highest levels at 6 h post injection. In vitro, IL-1β and IL-1R2 mRNA levels were also upregulated rapidly at 1h post infection with A. hydrophila. Furthermore, acanthocephalan Pallisentis (Neosentis) celatus could induce the expression of both genes in the head kidney and intestine. In infected intestines, the transcript levels of IL-1β and IL-1R2 were increased by 21.4-fold and 20.8-fold, respectively, relative to the control. The present study indicated that IL-1β and IL-1R2 play an important role in inflammation and host defense, especially in the antiacanthocephalan response.
Site-specific electronic structure analysis by channeling EELS and first-principles calculations.

PubMed

Tatsumi, Kazuyoshi; Muto, Shunsuke; Yamamoto, Yu; Ikeno, Hirokazu; Yoshioka, Satoru; Tanaka, Isao

2006-01-01

Site-specific electronic structures were investigated by electron energy loss spectroscopy (EELS) under electron channeling conditions. The Al-K and Mn-L(2,3) electron energy loss near-edge structure (ELNES) of, respectively, NiAl2O4 and Mn3O4 were measured. Deconvolution of the raw spectra with the instrumental resolution function restored the blunt and hidden fine features, which allowed us to interpret the experimental spectral features by comparing with theoretical spectra obtained by first-principles calculations. The present method successfully revealed the electronic structures specific to the differently coordinated cationic sites.
Yellow lights : small changes in the timing of signal lights could reduce crashes at urban intersections

DOT National Transportation Integrated Search

2001-04-28

An Institute of Transportation Engineers (ITE) test at 122 standard four-leg intersections in Long Island, NY, shows that retiming the length of the yellow/red phases of traffic signals can reduce serious and reportable accidents ($1,000-plus damage ...
Alder Establishment and Channel Dynamics in a Tributary of the South Fork Eel River, Mendocino County, California

Treesearch

William J. Trush; Edward C. Connor; Knight Alan W.

1989-01-01

Riparian communities established along Elder Creek, a tributary of the upper South Fork Eel River, are bounded by two frequencies of periodic flooding. The upper limit for the riparian zone occurs at bankfull stage. The lower riparian limit is associated with a more frequent stage height, called the active channel, having an exceedance probability of 11 percent on a...
Viscerotropic disease following yellow fever vaccination in Peru.

PubMed

Whittembury, Alvaro; Ramirez, Gladys; Hernández, Herminio; Ropero, Alba Maria; Waterman, Steve; Ticona, María; Brinton, Margo; Uchuya, Jorge; Gershman, Mark; Toledo, Washington; Staples, Erin; Campos, Clarense; Martínez, Mario; Chang, Gwong-Jen J; Cabezas, Cesar; Lanciotti, Robert; Zaki, Sherif; Montgomery, Joel M; Monath, Thomas; Hayes, Edward

2009-10-09

Five suspected cases of yellow fever vaccine-associated viscerotropic disease (YEL-AVD) clustered in space and time following a vaccination campaign in Ica, Peru in 2007. All five people received the same lot of 17DD live attenuated yellow fever vaccine before their illness; four of the five died of confirmed YEL-AVD. The surviving case was classified as probable YEL-AVD. Intensive investigation yielded no abnormalities of the implicated vaccine lot and no common risk factors. This is the first described space-time cluster of yellow fever viscerotropic disease involving more than two cases. Mass yellow fever vaccination should be avoided in areas that present extremely low risk of yellow fever.
Yellow Fever Virus in Haemagogus leucocelaenus and Aedes serratus Mosquitoes, Southern Brazil, 2008

PubMed Central

Cardoso, Jáder da C.; de Almeida, Marco A.B.; dos Santos, Edmilson; da Fonseca, Daltro F.; Sallum, Maria A.M.; Noll, Carlos A.; Monteiro, Hamilton A. de O.; Cruz, Ana C.R.; Carvalho, Valéria L.; Pinto, Eliana V.; Castro, Francisco C.; Neto, Joaquim P. Nunes; Segura, Maria N.O.

2010-01-01

Yellow fever virus (YFV) was isolated from Haemagogus leucocelaenus mosquitoes during an epizootic in 2001 in the Rio Grande do Sul State in southern Brazil. In October 2008, a yellow fever outbreak was reported there, with nonhuman primate deaths and human cases. This latter outbreak led to intensification of surveillance measures for early detection of YFV and support for vaccination programs. We report entomologic surveillance in 2 municipalities that recorded nonhuman primate deaths. Mosquitoes were collected at ground level, identified, and processed for virus isolation and molecular analyses. Eight YFV strains were isolated (7 from pools of Hg. leucocelaenus mosquitoes and another from Aedes serratus mosquitoes); 6 were sequenced, and they grouped in the YFV South American genotype I. The results confirmed the role of Hg. leucocelaenus mosquitoes as the main YFV vector in southern Brazil and suggest that Ae. serratus mosquitoes may have a potential role as a secondary vector. PMID:21122222
Occurrence of perfluorooctanesulfonate and perfluorooctanoic acid and histopathology in eels from north Italian waters.

PubMed

Giari, Luisa; Guerranti, Cristiana; Perra, Guido; Lanzoni, Mattia; Fano, Elisa Anna; Castaldelli, Giuseppe

2015-01-01

A perfluorinated alkylated substances (PFAS) biomonitoring study was conducted in European eel (Anguilla anguilla) in Italy for the first time. Perfluorooctanesulfonate (PFOS) and perfluorooctanoic acid (PFOA) concentrations were assessed in the organs of 35 wild eels from two locations, the highly impacted Po River and the Comacchio Lagoon along the north-western Adriatic coast. PFAS were extracted by ion-pairing liquid extraction procedure and measured using high performance liquid chromatography with electrospray ionization tandem mass spectrometry. There were no significant differences in mean PFAS concentrations (p>0.05) between samples from the two sites. PFOS and PFOA were detectable (>0.4ngg(-1) wet weight, w.w) in 73% and 31% of the total samples, respectively. PFOS concentrations ranged from <0.4 to 6.28ngg(-1)w.w and PFOA from <0.4 to 92.77ngg(-1)w.w. The highest PFAS levels were observed in blood and the lowest in muscle. Histology showed macrophage aggregates and hepatocytic vacuolation in some liver samples. No tissue anomalies were seen in the gonads, suggesting no reproductive impairment. The PFAS contamination levels observed were comparable to, or lower than, those reported in fish in other European countries, seeming to indicate that PFAS pollution of the study area is not remarkable. Copyright © 2014 Elsevier Ltd. All rights reserved.
Perfluoroalkyl and polyfluoroalkyl substances in the lower atmosphere and surface waters of the Chinese Bohai Sea, Yellow Sea, and Yangtze River estuary.

PubMed

Zhao, Zhen; Tang, Jianhui; Mi, Lijie; Tian, Chongguo; Zhong, Guangcai; Zhang, Gan; Wang, Shaorui; Li, Qilu; Ebinghaus, Ralf; Xie, Zhiyong; Sun, Hongwen

2017-12-01

Polyfluoroalkyl and perfluoroalkyl substances (PFASs), in the forms of neutral polyfluoroalkyl substances in the gas phase of air and ionic perfluoroalkyl substances in the dissolved phase of surface water, were investigated during a sampling campaign in the Bohai Sea, Yellow Sea, and Yangtze River estuary in May 2012. In the gas phase, the concentrations of neutral ∑PFASs were within the range of 76-551pg/m 3 . Higher concentrations were observed in the South Yellow Sea. 8:2 fluorotelomer alcohol (FTOH) was the predominant compound as it accounted for 92%-95% of neutral ∑PFASs in all air samples. Air mass backward trajectory analysis indicated that neutral ∑PFASs came mainly from the coast of the Yellow Sea, including the Shandong, Jiangsu, and Zhejiang provinces of China, and the coastal region of South Korea. The fluxes of gas phase dry deposition were simulated for neutral PFASs, and neutral ∑PFASs fluxes varied from 0.37 to 2.3pg/m 2 /s. In the dissolved phase of the surface water, concentrations of ionic ∑PFASs ranged from 1.6 to 118ng/L, with the Bohai Sea exhibiting higher concentrations than both the Yellow Sea and the Yangtze River estuary. Perfluorooctanoic acid (PFOA) was the predominant compound accounting for 51%-90% of the ionic ∑PFAS concentrations. Releases from industrial and domestic activities as well as the semiclosed geographical conditions increased the level of ionic ∑PFASs in the Bohai Sea. The spatial distributions of perfluoroalkyl carboxylic acids (PFCAs) and perfluoroalkane sulfonic acids (PFSAs) were different significantly. The Laizhou Bay was the major source region of PFCAs and the Yangtze River estuary was the major source of PFSAs. Copyright © 2017 Elsevier B.V. All rights reserved.
[Effect of transparent yellow and orange colored contact lenses on color discrimination in the yellow color range].

PubMed

Schürer, M; Walter, A; Brünner, H; Langenbucher, A

2015-08-01

Colored transparent filters cause a change in color perception and have an impact on the perceptible amount of different colors and especially on the ability to discriminate between them. Yellow or orange tinted contact lenses worn to enhance contrast vision by reducing or blocking short wavelengths also have an effect on color perception. The impact of the yellow and orange tinted contact lenses Wöhlk SPORT CONTRAST on color discrimination was investigated with the Erlangen colour measurement system in a study with 14 and 16 subjects, respectively. In relation to a yellow reference color located at u' = 0.2487/v' = 0.5433, measurements of color discrimination thresholds were taken in up to 6 different color coordinate axes. Based on these thresholds, color discrimination ellipses were calculated. These results are given in the Derrington, Krauskopf and Lennie (DKL) color system. Both contact lenses caused a shift of the reference color towards higher saturated colors. Color discrimination ability with the yellow and orange colored lenses was significantly enhanced along the blue-yellow axis in comparison to the reference measurements without a tinted filter. Along the red-green axis only the orange lens caused a significant reduction of color discrimination threshold distance to the reference color. Yellow and orange tinted contact lenses enhance the ability of color discrimination. If the transmission spectra and the induced changes are taken into account, these results can also be applied to other filter media, such as blue filter intraocular lenses.
Minimizing yellow-bellied sapsucker damage

Treesearch

Gayne G. Erdmann; Ralph M., Jr. Peterson

1992-01-01

The yellow-bellied sapsucker is a migratory woodpecker that feeds on a wide variety of orchard, shade, and forest trees. Instead of drilling holes to find insects like other woodpeckers, sapsuckers drill holes in living trees to feed on sap and phloem tissues. Yellow and paper birches are their favorite summer food sources on their nesting grounds in Upper Michigan and...
Silvical Characteristics of Yellow-Poplar

Treesearch

David F. Olson

1969-01-01

Yellow-poplar (Liriorlentlron tulipifera L.) is also commonly known as tulip poplar, tulip tree, white-poplar, whitewood, and "poplar" (60). It gets its name from the tulip-like flowers which it bears in the late spring. Because of the excellent form and rapid growth of the tree, plus the fine working qualities of the wood, yellow-poplar is one of the most...
Yellow fever disease: density equalizing mapping and gender analysis of international research output

PubMed Central

2013-01-01

Background A number of scientific papers on yellow fever have been published but no broad scientometric analysis on the published research of yellow fever has been reported. The aim of the article based study was to provide an in-depth evaluation of the yellow fever field using large-scale data analysis and employment of bibliometric indicators of production and quantity. Methods Data were retrieved from the Web of Science database (WoS) and analyzed as part of the NewQis platform. Then data were extracted from each file, transferred to databases and visualized as diagrams. Partially by means of density-equalizing mapping makes the findings clear and emphasizes the output of the analysis. Results In the study period from 1900 to 2012 a total of 5,053 yellow fever-associated items were published by 79 countries. The United States (USA) having the highest publication rate at 42% (n = 751) followed by far from Brazil (n = 203), France (n = 149) and the United Kingdom (n = 113). The most productive journals are the “Public Health Reports”, the “American Journal of Tropical Medicine and Hygiene” and the “Journal of Virology”. The gender analysis showed an overall steady increase of female authorship from 1950 to 2011. Brazil is the only country of the five most productive countries with a higher proportion of female scientists. Conclusions The present data shows an increase in research productivity over the entire study period, in particular an increase of female scientists. Brazil shows a majority of female authors, a fact that is confirmed by other studies. PMID:24245856
Dermatology Internet Yellow Page advertising.

PubMed

Francis, Shayla; Kozak, Katarzyna Z; Heilig, Lauren; Lundahl, Kristy; Bowland, Terri; Hester, Eric; Best, Arthur; Dellavalle, Robert P

2006-07-01

Patients may use Internet Yellow Pages to help select a physician. We sought to describe dermatology Internet Yellow Page advertising. Dermatology advertisements in Colorado, California, New York, and Texas at 3 Yellow Page World Wide Web sites were systematically examined. Most advertisements (76%; 223/292) listed only one provider, 56 listed more than one provider, and 13 listed no practitioner names. Five advertisements listed provider names without any credentialing letters, 265 listed at least one doctor of medicine or osteopathy, and 9 listed only providers with other credentials (6 doctors of podiatric medicine and 3 registered nurses). Most advertisements (61%; 179/292) listed a doctor of medicine or osteopathy claiming board certification, 78% (139/179) in dermatology and 22% (40/179) in other medical specialties. Four (1%; 4/292) claims of board certification could not be verified (one each in dermatology, family practice, dermatologic/cosmetologic surgery, and laser surgery). Board certification could be verified for most doctors of medicine and osteopathy not advertising claims of board certification (68%; 41/60; 32 dermatology, 9 other specialties). A total of 50 advertisements (17%) contained unverifiable or no board certification information, and 47 (16%) listed a physician with verifiable board certification in a field other than dermatology. All Internet Yellow Page World Wide Web sites and all US states were not examined. Nonphysicians, physicians board certified in medical specialties other than dermatology, and individuals without verifiable board certification in any medical specialty are advertising in dermatology Internet Yellow Pages. Many board-certified dermatologists are not advertising this certification.
21 CFR 573.1020 - Yellow prussiate of soda.

Code of Federal Regulations, 2010 CFR

2010-04-01

...) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS FOOD ADDITIVES PERMITTED IN FEED AND DRINKING WATER OF ANIMALS Food Additive Listing § 573.1020 Yellow prussiate of soda. Yellow prussiate of soda (sodium... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Yellow prussiate of soda. 573.1020 Section 573...
21 CFR 573.1020 - Yellow prussiate of soda.

Code of Federal Regulations, 2012 CFR

2012-04-01

...) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS FOOD ADDITIVES PERMITTED IN FEED AND DRINKING WATER OF ANIMALS Food Additive Listing § 573.1020 Yellow prussiate of soda. Yellow prussiate of soda (sodium... 21 Food and Drugs 6 2012-04-01 2012-04-01 false Yellow prussiate of soda. 573.1020 Section 573...
21 CFR 573.1020 - Yellow prussiate of soda.

Code of Federal Regulations, 2014 CFR

2014-04-01

...) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS FOOD ADDITIVES PERMITTED IN FEED AND DRINKING WATER OF ANIMALS Food Additive Listing § 573.1020 Yellow prussiate of soda. Yellow prussiate of soda (sodium... 21 Food and Drugs 6 2014-04-01 2014-04-01 false Yellow prussiate of soda. 573.1020 Section 573...
21 CFR 573.1020 - Yellow prussiate of soda.

Code of Federal Regulations, 2013 CFR

2013-04-01

...) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS FOOD ADDITIVES PERMITTED IN FEED AND DRINKING WATER OF ANIMALS Food Additive Listing § 573.1020 Yellow prussiate of soda. Yellow prussiate of soda (sodium... 21 Food and Drugs 6 2013-04-01 2013-04-01 false Yellow prussiate of soda. 573.1020 Section 573...
21 CFR 573.1020 - Yellow prussiate of soda.

Code of Federal Regulations, 2011 CFR

2011-04-01

...) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS FOOD ADDITIVES PERMITTED IN FEED AND DRINKING WATER OF ANIMALS Food Additive Listing § 573.1020 Yellow prussiate of soda. Yellow prussiate of soda (sodium... 21 Food and Drugs 6 2011-04-01 2011-04-01 false Yellow prussiate of soda. 573.1020 Section 573...

Temperature induced variation in gene expression of thyroid hormone receptors and deiodinases of European eel (Anguilla anguilla) larvae.

PubMed

Politis, S N; Servili, A; Mazurais, D; Zambonino-Infante, J-L; Miest, J J; Tomkiewicz, J; Butts, I A E

2018-04-01

Thyroid hormones (THs) are key regulators of growth, development, and metabolism in vertebrates and influence early life development of fish. TH is produced in the thyroid gland (or thyroid follicles) mainly as T4 (thyroxine), which is metabolized to T3 (3,5,3'-triiodothyronine) and T2 (3,5-diiodothyronine) by deiodinase (DIO) enzymes in peripheral tissues. The action of these hormones is mostly exerted by binding to a specific nuclear thyroid hormone receptor (THR). In this study, we i) cloned and characterized thr sequences, ii) investigated the expression pattern of the different subtypes of thrs and dios, and iii) studied how temperature affects the expression of those genes in artificially produced early life history stages of European eel (Anguilla anguilla), reared in different thermal regimes (16, 18, 20 and 22 °C) from hatch until first-feeding. We identified 2 subtypes of thr (thrα and thrβ) with 2 isoforms each (thrαA, thrαB, thrβA, thrβB) and 3 subtypes of deiodinases (dio1, dio2, dio3). All thr genes identified showed high similarity to the closely related Japanese eel (Anguilla japonica). We found that all genes investigated in this study were affected by larval age (in real time or at specific developmental stages), temperature, and/or their interaction. More specifically, the warmer the temperature the earlier the expression response of a specific target gene. In real time, the expression profiles appeared very similar and only shifted with temperature. In developmental time, gene expression of all genes differed across selected developmental stages, such as at hatch, during teeth formation or at first-feeding. Thus, we demonstrate that thrs and dios show sensitivity to temperature and are involved in and during early life development of European eel. Copyright © 2017 Elsevier Inc. All rights reserved.
Influence of the Yellow Sea Warm Current on phytoplankton community in the central Yellow Sea

NASA Astrophysics Data System (ADS)

Liu, Xin; Chiang, Kuo-Ping; Liu, Su-Mei; Wei, Hao; Zhao, Yuan; Huang, Bang-Qin

2015-12-01

In early spring, a hydrological front emerges in the central Yellow Sea, resulting from the intrusion of the high temperature and salinity Yellow Sea Warm Current (YSWC). The present study, applying phytoplankton pigments and flow cytometry measurements in March of 2007 and 2009, focuses on the biogeochemical effects of the YSWC. The nutrients fronts were coincident with the hydrological front, and a positive linear relationship between nitrate and salinity was found in the frontal area. This contrast with the common situation of coastal waters where high salinity values usually correlate with poor nutrients. We suggested nutrient concentrations of the YSWC waters might have been enhanced by mixing with the local nutrient-rich waters when it invaded the Yellow Sea from the north of the Changjiang estuary. In addition, our results indicate that the relative abundance of diatoms ranged from 26% to 90%, showing a higher value in the YSCC than in YSWC waters. Similar distributions were found between diatoms and dinoflagellates, however the cyanobacteria and prasinophytes showed an opposite distribution pattern. Good correlations were found between the pigments and flow cytometry observations on the picophytoplankton groups. Prasinophytes might be the major contributor to pico-eukaryotes in the central Yellow Sea as similar distributional patterns and significant correlations between them. It seems that the front separates the YSWC from the coastal water, and different phytoplankton groups are transported in these water masses and follow their movement. These results imply that the YSWC plays important roles in the distribution of nutrients, phytoplankton biomass and also in the community structure of the central Yellow Sea.
Heavy Metals Uptake by Asian Swamp Eel, Monopterus albus from Paddy Fields of Kelantan, Peninsular Malaysia: Preliminary Study

PubMed Central

Yin, Sow Ai; Ismail, Ahmad; Zulkifli, Syaizwan Zahmir

2012-01-01

Swamp eel, Monopterus albus is one of the common fish in paddy fields, thus it is suitable to be a bio-monitor for heavy metals pollution studies in paddy fields. This study was conducted to assess heavy metals levels in swamp eels collected from paddy fields in Kelantan, Malaysia. The results showed zinc [Zn (86.40 μg/g dry weight)] was the highest accumulated metal in the kidney, liver, bone, gill, muscle and skin. Among the selected organs, gill had the highest concentrations of lead (Pb), cadmium (Cd) and nickel (Ni) whereas muscle showed the lowest total metal accumulation of Zn, Pb, copper (Cu), Cd and Ni. Based on the Malaysian Food Regulation, the levels of Zn and Cu in edible parts (muscle and skin) were within the safety limits. However, Cd, Pb and Ni exceeded the permissible limits. By comparing with the maximum level intake (MLI), Pb, Ni and Cd in edible parts can still be consumed. This investigation indicated that M. albus from paddy fields of Kelantan are safe for human consumption with little precaution. PMID:24575231
49 CFR 173.188 - White or yellow phosphorus.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 2 2010-10-01 2010-10-01 false White or yellow phosphorus. 173.188 Section 173... Class 7 § 173.188 White or yellow phosphorus. Phosphorus, white or yellow, when offered for... pound) of phosphorus with screw-top closures; or (2) Steel drums (1A1) not over 250 L (66 gallons...
49 CFR 173.188 - White or yellow phosphorus.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 2 2011-10-01 2011-10-01 false White or yellow phosphorus. 173.188 Section 173... Class 7 § 173.188 White or yellow phosphorus. Phosphorus, white or yellow, when offered for... pound) of phosphorus with screw-top closures; or (2) Steel drums (1A1) not over 250 L (66 gallons...
Decomposition of L a2 -xS rxCu O4 into several L a2O3 phases at elevated temperatures in ultrahigh vacuum inside a transmission electron microscope

NASA Astrophysics Data System (ADS)

Jeong, Jong Seok; Wu, Wangzhou; Topsakal, Mehmet; Yu, Guichuan; Sasagawa, Takao; Greven, Martin; Mkhoyan, K. Andre

2018-05-01

We report the decomposition of L a2 -xS rxCu O4 into L a2O3 and Cu nanoparticles in ultrahigh vacuum, observed by in situ heating experiments in a transmission electron microscope. The analysis of electron diffraction data reveals that the phase decomposition process starts at about 150 °C and is considerably expedited in the temperature range of 350 °C-450 °C. Two major resultant solid phases are identified as metallic Cu and L a2O3 by electron diffraction, simulation, and electron energy-loss spectroscopy (EELS) analyses. With the aid of calculations, L a2O3 phases are further identified to be derivatives of a fluorite structure—fluorite, pyrochlore, and (distorted) bixbyite—characterized by different oxygen-vacancy order. Additionally, the bulk plasmon energy and the fine structures of the O K and La M4 ,5 EELS edges are reported for these structures, along with simulated O K x-ray absorption near-edge structure. The resultant Cu nanoparticles and L a2O3 phases remain unchanged after cooling to room temperature.
21 CFR 137.280 - Bolted yellow corn meal.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Bolted yellow corn meal. 137.280 Section 137.280... Flours and Related Products § 137.280 Bolted yellow corn meal. Bolted yellow corn meal conforms to the definition and standard of identity prescribed by § 137.255 for bolted white corn meal except that cleaned...
21 CFR 137.280 - Bolted yellow corn meal.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Bolted yellow corn meal. 137.280 Section 137.280... Flours and Related Products § 137.280 Bolted yellow corn meal. Bolted yellow corn meal conforms to the definition and standard of identity prescribed by § 137.255 for bolted white corn meal except that cleaned...
The nervus terminalis ganglion in Anguilla rostrata: an immunocytochemical and HRP histochemical analysis.

PubMed

Grober, M S; Bass, A H; Burd, G; Marchaterre, M A; Segil, N; Scholz, K; Hodgson, T

1987-12-08

Immunocytochemistry and retrograde horseradish peroxidase (HRP) transport were used to study the ganglion of the nervus terminalis in the American eel, Anguilla rostrata. Luteinizing hormone releasing hormone (LHRH) like immunoreactivity was found in large, ganglion-like cells located ventromedially at the junction of the telencephalon and olfactory bulb and in fibers within the retina and olfactory epithelium. HRP transport from the retina demonstrated direct connections with both the ipsi- and contralateral populations of these ganglion-like cells. Given the well-documented role of both olfaction and vision during migratory and reproductive phases of the life cycle of eels, the robust nature of a nervus terminalis system in these fish may present a unique opportunity to study the behavioral correlates of structure-function organization in a discrete population of ganglion-like cells.
Characterization of organochlorine pesticides, brominated flame retardants and dioxin-like compounds in shellfish and eel from Fiji.

PubMed

Lal, Vincent; Bridgen, Phil; Votadroka, Waisea; Raju, Rupantri; Aalbersberg, William

2014-09-01

This article gives an overview of a range of persistent organic pollutant chemical levels in shellfish (Batissa violacea and Anadara antiquata) species and eel (Gymnothorax flavimarginatus) from Fiji. As there is limited data in published literature to date, this paper reports first data on a range of persistent organic pollutants and highlights the more prominent POP chemicals present in marine biota in Fiji. A significant number of POP chemicals were detected (e.g. 17 PCDD/PCDF, 12dl-PCBs, organochlorine pesticides and brominated flame retardants), the concentrations found were generally low (e.g. parts per billion level). The low levels of contamination are indicative of a low input from long range and short-range transport as well as few local point sources. Also concentrations of POPs in eel and shellfish from Fiji are low in comparison to wild species in other regions and are within acceptable limits for POP chemicals in fish and fishery products set by the European Union. It describes also results of early studies on basic POPs levels in shellfish in several Pacific Island Countries, which generally show relatively low levels. Copyright © 2014 Elsevier B.V. All rights reserved.
Status and nesting of the yellow-billed cuckoo in Puerto Rico

USGS Publications Warehouse

Kepler, Cameron B.; Kepler, Angela K.

1978-01-01

Knowledge of the status of the Yellow-billed Cuckoo (Coccyzus americanus) in the Greater Antilles has been clouded by the bird's elusive behavior and spotty distribution on the islands, and by a major movement of North American migrants through the area to and from their wintering grounds in South America. Although Bond (1956) states that they breed in the Greater Antilles, nests are known only from Cuba, St. Croix (Bond 1956), and Jamaica (Lack 1976), and Bond considered the bird rare in Puerto Rico. Other students of Puerto Rican ornithology have suggested that the species is "uncommon" (Leopold 1963), "rare" (McCandless 1958), or a regular migrant or resident species (Wetmore 1916). Although no nests have been reported, most authors suspect that it breeds, and agree that it frequents coastal areas in western or southwestern Puerto Rico, primarily from May to September (Wetmore 1916, Danforth 1936, McCandless 1958, Leopold 1963, Biaggi 1970). From 1968 to 1971 we conducted linear strip censuses along pre-selected routes throughout the island, counting all birds heard or seen (Kepler and Kepler 1970); data we obtained on the Yellow-billed Cuckoo helped clarify and amplify existing information on its distribution and status.
Effects of countdown timers on driver behavior after the yellow onset at Chinese intersections.

PubMed

Long, Kejun; Han, Lee D; Yang, Qiang

2011-10-01

Few studies have focused on the effect of countdown timers at signalized intersections in China, where such timers are widely deployed for their perceived benefits of increased safety and capacity. This study examines the effect of countdown timers on driver behavior during the yellow interval. Signal phasing and traffic operations were videotaped at 4 comparable signalized intersections under normal conditions. Microscopic details were extracted manually at 25 Hz to yield 24 h of data on onset time of the yellow, onset time of the red, driver location and actions after the onset of the yellow, red light-running violations, etc. For comparable intersections with and without countdown timers, driver behavior measured by driver decision (stop or go) and vehicle entry time (when the vehicle crosses the stop line) were analyzed using binary logistical regression (BLR) and a nonparametric test, respectively. The results suggest that countdown timers can indeed influence driver behaviors, in terms of decisions to stop or cross the intersection as well as the distribution of vehicle entry times. There was a strong correlation between the presence of countdown timers and an increase in red light violations. Countdown timers may lead to increased entrance into the intersection during the later portions of the yellow and even the red. This alarming finding calls for further research as well as for serious consideration before the field deployment of countdown timers.
Yellow Fever Outbreak, Southern Sudan, 2003

PubMed Central

Onyango, Clayton O.; Grobbelaar, Antoinette A.; Gibson, Georgina V.F.; Sang, Rosemary C.; Sow, Abdourahmane; Swanepoel, Robert

2004-01-01

In May 2003, an outbreak of fatal hemorrhagic fever, caused by yellow fever virus, occurred in southern Sudan. Phylogenetic analysis showed that the virus belonged to the East African genotype, which supports the contention that yellow fever is endemic in East Africa with the potential to cause large outbreaks in humans. PMID:15498174
Various Measures of the Effectiveness of Yellow Goggles

DTIC Science & Technology

1980-10-08

technique which is widely used r.o improve vision under these conditions is the use of yellow goggles. Skiers commonly don yellow goggles...different laboratory studies are presented. Two of the studies were of depth perception, since skiers believe that yellow goggles help them...selected for measurement because of practical considerations and theoretical implications. EXPERIMENTS ON DEPTH PERCEPTION Background Since skiers
The experiments of Ramón M. Termeyer SJ on the electric eel in the River Plate region (c. 1760) and other early accounts of Electrophorus electricus.

PubMed

de Asúa, Miguel

2008-01-01

This paper focuses on Ramón M. Termeyer SJ (1737-1814?), a naturalist who experimented with the electric eel in the River Plate region during the 1760s. After going through an enumeration of the chroniclers that since the sixteenth century noticed the benumbing discharge of Electrophorus electricus, the article summarizes the work that immediately preceded Termeyer's and considers as a term of comparison the experiments on the electric eel performed by Bertrand Bajon (fl. 1751-1778) in the French Guyanne. It ends by discussing the meaning of Termeyer's 1781 and 1810 articles in the light of contemporary ideas of animal electricity.
Hydrogen ion titrations of the anodic and cathodic haemoglobin components of the European eel Anguilla anguilla.

PubMed

Brauner, C J; Weber, R E

1998-09-01

H+ titrations were conducted on the separated haemoglobin components of eel Anguilla anguilla in both the oxygenated and deoxygenated states. In anodic haemoglobin, the addition of GTP, and to a lesser extent C1-, increased the magnitude of the Haldane effect and shifted its maximum value into the in vivo pH range. Of the 22 histidine residues in the anodic component, only approximately seven were titratable, presumably the beta-chain residues at positions 41, 97, 109 and 146 (helical positions C7, FG4, G11 and HC3, respectively). In cathodic haemoglobin, a small negative Haldane effect was observed at pH values between 6.8 and 8.5 which disappeared in the presence of GTP (molar ratio 3:1 GTP:haemoglobin tetramer). GTP had virtually no effect on the buffer value at fixed oxygenation status, and the lowest buffer value was observed at in vivo pH values. No titratable histidine residues were observed in the cathodic component, indicating that all 14 histidines in this component are buried. We conclude that the anodic component, which constitutes two-thirds of the haemoglobin in the eel, plays the predominant role in CO2 transport and pH homeostasis in vivo.
Cryopreservation of green fluorescent protein (GFP)-labeled primordial germ cells with GFP fused to the 3' untranslated region of the nanos gene by vitrification of Japanese eel (Anguilla japonica) somite stage embryos.

PubMed

Kawakami, Y; Ishihara, M; Saito, T; Fujimoto, T; Adachi, S; Arai, K; Yamaha, E

2012-12-01

Primordial germ cells (PGC) are the only cell type in developing embryos with the potential to transmit genetic information to the next generation. In this study, PGC of Japanese eel (Anguilla japonica) were visualized by injection of mRNA synthesized from a construct carrying the green fluorescent protein (GFP) gene fused to the 3' untranslated region of the Japanese eel nanos gene. We investigated the feasibility of cryopreserving Japanese eel PGC by vitrification of dechorionated whole somite stage embryos. The GFP-labeled PGC were rapidly cooled using liquid nitrogen after exposure to a pretreatment solution containing 1.5 M cryoprotectant (methanol, dimethyl sulfoxide, and glycerol for 10 min and ethylene glycol for 10, 20, and 30 min) and a vitrification solution containing 3 M cryoprotectant and 0.5 M sucrose for 1, 5, and 10 min. Ethylene glycerol is an effective cryoprotectant for embryonic cells and shows no evidence of ice formation after thawing. Vitrified and thawed PGC were transplanted into blastula stage embryos from zebrafish (Danio rerio). The GFP-labeled PGC migrated toward the host gonadal ridge, suggesting maintenance of their normal migration motility. These techniques may assist in achieving inter- and intraspecies germ-line chimers using donor Japanese eel PGC.
New host record of avian tuberculosis in an American white pelican, Pelecanus erythrorhynchos

USGS Publications Warehouse

Nol, P.; Brannian, R.E.; Berlowski, B.M.; Wolcott, M.J.; Rocke, T.E.

2003-01-01

An American white pelican (P. erythrorhyncos) was captured in August 1999 in California, USA. The bird was unable to fly away or evade the boat, and showed considerable aggression when captured. Numerous multifocal, 1 mm diameter, yellow masses were observed throughout the lungs and air sacs. The liver was yellow. A 6 cm diameter, firm spherical mass was present in the abdominal cavity, distal to the liver. Aspergillus spp. were cultured from the air sac tissue. Mycobacterium avium was identified from the splenic mass.
Estimates of suspended-sediment flux and bedform activity on the inner portion of the Eel continental shelf

USGS Publications Warehouse

Cacchione, D.A.; Wiberg, P.L.; Lynch, J.; Irish, J.; Traykovski, P.

1999-01-01

Energetic waves, strong bottom currents, and relatively high rates of sediment discharge from the Eel River combined to produce large amounts of suspended-sediment transport on the inner continental shelf near the Eel River during the winter of 1995-1996. Bottom-boundary-layer (BBL) measurements at a depth of ~50 m using the GEOPROBE tripod showed that the strongest near-bottom flows (combined wave and current speeds of over 1 m/s) and highest sediment concentrations (exceeding 2 g/l at ~1.2 m above the bed) occurred during two storms, one in December 1995 and the other in February 1996. Discharge from the Eel River during these storms was estimated at between 2 and 4 x 103 m3/s. Suspended-sediment flux (SSF) was measured 1.2 m above the bed and calculated throughout the BBL, by applying the tripod data to a shelf sediment-transport model. These results showed initially northward along-shelf SSF during the storms, followed by abrupt and persistent southward reversals. Along-shelf flux was more pronounced during and after the December storm than in February. Across-shelf SSF over the entire measurement period was decidedly seaward. This seaward transport could be responsible for surficial deposits of recent sediment on the outer shelf and upper continental slope in this region. Sediment ripples and larger bedforms were observed in the very fine to fine sand at 50-m depth using a sector-scanning sonar mounted on the tripod. Ripple wavelengths estimated from the sonar images were about 9 cm, which compared favorably with photographs of the bottom taken with a camera mounted on the tripod. The ripple patterns were stable during periods of low combined wave-current bottom stresses, but changed significantly during high-stress events, such as the February storm. Two different sonic altimeters recorded changes in bed elevation of 10 to 20 cm during the periods of measurement. These changes are thought to have been caused principally by the migration of low-amplitude, long
Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel)

PubMed Central

Stasiuk, Maria; Janiszewska, Alicja; Kozubek, Arkadiusz

2014-01-01

Phenolic lipids were isolated from rye grains, cashew nutshell liquid (CNSL) from Anacardium occidentale, and fruit bodies of Merrulius tremellosus, and their effects on the electric eel acetylcholinesterase activity and conformation were studied. The observed effect distinctly depended on the chemical structure of the phenolic lipids that were available for interaction with the enzyme. All of the tested compounds reduced the activity of acetylcholinesterase. The degree of inhibition varied, showing a correlation with changes in the conformation of the enzyme tested by the intrinsic fluorescence of the Trp residues of the protein. PMID:24787269

Trust in the patient’s history: cough and eels

PubMed Central

Mackay, Alexander John; Burke, Stephen; Bothamley, Graham

2009-01-01

A 54-year-old ex-smoker was referred to the respiratory clinic for breathlessness and wheeze. His symptoms started while eating jellied eels when he felt something stick in his throat. The following day, his general practitioner heard a wheeze in his right lower chest. A chest radiograph noted no foreign body. A course of antibiotics did not help, but steroids were beneficial. On clinic review the examination was normal apart from enlarged and inflamed tonsils. Fibreoptic bronchoscopy revealed a suspicious looking mass lesion completely occluding the bronchus intermedius. Biopsies of the lesion revealed inflamed bronchial mucosa and a fragment of bone. There was no evidence of dysplasia or malignancy. A computed tomography scan of the thorax revealed a 5 mm endobronchial soft tissue density with areas of calcification within the bronchus intermedius. At rigid bronchoscopy a spiny bone was removed and subsequently the patient’s shortness of breath, cough and wheeze improved. PMID:22110553
Dimension yields from yellow-poplar lumber

Treesearch

R. C. Gilmore; J. D. Danielson

1984-01-01

The available supply of yellow poplar (Liriodendron tulipifera L.), its potential for new uses, and its continuing importance to the furniture industry have created a need to accumulate additional information about this species. As an aid to better utilization of this species, charts for determining cutting stock yields from yellow poplar lumber are presented for each...
A PDS Partnership Goes International: Phase I of an American-Slovenian Collaborative Research Project

ERIC Educational Resources Information Center

Catelli, Linda; Carlino, Joan; Jackson, Valerie; Petraglia, GinaMarie

2011-01-01

The focus of the article is on Phase I of an American-Slovenian collaborative research project that was aimed at investigating effective classroom teaching-learning performances. The international project involved Dowling College and its first Professional Development School (PDS)--the Belmont Elementary PDS--and the University of Primorska at…
The complete nucleotide sequence of the Barley yellow dwarf virus-RMV genome reveals it to be a new Polerovirus distantly related to other yellow dwarf viruses

USDA-ARS?s Scientific Manuscript database

The yellow dwarf viruses (YDVs) of the Luteoviridae family represent the most widespread group of cereal viruses worldwide. They include the Barley yellow dwarf viruses (BYDVs) of genus Luteovirus, the Cereal yellow dwarf viruses (CYDVs) and Wheat yellow dwarf virus (WYDV) of genus Polerovirus. All ...
Effects of deep frying on proximate composition and micronutrient of Indian mackerel (Rastrelliger kanagurta), eel (Monopterus albus) and cockle (Anadara granosa).

PubMed

Rahman, M M; Zamri, M; Fadilla, N

2012-06-15

This study was conducted to determine the proximate composition and four micronutrients (Cd, Cu, Mn and Zn) of Indian Mackerel (Rastrelliger kanagurta), Eel (Monopterus albus) and Cockle (Anadara granosa). All fish and shellfish were purchased from local fish market in Kuantan city. All samples of each species were mixed and divided into two groups based on random selection. Each group were again divided into 3 sub-groups which were considered as replications. The first group were kept uncooked. The second group were fried in a beaker of 400 mL palm cooking oil capacity at a temperature approximately of 180 degrees C for a 15 min period. Both raw and fried samples were analysed following standard methods to determine protein, lipid, ash, moisture, carbohydrate, Cd, Cu, Mn and Zn contents. Results showed that protein content was higher in Indian mackerel and eel than cockle while overall Cd, Cu, Mn and Zn contents were higher in cockle than Indian mackerel and eel. Therefore, fish is better than shellfish in the nutritional point of view. Fried fish and shellfish had very high fat content. Therefore, frying cannot be recommended to prepare a healthy diet. More research is needed including all cooking methods of fish to know the nutritional changes by each cooking method. Fish contains many important fatty acids and amino acids which might be lost during frying. Therefore, future study should include the effects of different cooking methods on amino acids and fatty acids compositions of fish and shellfish.
7 CFR 28.441 - Strict Middling Yellow Stained Color.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 2 2014-01-01 2014-01-01 false Strict Middling Yellow Stained Color. 28.441 Section... Strict Middling Yellow Stained Color. Strict Middling Yellow Stained Color is color which is deeper than that of Strict Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] ...
7 CFR 28.441 - Strict Middling Yellow Stained Color.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 2 2013-01-01 2013-01-01 false Strict Middling Yellow Stained Color. 28.441 Section... Strict Middling Yellow Stained Color. Strict Middling Yellow Stained Color is color which is deeper than that of Strict Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] ...
7 CFR 28.441 - Strict Middling Yellow Stained Color.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 2 2012-01-01 2012-01-01 false Strict Middling Yellow Stained Color. 28.441 Section... Strict Middling Yellow Stained Color. Strict Middling Yellow Stained Color is color which is deeper than that of Strict Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] ...
7 CFR 28.441 - Strict Middling Yellow Stained Color.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 2 2011-01-01 2011-01-01 false Strict Middling Yellow Stained Color. 28.441 Section... Strict Middling Yellow Stained Color. Strict Middling Yellow Stained Color is color which is deeper than that of Strict Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] ...
7 CFR 28.441 - Strict Middling Yellow Stained Color.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 2 2010-01-01 2010-01-01 false Strict Middling Yellow Stained Color. 28.441 Section... Strict Middling Yellow Stained Color. Strict Middling Yellow Stained Color is color which is deeper than that of Strict Middling Tinged Color. [57 FR 34498, Aug. 5, 1992] ...
Dietary calcein marking of brook trout, Atlantic salmon, yellow perch, and coho salmon scales

USGS Publications Warehouse

Honeyfield, D.C.; Ostrowski, C.S.; Fletcher, J.W.; Mohler, J.W.

2006-01-01

Brook trout Salvelinus fontinalis, Atlantic salmon Salmo salar, coho salmon Oncorhynchus kisutch, and yellow perch Perca flavescens fed calcein for 5 d showed characteristic calcein scale marks 7-10 d postmarking. In fish fed 0.75 or 1.25 g of calcein per kilogram of feed, the percentage of fish that exhibited a calcein mark was 100% in brook trout, 93-98% in Atlantic salmon, 60% in yellow perch, and 0% in coho salmon. However, when coho salmon were fed 5.25 g calcein/kg feed, 100% marking was observed 7-10 d postmarking. Brook trout were successfully marked twice with distinct bands when fed calcein 5 months apart. Brook trout scale pixel luminosity increased as dietary calcein increased in experiment 2. For the second calcein mark, scale pixel luminosity from brook trout fed 1.25 g calcein/kg feed was numerically higher (P < 0.08) than scales from fish fed 0.75 g calcein/kg feed. Mean pixel luminosity of calcein-marked Atlantic salmon scales was 57.7 for fish fed 0.75 g calcein/kg feed and 55.2 for fish fed 1.25 g calcein/kg feed. Although feed acceptance presented a problem in yellow perch, these experiments provide evidence that dietary calcein is a viable tool for marking fish for stock identification. ?? Copyright by the American Fisheries Society 2006.
Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution.

PubMed

Carda-Diéguez, Miguel; Ghai, Rohit; Rodríguez-Valera, Francisco; Amaro, Carmen

2017-12-21

Fish skin mucosal surfaces (SMS) are quite similar in composition and function to some mammalian MS and, in consequence, could constitute an adequate niche for the evolution of mucosal aquatic pathogens in natural environments. We aimed to test this hypothesis by searching for metagenomic and genomic evidences in the SMS-microbiome of a model fish species (Anguilla Anguilla or eel), from different ecosystems (four natural environments of different water salinity and one eel farm) as well as the water microbiome (W-microbiome) surrounding the host. Remarkably, potentially pathogenic Vibrio monopolized wild eel SMS-microbiome from natural ecosystems, Vibrio anguillarum/Vibrio vulnificus and Vibrio cholerae/Vibrio metoecus being the most abundant ones in SMS from estuary and lake, respectively. Functions encoded in the SMS-microbiome differed significantly from those in the W-microbiome and allowed us to predict that successful mucus colonizers should have specific genes for (i) attachment (mainly by forming biofilms), (ii) bacterial competence and communication, and (iii) resistance to mucosal innate immunity, predators (amoeba), and heavy metals/drugs. In addition, we found several mobile genetic elements (mainly integrative conjugative elements) as well as a series of evidences suggesting that bacteria exchange DNA in SMS. Further, we isolated and sequenced a V. metoecus strain from SMS. This isolate shares pathogenicity islands with V. cholerae O1 from intestinal infections that are absent in the rest of sequenced V. metoecus strains, all of them from water and extra-intestinal infections. We have obtained metagenomic and genomic evidence in favor of the hypothesis on the role of fish mucosal surfaces as a specialized habitat selecting microbes capable of colonizing and persisting on other comparable mucosal surfaces, e.g., the human intestine.
Characterization of Nb Superconducting Radio Frequency Cavities Based On In-Situ STEM And EELS

NASA Astrophysics Data System (ADS)

Tao, Runzhe

Niobium, a 4d transition metal, has the highest superconducting transition temperature (Tc=9.2K) of any elemental superconductor as type II superconductor with coherent length, sigma approximately that of the penetration length, lambda. Pure niobium is grey in color and very soft, which makes this metal easily fabricable into different shapes for superconducting radio- frequency (SRF) cavities. Such cavities are used in some modern accelerators (SNS, CEBAF, XFEL), and are intended for usage in the next generation of particle accelerators, such as ILC. Since the crucial part of the cavities is top 100 nm of Nb near the inner cavity surface, considering the penetration depth is around 40 nm, it has attracted more and more attention in improving the surface process for optimizing the performance of the cavities. Nowadays, the main treatment of the Nb surface includes electro polishing (EP), buffered chemical polishing (BCP), high temperature baking (800 °C, 1000 °C and 1200 °C) and mild baking (120 °C). Firstly, the two half cells are welded together and the weld line is quite rough; there exists a lot of visible pits and defects on the inner shell of cavities. In this Ph.D. thesis, novel techniques in a scanning transmission electron microscope (STEM) that can be used to analyze the atomic scale structure-property relationship, both at room tem- perature and high/LN 2 temperature, are explored. Specifically, by using correlated Z-contrast imaging and electron energy loss spectrum (EELS), the structure, composition and bonding can be characterized directly on the atomic scale, also, light atoms, like H, O and C, are visible in ABF images. For the examining the defect behavior on the cavity surface, heating and cold stages are involved to simulate the baking treatment and low-temperature environments. These studies will serve as an important reference for qualifying different surface treatments to further improve SRF cavities' performance. The experimental results
THE TRANSMISSION OF YELLOW FEVER

PubMed Central

Davis, Nelson C.

1930-01-01

1. Saimiri sciureus has been infected with yellow fever virus, both by the inoculation of infectious blood and by the bites of infective mosquitoes. Some of the monkeys have died, showing lesions, including hepatic necrosis, suggesting yellow fever as seen in human beings and in rhesus monkeys. Virus has been transferred back to M. rhesus from infected Saimiri both by blood inoculation and by mosquito bites. The virus undoubtedly has been maintained through four direct passages in Saimiri. Reinoculations of infectious material into recovered monkeys have not given rise to invasion of the blood stream by virus. Sera from recovered animals have protected M. rhesus against the inoculation of virus. 2. It has been possible to pass the virus to and from Ateleus ater by the injection of blood or liver and by the bites of mosquitoes. The livers from two infected animals have shown no necrosis. The serum from one recovered monkey proved to be protective for M. rhesus. 3. Only three out of twelve Lagothrix lagotricha have reacted to yellow fever virus by a rise in temperature. Probably none have died as a result of the infection. In only one instance has the virus been transferred back to M. rhesus. The sera of recovered animals have had a protective action against yellow fever virus. PMID:19869721
Lake Erie Yellow perch age estimation based on three structures: Precision, processing times, and management implications

USGS Publications Warehouse

Vandergoot, C.S.; Bur, M.T.; Powell, K.A.

2008-01-01

Yellow perch Perca flavescens support economically important recreational and commercial fisheries in Lake Erie and are intensively managed. Age estimation represents an integral component in the management of Lake Erie yellow perch stocks, as age-structured population models are used to set safe harvest levels on an annual basis. We compared the precision associated with yellow perch (N = 251) age estimates from scales, sagittal otoliths, and anal spine sections and evaluated the time required to process and estimate age from each structure. Three readers of varying experience estimated ages. The precision (mean coefficient of variation) of estimates among readers was 1% for sagittal otoliths, 5-6% for anal spines, and 11-13% for scales. Agreement rates among readers were 94-95% for otoliths, 71-76% for anal spines, and 45-50% for scales. Systematic age estimation differences were evident among scale and anal spine readers; less-experienced readers tended to underestimate ages of yellow perch older than age 4 relative to estimates made by an experienced reader. Mean scale age tended to underestimate ages of age-6 and older fish relative to otolith ages estimated by an experienced reader. Total annual mortality estimates based on scale ages were 20% higher than those based on otolith ages; mortality estimates based on anal spine ages were 4% higher than those based on otolith ages. Otoliths required more removal and preparation time than scales and anal spines, but age estimation time was substantially lower for otoliths than for the other two structures. We suggest the use of otoliths or anal spines for age estimation in yellow perch (regardless of length) from Lake Erie and other systems where precise age estimates are necessary, because age estimation errors resulting from the use of scales could generate incorrect management decisions. ?? Copyright by the American Fisheries Society 2008.
Yellow Fever Vaccine

MedlinePlus

... way to prevent yellow fever is to avoid mosquito bites by:staying in well-screened or air-conditioned areas, wearing clothes that cover most of your body, using an effective insect repellent, such as those containing DEET.
Preparative separation of two subsidiary colors of FD&C Yellow No. 5 (Tartrazine) using spiral high-speed counter-current chromatography◊

PubMed Central

Roque, Jose A.; Mazzola, Eugene P.; Ito, Yoichiro

2014-01-01

Specifications in the U.S. Code of Federal Regulations for the color additive FD&C Yellow No. 5 (Colour Index No. 19140) limit the level of the tetrasodium salt of 4-[(4',5-disulfo[1,1'-biphenyl]-2-yl)hydrazono]-4,5-dihydro-5-oxo-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylic acid and that of the trisodium salt of 4,4'-[4,5-dihydro-5-oxo-4-[(4-sulfophenyl)hydrazono]-1H-pyrazol-1,3-diyl]bis[benzenesulfonic acid], which are subsidiary colors abbreviated as Pk5 and Pk7, respectively. Small amounts of Pk5 and Pk7 are needed by the U.S. Food and Drug Administration for confirmatory analyses and for development of analytical methods. The present study describes the use of spiral high-speed counter-current chromatography (HSCCC) with the recently introduced highly polar organic/high-ionic strength aqueous solvent systems to separate Pk5 and Pk7 from a sample of FD&C Yellow No. 5 containing ~3.5% Pk5 and ~0.7% Pk7. Multiple ~1.0 g portions of FD&C Yellow No. 5 (totaling 6.4 g dye) were separated, using the upper phase of the solvent system 1-BuOH/EtOHabs/saturated ammonium sulfate/water, 1.7:0.3:1:1, v/v/v/v, as the mobile phase. After applying a specially developed method for removing the ammonium sulfate from the HSCCC-collected fractions, these separations resulted in an enriched mixture (~160 mg) of Pk5 and Pk7 (~46% and ~21%, respectively). Separation of the enriched mixture, this time using the lower phase of that solvent system as the mobile phase, resulted in ~ 61 mg of Pk5 collected in fractions whose purity ranged from 88.0% to 92.7% (by HPLC at 254 nm). Pk7 (20.7 mg, ~83% purity) was recovered from the upper phase of the column content. Application of this procedure also resulted in purifying the major component of FD&C Yellow No. 5 to >99% purity. The separated compounds were characterized by high-resolution mass spectrometry and several 1H and 13C nuclear magnetic resonance spectroscopic techniques (COSY, NOESY, HSQC, and HMBC). PMID:24755184
Yellow fever cases in Asia: primed for an epidemic.

PubMed

Wasserman, Sean; Tambyah, Paul Anantharajah; Lim, Poh Lian

2016-07-01

There is currently an emerging outbreak of yellow fever in Angola. Cases in infected travellers have been reported in a number of other African countries, as well as in China, representing the first ever documented cases of yellow fever in Asia. There is a large Chinese workforce in Angola, many of whom may be unvaccinated, increasing the risk of ongoing importation of yellow fever into Asia via busy commercial airline routes. Large parts of the region are hyperendemic for the related Flavivirus dengue and are widely infested by Aedes aegypti, the primary mosquito vector of urban yellow fever transmission. The combination of sustained introduction of viraemic travellers, an ecology conducive to local transmission, and an unimmunized population raises the possibility of a yellow fever epidemic in Asia. This represents a major global health threat, particularly in the context of a depleted emergency vaccine stockpile and untested surveillance systems in the region. In this review, the potential for a yellow fever outbreak in Asia is discussed with reference to the ecological and historical forces that have shaped global yellow fever epidemiology. The limitations of surveillance and vector control in the region are highlighted, and priorities for outbreak preparedness and response are suggested. Copyright © 2016 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Biology and biological control of Dalmatian and yellow toadflax

Treesearch

Sharlene E. Sing; Rosemarie De Clerck-Floate; Richard W. Hansen; Hal Pearce; Carol Bell Randall; Ivo Tosevski; Sarah M. Ward

2016-01-01

Dalmatian toadflax, Linaria dalmatica (L.) Mill., and yellow toadflax, Linaria vulgaris Mill., are exotic weeds of rangeland, grassland, forests, and cropland. Both Dalmatian and yellow toadflax are short-lived perennial forbs that are easily recognized by their yellow snapdragon- like flowers (Figure 1a, 1b). Both species propagate by seed and vegetatively...
33 CFR 117.225 - Yellow Mill Channel.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Yellow Mill Channel. 117.225 Section 117.225 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Connecticut § 117.225 Yellow Mill Channel. The...

The effects of hypoxic bradycardia and extracellular HCO3(-)/CO2 on hypoxic performance in the eel heart.

PubMed

Joyce, William; Simonsen, Maj; Gesser, Hans; Wang, Tobias

2016-02-01

During hypoxia, fishes exhibit a characteristic hypoxic bradycardia, the functional significance of which remains debated. Here, we investigated the hypothesis that hypoxic bradycardia primarily safeguards cardiac performance. In preparations from the European eel (Anguilla anguilla), a decrease in stimulation frequency from 40 to 15 beats min(-1), which replicates hypoxic bradycardia in vivo, vastly improved cardiac performance during hypoxia in vitro. As eels display dramatic shifts in extracellular HCO3(-)/CO2, we further investigated the effect this has upon hypoxic cardiac performance. Elevations from 10 mmol l(-1) HCO3(-)/1% CO2 to 40 mmol l(-1) HCO3(-)/4% CO2 had few effects on performance; however, further, but still physiologically relevant, increases to 70 mmol l(-1) HCO3(-)/7% CO2 compromised hypoxia tolerance. We revealed a four-way interaction between HCO3(-)/CO2, contraction frequency, hypoxia and performance over time, whereby the benefit of hypoxic bradycardia was most prolonged at 10 mmol l(-1) HCO3(-)/1% CO2. Together, our data suggest that hypoxic bradycardia greatly benefits cardiac performance, but its significance may be context specific. © 2016. Published by The Company of Biologists Ltd.
International travel between global urban centres vulnerable to yellow fever transmission.

PubMed

Brent, Shannon E; Watts, Alexander; Cetron, Martin; German, Matthew; Kraemer, Moritz Ug; Bogoch, Isaac I; Brady, Oliver J; Hay, Simon I; Creatore, Maria I; Khan, Kamran

2018-05-01

To examine the potential for international travel to spread yellow fever virus to cities around the world. We obtained data on the international flight itineraries of travellers who departed yellow fever-endemic areas of the world in 2016 for cities either where yellow fever was endemic or which were suitable for viral transmission. Using a global ecological model of dengue virus transmission, we predicted the suitability of cities in non-endemic areas for yellow fever transmission. We obtained information on national entry requirements for yellow fever vaccination at travellers' destination cities. In 2016, 45.2 million international air travellers departed from yellow fever-endemic areas of the world. Of 11.7 million travellers with destinations in 472 cities where yellow fever was not endemic but which were suitable for virus transmission, 7.7 million (65.7%) were not required to provide proof of vaccination upon arrival. Brazil, China, India, Mexico, Peru and the United States of America had the highest volumes of travellers arriving from yellow fever-endemic areas and the largest populations living in cities suitable for yellow fever transmission. Each year millions of travellers depart from yellow fever-endemic areas of the world for cities in non-endemic areas that appear suitable for viral transmission without having to provide proof of vaccination. Rapid global changes in human mobility and urbanization make it vital for countries to re-examine their vaccination policies and practices to prevent urban yellow fever epidemics.
Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus.

PubMed

Ou, Tong; Zhu, Ruo-Lin; Chen, Zhong-Yuan; Zhang, Qi-Ya

2013-11-06

We provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neurological signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 × 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease.
Invading species in the Eel River, California: Successes, failures, and relationships with resident species

USGS Publications Warehouse

Brown, L.R.; Moyle, P.B.

1997-01-01

We examined invasions of non-native fishes into the Eel River, California. At least 16 species of fish have been introduced into the drainage which originally supported 12-14 fish species. Our study was prompted by the unauthorized introduction in 1979 of Sacramento squawfish, Ptychocheilus grandis, a large predatory cyprinid. From 1986 to 1990, we conducted growth and diet studies of squaw fish, conducted intensive surveys of the distribution and habitat associations of both native and introduced species, and examined the nature of species-habitat and interspecies relationships. We found no evidence for increased growth or expanded feeding habits, compared to native populations, of Sacramento squawfish as they invaded the Eel River drainage. Ten of the introduced species were well established, with four species limited to a reservoir and six species established in streams. The success or failure of introductions of stream species appeared to be a function of the ability of a species to survive the fluctuating, highly seasonal, flow regime. The present mixture of native and exotic species has not formed stable fish assemblages but it seems likely that four habitat-associated assemblages will develop. The overall effect of the successful species introductions has been to assemble a group of species, with some exceptions, that are native to and occur together in many California streams. The assemblages now forming are similar to those found in other California streams. The assemblage characterized by squawfish and suckers is likely to be resistant to invasion, in the absence of human caused habitat modifications.
Using NASA's Giovanni System to Simulate Time-Series Stations in the Outflow Region of California's Eel River

NASA Technical Reports Server (NTRS)

Acker, James G.; Shen, Suhung; Leptoukh, Gregory G.; Lee, Zhongping

2012-01-01

Oceanographic time-series stations provide vital data for the monitoring of oceanic processes, particularly those associated with trends over time and interannual variability. There are likely numerous locations where the establishment of a time-series station would be desirable, but for reasons of funding or logistics, such establishment may not be feasible. An alternative to an operational time-series station is monitoring of sites via remote sensing. In this study, the NASA Giovanni data system is employed to simulate the establishment of two time-series stations near the outflow region of California s Eel River, which carries a high sediment load. Previous time-series analysis of this location (Acker et al. 2009) indicated that remotely-sensed chl a exhibits a statistically significant increasing trend during summer (low flow) months, but no apparent trend during winter (high flow) months. Examination of several newly-available ocean data parameters in Giovanni, including 8-day resolution data, demonstrates the differences in ocean parameter trends at the two locations compared to regionally-averaged time-series. The hypothesis that the increased summer chl a values are related to increasing SST is evaluated, and the signature of the Eel River plume is defined with ocean optical parameters.
21 CFR 137.290 - Self-rising yellow corn meal.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Self-rising yellow corn meal. 137.290 Section 137... Cereal Flours and Related Products § 137.290 Self-rising yellow corn meal. Self-rising yellow corn meal conforms to the definition and standard of identity prescribed by § 137.270 for self-rising white corn meal...
21 CFR 137.290 - Self-rising yellow corn meal.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Self-rising yellow corn meal. 137.290 Section 137... Cereal Flours and Related Products § 137.290 Self-rising yellow corn meal. Self-rising yellow corn meal conforms to the definition and standard of identity prescribed by § 137.270 for self-rising white corn meal...
Competition between nonindigenous ruffe and native yellow perch in laboratory studies

USGS Publications Warehouse

Savino, Jacqueline F.; Kolar, Cynthia S.

1996-01-01

The ruffe Gymnocephalus cernuus is a European percid that was accidently introduced in Duluth Harbor, Lake Superior. This nonindigenous species is closely related to yellow perch Perca flavescens, and because the two species have similar diets and habitat requirements, they are potential competitors. Laboratory studies in aquaria and pools were conducted to determine whether ruffe can compete with yellow perch for food. Ruffe had capture rates similar to those of yellow perch when food was unlimited. Ruffe spent more time than yellow perch over a feeding container before leaving it and searching again, and they also required less time to ingest (or handle) prey. However, the presence of yellow perch shortened the time ruffe spent over foraging areas when food was more limited. In addition, yellow perch were more active than ruffe, as indicated by their more frequent visits to a feeding container. Hence, the outcome of exploitative competition was not conclusive; ruffe appear to have the advantage in some behaviors, yellow perch in others. Ruffe were much more aggressive than yellow perch, and interference competition may be important in the interactions between these species. Our results indicate that ruffe might compete with native yellow perch.
Influence of Background Genome on Enzymatic Characteristics of Yellow (Ay/-, Avy/-) Mice

PubMed Central

Wolff, George L.; Pitot, Henry C.

1973-01-01

Identification of the fundamental polypeptide difference between yellow (Ay/-, Avy/-) and non-yellow mice is important for biomedical research because of the influence of the yellow genotype on normal and neoplastic growth and obesity. The complexity of the "yellow mouse syndrome" makes attainment of this objective dependent on the separation of those pleiotropic enzyme differences which are secondary, and depend on the background genome, from those which are primary, and depend primarily on the agouti locus genotype.—Four of nine hepatic enzyme activities assayed simultaneously differed between eight-week-old yellow (Ay/-, Avy/-) and non-yellow (A/-, a/a) male inbred and F1 hybrid mice. Among these four, only cytoplasmic malic enzyme activity was elevated in all yellow mice, as compared with the non-yellow sibs, regardless of background genome. Glucokinase, serine dehydratase, and tyrosine α-ketoglutarate transaminase activities were also changed in yellow mice, but these alterations depended on the background genome.—The ratio of malic enzyme activity to citrate-cleavage enzyme activity, possibly related to the altered fat metabolism of yellow mice, was influenced by background genome as well as by the yellow genotype.——Significant deviations of enzyme activities from mid-parent values among F1 hybrids were associated with particular background genomes; the number of such deviations was larger among yellow mice than among non-yellows and this difference was greater among C3H F1 hybrids than among C57BL/6 F1 hybrids. PMID:4405752
International travel between global urban centres vulnerable to yellow fever transmission

PubMed Central

Brent, Shannon E; Watts, Alexander; Cetron, Martin; German, Matthew; Kraemer, Moritz UG; Bogoch, Isaac I; Brady, Oliver J; Hay, Simon I; Creatore, Maria I

2018-01-01

Abstract Objective To examine the potential for international travel to spread yellow fever virus to cities around the world. Methods We obtained data on the international flight itineraries of travellers who departed yellow fever-endemic areas of the world in 2016 for cities either where yellow fever was endemic or which were suitable for viral transmission. Using a global ecological model of dengue virus transmission, we predicted the suitability of cities in non-endemic areas for yellow fever transmission. We obtained information on national entry requirements for yellow fever vaccination at travellers’ destination cities. Findings In 2016, 45.2 million international air travellers departed from yellow fever-endemic areas of the world. Of 11.7 million travellers with destinations in 472 cities where yellow fever was not endemic but which were suitable for virus transmission, 7.7 million (65.7%) were not required to provide proof of vaccination upon arrival. Brazil, China, India, Mexico, Peru and the United States of America had the highest volumes of travellers arriving from yellow fever-endemic areas and the largest populations living in cities suitable for yellow fever transmission. Conclusion Each year millions of travellers depart from yellow fever-endemic areas of the world for cities in non-endemic areas that appear suitable for viral transmission without having to provide proof of vaccination. Rapid global changes in human mobility and urbanization make it vital for countries to re-examine their vaccination policies and practices to prevent urban yellow fever epidemics. PMID:29875519
Photoluminescence and cathodoluminescence of Mn doped zinc silicate nanophosphors for green and yellow field emissions displays

NASA Astrophysics Data System (ADS)

Omri, K.; Alyamani, A.; Mir, L. El

2018-02-01

Mn2+-doped Zn2SiO4 (ZSM2+) was synthesized by a facile sol-gel technique. The obtained samples were characterized by X-ray diffraction (XRD), Raman spectroscopy, photoluminescence (PL) and cathodoluminescence (CL) techniques. Under UV excitation, spectra showed that the α-ZSM2+ phosphor exhibited a strong green emission around 525 nm and reached the highest luminescence intensity with the Mn doping concentration of 5 at.%. However, for the β-ZSM2+ phase, an interesting yellow emission band centered at 575 nm of Mn2+ at the Zn2+ tetrahedral sites was observed. In addition, an unusual red shift with increasing Mn2+ content was also found and attributed to an exchange interaction between Mn2+. Both PL and CL spectra exhibit an intense green and yellow emission centered at 525 and 573 nm, respectively, due to the 4T1 (4G)-6A1 (6S) transition of Mn2+. Furthermore, these results indicated that the Mn2+-doped zinc silicate phosphors may have potential applications in green and yellow emissions displays like field emission displays (FEDs).
The developing stages of the Martian yellow storm of 1971

NASA Technical Reports Server (NTRS)

Capen, C. F.; Martin, L. J.

1971-01-01

A history of the yellow storm on Mars which occurred in 1971 is presented. It is compared to the Great 1956 Yellow Cloud, and possible yellow storms are predicted for 1973. Photographs of the stages of evolution and the path of the storm are included.
Ceres' Yellow Spots - Observations with Dawn Framing Camera

NASA Astrophysics Data System (ADS)

Schäfer, Michael; Schäfer, Tanja; Cloutis, Edward A.; Izawa, Matthew R. M.; Platz, Thomas; Castillo-Rogez, Julie C.; Hoffmann, Martin; Thangjam, Guneshwar S.; Kneissl, Thomas; Nathues, Andreas; Mengel, Kurt; Williams, David A.; Kallisch, Jan; Ripken, Joachim; Russell, Christopher T.

2016-04-01

The Framing Camera (FC) onboard the Dawn spacecraft acquired several spectral data sets of (1) Ceres with increasing spatial resolution (up to 135 m/pixel with nearly global coverage). The FC is equipped with seven color filters (0.4-1.0 μm) plus one panchromatic ('clear') filter [1]. We produced spectral mosaics using photometrically corrected FC color filter images as described in [2]. Even early FC color mosaics obtained during Dawn's approach unexpectedly exhibited quite a diversity of surface materials on Ceres. Besides the ordinary cerean surface material, potentially composed of ammoniated phyllosilicates [3] or some other alteration product of carbonaceous chondrites [4], a large number of bright spots were found on Ceres [5]. These spots are substantially brighter than the average surface (exceeding its triple standard deviation), with the spots within Occator crater being the brightest and most prominent examples (reflectance more than 10 times the average of Ceres). We observed bright spots which are different by their obvious yellow color. This yellow color appears both in a 'true color' RGB display (R=0.65, G=0.55, B=0.44 μm) as well as in a false color display (R=0.97, G=0.75, B=0.44 μm) using a linear 2% stretch. Their spectra show a steep red slope between 0.44 and 0.55 μm (UV drop-off). On the contrary to these yellow spots, the vast majority of bright spots appears white in the aforementioned color displays and exhibit blue sloped spectra, except for a shallow UV drop-off. Thus, yellow spots are easily distinguishable from white spots and the remaining cerean surface by their high values in the ratio 0.55/0.44 μm. We found 8 occurrences of yellow spots on Ceres. Most of them (>70 individual spots) occur both inside and outside crater Dantu, where white spots are also found in the immediate vicinity. Besides Dantu, further occurrences with only a few yellow spots were found at craters Ikapati and Gaue. Less definite occurrences are found at 97
Beyond the angle of repose: A review and synthesis of landslide processes in response to rapid uplift, Eel River, Northern California

NASA Astrophysics Data System (ADS)

Roering, Joshua J.; Mackey, Benjamin H.; Handwerger, Alexander L.; Booth, Adam M.; Schmidt, David A.; Bennett, Georgina L.; Cerovski-Darriau, Corina

2015-05-01

In mountainous settings, increases in rock uplift are often followed by a commensurate uptick in denudation as rivers incise and steepen hillslopes, making them increasingly prone to landsliding as slope angles approach a limiting value. For decades, the threshold slope model has been invoked to account for landslide-driven increases in sediment flux that limit topographic relief, but the manner by which slope failures organize themselves spatially and temporally in order for erosion to keep pace with rock uplift has not been well documented. Here, we review past work and present new findings from remote sensing, cosmogenic radionuclides, suspended sediment records, and airborne lidar data, to decipher patterns of landslide activity and geomorphic processes related to rapid uplift along the northward-migrating Mendocino Triple Junction in Northern California. From historical air photos and airborne lidar, we estimated the velocity and sediment flux associated with active, slow-moving landslides (or earthflows) in the mélange- and argillite-dominated Eel River watershed using the downslope displacement of surface markers such as trees and shrubs. Although active landslides that directly convey sediment into the channel network account for only 7% of the landscape surface, their sediment flux amounts to more than 50% of the suspended load recorded at downstream sediment gaging stations. These active slides tend to exhibit seasonal variations in velocity as satellite-based interferometry has demonstrated that rapid acceleration commences within 1 to 2 months of the onset of autumn rainfall events before slower deceleration ensues in the spring and summer months. Curiously, this seasonal velocity pattern does not appear to vary with landslide size, suggesting that complex hydrologic-mechanical feedbacks (rather than 1-D pore pressure diffusion) may govern slide dynamics. A new analysis of 14 yrs of discharge and sediment concentration data for the Eel River indicates
Phylodynamics of Yellow Fever Virus in the Americas: new insights into the origin of the 2017 Brazilian outbreak.

PubMed

Mir, Daiana; Delatorre, Edson; Bonaldo, Myrna; Lourenço-de-Oliveira, Ricardo; Vicente, Ana Carolina; Bello, Gonzalo

2017-08-07

Yellow fever virus (YFV) strains circulating in the Americas belong to two distinct genotypes (I and II) that have diversified into several concurrent enzootic lineages. Since 1999, YFV genotype I has spread outside endemic regions and its recent (2017) reemergence in non-endemic Southeastern Brazilian states fuels one of the largest epizootic of jungle Yellow Fever registered in the country. To better understand this phenomenon, we reconstructed the phylodynamics of YFV American genotypes using sequences from nine countries sampled along 60 years, including strains from Brazilian 2017 outbreak. Our analyses reveals that YFV genotypes I and II follow roughly similar evolutionary and demographic dynamics until the early 1990s, when a dramatic change in the diversification process of the genotype I occurred associated with the emergence and dissemination of a new lineage (here called modern). Trinidad and Tobago was the most likely source of the YFV modern-lineage that spread to Brazil and Venezuela around the late 1980s, where it replaced all lineages previously circulating. The modern-lineage caused all major YFV outbreaks detected in non-endemic South American regions since 2000, including the 2017 Brazilian outbreak, and its dissemination was coupled to the accumulation of several amino acid substitutions particularly within non-structural viral proteins.
Availability of yellow pine sawtimber in Alabama

Treesearch

William H. McWilliams

1991-01-01

Alabama's timberland supports 76.2 billion board feet of sawtimber (International 1/4-inch Rule), of which 55 percent is contributed by yellow pine species. Currently, yellow pine sawtimber volume totals 41.8 billion board feet. The recent inventory conducted by the USDA-Forest Service, Southern Forest Experiment Station, Forest Inventory and Analysis Unit (SO-...
Yellowing reaction in encapsulant of photovoltaic modules

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shigekuni, T.; Kumano, M.

1997-12-31

To clarify the mechanism of the yellowing reaction in encapsulant used for photovoltaic (PV) modules, a low molecular weight substance in EVA (Ethylene vinyl acetate) under accelerated weathering test (Dew cycle test, 1000 hours) with yellow change and virgin EVA were extracted with methanol. Extracts were chemically analyzed by GCIR (Gas Chromatography Infrared-Ray spectroscopic analysis), GC-AED (Gas Chromatography Atomic Emission Detector), and FDMS (Field Desorption Mass Spectroscopy). The conditions of this accelerated test were based on JIS-K9117. The analysis results showed that 2,6-di-t-butyl-4-methyl phenol of antioxidant and 2-hydroxy-4-octoxy-benzophenone of UV absorbent were consumed after the weathering test and that 3,5-di-t-butyl-4-hydroxy-benzaldehydemore » having yellow color was newly produced. A mechanism of the yellowing reaction in encapsulant was presented here that 2,6-di-t-N-O radical from Bis-2,2,6,6-tetramethyl-4-piperidinyl sebacate to produce 3,5 di-t-butyl-4-hydroxy benzaldehyde.« less
A novel emaravirus is associated with redbud yellow ringspot disease

USDA-ARS?s Scientific Manuscript database

Yellow ringspot is the only virus-like disease reported in redbud (Cercis spp.) with symptoms including vein clearing, chlorotic ringspots and oak-leaf pattern. A putative new emaravirus was present in 48 of 48l trees displaying typical yellow ringspot symptoms and the name redbud yellow ringspot as...
Shelf-life extension of preservative-free hydrated feed using gamma pasteurization and its effect on growth performance of eel

NASA Astrophysics Data System (ADS)

Kim, Dongho; Song, Hyunpa; Lim, Sangyong; Jo, Minho; Song, Duseop; Jo, Cheorun

2012-08-01

Hydrated feed (HF) promotes the growth performance and shortens the feeding time of fish by increasing the efficiency of digestion. However, the shelf-life of HF is a concern due to its relatively higher water content. In this study, radiation pasteurization was applied to improve the shelf-life and microbiological quality of HF for fish farming. Preservative-free HF containing 25% moisture was gamma-irradiated and its microbiological and nutritional properties evaluated in addition to a practical feeding trial carried out using eel. The viable counts of bacteria and fungi in HF were 106 and 104 CFU/g, respectively. All coliform bacteria and yeast in HF were eliminated by irradiation at a dose of 5 kGy, and total aerobic bacteria were eliminated at 10 kGy. The shelf-life of the preservative-free and irradiated (10 kGy) HF was estimated as 6 months under ambient conditions. The nutritional composition of HF was stable up to 10 kGy of irradiation. Based on a feeding trial, it was proven that eel fed HF had about 20% higher growth rate than that fed dried feed.
Optimization of Replacing Pork Meat with Yellow Worm (Tenebrio molitor L.) for Frankfurters

PubMed Central

Paik, Hyun-Dong

2017-01-01

The effects of replacing pork meat with yellow mealworms on the physicochemical properties and sensory characteristics of frankfurters were investigated in this study. The control (50% pork ham), T1 (45% pork ham + 5% yellow mealworm), T2 (40% pork ham + 10% yellow mealworm), T3 (35% pork ham + 15% yellow mealworm), T4 (30% pork ham + 20% yellow mealworm), T5 (25% pork ham + 25% yellow mealworm), and T6 (20% pork ham + 30% yellow mealworm) were prepared, replacing lean pork meat with yellow mealworm. The moisture content, lightness, sarcoplasmic protein solubility, hardness, gumminess, chewiness, and apparent viscosity of frankfurters with yellow mealworm were lower than those of the control (p<0.05), whereas the content of protein and ash, pH, and yellowness of frankfurters with yellow mealworm were higher than those of the control (p<0.05). The fat content of frankfurters in T1 (p<0.05) was the highest, and the fat content of treatments decreased with increasing yellow mealworm concentrations (p<0.05). Frankfurters with increasing yellow mealworm concentrations had lower color, flavor, off-flavor, and juiciness scores. The overall acceptability was not significantly different in the control, T1, and T2 (p>0.05). Thus, the results of this study showed that replacing lean pork meat with up to 10% yellow mealworm successfully maintained the quality of frankfurters at a level similar to that of the regular control frankfurters. PMID:29147084

[The use of white and yellow turpentine baths with diabetic patients].

PubMed

Davydova, O B; Turova, E A; Golovach, A V

1998-01-01

In patients with insulin-dependent diabetes mellitus while and yellow turpentine baths produced a positive effect on carbohydrate metabolism. White baths were more effective in respect to lipid metabolism, blood viscosity, produced a good effect on plasmic hemocoagulation factors. Both while and yellow turpentine baths were beneficial for capillary blood flow: initially high distal blood flow in patients with prevailing distal polyneuropathy decreased while in patients with macroangiopathy initially subnormal blood flow increased. Both white and yellow turpentine baths promoted better pulse blood filling of the lower limbs and weaker peripheral resistance of large vessels. In patients with non-insulin-dependent diabetes mellitus white and yellow turpentine baths contributed to normalization of carbohydrate metabolism. Yellow baths were more effective in lowering lipids. White baths induced inhibition of platelet aggregation but had no effect on coagulation, yellow baths promoted a reduction of fibrinogen but had no effect on platelet aggregation. Yellow baths produced more pronounced effect than white ones on blood viscosity and microcirculation. Both yellow and white baths stimulated pulse blood filling, corrected peripheral resistance of large and small vessels of the lower limbs.
Yellow Fever outbreaks in unvaccinated populations, Brazil, 2008-2009.

PubMed

Romano, Alessandro Pecego Martins; Costa, Zouraide Guerra Antunes; Ramos, Daniel Garkauskas; Andrade, Maria Auxiliadora; Jayme, Valéria de Sá; Almeida, Marco Antônio Barreto de; Vettorello, Kátia Campomar; Mascheretti, Melissa; Flannery, Brendan

2014-03-01

Due to the risk of severe vaccine-associated adverse events, yellow fever vaccination in Brazil is only recommended in areas considered at risk for disease. From September 2008 through June 2009, two outbreaks of yellow fever in previously unvaccinated populations resulted in 21 confirmed cases with 9 deaths (case-fatality, 43%) in the southern state of Rio Grande do Sul and 28 cases with 11 deaths (39%) in Sao Paulo state. Epizootic deaths of non-human primates were reported before and during the outbreak. Over 5.5 million doses of yellow fever vaccine were administered in the two most affected states. Vaccine-associated adverse events were associated with six deaths due to acute viscerotropic disease (0.8 deaths per million doses administered) and 45 cases of acute neurotropic disease (5.6 per million doses administered). Yellow fever vaccine recommendations were revised to include areas in Brazil previously not considered at risk for yellow fever.
Dengue-yellow fever sera cross-reactivity; challenges for diagnosis.

PubMed

Houghton-Triviño, Natalia; Montaña, Diana; Castellanos, Jaime

2008-01-01

The Flavivirus genera share epitopes inducing cross-reactive antibodies leading to great difficulty in differentially diagnosing flaviviral infections. This work was aimed at evaluating the complexity of dengue and yellow fever serological differential diagnosis. Dengue antibody capture ELISA and a yellow fever neutralisation test were carried out on 13 serum samples obtained from yellow fever patients, 20 acute serum samples from dengue patients and 19 voluntary serum samples pre- and post-vaccination with YF vaccine. Dengue ELISA revealed IgM reactivity in 46,2 % of yellow fever patients and 42 % of vaccinees. Sixteen out of 20 dengue patients (80 %) had high YF virus neutralisation titres. Such very high cross-reactivity data challenged differential laboratory diagnosis of dengue and yellow fever in areas where both flaviviruses co-circulate. New laboratory strategies are thus needed for improving the tests and providing a specific laboratory diagnosis. Cross-reactivity between Flaviviruses represents a great difficulty for epidemiological surveillance and preventing dengue, both of which demand urgent attention.
Decomposition of L a 2 – x S r x Cu O 4 into several L a 2 O 3 phases at elevated temperatures in ultrahigh vacuum inside a transmission electron microscope

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jeong, Jong Seok; Wu, Wangzhou; Topsakal, Mehmet

Here, we report the decomposition of La 2–xSr xCuO 4 into La 2O 3 and Cu nanoparticles in ultrahigh vacuum, observed by in situ heating experiments in a transmission electron microscope. The analysis of electron diffraction data reveals that the phase decomposition process starts at about 150°C and is considerably expedited in the temperature range of 350°C–450°C. Two major resultant solid phases are identified as metallic Cu and La 2O 3 by electron diffraction, simulation, and electron energy-loss spectroscopy (EELS) analyses. With the aid of calculations, La 2O 3 phases are further identified to be derivatives of a fluorite structure—fluorite,more » pyrochlore, and (distorted) bixbyite—characterized by different oxygen-vacancy order. Additionally, the bulk plasmon energy and the fine structures of the O K and LaM 4,5 EELS edges are reported for these structures, along with simulated O K x-ray absorption near-edge structure. The resultant Cu nanoparticles and La 2O 3 phases remain unchanged after cooling to room temperature.« less
Decomposition of L a 2 – x S r x Cu O 4 into several L a 2 O 3 phases at elevated temperatures in ultrahigh vacuum inside a transmission electron microscope

DOE PAGES

Jeong, Jong Seok; Wu, Wangzhou; Topsakal, Mehmet; ...

2018-05-15

Here, we report the decomposition of La 2–xSr xCuO 4 into La 2O 3 and Cu nanoparticles in ultrahigh vacuum, observed by in situ heating experiments in a transmission electron microscope. The analysis of electron diffraction data reveals that the phase decomposition process starts at about 150°C and is considerably expedited in the temperature range of 350°C–450°C. Two major resultant solid phases are identified as metallic Cu and La 2O 3 by electron diffraction, simulation, and electron energy-loss spectroscopy (EELS) analyses. With the aid of calculations, La 2O 3 phases are further identified to be derivatives of a fluorite structure—fluorite,more » pyrochlore, and (distorted) bixbyite—characterized by different oxygen-vacancy order. Additionally, the bulk plasmon energy and the fine structures of the O K and LaM 4,5 EELS edges are reported for these structures, along with simulated O K x-ray absorption near-edge structure. The resultant Cu nanoparticles and La 2O 3 phases remain unchanged after cooling to room temperature.« less
42 CFR 71.3 - Designation of yellow fever vaccination centers; Validation stamps.

Code of Federal Regulations, 2010 CFR

2010-10-01

... safe, potent, and pure yellow fever vaccine. Medical facilities of Federal agencies are authorized to obtain yellow fever vaccine without being designated as a yellow fever vaccination center by the Director..., storage, and administration of yellow fever vaccine. If a designated center fails to comply with such...
Behaviour of wintering Tundra Swans Cygnus columbianus columbianus at the Eel River delta and Humboldt Bay, California, USA

USGS Publications Warehouse

Black, Jeffrey M.; Gress, Carol; Byers, Jacob W.; Jennings, Emily; Ely, Craig R.

2010-01-01

Tundra Swan Cygnus columbianus columbinanus phenology and behaviour at the Eel River delta and southern Humboldt Bay in northern California, USA, is described. Counts made each January from 1963 onwards peaked at 1,502 swans in 1988. Monthly counts recorded during the 2006/07 and 2008/09 winters peaked in February, at 1,033 and 772 swans respectively. Swans roosted on ephemeral ponds at the Humboldt Bay National Wildlife Refuge, on ephemeral ponds within grassland pastures in the vicinity of the Refuge, and perhaps also used the Eel River as a roost. Flights between Refuge roosts and the pastures and ponds occurred in the two hours after sunrise and before dark. In winters 2008/09 and 2009/10, the percentage of cygnets in the flocks was 10.6% and 21.4% respectively, and increased to =31% cygnets each year after most swans had departed from the area in March. Average brood size in 2009/10 was 2.1 cygnets. Daily activities consisted of foraging (44.9% of activities recorded), comfort behaviour (22.1%), locomotion (16.2%) and vigilance (15.5%). Eight neck-collared swans identified in the wintering flock were marked at four locations in different parts of Alaska, up to 1,300 km apart.
A Phase One Archaeological Survey of the Cottonwood, Redwood and Yellow Medicine Drainages in Southwestern Minnesota

DTIC Science & Technology

1989-03-01

and June 15, a three person field team conducted the archaeological survey in the Cottonwood, Redwood, and Yellow Medicine subbasins. The project...discovering sites that are exposed on the ground surface. A three- person survey team can examine between 100 and 150 acres per day, depending on field...results are dependent on the interval used between individual shovel tests. When 15 meter intervals between tests are used, we estimate that one person
Yellow fever in China is still an imported disease.

PubMed

Chen, Jun; Lu, Hongzhou

2016-05-23

Yellow fever is a vector-borne disease endemic to tropical regions of Africa and South America. A recent outbreak in Angola caused hundreds of deaths. Six cases of yellow fever imported from Angola were reported recently in China. This raised the question of whether it will spread in China and how it can be prevented. This article discusses the possibility of yellow fever transmission in China and the strategies to counter it.
Development of a Real-Time Reverse Transcription-PCR Assay for Global Differentiation of Yellow Fever Virus Vaccine-Related Adverse Events from Natural Infections.

PubMed

Hughes, Holly R; Russell, Brandy J; Mossel, Eric C; Kayiwa, John; Lutwama, Julius; Lambert, Amy J

2018-06-01

Yellow fever (YF) is a reemerging public health threat, with frequent outbreaks prompting large vaccination campaigns in regions of endemicity in Africa and South America. Specific detection of vaccine-related adverse events is resource-intensive, time-consuming, and difficult to achieve during an outbreak. To address this, we have developed a highly transferable rapid yellow fever virus (YFV) vaccine-specific real-time reverse transcription-PCR (RT-PCR) assay that distinguishes vaccine from wild-type lineages. The assay utilizes a specific hydrolysis probe that includes locked nucleic acids to enhance specific discrimination of the YFV17D vaccine strain genome. Promisingly, sensitivity and specificity analyses reveal this assay to be highly specific to vaccine strain(s) when tested on clinical samples and YFV cell culture isolates of global origin. Taken together, our data suggest the utility of this assay for use in laboratories of varied capacity for the identification and differentiation of vaccine-related adverse events from wild-type infections of both African and South American origin. Copyright © 2018 American Society for Microbiology.
Epigenetic modifications during sex change repress gonadotropin stimulation of cyp19a1a in a teleost ricefield eel (Monopterus albus).

PubMed

Zhang, Yang; Zhang, Shen; Liu, Zhixin; Zhang, Lihong; Zhang, Weimin

2013-08-01

In vertebrates, cytochrome P450 aromatase, encoded by cyp19a1, converts androgens to estrogens and plays important roles in gonadal differentiation and development. The present study examines whether epigenetic mechanisms are involved in cyp19a1a expression and subsequent gonadal development in the hermaphroditic ricefield eel. The expression of the ricefield eel cyp19a1a was stimulated by gonadotropin via the cAMP pathway in the ovary but not the ovotestis or testis. The CpG within the cAMP response element (CRE) of the cyp19a1a promoter was hypermethylated in the ovotestis and testis compared with the ovary. The methylation levels of CpG sites around CRE in the distal region (region II) and around steroidogenic factor 1/adrenal 4 binding protein sites and TATA box in the proximal region (region I) were inversely correlated with cyp19a1a expression during the natural sex change from female to male. In vitro DNA methylation decreased the basal and forskolin-induced activities of cyp19a1a promoter. Chromatin immunoprecipitation assays indicated that histone 3 (Lys9) in both regions I and II of the cyp19a1a promoter were deacetylated and trimethylated in the testis, and in contrast to the ovary, phosphorylated CRE-binding protein failed to bind to these regions. Lastly, the DNA methylation inhibitor 5-aza-2'-deoxycytidine reversed the natural sex change of ricefield eels. These results suggested that epigenetic mechanisms involving DNA methylation and histone deacetylation and methylation may abrogate the stimulation of cyp19a1a by gonadotropins in a male-specific fashion. This may be a mechanism widely used to drive natural sex change in teleosts as well as gonadal differentiation in other vertebrates.
Serologic assessment of yellow fever immunity in the rural population of a yellow fever-endemic area in Central Brazil.

PubMed

Machado, Vanessa Wolff; Vasconcelos, Pedro Fernando da Costa; Silva, Eliana Vieira Pinto; Santos, João Barberino

2013-01-01

The yellow fever epidemic that occurred in 1972/73 in Central Brazil surprised the majority of the population unprotected. A clinical-epidemiological survey conducted at that time in the rural area of 19 municipalities found that the highest (13.8%) number of disease cases were present in the municipality of Luziânia, State of Goiás. Thirty-eight years later, a new seroepidemiological survey was conducted with the aim of assessing the degree of immune protection of the rural population of Luziânia, following the continuous attempts of public health services to obtain vaccination coverage in the region. A total of 383 volunteers, aged between 5 and 89 years and with predominant rural labor activities (75.5%), were interviewed. The presence of antibodies against the yellow fever was also investigated in these individuals, by using plaque reduction neutralization test, and correlated to information regarding residency, occupation, epidemiological data and immunity against the yellow fever virus. We found a high (97.6%) frequency of protective titers (>1:10) of neutralizing antibodies against the yellow fever virus; the frequency of titers of 1:640 or higher was 23.2%, indicating wide immune protection against the disease in the study population. The presence of protective immunity was correlated to increasing age. This study reinforces the importance of surveys to address the immune state of a population at risk for yellow fever infection and to the surveillance of actions to control the disease in endemic areas.
Experimental investigation of efficient locomotion of underwater snake robots for lateral undulation and eel-like motion patterns.

PubMed

Kelasidi, Eleni; Liljebäck, Pål; Pettersen, Kristin Y; Gravdahl, Jan T

2015-01-01

Underwater snake robots offer many interesting capabilities for underwater operations. The long and slender structure of such robots provide superior capabilities for access through narrow openings and within confined areas. This is interesting for inspection and monitoring operations, for instance within the subsea oil and gas industry and within marine archeology. In addition, underwater snake robots can provide both inspection and intervention capabilities and are thus interesting candidates for the next generation inspection and intervention AUVs. Furthermore, bioinspired locomotion through oscillatory gaits, like lateral undulation and eel-like motion, is interesting from an energy efficiency point of view. Increasing the motion efficiency in terms of the achieved forward speed by improving the method of propulsion is a key issue for underwater robots. Moreover, energy efficiency is one of the main challenges for long-term autonomy of these systems. In this study, we will consider both these two aspects of efficiency. This paper considers the energy efficiency of swimming snake robots by presenting and experimentally investigating fundamental properties of the velocity and the power consumption of an underwater snake robot for both lateral undulation and eel-like motion patterns. In particular, we investigate the relationship between the parameters of the gait patterns, the forward velocity and the energy consumption for different motion patterns. The simulation and experimental results are seen to support the theoretical findings.
New developments in flavivirus vaccines with special attention to yellow fever.

PubMed

Pugachev, Konstantin V; Guirakhoo, Farshad; Monath, Thomas P

2005-10-01

Here we review recent epidemiological trends in flavivirus diseases, findings related to existing vaccines, and new directions in flavivirus vaccine research. We emphasize the need for stepped-up efforts to stop further spread and intensification of these infections worldwide. Although the incidence and geographic distribution of flavivirus diseases have increased in recent years, human vaccines are available only for yellow fever, Japanese encephalitis, tick-borne encephalitis and Kyasanur forest disease. Factors contributing to resurgence include insufficient supplies of available vaccines, incomplete vaccination coverage and relaxation in vector control. Research has been underway for 60 years to develop effective vaccines against dengue, and recent progress is encouraging. The development of vaccines against West Nile, virus recently introduced to North America, has been initiated. In addition, there is considerable interest in improving existing vaccines with respect to increasing safety (e.g. eliminating the newly recognized syndrome of yellow fever vaccine-associated viscerotropic adverse disease), and to reducing the cost and number of doses required for effective immunization. Traditional approaches to flavivirus vaccines are still employed, while recent advancements in biotechnology produced new approaches to vaccine design, such as recombinant live virus, subunit and DNA vaccines. Live chimeric vaccines against dengue, Japanese encephalitis and West Nile based on yellow fever 17D virus (ChimeriVax) are in phase I/II trials, with encouraging results. Other chimeric dengue, tick-borne encephalitis and West Nile virus candidates were developed based on attenuated dengue backbones. To further reduce the impact of flavivirus diseases, vaccination policies and vector control programs in affected countries require revision.
Yellow fever vaccine: an effective vaccine for travelers.

PubMed

Verma, Ramesh; Khanna, Pardeep; Chawla, Suraj

2014-01-01

Yellow fever (YF) is an acute viral communicable disease transmitted by an arbovirus of the Flavivirus genus. It is primarily a zoonotic disease, especially the monkeys. Worldwide, an estimated 200,000 cases of yellow fever occurred each year, and the case-fatality rate is ~15%. Forty-five endemic countries in Africa and Latin America, with a population of close to 1 billion, are at risk. Up to 50% of severely affected persons from YF die without treatment. During 2009, 55 cases and 18 deaths were reported from Brazil, Colombia, and Peru. Brazil reported the maximum number of cases and death, i.e., 42 cases with 11 deaths. From January 2010 to March 2011, outbreaks of YF were reported to the WHO by Cameroon, Democratic Republic of Congo, Cote d'Ivoire, Guinea, Sierra Leone, Senegal, and Uganda. Cases were also reported in three northern districts of Abim, Agago, and Kitugun near the border with South Sudan. YF usually causes fever, muscle pain with prominent backache, headache, shivers, loss of appetite, and nausea or vomiting. Most patients improve, and their symptoms disappear after 3 to 4 d. Half of the patients who enter the toxic phase die within 10-14 d, while the rest recover without significant organ damage. Vaccination has been the single most important measure for preventing YF. The 17D-204 YF vaccine is a freeze-dried, live attenuated, highly effective vaccine. It is available in single-dose or multi-dose vials and should be stored at 2-8 °C. It is reconstituted with normal saline and should be used within 1 h of reconstitution. The 0.5 mL dose is delivered subcutaneously. Revaccination is recommended every 10 y for people at continued risk of exposure to yellow fever virus (YFV). This vaccine is available worldwide. Travelers, especially to Africa or Latin America from Asia, must have a certificate documenting YF vaccination, which is required by certain countries for entry under the International Health Regulations (IHR) of the WHO.
Maturation and Collection of Yellow-Poplar Seeds in the Midsouth

Treesearch

F. T. Bonner

1976-01-01

Yellow-poplar fruits are best collected in late October when their color changes from green to yellow-green or yellow. There were no other obvious physical or chemical changes indicating maturity. The seeds are physiologically mature as early as September 1, although high fruit moisture contents make special handling necessary if fruits are collected at this time....
Variability of yellow tulp (Moraea pallida Bak.) toxicity.

PubMed

Snyman, L D; Schultz, R A; van den Berg, H

2011-06-01

Yellow tulp (Moraea pallida Bak.), collected predominantly during the flowering stage from a number of sites in South Africa, showed large variation in digoxin equivalent values, indicating variability in yellow tulp toxicity. Very low values were recorded for tulp collected from certain sites in the Northern Cape.
Isolation and characterization of a Brazilian strain of yellow fever virus from an epizootic outbreak in 2009.

PubMed

Jorge, Taissa Ricciardi; Mosimann, Ana Luiza Pamplona; Noronha, Lucia de; Maron, Angela; Duarte Dos Santos, Claudia Nunes

2017-02-01

During a series of epizootics caused by Yellow fever virus in Brazil between 2007 and 2009, a monkey was found dead (May 2009) in a sylvatic area in the State of Paraná. Brain samples from this animal were used for immunohistochemical analysis and isolation of a wild-type strain of YFV. This viral strain was characterized, and sequence analyzes demonstrated that it is closely related with YFV strains of the recently identified subclade 1E of the South American genotype I. Further characterization included indirect-immunofluorescence of different infected cell lines and analysis of the kinetics of virus replication and infectivity inhibition by type I IFN. The generated data contributes to the knowledge of YFV evolution and phylogeny. Additionally, the reagents generated and characterized during this study, such as a panel of monoclonal antibodies, are useful tools for further studies on YFV. Lastly, this case stresses the importance of yellow fever surveillance through sentinel monkeys. Copyright © 2016 Elsevier B.V. All rights reserved.
Highly pure yellow light emission of perovskite CsPb(BrxI1-x)3 quantum dots and their application for yellow light-emitting diodes

NASA Astrophysics Data System (ADS)

He, Yuandan; Gong, Jinhui; Zhu, Yiyuan; Feng, Xingcan; Peng, Hong; Wang, Wei; He, Haiyang; Liu, Hu; Wang, Li

2018-06-01

High-quality all-inorganic perovskite CsPb(BrxI1-x)3 quantum dots (QDs) with quantum yield of 50% were systematically studied as yellow light convertor for light emitting diodes (LEDs). A novel heat insulation structure was designed for the QD-converted yellow LEDs. In this structure, a silicone layer was set on top of the GaN LED chip to prevent directly heating of the QDs by the LED chip. Then the CsPb(BrxI1-x)3 QDs were filled in the bowl-shaped silicone layer after ultrasonic dispersion treatment. Finally, an Al2O3 passivation layer was grown on the QDs layer by Atomic Layer Disposition at 40 °C. When x = 0.55, highly pure yellow LEDs with an emission peak at ∼570 nm and a full width at half maximum of 25 nm were achieved. The chromaticity coordinates of the QD-converted yellow LEDs (0.4920 ± 0.0017, 0.4988 ± 0.0053) showed almost no variation under driving current from 5 mA to 150 mA. During an operation period of 60 min, the emission wavelength of the yellow LEDs showed no distinct shift. Moreover, the luminous efficiency of the QD-converted yellow LEDs achieved 13.51 l m/W at 6 mA. These results demonstrated that CsPb(BrxI1-x)3 QDs and the heat insulation structure are promising candidate for high purity yellow LEDs.
[Trends in yellow fever mortality in Colombia, 1998-2009].

PubMed

Segura, Ángela María; Cardona, Doris; Garzón, María Osley

2013-09-01

Yellow fever is a neglected tropical disease, thus, knowing the trends in mortality from this disease in Colombia is an important source of information for decision making and identifying public health interventions. To analyze trends in yellow fever mortality in Colombia during the 1998-2009 period and the differences in the morbidity and mortality information sources for the country, which affect indicators such as the lethality one. This is a descriptive study of deaths by yellow fever according to the Departamento Administrativo Nacional de Estadística and the incidence of the disease according to the Instituto Nacional de Salud . We used secondary sources of information in the calculation of proportions of socio-demographic characteristics of the deceased and epidemiological measures of lethality, incidence and mortality from yellow fever by department of residence of the deceased. Yellow fever deaths occur primarily in men of working age residing in scattered rural areas, who were members of the regimen vinculado, and who were living in the eastern, southeastern, northern and central zones in the country. We observed inconsistencies in the reports that affect the comparative analysis. The inhabitants of the departments located in national territories and Norte de Santander have an increased risk of illness and death from yellow fever, but this information could be underestimated, according to the source of information used for its calculation.

Effects of salinity and sea salt type on egg activation, fertilization, buoyancy and early embryology of European eel, Anguilla anguilla.

PubMed

Sørensen, Sune Riis; Butts, Ian Anthony Ernest; Munk, Peter; Tomkiewicz, Jonna

2016-02-01

Improper activation and swelling of in vitro produced eggs of European eel, Anguilla anguilla, has been shown to negatively affect embryonic development and hatching. We investigated this phenomenon by examining the effects of salinity and sea salt type on egg dimensions, cell cleavage patterns and egg buoyancy. Egg diameter after activation, using natural seawater adjusted to different salinities, varied among female eels, but no consistent pattern emerged. Activation salinities between 30-40 practical salinity unit (psu) produced higher quality eggs and generally larger egg diameters. Chorion diameters reached maximal values of 1642 ± 8 μm at 35 psu. A positive relationship was found between egg neutral buoyancy and activation salinity. Nine salt types were investigated as activation and incubation media. Five of these types induced a substantial perivitelline space (PVS), leading to large egg sizes, while the remaining four salt types resulted in smaller eggs. All salt types except NaCl treatments led to high fertilization rates and had no effect on fertilization success as well as egg neutral buoyancies at 7 h post-fertilization. The study points to the importance of considering ionic composition of the media when rearing fish eggs and further studies are encouraged.
Environmental impact assessments of the Xiaolangdi Reservoir on the most hyperconcentrated laden river, Yellow River, China.

PubMed

Kong, Dongxian; Miao, Chiyuan; Wu, Jingwen; Borthwick, Alistair G L; Duan, Qingyun; Zhang, Xiaoming

2017-02-01

The Yellow River is the most hyperconcentrated sediment-laden river in the world. Throughout recorded history, the Lower Yellow River (LYR) experienced many catastrophic flood and drought events. To regulate the LYR, a reservoir was constructed at Xiaolangdi that became operational in the early 2000s. An annual water-sediment regulation scheme (WSRS) was then implemented, aimed at flood control, sediment reduction, regulated water supply, and power generation. This study examines the eco-environmental and socioenvironmental impacts of Xiaolangdi Reservoir. In retrospect, it is found that the reservoir construction phase incurred huge financial cost and required large-scale human resettlement. Subsequent reservoir operations affected the local geological environment, downstream riverbed erosion, evolution of the Yellow River delta, water quality, and aquatic biodiversity. Lessons from the impact assessment of the Xiaolangdi Reservoir are summarized as follows: (1) The construction of large reservoirs is not merely an engineering challenge but must also be viewed in terms of resource exploitation, environmental protection, and social development; (2) long-term systems for monitoring large reservoirs should be established, and decision makers involved at national policy and planning levels must be prepared to react quickly to the changing impact of large reservoirs; and (3) the key to solving sedimentation in the LYR is not Xiaolangdi Reservoir but instead soil conservation in the middle reaches of the Yellow River basin. Proper assessment of the impacts of large reservoirs will help promote development strategies that enhance the long-term sustainability of dam projects.
Is it time for a new yellow fever vaccine?

PubMed

Hayes, Edward B

2010-11-29

An inexpensive live attenuated vaccine (the 17D vaccine) against yellow fever has been effectively used to prevent yellow fever for more than 70 years. Interest in developing new inactivated vaccines has been spurred by recognition of rare but serious, sometimes fatal adverse events following live virus vaccination. A safer inactivated yellow fever vaccine could be useful for vaccinating people at higher risk of adverse events from the live vaccine, but could also have broader global health utility by lowering the risk-benefit threshold for assuring high levels of yellow fever vaccine coverage. If ongoing trials demonstrate favorable immunogenicity and safety compared to the current vaccine, the practical global health utility of an inactivated vaccine is likely to be determined mostly by cost. Copyright © 2010 Elsevier Ltd. All rights reserved.
THE USE OF MICE IN TESTS OF IMMUNITY AGAINST YELLOW FEVER

PubMed Central

Sawyer, W. A.; Lloyd, Wray

1931-01-01

1. A method of testing sera for protective power against yellow fever is described and designated as the intraperitoneal protection test in mice. 2. The test consists essentially of the inoculation of mice intra-peritoneally with yellow fever virus, fixed for mice, together with the serum to be tested, and the simultaneous injection of starch solution into the brain to localize the virus. If the serum lacks protective power the mice die of yellow fever encephalitis. 3. The test is highly sensitive. Consequently it is useful in epidemiological studies to determine whether individuals have ever had yellow fever and in tests to find whether vaccinated persons or animals have in reality been immunized. 4. When mice were given large intraperitoneal injections of yellow fever virus fixed for mice, the virus could be recovered from the blood for 4 days although encephalitis did not occur. If the brain was mildly injured at the time of the intraperitoneal injection, the symptoms of yellow fever encephalitis appeared 6 days later, but the virus was then absent from the blood. 5. Strains of white mice vary greatly in their susceptibility to yellow fever. PMID:19869938
A Case of Yellow Fever Vaccine–Associated Viscerotropic Disease in Ecuador

PubMed Central

Douce, Richard W.; Freire, Diana; Tello, Betzabe; Vásquez, Gavino A.

2010-01-01

We report the first case of viscerotropic syndrome in Ecuador. Because of similarities between yellow fever and viscerotropic syndrome, the incidence of this recently described complication of vaccination with the 17D yellow fever vaccine is not known. There is a large population in South America that is considered at risk for possible reemergence of urban yellow fever. Knowledge of potentially fatal complications of yellow fever vaccine should temper decisions to vaccinate populations where the disease is not endemic. PMID:20348528
Yellow River Delta, China

NASA Image and Video Library

2009-12-08

The Yellow River is the second-longest river in China, and the sixth longest in the world and makes many dramatic shifts over time. This image was taken with the ASTER instrument aboard NASA Terra spacecraft in 2009.
Yellow-Poplar Rooting Habits

Treesearch

John K. Francis

1979-01-01

Although the configuration of pole-sized yellow-poplar root systems in Tennessee is quite variable, a branched taproot with several widely spreading laterals is typical. Rooting depth is particularly limited by clayey texture, wetness, and firmness of subsoils.
Seasonal differences in freezing tolerance of yellow-cedar and western hemlock trees at a site affected by yellow-cedar decline

Treesearch

Paul G. Schaberg; Paul E. Hennon; Amore, David V. D; Gary J. Hawley; Catherine H. Borer; Catherine H. Borer

2005-01-01

To assess whether inadequate cold hardiness could be a contributor to yellow-cedar (Chamaecyparis nootkatensis (D. Don) Spach) decline, we measured the freezing tolerance of foliage from yellow-cedar trees in closed-canopy (nondeclining) and open-canopy (declining at elevations below 130 m) stands at three sites along an elevational gradient in the heart of the decline...
Historic and contemporary mercury exposure and potential risk to yellow-billed loons (Gavia adamsii) breeding in Alaska and Canada

USGS Publications Warehouse

Evers, David C.; Schmutz, Joel A.; Basu, Niladri; DeSorbo, Christopher R.; Fair, Jeff; Gray, Carrie E.; Paruk, James D.; Perkins, Marie; Regan, Kevin; Uher-Koch, Brian D.; Wright, Kenneth G.

2014-01-01

The Yellow-billed Loon (Gavia adamsii) is one of the rarest breeding birds in North America. Because of the small population size and patchy distribution, any stressor to its population is of concern. To determine risks posed by environmental mercury (Hg) loads, we captured 115 Yellow-billed Loons between 2002 and 2012 in the North American Arctic and sampled their blood and/or feather tissues and collected nine eggs. Museum samples from Yellow-billed Loons also were analyzed to examine potential changes in Hg exposure over time. An extensive database of published Hg concentrations and associated adverse effects in Common Loons (G. immer) is highly informative and representative for Yellow-billed Loons. Blood Hg concentrations reflect dietary uptake of methylmercury (MeHg) from breeding areas and are generally considered near background levels if less than 1.0 µg/g wet weight (ww). Feather (grown at wintering sites) and egg Hg concentrations can represent a mix of breeding and wintering dietary uptake of MeHg. Based on Common Loon studies, significant risk of reduced reproductive success generally occurs when adult Hg concentrations exceed 2.0 µg/g ww in blood, 20.0 µg/g fresh weight (fw) in flight feathers and 1.0 µg/g ww in eggs. Contemporary mercury concentrations for 176 total samples (across all study sites for 115 Yellow-billed Loons) ranged from 0.08 to 1.45 µg/g ww in blood, 3.0 to 24.9 µg/g fw in feathers and 0.21 to 1.23 µg/g ww in eggs. Mercury concentrations in blood, feather and egg tissues indicate that some individual Yellow-billed Loons in breeding populations across North America are at risk of lowered productivity resulting from Hg exposure. Most Yellow-billed Loons breeding in Alaska overwinter in marine waters of eastern Asia. Although blood Hg concentrations from most breeding loons in Alaska are within background levels, some individuals exhibit elevated feather and egg Hg concentrations, which likely indicate the uptake of Me
Introducing the yellow laser

NASA Astrophysics Data System (ADS)

Lincoln, James

2018-02-01

The author has acquired a yellow laser with the specific wavelength of 589 nm. Because this is the first time such a laser has been discussed in this journal, I feel it is appropriate to provide a discussion of its function and capabilities. Normal laser safety should be employed, such as not pointing it into eyes or at people, and using eye protection for the young and inexperienced. It is important to note that 589 nm is the same wavelength as the Sodium-D line (doublet). This allows for the laser to serve as a replacement for sodium lamps, and, considering its rather high price, this added value should be balanced against its cost. What follows is a list of activities that showcase the yellow laser's unique promise as an engaging piece of technology that can be used in the teaching of physics.
Impact of yellow fever on the developing world.

PubMed

Tomori, O

1999-01-01

Yellow fever (YF) has remained a disease of public health importance since it was first described in the fifteenth century. At different periods in human history, YF has caused untold hardship and indescribable misery among populations in the Americas, Europe, and Africa. It brought economic disaster in its wake, constituting a stumbling block to development. Yellow fever is an arboviral infection with three epidemiological transmission cycles between monkeys, mosquitoes, and humans. It is an acute infectious disease characterized by sudden onset, with two phases of development separated by a short period of remission. The clinical spectrum of YF varies from a very mild, nonspecific, febrile illness to a fulminating, sometimes fatal disease with pathognomonic features. In severe cases, jaundice and bleeding diathesis with hepatorenal involvement are common. The fatality rate of severe YF is 50% or higher. Despite landmark achievements in the understanding of the epidemiology of YF and the availability of a safe, efficacious vaccine, YF remains a major public health problem in both Africa and South America, where annually the disease affects an estimated 200,000 persons, causing an estimated 30,000 deaths. Since the 1980s epidemics of YF in Africa have affected predominantly children under the age of 15 years. The failure to control YF arises from a misapplication of public health strategies and insufficient political commitment by governments in YF endemic areas, especially in Africa, to control the disease.
A Novel Benzodiazepine Compound Inhibits Yellow Fever Virus Infection by Specifically Targeting NS4B Protein.

PubMed

Guo, Fang; Wu, Shuo; Julander, Justin; Ma, Julia; Zhang, Xuexiang; Kulp, John; Cuconati, Andrea; Block, Timothy M; Du, Yanming; Guo, Ju-Tao; Chang, Jinhong

2016-09-21

-risk regions. It has been estimated that up to 1.7 million YFV infections occur in Africa each year, resulting in 29,000 to 60,000 death. Thus far, there is no specific antiviral treatment for yellow fever. To cope with this medical challenge, we identified a benzodiazepine compound that selectively inhibits YFV by targeting the viral NS4B protein. To our knowledge, this is the first report demonstrating in vivo safety and antiviral efficacy of an YFV NS4B inhibitor in an animal model. We have thus reached a critical milestone toward the development of specific antiviral therapeutics for clinical management of yellow fever. Copyright © 2016, American Society for Microbiology. All Rights Reserved.
Silvical characteristics of yellow birch (Betula alleghaniensis)

Treesearch

Adrian M. Gilbert

1960-01-01

Of the birches in the Northeast, the yellow birch is the elite species, by far the most valuable as a timber tree. More than that, it is one of the largest deciduous trees of northeastern America. It may reach 100 feet in height and more than 3 feet in diameter, and may live to 300 years of age. Pioneers told tales of the gigantic yellow birches they saw.
Travelers' Health: Yellow Fever

MedlinePlus

... and local rate of virus transmission at the time of travel. Although reported cases of human disease are the ... be receiving yellow fever vaccine for the first time. If travel is unavoidable, the decision to vaccinate travelers aged ≥ ...
Petroleum hydrocarbons in a water-sediment system from Yellow River estuary and adjacent coastal area, China: Distribution pattern, risk assessment and sources.

PubMed

Wang, Min; Wang, Chuanyuan; Li, Yuanwei

2017-09-15

Aliphatic hydrocarbons (AHs), biomarker and polycyclic aromatic hydrocarbons (PAHs) concentrations of surface water and sediment samples collected from Yellow River Estuary and adjacent coastal area in China were measured to determine their spatial distributions, analyze their sources and evaluate the ecological risk of PAHs in the water-sediment system. The spatial distributions of n-alkane in sediments are mainly controlled by the mixing inputs of terrigenous and marine components. In comparison with AHs, the total concentrations of Σ16PAHs in surface sediments from a transect of the offshore area were noticeably higher than that of the riverine and estuary areas. Additionally, the AHs and total PAHs concentrations all indicated an overall pattern of a seaward decrease. The PAHs concentrations during the dry season (mainly in the form of dissolved phase) were higher than that of PAHs (mainly dissolved phase and particulate phase form) in the flooding season. In comparison with global concentration levels of PAHs, the level of PAHs in suspended particulate matter and sediments from the Yellow River Estuary was lower than those from other countries, while the concentration of PAHs in the dissolved phase were in the middle range. Petroleum contamination, mainly from oil exploration and discharge of pollutants from rivers, was the main source of n-alkanes. The PAHs in the river were mostly of petrogenic origin, while those in the estuarial and marine areas originated mainly from pyrogenic sources. The results of the toxicology assessment suggested that the PAHs in sediments from Yellow River Estuary and adjacent coastal area exhibited a low potential eco-toxicological contamination level. Copyright © 2017 Elsevier Ltd. All rights reserved.
[The fourth horseman: The yellow fever].

PubMed

Vallejos-Parás, Alfonso; Cabrera-Gaytán, David Alejandro

2017-01-01

Dengue virus three, Chikunguya and Zika have entered the national territory through the south of the country. Cases and outbreaks of yellow fever have now been identified in the Americas where it threatens to expand. Although Mexico has a robust epidemiological surveillance system for vector-borne diseases, our country must be alert in case of its possible introduction into the national territory. This paper presents theoretical assumptions based on factual data on the behavior of yellow fever in the Americas, as well as reflections on the epidemiological surveillance of vector-borne diseases.
Yellow fever: a reemerging threat.

PubMed

Gardner, Christina L; Ryman, Kate D

2010-03-01

Yellow fever (YF) is a viral disease, endemic to tropical regions of Africa and the Americas, which principally affects humans and nonhuman primates and is transmitted via the bite of infected mosquitoes. Yellow fever virus (YFV) can cause devastating epidemics of potentially fatal, hemorrhagic disease. Despite mass vaccination campaigns to prevent and control these outbreaks, the risk of major YF epidemics, especially in densely populated, poor urban settings, both in Africa and South America, has greatly increased. Consequently, YF is considered an emerging, or reemerging disease of considerable importance. This article comprehensively reviews the history, microbiology, epidemiology, clinical presentation, diagnosis, and treatment of YFV, as well as the vaccines produced to combat YF. 2010 Elsevier Inc. All rights reserved.
Cucumber vein yellowing virus

USDA-ARS?s Scientific Manuscript database

Cucurbits are an important crop of temperate, subtropical and tropical regions of the world. Cucumber vein yellowing virus (CVYV) is a major viral pathogen of cucurbits. This chapter provides an overview of the biology of CVYV and the disease it causes....
Yellowing and bleaching of grey hair caused by photo and thermal degradation.

PubMed

Richena, M; Silveira, M; Rezende, C A; Joekes, I

2014-09-05

Yellowing is an undesirable phenomenon that is common in people with white and grey hair. Because white hair has no melanin, the pigment responsible for hair colour, the effects of photodegradation are more visible in this type of hair. The origin of yellowing and its relation to photodegradation processes are not properly established, and many questions remain open in this field. In this work, the photodegradation of grey hair was investigated as a function of the wavelength of incident radiation, and its ultrastructure was determined, always comparing the results obtained for the white and black fibres present in grey hair with the results of white wool. The results presented herein indicate that the photobehaviour of grey hair irradiated with a mercury lamp or with solar radiation is dependent on the wavelength range of the incident radiation and on the initial shade of yellow in the sample. Two types of grey hair were used: (1) blended grey hair (more yellow) and (2) grey hair from a single-donor (less yellow). After exposure to a full-spectrum mercury lamp for 200 h, the blended white hair turned less yellow (the yellow-blue difference, Db(*) becomes negative, Db(*)=-6), whereas the white hair from the single-donor turned slightly yellower (Db(*)=2). In contrast, VIS+IR irradiation resulted in bleaching in both types of hair, whereas a thermal treatment (at 81 °C) caused yellowing of both types of hair, resulting in a Db(*)=3 for blended white hair and Db(*)=9 for single-donor hair. The identity of the yellow chromophores was investigated by UV-Vis spectroscopy. The results obtained with this technique were contradictory, however, and it was not possible to obtain a simple correlation between the sample shade of yellow and the absorption spectra. In addition, the results are discussed in terms of the morphology differences between the pigmented and non-pigmented parts of grey hair, the yellowing and bleaching effects of grey hair, and the occurrence of dark
Population dynamics of American horseshoe crabs-historic climatic events and recent anthropogenic pressures

USGS Publications Warehouse

Faurby, S.; King, T.L.; Obst, M.; Hallerman, E.M.; Pertoldi, C.; Funch, P.

2010-01-01

Populations of the American horseshoe crab, Limulus polyphemus, have declined, but neither the causes nor the magnitude are fully understood. In order to evaluate historic demography, variation at 12 microsatellite DNA loci surveyed in 1218 L. polyphemus sampled from 28 localities was analysed with Bayesian coalescent-based methods. The analysis showed strong declines in population sizes throughout the species' distribution except in the geographically isolated southern-most population in Mexico, where a strong increase in population size was inferred. Analyses suggested that demographic changes in the core of the distribution occurred in association with the recolonization after the Ice Age and also by anthropogenic effects, such as the past overharvest of the species for fertilizer or the current use of the animals as bait for American eel (Anguilla rostrata) and whelk (Busycon spp.) fisheries. This study highlights the importance of considering both climatic changes and anthropogenic effects in efforts to understand population dynamics-a topic which is highly relevant in the ongoing assessments of the effects of climate change and overharvest. ?? 2010 Blackwell Publishing Ltd.

Population dynamics of American horseshoe crabs--historic climatic events and recent anthropogenic pressures.

PubMed

Faurby, Søren; King, Tim L; Obst, Matthias; Hallerman, Eric M; Pertoldi, Cino; Funch, Peter

2010-08-01

Populations of the American horseshoe crab, Limulus polyphemus, have declined, but neither the causes nor the magnitude are fully understood. In order to evaluate historic demography, variation at 12 microsatellite DNA loci surveyed in 1218 L. polyphemus sampled from 28 localities was analysed with Bayesian coalescent-based methods. The analysis showed strong declines in population sizes throughout the species' distribution except in the geographically isolated southern-most population in Mexico, where a strong increase in population size was inferred. Analyses suggested that demographic changes in the core of the distribution occurred in association with the recolonization after the Ice Age and also by anthropogenic effects, such as the past overharvest of the species for fertilizer or the current use of the animals as bait for American eel (Anguilla rostrata) and whelk (Busycon spp.) fisheries. This study highlights the importance of considering both climatic changes and anthropogenic effects in efforts to understand population dynamics--a topic which is highly relevant in the ongoing assessments of the effects of climate change and overharvest.
What a rheumatologist needs to know about yellow fever vaccine.

PubMed

Oliveira, Ana Cristina Vanderley; Mota, Licia Maria Henrique da; Santos-Neto, Leopoldo Luiz Dos; Tauil, Pedro Luiz

2013-04-01

Patients with rheumatic diseases are more susceptible to infection, due to the underlying disease itself or to its treatment. The rheumatologist should prevent infections in those patients, vaccination being one preventive measure to be adopted. Yellow fever is one of such infectious diseases that can be avoided.The yellow fever vaccine is safe and effective for the general population, but, being an attenuated live virus vaccine, it should be avoided whenever possible in rheumatic patients on immunosuppressive drugs. Considering that yellow fever is endemic in a large area of Brazil, and that vaccination against that disease is indicated for those living in such area or travelling there, rheumatologists need to know that disease, as well as the indications for the yellow fever vaccine and contraindications to it. Our paper was aimed at highlighting the major aspects rheumatologists need to know about the yellow fever vaccine to decide about its indication or contraindication in specific situations. 2013 Elsevier Editora Ltda. All rights reserved.
Yellow Fever Outbreaks in Unvaccinated Populations, Brazil, 2008–2009

PubMed Central

Romano, Alessandro Pecego Martins; Costa, Zouraide Guerra Antunes; Ramos, Daniel Garkauskas; Andrade, Maria Auxiliadora; Jayme, Valéria de Sá; de Almeida, Marco Antônio Barreto; Vettorello, Kátia Campomar; Mascheretti, Melissa; Flannery, Brendan

2014-01-01

Due to the risk of severe vaccine-associated adverse events, yellow fever vaccination in Brazil is only recommended in areas considered at risk for disease. From September 2008 through June 2009, two outbreaks of yellow fever in previously unvaccinated populations resulted in 21 confirmed cases with 9 deaths (case-fatality, 43%) in the southern state of Rio Grande do Sul and 28 cases with 11 deaths (39%) in Sao Paulo state. Epizootic deaths of non-human primates were reported before and during the outbreak. Over 5.5 million doses of yellow fever vaccine were administered in the two most affected states. Vaccine-associated adverse events were associated with six deaths due to acute viscerotropic disease (0.8 deaths per million doses administered) and 45 cases of acute neurotropic disease (5.6 per million doses administered). Yellow fever vaccine recommendations were revised to include areas in Brazil previously not considered at risk for yellow fever. PMID:24625634
Survival and growth of Cochlodinium polykrikoides red tide after addition of yellow loess.

PubMed

Lee, Young Sik; Kim, Jung Dong; Lim, Weol Ae; Lee, Sam Geun

2009-11-01

We examined the survival rate of Cochlodinium polykrikoides after yellow loess addition and conducted culture experiments to investigate the possibility that red tides maybe caused by C. polykrikoides individuals that are precipitated when loess is added. At least 15% of the C. polykrikoides cells that precipitated to the bottom layer either by the addition of loess or no addition survived for 1 week at all growth phases, rather than disappearing immediately after precipitating. However no live cells were observed after 20 days, regardless of phase or loess addition. In the exponential phase, the number of C. polykrikoides cells increased to >2886 cells ml(-1) after loess was added. However in the stationary phase, the number of cells did not increase until 18 days. In the exponential phase, those C. polykrikoides that survived precipitation caused by scattering loess on cultures did not appear to have the ability to cause red tides again because of the short red tide periods in the field, long lag time after loess addition, and low survival rate after loess addition.
Acetylcholine, ATP, and proteoglycan are common to synaptic vesicles isolated from the electric organs of electric eel and electric catfish as well as from rat diaphragm.

PubMed

Volknandt, W; Zimmermann, H

1986-11-01

Cholinergic synaptic vesicles were isolated from the electric organs of the electric eel (Electrophorus electricus) and the electric catfish (Malapterurus electricus) as well as from the diaphragm of the rat by density gradient centrifugation followed by column chromatography on Sephacryl-1000. This was verified by both biochemical and electron microscopic criteria. Differences in size between synaptic vesicles from the various tissue sources were reflected by their elution pattern from the Sephacryl column. Specific activities of acetylcholine (ACh; in nmol/mg of protein) of chromatography-purified vesicle fractions were 36 (electric eel), 2 (electric catfish), and 1 (rat diaphragm). Synaptic vesicles from all three sources contained ATP in addition to ACh (molar ratios of ACh/ATP, 9-12) as well as binding activity for an antibody raised against Torpedo cholinergic synaptic vesicle proteoglycan. Synaptic vesicles from rat diaphragm contained binding activity for the monoclonal antibody asv 48 raised against a rat brain 65-kilodalton synaptic vesicle protein. Antibody asv 48 binding was absent from electric eel and electric catfish synaptic vesicles. These antibody binding results, which were obtained by a dot blot assay on isolated vesicles, directly correspond to the immunocytochemical results demonstrating fluorescein isothiocyanate staining in the respective nerve terminals. Our results imply that ACh, ATP, and proteoglycan are common molecular constituents of motor nerve terminal-derived synaptic vesicles from Torpedo to rat. In addition to ACh, both ATP and proteoglycan may play a specific role in the process of cholinergic signal transmission.
Serious adverse events associated with yellow fever vaccine.

PubMed

de Menezes Martins, Reinaldo; Fernandes Leal, Maria da Luz; Homma, Akira

2015-01-01

Yellow fever vaccine was considered one of the safest vaccines, but in recent years it was found that it could rarely cause invasive and disseminated disease in some otherwise healthy individuals, with high lethality. After extensive studies, although some risk factors have been identified, the real cause of causes of this serious adverse event are largely unknown, but findings point to individual host factors. Meningoencephalitis, once considered to happen only in children less than 6 months of age, has also been identified in older children and adults, but with good prognosis. Efforts are being made to develop a safer yellow fever vaccine, and an inactivated vaccine or a vaccine prepared with the vaccine virus envelope produced in plants are being tested. Even with serious and rare adverse events, yellow fever vaccine is the best way to avoid yellow fever, a disease of high lethality and should be used routinely in endemic areas, and on people from non-endemic areas that could be exposed, according to a careful risk-benefit analysis.
THE SUSCEPTIBILITY OF MARMOSETS TO YELLOW FEVER VIRUS

PubMed Central

Davis, Nelson C.

1930-01-01

1. It has been possible to introduce yellow fever virus into the small Brazilian monkeys, Callithrix albicollis and Leontocebus ursulus, by the bites of infected mosquitoes and to carry the virus through a series of four passages in each species and back to rhesus monkeys by the bites of Stegomyia mosquitoes fed on the last marmoset of each series. 2. Five specimens of L. ursulus were used. Four developed fever, and all died during the experiments. At least two showed liver necroses comparable to those found in human beings and rhesus monkeys that died of yellow fever. 3. Twenty specimens of C. albicollis were used. Very few showed a temperature reaction following the introduction of virus. Of those that died, none had lesions typical of yellow fever as seen in certain other species of monkeys and in humans. 4. The convalescent serum from each of five C. albicollis protected a rhesus monkey against yellow fever virus, but the serum from a normal marmoset of the same species was found to be non-protective. PMID:19869773
Identification and characterization of the intermediate phase in hybrid organic-inorganic MAPbI3 perovskite.

PubMed

Guo, Xin; McCleese, Christopher; Kolodziej, Charles; Samia, Anna C S; Zhao, Yixin; Burda, Clemens

2016-03-07

Perovskite films were prepared using single step solution deposition at different annealing temperatures and annealing times. The crystal structure, phases and grain size were investigated with XRD, XPS and SEM/EDX. The prepared films show a typical orientation of tetragonal perovskite phase and a gradual transition at room temperature from the yellow intermediate phase to the black perovskite phase. Films with high purity were obtained by sintering at 100 °C. In addition, the chemical composition and crystal structure of intermediate phase were investigated in detail. FTIR, UV-vis and NMR spectra revealed the occurance of DMF complexes. Interestingly, the intermediate phase could be transformed to the black perovskite phase upon X-ray irradiation. In addition, the recovery of the aged perovskite films from a yellow intermediate phase back to the black perovskite was shown to be viable via heating and X-ray irradiation.
Squash vein yellowing virus

USDA-ARS?s Scientific Manuscript database

Cucurbits are an important crop of temperate, subtropical and tropical regions of the world. Squash vein yellowing virus (SqVYV) is a major viral pathogen of cucurbits. This chapter provides an overview of the biology of SqVYV and the disease it causes....
Traveling Abroad: Latest Yellow Fever Vaccine Update | Poster

Cancer.gov

Earlier this month, the U.S. Centers for Disease Control and Prevention (CDC) released its list of clinics that are administering the yellow fever vaccine Stamaril, which has been made available to address the total depletion of the United States’ primary yellow fever vaccine, YF-VAX. These clinics will provide the vaccine to individuals preparing for international travel,
Yellow Fever in an Unvaccinated Traveler to Peru.

PubMed

Winnicka, Lydia; Abdullah, Amirahwaty; Yang, Tsujung; Norville, Kim; Irizarry-Acosta, Melina

2017-01-01

We present a case of an unvaccinated traveler who traveled from New York to Peru and contracted yellow fever. He likely acquired the infection while visiting the Amazon River, with a point of exit of Lima, Peru. Our case illustrates the dramatic course that yellow fever may take, as well as the importance of pretravel vaccination.
A Matter of Contrast: Yellow Flower Colour Constrains Style Length in Crocus species.

PubMed

Lunau, Klaus; Konzmann, Sabine; Bossems, Jessica; Harpke, Doerte

2016-01-01

Most flowers display distinct colour patterns comprising two different areas. The peripheral large-area component of floral colour patterns attracts flower visitors from some distance and the central small-area component guides flower visitors towards landing sites. Whereas the peripheral colour is largely variable among species, the central colour, produced mostly by anthers and pollen or pollen mimicking floral guides, is predominantly yellow and UV-absorbing. This holds also for yellow flowers that regularly display a UV bull's eye pattern. Here we show that yellow-flowering Crocus species are a noticeable exception, since yellow-flowering Crocus species-being entirely UV-absorbing-exhibit low colour contrast between yellow reproductive organs and yellow tepals. The elongated yellow or orange-yellow style of Crocus flowers is a stamen-mimicking structure promoting cross-pollination by facilitating flower visitors' contact with the apical stigma before the flower visitors are touching the anthers. Since Crocus species possess either yellow, violet or white tepals, the colour contrast between the stamen-mimicking style and the tepals varies among species. In this study comprising 106 Crocus species, it was tested whether the style length of Crocus flowers is dependent on the corolla colour. The results show that members of the genus Crocus with yellow tepals have evolved independently up to twelve times in the genus Crocus and that yellow-flowering Crocus species possess shorter styles as compared to violet- and white-flowering ones. The manipulation of flower visitors by anther-mimicking elongated styles in Crocus flowers is discussed.
[Poison and the mosquito: epistemological aspects of the etiology and prophylactics of yellow fever].

PubMed

Caponi, S

2000-01-01

The strategies against yellow fever developed by Argentina and Brazil were discussed at the Second Medical Congress of Latin America which was held in Buenos Aires in 1904. The study of the controversy between physicians from Argentina and Brazil around the existing explanatory models of this illness and the international prophylactic strategies in use at the time enables an epistemological understanding of the breakthrough brought about by the emergence of medicine of vectors. This chapter of Latin American medicine history constitutes a unique opportunity to analyze that reorganization of knowledge, which permitted the inclusion of intermediary living beings into the medical and epidemiological discourse.
Pseudocapillaria (Ichthyocapillaria) ophisterni sp. n. (Nematoda : Capillariidae) from the swamp-eel Ophisternon aenigmaticum (Pisces) in Mexico.

PubMed

Moravec, F; Salgado-Maldonado, G; Jiménez-García, I

2000-04-01

A new nematode species, Pseudocapillaria ophisterni sp. n., is described from the intestine and rarely from the stomach of the swamp-eel, Ophisternon aenigmaticum Rosen et Greenwood, from Catemaco Lake, Veracruz, Mexico. In having both caudal lobes in the male interconnected by a distinct dorsal membrane, it belongs to the subgenus Ichthyocapillaria. It differs from the three species in this subgenus mainly in possessing either a distinctly longer spicule or a smaller length of oesophagus relative to body length. It also differs in host type and geographical distribution. P. ophisterni is the first capillariid species reported from synbranchiform fishes.
Transmission of yellow fever vaccine virus through breast-feeding - Brazil, 2009.

PubMed

2010-02-12

In April, 2009, the state health department of Rio Grande do Sul, Brazil, was notified by the Cachoeira do Sul municipal health department of a case of meningoencephalitis requiring hospitalization in an infant whose mother recently had received yellow fever vaccine during a postpartum visit. The Field Epidemiology Training Program of the Secretariat of Surveillance in Health of the Brazilian Ministry of Health assisted state and municipal health departments with an investigation. This report summarizes the results of that investigation, which determined that the infant acquired yellow fever vaccine virus through breast-feeding. The mother reported 2 days of headache, malaise, and low fever occurring 5 days after receipt of yellow fever vaccine. The infant, who was exclusively breast-fed, was hospitalized at age 23 days with seizures requiring continuous infusion of intravenous anticonvulsants. The infant received antimicrobial and antiviral treatment for meningoencephalitis. The presence of 17DD yellow fever virus was detected by reverse transcription--polymerase chain reaction (RT-PCR) in the infant's cerebrospinal fluid (CSF); yellow fever--specific immunoglobulin M (IgM) antibodies also were present in serum and CSF. The infant recovered completely, was discharged after 24 days of hospitalization, and has had normal neurodevelopment and growth through age 6 months. The findings in this report provide documentation that yellow fever vaccine virus can be transmitted via breast-feeding. Administration of yellow fever vaccine to breast-feeding women should be avoided except in situations where exposure to yellow fever viruses cannot be avoided or postponed.
Increasing use of yellow colors in Kyoto

NASA Astrophysics Data System (ADS)

Akita, Munehira; Nara, Iwao

2002-06-01

Colors used for commercial signboards, displayed outdoors as well as indoors through windows, such as a store sign, an advertising sign, a sky sign, a poster, a placard, and a billboard were extensively surveyed in Kyoto City, Japan, in 1998. The survey showed that various kinds of yellow painted signs have increased rapidly and invaded a center area and suburbs of the city. Vivid yellow, what we called it the Y98 virus, is specially considered a color unpleasantly matched to the city image of Kyoto which was the capital of Japan for nearly 1000 years (794 to 1868) and is endowed with cultural and historic heritage. Discussions trying to find out what we could do to prevent the rapid spread of a big commercial display painted with vivid yellows what we called 'the Y98 virus' over the city will be summarized in a main text.
Barley yellow dwarf virus: Luteoviridae or Tombusviridae?

PubMed

Miller, W Allen; Liu, Sijun; Beckett, Randy

2002-07-01

Summary Barley yellow dwarf virus (BYDV), the most economically important virus of small grains, features highly specialised relationships with its aphid vectors, a plethora of novel translation mechanisms mediated by long-distance RNA interactions, and an ambiguous taxonomic status. The structural and movement proteins of BYDV that confer aphid transmission and phloem-limitation properties resemble those of the Luteoviridae, the family in which BYDV is classified. In contrast, many genes and cis-acting signals involved in replication and gene expression most closely resemble those of the Tombusviridae. BYDV is in genus Luteovirus, family Luteoviridae. BYDV includes at least two serotypes or viruses: BYDV-PAV and BYDV-MAV. The former BYDV-RPV is now Cereal yellow dwarf virus-RPV (CYDV-RPV). CYDV is in genus Polerovirus, family Luteoviridae. Genus Luteovirus shares many features with family Tombusviridae. Physical properties: approximately 25 nm icosahedral (T = 3) virions. One major (22 kDa) and one minor (50-55 kDa) coat protein. 5.6-5.8 kb positive sense RNA genome with no 5'-cap and no poly(A) tail. Most grasses. Most important in oats, barley and wheat. Also infects maize and rice. Yellowing and dwarfing in barley, stunting in wheat; reddening, yellowing and blasting in oats. Some isolates cause leaf notching and curling. Key attractions: Model for the study of circulative transmission of aphid-transmitted viruses. Plethora of unusual translation mechanisms. Evidence of recombination in recent evolutionary history creates taxonomic ambiguity. Economically important virus of wheat, barley and oats, worldwide. Useful websites/meetings: International symposium: 'Barley Yellow Dwarf Disease: Recent Advances and Future Strategies', CIMMYT, El Batan, Mexico, 1-5 September 2002, http://www.cimmyt.cgiar.org/Research/wheat/Conf_BYD_02/invitation.htm http://www.cimmyt.org/Research/wheat/BYDVNEWS/htm/BYDVNEWS.htm Aphid transmission animation: http://www.ppws.vt.edu/~sforza/tmv/bydv_aph.html.
Prototype Scale Development of an Environmentally Benign Yellow Smoke Hand-Held Signal Formulation Based on Solvent Yellow 33

DTIC Science & Technology

2013-04-15

VAAR) was purchased from McGean. Hydrated basic magnesium carbonate was obtained from Pine Bluff Arsenal (Pine Bluff, AR) and was confirmed to be Mg5( CO3 ...a potential environmental exposure of approximately 29 g of the toxic yellow dyes throughout the life cycle of a single M194 signal! To mitigate this...consists of Solvent Yellow 33 as the smoke sublimating agent, hydrated basic magnesium carbonate (Mg5( CO3 )4(OH)2·4H2O) instead of sodium bicarbonate (NaHCO3
Water Quality Criteria for Colored Smokes: Solvent Yellow 33

DTIC Science & Technology

1987-11-01

Y . ’~ ~% d .’ 4’ . TABLE 4. DISTRIBUTION OF [1 4 C]-SOLVENT YELLOW 33 IN RATS 1 hr AFTER- EXPOSURE TO SOLVENT YELLOW 33 (SY) OR SOLVENT YELLOW 33...have shown that some individuals react strongly Lo this dye. The repeat insult patch test is used most often. The subjects receive five to ten exposures...70 Neutrophils Control 5 ± 2 0 ± 0 7 ± 3 3( lO cells/g) Exposed 1300 ± 130 d 470 ± i 0 0d 290 ± 50 d a. Adapted from Henderson et al. 1985b. b. Values
Yellow fever, Asia and the East African slave trade.

PubMed

Cathey, John T; Marr, John S

2014-05-01

Yellow fever is endemic in parts of sub-Saharan Africa and South America, yet its principal vectors--species of mosquito of the genus Aedes--are found throughout tropical and subtropical latitudes. Phylogenetic analyses indicate that yellow fever originated in Africa and that its spread to the New World coincided with the slave trade, but why yellow fever has never appeared in Asia remains a mystery. None of several previously proposed explanations for its absence there is considered satisfactory. We contrast the trans-Atlantic slave trade, and trade across the Sahara and to the Arabian Peninsula and Mesopotamia, with that to Far East and Southeast Asian ports before abolition of the African slave trade, and before the scientific community understood the transmission vector of yellow fever and the viral life cycle, and the need for shipboard mosquito control. We propose that these differences in slave trading had a primary role in the avoidance of yellow fever transmission into Asia in the centuries before the 20(th) century. The relatively small volume of the Black African slave trade between Africa and East and Southeast Asia has heretofore been largely ignored. Although focal epidemics may have occurred, the volume was insufficient to reach the threshold for endemicity.

Bedding Improves Yellow-Poplar Growth on Fragipan Soils

Treesearch

John K. Francis

1979-01-01

Yellow-poplar can be grown on soils that have a shallow fragipan--but unless such sites are bedded, growth is likely to be extremely poor. In a Tennessee study, bedding increased height of planted yellow-poplar over 5 years, but fertilizer did not. Because of the cost of bedding and the availability of nonfragipan sites, it would ordinarily be better not to plant...
Western yellow pine in Arizona and New Mexico

Treesearch

Theodore S. Woolsey

1911-01-01

Western yellow pine is to the Southwest what white pine is to the Northeast, or longleaf pine to the Southeast. The commercial forests of Arizona and New Mexico are three-fourths western yellow pine, which furnishes by far the greater part of the lumber used locally as well as that shipped to outside markets. To describe the characteristics of the species and to...
Insights Into the Etiology of Polerovirus-Induced Pepper Yellows Disease.

PubMed

Lotos, Leonidas; Olmos, Antonio; Orfanidou, Chrysoula; Efthimiou, Konstantinos; Avgelis, Apostolos; Katis, Nikolaos I; Maliogka, Varvara I

2017-12-01

The study of an emerging yellows disease of pepper crops (pepper yellows disease [PYD]) in Greece led to the identification of a polerovirus closely related to Pepper vein yellows virus (PeVYV). Recovery of its full genome sequence by next-generation sequencing of small interfering RNAs allowed its characterization as a new poleroviruses, which was provisionally named Pepper yellows virus (PeYV). Transmission experiments revealed its association with the disease. Sequence similarity and phylogenetic analysis highlighted the common ancestry of the three poleroviruses (PeVYV, PeYV, and Pepper yellow leaf curl virus [PYLCV]) currently reported to be associated with PYD, even though significant genetic differences were identified among them, especially in the C-terminal region of P5 and the 3' noncoding region. Most of the differences observed can be attributed to a modular type of evolution, which produces mosaic-like variants giving rise to these different poleroviruses Overall, similar to other polerovirus-related diseases, PYD is caused by at least three species (PeVYV, PeYV, and PYLCV) belonging to this group of closely related pepper-infecting viruses.
[A novel yellow organic light-emitting device].

PubMed

Ma, Chen; Wang, Hua; Hao, Yu-Ying; Gao, Zhi-Xiang; Zhou, He-Feng; Xu, Bing-She

2008-07-01

The fabrication of a novel organic yellow-light-emitting device using Rhodamine B as dopant with double quantum-well (DQW) structure was introduced in the present article. The structure and thickness of this device is ITO/CuPc (6 nm) /NPB (20 nm) /Alq3 (3 nm)/Alq3 : Rhodamine B (3 nm) /Alq3 (3 nm) /Al q3 : Rhodamine B(3 nm) /Alq3 (30 nm) /Liq (5 nm)/Al (30 nm). With the detailed investigation of electroluminescence of the novel organic yellow-light-emitting device, the authors found that the doping concentration of Rhodamine B (RhB) had a very big influence on luminance and efficiency of the organic yellow-light-emitting device. When doping concentration of Rhodamine B (RhB) was 1.5 wt%, the organic yellow-light-emitting device was obtained with the maximum current efficiency of 1.526 cd x A(-1) and the maximum luminance of 1 309 cd x m(-2). It can be seen from the EL spectra of the devices that there existed energy transferring from Alq3 to RhB in the organic light-emitting layers. When the doping concentration of RhB increased, lambda(max) of EL spectra redshifted obviously. The phenomenon was attributed to the Stokes effect of quantum wells and self-polarization of RhB dye molecules.
New records of larval stages of the eel cod genus Muraenolepis Günther 1880 (Gadiformes: Muraenolepididae) from the western Antarctic Peninsula.

PubMed

Konstantinidis, P; Hilton, E J; Matarese, A C

2016-08-01

Three newly discovered larval specimens of the genus Muraenolepis collected from the waters of the western Antarctic Peninsula are described. Knowledge of their natural history is sparse and information about their early life history is based on only a few larval stages. Here, the available literature on larval eel cods is reviewed, and the specimens placed in context. © 2016 The Fisheries Society of the British Isles.
21 CFR 74.2711 - D&C Yellow No. 11.

Code of Federal Regulations, 2010 CFR

2010-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2711 D&C Yellow No. 11. (a) Identity and specifications. The color additive D&C Yellow No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2706 - FD&C Yellow No. 6.

Code of Federal Regulations, 2010 CFR

2010-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2706 FD&C Yellow No. 6. (a) Identity and specifications. The color additive FD&C Yellow No. 6 shall conform in identity and specifications to the... coloring cosmetics generally in amounts consistent with current good manufacturing practice. (c) Labeling...
21 CFR 74.2708 - D&C Yellow No. 8.

Code of Federal Regulations, 2010 CFR

2010-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2708 D&C Yellow No. 8. (a) Identity and specifications. The color additive D&C Yellow No. 8 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2707 - D&C Yellow No. 7.

Code of Federal Regulations, 2010 CFR

2010-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2707 D&C Yellow No. 7. (a) Identity and specifications. The color additive D&C Yellow No. 7 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2707 - D&C Yellow No. 7.

Code of Federal Regulations, 2013 CFR

2013-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2707 D&C Yellow No. 7. (a) Identity and specifications. The color additive D&C Yellow No. 7 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2707 - D&C Yellow No. 7.

Code of Federal Regulations, 2014 CFR

2014-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2707 D&C Yellow No. 7. (a) Identity and specifications. The color additive D&C Yellow No. 7 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2711 - D&C Yellow No. 11.

Code of Federal Regulations, 2013 CFR

2013-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2711 D&C Yellow No. 11. (a) Identity and specifications. The color additive D&C Yellow No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2706 - FD&C Yellow No. 6.

Code of Federal Regulations, 2012 CFR

2012-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2706 FD&C Yellow No. 6. (a) Identity and specifications. The color additive FD&C Yellow No. 6 shall conform in identity and specifications to the... coloring cosmetics generally in amounts consistent with current good manufacturing practice. (c) Labeling...
21 CFR 74.2711 - D&C Yellow No. 11.

Code of Federal Regulations, 2011 CFR

2011-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2711 D&C Yellow No. 11. (a) Identity and specifications. The color additive D&C Yellow No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2708 - D&C Yellow No. 8.

Code of Federal Regulations, 2011 CFR

2011-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2708 D&C Yellow No. 8. (a) Identity and specifications. The color additive D&C Yellow No. 8 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2707 - D&C Yellow No. 7.

Code of Federal Regulations, 2012 CFR

2012-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2707 D&C Yellow No. 7. (a) Identity and specifications. The color additive D&C Yellow No. 7 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2708 - D&C Yellow No. 8.

Code of Federal Regulations, 2012 CFR

2012-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2708 D&C Yellow No. 8. (a) Identity and specifications. The color additive D&C Yellow No. 8 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2708 - D&C Yellow No. 8.

Code of Federal Regulations, 2013 CFR

2013-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2708 D&C Yellow No. 8. (a) Identity and specifications. The color additive D&C Yellow No. 8 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2706 - FD&C Yellow No. 6.

Code of Federal Regulations, 2013 CFR

2013-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2706 FD&C Yellow No. 6. (a) Identity and specifications. The color additive FD&C Yellow No. 6 shall conform in identity and specifications to the... coloring cosmetics generally in amounts consistent with current good manufacturing practice. (c) Labeling...
21 CFR 74.2711 - D&C Yellow No. 11.

Code of Federal Regulations, 2012 CFR

2012-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2711 D&C Yellow No. 11. (a) Identity and specifications. The color additive D&C Yellow No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...

21 CFR 74.2706 - FD&C Yellow No. 6.

Code of Federal Regulations, 2014 CFR

2014-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2706 FD&C Yellow No. 6. (a) Identity and specifications. The color additive FD&C Yellow No. 6 shall conform in identity and specifications to the... coloring cosmetics generally in amounts consistent with current good manufacturing practice. (c) Labeling...
21 CFR 74.2708 - D&C Yellow No. 8.

Code of Federal Regulations, 2014 CFR

2014-04-01

... ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2708 D&C Yellow No. 8. (a) Identity and specifications. The color additive D&C Yellow No. 8 shall conform in identity and specifications to the requirements... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
21 CFR 74.2711 - D&C Yellow No. 11.

Code of Federal Regulations, 2014 CFR

2014-04-01

... COLOR ADDITIVES SUBJECT TO CERTIFICATION Cosmetics § 74.2711 D&C Yellow No. 11. (a) Identity and specifications. The color additive D&C Yellow No. 11 shall conform in identity and specifications to the... coloring externally applied cosmetics in amounts consistent with good manufacturing practice. (c) Labeling...
Quantification of bupivacaine hydrochloride and isoflupredone acetate residues in porcine muscle, beef, milk, egg, shrimp, flatfish, and eel using a simplified extraction method coupled with liquid chromatography-triple quadrupole tandem mass spectrometry.

PubMed

Cho, Sang-Hyun; Park, Jin-A; Zheng, Weijia; Abd El-Aty, A M; Kim, Seong-Kwan; Choi, Jeong-Min; Yi, Hee; Cho, Soo-Min; Afifi, Nehal A; Shim, Jae-Han; Chang, Byung-Joon; Kim, Jin-Suk; Shin, Ho-Chul

2017-10-15

In this study, a simple analytical approach has been developed and validated for the determination of bupivacaine hydrochloride and isoflupredone acetate residues in porcine muscle, beef, milk, egg, shrimp, flatfish, and eel using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A 0.1% solution of acetic acid in acetonitrile combined with n-hexane was used for deproteinization and defatting of all tested matrices and the target drugs were well separated on a Waters Xbridge™ C18 analytical column using a mobile phase consisting of 0.1% acetic acid (A) and 0.1% solution of acetic acid in methanol (B). The linearity estimated from six-point matrix-matched calibrations was good, with coefficients of determination ≥0.9873. The limits of quantification (LOQs) for bupivacaine hydrochloride and isoflupredone acetate were 1 and 2ngg -1 , respectively. Recovery percentages in the ranges of 72.51-112.39% (bupivacaine hydrochloride) and 72.58-114.56% (isoflupredone acetate) were obtained from three different fortification concentrations with relative standard deviations (RSDs) of <15.14%. All samples for the experimental work and method application were collected from the local markets in Seoul, Republic of Korea, and none of them tested positive for the target drugs. In conclusion, a simple method using a 0.1% solution of acetic acid in acetonitrile and n-hexane followed by LC-MS/MS could effectively extract bupivacaine hydrochloride and isoflupredone acetate from porcine muscle, beef, milk, egg, shrimp, flatfish, and eel samples. Copyright © 2017 Elsevier B.V. All rights reserved.
Hierarchical mean-field approach to the J1-J2 Heisenberg model on a square lattice

NASA Astrophysics Data System (ADS)

Isaev, Leonid; Ortiz, Gerardo; Dukelsky, Jorge

2009-03-01

We study the quantum phase diagram and excitation spectrum of the frustrated J1-J2 spin-1/2 Heisenberg Hamiltonian. A hierarchical mean-field approach, at the heart of which lies the idea of identifying relevant degrees of freedom, is developed. Thus, by performing educated, manifestly symmetry preserving mean-field approximations, we unveil fundamental properties of the system. We then compare various coverings of the square lattice with plaquettes, dimers and other degrees of freedom, and show that only the symmetric plaquette covering, which reproduces the original Bravais lattice, leads to the known phase diagram. The intermediate quantum paramagnetic phase is shown to be a (singlet) plaquette crystal, connected with the neighbouring N'eel phase by a continuous phase transition. We also introduce fluctuations around the hierarchical mean-field solutions, and demonstrate that in the paramagnetic phase the ground and first excited states are separated by a finite gap, which closes in the N'eel and columnar phases. Our results suggest that the quantum phase transition between N'eel and paramagnetic phases can be properly described within the Ginzburg-Landau-Wilson paradigm.
PAHs behavior in surface water and groundwater of the Yellow River estuary: Evidence from isotopes and hydrochemistry.

PubMed

Li, Jing; Li, Fadong; Liu, Qiang

2017-07-01

Large-scale irrigation projects have impacted the regional surface-groundwater interactions in the North China Plain (NCP). Given this concern, the aim of this study is to evaluate levels of PAH pollution, identify the sources of the PAHs, analyze the influence of surface-groundwater interactions on PAH distribution, and propose urgent management strategies for PAHs in China's agricultural areas. PAH concentrations, hydrochemical indicators and stable isotopic compositions (δ 18 O and δ 2 H) were determined for surface water (SW) and groundwater (GW) samples. PAHs concentrations in surface water and groundwater varied from 11.84 to 393.12 ng/L and 8.51-402.84 ng/L, respectively, indicating mild pollution. The seasonal variations showed the following trend: PAHs in surface water at the low-water phase > PAHs in groundwater at the low-water phase > PAHs in surface water at the high-water phase > PAHs in groundwater at the high-water phase. Hydrochemical and δ 18 O value of most groundwater samples distributed between the Yellow River and seawater. The mean value of mixture ratio of the Yellow River water recharge to the groundwater was 65%, few anomalous sites can reach to 90%. Surface-groundwater interactions influence the spatial distribution of PAHs in the study area. In light of the ongoing serious pollution, management practices for source control, improved control technologies, and the construction of a monitoring network to warn of increased risk are urgently needed. Copyright © 2017 Elsevier Ltd. All rights reserved.
Application of isostatic gravity anomaly in the Yellow Sea area

NASA Astrophysics Data System (ADS)

Hao, Z.; Qin, J.; Huang, W.; Wu, X.

2017-12-01

In order to study the deep crustal structure of the Yellow Sea area, we used the Airy-Heiskanen model to calculate the isostatic gravity anomaly of this area. Based on the Bouguer gravity anomaly and water depth data of this area, we chose the calculating parameters as standard crustal thickness 30 km, crust-mantle density difference 0.6g/cm3and grid spacing 0.1°×0.1°. This study reveals that there are six faults and four isostatic negative anomalies in the study area. The isostatic anomalies in much of Yellow Sea areas give priority to those with positive anomalies. The isostatic anomalies in North Yellow Sea are higher than South Yellow Sea with Jiashan-Xiangshui fault as the boundary. In the north of the study area, isostatic anomalies are characterized by large areas of positive anomaly. The change is relatively slow, and the trends give priority to the trend NE or NEE. In the middle of the north Yellow Sea basin, there is a local negative anomaly, arranged as a string of beads in NE to discontinuous distribution. Negative anomaly range is small, basically corresponds to the region's former Cenozoic sedimentary basin position. To the south of Jiashan-Xiangshui fault and west of Yellow Sea eastern margin fault, including most of the south Yellow Sea and Jiangsu province, the isostatic anomalies are lower. And the positive and negative anomalies are alternative distribution, and negative anomaly trap in extensive development. The trends give priority to NE, NEE, both to the NW. On the basis of the characteristics of isostatic gravity anomalies, it is concluded that the Yellow Sea belongs to continental crustal isostatic area whose isostatic anomalies is smooth and slow. ReferencesHeiskanen, W. A., F. A. V. Meinesz, and S. A. Korff (1958), The Earth and Its Gravity Field, McGraw-Hill, New York. Meng, X. J., X. H. Zhang, and J. Y. Yang (2014), Geophysical survey in eastern China seas and the characteristics of gravity and magnetic fields, Marine Geoglogy
Vector competence of Australian mosquitoes for yellow fever virus.

PubMed

van den Hurk, Andrew F; McElroy, Kate; Pyke, Alyssa T; McGee, Charles E; Hall-Mendelin, Sonja; Day, Andrew; Ryan, Peter A; Ritchie, Scott A; Vanlandingham, Dana L; Higgs, Stephen

2011-09-01

The vector competence of Australian mosquitoes for yellow fever virus (YFV) was evaluated. Infection and transmission rates in Cairns and Townsville populations of Aedes aegypti and a Brisbane strain of Ae. notoscriptus were not significantly different from a well-characterized YFV-susceptible strain of Ae. aegypti. After exposure to 10⁷·² tissue culture infectious dose (TCID₅₀)/mL of an African strain of YFV, > 70% of Ae. aegypti and Ae. notoscriptus became infected, and > 50% transmitted the virus. When exposed to 10⁶·⁷) TCID₅₀/mL of a South American strain of YFV, the highest infection (64%) and transmission (56%) rates were observed in Ae. notoscriptus. The infection and transmission rates in the Cairns Ae. aegypti were both 24%, and they were 36% and 28%, respectively, for the Townsville population. Because competent vectors are present, the limited number of travelers from endemic areas and strict vaccination requirements will influence whether YFV transmission occurs in Australia.
IRAS 18357-0604 - an analogue of the galactic yellow hypergiant IRC +10420?

NASA Astrophysics Data System (ADS)

Clark, J. S.; Negueruela, I.; González-Fernández, C.

2014-01-01

Context. Yellow hypergiants represent a short-lived evolutionary episode experienced by massive stars as they transit to and from a red supergiant phase. As such, their properties provide a critical test of stellar evolutionary theory, while recent observations unexpectedly suggest that a subset may explode as Type II supernovae. Aims: The galactic yellow hypergiant IRC +10420 is a cornerstone system for understanding this phase since it is the strongest post-RSG candidate known, has demonstrated real-time evolution across the Hertzsprung-Russell diagram and been subject to extensive mass loss. In this paper we report on the discovery of a twin of IRC +10420 - IRAS 18357-0604. Methods: Optical and near-IR spectroscopy are used to investigate the physical properties of IRAS 18357-0604 and also provide an estimate of its systemic velocity, while near- to mid-IR photometry probes the nature of its circumstellar environment. Results: These observations reveal pronounced spectral similarities between IRAS 18357-0604 and IRC +10420, suggesting comparable temperatures and wind geometries. IR photometric data reveals a similarly dusty circumstellar environment, although historical mass loss appears to have been heavier in IRC +10420. The systemic velocity implies a distance compatible with the red supergiant-dominated complex at the base of the Scutum Crux arm; the resultant luminosity determination is consistent with a physical association but suggests a lower initial mass than inferred for IRC +10420 (≲20 M⊙ versus ~40 M⊙). Evolutionary predictions for the physical properties of supernova progenitors derived from ~18-20 M⊙ stars - or ~12-15 M⊙ stars that have experienced enhanced mass loss as red supergiants - compare favourably with those of IRAS 18357-0604, which in turn appears to be similar to the the progenitor of SN2011dh; it may therefore provide an important insight into the nature of the apparently H-depleted yellow hypergiant progenitors of some Type
Complete genome sequence and phylogenetic analyses of an aquabirnavirus isolated from a diseased marbled eel culture in Taiwan.

PubMed

Wen, Chiu-Ming

2017-08-01

An aquabirnavirus was isolated from diseased marbled eels (Anguilla marmorata; MEIPNV1310) with gill haemorrhages and associated mortality. Its genome segment sequences were obtained through next-generation sequencing and compared with published aquabirnavirus sequences. The results indicated that the genome sequence of MEIPNV1310 contains segment A (3099 nucleotides) and segment B (2789 nucleotides). Phylogenetic analysis showed that MEIPNV1310 is closely related to the infectious pancreatic necrosis Ab strain within genogroup II. This genome sequence is beneficial for studying the geographic distribution and evolution of aquabirnaviruses.
Regional variability of slope stability: Application to the Eel margin, California

USGS Publications Warehouse

Lee, H.; Locat, J.; Dartnell, P.; Israel, K.; Florence, Wong

1999-01-01

Relative values of downslope driving forces and sediment resisting forces determine the locations of submarine slope failures. Both of these vary regionally, and their impact can be addressed when the data are organized in a Geographic Information System (GIS). The study area on the continental margin near the Eel River provides an excellent opportunity to apply GIS spatial analysis techniques for evaluation of slope stability. In this area, swath bathymetric mapping shows seafloor morphology and distribution of slope steepness in fine detail, and sediment analysis of over 70 box cores delineates the variability of sediment density near the seafloor surface. Based on the results of ten geotechnical studies of submarine study areas, we developed an algorithm that relates surface sediment density to the shear strength appropriate to the type of cyclic loading produced by an earthquake. Strength and stress normalization procedures provide results that are conceptually independent of subbottom depth. Results at depth are rigorously applicable if sediment lithology does not vary significantly and consolidation state can be estimated. Otherwise, the method applies only to shallow-seated slope failure. Regional density, slope, and level of anticipated seismic shaking information were combined in a GIS framework to yield a map that illustrates the relative stability of slopes in the face of seismically induced failure. When a measure of predicted relative slope stability is draped on an oblique view of swath bathymetry, a variation in this slope stability is observed on an otherwise smooth slope along the mid-slope region north of a plunging anticline. The section of slope containing diffuse, pockmarked gullies has a lower measure of stability than a separate section containing gullies that have sharper boundaries and somewhat steeper sides. Such an association suggests that our slope-stability analysis relates to the stability of the gully sides. The remainder of the
'Yellow lens' eyes of a stomiatoid deep-sea fish, Malacosteus niger.

PubMed

Somiya, H

1982-07-22

Bright yellow lenses were found in the eyes of the stomiatoid deep-sea fish, Malacosteus niger Ayres. The optical properties of the yellow lens and the retinal specializations in the eyes were examined. Absorption spectra of the yellow lens revealed two peaks at wavelengths 425 and 460 nm. The photoreceptors were all rods and were arranged in two superimposed layers. An astaxanthin-type retinal tapetum was observed in the pigment epithelium. Some chemical evidence is presented showing that the tapetal material is an astaxanthin ester. The ecological significance of the yellow lens is discussed in connection with that of Malacosteus' orbital light organ which has a reddish filter.
Water security evaluation in Yellow River basin

NASA Astrophysics Data System (ADS)

Jiang, Guiqin; He, Liyuan; Jing, Juan

2018-03-01

Water security is an important basis for making water security protection strategy, which concerns regional economic and social sustainable development. In this paper, watershed water security evaluation index system including 3 levels of 5 criterion layers (water resources security, water ecological security and water environment security, water disasters prevention and control security and social economic security) and 24 indicators were constructed. The entropy weight method was used to determine the weights of the indexes in the system. The water security index of 2000, 2005, 2010 and 2015 in Yellow River basin were calculated by linear weighting method based on the relative data. Results show that the water security conditions continue to improve in Yellow River basin but still in a basic security state. There is still a long way to enhance the water security in Yellow River basin, especially the water prevention and control security, the water ecological security and water environment security need to be promoted vigorously.
Elongation of the body in eels.

PubMed

Mehta, Rita S; Ward, Andrea B; Alfaro, Michael E; Wainwright, Peter C

2010-12-01

The shape of the body affects how organisms move, where they live, and how they feed. One body plan that has long engaged the interest of both evolutionary biologists and functional morphologists is axial elongation. There is a growing interest in the correlates and evolution of elongation within different terrestrial and aquatic vertebrate clades. At first glance, Anguilliformes may appear to exhibit a single cylindrical form but there is considerable diversity underlying this seemingly simplified body plan. Here, we explore evolution of the axial skeleton in 54 anguilliform taxa and some close relatives. We describe the diversity of axial elongation as well as investigate how characters such as head length, branchial-arch length, and shape of the pectoral fins correlate with vertebral number to possibly facilitate changes in absolute diameter of the body. Overall, we find that precaudal vertebral numbers and caudal vertebral numbers are evolving independently across elopomorph fishes. We also find that precaudal and caudal vertebral aspect ratios are evolving together across elopomorph fishes. When focusing within Anguilliformes we find striking diversity in the mechanisms of elongation of the body, including almost every trend for axial elongation known within actinopterygian fishes. The three major clades of eels we examined have slightly different mechanisms of elongation. We also find a suite of morphological characters associated with elongation in anguilliform fishes that appears to coincide with a more fossorial lifestyle such as high elongation ratios, a more posteriorly extended-branchial region, and a reduction in the size of the pectoral fins. Lastly, we point out that a diverse range of derived behaviors such as head- and tail-first burrowing, rotational feeding, and knotting around prey are only found in long cylindrical vertebrates.
Traveling Abroad: Latest Yellow Fever Vaccine Update | Poster

Cancer.gov

Earlier this month, the U.S. Centers for Disease Control and Prevention (CDC) released its list of clinics that are administering the yellow fever vaccine Stamaril, which has been made available to address the total depletion of the United States’ primary yellow fever vaccine, YF-VAX. These clinics will provide the vaccine to individuals preparing for international travel, including NCI at Frederick staff and scientists.
76 FR 60431 - Endangered and Threatened Wildlife and Plants; 90-Day Finding on a Petition To List the American...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-29

... SST that affect primary productivity; (b) Quantitative research on the food of eel larvae and the... 4967, p. 4996). The Service suggested that ``future research should focus on: The effects of... ``While it is possible that some eels spend their entire life cycle in salt water, oceanic research...
Cucurbit chlorotic yellows virus

USDA-ARS?s Scientific Manuscript database

Cucurbit chlorotic yellows virus (CCYV) emerged as a threat to cucurbit production in Japan during the early 2000s and has since spread to China and Taiwan, as well as to the Middle East, and parts of Africa. CCYV (genus Crinivirus, family Closteroviridae) causes chlorotic mottle symptoms, intervein...
Protein transduction domain of transactivating transcriptional activator fused to outer membrane protein K of Vibrio parahaemolyticus to vaccinate marbled eels (Anguilla marmorata) confers protection against mortality caused by V. parahaemolyticus.

PubMed

Wang, Hang; Yang, Wei; Shen, Guoying; Zhang, Jianting; Lv, Wei; Ji, Binfeng; Meng, Chun

2015-07-01

Although immersion and oral vaccination are the most practical methods for fish farmers, their applications are very limited due to low immune stimulation effect. We used the protein transduction domain (PTD) of transactivating transcriptional factor (TAT) derived from HIV TAT protein to increase the delivery efficiency of aquatic protein vaccines. Vibrio parahaemolyticus outer membrane protein K (ompK), a reported vaccine candidate for the prevention of V. parahaemolyticus infection, was fused with TAT-PTD expressed in Escherichia coli. We found that PTD-ompK fusion protein effectively penetrated into marbled eel bodies. Analysis of ompK antibody titres demonstrated that immersion vaccination with PTD-ompK was superior to ompK alone and induced robust immune stimulation in marbled eels. Both active and passive protection analyses against immersive challenge with V. parahaemolyticus strains demonstrated that marbled eels immunized with PTD-ompK survived significantly longer than those immunized with ompK alone. Our results indicated that TAT-PTD could be served as is an efficient delivery system for aquatic immersion vaccinations against various infectious diseases commonly seen in aquatic farm industry. © 2015 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
Habitat Suitability Index Models: Yellow perch

USGS Publications Warehouse

Krieger, Douglas A.; Terrell, James W.; Nelson, Patrick C.

1983-01-01

A review and synthesis of existing information were used to develop riverine and lacustrine habitat models for yellow perch (Perca flavescens). The models are scaled to produce an index of habitat suitability between 0 (unsuitable habitat) to 1 (optimally suitable habitat) for riverine, lacustrine, and palustrine habitat in the 48 contiguous United States. Habitat Suitability Indexes (HSI's) are designed for use with the Habitat Evaluation Procedures developed by the U.S. Fish and Wildlife Service. Also included are discussions of Suitability Index (SI) curves as used in the Instream Flow Incremental Methodology (IFIM) and SI curves available for an IFIM analysis of yellow perch habitat.
Safety issues from a Phase 3 clinical trial of a live-attenuated chimeric yellow fever tetravalent dengue vaccine.

PubMed

Halstead, Scott B

2018-02-26

A tetravalent live-attenuated 3-dose vaccine composed of chimeras of yellow fever 17D and the four dengue viruses (CYD, also called Dengvaxia) completed phase 3 clinical testing in over 35,000 children leading to a recommendation that vaccine be administered to >/ = 9 year-olds residing in highly dengue- endemic countries. When clinical trial results were assessed 2 years after the first dose, vaccine efficacy among seropositives was high, but among seronegatives efficacy was marginal. Breakthrough dengue hospitalizations of vaccinated children occurred continuously over a period of 4-5 years post 3rd dose in an age distribution suggesting these children had been vaccinated when seronegative. This surmise was validated recently when the manufacturer reported that dengue NS1 IgG antibodies were absent in sera from hospitalized vaccinated children, an observation consistent with their having received Dengvaxia when seronegative. Based upon published efficacy data and in compliance with initial published recommendations by the manufacturer and WHO the Philippine government undertook to vaccinate 800,000-plus 9 year-olds starting in April 2016. Eighteen months later, dengue hospitalizations and a deaths were reported among vaccinated children. The benefits of administering Dengvaxia predicted by the manufacturer, WHO and others derive from scoring dengue hospitalizations of vaccinated children as vaccine failures rather than as vaccine enhanced dengue disease. Recommended regimens for administration of Dengvaxia should have been structured to warn of and avoid serious adverse events.

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