Sample records for yield stress increases

  1. Coordinated regulation of photosynthesis in rice increases yield and tolerance to environmental stress

    PubMed Central

    Ambavaram, Madana M. R.; Basu, Supratim; Krishnan, Arjun; Ramegowda, Venkategowda; Batlang, Utlwang; Rahman, Lutfor; Baisakh, Niranjan; Pereira, Andy

    2014-01-01

    Plants capture solar energy and atmospheric carbon dioxide (CO2) through photosynthesis, which is the primary component of crop yield, and needs to be increased considerably to meet the growing global demand for food. Environmental stresses, which are increasing with climate change, adversely affect photosynthetic carbon metabolism (PCM) and limit yield of cereals such as rice (Oryza sativa) that feeds half the world. To study the regulation of photosynthesis, we developed a rice gene regulatory network and identified a transcription factor HYR (HIGHER YIELD RICE) associated with PCM, which on expression in rice enhances photosynthesis under multiple environmental conditions, determining a morpho-physiological programme leading to higher grain yield under normal, drought and high-temperature stress conditions. We show HYR is a master regulator, directly activating photosynthesis genes, cascades of transcription factors and other downstream genes involved in PCM and yield stability under drought and high-temperature environmental stress conditions. PMID:25358745

  2. Morphological plasticity of root growth under mild water stress increases water use efficiency without reducing yield in maize

    NASA Astrophysics Data System (ADS)

    Cai, Qian; Zhang, Yulong; Sun, Zhanxiang; Zheng, Jiaming; Bai, Wei; Zhang, Yue; Liu, Yang; Feng, Liangshan; Feng, Chen; Zhang, Zhe; Yang, Ning; Evers, Jochem B.; Zhang, Lizhen

    2017-08-01

    A large yield gap exists in rain-fed maize (Zea mays L.) production in semi-arid regions, mainly caused by frequent droughts halfway through the crop-growing period due to uneven distribution of rainfall. It is questionable whether irrigation systems are economically required in such a region since the total amount of rainfall does generally meet crop requirements. This study aimed to quantitatively determine the effects of water stress from jointing to grain filling on root and shoot growth and the consequences for maize grain yield, above- and below-ground dry matter, water uptake (WU) and water use efficiency (WUE). Pot experiments were conducted in 2014 and 2015 with a mobile rain shelter to achieve conditions of no, mild or severe water stress. Maize yield was not affected by mild water stress over 2 years, while severe stress reduced yield by 56 %. Both water stress levels decreased root biomass slightly but shoot biomass substantially. Mild water stress decreased root length but increased root diameter, resulting in no effect on root surface area. Due to the morphological plasticity in root growth and the increase in root / shoot ratio, WU under water stress was decreased, and overall WUE for both above-ground dry matter and grain yield increased. Our results demonstrate that an irrigation system might be not economically and ecologically necessary because the frequently occurring mild water stress did not reduce crop yield much. The study helps us to understand crop responses to water stress during a critical water-sensitive period (middle of the crop-growing season) and to mitigate drought risk in dry-land agriculture.

  3. Increasing influence of heat stress on French maize yields from the 1960s to the 2030s

    PubMed Central

    Hawkins, Ed; Fricker, Thomas E; Challinor, Andrew J; Ferro, Christopher A T; Kit Ho, Chun; Osborne, Tom M

    2013-01-01

    Improved crop yield forecasts could enable more effective adaptation to climate variability and change. Here, we explore how to combine historical observations of crop yields and weather with climate model simulations to produce crop yield projections for decision relevant timescales. Firstly, the effects on historical crop yields of improved technology, precipitation and daily maximum temperatures are modelled empirically, accounting for a nonlinear technology trend and interactions between temperature and precipitation, and applied specifically for a case study of maize in France. The relative importance of precipitation variability for maize yields in France has decreased significantly since the 1960s, likely due to increased irrigation. In addition, heat stress is found to be as important for yield as precipitation since around 2000. A significant reduction in maize yield is found for each day with a maximum temperature above 32 °C, in broad agreement with previous estimates. The recent increase in such hot days has likely contributed to the observed yield stagnation. Furthermore, a general method for producing near-term crop yield projections, based on climate model simulations, is developed and utilized. We use projections of future daily maximum temperatures to assess the likely change in yields due to variations in climate. Importantly, we calibrate the climate model projections using observed data to ensure both reliable temperature mean and daily variability characteristics, and demonstrate that these methods work using retrospective predictions. We conclude that, to offset the projected increased daily maximum temperatures over France, improved technology will need to increase base level yields by 12% to be confident about maintaining current levels of yield for the period 2016–2035; the current rate of yield technology increase is not sufficient to meet this target. PMID:23504849

  4. Breeding approaches and genomics technologies to increase crop yield under low-temperature stress.

    PubMed

    Jha, Uday Chand; Bohra, Abhishek; Jha, Rintu

    2017-01-01

    Improved knowledge about plant cold stress tolerance offered by modern omics technologies will greatly inform future crop improvement strategies that aim to breed cultivars yielding substantially high under low-temperature conditions. Alarmingly rising temperature extremities present a substantial impediment to the projected target of 70% more food production by 2050. Low-temperature (LT) stress severely constrains crop production worldwide, thereby demanding an urgent yet sustainable solution. Considerable research progress has been achieved on this front. Here, we review the crucial cellular and metabolic alterations in plants that follow LT stress along with the signal transduction and the regulatory network describing the plant cold tolerance. The significance of plant genetic resources to expand the genetic base of breeding programmes with regard to cold tolerance is highlighted. Also, the genetic architecture of cold tolerance trait as elucidated by conventional QTL mapping and genome-wide association mapping is described. Further, global expression profiling techniques including RNA-Seq along with diverse omics platforms are briefly discussed to better understand the underlying mechanism and prioritize the candidate gene (s) for downstream applications. These latest additions to breeders' toolbox hold immense potential to support plant breeding schemes that seek development of LT-tolerant cultivars. High-yielding cultivars endowed with greater cold tolerance are urgently required to sustain the crop yield under conditions severely challenged by low-temperature.

  5. Local yield stress statistics in model amorphous solids

    NASA Astrophysics Data System (ADS)

    Barbot, Armand; Lerbinger, Matthias; Hernandez-Garcia, Anier; García-García, Reinaldo; Falk, Michael L.; Vandembroucq, Damien; Patinet, Sylvain

    2018-03-01

    We develop and extend a method presented by Patinet, Vandembroucq, and Falk [Phys. Rev. Lett. 117, 045501 (2016), 10.1103/PhysRevLett.117.045501] to compute the local yield stresses at the atomic scale in model two-dimensional Lennard-Jones glasses produced via differing quench protocols. This technique allows us to sample the plastic rearrangements in a nonperturbative manner for different loading directions on a well-controlled length scale. Plastic activity upon shearing correlates strongly with the locations of low yield stresses in the quenched states. This correlation is higher in more structurally relaxed systems. The distribution of local yield stresses is also shown to strongly depend on the quench protocol: the more relaxed the glass, the higher the local plastic thresholds. Analysis of the magnitude of local plastic relaxations reveals that stress drops follow exponential distributions, justifying the hypothesis of an average characteristic amplitude often conjectured in mesoscopic or continuum models. The amplitude of the local plastic rearrangements increases on average with the yield stress, regardless of the system preparation. The local yield stress varies with the shear orientation tested and strongly correlates with the plastic rearrangement locations when the system is sheared correspondingly. It is thus argued that plastic rearrangements are the consequence of shear transformation zones encoded in the glass structure that possess weak slip planes along different orientations. Finally, we justify the length scale employed in this work and extract the yield threshold statistics as a function of the size of the probing zones. This method makes it possible to derive physically grounded models of plasticity for amorphous materials by directly revealing the relevant details of the shear transformation zones that mediate this process.

  6. Overexpression of the brassinosteroid biosynthetic gene DWF4 in Brassica napus simultaneously increases seed yield and stress tolerance

    PubMed Central

    Sahni, Sangita; Prasad, Bishun D.; Liu, Qing; Grbic, Vojislava; Sharpe, Andrew; Singh, Surinder P.; Krishna, Priti

    2016-01-01

    As a resource allocation strategy, plant growth and defense responses are generally mutually antagonistic. Brassinosteroid (BR) regulates many aspects of plant development and stress responses, however, genetic evidence of its integrated effects on plant growth and stress tolerance is lacking. We overexpressed the Arabidopsis BR biosynthetic gene AtDWF4 in the oilseed plant Brassica napus and scored growth and stress response phenotypes. The transgenic B. napus plants, in comparison to wild type, displayed increased seed yield leading to increased overall oil content per plant, higher root biomass and root length, significantly better tolerance to dehydration and heat stress, and enhanced resistance to necrotrophic fungal pathogens Leptosphaeria maculans and Sclerotinia sclerotiorum. Transcriptome analysis supported the integrated effects of BR on growth and stress responses; in addition to BR responses associated with growth, a predominant plant defense signature, likely mediated by BES1/BZR1, was evident in the transgenic plants. These results establish that BR can interactively and simultaneously enhance abiotic and biotic stress tolerance and plant productivity. The ability to confer pleiotropic beneficial effects that are associated with different agronomic traits suggests that BR–related genes may be important targets for simultaneously increasing plant productivity and performance under stress conditions. PMID:27324083

  7. Increasing Crop Yields in Water Stressed Countries by Combining Operations of Freshwater Reservoir and Wastewater Reclamation Plant

    NASA Astrophysics Data System (ADS)

    Bhushan, R.; Ng, T. L.

    2015-12-01

    Freshwater resources around the world are increasing in scarcity due to population growth, industrialization and climate change. This is a serious concern for water stressed countries, including those in Asia and North Africa where future food production is expected to be negatively affected by this. To address this problem, we investigate the potential of combining freshwater reservoir and wastewater reclamation operations. Reservoir water is the cheaper source of irrigation, but is often limited and climate sensitive. Treated wastewater is a more reliable alternative for irrigation, but often requires extensive further treatment which can be expensive. We propose combining the operations of a reservoir and a wastewater reclamation plant (WWRP) to augment the supply from the reservoir with reclaimed water for increasing crop yields in water stressed regions. The joint system of reservoir and WWRP is modeled as a multi-objective optimization problem with the double objective of maximizing the crop yield and minimizing total cost, subject to constraints on reservoir storage, spill and release, and capacity of the WWRP. We use the crop growth model Aquacrop, supported by The Food and Agriculture Organization of the United Nations (FAO), to model crop growth in response to water use. Aquacrop considers the effects of water deficit on crop growth stages, and from there estimates crop yield. We generate results comparing total crop yield under irrigation with water from just the reservoir (which is limited and often interrupted), and yield with water from the joint system (which has the potential of higher supply and greater reliability). We will present results for locations in India and Africa to evaluate the potential of the joint operations for improving food security in those areas for different budgets.

  8. Scaling and Thermal Evolution of Internally Heated Planets: Yield Stress and Thermal History.

    NASA Astrophysics Data System (ADS)

    Weller, M. B.; Lenardic, A.; Moore, W. B.

    2014-12-01

    Using coupled 3D mantle convection and planetary tectonics models of bi-stable systems, we show how system behaviors for mobile-lid and stagnant-lid states scale as functions of internal heating rates (Q) and basal Ra (Rab). With parameter ranges for temperature- and depth-dependant viscosities: 1e4 - 3e4, Rab: 1e5- 3e5, Q: 0 - 100, and yield stress: 1e4 - 2e5, it can be shown the internal temperatures, velocities, heat fluxes, and system behaviors for mobile-lid and stagnant-lid states diverge, for equivalent parameter values, as a function of increasing Q. For the mobile-lid regime, yielding behavior in the upper boundary layer strongly influences the dynamics of the system. Internal temperatures, and consequently temperature-dependant viscosities, vary strongly as a function of yield stress for a given Q. The temperature distribution across the upper and lower mantles are sub-adiabatic for low to moderate yield stress, and adiabatic to super-adiabatic for high yield stresses. Across the parameter range considered, and for fixed yield stress, the Nu across the basal boundary (Nub) is positive and only weakly dependant on Q (varies by ~ 9%). Nub varies strongly as a function of yield stress (maximum variation of ~84%). Both mobile-lid velocities and lid-thicknesses are yield stress dependant for a given Q and Ra. In contrast to mobile-lids, the stagnant-lid regime is governed by the relative inefficiency of heat transport through the surface boundary layer. Internal temperatures are yield stress independent, and are on average 30% greater. Nub has a strong dependence on heating rates and surface boundary layer thicknesses. Within the parameter space considered, the maximum stagnant-lid Nub corresponds to the minimum mobile-lid Nub (for high yield stress), and decreases with increasing Q. For high Q, super-heated stagnant-lids may develop, with Nub< 0, and changes in trends for system behaviors. Planets with high levels of internal heating and/or high yield

  9. Characterizing drought stress and trait influence on maize yield under current and future conditions.

    PubMed

    Harrison, Matthew T; Tardieu, François; Dong, Zhanshan; Messina, Carlos D; Hammer, Graeme L

    2014-03-01

    Global climate change is predicted to increase temperatures, alter geographical patterns of rainfall and increase the frequency of extreme climatic events. Such changes are likely to alter the timing and magnitude of drought stresses experienced by crops. This study used new developments in the classification of crop water stress to first characterize the typology and frequency of drought-stress patterns experienced by European maize crops and their associated distributions of grain yield, and second determine the influence of the breeding traits anthesis-silking synchrony, maturity and kernel number on yield in different drought-stress scenarios, under current and future climates. Under historical conditions, a low-stress scenario occurred most frequently (ca. 40%), and three other stress types exposing crops to late-season stresses each occurred in ca. 20% of cases. A key revelation shown was that the four patterns will also be the most dominant stress patterns under 2050 conditions. Future frequencies of low drought stress were reduced by ca. 15%, and those of severe water deficit during grain filling increased from 18% to 25%. Despite this, effects of elevated CO2 on crop growth moderated detrimental effects of climate change on yield. Increasing anthesis-silking synchrony had the greatest effect on yield in low drought-stress seasonal patterns, whereas earlier maturity had the greatest effect in crops exposed to severe early-terminal drought stress. Segregating drought-stress patterns into key groups allowed greater insight into the effects of trait perturbation on crop yield under different weather conditions. We demonstrate that for crops exposed to the same drought-stress pattern, trait perturbation under current climates will have a similar impact on yield as that expected in future, even though the frequencies of severe drought stress will increase in future. These results have important ramifications for breeding of maize and have implications for

  10. Post-heading heat stress and yield impact in winter wheat of China.

    PubMed

    Liu, Bing; Liu, Leilei; Tian, Liying; Cao, Weixing; Zhu, Yan; Asseng, Senthold

    2014-02-01

    Wheat is sensitive to high temperatures, but the spatial and temporal variability of high temperature and its impact on yield are often not known. An analysis of historical climate and yield data was undertaken to characterize the spatial and temporal variability of heat stress between heading and maturity and its impact on wheat grain yield in China. Several heat stress indices were developed to quantify heat intensity, frequency, and duration between heading and maturity based on measured maximum temperature records of the last 50 years from 166 stations in the main wheat-growing region of China. Surprisingly, heat stress between heading and maturity was more severe in the generally cooler northern wheat-growing regions than the generally warmer southern regions of China, because of the delayed time of heading with low temperatures during the earlier growing season and the exposure of the post-heading phase into the warmer part of the year. Heat stress between heading and maturity has increased in the last decades in most of the main winter wheat production areas of China, but the rate was higher in the south than in the north. The correlation between measured grain yields and post-heading heat stress and average temperature were statistically significant in the entire wheat-producing region, and explained about 29% of the observed spatial and temporal yield variability. A heat stress index considering the duration and intensity of heat between heading and maturity was required to describe the correlation of heat stress and yield variability. Because heat stress is a major cause of yield loss and the number of heat events is projected to increase in the future, quantifying the future impact of heat stress on wheat production and developing appropriate adaptation and mitigation strategies are critical for developing food security policies in China and elsewhere. © 2013 John Wiley & Sons Ltd.

  11. Genetic effects of heat stress on milk yield of Thai Holstein crossbreds.

    PubMed

    Boonkum, W; Misztal, I; Duangjinda, M; Pattarajinda, V; Tumwasorn, S; Sanpote, J

    2011-01-01

    The threshold for heat stress on milk yield of Holstein crossbreds under climatic conditions in Thailand was investigated, and genetic effects of heat stress on milk yield were estimated. Data included 400,738 test-day milk yield records for the first 3 parities from 25,609 Thai crossbred Holsteins between 1990 and 2008. Mean test-day milk yield ranged from 12.6 kg for cows with <87.5% Holstein genetics to 14.4 kg for cows with ≥93.7% Holstein genetics. Daily temperature and humidity data from 26 provincial weather stations were used to calculate a temperature-humidity index (THI). Test-day milk yield varied little with THI for first parity except above a THI of 82 for cows with ≥93.7% Holstein genetics. For third parity, test-day milk yield started to decline after a THI of 74 for cows with ≥87.5% Holstein genetics and declined more rapidly after a THI of 82. A repeatability test-day model with parities as correlated traits was used to estimate heat stress parameters; fixed effects included herd-test month-test year and breed groups, days in milk, calving age, and parity; random effects included 2 additive genetic effects, regular and heat stress, and 2 permanent environment, regular and heat stress. The threshold for effect of heat stress on test-day milk yield was set to a THI of 80. All variance component estimates increased with parity; the largest increases were found for effects associated with heat stress. In particular, genetic variance associated with heat stress quadrupled from first to third parity, whereas permanent environmental variance only doubled. However, permanent environmental variance for heat stress was at least 10 times larger than genetic variance. Genetic correlations among parities for additive effects without heat stress considered ranged from 0.88 to 0.96. Genetic correlations among parities for additive effects of heat stress ranged from 0.08 to 0.22, and genetic correlations between effects regular and heat stress effects ranged

  12. Modulus and yield stress of drawn LDPE

    NASA Astrophysics Data System (ADS)

    Thavarungkul, Nandh

    Modulus and yield stress were investigated in drawn low density polyethylene (LDPE) film. Uniaxially drawn polymeric films usually show high values of modulus and yield stress, however, studies have normally only been conducted to identify the structural features that determine modulus. In this study small-angle x-ray scattering (SAXS), thermal shrinkage, birefringence, differential scanning calorimetry (DSC), and dynamic mechanical thermal analysis (DMTA) were used to examine, directly and indirectly, the structural features that determine both modulus and yield stress, which are often closely related in undrawn materials. Shish-kebab structures are proposed to account for the mechanical properties in drawn LDPE. The validity of this molecular/morphological model was tested using relationships between static mechanical data and structural and physical parameters. In addition, dynamic mechanical results are also in line with static data in supporting the model. In the machine direction (MD), "shish" and taut tie molecules (TTM) anchored in the crystalline phase account for E; whereas crystal lamellae with contributions from "shish" and TTM determine yield stress. In the transverse direction (TD), the crystalline phase plays an important roll in both modulus and yield stress. Modulus is determined by crystal lamellae functioning as platelet reinforcing elements in the amorphous matrix with an additional contributions from TTM and yield stress is determined by the crystal lamellae's resistance to deformation.

  13. Biochar application increased the growth and yield and reduced cadmium in drought stressed wheat grown in an aged contaminated soil.

    PubMed

    Abbas, Tahir; Rizwan, Muhammad; Ali, Shafaqat; Adrees, Muhammad; Mahmood, Abid; Zia-Ur-Rehman, Muhammad; Ibrahim, Muhammad; Arshad, Muhammad; Qayyum, Muhammad Farooq

    2018-02-01

    Cadmium (Cd) and drought stress in plants is a worldwide problem, whereas little is known about the effect of biochar (BC) under combined Cd and drought stress. The current study was conducted to determine the impact of BC on Cd uptake in wheat sown in Cd-contaminated soil under drought stress. Wheat was grown in a soil after incubating the soil for 15 days with three levels of BC (0%, 3.0% and 5.0% w/w). Three levels of drought stress (well-watered, mild drought and severe drought containing 70%, 50%, and 35% of soil water holding capacity respectively) were applied to 45-d-old wheat plants. Drought stress decreased plant height, spike length, chlorophyll contents, gas exchange parameters, root and shoot dry biomasses and grain yields. Drought stress also caused oxidative stress and decreased the antioxidant enzymes activities whereas increased the Cd concentration in plants. Biochar increased morphological and physiological parameters of wheat under combined drought and Cd stress and reduced the oxidative stress and Cd contents and increased antioxidant enzymes activities. The decrease in Cd concentration with BC application in drought-stressed plant might be attributed to BC-induced increase in crop biomass production and reduction in oxidative stress. These results indicate that BC could be used as an amendment in metal contaminated soil for improving wheat growth and reducing Cd concentrations under semiarid conditions. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Influence of polymer charge on the shear yield stress of silica aggregated with adsorbed cationic polymers.

    PubMed

    Zhou, Ying; Yu, Hai; Wanless, Erica J; Jameson, Graeme J; Franks, George V

    2009-08-15

    Flocs were produced by adding three cationic polymers (10% charge density, 3.0x10(5) g/mol molecular weight; 40% charge density, 1.1x10(5) g/mol molecular weight; and 100% charge density, 1.2x10(5) g/mol molecular weight) to 90 nm diameter silica particles. The shear yield stresses of the consolidated sediment beds from settled and centrifuged flocs were determined via the vane technique. The polymer charge density plays an important role in influencing the shear yield stresses of sediment beds. The shear yield stresses of sediment beds from flocs induced by the 10% charged polymer were observed to increase with an increase in polymer dose, initial solid concentration and background electrolyte concentration at all volume fractions. In comparison, polymer dose has a marginal effect on the shear yield stresses of sediment beds from flocs induced by the 40% and 100% charged polymers. The shear yield stresses of sediments from flocs induced by the 40% charged polymer are independent of salt concentration whereas the addition of salt decreases the shear yield stresses of sediments from flocs induced by the 100% charged polymer. When flocculated at the optimum dose for each polymer (12 mg/g silica for the 10% charged polymer at 0.03 M NaCl, 12 mg/g for 40% and 2 mg/g for 100%), shear yield stress increases as polymer charge increases. The effects observed are related to the flocculation mechanism (bridging, patch attraction or charge neutralisation) and the magnitude of the adhesive force. Comparison of shear and compressive yield stresses show that the network is only slightly weaker in shear than in compression. This is different than many other systems (mainly salt and pH coagulation) which have shear yield stress much less than compressive yield stress. The existing models relating the power law exponent of the volume fraction dependence of the shear yield stress to the network fractal structure are not satisfactory to predict all the experimental behaviour.

  15. Increasing crop diversity mitigates weather variations and improves yield stability.

    PubMed

    Gaudin, Amélie C M; Tolhurst, Tor N; Ker, Alan P; Janovicek, Ken; Tortora, Cristina; Martin, Ralph C; Deen, William

    2015-01-01

    Cropping sequence diversification provides a systems approach to reduce yield variations and improve resilience to multiple environmental stresses. Yield advantages of more diverse crop rotations and their synergistic effects with reduced tillage are well documented, but few studies have quantified the impact of these management practices on yields and their stability when soil moisture is limiting or in excess. Using yield and weather data obtained from a 31-year long term rotation and tillage trial in Ontario, we tested whether crop rotation diversity is associated with greater yield stability when abnormal weather conditions occur. We used parametric and non-parametric approaches to quantify the impact of rotation diversity (monocrop, 2-crops, 3-crops without or with one or two legume cover crops) and tillage (conventional or reduced tillage) on yield probabilities and the benefits of crop diversity under different soil moisture and temperature scenarios. Although the magnitude of rotation benefits varied with crops, weather patterns and tillage, yield stability significantly increased when corn and soybean were integrated into more diverse rotations. Introducing small grains into short corn-soybean rotation was enough to provide substantial benefits on long-term soybean yields and their stability while the effects on corn were mostly associated with the temporal niche provided by small grains for underseeded red clover or alfalfa. Crop diversification strategies increased the probability of harnessing favorable growing conditions while decreasing the risk of crop failure. In hot and dry years, diversification of corn-soybean rotations and reduced tillage increased yield by 7% and 22% for corn and soybean respectively. Given the additional advantages associated with cropping system diversification, such a strategy provides a more comprehensive approach to lowering yield variability and improving the resilience of cropping systems to multiple environmental

  16. Increasing Crop Diversity Mitigates Weather Variations and Improves Yield Stability

    PubMed Central

    Gaudin, Amélie C. M.; Tolhurst, Tor N.; Ker, Alan P.; Janovicek, Ken; Tortora, Cristina; Martin, Ralph C.; Deen, William

    2015-01-01

    Cropping sequence diversification provides a systems approach to reduce yield variations and improve resilience to multiple environmental stresses. Yield advantages of more diverse crop rotations and their synergistic effects with reduced tillage are well documented, but few studies have quantified the impact of these management practices on yields and their stability when soil moisture is limiting or in excess. Using yield and weather data obtained from a 31-year long term rotation and tillage trial in Ontario, we tested whether crop rotation diversity is associated with greater yield stability when abnormal weather conditions occur. We used parametric and non-parametric approaches to quantify the impact of rotation diversity (monocrop, 2-crops, 3-crops without or with one or two legume cover crops) and tillage (conventional or reduced tillage) on yield probabilities and the benefits of crop diversity under different soil moisture and temperature scenarios. Although the magnitude of rotation benefits varied with crops, weather patterns and tillage, yield stability significantly increased when corn and soybean were integrated into more diverse rotations. Introducing small grains into short corn-soybean rotation was enough to provide substantial benefits on long-term soybean yields and their stability while the effects on corn were mostly associated with the temporal niche provided by small grains for underseeded red clover or alfalfa. Crop diversification strategies increased the probability of harnessing favorable growing conditions while decreasing the risk of crop failure. In hot and dry years, diversification of corn-soybean rotations and reduced tillage increased yield by 7% and 22% for corn and soybean respectively. Given the additional advantages associated with cropping system diversification, such a strategy provides a more comprehensive approach to lowering yield variability and improving the resilience of cropping systems to multiple environmental

  17. Yield stress materials in soft condensed matter

    NASA Astrophysics Data System (ADS)

    Bonn, Daniel; Denn, Morton M.; Berthier, Ludovic; Divoux, Thibaut; Manneville, Sébastien

    2017-07-01

    A comprehensive review is presented of the physical behavior of yield stress materials in soft condensed matter, which encompasses a broad range of materials from colloidal assemblies and gels to emulsions and non-Brownian suspensions. All these disordered materials display a nonlinear flow behavior in response to external mechanical forces due to the existence of a finite force threshold for flow to occur: the yield stress. Both the physical origin and rheological consequences associated with this nonlinear behavior are discussed and an overview is given of experimental techniques available to measure the yield stress. Recent progress is discussed concerning a microscopic theoretical description of the flow dynamics of yield stress materials, emphasizing, in particular, the role played by relaxation time scales, the interplay between shear flow and aging behavior, the existence of inhomogeneous shear flows and shear bands, wall slip, and nonlocal effects in confined geometries.

  18. The compensation effects of physiology and yield in cotton after drought stress.

    PubMed

    Niu, Jing; Zhang, Siping; Liu, Shaodong; Ma, Huijuan; Chen, Jing; Shen, Qian; Ge, Changwei; Zhang, Xiaomeng; Pang, Chaoyou; Zhao, Xinhua

    The objective of this study was to investigate the root growth compensatory effects and cotton yield under drought stress. The results indicate that the root dry weight, boll weight, and cotton yield increased in both the drought-resistant cultivar (CCRI-45) and the drought-sensitive cultivar (CCRI-60). Compensation effects were exhibited under the three-day drought stress treatment at a soil relative water content (SRWC) of 60% and 45% during the seedling stage, and flowering and boll-forming stage over two years. The yield of the drought-resistant cultivar (CCRI-45) was higher than the control, however, following the six-day 45% SRWC drought treatments, the yield of the drought-sensitive cultivar (CCRI-60) was lower than the control. The soluble sugar content, proline content, superoxide dismutase (SOD) activity, and peroxidase (POD) activity of the roots increased under drought stress and then decreased after re-watering, although the values remained higher than those of the controls for a short period. These physiological measures may represent stress reactions and thus may not indicate factors that result in compensation effects. However, catalase (CAT) activity and gibberellic acid (GA) content of the roots decreased under drought stress. After re-watering, the CAT activity and the GA content increased and were significantly higher than those of the controls under the six-day 60% SRWC and 45% SRWC drought treatments. The abscisic acid (ABA) content of the roots increased under drought stress. After re-watering, the ABA content decreased to a lower level under the three and six-day 60% SRWC and 45% SRWC drought treatments than in the controls. According to an analysis of various indicators, the interaction between ABA and GA signals may play an important role in root growth compensatory effects. In summary, the results demonstrate that moderate drought stress is beneficial to root growth and yield. This conclusion is of great significance to improving our

  19. Separating heat stress from moisture stress: analyzing yield response to high temperature in irrigated maize

    NASA Astrophysics Data System (ADS)

    Carter, Elizabeth K.; Melkonian, Jeff; Riha, Susan J.; Shaw, Stephen B.

    2016-09-01

    Several recent studies have indicated that high air temperatures are limiting maize (Zea mays L.) yields in the US Corn Belt and project significant yield losses with expected increases in growing season temperatures. Further work has suggested that high air temperatures are indicative of high evaporative demand, and that decreases in maize yields which correlate to high temperatures and vapor pressure deficits (VPD) likely reflect underlying soil moisture limitations. It remains unclear whether direct high temperature impacts on yields, independent of moisture stress, can be observed under current temperature regimes. Given that projected high temperature and moisture may not co-vary the same way as they have historically, quantitative analyzes of direct temperature impacts are critical for accurate yield projections and targeted mitigation strategies under shifting temperature regimes. To evaluate yield response to above optimum temperatures independent of soil moisture stress, we analyzed climate impacts on irrigated maize yields obtained from the National Corn Growers Association (NCGA) corn yield contests for Nebraska, Kansas and Missouri. In irrigated maize, we found no evidence of a direct negative impact on yield by daytime air temperature, calculated canopy temperature, or VPD when analyzed seasonally. Solar radiation was the primary yield-limiting climate variable. Our analyses suggested that elevated night temperature impacted yield by increasing rates of phenological development. High temperatures during grain-fill significantly interacted with yields, but this effect was often beneficial and included evidence of acquired thermo-tolerance. Furthermore, genetics and management—information uniquely available in the NCGA contest data—explained more yield variability than climate, and significantly modified crop response to climate. Thermo-acclimation, improved genetics and changes to management practices have the potential to partially or completely

  20. Root xylem plasticity to improve water use and yield in water-stressed soybean

    PubMed Central

    Prince, Silvas J.; Murphy, Mackensie; Durnell, Lorellin A.; Shannon, J. Grover

    2017-01-01

    Abstract We tested the hypothesis that increasing the number of metaxylem vessels would enhance the efficiency of water uptake in soybean (Glycine max) and decrease the yield gap in water-limited environments. A panel of 41 soybean accessions was evaluated in greenhouse, rainout shelter, and rain-fed field environments. The metaxylem number influenced the internal capture of CO2 and improved stomatal conductance, enhancing water uptake/use in soybeans exposed to stress during the reproductive stage. We determined that other root anatomical features, such as cortex cell area and the percentage of stele that comprised cortical cells, also affected seed yield under similar growth parameters. Seed yield was also impacted by pod retention rates under drought stress (24–80 pods/plant). We surmise that effective biomass allocation, that is, the transport of available photosynthates to floral structures at late reproductive growth stages (R6–R7), enables yield protection under drought stress. A mesocosm study of contrasting lines for yield under drought stress and root anatomical features revealed that increases in metaxylem number as an adaptation to drought in the high-yielding lines improved root hydraulic conductivity, which reduced the metabolic cost of exploring water in deeper soil strata and enhanced water transport. This allowed the maintenance of shoot physiological processes under water-limited conditions. PMID:28064176

  1. Mobility of Yield-Stress Fluids on Lubricant-Impregnated Surface

    NASA Astrophysics Data System (ADS)

    Rapoport, Leonid; Solomon, Brian; Varanasi, Kripa; Varanasi Research Group Team

    2017-11-01

    Assuring the flow of yield-stress fluids is an essential problem for various industries such as consumer products, health care, and energy. Elimination of wall-induced pinning forces can potentially save power and cleaning costs as well as enable the flow of yield-stress fluids in channels previously considered too narrow. Lubricant-Impregnated Surfaces (LIS) have been demonstrated to change the dynamic behavior of yield-stress fluids and enable them to move as bulk without shearing at all. However, despite the wide applicability of this technology and its general appeal, the fundamental principles governing the performance of yield stress fluids on LIS have not yet been fully explained. In this work, we explore the mobility of yield stress fluids on a wide range of LIS, and explain the connection between macroscale behavior and the microscale properties of the LIS. Specifically, we show a striking difference in mobility between an LIS that contains a lubricant which fully spreads on the rough micro-features of the surface, and an LIS that contains a lubricant which only imbibes these features but does spread over them

  2. Hydrostatic Stress Effect On the Yield Behavior of Inconel 100

    NASA Technical Reports Server (NTRS)

    Allen, Phillip A.; Wilson, Christopher D.

    2002-01-01

    Classical metal plasticity theory assumes that hydrostatic stress has no effect on the yield and postyield behavior of metals. Recent reexaminations of classical theory have revealed a significant effect of hydrostatic stress on the yield behavior of notched geometries. New experiments and nonlinear finite element analyses (FEA) of Inconel 100 (IN 100) equal-arm bend and double-edge notch tension (DENT) test specimens have revealed the effect of internal hydrostatic tensile stresses on yielding. Nonlinear FEA using the von Mises (yielding is independent of hydrostatic stress) and the Drucker-Prager (yielding is linearly dependent on hydrostatic stress) yield functions was performed. In all test cases, the von Mises constitutive model, which is independent of hydrostatic pressure, overestimated the load for a given displacement or strain. Considering the failure displacements or strains, the Drucker-Prager FEMs predicted loads that were 3% to 5% lower than the von Mises values. For the failure loads, the Drucker Prager FEMs predicted strains that were 20% to 35% greater than the von Mises values. The Drucker-Prager yield function seems to more accurately predict the overall specimen response of geometries with significant internal hydrostatic stress influence.

  3. Effect of the plate surface characteristics and gap height on yield stresses of a magnetorheological fluid

    NASA Astrophysics Data System (ADS)

    Jonkkari, I.; Kostamo, E.; Kostamo, J.; Syrjala, S.; Pietola, M.

    2012-07-01

    Effects of the plate material, surface roughness and measuring gap height on static and dynamic yield stresses of a magnetorheological (MR) fluid were investigated with a commercial plate-plate magnetorheometer. Magnetic and non-magnetic plates with smooth (Ra ˜ 0.3 μm) and rough (Ra ˜ 10 μm) surface finishes were used. It was shown by Hall probe measurements and finite element simulations that the use of magnetic plates or higher gap heights increases the level of magnetic flux density and changes the shape of the radial flux density profile. The yield stress increase caused by these factors was determined and subtracted from the measured values in order to examine only the effect of the wall characteristics or the gap height. Roughening of the surfaces offered a significant increase in the yield stresses for non-magnetic plates. With magnetic plates the yield stresses were higher to start with, but roughening did not increase them further. A significant part of the difference in measured stresses between rough non-magnetic and magnetic plates was caused by changes in magnetic flux density rather than by better contact of the particles to the plate surfaces. In a similar manner, an increase in gap height from 0.25 to 1.00 mm can lead to over 20% increase in measured stresses due to changes in the flux density profile. When these changes were compensated the dynamic yield stresses generally remained independent of the gap height, even in the cases where it was obvious that the wall slip was present. This suggests that with MR fluids the wall slip cannot be reliably detected by comparison of flow curves measured at different gap heights.

  4. A novel rheometer design for yield stress fluids

    Treesearch

    Joseph R. Samaniuk; Timothy W. Shay; Thatcher W. Root; Daniel J. Klingenberg; C. Tim Scott

    2014-01-01

    An inexpensive, rapid method for measuring the rheological properties of yield stress fluids is described and tested. The method uses an auger that does not rotate during measurements, and avoids material and instrument-related difficulties, for example, wall slip and the presence of large particles, associated with yield stress fluids. The method can be used...

  5. Yield, quality and biochemical properties of various strawberry cultivars under water stress.

    PubMed

    Adak, Nafiye; Gubbuk, Hamide; Tetik, Nedim

    2018-01-01

    Although strawberry (Fragaria x ananassa Duch.) species are sensitive to abiotic stress conditions, some cultivars are known to be tolerant to different environmental conditions. We examined the response of different strawberry cultivars to water stress conditions in terms of yield, quality and biochemical features. The trial was conducted under two different irrigation regimes: in grow bags containing cocopeat (control, 30%; water stress, 15% drainage) with four different cultivars (Camarosa, Albion, Amiga and Rubygem). Fruit weight declined by 59.72% and the yield per unit area by 63.62% under water stress conditions as compared to control. Albion and Rubygem were found to be more tolerant and Amiga the most sensitive in terms of yield under stress conditions. Water stress increased all biochemical features in fruits such as total phenol, total anthocyanin, antioxidant activity and sugar contents. Among the cultivars, glucose and fructose was higher in Albion. Considering the rise in global warming, identification of resistant and tolerant cultivars to stress conditions are crucial for future breeding programmes. Our results showed that some of the fruit's physical features were affected negatively by stress conditions whereas many of the biochemical features such as total anthocyanin content, total phenolic content and antioxidant activity were positively modulated. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  6. Yield Stress Reduction of DWPF Melter Feed Slurries

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stone, M.E.; Smith, M.E.

    2007-07-01

    The Defense Waste Processing Facility (DWPF) at the Savannah River Site vitrifies High Level Waste for repository internment. The process consists of three major steps: waste pretreatment, vitrification, and canister decontamination/sealing. The HLW consists of insoluble metal hydroxides and soluble sodium salts. The pretreatment process acidifies the sludge with nitric and formic acids, adds the glass formers as glass frit, then concentrates the resulting slurry to approximately 50 weight percent (wt%) total solids. This slurry is fed to the joule-heated melter where the remaining water is evaporated followed by calcination of the solids and conversion to glass. The Savannah Rivermore » National Laboratory (SRNL) is currently assisting DWPF efforts to increase throughput of the melter. As part of this effort, SRNL has investigated methods to increase the solids content of the melter feed to reduce the heat load required to complete the evaporation of water and allow more of the energy available to calcine and vitrify the waste. The process equipment in the facility is fixed and cannot process materials with high yield stresses, therefore increasing the solids content will require that the yield stress of the melter feed slurries be reduced. Changing the glass former added during pretreatment from an irregularly shaped glass frit to nearly spherical beads was evaluated. The evaluation required a systems approach which included evaluations of the effectiveness of beads in reducing the melter feed yield stress as well as evaluations of the processing impacts of changing the frit morphology. Processing impacts of beads include changing the settling rate of the glass former (which effects mixing and sampling of the melter feed slurry and the frit addition equipment) as well as impacts on the melt behavior due to decreased surface area of the beads versus frit. Beads were produced from the DWPF process frit by fire polishing. The frit was allowed to free fall through

  7. Maize ARGOS1 (ZAR1) transgenic alleles increase hybrid maize yield.

    PubMed

    Guo, Mei; Rupe, Mary A; Wei, Jun; Winkler, Chris; Goncalves-Butruille, Marymar; Weers, Ben P; Cerwick, Sharon F; Dieter, Jo Ann; Duncan, Keith E; Howard, Richard J; Hou, Zhenglin; Löffler, Carlos M; Cooper, Mark; Simmons, Carl R

    2014-01-01

    Crop improvement for yield and drought tolerance is challenging due to the complex genetic nature of these traits and environmental dependencies. This study reports that transgenic over-expression of Zea mays AR GOS1 (ZAR1) enhanced maize organ growth, grain yield, and drought-stress tolerance. The ZAR1 transgene exhibited environmental interactions, with yield increase under Temperate Dry and yield reduction under Temperate Humid or High Latitude environments. Native ZAR1 allele variation associated with drought-stress tolerance. Two founder alleles identified in the mid-maturity germplasm of North America now predominate in Pioneer's modern breeding programme, and have distinct proteins, promoters and expression patterns. These two major alleles show heterotic group partitioning, with one predominant in Pioneer's female and the other in the male heterotic groups, respectively. These two alleles also associate with favourable crop performance when heterozygous. Allele-specific transgene testing showed that, of the two alleles discussed here, each allele differed in their impact on yield and environmental interactions. Moreover, when transgenically stacked together the allelic pair showed yield and environmental performance advantages over either single allele, resembling heterosis effects. This work demonstrates differences in transgenic efficacy of native alleles and the differences reflect their association with hybrid breeding performance.

  8. Maize ARGOS1 (ZAR1) transgenic alleles increase hybrid maize yield

    PubMed Central

    Guo, Mei

    2014-01-01

    Crop improvement for yield and drought tolerance is challenging due to the complex genetic nature of these traits and environmental dependencies. This study reports that transgenic over-expression of Zea mays ARGOS1 (ZAR1) enhanced maize organ growth, grain yield, and drought-stress tolerance. The ZAR1 transgene exhibited environmental interactions, with yield increase under Temperate Dry and yield reduction under Temperate Humid or High Latitude environments. Native ZAR1 allele variation associated with drought-stress tolerance. Two founder alleles identified in the mid-maturity germplasm of North America now predominate in Pioneer’s modern breeding programme, and have distinct proteins, promoters and expression patterns. These two major alleles show heterotic group partitioning, with one predominant in Pioneer’s female and the other in the male heterotic groups, respectively. These two alleles also associate with favourable crop performance when heterozygous. Allele-specific transgene testing showed that, of the two alleles discussed here, each allele differed in their impact on yield and environmental interactions. Moreover, when transgenically stacked together the allelic pair showed yield and environmental performance advantages over either single allele, resembling heterosis effects. This work demonstrates differences in transgenic efficacy of native alleles and the differences reflect their association with hybrid breeding performance. PMID:24218327

  9. Postpartum endocrine activities, metabolic attributes and milk yield are influenced by thermal stress in crossbred dairy cows

    NASA Astrophysics Data System (ADS)

    Ihsanullah; Qureshi, Muhammad Subhan; Suhail, Syed Muhammad; Akhtar, Sohail; Khan, Rifat Ullah

    2017-09-01

    This study was conducted on 30 freshly parturated multiparous crossbred dairy cows possessing three levels of Holstein Frisian genetic makeup (62.5, 75.0, and 87.5%). Data on temperature humidity index (THI) were classified into comfortable (≤ 71), mild stress (72-79), moderate stress (80-89), and stressful (≥90) zone. Results showed that serum cortisol concentration increased significantly ( P < 0.05) in cows during stressful condition irrespective of genetic makeup compared to the other zones. Daily milk yield (DMY) was significantly ( P < 0.05) lower in cows during stressful condition. Triglyceride was significantly higher in cows with genetic makeup 87.5% compared to the others, while total serum protein was significantly ( P < 0.05) higher in cows during both moderate and stressful conditions. The mean concentration of cortisol and protein increased linearly from comfort to the stressful condition, while mean serum triglyceride, glucose, progesterone (P4), and luteinizing hormone (LH) decreased by moving from comfort to stressful conditions. Results also indicated that higher cortisol level in higher grade crossbred cows was adversely associated with LH concentration and milk yield under thermal stress conditions. Greater triglyceride in high-grade crossbred (87.5%) cows indicates higher fat mobilization reflecting a negative energy balance. We concluded that heat stress increased blood cortisol and protein, and reduced milk yield in dairy cows irresptive of the genetic makeup. In addition, there was no significant difference in blood metabolites and daily milk yield in the different levels of genetic makeup cows.

  10. Non-structural carbohydrate partitioning in grass stems: a target to increase yield stability, stress tolerance, and biofuel production.

    PubMed

    Slewinski, Thomas L

    2012-08-01

    A dramatic change in agricultural crops is needed in order to keep pace with the demands of an increasing human population, exponential need for renewable fuels, and uncertain climatic changes. Grasses make up the vast majority of agricultural commodities. How these grasses capture, transport, and store carbohydrates underpins all aspects of crop productivity. Sink-source dynamics within the plant direct how much, where, and when carbohydrates are allocated, as well as determine the harvestable tissue. Carbohydrate partitioning can limit the yield capacity of these plants, thus offering a potential target for crop improvement. Grasses have the ability to buffer this sink-source interaction by transiently storing carbohydrates in stem tissue when production from the source is greater than whole-plant demand. These reserves improve yield stability in grain crops by providing an alternative source when photosynthetic capacity is reduced during the later phases of grain filling, or during periods of environmental and biotic stresses. Domesticated grasses such as sugarcane and sweet sorghum have undergone selection for high accumulation of stem carbohydrates, which serve as the primary sources of sugars for human and animal consumption, as well as ethanol production for fuel. With the enormous expectations placed on agricultural production in the near future, research into carbohydrate partitioning in grasses is essential for maintaining and increasing yields in grass crops. This review highlights the current knowledge of non-structural carbohydrate dynamics in grass stems and discusses the impacts of stem reserves in essential agronomic grasses.

  11. Hydrostatic Stress Effect on the Yield Behavior of Inconel 100

    NASA Technical Reports Server (NTRS)

    Allen, Phillip A.; Wilson, Christopher D.

    2003-01-01

    Classical metal plasticity theory assumes that hydrostatic stress has negligible effect on the yield and postyield behavior of metals. Recent reexaminations of classical theory have revealed a significant effect of hydrostatic stress on the yield behavior of various geometries. Fatigue tests and nonlinear finite element analyses (FEA) of Inconel 100 (IN100) equal-arm bend specimens and new monotonic tests and nonlinear finite element analyses of IN100 smooth tension, smooth compression, and double-edge notch tension (DENT) test specimens have revealed the effect of internal hydrostatic tensile stresses on yielding. Nonlinear FEA using the von Mises (yielding is independent of hydrostatic stress) and the Drucker-Prager (yielding is linearly dependent on hydrostatic stress) yield functions were performed. A new FEA constitutive model was developed that incorporates a pressure-dependent yield function with combined multilinear kinematic and multilinear isotropic hardening using the ABAQUS user subroutine (UMAT) utility. In all monotonic tensile test cases, the von Mises constitutive model, overestimated the load for a given displacement or strain. Considering the failure displacements or strains for the DENT specimen, the Drucker-Prager FEM s predicted loads that were approximately 3% lower than the von Mises values. For the failure loads, the Drucker Prager FEM s predicted strains that were up to 35% greater than the von Mises values. Both the Drucker-Prager model and the von Mises model performed equally-well in simulating the equal-arm bend fatigue test.

  12. Postpartum endocrine activities, metabolic attributes and milk yield are influenced by thermal stress in crossbred dairy cows.

    PubMed

    Ihsanullah; Qureshi, Muhammad Subhan; Suhail, Syed Muhammad; Akhtar, Sohail; Khan, Rifat Ullah

    2017-09-01

    This study was conducted on 30 freshly parturated multiparous crossbred dairy cows possessing three levels of Holstein Frisian genetic makeup (62.5, 75.0, and 87.5%). Data on temperature humidity index (THI) were classified into comfortable (≤ 71), mild stress (72-79), moderate stress (80-89), and stressful (≥90) zone. Results showed that serum cortisol concentration increased significantly (P < 0.05) in cows during stressful condition irrespective of genetic makeup compared to the other zones. Daily milk yield (DMY) was significantly (P < 0.05) lower in cows during stressful condition. Triglyceride was significantly higher in cows with genetic makeup 87.5% compared to the others, while total serum protein was significantly (P < 0.05) higher in cows during both moderate and stressful conditions. The mean concentration of cortisol and protein increased linearly from comfort to the stressful condition, while mean serum triglyceride, glucose, progesterone (P 4 ), and luteinizing hormone (LH) decreased by moving from comfort to stressful conditions. Results also indicated that higher cortisol level in higher grade crossbred cows was adversely associated with LH concentration and milk yield under thermal stress conditions. Greater triglyceride in high-grade crossbred (87.5%) cows indicates higher fat mobilization reflecting a negative energy balance. We concluded that heat stress increased blood cortisol and protein, and reduced milk yield in dairy cows irresptive of the genetic makeup. In addition, there was no significant difference in blood metabolites and daily milk yield in the different levels of genetic makeup cows.

  13. YIELD STRESS REDUCTION OF DWPF MELTER FEED SLURRIES

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stone, M; Michael02 Smith, M

    2006-12-28

    The Defense Waste Processing Facility (DWPF) at the Savannah River Site vitrifies High Level Waste for repository internment. The process consists of three major steps: waste pretreatment, vitrification, and canister decontamination/sealing. The HLW consists of insoluble metal hydroxides (primarily iron, aluminum, magnesium, manganese, and uranium) and soluble sodium salts (carbonate, hydroxide, nitrite, nitrate, sulfate). The pretreatment process acidifies the sludge with nitric and formic acids, adds the glass formers as glass frit, then concentrates the resulting slurry to approximately 50 weight percent (wt%) total solids. This slurry is fed to the joule-heated melter where the remaining water is evaporated followedmore » by calcination of the solids and conversion to glass. The Savannah River National Laboratory (SRNL) is currently assisting DWPF efforts to increase throughput of the melter. As part of this effort, SRNL has investigated methods to increase the solids content of the melter feed to reduce the heat load required to complete the evaporation of water and allow more of the energy available to calcine and vitrify the waste. The process equipment in the facility is fixed and cannot process materials with high yield stresses, therefore increasing the solids content will require that the yield stress of the melter feed slurries be reduced. Changing the glass former added during pretreatment from an irregularly shaped glass frit to nearly spherical beads was evaluated. The evaluation required a systems approach which included evaluations of the effectiveness of beads in reducing the melter feed yield stress as well as evaluations of the processing impacts of changing the frit morphology. Processing impacts of beads include changing the settling rate of the glass former (which effects mixing and sampling of the melter feed slurry and the frit addition equipment) as well as impacts on the melt behavior due to decreased surface area of the beads versus

  14. Development of buoyant currents in yield stress fluids

    NASA Astrophysics Data System (ADS)

    Rossi, P.; Karimfazli, I.

    2017-11-01

    Infinitesimal perturbations are known to decay in a motionless yield stress fluid. We present experimental evidence to reveal other mechanisms promoting free advection from a motionless background state. Development of natural convection in a cavity with differentially heated side-walls is investigated as a benchmark. Velocity and temperature fields are measured using particle image velocimetry/thermometry. We examine time evolution of the flow, compare experimental findings with theoretical predictions and comment on the striking features brought about by the yield stress.

  15. Preferential paths in yield stress fluid flow through a porous medium

    NASA Astrophysics Data System (ADS)

    Guasto, Jeffrey; Waisbord, Nicolas; Stoop, Norbert; Dunkel, Jörn

    2016-11-01

    A broad range of biological, geological, and industrial materials with complex rheological properties are subjected to flow through porous media in applications ranging from oil recovery to food manufacturing. In this experimental study, we examine the flow of a model yield stress fluid (Carbopol micro-gel) through a quasi-2D porous medium, fabricated in a microfluidic channel. The flow is driven by applying a precisely-controlled pressure gradient and measured by particle tracking velocimetry, and our observations are complemented by a pore-network model of the yield stress fluid flow. While remaining unyielded at small applied pressure, the micro-gel begins to yield at a critical pressure gradient, exhibiting a single preferential flow path that percolates through the porous medium. As the applied pressure gradient increases, we observe a subsequent coarsening and invasion of the yielded, fluidized network. An examination of both the yielded network topology and pore-scale flow reveal that two cooperative phenomena are involved in sculpting the preferential flow paths: (1) the geometry of the porous microstructure, and (2) the adhesive surface interactions between the micro-gel and substrate. NSF CBET-1511340.

  16. Modelling climate change impacts on viticultural yield, phenology and stress conditions in Europe.

    PubMed

    Fraga, Helder; García de Cortázar Atauri, Iñaki; Malheiro, Aureliano C; Santos, João A

    2016-11-01

    Viticulture is a key socio-economic sector in Europe. Owing to the strong sensitivity of grapevines to atmospheric factors, climate change may represent an important challenge for this sector. This study analyses viticultural suitability, yield, phenology, and water and nitrogen stress indices in Europe, for present climates (1980-2005) and future (2041-2070) climate change scenarios (RCP4.5 and 8.5). The STICS crop model is coupled with climate, soil and terrain databases, also taking into account CO 2 physiological effects, and simulations are validated against observational data sets. A clear agreement between simulated and observed phenology, leaf area index, yield and water and nitrogen stress indices, including the spatial differences throughout Europe, is shown. The projected changes highlight an extension of the climatic suitability for grapevines up to 55°N, which may represent the emergence of new winemaking regions. Despite strong regional heterogeneity, mean phenological timings (budburst, flowering, veraison and harvest) are projected to undergo significant advancements (e.g. budburst/harvest can be >1 month earlier), with implications also in the corresponding phenophase intervals. Enhanced dryness throughout Europe is also projected, with severe water stress over several regions in southern regions (e.g. southern Iberia and Italy), locally reducing yield and leaf area. Increased atmospheric CO 2 partially offsets dryness effects, promoting yield and leaf area index increases in central/northern Europe. Future biomass changes may lead to modifications in nitrogen demands, with higher stress in northern/central Europe and weaker stress in southern Europe. These findings are critical decision support systems for stakeholders from the European winemaking sector. © 2016 John Wiley & Sons Ltd.

  17. Nanoscale Roughness of Faults Explained by the Scale-Dependent Yield Stress of Geologic Materials

    NASA Astrophysics Data System (ADS)

    Thom, C.; Brodsky, E. E.; Carpick, R. W.; Goldsby, D. L.; Pharr, G.; Oliver, W.

    2017-12-01

    Despite significant differences in their lithologies and slip histories, natural fault surfaces exhibit remarkably similar scale-dependent roughness over lateral length scales spanning 7 orders of magnitude, from microns to tens of meters. Recent work has suggested that a scale-dependent yield stress may result in such a characteristic roughness, but experimental evidence in favor of this hypothesis has been lacking. We employ an atomic force microscope (AFM) operating in intermittent-contact mode to map the topography of the Corona Heights fault surface. Our experiments demonstrate that the Corona Heights fault exhibits isotropic self-affine roughness with a Hurst exponent of 0.75 +/- 0.05 at all wavelengths from 60 nm to 10 μm. If yield stress controls roughness, then the roughness data predict that yield strength varies with length scale as λ-0.25 +/ 0.05. To test the relationship between roughness and yield stress, we conducted nanoindentation tests on the same Corona Heights sample and a sample of the Yair Fault, a carbonate fault surface that has been previously characterized by AFM. A diamond Berkovich indenter tip was used to indent the samples at a nominally constant strain rate (defined as the loading rate divided by the load) of 0.2 s-1. The continuous stiffness method (CSM) was used to measure the indentation hardness (which is proportional to yield stress) and the elastic modulus of the sample as a function of depth in each test. For both samples, the yield stress decreases with increasing size of the indents, a behavior consistent with that observed for many engineering materials and recently for other geologic materials such as olivine. The magnitude of this "indentation size effect" is best described by a power-law with exponents of -0.12 +/- 0.06 and -0.18 +/- 0.08 for the Corona Heights and Yair Faults, respectively. These results demonstrate a link between surface roughness and yield stress, and suggest that fault geometry is the physical

  18. An Update on Genetic Modification of Chickpea for Increased Yield and Stress Tolerance.

    PubMed

    Kumar, Manoj; Yusuf, Mohd Aslam; Nigam, Manisha; Kumar, Manoj

    2018-06-26

    Chickpea is a highly nutritious grain legume crop, widely appreciated as a health food, especially in the Indian subcontinent. The major constraints on chickpea production are biotic (Helicoverpa, bruchid, aphid, ascochyta) and abiotic (drought, heat, salt, cold) stresses, which reduce the yield by up to 90%. Various strategies like conventional breeding, molecular breeding, and modern plant breeding have been used to overcome these problems. Conventionally, breeding programs aim at development of varieties that combine maximum number of traits through inter-specific hybridization, wide hybridization, and hybridization involving more than two parents. Breeding is difficult in this crop because of its self-pollinating nature and limited genetic variation. Recent advances in in vitro culture and gene technologies offer unique opportunities to realize the full potential of chickpea production. However, as of date, no transgenic chickpea variety has been approved for cultivation in the world. In this review, we provide an update on the development of genetically modified chickpea plants, including those resistant to Helicoverpa armigera, Callosobruchus maculatus, Aphis craccivora, as well as to drought and salt stress. The genes utilized for development of resistance against pod borer, bruchid, aphid, drought, and salt tolerance, namely, Bt, alpha amylase inhibitor, ASAL, P5CSF129A, and P5CS, respectively, are discussed.

  19. Different Effects of NSF and PCE Superplasticizer on Adsorption, Dynamic Yield Stress and Thixotropy of Cement Pastes

    PubMed Central

    2018-01-01

    This study compares the differences and similarities of two types of superplasticizers—NSF (Naphthalene Sulfonate Formaldehyde) and PCE (PolyCarboxylate Ester)—in fresh cement paste systems, in terms of adsorption, dynamic yield stress, and thixotropic index. Results show that with either NSF or PCE addition, the more superplasticizer is added, the more it is adsorbed and the more it remains in the interstitial pore solution. The dynamic yield stress and thixotropic index also decrease with increasing addition the amount of either superplasticizer. However, NSF is less efficient in decreasing the dynamic yield stress than PCE. More importantly, the decreasing patterns of dynamic yield stress and thixotropic index are different with NSF and PCE additions; this is tied to the adsorption and dispersing mechanisms of these two types of superplasticizers. PMID:29710782

  20. Identification of Crowding Stress Tolerance Co-Expression Networks Involved in Sweet Corn Yield

    PubMed Central

    Choe, Eunsoo; Drnevich, Jenny; Williams, Martin M.

    2016-01-01

    Tolerance to crowding stress has played a crucial role in improving agronomic productivity in field corn; however, commercial sweet corn hybrids vary greatly in crowding stress tolerance. The objectives were to 1) explore transcriptional changes among sweet corn hybrids with differential yield under crowding stress, 2) identify relationships between phenotypic responses and gene expression patterns, and 3) identify groups of genes associated with yield and crowding stress tolerance. Under conditions of crowding stress, three high-yielding and three low-yielding sweet corn hybrids were grouped for transcriptional and phenotypic analyses. Transcriptional analyses identified from 372 to 859 common differentially expressed genes (DEGs) for each hybrid. Large gene expression pattern variation among hybrids and only 26 common DEGs across all hybrid comparisons were identified, suggesting each hybrid has a unique response to crowding stress. Over-represented biological functions of DEGs also differed among hybrids. Strong correlation was observed between: 1) modules with up-regulation in high-yielding hybrids and yield traits, and 2) modules with up-regulation in low-yielding hybrids and plant/ear traits. Modules linked with yield traits may be important crowding stress response mechanisms influencing crop yield. Functional analysis of the modules and common DEGs identified candidate crowding stress tolerant processes in photosynthesis, glycolysis, cell wall, carbohydrate/nitrogen metabolic process, chromatin, and transcription regulation. Moreover, these biological functions were greatly inter-connected, indicating the importance of improving the mechanisms as a network. PMID:26796516

  1. Yielding to Stress: Recent Developments in Viscoplastic Fluid Mechanics

    NASA Astrophysics Data System (ADS)

    Balmforth, Neil J.; Frigaard, Ian A.; Ovarlez, Guillaume

    2014-01-01

    The archetypal feature of a viscoplastic fluid is its yield stress: If the material is not sufficiently stressed, it behaves like a solid, but once the yield stress is exceeded, the material flows like a fluid. Such behavior characterizes materials common in industries such as petroleum and chemical processing, cosmetics, and food processing and in geophysical fluid dynamics. The most common idealization of a viscoplastic fluid is the Bingham model, which has been widely used to rationalize experimental data, even though it is a crude oversimplification of true rheological behavior. The popularity of the model is in its apparent simplicity. Despite this, the sudden transition between solid-like behavior and flow introduces significant complications into the dynamics, which, as a result, has resisted much analysis. Over recent decades, theoretical developments, both analytical and computational, have provided a better understanding of the effect of the yield stress. Simultaneously, greater insight into the material behavior of real fluids has been afforded by advances in rheometry. These developments have primed us for a better understanding of the various applications in the natural and engineering sciences.

  2. The rising motion of spheres in structured fluids with yield stress

    NASA Astrophysics Data System (ADS)

    Mirzaagha, S.; Pasquino, R.; Iuliano, E.; D'Avino, G.; Zonfrilli, F.; Guida, V.; Grizzuti, N.

    2017-09-01

    The rising of spherical bodies in structured fluids with yield stress is studied. The system is a suspension of hydrogenated castor oil colloidal fibers in a surfactant micellar solution. The fiber network confers to the fluid a viscoelastic behavior, with a well-defined yield stress, which increases with increasing fiber concentration. Various fluids with different fiber contents are prepared and rheologically characterized. A home-made time-lapse photography setup is used to monitor the time evolution position of the spherical particles, and the rising motion of both hollow spheres and air bubbles, in the diameter range 65-550 μm, is measured. The experiments last as long as several weeks, corresponding to significantly low measured velocities. Finite element simulations are performed to support the experimental data, assuming both interfacial slip and no slip conditions. The fluid dynamic phenomenon is studied and discussed in terms of dimensionless numbers, such as yield ratio, Bingham number, and Stokes drag coefficient. The results are novel for the system (suspending medium and hollow spheres) and for the covered Bingham number range, which is extended over three orders of magnitude in comparison with already available literature results. Our values provide quantitative data of the mechanical properties (i.e., yield stress value) at very low shear rates, in a prohibitive range for a traditional rheometer, and agree with the macroscopic rheological response. Moreover, the important role of the power law index n of the Herschel-Bulkley model, used to fit the data, has been highlighted. Our results, based on a Bingham-like fluid, are compared with the experimental data already available with Carbopol, treated as a Herschel Bulkley fluid with n = 0.5. The results could have important implications in the fabric and personal care detergency, a technological area where many fluids have composition and show rheological properties similar to those considered in the

  3. Increased yield stability of field-grown winter barley (Hordeum vulgare L.) varietal mixtures through ecological processes

    PubMed Central

    Creissen, Henry E.; Jorgensen, Tove H.; Brown, James K.M.

    2016-01-01

    Crop variety mixtures have the potential to increase yield stability in highly variable and unpredictable environments, yet knowledge of the specific mechanisms underlying enhanced yield stability has been limited. Ecological processes in genetically diverse crops were investigated by conducting field trials with winter barley varieties (Hordeum vulgare), grown as monocultures or as three-way mixtures in fungicide treated and untreated plots at three sites. Mixtures achieved yields comparable to the best performing monocultures whilst enhancing yield stability despite being subject to multiple predicted and unpredicted abiotic and biotic stresses including brown rust (Puccinia hordei) and lodging. There was compensation through competitive release because the most competitive variety overyielded in mixtures thereby compensating for less competitive varieties. Facilitation was also identified as an important ecological process within mixtures by reducing lodging. This study indicates that crop varietal mixtures have the capacity to stabilise productivity even when environmental conditions and stresses are not predicted in advance. Varietal mixtures provide a means of increasing crop genetic diversity without the need for extensive breeding efforts. They may confer enhanced resilience to environmental stresses and thus be a desirable component of future cropping systems for sustainable arable farming. PMID:27375312

  4. Suspensions of Noncolloidal Particles in Yield Stress Fluids: Experimental and Micromechanical Approaches

    NASA Astrophysics Data System (ADS)

    Mahaut, Fabien; Bertrand, François; Coussot, Philippe; Chateau, Xavier; Ovarlez, Guillaume

    2008-07-01

    We study experimentally and theoretically the behavior of suspensions of noncolloidal particles in yield stress fluids. We develop procedures and materials that allow focusing on the purely mechanical contribution of the particles to the yield stress fiuid behavior, allowing relating the macroscopic properties of these suspensions to the mechanical properties of the yield stress fluid and the particle volume fraction. We find that the elastic modulus/concentration relationship follows a Krieger-Dougherty law, and show that the yield stress/concentration relationship is related to the elastic modulus/concentration relationship through a very simple law, in agreement with a micromechanical analysis. We finally present evidence for shear-induced migration in the flows of these suspensions.

  5. Estimating yields of salt- and water-stressed forages with remote sensing in the visible and near infrared.

    PubMed

    Poss, J A; Russell, W B; Grieve, C M

    2006-01-01

    In arid irrigated regions, the proportion of crop production under deficit irrigation with poorer quality water is increasing as demand for fresh water soars and efforts to prevent saline water table development occur. Remote sensing technology to quantify salinity and water stress effects on forage yield can be an important tool to address yield loss potential when deficit irrigating with poor water quality. Two important forages, alfalfa (Medicago sativa L.) and tall wheatgrass (Agropyron elongatum L.), were grown in a volumetric lysimeter facility where rootzone salinity and water content were varied and monitored. Ground-based hyperspectral canopy reflectance in the visible and near infrared (NIR) were related to forage yields from a broad range of salinity and water stress conditions. Canopy reflectance spectra were obtained in the 350- to 1000-nm region from two viewing angles (nadir view, 45 degrees from nadir). Nadir view vegetation indices (VI) were not as strongly correlated with leaf area index changes attributed to water and salinity stress treatments for both alfalfa and wheatgrass. From a list of 71 VIs, two were selected for a multiple linear-regression model that estimated yield under varying salinity and water stress conditions. With data obtained during the second harvest of a three-harvest 100-d growing period, regression coefficients for each crop were developed and then used with the model to estimate fresh weights for preceding and succeeding harvests during the same 100-d interval. The model accounted for 72% of the variation in yields in wheatgrass and 94% in yields of alfalfa within the same salinity and water stress treatment period. The model successfully predicted yield in three out of four cases when applied to the first and third harvest yields. Correlations between indices and yield increased as canopy development progressed. Growth reductions attributed to simultaneous salinity and water stress were well characterized, but the

  6. Study of water stress effects in different growth stages on yield and yield components of different rice (Oryza sativa L.) cultivars.

    PubMed

    Sarvestani, Zinolabedin Tahmasebi; Pirdashti, Hemmatollah; Sanavy, Seyed Ali Mohammad Modarres; Balouchi, Hamidreza

    2008-05-15

    A field experiment was conducted during 2001-2003 to evaluate the effect of water stress on the yield and yield components of four rice cultivars commonly grown in Mazandaran province, Iran. In northern Iran irrigated lowland rice usually experiences water deficit during the growing season include of land preparation time, planting, tillering stage, flowering and grain filing period. Recently drought affected 20 of 28 provinces in Iran; with the southeastern, central and eastern parts of the country being most severely affected. The local and improved cultivars used were Tarom, Khazar, Fajr and Nemat. The different water stress conditions were water stress during vegetative, flowering and grain filling stages and well watered was the control. Water stress at vegetative stage significantly reduced plant height of all cultivars. Water stress at flowering stage had a greater grain yield reduction than water stress at other times. The reduction of grain yield largely resulted from the reduction in fertile panicle and filled grain percentage. Water deficit during vegetative, flowering and grain filling stages reduced mean grain yield by 21, 50 and 21% on average in comparison to control respectively. The yield advantage of two semidwarf varieties, Fajr and Nemat, were not maintained under drought stress. Total biomass, harvest index, plant height, filled grain, unfilled grain and 1000 grain weight were reduced under water stress in all cultivars. Water stress at vegetative stage effectively reduced total biomass due to decrease of photosynthesis rate and dry matter accumulation.

  7. [Effects of water stress on red-edge parameters and yield in wheat cropping].

    PubMed

    He, Ke-Xun; Zaho, Shu-He; Lai, Jian-Bin; Luo, Yun-Xiao; Qin, Zhi-Hao

    2013-08-01

    The objective of the present paper is to study the influence of water stress on wheat spectrum red edge parameters by using field wheat spectrum data obtained from water stress experiment. Firstly, the authors analyzed the influence of water stress on wheat spectrum reflectance. Then the authors got the wheat red edge position and red edge peak through calculating wheat spectrum first-order differential and analyzed the influence of water stress on wheat red edge parameters. Finally the authors discussed the relationship between red peak and wheat yield. The results showed that the wheat red edge position shows "red shift" at the beginning of the wheat growth period and "blue shift" at the later period of the wheat growth period under the water stress experiment. Also, the red edge peak of the wheat showed that red edge peak increased with the water stress sharpening at the beginning of the wheat growth period, and then the red edge peak reduced with the water stress sharpening. The wheat red edge peak presented positive correlation with the wheat yield before the elongation period, and exhibited negative correlation after that period.

  8. Yield stress in amorphous solids: A mode-coupling-theory analysis

    NASA Astrophysics Data System (ADS)

    Ikeda, Atsushi; Berthier, Ludovic

    2013-11-01

    The yield stress is a defining feature of amorphous materials which is difficult to analyze theoretically, because it stems from the strongly nonlinear response of an arrested solid to an applied deformation. Mode-coupling theory predicts the flow curves of materials undergoing a glass transition and thus offers predictions for the yield stress of amorphous solids. We use this approach to analyze several classes of disordered solids, using simple models of hard-sphere glasses, soft glasses, and metallic glasses for which the mode-coupling predictions can be directly compared to the outcome of numerical measurements. The theory correctly describes the emergence of a yield stress of entropic nature in hard-sphere glasses, and its rapid growth as density approaches random close packing at qualitative level. By contrast, the emergence of solid behavior in soft and metallic glasses, which originates from direct particle interactions is not well described by the theory. We show that similar shortcomings arise in the description of the caging dynamics of the glass phase at rest. We discuss the range of applicability of mode-coupling theory to understand the yield stress and nonlinear rheology of amorphous materials.

  9. Influence of oxidative stress and grains on sclerotial biomass and carotenoid yield of Penicillium sp. PT95.

    PubMed

    Chen, Shu-Jun; Wang, Qi; Han, Jian-Rong

    2010-08-01

    Oxidative stress and grains were evaluated for carotenoid production by solid-state fermentation using Penicillium sp. PT95. When the fungus was grown at high oxidative stress, its sclerotial biomass and carotenoid content in sclerotia increased significantly with respect to low oxidative stress (P < 0.01). High oxidative stress also caused a statistically significant increase in carotenoid yield as compared with low oxidative stress (P < 0.01). Both the sclerotial biomass and the amount of carotenoid accumulated in sclerotia of strain PT95 were strongly dependent on the grain medium used. Among the grain media tested under high oxidative stress, buckwheat medium gave the highest content of carotenoid in sclerotia (828 microg/g dry sclerotia), millet medium gave respectively the highest sclerotial biomass (12.69 g/100 g grain) and carotenoid yield (10.152 mg/100 g grain). Copyright 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

  10. Predicting the apparent viscosity and yield stress of digested and secondary sludge mixtures.

    PubMed

    Eshtiaghi, Nicky; Markis, Flora; Zain, Dwen; Mai, Kiet Hung

    2016-05-15

    The legal banning of conventional sludge disposal methods such as landfill has led to a global movement towards achieving a sustainable sludge management strategy. Reusing sludge for energy production (biogas production) through the anaerobic digestion of sludge can provide a sustainable solution. However, for the optimum performance of digesters with minimal use of energy input, operating conditions must be regulated in accordance with the rheological characteristics of the sludge. If it is assumed that only secondary sludge enters the anaerobic digesters, an impact of variations to the solids concentration and volume fraction of each sludge type must be investigated to understand how the apparent viscosity and yield stress of the secondary and digested sludge mixture inside the digesters changes. In this study, five different total solids concentration of secondary and digested sludge were mixed at different digested sludge volume fractions ranging from 0 to 1. It was found that if secondary sludge was mixed with digested sludge at the same total solids concentration, the apparent viscosity and the yield stress of the mixture increased exponentially by increasing the volume fraction of digested sludge. However, if secondary sludge was added to digested sludge with a different solids concentration, the apparent viscosity and yield stress of the resulting mixed sludge was controlled by the concentrated sludge regardless of its type. Semi - empirical correlations were proposed to predict the apparent viscosity and yield stress of the mixed digested and secondary sludge. A master curve was also developed to predict the flow behaviour of sludge mixtures regardless of the total solid concentration and volume fraction of each sludge type within the studied solids concentration range of 1.4 and 7%TS. This model can be used for digesters optimization and design by predicting the rheology of sludge mixture inside digester. Copyright © 2016 Elsevier Ltd. All rights

  11. Heat tolerance around flowering in wheat identified as a key trait for increased yield potential in Europe under climate change

    PubMed Central

    Stratonovitch, Pierre; Semenov, Mikhail A.

    2015-01-01

    To deliver food security for the 9 billon population in 2050, a 70% increase in world food supply will be required. Projected climatic and environmental changes emphasize the need for breeding strategies that delivers both a substantial increase in yield potential and resilience to extreme weather events such as heat waves, late frost, and drought. Heat stress around sensitive stages of wheat development has been identified as a possible threat to wheat production in Europe. However, no estimates have been made to assess yield losses due to increased frequency and magnitude of heat stress under climate change. Using existing experimental data, the Sirius wheat model was refined by incorporating the effects of extreme temperature during flowering and grain filling on accelerated leaf senescence, grain number, and grain weight. This allowed us, for the first time, to quantify yield losses resulting from heat stress under climate change. The model was used to optimize wheat ideotypes for CMIP5-based climate scenarios for 2050 at six sites in Europe with diverse climates. The yield potential for heat-tolerant ideotypes can be substantially increased in the future (e.g. by 80% at Seville, 100% at Debrecen) compared with the current cultivars by selecting an optimal combination of wheat traits, e.g. optimal phenology and extended duration of grain filling. However, at two sites, Seville and Debrecen, the grain yields of heat-sensitive ideotypes were substantially lower (by 54% and 16%) and more variable compared with heat-tolerant ideotypes, because the extended grain filling required for the increased yield potential was in conflict with episodes of high temperature during flowering and grain filling. Despite much earlier flowering at these sites, the risk of heat stress affecting yields of heat-sensitive ideotypes remained high. Therefore, heat tolerance in wheat is likely to become a key trait for increased yield potential and yield stability in southern Europe in the

  12. When sticky fluids don't stick: yield-stress fluid drops on heated surfaces

    NASA Astrophysics Data System (ADS)

    Blackwell, Brendan; Wu, Alex; Ewoldt, Randy

    2016-11-01

    Yield-stress fluids, including gels and pastes, are effectively fluid at high stress and solid at low stress. In liquid-solid impacts, these fluids can stick and accumulate where they impact; this sticky behavior motivates several applications of these rheologically-complex materials. Here we describe experiments with aqueous yield stress fluids that are more 'sticky' than water at room temperature (e.g. supporting larger coating thicknesses), but are less 'sticky' at higher temperatures. Specifically, we study the conditions for aqueous yield stress fluids to bounce and slide on heated surfaces when water sticks. Here we present high-speed imaging and color interferometry to observe the thickness of the vapor layer between the drop and the surface during both stick and non-stick events. We use these data to gain insight into the physics behind the phenomenon of the yield-stress fluids bouncing and sliding, rather than sticking, on hot surfaces.

  13. Drought-Tolerant Corn Hybrids Yield More in Drought-Stressed Environments with No Penalty in Non-stressed Environments

    PubMed Central

    Adee, Eric; Roozeboom, Kraig; Balboa, Guillermo R.; Schlegel, Alan; Ciampitti, Ignacio A.

    2016-01-01

    The potential benefit of drought-tolerant (DT) corn (Zea mays L.) hybrids may depend on drought intensity, duration, crop growth stage (timing), and the array of drought tolerance mechanisms present in selected hybrids. We hypothesized that corn hybrids containing DT traits would produce more consistent yields compared to non-DT hybrids in the presence of drought stress. The objective of this study was to define types of production environments where DT hybrids have a yield advantage compared to non-DT hybrids. Drought tolerant and non-DT hybrid pairs of similar maturity were planted in six site-years with different soil types, seasonal evapotranspiration (ET), and vapor pressure deficit (VPD), representing a range of macro-environments. Irrigation regimes and seeding rates were used to create several micro-environments within each macro-environment. Hybrid response to the range of macro and micro-environmental stresses were characterized in terms of water use efficiency, grain yield, and environmental index. Yield advantage of DT hybrids was positively correlated with environment ET and VPD. Drought tolerant hybrids yielded 5 to 7% more than non-DT hybrids in high and medium ET environments (>430 mm ET), corresponding to seasonal VPD greater than 1200 Pa. Environmental index analysis confirmed that DT hybrids were superior in stressful environments. Yield advantage for DT hybrids appeared as yield dropped below 10.8 Mg ha-1 and averaged as much as 0.6–1 Mg ha-1 at the low yield range. Hybrids with DT technology can offer a degree of buffering against drought stress by minimizing yield reduction, but also maintaining a comparable yield potential in high yielding environments. Further studies should focus on the physiological mechanisms presented in the commercially available corn drought tolerant hybrids. PMID:27790237

  14. Predicting the apparent viscosity and yield stress of mixtures of primary, secondary and anaerobically digested sewage sludge: Simulating anaerobic digesters.

    PubMed

    Markis, Flora; Baudez, Jean-Christophe; Parthasarathy, Rajarathinam; Slatter, Paul; Eshtiaghi, Nicky

    2016-09-01

    Predicting the flow behaviour, most notably, the apparent viscosity and yield stress of sludge mixtures inside the anaerobic digester is essential because it helps optimize the mixing system in digesters. This paper investigates the rheology of sludge mixtures as a function of digested sludge volume fraction. Sludge mixtures exhibited non-Newtonian, shear thinning, yield stress behaviour. The apparent viscosity and yield stress of sludge mixtures prepared at the same total solids concentration was influenced by the interactions within the digested sludge and increased with the volume fraction of digested sludge - highlighted using shear compliance and shear modulus of sludge mixtures. However, when a thickened primary - secondary sludge mixture was mixed with dilute digested sludge, the apparent viscosity and yield stress decreased with increasing the volume fraction of digested sludge. This was caused by the dilution effect leading to a reduction in the hydrodynamic and non-hydrodynamic interactions when dilute digested sludge was added. Correlations were developed to predict the apparent viscosity and yield stress of the mixtures as a function of the digested sludge volume fraction and total solids concentration of the mixtures. The parameters of correlations can be estimated using pH of sludge. The shear and complex modulus were also modelled and they followed an exponential relationship with increasing digested sludge volume fraction. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. Hydrogen bonding and interparticle forces in platelet alpha-Al2O3 dispersions: yield stress and zeta potential.

    PubMed

    Khoo, Kay-Sen; Teh, E-Jen; Leong, Yee-Kwong; Ong, Ban Choon

    2009-04-09

    Adsorbed phosphate on smooth platelet alpha-Al2O3 particles at saturation surface coverage gives rise to strong interparticle attractive forces in dispersion. The maximum yield stress at the point of zero charge was increased by 2-fold. This was attributed to a high density of intermolecular hydrogen bonding between the adsorbed phosphate layers of the interacting particles. Adsorbed citrate at saturation surface coverage, however, reduced the maximum yield stress by 50%. It adsorbed to form a very effective steric barrier as intramolecular hydrogen bonding between -OH and the free terminal carboxylic group prevented strong interactions with other adsorbed citrate molecules residing on the second interacting particle. This steric barrier kept the interacting platelet particles further apart, thereby weakening the van der Waals attraction. The platelet alpha-Al2O3 dispersions were flocculated at all pH level. These dispersions displayed a maximum yield stress at the point of zero zeta potential at the pH approximately 8.0. They also obeyed the yield stress-DLVO force model as characterized by a linear decrease in the yield stress with the square of the zeta potential.

  16. Challenges in breeding for yield increase for drought.

    PubMed

    Sinclair, Thomas R

    2011-06-01

    Crop genetic improvement for environmental stress at the molecular and physiological level is very complex and challenging. Unlike the example of the current major commercial transgenic crops for which biotic stress tolerance is based on chemicals alien to plants, the complex, redundant and homeostatic molecular and physiological systems existing in plants must be altered for drought tolerance improvement. Sophisticated tools must be developed to monitor phenotype expression at the crop level to characterize variation among genotypes across a range of environments. Once stress-tolerant cultivars are developed, regional probability distributions describing yield response across years will be necessary. This information can then aid in identifying environmental conditions for positive and negative responses to genetic modification to guide farmer selection of stress-tolerant cultivars. Copyright © 2011 Elsevier Ltd. All rights reserved.

  17. Chemical intervention in plant sugar signalling increases yield and resilience

    NASA Astrophysics Data System (ADS)

    Griffiths, Cara A.; Sagar, Ram; Geng, Yiqun; Primavesi, Lucia F.; Patel, Mitul K.; Passarelli, Melissa K.; Gilmore, Ian S.; Steven, Rory T.; Bunch, Josephine; Paul, Matthew J.; Davis, Benjamin G.

    2016-12-01

    The pressing global issue of food insecurity due to population growth, diminishing land and variable climate can only be addressed in agriculture by improving both maximum crop yield potential and resilience. Genetic modification is one potential solution, but has yet to achieve worldwide acceptance, particularly for crops such as wheat. Trehalose-6-phosphate (T6P), a central sugar signal in plants, regulates sucrose use and allocation, underpinning crop growth and development. Here we show that application of a chemical intervention strategy directly modulates T6P levels in planta. Plant-permeable analogues of T6P were designed and constructed based on a ‘signalling-precursor’ concept for permeability, ready uptake and sunlight-triggered release of T6P in planta. We show that chemical intervention in a potent sugar signal increases grain yield, whereas application to vegetative tissue improves recovery and resurrection from drought. This technology offers a means to combine increases in yield with crop stress resilience. Given the generality of the T6P pathway in plants and other small-molecule signals in biology, these studies suggest that suitable synthetic exogenous small-molecule signal precursors can be used to directly enhance plant performance and perhaps other organism function.

  18. Do maize models capture the impacts of heat and drought stresses on yield? Using algorithm ensembles to identify successful approaches.

    PubMed

    Jin, Zhenong; Zhuang, Qianlai; Tan, Zeli; Dukes, Jeffrey S; Zheng, Bangyou; Melillo, Jerry M

    2016-09-01

    Stresses from heat and drought are expected to increasingly suppress crop yields, but the degree to which current models can represent these effects is uncertain. Here we evaluate the algorithms that determine impacts of heat and drought stress on maize in 16 major maize models by incorporating these algorithms into a standard model, the Agricultural Production Systems sIMulator (APSIM), and running an ensemble of simulations. Although both daily mean temperature and daylight temperature are common choice of forcing heat stress algorithms, current parameterizations in most models favor the use of daylight temperature even though the algorithm was designed for daily mean temperature. Different drought algorithms (i.e., a function of soil water content, of soil water supply to demand ratio, and of actual to potential transpiration ratio) simulated considerably different patterns of water shortage over the growing season, but nonetheless predicted similar decreases in annual yield. Using the selected combination of algorithms, our simulations show that maize yield reduction was more sensitive to drought stress than to heat stress for the US Midwest since the 1980s, and this pattern will continue under future scenarios; the influence of excessive heat will become increasingly prominent by the late 21st century. Our review of algorithms in 16 crop models suggests that the impacts of heat and drought stress on plant yield can be best described by crop models that: (i) incorporate event-based descriptions of heat and drought stress, (ii) consider the effects of nighttime warming, and (iii) coordinate the interactions among multiple stresses. Our study identifies the proficiency with which different model formulations capture the impacts of heat and drought stress on maize biomass and yield production. The framework presented here can be applied to other modeled processes and used to improve yield predictions of other crops with a wide variety of crop models. © 2016 John

  19. Effect of the Yield Stress and r-value Distribution on the Earing Profile of Cup Drawing with Yld2000-2d Yield Function

    NASA Astrophysics Data System (ADS)

    Lou, Yanshan; Bae, Gihyun; Lee, Changsoo; Huh, Hoon

    2010-06-01

    This paper deals with the effect of the yield stress and r-value distribution on the earing in the cup drawing. The anisotropic yield function, Yld2000-2d yield function, is selected to describe the anisotropy of two metal sheets, 719B and AA5182-O. The tool dimension is referred from the Benchmark problem of NUMISHEET'2002. The Downhill Simplex method is applied to identify the anisotropic coefficients in Yld2000-2d yield function. Simulations of the drawing process are performed to investigate the earing profile of two materials. The earing profiles obtained from simulations are compared with the analytical model developed by Hosford and Caddell. Simulations are conducted with respect to the change of the yield stress and r-value distribution, respectively. The correlation between the anisotropy and the earing tendency is investigated based on simulation data. Finally, the earing mechanism is analyzed through the deformation process of the blank during the cup deep drawing. It can be concluded that ears locate at angular positions with lower yield stress and higher r-value while the valleys appear at the angular position with higher yield stress and lower r-value. The effect of the yield stress distribution is more important for the cup height distribution than that of the r-value distribution.

  20. Giant intracranial aneurysm embolization with a yield stress fluid material: insights from CFD analysis.

    PubMed

    Wang, Weixiong; Graziano, Francesca; Russo, Vittorio; Ulm, Arthur J; De Kee, Daniel; Khismatullin, Damir B

    2013-01-01

    The endovascular treatment of intracranial aneurysms remains a challenge, especially when the aneurysm is large in size and has irregular, non-spherical geometry. In this paper, we use computational fluid dynamics to simulate blood flow in a vertebro-basilar junction giant aneurysm for the following three cases: (1) an empty aneurysm, (2) an aneurysm filled with platinum coils, and (3) an aneurysm filled with a yield stress fluid material. In the computational model, blood and the coil-filled region are treated as a non-Newtonian fluid and an isotropic porous medium, respectively. The results show that yield stress fluids can be used for aneurysm embolization provided the yield stress value is 20 Pa or higher. Specifically, flow recirculation in the aneurysm and the size of the inflow jet impingement zone on the aneurysm wall are substantially reduced by yield stress fluid treatment. Overall, this study opens up the possibility of using yield stress fluids for effective embolization of large-volume intracranial aneurysms.

  1. Dynamics of yield-stress droplets: Morphology of impact craters

    NASA Astrophysics Data System (ADS)

    Neufeld, Jerome; Sohr, David; Ferrari, Leo; Dalziel, Stuart

    2017-11-01

    Yield strength can play an important role for the dynamics of droplets impacting on surfaces, whether at the industrial or planetary scale, and can capture a zoo of impact crater morphologies, from simple parabolic craters, to more complex forms with forms with, for example, multiple rings, central peaks. Here we show that the morphology of planetary impact craters can be reproduced in the laboratory using carbopol, a transparent yield-stress fluid, as both impactor and bulk fluid. Using high-speed video photography, we characterise the universal, transient initial excavation stage of impact and show the dependence of the subsequent relaxation to final crater morphology on impactor size, impact speed and yield stress. To further interrogate our laboratory impacts, we dye our impactor to map its final distribution and use particle tracking to determine the flow fields during impact and the maximal extent of the yield surface. We characterise the flow-fields induced during impact, and the maximal extent of the yield surface, by tracking particles within the bulk fluid and map the distribution of impactor and bulk by tracing the final distribution of dyed impactor. The results of laboratory impact droplets are used to infer the properties of planetary impactors, and aid in inter.

  2. Potassium Management for Improving Growth and Grain Yield of Maize (Zea mays L.) under Moisture Stress Condition

    PubMed Central

    Amanullah; Iqbal, Asif; Irfanullah; Hidayat, Zeeshan

    2016-01-01

    Potassium (K) fertilizer management is beneficial for improving growth, yield and yield components of field crops under moisture stress condition in semiarid climates. Field experiments were conducted to study the response of maize (Zea mays L., cv. Azam) to foliar and soil applied K during summer 2013 and 2014. The experiments were carried out at the Agronomy Research Farm of The University of Agriculture Peshawar, Northwest Pakistan under limited irrigation (moisture stress) condition. It was concluded from the results that application of foliar K at the rate of 1–3% and foliar Zn at the rate of 0.1–0.2% was more beneficial in terms of better growth, higher yield and yield components of maize under moisture stress condition. Early spray (vegetative stage) resulted in better growth and higher yield than late spray (reproductive stage). Soil K treated plots (rest) plots performed better than control (K not applied) in terms of improved growth, higher yield and yield components of maize crop. The results further demonstrated that increasing the rate of soil applied K up to 90 kg P ha−1 in two equal splits (50% each at sowing and knee height) improve growth and maize productivity under semiarid climates. PMID:27694964

  3. Potassium Management for Improving Growth and Grain Yield of Maize (Zea mays L.) under Moisture Stress Condition.

    PubMed

    Amanullah; Iqbal, Asif; Irfanullah; Hidayat, Zeeshan

    2016-10-03

    Potassium (K) fertilizer management is beneficial for improving growth, yield and yield components of field crops under moisture stress condition in semiarid climates. Field experiments were conducted to study the response of maize (Zea mays L., cv. Azam) to foliar and soil applied K during summer 2013 and 2014. The experiments were carried out at the Agronomy Research Farm of The University of Agriculture Peshawar, Northwest Pakistan under limited irrigation (moisture stress) condition. It was concluded from the results that application of foliar K at the rate of 1-3% and foliar Zn at the rate of 0.1-0.2% was more beneficial in terms of better growth, higher yield and yield components of maize under moisture stress condition. Early spray (vegetative stage) resulted in better growth and higher yield than late spray (reproductive stage). Soil K treated plots (rest) plots performed better than control (K not applied) in terms of improved growth, higher yield and yield components of maize crop. The results further demonstrated that increasing the rate of soil applied K up to 90 kg P ha -1 in two equal splits (50% each at sowing and knee height) improve growth and maize productivity under semiarid climates.

  4. Crop Diversity for Yield Increase

    PubMed Central

    Li, Chengyun; He, Xiahong; Zhu, Shusheng; Zhou, Huiping; Wang, Yunyue; Li, Yan; Yang, Jing; Fan, Jinxiang; Yang, Jincheng; Wang, Guibin; Long, Yunfu; Xu, Jiayou; Tang, Yongsheng; Zhao, Gaohui; Yang, Jianrong; Liu, Lin; Sun, Yan; Xie, Yong; Wang, Haining; Zhu, Youyong

    2009-01-01

    Traditional farming practices suggest that cultivation of a mixture of crop species in the same field through temporal and spatial management may be advantageous in boosting yields and preventing disease, but evidence from large-scale field testing is limited. Increasing crop diversity through intercropping addresses the problem of increasing land utilization and crop productivity. In collaboration with farmers and extension personnel, we tested intercropping of tobacco, maize, sugarcane, potato, wheat and broad bean – either by relay cropping or by mixing crop species based on differences in their heights, and practiced these patterns on 15,302 hectares in ten counties in Yunnan Province, China. The results of observation plots within these areas showed that some combinations increased crop yields for the same season between 33.2 and 84.7% and reached a land equivalent ratio (LER) of between 1.31 and 1.84. This approach can be easily applied in developing countries, which is crucial in face of dwindling arable land and increasing food demand. PMID:19956624

  5. [Effects of postponing nitrogen application on photosynthetic characteristics and grain yield of winter wheat subjected to water stress after heading stage].

    PubMed

    Yang, Ming-da; Ma, Shou-chen; Yang, Shen-jiao; Zhang, Su-yu; Guan, Xiao-kang; Li, Xue-mei; Wang, Tong-chao; Li, Chun-xi

    2015-11-01

    A pot culture experiment was conducted to study the effects of postponing nitrogen (N) application on photosynthetic characteristics and grain yield of winter wheat subjected to water stress after heading stage. Equal in the total N rate in winter wheat growth season, N application was split before sowing, and/or at jointing and /or at anthesis at the ratio of 10:0:0 (N1), 6:4:0 (N2) and 4:3:3 (N3), combined with unfavorable water condition (either waterlogged or drought) with the sufficient water condition as control. The results showed that, under each of the water condition, both N2 and N3 treatments significantly improved the leaf photosynthetic rate and the SPAD value of flag leaf compared with N1 treatment during grain filling stage, and also the crop ear number, grain number per spike and above-ground biomass were increased. Although postponing nitrogen application increased water consumption, both grain yield and water use efficiency were increased. Compared with sufficient water supply, drought stress and waterlogging stress significantly reduced the photosynthetic rate of flag leaves at anthesis and grain filling stages, ear number, 1000-grain mass and yield under all of the N application patterns. The decline of photosynthetic rate under either drought stress or waterlogging stress was much less in N2 and N3 than in N1 treatments, just the same as the grain yield. The results indicated that postponing nitrogen application could regulate winter wheat yield as well as its components to alleviate the damages, caused by unfavorable water stress by increasing flag leaf SPAD and maintaining flag leaf photosynthetic rate after anthesis, and promoting above-ground dry matter accumulation.

  6. Asymmetric Yield Function Based on the Stress Invariants for Pressure Sensitive Metals

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jeong Wahn Yoon; Yanshan Lou; Jong Hun Yoon

    A general asymmetric yield function is proposed with dependence on the stress invariants for pressure sensitive metals. The pressure sensitivity of the proposed yield function is consistent with the experimental result of Spitzig and Richmond (1984) for steel and aluminum alloys while the asymmetry of the third invariant is preserved to model strength differential (SD) effect of pressure insensitive materials. The proposed yield function is transformed in the space of the stress triaxaility, the von Mises stress and the normalized invariant to theoretically investigate the possible reason of the SD effect. The proposed plasticity model is further extended to characterizemore » the anisotropic behavior of metals both in tension and compression. The extension of the yield function is realized by introducing two distinct fourth-order linear transformation tensors of the stress tensor for the second and third invariants, respectively. The extended yield function reasonably models the evolution of yield surfaces for a zirconium clock-rolled plate during in-plane and through-thickness compression reported by Plunkett et al. (2007). The extended yield function is also applied to describe the orthotropic behavior of a face-centered cubic metal of AA 2008-T4 and two hexagonal close-packed metals of high-purity-titanium and AZ31 magnesium alloy. The orthotropic behavior predicted by the generalized model is compared with experimental results of these metals. The comparison validates that the proposed yield function provides sufficient predictability on SD effect and anisotropic behavior both in tension and compression. When it is necessary to consider r-value anisotropy, the proposed function is efficient to be used with nonassociated flow plasticity by introducing a separate plastic potential for the consideration of r-values as shown in Stoughton & Yoon (2004, 2009).« less

  7. Knockdown of an inflorescence meristem-specific cytokinin oxidase - OsCKX2 in rice reduces yield penalty under salinity stress condition.

    PubMed

    Joshi, Rohit; Sahoo, Khirod Kumar; Tripathi, Amit Kumar; Kumar, Ritesh; Gupta, Brijesh Kumar; Pareek, Ashwani; Singla-Pareek, Sneh Lata

    2018-05-01

    Cytokinins play a significant role in determining grain yield in plants. Cytokinin oxidases catalyse irreversible degradation of cytokinins and hence modulate cellular cytokinin levels. Here, we studied the role of an inflorescence meristem-specific rice cytokinin oxidase - OsCKX2 - in reducing yield penalty under salinity stress conditions. We utilized an RNAi-based approach to study the function of OsCKX2 in maintaining grain yield under salinity stress condition. Ultra-performance liquid chromatography-based estimation revealed a significant increase in cytokinins in the inflorescence meristem of OsCKX2-knockdown plants. To determine if there exists a correlation between OsCKX2 levels and yield under salinity stress condition, we assessed the growth, physiology and grain yield of OsCKX2-knockdown plants vis-à-vis the wild type. OsCKX2-knockdown plants showed better vegetative growth, higher relative water content and photosynthetic efficiency and reduced electrolyte leakage as compared with the wild type under salinity stress. Importantly, we found a negative correlation between OsCKX2 expression and plant productivity as evident by assessment of agronomical parameters such as panicle branching, filled grains per plant and harvest index both under control and salinity stress conditions. These results suggest that OsCKX2, via controlling cytokinin levels, regulates floral primordial activity modulating rice grain yield under normal as well as abiotic stress conditions. © 2017 John Wiley & Sons Ltd.

  8. Phenotypic Stability of Zea mays Grain Yield and Its Attributing Traits under Drought Stress

    PubMed Central

    Ali, Fawad; Ahsan, Muhammad; Ali, Qurban; Kanwal, Naila

    2017-01-01

    Phenotypic stability under stress environment facilitate the fitness of genotype and opens new horizons to explore the cryptic genetic variation. Variation in tolerance to drought stress, a major grain yield constraint to global maize production, was identified, at the phenotypic and genotypic level. Here we found a prominent hybrid H9 that showed fitness over four growing seasons for grain yield under water stress conditions. Genotypic and phenotypic correlation of yield attributing traits over four seasons demonstrated that cobs per plant, 100 seed weight, number of grains rows per cob, total dry matter, cob diameter had positive association (r2 = 0.3–0.9) to grain yield. The perturbation was found for chlorophyll content as it showed moderate to strong association (P < 0.01) over four seasons, might be due to environment or genotype dependent. Highest heritability (95%) and genetic advance (79%) for grain yield was found in H9 over four consecutive crop growing seasons. Combined analysis over four seasons showed that studied variables together explained 85% of total variation in dependent structure (grain yield) obtained by Principal component analysis. This significant finding is the best example of phenotypic stability of grain yield in H9 and made it best fitted for grain yield under drought stress scenario. Detailed genetic analysis of H9 will help us to identify significant loci and alleles that made H9 the best fitted and it could serve as a potential source to generate novel transgressive levels of tolerance for drought stress in arid/semiarid regions. PMID:28878785

  9. Phenotypic Stability of Zea mays Grain Yield and Its Attributing Traits under Drought Stress.

    PubMed

    Ali, Fawad; Ahsan, Muhammad; Ali, Qurban; Kanwal, Naila

    2017-01-01

    Phenotypic stability under stress environment facilitate the fitness of genotype and opens new horizons to explore the cryptic genetic variation. Variation in tolerance to drought stress, a major grain yield constraint to global maize production, was identified, at the phenotypic and genotypic level. Here we found a prominent hybrid H 9 that showed fitness over four growing seasons for grain yield under water stress conditions. Genotypic and phenotypic correlation of yield attributing traits over four seasons demonstrated that cobs per plant, 100 seed weight, number of grains rows per cob, total dry matter, cob diameter had positive association ( r 2 = 0.3-0.9) to grain yield. The perturbation was found for chlorophyll content as it showed moderate to strong association ( P < 0.01) over four seasons, might be due to environment or genotype dependent. Highest heritability (95%) and genetic advance (79%) for grain yield was found in H 9 over four consecutive crop growing seasons. Combined analysis over four seasons showed that studied variables together explained 85% of total variation in dependent structure (grain yield) obtained by Principal component analysis. This significant finding is the best example of phenotypic stability of grain yield in H 9 and made it best fitted for grain yield under drought stress scenario. Detailed genetic analysis of H 9 will help us to identify significant loci and alleles that made H 9 the best fitted and it could serve as a potential source to generate novel transgressive levels of tolerance for drought stress in arid/semiarid regions.

  10. Effect of Simultaneous Water Deficit Stress and Meloidogyne incognita Infection on Cotton Yield and Fiber Quality

    PubMed Central

    Davis, R. F.; Earl, H. J.; Timper, P.

    2014-01-01

    Both water deficit stress and Meloidogyne incognita infection can reduce cotton growth and yield, and drought can affect fiber quality, but the effect of nematodes on fiber quality is not well documented. To determine whether nematode parasitism affects fiber quality and whether the combined effects of nematode and drought stress on yield and quality are additive (independent effects), synergistic, or antagonistic, we conducted a study for 7 yr in a field infested with M. incognita. A split-plot design was used with the main plot factor as one of three irrigation treatments (low [nonirrigated], moderate irrigation, and high irrigation [water-replete]) and the subplot factor as 0 or 56 l/ha 1,3-dichloropropene. We prevented water deficit stress in plots designated as water-replete by supplementing rainfall with irrigation. Plots receiving moderate irrigation received half the water applied to the water-replete treatment. The severity of root galling was greater in nonfumigated plots and in plots receiving the least irrigation, but the amount of irrigation did not influence the effect of fumigation on root galling (no irrigation × fumigation interaction). The weights of lint and seed harvested were reduced in nonfumigated plots and also decreased as the level of irrigation decreased, but fumigation did not influence the effect of irrigation. Nematodes affected fiber quality by increasing micronaire readings but typically had little or no effect on percent lint, fiber length (measured by HVI), uniformity, strength, elongation, length (based on weight or number measured by AFIS), upper quartile length, or short fiber content (based on weight or number). Micronaire also was increased by water deficit stress, but the effects from nematodes and water stress were independent. We conclude that the detrimental effects caused to cotton yield and quality by nematode parasitism and water deficit stress are independent and therefore additive. PMID:24987162

  11. Yield-stress fluids foams: flow patterns and controlled production in T-junction and flow-focusing devices.

    PubMed

    Laborie, Benoit; Rouyer, Florence; Angelescu, Dan E; Lorenceau, Elise

    2016-11-23

    We study the formation of yield-stress fluid foams in millifluidic flow-focusing and T-junction devices. First, we provide a phase diagram for the unsteady operating regimes of bubble production when the gas pressure and the yield-stress fluid flow rate are imposed. Three regimes are identified: a co-flow of gas and yield-stress fluid, a transient production of bubble and a flow of yield-stress fluid only. Taking wall slip into account, we provide a model for the pressure at the onset of bubble formation. Then, we detail and compare two simple methods to ensure steady bubble production: regulation of the gas pressure or flow-rate. These techniques, which are easy to implement, thus open pathways for controlled production of dry yield-stress fluid foams as shown at the end of this article.

  12. Growth, physiology and yield of durum wheat (Triticum durum) treated with sewage sludge under water stress conditions

    PubMed Central

    Boudjabi, Sonia; Kribaa, Mohammed; Chenchouni, Haroun

    2015-01-01

    In arid and semi-arid areas, low soil fertility and water deficit considerably limit crop production. The use of sewage sludge as an organic amendment could contribute to the improvement of soil fertility and hence the agronomic production. The study aims to highlight the behaviour of durum wheat to the application of sewage sludge associated with water stress. The assessment focused on morphophysiological parameters of the wheat plant and yield. Under greenhouse conditions, the variety Mohamed Ben Bachir was treated by four water stress levels (100 %, 80 %, 50 % and 30 %). Each stress level comprised five fertilizer treatments: 20, 50 and 100 t/ha of dry sludge, 35 kg/ha of urea, and a control with no fertilization. Results revealed a significant loss in water content and chlorophyll a in leaves. Water stress negatively affected the development of wheat plants by reducing significantly seed yield, leaf area and biomass produced. Plant’s responses to water stress manifested by an accumulation of proline and a decrease in total phosphorus. However, the increasing doses of sewage sludge limited the effect of water stress. Our findings showed an increase in the amount of chlorophyll pigments, leaf area, total phosphorus, biomass and yield. In addition, excessive accumulation of proline (1.11 ± 1.03 µg/g DM) was recorded as a result of the high concentration of sludge (100 t/ha DM). The application of sewage sludge is beneficial for the wheat crop, but the high accumulation of proline in plants treated with high dose of sludge suggests to properly consider this fact. The application of sludge should be used with caution in soils where water is limited. Because the combined effect of these two factors could result in a fatal osmotic stress to crop development. PMID:26417365

  13. Expression of the Arabidopsis thaliana BBX32 gene in soybean increases grain yield.

    PubMed

    Preuss, Sasha B; Meister, Robert; Xu, Qingzhang; Urwin, Carl P; Tripodi, Federico A; Screen, Steven E; Anil, Veena S; Zhu, Shuquan; Morrell, James A; Liu, Grace; Ratcliffe, Oliver J; Reuber, T Lynne; Khanna, Rajnish; Goldman, Barry S; Bell, Erin; Ziegler, Todd E; McClerren, Amanda L; Ruff, Thomas G; Petracek, Marie E

    2012-01-01

    Crop yield is a highly complex quantitative trait. Historically, successful breeding for improved grain yield has led to crop plants with improved source capacity, altered plant architecture, and increased resistance to abiotic and biotic stresses. To date, transgenic approaches towards improving crop grain yield have primarily focused on protecting plants from herbicide, insects, or disease. In contrast, we have focused on identifying genes that, when expressed in soybean, improve the intrinsic ability of the plant to yield more. Through the large scale screening of candidate genes in transgenic soybean, we identified an Arabidopsis thaliana B-box domain gene (AtBBX32) that significantly increases soybean grain yield year after year in multiple transgenic events in multi-location field trials. In order to understand the underlying physiological changes that are associated with increased yield in transgenic soybean, we examined phenotypic differences in two AtBBX32-expressing lines and found increases in plant height and node, flower, pod, and seed number. We propose that these phenotypic changes are likely the result of changes in the timing of reproductive development in transgenic soybean that lead to the increased duration of the pod and seed development period. Consistent with the role of BBX32 in A. thaliana in regulating light signaling, we show that the constitutive expression of AtBBX32 in soybean alters the abundance of a subset of gene transcripts in the early morning hours. In particular, AtBBX32 alters transcript levels of the soybean clock genes GmTOC1 and LHY-CCA1-like2 (GmLCL2). We propose that through the expression of AtBBX32 and modulation of the abundance of circadian clock genes during the transition from dark to light, the timing of critical phases of reproductive development are altered. These findings demonstrate a specific role for AtBBX32 in modulating soybean development, and demonstrate the validity of expressing single genes in crops to

  14. Effect of different N fertilizer forms on antioxidant capacity and grain yield of rice growing under Cd stress.

    PubMed

    Jalloh, Mohamed Alpha; Chen, Jinghong; Zhen, Fanrong; Zhang, Guoping

    2009-03-15

    Cadmium contamination in soil has become a serious issue in sustainable agriculture production and food safety. A pot experiment was conducted to study the influence of four N fertilizer forms on grain yield, Cd concentration in plant tissues and oxidative stress under two Cd levels (0 and 100 mg Cd kg(-1)soil). The results showed that both N form and Cd stress affected grain yield, with urea-N and NH(4)(+)-N treatments having significantly higher grain yields, and Cd addition reducing yield. NO(3)(-)-N and NH(4)(+)-N treated plants had the highest and lowest Cd concentration in plant tissues, respectively. Urea-N and NH(4)(+)-N treatments had significantly higher N accumulation in plant tissues than other two N treatments. Cd addition caused a significant increase in leaf superoxide dismutase (SOD) and peroxidase (POD) activities for all N treatments, except for NO(3)(-)-N treatment, with urea-N and NH(4)(+)-N treated plants having more increase than organic-N treated ones. The results indicated that growth inhibition, yield reduction and Cd uptake of rice plants in response to Cd addition varied with the N fertilizer form.

  15. Kinematics of spheres moving through yield-stress fluids

    NASA Astrophysics Data System (ADS)

    Habdas, Piotr; de Bruyn, John R.

    2001-11-01

    When an object moves in a material with a yield stress τ_c, the material near the object generally experiences stresses higher than τc and so is fluid. Farther from the object the local stress is less than τc and so the material there is effectively solid. We have studied the motion of metal spheres being pulled through colloidal suspensions by a constant applied force in an Atwood's machine. By measuring the drag force on the sphere as a function of container size we can determine the extent of the fluidized region surrounding the sphere. We find that the drag force is not proportional to the velocity, as it is for Newtonian fluids, and so the form of the spheres' acceleration provides information about the rheology of the suspensions.

  16. Boosting autofermentation rates and product yields with sodium stress cycling: application to production of renewable fuels by cyanobacteria.

    PubMed

    Carrieri, Damian; Momot, Dariya; Brasg, Ian A; Ananyev, Gennady; Lenz, Oliver; Bryant, Donald A; Dismukes, G Charles

    2010-10-01

    Sodium concentration cycling was examined as a new strategy for redistributing carbon storage products and increasing autofermentative product yields following photosynthetic carbon fixation in the cyanobacterium Arthrospira (Spirulina) maxima. The salt-tolerant hypercarbonate strain CS-328 was grown in a medium containing 0.24 to 1.24 M sodium, resulting in increased biosynthesis of soluble carbohydrates to up to 50% of the dry weight at 1.24 M sodium. Hypoionic stress during dark anaerobic metabolism (autofermentation) was induced by resuspending filaments in low-sodium (bi)carbonate buffer (0.21 M), which resulted in accelerated autofermentation rates. For cells grown in 1.24 M NaCl, the fermentative yields of acetate, ethanol, and formate increase substantially to 1.56, 0.75, and 1.54 mmol/(g [dry weight] of cells·day), respectively (36-, 121-, and 6-fold increases in rates relative to cells grown in 0.24 M NaCl). Catabolism of endogenous carbohydrate increased by approximately 2-fold upon hypoionic stress. For cultures grown at all salt concentrations, hydrogen was produced, but its yield did not correlate with increased catabolism of soluble carbohydrates. Instead, ethanol excretion becomes a preferred route for fermentative NADH reoxidation, together with intracellular accumulation of reduced products of acetyl coenzyme A (acetyl-CoA) formation when cells are hypoionically stressed. In the absence of hypoionic stress, hydrogen production is a major beneficial pathway for NAD(+) regeneration without wasting carbon intermediates such as ethanol derived from acetyl-CoA. This switch presumably improves the overall cellular economy by retaining carbon within the cell until aerobic conditions return and the acetyl unit can be used for biosynthesis or oxidized via respiration for a much greater energy return.

  17. Experimental determination of the yield stress curve of the scotch pine wood materials

    NASA Astrophysics Data System (ADS)

    Günay, Ezgi; Aygün, Cevdet; Kaya, Şükrü Tayfun

    2013-12-01

    Yield stress curve is determined for the pine wood specimens by conducting a series of tests. In this work, pinewood is modeled as a composite material with transversely isotropic fibers. Annual rings (wood grain) of the wood specimens are taken as the major fiber directions with which the strain gauge directions are aligned. For this purpose, three types of tests are arranged. These are tensile, compression and torsion loading tests. All of the tests are categorized with respect to fiber orientations and their corresponding loading conditions. Each test within these categories is conducted separately. Tensile and compression tests are conducted in accordance with standards of Turkish Standards Institution (TSE) whereas torsion tests are conducted in accordance with Standards Australia. Specimens are machined from woods of Scotch pine which is widely used in boat building industries and in other structural engineering applications. It is determined that this species behaves more flexibly than the others. Strain gauges are installed on the specimen surfaces in such a way that loading measurements are performed along directions either parallel or perpendicular to the fiber directions. During the test and analysis phase of yield stress curve, orientation of strain gauge directions with respect to fiber directions are taken into account. The diagrams of the normal stress vs. normal strain or the shear stress vs. shear strain are plotted for each test. In each plot, the yield stress is determined by selecting the point on the diagram, the tangent of which is having a slope of 5% less than the slope of the elastic portion of the diagram. The geometric locus of these selected points constitutes a single yield stress curve on σ1-σ2 principal plane. The resulting yield stress curve is plotted as an approximate ellipse which resembles Tsai-Hill failure criterion. The results attained in this work, compare well with the results which are readily available in the literature.

  18. Rapid measurement of the yield stress of anaerobically-digested solid waste using slump tests.

    PubMed

    Garcia-Bernet, D; Loisel, D; Guizard, G; Buffière, P; Steyer, J P; Escudié, R

    2011-04-01

    The anaerobic digestion of solid waste is usually performed using dry or semi-dry technology. Incoming waste and fermenting digestate are pasty media and thus, at the industrial scale, their suitability for pumping and mixing is a prerequisite at the industrial scale. However, their rheology has been poorly characterised in the literature because there is no suitable experimental system for analysing heterogeneous media composed of coarse particles. We have developed a practical rheometrical test, a "slump test", for the analysis of actual digested solid waste. It makes it possible to estimate yield stress from the final slump height. From the slump behavior, we conclude that digestates behave as visco-elastic materials. The yield stress of different digested waste was measured between 200 and 800Pa. We show that the media containing smaller particles or with higher moisture content are characterised by smaller yield stresses. This study thus demonstrates the impact of the origin of the digestate on the yield stress. Copyright © 2010 Elsevier Ltd. All rights reserved.

  19. Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting

    PubMed Central

    Mouser, Vivian H. M.; Melchels, Ferry P.W.; Visser, Jetze; Dhert, Wouter J.A.; Gawlitta, Debby; Malda, Jos

    2016-01-01

    Bioprinting of chondrocyte-laden hydrogels facilitates the fabrication of constructs with controlled organization and shape for e.g. articular cartilage implants. Gelatin-methacryloyl (gelMA) supplemented with gellan gum is a promising bio-ink. However, the rheological properties governing the printing process, and the influence of gellan gum on the mechanical properties and chondrogenesis of the blend, are still unknown. Here, we investigated the suitability of gelMA/gellan for cartilage bioprinting. Multiple concentrations, ranging from 3-25% gelMA with 0-1.5% gellan gum, were evaluated for their printability, defined as the ability to form filaments and to incorporate cells at 15-37°C. To support the printability assessment, yield stress and viscosity of the hydrogels were measured. Stiffness of UV-cured constructs, as well as cartilage-like tissue formation by embedded chondrocytes, were determined in vitro. A large range of gelMA/gellan concentrations were printable with inclusion of cells and formed the bioprinting window. Addition of gellan gum improved filament deposition by inducing yielding behavior, increased construct stiffness, and supported chondrogenesis. High gellan gum concentrations, however, did compromise cartilage matrix production and distribution, and even higher concentrations resulted in too high yield stresses to allow cell encapsulation. This study demonstrates the high potential of gelMA/gellan blends for cartilage bioprinting and identifies yield stress as dominant factor for bioprintability. PMID:27431733

  20. Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting.

    PubMed

    Mouser, Vivian H M; Melchels, Ferry P W; Visser, Jetze; Dhert, Wouter J A; Gawlitta, Debby; Malda, Jos

    2016-07-19

    Bioprinting of chondrocyte-laden hydrogels facilitates the fabrication of constructs with controlled organization and shape e.g. for articular cartilage implants. Gelatin-methacryloyl (gelMA) supplemented with gellan gum is a promising bio-ink. However, the rheological properties governing the printing process, and the influence of gellan gum on the mechanical properties and chondrogenesis of the blend, are still unknown. Here, we investigated the suitability of gelMA/gellan for cartilage bioprinting. Multiple concentrations, ranging from 3% to 20% gelMA with 0%-1.5% gellan gum, were evaluated for their printability, defined as the ability to form filaments and to incorporate cells at 15 °C-37 °C. To support the printability assessment, yield stress and viscosity of the hydrogels were measured. Stiffness of UV-cured constructs, as well as cartilage-like tissue formation by embedded chondrocytes, were determined in vitro. A large range of gelMA/gellan concentrations were printable with inclusion of cells and formed the bioprinting window. The addition of gellan gum improved filament deposition by inducing yielding behavior, increased construct stiffness and supported chondrogenesis. High gellan gum concentrations, however, did compromise cartilage matrix production and distribution, and even higher concentrations resulted in too high yield stresses to allow cell encapsulation. This study demonstrates the high potential of gelMA/gellan blends for cartilage bioprinting and identifies yield stress as a dominant factor for bioprintability.

  1. Effect of drought and heat stresses on plant growth and yield: a review

    NASA Astrophysics Data System (ADS)

    Lipiec, J.; Doussan, C.; Nosalewicz, A.; Kondracka, K.

    2013-12-01

    Drought and heat stresses are important threat limitations to plant growth and sustainable agriculture worldwide. Our objective is to provide a review of plant responses and adaptations to drought and elevated temperature including roots, shoots, and final yield and management approaches for alleviating adverse effects of the stresses based mostly on recent literature. The sections of the paper deal with plant responses including root growth, transpiration, photosynthesis, water use efficiency, phenotypic flexibility, accumulation of compounds of low molecular mass (eg proline and gibberellins), and expression of some genes and proteins for increasing the tolerance to the abiotic stresses. Soil and crop management practices to alleviate negative effects of drought and heat stresses are also discussed. Investigations involving determination of plant assimilate partitioning, phenotypic plasticity, and identification of most stress-tolerant plant genotypes are essential for understanding the complexity of the responses and for future plant breeding. The adverse effects of drought and heat stress can be mitigated by soil management practices, crop establishment, and foliar application of growth regulators by maintaining an appropriate level of water in the leaves due to osmotic adjustment and stomatal performance.

  2. Raising yield potential in wheat: increasing photosynthesis capacity and efficiency

    USDA-ARS?s Scientific Manuscript database

    Increasing wheat yields to help to ensure food security is a major challenge. Meeting this challenge requires a quantum improvement in the yield potential of wheat. Past increases in yield potential have largely resulted from improvements in harvest index not through increased biomass. Further large...

  3. Shear induced migration of particles in a yield stress fluid: experiment

    NASA Astrophysics Data System (ADS)

    Hormozi, Sarah; Gholami, Mohammad; Rashedi, Ahmadreza; Lenoir, Nicolas; Ovarlez, Guillaume

    2017-11-01

    We have performed rheometry coupled with X-ray radiography in a narrow gap Couette cell filled with a suspension of spherical particles in a yield stress fluid. In this setup, the shear rate is discontinuous changing from a constant value in the gap to zero in the reservoir located at the top. This shear rate inhomogeneity results in the migration of particles from the gap to the reservoir, so-called Shear Induced Migration (SIM). The rheometry results give us insight into understanding the bulk rheology in the presence of shear rate and solid volume fraction inhomogeneities. In addition to that, our recent X-ray radiography technique (Gholami et al., JOR. 2017) provides detailed information about the evolution of the solid volume fraction in the domain. These measurements allow us to refine the recent continuum model frameworks (Hormozi & Frigaard, JFM 2017) for SIM of particles in a yield stress suspending fluid. We show that complex rheology of the yield stress suspending fluid and formation of the islands of unyielded regions in the reservoir strongly affects the SIM of particles. This feature is absent when we deal with a Newtonian suspending fluid. NSF (Grant No. CBET-1554044- CAREER), ACS PRF (Grant No. 55661-DNI9).

  4. Yield stress and scaling of polyelectrolyte multilayer modified suspensions: effect of polyelectrolyte conformation during multilayer assembly.

    PubMed

    Hess, Andreas; Aksel, Nuri

    2013-09-10

    The yield stress of polyelectrolyte multilayer modified suspensions exhibits a surprising dependence on the polyelectrolyte conformation of multilayer films. The rheological data scale onto a universal master curve for each polyelectrolyte conformation as the particle volume fraction, φ, and the ionic strength of the background fluid, I, are varied. It is shown that rough films with highly coiled, brushy polyelectrolytes significantly enhance the yield stress. Moreover, via the ionic strength I of the background fluid, the dynamic yield stress of brushy polyelectrolyte multilayers can be finely adjusted over 2 decades.

  5. Global crop yield response to extreme heat stress under multiple climate change futures

    NASA Astrophysics Data System (ADS)

    Deryng, D.; Conway, D.; Ramankutty, N.; Price, J.; Warren, R.

    2014-12-01

    Extreme heat stress during the crop reproductive period can be critical for crop productivity. Projected changes in the frequency and severity of extreme climatic events are expected to negatively impact crop yields and global food production. This study applies the global crop model PEGASUS to quantify, for the first time at the global scale, impacts of extreme heat stress on maize, spring wheat and soybean yields resulting from 72 climate change scenarios for the 21st century. Our results project maize to face progressively worse impacts under a range of RCPs but spring wheat and soybean to improve globally through to the 2080s due to CO2 fertilization effects, even though parts of the tropic and sub-tropic regions could face substantial yield declines. We find extreme heat stress at anthesis (HSA) by the 2080s (relative to the 1980s) under RCP 8.5, taking into account CO2 fertilization effects, could double global losses of maize yield (dY = -12.8 ± 6.7% versus -7.0 ± 5.3% without HSA), reduce projected gains in spring wheat yield by half (dY = 34.3 ± 13.5% versus 72.0 ± 10.9% without HSA) and in soybean yield by a quarter (dY = 15.3 ± 26.5% versus 20.4 ± 22.1% without HSA). The range reflects uncertainty due to differences between climate model scenarios; soybean exhibits both positive and negative impacts, maize is generally negative and spring wheat generally positive. Furthermore, when assuming CO2 fertilization effects to be negligible, we observe drastic climate mitigation policy as in RCP 2.6 could avoid more than 80% of the global average yield losses otherwise expected by the 2080s under RCP 8.5. We show large disparities in climate impacts across regions and find extreme heat stress adversely affects major producing regions and lower income countries.

  6. Global crop yield response to extreme heat stress under multiple climate change futures

    NASA Astrophysics Data System (ADS)

    Deryng, Delphine; Conway, Declan; Ramankutty, Navin; Price, Jeff; Warren, Rachel

    2014-03-01

    Extreme heat stress during the crop reproductive period can be critical for crop productivity. Projected changes in the frequency and severity of extreme climatic events are expected to negatively impact crop yields and global food production. This study applies the global crop model PEGASUS to quantify, for the first time at the global scale, impacts of extreme heat stress on maize, spring wheat and soybean yields resulting from 72 climate change scenarios for the 21st century. Our results project maize to face progressively worse impacts under a range of RCPs but spring wheat and soybean to improve globally through to the 2080s due to CO2 fertilization effects, even though parts of the tropic and sub-tropic regions could face substantial yield declines. We find extreme heat stress at anthesis (HSA) by the 2080s (relative to the 1980s) under RCP 8.5, taking into account CO2 fertilization effects, could double global losses of maize yield (ΔY = -12.8 ± 6.7% versus - 7.0 ± 5.3% without HSA), reduce projected gains in spring wheat yield by half (ΔY = 34.3 ± 13.5% versus 72.0 ± 10.9% without HSA) and in soybean yield by a quarter (ΔY = 15.3 ± 26.5% versus 20.4 ± 22.1% without HSA). The range reflects uncertainty due to differences between climate model scenarios; soybean exhibits both positive and negative impacts, maize is generally negative and spring wheat generally positive. Furthermore, when assuming CO2 fertilization effects to be negligible, we observe drastic climate mitigation policy as in RCP 2.6 could avoid more than 80% of the global average yield losses otherwise expected by the 2080s under RCP 8.5. We show large disparities in climate impacts across regions and find extreme heat stress adversely affects major producing regions and lower income countries.

  7. Can stress in farm animals increase food safety risk?

    PubMed

    Rostagno, Marcos H

    2009-09-01

    All farm animals will experience some level of stress during their lives. Stress reduces the fitness of an animal, which can be expressed through failure to achieve production performance standards, or through disease and death. Stress in farm animals can also have detrimental effects on the quality of food products. However, although a common assumption of a potential effect of stress on food safety exists, little is actually known about how this interaction may occur. The aim of this review was to examine the current knowledge of the potential impact of stress in farm animals on food safety risk. Colonization of farm animals by enteric pathogens such as Escherichia coli O157:H7, Salmonella, and Campylobacter, and their subsequent dissemination into the human food chain are a major public health and economic concern for the food industries. This review shows that there is increasing evidence to demonstrate that stress can have a significant deleterious effect on food safety through a variety of potential mechanisms. However, as the impact of stress is difficult to precisely determine, it is imperative that the issue receives more research attention in the interests of optimizing animal welfare and minimizing losses in product yield and quality, as well as to food safety risks to consumers. While there is some evidence linking stress with pathogen carriage and shedding in farm animals, the mechanisms underlying this effect have not been fully elucidated. Understanding when pathogen loads on the farm are the highest or when animals are most susceptible to infection will help identifying times when intervention strategies for pathogen control may be most effective, and consequently, increase the safety of food of animal origin.

  8. Yield Stress Model for Molten Composition B-3

    NASA Astrophysics Data System (ADS)

    Davis, Stephen; Zerkle, David

    2017-06-01

    Composition B-3 (Comp B-3) is a melt-castable explosive composed of 60/40 wt% RDX/TNT (hexahydro-1,3,5-trinitro-1,3,5-triazine/2,4,6-trinitrotoluene). During casting operations thermal conditions are controlled which along with the low melting point of TNT and the insensitivity of the mixture to external stimuli leading to safe use. Outside these standard operating conditions a more rigorous model of Comp B-3 rheological properties is necessary to model thermal transport as Comp B-3 evolves from quiescent solid through vaporization/decomposition upon heating. One particular rheological phenomena of interest is Bingham plasticity, where a material behaves as a quiescent solid unless a sufficient load is applied, resulting in fluid flow. In this study falling ball viscometer data is used to model the change in Bingham plastic yield stresses as a function of RDX particle volume fraction; a function of temperature. Results show the yield stress of Comp B-3 (τy) follows the expression τy = B ϕ -ϕc N , where Φ and Φc are the volume fraction of RDX and a critical volume fraction, respectively and B and N are experimentally evaluated constants.

  9. Metastability at the Yield-Stress Transition in Soft Glasses

    NASA Astrophysics Data System (ADS)

    Lulli, Matteo; Benzi, Roberto; Sbragaglia, Mauro

    2018-04-01

    We study the solid-to-liquid transition in a two-dimensional fully periodic soft-glassy model with an imposed spatially heterogeneous stress. The model we consider consists of droplets of a dispersed phase jammed together in a continuous phase. When the peak value of the stress gets close to the yield stress of the material, we find that the whole system intermittently tunnels to a metastable "fluidized" state, which relaxes back to a metastable "solid" state by means of an elastic-wave dissipation. This macroscopic scenario is studied through the microscopic displacement field of the droplets, whose time statistics displays a remarkable bimodality. Metastability is rooted in the existence, in a given stress range, of two distinct stable rheological branches, as well as long-range correlations (e.g., large dynamic heterogeneity) developed in the system. Finally, we show that a similar behavior holds for a pressure-driven flow, thus suggesting possible experimental tests.

  10. Optimization of MR fluid Yield stress using Taguchi Method and Response Surface Methodology Techniques

    NASA Astrophysics Data System (ADS)

    Mangal, S. K.; Sharma, Vivek

    2018-02-01

    Magneto rheological fluids belong to a class of smart materials whose rheological characteristics such as yield stress, viscosity etc. changes in the presence of applied magnetic field. In this paper, optimization of MR fluid constituents is obtained with on-state yield stress as response parameter. For this, 18 samples of MR fluids are prepared using L-18 Orthogonal Array. These samples are experimentally tested on a developed & fabricated electromagnet setup. It has been found that the yield stress of MR fluid mainly depends on the volume fraction of the iron particles and type of carrier fluid used in it. The optimal combination of the input parameters for the fluid are found to be as Mineral oil with a volume percentage of 67%, iron powder of 300 mesh size with a volume percentage of 32%, oleic acid with a volume percentage of 0.5% and tetra-methyl-ammonium-hydroxide with a volume percentage of 0.7%. This optimal combination of input parameters has given the on-state yield stress as 48.197 kPa numerically. An experimental confirmation test on the optimized MR fluid sample has been then carried out and the response parameter thus obtained has found matching quite well (less than 1% error) with the numerically obtained values.

  11. Ectopic expression of specific GA2 oxidase mutants promotes yield and stress tolerance in rice.

    PubMed

    Lo, Shuen-Fang; Ho, Tuan-Hua David; Liu, Yi-Lun; Jiang, Mirng-Jier; Hsieh, Kun-Ting; Chen, Ku-Ting; Yu, Lin-Chih; Lee, Miin-Huey; Chen, Chi-Yu; Huang, Tzu-Pi; Kojima, Mikiko; Sakakibara, Hitoshi; Chen, Liang-Jwu; Yu, Su-May

    2017-07-01

    A major challenge of modern agricultural biotechnology is the optimization of plant architecture for enhanced productivity, stress tolerance and water use efficiency (WUE). To optimize plant height and tillering that directly link to grain yield in cereals and are known to be tightly regulated by gibberellins (GAs), we attenuated the endogenous levels of GAs in rice via its degradation. GA 2-oxidase (GA2ox) is a key enzyme that inactivates endogenous GAs and their precursors. We identified three conserved domains in a unique class of C 20 GA2ox, GA2ox6, which is known to regulate the architecture and function of rice plants. We mutated nine specific amino acids in these conserved domains and observed a gradient of effects on plant height. Ectopic expression of some of these GA2ox6 mutants moderately lowered GA levels and reprogrammed transcriptional networks, leading to reduced plant height, more productive tillers, expanded root system, higher WUE and photosynthesis rate, and elevated abiotic and biotic stress tolerance in transgenic rice. Combinations of these beneficial traits conferred not only drought and disease tolerance but also increased grain yield by 10-30% in field trials. Our studies hold the promise of manipulating GA levels to substantially improve plant architecture, stress tolerance and grain yield in rice and possibly in other major crops. © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  12. Detrimental effect of selection for milk yield on genetic tolerance to heat stress in purebred Zebu cattle: Genetic parameters and trends.

    PubMed

    Santana, M L; Pereira, R J; Bignardi, A B; Filho, A E Vercesi; Menéndez-Buxadera, A; El Faro, L

    2015-12-01

    In an attempt to determine the possible detrimental effects of continuous selection for milk yield on the genetic tolerance of Zebu cattle to heat stress, genetic parameters and trends of the response to heat stress for 86,950 test-day (TD) milk yield records from 14,670 first lactations of purebred dairy Gir cows were estimated. A random regression model with regression on days in milk (DIM) and temperature-humidity index (THI) values was applied to the data. The most detrimental effect of THI on milk yield was observed in the stage of lactation with higher milk production, DIM 61 to 120 (-0.099kg/d per THI). Although modest variations were observed for the THI scale, a reduction in additive genetic variance as well as in permanent environmental and residual variance was observed with increasing THI values. The heritability estimates showed a slight increase with increasing THI values for any DIM. The correlations between additive genetic effects across the THI scale showed that, for most of the THI values, genotype by environment interactions due to heat stress were less important for the ranking of bulls. However, for extreme THI values, this type of genotype by environment interaction may lead to an important error in selection. As a result of the selection for milk yield practiced in the dairy Gir population for 3 decades, the genetic trend of cumulative milk yield was significantly positive for production in both high (51.81kg/yr) and low THI values (78.48kg/yr). However, the difference between the breeding values of animals at high and low THI may be considered alarming (355kg in 2011). The genetic trends observed for the regression coefficients related to general production level (intercept of the reaction norm) and specific ability to respond to heat stress (slope of the reaction norm) indicate that the dairy Gir population is heading toward a higher production level at the expense of lower tolerance to heat stress. These trends reflect the genetic

  13. Heat waves imposed during early pod development in soybean (Glycine max) cause significant yield loss despite a rapid recovery from oxidative stress.

    PubMed

    Siebers, Matthew H; Yendrek, Craig R; Drag, David; Locke, Anna M; Rios Acosta, Lorena; Leakey, Andrew D B; Ainsworth, Elizabeth A; Bernacchi, Carl J; Ort, Donald R

    2015-08-01

    Heat waves already have a large impact on crops and are predicted to become more intense and more frequent in the future. In this study, heat waves were imposed on soybean using infrared heating technology in a fully open-air field experiment. Five separate heat waves were applied to field-grown soybean (Glycine max) in central Illinois, three in 2010 and two in 2011. Thirty years of historical weather data from Illinois were analyzed to determine the length and intensity of a regionally realistic heat wave resulting in experimental heat wave treatments during which day and night canopy temperatures were elevated 6 °C above ambient for 3 days. Heat waves were applied during early or late reproductive stages to determine whether and when heat waves had an impact on carbon metabolism and seed yield. By the third day of each heat wave, net photosynthesis (A), specific leaf weight (SLW), and leaf total nonstructural carbohydrate concentration (TNC) were decreased, while leaf oxidative stress was increased. However, A, SLW, TNC, and measures of oxidative stress were no different than the control ca. 12 h after the heat waves ended, indicating rapid physiological recovery from the high-temperature stress. That end of season seed yield was reduced (~10%) only when heat waves were applied during early pod developmental stages indicates the yield loss had more to do with direct impacts of the heat waves on reproductive process than on photosynthesis. Soybean was unable to mitigate yield loss after heat waves given during late reproductive stages. This study shows that short high-temperature stress events that reduce photosynthesis and increase oxidative stress resulted in significant losses to soybean production in the Midwest, U.S. The study also suggests that to mitigate heat wave-induced yield loss, soybean needs improved reproductive and photosynthetic tolerance to high but increasingly common temperatures. Published 2015. This article is a U.S. Government work and is

  14. Grain-size-yield stress relationship: Analysis and computation

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Meyers, M.A.; Benson, D.J.; Fu, H.H.

    1999-07-01

    The seminal contributions of Julia Weertman to the understanding of the mechanical properties of nanocrystalline materials will be briefly outlined. A constitutive equation predicting the effect of grain size on the yield stress of metals, based on the model proposed by M.A. Meyers and E. Ashworth, is discussed and extended to the nanocrystalline regime. At large grain sizes, it has the Hall-Petch form, and in the nanocrystalline domain the slope gradually decreases until it asymptotically approaches the flow stress of the grain boundaries. The material is envisaged as a composite, comprised of the grain interior, with flow stress {sigma}{sub fB},more » and grain boundary work-hardened layer, with flow stress {sigma}{sub fGB}. Three principal factors contribute to the grain-boundary hardening: (1) the grain boundaries act as barriers to plastic flow; (2) the grain boundaries act as dislocation sources; and (3) elastic anisotropy causes additional stresses in grain-boundary surroundings. The predictions of this model are compared with experimental measurements over the mono, micro, and nanocrystalline domains. Computational predictions are made of plastic flow as a function of grain size incorporating elastic and plastic anisotropy as well as differences of dislocation accumulation rate in grain boundary regions and grain interiors. This is the first plasticity calculation that accounts for grain size effects in a physically-based manner. 58 refs., 7 figs., 1 tab.« less

  15. Boron application improves yield of rice cultivars under high temperature stress during vegetative and reproductive stages.

    PubMed

    Shahid, Mohammad; Nayak, Amaresh Kumar; Tripathi, Rahul; Katara, Jawahar Lal; Bihari, Priyanka; Lal, Banwari; Gautam, Priyanka

    2018-04-12

    It is reported that high temperatures (HT) would cause a marked decrease in world rice production. In tropical regions, high temperatures are a constraint to rice production and the most damaging effect is on spikelet sterility. Boron (B) plays a very important role in the cell wall formation, sugar translocation, and reproduction of the rice crop and could play an important role in alleviating high temperature stress. A pot culture experiment was conducted to study the effect of B application on high temperature tolerance of rice cultivars in B-deficient soil. The treatments comprised of four boron application treatments viz. control (B0), soil application of 1 kg B ha -1 (B1), soil application of 2 kg B ha -1 (B2), and foliar spray of 0.2% B (Bfs); three rice cultivars viz. Annapurna (HT stress tolerant), Naveen, and Shatabdi (both HT stress susceptible); and three temperature regimes viz. ambient (AT), HT at vegetative stage (HTV), and HT at reproductive stage (HTR). The results revealed that high temperature stress during vegetative or flowering stage reduced grain yield of rice cultivars mainly because of low pollen viability and spikelet fertility. The effects of high temperature on the spikelet fertility and grain filling varied among cultivars and the growth stages of plant when exposed to the high temperature stress. Under high temperature stress, the tolerant cultivar displays higher cell membrane stability, less accumulation of osmolytes, more antioxidant enzyme activities, and higher pollen viability and spikelet fertility than the susceptible cultivars. In the present work, soil application of boron was effective in reducing the negative effects of high temperature both at vegetative and reproductive stages. Application of B results into higher grain yield under both ambient and high temperature condition over control for all the three cultivars; however, more increase was observed for the susceptible cultivar over the tolerant one. The results

  16. Boron application improves yield of rice cultivars under high temperature stress during vegetative and reproductive stages

    NASA Astrophysics Data System (ADS)

    Shahid, Mohammad; Nayak, Amaresh Kumar; Tripathi, Rahul; Katara, Jawahar Lal; Bihari, Priyanka; Lal, Banwari; Gautam, Priyanka

    2018-04-01

    It is reported that high temperatures (HT) would cause a marked decrease in world rice production. In tropical regions, high temperatures are a constraint to rice production and the most damaging effect is on spikelet sterility. Boron (B) plays a very important role in the cell wall formation, sugar translocation, and reproduction of the rice crop and could play an important role in alleviating high temperature stress. A pot culture experiment was conducted to study the effect of B application on high temperature tolerance of rice cultivars in B-deficient soil. The treatments comprised of four boron application treatments viz. control (B0), soil application of 1 kg B ha-1 (B1), soil application of 2 kg B ha-1 (B2), and foliar spray of 0.2% B (Bfs); three rice cultivars viz. Annapurna (HT stress tolerant), Naveen, and Shatabdi (both HT stress susceptible); and three temperature regimes viz. ambient (AT), HT at vegetative stage (HTV), and HT at reproductive stage (HTR). The results revealed that high temperature stress during vegetative or flowering stage reduced grain yield of rice cultivars mainly because of low pollen viability and spikelet fertility. The effects of high temperature on the spikelet fertility and grain filling varied among cultivars and the growth stages of plant when exposed to the high temperature stress. Under high temperature stress, the tolerant cultivar displays higher cell membrane stability, less accumulation of osmolytes, more antioxidant enzyme activities, and higher pollen viability and spikelet fertility than the susceptible cultivars. In the present work, soil application of boron was effective in reducing the negative effects of high temperature both at vegetative and reproductive stages. Application of B results into higher grain yield under both ambient and high temperature condition over control for all the three cultivars; however, more increase was observed for the susceptible cultivar over the tolerant one. The results suggest

  17. On the residual yield stress of shocked metals

    NASA Astrophysics Data System (ADS)

    Chapman, David; Eakins, Daniel; Savinykh, Andrey; Garkushin, Gennady; Kanel, Gennady; Razorenov, Sergey

    2013-06-01

    The measurement of the free-surface velocity is commonly employed in planar shock-compression experiments. It is known that the peak free-surface velocity of a shocked elastic-plastic material should be slightly less than twice the particle velocity behind shock front; this difference being proportional to the yield stress. Precise measurement of the free-surface velocity can be a rich source of information on the effects of time and strain on material hardening or softening. With this objective, we performed comparative measurements of the free-surface velocity of shock loaded aluminium AD1 and magnesium alloy Ma2 samples of various thicknesses in the range 0.2 mm to 5 mm. We observed the expected hysteresis in the elastic-plastic compression-unloading cycle for both AD1 and Ma2; where qualitatively the peak free-surface velocity increased with increasing specimen thickness. However, the relative change in magnitude of hysteresis as function of specimen thickness observed for the Ma2 alloy was smaller than expected given the large observed change in precursor magnitude. We propose that softening due to multiplication of dislocations is relatively large in Ma2 and results in a smaller hysteresis in the elastic-plastic cycle.

  18. Root carboxylate exudation capacity under phosphorus stress does not improve grain yield in green gram.

    PubMed

    Pandey, Renu; Meena, Surendra Kumar; Krishnapriya, Vengavasi; Ahmad, Altaf; Kishora, Naval

    2014-06-01

    Genetic variability in carboxylate exudation capacity along with improved root traits was a key mechanism for P-efficient green gram genotype to cope with P-stress but it did not increase grain yield. This study evaluates genotypic variability in green gram for total root carbon exudation under low phosphorus (P) using (14)C and its relationship with root exuded carboxylates, growth and yield potential in contrasting genotypes. Forty-four genotypes grown hydroponically with low (2 μM) and sufficient (100 μM) P concentrations were exposed to (14)CO2 to screen for total root carbon exudation. Contrasting genotypes were employed to study carboxylate exudation and their performance in soil at two P levels. Based on relative (14)C exudation and biomass, genotypes were categorized. Carboxylic acids were measured in exudates and root apices of contrasting genotypes belonging to efficient and inefficient categories. Oxalic and citric acids were released into the medium under low-P. PDM-139 (efficient) was highly efficient in carboxylate exudation as compared to ML-818 (inefficient). In low soil P, the reduction in biomass was higher in ML-818 as compared to PDM-139. Total leaf area and photosynthetic rate averaged for genotypes increased by 71 and 41 %, respectively, with P fertilization. Significantly, higher root surface area and volume were observed in PDM-139 under low soil P. Though the grain yield was higher in ML-818, the total plant biomass was significantly higher in PDM-139 indicating improved P uptake and its efficient translation into biomass. The higher carboxylate exudation capacity and improved root traits in the later genotype might be the possible adaptive mechanisms to cope with P-stress. However, it is not necessary that higher root exudation would result in higher grain yield.

  19. Interactive effects of pests increase seed yield.

    PubMed

    Gagic, Vesna; Riggi, Laura Ga; Ekbom, Barbara; Malsher, Gerard; Rusch, Adrien; Bommarco, Riccardo

    2016-04-01

    Loss in seed yield and therefore decrease in plant fitness due to simultaneous attacks by multiple herbivores is not necessarily additive, as demonstrated in evolutionary studies on wild plants. However, it is not clear how this transfers to crop plants that grow in very different conditions compared to wild plants. Nevertheless, loss in crop seed yield caused by any single pest is most often studied in isolation although crop plants are attacked by many pests that can cause substantial yield losses. This is especially important for crops able to compensate and even overcompensate for the damage. We investigated the interactive impacts on crop yield of four insect pests attacking different plant parts at different times during the cropping season. In 15 oilseed rape fields in Sweden, we estimated the damage caused by seed and stem weevils, pollen beetles, and pod midges. Pest pressure varied drastically among fields with very low correlation among pests, allowing us to explore interactive impacts on yield from attacks by multiple species. The plant damage caused by each pest species individually had, as expected, either no, or a negative impact on seed yield and the strongest negative effect was caused by pollen beetles. However, seed yield increased when plant damage caused by both seed and stem weevils was high, presumably due to the joint plant compensatory reaction to insect attack leading to overcompensation. Hence, attacks by several pests can change the impact on yield of individual pest species. Economic thresholds based on single species, on which pest management decisions currently rely, may therefore result in economically suboptimal choices being made and unnecessary excessive use of insecticides.

  20. Expression of Arabidopsis glycine-rich RNA-binding protein AtGRP2 or AtGRP7 improves grain yield of rice (Oryza sativa) under drought stress conditions.

    PubMed

    Yang, Deok Hee; Kwak, Kyung Jin; Kim, Min Kyung; Park, Su Jung; Yang, Kwang-Yeol; Kang, Hunseung

    2014-01-01

    Although posttranscriptional regulation of RNA metabolism is increasingly recognized as a key regulatory process in plant response to environmental stresses, reports demonstrating the importance of RNA metabolism control in crop improvement under adverse environmental stresses are severely limited. To investigate the potential use of RNA-binding proteins (RBPs) in developing stress-tolerant transgenic crops, we generated transgenic rice plants (Oryza sativa) that express Arabidopsis thaliana glycine-rich RBP (AtGRP) 2 or 7, which have been determined to harbor RNA chaperone activity and confer stress tolerance in Arabidopsis, and analyzed the response of the transgenic rice plants to abiotic stresses. AtGRP2- or AtGRP7-expressing transgenic rice plants displayed similar phenotypes comparable with the wild-type plants under high salt or cold stress conditions. By contrast, AtGRP2- or AtGRP7-expressing transgenic rice plants showed much higher recovery rates and grain yields compared with the wild-type plants under drought stress conditions. The higher grain yield of the transgenic rice plants was due to the increases in filled grain numbers per panicle. Collectively, the present results show the importance of posttranscriptional regulation of RNA metabolism in plant response to environmental stress and suggest that GRPs can be utilized to improve the yield potential of crops under stress conditions. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  1. Wildlife-friendly farming increases crop yield: evidence for ecological intensification.

    PubMed

    Pywell, Richard F; Heard, Matthew S; Woodcock, Ben A; Hinsley, Shelley; Ridding, Lucy; Nowakowski, Marek; Bullock, James M

    2015-10-07

    Ecological intensification has been promoted as a means to achieve environmentally sustainable increases in crop yields by enhancing ecosystem functions that regulate and support production. There is, however, little direct evidence of yield benefits from ecological intensification on commercial farms growing globally important foodstuffs (grains, oilseeds and pulses). We replicated two treatments removing 3 or 8% of land at the field edge from production to create wildlife habitat in 50-60 ha patches over a 900 ha commercial arable farm in central England, and compared these to a business as usual control (no land removed). In the control fields, crop yields were reduced by as much as 38% at the field edge. Habitat creation in these lower yielding areas led to increased yield in the cropped areas of the fields, and this positive effect became more pronounced over 6 years. As a consequence, yields at the field scale were maintained--and, indeed, enhanced for some crops--despite the loss of cropland for habitat creation. These results suggested that over a 5-year crop rotation, there would be no adverse impact on overall yield in terms of monetary value or nutritional energy. This study provides a clear demonstration that wildlife-friendly management which supports ecosystem services is compatible with, and can even increase, crop yields. © 2015 The Authors.

  2. Wildlife-friendly farming increases crop yield: evidence for ecological intensification

    PubMed Central

    Pywell, Richard F.; Heard, Matthew S.; Woodcock, Ben A.; Hinsley, Shelley; Ridding, Lucy; Nowakowski, Marek; Bullock, James M.

    2015-01-01

    Ecological intensification has been promoted as a means to achieve environmentally sustainable increases in crop yields by enhancing ecosystem functions that regulate and support production. There is, however, little direct evidence of yield benefits from ecological intensification on commercial farms growing globally important foodstuffs (grains, oilseeds and pulses). We replicated two treatments removing 3 or 8% of land at the field edge from production to create wildlife habitat in 50–60 ha patches over a 900 ha commercial arable farm in central England, and compared these to a business as usual control (no land removed). In the control fields, crop yields were reduced by as much as 38% at the field edge. Habitat creation in these lower yielding areas led to increased yield in the cropped areas of the fields, and this positive effect became more pronounced over 6 years. As a consequence, yields at the field scale were maintained—and, indeed, enhanced for some crops—despite the loss of cropland for habitat creation. These results suggested that over a 5-year crop rotation, there would be no adverse impact on overall yield in terms of monetary value or nutritional energy. This study provides a clear demonstration that wildlife-friendly management which supports ecosystem services is compatible with, and can even increase, crop yields. PMID:26423846

  3. Stress regularity in quasi-static perfect plasticity with a pressure dependent yield criterion

    NASA Astrophysics Data System (ADS)

    Babadjian, Jean-François; Mora, Maria Giovanna

    2018-04-01

    This work is devoted to establishing a regularity result for the stress tensor in quasi-static planar isotropic linearly elastic - perfectly plastic materials obeying a Drucker-Prager or Mohr-Coulomb yield criterion. Under suitable assumptions on the data, it is proved that the stress tensor has a spatial gradient that is locally squared integrable. As a corollary, the usual measure theoretical flow rule is expressed in a strong form using the quasi-continuous representative of the stress.

  4. Food security: increasing yield and improving resource use efficiency.

    PubMed

    Parry, Martin A J; Hawkesford, Malcolm J

    2010-11-01

    Food production and security will be a major issue for supplying an increasing world population. The problem will almost certainly be exacerbated by climate change. There is a projected need to double food production by 2050. In recent times, the trend has been for incremental modest yield increases for most crops. There is an urgent need to develop integrated and sustainable approaches that will significantly increase both production per unit land area and the resource use efficiency of crops. This review considers some key processes involved in plant growth and development with some examples of ways in which molecular technology, plant breeding and genetics may increase the yield and resource use efficiency of wheat. The successful application of biotechnology to breeding is essential to provide the major increases in production required. However, each crop and each specific agricultural situation presents specific requirements and targets for optimisation. Some increases in production will come about as new varieties are developed which are able to produce satisfactory crops on marginal land presently not considered appropriate for arable crops. Other new varieties will be developed to increase both yield and resource use efficiency on the best land.

  5. The Effect of a Yield Stress on the Drainage of the Thin Film Between Two Colliding Newtonian Drops

    NASA Astrophysics Data System (ADS)

    Goel, Sachin; Ramachandran, Arun

    2016-11-01

    Coalescence of drops immersed in fluids possessing a yield stress has been of interest to many industries such as the oil extraction, cosmetics and food industries. Unfortunately, a theoretical understanding of the drainage of the thin film of Bingham fluid (a model yield stress fluid) that develops between two drops undergoing a collision is still lacking, with the exception of two prior studies that make ad-hoc assumptions about the film shape. In this work, we examine this problem via a combination of scaling analysis and numerical simulations based on the lubrication analysis. There are four key features of the film drainage process of Bingham fluids. First, the introduction of a yield stress in the suspending fluid retards the drainage process relative to Newtonian fluid of the same viscosity. Second, the drainage time shows a minimum with respect to the capillary number. Third, the effect of yield stress on the drainage process becomes more pronounced at higher capillary numbers and lower Hamaker constant. Lastly, below a critical height, drainage can be arrested completely due to the yield stress. This critical height scales as τ02R3 τ02R3 γ2 γ2 , where τ0 is the yield stress, R is the drop radius and γ is the interfacial tension, and is, surprisingly, independent of the force colliding the drops. This and other distinguishing characteristics of the drainage process will be elucidated in the presentation.

  6. Impact of mild heat stress on dry matter intake, milk yield and milk composition in mid-lactation Holstein dairy cows in a temperate climate.

    PubMed

    Gorniak, Tobias; Meyer, Ulrich; Südekum, Karl-Heinz; Dänicke, Sven

    2014-01-01

    The aim of the present study was to evaluate the impact of summer temperatures in a temperate climate on mid-lactation Holstein dairy cows. Therefore, a data set was examined comprising five trials with dairy cows conducted at the experimental station of the Friedrich-Loeffler-Institute in Braunschweig, Germany. The temperature-humidity index (THI) was calculated using temperature and humidity data from the barns recorded between January 2010 and July 2012. By using a generalised additive mixed model, the impact of increasing THI on dry matter intake, milk yield and milk composition was evaluated. Dry matter intake and milk yield decreased when THI rose above 60, whilst water intake increased in a linear manner beyond THI 30. Furthermore, milk protein and milk fat content decreased continuously with increasing THI. The present results revealed that heat stress exists in Lower Saxony, Germany. However, further research is necessary to describe the mode of action of heat stress. Especially, mild heat stress has to be investigated in more detail and appropriate heat stress thresholds for temperate climates have to be developed.

  7. Coating flow of an anti-HIV microbicide gel: boundary dilution and yield stress

    NASA Astrophysics Data System (ADS)

    Szeri, Andrew J.; Tasoglu, Savas; Park, Su Chan; Katz, David F.

    2010-11-01

    A recent study has confirmed, for the first time, that a vaginal gel formulation of the antiretroviral drug Tenofovir, when topically applied, significantly inhibits sexual HIV transmission to women [1]. However, the gel for this drug, and anti-HIV microbicide gels in general, have not been designed using an understanding of how gel spreading govern successful drug delivery. Elastohydrodynamic lubrication theory can be applied to model spreading of microbicide gels [2]. Here, we extend our initial analysis: we incorporate a yield stress, and we model the effects of gel dilution due to contact with vaginal fluid produced at the gel-tissue interface. Our model developed in [2] is supplemented with a convective-diffusive transport equation to characterize dilution, and solved using a multi-step scheme in a moving domain. The association between local dilution of gel and rheological properties is obtained experimentally. To model the common yield stress property of gels, we proceed by scaling analysis first. This establishes the conditions for validity of lubrication theory of a shear thinning yield stress fluid. This involves further development of the model in [2], incorporating a biviscosity model.[4pt] [1] Karim, et al., Science, 2010.[0pt] [2] Szeri, et al., Phy. of Fluids, 2008.

  8. Increased Biomass, Seed Yield and Stress Tolerance Is Conferred in Arabidopsis by a Novel Enzyme from the Resurrection Grass Sporobolus stapfianus That Glycosylates the Strigolactone Analogue GR24

    PubMed Central

    Islam, Sharmin; Griffiths, Cara A.; Blomstedt, Cecilia K.; Le, Tuan-Ngoc; Gaff, Donald F.; Hamill, John D.; Neale, Alan D.

    2013-01-01

    Isolation of gene transcripts from desiccated leaf tissues of the resurrection grass, Sporobolus stapfianus, resulted in the identification of a gene, SDG8i, encoding a Group 1 glycosyltransferase (UGT). Here, we examine the effects of introducing this gene, under control of the CaMV35S promoter, into the model plant Arabidopsis thaliana. Results show that Arabidopsis plants constitutively over-expressing SDG8i exhibit enhanced growth, reduced senescence, cold tolerance and a substantial improvement in protoplasmic drought tolerance. We hypothesise that expression of SDG8i in Arabidopsis negatively affects the bioactivity of metabolite/s that mediate/s environmentally-induced repression of cell division and expansion, both during normal development and in response to stress. The phenotype of transgenic plants over-expressing SDG8i suggests modulation in activities of both growth- and stress-related hormones. Plants overexpressing the UGT show evidence of elevated auxin levels, with the enzyme acting downstream of ABA to reduce drought-induced senescence. Analysis of the in vitro activity of the UGT recombinant protein product demonstrates that SDG8i can glycosylate the synthetic strigolactone analogue GR24, evoking a link with strigolactone-related processes in vivo. The large improvements observed in survival of transgenic Arabidopsis plants under cold-, salt- and drought-stress, as well as the substantial increases in growth rate and seed yield under non-stress conditions, indicates that overexpression of SDG8i in crop plants may provide a novel means of increasing plant productivity. PMID:24224034

  9. Expression of an Arabidopsis molybdenum cofactor sulphurase gene in soybean enhances drought tolerance and increases yield under field conditions.

    PubMed

    Li, Yajun; Zhang, Jiachang; Zhang, Juan; Hao, Ling; Hua, Jinping; Duan, Liusheng; Zhang, Mingcai; Li, Zhaohu

    2013-08-01

    LOS5/ABA3 gene encoding molybdenum cofactor sulphurase is involved in aldehyde oxidase (AO) activity in Arabidopsis, which indirectly regulates ABA biosynthesis and increased stress tolerance. Here, we used a constitutive super promoter to drive LOS5/ABA3 overexpression in soybean (Glycine max L.) to enhance drought tolerance in growth chamber and field conditions. Expression of LOS5/ABA3 was up-regulated by drought stress, which led to increasing AO activity and then a notable increase in ABA accumulation. Transgenic soybean under drought stress had reduced water loss by decreased stomatal aperture size and transpiration rate, which alleviated leaf wilting and maintained higher relative water content. Exposed to drought stress, transgenic soybean exhibited reduced cell membrane damage by reducing electrolyte leakage and production of malondialdehyde and promoting proline accumulation and antioxidant enzyme activities. Also, overexpression of LOS5/ABA3 enhanced expression of stress-up-regulated genes. Furthermore, the seed yield of transgenic plants is at least 21% higher than that of wide-type plants under drought stress conditions in the field. These data suggest that overexpression of LOS5/ABA3 could improve drought tolerance in transgenic soybean via enhanced ABA accumulation, which could activate expression of stress-up-regulated genes and cause a series of physiological and biochemical resistant responses. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  10. Drought Tolerance during Reproductive Development is Important for Increasing wheat yield Potential under Climate change in Europe.

    PubMed

    Senapati, Nimai; Stratonovitch, Pierre; Paul, Matthew J; Semenov, Mikhail A

    2018-06-12

    Drought stress during reproductive development could drastically reduce grain number and wheat yield, but quantitative evaluation of such effect is unknown under climate change. The objectives of this study were to a) evaluate potential yield benefits of drought tolerance during reproductive development for wheat ideotypes under climate change in Europe, and b) identify potential cultivar parameters for improvement. We used the Sirius wheat model to optimise drought tolerant (DT) and drought sensitive (DS) wheat ideotypes under future 2050 climate scenario at 13 contrasting sites, representing major wheat growing regions in Europe. Averaged over the sites, DT ideotypes achieved 13.4% greater yield compared to DS, with the double yield stability for DT. However, the performances of the ideotypes were site dependent. Mean yield of DT was 28-37% greater compared to DS in southern Europe. In contrast, no yield difference (≤ 1%) between ideotypes was found in north-western Europe. An intermediate yield benefit of 10-23% was found due to drought tolerance in central and eastern Europe. We conclude that tolerance to drought stress during reproductive development is important for high yield potentials and greater yield stability of wheat under climate change in Europe.

  11. Universal rescaling of flow curves for yield-stress fluids close to jamming

    NASA Astrophysics Data System (ADS)

    Dinkgreve, M.; Paredes, J.; Michels, M. A. J.; Bonn, D.

    2015-07-01

    The experimental flow curves of four different yield-stress fluids with different interparticle interactions are studied near the jamming concentration. By appropriate scaling with the distance to jamming all rheology data can be collapsed onto master curves below and above jamming that meet in the shear-thinning regime and satisfy the Herschel-Bulkley and Cross equations, respectively. In spite of differing interactions in the different systems, master curves characterized by universal scaling exponents are found for the four systems. A two-state microscopic theory of heterogeneous dynamics is presented to rationalize the observed transition from Herschel-Bulkley to Cross behavior and to connect the rheological exponents to microscopic exponents for the divergence of the length and time scales of the heterogeneous dynamics. The experimental data and the microscopic theory are compared with much of the available literature data for yield-stress systems.

  12. Is Yield Increase Sufficient to Achieve Food Security in China?

    PubMed Central

    Wei, Xing; Zhang, Zhao; Shi, Peijun; Wang, Pin; Chen, Yi; Song, Xiao; Tao, Fulu

    2015-01-01

    Increasing demand for food, driven by unprecedented population growth and increasing consumption, will keep challenging food security in China. Although cereal yields have substantially improved during the last three decades, whether it will keep thriving to meet the increasing demand is not known yet. Thus, an integrated analysis on the trends of crop yield and cultivated area is essential to better understand current state of food security in China, especially on county scale. So far, yield stagnation has extensively dominated the main cereal-growing areas across China. Rice yield is facing the most severe stagnation that 53.9% counties tracked in the study have stagnated significantly, followed by maize (42.4%) and wheat (41.9%). As another important element for production sustainability, but often neglected is the planted area patterns. It has been further demonstrated that the loss in productive arable land for rice and wheat have dramatically increased the pressure on achieving food security. Not only a great deal of the planted areas have stagnated since 1980, but also collapsed. 48.4% and 54.4% of rice- and wheat-growing counties have lost their cropland areas to varying degrees. Besides, 27.6% and 35.8% of them have retrograded below the level of the 1980s. The combined influence (both loss in yield and area) has determined the crop sustainable production in China to be pessimistic for rice and wheat, and consequently no surprise to find that more than half of counties rank a lower level of production sustainability. Therefore, given the potential yield increase in wheat and maize, as well as substantial area loss of rice and wheat, the possible targeted adaptation measures for both yield and cropping area is required at county scale. Moreover, policies on food trade, alongside advocation of low calorie diets, reducing food loss and waste can help to enhance food security. PMID:25680193

  13. Perturbation of the yield-stress rheology of polymer thin films by nonlinear shear ultrasound.

    PubMed

    Léopoldès, J; Conrad, G; Jia, X

    2015-01-01

    We investigate the nonlinear response of macromolecular thin films subjected to high-amplitude ultrasonic shear oscillation using a sphere-plane contact geometry. At a film thickness comparable to the radius of gyration, we observe the rheological properties intermediate between bulk and boundary nonlinear regimes. As the driving amplitude is increased, these films progressively exhibit oscillatory linear, microslip, and full slip regimes, which can be explained by the modified Coulomb friction law. At highest oscillation amplitudes, the interfacial adhesive failure takes place, being accompanied by a dewettinglike pattern. Moreover, the steady state sliding is investigated in thicker films with imposed shear stresses beyond the yield point. We find that applying high-amplitude shear ultrasound affects not only the yielding threshold but also the sliding velocity at a given shear load. A possible mechanism for the latter effect is discussed.

  14. Material and methods to increase plant growth and yield

    DOEpatents

    Kirst, Matias

    2015-09-15

    The present invention relates to materials and methods for modulating growth rates, yield, and/or resistance to drought conditions in plants. In one embodiment, a method of the invention comprises increasing expression of an hc1 gene (or a homolog thereof that provides for substantially the same activity), or increasing expression or activity of the protein encoded by an hc1 gene thereof, in a plant, wherein expression of the hc1 gene or expression or activity of the protein encoded by an hc1 gene results in increased growth rate, yield, and/or drought resistance in the plant.

  15. Materials and methods to increase plant growth and yield

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kirst, Matias

    The present invention relates to materials and methods for modulating growth rates, yield, and/or resistance to drought conditions in plants. In one embodiment, a method of the invention comprises increasing expression of an hc1 gene (or a homolog thereof that provides for substantially the same activity), or increasing expression or activity of the protein encoded by an hc1 gene thereof, in a plant, wherein expression of the hc1 gene or expression or activity of the protein encoded by an hc1 gene results in increased growth rate, yield, and/or drought resistance in the plant.

  16. Assessing and Exploiting Functional Diversity in Germplasm Pools to Enhance Abiotic Stress Adaptation and Yield in Cereals and Food Legumes

    PubMed Central

    Dwivedi, Sangam L.; Scheben, Armin; Edwards, David; Spillane, Charles; Ortiz, Rodomiro

    2017-01-01

    There is a need to accelerate crop improvement by introducing alleles conferring host plant resistance, abiotic stress adaptation, and high yield potential. Elite cultivars, landraces and wild relatives harbor useful genetic variation that needs to be more easily utilized in plant breeding. We review genome-wide approaches for assessing and identifying alleles associated with desirable agronomic traits in diverse germplasm pools of cereals and legumes. Major quantitative trait loci and single nucleotide polymorphisms (SNPs) associated with desirable agronomic traits have been deployed to enhance crop productivity and resilience. These include alleles associated with variation conferring enhanced photoperiod and flowering traits. Genetic variants in the florigen pathway can provide both environmental flexibility and improved yields. SNPs associated with length of growing season and tolerance to abiotic stresses (precipitation, high temperature) are valuable resources for accelerating breeding for drought-prone environments. Both genomic selection and genome editing can also harness allelic diversity and increase productivity by improving multiple traits, including phenology, plant architecture, yield potential and adaptation to abiotic stresses. Discovering rare alleles and useful haplotypes also provides opportunities to enhance abiotic stress adaptation, while epigenetic variation has potential to enhance abiotic stress adaptation and productivity in crops. By reviewing current knowledge on specific traits and their genetic basis, we highlight recent developments in the understanding of crop functional diversity and identify potential candidate genes for future use. The storage and integration of genetic, genomic and phenotypic information will play an important role in ensuring broad and rapid application of novel genetic discoveries by the plant breeding community. Exploiting alleles for yield-related traits would allow improvement of selection efficiency and

  17. Assessing and Exploiting Functional Diversity in Germplasm Pools to Enhance Abiotic Stress Adaptation and Yield in Cereals and Food Legumes.

    PubMed

    Dwivedi, Sangam L; Scheben, Armin; Edwards, David; Spillane, Charles; Ortiz, Rodomiro

    2017-01-01

    There is a need to accelerate crop improvement by introducing alleles conferring host plant resistance, abiotic stress adaptation, and high yield potential. Elite cultivars, landraces and wild relatives harbor useful genetic variation that needs to be more easily utilized in plant breeding. We review genome-wide approaches for assessing and identifying alleles associated with desirable agronomic traits in diverse germplasm pools of cereals and legumes. Major quantitative trait loci and single nucleotide polymorphisms (SNPs) associated with desirable agronomic traits have been deployed to enhance crop productivity and resilience. These include alleles associated with variation conferring enhanced photoperiod and flowering traits. Genetic variants in the florigen pathway can provide both environmental flexibility and improved yields. SNPs associated with length of growing season and tolerance to abiotic stresses (precipitation, high temperature) are valuable resources for accelerating breeding for drought-prone environments. Both genomic selection and genome editing can also harness allelic diversity and increase productivity by improving multiple traits, including phenology, plant architecture, yield potential and adaptation to abiotic stresses. Discovering rare alleles and useful haplotypes also provides opportunities to enhance abiotic stress adaptation, while epigenetic variation has potential to enhance abiotic stress adaptation and productivity in crops. By reviewing current knowledge on specific traits and their genetic basis, we highlight recent developments in the understanding of crop functional diversity and identify potential candidate genes for future use. The storage and integration of genetic, genomic and phenotypic information will play an important role in ensuring broad and rapid application of novel genetic discoveries by the plant breeding community. Exploiting alleles for yield-related traits would allow improvement of selection efficiency and

  18. Photosynthetic Diffusional Constraints Affect Yield in Drought Stressed Rice Cultivars during Flowering

    PubMed Central

    Lauteri, Marco; Haworth, Matthew; Serraj, Rachid; Monteverdi, Maria Cristina; Centritto, Mauro

    2014-01-01

    Global production of rice (Oryza sativa) grain is limited by water availability and the low ‘leaf-level’ photosynthetic capacity of many cultivars. Oryza sativa is extremely susceptible to water-deficits; therefore, predicted increases in the frequency and duration of drought events, combined with future rises in global temperatures and food demand, necessitate the development of more productive and drought tolerant cultivars. We investigated the underlying physiological, isotopic and morphological responses to water-deficit in seven common varieties of O. sativa, subjected to prolonged drought of varying intensities, for phenotyping purposes in open field conditions. Significant variation was observed in leaf-level photosynthesis rates (A) under both water treatments. Yield and A were influenced by the conductance of the mesophyll layer to CO2 (g m) and not by stomatal conductance (g s). Mesophyll conductance declined during drought to differing extents among the cultivars; those varieties that maintained g m during water-deficit sustained A and yield to a greater extent. However, the variety with the highest g m and yield under well-watered conditions (IR55419-04) was distinct from the most effective cultivar under drought (Vandana). Mesophyll conductance most effectively characterises the photosynthetic capacity and yield of O. sativa cultivars under both well-watered and water-deficit conditions; however, the desired attributes of high g m during optimal growth conditions and the capacity for g m to remain constant during water-deficit may be mutually exclusive. Nonetheless, future genetic and physiological studies aimed at enhancing O. sativa yield and drought stress tolerance should investigate the biochemistry and morphology of the interface between the sub-stomatal pore and mesophyll layer. PMID:25275452

  19. Whey cheese: membrane technology to increase yields.

    PubMed

    Riera, Francisco; González, Pablo; Muro, Claudia

    2016-02-01

    Sweet cheese whey has been used to obtain whey cheese without the addition of milk. Pre-treated whey was concentrated by nanofiltration (NF) at different concentration ratios (2, 2.5 and 2.8) or by reverse osmosis (RO) (2-3 times). After the concentration, whey was acidified with lactic acid until a final pH of 4.6-4.8, and heated to temperatures between 85 and 90 °C. The coagulated fraction (supernatant) was collected and freely drained over 4 h. The cheese-whey yield and protein, fat, lactose and ash recoveries in the final product were calculated. The membrane pre-concentration step caused an increase in the whey-cheese yield. The final composition of products was compared with traditional cheese-whey manufacture products (without membrane concentration). Final cheese yields found were to be between 5 and 19.6%, which are higher than those achieved using the traditional 'Requesón' process.

  20. Anomalous temperature dependence of yield stress and work hardening coefficient of B2-stabilized NiTi alloys

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hosoda, Hideki; Mishima, Yoshinao; Suzuki, Tomoo

    Yield stress and work hardening coefficient of B2-stabilized NiTi alloys are investigated using compression tests. Compositions of NiTi alloys are based on Ni-49mol.%Ti, to which Cr, Co and Al are chosen as ternary elements which reduce martensitic transformation temperatures of the B2 phase. Mechanical tests are carried out in liquid nitrogen at 77 K, air at room temperature (R.T.) and in an argon atmosphere between 473 K and 873 K. Only at 77 K, some alloys show characteristic stress-strain curves which indicate stress induced martensitic transformation (SIMT), but the others do not. Work hardening coefficient is found to be betweenmore » 2 and 11GPa in all the test temperature range. The values are extremely high compared with Young`s modulus of B2 NiTi. Yield stress and work hardening coefficient increase with test temperature between R.T. and about 650 K in most alloys. The anomalous temperature dependence of mechanical properties is not related to SIMT but to precipitation hardening and/or anomalous dislocation motion similar to B2-type CoTi. Solution hardening by adding ternary elements is evaluated to be small for Cr and Co additions, and large for Al addition, depending on difference in atomic size of the ternary element with respect to Ni or Ti.« less

  1. Influence of Yield Stress Determination in Anisotropic Hardening Model on Springback Prediction in Dual-Phase Steel

    NASA Astrophysics Data System (ADS)

    Lee, J.; Bong, H. J.; Ha, J.; Choi, J.; Barlat, F.; Lee, M.-G.

    2018-05-01

    In this study, a numerical sensitivity analysis of the springback prediction was performed using advanced strain hardening models. In particular, the springback in U-draw bending for dual-phase 780 steel sheets was investigated while focusing on the effect of the initial yield stress determined from the cyclic loading tests. The anisotropic hardening models could reproduce the flow stress behavior under the non-proportional loading condition for the considered parametric cases. However, various identification schemes for determining the yield stress of the anisotropic hardening models significantly influenced the springback prediction. The deviations from the measured springback varied from 4% to 13.5% depending on the identification method.

  2. Differential adaptation of two varieties of common bean to abiotic stress: I. Effects of drought on yield and photosynthesis.

    PubMed

    Lizana, Carolina; Wentworth, Mark; Martinez, Juan P; Villegas, Daniel; Meneses, Rodrigo; Murchie, Erik H; Pastenes, Claudio; Lercari, Bartolomeo; Vernieri, Paulo; Horton, Peter; Pinto, Manuel

    2006-01-01

    The yield of 24 commercial varieties and accessions of common bean (Phaseolus vulgaris) has been determined at different sites in Chile and Bolivia. Statistical analysis was performed in order to characterize whether a particular variety was more or less stable in yield under different environmental conditions. Amongst these, two varieties have been identified for more detailed study: one variety has a higher than average yield under unstressed conditions but is strongly affected by stress, and another has a reduced yield under unstressed conditions but is less affected by stress. The contrasting rate of abscission of the reproductive organs under drought stress was clearly consistent with these differences. The more tolerant genotype shows a great deal of plasticity at the biochemical and cellular level when exposed to drought stress, in terms of stomatal conductance, photosynthetic rate, abscisic acid synthesis, and resistance to photoinhibition. By contrast, the former lacks such plasticity, but shows an enhanced tendency for a morphological response, the movement of leaves, which appears to be its principal response to drought stress.

  3. Supportability of a High-Yield-Stress Slurry in a New Stereolithography-Based Ceramic Fabrication Process

    NASA Astrophysics Data System (ADS)

    He, Li; Song, Xuan

    2018-03-01

    In recent years, ceramic fabrication using stereolithography (SLA) has gained in popularity because of its high accuracy and density that can be achieved in the final part of production. One of the key challenges in ceramic SLA is that support structures are required for building overhanging features, whereas removing these support structures without damaging the components is difficult. In this research, a suspension-enclosing projection-stereolithography process is developed to overcome this challenge. This process uses a high-yield-stress ceramic slurry as the feedstock material and exploits the elastic force of the material to support overhanging features without the need for building additional support structures. Ceramic slurries with different solid loadings are studied to identify the rheological properties most suitable for supporting overhanging features. An analytical model of a double doctor-blade module is established to obtain uniform and thin recoating layers from a high-yield-stress slurry. Several test cases highlight the feasibility of using a high-yield-stress slurry to support overhanging features in SLA.

  4. Boiling of an emulsion in a yield stress fluid.

    PubMed

    Guéna, Geoffroy; Wang, Ji; d'Espinose, Jean-Baptiste; Lequeux, François; Talini, Laurence

    2010-11-01

    We report the boiling behavior of pentane emulsified in a yield stress fluid, a colloidal clay (Laponite) suspension. We have observed that a superheated state is easily reached: the emulsion, heated more than 50 °C above the alkane boiling point, does not boil. Superheating is made possible by the suppression of heterogeneous nucleation in pentane, resulting from the emulsification process, a phenomenon evidenced decades ago in studies of the superheating of two phase fluids. We have furthermore studied the growth of isolated bubbles nucleated in the emulsion. The rate of increase of the bubble radius with time depends on both the temperature and emulsion volume fraction but, rather unexpectedly, does not depend on the fluid rheology. We show that the bubbles grow by diffusion of the alkane through the aqueous phase between liquid droplets and bubbles, analogously to an Ostwald ripening process. The peculiarity of the process reported here is that a layer depleted in oil droplets forms around the bubble, layer to which the alkane concentration gradient is confined. We successfully describe our experimental results with a simple transfer model.

  5. Ants and termites increase crop yield in a dry climate.

    PubMed

    Evans, Theodore A; Dawes, Tracy Z; Ward, Philip R; Lo, Nathan

    2011-03-29

    Agricultural intensification has increased crop yields, but at high economic and environmental cost. Harnessing ecosystem services of naturally occurring organisms is a cheaper but under-appreciated approach, because the functional roles of organisms are not linked to crop yields, especially outside the northern temperate zone. Ecosystem services in soil come from earthworms in these cooler and wetter latitudes; what may fulfill their functional role in agriculture in warmer and drier habitats, where they are absent, is unproven. Here we show in a field experiment that ants and termites increase wheat yield by 36% from increased soil water infiltration due to their tunnels and improved soil nitrogen. Our results suggest that ants and termites have similar functional roles to earthworms, and that they may provide valuable ecosystem services in dryland agriculture, which may become increasingly important for agricultural sustainability in arid climates.

  6. Ants and termites increase crop yield in a dry climate

    PubMed Central

    Evans, Theodore A.; Dawes, Tracy Z.; Ward, Philip R.; Lo, Nathan

    2011-01-01

    Agricultural intensification has increased crop yields, but at high economic and environmental cost. Harnessing ecosystem services of naturally occurring organisms is a cheaper but under-appreciated approach, because the functional roles of organisms are not linked to crop yields, especially outside the northern temperate zone. Ecosystem services in soil come from earthworms in these cooler and wetter latitudes; what may fulfill their functional role in agriculture in warmer and drier habitats, where they are absent, is unproven. Here we show in a field experiment that ants and termites increase wheat yield by 36% from increased soil water infiltration due to their tunnels and improved soil nitrogen. Our results suggest that ants and termites have similar functional roles to earthworms, and that they may provide valuable ecosystem services in dryland agriculture, which may become increasingly important for agricultural sustainability in arid climates. PMID:21448161

  7. Electrorheological fluid with an extraordinarily high yield stress

    NASA Astrophysics Data System (ADS)

    Zhang, Yuling; Lu, Kunquan; Rao, Guanghui; Tian, Yu; Zhang, Shaohua; Liang, Jingkui

    2002-02-01

    Surface modified complex strontium titanate microparticles are synthesized by means of a modified sol-gel technique. A suspension composed of these particles immersed in a silicone oil exhibits excellent electrorheological properties attractive to industry and technology applications: a yield stress as high as 27 kPa in an applied electric field of 3 kV/mm, a low leakage current, wide dynamic ranges in temperature and shear rate, and a long-term stability against sedimentation. In addition to the high dielectric constant of strontium titanate, surfactant and water-free character of the particles may be responsible for the dramatic improvement of the electrorheological properties of the suspension.

  8. How can we improve crop genotypes to increase stress resilience and productivity in a future climate? A new crop screening method based on productivity and resistance to abiotic stress

    PubMed Central

    Thiry, Arnauld A.; Chavez Dulanto, Perla N.; Reynolds, Matthew P.; Davies, William J.

    2016-01-01

    The need to accelerate the selection of crop genotypes that are both resistant to and productive under abiotic stress is enhanced by global warming and the increase in demand for food by a growing world population. In this paper, we propose a new method for evaluation of wheat genotypes in terms of their resilience to stress and their production capacity. The method quantifies the components of a new index related to yield under abiotic stress based on previously developed stress indices, namely the stress susceptibility index, the stress tolerance index, the mean production index, the geometric mean production index, and the tolerance index, which were created originally to evaluate drought adaptation. The method, based on a scoring scale, offers simple and easy visualization and identification of resilient, productive and/or contrasting genotypes according to grain yield. This new selection method could help breeders and researchers by defining clear and strong criteria to identify genotypes with high resilience and high productivity and provide a clear visualization of contrasts in terms of grain yield production under stress. It is also expected that this methodology will reduce the time required for first selection and the number of first-selected genotypes for further evaluation by breeders and provide a basis for appropriate comparisons of genotypes that would help reveal the biology behind high stress productivity of crops. PMID:27677299

  9. Do increases in cigarette prices lead to increases in sales of cigarettes with high tar and nicotine yields?

    PubMed

    Farrelly, Matthew C; Loomis, Brett R; Mann, Nathan H

    2007-10-01

    We used scanner data on cigarette prices and sales collected from supermarkets across the United States from 1994 to 2004 to test the hypothesis that cigarette prices are positively correlated with sales of cigarettes with higher tar and nicotine content. During this period the average inflation-adjusted price for menthol cigarettes increased 55.8%. Price elasticities from multivariate regression models suggest that this price increase led to an increase of 1.73% in sales-weighted average tar yields and a 1.28% increase in sales-weighted average nicotine yields for menthol cigarettes. The 50.5% price increase of nonmenthol varieties over the same period yielded an estimated increase of 1% in tar per cigarette but no statistically significant increase in nicotine yields. An ordered probit model of the impact of cigarette prices on cigarette strength (ultra-light, light, full flavor, unfiltered) offers an explanation: As cigarette prices increase, the probability that stronger cigarette types will be sold increases. This effect is larger for menthol than for nonmenthol cigarettes. Our results are consistent with earlier population-based cross-sectional and longitudinal studies showing that higher cigarette prices and taxes are associated with increasing consumption of higher-yield cigarettes by smokers.

  10. Compressive yield stress of depletion gels from stationary centrifugation profiles

    NASA Astrophysics Data System (ADS)

    Lattuada, Enrico; Buzzaccaro, Stefano; Piazza, Roberto

    2018-01-01

    We have investigated the stationary sedimentation profiles of colloidal gels obtained by an arrested phase-separation process driven by depletion forces, which have been compressed either by natural gravity or by a centrifugal acceleration ranging between 6g and 2300g. Our measurements show that the gel rheological properties display a drastic change when the gel particle volume fraction exceeds a value φc , which barely depends on the strength of the interparticle attractive forces that consolidate the network. In particular, the gel compressive yield stress \\Pi(φ) , which increases as \\Pi(φ) ∼ φ4.2 for φ ≲ φc , displays a diverging behaviour for φ>φc , with an asymptotic value that is close to the random close packing value for hard spheres. The evidence we obtained suggests that φc basically coincides with the liquid (colloid-rich) branch of the metastable coexistence curve, rather than with the lower (and ϕ-dependent) values expected for an attractive glass line penetrating inside the coexistence region.

  11. Ncl Synchronously Regulates Na+, K+, and Cl- in Soybean and Greatly Increases the Grain Yield in Saline Field Conditions.

    PubMed

    Do, Tuyen Duc; Chen, Huatao; Hien, Vu Thi Thu; Hamwieh, Aladdin; Yamada, Tetsuya; Sato, Tadashi; Yan, Yongliang; Cong, Hua; Shono, Mariko; Suenaga, Kazuhiro; Xu, Donghe

    2016-01-08

    Salt stress inhibits soybean growth and reduces gain yield. Genetic improvement of salt tolerance is essential for sustainable soybean production in saline areas. In this study, we isolated a gene (Ncl) that could synchronously regulate the transport and accumulation of Na(+), K(+), and Cl(-) from a Brazilian soybean cultivar FT-Abyara using map-based cloning strategy. Higher expression of the salt tolerance gene Ncl in the root resulted in lower accumulations of Na(+), K(+), and Cl(-) in the shoot under salt stress. Transfer of Ncl with the Agrobacterium-mediated transformation method into a soybean cultivar Kariyutaka significantly enhanced its salt tolerance. Introgression of the tolerance allele into soybean cultivar Jackson, using DNA marker-assisted selection (MAS), produced an improved salt tolerance line. Ncl could increase soybean grain yield by 3.6-5.5 times in saline field conditions. Using Ncl in soybean breeding through gene transfer or MAS would contribute to sustainable soybean production in saline-prone areas.

  12. β-Aminobutyric acid increases abscisic acid accumulation and desiccation tolerance and decreases water use but fails to improve grain yield in two spring wheat cultivars under soil drying.

    PubMed

    Du, Yan-Lei; Wang, Zhen-Yu; Fan, Jing-Wei; Turner, Neil C; Wang, Tao; Li, Feng-Min

    2012-08-01

    A pot experiment was conducted to investigate the effect of the non-protein amino acid, β-aminobutyric acid (BABA), on the homeostasis between reactive oxygen species (ROS) and antioxidant defence during progressive soil drying, and its relationship with the accumulation of abscisic acid (ABA), water use, grain yield, and desiccation tolerance in two spring wheat (Triticum aestivum L.) cultivars released in different decades and with different yields under drought. Drenching the soil with 100 µM BABA increased drought-induced ABA production, leading to a decrease in the lethal leaf water potential (Ψ) used to measure desiccation tolerance, decreased water use, and increased water use efficiency for grain (WUEG) under moderate water stress. In addition, at severe water stress levels, drenching the soil with BABA reduced ROS production, increased antioxidant enzyme activity, and reduced the oxidative damage to lipid membranes. The data suggest that the addition of BABA triggers ABA accumulation that acts as a non-hydraulic root signal, thereby closing stomata, and reducing water use at moderate stress levels, and also reduces the production of ROS and increases the antioxidant defence enzymes at severe stress levels, thus increasing the desiccation tolerance. However, BABA treatment had no effect on grain yield of wheat when water availability was limited. The results suggest that there are ways of effectively priming the pre-existing defence pathways, in addition to genetic means, to improve the desiccation tolerance and WUEG of wheat.

  13. Theory of nonlinear elasticity, stress-induced relaxation, and dynamic yielding in dense fluids of hard nonspherical colloids

    NASA Astrophysics Data System (ADS)

    Zhang, Rui; Schweizer, Kenneth S.

    2012-04-01

    We generalize the microscopic naïve mode coupling and nonlinear Langevin equation theories of the coupled translation-rotation dynamics of dense suspensions of uniaxial colloids to treat the effect of applied stress on shear elasticity, cooperative cage escape, structural relaxation, and dynamic and static yielding. The key concept is a stress-dependent dynamic free energy surface that quantifies the center-of-mass force and torque on a moving colloid. The consequences of variable particle aspect ratio and volume fraction, and the role of plastic versus double glasses, are established in the context of dense, glass-forming suspensions of hard-core dicolloids. For low aspect ratios, the theory provides a microscopic basis for the recently observed phenomenon of double yielding as a consequence of stress-driven sequential unlocking of caging constraints via reduction of the distinct entropic barriers associated with the rotational and translational degrees of freedom. The existence, and breadth in volume fraction, of the double yielding phenomena is predicted to generally depend on both the degree of particle anisotropy and experimental probing frequency, and as a consequence typically occurs only over a window of (high) volume fractions where there is strong decoupling of rotational and translational activated relaxation. At high enough concentrations, a return to single yielding is predicted. For large aspect ratio dicolloids, rotation and translation are always strongly coupled in the activated barrier hopping event, and hence for all stresses only a single yielding process is predicted.

  14. Estimation of genetic parameters for heat stress, including dominance gene effects, on milk yield in Thai Holstein dairy cattle.

    PubMed

    Boonkum, Wuttigrai; Duangjinda, Monchai

    2015-03-01

    Heat stress in tropical regions is a major cause that strongly negatively affects to milk production in dairy cattle. Genetic selection for dairy heat tolerance is powerful technique to improve genetic performance. Therefore, the current study aimed to estimate genetic parameters and investigate the threshold point of heat stress for milk yield. Data included 52 701 test-day milk yield records for the first parity from 6247 Thai Holstein dairy cattle, covering the period 1990 to 2007. The random regression test day model with EM-REML was used to estimate variance components, genetic parameters and milk production loss. A decline in milk production was found when temperature and humidity index (THI) exceeded a threshold of 74, also it was associated with the high percentage of Holstein genetics. All variance component estimates increased with THI. The estimate of heritability of test-day milk yield was 0.231. Dominance variance as a proportion to additive variance (0.035) indicated that non-additive effects might not be of concern for milk genetics studies in Thai Holstein cattle. Correlations between genetic and permanent environmental effects, for regular conditions and due to heat stress, were - 0.223 and - 0.521, respectively. The heritability and genetic correlations from this study show that simultaneous selection for milk production and heat tolerance is possible. © 2014 Japanese Society of Animal Science.

  15. Yield Stress of Concentrated Zirconia Suspensions: Correlation with Particle Interactions.

    PubMed

    Megías-Alguacil; Durán; Delgado

    2000-11-01

    The presence of a sufficient concentration of solid particles in a solution gives rise to a large increase in its viscosity and, more importantly, to significant deviations with respect to its original Newtonian behavior. Different rheological techniques are available to characterize such deviations, but the simplest one, obtention of steady-state rheograms, is already extremely useful with that purpose. In this work, this technique is applied to suspensions of zirconia particles, both synthesized with spherical geometry and commercial. The sigma(shear stress)-gamma;(shear rate) curves show that the suspensions are nonideal plastic, thus exhibiting a finite yield stress, sigma(0), and a shear-thinning flow. It is through sigma(0) that a connection can be established between steady-state rheological behavior and interaction energy between particles, since sigma(0) can be estimated as the maximum attractive force between particles multiplied by the number of bonds per unit area between a given particle and its neighbors. Having an experimental determination of sigma(0), the verification of its relation with the attractive forces requires estimation of the potential energy of interaction between any pair of particles. Two approaches will be considered: one is the classical DLVO model, in which the potential energy, V, is the sum of the van der Waals (V(LW)) and electrostatic (V(EL)) contributions. The second approach is the so-called extended DLVO theory, in which the acid-base interaction V(AB) (related to the hydrophilic repulsion or hydrophobic attraction between the particles) is considered in addition to V(LW) and V(EL). The three contributions can be calculated as a function of the interparticle distance if the particle-solution interface is characterized from both the electric and the thermodynamic points of view. The former is carried out by means of electrophoretic mobility measurements and the latter by contact angle determinations for three probe liquids on

  16. Irrigation offsets wheat yield reductions from warming temperatures

    NASA Astrophysics Data System (ADS)

    Tack, Jesse; Barkley, Andrew; Hendricks, Nathan

    2017-11-01

    Temperature increases due to climate change are expected to cause substantial reductions in global wheat yields. However, uncertainty remains regarding the potential role for irrigation as an adaptation strategy to offset heat impacts. Here we utilize over 7000 observations spanning eleven Kansas field-trial locations, 180 varieties, and 29 years to show that irrigation significantly reduces the negative impact of warming temperatures on winter wheat yields. Dryland wheat yields are estimated to decrease about eight percent for every one-degree Celsius increase in temperature, yet irrigation completely offsets this negative impact in our sample. As in previous studies, we find that important interactions exist between heat stress and precipitation for dryland production. Here, uniquely, we observe both dryland and irrigated trials side-by-side at the same locations and find that precipitation does not provide the same reduction in heat stress as irrigation. This is likely to be because the timing, intensity, and volume of water applications influence wheat yields, so the ability to irrigate—rather than relying on rainfall alone—has a stronger influence on heat stress. We find evidence of extensive differences of water-deficit stress impacts across varieties. This provides some evidence of the potential for adapting to hotter and drier climate conditions using optimal variety selection. Overall, our results highlight the critical role of water management for future global food security. Water scarcity not only reduces crop yields through water-deficit stress, but also amplifies the negative effects of warming temperatures.

  17. Yield gap analyses to estimate attainable bovine milk yields and evaluate options to increase production in Ethiopia and India.

    PubMed

    Mayberry, Dianne; Ash, Andrew; Prestwidge, Di; Godde, Cécile M; Henderson, Ben; Duncan, Alan; Blummel, Michael; Ramana Reddy, Y; Herrero, Mario

    2017-07-01

    Livestock provides an important source of income and nourishment for around one billion rural households worldwide. Demand for livestock food products is increasing, especially in developing countries, and there are opportunities to increase production to meet local demand and increase farm incomes. Estimating the scale of livestock yield gaps and better understanding factors limiting current production will help to define the technological and investment needs in each livestock sector. The aim of this paper is to quantify livestock yield gaps and evaluate opportunities to increase dairy production in Sub-Saharan Africa and South Asia, using case studies from Ethiopia and India. We combined three different methods in our approach. Benchmarking and a frontier analysis were used to estimate attainable milk yields based on survey data. Household modelling was then used to simulate the effects of various interventions on dairy production and income. We tested interventions based on improved livestock nutrition and genetics in the extensive lowland grazing zone and highland mixed crop-livestock zones of Ethiopia, and the intensive irrigated and rainfed zones of India. Our analyses indicate that there are considerable yield gaps for dairy production in both countries, and opportunities to increase production using the interventions tested. In some cases, combined interventions could increase production past currently attainable livestock yields.

  18. [Increasing oxidative stress in aging].

    PubMed

    Shimosawa, Tatsuo

    2005-06-01

    The balance between reactive oxigen species (ROS) production and degradation is important in defining oxidative stress. In aging process, ROS production increases and degradation is impaired and thus oxidative stress is accumulated. Oxidative stress damages organs both directly and indirectly. Protein, lipid, as well as DNA are directly react with ROS, more over, ROS interact with intracellular signaling system. It is reported that several transcription factors such as NF-kappaB, AP-1 and ASK-1 and also it interferes MAPK activity. Besides these signaling, we recently showed that insulin resistance is induced by accumulated oxidative stress in aged mice. Adrenomedullin deficient mice accumulate higher oxidative stress and insulin resistance developed in aging. Oxidative stress in aging relates not only direct organ damage but also induce risk factors for vascular damage such as metabolic syndrome.

  19. Reduced yield stress for zirconium exposed to iodine: Reactive force field simulation

    DOE PAGES

    Rossi, Matthew L.; Taylor, Christopher D.; van Duin, Adri C. T.

    2014-11-04

    Iodine-induced stress-corrosion cracking (ISCC), a known failure mode for nuclear fuel cladding, occurs when iodine generated during the irradiation of a nuclear fuel pellet escapes the pellet through diffusion or thermal cracking and chemically interacts with the inner surface of the clad material, inducing a subsequent effect on the cladding’s resistance to mechanical stress. To complement experimental investigations of ISCC, a reactive force field (ReaxFF) compatible with the Zr-I chemical and materials systems has been developed and applied to simulate the impact of iodine exposure on the mechanical strength of the material. The study shows that the material’s resistance tomore » stress (as captured by the yield stress of a high-energy grain boundary) is related to the surface coverage of iodine, with the implication that ISCC is the result of adsorption-enhanced decohesion.« less

  20. Selecting Native Arbuscular Mycorrhizal Fungi to Promote Cassava Growth and Increase Yield under Field Conditions

    PubMed Central

    Séry, D. Jean-Marc; Kouadjo, Z. G. Claude; Voko, B. R. Rodrigue; Zézé, Adolphe

    2016-01-01

    The use of arbuscular mycorrhizal fungal (AMF) inoculation in sustainable agriculture is now widespread worldwide. Although the use of inoculants consisting of native AMF is highly recommended as an alternative to commercial ones, there is no strategy to allow the selection of efficient fungal species from natural communities. The objective of this study was (i) to select efficient native AMF species (ii) evaluate their impact on nematode and water stresses, and (iii) evaluate their impact on cassava yield, an important food security crop in tropical and subtropical regions. Firstly, native AMF communities associated with cassava rhizospheres in fields were collected from different areas and 7 AMF species were selected, based upon their ubiquity and abundance. Using these criteria, two morphotypes (LBVM01 and LBVM02) out of the seven AMF species selected were persistently dominant when cassava was used as a trap plant. LBVM01 and LBVM02 were identified as Acaulospora colombiana (most abundant) and Ambispora appendicula, respectively, after phylogenetic analyses of LSU-ITS-SSU PCR amplified products. Secondly, the potential of these two native AMF species to promote growth and enhance tolerance to root-knot nematode and water stresses of cassava (Yavo variety) was evaluated using single and dual inoculation in greenhouse conditions. Of the two AMF species, it was shown that A. colombiana significantly improved the growth of the cassava and enhanced tolerance to water stress. However, both A. colombiana and A. appendicula conferred bioprotective effects to cassava plants against the nematode Meloidogyne spp., ranging from resistance (suppression or reduction of the nematode reproduction) or tolerance (low or no suppression in cassava growth). Thirdly, the potential of these selected native AMF to improve cassava growth and yield was evaluated under field conditions, compared to a commercial inoculant. In these conditions, the A. colombiana single inoculation and the

  1. Effects of process variables on the yield stress of rheologically modified biomass

    Treesearch

    Joseph R. Samaniuk; C Tim Scott; Thatcher W. Root; Daniel J. Klingenberg

    2015-01-01

    Additives that alter the rheology of lignocellulosic biomass suspensions were tested under conditions of variable pH, temperature, and solid concentration. The effects of certain ions, biomass type, after the addition of rheological modifier were also examined. Torque and vane rheometry were used to measure the yield stress of samples. It was found that the...

  2. Combining Earthquake Focal Mechanism Inversion and Coulomb Friction Law to Yield Tectonic Stress Magnitudes in Strike-slip Faulting Regime

    NASA Astrophysics Data System (ADS)

    Soh, I.; Chang, C.

    2017-12-01

    The techniques for estimating present-day stress states by inverting multiple earthquake focal mechanism solutions (FMS) provide orientations of the three principal stresses and their relative magnitudes. In order to estimate absolute magnitudes of the stresses that are generally required to analyze faulting mechanics, we combine the relative stress magnitude parameter (R-value) derived from the inversion process and the concept of frictional equilibrium of stress state defined by Coulomb friction law. The stress inversion in Korean Peninsula using 152 FMS data (magnitude≥2.5) conducted at regularly spaced grid points yields a consistent strike-slip faulting regime in which the maximum (S1) and the minimum (S3) principal stresses act in horizontal planes (with an S1 azimuth in ENE-WSW) and the intermediate principal stress (S2) close to vertical. However, R-value varies from 0.28 to 0.75 depending on locations, systematically increasing eastward. Based on the assumptions that the vertical stress is lithostatic, pore pressure is hydrostatic, and the maximum differential stress (S1-S3) is limited by Byerlee's friction of optimally oriented faults for slip, we estimate absolute magnitudes of the two horizontal principal stresses using R-value. As R-value increases, so do the magnitudes of the horizontal stresses. Our estimation of the stress magnitudes shows that the maximum horizontal principal stress (S1) normalized by vertical stress tends to increase from 1.3 in the west to 1.8 in the east. The estimated variation of stress magnitudes is compatible with distinct clustering of faulting types in different regions. Normal faulting events are densely populated in the west region where the horizontal stress is relatively low, whereas numerous reverse faulting events prevail in the east offshore where the horizontal stress is relatively high. Such a characteristic distribution of distinct faulting types in different regions can only be explained in terms of stress

  3. Kinetics model of bainitic transformation with stress

    NASA Astrophysics Data System (ADS)

    Zhou, Mingxing; Xu, Guang; Hu, Haijiang; Yuan, Qing; Tian, Junyu

    2018-01-01

    Thermal simulations were conducted on a Gleeble 3800 simulator. The main purpose is to investigate the effects of stress on the kinetics of bainitic transformation in a Fe-C-Mn-Si advanced high strength bainitic steel. Previous studies on modeling the kinetics of stress affected bainitic transformation only considered the stress below the yield strength of prior austenite. In the present study, the stress above the yield strength of prior austenite is taken into account. A new kinetics model of bainitic transformation dependent on the stress (including the stresses below and above the yield strength of prior austenite) and the transformation temperature is proposed. The new model presents a good agreement with experimental results. In addition, it is found that the acceleration degree of stress on bainitic transformation increases with the stress whether its magnitude is below or above the yield strength of austenite, but the increasing rate gradually slows down when the stress is above the yield strength of austenite.

  4. Genome editing using CRISPR/Cas9-targeted mutagenesis: An opportunity for yield improvements of crop plants grown under environmental stresses.

    PubMed

    Abdelrahman, Mostafa; Al-Sadi, Abdullah M; Pour-Aboughadareh, Alireza; Burritt, David J; Tran, Lam-Son Phan

    2018-03-12

    Developing more crops able to sustainably produce high yields when grown under biotic/abiotic stresses is an important goal, if crop production and food security are to be guaranteed in the face of ever-increasing human population and unpredictable global climatic conditions. However, conventional crop improvement, through random mutagenesis or genetic recombination, is time-consuming and cannot keep pace with increasing food demands. Targeted genome editing (GE) technologies, especially clustered regularly interspaced short palindromic repeats (CRISPR)/(CRISPR)-associated protein 9 (Cas9), have great potential to aid in the breeding of crops that are able to produce high yields under conditions of biotic/abiotic stress. This is due to their high efficiency, accuracy and low risk of off-target effects, compared with conventional random mutagenesis methods. The use of CRISPR/Cas9 system has grown very rapidly in recent years with numerous examples of targeted mutagenesis in crop plants, including gene knockouts, modifications, and the activation and repression of target genes. The potential of the GE approach for crop improvement has been clearly demonstrated. However, the regulation and social acceptance of GE crops still remain a challenge. In this review, we evaluate the recent applications of the CRISPR/Cas9-mediated GE, as a means to produce crop plants with greater resilience to the stressors they encounter when grown under increasing stressful environmental conditions. Copyright © 2018 Elsevier Masson SAS. All rights reserved.

  5. Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field.

    PubMed

    Selvaraj, Michael Gomez; Ishizaki, Takuma; Valencia, Milton; Ogawa, Satoshi; Dedicova, Beata; Ogata, Takuya; Yoshiwara, Kyouko; Maruyama, Kyonoshin; Kusano, Miyako; Saito, Kazuki; Takahashi, Fuminori; Shinozaki, Kazuo; Nakashima, Kazuo; Ishitani, Manabu

    2017-11-01

    Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGolS2) was able to confer drought tolerance and increase grain yield in two different rice (Oryza sativa) genotypes under dry field conditions. The developed transgenic lines expressing AtGolS2 under the control of the constitutive maize ubiquitin promoter (Ubi:AtGolS2) also had higher levels of galactinol than the non-transgenic control. The increased grain yield of the transgenic rice under drought conditions was related to a higher number of panicles, grain fertility and biomass. Extensive confined field trials using Ubi:AtGolS2 transgenic lines in Curinga, tropical japonica and NERICA4, interspecific hybrid across two different seasons and environments revealed the verified lines have the proven field drought tolerance of the Ubi:AtGolS2 transgenic rice. The amended drought tolerance was associated with higher relative water content of leaves, higher photosynthesis activity, lesser reduction in plant growth and faster recovering ability. Collectively, our results provide strong evidence that AtGolS2 is a useful biotechnological tool to reduce grain yield losses in rice beyond genetic differences under field drought stress. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  6. Short communication: Estimation of yield stress/viscosity of molten octol

    DOE PAGES

    Davis, S. M.; Zerkle, D. K.

    2018-05-04

    Explosive HMX particles are similar in morphology and chemistry to RDX particles, the main constituent of Composition B-3 (Comp B-3). This suggests molten HMX-TNT formulations may show Bingham plasticity, much like recent studies have shown for Comp B-3. Here a Bingham plastic viscosity model, including yield stress and shear thinning, is presented for octol (70/30wt% HMX/TNT) as a function of HMX particle volume fraction. The effect of HMX dissolution into molten TNT is included in this analysis.

  7. Short communication: Estimation of yield stress/viscosity of molten octol

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Davis, S. M.; Zerkle, D. K.

    Explosive HMX particles are similar in morphology and chemistry to RDX particles, the main constituent of Composition B-3 (Comp B-3). This suggests molten HMX-TNT formulations may show Bingham plasticity, much like recent studies have shown for Comp B-3. Here a Bingham plastic viscosity model, including yield stress and shear thinning, is presented for octol (70/30wt% HMX/TNT) as a function of HMX particle volume fraction. The effect of HMX dissolution into molten TNT is included in this analysis.

  8. Short communication: Estimation of yield stress/viscosity of molten octol

    NASA Astrophysics Data System (ADS)

    Davis, S. M.; Zerkle, D. K.

    2018-05-01

    Explosive HMX particles are similar in morphology and chemistry to RDX particles, the main constituent of Composition B-3 (Comp B-3). This suggests molten HMX-TNT formulations may show Bingham plasticity, much like recent studies have shown for Comp B-3. Here a Bingham plastic viscosity model, including yield stress and shear thinning, is presented for octol (70/30wt% HMX/TNT) as a function of HMX particle volume fraction. The effect of HMX dissolution into molten TNT is included in this analysis.

  9. African crop yield reductions due to increasingly unbalanced Nitrogen and Phosphorus consumption

    NASA Astrophysics Data System (ADS)

    van der Velde, Marijn; Folberth, Christian; Balkovič, Juraj; Ciais, Philippe; Fritz, Steffen; Janssens, Ivan A.; Obersteiner, Michael; See, Linda; Skalský, Rastislav; Xiong, Wei; Peñuealas, Josep

    2014-05-01

    The impact of soil nutrient depletion on crop production has been known for decades, but robust assessments of the impact of increasingly unbalanced nitrogen (N) and phosphorus (P) application rates on crop production are lacking. Here, we use crop response functions based on 741 FAO maize crop trials and EPIC crop modeling across Africa to examine maize yield deficits resulting from unbalanced N:P applications under low, medium, and high input scenarios, for past (1975), current, and future N:P mass ratios of respectively, 1:0.29, 1:0.15, and 1:0.05. At low N inputs (10 kg/ha), current yield deficits amount to 10% but will increase up to 27% under the assumed future N:P ratio, while at medium N inputs (50 kg N/ha), future yield losses could amount to over 40%. The EPIC crop model was then used to simulate maize yields across Africa. The model results showed relative median future yield reductions at low N inputs of 40%, and 50% at medium and high inputs, albeit with large spatial variability. Dominant low-quality soils such as Ferralsols, which are strongly adsorbing P, and Arenosols with a low nutrient retention capacity, are associated with a strong yield decline, although Arenosols show very variable crop yield losses at low inputs. Optimal N:P ratios, i.e. those where the lowest amount of applied P produces the highest yield (given N input) where calculated with EPIC to be as low as 1:0.5. Finally, we estimated the additional P required given current N inputs, and given N inputs that would allow Africa to close yield gaps (ca. 70%). At current N inputs, P consumption would have to increase 2.3-fold to be optimal, and to increase 11.7-fold to close yield gaps. The P demand to overcome these yield deficits would provide a significant additional pressure on current global extraction of P resources.

  10. Increasing drought and diminishing benefits of elevated carbon dioxide for soybean yields across the US Midwest.

    PubMed

    Jin, Zhenong; Ainsworth, Elizabeth A; Leakey, Andrew D B; Lobell, David B

    2018-02-01

    Elevated atmospheric CO 2 concentrations ([CO 2 ]) are expected to increase C3 crop yield through the CO 2 fertilization effect (CFE) by stimulating photosynthesis and by reducing stomatal conductance and transpiration. The latter effect is widely believed to lead to greater benefits in dry rather than wet conditions, although some recent experimental evidence challenges this view. Here we used a process-based crop model, the Agricultural Production Systems sIMulator (APSIM), to quantify the contemporary and future CFE on soybean in one of its primary production area of the US Midwest. APSIM accurately reproduced experimental data from the Soybean Free-Air CO 2 Enrichment site showing that the CFE declined with increasing drought stress. This resulted from greater radiation use efficiency (RUE) and above-ground biomass production at elevated [CO 2 ] that outpaced gains in transpiration efficiency (TE). Using an ensemble of eight climate model projections, we found that drought frequency in the US Midwest is projected to increase from once every 5 years currently to once every other year by 2050. In addition to directly driving yield loss, greater drought also significantly limited the benefit from rising [CO 2 ]. This study provides a link between localized experiments and regional-scale modeling to highlight that increased drought frequency and severity pose a formidable challenge to maintaining soybean yield progress that is not offset by rising [CO 2 ] as previously anticipated. Evaluating the relative sensitivity of RUE and TE to elevated [CO 2 ] will be an important target for future modeling and experimental studies of climate change impacts and adaptation in C3 crops. © 2017 John Wiley & Sons Ltd.

  11. Increasing crop yield and resilience with trehalose 6-phosphate: targeting a feast-famine mechanism in cereals for better source-sink optimization.

    PubMed

    Paul, Matthew J; Oszvald, Maria; Jesus, Claudia; Rajulu, Charukesi; Griffiths, Cara A

    2017-07-20

    Food security is a pressing global issue. New approaches are required to break through a yield ceiling that has developed in recent years for the major crops. As important as increasing yield potential is the protection of yield from abiotic stresses in an increasingly variable and unpredictable climate. Current strategies to improve yield include conventional breeding, marker-assisted breeding, quantitative trait loci (QTLs), mutagenesis, creation of hybrids, genetic modification (GM), emerging genome-editing technologies, and chemical approaches. A regulatory mechanism amenable to three of these approaches has great promise for large yield improvements. Trehalose 6-phosphate (T6P) synthesized in the low-flux trehalose biosynthetic pathway signals the availability of sucrose in plant cells as part of a whole-plant sucrose homeostatic mechanism. Modifying T6P content by GM, marker-assisted selection, and novel chemistry has improved yield in three major cereals under a range of water availabilities from severe drought through to flooding. Yield improvements have been achieved by altering carbon allocation and how carbon is used. Targeting T6P both temporally and spatially offers great promise for large yield improvements in productive (up to 20%) and marginal environments (up to 120%). This opinion paper highlights this important breakthrough in fundamental science for crop improvement. © The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  12. Experimental study of the solid-liquid interface in a yield-stress fluid flow upstream of a step

    NASA Astrophysics Data System (ADS)

    Luu, Li-Hua; Pierre, Philippe; Guillaume, Chambon

    2014-11-01

    We present an experimental study where a yield-stress fluid is implemented to carefully examine the interface between a liquid-like unyielded region and a solid-like yielded region. The studied hydrodynamics consists of a rectangular pipe-flow disturbed by the presence of a step. Upstream of the step, a solid-liquid interface between a dead zone and a flow zone appears. This configuration can both model geophysical erosion phenomenon in debris flows or find applications for industrial extrusion processes. We aim to investigate the dominant physical mechanism underlying the formation of the static domain, by combining the rheological characterization of the yield-stress fluid with local measurements of the related hydrodynamic parameters. In this work, we use a model fluid, namely polymer micro-gel Carbopol, that exhibits a Hershel-Bulkley viscoplastic rheology. Exploiting the fluid transparency, the flow is monitored by Particle Image Velocimetry thanks to internal visualization technique. In particular, we demonstrate that the flow above the dead zone roughly behaves as a plug flow whose velocity profile can successfully be described by a Poiseuille equation including a Hershel-Bulkley rheology (PHB theory), with exception of a thin zone at the close vicinity of the static domain. The border inside the flow zone above which the so-called PHB flow starts, is found to be the same regardless of the flow rate and to move with a constant velocity that increases with the flow rate. We interpret this feature as a slip frontier.

  13. Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

    PubMed Central

    Wei, Likun; Huang, Xuxiong

    2017-01-01

    Microalga Nannochloropsis oculata is a promising alternative feedstock for biodiesel. Elevating its oil-yielding capacity is conducive to cost-saving biodiesel production. However, the regulatory processes of multi-factor collaborative stresses (MFCS) on the oil-yielding performance of N. oculata are unclear. The duration effects of MFCS (high irradiation, nitrogen deficiency and elevated iron supplementation) on N. oculata were investigated in an 18-d batch culture. Despite the reduction in cell division, the biomass concentration increased, resulting from the large accumulation of the carbon/energy-reservoir. However, different storage forms were found in different cellular storage compounds, and both the protein content and pigment composition swiftly and drastically changed. The analysis of four biodiesel properties using pertinent empirical equations indicated their progressive effective improvement in lipid classes and fatty acid composition. The variation curve of neutral lipid productivity was monitored with fluorescent Nile red and was closely correlated to the results from conventional methods. In addition, a series of changes in the organelles (e.g., chloroplast, lipid body and vacuole) and cell shape, dependent on the stress duration, were observed by TEM and LSCM. These changes presumably played an important role in the acclimation of N. oculata to MFCS and accordingly improved its oil-yielding performance. PMID:28346505

  14. Role of aquaporins in determining transpiration and photosynthesis in water-stressed plants: crop water-use efficiency, growth and yield.

    PubMed

    Moshelion, Menachem; Halperin, Ofer; Wallach, Rony; Oren, Ram; Way, Danielle A

    2015-09-01

    The global shortage of fresh water is one of our most severe agricultural problems, leading to dry and saline lands that reduce plant growth and crop yield. Here we review recent work highlighting the molecular mechanisms allowing some plant species and genotypes to maintain productivity under water stress conditions, and suggest molecular modifications to equip plants for greater production in water-limited environments. Aquaporins (AQPs) are thought to be the main transporters of water, small and uncharged solutes, and CO2 through plant cell membranes, thus linking leaf CO2 uptake from the intercellular airspaces to the chloroplast with water loss pathways. AQPs appear to play a role in regulating dynamic changes of root, stem and leaf hydraulic conductivity, especially in response to environmental changes, opening the door to using AQP expression to regulate plant water-use efficiency. We highlight the role of vascular AQPs in regulating leaf hydraulic conductivity and raise questions regarding their role (as well as tonoplast AQPs) in determining the plant isohydric threshold, growth rate, fruit yield production and harvest index. The tissue- or cell-specific expression of AQPs is discussed as a tool to increase yield relative to control plants under both normal and water-stressed conditions. © 2014 John Wiley & Sons Ltd.

  15. Job stress and productivity increase.

    PubMed

    Adaramola, Samson Sunday

    2012-01-01

    This paper examines mental and physical pressures that workers bear at work. The authors discuss how on the-job stress affects a person's capabilities and productivity, and how such pressures lend to higher incidences of accidents at work. The paper also discuss methods of reducing job-related stress and increasing productivity. An intervention was conducted amongst workers in a private firm. It shows mental and emotional pressure can affect performance and productivity of a worker on the job. One of the biggest influences of today's worker is on the-job stress. Job stress occurs when the requirements of the job do not match the capabilities, resources, or needs of the worker. This consequently affects how a person would normally deal with customer service problems, grievances, violence, conflict, and decisions on the job. Stress is an inevitable part of everyday life, and is therefore a distinct part of a person's job. To properly control the outcome of stress, there are certain precautions and methods that should be taken that will boost productivity.

  16. Increased neural responses to empathy for pain might explain how acute stress increases prosociality

    PubMed Central

    Tomova, L.; Majdandžić, J.; Hummer, A.; Windischberger, C.; Heinrichs, M.

    2017-01-01

    Abstract Recent behavioral investigations suggest that acute stress can increase prosocial behavior. Here, we investigated whether increased empathy represents a potential mechanism for this finding. Using functional magnetic resonance imaging, we assessed the effects of acute stress on neural responses related to automatic and regulatory components of empathy for pain as well as subsequent prosocial behavior. Stress increased activation in brain areas associated with the automatic sharing of others’ pain, such as the anterior insula, the anterior midcingulate cortex, and the primary somatosensory cortex. In addition, we found increased prosocial behavior under stress. Furthermore, activation in the anterior midcingulate cortex mediated the effects of stress on prosocial behavior. However, stressed participants also displayed stronger and inappropriate other-related responses in situations which required them to take the perspective of another person, and to regulate their automatic affective responses. Thus, while acute stress may increase prosocial behavior by intensifying the sharing of others’ emotions, this comes at the cost of reduced cognitive appraisal abilities. Depending on the contextual constraints, stress may therefore affect empathy in ways that are either beneficial or detrimental. PMID:27798249

  17. Examining the roles that changing harvested areas, closing yield-gaps, and increasing yield ceilings have had on crop production

    NASA Astrophysics Data System (ADS)

    Johnston, M.; Ray, D. K.; Mueller, N. D.; Foley, J. A.

    2011-12-01

    With an increasing and increasingly affluent population, there has been tremendous effort to examine strategies for sustainably increasing agricultural production to meet this surging global demand. Before developing new solutions from scratch, though, we believe it is important to consult our recent agricultural history to see where and how agricultural production changes have already taken place. By utilizing the newly created temporal M3 cropland datasets, we can for the first time examine gridded agricultural yields and area, both spatially and temporally. This research explores the historical drivers of agricultural production changes, from 1965-2005. The results will be presented spatially at the global-level (5-min resolution), as well as at the individual country-level. The primary research components of this study are presented below, including the general methodology utilized in each phase and preliminary results for soybean where available. The complete assessment will cover maize, wheat, rice, soybean, and sugarcane, and will include country-specific analysis for over 200 countries, states, territories and protectorates. Phase 1: The first component of our research isolates changes in agricultural production due to variation in planting decisions (harvested area) from changes in production due to intensification efforts (yield). We examine area/yield changes at the pixel-level over 5-year time-steps to determine how much each component has contributed to overall changes in production. Our results include both spatial patterns of changes in production, as well as spatial maps illustrating to what degree the production change is attributed to area and/or yield. Together, these maps illustrate where, why, and by how much agricultural production has changed over time. Phase 2: In the second phase of our research we attempt to determine the impact that area and yield changes have had on agricultural production at the country-level. We calculate a production

  18. Future Warming Increases Global Maize Yield Variability with Implications for Food Markets

    NASA Astrophysics Data System (ADS)

    Tigchelaar, M.; Battisti, D. S.; Naylor, R. L.; Ray, D. K.

    2017-12-01

    If current trends in population growth and dietary shifts continue, the world will need to produce about 70% more food by 2050, while earth's climate is rapidly changing. Rising temperatures in particular are projected to negatively impact agricultural production, as the world's staple crops perform poorly in extreme heat. Theoretical models suggest that as temperatures rise above plants' optimal temperature for performance, not only will mean yields decline rapidly, but the variability of yields will increase, even as interannual variations in climate remain unchanged. Here we use global datasets of maize production and climate variability combined with CMIP5 temperature projections to quantify how yield variability will change in major maize producing countries under 2°C and 4°C of global warming. Maize is the world's most produced crop, and is linked to other staple crops through substitution in consumption and production. We find that in warmer climates - absent any breeding gains in heat tolerance - the Coefficient of Variation (CV) of maize yields increases almost everywhere, to values much larger than present-day. This increase in CV is due both to an increase in the standard deviation of yields, and a decrease in mean yields. In locations where crop failures become the norm under high (4°C) warming (mostly in tropical, low-yield environments), the standard deviation of yields ultimately decreases. The probability that in any given year the most productive areas in the top three maize producing countries (United States, China, Brazil) have simultaneous production losses greater than 10% is virtually zero under present-day climate conditions, but increases to 12% under 2°C warming, and 89% under 4°C warming. This has major implications for global food markets and staple crop prices, affecting especially the 2.5 billion people that comprise the world's poor, who already spend the majority of their disposable income on food and are particularly vulnerable

  19. Effective Use of Water and Increased Dry Matter Partitioned to Grain Contribute to Yield of Common Bean Improved for Drought Resistance

    PubMed Central

    Polania, Jose A.; Poschenrieder, Charlotte; Beebe, Stephen; Rao, Idupulapati M.

    2016-01-01

    Common bean (Phaseolus vulgaris L.) is the most important food legume in the diet of poor people in the tropics. Drought causes severe yield loss in this crop. Identification of traits associated with drought resistance contributes to improving the process of generating bean genotypes adapted to these conditions. Field studies were conducted at the International Center for Tropical Agriculture (CIAT), Palmira, Colombia, to determine the relationship between grain yield and different parameters such as effective use of water (EUW), canopy biomass, and dry partitioning indices (pod partitioning index, harvest index, and pod harvest index) in elite lines selected for drought resistance over the past decade. Carbon isotope discrimination (CID) was used for estimation of water use efficiency (WUE). The main objectives were: (i) to identify specific morpho-physiological traits that contribute to improved resistance to drought in lines developed over several cycles of breeding and that could be useful as selection criteria in breeding; and (ii) to identify genotypes with desirable traits that could serve as parents in the corresponding breeding programs. A set of 36 bean genotypes belonging to the Middle American gene pool were evaluated under field conditions with two levels of water supply (irrigated and drought) over two seasons. Eight bean lines (NCB 280, NCB 226, SEN 56, SCR 2, SCR 16, SMC 141, RCB 593, and BFS 67) were identified as resistant to drought stress. Resistance to terminal drought stress was positively associated with EUW combined with increased dry matter partitioned to pod and seed production and negatively associated with days to flowering and days to physiological maturity. Differences in genotypic response were observed between grain CID and grain yield under irrigated and drought stress. Based on phenotypic differences in CID, leaf stomatal conductance, canopy biomass, and grain yield under drought stress, the lines tested were classified into two

  20. A Benzimidazole Proton Pump Inhibitor Increases Growth and Tolerance to Salt Stress in Tomato.

    PubMed

    Van Oosten, Michael J; Silletti, Silvia; Guida, Gianpiero; Cirillo, Valerio; Di Stasio, Emilio; Carillo, Petronia; Woodrow, Pasqualina; Maggio, Albino; Raimondi, Giampaolo

    2017-01-01

    Pre-treatment of tomato plants with micromolar concentrations of omeprazole (OP), a benzimidazole proton pump inhibitor in mammalian systems, improves plant growth in terms of fresh weight of shoot and roots by 49 and 55% and dry weight by 54 and 105% under salt stress conditions (200 mM NaCl), respectively. Assessment of gas exchange, ion distribution, and gene expression profile in different organs strongly indicates that OP interferes with key components of the stress adaptation machinery, including hormonal control of root development (improving length and branching), protection of the photosynthetic system (improving quantum yield of photosystem II) and regulation of ion homeostasis (improving the K + :Na + ratio in leaves and roots). To our knowledge OP is one of the few known molecules that at micromolar concentrations manifests a dual function as growth enhancer and salt stress protectant. Therefore, OP can be used as new inducer of stress tolerance to better understand molecular and physiological stress adaptation paths in plants and to design new products to improve crop performance under suboptimal growth conditions. Highlight: Omeprazole enhances growth of tomato and increases tolerance to salinity stress through alterations of gene expression and ion uptake and transport.

  1. A Benzimidazole Proton Pump Inhibitor Increases Growth and Tolerance to Salt Stress in Tomato

    PubMed Central

    Van Oosten, Michael J.; Silletti, Silvia; Guida, Gianpiero; Cirillo, Valerio; Di Stasio, Emilio; Carillo, Petronia; Woodrow, Pasqualina; Maggio, Albino; Raimondi, Giampaolo

    2017-01-01

    Pre-treatment of tomato plants with micromolar concentrations of omeprazole (OP), a benzimidazole proton pump inhibitor in mammalian systems, improves plant growth in terms of fresh weight of shoot and roots by 49 and 55% and dry weight by 54 and 105% under salt stress conditions (200 mM NaCl), respectively. Assessment of gas exchange, ion distribution, and gene expression profile in different organs strongly indicates that OP interferes with key components of the stress adaptation machinery, including hormonal control of root development (improving length and branching), protection of the photosynthetic system (improving quantum yield of photosystem II) and regulation of ion homeostasis (improving the K+:Na+ ratio in leaves and roots). To our knowledge OP is one of the few known molecules that at micromolar concentrations manifests a dual function as growth enhancer and salt stress protectant. Therefore, OP can be used as new inducer of stress tolerance to better understand molecular and physiological stress adaptation paths in plants and to design new products to improve crop performance under suboptimal growth conditions. Highlight: Omeprazole enhances growth of tomato and increases tolerance to salinity stress through alterations of gene expression and ion uptake and transport. PMID:28769943

  2. Effect of Arbuscular Mycorrhizal Fungi On Yield and Phytoremediation Performance of Pot Marigold (Calendula officinalis L.) Under Heavy Metals Stress.

    PubMed

    Tabrizi, Leila; Mohammadi, Siavash; Delshad, Mojtaba; Moteshare Zadeh, Babak

    2015-01-01

    In order to study the effect of mycorrhizal fungi (inoculated and non-inoculated) and heavy metals stress [0, Pb (150 and 300 mg/kg) and Cd (40 and 80 mg/kg)] on pot marigold (Calendula officinalis L.), a factorial experiment was conducted based on a randomized complete block design with 4 replications in Research Greenhouse of Department of Horticultural Sciences, University of Tehran, Iran, during 2012-2013. Plant height, herbal and flower fresh and dry weight, root fresh and dry weight and root volume, colonization percentage, total petal extract, total petal flavonoids, root and shoot P and K uptakes, and Pb and Cd accumulations in root and shoot were measured. Results indicated that with increasing soil Pb and Cd concentration, growth and yield of pot marigold was reduced significantly; Cd had greater negative impacts than Pb. However, mycorrhizal fungi alleviated these impacts by improving plant growth and yield. Pot marigold concentrated high amounts of Pb and especially Cd in its roots and shoots; mycorrhizal plants had a greater accumulation of these metals, so that those under 80 mg/kg Cd soil(-1) accumulated 833.3 and 1585.8 mg Cd in their shoots and roots, respectively. In conclusion, mycorrhizal fungi can improve not only growth and yield of pot marigold in heavy metal stressed condition, but also phytoremediation performance by increasing heavy metals accumulation in the plant organs.

  3. Increased neural responses to empathy for pain might explain how acute stress increases prosociality.

    PubMed

    Tomova, L; Majdandžic, J; Hummer, A; Windischberger, C; Heinrichs, M; Lamm, C

    2017-03-01

    Recent behavioral investigations suggest that acute stress can increase prosocial behavior. Here, we investigated whether increased empathy represents a potential mechanism for this finding. Using functional magnetic resonance imaging, we assessed the effects of acute stress on neural responses related to automatic and regulatory components of empathy for pain as well as subsequent prosocial behavior. Stress increased activation in brain areas associated with the automatic sharing of others' pain, such as the anterior insula, the anterior midcingulate cortex, and the primary somatosensory cortex. In addition, we found increased prosocial behavior under stress. Furthermore, activation in the anterior midcingulate cortex mediated the effects of stress on prosocial behavior. However, stressed participants also displayed stronger and inappropriate other-related responses in situations which required them to take the perspective of another person, and to regulate their automatic affective responses. Thus, while acute stress may increase prosocial behavior by intensifying the sharing of others' emotions, this comes at the cost of reduced cognitive appraisal abilities. Depending on the contextual constraints, stress may therefore affect empathy in ways that are either beneficial or detrimental. © The Author (2016). Published by Oxford University Press.

  4. Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

    PubMed Central

    2011-01-01

    Background Pichia pastoris is a widely-used host for recombinant protein production. Initial screening for both suitable clones and optimum culture conditions is typically carried out in multi-well plates. This is followed by up-scaling either to shake-flasks or continuously stirred tank bioreactors. A particular problem in these formats is foaming, which is commonly prevented by the addition of chemical antifoaming agents. Intriguingly, antifoams are often added without prior consideration of their effect on the yeast cells, the protein product or the influence on downstream processes such as protein purification. In this study we characterised, for the first time, the effects of five commonly-used antifoaming agents on the total amount of recombinant green fluorescent protein (GFP) secreted from shake-flask cultures of this industrially-relevant yeast. Results Addition of defined concentrations of Antifoam A (Sigma), Antifoam C (Sigma), J673A (Struktol), P2000 (Fluka) or SB2121 (Struktol) to shake-flask cultures of P. pastoris increased the total amount of recombinant GFP in the culture medium (the total yield) and in the case of P2000, SB2121 and J673A almost doubled it. When normalized to the culture density, the GFP specific yield (μg OD595-1) was only increased for Antifoam A, Antifoam C and J673A. Whilst none of the antifoams affected the growth rate of the cells, addition of P2000 or SB2121 was found to increase culture density. There was no correlation between total yield, specific yield or specific growth rate and the volumetric oxygen mass transfer coefficient (kLa) in the presence of antifoam. Moreover, the antifoams did not affect the dissolved oxygen concentration of the cultures. A comparison of the amount of GFP retained in the cell by flow cytometry with that in the culture medium by fluorimetry suggested that addition of Antifoam A, Antifoam C or J673A increased the specific yield of GFP by increasing the proportion secreted into the medium

  5. Overexpression of OsCYP19-4 increases tolerance to cold stress and enhances grain yield in rice (Oryza sativa).

    PubMed

    Yoon, Dae Hwa; Lee, Sang Sook; Park, Hyun Ji; Lyu, Jae Il; Chong, Won Seog; Liu, Jang Ryol; Kim, Beom-Gi; Ahn, Jun Cheul; Cho, Hye Sun

    2016-01-01

    AtCYP19-4 (also known as CYP5) was previously identified as interacting in vitro with GNOM, a member of a large family of ARF guanine nucleotide exchange factors that is required for proper polar localization of the auxin efflux carrier PIN1. The present study demonstrated that OsCYP19-4, a gene encoding a putative homologue of AtCYP19-4, was up-regulated by several stresses and showed over 10-fold up-regulation in response to cold. The study further demonstrated that the promoter of OsCYP19-4 was activated in response to cold stress. An OsCYP19-4-GFP fusion protein was targeted to the outside of the plasma membrane via the endoplasmic reticulum as determined using brefeldin A, a vesicle trafficking inhibitor. An in vitro assay with a synthetic substrate oligomer confirmed that OsCYP19-4 had peptidyl-prolyl cis-trans isomerase activity, as was previously reported for AtCYP19-4. Rice plants overexpressing OsCYP19-4 showed cold-resistance phenotypes with significantly increased tiller and spike numbers, and consequently enhanced grain weight, compared with wild-type plants. Based on these results, the authors suggest that OsCYP19-4 is required for developmental acclimation to environmental stresses, especially cold. Furthermore, the results point to the potential of manipulating OsCYP19-4 expression to enhance cold tolerance or to increase biomass. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  6. The yield and post-yield behavior of high-density polyethylene

    NASA Technical Reports Server (NTRS)

    Semeliss, M. A.; Wong, R.; Tuttle, M. E.

    1990-01-01

    An experimental and analytical evaluation was made of the yield and post-yield behavior of high-density polyethylene, a semi-crystalline thermoplastic. Polyethylene was selected for study because it is very inexpensive and readily available in the form of thin-walled tubes. Thin-walled tubular specimens were subjected to axial loads and internal pressures, such that the specimens were subjected to a known biaxial loading. A constant octahederal shear stress rate was imposed during all tests. The measured yield and post-yield behavior was compared with predictions based on both isotropic and anisotropic models. Of particular interest was whether inelastic behavior was sensitive to the hydrostatic stress level. The major achievements and conclusions reached are discussed.

  7. Maximizing Lipid Yield in Neochloris oleoabundans Algae Extraction by Stressing and Using Multiple Extraction Stages with N-Ethylbutylamine as Switchable Solvent

    PubMed Central

    2017-01-01

    The extraction yield of lipids from nonbroken Neochloris oleoabundans was maximized by using multiple extraction stages and using stressed algae. Experimental parameters that affect the extraction were investigated. The study showed that with wet algae (at least) 18 h extraction time was required for maximum yield at room temperature and a solvent/feed ratio of 1:1 (w/w). For fresh water (FW), nonstressed, nonbroken Neochloris oleoabundans, 13.1 wt % of lipid extraction yield (based on dry algae mass) was achieved, which could be improved to 61.3 wt % for FW stressed algae after four extractions, illustrating that a combination of stressing the algae and applying the solvent N-ethylbutylamine in multiple stages of extraction results in almost 5 times higher yield and is very promising for further development of energy-efficient lipid extraction technology targeting nonbroken wet microalgae. PMID:28781427

  8. A shrunken-2 Transgene Increases Maize Yield by Acting in Maternal Tissues to Increase the Frequency of Seed Development[W

    PubMed Central

    Hannah, L. Curtis; Futch, Brandon; Bing, James; Shaw, Janine R.; Boehlein, Susan; Stewart, Jon D.; Beiriger, Robert; Georgelis, Nikolaos; Greene, Thomas

    2012-01-01

    The maize (Zea mays) shrunken-2 (Sh2) gene encodes the large subunit of the rate-limiting starch biosynthetic enzyme, ADP-glucose pyrophosphorylase. Expression of a transgenic form of the enzyme with enhanced heat stability and reduced phosphate inhibition increased maize yield up to 64%. The extent of the yield increase is dependent on temperatures during the first 4 d post pollination, and yield is increased if average daily high temperatures exceed 33°C. As found in wheat (Triticum aestivum) and rice (Oryza sativa), this transgene increases maize yield by increasing seed number. This result was surprising, since an entire series of historic observations at the whole-plant, enzyme, gene, and physiological levels pointed to Sh2 playing an important role only in the endosperm. Here, we present several lines of evidence that lead to the conclusion that the Sh2 transgene functions in maternal tissue to increase seed number and, in turn, yield. Furthermore, the transgene does not increase ovary number; rather, it increases the probability that a seed will develop. Surprisingly, the number of fully developed seeds is only ∼50% of the number of ovaries in wild-type maize. This suggests that increasing the frequency of seed development is a feasible agricultural target, especially under conditions of elevated temperatures. PMID:22751213

  9. Intermittent illumination increases biophotolytic hydrogen yield by Anabaena cylindrica

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jeffries, T.W.; Leach, K.L.

    Intermittent illumination increased H/sub 2/ and C/sub 2/H/sub 4/ yields per unit of light from growing cells and from nitrogen-starved cells by 1.7- and 1.35-fold, respectively, as compared with continuous illumination.

  10. Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions.

    PubMed

    Driever, Steven M; Simkin, Andrew J; Alotaibi, Saqer; Fisk, Stuart J; Madgwick, Pippa J; Sparks, Caroline A; Jones, Huw D; Lawson, Tracy; Parry, Martin A J; Raines, Christine A

    2017-09-26

    To meet the growing demand for food, substantial improvements in yields are needed. This is particularly the case for wheat, where global yield has stagnated in recent years. Increasing photosynthesis has been identified as a primary target to achieve yield improvements. To increase leaf photosynthesis in wheat, the level of the Calvin-Benson cycle enzyme sedoheptulose-1,7-biphosphatase (SBPase) has been increased through transformation and expression of a Brachypodium distachyon SBPase gene construct. Transgenic lines with increased SBPase protein levels and activity were grown under greenhouse conditions and showed enhanced leaf photosynthesis and increased total biomass and dry seed yield. This showed the potential of improving yield potential by increasing leaf photosynthesis in a crop species such as wheat. The results are discussed with regard to future strategies for further improvement of photosynthesis in wheat.This article is part of the themed issue 'Enhancing photosynthesis in crop plants: targets for improvement'. © 2017 The Authors.

  11. Genotypic Variation in Yield, Yield Components, Root Morphology and Architecture, in Soybean in Relation to Water and Phosphorus Supply

    PubMed Central

    He, Jin; Jin, Yi; Du, Yan-Lei; Wang, Tao; Turner, Neil C.; Yang, Ru-Ping; Siddique, Kadambot H. M.; Li, Feng-Min

    2017-01-01

    Water shortage and low phosphorus (P) availability limit yields in soybean. Roots play important roles in water-limited and P-deficient environment, but the underlying mechanisms are largely unknown. In this study we determined the responses of four soybean [Glycine max (L.) Merr.] genotypes [Huandsedadou (HD), Bailudou (BLD), Jindou 21 (J21), and Zhonghuang 30 (ZH)] to three P levels [applied 0 (P0), 60 (P60), and 120 (P120) mg P kg-1 dry soil to the upper 0.4 m of the soil profile] and two water treatment [well-watered (WW) and water-stressed (WS)] with special reference to root morphology and architecture, we compared yield and its components, root morphology and root architecture to find out which variety and/or what kind of root architecture had high grain yield under P and drought stress. The results showed that water stress and low P, respectively, significantly reduced grain yield by 60 and 40%, daily water use by 66 and 31%, P accumulation by 40 and 80%, and N accumulation by 39 and 65%. The cultivar ZH with the lowest daily water use had the highest grain yield at P60 and P120 under drought. Increased root length was positively associated with N and P accumulation in both the WW and WS treatments, but not with grain yield under water and P deficits. However, in the WS treatment, high adventitious and lateral root densities were associated with high N and P uptake per unit root length which in turn was significantly and positively associated with grain yield. Our results suggest that (1) genetic variation of grain yield, daily water use, P and N accumulation, and root morphology and architecture were observed among the soybean cultivars and ZH had the best yield performance under P and water limited conditions; (2) water has a major influence on nutrient uptake and grain yield, while additional P supply can modestly increase yields under drought in some soybean genotypes; (3) while conserved water use plays an important role in grain yield under drought

  12. Genotypic Variation in Yield, Yield Components, Root Morphology and Architecture, in Soybean in Relation to Water and Phosphorus Supply.

    PubMed

    He, Jin; Jin, Yi; Du, Yan-Lei; Wang, Tao; Turner, Neil C; Yang, Ru-Ping; Siddique, Kadambot H M; Li, Feng-Min

    2017-01-01

    Water shortage and low phosphorus (P) availability limit yields in soybean. Roots play important roles in water-limited and P-deficient environment, but the underlying mechanisms are largely unknown. In this study we determined the responses of four soybean [ Glycine max (L.) Merr.] genotypes [Huandsedadou (HD), Bailudou (BLD), Jindou 21 (J21), and Zhonghuang 30 (ZH)] to three P levels [applied 0 (P0), 60 (P60), and 120 (P120) mg P kg -1 dry soil to the upper 0.4 m of the soil profile] and two water treatment [well-watered (WW) and water-stressed (WS)] with special reference to root morphology and architecture, we compared yield and its components, root morphology and root architecture to find out which variety and/or what kind of root architecture had high grain yield under P and drought stress. The results showed that water stress and low P, respectively, significantly reduced grain yield by 60 and 40%, daily water use by 66 and 31%, P accumulation by 40 and 80%, and N accumulation by 39 and 65%. The cultivar ZH with the lowest daily water use had the highest grain yield at P60 and P120 under drought. Increased root length was positively associated with N and P accumulation in both the WW and WS treatments, but not with grain yield under water and P deficits. However, in the WS treatment, high adventitious and lateral root densities were associated with high N and P uptake per unit root length which in turn was significantly and positively associated with grain yield. Our results suggest that (1) genetic variation of grain yield, daily water use, P and N accumulation, and root morphology and architecture were observed among the soybean cultivars and ZH had the best yield performance under P and water limited conditions; (2) water has a major influence on nutrient uptake and grain yield, while additional P supply can modestly increase yields under drought in some soybean genotypes; (3) while conserved water use plays an important role in grain yield under drought

  13. Effect of salt stress on morpho-physiology, vegetative growth and yield of rice.

    PubMed

    Hakim, M A; Juraimi, Abdul Shukor; Hanafi, M M; Ali, E; Ismail, Mohd Razi; Selamat, Ahmed; Karim, S M Rezaul

    2014-03-01

    Selection of salt tolerant rice varieties has a huge impact on global food supply chain. Five Malaysian rice (Oryza sativa L.) varieties, MR33, MR52, MR211, MR219 and MR232 were tested in pot experiment under different salinity levels for their response in term of vegetative growth, physiological activities, development of yield components and grain yield. Rice varieties, BRRI dhan29 and IR20 were used as a salt-sensitive control and Pokkali was used as a salt-tolerant control. Three different salinity levels viz. 4, 8, and 12 dS m(-1) were used in a randomized complete block design with four replications under glass house conditions. Two Malaysia varieties, MR211 and MR232 performed better in terms of vegetative growth (plant height, leaf area plant(-1), number of tillers plant(-1), dry matter accumulation plant(-1)), photosynthetic rate, transpiration rate, yield components, grain yield and injury symptoms. While, MR33, MR52 and MR219 verities were able to withstand salinity stress over salt-sensitive control, BRRI dhan29 and IR20.

  14. Microwave Energy Increases Fatty Acid Methyl Ester Yield in Human Whole Blood Due to Increased Sphingomyelin Transesterification.

    PubMed

    Metherel, Adam H; Aristizabal Henao, Juan J; Ciobanu, Flaviu; Taha, Ameer Y; Stark, Ken D

    2015-09-01

    Dried blood spots (DBS) by fingertip prick collection for fatty acid profiling are becoming increasingly popular due to ease of collection, minimal invasiveness and its amenability to high-throughput analyses. Herein, we assess a microwave-assisted direct transesterification method for the production of fatty acid methyl esters (FAME) from DBS. Technical replicates of human whole blood were collected and 25-μL aliquots were applied to chromatography strips prior to analysis by a standard 3-h transesterification method or microwave-assisted direct transesterification method under various power (variable vs constant), time (1-5 min) and reagent (1-10% H2SO4 in methanol) conditions. In addition, a standard method was compared to a 5-min, 30-W power microwave in 1% H2SO4 method for FAME yield from whole blood sphingomyelin, and sphingomyelin standards alone and spiked in whole blood. Microwave-assisted direct transesterification yielded no significant differences in both quantitative (nmol/100 µL) and qualitative (mol%) fatty acid assessments after as little as 1.5- and 1-min reaction times, respectively, using the variable power method and 5% H2SO4 in methanol. However, 30-W power for 5 min increased total FAME yield of the technical replicates by 14%. This increase appears largely due to higher sphingomyelin-derived FAME yield of up to 109 and 399% compared to the standard method when determined from whole blood or pure standards, respectively. In conclusion, microwave-assisted direct transesterification of DBS achieved in as little as 1-min, and 5-min reaction times increase total fatty acids primarily by significantly improving sphingomyelin-derived fatty acid yield.

  15. Stress increases descending inhibition in mouse and human colon.

    PubMed

    Reed, D E; Zhang, Y; Beyak, M J; Lourenssen, S; Blennerhassett, M G; Paterson, W G; Vanner, S J

    2016-04-01

    A relationship between stress and the symptoms of irritable bowel syndrome (IBS) has been well established but the cellular mechanisms are poorly understood. Therefore, we investigated effects of stress and stress hormones on colonic descending inhibition and transit in mouse models and human tissues. Stress was applied using water avoidance stress (WAS) in the animal model or mimicked using stress hormones, adrenaline (5 nM), and corticosterone (1 μM). Intracellular recordings were obtained from colonic circular smooth muscle cells in isolated smooth muscle/myenteric plexus preparations and the inhibitory junction potential (IJP) was elicited by nerve stimulation or balloon distension oral to the site of recording. Water avoidance stress increased the number of fecal pellets compared to control (p < 0.05). WAS also caused a significant increase in IJP amplitude following balloon distension. Stress hormones also increased the IJP amplitude following nerve stimulation and balloon distension (p < 0.05) in control mice but had no effect in colons from stressed mice. No differences were observed with application of ATP between stress and control tissues, suggesting the actions of stress hormones were presynaptic. Stress hormones had a large effect in the nerve stimulated IJP in human colon (increased >50%). Immunohistochemical studies identified alpha and beta adrenergic receptor immunoreactivity on myenteric neurons in human colon. These studies suggest that WAS and stress hormones can signal via myenteric neurons to increase inhibitory neuromuscular transmission. This could lead to greater descending relaxation, decreased transit time, and subsequent diarrhea. © 2016 John Wiley & Sons Ltd.

  16. Effect of salinity stress on phenotypic plasticity, yield stability, and signature of stable isotopes of carbon and nitrogen in safflower.

    PubMed

    Hussain, Muhammad Iftikhar; Al-Dakheel, Abdullah J

    2018-06-05

    Salinity is one of the major factors contributing in land degradation, disturbance of soil biology, a structure that leads to unproductive land with low crop yield potential especially in arid and semiarid regions of the world. Appropriate crops with sufficient stress tolerance capacity and non-conventional water resources should have to be managed in a sustainable way to bring these marginal lands under cultivation for future food security. The goal of the present study was to evaluate salinity tolerant potential (0, 7, and 14 dS m -1 ) of six safflower genotypes that can be adapted to the hyper arid climate of UAE and its marginal soil. Several agro-morphological and physiological traits such as plant dry biomass (PDM), number of branches (BN), number of capitula (CN), seed yield (SY), stable isotope composition of nitrogen (δ 15 N) and carbon (δ 13 C), intercellular CO 2 concentration from inside to ambient air (Ci/Ca), intrinsic water use efficiency (iWUE), carbon (C%) and nitrogen (N %), and harvest index (HI) were evaluated as indicative of the functional performance of safflower genotypes under salt stress. Results indicated that salinity significantly affected the seed yield at all levels and varied significantly among genotypes. The BN, PDM, CN, and δ 13 C attributes showed clear differentiation between tolerant and susceptible genotypes. The δ 13 C results indicate that the tolerant genotypes suffer less from stress, may be due to better rooting. Tolerant genotypes showed lower iWUE values but possess higher yield. Safflower genotypes (PI248836 and PI167390) proved to be salt tolerant, stable, and higher seed and biomass yielder. There was no G × E interaction but the genotypes that produce higher yield under control were still best even under salt stress conditions. Although salinity reduced crop yield, some tolerant genotypes demonstrate adaptation and good yield potential under saline marginal environment.

  17. Increased gluconeogenesis in hyper-G stressed rats

    NASA Technical Reports Server (NTRS)

    Daligcon, B. C.; Oyama, J.

    1982-01-01

    The role of gluconeogenesis in the altered carbohydrate metabolism in rats exposed to hyper-G stress is investigated. The blood levels of the substrates and hormones involved in gluconeogenesis were determined in rats exposed to 3.1 G for various time periods (0.25 to 24 hr). It is found that hyper-G stressed rats showed an immediate increase in plasma glucose at the onset of centrifugation which persisted throughout all the exposure periods. A substantial part of the initial rise in blood glucose is attributed to an increased rate of gluconeogenesis. An increase in liver glycogen deposition was observed in centrifuged rats as early as 0.50 hr exposure time, with progressively larger amounts accumulated as the exposure time was extended to 24 hr. It is concluded that the increase in gluconeogenic activity of hyper-G stressed rats is due to an increase in the mobilization of gluconeogenic substrates from perpheral tissues to the liver as a result of increases in circulating catecholamines and glucagon.

  18. Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for improving growth and yield of wheat (Triticum aestivum L.) under salt-stressed conditions.

    PubMed

    Zahir, Zahir Ahmad; Ghani, Usman; Naveed, Muhammad; Nadeem, Sajid Mahmood; Asghar, Hafiz Naeem

    2009-05-01

    Ethylene synthesis is accelerated in response to various environmental stresses like salinity. Ten rhizobacterial strains isolated from wheat rhizosphere taken from different salt affected areas were screened for growth promotion of wheat under axenic conditions at 1, 5, 10 and 15 dS m(-1). Three strains, i.e., Pseudomonas putida (N21), Pseudomonas aeruginosa (N39) and Serratia proteamaculans (M35) showing promising performance under axenic conditions were selected for a pot trial at 1.63 (original), 5, 10 and 15 dS m(-1). Results showed that inoculation was effective even in the presence of higher salinity levels. P. putida was the most efficient strain compared to the other strains and significantly increased the plant height, root length, grain yield, 100-grain weight and straw yield up to 52, 60, 76, 19 and 67%, respectively, over uninoculated control at 15 dS m(-1). Similarly, chlorophyll content and K(+)/Na(+) of leaves also increased by P. putida over control. It is highly likely that under salinity stress, 1-aminocyclopropane-1-carboxylic acid-deaminase activity of these microbial strains might have caused reduction in the synthesis of stress (salt)-induced inhibitory levels of ethylene. The results suggested that these strains could be employed for salinity tolerance in wheat; however, P. putida may have better prospects in stress alleviation/reduction.

  19. Yield Hardening of Electrorheological Fluids in Channel Flow

    NASA Astrophysics Data System (ADS)

    Helal, Ahmed; Qian, Bian; McKinley, Gareth H.; Hosoi, A. E.

    2016-06-01

    Electrorheological fluids offer potential for developing rapidly actuated hydraulic devices where shear forces or pressure-driven flow are present. In this study, the Bingham yield stress of electrorheological fluids with different particle volume fractions is investigated experimentally in wall-driven and pressure-driven flow modes using measurements in a parallel-plate rheometer and a microfluidic channel, respectively. A modified Krieger-Dougherty model can be used to describe the effects of the particle volume fraction on the yield stress and is in good agreement with the viscometric data. However, significant yield hardening in pressure-driven channel flow is observed and attributed to an increase and eventual saturation of the particle volume fraction in the channel. A phenomenological physical model linking the densification and consequent microstructure to the ratio of the particle aggregation time scale compared to the convective time scale is presented and used to predict the enhancement in yield stress in channel flow, enabling us to reconcile discrepancies in the literature between wall-driven and pressure-driven flows.

  20. Yield surface evolution for columnar ice

    NASA Astrophysics Data System (ADS)

    Zhou, Zhiwei; Ma, Wei; Zhang, Shujuan; Mu, Yanhu; Zhao, Shunpin; Li, Guoyu

    A series of triaxial compression tests, which has capable of measuring the volumetric strain of the sample, were conducted on columnar ice. A new testing approach of probing the experimental yield surface was performed from a single sample in order to investigate yield and hardening behaviors of the columnar ice under complex stress states. Based on the characteristic of the volumetric strain, a new method of defined the multiaxial yield strengths of the columnar ice is proposed. The experimental yield surface remains elliptical shape in the stress space of effective stress versus mean stress. The effect of temperature, loading rate and loading path in the initial yield surface and deformation properties of the columnar ice were also studied. Subsequent yield surfaces of the columnar ice have been explored by using uniaxial and hydrostatic paths. The evolution of the subsequent yield surface exhibits significant path-dependent characteristics. The multiaxial hardening law of the columnar ice was established experimentally. A phenomenological yield criterion was presented for multiaxial yield and hardening behaviors of the columnar ice. The comparisons between the theoretical and measured results indicate that this current model is capable of giving a reasonable prediction for the multiaxial yield and post-yield properties of the columnar ice subjected to different temperature, loading rate and path conditions.

  1. Increasing temperature cuts back crop yields in Hungary over the last 90 years.

    PubMed

    Pinke, Zsolt; Lövei, Gábor L

    2017-12-01

    The transformation of climatic regime has an undeniable impact on plant production, but we rarely have long enough date series to examine the unfolding of such effects. The clarification of the relationship between crop plants and climate has a near-immediate importance due to the impending human-made global change. This study investigated the relationship between temperature, precipitation, drought intensity and the yields of four major cereals in Hungary between 1921 and 2010. The analysis of 30-year segments indicated a monotonously increasing negative impact of temperature on crop yields. A 1°C temperature increase reduced the yield of the four main cereals by 9.6%-14.8% in 1981-2010, which revealed the vulnerability of Eastern European crop farming to recent climate change. Climate accounted for 17%-39% of yield variability over the past 90 years, but this figure reached 33%-67% between 1981 and 2010. Our analysis supports the claim that the mid-20th century green revolution improved yields "at the mercy of the weather": during this period, the impact of increasing fertilization and mechanisation coincided with climatic conditions that were more favourable than today. Crop yields in Eastern Europe have been stagnating or decreasing since the mid-1980s. Although usually attributed to the large socio-economic changes sweeping the region, our analysis indicates that a warming climate is at least partially responsible for this trend. Such a robust impact of increasing temperatures on crop yields also constitutes an obvious warning for this core grain-growing region of the world. © 2017 John Wiley & Sons Ltd.

  2. Use of the Richtmyer-Meshkov Instability to Infer Yield Stress at High-Energy Densities

    NASA Astrophysics Data System (ADS)

    Dimonte, Guy; Terrones, G.; Cherne, F. J.; Germann, T. C.; Dupont, V.; Kadau, K.; Buttler, W. T.; Oro, D. M.; Morris, C.; Preston, D. L.

    2011-12-01

    We use the Richtmyer-Meshkov instability (RMI) at a metal-gas interface to infer the metal’s yield stress (Y) under shock loading and release. We first model how Y stabilizes the RMI using hydrodynamics simulations with a perfectly plastic constitutive relation for copper (Cu). The model is then tested with molecular dynamics (MD) of crystalline Cu by comparing the inferred Y from RMI simulations with direct stress-strain calculations, both with MD at the same conditions. Finally, new RMI experiments with solid Cu validate our simulation-based model and infer Y˜0.47GPa for a 36 GPa shock.

  3. Ectopic Expression of CDF3 Genes in Tomato Enhances Biomass Production and Yield under Salinity Stress Conditions

    PubMed Central

    Renau-Morata, Begoña; Molina, Rosa V.; Carrillo, Laura; Cebolla-Cornejo, Jaime; Sánchez-Perales, Manuel; Pollmann, Stephan; Domínguez-Figueroa, José; Corrales, Alba R.; Flexas, Jaume; Vicente-Carbajosa, Jesús; Medina, Joaquín; Nebauer, Sergio G.

    2017-01-01

    Cycling Dof Factor (CDF) transcription factors (TFs) are involved in multiple processes related to plant growth and development. A member of this family, CDF3, has recently been linked in Arabidopsis to the regulation of primary metabolism and abiotic stress responses, but its role in crop production under stress is still unknown. In this study, we characterized tomato plants overexpressing the CDF3 genes from Arabidopsis and tomato and analyzed their effects on growth and yield under salinity, additionally gaining deeper insights into the molecular function of these TFs. Our results provide evidence for higher biomass production and yield in the 35S::AtCDF3 and 35S::SlCDF3 plants, likely due to a higher photosynthetic capacity resulting in increased sucrose availability. Transcriptome analysis revealed that CDF3 genes regulate a set of genes involved in redox homeostasis, photosynthesis performance and primary metabolism that lead to enhanced biomass production. Consistently, metabolomic profiling revealed that CDF3 evokes changes in the primary metabolism triggering enhanced nitrogen assimilation, and disclosed that the amount of some protective metabolites including sucrose, GABA and asparagine were higher in vegetative tissues of CDF3 overexpressing plants. Altogether these changes improved performance of 35S::AtCDF3 and 35S::SlCDF3 plants under salinity conditions. Moreover, the overexpression of CDF3 genes modified organic acid and sugar content in fruits, improving variables related to flavor perception and fruit quality. Overall, our results associate the CDF3 TF with a role in the control of growth and C/N metabolism, and highlight that overexpression of CDF3 genes can substantially improve plant yield. PMID:28515731

  4. Temperature Increase Reduces Global Yields of Major Crops in Four Independent Estimates

    NASA Technical Reports Server (NTRS)

    Zhao, Chuang; Liu, Bing; Piao, Shilong; Wang, Xuhui; Lobell, David B.; Huang, Yao; Huang, Mengtian; Yao, Yitong; Bassu, Simona; Ciais, Philippe; hide

    2017-01-01

    Wheat, rice, maize, and soybean provide two-thirds of human caloric intake. Assessing the impact of global temperature increase on production of these crops is therefore critical to maintaining global food supply, but different studies have yielded different results. Here, we investigated the impacts of temperature on yields of the four crops by compiling extensive published results from four analytical methods: global grid-based and local point-based models, statistical regressions, and field-warming experiments. Results from the different methods consistently showed negative temperature impacts on crop yield at the global scale, generally underpinned by similar impacts at country and site scales. Without CO2 fertilization, effective adaptation, and genetic improvement, each degree-Celsius increase in global mean temperature would, on average, reduce global yields of wheat by 6.0%, rice by 3.2%, maize by 7.4%, and soybean by 3.1%. Results are highly heterogeneous across crops and geographical areas, with some positive impact estimates. Multi-method analyses improved the confidence in assessments of future climate impacts on global major crops and suggest crop- and region-specific adaptation strategies to ensure food security for an increasing world population.

  5. Temperature increase reduces global yields of major crops in four independent estimates

    PubMed Central

    Zhao, Chuang; Piao, Shilong; Wang, Xuhui; Lobell, David B.; Huang, Yao; Huang, Mengtian; Yao, Yitong; Bassu, Simona; Ciais, Philippe; Durand, Jean-Louis; Elliott, Joshua; Ewert, Frank; Janssens, Ivan A.; Li, Tao; Lin, Erda; Liu, Qiang; Martre, Pierre; Peng, Shushi; Wallach, Daniel; Wang, Tao; Wu, Donghai; Liu, Zhuo; Zhu, Yan; Zhu, Zaichun; Asseng, Senthold

    2017-01-01

    Wheat, rice, maize, and soybean provide two-thirds of human caloric intake. Assessing the impact of global temperature increase on production of these crops is therefore critical to maintaining global food supply, but different studies have yielded different results. Here, we investigated the impacts of temperature on yields of the four crops by compiling extensive published results from four analytical methods: global grid-based and local point-based models, statistical regressions, and field-warming experiments. Results from the different methods consistently showed negative temperature impacts on crop yield at the global scale, generally underpinned by similar impacts at country and site scales. Without CO2 fertilization, effective adaptation, and genetic improvement, each degree-Celsius increase in global mean temperature would, on average, reduce global yields of wheat by 6.0%, rice by 3.2%, maize by 7.4%, and soybean by 3.1%. Results are highly heterogeneous across crops and geographical areas, with some positive impact estimates. Multimethod analyses improved the confidence in assessments of future climate impacts on global major crops and suggest crop- and region-specific adaptation strategies to ensure food security for an increasing world population. PMID:28811375

  6. Temperature increase reduces global yields of major crops in four independent estimates.

    PubMed

    Zhao, Chuang; Liu, Bing; Piao, Shilong; Wang, Xuhui; Lobell, David B; Huang, Yao; Huang, Mengtian; Yao, Yitong; Bassu, Simona; Ciais, Philippe; Durand, Jean-Louis; Elliott, Joshua; Ewert, Frank; Janssens, Ivan A; Li, Tao; Lin, Erda; Liu, Qiang; Martre, Pierre; Müller, Christoph; Peng, Shushi; Peñuelas, Josep; Ruane, Alex C; Wallach, Daniel; Wang, Tao; Wu, Donghai; Liu, Zhuo; Zhu, Yan; Zhu, Zaichun; Asseng, Senthold

    2017-08-29

    Wheat, rice, maize, and soybean provide two-thirds of human caloric intake. Assessing the impact of global temperature increase on production of these crops is therefore critical to maintaining global food supply, but different studies have yielded different results. Here, we investigated the impacts of temperature on yields of the four crops by compiling extensive published results from four analytical methods: global grid-based and local point-based models, statistical regressions, and field-warming experiments. Results from the different methods consistently showed negative temperature impacts on crop yield at the global scale, generally underpinned by similar impacts at country and site scales. Without CO 2 fertilization, effective adaptation, and genetic improvement, each degree-Celsius increase in global mean temperature would, on average, reduce global yields of wheat by 6.0%, rice by 3.2%, maize by 7.4%, and soybean by 3.1%. Results are highly heterogeneous across crops and geographical areas, with some positive impact estimates. Multimethod analyses improved the confidence in assessments of future climate impacts on global major crops and suggest crop- and region-specific adaptation strategies to ensure food security for an increasing world population.

  7. Using FACE systems to screen wheat cultivars for yield increases at elevated CO2

    USDA-ARS?s Scientific Manuscript database

    Because of continuing increases in atmospheric CO2, identifying cultivars of crops with larger yield increases at elevated CO2 may provide an avenue to increase crop yield potential in future climates. Free-air CO2 enrichment (FACE) systems have most often been used with multiple replications of ea...

  8. Socioeconomic status and stress-induced increases in interleukin-6.

    PubMed

    Brydon, L; Edwards, S; Mohamed-Ali, V; Steptoe, A

    2004-05-01

    Coronary artery disease (CAD) is more prevalent in people from a low socioeconomic background, and low socioeconomic status (SES) is associated with an increased exposure to psychological stress. The pro-inflammatory cytokine interleukin-6 (IL-6) plays a central role in CAD development. IL-6 is responsive to psychological stress and could potentially mediate the effect of psychosocial factors on CAD risk. Accordingly, we predicted that people of low SES would have greater and/or more sustained IL-6 responses to acute psychological stress. Based on previous findings, we also predicted that these people would have delayed post-stress cardiovascular recovery. Thirty-eight male civil servants were tested, with participants divided into high and low SES groups according to employment grade. There were no differences between the groups at baseline. However there were significant differences in IL-6 and heart rate responses to stress. Stress induced increases in plasma IL-6 in all participants. However, in the low SES group, IL-6 continued to increase between 75 min and 2h post-stress, whereas IL-6 levels stabilised at 75 min in the high SES group. Heart rate increased to the same extent following stress in both groups, however by 2h post-stress, it had returned to baseline in 75% of the high SES group compared with only 38.1% of the low SES group. These results suggest that low SES people are less able to adapt to stress than their high SES counterparts. Prolonged stress-induced increases in IL-6 in low SES groups represents a novel mechanism potentially linking socioeconomic position and heart disease.

  9. Physiological Traits Associated with Wheat Yield Potential and Performance under Water-Stress in a Mediterranean Environment

    PubMed Central

    del Pozo, Alejandro; Yáñez, Alejandra; Matus, Iván A.; Tapia, Gerardo; Castillo, Dalma; Sanchez-Jardón, Laura; Araus, José L.

    2016-01-01

    Different physiological traits have been proposed as key traits associated with yield potential as well as performance under water stress. The aim of this paper is to examine the genotypic variability of leaf chlorophyll, stem water-soluble carbohydrate content and carbon isotope discrimination (Δ13C), and their relationship with grain yield (GY) and other agronomical traits, under contrasting water conditions in a Mediterranean environment. The study was performed on a large collection of 384 wheat genotypes grown under water stress (WS, rainfed), mild water stress (MWS, deficit irrigation), and full irrigation (FI). The average GY of two growing seasons was 2.4, 4.8, and 8.9 Mg ha−1 under WS, MWS, and FI, respectively. Chlorophyll content at anthesis was positively correlated with GY (except under FI in 2011) and the agronomical components kernels per spike (KS) and thousand kernel weight (TKW). The WSC content at anthesis (WSCCa) was negatively correlated with spikes per square meter (SM2), but positively correlated with KS and TKW under WS and FI conditions. As a consequence, the relationships between WSCCa with GY were low or not significant. Therefore, selecting for high stem WSC would not necessary lead to genotypes of GY potential. The relationship between Δ13C and GY was positive under FI and MWS but negative under severe WS (in 2011), indicating higher water use under yield potential and MWS conditions. PMID:27458470

  10. Physiological Traits Associated with Wheat Yield Potential and Performance under Water-Stress in a Mediterranean Environment.

    PubMed

    Del Pozo, Alejandro; Yáñez, Alejandra; Matus, Iván A; Tapia, Gerardo; Castillo, Dalma; Sanchez-Jardón, Laura; Araus, José L

    2016-01-01

    Different physiological traits have been proposed as key traits associated with yield potential as well as performance under water stress. The aim of this paper is to examine the genotypic variability of leaf chlorophyll, stem water-soluble carbohydrate content and carbon isotope discrimination (Δ(13)C), and their relationship with grain yield (GY) and other agronomical traits, under contrasting water conditions in a Mediterranean environment. The study was performed on a large collection of 384 wheat genotypes grown under water stress (WS, rainfed), mild water stress (MWS, deficit irrigation), and full irrigation (FI). The average GY of two growing seasons was 2.4, 4.8, and 8.9 Mg ha(-1) under WS, MWS, and FI, respectively. Chlorophyll content at anthesis was positively correlated with GY (except under FI in 2011) and the agronomical components kernels per spike (KS) and thousand kernel weight (TKW). The WSC content at anthesis (WSCCa) was negatively correlated with spikes per square meter (SM2), but positively correlated with KS and TKW under WS and FI conditions. As a consequence, the relationships between WSCCa with GY were low or not significant. Therefore, selecting for high stem WSC would not necessary lead to genotypes of GY potential. The relationship between Δ(13)C and GY was positive under FI and MWS but negative under severe WS (in 2011), indicating higher water use under yield potential and MWS conditions.

  11. Characterization of mixing and yield stress of pretreated wheat straw slurries used for the production of biofuels through tomography technique.

    PubMed

    Naghavi-Anaraki, Yasaman; Turcotte, Ginette; Ein-Mozaffari, Farhad

    2018-05-29

    Wheat straw is a low-cost feedstock for the production of biofuel. Pretreatment process is an important stage in producing biofuels since it makes the fibers more accessible to enzymatic hydrolysis which is the final step of producing biofuels. Pretreated wheat straw (PWS) slurries are non-Newtonian fluids with yield stress. Mixing of fluids exhibiting yield stress such as the pretreated wheat straw slurry results in the generation of cavern, which is a fully-mixed zone, around the impeller and the stationary regions elsewhere, which causes difficulties in the production of biofuels. In this study, the non-invasive electrical resistance tomography technique was utilized to determine the cavern dimensions as a function of the impeller type and impeller speed. The cavern sizes were then used to measure the yield stress of PWS slurries as a function of fiber size (≤ 2 and ≤ 6 mm) and fiber concentration (6, 8, and 10 wt%).

  12. On the determination of a generalized Darcy equation for yield stress fluid in porous media using a LB TRT scheme

    NASA Astrophysics Data System (ADS)

    Talon, Laurent; Chevalier, Thibaud

    2014-11-01

    Non-Newtonian fluids have practical applications in very different domains. Indeed, polymer mixture, paints, slurries, colloidal suspensions, emulsions, foams or heavy oil present complex rheologies. Among the large number of different non-Newtonian fluids an important class of behavior is represented by the yield-stress fluids, viz. fluids that require a minimum of stress to flow. Yield stress fluids are usually modelled as a Bingham fluid or by the Herschel-Bulkley equation. However, simulating flow of a Bingham fluid in porous media still remains a challenging task as the yield stress may significantly alter the numerical stability and precision. In the present work, we use a Lattice-Boltzmann TRT scheme to determine this type of flow in a synthetic porous medium or fracture. Different pressure drops ΔP have been applied in order to derive a generalization of the Darcy's equation. Three different scaling regimes can be distinguished when plotting the dimensionless flow rate q as function of the distance to the critical pressure ΔP - ΔPc . In this presentation, we will investigate the importance of the heterogeneities on those flowing regimes. ANR-12-MONU-0011.

  13. Dynamic Yielding and Spall Behavior of Commercially Pure Grade 4 Titanium

    NASA Astrophysics Data System (ADS)

    Thadhani, Naresh; Whelchel, R. L.; Sanders, Tom; Mehkote, D. S.; Iyer, K. A.; Georgia Instiutute of Technology Collaboration; Johns Hopkins University, Applied Physics Labortaory Collaboration

    2015-06-01

    The dynamic yielding and fracture (spalling) of commercially pure (grade 4) titanium are investigated using symmetric plate impact experiments over a peak stress range of 5.6 GPa to 12.5 GPa, using the 80-mm single-stage gas-gun. VISAR rear free surface velocity profiles display both a Hugoniot elastic limit (HEL) and a velocity pullback, which are indicative of dynamic compressive yielding and tensile fracture (spalling), respectively. The HEL values appear to show a slight decrease with peak stress from 2.2 GPa to 2.0 GPa along with a corresponding increase in twinning observed in recovered impacted samples. The spall strength on the other hand increases with peak stress from a value of 3.3 GPa to 3.8 GPa and shows a good power law fit with the decompression strain rate. The differing responses in dynamic yield and fracture behavior suggest that void nucleation may be the dominant mechanism affecting the spall strength of grade 4 titanium.

  14. Assessing disease stress and modeling yield losses in alfalfa

    NASA Astrophysics Data System (ADS)

    Guan, Jie

    weight, percentage reflectance (810 nm), and green leaf area index (GLAI). Percentage reflectance (810 nm) assessments had a stronger relationship with dry weight and green leaf area index than percentage defoliation assessments. Our research conclusively demonstrates that percentage reflectance measurements can be used to nondestructively assess green leaf area index which is a direct measure of plant health and an indirect measure of productivity. This research conclusively demonstrates that remote sensing is superior to visual assessment method to assess alfalfa stress and to model yield and GLAI in the alfalfa foliar disease pathosystem.

  15. Tribology behavior on scratch tests: Effects of yield strength

    DOE PAGES

    Feng, Biao

    2017-03-07

    In this paper, a three-dimensional (3D) scratch model is proposed to investigate the effects of yield strength of both coatings and substrates. With the help of combined Coulomb and plastic friction, the obtained results comprehensively interpret the experimental phenomena in most metals that with the growth of hardness after heat treatment the scratch friction coefficient (SFC) increases. This interpretation could not be done before. Scratch tests on the surface with or without the coating are discussed. Without the coating the SFC increases due to the decrease of the area with plastic slippage and/or the increase of friction stress during themore » increase of the yield strength in the material. With a softer substrate the friction stress decreases but the SFC increases, which is caused by the growth of the entire contact area and surface deformation. Conversely, with a stronger substrate the SFC decreases due to an intensified plastic slippage In conclusion, the obtained results pave a new way to understanding the effects of yield strength on scratch tests, interpret experimental phenomena, and should be helpful for an optimum design in experiments.« less

  16. Tribology behavior on scratch tests: Effects of yield strength

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Feng, Biao

    In this paper, a three-dimensional (3D) scratch model is proposed to investigate the effects of yield strength of both coatings and substrates. With the help of combined Coulomb and plastic friction, the obtained results comprehensively interpret the experimental phenomena in most metals that with the growth of hardness after heat treatment the scratch friction coefficient (SFC) increases. This interpretation could not be done before. Scratch tests on the surface with or without the coating are discussed. Without the coating the SFC increases due to the decrease of the area with plastic slippage and/or the increase of friction stress during themore » increase of the yield strength in the material. With a softer substrate the friction stress decreases but the SFC increases, which is caused by the growth of the entire contact area and surface deformation. Conversely, with a stronger substrate the SFC decreases due to an intensified plastic slippage In conclusion, the obtained results pave a new way to understanding the effects of yield strength on scratch tests, interpret experimental phenomena, and should be helpful for an optimum design in experiments.« less

  17. High-resolution mapping of yield curve shape and evolution for high porosity sandstones

    NASA Astrophysics Data System (ADS)

    Bedford, J. D.; Faulkner, D.; Wheeler, J.; Leclere, H.

    2017-12-01

    The onset of permanent inelastic deformation for porous rock is typically defined by a yield curve plotted in P-Q space, where P is the effective mean stress and Q is the differential stress. Sandstones usually have broadly elliptical shaped yield curves, with the low pressure side of the ellipse associated with localized brittle faulting (dilation) and the high pressure side with distributed ductile deformation (compaction). However recent works have shown that these curves might not be perfectly elliptical and that significant evolution in shape occurs with continued deformation. We therefore use a novel stress-probing methodology to map in high-resolution the yield curve shape for Boise and Idaho Gray sandstones (36-38% porosity) and also investigate curve evolution with increasing deformation. The data reveal yield curves with a much flatter geometry than previously recorded for porous sandstone and that the compactive side of the curve is partly comprised of a near vertical limb. The yield curve evolution is found to be strongly dependent on the nature of inelastic strain. Samples that were compacted under a deviatoric load, with a component of inelastic shear strain, were found to have yield curves with peaks that are approximately 50% higher than similar porosity samples that were hydrostatically compacted (i.e. purely volumetric strain). The difference in yield curve evolution along the different loading paths is attributed to mechanical anisotropy that develops during deviatoric loading by the closure of preferentially orientated fractures. Increased shear strain also leads to the formation of a plateau at the peak of the yield curve as samples deform along the deviatoric loading path. These results have important implications for understanding how the strength of porous rock evolves along different stress paths, including during fluid extraction from hydrocarbon reservoirs where the stress state is rarely isotropic.

  18. Increased Night Temperature Negatively Affects Grain Yield, Biomass and Grain Number in Chilean Quinoa

    PubMed Central

    Lesjak, Jurka; Calderini, Daniel F.

    2017-01-01

    Quinoa high nutritive value increases interest worldwide, especially as a crop that could potentially feature in different cropping systems, however, climate change, particularly rising temperatures, challenges this and other crop species. Currently, only limited knowledge exists regarding the grain yield and other key traits response to higher temperatures of this crop, especially to increased night temperatures. In this context, the main objective of this study was to evaluate the effect of increased night temperature on quinoa yield, grain number, individual grain weight and processes involved in crop growth under the environmental conditions (control treatment) and night thermal increase at two phases: flowering (T1) and grain filling (T2) in southern Chile. A commercial genotype, Regalona, and a quinoa accession (Cod. BO5, N°191, grain bank from Semillas Baer, hereby referred to as Accession) were used, due to their adaptability to Southern Chilean conditions and contrasting grain yield potential, grain weight and size of plants. Temperature was increased ≈4°C above the ambient from 8 pm until 9 am the next morning. Control treatments reached a high grain yield (600 and 397 g m-2, i.e., Regalona and Accession). Temperature increase reduced grain yield by 31% under T1 treatment and 12% when under T2 in Regalona and 23 and 26% in Accession, respectively. Aboveground biomass was negatively affected by the thermal treatments and a positive linear association was found between grain yield and aboveground biomass across treatments. By contrast, the harvest index was unaffected either by genotype, or by thermal treatments. Grain number was significantly affected between treatments and this key trait was linearly associated with grain yield. On the other hand, grain weight showed a narrow range of variation across treatments. Additionally, leaf area index was not affected, but significant differences were found in SPAD values at the end of T1 treatment, compared

  19. Rhizosphere bacteria containing 1-aminocyclopropane-1-carboxylate deaminase increase yield of plants grown in drying soil via both local and systemic hormone signalling.

    PubMed

    Belimov, Andrey A; Dodd, Ian C; Hontzeas, Nikos; Theobald, Julian C; Safronova, Vera I; Davies, William J

    2009-01-01

    Decreased soil water availability can stimulate production of the plant hormone ethylene and inhibit plant growth. Strategies aimed at decreasing stress ethylene evolution might attenuate its negative effects. An environmentally benign (nonchemical) method of modifying crop ethylene relations - soil inoculation with a natural root-associated bacterium Variovorax paradoxus 5C-2 (containing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase that degrades the ethylene precursor ACC), was assessed with pea (Pisum sativum) plants grown in drying soil. Inoculation with V. paradoxus 5C-2, but not with a transposome mutant with massively decreased ACC deaminase activity, improved growth, yield and water-use efficiency of droughted peas. Systemic effects of V. paradoxus 5C-2 included an amplified soil drying-induced increase of xylem abscisic acid (ABA) concentration, but an attenuated soil drying-induced increase of xylem ACC concentration. A local bacterial effect was increased nodulation by symbiotic nitrogen-fixing bacteria, which prevented a drought-induced decrease in nodulation and seed nitrogen content. Successfully deploying a single bacterial gene in the rhizosphere increased yield and nutritive value of plants grown in drying soil, via both local and systemic hormone signalling. Such bacteria may provide an easily realized, economic means of sustaining crop yields and using irrigation water more efficiently in dryland agriculture.

  20. Satellite-based mapping of field-scale stress indicators for crop yield forecasting: an application over Mead, NE

    NASA Astrophysics Data System (ADS)

    Yang, Y.; Anderson, M. C.; Gao, F.; Wardlow, B.; Hain, C.; Otkin, J.; Sun, L.; Dulaney, W.

    2017-12-01

    In agricultural regions, water is one of the most widely limiting factors of crop performance and production. Evapotranspiration (ET) describes crop water use through transpiration and water lost through direct soil evaporation, which makes it a good indicator of soil moisture availability and vegetation health and thus has been an integral part of many yield estimation efforts. The Evaporative Stress Index (ESI) describes temporal anomalies in a normalized evapotranspiration metric (fRET) as derived from satellite remote sensing and has demonstrated capacity to explain regional yield variability in water limited crop growing regions. However, its performance in some regions where the vegetation cycle is intensively managed appears to be degraded. In this study we generated maps of ET, fRET, and ESI at high spatiotemporal resolution (30-m pixels, daily timesteps) using a multi-sensor data fusion method, integrating information from satellite platforms with good temporal coverage and other platforms that provide field-scale spatial detail. The study was conducted over the period 2010-2014, covering a region around Mead, Nebraska that includes both rainfed and irrigated crops. Correlations between ESI and measurements of corn yield are investigated at both the field and county level to assess the value of ESI as a yield forecasting tool. To examine the role of phenology in ESI-yield correlations, annual input fRET timeseries were aligned by both calendar day and by biophysically relevant dates (e.g. days since planting or emergence). Results demonstrate that mapping of fRET and ESI at 30-m has the advantage of being able to resolve different crop types with varying phenology. The study also suggests that incorporating phenological information significantly improves yield-correlations by accounting for effects of phenology such as variable planting date and emergence date. The yield-ESI relationship in this study well captures the inter-annual variability of yields

  1. Effect of simultaneous drought stress and root-knot nematode infection on cotton yield and fiber quality

    USDA-ARS?s Scientific Manuscript database

    Both drought stress and root-knot nematode (Meloidogyne incognita) infection can reduce cotton yield, and drought can affect fiber quality, but it not known what effect the nematodes have on fiber quality. To determine whether nematode parasitism affects fiber quality and whether the combined effec...

  2. Bats and birds increase crop yield in tropical agroforestry landscapes.

    PubMed

    Maas, Bea; Clough, Yann; Tscharntke, Teja

    2013-12-01

    Human welfare is significantly linked to ecosystem services such as the suppression of pest insects by birds and bats. However, effects of biocontrol services on tropical cash crop yield are still largely unknown. For the first time, we manipulated the access of birds and bats in an exclosure experiment (day, night and full exclosures compared to open controls in Indonesian cacao agroforestry) and quantified the arthropod communities, the fruit development and the final yield over a long time period (15 months). We found that bat and bird exclusion increased insect herbivore abundance, despite the concurrent release of mesopredators such as ants and spiders, and negatively affected fruit development, with final crop yield decreasing by 31% across local (shade cover) and landscape (distance to primary forest) gradients. Our results highlight the tremendous economic impact of common insectivorous birds and bats, which need to become an essential part of sustainable landscape management. © 2013 John Wiley & Sons Ltd/CNRS.

  3. Lowering rumen-degradable protein maintained energy-corrected milk yield and improved nitrogen-use efficiency in multiparous lactating dairy cows exposed to heat stress.

    PubMed

    Kaufman, J D; Kassube, K R; Ríus, A G

    2017-10-01

    The objective of this study was to examine the effect of reducing rumen-degradable protein (RDP) and rumen-undegradable protein (RUP) proportions on feed intake, milk production, and N-use efficiency in primiparous and multiparous cows exposed to warm climates. Eighteen primiparous and 30 multiparous mid-lactation Holstein cows were used in a completely randomized design with a 2 × 2 factorial arrangement of treatments. Cows were randomly assigned to 1 of 4 dietary treatments formulated to contain 2 proportions of RDP (10 and 8%) and 2 proportions RUP (8 and 6%) of dry matter (DM) indicated as follows: (1) 10% RDP, 8% RUP; (2) 8% RDP, 8% RUP; (3) 10% RDP, 6% RUP; and (4) 8% RDP, 6% RUP. Protein sources were manipulated to obtain desired RDP and RUP proportions. Diets were isoenergetic and contained 50% forage and 50% concentrate (DM basis). Cows were individually fed the 10% RDP, 8% RUP diet 3 wk before treatment allocation. Cows were exposed to the prevailing Tennessee July and August temperature and humidity in a freestall barn with no supplemental cooling. Main effects and their interaction were tested using the Mixed procedure of SAS (least squares means ± standard error of the mean; SAS Institute Inc., Cary, NC). Observed values of nutrient intake and milk production were used to obtain NRC (2001) model predictions. Cows showed signs of heat stress throughout the study. Reducing from 10 to 8% RDP decreased dry matter intake (DMI; 0.9 kg/d) at 8% RUP, but increased DMI (2.6 kg/d) at 6% RUP in primiparous cows. Reducing from 10 to 8% RDP decreased milk yield (10%) at 8% RUP, but increased yield (14%) at 6% RUP. Treatments did not affect yield of energy-corrected milk. For multiparous cows, treatments did not affect DMI. Reducing from 10 to 8% RDP decreased yield of energy-corrected milk (3.4%) at 8% RUP, but increased yield (8.8%) at 6% RUP. Reducing from 10 to 8% RDP and 8 to 6% RUP both increased N-use efficiency for primiparous and multiparous cows. The NRC

  4. Crop yield response to increasing biochar rates

    USDA-ARS?s Scientific Manuscript database

    The benefit or detriment to crop yield from biochar application varies with biochar type/rate, soil, crop, or climate. The objective of this research was to identify yield response of cotton (Gossypium hirsutum L.), corn (Zea mayes L.), and peanut (Arachis hypogaea L.) to hardwood biochar applied at...

  5. Marker assisted pyramiding of drought yield QTLs into a popular Malaysian rice cultivar, MR219.

    PubMed

    Shamsudin, Noraziyah Abd Aziz; Swamy, B P Mallikarjuna; Ratnam, Wickneswari; Sta Cruz, Ma Teressa; Raman, Anitha; Kumar, Arvind

    2016-01-27

    Three drought yield QTLs, qDTY 2.2, qDTY 3.1, and qDTY 12.1 with consistent effect on grain yield under reproductive stage drought stress were pyramided through marker assisted breeding with the objective of improving the grain yield of the elite Malaysian rice cultivar MR219 under reproductive stage drought stress. Foreground selection using QTL specific markers, recombinant selection using flanking markers, and background selection were performed. BC1F3-derived lines with different combinations of qDTY 2.2 , qDTY 3.1, and qDTY 12.1 were evaluated under both reproductive stage drought stress and non-stress during the dry seasons of 2013 and 2014 at IRRI. The grain yield reductions in the stress trials compared to non-stress trials ranged from 79 to 93%. In the stress trials, delay in days to flowering and reduction in plant height were observed. In both seasons, MR219 did not produce any yield under stress, however it produced a yield of 5917 kg ha(-1) during the 2013 dry season and 8319 kg ha(-1) during the 2014 dry season under non-stress. Selected introgressed lines gave a yield advantage of 903 to 2500 kg ha(-1) over MR219 under reproductive stage drought stress and a yield of more than 6900 kg ha(-1) under non-stress during the 2014 dry season. Among lines with single qDTY, lines carrying qDTY 2.2 provided a higher yield advantage under reproductive stage drought stress in the MR219 background. Two-qDTY combinations (qDTY 3.1+ qDTY 2.2 and qDTY 3.1+ qDTY 12.1) performed better than lines with three qDTY combinations, indicating the absence of positive interactions between the three qDTYs. We successfully developed drought-tolerant MR219 pyramided lines with a yield advantage of more than 1500 kg ha(-1). Differential yield advantages of different combinations of the qDTYs indicate a differential synergistic relationship among qDTYs. This is the first report on the successful effect of qDTYs in increasing the yield under drought in genetic backgrounds other

  6. Short-term complete submergence of rice at the tillering stage increases yield.

    PubMed

    Zhang, Yajie; Wang, Zhensheng; Li, Lei; Zhou, Qun; Xiao, Yao; Wei, Xing; Zhou, Mingyao

    2015-01-01

    Flooding is a major threat to agricultural production. Most studies have focused on the lower water storage limit in rice fields, whereas few studies have examined the upper water storage limit. This study aimed to explore the effect of waterlogging at the rice tillering stage on rice growth and yield. The early-ripening late japonica variety Yangjing 4227 was selected for this study. The treatments included different submergence depths (submergence depth/plant height: 1/2 (waist submergence), 2/3 (neck submergence), and 1/1 (complete submergence)) and durations (1, 3, and 5 d). The control group was treated with the conventional alternation of drying and wetting. The effects of waterlogging at the tillering stage on root characteristics, dry matter production, nitrogen and phosphorus accumulation, yield, yield components, and 1-aminocyclopropane-1-carboxylic acid synthase (ACS) gene expression were explored. Compared with the control group, the 1/1 group showed significant increases in yield, seed-setting rate, photosynthetically efficient leaf area, and OS-ACS3 gene expression after 1 d of submergence. The grain number per panicle, dry weight of the aboveground and belowground parts, and number of adventitious roots also increased. Correlation analysis revealed a significant positive correlation between the panicle number and nitrogen content; however, no significant correlation was found for phosphorus content. If a decrease in rice yield of less than 10% is acceptable, half, 2/3, and complete submergence of the plants can be performed at the tillering stage for 1-3 d; this treatment will increase the space available for rice field water management/control and will improve rainfall resource utilization.

  7. Difference in Yield and Physiological Features in Response to Drought and Salinity Combined Stress during Anthesis in Tibetan Wild and Cultivated Barleys

    PubMed Central

    Ahmed, Imrul Mosaddek; Cao, Fangbin; Zhang, Mian; Chen, Xianhong; Zhang, Guoping; Wu, Feibo

    2013-01-01

    Soil salinity and drought are the two most common and frequently co-occurring abiotic stresses constraining crop growth and productivity. Greenhouse pot experiments were conducted to investigate the tolerance potential and mechanisms of Tibetan wild barley genotypes (XZ5, drought-tolerant; XZ16, salinity/aluminum tolerant) during anthesis compared with salinity-tolerant cv CM72 in response to separate and combined stresses (D+S) of drought (4% soil moisture, D) and salinity (S). Under salinity stress alone, plants had higher Na+ concentrations in leaves than in roots and stems. Importantly, XZ5 and XZ16 had substantially increased leaf K+ concentrations; XZ16 was more efficient in restricting Na+ loading in leaf and maintained a lower leaf Na+/K+ ratio. Moreover, a significant decrease in cell membrane stability index (CMSI) and an increase in malondialdehyde (MDA) were accompanied by a dramatic decrease in total biomass under D+S treatment. We demonstrated that glycine-betaine and soluble sugars increased significantly in XZ5 and XZ16 under all stress conditions, along with increases in protease activity and soluble protein contents. Significant increases were seen in reduced ascorbate (ASA) and reduced glutathione (GSH) contents, and in activities of H+K+-, Na+K+-, Ca++Mg++-, total- ATPase, and antioxidant enzymes under D+S treatment in XZ5 and XZ16 compared to CM72. Compared with control, all stress treatments significantly reduced grain yield and 1000-grain weight; however, XZ5 and XZ16 were less affected than CM72. Our results suggest that high tolerance to D+S stress in XZ5 and XZ16 is closely related to the lower Na+/K+ ratio, and enhanced glycine-betaine and soluble protein and sugar contents, improved protease, ATPase activities and antioxidative capacity for scavenging reactive oxygen species during anthesis. These results may provide novel insight into the potential responses associated with increasing D+S stress in wild barley genotypes. PMID:24205003

  8. Groundwater subsidies and penalties to corn yield

    NASA Astrophysics Data System (ADS)

    Zipper, S. C.; Booth, E.; Loheide, S. P.

    2013-12-01

    Proper water management is critical to closing yield gaps (observed yield below potential yield) as global populations continue to expand. However, the impacts of shallow groundwater on crop production and surface processes are poorly understood. The presence of groundwater within or just below the root zone has the potential to cause (via oxygen stress in poorly drained soils) or eliminate (via water supply in dry regions) yield gaps. The additional water use by a plant in the presence of shallow groundwater, compared to free drainage conditions, is called the groundwater subsidy; the depth at which the groundwater subsidy is greatest is the optimal depth to groundwater (DTGW). In wet years or under very shallow water table conditions, the groundwater subsidy is likely to be negative due to increased oxygen stress, and can be thought of as a groundwater penalty. Understanding the spatial dynamics of groundwater subsidies/penalties and how they interact with weather is critical to making sustainable agricultural and land-use decisions under a range of potential climates. Here, we examine patterns of groundwater subsidies and penalties in two commercial cornfields in the Yahara River Watershed, an urbanizing agricultural watershed in south-central Wisconsin. Water table levels are generally rising in the region due to a long-term trend of increasing precipitation over the last several decades. Biophysical indicators tracked throughout both the 2012 and 2013 growing seasons show a strong response to variable groundwater levels on a field scale. Sections of the field with optimal DTGW exhibit consistently higher stomatal conductance rates, taller canopies and higher leaf area index, higher ET rates, and higher pollination success rates. Patterns in these biophysical lines of evidence allow us to pinpoint specific periods within the growing season that plants were experiencing either oxygen or water stress. Most importantly, groundwater subsidies and penalties are

  9. Character-marked furniture: potential for lumber yield increase in rip-first rough mills

    Treesearch

    Urs Buehlmann; Janice K. Wiedenbeck; D. Earl Kline; D. Earl Kline

    1998-01-01

    The inclusion of character marks in furniture parts increases part yield at least as much as previously estimated by industrial practitioners and scientists specializing in yield efficiency. However, character-marked furniture is uncommon in the more popular North American furniture species and designs. Opportunities for extending the hardwood resource associated with...

  10. Impacts of extreme heat and drought on crop yields in China: an assessment by using the DLEM-AG2 model

    NASA Astrophysics Data System (ADS)

    Zhang, J.; Yang, J.; Pan, S.; Tian, H.

    2016-12-01

    China is not only one of the major agricultural production countries with the largest population in the world, but it is also the most susceptible to climate change and extreme events. Much concern has been raised about how extreme climate has affected crop yield, which is crucial for China's food supply security. However, the quantitative assessment of extreme heat and drought impacts on crop yield in China has rarely been investigated. By using the Dynamic Land Ecosystem Model (DLEM-AG2), a highly integrated process-based ecosystem model with crop-specific simulation, here we quantified spatial and temporal patterns of extreme climatic heat and drought stress and their impacts on the yields of major food crops (rice, wheat, maize, and soybean) across China during 1981-2015, and further investigated the underlying mechanisms. Simulated results showed that extreme heat and drought stress significantly reduced national cereal production and increased the yield gaps between potential yield and rain-fed yield. The drought stress was the primary factor to reduce crop yields in the semi-arid and arid regions, and extreme heat stress slightly aggravated the yield loss. The yield gap between potential yield and rain-fed yield was larger at locations with lower precipitation. Our results suggest that a large exploitable yield gap in response to extreme climatic heat-drought stress offers an opportunity to increase productivity in China by optimizing agronomic practices, such as irrigation, fertilizer use, sowing density, and sowing date.

  11. Character-marked furniture: potential for lumber yield increase in crosscut-first rough mills

    Treesearch

    Urs Buehlmann; Janice K. Wiedenbeck; E. Earl Kline; E. Earl Kline

    1999-01-01

    Including character marks in dimension parts of the furniture, cabinet, and dimension industries offers an opportunity to increase lumber yield substantially. However, little quantitative knowledge exists as to how the incorporation of character marks in parts influences yield when processing lumber in a crosscut-first rough mill. Using computer simulation, this study...

  12. Stress sensitizes the brain: increased processing of unpleasant pictures after exposure to acute stress.

    PubMed

    Weymar, Mathias; Schwabe, Lars; Löw, Andreas; Hamm, Alfons O

    2012-07-01

    A key component of acute stress is a surge in vigilance that enables a prioritized processing of highly salient information to promote the organism's survival. In this study, we investigated the neural effects of acute stress on emotional picture processing. ERPs were measured during a deep encoding task, in which 40 male participants categorized 50 unpleasant and 50 neutral pictures according to arousal and valence. Before picture encoding, participants were subjected either to the Socially Evaluated Cold Pressor Test (SECPT) or to a warm water control procedure. The exposure to the SECPT resulted in increased subjective and autonomic (heart rate and blood pressure) stress responses relative to the control condition. Viewing of unpleasant relative to neutral pictures evoked enhanced late positive potentials (LPPs) over centro-parietal scalp sites around 400 msec after picture onset. Prior exposure to acute stress selectively increased the LPPs for unpleasant pictures. Moreover, the LPP magnitude for unpleasant pictures following the SECPT was positively associated with incidental free recall performance 24 hr later. The present results suggest that acute stress sensitizes the brain for increased processing of cues in the environment, particularly priming the processing of unpleasant cues. This increased processing is related to later long-term memory performance.

  13. Perception matters: Stressful life events increase breast cancer risk.

    PubMed

    Fischer, Avital; Ziogas, Argyrios; Anton-Culver, Hoda

    2018-07-01

    The relationship between psychological stress and breast cancer risk is unclear. The present study sought to understand how stressfulness appraisal of salient Life Events (LEs) influences breast cancer risk. A case-control design was used and included 664 female cases identified through the Cancer Surveillance Program of Orange County, CA and 203 female population-based controls. A LE questionnaire determined if events occurred prior to breast cancer diagnosis and if these events were considered to be stressful or not. Multivariate unconditional logistic regression was used to calculate ORs while adjusting for known breast cancer covariates. Cumulative adverse LEs perceived as stressful were associated with increased breast cancer risk in a dose response fashion (OR = 1.63, 95% CI = 1.00-2.66, P trend  = 0.045). Conversely, events perceived as non-stressful did not have a significant impact on breast cancer risk. Previous personal illness was directly related to increased breast cancer risk, whether perceived as stressful (OR = 2.84, 95% CI = 1.96-4.11) or non-stressful (OR = 3.47, 95% CI = 1.34-8.94). Abortion and relocation were observed to have a protective effect on breast cancer risk only when reported as stressful (OR = 0.54, 95% CI = 0.32-0.92; OR = 0.63, 95% CI = 0.43-0.93, respectively). Pre/Peri-menopausal women who were nulliparous or who had their first child at ≥30 years of age were especially prone to the effects of appraised stress on increased breast cancer risk. This study underscores the importance of stressfulness appraisal when determining the effect of major LEs on breast cancer risk. Our results support incorporating assessments of perceived stressfulness in future epidemiological investigation of this topic. Copyright © 2018. Published by Elsevier Inc.

  14. [Effects of plastic mulch on soil moisture and temperature and limiting factors to yield increase for dryland spring maize in the North China].

    PubMed

    Liu, Sheng-Yao; Zhang, Li-Feng; Li, Zhi-Hong; Jia, Jian-Ming; Fan, Feng-Cui; Shi, Yu-Fang

    2014-11-01

    Four treatments, including ridge tillage with plastic mulch (RP), ridge tillage without mulch (RB), flat tillage with plastic mulch (FP) and flat tillage without mulch (FB), were carried out to examine the tillage type and mulch on the effects of soil moisture and temperature, yield and water use efficiency (WUE) of dry land spring maize in the North China. Results showed that the average soil temperature was increased by 1-3 °C and the accumulated soil temperature was increased by 155.2-280.9 °C from sowing to tasseling by plastic mulch, and the growing duration was extended by 5.9-10.7 d. The water conservation effect of plastic mulch was significant from sowing to the seedling establishment, with WUE being increased by 81.6%-136.4% under mulch as compared with that without mulch. From the seedling to jointing stage, which coincided with the dry period in the region, soil water utilization by the maize under mulch could reach the depth of 80-100 cm, and its WUE was about 17.0%-21.6% lower than the maize without mulch, since the latter was affected by dry stress. With the coming of rainy season around the trumpeting stage, soil water in each treatment was replenished and maintained at relative high level up to harvest. Yield of maize was increased by 9.5% under RP as compared with RB. However, yield was reduced by 5.0% under FP, due to the plastic film under flat tillage prevented the infiltration of rainfall and waterlogging occurred. No significant difference in yield was found between RB and FB. Higher yield of spring maize was limited because of the mismatching in water supply and demand characterized by soil water shortage before the rainy season and abundant soil water storage after the rainy season.

  15. Random regression models to account for the effect of genotype by environment interaction due to heat stress on the milk yield of Holstein cows under tropical conditions.

    PubMed

    Santana, Mário L; Bignardi, Annaiza Braga; Pereira, Rodrigo Junqueira; Menéndez-Buxadera, Alberto; El Faro, Lenira

    2016-02-01

    The present study had the following objectives: to compare random regression models (RRM) considering the time-dependent (days in milk, DIM) and/or temperature × humidity-dependent (THI) covariate for genetic evaluation; to identify the effect of genotype by environment interaction (G×E) due to heat stress on milk yield; and to quantify the loss of milk yield due to heat stress across lactation of cows under tropical conditions. A total of 937,771 test-day records from 3603 first lactations of Brazilian Holstein cows obtained between 2007 and 2013 were analyzed. An important reduction in milk yield due to heat stress was observed for THI values above 66 (-0.23 kg/day/THI). Three phases of milk yield loss were identified during lactation, the most damaging one at the end of lactation (-0.27 kg/day/THI). Using the most complex RRM, the additive genetic variance could be altered simultaneously as a function of both DIM and THI values. This model could be recommended for the genetic evaluation taking into account the effect of G×E. The response to selection in the comfort zone (THI ≤ 66) is expected to be higher than that obtained in the heat stress zone (THI > 66) of the animals. The genetic correlations between milk yield in the comfort and heat stress zones were less than unity at opposite extremes of the environmental gradient. Thus, the best animals for milk yield in the comfort zone are not necessarily the best in the zone of heat stress and, therefore, G×E due to heat stress should not be neglected in the genetic evaluation.

  16. Multigene manipulation of photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco

    PubMed Central

    Simkin, Andrew J.; McAusland, Lorna; Headland, Lauren R.; Lawson, Tracy; Raines, Christine A.

    2015-01-01

    Over the next 40 years it has been estimated that a 50% increase in the yield of grain crops such as wheat and rice will be required to meet the food and fuel demands of the increasing world population. Transgenic tobacco plants have been generated with altered combinations of sedoheptulose-1,7-bisphosphatase, fructose-1,6-bisphosphate aldolase, and the cyanobacterial putative-inorganic carbon transporter B, ictB, of which have all been identified as targets to improve photosynthesis based on empirical studies. It is shown here that increasing the levels of the three proteins individually significantly increases the rate of photosynthetic carbon assimilation, leaf area, and biomass yield. Furthermore, the daily integrated measurements of photosynthesis showed that mature plants fixed between 12–19% more CO2 than the equivalent wild-type plants. Further enhancement of photosynthesis and yield was observed when sedoheptulose-1,7-bisphosphatase, fructose-1,6-bisphosphate aldolase, and ictB were over-expressed together in the same plant. These results demonstrate the potential for the manipulation of photosynthesis, using multigene-stacking approaches, to increase crop yields. PMID:25956882

  17. Criteria for Yielding of Dispersion-Strengthened Alloys

    NASA Technical Reports Server (NTRS)

    Ansell, G. S.; Lenel, F. V.

    1960-01-01

    A dislocation model is presented in order to account for the yield behavior of alloys with a finely dispersed second-phase. The criteria for yielding used in the model, is that appreciable yielding occurs in these alloys when the shear stress due to piled-up groups of dislocations is sufficient to fracture or plastically deform the dispersed second-phase particles, relieving the back stress on the dislocation sources. Equations derived on the basis of this model, predict that the yield stress of the alloys varies as the reciprocal square root of the mean free path between dispersed particles. Experimental data is presented for several SAP-Type alloys, precipitation-hardened alloys and steels which are in good agreement with the yield strength variation as a function of dispersion spacing predicted by this theoretical treatment.

  18. Pad stress tests with increasing load for the diagnosis of stress urinary incontinence.

    PubMed

    Rimstad, Liv; Larsen, Elsa Skjønhaug; Schiøtz, Hjalmar A; Kulseng-Hanssen, Sigurd

    2014-09-01

    The aim of the study was to test the ability of pad stress tests with increasing load (supine, jumping on the floor, and jumping on a trampoline) to document stress incontinence in subjectively stress incontinent women. In this prospective study 147 subjectively stress and mixed incontinent women performed consecutively the three pad stress tests with a bladder volume of 300 ml. Nineteen women performed a second trampoline pad stress test to test repeatability of the test. Nine continent women performed a trampoline pad stress test in order to determine if subjectively continent women would leak during the test. Seventy-two women (49%) leaked during the supine, 136 (93%) leaked during the jumping, and 146 (99%) leaked during the trampoline pad stress test. The differences between pad stress tests were significant with P < 0.005. Correlation between the two trampoline pad stress tests was high at 0.8. None of the nine continent women leaked during the trampoline pad stress test. The supine pad stress test has low sensitivity and is therefore often falsely negative. The jumping pad stress test is a simple test to perform and is satisfactory for everyday use. Subjectively stress incontinent women who do not leak during the jumping pad stress test may perform a trampoline pad stress test to document stress incontinence. The trampoline pad stress test is also simple to perform and detected leakage in 91% of the women who did not leak during the jumping pad stress test. © 2013 Wiley Periodicals, Inc.

  19. Enhanced Sucrose Loading Improves Rice Yield by Increasing Grain Size.

    PubMed

    Wang, Liang; Lu, Qingtao; Wen, Xiaogang; Lu, Congming

    2015-12-01

    Yield in cereals is a function of grain number and size. Sucrose (Suc), the main carbohydrate product of photosynthesis in higher plants, is transported long distances from source leaves to sink organs such as seeds and roots. Here, we report that transgenic rice plants (Oryza sativa) expressing the Arabidopsis (Arabidopsis thaliana) phloem-specific Suc transporter (AtSUC2), which loads Suc into the phloem under control of the phloem protein2 promoter (pPP2), showed an increase in grain yield of up to 16% relative to wild-type plants in field trials. Compared with wild-type plants, pPP2::AtSUC2 plants had larger spikelet hulls and larger and heavier grains. Grain filling was accelerated in the transgenic plants, and more photoassimilate was transported from the leaves to the grain. In addition, microarray analyses revealed that carbohydrate, amino acid, and lipid metabolism was enhanced in the leaves and grain of pPP2::AtSUC2 plants. Thus, enhancing Suc loading represents a promising strategy to improve rice yield to feed the global population. © 2015 American Society of Plant Biologists. All Rights Reserved.

  20. Emotions and stress increase respiratory resistance in asthma.

    PubMed

    Ritz, T; Steptoe, A; DeWilde, S; Costa, M

    2000-01-01

    Clinical reports suggest that various emotions and types of stress can precipitate asthmatic symptoms, but there is little experimental evidence to substantiate this claim. We studied the impact of different emotional states and stress on respiratory resistance in asthmatic and nonasthmatic individuals. Participants (24 asthmatic and 24 nonasthmatic patients) viewed short film sequences selected to induce anxiety, anger, depression, elation, happiness, contentment, or a neutral affective state and completed two stressful tasks, mental arithmetic to induce active coping efforts and viewing of medical slides to induce passive coping efforts. Oscillatory resistance, heart rate, blood pressure, baroreflex sensitivity, skin conductance level, respiration rate and volume, and self-reported affective state were measured throughout the session. Uniform increases in oscillatory resistance were found in all emotional states compared with the neutral state and during mental arithmetic in both groups. Asthmatic patients showed stronger reactions to the medical slides than healthy control subjects, with significant increases in oscillatory resistance, blood pressure, skin conductance level, and minute volume, as well as higher levels of self-reported depression, arousal, and shortness of breath. Changes in oscillatory resistance were inconsistently correlated with other physiological indices. Various emotional states and stress increase oscillatory resistance largely independently of concurrent increases in autonomic or ventilatory activity. The particular sensitivity of asthmatics to passive coping demand requires additional research.

  1. Acute Stress Decreases but Chronic Stress Increases Myocardial Sensitivity to Ischemic Injury in Rodents

    PubMed Central

    Eisenmann, Eric D.; Rorabaugh, Boyd R.; Zoladz, Phillip R.

    2016-01-01

    Cardiovascular disease (CVD) is the largest cause of mortality worldwide, and stress is a significant contributor to the development of CVD. The relationship between acute and chronic stress and CVD is well evidenced. Acute stress can lead to arrhythmias and ischemic injury. However, recent evidence in rodent models suggests that acute stress can decrease sensitivity to myocardial ischemia–reperfusion injury (IRI). Conversely, chronic stress is arrhythmogenic and increases sensitivity to myocardial IRI. Few studies have examined the impact of validated animal models of stress-related psychological disorders on the ischemic heart. This review examines the work that has been completed using rat models to study the effects of stress on myocardial sensitivity to ischemic injury. Utilization of animal models of stress-related psychological disorders is critical in the prevention and treatment of cardiovascular disorders in patients experiencing stress-related psychiatric conditions. PMID:27199778

  2. Acute Stress Decreases but Chronic Stress Increases Myocardial Sensitivity to Ischemic Injury in Rodents.

    PubMed

    Eisenmann, Eric D; Rorabaugh, Boyd R; Zoladz, Phillip R

    2016-01-01

    Cardiovascular disease (CVD) is the largest cause of mortality worldwide, and stress is a significant contributor to the development of CVD. The relationship between acute and chronic stress and CVD is well evidenced. Acute stress can lead to arrhythmias and ischemic injury. However, recent evidence in rodent models suggests that acute stress can decrease sensitivity to myocardial ischemia-reperfusion injury (IRI). Conversely, chronic stress is arrhythmogenic and increases sensitivity to myocardial IRI. Few studies have examined the impact of validated animal models of stress-related psychological disorders on the ischemic heart. This review examines the work that has been completed using rat models to study the effects of stress on myocardial sensitivity to ischemic injury. Utilization of animal models of stress-related psychological disorders is critical in the prevention and treatment of cardiovascular disorders in patients experiencing stress-related psychiatric conditions.

  3. [Dynamics of Amomum villosum growth and its fruit yield cultivated under tropical forests].

    PubMed

    Zheng, Zheng; Gan, Jianmin; Feng, Zhili; Meng, Ying

    2004-01-01

    Investigations on the dynamics of Amomum villosum growth and its fruit yield cultivated under tropical ravine rainforest and secondary forest at different elevations in Xishuangbanna showed that the yield of A. villosum was influenced by the site age, sun light level of understorey, and water stress in dry season. The fruit yield and mature plant density decreased with increasing age of the A. villosum site. The fruit yield increased with sun light level when the light level in understorey was under 35% of full sun light (P < 0.05). The fruit yield at the lower site by stream was significantly higher than that at upper site (P < 0.05). The yield difference between ravine rainforest and secondary forest was not significant. Planned cultivation of A. villosum in the secondary forest of the shifting cultivation land by ravine from 800-1000 m elevation instead of customary cultivation in the ravine rainforest, could not only resolve the problem of the effect of light deficiency in understorey and water stress in the dry season on A. villosum fruit yield, but also be useful to protect the tropical ravine rain forest.

  4. Feeding sustains photosynthetic quantum yield of a scleractinian coral during thermal stress.

    PubMed

    Borell, Esther M; Bischof, Kai

    2008-10-01

    Thermal resistance of the coral-zooxanthellae symbiosis has been associated with chronic photoinhibition, increased antioxidant activity and protein repair involving high demands of nitrogen and energy. While the relative importance of heterotrophy as a source of nutrients and energy for cnidarian hosts, and as a means of nitrogen acquisition for their zooxanthellae, is well documented, the effect of feeding on the thermal sensitivity of the symbiotic association has been so far overlooked. Here we examine the effect of zooplankton feeding versus starvation on the bleaching susceptibility and photosynthetic activity of photosystem II (PSII) of zooxanthellae in the scleractinian coral Stylophora pistillata in response to thermal stress (daily temperature rises of 2-3 degrees C) over 10 days, employing pulse-amplitude-modulated chlorophyll fluorometry. Fed and starved corals displayed a decrease in daily maximum potential quantum yield (F (v)/F (m)) of PSII, effective quantum yield (F/F (m)') and relative electron transport rates over the course of 10 days. However after 10 days of exposure to elevated temperature, F (v)/F (m) of fed corals was still 50-70% higher than F (v)/F (m) of starved corals. Starved corals showed strong signs of chronic photoinhibition, which was reflected in a significant decline in nocturnal recovery rates of PSII relative to fed corals. This was paralleled by the progressive inability to dissipate excess excitation energy via non-photochemical quenching (NPQ). After 10 days, NPQ of starved corals had decreased by about 80% relative to fed corals. Feeding treatment had no significant effect on chlorophyll a and c (2) concentrations and zooxanthellae densities, but the mitotic indices were significantly lower in starved than in fed corals. Collectively the results indicate that exogenous food may reduce the photophysiological damage of zooxanthellae that typically leads to bleaching and could therefore play an important role in mediating the

  5. Hardening of particle/oil/water suspensions due to capillary bridges: Experimental yield stress and theoretical interpretation.

    PubMed

    Danov, Krassimir D; Georgiev, Mihail T; Kralchevsky, Peter A; Radulova, Gergana M; Gurkov, Theodor D; Stoyanov, Simeon D; Pelan, Eddie G

    2018-01-01

    Suspensions of colloid particles possess the remarkable property to solidify upon the addition of minimal amount of a second liquid that preferentially wets the particles. The hardening is due to the formation of capillary bridges (pendular rings), which connect the particles. Here, we review works on the mechanical properties of such suspensions and related works on the capillary-bridge force, and present new rheological data for the weakly studied concentration range 30-55 vol% particles. The mechanical strength of the solidified capillary suspensions, characterized by the yield stress Y, is measured at the elastic limit for various volume fractions of the particles and the preferentially wetting liquid. A quantitative theoretical model is developed, which relates Y with the maximum of the capillary-bridge force, projected on the shear plane. A semi-empirical expression for the mean number of capillary bridges per particle is proposed. The model agrees very well with the experimental data and gives a quantitative description of the yield stress, which increases with the rise of interfacial tension and with the volume fractions of particles and capillary bridges, but decreases with the rise of particle radius and contact angle. The quantitative description of capillary force is based on the exact theory and numerical calculation of the capillary bridge profile at various bridge volumes and contact angles. An analytical formula for Y is also derived. The comparison of the theoretical and experimental strain at the elastic limit reveals that the fluidization of the capillary suspension takes place only in a deformation zone of thickness up to several hundred particle diameters, which is adjacent to the rheometer's mobile plate. The reported experimental results refer to water-continuous suspension with hydrophobic particles and oily capillary bridges. The comparison of data for bridges from soybean oil and hexadecane surprisingly indicate that the yield strength is

  6. Preconception Alcohol Increases Offspring Vulnerability to Stress

    PubMed Central

    Jabbar, Shaima; Chastain, Lucy G; Gangisetty, Omkaram; Cabrera, Miguel A; Sochacki, Kamil; Sarkar, Dipak K

    2016-01-01

    The effect of preconception drinking by the mother on the life-long health outcomes of her children is not known, and therefore, in this study using an animal model, we determined the impact of preconception alcohol drinking of the mother on offspring stress response during adulthood. In our preconception alcohol exposure model, adult female rats were fed with 6.7% alcohol in their diet for 4 weeks, went without alcohol for 3 weeks and were bred to generate male and female offspring. Preconception alcohol-exposed offsprings' birth weight, body growth, stress response, anxiety-like behaviors, and changes in stress regulatory gene and protein hormone levels were evaluated. In addition, roles of epigenetic mechanisms in preconception alcohol effects were determined. Alcohol feeding three weeks prior to conception significantly affected pregnancy outcomes of female rats, with respect to delivery period and birth weight of offspring, without affecting maternal care behaviors. Preconception alcohol negatively affected offspring adult health, producing an increased stress hormone response to an immune challenge. In addition, preconception alcohol was associated with changes in expression and methylation profiles of stress regulatory genes in various brain areas. These changes in stress regulatory genes were normalized following treatment with a DNA methylation blocker during the postnatal period. These data highlight the novel possibility that preconception alcohol affects the inheritance of stress-related diseases possibly by epigenetic mechanisms. PMID:27296153

  7. Effects of physical agitation on yield of greenhouse-grown soybean

    NASA Technical Reports Server (NTRS)

    Jones, R. S.; Mitchell, C. A.

    1992-01-01

    Agronomic and horticultural crop species experience reductions in growth and harvestable yield after exposure to physical agitation (also known as mechanical stress), as by wind or rain. A greenhouse study was conducted to test the influence of mechanical stress on soybean yield and to determine if exposure to mechanical stress during discrete growth periods has differential effects on seed yield. A modified rotatory shaker was used to apply seismic (i.e., shaking) stress. Brief, periodic episodes of seismic stress reduced stem length, total seed dry weight, and seed number of soybean [Glycine max (L.) Merr.]. Lodging resistance was greater for plants stressed during vegetative growth or throughout vegetative and reproductive growth than during reproductive growth only. Seed dry weight yield was reduced regardless of the timing or duration of stress application, but was lowest when applied during reproductive development. Seismic stress applied during reproductive growth stages R1 to R2 (Days 3 to 4) was as detrimental to seed dry weight accumulation as was stress applied during growth stages R1 to R6 (Days 39 to 42). Seed dry weight per plant was highly correlated with seed number per plant, and seed number was correlated with the seed number of two- and three-seeded pods. Dry weight per 100 seeds was unaffected by seismic-stress treatment. Growth and yield reductions resulting from treatments applied only during the vegetative stage imply that long-term mechanical effects were induced, from which the plants did not fully recover. It is unclear which yield-controlling physiological processes were affected by mechanical stress. Both transient and long-term effects on yield-controlling processes remain to be elucidated.

  8. Genetic and management approaches to boost UK wheat yields by ameliorating water deficits.

    PubMed

    Dodd, Ian C; Whalley, W R; Ober, Eric S; Parry, M A J

    2011-11-01

    Faced with the challenge of increasing global food production, there is the need to exploit all approaches to increasing crop yields. A major obstacle to boosting yields of wheat (an important staple in many parts of the world) is the availability and efficient use of water, since there is increasing stress on water resources used for agriculture globally, and also in parts of the UK. Improved soil and crop management and the development of new genotypes may increase wheat yields when water is limiting. Technical and scientific issues concerning management options such as irrigation and the use of growth-promoting rhizobacteria are explored, since these may allow the more efficient use of irrigation. Fundamental understanding of how crops sense and respond to multiple abiotic stresses can help improve the effective use of irrigation water. Experiments are needed to test the hypothesis that modifying wheat root system architecture (by increasing root proliferation deep in the soil profile) will allow greater soil water extraction thereby benefiting productivity and yield stability. Furthermore, better knowledge of plant and soil interactions and how below-ground and above-ground processes communicate within the plant can help identify traits and ultimately genes (or alleles) that will define genotypes that yield better under dry conditions. Developing new genotypes will take time and, therefore, these challenges need to be addressed now.

  9. Stress does not increase blood–brain barrier permeability in mice

    PubMed Central

    Roszkowski, Martin

    2016-01-01

    Several studies have reported that exposure to acute psychophysiological stressors can lead to an increase in blood–brain barrier permeability, but these findings remain controversial and disputed. We thoroughly examined this issue by assessing the effect of several well-established paradigms of acute stress and chronic stress on blood–brain barrier permeability in several brain areas of adult mice. Using cerebral extraction ratio for the small molecule tracer sodium fluorescein (NaF, 376 Da) as a sensitive measure of blood–brain barrier permeability, we find that neither acute swim nor restraint stress lead to increased cerebral extraction ratio. Daily 6-h restraint stress for 21 days, a model for the severe detrimental impact of chronic stress on brain function, also does not alter cerebral extraction ratio. In contrast, we find that cold forced swim and cold restraint stress both lead to a transient, pronounced decrease of cerebral extraction ratio in hippocampus and cortex, suggesting that body temperature can be an important confounding factor in studies of blood–brain barrier permeability. To additionally assess if stress could change blood–brain barrier permeability for macromolecules, we measured cerebral extraction ratio for fluorescein isothiocyanate-dextran (70 kDa). We find that neither acute restraint nor cold swim stress affected blood–brain barrier permeability for macromolecules, thus corroborating our findings that various stressors do not increase blood–brain barrier permeability. PMID:27146513

  10. Yield Asymmetry Design of Magnesium Alloys by Integrated Computational Materials Engineering

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Li, Dongsheng; Joshi, Vineet V.; Lavender, Curt A.

    2013-11-01

    Deformation asymmetry of magnesium alloys is an important factor on machine design in automobile industry. Represented by the ratio of compressive yield stress (CYS) against tensile yield stress (TYS), deformation asymmetry is strongly related to microstructure, characterized by texture and grain size. Modified intermediate phi-model, a polycrystalline viscoplasticity model, is used to predict the deformation behavior of magnesium alloys with different grain sizes. Validated with experimental results, integrated computational materials engineering is applied to find out the route in achieving desired asymmetry by thermomechanical processing. In some texture, for example, rolled texture, CYS/TYS is smaller than 1 under different loadingmore » directions. In some texture, for example, extruded texture, asymmetry is large along normal direction. Starting from rolled texture, the asymmetry will increased to close to 1 along rolling direction after compressed to a strain of 0.2. Our model shows that grain refinement increases CYS/TYS. Besides texture control, grain refinement can also optimize the yield asymmetry. After the grain size decreased to a critical value, CYS/TYS reaches to 1 since CYS increases much faster than TYS. By tailoring the microstructure using texture control and grain refinement, it is achievable to optimize yield asymmetry in wrought magnesium alloys.« less

  11. Yield asymmetry design of magnesium alloys by integrated computational materials engineering

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Li, Dongsheng; Joshi, Vineet; Lavender, Curt

    2013-11-01

    Deformation asymmetry of magnesium alloys is an important factor on machine design in the automobile industry. Represented by the ratio of compressive yield stress (CYS) against tensile yield stress (TYS), deformation asymmetry is strongly related to texture and grain size. A polycrystalline viscoplasticity model, modified intermediate Φ-model, is used to predict the deformation behavior of magnesium alloys with different grain sizes. Validated with experimental results, integrated computational materials engineering is applied to find out the route in achieving desired asymmetry via thermomechanical processing. For example, CYS/TYS in rolled texture is smaller than 1 under different loading directions. In other textures,more » such as extruded texture, CYS/TYS is large along the normal direction. Starting from rolled texture, asymmetry will increase to close to 1 along the rolling direction after being compressed to a strain of 0.2. Our modified Φ-model also shows that grain refinement increases CYS/TYS. Along with texture control, grain refinement also can optimize the yield asymmetry. After the grain size decreases to a critical value, CYS/TYS reaches to 1 because CYS increases much faster than TYS. By tailoring the microstructure using texture control and grain refinement, it is achievable to optimize yield asymmetry in wrought magnesium alloys.« less

  12. TECHNICAL NOTE: The strengthening effect of guar gum on the yield stress of magnetorheological fluid

    NASA Astrophysics Data System (ADS)

    Wu, Wei Ping; Zhao, Bin Yuan; Wu, Qing; Chen, LeSheng; Hu, Ke Ao

    2006-08-01

    In this paper we present a novel approach for producing obvious strengthening of the magnetorheological (MR) effect of MR fluids. Carbonyl iron powders coated with guar gum were used as magnetic particles in the MR fluid. Experimental results showed that inducing a guar gum coating not only greatly improved the sedimentation stability but also strengthened the yield stress of the MR fluid. An intermolecular force based model was proposed for explaining the strengthening effect.

  13. Increased gluconeogenesis in rats exposed to hyper-G stress

    NASA Technical Reports Server (NTRS)

    Daligcon, B. C.; Oyama, J.; Hannak, K.

    1985-01-01

    The effect of gluconeogenesis on the levels of plasma glucose and liver glycogen was studied in rats exposed to hyper-G stress. Incorporation of lactate, alanine, or glycerol, labeled with C-14, into plasma glucose and liver glycogen was measured in rats centrifuged at 3.1 G for 0.25, 0.50, and 1.0-hr periods, and was compared to noncentrifuged controls injected with appropriate glycogen precursors. It was found that exposure to G-stress leads to increased incorporation from all three substrates into both plasma glucose and liver glycogen. These early incorporation increases were blocked upon pre-G administration of 5-methoxyindole-2-carboxylic acid, a gluconeogenesis inhibitor, or propanolol, a beta-adrenergic blocker, as well as by adrenodemedullation. Results indicate that the rapid rise in plasma glucose, as well as in liver glycogen in rats exposed to hyper-G stress is due to an increased rate of gluconeogenesis, and that epinephrine, released in response to hyper-G-induced activation of the sympathetic-adrenal system, plays a dominant role during the early stages of hyper-G stress.

  14. Yielding in a strongly aggregated colloidal gel: 2D simulations and theory

    NASA Astrophysics Data System (ADS)

    Roy, Saikat; Tirumkudulu, Mahesh

    2015-11-01

    We investigated the micro-structural details and the mechanical response under uniaxial compression of the strongly aggregating gel starting from low to high packing fraction.The numerical simulations account for short-range inter-particle attractions, normal and tangential deformation at particle contacts,sliding and rolling friction, and preparation history. It is observed that in the absence of rolling resistance(RR),the average coordination number varies only slightly with compaction whereas it is significant in the presence of RR. The particle contact distribution is isotropic throughout the consolidation process. In both cases, the yield strain is constant with the volume fraction. The modulus values are very similar at different attraction, and with and without RR implying that the elastic modulus does not scale with attraction.The modulus was found to be a weak function of the preparation history. The increase in yield stress with volume fraction is a consequence of the increased elastic modulus of the network. However, the yield stress scales similarly both with and without RR. The power law exponent of 5.4 is in good agreement with previous simulation results. A micromechanical theory is also proposed to describe the stress versus strain relation for the gelled network.

  15. Multiple yielding processes in a colloidal gel under large amplitude oscillatory stress

    NASA Astrophysics Data System (ADS)

    Gibaud, Thomas; Perge, Christophe; Lindström, Stefan B.; Taberlet, Nicolas; Manneville, Sébastien

    Fatigue refers to the changes in material properties caused by repeatedly applied loads. It has been widely studied for, e.g., construction materials, but much less has been done on soft materials. Here, we characterize the fatigue dynamics of a colloidal gel. Fatigue is induced by large amplitude oscillatory stress (LAOStress), and the local displacements of the gel are measured through high-frequency ultrasonic imaging. We show that fatigue eventually leads to rupture and fluidization. We evidence four successive steps associated with these dynamics: (i) the gel first remains solid, (ii) it then slides against the walls, (iii) the bulk of the sample becomes heterogeneous and displays solid-fluid coexistence, and (iv) it is finally fully fluidized. It is possible to homogeneously scale the duration of each step with respect to the stress oscillation amplitude $\\sigma_0$. The data are compatible with both exponential and power-law scalings with $\\sigma_0$, which hints at two possible interpretations in terms of delayed yielding in terms activated processes or of the Basquin law. Surprisingly, we find that the model parameters behave nonmonotonically as we change the oscillation frequency and/or the gel concentration.

  16. Overprotective social support leads to increased cardiovascular and subjective stress reactivity.

    PubMed

    Zniva, Richard; Pauli, Paul; Schulz, Stefan M

    2017-02-01

    Self-determination theory suggests that autonomy-enhancing social support helps individuals to perceive stressors as challenging rather than stressing. Overprotective support may reduce stress in the short-run but undermines autonomy, thus hampering stress-coping in the long run, particularly when social support is terminated. Heartrate, blood-pressure and ratings were examined in N=44 undergraduate students receiving autonomy support (calculation steps) or overprotection (solutions) from a close friend or no support for solving arithmetic tasks as well as during a subsequent stress-challenge (solving arithmetic tasks alone). Overprotection resulted in increased heartrate, diastolic blood-pressure, stress ratings, and decreased subjective control during stress-challenge. Autonomy support did not lead to unfavorable stress responding. The current findings are in line with assumptions derived from self-determination theory and indicate that autonomy support can help to prevent stress. Overprotection does not buffer stress and is associated with increased stress when discontinued. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Climate Based Predictability of Oil Palm Tree Yield in Malaysia.

    PubMed

    Oettli, Pascal; Behera, Swadhin K; Yamagata, Toshio

    2018-02-02

    The influence of local conditions and remote climate modes on the interannual variability of oil palm fresh fruit bunches (FFB) total yields in Malaysia and two major regions (Peninsular Malaysia and Sabah/Sarawak) is explored. On a country scale, the state of sea-surface temperatures (SST) in the tropical Pacific Ocean during the previous boreal winter is found to influence the regional climate. When El Niño occurs in the Pacific Ocean, rainfall in Malaysia reduces but air temperature increases, generating a high level of water stress for palm trees. As a result, the yearly production of FFB becomes lower than that of a normal year since the water stress during the boreal spring has an important impact on the total annual yields of FFB. Conversely, La Niña sets favorable conditions for palm trees to produce more FFB by reducing chances of water stress risk. The region of the Leeuwin current also seems to play a secondary role through the Ningaloo Niño/ Niña in the interannual variability of FFB yields. Based on these findings, a linear model is constructed and its ability to reproduce the interannual signal is assessed. This model has shown some skills in predicting the total FFB yield.

  18. Progranulin causes adipose insulin resistance via increased autophagy resulting from activated oxidative stress and endoplasmic reticulum stress.

    PubMed

    Guo, Qinyue; Xu, Lin; Li, Huixia; Sun, Hongzhi; Liu, Jiali; Wu, Shufang; Zhou, Bo

    2017-01-31

    Progranulin (PGRN) has recently emerged as an important regulator for insulin resistance. However, the direct effect of progranulin in adipose insulin resistance associated with the autophagy mechanism is not fully understood. In the present study, progranulin was administered to 3T3-L1 adipocytes and C57BL/6 J mice with/without specific inhibitors of oxidative stress and endoplasmic reticulum stress, and metabolic parameters, oxidative stress, endoplasmic reticulum stress and autophagy markers were assessed. Progranulin treatment increased iNOS expression, NO synthesis and ROS generation, and elevated protein expressions of CHOP, GRP78 and the phosphorylation of PERK, and caused a significant increase in Atg7 and LC3-II protein expression and a decreased p62 expression, and decreased insulin-stimulated tyrosine phosphorylation of IRS-1 and glucose uptake, demonstrating that progranulin activated oxidative stress and ER stress, elevated autophagy and induced insulin insensitivity in adipocytes and adipose tissue of mice. Interestingly, inhibition of iNOS and ER stress both reversed progranulin-induced stress response and increased autophagy, protecting against insulin resistance in adipocytes. Furthermore, the administration of the ER stress inhibitor 4-phenyl butyric acid reversed the negative effect of progranulin in vivo. Our findings showed the clinical potential of the novel adipokine progranulin in the regulation of insulin resistance, suggesting that progranulin might mediate adipose insulin resistance, at least in part, by inducing autophagy via activated oxidative stress and ER stress.

  19. Vacuum transfer system increases sugar maple sap yield

    Treesearch

    Russell S. Walters

    1978-01-01

    Yields of sugar maple sap collected from three plastic pipeline systems by gravity, vacuum pump, and a vacuum pump with a transfer tank were compared during 2 years in northern Vermont. The transfer system yielded 27 percent more sap one year and 17 percent more the next year. Higher vacuum levels at the tapholes were observed in the transfer system.

  20. Maternal stress during pregnancy increases neonatal allergy susceptibility: role of glucocorticoids.

    PubMed

    Lim, Robert; Fedulov, Alexey V; Kobzik, Lester

    2014-07-15

    We sought to test experimentally whether maternal stress can promote susceptibility to development of asthma-like allergic airways disease in offspring. Normal pregnant mice (day 15) were subjected to a single restraint stress exposure. We subsequently tested their offspring for the development of airway hyperreactivity (AHR) and allergic airway inflammation (AI), after an intentionally suboptimal sensitization protocol. The offspring of stressed mothers showed levels of AI and enhanced airway responses to methacholine comparable to those seen in fully sensitized and challenged positive control animals; in contrast, minimal effects were seen in control offspring. Restraint stress caused a rapid and large increase in plasma corticosterone levels. Maternal treatment with dexamethasone on day 15 of pregnancy mimicked the stress effect and reproduced the AI and AHR outcomes, whereas blockade of the stress-induced corticosterone surge with metyrapone pretreatment of pregnant mice abrogated the effect. We conclude that stress-triggered glucocorticoids during pregnancy can increase susceptibility to allergy in offspring. Because inflammation typically includes a stress hormone response, the results also suggest a common pathway by which various injurious exposures during pregnancy might increase offspring susceptibility to asthma. Copyright © 2014 the American Physiological Society.

  1. Plant-based assessment of inherent soil productivity and contributions to China's cereal crop yield increase since 1980.

    PubMed

    Fan, Mingsheng; Lal, Rattan; Cao, Jian; Qiao, Lei; Su, Yansen; Jiang, Rongfeng; Zhang, Fusuo

    2013-01-01

    China's food production has increased 6-fold during the past half-century, thanks to increased yields resulting from the management intensification, accomplished through greater inputs of fertilizer, water, new crop strains, and other Green Revolution's technologies. Yet, changes in underlying quality of soils and their effects on yield increase remain to be determined. Here, we provide a first attempt to quantify historical changes in inherent soil productivity and their contributions to the increase in yield. The assessment was conducted based on data-set derived from 7410 on-farm trials, 8 long-term experiments and an inventory of soil organic matter concentrations of arable land. Results show that even without organic and inorganic fertilizer addition crop yield from on-farm trials conducted in the 2000s was significantly higher compared with those in the 1980s - the increase ranged from 0.73 to 1.76 Mg/ha for China's major irrigated cereal-based cropping systems. The increase in on-farm yield in control plot since 1980s was due primarily to the enhancement of soil-related factors, and reflected inherent soil productivity improvement. The latter led to higher and stable yield with adoption of improved management practices, and contributed 43% to the increase in yield for wheat and 22% for maize in the north China, and, 31%, 35% and 22% for early and late rice in south China and for single rice crop in the Yangtze River Basin since 1980. Thus, without an improvement in inherent soil productivity, the 'Agricultural Miracle in China' would not have happened. A comprehensive strategy of inherent soil productivity improvement in China, accomplished through combining engineering-based measures with biological-approaches, may be an important lesson for the developing world. We propose that advancing food security in 21st century for both China and other parts of world will depend on continuously improving inherent soil productivity.

  2. Forage yield increased by clearcutting and site preparation

    Treesearch

    John J. Stransky; Lowell K. Halls

    1977-01-01

    Total forage yield (TFY) on a pine-hardwood forest site in east Texas was sampled before and 1 growing season after clearcutting (1972 and 1973), also 1 and 3 growing seasons after planting site preparation by burning, choping, or KG blading (1974 and 1976). Total forage yield was only 359 ka/ha in the uncut forest, but 2217 kg/ha after clearcutting. On control plots (...

  3. Shear stress increases nitric oxide production in thick ascending limbs

    PubMed Central

    Cabral, Pablo D.; Hong, Nancy J.

    2010-01-01

    We showed that luminal flow stimulates nitric oxide (NO) production in thick ascending limbs. Ion delivery, stretch, pressure, and shear stress all increase when flow is enhanced. We hypothesized that shear stress stimulates NO in thick ascending limbs, whereas stretch, pressure, and ion delivery do not. We measured NO in isolated, perfused rat thick ascending limbs using the NO-sensitive dye DAF FM-DA. NO production rose from 21 ± 7 to 58 ± 12 AU/min (P < 0.02; n = 7) when we increased luminal flow from 0 to 20 nl/min, but dropped to 16 ± 8 AU/min (P < 0.02; n = 7) 10 min after flow was stopped. Flow did not increase NO in tubules from mice lacking NO synthase 3 (NOS 3). Flow stimulated NO production by the same extent in tubules perfused with ion-free solution and physiological saline (20 ± 7 vs. 24 ± 6 AU/min; n = 7). Increasing stretch while reducing shear stress and pressure lowered NO generation from 42 ± 9 to 17 ± 6 AU/min (P < 0.03; n = 6). In the absence of shear stress, increasing pressure and stretch had no effect on NO production (2 ± 8 vs. 8 ± 8 AU/min; n = 6). Similar results were obtained in the presence of tempol (100 μmol/l), a O2− scavenger. Primary cultures of thick ascending limb cells subjected to shear stresses of 0.02 and 0.55 dyne/cm2 produced NO at rates of 55 ± 10 and 315 ± 93 AU/s, respectively (P < 0.002; n = 7). Pretreatment with the NOS inhibitor l-NAME (5 mmol/l) blocked the shear stress-induced increase in NO production. We concluded that shear stress rather than pressure, stretch, or ion delivery mediates flow-induced stimulation of NO by NOS 3 in thick ascending limbs. PMID:20719980

  4. Characteristics and interrelation of recovery stress and recovery strain of an ultrafine-grained Ni-50.2Ti alloy processed by high-ratio differential speed rolling

    NASA Astrophysics Data System (ADS)

    Lim, Y. G.; Kim, W. J.

    2017-03-01

    The characteristics of the recovery stress and strain of an ultrafine-grained Ni-50.2 at% Ti alloy prepared by high-ratio differential speed rolling (HRDSR) were examined, and the factors that influence the recovery stress and strain and the relation between the two were studied. After HRDSR, both the recovery stress and strain were enhanced compared to the initial condition. The subsequent annealing treatment at 673 K, however, reduced the shape recovery properties. The constitutive equation showing that the maximum recovery stress is a sole function of the recovery strain was developed. The recovery strain increased as the yield stress increased. Thus, the maximum recovery stress increased with an increase in yield stress. The recovery stress measured at room temperature (i.e., residual recovery stress) was, on the other hand, affected by the yield stress as well as the austenite-to-martensite transformation temperature. As the yield stress increased and as the martensitic transformation temperature decreased, the residual recovery stress increased.

  5. Attribution of maize yield increase in China to climate change and technological advancement between 1980 and 2010

    NASA Astrophysics Data System (ADS)

    Guo, Jianping; Zhao, Junfang; Wu, Dingrong; Mu, Jia; Xu, Yanhong

    2014-12-01

    Crop yields are affected by climate change and technological advancement. Objectively and quantitatively evaluating the attribution of crop yield change to climate change and technological advancement will ensure sustainable development of agriculture under climate change. In this study, daily climate variables obtained from 553 meteorological stations in China for the period 1961-2010, detailed observations of maize from 653 agricultural meteorological stations for the period 1981-2010, and results using an Agro-Ecological Zones (AEZ) model, are used to explore the attribution of maize (Zea mays L.) yield change to climate change and technological advancement. In the AEZ model, the climatic potential productivity is examined through three step-by-step levels: photosynthetic potential productivity, photosynthetic thermal potential productivity, and climatic potential productivity. The relative impacts of different climate variables on climatic potential productivity of maize from 1961 to 2010 in China are then evaluated. Combined with the observations of maize, the contributions of climate change and technological advancement to maize yield from 1981 to 2010 in China are separated. The results show that, from 1961 to 2010, climate change had a significant adverse impact on the climatic potential productivity of maize in China. Decreased radiation and increased temperature were the main factors leading to the decrease of climatic potential productivity. However, changes in precipitation had only a small effect. The maize yields of the 14 main planting provinces in China increased obviously over the past 30 years, which was opposite to the decreasing trends of climatic potential productivity. This suggests that technological advancement has offset the negative effects of climate change on maize yield. Technological advancement contributed to maize yield increases by 99.6%-141.6%, while climate change contribution was from -41.4% to 0.4%. In particular, the actual

  6. Photosynthesis and yield reductions from wheat stem sawfly (Hymenoptera: Cephidae): interactions with wheat solidness, water stress, and phosphorus deficiency.

    PubMed

    Delaney, Kevin J; Weaver, David K; Peterson, Robert K D

    2010-04-01

    The impact of herbivory on plants is variable and influenced by several factors. The current study examined causes of variation in the impact of larval stem mining by the wheat stem sawfly, Cephus cinctus Norton (Hymenoptera: Cephidae), on spring wheat, Triticum aestivum L. We performed greenhouse experiments over 2 yr to (1) study whether biotic (hollow versus solid stemmed host wheat) and abiotic (water, phosphorus stress) factors interact with C. cinctus stem mining to influence degree of mined stem physiological (photosynthesis) and yield (grain weight) reductions; and (2) determine whether whole plant yield compensatory responses occur to offset stem-mining reductions. Flag leaf photosynthetic reduction was not detected 16-20 d after infestation, but were detected at 40-42 d and doubled from water or phosphorus stresses. Main stem grain weight decreased from 10 to 25% from stem mining, largely due to reductions in grain size, with greater reductions under low phosphorus and/or water levels. Phosphorus-deficient plants without water stress were most susceptible to C. cinctus, more than doubling the grain weight reduction due to larval feeding relative to other water and phosphorus treatments. Two solid stemmed varieties with stem mining had less grain weight loss than a hollow stemmed variety, so greater internal mechanical resistance may reduce larval stem mining and plant yield reductions. Our results emphasize the importance of sufficient water and macronutrients for plants grown in regions impacted by C. cinctus. Also, solid stemmed varieties not only reduce wheat lodging from C. cinctus, they may reduce harvested grain losses from infested stems.

  7. The sunflower transcription factor HaWRKY76 confers drought and flood tolerance to Arabidopsis thaliana plants without yield penalty.

    PubMed

    Raineri, Jesica; Ribichich, Karina F; Chan, Raquel L

    2015-12-01

    Arabidopsis transgenic plants expressing the sunflower transcription factor HaWRKY76 exhibit increased yield and tolerance to drought and flood stresses. The genetic construct containing HaWRKY76 is proposed as a potential biotechnological tool to improve crops. Water deficit and water excess are abiotic stress factors that seriously affect crops worldwide. To increase the tolerance to such stresses without causing yield penalty constitutes a major goal for biotechnologists. In this survey, we report that HaWRKY76, a divergent sunflower WRKY transcription factor, is able to confer both dehydration and submergence tolerance to Arabidopsis transgenic plants without yield penalty. The expression pattern of HaWRKY76 was analyzed in plants grown in standard conditions and under different watering regimes indicating a regulation by water availability. The corresponding cDNA was isolated and cloned under the control of a constitutive promoter and Arabidopsis plants were transformed with this construct. These transgenic plants presented higher biomass, seed production and sucrose content than controls in standard growth conditions. Moreover, they exhibited tolerance to mild drought or flood (complete submergence/waterlogging) stresses as well as the same or increased yield, depending on the stress severity and plant developmental stage, compared with controls. Drought tolerance occurred via an ABA-independent mechanism and induction of stomatal closure. Submergence tolerance can be explained by the carbohydrate (sucrose and starch) preservation achieved through the repression of fermentation pathways. Higher cell membrane stability and chlorenchyma maintenance could be the nexus between tolerance responses in front of both stresses. Altogether, the obtained results indicated that HaWRKY76 can be a potential biotechnological tool to improve crops yield as well as drought and flood tolerances.

  8. Increased Fitness of Rice Plants to Abiotic Stress Via Habitat Adapted Symbiosis: A Strategy for Mitigating Impacts of Climate Change

    PubMed Central

    Redman, Regina S.; Kim, Yong Ok; Woodward, Claire J. D. A.; Greer, Chris; Espino, Luis; Doty, Sharon L.; Rodriguez, Rusty J.

    2011-01-01

    Climate change and catastrophic events have contributed to rice shortages in several regions due to decreased water availability and soil salinization. Although not adapted to salt or drought stress, two commercial rice varieties achieved tolerance to these stresses by colonizing them with Class 2 fungal endophytes isolated from plants growing across moisture and salinity gradients. Plant growth and development, water usage, ROS sensitivity and osmolytes were measured with and without stress under controlled conditions. The endophytes conferred salt, drought and cold tolerance to growth chamber and greenhouse grown plants. Endophytes reduced water consumption by 20–30% and increased growth rate, reproductive yield, and biomass of greenhouse grown plants. In the absence of stress, there was no apparent cost of the endophytes to plants, however, endophyte colonization decreased from 100% at planting to 65% compared to greenhouse plants grown under continual stress (maintained 100% colonization). These findings indicate that rice plants can exhibit enhanced stress tolerance via symbiosis with Class 2 endophytes, and suggest that symbiotic technology may be useful in mitigating impacts of climate change on other crops and expanding agricultural production onto marginal lands. PMID:21750695

  9. Increased fitness of rice plants to abiotic stress via habitat adapted symbiosis: A strategy for mitigating impacts of climate change

    USGS Publications Warehouse

    Redman, R.S.; Kim, Y.-O.; Woodward, C.J.D.A.; Greer, C.; Espino, L.; Doty, S.L.; Rodriguez, R.J.

    2011-01-01

    Climate change and catastrophic events have contributed to rice shortages in several regions due to decreased water availability and soil salinization. Although not adapted to salt or drought stress, two commercial rice varieties achieved tolerance to these stresses by colonizing them with Class 2 fungal endophytes isolated from plants growing across moisture and salinity gradients. Plant growth and development, water usage, ROS sensitivity and osmolytes were measured with and without stress under controlled conditions. The endophytes conferred salt, drought and cold tolerance to growth chamber and greenhouse grown plants. Endophytes reduced water consumption by 20–30% and increased growth rate, reproductive yield, and biomass of greenhouse grown plants. In the absence of stress, there was no apparent cost of the endophytes to plants, however, endophyte colonization decreased from 100% at planting to 65% compared to greenhouse plants grown under continual stress (maintained 100% colonization). These findings indicate that rice plants can exhibit enhanced stress tolerance via symbiosis with Class 2 endophytes, and suggest that symbiotic technology may be useful in mitigating impacts of climate change on other crops and expanding agricultural production onto marginal lands.

  10. Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory

    PubMed Central

    2010-01-01

    Background Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study we wanted to investigate whether our respiratory Saccharomyces cerevisiae strain, TM6*, could be used to enhance the productivity of recombinant proteins over that obtained from corresponding wild type, respiro-fermentative strains when cultured under the same laboratory conditions. Results Here we demonstrate at least a doubling in productivity over wild-type strains for three recombinant membrane proteins and one recombinant soluble protein produced in TM6* cells. In all cases, this was attributed to the improved biomass properties of the strain. The yield profile across the growth curve was also more stable than in a wild-type strain, and was not further improved by lowering culture temperatures. This has the added benefit that improved yields can be attained rapidly at the yeast's optimal growth conditions. Importantly, improved productivity could not be reproduced in wild-type strains by culturing them under glucose fed-batch conditions: despite having achieved very similar biomass yields to those achieved by TM6* cultures, the total volumetric yields were not concomitantly increased. Furthermore, the productivity of TM6* was unaffected by growing cultures in the presence of ethanol. These findings support the unique properties of TM6* as a microbial cell factory. Conclusions The accumulation of biomass in yeast cell factories is not necessarily correlated with a proportional increase in the functional yield of the recombinant protein being produced. The respiratory S. cerevisiae strain reported here is therefore a useful addition to the matrix of production hosts currently available as its improved biomass properties do lead to increased

  11. Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory.

    PubMed

    Ferndahl, Cecilia; Bonander, Nicklas; Logez, Christel; Wagner, Renaud; Gustafsson, Lena; Larsson, Christer; Hedfalk, Kristina; Darby, Richard A J; Bill, Roslyn M

    2010-06-17

    Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study we wanted to investigate whether our respiratory Saccharomyces cerevisiae strain, TM6*, could be used to enhance the productivity of recombinant proteins over that obtained from corresponding wild type, respiro-fermentative strains when cultured under the same laboratory conditions. Here we demonstrate at least a doubling in productivity over wild-type strains for three recombinant membrane proteins and one recombinant soluble protein produced in TM6* cells. In all cases, this was attributed to the improved biomass properties of the strain. The yield profile across the growth curve was also more stable than in a wild-type strain, and was not further improved by lowering culture temperatures. This has the added benefit that improved yields can be attained rapidly at the yeast's optimal growth conditions. Importantly, improved productivity could not be reproduced in wild-type strains by culturing them under glucose fed-batch conditions: despite having achieved very similar biomass yields to those achieved by TM6* cultures, the total volumetric yields were not concomitantly increased. Furthermore, the productivity of TM6* was unaffected by growing cultures in the presence of ethanol. These findings support the unique properties of TM6* as a microbial cell factory. The accumulation of biomass in yeast cell factories is not necessarily correlated with a proportional increase in the functional yield of the recombinant protein being produced. The respiratory S. cerevisiae strain reported here is therefore a useful addition to the matrix of production hosts currently available as its improved biomass properties do lead to increased volumetric yields without the need to

  12. Increasing family members' appreciation of family caregiving stress.

    PubMed

    Gopalan, Neena; Brannon, Laura A

    2006-03-01

    Participants read a message discussing the duties of a typical family caregiver (for example, a woman taking care of her elderly mother) and the associated psychological, physical, social, and financial stresses. The message was accompanied by an appeal that was either a control or was altruistic (other-oriented: focusing on the mother/caregiver's well-being) or egoistic (self-oriented: focusing on the child of the caregiver's needs and feelings). Participants who received an altruistic appeal were more appreciative of caregiving stresses than were those given an egoistic or a control appeal. Increasing family members' awareness of the stress involved in family caregiving is a first step in encouraging them to personally intervene on behalf of the caregiver.

  13. Increased endothelial microparticles and oxidative stress at extreme altitude.

    PubMed

    Pichler Hefti, Jacqueline; Leichtle, Alexander; Stutz, Monika; Hefti, Urs; Geiser, Thomas; Huber, Andreas R; Merz, Tobias M

    2016-04-01

    Hypoxia and oxidative stress affect endothelial function. Endothelial microparticles (MP) are established measures of endothelial dysfunction and influence vascular reactivity. To evaluate the effects of hypoxia and antioxidant supplementation on endothelial MP profiles, a double-blind, placebo-controlled trial, during a high altitude expedition was performed. 29 participants were randomly assigned to a treatment group (n = 14), receiving vitamin E, C, A, and N-acetylcysteine daily, and a control group (n = 15), receiving placebo. Blood samples were obtained at 490 m (baseline), 3530, 4590, and 6210 m. A sensitive tandem mass spectrometry method was used to measure 8-iso-prostaglandin F2α and hydroxyoctadecadienoic acids as markers of oxidative stress. Assessment of MP profiles including endothelial activation markers (CD62+MP and CD144+MP) and cell apoptosis markers (phosphatidylserine+MP and CD31+MP) was performed using a standardized flow cytometry-based protocol. 15 subjects reached all altitudes and were included in the final analysis. Oxidative stress increased significantly at altitude. No statistically significant changes were observed comparing baseline to altitude measurements of phosphatidylserine expressing MP (p = 0.1718) and CD31+MP (p = 0.1305). Compared to baseline measurements, a significant increase in CD62+MP (p = 0.0079) and of CD144+MP was detected (p = 0.0315) at high altitudes. No significant difference in any MP level or oxidative stress markers were found between the treatment and the control group. Hypobaric hypoxia is associated with increased oxidative stress and induces a significant increase in CD62+ and CD144+MP, whereas phosphatidylserine+MP and CD31+MP remain unchanged. This indicates that endothelial activation rather than an apoptosis is the primary factor of hypoxia induced endothelial dysfunction.

  14. Increased stress among women following an economic collapse--a prospective cohort study.

    PubMed

    Hauksdóttir, Arna; McClure, Christopher; Jonsson, Stefan Hrafn; Olafsson, Orn; Valdimarsdóttir, Unnur A

    2013-05-01

    There is a scarcity of data on mental health effects of the global economic recession. In this study, we investigated potential change in self-reported levels of psychological stress in the Icelandic population as a result of the major national economic collapse that occurred in 2008. We used a national cohort of 3,755 persons who responded to a survey administered in 2007 and 2009, including demographic questions and a stress measure (the 4-item Perceived Stress Scale). We used repeated-measures analysis of variance and logistic regression models to assess change in mean stress levels and risk of high stress levels (>90th percentile) in 2009 as compared with 2007. Age-adjusted mean stress levels increased between 2007 and 2009 (P = 0.004), though the increase was observed only for women (P = 0.003), not for men (P = 0.34). Similarly, the odds ratios for experiencing high stress levels were increased only among women (odds ratio (OR) = 1.37), especially among women who were unemployed (OR = 3.38), students (OR = 2.01), had middle levels of education (OR = 1.65), or were in the middle income bracket (OR = 1.59). The findings indicate that psychological stress may have increased following the economic collapse in Iceland, particularly among females in economically vulnerable groups.

  15. Constitutive expression of cell wall invertase genes increases grain yield and starch content in maize.

    PubMed

    Li, Bei; Liu, Hua; Zhang, Yue; Kang, Tao; Zhang, Li; Tong, Jianhua; Xiao, Langtao; Zhang, Hongxia

    2013-12-01

    Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive expression of cell wall invertase-encoding gene from Arabidopsis, rice (Oryza sativa) or maize (Zea mays), driven by the cauliflower mosaic virus (CaMV) 35S promoter, all increased cell wall invertase activities in different tissues and organs, including leaves and developing seeds, and substantially improved grain yield up to 145.3% in transgenic maize plants as compared to the wild-type plants, an effect that was reproduced in our 2-year field trials at different locations. The dramatically increased grain yield is due to the enlarged ears with both enhanced grain size and grain number. Constitutive expression of the invertase-encoding gene also increased total starch content up to 20% in the transgenic kernels. Our results suggest that cell wall invertase gene can be genetically engineered to improve both grain yield and grain quality in crop plants. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  16. Stress inducible overexpression of AtHDG11 leads to improved drought and salt stress tolerance in peanut (Arachis hypogaea L.)

    NASA Astrophysics Data System (ADS)

    Banavath, Jayanna N.; Chakradhar, Thammineni; Pandit, Varakumar; Konduru, Sravani; Guduru, Krishna K.; Akila, Chandra S.; Podha, Sudhakar; Puli, Chandra O. R.

    2018-03-01

    Peanut is an important oilseed and food legume cultivated as a rain-fed crop in semi-arid tropics. Drought and high salinity are the major abiotic stresses limiting the peanut productivity in this region. Development of drought and salt tolerant peanut varieties with improved yield potential using biotechnological approach is highly desirable to improve the peanut productivity in marginal geographies. As abiotic stress tolerance and yield represent complex traits, engineering of regulatory genes to produce abiotic stress-resilient transgenic crops appears to be a viable approach. In the present study, we developed transgenic peanut plants expressing an Arabidopsis homeodomain-leucine zipper transcription factor (AtHDG11) under stress inducible rd29Apromoter. A stress-inducible expression of AtHDG11 in three independent homozygous transgenic peanut lines resulted in improved drought and salt tolerance through up-regulation of known stress responsive genes(LEA, HSP70, Cu/Zn SOD, APX, P5CS, NCED1, RRS5, ERF1, NAC4, MIPS, Aquaporin, TIP, ELIP ) in the stress gene network , antioxidative enzymes, free proline along with improved water use efficiency traits such as longer root system, reduced stomatal density, higher chlorophyll content, increased specific leaf area, improved photosynthetic rates and increased intrinsic instantaneous WUE. Transgenic peanut plants displayed high yield compared to non-transgenic plants under both drought and salt stress conditions. Holistically, our study demonstrates the potentiality of stress-induced expression of AtHDG11 to improve the drought, salt tolerance in peanut.

  17. Thidiazuron (TDZ) increases fruit set and yield of 'Hosui' and 'Packham's Triumph' pear trees.

    PubMed

    Pasa, Mateus S; Silva, Carina P DA; Carra, Bruno; Brighenti, Alberto F; Souza, André Luiz K DE; Petri, José Luiz

    2017-01-01

    The low fruit set is one of the main factors leading to poor yield of pear orchards in Brazil. The exogenous application of thidiazuron (TDZ) and aminoethoxyvinilglycine (AVG) has shown promising results in some pear cultivars and other temperate fruit trees. The objective of this study was to evaluate the effect of TDZ and AVG on fruit set, yield, and fruit quality of 'Hosui' and 'Packham's Triumph' pears. The study was performed in a commercial orchard located in São Joaquim, SC. Plant material consisted of 'Hosui' and 'Packham's Triumph' pear trees grafted on Pyrus calleryana. Treatments consisted on different rates of TDZ (0 mg L-1, 20 mg L-1, 40 mg L-1 and 60 mg L-1) sprayed at full bloom for both cultivars. An additional treatment of AVG 60 mg L-1 was sprayed one week after full bloom in 'Hosui'. The fruit set, number of fruit per tree, yield, fruit weight, seed number, and fruit quality attributes were assessed. Fruit set and yield of both cultivars are consistently increased by TDZ, within the rates of 20 to 60 mg L-1. Besides, its application increased fruit size of 'Hosui' and did not negatively affect fruit quality attributes of both cultivars.

  18. Photon up-conversion increases biomass yield in Chlorella vulgaris.

    PubMed

    Menon, Kavya R; Jose, Steffi; Suraishkumar, Gadi K

    2014-12-01

    Photon up-conversion, a process whereby lower energy radiations are converted to higher energy levels via the use of appropriate phosphor systems, was employed as a novel strategy for improving microalgal growth and lipid productivity. Photon up-conversion enables the utilization of regions of the solar spectrum, beyond the typical photosynthetically active radiation, that are usually wasted or are damaging to the algae. The effects of up-conversion of red light by two distinct sets of up-conversion phosphors were studied in the model microalgae Chlorella vulgaris. Up-conversion by set 1 phosphors led to a 2.85 fold increase in biomass concentration and a 3.2 fold increase in specific growth rate of the microalgae. While up-conversion by set 2 phosphors resulted in a 30% increase in biomass and 12% increase in specific intracellular neutral lipid, while the specific growth rates were comparable to that of the control. Furthermore, up-conversion resulted in higher levels of specific intracellular reactive oxygen species in C. vulgaris. Up-conversion of red light (654 nm) was shown to improve biomass yields in C. vulgaris. In principle, up-conversion can be used to increase the utilization range of the electromagnetic spectrum for improved cultivation of photosynthetic systems such as plants, algae, and microalgae. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Analysis of a large dataset of mycorrhiza inoculation field trials on potato shows highly significant increases in yield.

    PubMed

    Hijri, Mohamed

    2016-04-01

    An increasing human population requires more food production in nutrient-efficient systems in order to simultaneously meet global food needs while reducing the environmental footprint of agriculture. Arbuscular mycorrhizal fungi (AMF) have the potential to enhance crop yield, but their efficiency has yet to be demonstrated in large-scale crop production systems. This study reports an analysis of a dataset consisting of 231 field trials in which the same AMF inoculant (Rhizophagus irregularis DAOM 197198) was applied to potato over a 4-year period in North America and Europe under authentic field conditions. The inoculation was performed using a liquid suspension of AMF spores that was sprayed onto potato seed pieces, yielding a calculated 71 spores per seed piece. Statistical analysis showed a highly significant increase in marketable potato yield (ANOVA, P < 0.0001) for inoculated fields (42.2 tons/ha) compared with non-inoculated controls (38.3 tons/ha), irrespective of trial year. The average yield increase was 3.9 tons/ha, representing 9.5 % of total crop yield. Inoculation was profitable with a 0.67-tons/ha increase in yield, a threshold reached in almost 79 % of all trials. This finding clearly demonstrates the benefits of mycorrhizal-based inoculation on crop yield, using potato as a case study. Further improvements of these beneficial inoculants will help compensate for crop production deficits, both now and in the future.

  20. Low pH increases the yield of exosome isolation.

    PubMed

    Ban, Jae-Jun; Lee, Mijung; Im, Wooseok; Kim, Manho

    2015-05-22

    Exosomes are the extracellular vesicles secreted by various cells. Exosomes mediate intercellular communication by delivering a variety of molecules between cells. Cancer cell derived exosomes seem to be related with tumor progression and metastasis. Tumor microenvironment is thought to be acidic and this low pH controls exosome physiology, leading to tumor progression. Despite the importance of microenvironmental pH on exosome, most of exosome studies have been performed without regard to pH. Therefore, the difference of exosome stability and yield of isolation by different pH need to be studied. In this research, we investigated the yield of total exosomal protein and RNA after incubation in acidic, neutral and alkaline conditioned medium. Representative exosome markers were investigated by western blot after incubation of exosomes in different pH. As a result, the concentrations of exosomal protein and nucleic acid were significantly increased after incubation in the acidic medium compared with neutral medium. The higher levels of exosome markers including CD9, CD63 and HSP70 were observed after incubation in an acidic environment. On the other hand, no exosomal protein, exosomal RNA and exosome markers have been detected after incubation in an alkaline condition. In summary, our results indicate that the acidic condition is the favorable environment for existence and isolation of exosomes. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Enhanced Sucrose Loading Improves Rice Yield by Increasing Grain Size1[OPEN

    PubMed Central

    Wang, Liang; Lu, Qingtao

    2015-01-01

    Yield in cereals is a function of grain number and size. Sucrose (Suc), the main carbohydrate product of photosynthesis in higher plants, is transported long distances from source leaves to sink organs such as seeds and roots. Here, we report that transgenic rice plants (Oryza sativa) expressing the Arabidopsis (Arabidopsis thaliana) phloem-specific Suc transporter (AtSUC2), which loads Suc into the phloem under control of the phloem protein2 promoter (pPP2), showed an increase in grain yield of up to 16% relative to wild-type plants in field trials. Compared with wild-type plants, pPP2::AtSUC2 plants had larger spikelet hulls and larger and heavier grains. Grain filling was accelerated in the transgenic plants, and more photoassimilate was transported from the leaves to the grain. In addition, microarray analyses revealed that carbohydrate, amino acid, and lipid metabolism was enhanced in the leaves and grain of pPP2::AtSUC2 plants. Thus, enhancing Suc loading represents a promising strategy to improve rice yield to feed the global population. PMID:26504138

  2. Lycopersicon esculentum under low temperature stress: an approach toward enhanced antioxidants and yield.

    PubMed

    Khan, Tanveer Alam; Fariduddin, Qazi; Yusuf, Mohammad

    2015-09-01

    Brassinosteroids (BRs) have been implicated to overcome various abiotic stresses, and low temperature stress poses a serious threat to productivity of various horticultural crops like tomato. Therefore, a study was conducted to unravel the possible role of BRs in conferring alleviation to low temperature stress in Lycopersicon esculentum. Twenty-day-old seedlings of tomato var. S-22 (chilling tolerant) and PKM-1 (chilling sensitive) were sown in earthen pots, and at 40 days stage of growth, plants were exposed to varied levels of low temperatures (10/3, 12/7, 20/14, or 25/18 °C) for 24 h in a growth chamber. At 50 days stage of growth, the foliage of plants were sprayed with 0 or 10(-8) M of BRs (28-homobrassinolide or 24-epibrassinolide), and 60-day-old plants were harvested to assess various physiological and biochemical parameters. Low temperatures induced a significant reduction in growth traits, chlorophyll content, and rate of photosynthesis in both the varieties differentially. Activities of antioxidant enzymes (catalase, peroxidase, and superoxide dismutase) and leaf proline content also increased substantially in both the varieties with decreasing temperature. On the other hand, treatment of BRs under stress and stress-free conditions significantly increased the aforesaid growth traits and biochemical parameters. Moreover, BRs further accelerated the antioxidative enzymes and proline content, which were already enhanced by the low temperature stress. Out of the two analogues of BRs tested, 24-epibrassinolide (EBL) was found more effective for both the varieties of tomato. EBL was found more potent stress alleviator against low temperature in both varieties of tomato.

  3. Parental stress and air pollution increase childhood asthma in China.

    PubMed

    Deng, Qihong; Deng, Linjing; Lu, Chan; Li, Yuguo; Norbäck, Dan

    2018-08-01

    Although air pollution and social stress may independently increase childhood asthma, little is known on their synergistic effect on asthma, particularly in China with high levels of stress and air pollution. To examine associations between exposure to a combination of parental stress and air pollution and asthma prevalence in children. We conducted a cohort study of 2406 preschool children in Changsha (2011-2012). A questionnaire was used to collect children's lifetime prevalence of asthma and their parental stress. Parental socioeconomic and psychosocial stresses were respectively defined in terms of housing size and difficulty concentrating. Children's exposure to ambient air pollutants was estimated using concentrations measured at monitoring stations. Associations between exposure to parental stress and air pollution and childhood asthma were estimated by multiple logistic regression models using odds ratio (OR) and 95% confidence interval (CI). Life time prevalence of asthma in preschool children (6.7%) was significantly associated with parental socioeconomic and psychosocial stresses with OR (95% CI) respectively 1.48 (1.02-2.16) and 1.64 (1.00-2.71). Asthma was also associated with exposure to air pollutants, with adjusted OR (95% CI) during prenatal and postnatal periods respectively 1.43 (1.10-1.86) and 1.35 (1.02-1.79) for SO 2 and 1.61 (1.19-2.18) and 1.76 (1.19-2.61) for NO 2 . The association with air pollution was significant only in children exposed to high parental stress, the association with parental stress was significant only in children exposed to high air pollution, and the association was the strongest in children exposed to a combination of parental stress and air pollution. Sensitivity analysis showed that the synergistic effects of parental stress and air pollution on childhood asthma were stronger in boys. Parental stress and air pollution were synergistically associated with increased childhood asthma, indicating a common biological

  4. Increased phytochrome B alleviates density effects on tuber yield of field potato crops.

    PubMed

    Boccalandro, Hernán E; Ploschuk, Edmundo L; Yanovsky, Marcelo J; Sánchez, Rodolfo A; Gatz, Christiane; Casal, Jorge J

    2003-12-01

    The possibility that reduced photomorphogenic responses could increase field crop yield has been suggested often, but experimental support is still lacking. Here, we report that ectopic expression of the Arabidopsis PHYB (phytochrome B) gene, a photoreceptor involved in detecting red to far-red light ratio associated with plant density, can increase tuber yield in field-grown transgenic potato (Solanum tuberosum) crops. Surprisingly, this effect was larger at very high densities, despite the intense reduction in the red to far-red light ratios and the concomitant narrowed differences in active phytochrome B levels between wild type and transgenics at these densities. Increased PHYB expression not only altered the ability of plants to respond to light signals, but they also modified the light environment itself. This combination resulted in larger effects of enhanced PHYB expression on tuber number and crop photosynthesis at high planting densities. The PHYB transgenics showed higher maximum photosynthesis in leaves of all strata of the canopy, and this effect was largely due to increased leaf stomatal conductance. We propose that enhanced PHYB expression could be used in breeding programs to shift optimum planting densities to higher levels.

  5. Polyelectrolyte scaling laws for microgel yielding near jamming.

    PubMed

    Bhattacharjee, Tapomoy; Kabb, Christopher P; O'Bryan, Christopher S; Urueña, Juan M; Sumerlin, Brent S; Sawyer, W Gregory; Angelini, Thomas E

    2018-02-28

    Micro-scale hydrogel particles, known as microgels, are used in industry to control the rheology of numerous different products, and are also used in experimental research to study the origins of jamming and glassy behavior in soft-sphere model systems. At the macro-scale, the rheological behaviour of densely packed microgels has been thoroughly characterized; at the particle-scale, careful investigations of jamming, yielding, and glassy-dynamics have been performed through experiment, theory, and simulation. However, at low packing fractions near jamming, the connection between microgel yielding phenomena and the physics of their constituent polymer chains has not been made. Here we investigate whether basic polymer physics scaling laws predict macroscopic yielding behaviours in packed microgels. We measure the yield stress and cross-over shear-rate in several different anionic microgel systems prepared at packing fractions just above the jamming transition, and show that our data can be predicted from classic polyelectrolyte physics scaling laws. We find that diffusive relaxations of microgel deformation during particle re-arrangements can predict the shear-rate at which microgels yield, and the elastic stress associated with these particle deformations predict the yield stress.

  6. Increased amygdala reactivity following early life stress: a potential resilience enhancer role.

    PubMed

    Yamamoto, Tetsuya; Toki, Shigeru; Siegle, Greg J; Takamura, Masahiro; Takaishi, Yoshiyuki; Yoshimura, Shinpei; Okada, Go; Matsumoto, Tomoya; Nakao, Takashi; Muranaka, Hiroyuki; Kaseda, Yumiko; Murakami, Tsuneji; Okamoto, Yasumasa; Yamawaki, Shigeto

    2017-01-18

    Amygdala hyper-reactivity is sometimes assumed to be a vulnerability factor that predates depression; however, in healthy people, who experience early life stress but do not become depressed, it may represent a resilience mechanism. We aimed to test these hypothesis examining whether increased amygdala activity in association with a history of early life stress (ELS) was negatively or positively associated with depressive symptoms and impact of negative life event stress in never-depressed adults. Twenty-four healthy participants completed an individually tailored negative mood induction task during functional magnetic resonance imaging (fMRI) assessment along with evaluation of ELS. Mood change and amygdala reactivity were increased in never-depressed participants who reported ELS compared to participants who reported no ELS. Yet, increased amygdala reactivity lowered effects of ELS on depressive symptoms and negative life events stress. Amygdala reactivity also had positive functional connectivity with the bilateral DLPFC, motor cortex and striatum in people with ELS during sad memory recall. Increased amygdala activity in those with ELS was associated with decreased symptoms and increased neural features, consistent with emotion regulation, suggesting that preservation of robust amygdala reactions may reflect a stress buffering or resilience enhancing factor against depression and negative stressful events.

  7. DD3MAT - a code for yield criteria anisotropy parameters identification.

    NASA Astrophysics Data System (ADS)

    Barros, P. D.; Carvalho, P. D.; Alves, J. L.; Oliveira, M. C.; Menezes, L. F.

    2016-08-01

    This work presents the main strategies and algorithms adopted in the DD3MAT inhouse code, specifically developed for identifying the anisotropy parameters. The algorithm adopted is based on the minimization of an error function, using a downhill simplex method. The set of experimental values can consider yield stresses and r -values obtained from in-plane tension, for different angles with the rolling direction (RD), yield stress and r -value obtained for biaxial stress state, and yield stresses from shear tests performed also for different angles to RD. All these values can be defined for a specific value of plastic work. Moreover, it can also include the yield stresses obtained from in-plane compression tests. The anisotropy parameters are identified for an AA2090-T3 aluminium alloy, highlighting the importance of the user intervention to improve the numerical fit.

  8. Grafting improves cucumber water stress tolerance in Saudi Arabia.

    PubMed

    Al-Harbi, Abdulaziz R; Al-Omran, Abdulrasoul M; Alharbi, Khadiga

    2018-02-01

    Water scarcity is a major limiting factor for crop productivity in arid and semi-arid areas. Grafting elite commercial cultivars onto selected vigorous rootstocks is considered as a useful strategy to alleviate the impact of environmental stresses. This study aims to investigate the feasibility of using grafting to improve fruit yield and quality of cucumber under water stress conditions. Alosama F 1 cucumber cultivar ( Cucumis sativus L.) was grafted onto Affyne ( Cucumis sativus L.) and Shintoza A90 ( Cucurbitamaxima × C. moschata ) rootstocks. Non-grafted plants were used as control. All genotypes were grown under three surface drip irrigation regimes: 50%, 75% and 100% of the crop evapotranspiration (ETc), which represent high-water stress, moderate-water stress and non-water stress conditions, respectively. Yield and fruit quality traits were analyzed and assessed. In comparison to the non-grafted plants, the best grafting treatment under water stress was Alosama F 1 grafted onto Shintoza A90 rootstock. It had an overall improved yield and fruit quality under water stress owing to an increase in the total fruit yield by 27%, from 4.815 kg plant -1 in non-grafted treatment to 6.149 kg plant -1 in grafted treatment under moderate -water stress, total soluble solid contents (13%), titratable acidity (39%) and vitamin C (33%). The soil water contents were low in soil surface and increase gradually with soil depth, while salt distribution showed an adverse trend. The positive effects of grafting on plant growth, productivity, and water use efficiency support this strategy as an useful tool for improving water stress tolerance in greenhouse grown cucumber in Saudi Arabia.

  9. More Yield with Less Water: Increasing Water Use Efficiency by Capitalizing on the Adaptation of Native Shrubs in the Sudano-Sahel

    NASA Astrophysics Data System (ADS)

    Bogie, Nathaniel; Bayala, Roger; Diedhiou, Ibrahima; Dick, Richard; Ghezzehei, Teamrat

    2016-04-01

    A changing climate along with human and animal population pressure can have a devastating effect on crop yields and food security in the Sudano-Sahel. Agricultural solutions to address soil degradation and crop water stress are needed to combat this increasingly difficult situation. Significant differences in crop success have been observed in peanut and millet grown in association with two native evergreen shrubs Piliostigma reticulatum, and Guiera senegalensis at the sites of Nioro du Rip and Keur Matar, respectively. We investigate how farmers can increase crop productivity by capitalizing on the evolutionary adaptation of native shrubs to the harsh Sudano-Sahelian environment as well as the physical mechanisms at work in the system that can lead to more robust yields. Research plots at Keur Matar Arame with no fertilizer added were monitored in 2013 using two soil moisture sensor networks at depths of 10, 20, 40, 60, 100, 200, and 300cm. Cropping season water use total calculated based on beginning and end of season soil moisture and seasonal precipitation data revealed that crop-only plot used 411±32 mm of water, and the crop and shrub plot used 439±42 mm of water. Taking into account the quantity of crop biomass produced and neglecting the shrub biomass produced, the crop and shrub plot had a water use efficiency of 1.60 kg ha-1 mm-1 and the crop only plot had 0.269 kg ha-1 mm-1. Water status was measured three times throughout the season on millet leaves and revealed no significant trends. Handheld NDVI readings revealed significantly higher NDVI values in crop and shrub plots at all measurement dates. These findings build on work that was completed in 2004 at the site, but further increases in crop yields have been shown. Increasing water use efficiency by over 500% can be a great advantage in years of limited water availability such as 2013. Using even the limited resources that farmers possess, this agroforestry technique can be expanded over wide

  10. High transpiration efficiency increases pod yield under intermittent drought in dry and hot atmospheric conditions but less so under wetter and cooler conditions in groundnut (Arachis hypogaea (L.)).

    PubMed

    Vadez, Vincent; Ratnakumar, Pasala

    2016-07-01

    Water limitation is a major yield limiting factor in groundnut and transpiration efficiency (TE) is considered the main target for improvement, but TE being difficult to measure it has mostly been screened with surrogates. The paper re-explore the contribution of TE to grain yield in peanut by using a novel experimental approach in which TE is measured gravimetrically throughout the crop life cycle, in addition to measurement of TE surrogates. Experimentation was carried out with the groundnut reference collection (n = 288), across seasons varying for the evaporative demand (vapor pressure deficit, VPD) and across both fully irrigated and intermittent water stress conditions. There was large genotypic variation for TE under water stress in both low and high VPD season but the range was larger (5-fold) in the high- than in the low-VPD season (2-fold). Under water stress in both seasons, yield was closely related to the harvest index (HI) while TE related directly to yield only in the high VPD season. After discounting the direct HI effect on yield, TE explained a large portion of the remaining yield variations in both seasons, although marginally in the low VPD season. By contrast, the total water extracted from the soil profile, which varied between genotypes, did not relate directly to pod yield and neither to the yield residuals unexplained by HI. Surrogates for TE (specific leaf area, SLA, and SPAD chlorophyll meter readings, SCMR) never showed any significant correlation to TE measurements. Therefore, TE is an important factor explaining yield differences in groundnut under high VPD environments, suggesting that stomatal regulation under high VPD, rather than high photosynthetic rate as proposed earlier, may have a key role to play in the large TE differences found, which open new opportunities to breed improved groundnut for high VPD.

  11. Heat-induced phytohormone changes are associated with disrupted early reproductive development and reduced yield in rice

    PubMed Central

    Wu, Chao; Cui, Kehui; Wang, Wencheng; Li, Qian; Fahad, Shah; Hu, Qiuqian; Huang, Jianliang; Nie, Lixiao; Peng, Shaobing

    2016-01-01

    Heat stress causes morphological and physiological changes and reduces crop yield in rice (Oryza sativa). To investigate changes in phytohormones and their relationships with yield and other attributes under heat stress, four rice varieties (Nagina22, Huanghuazhan, Liangyoupeijiu, and Shanyou 63) were grown in pots and subjected to three high temperature treatments plus control in temperature-controlled greenhouses for 15 d during the early reproductive phase. Yield reductions in Nagina22, Huanghuazhan, and Liangyoupeijiu were attributed to reductions in spikelet fertility, spikelets per panicle, and grain weight. The adverse effects of high temperature were alleviated by application of exogenous 6-benzylaminopurine (6-BA) in the heat-susceptible Liangyoupeijiu. High temperature stress reduced active cytokinins, gibberellin A1 (GA1), and indole-3-acetic acid (IAA), but increased abscisic acid (ABA) and bound cytokinins in young panicles. Correlation analyses and application of exogenous 6-BA revealed that high temperature-induced cytokinin changes may regulate yield components by modulating the differentiation and degradation of branches and spikelets, panicle exsertion, pollen vigor, anther dehiscence, and grain size. Heat-tolerant Shanyou 63 displayed minor changes in phytohormones, panicle formation, and grain yield under high temperature compared with those of the other three varieties. These results suggest that phytohormone changes are closely associated with yield formation, and a small reduction or stability in phytohormone content is required to avoid large yield losses under heat stress. PMID:27713528

  12. The Microstructural Evolution of Quartzite During Gradually Increasing Stress.

    NASA Astrophysics Data System (ADS)

    Soleymani, Hamid; Kidder, Steven B.; Hirth, Greg

    2016-12-01

    In settings where rocks are exhumed along shear zones, mylonites are thought to experience a gradual increase in stress and localization as they approach the brittle-ductile transition (Figure 1. left panel). Our aim is to investigate the microstructural characteristics of experimental samples that have experienced such a stress path and make comparisons to natural samples. A common characteristic of recrystallized grains in shear zones is what appears, at least qualitatively, to be a bimodal distribution of grain size (Figure 1. right panel). We hypothesize that such distributions might form as a natural consequence of a gradual stress increase in rocks approaching the brittle-ductile transition. We carried out several general-shear, Griggs rig experiments on Arkansas novaculite ( 10 micron grain size) and Black Hills quartzite synthesized powder (10-20 micron) annealed at 915°C and confining pressure of 1.5 GPa. To simulate exhumation, stress was increased by gradually decreasing the temperature at various constant rates. Experimental design and mechanical data are presented along with a discussion on grain growth and evolution. Initial results show that the technique is able to successfully simulate the exhumation stre­­­­ss path. The experiments also show that novaculite is roughly twice as strong (at similar water concentrations) as Black Hills quartzite powder ( 10-20 microns). We anticipate that detailed, quantitative study of the microstructure and grain statistics of experiments of this type can lead to improved interpretation of the microstructural development of natural samples.

  13. Climatically driven yield variability of major crops in Khakassia (South Siberia)

    NASA Astrophysics Data System (ADS)

    Babushkina, Elena A.; Belokopytova, Liliana V.; Zhirnova, Dina F.; Shah, Santosh K.; Kostyakova, Tatiana V.

    2018-06-01

    We investigated the variability of yield of the three main crop cultures in the Khakassia Republic: spring wheat, spring barley, and oats. In terms of yield values, variability characteristics, and climatic response, the agricultural territory of Khakassia can be divided into three zones: (1) the Northern Zone, where crops yield has a high positive response to the amount of precipitation, May-July, and a moderately negative one to the temperatures of the same period; (2) the Central Zone, where crops yield depends mainly on temperatures; and (3) the Southern Zone, where climate has the least expressed impact on yield. The dominant pattern in the crops yield is caused by water stress during periods of high temperatures and low moisture supply with heat stress as additional reason. Differences between zones are due to combinations of temperature latitudinal gradient, precipitation altitudinal gradient, and the presence of a well-developed hydrological network and the irrigational system as moisture sources in the Central Zone. More detailed analysis shows differences in the climatic sensitivity of crops during phases of their vegetative growth and grain development and, to a lesser extent, during harvesting period. Multifactor linear regression models were constructed to estimate climate- and autocorrelation-induced variability of the crops yield. These models allowed prediction of the possibility of yield decreasing by at least 2-11% in the next decade due to increasing of the regional summer temperatures.

  14. Climatically driven yield variability of major crops in Khakassia (South Siberia)

    NASA Astrophysics Data System (ADS)

    Babushkina, Elena A.; Belokopytova, Liliana V.; Zhirnova, Dina F.; Shah, Santosh K.; Kostyakova, Tatiana V.

    2017-12-01

    We investigated the variability of yield of the three main crop cultures in the Khakassia Republic: spring wheat, spring barley, and oats. In terms of yield values, variability characteristics, and climatic response, the agricultural territory of Khakassia can be divided into three zones: (1) the Northern Zone, where crops yield has a high positive response to the amount of precipitation, May-July, and a moderately negative one to the temperatures of the same period; (2) the Central Zone, where crops yield depends mainly on temperatures; and (3) the Southern Zone, where climate has the least expressed impact on yield. The dominant pattern in the crops yield is caused by water stress during periods of high temperatures and low moisture supply with heat stress as additional reason. Differences between zones are due to combinations of temperature latitudinal gradient, precipitation altitudinal gradient, and the presence of a well-developed hydrological network and the irrigational system as moisture sources in the Central Zone. More detailed analysis shows differences in the climatic sensitivity of crops during phases of their vegetative growth and grain development and, to a lesser extent, during harvesting period. Multifactor linear regression models were constructed to estimate climate- and autocorrelation-induced variability of the crops yield. These models allowed prediction of the possibility of yield decreasing by at least 2-11% in the next decade due to increasing of the regional summer temperatures.

  15. Estimating milk yield and value losses from increased somatic cell count on US dairy farms.

    PubMed

    Hadrich, J C; Wolf, C A; Lombard, J; Dolak, T M

    2018-04-01

    Milk loss due to increased somatic cell counts (SCC) results in economic losses for dairy producers. This research uses 10 mo of consecutive dairy herd improvement data from 2013 and 2014 to estimate milk yield loss using SCC as a proxy for clinical and subclinical mastitis. A fixed effects regression was used to examine factors that affected milk yield while controlling for herd-level management. Breed, milking frequency, days in milk, seasonality, SCC, cumulative months with SCC greater than 100,000 cells/mL, lactation, and herd size were variables included in the regression analysis. The cumulative months with SCC above a threshold was included as a proxy for chronic mastitis. Milk yield loss increased as the number of test days with SCC ≥100,000 cells/mL increased. Results from the regression were used to estimate a monetary value of milk loss related to SCC as a function of cow and operation related explanatory variables for a representative dairy cow. The largest losses occurred from increased cumulative test days with a SCC ≥100,000 cells/mL, with daily losses of $1.20/cow per day in the first month to $2.06/cow per day in mo 10. Results demonstrate the importance of including the duration of months above a threshold SCC when estimating milk yield losses. Cows with chronic mastitis, measured by increased consecutive test days with SCC ≥100,000 cells/mL, resulted in higher milk losses than cows with a new infection. This provides farm managers with a method to evaluate the trade-off between treatment and culling decisions as it relates to mastitis control and early detection. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  16. Early life stress increases testosterone and corticosterone and alters stress physiology in zebra finches.

    PubMed

    Zito, J Bayley; Hanna, Angy; Kadoo, Nora; Tomaszycki, Michelle L

    2017-09-01

    Early life stress has enduring effects on behavior and physiology. However, the effects on hormones and stress physiology remain poorly understood. In the present study, parents of zebra finches of both sexes were exposed to an increased foraging paradigm from 3 to 33days post hatching. Plasma and brains were collected from chicks at 3 developmental time points: post hatching days 25, 60 and adulthood. Plasma was assayed for testosterone (T), estradiol (E2), and corticosterone (CORT). The paraventricular nucleus of the hypothalamus was assessed for corticotrophin releasing factor (CRH) and glucocorticoid receptor (GR) expression. As expected, body mass was lower in nutritionally stressed animals compared to controls at multiple ages. Nutritionally stressed animals overall had higher levels of CORT than did control and this was particularly apparent in females at post hatching day 25. Nutritionally stressed animals also had a higher number of cells expressing CRH and GR in the paraventricular nucleus of the hypothalamus than did controls. There was an interaction, such that both measures were higher in control animals at PHD 25, but higher in NS animals by adulthood. Females, regardless of treatment, had higher circulating CORT and a higher number of cells expressing CRH than did males. Nutritionally stressed animals also had higher levels of T than did control animals, and this difference was greatest for males at post hatching day 60. There were no effects of nutritional stress on E2. These findings suggest that nutritional stress during development has long-lasting effects on testosterone and stress physiology. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Plant-Based Assessment of Inherent Soil Productivity and Contributions to China’s Cereal Crop Yield Increase since 1980

    PubMed Central

    Fan, Mingsheng; Lal, Rattan; Cao, Jian; Qiao, Lei; Su, Yansen; Jiang, Rongfeng; Zhang, Fusuo

    2013-01-01

    Objective China’s food production has increased 6-fold during the past half-century, thanks to increased yields resulting from the management intensification, accomplished through greater inputs of fertilizer, water, new crop strains, and other Green Revolution’s technologies. Yet, changes in underlying quality of soils and their effects on yield increase remain to be determined. Here, we provide a first attempt to quantify historical changes in inherent soil productivity and their contributions to the increase in yield. Methods The assessment was conducted based on data-set derived from 7410 on-farm trials, 8 long-term experiments and an inventory of soil organic matter concentrations of arable land. Results Results show that even without organic and inorganic fertilizer addition crop yield from on-farm trials conducted in the 2000s was significantly higher compared with those in the 1980s — the increase ranged from 0.73 to 1.76 Mg/ha for China’s major irrigated cereal-based cropping systems. The increase in on-farm yield in control plot since 1980s was due primarily to the enhancement of soil-related factors, and reflected inherent soil productivity improvement. The latter led to higher and stable yield with adoption of improved management practices, and contributed 43% to the increase in yield for wheat and 22% for maize in the north China, and, 31%, 35% and 22% for early and late rice in south China and for single rice crop in the Yangtze River Basin since 1980. Conclusions Thus, without an improvement in inherent soil productivity, the ‘Agricultural Miracle in China’ would not have happened. A comprehensive strategy of inherent soil productivity improvement in China, accomplished through combining engineering-based measures with biological-approaches, may be an important lesson for the developing world. We propose that advancing food security in 21st century for both China and other parts of world will depend on continuously improving inherent soil

  18. Increasing plant diversity with border crops reduces insecticide use and increases crop yield in urban agriculture

    PubMed Central

    Shen, Yan-Jun; Ji, Xiang-Yun; Wu, Xiang-Wen; Zheng, Xiang-Rong; Cheng, Wei; Li, Jun; Jiang, Yao-Pei; Chen, Xin; Weiner, Jacob; Nie, Ming; Ju, Rui-Ting; Yuan, Tao; Tang, Jian-Jun; Tian, Wei-Dong; Zhang, Hao

    2018-01-01

    Urban agriculture is making an increasing contribution to food security in large cities around the world. The potential contribution of biodiversity to ecological intensification in urban agricultural systems has not been investigated. We present monitoring data collected from rice fields in 34 community farms in mega-urban Shanghai, China, from 2001 to 2015, and show that the presence of a border crop of soybeans and neighboring crops (maize, eggplant and Chinese cabbage), both without weed control, increased invertebrate predator abundance, decreased the abundance of pests and dependence on insecticides, and increased grain yield and economic profits. Two 2 year randomized experiments with the low and high diversity practices in the same locations confirmed these results. Our study shows that diversifying farming practices can make an important contribution to ecological intensification and the sustainable use of associated ecosystem services in an urban ecosystem. PMID:29792597

  19. Increasing plant diversity with border crops reduces insecticide use and increases crop yield in urban agriculture.

    PubMed

    Wan, Nian-Feng; Cai, You-Ming; Shen, Yan-Jun; Ji, Xiang-Yun; Wu, Xiang-Wen; Zheng, Xiang-Rong; Cheng, Wei; Li, Jun; Jiang, Yao-Pei; Chen, Xin; Weiner, Jacob; Jiang, Jie-Xian; Nie, Ming; Ju, Rui-Ting; Yuan, Tao; Tang, Jian-Jun; Tian, Wei-Dong; Zhang, Hao; Li, Bo

    2018-05-24

    Urban agriculture is making an increasing contribution to food security in large cities around the world. The potential contribution of biodiversity to ecological intensification in urban agricultural systems has not been investigated. We present monitoring data collected from rice fields in 34 community farms in mega-urban Shanghai, China, from 2001 to 2015, and show that the presence of a border crop of soybeans and neighboring crops (maize, eggplant and Chinese cabbage), both without weed control, increased invertebrate predator abundance, decreased the abundance of pests and dependence on insecticides, and increased grain yield and economic profits. Two 2 year randomized experiments with the low and high diversity practices in the same locations confirmed these results. Our study shows that diversifying farming practices can make an important contribution to ecological intensification and the sustainable use of associated ecosystem services in an urban ecosystem. © 2018, Wan et al.

  20. Thermal stress effects in intermetallic matrix composites

    NASA Technical Reports Server (NTRS)

    Wright, P. K.; Sensmeier, M. D.; Kupperman, D. S.; Wadley, H. N. G.

    1993-01-01

    Intermetallic matrix composites develop residual stresses from the large thermal expansion mismatch (delta-alpha) between the fibers and matrix. This work was undertaken to: establish improved techniques to measure these thermal stresses in IMC's; determine residual stresses in a variety of IMC systems by experiments and modeling; and, determine the effect of residual stresses on selected mechanical properties of an IMC. X ray diffraction (XRD), neutron diffraction (ND), synchrotron XRD (SXRD), and ultrasonics (US) techniques for measuring thermal stresses in IMC were examined and ND was selected as the most promising technique. ND was demonstrated on a variety of IMC systems encompassing Ti- and Ni-base matrices, SiC, W, and Al2O3 fibers, and different fiber fractions (Vf). Experimental results on these systems agreed with predictions of a concentric cylinder model. In SiC/Ti-base systems, little yielding was found and stresses were controlled primarily by delta-alpha and Vf. In Ni-base matrix systems, yield strength of the matrix and Vf controlled stress levels. The longitudinal residual stresses in SCS-6/Ti-24Al-llNb composite were modified by thermomechanical processing. Increasing residual stress decreased ultimate tensile strength in agreement with model predictions. Fiber pushout strength showed an unexpected inverse correlation with residual stress. In-plane shear yield strength showed no dependence on residual stress. Higher levels of residual tension led to higher fatigue crack growth rates, as suggested by matrix mean stress effects.

  1. Genetic architecture of delayed senescence, biomass, and grain yield under drought stress in cowpea.

    PubMed

    Muchero, Wellington; Roberts, Philip A; Diop, Ndeye N; Drabo, Issa; Cisse, Ndiaga; Close, Timothy J; Muranaka, Satoru; Boukar, Ousmane; Ehlers, Jeffrey D

    2013-01-01

    The stay-green phenomenon is a key plant trait with wide usage in managing crop production under limited water conditions. This trait enhances delayed senescence, biomass, and grain yield under drought stress. In this study we sought to identify QTLs in cowpea (Vigna unguiculata) consistent across experiments conducted in Burkina Faso, Nigeria, Senegal, and the United States of America under limited water conditions. A panel of 383 diverse cowpea accessions and a recombinant inbred line population (RIL) were SNP genotyped using an Illumina 1536 GoldenGate assay. Phenotypic data from thirteen experiments conducted across the four countries were used to identify SNP-trait associations based on linkage disequilibrium association mapping, with bi-parental QTL mapping as a complementary strategy. We identified seven loci, five of which exhibited evidence suggesting pleiotropic effects (stay-green) between delayed senescence, biomass, and grain yield. Further, we provide evidence suggesting the existence of positive pleiotropy in cowpea based on positively correlated mean phenotypic values (0.34< r <0.87) and allele effects (0.07< r <0.86) for delayed senescence and grain yield across three African environments. Three of the five putative stay-green QTLs, Dro-1, 3, and 7 were identified in both RILs and diverse germplasm with resolutions of 3.2 cM or less for each of the three loci, suggesting that these may be valuable targets for marker-assisted breeding in cowpea. Also, the co-location of early vegetative delayed senescence with biomass and grain yield QTLs suggests the possibility of using delayed senescence at the seedling stage as a rapid screening tool for post-flowering drought tolerance in cowpea breeding. BLAST analysis using EST sequences harboring SNPs with the highest associations provided a genomic context for loci identified in this study in closely related common bean (Phaseolus vulgaris) and soybean (Glycine max) reference genomes.

  2. Increased dry season water yield in burned watersheds in Southern California

    NASA Astrophysics Data System (ADS)

    Kinoshita, Alicia M.; Hogue, Terri S.

    2015-01-01

    The current work evaluates the effects of the 2003 Old Fire on semi-arid systems in the San Bernardino Mountains, California. Pre- and post-fire daily streamflow are used to analyze flow regimes in two burned watersheds. The average pre-fire runoff ratios in Devil Canyon and City Creek are 0.14 and 0.26, respectively, and both increase to 0.34 post-fire. Annual flow duration curves are developed for each watershed and the low flow is characterized by a 90% exceedance probability threshold. Post-fire low flow is statistically different from the pre-fire values (α = 0.05). In Devil Canyon the annual volume of pre-fire low flow increases on average from 2.6E + 02 to 3.1E + 03 m3 (1090% increase) and in City Creek the annual low flow volume increases from 2.3E + 03 to 5.0E + 03 m3 (118% increase). Predicting burn system resilience to disturbance (anthropogenic and natural) has significant implications for water sustainability and ultimately may provide an opportunity to utilize extended and increased water yield.

  3. Increased bacterial cell density and recombinant protein yield using a commercial microbial cultivation system.

    PubMed

    Peck, Grantley R; Bowden, Timothy R; Shiell, Brian J; Michalski, Wojtek P

    2014-01-01

    EnBase (BioSilta, Finland) is a microbial cultivation system that replicates fed-batch systems through sustained release of glucose by enzymatic degradation of a polymeric substrate. Achievable bacterial cell densities and recombinant capripoxvirus protein expression levels, solubility, and antigenicity using the EnBase system were assessed. BL21-AI Escherichia coli expressing capripoxvirus proteins achieved up to eightfold higher cell densities when grown in EnBase media compared with standard media. Greater yields of capripoxvirus proteins were attained using EnBase media, either through increases in the amount of expressed protein per cell in conjunction with higher cell density or through the increase in cell density alone. Addition of EnBase booster enhanced protein yield for one of the proteins tested but reduced yield for the other. However, the amount of soluble forms of the capripoxvirus proteins tested was not different from that observed from cultures grown under standard conditions. Purified capripoxvirus proteins expressed using EnBase or standard media were assessed for their performance by enzyme-linked immunosorbent assay (ELISA) and were shown to be equally capable of specifically binding capripoxvirus antibodies.

  4. Duplication of an upstream silencer of FZP increases grain yield in rice.

    PubMed

    Bai, Xufeng; Huang, Yong; Hu, Yong; Liu, Haiyang; Zhang, Bo; Smaczniak, Cezary; Hu, Gang; Han, Zhongmin; Xing, Yongzhong

    2017-11-01

    Transcriptional silencer and copy number variants (CNVs) are associated with gene expression. However, their roles in generating phenotypes have not been well studied. Here we identified a rice quantitative trait locus, SGDP7 (Small Grain and Dense Panicle 7). SGDP7 is identical to FZP (FRIZZY PANICLE), which represses the formation of axillary meristems. The causal mutation of SGDP7 is an 18-bp fragment, named CNV-18bp, which was inserted ~5.3 kb upstream of FZP and resulted in a tandem duplication in the cultivar Chuan 7. The CNV-18bp duplication repressed FZP expression, prolonged the panicle branching period and increased grain yield by more than 15% through substantially increasing the number of spikelets per panicle (SPP) and slightly decreasing the 1,000-grain weight (TGW). The transcription repressor OsBZR1 binds the CGTG motifs in CNV-18bp and thereby represses FZP expression, indicating that CNV-18bp is the upstream silencer of FZP. These findings showed that the silencer CNVs coordinate a trade-off between SPP and TGW by fine-tuning FZP expression, and balancing the trade-off could enhance yield potential.

  5. High environmental stress yields greater tocotrienol content while changing vitamin e profiles of wild emmer wheat seeds.

    PubMed

    Watts, Emily J; Shen, Yu; Lansky, Ephraim P; Nevo, Eviatar; Bobe, Gerd; Traber, Maret G

    2015-02-01

    Vitamin E is an essential human nutrient that was first isolated from wheat. Emmer wheat, the cereal of Old World agriculture and a precursor to durum wheat, grows wild in the Fertile Crescent. Evolution Canyon, Israel, provides a microsite that models effects of contrasting environments. The north-facing and south-facing slopes exhibit low and high stress environments, respectively. Wild emmer wheat seeds were collected from both slopes and seed tocochromanol contents measured to test the hypothesis that high stress alters emmer wheat seed tocol-omics. Seeds from high stress areas contained more total vitamin E (108±15 nmol/g) than seeds from low stress environments (80±17 nmol/g, P=.0004). Vitamin E profiles within samples from these different environments revealed significant differences in isoform concentrations. Within each region, β- plus γ-tocotrienols represented the highest concentration of wheat tocotrienols (high stress, P<.0001; low stress, P<.0001), while α-tocopherol represented the highest concentration of the tocopherols (high stress, P=.0002; low stress, P<.0001). Percentages of both δ-tocotrienol and δ-tocopherol increased in high stress conditions. Changes under higher stress apparently are due to increased pathway flux toward more tocotrienol production. The production of more δ-isoforms suggests increased flow through a divergent path controlled by the VTE1 gene. Hence, stress conditions alter plant responses such that vitamin E profiles are changed, likely an attempt to provide additional antioxidant activity to promote seed viability and longevity.

  6. Induction of abiotic stress tolerance in plants by endophytic microbes.

    PubMed

    Lata, R; Chowdhury, S; Gond, S K; White, J F

    2018-04-01

    Endophytes are micro-organisms including bacteria and fungi that survive within healthy plant tissues and promote plant growth under stress. This review focuses on the potential of endophytic microbes that induce abiotic stress tolerance in plants. How endophytes promote plant growth under stressful conditions, like drought and heat, high salinity and poor nutrient availability will be discussed. The molecular mechanisms for increasing stress tolerance in plants by endophytes include induction of plant stress genes as well as biomolecules like reactive oxygen species scavengers. This review may help in the development of biotechnological applications of endophytic microbes in plant growth promotion and crop improvement under abiotic stress conditions. Increasing human populations demand more crop yield for food security while crop production is adversely affected by abiotic stresses like drought, salinity and high temperature. Development of stress tolerance in plants is a strategy to cope with the negative effects of adverse environmental conditions. Endophytes are well recognized for plant growth promotion and production of natural compounds. The property of endophytes to induce stress tolerance in plants can be applied to increase crop yields. With this review, we intend to promote application of endophytes in biotechnology and genetic engineering for the development of stress-tolerant plants. © 2018 The Society for Applied Microbiology.

  7. Food security: the challenge of increasing wheat yield and the importance of not compromising food safety

    PubMed Central

    Curtis, T; Halford, N G

    2014-01-01

    Current wheat yield and consumption is considered in the context of the historical development of wheat, from early domestication through to modern plant breeding, the Green Revolution and wheat’s place as one of the world’s most productive and important crops in the 21st Century. The need for further improvement in the yield potential of wheat in order to meet current and impending challenges is discussed, including rising consumption and the demand for grain for fuel as well as food. Research on the complex genetics underlying wheat yield is described, including the identification of quantitative trait loci and individual genes, and the prospects of biotechnology playing a role in wheat improvement in the future are discussed. The challenge of preparing wheat to meet the problems of drought, high temperature and increasing carbon dioxide concentration that are anticipated to come about as a result of climate change is also reviewed. Wheat yield must be increased while not compromising food safety, and the emerging problem of processing contaminants is reviewed, focussing in particular on acrylamide, a contaminant that forms from free asparagine and reducing sugars during high temperature cooking and processing. Wheat breeders are strongly encouraged to consider the contaminant issue when breeding for yield. PMID:25540461

  8. Grain size effect on yield strength of titanium alloy implanted with aluminum ions

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Popova, Natalya, E-mail: natalya-popova-44@mail.ru; Institute of Strength Physics and Materials Science, SB RAS, 2/4, Akademicheskii Ave., 634021, Tomsk; Nikonenko, Elena, E-mail: vilatomsk@mail.ru

    2016-01-15

    The paper presents a transmission electron microscopy (TEM) study of the microstructure and phase state of commercially pure titanium VT1-0 implanted by aluminum ions. This study has been carried out before and after the ion implantation for different grain size, i.e. 0.3 µm (ultra-fine grain condition), 1.5 µm (fine grain condition), and 17 µm (polycrystalline condition). This paper presents details of calculations and analysis of strength components of the yield stress. It is shown that the ion implantation results in a considerable hardening of the entire thickness of the implanted layer in the both grain types. The grain size has, however, a differentmore » effect on the yield stress. So, both before and after the ion implantation, the increase of the grain size leads to the decrease of the alloy hardening. Thus, hardening in ultra-fine and fine grain alloys increased by four times, while in polycrystalline alloy it increased by over six times.« less

  9. Bee pollination increases yield quantity and quality of cash crops in Burkina Faso, West Africa.

    PubMed

    Stein, Katharina; Coulibaly, Drissa; Stenchly, Kathrin; Goetze, Dethardt; Porembski, Stefan; Lindner, André; Konaté, Souleymane; Linsenmair, Eduard K

    2017-12-18

    Mutualistic biotic interactions as among flowering plants and their animal pollinators are a key component of biodiversity. Pollination, especially by insects, is a key element in ecosystem functioning, and hence constitutes an ecosystem service of global importance. Not only sexual reproduction of plants is ensured, but also yields are stabilized and genetic variability of crops is maintained, counteracting inbreeding depression and facilitating system resilience. While experiencing rapid environmental change, there is an increased demand for food and income security, especially in sub-Saharan communities, which are highly dependent on small scale agriculture. By combining exclusion experiments, pollinator surveys and field manipulations, this study for the first time quantifies the contribution of bee pollinators to smallholders' production of the major cash crops, cotton and sesame, in Burkina Faso. Pollination by honeybees and wild bees significantly increased yield quantity and quality on average up to 62%, while exclusion of pollinators caused an average yield gap of 37% in cotton and 59% in sesame. Self-pollination revealed inbreeding depression effects on fruit set and low germination rates in the F1-generation. Our results highlight potential negative consequences of any pollinator decline, provoking risks to agriculture and compromising crop yields in sub-Saharan West Africa.

  10. Increased gluconeogenesis in rats exposed to hyper-G stress

    NASA Technical Reports Server (NTRS)

    Daligcon, B. C.; Oyama, J.; Hannak, K.

    1985-01-01

    The effect of glucogenesis on the plasma glucose and liver glycogen of rats exposed to hyper-G stress is investigated. Twelve male Sprague-Dawley rats are injected with C-14 lactate, alanine, of glycerol, and six of the rats are exposed to 3.1 G for 0.25, 0.50, and 1.0 hr. The plasma glucose and liver glycogen of the centrifuged and noncentrifuged rats are analyzed. A significant increase in the C-14 incorporation of the substrate into the plasma glucose and liver glycogen is observed in the centrifuged rats. The injection of 5-methoxyindole-2-carboxylic acid, a gluconeogenesis inhibitor, results in a blocked increase in plasma glucose and liver glycogen. The role of epinephrine on the hyperglycemic and liver glycogen responses of centrifuged rats is studied. It is concluded that the initial increase in plasma glucose and liver glycogen in rats exposed to hyper-G stress is the result of an increased rate of gluconeogenesis.

  11. Post-Heading Heat Stress in Rice of South China during 1981-2010

    PubMed Central

    Shi, Peihua; Tang, Liang; Wang, Lihuan; Sun, Ting; Liu, Leilei; Cao, Weixing; Zhu, Yan

    2015-01-01

    Frequent extreme heat events are the serious threat to rice production, but the historical trend of heat stress associated with phenology shift and its impact on rice yield over a long period are poorly known. Based on the analysis of observed climate and phenology data from 228 stations in South China during 1981-2010, the spatio-temporal variation of post-heading heat stress was investigated among two single-season rice sub-regions in the northern Middle and Lower Reaches of Yangtze River (S-NMLYtz) and Southwest Plateau (S-SWP), and two double-season early rice sub-regions in the southern Middle and Lower Reaches of Yangtze River (DE-SMLYtz) and Southern China (DE-SC). Post-heading heat stress was more severe in DE-SMLYtz, west S-NMLYtz and east S-SWP than elsewhere, because of rice exposure to the hot season during post-heading stage. The spatial variation of post-heading heat stress was greater in single-season rice region than in double-season early rice region due to the greater spatial variation of heading and maturity dates. Post-heading heat stress increased from 1981 to 2010 in most areas, with significant increases in the east of double-season early rice region and west S-SWP. Phenology shift during 1981-2010 mitigated the increasing trends of heat stress in most areas, but not in west S-SWP. Post-heading heat stress played a dominated role in the reduction of rice yield in South China. Grain yield was more sensitive to post-heading heat stress in double-season early rice region than that in single-season rice region. Rice yield decreased by 1.5%, 6.2%, 9.7% and 4.6% in S-NMLYtz, S-SWP, DE-SMLYtz and DE-SC, respectively, because of post-heading heat stress during 1981-2010, although there were some uncertainties. Given the current level and potential increase of post-heading heat stress in South China, the specific adaptation or mitigation strategies are necessary for different sub-regions to stabilize rice production under heat stress. PMID:26110263

  12. Pyrolysis of polyolefins for increasing the yield of monomers' recovery

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Donaj, Pawel J., E-mail: pawel@mse.kth.se; Kaminsky, W.; Buzeto, F.

    2012-05-15

    Highlights: Black-Right-Pointing-Pointer Thermal and catalytic pyrolysis of mixed polyolefins in fluidized bed has been studied. Black-Right-Pointing-Pointer We tested applicability of a commercial Ziegler-Natta catalyst (Z-N: TiCl{sub 4}/MgCl{sub 2}). Black-Right-Pointing-Pointer The catalyst has a strong influence on product distribution, increasing gas fraction. Black-Right-Pointing-Pointer At 650 Degree-Sign C the monomer generation increased by 55% when the catalyst was used. Black-Right-Pointing-Pointer We showed the concept of treatment of mixed polyolefins without a need of separation. - Abstract: Pyrolysis of plastic waste is an alternative way of plastic recovery and could be a potential solution for the increasing stream of solid waste. The objectivemore » of this work was to increase the yield the gaseous olefins (monomers) as feedstock for polymerization process and to test the applicability of a commercial Ziegler-Natta (Z-N): TiCl{sub 4}/MgCl{sub 2} for cracking a mixture of polyolefins consisted of 46% wt. of low density polyethylene (LDPE), 30% wt. of high density polyethylene (HDPE) and 24% wt. of polypropylene (PP). Two sets of experiments have been carried out at 500 and 650 Degree-Sign C via catalytic pyrolysis (1% of Z-N catalyst) and at 650 and 730 Degree-Sign C via only-thermal pyrolysis. These experiments have been conducted in a lab-scale, fluidized quartz-bed reactor of a capacity of 1-3 kg/h at Hamburg University. The results revealed a strong influence of temperature and presence of catalyst on the product distribution. The ratios of gas/liquid/solid mass fractions via thermal pyrolysis were: 36.9/48.4/15.7% wt. and 42.4/44.7/13.9% wt. at 650 and 730 Degree-Sign C while via catalytic pyrolysis were: 6.5/89.0/4.5% wt. and 54.3/41.9/3.8% wt. at 500 and 650 Degree-Sign C, respectively. At 650 Degree-Sign C the monomer generation increased by 55% up to 23.6% wt. of total pyrolysis products distribution while the catalyst was added

  13. Increased Risk Taking in Relation to Chronic Stress in Adults

    PubMed Central

    Ceccato, Smarandita; Kudielka, Brigitte M.; Schwieren, Christiane

    2016-01-01

    Chronic stress is a public health problem that affects a significant part of the population. While the physiological damage it causes is under ongoing scrutiny, its behavioral effects have been overlooked. This is one of the first studies to examine the relation between chronic stress and decision-making, using a standard lottery paradigm. We measured risk taking in the gain domain through binary choices between financially incentivized lotteries. We then measured self-reported chronic stress with the Trier Inventory for the Assessment of Chronic Stress (TICS). We additionally collected hair samples in a subsample of volunteers, in order to quantify accumulation of the stress hormone cortisol. We discovered a significant positive, though modest, correlation between self-reported chronic stress and risk taking that is stronger for women than for men. This confirms part of the findings in acute stress research that show a connection between higher stress and increased risk taking. However, unlike the biologically-based results from acute stress research, we did not identify a significant relation between hair cortisol and behavior. In line with previous literature, we found a clear gender difference in risk taking and self-reports: women generally take less risk and report slightly higher stress levels than men. We conclude that perceived chronic stress can impact behavior in risky situations. PMID:26858663

  14. Optimising yield and resource utilisation of summer maize under the conditions of increasing density and reducing nitrogen fertilization.

    PubMed

    Wei, Shanshan; Wang, Xiangyu; Zhu, Qicen; Jiang, Dong; Dong, Shuting

    2017-10-05

    The inefficient use of resources always poses risks of maize (Zea mays L.) yield reduction in China. We performed this research to monitor the effects of increasing plant density and reducing nitrogen (N) rate on radiation-use efficiency (RUE), N efficiency traits, grain yield (GY) and their inter-relationships. Besides, whether GY and resource-use efficiency can both be maximized was examined. Hence, a 2-year field experiment was conducted using a widely grown variety "Denghai 618" in Shandong, China. Treatments contained two different plant densities [67,500 (D 1 ) and 97,500 (D 2 ) plant ha -1 ] and three N levels [0 (N -2 ), 180 (N -1 ), 360 (N ck ) kg ha -1 ], set D 1 N ck as control. Significant increases in grain yield, biomass, RUE, above-ground N uptake (AGN) and N efficiency were observed when density increased from D 1 to D 2 . Declining N application was accompanied by reductions in yield, RUE and AGN especially under high density, yet an obvious improvement in N recovery efficiency (NRE), agronomic N efficiency and N partial factor productivity. The increased GY was positive related with population biomass (r = 0.895**), RUE (r = 0.769**) and AGN (r = 0.923**), whereas it has no significant correlation with N efficiency. In this study, D 2 N ck obtained 18.8, 17.9, 24.8 and 29.7% higher grain yield, RUE, AGN and NRE respectively, compared to control, optimizing both yield and the efficiencies of radiation and N use. Furthermore, higher yield and RUE with more desirable N efficiency may be possible via optimizing density and N rate combination.

  15. Optimising yield and resource utilisation of summer maize under the conditions of increasing density and reducing nitrogen fertilization

    NASA Astrophysics Data System (ADS)

    Wei, Shanshan; Wang, Xiangyu; Zhu, Qicen; Jiang, Dong; Dong, Shuting

    2017-12-01

    The inefficient use of resources always poses risks of maize ( Zea mays L.) yield reduction in China. We performed this research to monitor the effects of increasing plant density and reducing nitrogen (N) rate on radiation-use efficiency (RUE), N efficiency traits, grain yield (GY) and their inter-relationships. Besides, whether GY and resource-use efficiency can both be maximized was examined. Hence, a 2-year field experiment was conducted using a widely grown variety "Denghai 618" in Shandong, China. Treatments contained two different plant densities [67,500 (D1) and 97,500 (D2) plant ha-1] and three N levels [0 (N-2), 180 (N-1), 360 (Nck) kg ha-1], set D1Nck as control. Significant increases in grain yield, biomass, RUE, above-ground N uptake (AGN) and N efficiency were observed when density increased from D1 to D2. Declining N application was accompanied by reductions in yield, RUE and AGN especially under high density, yet an obvious improvement in N recovery efficiency (NRE), agronomic N efficiency and N partial factor productivity. The increased GY was positive related with population biomass ( r = 0.895**), RUE ( r = 0.769**) and AGN ( r = 0.923**), whereas it has no significant correlation with N efficiency. In this study, D2Nck obtained 18.8, 17.9, 24.8 and 29.7% higher grain yield, RUE, AGN and NRE respectively, compared to control, optimizing both yield and the efficiencies of radiation and N use. Furthermore, higher yield and RUE with more desirable N efficiency may be possible via optimizing density and N rate combination.

  16. Acute stress induces increases in salivary IL-10 levels.

    PubMed

    Szabo, Yvette Z; Newton, Tamara L; Miller, James J; Lyle, Keith B; Fernandez-Botran, Rafael

    2016-09-01

    The purpose of this study was to investigate the stress-reactivity of the anti-inflammatory cytokine, IL-10, in saliva and to determine how salivary IL-10 levels change in relation to those of IL-1β, a pro-inflammatory cytokine, following stress. Healthy young adults were randomly assigned to retrieve a negative emotional memory (n = 46) or complete a modified version of the Trier Social Stress Test (n = 45). Saliva samples were taken 10 min before (baseline) and 50 min after (post-stressor) onset of a 10-min stressor, and were assayed using a high sensitivity multiplex assay for cytokines. Measurable IL-10 levels (above the minimum detectable concentration) were found in 96% of the baseline samples, and 98% of the post-stressor samples. Flow rate-adjusted salivary IL-10 levels as well as IL-1β/IL-10 ratios showed moderate but statistically significant increases in response to stress. Measurement of salivary IL-10 and pro-/anti-inflammatory cytokine ratios may be useful, noninvasive tools, in stress research.

  17. Effect of enzyme concentration, addition of water and incubation time on increase in yield of starch from potato.

    PubMed

    Sit, Nandan; Agrawal, U S; Deka, Sankar C

    2014-05-01

    Enzymatic treatment process for starch extraction from potato was investigated using cellulase enzyme and compared with conventional process. The effects of three parameters, cellulase enzyme concentration, incubation time and addition of water were evaluated for increase in starch yield as compared to the conventional process i.e., without using enzyme. A two-level full factorial design was used to study the process. The results indicated that all the main parameters and their interactions are statistically significant. Enzyme concentration and incubation time had a positive effect on the increase in starch yield while addition of water had a negative effect. The increase in starch yield ranged from 1.9% at low enzyme concentration and incubation time and high addition of water to a maximum of 70% increase from conventional process in starch yield was achieved when enzyme concentration and incubation time were high and addition of water was low suggesting water present in the ground potato meal is sufficient for access to the enzyme with in the slurry ensuring adequate contact with the substrate.

  18. Increasing plant density in eastern United States broccoli production systems to maximize marketable head yields

    USDA-ARS?s Scientific Manuscript database

    Increased demand for fresh market broccoli (Brassica oleracea L. var. italica) has led to increased production along the eastern seaboard of the United States. Maximizing broccoli yields is a primary concern for quickly expanding eastern commercial markets. Thus, a plant density study was carried ...

  19. Mapping QTLs for grain yield components in wheat under heat stress.

    PubMed

    Bhusal, Nabin; Sarial, Ashok Kumar; Sharma, Pradeep; Sareen, Sindhu

    2017-01-01

    The current perspective of increasing global temperature makes heat stress as a major threat to wheat production worldwide. In order to identify quantitative trait loci (QTLs) associated with heat tolerance, 251 recombinant inbred lines (RILs) derived from a cross between HD2808 (heat tolerant) and HUW510 (heat susceptible) were evaluated under timely sown (normal) and late sown (heat stress) conditions for two consecutive crop seasons; 2013-14 and 2014-15. Grain yield (GY) and its components namely, grain weight/spike (GWS), grain number/spike (GNS), thousand grain weight (TGW), grain filling rate (GFR) and grain filling duration (GFD) were recorded for both conditions and years. The data collected for both timely and late sown conditions and heat susceptibility index (HSI) of these traits were used as phenotypic data for QTL identification. The frequency distribution of HSI for all the studied traits was continuous during both the years and also included transgressive segregants. Composite interval mapping identified total 24 QTLs viz., 9 (timely sown traits), 6 (late sown traits) and 9 (HSI of traits) mapped on linkage groups 2A, 2B, and 6D during both the crop seasons 2013-14 and 2014-15. The QTLs were detected for GWS (6), GNS (6), GFR (4), TGW (3), GY (3) and GFD (2). The LOD score of identified QTLs varied from 3.03 (Qtgns.iiwbr-6D) to 21.01 (Qhsitgw.iiwbr-2A) during 2014-15, explaining 11.2 and 30.6% phenotypic variance, respectively. Maximum no of QTLs were detected in chromosome 2A followed by 6D and 2B. All the QTL detected under late sown and HSI traits were identified on chromosome 2A except for QTLs associated with GFD. Fifteen out of 17 QTL detected on chromosome 2A were clustered within the marker interval between gwm448 and wmc296 and showed tight linkage with gwm122 and these were localized in 49-52 cM region of Somers consensus map of chromosome 2A i.e. within 18-59.56 cM region of chromosome 2A where no QTL related to heat stress were reported

  20. Identification and mapping of stable QTL with main and epistasis effect on rice grain yield under upland drought stress

    PubMed Central

    2014-01-01

    Background Drought is one of the most important abiotic stresses that cause drastic reduction in rice grain yield (GY) in rainfed environments. The identification and introgression of QTL leading to high GY under drought have been advocated to be the preferred breeding strategy to improve drought tolerance of popular rice varieties. Genetic control of GY under reproductive-stage drought stress (RS) was studied in two BC1F4 mapping populations derived from crosses of Kali Aus, a drought-tolerant aus cultivar, with high-yielding popular varieties MTU1010 and IR64. The aim was to identify QTL for GY under RS that show a large and consistent effect for the trait. Bulk segregant analysis (BSA) was used to identify significant markers putatively linked with high GY under drought. Results QTL analysis revealed major-effect GY QTL: qDTY 1.2 , qDTY 2.2 and qDTY 1.3 , qDTY 2.3 (DTY; Drought grain yield) under drought consistently over two seasons in Kali Aus/2*MTU1010 and Kali Aus/2*IR64 populations, respectively. qDTY 1.2 and qDTY 2.2 explained an additive effect of 288 kg ha−1 and 567 kg ha−1 in Kali Aus/2*MTU1010, whereas qDTY 1.3 and qDTY 2.3 explained an additive effect of 198 kg ha−1 and 147 kg ha−1 in Kali Aus/2*IR64 populations, respectively. Epistatic interaction was observed for DTF (days to flowering) between regions on chromosome 2 flanked by markers RM154–RM324 and RM263–RM573 and major epistatic QTL for GY showing interaction between genomic locations on chromosome 1 at marker interval RM488–RM315 and chromosome 2 at RM324–RM263 in 2012 DS and 2013 DS RS in Kali Aus/2*IR64 mapping populations. Conclusion The QTL, qDTY 1.2 , qDTY 1.3 , qDTY 2.2 , and qDTY 2.3, identified in this study can be used to improve GY of mega varieties MTU1010 and IR64 under different degrees of severity of drought stress through marker-aided backcrossing and provide farmers with improved varieties that effectively combine high yield potential with good yield

  1. Developmental stress increases reproductive success in male zebra finches.

    PubMed

    Crino, Ondi L; Prather, Colin T; Driscoll, Stephanie C; Good, Jeffrey M; Breuner, Creagh W

    2014-11-22

    There is increasing evidence that exposure to stress during development can have sustained effects on animal phenotype and performance across life-history stages. For example, developmental stress has been shown to decrease the quality of sexually selected traits (e.g. bird song), and therefore is thought to decrease reproductive success. However, animals exposed to developmental stress may compensate for poor quality sexually selected traits by pursuing alternative reproductive tactics. Here, we examine the effects of developmental stress on adult male reproductive investment and success in the zebra finch (Taeniopygia guttata). We tested the hypothesis that males exposed to developmental stress sire fewer offspring through extra-pair copulations (EPCs), but invest more in parental care. To test this hypothesis, we fed nestlings corticosterone (CORT; the dominant avian stress hormone) during the nestling period and measured their adult reproductive success using common garden breeding experiments. We found that nestlings reared by CORT-fed fathers received more parental care compared with nestlings reared by control fathers. Consequently, males fed CORT during development reared nestlings in better condition compared with control males. Contrary to the prediction that developmental stress decreases male reproductive success, we found that CORT-fed males also sired more offspring and were less likely to rear non-genetic offspring compared with control males, and thus had greater overall reproductive success. These data are the first to demonstrate that developmental stress can have a positive effect on fitness via changes in reproductive success and provide support for an adaptive role of developmental stress in shaping animal phenotype. © 2014 The Author(s) Published by the Royal Society. All rights reserved.

  2. Measurements on stress dependent permeability

    NASA Astrophysics Data System (ADS)

    Risnes, R.; Faldaas, I.; Korsnes, R. I.; Norland, T.

    2003-04-01

    permeability are rather small, even if the tests are run beyond yield. The mid-section permeability seems to show a small increasing trend with increasing deviatoric stresses after yield. But the yield point does not seem to have any drastic effect on the permeability.(4) The overall permeability seems in general to show a decreasing trend under deviatoric stresses. The results indicate that permeability changes with pressure depletion under reservoir conditions may be much less than expected from hydrostatic tests or tests uncorrected for end-effects.

  3. Modern maize hybrids in Northeast China exhibit increased yield potential and resource use efficiency despite adverse climate change.

    PubMed

    Chen, Xiaochao; Chen, Fanjun; Chen, Yanling; Gao, Qiang; Yang, Xiaoli; Yuan, Lixing; Zhang, Fusuo; Mi, Guohua

    2013-03-01

    The impact of global changes on food security is of serious concern. Breeding novel crop cultivars adaptable to climate change is one potential solution, but this approach requires an understanding of complex adaptive traits for climate-change conditions. In this study, plant growth, nitrogen (N) uptake, and yield in relation to climatic resource use efficiency of nine representative maize cultivars released between 1973 and 2000 in China were investigated in a 2-year field experiment under three N applications. The Hybrid-Maize model was used to simulate maize yield potential in the period from 1973 to 2011. During the past four decades, the total thermal time (growing degree days) increased whereas the total precipitation and sunshine hours decreased. This climate change led to a reduction of maize potential yield by an average of 12.9% across different hybrids. However, the potential yield of individual hybrids increased by 118.5 kg ha(-1)  yr(-1) with increasing year of release. From 1973 to 2000, the use efficiency of sunshine hours, thermal time, and precipitation resources increased by 37%, 40%, and 41%, respectively. The late developed hybrids showed less reduction in yield potential in current climate conditions than old cultivars, indicating some adaptation to new conditions. Since the mid-1990s, however, the yield impact of climate change exhibited little change, and even a slight worsening for new cultivars. Modern breeding increased ear fertility and grain-filling rate, and delayed leaf senescence without modification in net photosynthetic rate. The trade-off associated with delayed leaf senescence was decreased grain N concentration rather than increased plant N uptake, therefore N agronomic efficiency increased simultaneously. It is concluded that modern maize hybrids tolerate the climatic changes mainly by constitutively optimizing plant productivity. Maize breeding programs in the future should pay more attention to cope with the limiting

  4. Genetic Improvements in Rice Yield and Concomitant Increases in Radiation- and Nitrogen-Use Efficiency in Middle Reaches of Yangtze River

    PubMed Central

    Zhu, Guanglong; Peng, Shaobing; Huang, Jianliang; Cui, Kehui; Nie, Lixiao; Wang, Fei

    2016-01-01

    The yield potential of rice (Oryza sativa L.) has experienced two significant growth periods that coincide with the introduction of semi-dwarfism and the utilization of heterosis. In present study, we determined the annual increase in the grain yield of rice varieties grown from 1936 to 2005 in Middle Reaches of Yangtze River and examined the contributions of RUE (radiation-use efficiency, the conversion efficiency of pre-anthesis intercepted global radiation to biomass) and NUE (nitrogen-use efficiency, the ratio of grain yield to aboveground N accumulation) to these improvements. An examination of the 70-year period showed that the annual gains of 61.9 and 75.3 kg ha−1 in 2013 and 2014, respectively, corresponded to an annual increase of 1.18 and 1.16% in grain yields, respectively. The improvements in grain yield resulted from increases in the harvest index and biomass, and the sink size (spikelets per panicle) was significantly enlarged because of breeding for larger panicles. Improvements were observed in RUE and NUE through advancements in breeding. Moreover, both RUE and NUE were significantly correlated with the grain yield. Thus, our study suggests that genetic improvements in rice grain yield are associated with increased RUE and NUE. PMID:26876641

  5. Response of Velocity Anisotropy of Shale Under Isotropic and Anisotropic Stress Fields

    NASA Astrophysics Data System (ADS)

    Li, Xiaying; Lei, Xinglin; Li, Qi

    2018-03-01

    We investigated the responses of P-wave velocity and associated anisotropy in terms of Thomsen's parameters to isotropic and anisotropic stress fields on Longmaxi shales cored along different directions. An array of piezoelectric ceramic transducers allows us to measure P-wave velocities along numerous different propagation directions. Anisotropic parameters, including the P-wave velocity α along a symmetry axis, Thomsen's parameters ɛ and δ, and the orientation of the symmetry axis, could then be extracted by fitting Thomsen's weak anisotropy model to the experimental data. The results indicate that Longmaxi shale displays weakly intrinsic velocity anisotropy with Thomsen's parameters ɛ and δ being approximately 0.05 and 0.15, respectively. The isotropic stress field has only a slight effect on velocity and associated anisotropy in terms of Thomsen's parameters. In contrast, both the magnitude and orientation of the anisotropic stress field with respect to the shale fabric are important in controlling the evolution of velocity and associated anisotropy in a changing stress field. For shale with bedding-parallel loading, velocity anisotropy is enhanced because velocities with smaller angles relative to the maximum stress increase significantly during the entire loading process, whereas those with larger angles increase slightly before the yield stress and afterwards decrease with the increasing differential stress. For shale with bedding-normal loading, anisotropy reversal is observed, and the anisotropy is progressively modified by the applied differential stress. Before reaching the yield stress, velocities with smaller angles relative to the maximum stress increase more significantly and even exceed the level of those with larger angles. After reaching the yield stress, velocities with larger angles decrease more significantly. Microstructural features such as the closure and generation of microcracks can explain the modification of the velocity anisotropy

  6. High pressure thermal hydrolysis as pre-treatment to increase the methane yield during anaerobic digestion of microalgae.

    PubMed

    Keymer, Philip; Ruffell, Ian; Pratt, Steven; Lant, Paul

    2013-03-01

    Anaerobic digestion of algal biomass will be an essential component of algal biofuel production systems, yet the methane yield from digestion of algae is typically much lower than the theoretical potential. In this work, high pressure thermal hydrolysis (HPTH) is shown to enhance methane yield during algae digestion. HPTH pre-treatment was applied to both raw algae and algal residue resulting from lipid extraction. HPTH and even the lipid extraction process itself increased methane yield, by 81% and 33% respectively; in combination they increased yield by 110% over that of the raw algae (18L CH4 gVS(-1) substrate). HPTH had little effect on the rate of anaerobic digestion, however lipid extraction enhanced it by 33% over that for raw algae (0.21day(-1)). Digestion resulted in solubilisation of nitrogen (and phosphorous to a lesser degree) in all cases, showing that there is potential for nutrient recycling for algal growth. Copyright © 2013 Elsevier Ltd. All rights reserved.

  7. Use of an Additional 19-G EBUS-TBNA Needle Increases the Diagnostic Yield of EBUS-TBNA.

    PubMed

    Garrison, Garth; Leclair, Timothy; Balla, Agnes; Wagner, Sarah; Butnor, Kelly; Anderson, Scott R; Kinsey, C Matthew

    2018-06-12

    Although endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) has an excellent diagnostic yield, there remain cases where the diagnosis is not obtained. We hypothesized that additional sampling with a 19-G EBUS-TBNA needle may increase diagnostic yield in a subset of cases where additional tissue sampling was required. Indications for use of the 19-G needle following 22-G sampling with rapid on-site cytologic examination were: (1) diagnostic uncertainty of the on-site cytopathologist (eg, nondiagnostic, probable lymphoma, etc.), (2) non-small cell lung cancer with probable need for molecular genetic and/or PD-L1 testing, or (3) need for a larger tissue sample for consideration of inclusion in a research protocol. A 19-G EBUS-TBNA needle was utilized following standard sampling with a 22-G needle in 48 patients (50 sites) during the same procedure. Although the diagnostic yield between the needles was equivalent, the concordance rate was only 83%. The 19-G determined a diagnosis in 4 additional patients (8%) and provided additional histopathologic information in 6 other cases (12%). Conversely, in 3 cases (6%) diagnostic information was provided only by the 22-G needle. Compared with 22-G EBUS-TBNA alone, sampling with both the 22- and 19-G EBUS needles resulted in an increase in diagnostic yield from 92% to 99% (P=0.045) and a number needed to sample of 13 patients to provide one additional diagnosis. There were no significant complications. In select cases where additional tissue may be needed, sampling with a 19-G EBUS needle following standard aspiration with a 22-G needle results in an increase in diagnostic yield.

  8. C57 mice increase wheel-running behavior following stress: preliminary findings.

    PubMed

    Sibold, Jeremy S; Hammack, Sayamwong E; Falls, William A

    2011-10-01

    Exercise has been shown to reduce anxiety in both humans and animals. To date, there are few, if any studies that examine the effect of stress on self-selected exercise using an animal model. This study examined the effect of acute stress on wheel-running distance in mice. Forty 8-week-old, male C57BL/6J mice were randomly assigned to one of three groups: no stress + wheel-running experience, stress + wheel-running experience, or stress with no wheel-running experience. Stressed mice were exposed to foot shock in a brightly lit environment. Following treatment, wheel-running distances were observed for three hours. Stress significantly increased voluntary wheel-running in mice with wheel-running experience as compared to nonstressed controls and stressed mice with no wheel-running experience. These results suggest that mice familiar with wheel-running may self-select this exercise as a modality for the mitigation of accumulated anxiety.

  9. Environmental stress increases variability in the expression of dental cusps.

    PubMed

    Riga, Alessandro; Belcastro, Maria Giovanna; Moggi-Cecchi, Jacopo

    2014-03-01

    Teeth are an important model for developmental studies but, despite an extensive literature on the genetics of dental development, little is known about the environmental influences on dental morphology. Here we test whether and to what extent the environment plays a role in producing morphological variation in human teeth. We selected a sample of modern human skulls and used dental enamel hypoplasia as an environmental stress marker to identify two groups with different stress levels, referred to as SG ("stressed" group) and NSG ("nonstressed" group). We collected data on the occurrence and the relative development of 15 morphological traits on upper molars using a standard methodology (ASU-DAS system) and then we compared the frequencies of the traits in the two groups. Overall, the results suggest that (a) stressors like malnutrition and/or systemic diseases have a significant effect on upper molar morphology; (b) stress generates a developmental response which increases the morphological variability of the SG; and (c) the increase in variability is directional, since individuals belonging to the SG have more developed and extra cusps. These results are consistent with the expectations of the current model of dental development. Copyright © 2013 Wiley Periodicals, Inc.

  10. Whole body heat stress increases motor cortical excitability and skill acquisition in humans

    PubMed Central

    Littmann, Andrew E.; Shields, Richard K.

    2015-01-01

    Objective Vigorous systemic exercise stimulates a cascade of molecular and cellular processes that enhance central nervous system (CNS) plasticity and performance. The influence of heat stress on CNS performance and learning is novel. We designed two experiments to determine whether passive heat stress 1) facilitated motor cortex excitability and 2) improved motor task acquisition compared to no heat stress. Methods Motor evoked potentials (MEPs) from the first dorsal interosseus (FDI) were collected before and after 30 minutes of heat stress at 73° C. A second cohort of subjects performed a motor learning task using the FDI either following heat or the no heat condition. Results Heat stress increased heart rate to 65% of age-predicted maximum. After heat, mean resting MEP amplitude increased 48% (P < 0.05). MEP stimulus-response amplitudes did not differ according to stimulus intensity. In the second experiment, heat stress caused a significant decrease in absolute and variable error (p < 0.05) during a novel movement task using the FDI. Conclusions Passive environmental heat stress 1) increases motor cortical excitability, and 2) enhances performance in a motor skill acquisition task. Significance Controlled heat stress may prime the CNS to enhance motor skill acquisition during rehabilitation. PMID:26616546

  11. Whole body heat stress increases motor cortical excitability and skill acquisition in humans.

    PubMed

    Littmann, Andrew E; Shields, Richard K

    2016-02-01

    Vigorous systemic exercise stimulates a cascade of molecular and cellular processes that enhance central nervous system (CNS) plasticity and performance. The influence of heat stress on CNS performance and learning is novel. We designed two experiments to determine whether passive heat stress (1) facilitated motor cortex excitability and (2) improved motor task acquisition compared to no heat stress. Motor evoked potentials (MEPs) from the first dorsal interosseus (FDI) were collected before and after 30 min of heat stress at 73 °C. A second cohort of subjects performed a motor learning task using the FDI either following heat or the no heat condition. Heat stress increased heart rate to 65% of age-predicted maximum. After heat, mean resting MEP amplitude increased 48% (p<0.05). MEP stimulus-response amplitudes did not differ according to stimulus intensity. In the second experiment, heat stress caused a significant decrease in absolute and variable error (p<0.05) during a novel movement task using the FDI. Passive environmental heat stress (1) increases motor cortical excitability, and (2) enhances performance in a motor skill acquisition task. Controlled heat stress may prime the CNS to enhance motor skill acquisition during rehabilitation. Copyright © 2015 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.

  12. Managing Southeastern US Forests for Increased Water Yield

    NASA Astrophysics Data System (ADS)

    Acharya, S.; Kaplan, D. A.; Mclaughlin, D. L.; Cohen, M. J.

    2017-12-01

    Forested lands influence watershed hydrology by affecting water quantity and quality in surface and groundwater systems, making them potentially effective tools for regional water resource planning. In this study, we quantified water use and water yield by pine forests under varying silvicultural management (e.g., high density plantation, thinning, and prescribed burning). Daily forest water use (evapotranspiration, ET) was estimated using continuously monitored soil-moisture in the root-zone at six sites across Florida (USA), each with six plots ranging in forest leaf-area index (LAI). Plots included stands with different rotational ages (from clear-cut to mature pine plantations) and those restored to more historical conditions. Estimated ET relative to potential ET (PET) was strongly associated with LAI, root-zone soil-moisture status, and site hydroclimate; these factors explained 85% of the variation in the ET:PET ratio. Annual water yield (Yw) calculated from these ET estimates and a simple water balance differed significantly among sites and plots (ranging from -0.12 cm/yr to > 100 cm/yr), demonstrating substantive influence of management regimes. LAI strongly influenced Yw in all sites, and a general linear model with forest attributes (LAI and groundcover), hydroclimate, and site characteristics explained >90% of variation in observed Yw. These results can be used to predict water yield changes under different management and climate scenarios and may be useful in the development of payment for ecosystem services approaches that identify water as an important product of forest best management practices.

  13. Increased reactivity and monoamine dysregulation following stress in triploid Atlantic salmon (Salmo salar).

    PubMed

    Fraser, Thomas William Kenneth; Vindas, Marco Antonio; Fjelldal, Per Gunnar; Winberg, Svante; Thörnqvist, Per-Ove; Øverli, Øyvind; Skjæraasen, Jon-Egil; Hansen, Tom Jonny; Mayer, Ian

    2015-07-01

    Artificial triploid salmonids are sterile and therefore commercially bred to prevent genetic interactions between wild and domestic fish strains. The full biological effects of having an extra chromosome set are largely unknown, but triploids are considered to be more sensitive to sub-optimal environmental conditions and to be stressed by the presence of diploid conspecifics. Brain serotonergic and dopaminergic activity are known to regulate the stress response in vertebrates, but monoamine systems in diploid and triploid fish have yet to be compared. Here we study monoamine neurochemistry in the telencephalon and brain stem of juvenile diploid and triploid Atlantic salmon (Salmo salar) in response to stress (unstressed vs stressed individuals) and holding (separate- vs mixed-ploidy) conditions. Both diploids and triploids showed an increase in serotonergic activity following stress, but the increase was significantly greater in the telencephalon of triploids compared to diploids. Furthermore, while telencephalic dopaminergic activity was significantly increased in diploids following stress, there was no response in triploids. Holding conditions had a significant effect on dopaminergic activity in the brain stem of diploids only, with lower values in mixed- compared to separate-ploidy conditions. These results suggest artificially produced triploids experience increased reactivity and monoaminergic dysregulation following stress that may impede their welfare and performance. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Age-dependent increase in oxidative stress in gastrocnemius muscle with unloading

    PubMed Central

    Siu, Parco M.; Pistilli, Emidio E.; Alway, Stephen E.

    2008-01-01

    Oxidative stress increases during unloading in muscle from young adult rats. The present study examined the markers of oxidative stress and antioxidant enzyme gene and protein expressions in medial gastrocnemius muscles of aged and young adult (30 and 6 mo of age) Fischer 344 × Brown Norway rats after 14 days of hindlimb suspension. Medial gastrocnemius muscle weight was decreased by ∼30% in young adult and aged rats following suspension. When muscle weight was normalized to animal body weight, it was reduced by 12% and 22% in young adult and aged rats, respectively, after suspension. Comparisons between young adult and aged control animals demonstrated a 25% and 51% decline in muscle mass when expressed as absolute muscle weight and muscle weight normalized to the animal body weight, respectively. H2O2 content was elevated by 43% while Mn superoxide dismutase (MnSOD) protein content was reduced by 28% in suspended muscles compared with control muscles exclusively in the aged animals. Suspended muscles had greater content of malondialdehyde (MDA)/4-hydroxyalkenals (4-HAE) (29% and 58% increase in young adult and aged rats, respectively), nitrotyrosine (76% and 65% increase in young adult and aged rats, respectively), and catalase activity (69% and 43% increase in young adult and aged rats, respectively) relative to control muscles. Changes in oxidative stress markers MDA/4-HAE, H2O2, and MnSOD protein contents in response to hindlimb unloading occurred in an age-dependent manner. These findings are consistent with the hypotheses that oxidative stress has a role in mediating disuse-induced and sarcopenia-associated muscle losses. Our data suggest that aging may predispose skeletal muscle to increased levels of oxidative stress both at rest and during unloading. PMID:18801960

  15. Stress Increases Peripheral Axon Growth and Regeneration through Glucocorticoid Receptor-Dependent Transcriptional Programs

    PubMed Central

    Alexander, Jessica K.; Madalena, Kathryn M.; Motti, Dario; Quach, Tam; Zha, Alicia; Webster Marketon, Jeanette

    2017-01-01

    Abstract Stress and glucocorticoid (GC) release are common behavioral and hormonal responses to injury or disease. In the brain, stress/GCs can alter neuron structure and function leading to cognitive impairment. Stress and GCs also exacerbate pain, but whether a corresponding change occurs in structural plasticity of sensory neurons is unknown. Here, we show that in female mice (Mus musculus) basal GC receptor (Nr3c1, also known as GR) expression in dorsal root ganglion (DRG) sensory neurons is 15-fold higher than in neurons in canonical stress-responsive brain regions (M. musculus). In response to stress or GCs, adult DRG neurite growth increases through mechanisms involving GR-dependent gene transcription. In vivo, prior exposure to an acute systemic stress increases peripheral nerve regeneration. These data have broad clinical implications and highlight the importance of stress and GCs as novel behavioral and circulating modifiers of neuronal plasticity. PMID:28828403

  16. Optimizing rice yields while minimizing yield-scaled global warming potential.

    PubMed

    Pittelkow, Cameron M; Adviento-Borbe, Maria A; van Kessel, Chris; Hill, James E; Linquist, Bruce A

    2014-05-01

    To meet growing global food demand with limited land and reduced environmental impact, agricultural greenhouse gas (GHG) emissions are increasingly evaluated with respect to crop productivity, i.e., on a yield-scaled as opposed to area basis. Here, we compiled available field data on CH4 and N2 O emissions from rice production systems to test the hypothesis that in response to fertilizer nitrogen (N) addition, yield-scaled global warming potential (GWP) will be minimized at N rates that maximize yields. Within each study, yield N surplus was calculated to estimate deficit or excess N application rates with respect to the optimal N rate (defined as the N rate at which maximum yield was achieved). Relationships between yield N surplus and GHG emissions were assessed using linear and nonlinear mixed-effects models. Results indicate that yields increased in response to increasing N surplus when moving from deficit to optimal N rates. At N rates contributing to a yield N surplus, N2 O and yield-scaled N2 O emissions increased exponentially. In contrast, CH4 emissions were not impacted by N inputs. Accordingly, yield-scaled CH4 emissions decreased with N addition. Overall, yield-scaled GWP was minimized at optimal N rates, decreasing by 21% compared to treatments without N addition. These results are unique compared to aerobic cropping systems in which N2 O emissions are the primary contributor to GWP, meaning yield-scaled GWP may not necessarily decrease for aerobic crops when yields are optimized by N fertilizer addition. Balancing gains in agricultural productivity with climate change concerns, this work supports the concept that high rice yields can be achieved with minimal yield-scaled GWP through optimal N application rates. Moreover, additional improvements in N use efficiency may further reduce yield-scaled GWP, thereby strengthening the economic and environmental sustainability of rice systems. © 2013 John Wiley & Sons Ltd.

  17. Respiratory sinus arrhythmia during worry forecasts stress-related increases in psychological distress.

    PubMed

    Gouin, Jean-Philippe; Deschênes, Sonya S; Dugas, Michel J

    2014-09-01

    Respiratory sinus arrhythmia (RSA) has been conceptualized as an index of emotion regulation abilities. Although resting RSA has been associated with both concurrent and prospective affective responses to stress, the impact of RSA reactivity on emotional responses to stress is inconsistent across studies. The type of emotional stimuli used to elicit these phasic RSA responses may influence the adaptive value of RSA reactivity. We propose that RSA reactivity to a personally relevant worry-based stressor might forecast future affective responses to stress. To evaluate whether resting RSA and RSA reactivity to worry inductions predict stress-related increases in psychological distress, an academic stress model was used to prospectively examine changes in psychological distress from the well-defined low- and high-stress periods. During the low-stress period, 76 participants completed self-report mood measures and had their RSA assessed during a resting baseline, free worry period and worry catastrophizing interview. Participants completed another mood assessment during the high-stress period. Results indicated that baseline psychological distress predicted larger decreases in RSA during the worry inductions. Lower resting RSA and greater RSA suppression to the worry inductions at baseline prospectively predicted larger increases in psychological distress from the low- to high-stress period, even after accounting for the impact of baseline distress on RSA. These results provide further evidence that RSA may represent a unique index of emotion regulation abilities in times of stress.

  18. Bisphenol A increases BeWo trophoblast survival in stress-induced paradigms through regulation of oxidative stress and apoptosis.

    PubMed

    Ponniah, Muralitharan; Billett, E Ellen; De Girolamo, Luigi A

    2015-09-21

    Bisphenol A (BPA) is ubiquitous in the environment and is reported to be present at high concentrations in placental tissue, where its presence raises concerns over its potential to disrupt placental function. This report investigates how BPA interferes with the survival of human choriocarcinoma BeWo cells (a model of placental trophoblasts) under stress-induced paradigms reminiscent of pathways activated in placental development. These include conditions that promote oxidative stress (glutathione depletion) and apoptosis (serum withdrawal) or mimic hypoxia (HIF-1α accumulation via dimethyloxalylglycine treatment). Treatment of BeWo cells with BPA during stress-induced paradigms led to a consistent and significant increase in cell viability, with a concomitant increase in glutathione levels and a reduction in apoptosis. Assessment of the antioxidant capacity of BPA revealed its ability to quench reactive oxygen species and reduce the levels generated during glutathione and serum depletion. BPA was also able to reduce the activation of the antioxidant response element (ARE) through mediation of its activators, nuclear factor erythroid related factor family members (Nrf's). Indeed, the expression and nuclear translocation of Nrf2 (an important ARE activator) were impaired by BPA, while Nrf1 and Nrf3 expression levels were increased. Furthermore, BPA increased the levels of the anti-apoptotic proteins (Bcl-2 and Hsp70) and decreased HIF-1α levels during stress-induced conditions. Together, these results indicate that BPA inhibits trophoblast cell death under conditions of cellular stress. This could have implications on placental trophoblasts during development.

  19. Increased global transcription activity as a mechanism of replication stress in cancer

    PubMed Central

    Kotsantis, Panagiotis; Silva, Lara Marques; Irmscher, Sarah; Jones, Rebecca M.; Folkes, Lisa; Gromak, Natalia; Petermann, Eva

    2016-01-01

    Cancer is a disease associated with genomic instability that often results from oncogene activation. This in turn leads to hyperproliferation and replication stress. However, the molecular mechanisms that underlie oncogene-induced replication stress are still poorly understood. Oncogenes such as HRASV12 promote proliferation by upregulating general transcription factors to stimulate RNA synthesis. Here we investigate whether this increase in transcription underlies oncogene-induced replication stress. We show that in cells overexpressing HRASV12, elevated expression of the general transcription factor TATA-box binding protein (TBP) leads to increased RNA synthesis, which together with R-loop accumulation results in replication fork slowing and DNA damage. Furthermore, overexpression of TBP alone causes the hallmarks of oncogene-induced replication stress, including replication fork slowing, DNA damage and senescence. Consequently, we reveal that increased transcription can be a mechanism of oncogene-induced DNA damage, providing a molecular link between upregulation of the transcription machinery and genomic instability in cancer. PMID:27725641

  20. Increased global transcription activity as a mechanism of replication stress in cancer.

    PubMed

    Kotsantis, Panagiotis; Silva, Lara Marques; Irmscher, Sarah; Jones, Rebecca M; Folkes, Lisa; Gromak, Natalia; Petermann, Eva

    2016-10-11

    Cancer is a disease associated with genomic instability that often results from oncogene activation. This in turn leads to hyperproliferation and replication stress. However, the molecular mechanisms that underlie oncogene-induced replication stress are still poorly understood. Oncogenes such as HRAS V12 promote proliferation by upregulating general transcription factors to stimulate RNA synthesis. Here we investigate whether this increase in transcription underlies oncogene-induced replication stress. We show that in cells overexpressing HRAS V12 , elevated expression of the general transcription factor TATA-box binding protein (TBP) leads to increased RNA synthesis, which together with R-loop accumulation results in replication fork slowing and DNA damage. Furthermore, overexpression of TBP alone causes the hallmarks of oncogene-induced replication stress, including replication fork slowing, DNA damage and senescence. Consequently, we reveal that increased transcription can be a mechanism of oncogene-induced DNA damage, providing a molecular link between upregulation of the transcription machinery and genomic instability in cancer.

  1. Phosphoketolase overexpression increases biomass and lipid yield from methane in an obligate methanotrophic biocatalyst

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Henard, Calvin A.; Smith, Holly K.; Guarnieri, Michael T.

    Microbial conversion of methane to high-value bio-based chemicals and materials offers a path to mitigate GHG emissions and valorize this abundant-yet -underutilized carbon source. In addition to fermentation optimization strategies, rational methanotrophic bacterial strain engineering offers a means to reach industrially relevant titers, carbon yields, and productivities of target products. The phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. Importantly, this pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO 2 lost through pyruvate decarboxylation in themore » Embden-Meyerhof-Parnas pathway. Thus, the phosphoketolase pathway can be leveraged for carbon efficient biocatalysis to acetyl-CoA-derived intermediates and products. Here, we show that the industrially promising methane biocatalyst, Methylomicrobium buryatense, encodes two phosphoketolase isoforms that are expressed in methanol- and methane-grown cells. Overexpression of the PktB isoform led to a 2-fold increase in intracellular acetyl-CoA concentration, and a 2.6-fold yield enhancement from methane to microbial biomass and lipids compared to wild-type, increasing the potential for methanotroph lipid-based fuel production. Off-gas analysis and metabolite profiling indicated that global metabolic rearrangements, including significant increases in post-translational protein acetylation and gene expression of the tetrahydromethanopterin-linked pathway, along with decreases in several excreted products, coincided with the superior biomass and lipid yield observed in the engineered strain. Further, these data suggest that phosphoketolase may play a key regulatory role in methanotrophic bacterial metabolism. As a result, given that acetyl-CoA is a key intermediate in several biosynthetic pathways, phosphoketolase

  2. Phosphoketolase overexpression increases biomass and lipid yield from methane in an obligate methanotrophic biocatalyst.

    PubMed

    Henard, Calvin A; Smith, Holly K; Guarnieri, Michael T

    2017-05-01

    Microbial conversion of methane to high-value bio-based fuels, chemicals, and materials offers a path to mitigate GHG emissions and valorize this abundant-yet -underutilized carbon source. In addition to fermentation optimization strategies, rational methanotrophic bacterial strain engineering offers a means to reach industrially relevant titers, carbon yields, and productivities of target products. The phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. Importantly, this pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO 2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway. Thus, the phosphoketolase pathway can be leveraged for carbon efficient biocatalysis to acetyl-CoA-derived intermediates and products. Here, we show that the industrially promising methane biocatalyst, Methylomicrobium buryatense, encodes two phosphoketolase isoforms that are expressed in methanol- and methane-grown cells. Overexpression of the PktB isoform led to a 2-fold increase in intracellular acetyl-CoA concentration, and a 2.6-fold yield enhancement from methane to microbial biomass and lipids compared to wild-type, increasing the potential for methanotroph lipid-based fuel production. Off-gas analysis and metabolite profiling indicated that global metabolic rearrangements, including significant increases in post-translational protein acetylation and gene expression of the tetrahydromethanopterin-linked pathway, along with decreases in several excreted products, coincided with the superior biomass and lipid yield observed in the engineered strain. Further, these data suggest that phosphoketolase may play a key regulatory role in methanotrophic bacterial metabolism. Given that acetyl-CoA is a key intermediate in several biosynthetic pathways, phosphoketolase overexpression offers a

  3. Phosphoketolase overexpression increases biomass and lipid yield from methane in an obligate methanotrophic biocatalyst

    DOE PAGES

    Henard, Calvin A.; Smith, Holly K.; Guarnieri, Michael T.

    2017-04-02

    Microbial conversion of methane to high-value bio-based chemicals and materials offers a path to mitigate GHG emissions and valorize this abundant-yet -underutilized carbon source. In addition to fermentation optimization strategies, rational methanotrophic bacterial strain engineering offers a means to reach industrially relevant titers, carbon yields, and productivities of target products. The phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. Importantly, this pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO 2 lost through pyruvate decarboxylation in themore » Embden-Meyerhof-Parnas pathway. Thus, the phosphoketolase pathway can be leveraged for carbon efficient biocatalysis to acetyl-CoA-derived intermediates and products. Here, we show that the industrially promising methane biocatalyst, Methylomicrobium buryatense, encodes two phosphoketolase isoforms that are expressed in methanol- and methane-grown cells. Overexpression of the PktB isoform led to a 2-fold increase in intracellular acetyl-CoA concentration, and a 2.6-fold yield enhancement from methane to microbial biomass and lipids compared to wild-type, increasing the potential for methanotroph lipid-based fuel production. Off-gas analysis and metabolite profiling indicated that global metabolic rearrangements, including significant increases in post-translational protein acetylation and gene expression of the tetrahydromethanopterin-linked pathway, along with decreases in several excreted products, coincided with the superior biomass and lipid yield observed in the engineered strain. Further, these data suggest that phosphoketolase may play a key regulatory role in methanotrophic bacterial metabolism. As a result, given that acetyl-CoA is a key intermediate in several biosynthetic pathways, phosphoketolase

  4. Yield gaps and yield relationships in US soybean production systems

    USDA-ARS?s Scientific Manuscript database

    The magnitude of yield gaps (YG) (potential yield – farmer yield) provides some indication of the prospects for increasing crop yield to meet the food demands of future populations. Quantile regression analysis was applied to county soybean [Glycine max (L.) Merrill] yields (1971 – 2011) from Kentuc...

  5. Stress-induced alterations in estradiol sensitivity increase risk for obesity in women.

    PubMed

    Michopoulos, Vasiliki

    2016-11-01

    The prevalence of obesity in the United States continues to rise, increasing individual vulnerability to an array of adverse health outcomes. One factor that has been implicated causally in the increased accumulation of fat and excess food intake is the activity of the limbic-hypothalamic-pituitary-adrenal (LHPA) axis in the face of relentless stressor exposure. However, translational and clinical research continues to understudy the effects sex and gonadal hormones and LHPA axis dysfunction in the etiology of obesity even though women continue to be at greater risk than men for stress-induced disorders, including depression, emotional feeding and obesity. The current review will emphasize the need for sex-specific evaluation of the relationship between stress exposure and LHPA axis activity on individual risk for obesity by summarizing data generated by animal models currently being leveraged to determine the etiology of stress-induced alterations in feeding behavior and metabolism. There exists a clear lack of translational models that have been used to study female-specific risk. One translational model of psychosocial stress exposure that has proven fruitful in elucidating potential mechanisms by which females are at increased risk for stress-induced adverse health outcomes is that of social subordination in socially housed female macaque monkeys. Data from subordinate female monkeys suggest that increased risk for emotional eating and the development of obesity in females may be due to LHPA axis-induced changes in the behavioral and physiological sensitivity of estradiol. The lack in understanding of the mechanisms underlying these alterations necessitate the need to account for the effects of sex and gonadal hormones in the rationale, design, implementation, analysis and interpretation of results in our studies of stress axis function in obesity. Doing so may lead to the identification of novel therapeutic targets with which to combat stress-induced obesity

  6. Stress-Induced Alterations in Estradiol Sensitivity Increase Risk for Obesity in Women

    PubMed Central

    Michopoulos, Vasiliki

    2016-01-01

    The prevalence of obesity in the United States continues to rise, increasing individual vulnerability to an array of adverse health outcomes. One factor that has been implicated causally in the increased accumulation of fat and excess food intake is the activity of the limbic-hypothalamic-pituitary-adrenal (LHPA) axis in the face of relentless stressor exposure. However, translational and clinical research continues to understudy the effects sex and gonadal hormones and LHPA axis dysfunction in the etiology of obesity even though women continue to be at greater risk than men for stress-induced disorders, including depression, emotional feeding and obesity. The current review will emphasize the need for sex-specific evaluation of the relationship between stress exposure and LHPA axis activity on individual risk for obesity by summarizing data generated by animal models currently being leveraged to determine the etiology of stress-induced alterations in feeding behavior and metabolism. There exists a clear lack of translational models that have been used to study female-specific risk. One translational model of psychosocial stress exposure that has proven fruitful in elucidating potential mechanisms by which females are at increased risk for stress-induced adverse health outcomes is that of social subordination in socially housed female macaque monkeys. Data from subordinate female monkeys suggest that increased risk for emotional eating and the development of obesity in females may be due to LHPA axis-induced changes in the behavioral and physiological sensitivity of estradiol. The lack in understanding of the mechanisms underlying these alterations necessitate the need to account for the effects of sex and gonadal hormones in the rationale, design, implementation, analysis and interpretation of results in our studies of stress axis function in obesity. Doing so may lead to the identification of novel therapeutic targets with which to combat stress-induced obesity

  7. Mechanical design of mussel byssus: material yield enhances attachment strength

    PubMed

    Bell; Gosline

    1996-01-01

    The competitive dominance of mussels in the wave-swept rocky intertidal zone is in part due to their ability to maintain a secure attachment. Mussels are tethered to the substratum by a byssus composed of numerous extracellular, collagenous threads secreted by the foot. Each byssal thread has three serially arranged parts: a corrugated proximal region, a smooth distal region and an adhesive plaque. This study examines the material and structural properties of the byssal threads of three mussel species: Mytilus californianus, M. trossulus, and M. galloprovincialis. Tensile tests in general reveal similar material properties among species: the proximal region has a lower initial modulus, a lower ultimate stress and a higher ultimate strain than the distal region. The distal region also yields at a stress well below its ultimate value. In whole thread tests, the proximal region and adhesive plaque are common sites of structural failure and are closely matched in strength, while the distal region appears to be excessively strong. We propose that the high strength of the distal region is the byproduct of a material designed to yield and extend before structural failure occurs. Experimental and theoretical evidence is presented suggesting that thread yield and extensibility provide two important mechanisms for increasing the overall attachment strength of the mussel: (1) the reorientation of threads towards the direction of applied load, and (2) the 'recruitment' of more threads into tension and the consequent distribution of applied load over a larger cross-sectional area, thereby reducing the stress on each thread. This distal region yield behavior is most striking for M. californianus and may be a key to its success in extreme wave-swept environments.

  8. Soybean grown under elevated CO2 benefits more under low temperature than high temperature stress: Varying response of photosynthetic limitations, leaf metabolites, growth, and seed yield.

    PubMed

    Xu, Guangli; Singh, Shardendu K; Reddy, Vangimalla R; Barnaby, Jinyoung Y; Sicher, Richard C; Li, Tian

    2016-10-20

    To evaluate the combined effect of temperature and CO 2 on photosynthetic processes, leaf metabolites and growth, soybean was grown under a controlled environment at low (22/18°C, LT), optimum (28/24°C, OT) and high (36/32°C HT) temperatures under ambient (400μmolmol -1 ; aCO 2 ) or elevated (800μmolmol -1 ; eCO 2 ) CO 2 concentrations during the reproductive stage. In general, the rate of photosynthesis (A), stomatal (g s ) and mesophyll (g m ) conductance, quantum yield of photosystem II, rates of maximum carboxylation (V Cmax ), and electron transport (J) increased with temperature across CO 2 levels. However, compared with OT, the percentage increases in these parameters at HT were lower than the observed decline at LT. The photosynthetic limitation at LT and OT was primarily caused by photo-biochemical processes (49-58%, L b ) followed by stomatal (27-32%, L s ) and mesophyll (15-19%, L m ) limitations. However, at HT, it was primarily caused by L s (41%) followed by L b (33%) and L m (26%). The dominance of L b at LT and OT was associated with the accumulation of non-structural carbohydrates (e.g., starch) and several organic acids, whereas this accumulation did not occur at HT, indicating increased metabolic activities. Compared with OT, biomass and seed yield declined more at HT than at LT. The eCO 2 treatment compensated for the temperature-stress effects on biomass but only partially compensated for the effects on seed yield, especially at HT. Photosynthetic downregulation at eCO 2 was possibly due to the accumulation of non-structural carbohydrates and the decrease in g s and A std (standard A measured at 400μmolmol -1 sub-stomatal CO 2 concentration), as well as the lack of CO 2 effect on g m , V Cmax , and J, and photosynthetic limitation. Thus, the photosynthetic limitation was temperature-dependent and was primarily influenced by the alteration in photo-biochemical processes and metabolic activities. Despite the inconsistent response of

  9. Effect of dietary probiotic and high stocking density on the performance, carcass yield, gut microflora, and stress indicators of broilers.

    PubMed

    Cengiz, Özcan; Köksal, Bekir H; Tatlı, Onur; Sevim, Ömer; Ahsan, Umair; Üner, Aykut G; Ulutaş, Pınar A; Beyaz, Devrim; Büyükyörük, Sadık; Yakan, Akın; Önol, Ahmet G

    2015-10-01

    A study was carried out to evaluate the effect of dietary probiotic supplementation and stocking density on the performance, relative carcass yield, gut microflora, and stress markers of broilers. One-day-old Ross 308 male broiler chickens (n = 480) were allocated to 4 experimental groups for 42 d. Each treatment had 8 replicates of 15 chicks each. Two groups were subjected to a high stocking density (HSD) of 20 birds/m² and the other 2 groups were kept at low stocking density (LSD) of 10 birds/m². A basal diet supplemented with probiotic 1 and 0.5 g/kg of diet (in starter and finisher diets, respectively) was fed to 2 treatments, one with HSD and the other with LSD, thereby making a 2 × 2 factorial arrangement. There was no interaction between stocking density (LSD and HSD) and dietary probiotic (supplemented and unsupplemented) for all the variables. Feed intake and weight gain were significantly low and feed conversion ratio was poor in broilers at HSD. Dietary probiotic significantly enhanced the feed intake and weight gain in starter phase only. Dietary probiotic supplementation had no effect (P > 0.05) on total aerobs, Salmonella sp., and Lactobacilli populations in the intestines of broilers. However, HSD reduced the Lactobacilli population only (P < 0.05). Relative breast yields were significantly higher in broilers reared at LSD than HSD. Thigh meat yield was higher in broilers in HSD group compared to LSD. Dietary probiotic did not affect the relative carcass yield and weight of lymphoid organs. Serum malondialdehyde, corticosterone, nitric oxide, and plasma heterophil:lymphocyte ratio were not affected either by stocking density or dietary probiotic supplementation. In conclusion, HSD negatively affected the performance and intestinal Lactobacilli population of broilers only, whereas probiotic supplementation enhanced the performance of broilers during the starter phase only. Total aerobes, Salmonella, Lactobacilli carcass yield, and stress indicators

  10. Climate risks on potato yield in Europe

    NASA Astrophysics Data System (ADS)

    Sun, Xun; Lall, Upmanu

    2016-04-01

    The yield of potatoes is affected by water and temperature during the growing season. We study the impact of a suite of climate variables on potato yield at country level. More than ten climate variables related to the growth of potato are considered, including the seasonal rainfall and temperature, but also extreme conditions at different averaging periods from daily to monthly. A Bayesian hierarchical model is developed to jointly consider the risk of heat stress, cold stress, wet and drought. Future climate risks are investigated through the projection of future climate data. This study contributes to assess the risks of present and future climate risks on potatoes yield, especially the risks of extreme events, which could be used to guide better sourcing strategy and ensure food security in the future.

  11. [Emotional stress-induced Shanghuo syndrome increases disease susceptibility].

    PubMed

    Zhu, Si-Rui; Luo, Xiang; Li, Yi-Fang; Hiroshi, Kurihara; He, Rong-Rong

    2018-04-01

    Shanghuo(excessive internal heat) is a special organic state based on the concept of traditional Chinese medicine(TCM), commonly known as the abnormal heating syndrome of body in folks. With the acceleration of modern life rhythm and the increase of the social competition pressure, emotional stress has become an important cause for the spread of Shanghuo symptoms. What's more, Shanghuo can impact the body physiological functions to cause the onset, recurrence and progression of common diseases, harming the health of the body. According to the long-term research findings, the author found that Shanghuo referred to the imbalance of multiple physiological functions, such as nerve, immunity and metabolism, caused by emotional stress. "Shanghuo" is not a disease itself, but it can increase the susceptibility to a variety of diseases. This study reviewed the traditional medicine theory and the modern medical studies, and explored the relevance and correlation mechanisms between the Shanghuo symptoms and disease susceptibility, so as to provide a reference to improve the state of sub-health and prevent or treat modern diseases. Copyright© by the Chinese Pharmaceutical Association.

  12. Dairy cattle in a temperate climate: the effects of weather on milk yield and composition depend on management.

    PubMed

    Hill, D L; Wall, E

    2015-01-01

    A better understanding of how livestock respond to weather is essential to enable farming to adapt to a changing climate. Climate change is mainly expected to impact dairy cattle through heat stress and an increase in the frequency of extreme weather events. We investigated the effects of weather on milk yield and composition (fat and protein content) in an experimental dairy herd in Scotland over 21 years. Holstein Friesian cows were either housed indoors in winter and grazed over the summer or were continuously housed. Milk yield was measured daily, resulting in 762 786 test day records from 1369 individuals, and fat and protein percentage were sampled once a week, giving 89 331 records from 1220 cows/trait. The relative influence of 11 weather elements, measured from local outdoor weather stations, and two indices of temperature and humidity (THI), indicators of heat stress, were compared using separate maximum likelihood models for each element or index. Models containing a direct measure of temperature (dry bulb, wet bulb, grass or soil temperature) or a THI provided the best fits to milk yield and fat data; wind speed and the number of hours of sunshine were most important in explaining protein content. Weather elements summarised across a week's timescale from the test day usually explained milk yield and fat content better than shorter-scale (3 day, test day, test day -1) metrics. Then, examining a subset of key weather variables using restricted maximum likelihood, we found that THI, wind speed and the number of hours of sunshine influenced milk yield and composition. The shape and magnitude of these effects depended on whether animals were inside or outside on the test day. The milk yield of cows outdoors was lower at the extremes of THI than at average values, and the highest yields were obtained when THI, recorded at 0900 h, was 55 units. Cows indoors decreased milk yield as THI increased. Fat content was lower at higher THIs than at intermediate THIs

  13. Elimination of metabolic pathways to all traditional fermentation products increases ethanol yields in Clostridium thermocellum

    DOE PAGES

    Papanek, Beth A.; Biswas, Ranjita; Rydzak, Thomas; ...

    2015-09-12

    Clostridium thermocellum has the natural ability to convert cellulose to ethanol, making it a promising candidate for consolidated bioprocessing (CBP) of cellulosic biomass to biofuels. To further improve its CBP capabilities, we study a mutant strain of C. thermocellum that was constructed (strain AG553; C. thermocellum Δhpt ΔhydG Δldh Δpfl Δpta-ack) to increase flux to ethanol by removing side product formation. Strain AG553 showed a two- to threefold increase in ethanol yield relative to the wild type on all substrates tested. On defined medium, strain AG553 exceeded 70% of theoretical ethanol yield on lower loadings of the model crystalline cellulosemore » Avicel, effectively eliminating formate, acetate, and lactate production and reducing H 2 production by fivefold. On 5 g/L Avicel, strain AG553 reached an ethanol yield of 63.5% of the theoretical maximum compared with 19.9% by the wild type, and it showed similar yields on pretreated switchgrass and poplar. The elimination of organic acid production suggested that the strain might be capable of growth under higher substrate loadings in the absence of pH control. Final ethanol titer peaked at 73.4 mM in mutant AG553 on 20 g/L Avicel, at which point the pH decreased to a level that does not allow growth of C. thermocellum, likely due to CO 2 accumulation. In comparison, the maximum titer of wild type C. thermocellum was 14.1 mM ethanol on 10 g/L Avicel. In conclusion, with the elimination of the metabolic pathways to all traditional fermentation products other than ethanol, AG553 is the best ethanol-yielding CBP strain to date and will serve as a platform strain for further metabolic engineering for the bioconversion of lignocellulosic biomass.« less

  14. Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress.

    PubMed

    Oksala, Niku K J; Ekmekçi, F Güler; Ozsoy, Ergi; Kirankaya, Serife; Kokkola, Tarja; Emecen, Güzin; Lappalainen, Jani; Kaarniranta, Kai; Atalay, Mustafa

    2014-01-01

    Heat shock proteins (HSPs), originally identified as heat-inducible gene products, are a family of highly conserved proteins that respond to a wide variety of stress including oxidative stress. Although both acute and chronic oxidative stress have been well demonstrated to induce HSP responses, little evidence is available whether increased HSP levels provide enhanced protection against oxidative stress under elevated yet sublethal temperatures. We studied relationships between oxidative stress and HSPs in a physiological model by using Garra rufa (doctor fish), a fish species naturally acclimatized to different thermal conditions. We compared fish naturally living in a hot spring with relatively high water temperature (34.4±0.6°C) to those living in normal river water temperature (25.4±4.7°C), and found that levels of all the studied HSPs (HSP70, HSP60, HSP90, HSC70 and GRP75) were higher in fish living in elevated water temperature compared with normal river water temperature. In contrast, indicators of oxidative stress, including protein carbonyls and lipid hydroperoxides, were decreased in fish living in the elevated temperature, indicating that HSP levels are inversely associated with oxidative stress. The present results provide evidence that physiologically increased HSP levels provide protection against oxidative stress and enhance cytoprotection. Copyright © 2014 The Authors. Published by Elsevier B.V. All rights reserved.

  15. Does repeated pleural culture increase the diagnostic yield of Mycobacterium tuberculosis from tuberculous pleural effusion in HIV-negative individuals?

    PubMed

    Ko, Yousang; Song, Jinkyung; Lee, Suh-Young; Moon, Jin-Wook; Mo, Eun-Kyung; Park, Ji Young; Kim, Joo-Hee; Park, Sunghoon; Hwang, Yong Il; Jang, Seung Hun; Jhun, Byung Woo; Sim, Yun Su; Shin, Tae Rim; Kim, Dong-Gyu; Hong, Ji Young; Lee, Chang Youl; Lee, Myung Goo; Kim, Cheol-Hong; Hyun, In Gyu; Park, Yong Bum

    2017-01-01

    Despite recent advances in methods for culturing Mycobacterium tuberculosis (MTB), the diagnostic yield of tuberculous pleural effusion (TBPE) remains unsatisfactory. However, unlike repeated sputum cultures of pulmonary tuberculosis, little is known about the role of repeated pleural cultures. We examined whether repeated pleural cultures are associated with increased MTB yield from TBPE. A multicenter, retrospective cohort study was performed from January 2012 to December 2015 in South Korea. Patients were categorized into two groups: single- or repeated-culture groups. The diagnostic yield of MTB and clinical, radiological, and pleural fluid characteristics were evaluated. Among the 329 patients with TBPE, 77 (23.4%) had repeated cultures and 252 (76.5%) had a single culture. Pleural culture was performed twice in all 77 patients in the repeated-culture group at a 1-day interval (inter-quartile range, 1.0-2.0). In the repeated-culture group, the yield of MTB from the first culture was 31.2%, which was similar to that in the single-culture group (31.2% vs. 29.8%, P = 0.887). However, the yield of MTB from the second culture (10/77, 13.0%) was more than that from the first. These results may be attributable to the insufficient immune clearance for MTB invasion into the pleural space between the first and second cultures. Over time, the yield of the second cultures decreased from 17.4% to 6.7% and then 6.3%. Finally, the overall yield of MTB in the repeated- and single-culture groups was 44.2% and 29.8% respectively (P < 0.001). The results showed that repeated pleural cultures increased MTB yield from TBPE in human immunodeficiency virus-negative individuals. Furthermore, repeated cultures may increase yield when carried out for two consecutive days.

  16. Vertical farming increases lettuce yield per unit area compared to conventional horizontal hydroponics.

    PubMed

    Touliatos, Dionysios; Dodd, Ian C; McAinsh, Martin

    2016-08-01

    Vertical farming systems (VFS) have been proposed as an engineering solution to increase productivity per unit area of cultivated land by extending crop production into the vertical dimension. To test whether this approach presents a viable alternative to horizontal crop production systems, a VFS (where plants were grown in upright cylindrical columns) was compared against a conventional horizontal hydroponic system (HHS) using lettuce ( Lactuca sativa L . cv. "Little Gem") as a model crop. Both systems had similar root zone volume and planting density. Half-strength Hoagland's solution was applied to plants grown in perlite in an indoor controlled environment room, with metal halide lamps providing artificial lighting. Light distribution (photosynthetic photon flux density, PPFD) and yield (shoot fresh weight) within each system were assessed. Although PPFD and shoot fresh weight decreased significantly in the VFS from top to base, the VFS produced more crop per unit of growing floor area when compared with the HHS. Our results clearly demonstrate that VFS presents an attractive alternative to horizontal hydroponic growth systems and suggest that further increases in yield could be achieved by incorporating artificial lighting in the VFS.

  17. The effect of stress on hydrogen uptake and desorption by A-286

    NASA Technical Reports Server (NTRS)

    Danford, Merlin D.

    1991-01-01

    The uptake and desorption of hydrogen by A-286 as a function of stress was studied using electrochemical methods. It was found that the apparent surface hydrogen concentration, the mean hydrogen concentration, and the hydrogen distribution uniformity all increased up to a stress level 50 percent of yield and decreased thereafter. The value of the hydrogen diffusion coefficient was relatively unaffected by stress while the percent of trapped hydrogen appeared to decrease with increasing stress.

  18. Prazosin Prevents Increased Anxiety Behavior That Occurs in Response to Stress During Alcohol Deprivations.

    PubMed

    Rasmussen, Dennis D; Kincaid, Carrie L; Froehlich, Janice C

    2017-01-01

    Stress-induced anxiety is a risk factor for relapse to alcohol drinking. The aim of this study was to test the hypothesis that the central nervous system (CNS)-active α 1 -adrenergic receptor antagonist, prazosin, would block the stress-induced increase in anxiety that occurs during alcohol deprivations. Selectively bred male alcohol-preferring (P) rats were given three cycles of 5 days of ad libitum voluntary alcohol drinking interrupted by 2 days of alcohol deprivation, with or without 1 h of restraint stress 4 h after the start of each of the first two alcohol deprivation cycles. Prazosin (1.0 or 1.5 mg/kg, IP) or vehicle was administered before each restraint stress. Anxiety-like behavior during alcohol deprivation following the third 5-day cycle of alcohol drinking (7 days after the most recent restraint stress ± prazosin treatment) was measured by performance in an elevated plus-maze and in social approach/avoidance testing. Rats that received constant alcohol access, or alcohol access and deprivations without stress or prazosin treatments in the first two alcohol deprivations did not exhibit augmented anxiety-like behavior during the third deprivation. In contrast, rats that had been stressed during the first two alcohol deprivations exhibited increased anxiety-like behavior (compared with control rats) in both anxiety tests during the third deprivation. Prazosin given before stresses in the first two cycles of alcohol withdrawal prevented increased anxiety-like behavior during the third alcohol deprivation. Prazosin treatment before stresses experienced during alcohol deprivations may prevent the increased anxiety during subsequent deprivation/abstinence that is a risk factor for relapse to alcohol drinking. Administration of prazosin before stresses during repetitive alcohol deprivations in male alcohol-preferring (P) rats prevents increased anxiety during a subsequent deprivation without further prazosin treatment. Prazosin treatment during repeated

  19. Prazosin Prevents Increased Anxiety Behavior That Occurs in Response to Stress During Alcohol Deprivations

    PubMed Central

    Rasmussen, Dennis D.; Kincaid, Carrie L.; Froehlich, Janice C.

    2017-01-01

    Aims Stress-induced anxiety is a risk factor for relapse to alcohol drinking. The aim of this study was to test the hypothesis that the central nervous system (CNS)-active α1-adrenergic receptor antagonist, prazosin, would block the stress-induced increase in anxiety that occurs during alcohol deprivations. Methods Selectively bred male alcohol-preferring (P) rats were given three cycles of 5 days of ad libitum voluntary alcohol drinking interrupted by 2 days of alcohol deprivation, with or without 1 h of restraint stress 4 h after the start of each of the first two alcohol deprivation cycles. Prazosin (1.0 or 1.5 mg/kg, IP) or vehicle was administered before each restraint stress. Anxiety-like behavior during alcohol deprivation following the third 5-day cycle of alcohol drinking (7 days after the most recent restraint stress ± prazosin treatment) was measured by performance in an elevated plus-maze and in social approach/avoidance testing. Results Rats that received constant alcohol access, or alcohol access and deprivations without stress or prazosin treatments in the first two alcohol deprivations did not exhibit augmented anxiety-like behavior during the third deprivation. In contrast, rats that had been stressed during the first two alcohol deprivations exhibited increased anxiety-like behavior (compared with control rats) in both anxiety tests during the third deprivation. Prazosin given before stresses in the first two cycles of alcohol withdrawal prevented increased anxiety-like behavior during the third alcohol deprivation. Conclusion Prazosin treatment before stresses experienced during alcohol deprivations may prevent the increased anxiety during subsequent deprivation/abstinence that is a risk factor for relapse to alcohol drinking. Short summary Administration of prazosin before stresses during repetitive alcohol deprivations in male alcohol-preferring (P) rats prevents increased anxiety during a subsequent deprivation without further prazosin

  20. Isolation stress and chronic mild stress induced immobility in the defensive burying behavior and a transient increased ethanol intake in Wistar rats.

    PubMed

    Vázquez-León, Priscila; Martínez-Mota, Lucía; Quevedo-Corona, Lucía; Miranda-Páez, Abraham

    2017-09-01

    Stress can be experienced with or without adverse effects, of which anxiety and depression are two of the most important due to the frequent comorbidity with alcohol abuse in humans. Historically, stress has been considered a cause of drug use, particularly alcohol abuse due to its anxiolytic effects. In the present work we exposed male Wistar rats to two different stress conditions: single housing (social isolation, SI), and chronic mild stress (CMS). We compared both stressed groups to group-housed rats and rats without CMS (GH) to allow the determination of a clear behavioral response profile related to their respective endocrine stress response and alcohol intake pattern. We found that SI and CMS, to a greater extent, induced short-lasting increased sucrose consumption, a transient increase in serum corticosterone level, high latency/immobility, and low burying behavior in the defensive burying behavior (DBB) test, and a transient increase in alcohol intake. Thus, the main conclusion was that stress caused by both SI and CMS induced immobility in the DBB test and, subsequently, induced a transient increased voluntary ethanol intake in Wistar rats with a free-choice home-cage drinking paradigm. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. Identification of differentially expressed genes in Fiskeby III under ozone stress conditions

    USDA-ARS?s Scientific Manuscript database

    As the global climate changes, plants will be challenged by environmental stresses that are more extreme and more frequent leading to increased yield loss. Specifically, ozone stress is an increasing problem in both urban and rural areas. Soybeans are one of the plant species that are quite ozone se...

  2. Binary stress induces an increase in indole alkaloid biosynthesis in Catharanthus roseus

    PubMed Central

    Zhu, Wei; Yang, Bingxian; Komatsu, Setsuko; Lu, Xiaoping; Li, Ximin; Tian, Jingkui

    2015-01-01

    Catharanthus roseus is an important medicinal plant, which produces a variety of indole alkaloids of significant pharmaceutical relevance. In the present study, we aimed to investigate the potential stress-induced increase of indole alkaloid biosynthesis in C. roseus using proteomic technique. The contents of the detectable alkaloids ajmalicine, vindoline, catharanthine, and strictosidine in C. roseus were significantly increased under binary stress. Proteomic analysis revealed that the abundance of proteins related to tricarboxylic acid cycle and cell wall was largely increased; while, that of proteins related to tetrapyrrole synthesis and photosynthesis was decreased. Of note, 10-hydroxygeraniol oxidoreductase, which is involved in the biosynthesis of indole alkaloid was two-fold more abundant in treated group compared to the control. In addition, mRNA expression levels of genes involved in the indole alkaloid biosynthetic pathway indicated an up-regulation in their transcription in C. roseus under UV-B irradiation. These results suggest that binary stress might negatively affect the process of photosynthesis in C. roseus. In addition, the induction of alkaloid biosynthesis appears to be responsive to binary stress. PMID:26284098

  3. Pistil Smut Infection Increases Ovary Production, Seed Yield Components, and Pseudosexual Reproductive Allocation in Buffalograss

    PubMed Central

    Chandra, Ambika; Huff, David R.

    2014-01-01

    Sex expression of dioecious buffalograss [Bouteloua dactyloides Columbus (syn. Buchloë dactyloides (Nutt.) Engelm.)] is known to be environmentally stable with approximate 1:1, male to female, sex ratios. Here we show that infection by the pistil smut fungus [Salmacisia buchloëana Huff & Chandra (syn. Tilletia buchloëana Kellerman and Swingle)] shifts sex ratios of buffalograss to be nearly 100% phenotypically hermaphroditic. In addition, pistil smut infection decreased vegetative reproductive allocation, increased most seed yield components, and increased pseudosexual reproductive allocation in both sex forms compared to uninfected clones. In female sex forms, pistil smut infection resulted in a 26 fold increase in ovary production and a 35 fold increase in potential harvest index. In male sex forms, pistil smut infection resulted in 2.37 fold increase in floret number and over 95% of these florets contained a well-developed pistil. Although all ovaries of infected plants are filled with fungal teliospores and hence reproductively sterile, an average male-female pair of infected plants exhibited an 87 fold increase in potential harvest index compared to their uninfected clones. Acquiring an ability to mimic the effects of pistil smut infection would enhance our understanding of the flowering process in grasses and our efforts to increase seed yield of buffalograss and perhaps other grasses. PMID:27135522

  4. Climatic warming increases winter wheat yield but reduces grain nitrogen concentration in east China.

    PubMed

    Tian, Yunlu; Zheng, Chengyan; Chen, Jin; Chen, Changqing; Deng, Aixing; Song, Zhenwei; Zhang, Baoming; Zhang, Weijian

    2014-01-01

    Climatic warming is often predicted to reduce wheat yield and grain quality in China. However, direct evidence is still lacking. We conducted a three-year experiment with a Free Air Temperature Increase (FATI) facility to examine the responses of winter wheat growth and plant N accumulation to a moderate temperature increase of 1.5°C predicted to prevail by 2050 in East China. Three warming treatments (AW: all-day warming; DW: daytime warming; NW: nighttime warming) were applied for an entire growth period. Consistent warming effects on wheat plant were recorded across the experimental years. An increase of ca. 1.5°C in daily, daytime and nighttime mean temperatures shortened the length of pre-anthesis period averagely by 12.7, 8.3 and 10.7 d (P<0.05), respectively, but had no significant impact on the length of the post-anthesis period. Warming did not significantly alter the aboveground biomass production, but the grain yield was 16.3, 18.1 and 19.6% (P<0.05) higher in the AW, DW and NW plots than the non-warmed plot, respectively. Warming also significantly increased plant N uptake and total biomass N accumulation. However, warming significantly reduced grain N concentrations while increased N concentrations in the leaves and stems. Together, our results demonstrate differential impacts of warming on the depositions of grain starch and protein, highlighting the needs to further understand the mechanisms that underlie warming impacts on plant C and N metabolism in wheat.

  5. Stress relaxation of cell walls and the yield threshold for growth: demonstration and measurement by micro-pressure probe and psychrometer techniques.

    PubMed

    Cosgrove, D J; Van Volkenburgh, E; Cleland, R E

    1984-01-01

    Theory predicts that, for growing plant cells isolated from a supply of water, stress relaxation of the cell wall should decrease cell turgor pressure (P) until the yield threshold for cell explanation is reached. This prediction was tested by direct P measurements of pea (Pisum sativum L.) stem cortical cells before and after excision of the growing region and isolation of the growing tissue from an external water supply. Cell P was measured with the micro-pressure probe under conditions which eliminated transpiration. Psychrometric measurements of water potential confirmed the pressure-probe measurements. Following excision, P of the growing cells decreased in 1 h by an average of 1.8 bar to a mean plateau value of 2.8 bar, and remained constant thereafter. Treatment with 10(-5) M indole-3-acetic acid or 10(-5) M fusicoccin (known growth stimulants) accelerated the rate of P relaxation, whereas various treatments which inhibit growth slowed down or completely stopped P relaxation in apical segments. In contrast, P of basal (nongrowing) segments gradually increased because of absorption of solutes from the cell-wall free space of the tissue. Such solute absorption also occurred in apical segments, but wall relaxation held P at the yield threshold in those segments which were isolated from an external water supply. These results provide a new and rapid method for measuring the yield threshold and they show that P in intact growing pea stems exceeds the yield threshold by about 2 bar. Wall relaxation is shown here to affect the water potential and turgor pressure of excised growing segments. In addition, solute release and absorption upon excision may influence the water potential and turgor pressure of nongrowing excised plant tissues.

  6. High-Resolution Mapping of Yield Curve Shape and Evolution for Porous Rock: The Effect of Inelastic Compaction on Porous Bassanite

    NASA Astrophysics Data System (ADS)

    Bedford, John D.; Faulkner, Daniel R.; Leclère, Henri; Wheeler, John

    2018-02-01

    Porous rock deformation has important implications for fluid flow in a range of crustal settings as compaction can increase fluid pressure and alter permeability. The onset of inelastic strain for porous materials is typically defined by a yield curve plotted in differential stress (Q) versus effective mean stress (P) space. Empirical studies have shown that these curves are broadly elliptical in shape. Here conventional triaxial experiments are first performed to document (a) the yield curve of porous bassanite (porosity ≈ 27-28%), a material formed from the dehydration of gypsum, and (b) the postyield behavior, assuming that P and Q track along the yield surface as inelastic deformation accumulates. The data reveal that after initial yield, the yield surface cannot be perfectly elliptical and must evolve significantly as inelastic strain is accumulated. To investigate this further, a novel stress-probing methodology is developed to map precisely the yield curve shape and subsequent evolution for a single sample. These measurements confirm that the high-pressure side of the curve is partly composed of a near-vertical limb. Yield curve evolution is shown to be dependent on the nature of the loading path. Bassanite compacted under differential stress develops a heterogeneous microstructure and has a yield curve with a peak that is almost double that of an equal porosity sample that has been compacted hydrostatically. The dramatic effect of different loading histories on the strength of porous bassanite highlights the importance of understanding the associated microstructural controls on the nature of inelastic deformation in porous rock.

  7. Acute and chronic stress increase salivary cortisol: a study in the real-life setting of a national examination undertaken by medical graduates.

    PubMed

    González-Cabrera, J; Fernández-Prada, M; Iribar-Ibabe, C; Peinado, J M

    2014-03-01

    Spanish medical graduates who apply for a medical specialty training position (MIR) must take an examination that will shape their future personal and professional lives. Preparation for the test represents an important stressor that persists for several months. The aim of this study was to elucidate the stress pattern of this group and evaluate possible changes in the circadian rhythm of cortisol release in medical graduates preparing for this test. A repeated-measures longitudinal study was performed, measuring the salivary cortisol concentrations in 36 medical graduates (13 males and 23 females; mean age of 24.2 years) on five sampling days. Five cortisol samples were collected from 07:00 to 21:00 h in order to monitor changes in the circadian rhythm. On all sampling days (except on the day of the official examination), anxiety and psychological stress were evaluated with the Spanish versions of the State-Trait Anxiety Inventory (STAI) and the Perceived Stress Scale (PSS). During the study period, participants showed higher levels of anxiety than the Spanish reference population as well as a progressive increase in self-perceived stress. A significant increase in salivary cortisol concentration was observed in both chronic (study and examination preparation) and acute (examinations) situations. Our results suggest that the cortisol awakening response (CAR) may be a good indicator of anticipatory stress but is unaffected by long-term examination preparation. Comparison of results between the official examination day and the mock examination days yielded evidence that learning may modulate the behavior of the hypothalamic-pituitary-adrenal axis.

  8. Sequential ethanol fermentation and anaerobic digestion increases bioenergy yields from duckweed.

    PubMed

    Calicioglu, O; Brennan, R A

    2018-06-01

    The potential for improving bioenergy yields from duckweed, a fast-growing, simple, floating aquatic plant, was evaluated by subjecting the dried biomass directly to anaerobic digestion, or sequentially to ethanol fermentation and then anaerobic digestion, after evaporating ethanol from the fermentation broth. Bioethanol yields of 0.41 ± 0.03 g/g and 0.50 ± 0.01 g/g (glucose) were achieved for duckweed harvested from the Penn State Living-Filter (Lemna obscura) and Eco-Machine™ (Lemna minor/japonica and Wolffia columbiana), respectively. The highest biomethane yield, 390 ± 0.1 ml CH 4 /g volatile solids added, was achieved in a reactor containing fermented duckweed from the Living-Filter at a substrate-to-inoculum (S/I) ratio (i.e., duckweed to microorganism ratio) of 1.0. This value was 51.2% higher than the biomethane yield of a replicate reactor with raw (non-fermented) duckweed. The combined bioethanol-biomethane process yielded 70.4% more bioenergy from duckweed, than if anaerobic digestion had been run alone. Copyright © 2018 Elsevier Ltd. All rights reserved.

  9. Mapping Crop Yield and Sow Date Using High Resolution Imagery

    NASA Astrophysics Data System (ADS)

    Royal, K.

    2015-12-01

    Keitasha Royal, Meha Jain, Ph.D., David Lobell, Ph.D Mapping Crop Yield and Sow Date Using High Resolution ImageryThe use of satellite imagery in agriculture is becoming increasingly more significant and valuable. Due to the emergence of new satellites, such as Skybox, these satellites provide higher resolution imagery (e.g 1m) therefore improving the ability to map smallholder agriculture. For the smallholder farm dominated area of northern India, Skybox high-resolution satellite imagery can aid in understanding how to improve farm yields. In particular, we are interested in mapping winter wheat in India, as this region produces approximately 80% of the country's wheat crop, which is important given that wheat is a staple crop that provides approximately 20% of household calories. In northeast India, the combination of increased heat stress, limited irrigation access, and the difficulty for farmers to access advanced farming technologies results in farmers only producing about 50% of their potential crop yield. The use of satellite imagery can aid in understanding wheat yields through time and help identify ways to increase crop yields in the wheat belt of India. To translate Skybox satellite data into meaningful information about wheat fields, we examine vegetation indices, such as the normalized difference vegetation index (NDVI), to measure the "greenness" of plants to help determine the health of the crops. We test our ability to predict crop characteristics, like sow date and yield, using vegetation indices of 59 fields for which we have field data in Bihar, India.

  10. Estimating crop yields and crop evapotranspiration distributions from remote sensing and geospatial agricultural data

    NASA Astrophysics Data System (ADS)

    Smith, T.; McLaughlin, D.

    2017-12-01

    Growing more crops to provide a secure food supply to an increasing global population will further stress land and water resources that have already been significantly altered by agriculture. The connection between production and resource use depends on crop yields and unit evapotranspiration (UET) rates that vary greatly, over both time and space. For regional and global analyses of food security it is appropriate to treat yield and UET as uncertain variables conditioned on climatic and soil properties. This study describes how probability distributions of these variables can be estimated by combining remotely sensed land use and evapotranspiration data with in situ agronomic and soils data, all available at different resolutions and coverages. The results reveal the influence of water and temperature stress on crop yield at large spatial scales. They also provide a basis for stochastic modeling and optimization procedures that explicitly account for uncertainty in the environmental factors that affect food production.

  11. Cutaneous interstitial nitric oxide concentration does not increase during heat stress in humans

    NASA Technical Reports Server (NTRS)

    Crandall, C. G.; MacLean, D. A.

    2001-01-01

    Inhibition of cutaneous nitric oxide (NO) synthase reduces the magnitude of cutaneous vasodilation during whole body heating in humans. However, this observation is insufficient to conclude that NO concentration increases in the skin during a heat stress. This study was designed to test the hypothesis that whole body heating increases cutaneous interstitial NO concentration. This was accomplished by placing 2 microdialysis membranes in the forearm dermal space of 12 subjects. Both membranes were perfused with lactated Ringer solutions at a rate of 2 microl/min. In both normothermia and during whole body heating via a water perfused suit, dialysate from these membranes were obtained and analyzed for NO using the chemiluminescence technique. In six of these subjects, after the heat stress, the membranes were perfused with a 1 M solution of acetylcholine to stimulate NO release. Dialysate from these trials was also assayed to quantify cutaneous interstitial NO concentration. Whole body heating increased skin temperature from 34.6 +/- 0.2 to 38.8 +/- 0.2 degrees C (P < 0.05), which increased sublingual temperature (36.4 +/- 0.1 to 37.6 +/- 0.1 degrees C; P < 0.05), heart rate (63 +/- 5 to 93 +/- 5 beats/min; P < 0.05), and skin blood flow over the membranes (21 +/- 4 to 88 +/- 10 perfusion units; P < 0.05). NO concentration in the dialysate did not increase significantly during of the heat stress (7.6 +/- 0.7 to 8.6 +/- 0.8 microM; P > 0.05). After the heat stress, administration of acetylcholine in the perfusate significantly increased skin blood flow (128 +/- 6 perfusion units) relative to both normothermic and heat stress values and significantly increased NO concentration in the dialysate (15.8 +/- 2.4 microM). These data suggest that whole body heating does not increase cutaneous interstitial NO concentration in forearm skin. Rather, NO may serve in a permissive role in facilitating the effects of an unknown neurotransmitter, leading to cutaneous vasodilation

  12. Yield and yield gaps in central U.S. corn production systems

    USDA-ARS?s Scientific Manuscript database

    The magnitude of yield gaps (YG) (potential yield – farmer yield) provides some indication of the prospects for increasing crop yield. Quantile regression analysis was applied to county maize (Zea mays L.) yields (1972 – 2011) from Kentucky, Iowa and Nebraska (irrigated) (total of 115 counties) to e...

  13. A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa

    DOE PAGES

    Dalal, Jyoti; Lopez, Harry; Vasani, Naresh B.; ...

    2015-10-29

    Camelina sativa is an oilseed crop with great potential for biofuel production on marginal land. The seed oil from camelina has been converted to jet fuel and improved fuel efficiency in commercial and military test flights. Hydrogenation-derived renewable diesel from camelina is environmentally superior to that from canola due to lower agricultural inputs, and the seed meal is FDA approved for animal consumption. However, relatively low yield makes its farming less profitable. Our study is aimed at increasing camelina seed yield by reducing carbon loss from photorespiration via a photorespiratory bypass. Genes encoding three enzymes of the Escherichia coli glycolatemore » catabolic pathway were introduced: glycolate dehydrogenase (GDH), glyoxylate carboxyligase (GCL) and tartronic semialdehyde reductase (TSR). These enzymes compete for the photorespiratory substrate, glycolate, convert it to glycerate within the chloroplasts, and reduce photorespiration. As a by-product of the reaction, CO 2 is released in the chloroplast, which increases photosynthesis. Camelina plants were transformed with either partial bypass (GDH), or full bypass (GDH, GCL and TSR) genes. Furthermore, transgenic plants were evaluated for physiological and metabolic traits.« less

  14. A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Dalal, Jyoti; Lopez, Harry; Vasani, Naresh B.

    Camelina sativa is an oilseed crop with great potential for biofuel production on marginal land. The seed oil from camelina has been converted to jet fuel and improved fuel efficiency in commercial and military test flights. Hydrogenation-derived renewable diesel from camelina is environmentally superior to that from canola due to lower agricultural inputs, and the seed meal is FDA approved for animal consumption. However, relatively low yield makes its farming less profitable. Our study is aimed at increasing camelina seed yield by reducing carbon loss from photorespiration via a photorespiratory bypass. Genes encoding three enzymes of the Escherichia coli glycolatemore » catabolic pathway were introduced: glycolate dehydrogenase (GDH), glyoxylate carboxyligase (GCL) and tartronic semialdehyde reductase (TSR). These enzymes compete for the photorespiratory substrate, glycolate, convert it to glycerate within the chloroplasts, and reduce photorespiration. As a by-product of the reaction, CO 2 is released in the chloroplast, which increases photosynthesis. Camelina plants were transformed with either partial bypass (GDH), or full bypass (GDH, GCL and TSR) genes. Furthermore, transgenic plants were evaluated for physiological and metabolic traits.« less

  15. Climate change and maize yield in Iowa

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Xu, Hong; Twine, Tracy E.; Girvetz, Evan

    Climate is changing across the world, including the major maize-growing state of Iowa in the USA. To maintain crop yields, farmers will need a suite of adaptation strategies, and choice of strategy will depend on how the local to regional climate is expected to change. Here we predict how maize yield might change through the 21 st century as compared with late 20 th century yields across Iowa, USA, a region representing ideal climate and soils for maize production that contributes substantially to the global maize economy. To account for climate model uncertainty, we drive a dynamic ecosystem model withmore » output from six climate models and two future climate forcing scenarios. Despite a wide range in the predicted amount of warming and change to summer precipitation, all simulations predict a decrease in maize yields from late 20 th century to middle and late 21 st century ranging from 15% to 50%. Linear regression of all models predicts a 6% state-averaged yield decrease for every 1°C increase in warm season average air temperature. When the influence of moisture stress on crop growth is removed from the model, yield decreases either remain the same or are reduced, depending on predicted changes in warm season precipitation. Lastly, our results suggest that even if maize were to receive all the water it needed, under the strongest climate forcing scenario yields will decline by 10-20% by the end of the 21 st century.« less

  16. Climate change and maize yield in Iowa

    DOE PAGES

    Xu, Hong; Twine, Tracy E.; Girvetz, Evan

    2016-05-24

    Climate is changing across the world, including the major maize-growing state of Iowa in the USA. To maintain crop yields, farmers will need a suite of adaptation strategies, and choice of strategy will depend on how the local to regional climate is expected to change. Here we predict how maize yield might change through the 21 st century as compared with late 20 th century yields across Iowa, USA, a region representing ideal climate and soils for maize production that contributes substantially to the global maize economy. To account for climate model uncertainty, we drive a dynamic ecosystem model withmore » output from six climate models and two future climate forcing scenarios. Despite a wide range in the predicted amount of warming and change to summer precipitation, all simulations predict a decrease in maize yields from late 20 th century to middle and late 21 st century ranging from 15% to 50%. Linear regression of all models predicts a 6% state-averaged yield decrease for every 1°C increase in warm season average air temperature. When the influence of moisture stress on crop growth is removed from the model, yield decreases either remain the same or are reduced, depending on predicted changes in warm season precipitation. Lastly, our results suggest that even if maize were to receive all the water it needed, under the strongest climate forcing scenario yields will decline by 10-20% by the end of the 21 st century.« less

  17. Acid soil infertility effects on peanut yields and yield components

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Blamey, F.P.C.

    1983-01-01

    The interpretation of soil amelioration experiments with peanuts is made difficult by the unpredictibility of the crop and by the many factors altered when ameliorating acid soils. The present study was conducted to investigate the effects of lime and gypsum applications on peanut kernel yield via the three first order yield components, pods per ha, kernels per pod, and kernel mass. On an acid medium sandy loam soil (typic Plinthustult), liming resulted in a highly significant kernel yield increase of 117% whereas gypsum applications were of no significant benefit. As indicated by path coefficient analysis, an increase in the numbermore » of pods per ha was markedly more important in increasing yield than an increase in either the number of kernels per pod or kernel mass. Furthermore, exch. Al was found to be particularly detrimental to pod number. It was postulated that poor peanut yields resulting from acid soil infertility were mainly due to the depressive effect of exch. Al on pod number. Exch. Ca appeared to play a secondary role by ameliorating the adverse effects of exch. Al.« less

  18. A nonlinear viscoelastic constitutive equation - Yield predictions in multiaxial deformations

    NASA Technical Reports Server (NTRS)

    Shay, R. M., Jr.; Caruthers, J. M.

    1987-01-01

    Yield stress predictions of a nonlinear viscoelastic constitutive equation for amorphous polymer solids have been obtained and are compared with the phenomenological von Mises yield criterion. Linear viscoelasticity theory has been extended to include finite strains and a material timescale that depends on the instantaneous temperature, volume, and pressure. Results are presented for yield and the correct temperature and strain-rate dependence in a variety of multiaxial deformations. The present nonlinear viscoelastic constitutive equation can be formulated in terms of either a Cauchy or second Piola-Kirchhoff stress tensor, and in terms of either atmospheric or hydrostatic pressure.

  19. Targeting carbon for crop yield and drought resilience

    PubMed Central

    Griffiths, Cara A

    2017-01-01

    Abstract Current methods of crop improvement are not keeping pace with projected increases in population growth. Breeding, focused around key traits of stem height and disease resistance, delivered the step‐change yield improvements of the green revolution of the 1960s. However, subsequently, yield increases through conventional breeding have been below the projected requirement of 2.4% per year required by 2050. Genetic modification (GM) mainly for herbicide tolerance and insect resistance has been transformational, akin to a second green revolution, although GM has yet to make major inroads into intrinsic yield processes themselves. Drought imposes the major restriction on crop yields globally but, as yet, has not benefited substantially from genetic improvement and still presents a major challenge to agriculture. Much still has to be learnt about the complex process of how drought limits yield and what should be targeted. Mechanisms of drought adaptation from the natural environment cannot be taken into crops without significant modification for the agricultural environment because mechanisms of drought tolerance are often in contrast with mechanisms of high productivity required in agriculture. However, through convergence of fundamental and translational science, it would appear that a mechanism of sucrose allocation in crops can be modified for both productivity and resilience to drought and other stresses. Recent publications show how this mechanism can be targeted by GM, natural variation and a new chemical approach. Here, with an emphasis on drought, we highlight how understanding fundamental science about how crops grow, develop and what limits their growth and yield can be combined with targeted genetic selection and pioneering chemical intervention technology for transformational yield improvements. © 2017 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. PMID

  20. Characterization of five terminator regions that increase the protein yield of a transgene in Saccharomyces cerevisiae.

    PubMed

    Ito, Yoichiro; Yamanishi, Mamoru; Ikeuchi, Akinori; Imamura, Chie; Tokuhiro, Kenro; Kitagawa, Takao; Matsuyama, Takashi

    2013-12-01

    Strong terminator regions could be used to improve metabolically engineered yeasts by increasing the target enzyme protein yields above those achieved with traditional terminator regions. We recently identified five strong terminator regions (RPL41Bt, RPL15At, DIT1t, RPL3t, and IDP1t) in a comprehensive analysis of Saccharomyces cerevisiae. The effect of the terminator regions was analyzed by measuring the protein production of a linked transgene, and was shown to be twice that of a traditional terminator region (PGK1t). Here, we investigated whether the activity of the terminator regions is affected by exchange of a strong promoter or reporter in the linked transgene, carbon source for cell growth, stress factors, host yeast strain, or stage of the growth phase. Our results indicate that the activities of all five terminator regions were twice that of PGK1t in all conditions tested. In addition, we demonstrated that the strong activity of these terminator regions could be used to improve secretory production of endoglucanase II derived from Tricoderma ressei, and that the DIT1t strain was the best of the five strains for this purpose. We therefore propose that DIT1t, and the four other terminator regions, could be applied to the development of improved metabolically engineered yeasts. Copyright © 2013 Elsevier B.V. All rights reserved.

  1. New approach to increasing rice lodging resistance and biomass yield through the use of high gibberellin producing varieties.

    PubMed

    Okuno, Ayako; Hirano, Ko; Asano, Kenji; Takase, Wakana; Masuda, Reiko; Morinaka, Yoichi; Ueguchi-Tanaka, Miyako; Kitano, Hidemi; Matsuoka, Makoto

    2014-01-01

    Traditional breeding for high-yielding rice has been dependent on the widespread use of fertilizers and the cultivation of gibberellin (GA)-deficient semi-dwarf varieties. The use of semi-dwarf plants facilitates high grain yield since these varieties possess high levels of lodging resistance, and thus could support the high grain weight. Although this approach has been successful in increasing grain yield, it is desirable to further improve grain production and also to breed for high biomass. In this study, we re-examined the effect of GA on rice lodging resistance and biomass yield using several GA-deficient mutants (e.g. having defects in the biosynthesis or perception of GA), and high-GA producing line or mutant. GA-deficient mutants displayed improved bending-type lodging resistance due to their short stature; however they showed reduced breaking-type lodging resistance and reduced total biomass. In plants producing high amounts of GA, the bending-type lodging resistance was inferior to the original cultivars. The breaking-type lodging resistance was improved due to increased lignin accumulation and/or larger culm diameters. Further, these lines had an increase in total biomass weight. These results show that the use of rice cultivars producing high levels of GA would be a novel approach to create higher lodging resistance and biomass.

  2. Increasing correlations between personality traits and cortisol stress responses obtained by data aggregation.

    PubMed

    Pruessner, J C; Gaab, J; Hellhammer, D H; Lintz, D; Schommer, N; Kirschbaum, C

    1997-11-01

    Attempts to link personality traits and cortisol stress responses have often been inconclusive. The aim of this paper was to investigate this association by aggregating cortisol stress responses. Therefore, 20 healthy men were exposed to a task consisting of public speaking and mental arithmetics in front of an audience on five days. Six cortisol levels were measured in relation to the stressful task obtained at 10-min intervals on each day. Psychological assessment included the Questionnaire for Competence and Control (FKK) and the Giessen-Test (G-T). These questionnaires focus on assessing personality traits, i.e. locus of control and self-concept. Areas under the response curve (AUC) of the six cortisol samples were computed to obtain an index of the individual's cortisol stress response on each day. Since novelty is a random situational factor likely to mask individual differences in the stress response, the AUC cortisol stress responses of days two to five were consecutively aggregated, excluding the first day. Scales of the two questionnaires employed did not correlate with the AUC cortisol stress response of the first stress trial. The correlation pattern of the AUC cortisol measures of days two to five with the questionnaire scales was inconclusive. However, significant correlations emerged with an increasing number of cortisol stress responses aggregated. Correlations between the measure of social dominance and aggregated AUC cortisol stress responses rose from r = -.47 on day two of the experimental session to r = -.70 after aggregating days two to five. Similarly, measures of locus of control and cortisol stress responses became increasingly correlated with aggregation of several stress exposures. These data provide preliminary evidence for a relationship between questionnaire scales aiming at assessing personality traits and cortisol stress responses uncovered by repeated stress exposure and data aggregation. While novelty may mask the impact of

  3. Water deficit in field-grown Gossypium hirsutum primarily limits net photosynthesis by decreasing stomatal conductance, increasing photorespiration, and increasing the ratio of dark respiration to gross photosynthesis.

    PubMed

    Chastain, Daryl R; Snider, John L; Collins, Guy D; Perry, Calvin D; Whitaker, Jared; Byrd, Seth A

    2014-11-01

    Much effort has been expended to improve irrigation efficiency and drought tolerance of agronomic crops; however, a clear understanding of the physiological mechanisms that interact to decrease source strength and drive yield loss has not been attained. To elucidate the underlying mechanisms contributing to inhibition of net carbon assimilation under drought stress, three cultivars of Gossypium hirsutum were grown in the field under contrasting irrigation regimes during the 2012 and 2013 growing season near Camilla, Georgia, USA. Physiological measurements were conducted on three sample dates during each growing season (providing a broad range of plant water status) and included, predawn and midday leaf water potential (ΨPD and ΨMD), gross and net photosynthesis, dark respiration, photorespiration, and chlorophyll a fluorescence. End-of-season lint yield was also determined. ΨPD ranged from -0.31 to -0.95MPa, and ΨMD ranged from -1.02 to -2.67MPa, depending upon irrigation regime and sample date. G. hirsutum responded to water deficit by decreasing stomatal conductance, increasing photorespiration, and increasing the ratio of dark respiration to gross photosynthesis, thereby limiting PN and decreasing lint yield (lint yield declines observed during the 2012 growing season only). Conversely, even extreme water deficit, causing a 54% decline in PN, did not negatively affect actual quantum yield, maximum quantum yield, or photosynthetic electron transport. It is concluded that PN is primarily limited in drought-stressed G. hirsutum by decreased stomatal conductance, along with increases in respiratory and photorespiratory carbon losses, not inhibition or down-regulation of electron transport through photosystem II. It is further concluded that ΨPD is a reliable indicator of drought stress and the need for irrigation in field-grown cotton. Copyright © 2014 Elsevier GmbH. All rights reserved.

  4. The multitasking framework: the effects of increasing workload on acute psychobiological stress reactivity.

    PubMed

    Wetherell, Mark A; Carter, Kirsty

    2014-04-01

    A variety of techniques exist for eliciting acute psychological stress in the laboratory; however, they vary in terms of their ease of use, reliability to elicit consistent responses and the extent to which they represent the stressors encountered in everyday life. There is, therefore, a need to develop simple laboratory techniques that reliably elicit psychobiological stress reactivity that are representative of the types of stressors encountered in everyday life. The multitasking framework is a performance-based, cognitively demanding stressor, representative of environments where individuals are required to attend and respond to several different stimuli simultaneously with varying levels of workload. Psychological (mood and perceived workload) and physiological (heart rate and blood pressure) stress reactivity was observed in response to a 15-min period of multitasking at different levels of workload intensity in a sample of 20 healthy participants. Multitasking stress elicited increases in heart rate and blood pressure, and increased workload intensity elicited dose-response increases in levels of perceived workload and mood. As individuals rarely attend to single tasks in real life, the multitasking framework provides an alternative technique for modelling acute stress and workload in the laboratory. Copyright © 2013 John Wiley & Sons, Ltd.

  5. Unmanned aircraft system-derived crop height and normalized difference vegetation index metrics for sorghum yield and aphid stress assessment

    NASA Astrophysics Data System (ADS)

    Stanton, Carly; Starek, Michael J.; Elliott, Norman; Brewer, Michael; Maeda, Murilo M.; Chu, Tianxing

    2017-04-01

    A small, fixed-wing unmanned aircraft system (UAS) was used to survey a replicated small plot field experiment designed to estimate sorghum damage caused by an invasive aphid. Plant stress varied among 40 plots through manipulation of aphid densities. Equipped with a consumer-grade near-infrared camera, the UAS was flown on a recurring basis over the growing season. The raw imagery was processed using structure-from-motion to generate normalized difference vegetation index (NDVI) maps of the fields and three-dimensional point clouds. NDVI and plant height metrics were averaged on a per plot basis and evaluated for their ability to identify aphid-induced plant stress. Experimental soil signal filtering was performed on both metrics, and a method filtering low near-infrared values before NDVI calculation was found to be the most effective. UAS NDVI was compared with NDVI from sensors onboard a manned aircraft and a tractor. The correlation results showed dependence on the growth stage. Plot averages of NDVI and canopy height values were compared with per-plot yield at 14% moisture and aphid density. The UAS measures of plant height and NDVI were correlated to plot averages of yield and insect density. Negative correlations between aphid density and NDVI were seen near the end of the season in the most damaged crops.

  6. Using Satellite Data to Unpack Causes of Yield Gaps in India's Wheat Belt

    NASA Astrophysics Data System (ADS)

    Jain, M.; Singh, B.; Srivastava, A.; Malik, R. K.; McDonald, A.; Lobell, D. B.

    2016-12-01

    India will face significant food security challenges in the coming decades due to climate change, natural resource degradation, and population growth. Yields of wheat, one of India's staple crops, are already stagnating and will be significantly impacted by warming temperatures. Despite these challenges, wheat yields can be enhanced by implementing improved management in regions with existing yield gaps. To identify the magnitude and causes of current yield gaps, we produced 30 m resolution yield maps across India's main wheat belt, the Indo-Gangetic Plains (IGP), from 2000 to 2015. Yield maps were derived using a new method that translates satellite vegetation indices to yield estimates using crop model simulations, bypassing the need for ground calibration data that rarely exist in smallholder systems. We find that yields can be increased by 5% on average and up to 16% in the eastern IGP by improving management to current best practices within a given district. However, if policies and technologies are put in place to improve management to current best practices in Punjab, the highest yielding state, yields can be increased by 29% in the eastern IGP. Considering which factors most influence wheat yields, we find that later sow dates and warmer temperatures are most associated with low yields across the IGP. This suggests that strategies that reduce the negative effects of heat stress, like earlier sowing and planting heat-tolerant wheat varieties, are critical to India's current and future food security.

  7. The affection of the disturbance of the hydrodynamics of blood in case of stress on pathological increase of level of low density lipoproteins in blood. The formation of cylindrical plaques, and their participation in the development of acute ischemic disorders of heart and brain.

    PubMed

    Rusanov, S E

    2017-09-01

    In this article is given the new insight about the affection of stress on the increase of level of low density lipoproteins (LDL) in the blood, which is connected with the disturbance of hydrodynamics in the bloodstream, the attention was paid to the cylindrical cholesterol plaque, and it's classification. The disturbance of hydrodynamics of blood under the stress leads to the formation of a cylindrical cholesterol plaque, which repeats the contour of the vessel, and leads to the ischemic disorders of the heart and brain. The cylindrical cholesterol plaque goes through several stages of development: friable, yielding, dense, old. In the case of destruction of friable, fresh cholesterol plaque, releases a big quantity of low-density lipoproteins. This leads to the pathological increase of level of LDL in the blood. In the case of long disturbance of hydrodynamics, occurs the formation of strong links between low-density lipoproteins. Yielding cholesterol plaque is formed. Further maturation of cylindrical cholesterol plaque, leads to it's densifying and damage. We may emphasize, that short periods of strong contraction and expansion of vessels lead to the increase of level of LDL in the blood. Self-dependent restoration of normal level of LDL in blood occurs in the case of restoration of pressure in the limits of numbers, which are specific for particular person, and which don't exceed the physiological standard. Among patients with long duration of stress, the duration of vasospasm increases. LDL, without having a possibility to crumble, begin to stick together and form the yielding cylindrical plaque. It is characterized by having of not so strong connection with the vascular wall, and maintains only at the expanse of iteration of the vascular wall, it has cylindrical shape, is elastic and yellow. The thickness and length of walls depends on the degree of cross-clamping during the time of formation of yielding cylindrical plaque. In the case of stopping of spasm

  8. Increasing cancer detection yield of breast MRI using a new CAD scheme of mammograms

    NASA Astrophysics Data System (ADS)

    Tan, Maxine; Aghaei, Faranak; Hollingsworth, Alan B.; Stough, Rebecca G.; Liu, Hong; Zheng, Bin

    2016-03-01

    Although breast MRI is the most sensitive imaging modality to detect early breast cancer, its cancer detection yield in breast cancer screening is quite low (< 3 to 4% even for the small group of high-risk women) to date. The purpose of this preliminary study is to test the potential of developing and applying a new computer-aided detection (CAD) scheme of digital mammograms to identify women at high risk of harboring mammography-occult breast cancers, which can be detected by breast MRI. For this purpose, we retrospectively assembled a dataset involving 30 women who had both mammography and breast MRI screening examinations. All mammograms were interpreted as negative, while 5 cancers were detected using breast MRI. We developed a CAD scheme of mammograms, which include a new quantitative mammographic image feature analysis based risk model, to stratify women into two groups with high and low risk of harboring mammography-occult cancer. Among 30 women, 9 were classified into the high risk group by CAD scheme, which included all 5 women who had cancer detected by breast MRI. All 21 low risk women remained negative on the breast MRI examinations. The cancer detection yield of breast MRI applying to this dataset substantially increased from 16.7% (5/30) to 55.6% (5/9), while eliminating 84% (21/25) unnecessary breast MRI screenings. The study demonstrated the potential of applying a new CAD scheme to significantly increase cancer detection yield of breast MRI, while simultaneously reducing the number of negative MRIs in breast cancer screening.

  9. Synergistic interactions of ecosystem services: florivorous pest control boosts crop yield increase through insect pollination

    PubMed Central

    Albrecht, Matthias

    2016-01-01

    Insect pollination and pest control are pivotal functions sustaining global food production. However, they have mostly been studied in isolation and how they interactively shape crop yield remains largely unexplored. Using controlled field experiments, we found strong synergistic effects of insect pollination and simulated pest control on yield quantity and quality. Their joint effect increased yield by 23%, with synergistic effects contributing 10%, while their single contributions were 7% and 6%, respectively. The potential economic benefit for a farmer from the synergistic effects (12%) was 1.8 times greater than their individual contributions (7% each). We show that the principal underlying mechanism was a pronounced pest-induced reduction in flower lifetime, resulting in a strong reduction in the number of pollinator visits a flower receives during its lifetime. Our findings highlight the importance of non-additive interactions among ecosystem services (ES) when valuating, mapping or predicting them and reveal fundamental implications for ecosystem management and policy aimed at maximizing ES for sustainable agriculture. PMID:26865304

  10. Potassium and zinc increase tolerance to salt stress in wheat (Triticum aestivum L.).

    PubMed

    Jan, Amin Ullah; Hadi, Fazal; Midrarullah; Nawaz, Muhammad Asif; Rahman, Khaista

    2017-07-01

    Potassium and zinc are essential elements in plant growth and metabolism and plays a vital role in salt stress tolerance. To investigate the physiological mechanism of salt stress tolerance, a pot experiment was conducted. Potassium and zinc significantly minimize the oxidative stress and increase root, shoot and spike length in wheat varieties. Fresh and dry biomass were significantly increased by potassium followed by zinc as compared to control C. The photosynthetic pigment and osmolyte regulator (proline, total phenolic, and total carbohydrate) were significantly enhanced by potassium and zinc. Salt stress increases MDA content in wheat varieties while potassium and zinc counteract the adverse effect of salinity and significantly increased membrane stability index. Salt stress decreases the activities of antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase) while the exogenous application of potassium and zinc significantly enhanced the activities of these enzymes. A significant positive correlation was found of spike length with proline (R 2  = 0.966 ∗∗∗ ), phenolic (R 2  = 0.741 ∗ ) and chlorophyll (R 2  = 0.853 ∗∗ ). The MDA content showed significant negative correlation (R 2  = 0.983 ∗∗∗ ) with MSI. It is concluded that potassium and zinc reduced toxic effect of salinity while its combine application showed synergetic effect and significantly enhanced salt tolerance. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  11. Pregestational stress attenuated fertility rate in dams and increased seizure susceptibility in offspring.

    PubMed

    Mahmoodkhani, Maryam; Saboory, Ehsan; Roshan-Milani, Shiva; Azizi, Negar; Karimipour, Mojtaba; Rasmi, Yosef; Gholinejad, Zafar

    2018-02-01

    Many studies have found that stress during pregnancy is linked to an increased incidence of epileptic behaviors and reproductive disorders. However, few works have investigated the effect of pregestational stress on seizure susceptibility in the offspring. We investigated the effect of pregestational stress on epileptic behaviors in the offspring as well as fertility rate in dams. The male and female rats were randomly divided into four groups to form a combination of control and stressed groups for each sex. The rats were subjected to predatory stress (exposed to a cat) twice per day for 50 (male) and 15 (female) consecutive days. At the end of the stress procedure, the rats were coupled as follows: both male and female control (M C -F C ), male stressed/female control (M S -F C ), male control/female stressed (M C -F S ), and both male and female stressed (M S -F S ). Then, the puppies born from these groups were counted and evaluated for pentylentetrazole (PTZ)-induced seizure. There was no significant difference between the male and female pups in each identical group in terms of litter size and epileptic behaviors, except duration of tail rigidity and duration of immobility. The total score of seizure increased in all the stressed groups, but more severely in the M S -F S group. However, the onset of the first epileptic behavior and tonic-clonic seizure significantly decreased in the stressed groups. Moreover, fertility rate significantly decreased in the stressed groups compared with the control group, but there was no significant difference in terms of litter size between the groups. These data revealed the impact of pregestational stress during spermatogenesis and oogenesis on fertility rate in dams and epileptic behaviors in the offspring. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Arabidopsis Enhanced Drought Tolerance1/HOMEODOMAIN GLABROUS11 Confers Drought Tolerance in Transgenic Rice without Yield Penalty1[W][OA

    PubMed Central

    Yu, Linhui; Chen, Xi; Wang, Zhen; Wang, Shimei; Wang, Yuping; Zhu, Qisheng; Li, Shigui; Xiang, Chengbin

    2013-01-01

    Enhancing drought tolerance without yield decrease has been a great challenge in crop improvement. Here, we report the Arabidopsis (Arabidopsis thaliana) homodomain-leucine zipper transcription factor Enhanced Drought Tolerance/HOMEODOMAIN GLABROUS11 (EDT1/HDG11) was able to confer drought tolerance and increase grain yield in transgenic rice (Oryza sativa) plants. The improved drought tolerance was associated with a more extensive root system, reduced stomatal density, and higher water use efficiency. The transgenic rice plants also had higher levels of abscisic acid, proline, soluble sugar, and reactive oxygen species-scavenging enzyme activities during stress treatments. The increased grain yield of the transgenic rice was contributed by improved seed setting, larger panicle, and more tillers as well as increased photosynthetic capacity. Digital gene expression analysis indicated that AtEDT1/HDG11 had a significant influence on gene expression profile in rice, which was consistent with the observed phenotypes of transgenic rice plants. Our study shows that AtEDT1/HDG11 can improve both stress tolerance and grain yield in rice, demonstrating the efficacy of AtEDT1/HDG11 in crop improvement. PMID:23735506

  13. Heat stress attenuates the increase in arterial blood pressure during the cold pressor test.

    PubMed

    Cui, Jian; Shibasaki, Manabu; Low, David A; Keller, David M; Davis, Scott L; Crandall, Craig G

    2010-11-01

    The mechanisms by which heat stress impairs the control of blood pressure leading to compromised orthostatic tolerance are not thoroughly understood. A possible mechanism may be an attenuated blood pressure response to a given increase in sympathetic activity. This study tested the hypothesis that whole body heating attenuates the blood pressure response to a non-baroreflex-mediated sympathoexcitatory stimulus. Ten healthy subjects were instrumented for the measurement of integrated muscle sympathetic nerve activity (MSNA), mean arterial blood pressure (MAP), heart rate, sweat rate, and forearm skin blood flow. Subjects were exposed to a cold pressor test (CPT) by immersing a hand in an ice water slurry for 3 min while otherwise normothermic and while heat stressed (i.e., increase core temperature ~0.7°C via water-perfused suit). Mean responses from the final minute of the CPT were evaluated. In both thermal conditions CPT induced significant increases in MSNA and MAP without altering heart rate. Although the increase in MSNA to the CPT was similar between thermal conditions (normothermia: Δ14.0 ± 2.6; heat stress: Δ19.1 ± 2.6 bursts/min; P = 0.09), the accompanying increase in MAP was attenuated when subjects were heat stressed (normothermia: Δ25.6 ± 2.3, heat stress: Δ13.4 ± 3.0 mmHg; P < 0.001). The results demonstrate that heat stress can attenuate the pressor response to a sympathoexcitatory stimulus.

  14. Studies of the physical, yield and failure behavior of aliphatic polyketones

    NASA Astrophysics Data System (ADS)

    Karttunen, Nicole Renee

    This thesis describes an investigation into the multiaxial yield and failure behavior of an aliphatic polyketone terpolymer. The behavior is studied as a function of: stress state, strain rate, temperature, and sample processing conditions. Results of this work include: elucidation of the behavior of a recently commercialized polymer, increased understanding of the effects listed above, insight into the effects of processing conditions on the morphology of the polyketone, and a description of yield strength of this material as a function of stress state, temperature, and strain rate. The first portion of work focuses on the behavior of a set of samples that are extruded under "common" processing conditions. Following this reference set of tests, the effect of testing this material at different temperatures is studied. A total of four different temperatures are examined. In addition, the effect of altering strain rate is examined. Testing is performed under pseudo-strain rate control at constant nominal octahedral shear strain rate for each failure envelope. A total of three different rates are studied. An extension of the first portion of work involves modeling the yield envelope. This is done by combining two approaches: continuum level and molecular level. The use of both methods allows the description of the yield envelope as a function of stress state, strain rate and temperature. The second portion of work involves the effects of processing conditions. For this work, additional samples are extruded with different shear and thermal histories than the "standard" material. One set of samples is processed with shear rates higher and lower than the standard. A second set is processed at higher and lower cooling rates than the standard. In order to understand the structural cause for changes in behavior with processing conditions, morphological characterization is performed on these samples. In particular, the effect on spherulitic structure is important. Residual

  15. Future Climate CO2 Levels Mitigate Stress Impact on Plants: Increased Defense or Decreased Challenge?

    PubMed Central

    AbdElgawad, Hamada; Zinta, Gaurav; Beemster, Gerrit T. S.; Janssens, Ivan A.; Asard, Han

    2016-01-01

    Elevated atmospheric CO2 can stimulate plant growth by providing additional C (fertilization effect), and is observed to mitigate abiotic stress impact. Although, the mechanisms underlying the stress mitigating effect are not yet clear, increased antioxidant defenses, have been held primarily responsible (antioxidant hypothesis). A systematic literature analysis, including “all” papers [Web of Science (WoS)-cited], addressing elevated CO2 effects on abiotic stress responses and antioxidants (105 papers), confirms the frequent occurrence of the stress mitigation effect. However, it also demonstrates that, in stress conditions, elevated CO2 is reported to increase antioxidants, only in about 22% of the observations (e.g., for polyphenols, peroxidases, superoxide dismutase, monodehydroascorbate reductase). In most observations, under stress and elevated CO2 the levels of key antioxidants and antioxidant enzymes are reported to remain unchanged (50%, e.g., ascorbate peroxidase, catalase, ascorbate), or even decreased (28%, e.g., glutathione peroxidase). Moreover, increases in antioxidants are not specific for a species group, growth facility, or stress type. It seems therefore unlikely that increased antioxidant defense is the major mechanism underlying CO2-mediated stress impact mitigation. Alternative processes, probably decreasing the oxidative challenge by reducing ROS production (e.g., photorespiration), are therefore likely to play important roles in elevated CO2 (relaxation hypothesis). Such parameters are however rarely investigated in connection with abiotic stress relief. Understanding the effect of elevated CO2 on plant growth and stress responses is imperative to understand the impact of climate changes on plant productivity. PMID:27200030

  16. Method and apparatus for increasing the durability and yield of thin film photovoltaic devices

    DOEpatents

    Phillips, James E.; Lasswell, Patrick G.

    1987-01-01

    Thin film photovoltaic cells having a pair of semiconductor layers between an opaque and a transparent electrical contact are manufactured in a method which includes the step of scanning one of the semiconductor layers to determine the location of any possible shorting defect. Upon the detection of such defect, the defect is eliminated to increase the durability and yield of the photovoltaic device.

  17. LACIE: Wheat yield models for the USSR

    NASA Technical Reports Server (NTRS)

    Sakamoto, C. M.; Leduc, S. K.

    1977-01-01

    A quantitative model determining the relationship between weather conditions and wheat yield in the U.S.S.R. was studied to provide early reliable forecasts on the size of the U.S.S.R. wheat harvest. Separate models are developed for spring wheat and for winter. Differences in yield potential and responses to stress conditions and cultural improvements necessitate models for each class.

  18. Stress Increases the Negative Effects of Chronic Pain on Hippocampal Neurogenesis.

    PubMed

    Romero-Grimaldi, Carmen; Berrocoso, Esther; Alba-Delgado, Cristina; Madrigal, Jose Luis M; Perez-Nievas, Beatriz G; Leza, Juan Carlos; Mico, Juan Antonio

    2015-10-01

    Patients with chronic pain often suffer from affective disorders and cognitive decline, which significantly impairs their quality of life. In addition, many of these patients also experience stress unrelated to their illness, which can aggravate their symptoms. These nociceptive inputs are received by the hippocampus, in which maladaptive neuroplastic changes may occur in the conditions of chronic pain. The hippocampus is a structure involved in emotionality, learning, and memory, and the proliferating cells in the granular layer of the hippocampal dentate gyrus respond to chronic pain by slowing their turnover. However, whether the maturation, survival, and integration of newborn cells in the hippocampus are affected by chronic pain remains unclear. In addition, it is unknown whether an added stress may increase this effect. We have evaluated the proliferation, differentiation, and survival of newborn hippocampal cells in a rat model of neuropathic pain (chronic constriction injury), with or without stress (chronic immobilization), by assessing the incorporation of bromodeoxyuridine into proliferating cells and immunostaining. The data obtained indicated that there was a decrease in the number of proliferating cells 8 days after nerve injury in animals subjected to neuropathic pain, an effect that was exacerbated by stress. Moreover, 4 weeks after nerve injury, neuropathic pain was associated with a loss of neuroblasts and the reduced survival of new mature neurons in the hippocampal granular layer, phenomena that also were increased by stress. By contrast, the rate of differentiation was not affected in this paradigm. Neuropathic pain negatively influences hippocampal neurogenesis (proliferation and survival), and this effect is exacerbated by stress. These neuroplastic changes may account for the affective and cognitive impairment seen in patients with chronic pain.

  19. Modelling of loading, stress relaxation and stress recovery in a shape memory polymer.

    PubMed

    Sweeney, J; Bonner, M; Ward, I M

    2014-09-01

    A multi-element constitutive model for a lactide-based shape memory polymer has been developed that represents loading to large tensile deformations, stress relaxation and stress recovery at 60, 65 and 70°C. The model consists of parallel Maxwell arms each comprising neo-Hookean and Eyring elements. Guiu-Pratt analysis of the stress relaxation curves yields Eyring parameters. When these parameters are used to define the Eyring process in a single Maxwell arm, the resulting model yields at too low a stress, but gives good predictions for longer times. Stress dip tests show a very stiff response on unloading by a small strain decrement. This would create an unrealistically high stress on loading to large strain if it were modelled by an elastic element. Instead it is modelled by an Eyring process operating via a flow rule that introduces strain hardening after yield. When this process is incorporated into a second parallel Maxwell arm, there results a model that fully represents both stress relaxation and stress dip tests at 60°C. At higher temperatures a third arm is required for valid predictions. Crown Copyright © 2014. Published by Elsevier Ltd. All rights reserved.

  20. Stress in biological invasions: Introduced invasive grey squirrels increase physiological stress in native Eurasian red squirrels.

    PubMed

    Santicchia, Francesca; Dantzer, Ben; van Kesteren, Freya; Palme, Rupert; Martinoli, Adriano; Ferrari, Nicola; Wauters, Lucas Armand

    2018-05-23

    Invasive alien species can cause extinction of native species through processes including predation, interspecific competition for resources or disease-mediated competition. Increases in stress hormones in vertebrates may be associated with these processes and contribute to the decline in survival or reproduction of the native species. Eurasian red squirrels (Sciurus vulgaris) have gone extinct across much of the British Isles and parts of Northern Italy following the introduction of North American invasive grey squirrels (Sciurus carolinensis). We extracted glucocorticoid metabolites from faecal samples to measure whether the presence of the invasive species causes an increase in physiological stress in individuals of the native species. We show that native red squirrels in seven sites where they co-occurred with invasive grey squirrels had glucocorticoid concentrations that were three times higher than those in five sites without the invasive species. Moreover, in a longitudinal study, stress hormones in native red squirrels increased after colonisation by grey squirrels. When we experimentally reduced the abundance of the invasive grey squirrels, the concentration of faecal glucocorticoid metabolites in co-occurring red squirrels decreased significantly between pre- and postremoval periods. Hence, we found that the invasive species acts as a stressor which significantly increases the concentrations of glucocorticoids in the native species. Given that sustained elevations in glucocorticoids could reduce body growth and reproductive rate, our results are consistent with previous studies where the co-occurrence of the invasive grey squirrel was associated with smaller size and lower reproductive output in red squirrels. © 2018 The Authors. Journal of Animal Ecology © 2018 British Ecological Society.

  1. Heat and drought stresses in crops and approaches for their mitigation

    NASA Astrophysics Data System (ADS)

    Lamaoui, Mouna; Jemo, Martin; Datla, Raju; Bekkaoui, Faouzi

    2018-02-01

    Drought and heat are major abiotic stresses that reduce crop productivity and weaken global food security, especially given the current and growing impacts of climate change and increases in the occurrence and severity of both stress factors. Plants have developed dynamic responses at the morphological, physiological and biochemical levels allowing them to escape and/or adapt to unfavourable environmental conditions. Nevertheless, even the mildest heat and drought stress negatively affects crop yield. Further, several independent studies have shown that increased temperature and drought can reduce crop yields by as much as 50%. Response to stress is complex and involves several factors including signaling, transcription factors, hormones, and secondary metabolites. The reproductive phase of development, leading to the grain production is shown to be more sensitive to heat stress in several crops. Advances coming from biotechnology including progress in genomics and information technology may mitigate the detrimental effects of heat and drought through the use of agronomic management practices and the development of crop varieties with increased productivity under stress. This review presents recent progress in key areas relevant to plant drought and heat tolerance. Furthermore, an overview and implications of physiological, biochemical and genetic aspects in the context of heat and drought are presented. Potential strategies to improve crop productivity are discussed.

  2. Heat and Drought Stresses in Crops and Approaches for Their Mitigation.

    PubMed

    Lamaoui, Mouna; Jemo, Martin; Datla, Raju; Bekkaoui, Faouzi

    2018-01-01

    Drought and heat are major abiotic stresses that reduce crop productivity and weaken global food security, especially given the current and growing impacts of climate change and increases in the occurrence and severity of both stress factors. Plants have developed dynamic responses at the morphological, physiological and biochemical levels allowing them to escape and/or adapt to unfavorable environmental conditions. Nevertheless, even the mildest heat and drought stress negatively affects crop yield. Further, several independent studies have shown that increased temperature and drought can reduce crop yields by as much as 50%. Response to stress is complex and involves several factors including signaling, transcription factors, hormones, and secondary metabolites. The reproductive phase of development, leading to the grain production is shown to be more sensitive to heat stress in several crops. Advances coming from biotechnology including progress in genomics and information technology may mitigate the detrimental effects of heat and drought through the use of agronomic management practices and the development of crop varieties with increased productivity under stress. This review presents recent progress in key areas relevant to plant drought and heat tolerance. Furthermore, an overview and implications of physiological, biochemical and genetic aspects in the context of heat and drought are presented. Potential strategies to improve crop productivity are discussed.

  3. Influence of genotype, floral stage, and water stress on floral nectar yield and composition of mānuka (Leptospermum scoparium).

    PubMed

    Clearwater, Michael J; Revell, Maria; Noe, Stevie; Manley-Harris, Merilyn

    2018-03-05

    Floral nectar can be variable in composition, influencing pollinator behaviour and the composition of honey derived from it. The non-peroxide antibacterial activity of mānuka (Leptospermum scoparium, Myrtaceae) honey results from the chemical conversion of the triose sugar dihydroxyacetone (DHA), after DHA accumulates for an unknown reason in the nectar. This study examined variation in nectar DHA, glucose, fructose and sucrose content with floral stage of development, between mānuka genotypes with differing flower morphology, and in response to water stress. Six mānuka genotypes were grown without nectar-feeding insects. Stages of flower development were defined, nectar was harvested and its composition was compared between stages and genotypes, and with floral morphology. Water stress was imposed and its effect on nectar composition was examined. Nectar was present from soon after flower opening until the end of petal abscission, with the quantity of accumulated nectar sugars rising, then stabilizing or falling, indicating nectar secretion followed by reabsorption in some genotypes. The quantity of DHA, the ratio of DHA to other nectar sugars and the fructose to glucose ratio also varied with stage of development, indicating differences in rates of production and reabsorption between nectar components. Nectar composition and yield per flower also differed between genotypes, although neither was positively related to nectary area or stomatal density. Drying soil had no effect on nectar composition or yield, but variation in nectar yield was correlated with temperature prior to nectar sampling. Mānuka nectar yield and composition are strongly influenced by plant genotype, flower age and the environment. There were clear stoichiometric relationships between glucose, fructose and sucrose per flower, but DHA per flower was only weakly correlated with the amount of other sugars, suggesting that accumulation of the triose sugar is indirectly coupled to secretion of

  4. An alternative approach for modeling strength differential effect in sheet metals with symmetric yield functions

    NASA Astrophysics Data System (ADS)

    Kurukuri, Srihari; Worswick, Michael J.

    2013-12-01

    An alternative approach is proposed to utilize symmetric yield functions for modeling the tension-compression asymmetry commonly observed in hcp materials. In this work, the strength differential (SD) effect is modeled by choosing separate symmetric plane stress yield functions (for example, Barlat Yld 2000-2d) for the tension i.e., in the first quadrant of principal stress space, and compression i.e., third quadrant of principal stress space. In the second and fourth quadrants, the yield locus is constructed by adopting interpolating functions between uniaxial tensile and compressive stress states. In this work, different interpolating functions are chosen and the predictive capability of each approach is discussed. The main advantage of this proposed approach is that the yield locus parameters are deterministic and relatively easy to identify when compared to the Cazacu family of yield functions commonly used for modeling SD effect observed in hcp materials.

  5. A meteorologically-driven yield reduction model for spring and winter wheat

    NASA Technical Reports Server (NTRS)

    Ravet, F. W.; Cremins, W. J.; Taylor, T. W.; Ashburn, P.; Smika, D.; Aaronson, A. (Principal Investigator)

    1983-01-01

    A yield reduction model for spring and winter wheat was developed for large-area crop condition assessment. Reductions are expressed in percentage from a base yield and are calculated on a daily basis. The algorithm contains two integral components: a two-layer soil water budget model and a crop calendar routine. Yield reductions associated with hot, dry winds (Sukhovey) and soil moisture stress are determined. Input variables include evapotranspiration, maximum temperature and precipitation; subsequently crop-stage, available water holding percentage and stress duration are evaluated. No specific base yield is required and may be selected by the user; however, it may be generally characterized as the maximum likely to be produced commercially at a location.

  6. Dietary-induced binge eating increases prefrontal cortex neural activation to restraint stress and increases binge food consumption following chronic guanfacine.

    PubMed

    Bello, Nicholas T; Walters, Amy L; Verpeut, Jessica L; Caverly, Jonathan

    2014-10-01

    Binge eating is a prominent feature of bulimia nervosa and binge eating disorder. Stress or perceived stress is an often-cited reason for binge eating. One notion is that the neural pathways that overlap with stress reactivity and feeding behavior are altered by recurrent binge eating. Using young adult female rats in a dietary-induced binge eating model (30 min access to binge food with or without 24-h calorie restriction, twice a week, for 6 weeks) we measured the neural activation by c-Fos immunoreactivity to the binge food (vegetable shortening mixed with 10% sucrose) in bingeing and non-bingeing animals under acute stress (immobilization; 1 h) or no stress conditions. There was an increase in the number of immunopositive cells in the dorsal medial prefrontal cortex (mPFC) in stressed animals previously exposed to the binge eating feeding schedules. Because attention deficit hyperactive disorder (ADHD) medications target the mPFC and have some efficacy at reducing binge eating in clinical populations, we examined whether chronic (2 weeks; via IP osmotic mini-pumps) treatment with a selective alpha-2A adrenergic agonist (0.5 mg/kg/day), guanfacine, would reduce binge-like eating. In the binge group with only scheduled access to binge food (30 min; twice a week; 8 weeks), guanfacine increased total calories consumed during the 30-min access period from the 2-week pre-treatment baseline and increased binge food consumption compared with saline-treated animals. These experiments suggest that mPFC is differentially activated in response to an immobilization stress in animals under different dietary conditions and chronic guanfacine, at the dose tested, was ineffective at reducing binge-like eating. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. Dietary Supplementation of Chromium Can Alleviate Negative Impacts of Heat Stress on Performance, Carcass Yield, and Some Blood Hematology and Chemistry Indices of Growing Japanese Quail.

    PubMed

    El-Kholy, Mohamed Soliman; El-Hindawy, Mohamed Mohamed; Alagawany, Mahmoud; Abd El-Hack, Mohamed Ezzat; El-Sayed, Sabry Abd El-Gawad Abd El-Halim

    2017-09-01

    The main objective of this work was to investigate the impact of dietary chromium supplementation on growth indices, carcass yield, and some hematological and biochemical blood parameters of growing Japanese quails subjected to heat stress. A total of 360 unsexed 2-week-old Japanese quail chicks were used in a 2 × 3 factorial arrangement that had two ambient temperatures (23 ± 2 and 33 ± 2 °C) and three dietary chromium (0.00, 500, and 1000 μg Cr/kg diet as chromium picolinate). For induction of heat stress, the room temperature was set at 33 ± 2 °C from 2 to 6 weeks of age. Results showed that body weight, body weight gain, and feed intake were decreased for birds subjected to heat stress condition during 2 to 4 and 2 to 6 weeks of age. Feed conversion was not significantly (P > 0.05) affected by high ambient temperature throughout the experiment. Carcass, dressing, liver, and heart percentages were not influenced by the ambient temperature or dietary chromium or their combinations. Significant temperature × chromium combinations were observed for hemoglobin value (P = 0.025) and packed cell volume (P = 0.001). Cholesterol and glucose in plasma were increased (P = 0.004 or 0.022) in quails subjected to heat stress condition. Plasma measurements of total proteins, albumin, globulin, lipids, glucose, and A/G ratio of quail chicks were not influenced (P > 0.05) by chromium, while cholesterol was increased with increasing chromium level (P = 0.033). High ambient temperature or dietary chromium levels or their interactions did not (P > 0.05) affect plasma levels of aspartate transaminase (AST), alanine transaminase (ALT), triiodothyronine (T3), thyroxin (T4), or T3/T4 ratio of growing quails. From these observations, it can be concluded that dietary chromium supplementation of growing Japanese quail subjected to heat stress condition could beneficially affect growth performance and carcasses, as well as modulate the hematological

  8. Increased saccharification yields from aspen biomass upon treatment with enzymatically generated peracetic acid.

    PubMed

    Duncan, Shona; Jing, Qing; Katona, Adrian; Kazlauskas, Romas J; Schilling, Jonathan; Tschirner, Ulrike; Aldajani, Waleed Wafa

    2010-03-01

    The recalcitrance of lignocellulosic biomass to enzymatic release of sugars (saccharification) currently limits its use as feedstock for biofuels. Enzymatic hydrolysis of untreated aspen wood releases only 21.8% of the available sugars due primarily to the lignin barrier. Nature uses oxidative enzymes to selectively degrade lignin in lignocellulosic biomass, but thus far, natural enzymes have been too slow for industrial use. In this study, oxidative pretreatment with commercial peracetic acid (470 mM) removed 40% of the lignin (from 19.9 to 12.0 wt.% lignin) from aspen and enhanced the sugar yields in subsequent enzymatic hydrolysis to about 90%. Increasing the amount of lignin removed correlated with increasing yields of sugar release. Unfortunately, peracetic acid is expensive, and concentrated forms can be hazardous. To reduce costs and hazards associated with using commercial peracetic acid, we used a hydrolase to catalyze the perhydrolysis of ethyl acetate generating 60-70 mM peracetic acid in situ as a pretreatment to remove lignin from aspen wood. A single pretreatment was insufficient, but multiple cycles (up to eight) removed up to 61.7% of the lignin enabling release of >90% of the sugars during saccharification. This value corresponds to a predicted 581 g of fermentable sugars from 1 kg of aspen wood. Improvements in the enzyme stability are needed before the enzymatically generated peracetic acid is a commercially viable alternative.

  9. Ozone Exposure Increases Circulating Stress Hormones and Lipid Metabolites in Humans

    PubMed Central

    Miller, Desinia B.; Ghio, Andrew J.; Karoly, Edward D.; Bell, Lauren N.; Snow, Samantha J.; Madden, Michael C.; Soukup, Joleen; Cascio, Wayne E.; Gilmour, M. Ian

    2016-01-01

    Rationale: Air pollution has been associated with increased prevalence of type 2 diabetes; however, the mechanisms remain unknown. We have shown that acute ozone exposure in rats induces release of stress hormones, hyperglycemia, leptinemia, and glucose intolerance that are associated with global changes in peripheral glucose, lipid, and amino acid metabolism. Objectives: To examine ozone-induced metabolic derangement in humans using serum metabolomic assessment, establish human-to-rodent coherence, and identify novel nonprotein biomarkers. Methods: Serum samples were obtained from a crossover clinical study that included two clinic visits (n = 24 each) where each subject was blindly exposed in the morning to either filtered air or 0.3 parts per million ozone for 2 hours during 15-minute on-off exercise. Serum samples collected within 1 hour after exposure were assessed for changes in metabolites using a metabolomic approach. Measurements and Main Results: Metabolomic analysis revealed that ozone exposure markedly increased serum cortisol and corticosterone together with increases in monoacylglycerol, glycerol, and medium- and long-chain free fatty acids, reflective of lipid mobilization and catabolism. Additionally, ozone exposure increased serum lysolipids, potentially originating from membrane lipid breakdown. Ozone exposure also increased circulating mitochondrial β-oxidation–derived metabolites, such as acylcarnitines, together with increases in the ketone body 3-hydroxybutyrate. These changes suggested saturation of β-oxidation by ozone in exercising humans. Conclusions: As in rodents, acute ozone exposure increased stress hormones and globally altered peripheral lipid metabolism in humans, likely through activation of a neurohormonally mediated stress response pathway. The metabolomic assessment revealed new biomarkers and allowed for establishment of rodent-to-human coherence. Clinical trial registered with www.clinicaltrials.gov (NCT 01492517

  10. Academic stress and personality interact to increase the neural response to high-calorie food cues.

    PubMed

    Neseliler, Selin; Tannenbaum, Beth; Zacchia, Maria; Larcher, Kevin; Coulter, Kirsty; Lamarche, Marie; Marliss, Errol B; Pruessner, Jens; Dagher, Alain

    2017-09-01

    Psychosocial stress is associated with an increased intake of palatable foods and weight gain in stress-reactive individuals. Personality traits have been shown to predict stress-reactivity. However, it is not known if personality traits influence brain activity in regions implicated in appetite control during psychosocial stress. The current study assessed whether Gray's Behavioural Inhibition System (BIS) scale, a measure of stress-reactivity, was related to the activity of brain regions implicated in appetite control during a stressful period. Twenty-two undergraduate students participated in a functional magnetic resonance imaging (fMRI) experiment once during a non-exam period and once during final exams in a counter-balanced order. In the scanner, they viewed food and scenery pictures. In the exam compared with the non-exam condition, BIS scores related to increased perceived stress and correlated with increased blood-oxygen-level dependent (BOLD) response to high-calorie food images in regions implicated in food reward and subjective value, such as the ventromedial prefrontal cortex, (vmPFC) and the amygdala. BIS scores negatively related to the functional connectivity between the vmPFC and the dorsolateral prefrontal cortex. The results demonstrate that the BIS trait influences stress reactivity. This is observed both as an increased activity in brain regions implicated in computing the value of food cues and decreased connectivity of these regions to prefrontal regions implicated in self-control. This suggests that the effects of real life stress on appetitive brain function and self-control is modulated by a personality trait. This may help to explain why stressful periods can lead to overeating in vulnerable individuals. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Development of heat and drought related extreme weather events and their effect on winter wheat yields in Germany

    NASA Astrophysics Data System (ADS)

    Lüttger, Andrea B.; Feike, Til

    2018-04-01

    Climate change constitutes a major challenge for high productivity in wheat, the most widely grown crop in Germany. Extreme weather events including dry spells and heat waves, which negatively affect wheat yields, are expected to aggravate in the future. It is crucial to improve the understanding of the spatiotemporal development of such extreme weather events and the respective crop-climate relationships in Germany. Thus, the present study is a first attempt to evaluate the historic development of relevant drought and heat-related extreme weather events from 1901 to 2010 on county level (NUTS-3) in Germany. Three simple drought indices and two simple heat stress indices were used in the analysis. A continuous increase in dry spells over time was observed over the investigated periods from 1901-1930, 1931-1960, 1961-1990 to 2001-2010. Short and medium dry spells, i.e., precipitation-free periods longer than 5 and 8 days, respectively, increased more strongly compared to longer dry spells (longer than 11 days). The heat-related stress indices with maximum temperatures above 25 and 28 °C during critical wheat growth phases showed no significant increase over the first three periods but an especially sharp increase in the final 1991-2010 period with the increases being particularly pronounced in parts of Southwestern Germany. Trend analysis over the entire 110-year period using Mann-Kendall test revealed a significant positive trend for all investigated indices except for heat stress above 25 °C during flowering period. The analysis of county-level yield data from 1981 to 2010 revealed declining spatial yield variability and rather constant temporal yield variability over the three investigated (1981-1990, 1991-2000, and 2001-2010) decades. A clear spatial gradient manifested over time with variability in the West being much smaller than in the east of Germany. Correlating yield variability with the previously analyzed extreme weather indices revealed strong

  12. Stressful life events increase aggression and alcohol use in young carriers of the GABRA2 rs279826/rs279858 A-allele.

    PubMed

    Kiive, Evelyn; Laas, Kariina; Vaht, Mariliis; Veidebaum, Toomas; Harro, Jaanus

    2017-08-01

    Research of GABRA2 gene in alcohol use and impulse control suggests its role in aggressive behaviour. The purpose of the present study was to examine the effects of GABRA2 genotype and stressful life events on aggressive behaviour, alcohol use frequency and occurrence of alcohol use disorder in a population representative sample of adolescents followed up from third grade to 25 years of age. The sample consisted of the younger cohort of the longitudinal Estonian Children Personality, Behaviour and Health Study. Aggressive behaviour was rated with the activity scale of af Klinteberg, Illinois Bully Scale and Buss-Perry Aggression Questionnaire. Stressful life events and alcohol use were self-reported. Life history of aggression and lifetime occurrence of psychiatric disorders were estimated in a structured interview. The sample was genotyped for GABRA2 rs279826 and rs279858 polymorphisms that are in strong linkage disequilibrium and yielded very similar findings: Higher number of stressful life events reported at age 15 was associated with increased fighting in A-allele carriers, but not in GG homozygotes. At age 25, A-allele carriers with more stressful life events scored higher on physical aggression than those with less stress, and this was also observed regarding life history of aggression. A-allele carriers exposed to higher stress had consumed alcoholic beverages more frequently at age 15, and by age 25, they had alcohol use disorder with higher prevalence. The results of the present study suggest that the GABRA2 genotype interacts with stress in young people with impact on the development of alcohol use and aggressive behaviour. Copyright © 2017 Elsevier B.V. and ECNP. All rights reserved.

  13. Historical gains in soybean (Glycine max Merr.) seed yield are driven by linear increases in light interception, energy conversion, and partitioning efficiencies

    PubMed Central

    Koester, Robert P.; Skoneczka, Jeffrey A.; Cary, Troy R.; Diers, Brian W.; Ainsworth, Elizabeth A.

    2014-01-01

    Soybean (Glycine max Merr.) is the world’s most widely grown leguminous crop and an important source of protein and oil for food and feed. Soybean yields have increased substantially throughout the past century, with yield gains widely attributed to genetic advances and improved cultivars as well as advances in farming technology and practice. Yet, the physiological mechanisms underlying the historical improvements in soybean yield have not been studied rigorously. In this 2-year experiment, 24 soybean cultivars released between 1923 and 2007 were grown in field trials. Physiological improvements in the efficiencies by which soybean canopies intercepted light (εi), converted light energy into biomass (εc), and partitioned biomass into seed (εp) were examined. Seed yield increased by 26.5kg ha–1 year–1, and the increase in seed yield was driven by improvements in all three efficiencies. Although the time to canopy closure did not change in historical soybean cultivars, extended growing seasons and decreased lodging in more modern lines drove improvements in εi. Greater biomass production per unit of absorbed light resulted in improvements in εc. Over 84 years of breeding, soybean seed biomass increased at a rate greater than total aboveground biomass, resulting in an increase in εp. A better understanding of the physiological basis for yield gains will help to identify targets for soybean improvement in the future. PMID:24790116

  14. Microdomain Yield Behaviour in an Ultra-High Strength Low Alloy Steel for Marine Use with Low Sensitivity of SCC

    NASA Astrophysics Data System (ADS)

    Yin, Jiang; Tao, Anxiang; Xu, Pingguang; Ping, Dehai

    The present paper involves a fundamental research on microdomain yield behavior of an ultrahigh strength low alloy steel with high temperature tempered bainite. The smooth cylinder specimen was took from deep water mooring chain links from the steel with the chemical composition of 0.23C-0.25Si -0.70Mn-3.55 (Cr+Ni+Mo) -0.13 (V+Nb+Ti) (mass %) ,which was quenched from 1253K and then tempered at 873K Its macroscopic yield strength is 1120MPa and the tensile strength is 1250MPa In-situ neutron diffraction measurements of loading tension have suggested that a good linear elastic deformation can be kept up to 500MPa stress, and then (200) priority non-linear elastic strain, that is the yield of crystal lattice occur at 700MPa and the (110) non-linear elastic strain was found at 800MPa. The (200) and (110) nonlinear elastic strain increases gradually when the stress was further increased, however, the (211) kept its linear elastic deformation stage as before. The sub-microstructural analysis carried out using TEM and additional determine the nature and quantitative analysis has revealed that there are three kinds of alloy carbides: (1) θ-M3C cementites with an average particle size of less than 50 nm which inside laths and lath boundaries; (2) ɛ-M2C formed uniformly within the ferrites with a length of less than 200 nm and width of less than 20 nm; (3) ultra-fine high density MC cohered with matrix α-Fe and its particle size is about 2 nm. The whole microdomain yield behaviour of the material was possibly influenced by the fcc-MC with high density. The results of CLT (constant load), SSRT (slow strain rate) and KIscc test of the present chain in seawater solution indicate, that threshold value of SCC (stress corrosion cracking) stress exceed 0.8 tensile strength and the chain's KIscc value is double of KIscc value of 4340 steel type parts. MC not only form strong hydrogen trap, but also slow down microdomain yield likely by means of increasing yield strength of

  15. Method and apparatus for increasing the durability and yield of thin film photovoltaic devices

    DOEpatents

    Phillips, J.E.; Lasswell, P.G.

    1987-02-03

    Thin film photovoltaic cells having a pair of semiconductor layers between an opaque and a transparent electrical contact are manufactured in a method which includes the step of scanning one of the semiconductor layers to determine the location of any possible shorting defect. Upon the detection of such defect, the defect is eliminated to increase the durability and yield of the photovoltaic device. 10 figs.

  16. Social Stress Increases Cortisol and Hampers Attention in Adolescents with Excess Weight

    PubMed Central

    Verdejo-Garcia, Antonio; Moreno-Padilla, Maria; Garcia-Rios, M. Carmen; Lopez-Torrecillas, Francisca; Delgado-Rico, Elena; Schmidt-Rio-Valle, Jacqueline; Fernandez-Serrano, Maria J.

    2015-01-01

    Objective To experimentally examine if adolescents with excess weight are more sensitive to social stress and hence more sensitive to harmful effects of stress in cognition. Design and Methods We conducted an experimental study in 84 adolescents aged 12 to 18 years old classified in two groups based on age adjusted Body Mass Index percentile: Normal weight (n=42) and Excess weight (n=42). Both groups were exposed to social stress as induced by the virtual reality version of the Trier Social Stress Task --participants were requested to give a public speech about positive and negative aspects of their personalities in front of a virtual audience. The outcome measures were salivary cortisol levels and performance in cognitive tests before and after the social stressor. Cognitive tests included the CANTAB Rapid Visual Processing Test (measuring attention response latency and discriminability) and the Iowa Gambling Task (measuring decision-making). Results Adolescents with excess weight compared to healthy weight controls displayed increased cortisol response and less improvement of attentional performance after the social stressor. Decision-making performance decreased after the social stressor in both groups. Conclusion Adolescents who are overweight or obese have increased sensitivity to social stress, which detrimentally impacts attentional skills. PMID:25898204

  17. Abiotic Stresses Shift Belowground Populus-Associated Bacteria Toward a Core Stress Microbiome

    PubMed Central

    Carter, Kelsey R.; Carrell, Alyssa A.; Jun, Se-Ran; Jawdy, Sara S.; Vélez, Jessica M.; Gunter, Lee E.; Yang, Zamin; Nookaew, Intawat; Engle, Nancy L.; Lu, Tse-Yuan S.; Schadt, Christopher W.; Tschaplinski, Timothy J.; Tuskan, Gerald A.; Pelletier, Dale A.; Weston, David J.

    2018-01-01

    ABSTRACT Adverse growth conditions can lead to decreased plant growth, productivity, and survival, resulting in poor yields or failure of crops and biofeedstocks. In some cases, the microbial community associated with plants has been shown to alleviate plant stress and increase plant growth under suboptimal growing conditions. A systematic understanding of how the microbial community changes under these conditions is required to understand the contribution of the microbiome to water utilization, nutrient uptake, and ultimately yield. Using a microbiome inoculation strategy, we studied how the belowground microbiome of Populus deltoides changes in response to diverse environmental conditions, including water limitation, light limitation (shading), and metal toxicity. While plant responses to treatments in terms of growth, photosynthesis, gene expression and metabolite profiles were varied, we identified a core set of bacterial genera that change in abundance in response to host stress. The results of this study indicate substantial structure in the plant microbiome community and identify potential drivers of the phytobiome response to stress. IMPORTANCE The identification of a common “stress microbiome” indicates tightly controlled relationships between the plant host and bacterial associates and a conserved structure in bacterial communities associated with poplar trees under different growth conditions. The ability of the microbiome to buffer the plant from extreme environmental conditions coupled with the conserved stress microbiome observed in this study suggests an opportunity for future efforts aimed at predictably modulating the microbiome to optimize plant growth. PMID:29404422

  18. Abiotic Stresses Shift Belowground Populus -Associated Bacteria Toward a Core Stress Microbiome

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Timm, Collin M.; Carter, Kelsey R.; Carrell, Alyssa A.

    Adverse growth conditions can lead to decreased plant growth, productivity, and survival, resulting in poor yields or failure of crops and biofeedstocks. In some cases, the microbial community associated with plants has been shown to alleviate plant stress and increase plant growth under suboptimal growing conditions. A systematic understanding of how the microbial community changes under these conditions is required to understand the contribution of the microbiome to water utilization, nutrient uptake, and ultimately yield. Using a microbiome inoculation strategy, we studied how the belowground microbiome ofPopulus deltoideschanges in response to diverse environmental conditions, including water limitation, light limitation (shading),more » and metal toxicity. While plant responses to treatments in terms of growth, photosynthesis, gene expression and metabolite profiles were varied, we identified a core set of bacterial genera that change in abundance in response to host stress. The results of this study indicate substantial structure in the plant microbiome community and identify potential drivers of the phytobiome response to stress.The identification of a common “stress microbiome” indicates tightly controlled relationships between the plant host and bacterial associates and a conserved structure in bacterial communities associated with poplar trees under different growth conditions. The ability of the microbiome to buffer the plant from extreme environmental conditions coupled with the conserved stress microbiome observed in this study suggests an opportunity for future efforts aimed at predictably modulating the microbiome to optimize plant growth.« less

  19. Abiotic Stresses Shift Belowground Populus -Associated Bacteria Toward a Core Stress Microbiome

    DOE PAGES

    Timm, Collin M.; Carter, Kelsey R.; Carrell, Alyssa A.; ...

    2018-01-23

    Adverse growth conditions can lead to decreased plant growth, productivity, and survival, resulting in poor yields or failure of crops and biofeedstocks. In some cases, the microbial community associated with plants has been shown to alleviate plant stress and increase plant growth under suboptimal growing conditions. A systematic understanding of how the microbial community changes under these conditions is required to understand the contribution of the microbiome to water utilization, nutrient uptake, and ultimately yield. Using a microbiome inoculation strategy, we studied how the belowground microbiome ofPopulus deltoideschanges in response to diverse environmental conditions, including water limitation, light limitation (shading),more » and metal toxicity. While plant responses to treatments in terms of growth, photosynthesis, gene expression and metabolite profiles were varied, we identified a core set of bacterial genera that change in abundance in response to host stress. The results of this study indicate substantial structure in the plant microbiome community and identify potential drivers of the phytobiome response to stress.The identification of a common “stress microbiome” indicates tightly controlled relationships between the plant host and bacterial associates and a conserved structure in bacterial communities associated with poplar trees under different growth conditions. The ability of the microbiome to buffer the plant from extreme environmental conditions coupled with the conserved stress microbiome observed in this study suggests an opportunity for future efforts aimed at predictably modulating the microbiome to optimize plant growth.« less

  20. Breeding high-yielding drought-tolerant rice: genetic variations and conventional and molecular approaches

    PubMed Central

    Kumar, Arvind; Dixit, Shalabh; Ram, T.; Yadaw, R. B.; Mishra, K. K.; Mandal, N. P.

    2014-01-01

    The increased occurrence and severity of drought stress have led to a high yield decline in rice in recent years in drought-affected areas. Drought research at the International Rice Research Institute (IRRI) over the past decade has concentrated on direct selection for grain yield under drought. This approach has led to the successful development and release of 17 high-yielding drought-tolerant rice varieties in South Asia, Southeast Asia, and Africa. In addition to this, 14 quantitative trait loci (QTLs) showing a large effect against high-yielding drought-susceptible popular varieties were identified using grain yield as a selection criterion. Six of these (qDTY 1.1, qDTY 2.2, qDTY 3.1, qDTY 3.2, qDTY 6.1, and qDTY 12.1) showed an effect against two or more high-yielding genetic backgrounds in both the lowland and upland ecosystem, indicating their usefulness in increasing the grain yield of rice under drought. The yield of popular rice varieties IR64 and Vandana has been successfully improved through a well-planned marker-assisted backcross breeding approach, and QTL introgression in several other popular varieties is in progress. The identification of large-effect QTLs for grain yield under drought and the higher yield increase under drought obtained through the use of these QTLs (which has not been reported in other cereals) indicate that rice, because of its continuous cultivation in two diverse ecosystems (upland, drought tolerant, and lowland, drought susceptible), has benefited from the existence of larger genetic variability than in other cereals. This can be successfully exploited using marker-assisted breeding. PMID:25205576

  1. Inoculant of Arbuscular Mycorrhizal Fungi (Rhizophagus clarus) Increase Yield of Soybean and Cotton under Field Conditions.

    PubMed

    Cely, Martha V T; de Oliveira, Admilton G; de Freitas, Vanessa F; de Luca, Marcelo B; Barazetti, André R; Dos Santos, Igor M O; Gionco, Barbara; Garcia, Guilherme V; Prete, Cássio E C; Andrade, Galdino

    2016-01-01

    Nutrient availability is an important factor in crop production, and regular addition of chemical fertilizers is the most common practice to improve yield in agrosystems for intensive crop production. The use of some groups of microorganisms that have specific activity providing nutrients to plants is a good alternative, and arbuscular mycorrhizal fungi (AMF) enhance plant nutrition by providing especially phosphorus, improving plant growth and increasing crop production. Unfortunately, the use of AMF as an inoculant on a large scale is not yet widely used, because of several limitations in obtaining a large amount of inoculum due to several factors, such as low growth, the few species of AMF domesticated under in vitro conditions, and high competition with native AMF. The objective of this work was to test the infectivity of a Rhizophagus clarus inoculum and its effectiveness as an alternative for nutrient supply in soybean (Glycine max L.) and cotton (Gossypium hirsutum L.) when compared with conventional chemical fertilization under field conditions. The experiments were carried out in a completely randomized block design with five treatments: Fertilizer, AMF, AMF with Fertilizer, AMF with 1/2 Fertilizer, and the Control with non-inoculated and non-fertilized plants. The parameters evaluated were AMF root colonization and effect of inoculation on plant growth, nutrient absorption and yield. The results showed that AMF inoculation increased around 20 % of root colonization in both soybean and cotton; nutrients analyses in vegetal tissues showed increase of P and nitrogen content in inoculated plants, these results reflect in a higher yield. Our results showed that, AMF inoculation increase the effectiveness of fertilizer application in soybean and reduce the fertilizer dosage in cotton.

  2. Synergistic interactions of ecosystem services: florivorous pest control boosts crop yield increase through insect pollination.

    PubMed

    Sutter, Louis; Albrecht, Matthias

    2016-02-10

    Insect pollination and pest control are pivotal functions sustaining global food production. However, they have mostly been studied in isolation and how they interactively shape crop yield remains largely unexplored. Using controlled field experiments, we found strong synergistic effects of insect pollination and simulated pest control on yield quantity and quality. Their joint effect increased yield by 23%, with synergistic effects contributing 10%, while their single contributions were 7% and 6%, respectively. The potential economic benefit for a farmer from the synergistic effects (12%) was 1.8 times greater than their individual contributions (7% each). We show that the principal underlying mechanism was a pronounced pest-induced reduction in flower lifetime, resulting in a strong reduction in the number of pollinator visits a flower receives during its lifetime. Our findings highlight the importance of non-additive interactions among ecosystem services (ES) when valuating, mapping or predicting them and reveal fundamental implications for ecosystem management and policy aimed at maximizing ES for sustainable agriculture. © 2016 The Author(s).

  3. Effects of concentrations of sodium chloride on photosynthesis, antioxidative enzymes, growth and fiber yield of hybrid ramie.

    PubMed

    Huang, Chengjian; Wei, Gang; Jie, Yucheng; Wang, Longchang; Zhou, Hangfei; Ran, Chunyan; Huang, Zaocun; Jia, Huijuan; Anjum, Shakeel Ahmad

    2014-03-01

    Ramie (Boehmeria nivea L.) is one of the oldest and most important fiber crops in China due to the comfortable textile of its fine fiber. Increased ramie fiber demand brings ramie cultivation to salt-affected regions. The aim of this research was to determine morphological, physiological and biochemical responses of ramie by subjecting plants to varying concentrations of NaCl (0, 2, 4, 6 and 8 g NaCl/kg dry soil) at vigorous growth stage for 10 and 20 days. Results indicated that salinity stress substantially inhibited the growth of hybrid ramie plants and led to remarkable decline in fiber yield. However, when grown at 2 g NaCl/kg growth and fiber yield were similar to non-saline control. In addition, chlorophyll fluorescence and gas exchange parameters were correlated with growth and yield response. Salt treatments promoted a subsequent decrease in maximum quantum efficiency of PSII photochemistry (Fv/Fm), quantum efficiency of open PSII reaction centers (Fv'/Fm') and quantum yield of PSII (φPSII) while non-photochemical quenching (NPQ) changed conversely. Photochemical quenching (qP) and electron transport rate of PSII (ETR) increased at 2 and 4 g NaCl/kg then decreased at 6 and 8 g NaCl/kg. Substantial decline in the PSII activity at high salinity was associated with the loss of chlorophyll contents. Moreover, marked decrease in net photosynthetic rate (A), transpiration rate (E), stomatal conductance (gs) was also recorded. Nonetheless, intercellular CO2 (Ci) decreased at low salt stress, subsequently increased at high salt stress while water use efficiency (WUE) and instantaneous water use efficiency (WUEi) altered in opposite direction. Substantial decrease of photosynthesis at high salinity was due to non-stomatal factors. Furthermore, salinity stress led to decrease of proteins and accumulation of proline and malondialdehyde (MDA), as well as enhanced activities of superoxide dismutase (SOD, EC 1.15.1.1) and peroxidase (POD, EC 1.11.1.6), whereas

  4. Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

    PubMed Central

    Zhao, X.; Moates, G.K.; Elliston, A.; Wilson, D.R.; Coleman, M.J.; Waldron, K.W.

    2015-01-01

    This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210 °C, 10 min) and then subjected to simultaneous saccharification and fermentation (SSF) using Cellic® CTec 2 (20 U or 0.87 FPU g−1 substrate) cellulase plus β-glucosidase (2 U g−1 substrate) and a yeast inoculum of 10% (v/v or 8.0 × 107 cells mL−1). At a substrate concentration of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concentration of 20% (w/v) by metabolising fermentation inhibitors. PMID:26210138

  5. The Evolution of Elastic Moduli With Increasing Crack Damage During Cyclic Stressing of Etna Basalt

    NASA Astrophysics Data System (ADS)

    Heap, M. J.; Meredith, P. G.; Vinciguerra, S.; Boon, S. A.

    2007-12-01

    Volcanic edifices, such as Mt. Etna volcano, are commonly subject to cycles of pressurization and depressurization over extended periods of time due to repeated episodes of magma emplacement from deep reservoirs to shallow depths. Such repeated episodes of deformation can lead to an increase in the level of crack damage within the rocks of the edifice, and hence changes in their elastic properties. Importantly, a number of volcano monitoring techniques, such as seismic tomography and ground deformation modeling, rely on accurate knowledge of elastic properties. However, the effect of cyclic stressing on mechanical and elastic properties of volcanic rock remains unclear. To this end, we report results of changes in elastic moduli from stress-cycling experiments on samples of extrusive basalt from Mount Etna, Italy. The basalt contains an extensive pre-existing network of isotropic, interconnected microcracks caused by cooling. Both oven-dry and water-saturated samples were initially loaded to 20 MPa at a constant rate and then unloaded to 8 MPa. Samples were then sequentially reloaded and unloaded at the same rate with the peak stress in each subsequent cycle increased by 10 MPa. Stress-cycling was continued until each sample failed. Results from oven-dry samples showed a gradual reduction in sample stiffness with each increasing stress cycle that resulted in a total decrease in Young's modulus of approximately 30% and an increase in Poisson's ratio of approximately 60%. Results from water-saturated samples showed an almost identical trend. These changes in moduli are attributed to the growth of new cracks in each stress cycle and, hence, an increase in the total crack density. This is supported by the observation of increased acoustic emission (micro-seismic) output in each cycle. We also observed the Kaiser stress-memory effect, where acoustic emission on each cycle only occurs when the maximum stress in the previous cycle has been exceeded. During the deformation

  6. On cyclic yield strength in definition of limits for characterisation of fatigue and creep behaviour

    NASA Astrophysics Data System (ADS)

    Gorash, Yevgen; MacKenzie, Donald

    2017-06-01

    This study proposes cyclic yield strength as a potential characteristic of safe design for structures operating under fatigue and creep conditions. Cyclic yield strength is defined on a cyclic stress-strain curve, while monotonic yield strength is defined on a monotonic curve. Both values of strengths are identified using a two-step procedure of the experimental stress-strain curves fitting with application of Ramberg-Osgood and Chaboche material models. A typical S-N curve in stress-life approach for fatigue analysis has a distinctive minimum stress lower bound, the fatigue endurance limit. Comparison of cyclic strength and fatigue limit reveals that they are approximately equal. Thus, safe fatigue design is guaranteed in the purely elastic domain defined by the cyclic yielding. A typical long-term strength curve in time-to-failure approach for creep analysis has two inflections corresponding to the cyclic and monotonic strengths. These inflections separate three domains on the long-term strength curve, which are characterised by different creep fracture modes and creep deformation mechanisms. Therefore, safe creep design is guaranteed in the linear creep domain with brittle failure mode defined by the cyclic yielding. These assumptions are confirmed using three structural steels for normal and high-temperature applications. The advantage of using cyclic yield strength for characterisation of fatigue and creep strength is a relatively quick experimental identification. The total duration of cyclic tests for a cyclic stress-strain curve identification is much less than the typical durations of fatigue and creep rupture tests at the stress levels around the cyclic yield strength.

  7. Corticosterone mediates stress-related increased intestinal permeability in a region-specific manner

    PubMed Central

    Zheng, Gen; Wu, Shu-Pei; Hu, Yongjun; Smith, David E; Wiley, John W.; Hong, Shuangsong

    2012-01-01

    Background Chronic psychological stress (CPS) is associated with increased intestinal epithelial permeability and visceral hyperalgesia. It is unknown whether corticosterone (CORT) plays a role in mediating alterations of epithelial permeability in response to CPS. Methods Male rats were subjected to 1-hour water avoidance (WA) stress or subcutaneous CORT injection daily for 10 consecutive days in the presence or absence of corticoid-receptor antagonist RU-486. The visceromotor response (VMR) to colorectal distension (CRD) was measured. The in situ single-pass intestinal perfusion was used to measure intestinal permeability in jejunum and colon simultaneously. Key Results We observed significant decreases in the levels of glucocorticoid receptor (GR) and tight junction proteins in the colon but not the jejunum in stressed rats. These changes were largely reproduced by serial CORT injections in control rats and were significantly reversed by RU-486. Stressed and CORT-injected rats demonstrated a 3-fold increase in permeability for PEG-400 (MW) in colon but not jejunum and significant increase in VMR to CRD, which was significantly reversed by RU-486. In addition, no differences in permeability to PEG-4,000 and PEG-35,000 were detected between control and WA groups. Conclusions & Inferences Our findings indicate that CPS was associated with region-specific decrease in epithelial tight junction protein levels in the colon, increased colon epithelial permeability to low-molecular weight macromolecules which were largely reproduced by CORT treatment in control rats and prevented by RU-486. These observations implicate a novel, region-specific role for CORT as a mediator of CPS-induced increased permeability to macromolecules across the colon epithelium. PMID:23336591

  8. Chronic social stress increases nitric oxide-dependent vasorelaxation in normotensive rats

    PubMed Central

    Puzserova, Angelika; Bernatova, Iveta

    2010-01-01

    The aim of this study was to examine oxidative load and endothelium-dependent vasorelaxation in the serotonin pre-constricted femoral artery (FA) of Wistar-Kyoto (WKY) rats exposed to chronic social stress produced by crowding in the presence or absence of ascorbic acid (AsA) in working solution. Adult male rats were randomly divided into control (living space: 480 cm2/rat) or stressed (living space: 200 cm2/rat) groups for 8 weeks. Blood pressure and heart rate, determined using tail-cuff plethysmography, were not influenced by stress vs. control. Conjugated dienes (CD) and concentrations of thiobarbituric acid-reactive substances (TBARS) were measured in the left ventricle and liver (for assessment of oxidative load) and were found unchanged by chronic crowding. The nitric oxide (NO)-dependent component of endothelium-dependent relaxation was investigated in the FA using a wire myograph. In both the presence and absence of AsA, acetylcholine-induced relaxation of the FA of stressed rats significantly exceeded that of the controls, which was associated with an increase of the NO-dependent component. In conclusion, the data showed that chronic crowding did not produce oxidative stress in the organs investigated and indicate that elevation of NO production during chronic stress is an important way of adaptation, which may prevent normotensive rats from the development of stress-induced hypertension. PMID:21331175

  9. Molecular Based Temperature and Strain Rate Dependent Yield Criterion for Anisotropic Elastomeric Thin Films

    NASA Technical Reports Server (NTRS)

    Bosi, F.; Pellegrino, S.

    2017-01-01

    A molecular formulation of the onset of plasticity is proposed to assess temperature and strain rate effects in anisotropic semi-crystalline rubbery films. The presented plane stress criterion is based on the strain rate-temperature superposition principle and the cooperative theory of yielding, where some parameters are assumed to be material constants, while others are considered to depend on specific modes of deformation. An orthotropic yield function is developed for a linear low density polyethylene thin film. Uniaxial and biaxial inflation experiments were carried out to determine the yield stress of the membrane via a strain recovery method. It is shown that the 3% offset method predicts the uniaxial elastoplastic transition with good accuracy. Both the tensile yield points along the two principal directions of the film and the biaxial yield stresses are found to obey the superposition principle. The proposed yield criterion is compared against experimental measurements, showing excellent agreement over a wide range of deformation rates and temperatures.

  10. Association of Beta-Glucan Endogenous Production with Increased Stress Tolerance of Intestinal Lactobacilli▿

    PubMed Central

    Stack, Helena M.; Kearney, Niamh; Stanton, Catherine; Fitzgerald, Gerald F.; Ross, R. Paul

    2010-01-01

    The exopolysaccharide beta-glucan has been reported to be associated with many health-promoting and prebiotic properties. The membrane-associated glycosyltransferase enzyme (encoded by the gtf gene), responsible for microbial beta-glucan production, catalyzes the conversion of sugar nucleotides into beta-glucan. In this study, the gtf gene from Pediococcus parvulus 2.6 was heterologously expressed in Lactobacillus paracasei NFBC 338. When grown in the presence of glucose (7%, wt/vol), the recombinant strain (pNZ44-GTF+) displayed a “ropy” phenotype, while scanning electron microscopy (SEM) revealed strands of polysaccharide-linking neighboring cells. Beta-glucan biosynthesis was confirmed by agglutination tests carried out with Streptococcus pneumoniae type 37-specific antibodies, which specifically detect glucan-producing cells. Further analysis showed a ∼2-fold increase in viscosity in broth media for the beta-glucan-producing strain over 24 h compared to the control strain, which did not show any significant increase in viscosity. In addition, we analyzed the ability of beta-glucan-producing Lactobacillus paracasei NFBC 338 to survive both technological and gastrointestinal stresses. Heat stress assays revealed that production of the polysaccharide was associated with significantly increased protection during heat stress (60-fold), acid stress (20-fold), and simulated gastric juice stress (15-fold). Bile stress assays revealed a more modest but significant 5.5-fold increase in survival for the beta-glucan-producing strain compared to that of the control strain. These results suggest that production of a beta-glucan exopolysaccharide by strains destined for use as probiotics may afford them greater performance/protection during cultivation, processing, and ingestion. As such, expression of the gtf gene may prove to be a straightforward approach to improve strains that might otherwise prove sensitive in such applications. PMID:19933353

  11. Yield and Failure Behavior Investigated for Cross-Linked Phenolic Resins Using Molecular Dynamics

    NASA Technical Reports Server (NTRS)

    Monk, Joshua D.; Lawson, John W.

    2016-01-01

    Molecular dynamics simulations were conducted to fundamentally evaluate the yield and failure behavior of cross-linked phenolic resins at temperatures below the glass transition. Yield stress was investigated at various temperatures, strain rates, and degrees of cross-linking. The onset of non-linear behavior in the cross-linked phenolic structures was caused by localized irreversible molecular rearrangements through the rotation of methylene linkers followed by the formation or annihilation of neighboring hydrogen bonds. The yield stress results, with respect to temperature and strain rate, could be fit by existing models used to describe yield behavior of amorphous glasses. The degree of cross-linking only indirectly influences the maximum yield stress through its influence on glass transition temperature (Tg), however there is a strong relationship between the degree of cross-linking and the failure mechanism. Low cross-linked samples were able to separate through void formation, whereas the highly cross-linked structures exhibited bond scission.

  12. Enhanced Locomotor Activity Is Required to Exert Dietary Restriction-Dependent Increase of Stress Resistance in Drosophila.

    PubMed

    Ghimire, Saurav; Kim, Man Su

    2015-01-01

    Dietary restriction (DR) is known to be one of the most effective interventions to increase stress resistance, yet the mechanisms remain elusive. One of the most obvious DR-induced changes in phenotype is an increase in locomotor activity. Although it is conceptually perceivable that nutritional scarcity should prompt enhanced foraging behavior to garner additional dietary resources, the significance of enhanced movement activity has not been associated with the DR-dependent increase of stress resistance. In this study, we confirmed that flies raised on DR exhibited enhanced locomotive activity and increased stress resistance. Excision of fly wings minimized the DR-induced increase in locomotive activity, which resulted in attenuation of the DR-dependent increase of stress resistance. The possibility that wing clipping counteracts the DR by coercing flies to have more intake was ruled out since it did not induce any weight gain. Rather it was found that elimination of reactive oxygen species (ROS) that is enhanced by DR-induced upregulation of expression of antioxidant genes was significantly reduced by wing clipping. Collectively, our data suggests that DR increased stress resistance by increasing the locomotor activity, which upregulated expression of protective genes including, but not limited to, ROS scavenger system.

  13. Carbon monoxide improves neuronal differentiation and yield by increasing the functioning and number of mitochondria.

    PubMed

    Almeida, Ana S; Sonnewald, Ursula; Alves, Paula M; Vieira, Helena L A

    2016-08-01

    The process of cell differentiation goes hand-in-hand with metabolic adaptations, which are needed to provide energy and new metabolites. Carbon monoxide (CO) is an endogenous cytoprotective molecule able to inhibit cell death and improve mitochondrial metabolism. Neuronal differentiation processes were studied using the NT2 cell line, which is derived from human testicular embryonic teratocarcinoma and differentiates into post-mitotic neurons upon retinoic acid treatment. CO-releasing molecule A1 (CORM-A1) was used do deliver CO into cell culture. CO treatment improved NT2 neuronal differentiation and yield, since there were more neurons and the total cell number increased following the differentiation process. CO supplementation enhanced the mitochondrial population in post-mitotic neurons derived from NT2 cells, as indicated by an increase in mitochondrial DNA. CO treatment during neuronal differentiation increased the extent of the classical metabolic change that occurs during neuronal differentiation, from glycolytic to more oxidative metabolism, by decreasing the ratio of lactate production and glucose consumption. The expression of pyruvate and lactate dehydrogenases was higher, indicating an augmented oxidative metabolism. Moreover, these findings were corroborated by an increased percentage of (13) C incorporation from [U-(13) C]glucose into the tricarboxylic acid cycle metabolites malate and citrate, and also glutamate and aspartate in CO-treated cells. Finally, under low levels of oxygen (5%), which enhances glycolytic metabolism, some of the enhancing effects of CO on mitochondria were not observed. In conclusion, our data show that CO improves neuronal and mitochondrial yield by stimulation of tricarboxylic acid cycle activity, and thus oxidative metabolism of NT2 cells during the process of neuronal differentiation. The process of cell differentiation is coupled with metabolic adaptations. Carbon monoxide (CO) is an endogenous cytoprotective

  14. Historical gains in soybean (Glycine max Merr.) seed yield are driven by linear increases in light interception, energy conversion, and partitioning efficiencies.

    PubMed

    Koester, Robert P; Skoneczka, Jeffrey A; Cary, Troy R; Diers, Brian W; Ainsworth, Elizabeth A

    2014-07-01

    Soybean (Glycine max Merr.) is the world's most widely grown leguminous crop and an important source of protein and oil for food and feed. Soybean yields have increased substantially throughout the past century, with yield gains widely attributed to genetic advances and improved cultivars as well as advances in farming technology and practice. Yet, the physiological mechanisms underlying the historical improvements in soybean yield have not been studied rigorously. In this 2-year experiment, 24 soybean cultivars released between 1923 and 2007 were grown in field trials. Physiological improvements in the efficiencies by which soybean canopies intercepted light (εi), converted light energy into biomass (εc), and partitioned biomass into seed (εp) were examined. Seed yield increased by 26.5kg ha(-1) year(-1), and the increase in seed yield was driven by improvements in all three efficiencies. Although the time to canopy closure did not change in historical soybean cultivars, extended growing seasons and decreased lodging in more modern lines drove improvements in εi. Greater biomass production per unit of absorbed light resulted in improvements in εc. Over 84 years of breeding, soybean seed biomass increased at a rate greater than total aboveground biomass, resulting in an increase in εp. A better understanding of the physiological basis for yield gains will help to identify targets for soybean improvement in the future. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  15. Overexpression of rice serotonin N-acetyltransferase 1 in transgenic rice plants confers resistance to cadmium and senescence and increases grain yield.

    PubMed

    Lee, Kyungjin; Back, Kyoungwhan

    2017-04-01

    While ectopic overexpression of serotonin N-acetyltransferase (SNAT) in plants has been accomplished using animal SNAT genes, ectopic overexpression of plant SNAT genes in plants has not been investigated. Because the plant SNAT protein differs from that of animals in its subcellular localization and enzyme kinetics, its ectopic overexpression in plants would be expected to give outcomes distinct from those observed from overexpression of animal SNAT genes in transgenic plants. Consistent with our expectations, we found that transgenic rice plants overexpressing rice (Oryza sativa) SNAT1 (OsSNAT1) did not show enhanced seedling growth like that observed in ovine SNAT-overexpressing transgenic rice plants, although both types of plants exhibited increased melatonin levels. OsSNAT1-overexpressing rice plants did show significant resistance to cadmium and senescence stresses relative to wild-type controls. In contrast to tomato, melatonin synthesis in rice seedlings was not induced by selenium and OsSNAT1 transgenic rice plants did not show tolerance to selenium. T 2 homozygous OsSNAT1 transgenic rice plants exhibited increased grain yield due to increased panicle number per plant under paddy field conditions. These benefits conferred by ectopic overexpression of OsSNAT1 had not been observed in transgenic rice plants overexpressing ovine SNAT, suggesting that plant SNAT functions differently from animal SNAT in plants. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  16. Accelerated Growth Rate and Increased Drought Stress Resilience of the Model Grass Brachypodium distachyon Colonized by Bacillus subtilis B26.

    PubMed

    Gagné-Bourque, François; Mayer, Boris F; Charron, Jean-Benoit; Vali, Hojatollah; Bertrand, Annick; Jabaji, Suha

    2015-01-01

    Plant growth-promoting bacteria (PGB) induce positive effects in plants, for instance, increased growth and reduced abiotic stresses susceptibility. The mechanisms by which these bacteria impact the host plant are numerous, diverse and often specific. Here, we studied the agronomical, molecular and biochemical effects of the endophytic PGB Bacillus subtilis B26 on the full life cycle of Brachypodium distachyon Bd21, an established model species for functional genomics in cereal crops and temperate grasses. Inoculation of Brachypodium with B. subtilis strain B26 increased root and shoot weights, accelerated growth rate and seed yield as compared to control plants. B. subtilis strain B26 efficiently colonized the plant and was recovered from roots, stems and blades as well as seeds of Brachypodium, indicating that the bacterium is able to migrate, spread systemically inside the plant, establish itself in the aerial plant tissues and organs, and is vertically transmitted to seeds. The presence of B. subtilis strain B26 in the seed led to systemic colonization of the next generation of Brachypodium plants. Inoculated Brachypodium seedlings and mature plants exposed to acute and chronic drought stress minimized the phenotypic effect of drought compared to plants not harbouring the bacterium. Protection from the inhibitory effects of drought by the bacterium was linked to upregulation of the drought-response genes, DREB2B-like, DHN3-like and LEA-14-A-like and modulation of the DNA methylation genes, MET1B-like, CMT3-like and DRM2-like, that regulate the process. Additionally, total soluble sugars and starch contents increased in stressed inoculated plants, a biochemical indication of drought tolerance. In conclusion, we show a single inoculation of Brachypodium with a PGB affected the whole growth cycle of the plant, accelerating its growth rates, shortening its vegetative period, and alleviating drought stress effects. These effects are relevant to grasses and cereal

  17. Accelerated Growth Rate and Increased Drought Stress Resilience of the Model Grass Brachypodium distachyon Colonized by Bacillus subtilis B26

    PubMed Central

    Charron, Jean-Benoit; Vali, Hojatollah; Bertrand, Annick; Jabaji, Suha

    2015-01-01

    Plant growth-promoting bacteria (PGB) induce positive effects in plants, for instance, increased growth and reduced abiotic stresses susceptibility. The mechanisms by which these bacteria impact the host plant are numerous, diverse and often specific. Here, we studied the agronomical, molecular and biochemical effects of the endophytic PGB Bacillus subtilis B26 on the full life cycle of Brachypodium distachyon Bd21, an established model species for functional genomics in cereal crops and temperate grasses. Inoculation of Brachypodium with B. subtilis strain B26 increased root and shoot weights, accelerated growth rate and seed yield as compared to control plants. B. subtilis strain B26 efficiently colonized the plant and was recovered from roots, stems and blades as well as seeds of Brachypodium, indicating that the bacterium is able to migrate, spread systemically inside the plant, establish itself in the aerial plant tissues and organs, and is vertically transmitted to seeds. The presence of B. subtilis strain B26 in the seed led to systemic colonization of the next generation of Brachypodium plants. Inoculated Brachypodium seedlings and mature plants exposed to acute and chronic drought stress minimized the phenotypic effect of drought compared to plants not harbouring the bacterium. Protection from the inhibitory effects of drought by the bacterium was linked to upregulation of the drought-response genes, DREB2B-like, DHN3-like and LEA-14-A-like and modulation of the DNA methylation genes, MET1B-like, CMT3-like and DRM2-like, that regulate the process. Additionally, total soluble sugars and starch contents increased in stressed inoculated plants, a biochemical indication of drought tolerance. In conclusion, we show a single inoculation of Brachypodium with a PGB affected the whole growth cycle of the plant, accelerating its growth rates, shortening its vegetative period, and alleviating drought stress effects. These effects are relevant to grasses and cereal

  18. The association between rotating shift work and increased occupational stress in nurses.

    PubMed

    Lin, Pei-Chen; Chen, Chung-Hey; Pan, Shung-Mei; Chen, Yao-Mei; Pan, Chih-Hong; Hung, Hsin-Chia; Wu, Ming-Tsang

    2015-01-01

    The aim of this study was to investigate whether rotating shift work increases occupational stress in nurses. This study measured shift work scheduling and occupational stress by using the Effort-Reward Imbalance model with self-reported questionnaires in a sample of 654 female nurses. Overcommitment risk was higher in nurses who worked rotating shifts than in those who worked day/non-night shifts (OR, 2.16; 95% CI, 1.03-4.66). However, an effort/reward imbalance was not directly associated with work schedules (OR, 1.88; 95% CI, 0.87-4.35). Among nurses working rotation rotating shifts, those who had 2 days off after their most recent night shifts showed an alleviated risk of overcommitment (OR, 0.52; 95% CI, 0.32-0.82), but those who had worked for at least one series of 7 consecutive work days per month had an increased risk of effort/reward imbalance (OR, 2.75; 95% CI, 1.69-4.48). Additionally, those who had little or no participation in planning working hours and shift scheduling and worked overtime at least three times per week during the preceding 2 months tended to have high stress. The nurses who worked rotating shifts tended to experience work-related stress, but their stress levels improved if they had at least 2 days off after their most recent night shift and if they were not scheduled to work 7 consecutive days. These empirical data can be used to optimize work schedules for nurses to alleviate work stress.

  19. Hormonal and metabolic regulation of source-sink relations under salinity and drought: from plant survival to crop yield stability.

    PubMed

    Albacete, Alfonso A; Martínez-Andújar, Cristina; Pérez-Alfocea, Francisco

    2014-01-01

    Securing food production for the growing population will require closing the gap between potential crop productivity under optimal conditions and the yield captured by farmers under a changing environment, which is termed agronomical stability. Drought and salinity are major environmental factors contributing to the yield gap ultimately by inducing premature senescence in the photosynthetic source tissues of the plant and by reducing the number and growth of the harvestable sink organs by affecting the transport and use of assimilates between and within them. However, the changes in source-sink relations induced by stress also include adaptive changes in the reallocation of photoassimilates that influence crop productivity, ranging from plant survival to yield stability. While the massive utilization of -omic technologies in model plants is discovering hundreds of genes with potential impacts in alleviating short-term applied drought and salinity stress (usually measured as plant survival), only in relatively few cases has an effect on crop yield stability been proven. However, achieving the former does not necessarily imply the latter. Plant survival only requires water status conservation and delayed leaf senescence (thus maintaining source activity) that is usually accompanied by growth inhibition. However, yield stability will additionally require the maintenance or increase in sink activity in the reproductive structures, thus contributing to the transport of assimilates from the source leaves and to delayed stress-induced leaf senescence. This review emphasizes the role of several metabolic and hormonal factors influencing not only the source strength, but especially the sink activity and their inter-relations, and their potential to improve yield stability under drought and salinity stresses. © 2013.

  20. Cacao Cultivation under Diverse Shade Tree Cover Allows High Carbon Storage and Sequestration without Yield Losses

    PubMed Central

    Abou Rajab, Yasmin; Leuschner, Christoph; Barus, Henry; Tjoa, Aiyen; Hertel, Dietrich

    2016-01-01

    One of the main drivers of tropical forest loss is their conversion to oil palm, soy or cacao plantations with low biodiversity and greatly reduced carbon storage. Southeast Asian cacao plantations are often established under shade tree cover, but are later converted to non-shaded monocultures to avoid resource competition. We compared three co-occurring cacao cultivation systems (3 replicate stands each) with different shade intensity (non-shaded monoculture, cacao with the legume Gliricidia sepium shade trees, and cacao with several shade tree species) in Sulawesi (Indonesia) with respect to above- and belowground biomass and productivity, and cacao bean yield. Total biomass C stocks (above- and belowground) increased fivefold from the monoculture to the multi-shade tree system (from 11 to 57 Mg ha-1), total net primary production rose twofold (from 9 to 18 Mg C ha-1 yr-1). This increase was associated with a 6fold increase in aboveground biomass, but only a 3.5fold increase in root biomass, indicating a clear shift in C allocation to aboveground tree organs with increasing shade for both cacao and shade trees. Despite a canopy cover increase from 50 to 93%, cacao bean yield remained invariant across the systems (variation: 1.1–1.2 Mg C ha-1 yr-1). The monocultures had a twice as rapid leaf turnover suggesting that shading reduces the exposure of cacao to atmospheric drought, probably resulting in greater leaf longevity. Thus, contrary to general belief, cacao bean yield does not necessarily decrease under shading which seems to reduce physical stress. If planned properly, cacao plantations under a shade tree cover allow combining high yield with benefits for carbon sequestration and storage, production system stability under stress, and higher levels of animal and plant diversity. PMID:26927428

  1. Cacao Cultivation under Diverse Shade Tree Cover Allows High Carbon Storage and Sequestration without Yield Losses.

    PubMed

    Abou Rajab, Yasmin; Leuschner, Christoph; Barus, Henry; Tjoa, Aiyen; Hertel, Dietrich

    2016-01-01

    One of the main drivers of tropical forest loss is their conversion to oil palm, soy or cacao plantations with low biodiversity and greatly reduced carbon storage. Southeast Asian cacao plantations are often established under shade tree cover, but are later converted to non-shaded monocultures to avoid resource competition. We compared three co-occurring cacao cultivation systems (3 replicate stands each) with different shade intensity (non-shaded monoculture, cacao with the legume Gliricidia sepium shade trees, and cacao with several shade tree species) in Sulawesi (Indonesia) with respect to above- and belowground biomass and productivity, and cacao bean yield. Total biomass C stocks (above- and belowground) increased fivefold from the monoculture to the multi-shade tree system (from 11 to 57 Mg ha-1), total net primary production rose twofold (from 9 to 18 Mg C ha-1 yr-1). This increase was associated with a 6fold increase in aboveground biomass, but only a 3.5fold increase in root biomass, indicating a clear shift in C allocation to aboveground tree organs with increasing shade for both cacao and shade trees. Despite a canopy cover increase from 50 to 93%, cacao bean yield remained invariant across the systems (variation: 1.1-1.2 Mg C ha-1 yr-1). The monocultures had a twice as rapid leaf turnover suggesting that shading reduces the exposure of cacao to atmospheric drought, probably resulting in greater leaf longevity. Thus, contrary to general belief, cacao bean yield does not necessarily decrease under shading which seems to reduce physical stress. If planned properly, cacao plantations under a shade tree cover allow combining high yield with benefits for carbon sequestration and storage, production system stability under stress, and higher levels of animal and plant diversity.

  2. Increased intracellular proteolysis reduces disease severity in an ER stress-associated dwarfism.

    PubMed

    Mullan, Lorna A; Mularczyk, Ewa J; Kung, Louise H; Forouhan, Mitra; Wragg, Jordan Ma; Goodacre, Royston; Bateman, John F; Swanton, Eileithyia; Briggs, Michael D; Boot-Handford, Raymond P

    2017-10-02

    The short-limbed dwarfism metaphyseal chondrodysplasia type Schmid (MCDS) is linked to mutations in type X collagen, which increase ER stress by inducing misfolding of the mutant protein and subsequently disrupting hypertrophic chondrocyte differentiation. Here, we show that carbamazepine (CBZ), an autophagy-stimulating drug that is clinically approved for the treatment of seizures and bipolar disease, reduced the ER stress induced by 4 different MCDS-causing mutant forms of collagen X in human cell culture. Depending on the nature of the mutation, CBZ application stimulated proteolysis of misfolded collagen X by either autophagy or proteasomal degradation, thereby reducing intracellular accumulation of mutant collagen. In MCDS mice expressing the Col10a1.pN617K mutation, CBZ reduced the MCDS-associated expansion of the growth plate hypertrophic zone, attenuated enhanced expression of ER stress markers such as Bip and Atf4, increased bone growth, and reduced skeletal dysplasia. CBZ produced these beneficial effects by reducing the MCDS-associated abnormalities in hypertrophic chondrocyte differentiation. Stimulation of intracellular proteolysis using CBZ treatment may therefore be a clinically viable way of treating the ER stress-associated dwarfism MCDS.

  3. Apelin-APJ system is responsible for stress-induced increase in atrial natriuretic peptide expression in rat heart.

    PubMed

    Izgut-Uysal, Vecihe Nimet; Acar, Nuray; Birsen, Ilknur; Ozcan, Filiz; Ozbey, Ozlem; Soylu, Hakan; Avci, Sema; Tepekoy, Filiz; Akkoyunlu, Gokhan; Yucel, Gultekin; Ustunel, Ismail

    2018-04-01

    The cardiovascular system is a primary target of stress and stress is the most important etiologic factor in cardiovascular diseases. Stressors increase expressions of atrial natriuretic peptide (ANP) and apelin in cardiac tissue. The aim of the present study was to investigate whether stress-induced apelin has an effect on the expression of ANP in the right atrium of rat heart. The rats were divided into the control, stress and F13A+stress groups. In the stress and F13A+stress groups, the rats were subjected to water immersion and restraint stress (WIRS) for 6h. In the F13A+stress group, apelin receptor antagonist F13A, was injected intravenously immediately before application of WIRS. The plasma samples were obtained for the measurement of corticosterone and atrial natriuretic peptide. The atrial samples were used for immunohistochemistry and western blot analysis. F13A administration prevented the rise of plasma corticosterone and ANP levels induced by WIRS. While WIRS application increased the expressions of apelin, HIF-1α and ANP in atrial tissue, while F13A prevented the stress-induced increase in the expression of HIF-1α and ANP. Stress-induced apelin induces ANP expression in atrial tissue and may play a role in cardiovascular homeostasis by increasing ANP expression under WIRS conditions. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Exposure of rat hippocampal astrocytes to Ziram increases oxidative stress.

    PubMed

    Matei, Ann-Marie; Trombetta, Louis D

    2016-04-01

    Pesticides have been shown in several studies to be the leading candidates of environmental toxins and may contribute to the pathogenesis of several neurodegenerative diseases. Ziram (zinc-bis(dimethyldithiocarbamate)) is an agricultural dithiocarbamate fungicide that is used to treat a variety of plant diseases. In spite of their generally acknowledged low toxicity, dithiocarbamates are known to cause a wide range of neurobehavioral effects as well as neuropathological changes in the brain. Astrocytes play a key role in normal brain physiology and in the pathology of the nervous system. This investigation studied the effects of 1.0 µM Ziram on rat hippocampal astrocytes. The thiobarbituric acid reactive substance assay performed showed a significant increase in malondialdehyde, a product of lipid peroxidation, in the Ziram-treated cells. Biochemical analysis also revealed a significant increase in the induction of 70 kDa heat shock and heme oxygenase 1 stress proteins. In addition, an increase of glutathione peroxidase (GPx) and a significant increase in oxidized glutathione (GSSG) were observed in the Ziram-treated cells. The ratio GSH to GSSG calculated from the treated cells was also decreased. Light and transmission electron microscopy supported the biochemical findings in Ziram-treated astrocytes. This data suggest that the cytotoxic effects observed with Ziram treatments may be related to the increase of oxidative stress. © The Author(s) 2013.

  5. Targeting carbon for crop yield and drought resilience.

    PubMed

    Griffiths, Cara A; Paul, Matthew J

    2017-11-01

    Current methods of crop improvement are not keeping pace with projected increases in population growth. Breeding, focused around key traits of stem height and disease resistance, delivered the step-change yield improvements of the green revolution of the 1960s. However, subsequently, yield increases through conventional breeding have been below the projected requirement of 2.4% per year required by 2050. Genetic modification (GM) mainly for herbicide tolerance and insect resistance has been transformational, akin to a second green revolution, although GM has yet to make major inroads into intrinsic yield processes themselves. Drought imposes the major restriction on crop yields globally but, as yet, has not benefited substantially from genetic improvement and still presents a major challenge to agriculture. Much still has to be learnt about the complex process of how drought limits yield and what should be targeted. Mechanisms of drought adaptation from the natural environment cannot be taken into crops without significant modification for the agricultural environment because mechanisms of drought tolerance are often in contrast with mechanisms of high productivity required in agriculture. However, through convergence of fundamental and translational science, it would appear that a mechanism of sucrose allocation in crops can be modified for both productivity and resilience to drought and other stresses. Recent publications show how this mechanism can be targeted by GM, natural variation and a new chemical approach. Here, with an emphasis on drought, we highlight how understanding fundamental science about how crops grow, develop and what limits their growth and yield can be combined with targeted genetic selection and pioneering chemical intervention technology for transformational yield improvements. © 2017 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. © 2017 The Authors

  6. Translating knowledge about abiotic stress tolerance to breeding programmes.

    PubMed

    Gilliham, Matthew; Able, Jason A; Roy, Stuart J

    2017-06-01

    Plant breeding and improvements in agronomic practice are making a consistent contribution to increasing global crop production year upon year. However, the rate of yield improvement currently lags behind the targets set to produce enough food to meet the demands of the predicted global population in 2050. Furthermore, crops that are exposed to harmful abiotic environmental factors (abiotic stresses, e.g. water limitation, salinity, extreme temperature) are prone to reduced yields. Here, we briefly describe the processes undertaken in conventional breeding programmes, which are usually designed to improve yields in near-optimal conditions rather than specifically breeding for improved crop yield stability under stressed conditions. While there is extensive fundamental research activity that examines mechanisms of plant stress tolerance, there are few examples that apply this research to improving commercial crop yields. There are notable exceptions, and we highlight some of these to demonstrate the magnitude of yield gains that could be made by translating agronomic, phenological and genetic solutions focused on improving or mitigating the effect of abiotic stress in the field; in particular, we focus on improvements in crop water-use efficiency and salinity tolerance. We speculate upon the reasons for the disconnect between research and research translation. We conclude that to realise untapped rapid gains towards food security targets new funding structures need to be embraced. Such funding needs to serve both the core and collaborative activities of the fundamental, pre-breeding and breeding research communities in order to expedite the translation of innovative research into the fields of primary producers. © 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd.

  7. The metabolic costs of improving ethanol yield by reducing glycerol formation capacity under anaerobic conditions in Saccharomyces cerevisiae.

    PubMed

    Pagliardini, Julien; Hubmann, Georg; Alfenore, Sandrine; Nevoigt, Elke; Bideaux, Carine; Guillouet, Stephane E

    2013-03-28

    Finely regulating the carbon flux through the glycerol pathway by regulating the expression of the rate controlling enzyme, glycerol-3-phosphate dehydrogenase (GPDH), has been a promising approach to redirect carbon from glycerol to ethanol and thereby increasing the ethanol yield in ethanol production. Here, strains engineered in the promoter of GPD1 and deleted in GPD2 were used to investigate the possibility of reducing glycerol production of Saccharomyces cerevisiae without jeopardising its ability to cope with process stress during ethanol production. For this purpose, the mutant strains TEFmut7 and TEFmut2 with different GPD1 residual expression were studied in Very High Ethanol Performance (VHEP) fed-batch process under anaerobic conditions. Both strains showed a drastic reduction of the glycerol yield by 44 and 61% while the ethanol yield improved by 2 and 7% respectively. TEFmut2 strain showing the highest ethanol yield was accompanied by a 28% reduction of the biomass yield. The modulation of the glycerol formation led to profound redox and energetic changes resulting in a reduction of the ATP yield (YATP) and a modulation of the production of organic acids (acetate, pyruvate and succinate). Those metabolic rearrangements resulted in a loss of ethanol and stress tolerance of the mutants, contrarily to what was previously observed under aerobiosis. This work demonstrates the potential of fine-tuned pathway engineering, particularly when a compromise has to be found between high product yield on one hand and acceptable growth, productivity and stress resistance on the other hand. Previous study showed that, contrarily to anaerobiosis, the resulting gain in ethanol yield was accompanied with no loss of ethanol tolerance under aerobiosis. Moreover those mutants were still able to produce up to 90 gl-1 ethanol in an anaerobic SSF process. Fine tuning metabolic strategy may then open encouraging possibilities for further developing robust strains with improved

  8. Effects of hot, humid weather on milk temperature, dry matter intake, and milk yield of lactating dairy cows.

    PubMed

    West, J W; Mullinix, B G; Bernard, J K

    2003-01-01

    Lactating cows were exposed to moderate and hot, humid weather to determine the effect of increasing ambient temperature, relative humidity, or temperature-humidity index (THI) on intake, milk yield, and milk temperature. Minimum and maximum temperatures averaged 17.9 and 29.5 degrees C (cool period) and 22.5 and 34.4 degrees C (hot period), and minimum and maximum THI averaged 63.8 and 76.6 (cool period) and 72.1 and 83.6 (hot period). Environmental conditions had minor effects on intake and milk yield during the cool period. During the hot period, the THI 2 d earlier and mean air temperature 2 d earlier had the greatest impact on milk yield and DMI, respectively. Both breeds maintained milk temperature within normal ranges during the cool period, but Holstein and Jersey p.m. milk temperatures averaged 39.6 and 39.2 degrees C during the hot period. Current day mean air temperature during the hot period had the greatest impact on cow p.m. milk temperature, and minimum air temperature had the greatest influence on a.m. milk temperature. Dry matter intake and milk yield declined linearly with respective increases in air temperature or THI during the hot period and milk temperature increased linearly with increasing air temperature. Dry matter intake and milk yield both exhibited a curvilinear relationship with milk temperature. Environmental modifications should target the effects of high temperatures on cow body temperature and should modify the environment at critical times during the day when cows are stressed, including morning hours when ambient temperatures are typically cooler and cows are not assumed to be stressed.

  9. Hindlimb unloading increases oxidative stress and disrupts antioxidant capacity in skeletal muscle

    NASA Technical Reports Server (NTRS)

    Lawler, John M.; Song, Wook; Demaree, Scott R.; Bloomfield, S. A. (Principal Investigator)

    2003-01-01

    Skeletal muscle disuse with space-flight and ground-based models (e.g., hindlimb unloading) results in dramatic skeletal muscle atrophy and weakness. Pathological conditions that cause muscle wasting (i.e., heart failure, muscular dystrophy, sepsis, COPD, cancer) are characterized by elevated "oxidative stress," where antioxidant defenses are overwhelmed by oxidant production. However, the existence, cellular mechanisms, and ramifications of oxidative stress in skeletal muscle subjected to hindlimb unloading are poorly understood. Thus we examined the effects of hindlimb unloading on hindlimb muscle antioxidant enzymes (e.g., superoxide dismutase, catalase, glutathione peroxidase), nonenzymatic antioxidant scavenging capacity (ASC), total hydroperoxides, and dichlorohydrofluorescein diacetate (DCFH-DA) oxidation, a direct indicator of oxidative stress. Twelve 6 month old Sprague Dawley rats were divided into two groups: 28 d of hindlimb unloading (n = 6) and controls (n = 6). Hindlimb unloading resulted in a small decrease in Mn-superoxide dismutase activity (10.1%) in the soleus muscle, while Cu,Zn-superoxide dismutase increased 71.2%. In contrast, catalase and glutathione peroxidase, antioxidant enzymes that remove hydroperoxides, were significantly reduced in the soleus with hindlimb unloading by 54.5 and 16.1%, respectively. Hindlimb unloading also significantly reduced ASC. Hindlimb unloading increased soleus lipid hydroperoxide levels by 21.6% and hindlimb muscle DCFH-DA oxidation by 162.1%. These results indicate that hindlimb unloading results in a disruption of antioxidant status, elevation of hydroperoxides, and an increase in oxidative stress.

  10. Increased feeding frequency increased milk fat yield and may reduce the severity of subacute ruminal acidosis in higher-risk cows.

    PubMed

    Macmillan, K; Gao, X; Oba, M

    2017-02-01

    The objectives of this study were to determine whether feeding behavior is different between cows at higher or lower risk for subacute ruminal acidosis (SARA) and whether increasing feeding frequency could be used to reduce the severity of SARA in higher-risk cows. In preliminary studies, 16 ruminally cannulated lactating cows were fed high-grain diets once per day to increase the risk of SARA. After a 17-d diet adaptation, ruminal pH was measured every 30 s over 24 h. Cows were classified as higher-risk (n = 7) or lower-risk (n = 9) for SARA based on an acidosis index (area of pH <5.8/dry matter intake). Feeding behavior was recorded every 5 min over the same 24 h. The 24-h observation period was analyzed in 3 periods of 8 h after feeding. Although there was no significant difference in overall dry matter intake, higher-risk cows spent more time eating in the first 8-h period after feeding than lower-risk cows (186 vs. 153 min) and less time eating in the third 8-h period (19 vs. 43 min). In the primary experiment, 8 ruminally cannulated lactating cows were fed a high-grain diet once per day (1×; 0800 h) or 3 times per day (3×; 0800, 1500, and 2000 h) in a crossover design with 21-d periods (16 d of treatment adaptation and 5 d of data collection). Rumen pH and feeding behavior were measured over 72 h. Behavior data were summarized separately for the 3 periods (0800 to 1500, 1500 to 2200, and 2200 to 0800 h). Four cows were categorized as higher-risk and 4 as lower-risk, based on their acidosis index. The 3× feeding reduced eating time between 0800 and 1500 h (99 vs. 145 min) and increased eating time between 2200 and 0800 h (76 vs. 43 min) for all cows, regardless of category, compared with 1× feeding. For higher-risk cows, 3× feeding reduced the area below pH 5.8 (51 vs. 98 pH × min/d), but it did not affect rumen pH for the lower-risk cows. Milk yield was not different between groups, but 3× feeding increased milk fat yield (1.22 vs. 1.08 kg/d) for all

  11. Holographic lens spectrum splitting photovoltaic system for increased diffuse collection and annual energy yield

    NASA Astrophysics Data System (ADS)

    Vorndran, Shelby D.; Wu, Yuechen; Ayala, Silvana; Kostuk, Raymond K.

    2015-09-01

    Concentrating and spectrum splitting photovoltaic (PV) modules have a limited acceptance angle and thus suffer from optical loss under off-axis illumination. This loss manifests itself as a substantial reduction in energy yield in locations where a significant portion of insulation is diffuse. In this work, a spectrum splitting PV system is designed to efficiently collect and convert light in a range of illumination conditions. The system uses a holographic lens to concentrate shortwavelength light onto a smaller, more expensive indium gallium phosphide (InGaP) PV cell. The high efficiency PV cell near the axis is surrounded with silicon (Si), a less expensive material that collects a broader portion of the solar spectrum. Under direct illumination, the device achieves increased conversion efficiency from spectrum splitting. Under diffuse illumination, the device collects light with efficiency comparable to a flat-panel Si module. Design of the holographic lens is discussed. Optical efficiency and power output of the module under a range of illumination conditions from direct to diffuse are simulated with non-sequential raytracing software. Using direct and diffuse Typical Metrological Year (TMY3) irradiance measurements, annual energy yield of the module is calculated for several installation sites. Energy yield of the spectrum splitting module is compared to that of a full flat-panel Si reference module.

  12. Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre.

    PubMed

    Zhao, X; Moates, G K; Elliston, A; Wilson, D R; Coleman, M J; Waldron, K W

    2015-10-01

    This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210°C, 10 min) and then subjected to simultaneous saccharification and fermentation (SSF) using Cellic® CTec 2 (20 U or 0.87 FPU g(-1) substrate) cellulase plus β-glucosidase (2 U g(-1) substrate) and a yeast inoculum of 10% (v/v or 8.0×10(7) cells mL(-1)). At a substrate concentration of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concentration of 20% (w/v) by metabolising fermentation inhibitors. Copyright © 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.

  13. Influence of yield surface curvature on the macroscopic yielding and ductile failure of isotropic porous plastic materials

    NASA Astrophysics Data System (ADS)

    Dæhli, Lars Edvard Bryhni; Morin, David; Børvik, Tore; Hopperstad, Odd Sture

    2017-10-01

    Numerical unit cell models of an approximative representative volume element for a porous ductile solid are utilized to investigate differences in the mechanical response between a quadratic and a non-quadratic matrix yield surface. A Hershey equivalent stress measure with two distinct values of the yield surface exponent is employed as the matrix description. Results from the unit cell calculations are further used to calibrate a heuristic extension of the Gurson model which incorporates effects of the third deviatoric stress invariant. An assessment of the porous plasticity model reveals its ability to describe the unit cell response to some extent, however underestimating the effect of the Lode parameter for the lower triaxiality ratios imposed in this study when compared to unit cell simulations. Ductile failure predictions by means of finite element simulations using a unit cell model that resembles an imperfection band are then conducted to examine how the non-quadratic matrix yield surface influences the failure strain as compared to the quadratic matrix yield surface. Further, strain localization predictions based on bifurcation analyses and imperfection band analyses are undertaken using the calibrated porous plasticity model. These simulations are then compared to the unit cell calculations in order to elucidate the differences between the various modelling strategies. The current study reveals that strain localization analyses using an imperfection band model and a spatially discretized unit cell are in reasonable agreement, while the bifurcation analyses predict higher strain levels at localization. Imperfection band analyses are finally used to calculate failure loci for the quadratic and the non-quadratic matrix yield surface under a wide range of loading conditions. The underlying matrix yield surface is demonstrated to have a pronounced influence on the onset of strain localization.

  14. Using satellite data to identify the causes of and potential solutions for yield gaps in India’s Wheat Belt

    NASA Astrophysics Data System (ADS)

    Jain, M.; Singh, Balwinder; Srivastava, A. A. K.; Malik, R. K.; McDonald, A. J.; Lobell, D. B.

    2017-09-01

    Food security will be increasingly challenged by climate change, natural resource degradation, and population growth. Wheat yields, in particular, have already stagnated in many regions and will be further affected by warming temperatures. Despite these challenges, wheat yields can be increased by improving management practices in regions with existing yield gaps. To identify the magnitude and causes of current yield gaps in India, one of the largest wheat producers globally, we produced 30 meter resolution yield maps from 2001 to 2015 across the Indo-Gangetic Plains (IGP), the nation’s main wheat belt. Yield maps were derived using a new method that translates satellite vegetation indices to yield estimates using crop model simulations, bypassing the need for ground calibration data. This is one of the first attempts to apply this method to a smallholder agriculture system, where ground calibration data are rarely available. We find that yields can be increased by 11% on average and up to 32% in the eastern IGP by improving management to current best practices within a given district. Additionally, if current best practices from the highest-yielding state of Punjab are implemented in the eastern IGP, yields could increase by almost 110%. Considering the factors that most influence yields, later sow dates and warmer temperatures are most associated with low yields across the IGP. This suggests that strategies to reduce the negative effects of heat stress, like earlier sowing and planting heat-tolerant wheat varieties, are critical to increasing wheat yields in this globally-important agricultural region.

  15. An assessment of irrigation needs and crop yield for the United States under potential climate changes

    USGS Publications Warehouse

    Brumbelow, Kelly; Georgakakos, Aris P.

    2000-01-01

    Past assessments of climate change on U.S. agriculture have mostly focused on changes in crop yield. Few studies have included the entire conterminous U.S., and few studies have assessed changing irrigation requirements. None have included the effects of changing soil moisture characteristics as determined by changing climatic forcing. This study assesses changes in irrigation requirements and crop yields for five crops in the areas of the U.S. where they have traditionally been grown. Physiologically-based crop models are used to incorporate inputs of climate, soils, agricultural management, and drought stress tolerance. Soil moisture values from a macroscale hydrologic model run under a future climate scenario are used to initialize soil moisture content at the beginning of each growing season. Historical crop yield data is used to calibrate model parameters and determine locally acceptable drought stress as a management parameter. Changes in irrigation demand and crop yield are assessed for both means and extremes by comparing results for atmospheric forcing close to the present climate with those for a future climate scenario. Assessments using the Canadian Center for Climate Modeling and Analysis General Circulation Model (CGCM1) indicate greater irrigation demands in the southern U.S. and decreased irrigation demands in the northern and western U.S. Crop yields typically increase except for winter wheat in the southern U.S. and corn. Variability in both irrigation demands and crop yields increases in most cases. Assessment results for the CGCM1 climate scenario are compared to those for the Hadley Centre for Climate Prediction and Research GCM (HadCM2) scenario for southwestern Georgia. The comparison shows significant differences in irrigation and yield trends, both in magnitude and direction. The differences reflect the high forecast uncertainty of current GCMs. Nonetheless, both GCMs indicate higher variability in future climatic forcing and, consequently

  16. The QTL GNP1 Encodes GA20ox1, Which Increases Grain Number and Yield by Increasing Cytokinin Activity in Rice Panicle Meristems.

    PubMed

    Wu, Yuan; Wang, Yun; Mi, Xue-Fei; Shan, Jun-Xiang; Li, Xin-Min; Xu, Jian-Long; Lin, Hong-Xuan

    2016-10-01

    Cytokinins and gibberellins (GAs) play antagonistic roles in regulating reproductive meristem activity. Cytokinins have positive effects on meristem activity and maintenance. During inflorescence meristem development, cytokinin biosynthesis is activated via a KNOX-mediated pathway. Increased cytokinin activity leads to higher grain number, whereas GAs negatively affect meristem activity. The GA biosynthesis genes GA20oxs are negatively regulated by KNOX proteins. KNOX proteins function as modulators, balancing cytokinin and GA activity in the meristem. However, little is known about the crosstalk among cytokinin and GA regulators together with KNOX proteins and how KNOX-mediated dynamic balancing of hormonal activity functions. Through map-based cloning of QTLs, we cloned a GA biosynthesis gene, Grain Number per Panicle1 (GNP1), which encodes rice GA20ox1. The grain number and yield of NIL-GNP1TQ were significantly higher than those of isogenic control (Lemont). Sequence variations in its promoter region increased the levels of GNP1 transcripts, which were enriched in the apical regions of inflorescence meristems in NIL-GNP1TQ. We propose that cytokinin activity increased due to a KNOX-mediated transcriptional feedback loop resulting from the higher GNP1 transcript levels, in turn leading to increased expression of the GA catabolism genes GA2oxs and reduced GA1 and GA3 accumulation. This rebalancing process increased cytokinin activity, thereby increasing grain number and grain yield in rice. These findings uncover important, novel roles of GAs in rice florescence meristem development and provide new insights into the crosstalk between cytokinin and GA underlying development process.

  17. Intake of green tea inhibited increase of salivary chromogranin A after mental task stress loads

    PubMed Central

    2014-01-01

    Background Green tea has become renowned for its health benefits. In this study, we investigated the anti-stress effect of two kinds of green tea against a mental stress task load. Methods Warm water, ordinary green tea (Sagara), and shaded white tea, which contains more amino acid components than Sagara, were used as test samples in a randomized cross-over design study. Eighteen students (nine male and nine female) participated in three experimental trials on different days at intervals of seven days. Saliva was collected before beverage intake and after performing the mental stress load tasks. Concentration of chromogranin A (CgA) in the saliva was used as an index of autonomic nervous system activity. Results CgA level increased after the mental tasks, but intake of green tea inhibited this increase; the anti-stress effect was even greater after consumption of shaded white tea. Intake of shaded white tea also lowered Total Mood Disturbance (TMD) score on the Profile of Mood States (POMS); subjects in this condition tended to perform more calculations in the arithmetic task than those in the warm water treatment condition. Conclusions Salivary CgA concentration levels increased after mental stress load tasks, but ingestion of green tea inhibited this increase. This anti-stress effect was larger after the consumption of shaded white tea than after Sagara. Shaded white tea intake also lowered TMD score (POMS) and tended to improve performance on an arithmetic task compared to warm water, suggesting that shaded white tea might also improve mood during and after mental stress load. PMID:25034805

  18. Cowpea (Vigna unguiculata L. Walp.) Metabolomics: Osmoprotection as a Physiological Strategy for Drought Stress Resistance and Improved Yield.

    PubMed

    Goufo, Piebiep; Moutinho-Pereira, José M; Jorge, Tiago F; Correia, Carlos M; Oliveira, Manuela R; Rosa, Eduardo A S; António, Carla; Trindade, Henrique

    2017-01-01

    Plants usually tolerate drought by producing organic solutes, which can either act as compatible osmolytes for maintaining turgor, or radical scavengers for protecting cellular functions. However, these two properties of organic solutes are often indistinguishable during stress progression. This study looked at individualizing properties of osmotic adjustment vs. osmoprotection in plants, using cowpea as the model species. Two cultivars were grown in well-watered soil, drought conditions, or drought followed by rewatering through fruit formation. Osmoadaptation was investigated in leaves and roots using photosynthetic traits, water homoeostasis, inorganic ions, and primary and secondary metabolites. Multifactorial analyses indicated allocation of high quantities of amino acids, sugars, and proanthocyanidins into roots, presumably linked to their role in growth and initial stress perception. Physiological and metabolic changes developed in parallel and drought/recovery responses showed a progressive acclimation of the cowpea plant to stress. Of the 88 metabolites studied, proline, galactinol, and a quercetin derivative responded the most to drought as highlighted by multivariate analyses, and their correlations with yield indicated beneficial effects. These metabolites accumulated differently in roots, but similarly in leaves, suggesting a more conservative strategy to cope with drought in the aerial parts. Changes in these compounds roughly reflected energy investment in protective mechanisms, although the ability of plants to adjust osmotically through inorganic ions uptake could not be discounted.

  19. Cowpea (Vigna unguiculata L. Walp.) Metabolomics: Osmoprotection as a Physiological Strategy for Drought Stress Resistance and Improved Yield

    PubMed Central

    Goufo, Piebiep; Moutinho-Pereira, José M.; Jorge, Tiago F.; Correia, Carlos M.; Oliveira, Manuela R.; Rosa, Eduardo A. S.; António, Carla; Trindade, Henrique

    2017-01-01

    Plants usually tolerate drought by producing organic solutes, which can either act as compatible osmolytes for maintaining turgor, or radical scavengers for protecting cellular functions. However, these two properties of organic solutes are often indistinguishable during stress progression. This study looked at individualizing properties of osmotic adjustment vs. osmoprotection in plants, using cowpea as the model species. Two cultivars were grown in well-watered soil, drought conditions, or drought followed by rewatering through fruit formation. Osmoadaptation was investigated in leaves and roots using photosynthetic traits, water homoeostasis, inorganic ions, and primary and secondary metabolites. Multifactorial analyses indicated allocation of high quantities of amino acids, sugars, and proanthocyanidins into roots, presumably linked to their role in growth and initial stress perception. Physiological and metabolic changes developed in parallel and drought/recovery responses showed a progressive acclimation of the cowpea plant to stress. Of the 88 metabolites studied, proline, galactinol, and a quercetin derivative responded the most to drought as highlighted by multivariate analyses, and their correlations with yield indicated beneficial effects. These metabolites accumulated differently in roots, but similarly in leaves, suggesting a more conservative strategy to cope with drought in the aerial parts. Changes in these compounds roughly reflected energy investment in protective mechanisms, although the ability of plants to adjust osmotically through inorganic ions uptake could not be discounted. PMID:28473840

  20. A 100-Year Review: Stress physiology including heat stress.

    PubMed

    Collier, R J; Renquist, B J; Xiao, Y

    2017-12-01

    Stress is an external event or condition that places a strain on a biological system. The animal response to a stress involves the expenditure of energy to remove or reduce the impact of the stress. This increases maintenance requirements of the animal and results in loss of production. The biological response to stress is divided into acute and chronic phases, with the acute phase lasting hours to a few days and the chronic phase lasting several days to weeks. The acute response is driven by homeostatic regulators of the nervous and endocrine systems and the chronic phase by homeorhetic regulators of the endocrine system. Both responses involve alterations in energy balance and metabolism. Thermal environment affects all animals and therefore represents the largest single stressor in animal production. Other types of stressors include housing conditions, overcrowding, social rank, disease, and toxic compounds. "Acclimation" to a stress is a phenotypic response developed by the animal to an individual stressor within the environment. However, under natural conditions, it is rare for only one environmental variable to change over time. "Acclimatization" is the process by which an animal adapts to several stressors within its natural environment. Acclimation is a homeorhetic process that takes several weeks to occur and occurs via homeorhetic, not homeostatic, mechanisms. It is a phenotypic change that disappears when the stress is removed. When the stress is severe and not relieved by acclimatization or management changes, the animal is considered chronically stressed and is susceptible to increased incidence of disease and poor health. Milk yield and reproduction are extremely sensitive to stress because of the high energy and protein demands of lactation and the complexity of the reproductive process and multiple organs that are involved. Improvements in protection of animals against stress require improved education of producers to recognize stress and methods

  1. Genetic control of soybean seed oil: II. QTL and genes that increase oil concentration without decreasing protein or with increased seed yield.

    PubMed

    Eskandari, Mehrzad; Cober, Elroy R; Rajcan, Istvan

    2013-06-01

    Soybean [Glycine max (L.) Merrill] seed oil is the primary global source of edible oil and a major renewable and sustainable feedstock for biodiesel production. Therefore, increasing the relative oil concentration in soybean is desirable; however, that goal is complex due to the quantitative nature of the oil concentration trait and possible effects on major agronomic traits such as seed yield or protein concentration. The objectives of the present study were to study the relationship between seed oil concentration and important agronomic and seed quality traits, including seed yield, 100-seed weight, protein concentration, plant height, and days to maturity, and to identify oil quantitative trait loci (QTL) that are co-localized with the traits evaluated. A population of 203 F4:6 recombinant inbred lines, derived from a cross between moderately high oil soybean genotypes OAC Wallace and OAC Glencoe, was developed and grown across multiple environments in Ontario, Canada, in 2009 and 2010. Among the 11 QTL associated with seed oil concentration in the population, which were detected using either single-factor ANOVA or multiple QTL mapping methods, the number of QTL that were co-localized with other important traits QTL were six for protein concentration, four for seed yield, two for 100-seed weight, one for days to maturity, and one for plant height. The oil-beneficial allele of the QTL tagged by marker Sat_020 was positively associated with seed protein concentration. The oil favorable alleles of markers Satt001 and GmDGAT2B were positively correlated with seed yield. In addition, significant two-way epistatic interactions, where one of the interacting markers was solely associated with seed oil concentration, were identified for the selected traits in this study. The number of significant epistatic interactions was seven for yield, four for days to maturity, two for 100-seed weight, one for protein concentration, and one for plant height. The identified molecular

  2. Testing the responses of four wheat crop models to heat stress at anthesis and grain filling.

    PubMed

    Liu, Bing; Asseng, Senthold; Liu, Leilei; Tang, Liang; Cao, Weixing; Zhu, Yan

    2016-05-01

    Higher temperatures caused by future climate change will bring more frequent heat stress events and pose an increasing risk to global wheat production. Crop models have been widely used to simulate future crop productivity but are rarely tested with observed heat stress experimental datasets. Four wheat models (DSSAT-CERES-Wheat, DSSAT-Nwheat, APSIM-Wheat, and WheatGrow) were evaluated with 4 years of environment-controlled phytotron experimental datasets with two wheat cultivars under heat stress at anthesis and grain filling stages. Heat stress at anthesis reduced observed grain numbers per unit area and individual grain size, while heat stress during grain filling mainly decreased the size of the individual grains. The observed impact of heat stress on grain filling duration, total aboveground biomass, grain yield, and grain protein concentration (GPC) varied depending on cultivar and accumulated heat stress. For every unit increase of heat degree days (HDD, degree days over 30 °C), grain filling duration was reduced by 0.30-0.60%, total aboveground biomass was reduced by 0.37-0.43%, and grain yield was reduced by 1.0-1.6%, but GPC was increased by 0.50% for cv Yangmai16 and 0.80% for cv Xumai30. The tested crop simulation models could reproduce some of the observed reductions in grain filling duration, final total aboveground biomass, and grain yield, as well as the observed increase in GPC due to heat stress. Most of the crop models tended to reproduce heat stress impacts better during grain filling than at anthesis. Some of the tested models require improvements in the response to heat stress during grain filling, but all models need improvements in simulating heat stress effects on grain set during anthesis. The observed significant genetic variability in the response of wheat to heat stress needs to be considered through cultivar parameters in future simulation studies. © 2016 John Wiley & Sons Ltd.

  3. Different singularities in the functions of extended kinetic theory at the origin of the yield stress in granular flows

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Berzi, Diego; Vescovi, Dalila

    2015-01-15

    We use previous results from discrete element simulations of simple shear flows of rigid, identical spheres in the collisional regime to show that the volume fraction-dependence of the stresses is singular at the shear rigidity. Here, we identify the shear rigidity, which is a decreasing function of the interparticle friction, as the maximum volume fraction beyond which a random collisional assembly of grains cannot be sheared without developing force chains that span the entire domain. In the framework of extended kinetic theory, i.e., kinetic theory that accounts for the decreasing in the collisional dissipation due to the breaking of molecularmore » chaos at volume fractions larger than 0.49, we also show that the volume fraction-dependence of the correlation length (measure of the velocity correlation) is singular at random close packing, independent of the interparticle friction. The difference in the singularities ensures that the ratio of the shear stress to the pressure at shear rigidity is different from zero even in the case of frictionless spheres: we identify that with the yield stress ratio of granular materials, and we show that the theoretical predictions, once the different singularities are inserted into the functions of extended kinetic theory, are in excellent agreement with the results of numerical simulations.« less

  4. Increasing adult hippocampal neurogenesis in mice after exposure to unpredictable chronic mild stress may counteract some of the effects of stress.

    PubMed

    Culig, Luka; Surget, Alexandre; Bourdey, Marlene; Khemissi, Wahid; Le Guisquet, Anne-Marie; Vogel, Elise; Sahay, Amar; Hen, René; Belzung, Catherine

    2017-11-01

    Major depression is hypothesized to be associated with dysregulations of the hypothalamic-pituitary-adrenal (HPA) axis and impairments in adult hippocampal neurogenesis. Adult-born hippocampal neurons are required for several effects of antidepressants and increasing the rate of adult hippocampal neurogenesis (AHN) before exposure to chronic corticosterone is sufficient to protect against its harmful effects on behavior. However, it is an open question if increasing AHN after the onset of chronic stress exposure would be able to rescue behavioral deficits and which mechanisms might be involved in recovery. We investigated this question by using a 10-week unpredictable chronic mild stress (UCMS) model on a transgenic mouse line (iBax mice), in which the pro-apoptotic gene Bax can be inducibly ablated in neural stem cells following Tamoxifen injection, therefore enhancing the survival of newborn neurons in the adult brain. We did not observe any effect of our treatment in non-stress conditions, but we did find that increasing AHN after 2 weeks of UCMS is sufficient to counteract the effects of UCMS on certain behaviors (splash test and changes in coat state) and endocrine levels and thus to display some antidepressant-like effects. We observed that increasing AHN lowered the elevated basal corticosterone levels in mice exposed to UCMS. This was accompanied by a tamoxifen-induced reversal of the lack of stress-induced decrease in neuronal activation in the anteromedial division of the bed nucleus of the stria terminalis (BSTMA) after intrahippocampal dexamethasone infusion, pointing to a possible mechanism through which adult-born neurons might have exerted their effects. Our results contribute to the neurogenesis hypothesis of depression by suggesting that increasing AHN may be beneficial not just before, but also after exposure to stress by counteracting several of its effects, in part through regulating the HPA axis. Copyright © 2017 Elsevier Ltd. All rights

  5. Short-term stress experienced at time of immunization induces a long-lasting increase in immunologic memory.

    PubMed

    Dhabhar, Firdaus S; Viswanathan, Kavitha

    2005-09-01

    It would be extremely beneficial if one could harness natural, endogenous, health-promoting defense mechanisms to fight disease and restore health. The psychophysiological stress response is the most underappreciated of nature's survival mechanisms. We show that acute stress experienced before primary immunization induces a long-lasting increase in immunity. Compared with controls, mice restrained for 2.5 h before primary immunization with keyhole limpet hemocyanin (KLH) show a significantly enhanced immune response when reexposed to KLH 9 mo later. This immunoenhancement is mediated by an increase in numbers of memory and effector helper T cells in sentinel lymph nodes at the time of primary immunization. Further analyses show that the early stress-induced increase in T cell memory may stimulate the robust increase in infiltrating lymphocyte and macrophage numbers observed months later at a novel site of antigen reexposure. Enhanced leukocyte infiltration may be driven by increased levels of the type 1 cytokines, IL-2 and IFN-gamma, and TNF-alpha, observed at the site of antigen reexposure in animals that had been stressed at the time of primary immunization. In contrast, no differences were observed in type 2 cytokines, IL-4 or IL-5. Given the importance of inducing long-lasting increases in immunologic memory during vaccination, we suggest that the neuroendocrine stress response is nature's adjuvant that could be psychologically and/or pharmacologically manipulated to safely increase vaccine efficacy. These studies introduce the novel concept that a psychophysiological stress response is nature's fundamental survival mechanism that could be therapeutically harnessed to augment immune function during vaccination, wound healing, or infection.

  6. Drought genetics have varying influence on corn water stress under differing water availability

    USDA-ARS?s Scientific Manuscript database

    Irrigated corn (Zea mays L.) in the Great Plains will be increasingly grown under limited irrigation management and greater water stress. Hybrids with drought genetics may decrease the impacts of water stress on yield. The objective of this experiment was to evaluate the effect of drought genetics o...

  7. Early Childhood Stress and Child Age Predict Longitudinal Increases in Obesogenic Eating Among Low-Income Children.

    PubMed

    Miller, Alison L; Gearhardt, Ashley N; Retzloff, Lauren; Sturza, Julie; Kaciroti, Niko; Lumeng, Julie C

    2018-01-31

    To identify whether psychosocial stress exposure during early childhood predicts subsequent increased eating in the absence of hunger (EAH), emotional overeating, food responsiveness, and enjoyment of food. This was an observational longitudinal study. Among 207 low-income children (54.6% non-Hispanic white, 46.9% girls), early childhood stress exposure was measured by parent report and a stress exposure index calculated, with higher scores indicating more stress exposure. Eating behaviors were measured in early (mean, 4.3; standard deviation, 0.5 years) and middle (mean, 7.9; standard deviation, 0.7 years) childhood. Observed EAH was assessed by measuring kilocalories of palatable food the child consumed after a meal. Parents reported on child eating behaviors on the Child Eating Behavior Questionnaire. Child weight and height were measured and body mass index z score (BMIz) calculated. Multivariable linear regression, adjusting for child sex, race/ethnicity, and BMIz, was used to examine the association of stress exposure with rate of change per year in each child eating behavior. Early childhood stress exposure predicted yearly increases in EAH (β = 0.14; 95% confidence interval, 0.002, 0.27) and Emotional Overeating (β = 0.14; 95% confidence interval, 0.008, 0.27). Stress exposure was not associated with Food Responsiveness (trend for decreased Enjoyment of Food; β = -0.13; 95% confidence interval, 0.002, -0.26). All child obesogenic eating behaviors increased with age (P < .05). Early stress exposure predicted increases in child eating behaviors known to associate with overweight/obesity. Psychosocial stress may confer overweight/obesity risk through eating behavior pathways. Targeting eating behaviors may be an important prevention strategy for children exposed to stress. Copyright © 2018 Academic Pediatric Association. Published by Elsevier Inc. All rights reserved.

  8. Linking ecophysiological modelling with quantitative genetics to support marker-assisted crop design for improved yields of rice (Oryza sativa) under drought stress.

    PubMed

    Gu, Junfei; Yin, Xinyou; Zhang, Chengwei; Wang, Huaqi; Struik, Paul C

    2014-09-01

    Genetic markers can be used in combination with ecophysiological crop models to predict the performance of genotypes. Crop models can estimate the contribution of individual markers to crop performance in given environments. The objectives of this study were to explore the use of crop models to design markers and virtual ideotypes for improving yields of rice (Oryza sativa) under drought stress. Using the model GECROS, crop yield was dissected into seven easily measured parameters. Loci for these parameters were identified for a rice population of 94 introgression lines (ILs) derived from two parents differing in drought tolerance. Marker-based values of ILs for each of these parameters were estimated from additive allele effects of the loci, and were fed to the model in order to simulate yields of the ILs grown under well-watered and drought conditions and in order to design virtual ideotypes for those conditions. To account for genotypic yield differences, it was necessary to parameterize the model for differences in an additional trait 'total crop nitrogen uptake' (Nmax) among the ILs. Genetic variation in Nmax had the most significant effect on yield; five other parameters also significantly influenced yield, but seed weight and leaf photosynthesis did not. Using the marker-based parameter values, GECROS also simulated yield variation among 251 recombinant inbred lines of the same parents. The model-based dissection approach detected more markers than the analysis using only yield per se. Model-based sensitivity analysis ranked all markers for their importance in determining yield differences among the ILs. Virtual ideotypes based on markers identified by modelling had 10-36 % more yield than those based on markers for yield per se. This study outlines a genotype-to-phenotype approach that exploits the potential value of marker-based crop modelling in developing new plant types with high yields. The approach can provide more markers for selection programmes for

  9. Linking ecophysiological modelling with quantitative genetics to support marker-assisted crop design for improved yields of rice (Oryza sativa) under drought stress

    PubMed Central

    Gu, Junfei; Yin, Xinyou; Zhang, Chengwei; Wang, Huaqi; Struik, Paul C.

    2014-01-01

    Background and Aims Genetic markers can be used in combination with ecophysiological crop models to predict the performance of genotypes. Crop models can estimate the contribution of individual markers to crop performance in given environments. The objectives of this study were to explore the use of crop models to design markers and virtual ideotypes for improving yields of rice (Oryza sativa) under drought stress. Methods Using the model GECROS, crop yield was dissected into seven easily measured parameters. Loci for these parameters were identified for a rice population of 94 introgression lines (ILs) derived from two parents differing in drought tolerance. Marker-based values of ILs for each of these parameters were estimated from additive allele effects of the loci, and were fed to the model in order to simulate yields of the ILs grown under well-watered and drought conditions and in order to design virtual ideotypes for those conditions. Key Results To account for genotypic yield differences, it was necessary to parameterize the model for differences in an additional trait ‘total crop nitrogen uptake’ (Nmax) among the ILs. Genetic variation in Nmax had the most significant effect on yield; five other parameters also significantly influenced yield, but seed weight and leaf photosynthesis did not. Using the marker-based parameter values, GECROS also simulated yield variation among 251 recombinant inbred lines of the same parents. The model-based dissection approach detected more markers than the analysis using only yield per se. Model-based sensitivity analysis ranked all markers for their importance in determining yield differences among the ILs. Virtual ideotypes based on markers identified by modelling had 10–36 % more yield than those based on markers for yield per se. Conclusions This study outlines a genotype-to-phenotype approach that exploits the potential value of marker-based crop modelling in developing new plant types with high yields. The

  10. Hydrostatic Stress Effects in Metal Plasticity

    NASA Technical Reports Server (NTRS)

    Wilson, Christopher D.

    1999-01-01

    Since the 1940s, the theory of plasticity has assumed that hydrostatic stress does not affect the yield or postyield behavior of metals. This assumption is based on the early work of Bridgman. Bridgman found that hydrostatic pressure (compressive stress) does not affect yield behavior until a substantial amount of pressure (greater than 100 ksi) is present. The objective of this study was to determine the effect of hydrostatic tension on yield behavior. Two different specimen geometries were examined: an equal-arm bend specimen and a double edge notch specimen. The presence of a notch is sufficient to develop high enough hydrostatic tensile stresses to affect yield. The von Mises yield function, which does not have a hydrostatic component, and the Drucker-Prager yield function, which includes a hydrostatic component, were used in finite element analyses of the two specimen geometries. The analyses were compared to test data from IN 100 specimens. For both geometries, the analyses using the Drucker-Prager yield function more closely simulated the test data. The von Mises yield function lead to 5-10% overprediction of the force-displacement or force-strain response of the test specimens.

  11. Increased prevalence of posttraumatic stress disorder in CRPS.

    PubMed

    Speck, V; Schlereth, T; Birklein, F; Maihöfner, C

    2017-03-01

    Although specific psychological disorders in complex regional pain syndrome (CRPS) have not been identified, studies suggest that CRPS patients may have increased rates of traumatic life events. Because these events do not always lead to apparent psychological symptoms, we systematically screened CRPS patients for posttraumatic stress disorder (PTSD) to determine if PTSD could be a risk factor for CRPS. Consecutive CRPS patients referred to two university hospital centres (University of Erlangen, UMC Mainz) between December 2011 and April 2013 were prospectively examined using a diagnostic PTSD instrument (Post-traumatic Stress Diagnostic Scale (PDS). We also tested maladaptive coping strategies (brief-COPE inventory) and the PDS severity score as predictors for CRPS. Patients with non-CRPS extremity pain and healthy individuals were used as control groups. We collected data from 152 patients with CRPS, 55 control patients and 55 age- and sex-matched healthy individuals. Fifty-eight CRPS patients (38%), six non-CRPS pain patients (10%) and two healthy individuals (4%) met diagnostic criteria for PTSD. Initial PTSD symptom onset was prior to CRPS in 50 CRPS patients (86%) and during the course of CRPS in eight patients. Results of a logistic regression revealed that the PTSD severity score was associated with CRPS (p < 0.0001). Maladaptive coping strategies (p < 0.0001) were related to PTSD. posttraumatic stress disorder (PTSD) is more frequent in patients with CRPS than it is in the general population. Research has not yet provided support for specific psychological predictors for CRPS. © 2016 European Pain Federation - EFIC®.

  12. Preconditioning L6 Muscle Cells with Naringin Ameliorates Oxidative Stress and Increases Glucose Uptake

    PubMed Central

    Dhanya, R.; Arun, K. B.; Nisha, V. M.; Syama, H. P.; Nisha, P.; Santhosh Kumar, T. R.; Jayamurthy, P.

    2015-01-01

    Enhanced oxidative stress contributes to pathological changes in diabetes and its complications. Thus, strategies to reduce oxidative stress may alleviate these pathogenic processes. Herein, we have investigated Naringin mediated regulation of glutathione (GSH) & intracellular free radical levels and modulation of glucose uptake under oxidative stress in L6 cell lines. The results from the study demonstrated a marked decrease in glutathione with a subsequent increase in free radical levels, which was reversed by the pretreatment of Naringin. We also observed that the increased malondialdehyde level, the marker of lipid peroxidation on induction of oxidative stress was retrieved on Naringin pretreatment. Addition of Naringin (100 μM) showed approximately 40% reduction in protein glycation in vitro. Furthermore, we observed a twofold increase in uptake of fluorescent labeled glucose namely 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2 - NBDG) on Naringin treatment in differentiated L6 myoblast. The increased uptake of 2-NBDG by L6 myotubes may be attributed due to the enhanced translocation of GLUT4. Our results demonstrate that Naringin activate GSH synthesis through a novel antioxidant defense mechanism against excessive Reactive Oxygen Species (ROS) production, contributing to the prevention of oxidative damage in addition to its effect on glycemic control. PMID:26147673

  13. DREB1A promotes root development in deep soil layers and increases water extraction under water stress in groundnut.

    PubMed

    Vadez, V; Rao, J S; Bhatnagar-Mathur, P; Sharma, K K

    2013-01-01

    Water deficit is a major yield-limiting factor for many crops, and improving the root system has been proposed as a promising breeding strategy, although not in groundnut (Arachis hypogaea L.). The present work was carried out mainly to assess how root traits are influenced under water stress in groundnut, whether transgenics can alter root traits, and whether putative changes lead to water extraction differences. Several transgenic events, transformed with DREB1A driven by the rd29 promoter, along with wild-type JL24, were tested in a lysimeter system that mimics field conditions under both water stress (WS) and well-watered (WW) conditions. The WS treatment increased the maximum rooting depth, although the increase was limited to about 20% in JL24, compared to 50% in RD11. The root dry weight followed a similar trend. Consequently, the root dry weight and length density of transgenics was higher in layers below 100-cm depth (Exp. 1) and below 30 cm (Exp. 2). The root diameter was unchanged under WS treatment, except a slight increase in the 60-90-cm layer. The root diameter increased below 60 cm in both treatments. In the WW treatment, total water extraction of RD33 was higher than in JL24 and other transgenic events, and somewhat lower in RD11 than in JL24. In the WS treatment, water extraction of RD2, RD11 and RD33 was higher than in JL24. These water extraction differences were mostly apparent in the initial 21 days after treatment imposition and were well related to root length density in the 30-60-cm layer (R(2) = 0.68), but not to average root length density. In conclusion, water stress promotes rooting growth more strongly in transgenic events than in the wild type, especially in deep soil layers, and this leads to increased water extraction. This opens an avenue for tapping these characteristics toward the improvement of drought adaptation in deep soil conditions, and toward a better understanding of genes involved in rooting in groundnut. © 2012

  14. Experimental evidence of impact ignition: 100-fold increase of neutron yield by impactor collision.

    PubMed

    Azechi, H; Sakaiya, T; Watari, T; Karasik, M; Saito, H; Ohtani, K; Takeda, K; Hosoda, H; Shiraga, H; Nakai, M; Shigemori, K; Fujioka, S; Murakami, M; Nagatomo, H; Johzaki, T; Gardner, J; Colombant, D G; Bates, J W; Velikovich, A L; Aglitskiy, Y; Weaver, J; Obenschain, S; Eliezer, S; Kodama, R; Norimatsu, T; Fujita, H; Mima, K; Kan, H

    2009-06-12

    We performed integrated experiments on impact ignition, in which a portion of a deuterated polystyrene (CD) shell was accelerated to about 600 km/s and was collided with precompressed CD fuel. The kinetic energy of the impactor was efficiently converted into thermal energy generating a temperature of about 1.6 keV. We achieved a two-order-of-magnitude increase in the neutron yield by optimizing the timing of the impact collision, demonstrating the high potential of impact ignition for fusion energy production.

  15. Increased sensitivity to salt stress in tocopherol-deficient Arabidopsis mutants growing in a hydroponic system

    PubMed Central

    Ellouzi, Hasna; Hamed, Karim Ben; Cela, Jana; Müller, Maren; Abdelly, Chedly; Munné-Bosch, Sergi

    2013-01-01

    Recent studies suggest that tocopherols could play physiological roles in salt tolerance but the mechanisms are still unknown. In this study, we analyzed changes in growth, mineral and oxidative status in vte1 and vte4 Arabidopsis thaliana mutants exposed to salt stress. vte1 and vte4 mutants lack α-tocopherol, but only the vte1 mutant is additionally deficient in γ-tocopherol. Results showed that a deficiency in vitamin E leads to reduced growth and increased oxidative stress in hydroponically-grown plants. This effect was observed at early stages, not only in rosettes but also in roots. The vte1 mutant was more sensitive to salt-induced oxidative stress than the wild type and the vte4 mutant. Salt sensitivity was associated with (i) high contents of Na+, (ii) reduced efficiency of PSII photochemistry (Fv/Fm ratio) and (iii) more pronounced oxidative stress as indicated by increased hydrogen peroxide and malondialdeyde levels. The vte 4 mutant, which accumulates γ- instead of α-tocopherol showed an intermediate sensitivity to salt stress between the wild type and the vte1 mutant. Contents of abscisic acid, jasmonic acid and the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid were higher in the vte1 mutant than the vte4 mutant and wild type. It is concluded that vitamin E-deficient plants show an increased sensitivity to salt stress both in rosettes and roots, therefore indicating the positive role of tocopherols in stress tolerance, not only by minimizing oxidative stress, but also controlling Na+/K+ homeostasis and hormonal balance. PMID:23299430

  16. A Theoretical Model for Estimation of Yield Strength of Fiber Metal Laminate

    NASA Astrophysics Data System (ADS)

    Bhat, Sunil; Nagesh, Suresh; Umesh, C. K.; Narayanan, S.

    2017-08-01

    The paper presents a theoretical model for estimation of yield strength of fiber metal laminate. Principles of elasticity and formulation of residual stress are employed to determine the stress state in metal layer of the laminate that is found to be higher than the stress applied over the laminate resulting in reduced yield strength of the laminate in comparison with that of the metal layer. The model is tested over 4A-3/2 Glare laminate comprising three thin aerospace 2014-T6 aluminum alloy layers alternately bonded adhesively with two prepregs, each prepreg built up of three uni-directional glass fiber layers laid in longitudinal and transverse directions. Laminates with prepregs of E-Glass and S-Glass fibers are investigated separately under uni-axial tension. Yield strengths of both the Glare variants are found to be less than that of aluminum alloy with use of S-Glass fiber resulting in higher laminate yield strength than with the use of E-Glass fiber. Results from finite element analysis and tensile tests conducted over the laminates substantiate the theoretical model.

  17. The In Vitro Mass-Produced Model Mycorrhizal Fungus, Rhizophagus irregularis, Significantly Increases Yields of the Globally Important Food Security Crop Cassava

    PubMed Central

    Ceballos, Isabel; Ruiz, Michael; Fernández, Cristhian; Peña, Ricardo

    2013-01-01

    The arbuscular mycorrhizal symbiosis is formed between arbuscular mycorrhizal fungi (AMF) and plant roots. The fungi provide the plant with inorganic phosphate (P). The symbiosis can result in increased plant growth. Although most global food crops naturally form this symbiosis, very few studies have shown that their practical application can lead to large-scale increases in food production. Application of AMF to crops in the tropics is potentially effective for improving yields. However, a main problem of using AMF on a large-scale is producing cheap inoculum in a clean sterile carrier and sufficiently concentrated to cheaply transport. Recently, mass-produced in vitro inoculum of the model mycorrhizal fungus Rhizophagus irregularis became available, potentially making its use viable in tropical agriculture. One of the most globally important food plants in the tropics is cassava. We evaluated the effect of in vitro mass-produced R. irregularis inoculum on the yield of cassava crops at two locations in Colombia. A significant effect of R. irregularis inoculation on yield occurred at both sites. At one site, yield increases were observed irrespective of P fertilization. At the other site, inoculation with AMF and 50% of the normally applied P gave the highest yield. Despite that AMF inoculation resulted in greater food production, economic analyses revealed that AMF inoculation did not give greater return on investment than with conventional cultivation. However, the amount of AMF inoculum used was double the recommended dose and was calculated with European, not Colombian, inoculum prices. R. irregularis can also be manipulated genetically in vitro, leading to improved plant growth. We conclude that application of in vitro R. irregularis is currently a way of increasing cassava yields, that there is a strong potential for it to be economically profitable and that there is enormous potential to improve this efficiency further in the future. PMID:23950975

  18. Evidence that ship noise increases stress in right whales.

    PubMed

    Rolland, Rosalind M; Parks, Susan E; Hunt, Kathleen E; Castellote, Manuel; Corkeron, Peter J; Nowacek, Douglas P; Wasser, Samuel K; Kraus, Scott D

    2012-06-22

    Baleen whales (Mysticeti) communicate using low-frequency acoustic signals. These long-wavelength sounds can be detected over hundreds of kilometres, potentially allowing contact over large distances. Low-frequency noise from large ships (20-200 Hz) overlaps acoustic signals used by baleen whales, and increased levels of underwater noise have been documented in areas with high shipping traffic. Reported responses of whales to increased noise include: habitat displacement, behavioural changes and alterations in the intensity, frequency and intervals of calls. However, it has been unclear whether exposure to noise results in physiological responses that may lead to significant consequences for individuals or populations. Here, we show that reduced ship traffic in the Bay of Fundy, Canada, following the events of 11 September 2001, resulted in a 6 dB decrease in underwater noise with a significant reduction below 150 Hz. This noise reduction was associated with decreased baseline levels of stress-related faecal hormone metabolites (glucocorticoids) in North Atlantic right whales (Eubalaena glacialis). This is the first evidence that exposure to low-frequency ship noise may be associated with chronic stress in whales, and has implications for all baleen whales in heavy ship traffic areas, and for recovery of this endangered right whale population.

  19. Evidence that ship noise increases stress in right whales

    PubMed Central

    Rolland, Rosalind M.; Parks, Susan E.; Hunt, Kathleen E.; Castellote, Manuel; Corkeron, Peter J.; Nowacek, Douglas P.; Wasser, Samuel K.; Kraus, Scott D.

    2012-01-01

    Baleen whales (Mysticeti) communicate using low-frequency acoustic signals. These long-wavelength sounds can be detected over hundreds of kilometres, potentially allowing contact over large distances. Low-frequency noise from large ships (20–200 Hz) overlaps acoustic signals used by baleen whales, and increased levels of underwater noise have been documented in areas with high shipping traffic. Reported responses of whales to increased noise include: habitat displacement, behavioural changes and alterations in the intensity, frequency and intervals of calls. However, it has been unclear whether exposure to noise results in physiological responses that may lead to significant consequences for individuals or populations. Here, we show that reduced ship traffic in the Bay of Fundy, Canada, following the events of 11 September 2001, resulted in a 6 dB decrease in underwater noise with a significant reduction below 150 Hz. This noise reduction was associated with decreased baseline levels of stress-related faecal hormone metabolites (glucocorticoids) in North Atlantic right whales (Eubalaena glacialis). This is the first evidence that exposure to low-frequency ship noise may be associated with chronic stress in whales, and has implications for all baleen whales in heavy ship traffic areas, and for recovery of this endangered right whale population. PMID:22319129

  20. Salt Stress Increases the Level of Translatable mRNA for Phosphoenolpyruvate Carboxylase in Mesembryanthemum crystallinum1

    PubMed Central

    Ostrem, James A.; Olson, Steve W.; Schmitt, Jürgen M.; Bohnert, Hans J.

    1987-01-01

    Mesembryanthemum crystallinum responds to salt stress by switching from C3 photosynthesis to Crassulacean acid metabolism (CAM). During this transition the activity of phosphoenolpyruvate carboxylase (PEPCase) increases in soluble protein extracts from leaf tissue. We monitored CAM induction in plants irrigated with 0.5 molar NaCl for 5 days during the fourth, fifth, and sixth week after germination. Our results indicate that the age of the plant influenced the response to salt stress. There was no increase in PEPCase protein or PEPCase enzyme activity when plants were irrigated with 0.5 molar NaCl during the fourth and fifth week after germination. However, PEPCase activity increased within 2 to 3 days when plants were salt stressed during the sixth week after germination. Immunoblot analysis with anti-PEPCase antibodies showed that PEPCase synthesis was induced in both expanded leaves and in newly developing axillary shoot tissue. The increase in PEPCase protein was paralleled by an increase in PEPCase mRNA as assayed by immunoprecipitation of PEPCase from the in vitro translation products of RNA from salt-stressed plants. These results demonstrate that salinity increased the level of PEPCase in leaf and shoot tissue via a stress-induced increase in the steady-state level of translatable mRNA for this enzyme. Images Fig. 2 Fig. 3 Fig. 4 PMID:16665596

  1. Working the night shift causes increased vascular stress and delayed recovery in young women.

    PubMed

    Lo, Shih-Hsiang; Lin, Lian-Yu; Hwang, Jing-Shiang; Chang, Yu-Yin; Liau, Chiau-Suong; Wang, Jung-Der

    2010-08-01

    Shiftwork has been associated with elevated blood pressure (BP) and decreased heart-rate variability (HRV), factors that may increase the long-term risk of cardiovascular-related mortality and morbidity. This study explored the effect of shiftwork on dynamic changes in autonomic control of HRV (cardiac stress), systolic BP and diastolic BP, i.e., SBP and DBP (vascular stress), and recovery in the same subjects working different shifts. By studying the same subjects, the authors could reduce the effect of possible contribution of between-subject variation from genetic predisposition and environmental factors. The authors recruited 16 young female nurses working rotating shifts--day (08:00-16:00 h), evening (16:00-00:00 h), and night (00:00-08:00 h)--and 6 others working the regular day shift. Each nurse received simultaneous and repeated 48-h ambulatory electrocardiography and BP monitoring during their work day and the following off-duty day. Using a linear mixed-effect model to adjust for day shift, the results of the repeated-measurements and self-comparisons found significant shift differences in vascular stress. While working the night shift, the nurses showed significant increases in vascular stress, with increased SBP of 9.7 mm Hg. The changes of SBP and DBP seemed to peak during waking time at the same time on the day off as they did on the working day. Whereas HRV profiles usually returned to baseline level after each shift, the SBP and DBP of night-shift workers did not completely return to baseline levels the following off-duty day (p < .001). The authors concluded that although the nurses may recover from cardiac stress the first day off following a night shift, they do not completely recover from increases in vascular stress on that day.

  2. The influence of rheological weakening and yield stress on the interaction of slabs with the 670 km discontinuity

    NASA Astrophysics Data System (ADS)

    Čížková, Hana; van Hunen, Jeroen; van den Berg, Arie P.; Vlaar, Nico J.

    2002-06-01

    Results of high resolution seismic tomography showing subducting slabs deflected in the transition zone and thickened in the lower mantle seem to call for slab material weaker than inferred from mineral physics deformation mechanisms. A possible mechanism suggested by several authors could be the weakening due to grain size reduction, which should occur in the cold portion of fast slabs after an exothermic phase transition at a depth of 400 km. Since the amount of weakening as well as the rate of subsequent strengthening due to the grain growth are not precisely known, we present here a parametric study of slab behavior in the transition zone and upper part of the lower mantle. We simulate a subducting slab in a two-dimensional (2-D) Cartesian box in the numerical model with composite rheology including diffusion creep, dislocation creep and a general stress limiting rheology approximating Peierl's creep. We concentrate on two rheologic effects: the dynamic effect of slab weakening due to grain size reduction at the phase boundary and the effect of yield stress of stress limiting rheology. The effect of trench migration on slab deformation is also included in our study. Results show that the slab ability to penetrate into the lower mantle is not significantly affected by a trench retreat in the absence of grain size weakening. However, in case of a 4 cm/yr trench retreat, grain size weakening provides a viable mechanism to deflect the slab in the transition zone, provided that stress limiting deformation mechanism would limit the effective viscosity outside the areas of grain size weakening to about 1024 Pa s.

  3. Chronic stress increases vulnerability to diet-related abdominal fat, oxidative stress, and metabolic risk.

    PubMed

    Aschbacher, Kirstin; Kornfeld, Sarah; Picard, Martin; Puterman, Eli; Havel, Peter J; Stanhope, Kimber; Lustig, Robert H; Epel, Elissa

    2014-08-01

    In preclinical studies, the combination of chronic stress and a high sugar/fat diet is a more potent driver of visceral adiposity than diet alone, a process mediated by peripheral neuropeptide Y (NPY). In a human model of chronic stress, we investigated whether the synergistic combination of highly palatable foods (HPF; high sugar/fat) and stress was associated with elevated metabolic risk. Using a case-control design, we compared 33 post-menopausal caregivers (the chronic stress group) to 28 age-matched low-stress control women on reported HPF consumption (modified Block Food Frequency Questionnaire), waistline circumference, truncal fat ultrasound, and insulin sensitivity using a 3-h oral glucose tolerance test. A fasting blood draw was assayed for plasma NPY and oxidative stress markers (8-hydroxyguanosine and F2-Isoprostanes). Among chronically stressed women only, greater HPF consumption was associated with greater abdominal adiposity, oxidative stress, and insulin resistance at baseline (all p's≤.01). Furthermore, plasma NPY was significantly elevated in chronically stressed women (p<.01), and the association of HPF with abdominal adiposity was stronger among women with high versus low NPY. There were no significant predictions of change over 1-year, likely due to high stability (little change) in the primary outcomes over this period. Chronic stress is associated with enhanced vulnerability to diet-related metabolic risk (abdominal adiposity, insulin resistance, and oxidative stress). Stress-induced peripheral NPY may play a mechanistic role. Copyright © 2014. Published by Elsevier Ltd.

  4. Slope Controls Grain Yield and Climatic Yield in Mountainous Yunnan province, China

    NASA Astrophysics Data System (ADS)

    Duan, X.; Rong, L.; Gu, Z.; Feng, D.

    2017-12-01

    Mountainous regions are increasingly vulnerable to food insecurity because of limited arable land, growing population pressure, and climate change. Development of sustainable mountain agriculture will require an increased understanding of the effects of environmental factors on grain and climatic yields. The objective of this study was to explore the relationships between actual grain yield, climatic yield, and environmental factors in a mountainous region in China. We collected data on the average grain yield per unit area in 119 counties in Yunnan province from 1985 to 2012, and chose 17 environmental factors for the same period. Our results showed that actual grain yield ranged from 1.43 to 6.92 t·ha-1, and the climatic yield ranged from -0.15 to -0.01 t·ha-1. Lower climatic yield but higher grain yield was generally found in central areas and at lower slopes and elevations in the western and southwestern counties of Yunnan province. Higher climatic yield but lower grain yield were found in northwestern parts of Yunnan province on steep slopes. Annual precipation and temperature had a weak influence on the climatic yield. Slope explained 44.62 and 26.29% of the variation in grain yield and climatic yield. The effects of topography on grain and climatic yields were greater than climatic factors. Slope was the most important environmental variable for the variability in climatic and grain yields in the mountainous Yunnan province due to the highly heterogeneous topographic conditions. Conversion of slopes to terraces in areas with higher climatic yields is an effective way to maintain grain production in response to climate variability. Additionally, soil amendments and soil and water conservation measures should be considered to maintain soil fertility and aid in sustainable development in central areas, and in counties at lower slopes and elevations in western and southwestern Yunnan province.

  5. Ozone Exposure Increases Circulating Stress Hormones and Lipid Metabolites in Humans

    EPA Science Inventory

    RATIONALE: Air pollution has been associated with increased prevalence of type 2 diabetes; however, the mechanisms remain unknown. We have shown that acute ozone exposure in rats induces release of stress hormones, hyperglycemia, leptinemia, and gluoose intolerance that are assoc...

  6. A microstructure-based yield stress and work-hardening model for textured 6xxx aluminium alloys

    NASA Astrophysics Data System (ADS)

    Khadyko, M.; Myhr, O. R.; Dumoulin, S.; Hopperstad, O. S.

    2016-04-01

    The plastic properties of an aluminium alloy are defined by its microstructure. The most important factors are the presence of alloying elements in the form of solid solution and precipitates of various sizes, and the crystallographic texture. A nanoscale model that predicts the work-hardening curves of 6xxx aluminium alloys was proposed by Myhr et al. The model predicts the solid solution concentration and the particle size distributions of different types of metastable precipitates from the chemical composition and thermal history of the alloy. The yield stress and the work hardening of the alloy are then determined from dislocation mechanics. The model was largely used for non-textured materials in previous studies. In this work, a crystal plasticity-based approach is proposed for the work hardening part of the nanoscale model, which allows including the influence of the crystallographic texture. The model is evaluated by comparison with experimental data from uniaxial tensile tests on two textured 6xxx alloys in five temper conditions.

  7. Acute psychological stress increases plasma levels of cortisol, prolactin and TSH.

    PubMed

    Schedlowski, M; Wiechert, D; Wagner, T O; Tewes, U

    1992-01-01

    The effects of acute stress during a parachute jump on hormonal responses were studied in 12 experienced and 11 inexperienced military parachutists. Each subject performed two jumps. Prior to and immediately after each jump blood samples were drawn and analysed for plasma levels of cortisol, prolactin, thyrotropin (TSH), somatotropin (STH), and luteinizing hormone (LH). While there was a significant increase in cortisol, prolactin and TSH levels after both jumps, no alterations could be observed in STH and LH levels. Stress-induced hormonal responses were not affected by jump experience. There was also no association between the endocrine variables and anxiety scores.

  8. Progesterone pretreatment increases the stress response to social isolation in ewes.

    PubMed

    Freitas-de-Melo, Aline; Damián, Juan Pablo; Hötzel, Maria José; Banchero, Georgget; Ungerfeld, Rodolfo

    2016-01-01

    In rodents, progesterone (P4) pretreatment increases anxiety and response to stressors. Social isolation is a stressor that generates physiological and behavioural stress responses in sheep. The aim of the study was to compare the stress response of anoestrous ewes previously treated or not with P4 to the social isolation test. Ten ewes received P4 treatment during 13 d (group P4-W) and another 10 remained untreated as controls (group Con). The ewes were individually isolated in a novel place during 10 min, 24 h after the end of P4 pretreatment and their behaviours were recorded. Cortisol and P4 concentrations as well as body surface temperature were recorded before and after the test. Ewes of the P4-W group presented higher cortisol levels 0, 10, 20 and 30 min after the social isolation and had greater area under the curve of cortisol compared to Con ewes (41,785%±4,156% vs. 25,682%±4,565% during 75 min). Progesterone and body surface temperature increased after social isolation, with no differences between P4-W and Con ewes. There were no differences in behavioural responses to social isolation. P4 pretreatment appears to augment the stress response to social isolation in anoestrous ewes.

  9. Acute mental stress but not enforced muscle activity transiently increases natural cytotoxicity in spontaneously hypertensive rats.

    PubMed

    Jonsdottir, I H; Johansson, C; Asea, A; Hellstrand, K; Hoffmann, P

    1996-08-01

    The influence of acute mental stress and the effect of electrically induced skeletal muscle contractions on natural cytotoxicity in vivo was investigated in spontaneously hypertensive rats Natural cytotoxicity in vivo was measured as the clearance of injected 51Cr-labelled YAC-1 lymphoma cells from the lungs, which are specifically lysed by natural killer cells. The mental stress consisted of an air jet directed towards the animals in their cage for 25 min. During the mental stress there was a significant increase in natural cytotoxicity. Thus, retained radioactivity in the lungs was decreased to 74 +/- 6% of the control levels which was set to 100% (P < 0.01). This augmentation of YAC-1-cell clearance could be blocked with the beta-adrenergic receptor antagonist Timolol. Two hours after termination of the air stress, in vivo cytotoxicity had returned to control levels. In contrast, acute physical stress, consisting of electrically induced muscle contractions for 60 min, had no significant effects on in vivo cytotoxicity, either during the stimulation or 1, 2 or 24 h after the stimulation. Further, significantly increased plasma levels of adrenaline were seen after the air jet stress, but not after muscle stimulation. There were no significant changes in plasma noradrenaline levels either after air stress or muscle stimulation. These results indicate that changes in in vivo cytotoxicity after mild mental stress are dependent on increased plasma catecholamine levels while acute physical stress without changes in catecholamine levels, does not influence in vivo cytotoxicity.

  10. Analytical Yield Criterion for an Anisotropic Material Containing Spherical Voids and Exhibiting Tension-Compression Asymmetry

    DTIC Science & Technology

    2011-11-01

    where s1, s2 and s3 are the principal values of the stress deviator. The material parameter k captures strength differential effects while a is the...condition can be written as re ¼ rT1; ð11Þ where re is the effective stress associated to the yield function of Eq. (9) and rT1 is the uniaxial tensile...CPB06 yield cri- terion reduces to that of Hill (1948). 3.1. Local stress potential For a = 2, the effective stress of Eq. (12) becomes re ¼ m̂

  11. An experimental examination of the effort-reward imbalance model of occupational stress: Increased financial reward is related to reduced stress physiology.

    PubMed

    Landolt, Kathleen; O'Donnell, Emma; Hazi, Agnes; Dragano, Nico; Wright, Bradley J

    2017-04-01

    Effort-reward imbalance in the workplace is linked to a variety of negative health and organisational outcomes, but it has rarely been assessed experimentally. We manipulated reward (while keeping effort constant) in a within-subjects design with female participants (N=60) who were randomly assigned to high and standard reward conditions within a simulated office environment. Self-report, behavioural (task performance), and physiological (heart rate variability, salivary alpha amylase) measures assessed the impact of increased financial reward. Participants reported increased perceptions of reward, performed moderately better on the task, and were less physiologically reactive in the high reward versus the standard condition. These findings highlight the importance of assessing both subjective self-reports of stress together with objective physiological measures of stress, and suggest that increasing monetary rewards has the potential to decrease stress physiological reactivity, and in turn, reduce the risk of ill-health in employees, and may also positively influence task efficacy. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. Low-Temperature Stress during Capped Brood Stage Increases Pupal Mortality, Misorientation and Adult Mortality in Honey Bees

    PubMed Central

    Wang, Qing; Xu, Xinjian; Zhu, Xiangjie; Chen, Lin; Zhou, Shujing; Huang, Zachary Y.; Zhou, Bingfeng

    2016-01-01

    Honey bees (Apis mellifera) are key pollinators, playing a vital role in ecosystem maintenance and stability of crop yields. Recently, reduced honey bee survival has attracted intensive attention. Among all other honey bee stresses, temperature is a fundamental ecological factor that has been shown to affect honey bee survival. Yet, the impact of low temperature stress during capped brood on brood mortality has not been systematically investigated. In addition, little was known about how low temperature exposure during capped brood affects subsequent adult longevity. In this study, capped worker broods at 12 different developmental stages were exposed to 20°C for 12, 24, 36, 48, 60, 72, 84 and 96 hours, followed by incubation at 35°C until emergence. We found that longer durations of low temperature during capped brood led to higher mortality, higher incidences of misorientation inside cells and shorter worker longevity. Capped brood as prepupae and near emergence were more sensitive to low-temperature exposure, while capped larvae and mid-pupal stages showed the highest resistance to low-temperature stress. Our results suggest that prepupae and pupae prior to eclosion are the most sensitive stages to low temperature stress, as they are to other stresses, presumably due to many physiological changes related to metamorphosis happening during these two stages. Understanding how low-temperature stress affects honey bee physiology and longevity can improve honey bee management strategies. PMID:27149383

  13. Effect of methyl jasmonate, salicylic acid, Headline and Stadium on root yield, sucrose yield, and storage properties

    USDA-ARS?s Scientific Manuscript database

    Methyl jasmonate (MeJA) and salicylic acid (SA) have been reported to enhance yield and protect crop plants and products against abiotic stresses and diseases. The effect of these compounds on sugarbeets, however, is unknown. Research was conducted in 2016 and 2017 to investigate the effects of an e...

  14. Looking for Water in the Woods: Quantifying the Potential for Forest Management to Increase Regional Water Yield

    NASA Astrophysics Data System (ADS)

    Acharya, S.; Kaplan, D. A.; Mclaughlin, D. L.; Cohen, M. J.

    2014-12-01

    Water scarcity presents a crucial challenge for water resource managers charged with maintaining hydrologic resources for domestic, industrial, and agricultural use while protecting natural systems. Forest lands are critical to the functioning of the hydrologic cycle in many watersheds, affecting the quantity, quality, and timing of water delivered to surface and groundwater systems. While the hydrologic impacts of forest growth and removal have been shown to be substantial in watersheds around the globe, data and models connecting forest management to water use and regional hydrology are generally lacking. We propose that water-focused forest management has the potential to deliver a "new" source of water to surface and groundwater resources. To test this hypothesis, we developed a statistical model of water yield in southeastern US pine stands as a function of forest stand structure and ecosystem water use. Model results suggest a potential increase in water yield of up to 64% for pine stands managed at lower basal areas relative to those managed according to standard silvicultural practices. At the watershed scale, the magnitude of this potential water yield enhancement is driven by existing land use and forest management; evaluated for a large watershed in NE Florida, this potential increase is in excess of 200 million gallons per day (equivalent to 20% of the anthropogenic water use in the watershed). While useful for exploration, our statistical model also highlighted critical sources of uncertainty, including the effects of climatic variation, between-site variability, water use in young pine stands, and prescribed fire. Thus, in ongoing work we are comparing the effects of specific land management actions (e.g., thinning, clearcutting, and fire) on water yield across a gradient of environmental conditions (soil type, aquifer confinement, and climate) using a novel combination of in-situ soil moisture and groundwater monitoring. These data are being used to

  15. Increased Susceptibility to Allergic Asthma with the Impairment of Respiratory Tolerance Caused by Psychological Stress.

    PubMed

    Kawano, Tasuku; Ouchi, Ryusuke; Ishigaki, Takahiro; Masuda, Chiaki; Miyasaka, Tomomitsu; Ohkawara, Yuichi; Ohta, Nobuo; Takayanagi, Motoaki; Takahashi, Tomoko; Ohno, Isao

    2018-06-06

    Bronchial asthma is characterized by type 2 T helper (Th2) cell inflammation, essentially due to a breakdown of immune tolerance to harmless environmental allergens. Etiologically, experiences of psychological stress can be associated with a heightened prevalence of asthma. However, the mechanisms underlying stress-related asthma development are unclear. In this study, we examined whether psychological stress increases susceptibility to allergic asthma by downregulating immune tolerance. Female BALB/c mice were sensitized with ovalbumin/alum, followed by ovalbumin inhalation. Ovalbumin inhalation induced immune tolerance before sensitization occurred. Some mice were exposed to restraint stress during tolerance induction or sensitization. Asthma development was evaluated by airway responsiveness, inflammation, cytokine expression, and IgE synthesis. Sensitization was evaluated by measuring proliferation and cytokine production by splenocytes. The effects of stress exposure on the numbers and functions of dendritic cells and regulatory T (Treg) cells in bronchial lymph nodes and spleens were evaluated. To investigate the role of endogenous glucocorticoid in inhibiting immune tolerance after stress exposure, we examined the effects of (i) a glucocorticoid-receptor antagonist administered prior to stress exposure, and (ii) exogenous gluco-corticoid (instead of stress exposure). Asthmatic responses and Th2-biased sensitization, which were suppressed in tolerized mice, re-emerged in tolerized mice stressed during tolerance induction in association with decreased tolerogenic dendritic and Treg cell numbers. The effects of stress exposure on tolerized mice were abolished by administering a glucocorticoid-receptor antagonist and reproduced by administering exogenous glucocorticoid without stress. Our findings suggested that psychological stress can potentially increase allergic asthma susceptibility by inhibiting immune tolerance. © 2018 S. Karger AG, Basel.

  16. Increased red cell distribution width in Fanconi anemia: a novel marker of stress erythropoiesis.

    PubMed

    Sousa, Rosa; Gonçalves, Cristina; Guerra, Isabel Couto; Costa, Emília; Fernandes, Ana; do Bom Sucesso, Maria; Azevedo, Joana; Rodriguez, Alfredo; Rius, Rocio; Seabra, Carlos; Ferreira, Fátima; Ribeiro, Letícia; Ferrão, Anabela; Castedo, Sérgio; Cleto, Esmeralda; Coutinho, Jorge; Carvalho, Félix; Barbot, José; Porto, Beatriz

    2016-07-25

    Red cell distribution width (RDW), a classical parameter used in the differential diagnosis of anemia, has recently been recognized as a marker of chronic inflammation and high levels of oxidative stress (OS). Fanconi anemia (FA) is a genetic disorder associated to redox imbalance and dysfunctional response to OS. Clinically, it is characterized by progressive bone marrow failure, which remains the primary cause of morbidity and mortality. Macrocytosis and increased fetal hemoglobin, two indicators of bone marrow stress erythropoiesis, are generally the first hematological manifestations to appear in FA. However, the significance of RDW and its possible relation to stress erythropoiesis have never been explored in FA. In the present study we analyzed routine complete blood counts from 34 FA patients and evaluated RDW, correlating with the hematological parameters most consistently associated with the FA phenotype. We showed, for the first time, that RDW is significantly increased in FA. We also showed that increased RDW is correlated with thrombocytopenia, neutropenia and, most importantly, highly correlated with anemia. Analyzing sequential hemograms from 3 FA patients with different clinical outcomes, during 10 years follow-up, we confirmed a consistent association between increased RDW and decreased hemoglobin, which supports the postulated importance of RDW in the evaluation of hematological disease progression. This study shows, for the first time, that RDW is significantly increased in FA, and this increment is correlated with neutropenia, thrombocytopenia, and highly correlated with anemia. According to the present results, it is suggested that increased RDW can be a novel marker of stress erythropoiesis in FA.

  17. Measuring heterogenous stress fields in a 3D colloidal glass

    NASA Astrophysics Data System (ADS)

    Lin, Neil; Bierbaum, Matthew; Bi, Max; Sethna, James; Cohen, Itai

    Glass in our common experience is hard and fragile. But it still bends, yields, and flows slowly under loads. The yielding of glass, a well documented yet not fully understood flow behavior, is governed by the heterogenous local stresses in the material. While resolving stresses at the atomic scale is not feasible, measurements of stresses at the single particle level in colloidal glasses, a widely used model system for atomic glasses, has recently been made possible using Stress Assessment from Local Structural Anisotropy (SALSA). In this work, we use SALSA to visualize the three dimensional stress network in a hard-sphere glass during start-up shear. By measuring the evolution of this stress network we identify local-yielding. We find that these local-yielding events often require only minimal structural rearrangement and as such have most likely been ignored in previous analyses. We then relate these micro-scale yielding events to the macro-scale flow behavior observed using bulk measurements.

  18. Are GM Crops for Yield and Resilience Possible?

    PubMed

    Paul, Matthew J; Nuccio, Michael L; Basu, Shib Sankar

    2018-01-01

    Crop yield improvements need to accelerate to avoid future food insecurity. Outside Europe, genetically modified (GM) crops for herbicide- and insect-resistance have been transformative in agriculture; other traits have also come to market. However, GM of yield potential and stress resilience has yet to impact on food security. Genes have been identified for yield such as grain number, size, leaf growth, resource allocation, and signaling for drought tolerance, but there is only one commercialized drought-tolerant GM variety. For GM and genome editing to impact on yield and resilience there is a need to understand yield-determining processes in a cell and developmental context combined with evaluation in the grower environment. We highlight a sugar signaling mechanism as a paradigm for this approach. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. A scalable satellite-based crop yield mapper: Integrating satellites and crop models for field-scale estimation in India

    NASA Astrophysics Data System (ADS)

    Jain, M.; Singh, B.; Srivastava, A.; Lobell, D. B.

    2015-12-01

    Food security will be challenged over the upcoming decades due to increased food demand, natural resource degradation, and climate change. In order to identify potential solutions to increase food security in the face of these changes, tools that can rapidly and accurately assess farm productivity are needed. With this aim, we have developed generalizable methods to map crop yields at the field scale using a combination of satellite imagery and crop models, and implement this approach within Google Earth Engine. We use these methods to examine wheat yield trends in Northern India, which provides over 15% of the global wheat supply and where over 80% of farmers rely on wheat as a staple food source. In addition, we identify the extent to which farmers are shifting sow date in response to heat stress, and how well shifting sow date reduces the negative impacts of heat stress on yield. To identify local-level decision-making, we map wheat sow date and yield at a high spatial resolution (30 m) using Landsat satellite imagery from 1980 to the present. This unique dataset allows us to examine sow date decisions at the field scale over 30 years, and by relating these decisions to weather experienced over the same time period, we can identify how farmers learn and adapt cropping decisions based on weather through time.

  20. Studies on laws of stress-magnetization based on magnetic memory testing technique

    NASA Astrophysics Data System (ADS)

    Ren, Shangkun; Ren, Xianzhi

    2018-03-01

    Metal magnetic memory (MMM) testing technique is a novel testing method which can early test stress concentration status of ferromagnetic components. Under the different maximum tensile stress, the relationship between the leakage magnetic field of at certain point of cold rolled steel specimen and the tensile stress was measured during the process of loading and unloading by repeated. It shows that when the maximum tensile stress is less than 610 MPa, the relationship between the magnetic induction intensity and the stress is linear; When the maximum tensile stress increase from 610 MPa to 653 MPa of yield point, the relationship between the magnetic induction intensity and the tensile becomes bending line. The location of the extreme point of the bending line will move rapidly from the position of smaller stress to the larger stress position, and the variation of magnetic induction intensity increases rapidly. When the maximum tensile stress is greater than the 653 MPa of yield point, the variation of the magnetic induction intensity remains large, and the position of the extreme point moves very little. In theoretical aspects, tensile stress is to be divided into ordered stress and disordered stress. In the stage of elastic stress, a microscopic model of the order stress magnetization is established, and the conclusions are in good agreement with the experimental data. In the plastic deformation stage, a microscopic model of disordered stress magnetization is established, and the conclusions are in good agreement with the experimental data, too. The research results can provide reference for the accurate quantitative detection and evaluation of metal magnetic memory testing technology.

  1. Robust features of future climate change impacts on sorghum yields in West Africa

    NASA Astrophysics Data System (ADS)

    Sultan, B.; Guan, K.; Kouressy, M.; Biasutti, M.; Piani, C.; Hammer, G. L.; McLean, G.; Lobell, D. B.

    2014-10-01

    adaptation to ongoing climate changes. Easing nitrogen stress via increasing fertilizer inputs would increase absolute yields, but also make the crops more responsive to climate stresses, thus enhancing the negative impacts of climate change in a relative sense. Finally, CO2 fertilization would significantly offset the negative climate impacts on sorghum yields by about 10%, with drier regions experiencing the largest benefits, though the net impacts of climate change remain negative even after accounting for CO2.

  2. Cell stress and translational inhibitors transiently increase the abundance of mammalian SINE transcripts.

    PubMed Central

    Liu, W M; Chu, W M; Choudary, P V; Schmid, C W

    1995-01-01

    The abundance of Alu RNA is transiently increased by heat shock in human cell lines. This effect is specific to Alu repeats among Pol III transcribed genes, since the abundance of 7SL, 7SK, 5S and U6 RNAs is essentially unaffected by heat shock. The rapid induction of Alu expression precedes the heat shock induction of mRNAs for the ubiquitin and HSP 70 heat shock genes. Heat shock mimetics also transiently induce Alu expression indicating that increased Alu expression is a general cell-stress response. Cycloheximide treatment rapidly and transiently increases the abundance of Alu RNA. Again, compared with other genes transcribed by Pol III, this increase is specific to Alu. However, as distinguished from the cell stress response, cycloheximide does not induce expression of HSP 70 and ubiquitin mRNAs. Puromycin also increases Alu expression, suggesting that this response is generally caused by translational inhibition. The response of mammalian SINEs to cell stress and translational inhibition is not limited to SINEs which are Alu homologues. Heat shock and cycloheximide each transiently induce Pol III directed expression of B1 and B2 RNAs in mouse cells and C-element RNA in rabbit cells. Together, these three species exemplify the known SINE composition of placental mammals, suggesting that mammalian SINEs are similarly regulated and may serve a common function. Images PMID:7784180

  3. Vacuolar deposition of recombinant proteins in plant vegetative organs as a strategy to increase yields.

    PubMed

    Marin Viegas, Vanesa Soledad; Ocampo, Carolina Gabriela; Petruccelli, Silvana

    2017-05-04

    Delivery of recombinant proteins to vegetative tissue vacuoles was considered inconvenient since this compartment was expected to be hydrolytic; nevertheless there is growing evidence that certain foreign proteins accumulate at high yields in vacuoles. For example avidin, cellulolytic enzymes, endolysin, and transglutaminases were produced at high yields when were sorted to leaf central vacuole avoiding the detrimental effect of these proteins on plant growth. Also, several secretory mammalian proteins such as collagen, α1-proteinase inhibitor, complement-5a, interleukin-6 and immunoglobulins accumulated at higher yields in leaf vacuoles than in the apoplast or cytosol. To reach this final destination, fusions to sequence specific vacuolar sorting signals (ssVSS) typical of proteases or proteinase inhibitors and/or Ct-VSS representative of storage proteins or plant lectins were used and both types of motifs were capable to increase accumulation. Importantly, the type of VSSs or position, either the N or C-terminus, did not alter protein stability, levels or pos-translational modifications. Vacuolar sorted glycoproteins had different type of oligosaccharides indicating that foreign proteins reached the vacuole by 2 different pathways: direct transport from the ER, bypassing the Golgi (high mannose oligosaccharides decorated proteins) or trafficking through the Golgi (Complex oligosaccharide containing proteins). In addition, some glycoproteins lacked of paucimannosidic oligosaccharides suggesting that vacuolar trimming of glycans did not occur. Enhanced accumulation of foreign proteins fused to VSS occurred in several plant species such as tobacco, Nicotiana benthamiana, sugarcane, tomato and in carrot and the obtained results were influenced by plant physiological state. Ten different foreign proteins fused to vacuolar sorting accumulated at higher levels than their apoplastic or cytosolic counterparts. For proteins with cytotoxic effects vacuolar sorted forms

  4. Interactive effects of water and controlled release urea on nitrogen metabolism, accumulation, translocation, and yield in summer maize

    NASA Astrophysics Data System (ADS)

    Li, Guanghao; Zhao, Bin; Dong, Shuting; Zhang, Jiwang; Liu, Peng; Vyn, Tony J.

    2017-10-01

    To investigate the interactive effects of water and N from controlled release urea (CRU) on N metabolism, accumulation, translocation, and yield in Zhengdan958 (a summer maize cultivar planted widely in China), three water levels (adequate water W3, mild water stress W2, severe water stress W1) and four amounts of CRU (N) (N0, N1, N2, and N3 were 0, 105, 210, and 315 kg N ha-1, respectively) were carried out under the waterproof shed and soil column conditions. The results showed that yield, N metabolism, accumulation, and translocation were significantly affected by water, CRU, and their interactions after tasseling. Yields showed an increasing trend in response to N rates from 100.2 to 128.8 g plant-1 under severe water stress (W1), from 124.7 to 174.6 g plant-1 under mild water stress (W2), and from 143.7 to 177.0 g plant-1 under adequate water conditions (W3). There was an associated optimum amount of N for each water level. Under W1 and W2, N3 treatments showed significant advantages in three N metabolism enzymes' activities and the N accumulations, and yield and its components were highest. But the nitrogen harvest index (NHI) of N3 had no significant difference with other nitrogen treatments. Under W3, the N translocation efficiency (NTE) and N translocation conversion rate (NTCR) of N2 in stem and leaf were higher than those of N3, but the N metabolism enzymes' activities and yields of N2 and N3 had no significant difference, which indicated that N2 was superior to N3. The N3 treatment under W2 and N2 under W3 increased the N accumulation capacity in maize grain as well as the N translocation to grain that contributed to the increase of 1000-gain weight and grains per ear after tasseling. Under this experimental condition, a CRU rate of 225 kg ha-1 was the best treatment when the soil moisture content was 75 ± 5% of field capacity, but an N rate of 300 kg ha-1 was superior when soil moisture content was maintained at 55 ± 5% of field capacity during the

  5. Continental-scale Sensitivity of Water Yield to Changes in Impervious Cover

    NASA Astrophysics Data System (ADS)

    Caldwell, P.; Sun, G.; McNulty, S.; Cohen, E.; Moore Myers, J.

    2012-12-01

    Projected land conversion from native forest, grassland, and shrubland to urban impervious cover will alter watershed water balances by reducing groundwater recharge and evapotranspiration, increasing surface runoff, and potentially altering regional weather patterns. These hydrologic changes have important ecohydrological implications to local watersheds, including stream channel habitat degradation and the loss of aquatic biodiversity. Many observational studies have evaluated the impact of urbanization on water yield in small catchments downstream of specific urban areas. However it is often difficult to separate the impact of impervious cover from other impacts of urbanization such as leaking water infrastructure, irrigation runoff, water supply withdrawals, and effluent discharge. In addition, the impact of impervious cover has not been evaluated at scales large enough to assess spatial differences in water yield sensitivity to changes in impervious cover. The objective of this study was to assess the sensitivity of water yield to impervious cover across the conterminous U.S., and to identify locations where water yield will be most impacted by future urbanization. We used the Water Supply Stress Index (WaSSI) model to simulate monthly water yield as impacted by impervious cover for the approximately 82,000 12-digit HUC watersheds across the conterminous U.S. WaSSI computed infiltration, surface runoff, soil moisture, and baseflow processes explicitly for ten vegetative land cover classes and impervious cover in each watershed using the 2006 National Land Cover Dataset estimates of impervious cover. Our results indicate that impervious cover has increased total water yield in urban areas (relative to native vegetation), and that the increase was most significant during the growing season. The proportion of stream flow that occurred as baseflow decreased, even though total water yield increased as a result of impervious cover. Water yield was most sensitive to

  6. Is current irrigation sustainable in the United States? An integrated assessment of climate change impact on water resources and irrigated crop yields

    DOE PAGES

    Blanc, Elodie; Caron, Justin; Fant, Charles; ...

    2017-06-27

    While climate change impacts on crop yields has been extensively studied, estimating the impact of water shortages on irrigated crop yields is challenging because the water resources management system is complex. To investigate this issue, we integrate a crop yield reduction module and a water resources model into the MIT Integrated Global System Modeling framework, an integrated assessment model linking a global economic model to an Earth system model. We assess the effects of climate and socioeconomic changes on water availability for irrigation in the U.S. as well as subsequent impacts on crop yields by 2050, while accounting for climatemore » change projection uncertainty. We find that climate and socioeconomic changes will increase water shortages and strongly reduce irrigated yields for specific crops (i.e., cotton and forage), or in specific regions (i.e., the Southwest) where irrigation is not sustainable. Crop modeling studies that do not represent changes in irrigation availability can thus be misleading. Yet, since the most water-stressed basins represent a relatively small share of U.S. irrigated areas, the overall reduction in U.S. crop yields is small. The response of crop yields to climate change and water stress also suggests that some level of adaptation will be feasible, like relocating croplands to regions with sustainable irrigation or switching to less irrigation intensive crops. Finally, additional simulations show that greenhouse gas (GHG) mitigation can alleviate the effect of water stress on irrigated crop yields, enough to offset the reduced CO 2 fertilization effect compared to an unconstrained GHG emission scenario.« less

  7. Is current irrigation sustainable in the United States? An integrated assessment of climate change impact on water resources and irrigated crop yields

    NASA Astrophysics Data System (ADS)

    Blanc, Elodie; Caron, Justin; Fant, Charles; Monier, Erwan

    2017-08-01

    While climate change impacts on crop yields has been extensively studied, estimating the impact of water shortages on irrigated crop yields is challenging because the water resources management system is complex. To investigate this issue, we integrate a crop yield reduction module and a water resources model into the MIT Integrated Global System Modeling framework, an integrated assessment model linking a global economic model to an Earth system model. We assess the effects of climate and socioeconomic changes on water availability for irrigation in the U.S. as well as subsequent impacts on crop yields by 2050, while accounting for climate change projection uncertainty. We find that climate and socioeconomic changes will increase water shortages and strongly reduce irrigated yields for specific crops (i.e., cotton and forage), or in specific regions (i.e., the Southwest) where irrigation is not sustainable. Crop modeling studies that do not represent changes in irrigation availability can thus be misleading. Yet, since the most water-stressed basins represent a relatively small share of U.S. irrigated areas, the overall reduction in U.S. crop yields is small. The response of crop yields to climate change and water stress also suggests that some level of adaptation will be feasible, like relocating croplands to regions with sustainable irrigation or switching to less irrigation intensive crops. Finally, additional simulations show that greenhouse gas (GHG) mitigation can alleviate the effect of water stress on irrigated crop yields, enough to offset the reduced CO2 fertilization effect compared to an unconstrained GHG emission scenario.

  8. Is current irrigation sustainable in the United States? An integrated assessment of climate change impact on water resources and irrigated crop yields.

    PubMed

    Blanc, Elodie; Caron, Justin; Fant, Charles; Monier, Erwan

    2017-08-01

    While climate change impacts on crop yields has been extensively studied, estimating the impact of water shortages on irrigated crop yields is challenging because the water resources management system is complex. To investigate this issue, we integrate a crop yield reduction module and a water resources model into the MIT Integrated Global System Modeling framework, an integrated assessment model linking a global economic model to an Earth system model. We assess the effects of climate and socioeconomic changes on water availability for irrigation in the U.S. as well as subsequent impacts on crop yields by 2050, while accounting for climate change projection uncertainty. We find that climate and socioeconomic changes will increase water shortages and strongly reduce irrigated yields for specific crops (i.e., cotton and forage), or in specific regions (i.e., the Southwest) where irrigation is not sustainable. Crop modeling studies that do not represent changes in irrigation availability can thus be misleading. Yet, since the most water-stressed basins represent a relatively small share of U.S. irrigated areas, the overall reduction in U.S. crop yields is small. The response of crop yields to climate change and water stress also suggests that some level of adaptation will be feasible, like relocating croplands to regions with sustainable irrigation or switching to less irrigation intensive crops. Finally, additional simulations show that greenhouse gas (GHG) mitigation can alleviate the effect of water stress on irrigated crop yields, enough to offset the reduced CO 2 fertilization effect compared to an unconstrained GHG emission scenario.

  9. Is current irrigation sustainable in the United States? An integrated assessment of climate change impact on water resources and irrigated crop yields

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Blanc, Elodie; Caron, Justin; Fant, Charles

    While climate change impacts on crop yields has been extensively studied, estimating the impact of water shortages on irrigated crop yields is challenging because the water resources management system is complex. To investigate this issue, we integrate a crop yield reduction module and a water resources model into the MIT Integrated Global System Modeling framework, an integrated assessment model linking a global economic model to an Earth system model. We assess the effects of climate and socioeconomic changes on water availability for irrigation in the U.S. as well as subsequent impacts on crop yields by 2050, while accounting for climatemore » change projection uncertainty. We find that climate and socioeconomic changes will increase water shortages and strongly reduce irrigated yields for specific crops (i.e., cotton and forage), or in specific regions (i.e., the Southwest) where irrigation is not sustainable. Crop modeling studies that do not represent changes in irrigation availability can thus be misleading. Yet, since the most water-stressed basins represent a relatively small share of U.S. irrigated areas, the overall reduction in U.S. crop yields is small. The response of crop yields to climate change and water stress also suggests that some level of adaptation will be feasible, like relocating croplands to regions with sustainable irrigation or switching to less irrigation intensive crops. Finally, additional simulations show that greenhouse gas (GHG) mitigation can alleviate the effect of water stress on irrigated crop yields, enough to offset the reduced CO 2 fertilization effect compared to an unconstrained GHG emission scenario.« less

  10. Functional group diversity of bee pollinators increases crop yield

    PubMed Central

    Hoehn, Patrick; Tscharntke, Teja; Tylianakis, Jason M; Steffan-Dewenter, Ingolf

    2008-01-01

    Niche complementarity is a commonly invoked mechanism underlying the positive relationship between biodiversity and ecosystem functioning, but little empirical evidence exists for complementarity among pollinator species. This study related differences in three functional traits of pollinating bees (flower height preference, daily time of flower visitation and within-flower behaviour) to the seed set of the obligate cross-pollinated pumpkin Cucurbita moschata Duch. ex Poir. across a land-use intensity gradient from tropical rainforest and agroforests to grassland in Indonesia. Bee richness and abundance changed with habitat variables and we used this natural variation to test whether complementary resource use by the diverse pollinator community enhanced final yield. We found that pollinator diversity, but not abundance, was positively related to seed set of pumpkins. Bees showed species-specific spatial and temporal variation in flower visitation traits and within-flower behaviour, allowing for classification into functional guilds. Diversity of functional groups explained even more of the variance in seed set (r2=45%) than did species richness (r2=32%) highlighting the role of functional complementarity. Even though we do not provide experimental, but rather correlative evidence, we can link spatial and temporal complementarity in highly diverse pollinator communities to pollination success in the field, leading to enhanced crop yield without any managed honeybees. PMID:18595841

  11. Beneficial effect of fluoxetine treatment aganist psychological stress is mediated by increasing BDNF expression in selected brain areas

    PubMed Central

    Li, Gongying; Jing, Ping; Liu, Zhidong; Li, Zhiruo; Ma, Hongxia; Tu, Wenzhen; Zhang, Wei; Zhuo, Chuanjun

    2017-01-01

    SSRI antidepressant fluoxetine is widely used to treat psychological stress related disorders, however the underlying working mechanisms is not fully understood, as SSRIs can rapidly increase the extracellular serotonin levels but it normally takes weeks to reveal their therapeutic effect in the stress-related psychological disorders. Our previous study demonstrated that purely psychological stress without any physic stimuli induces a biphasic change in the expression of brain-derived neurotrophic factor (BDNF), which immediately decrease and then gradually increase after the stress; and that the latter BDNF increase in response to the psychological stress involves the activation of serotonin system. To investigate the role of BDNF in the fluoxetine treatment for stress-related psychological disorders, we examined the mRNA and protein levels of BDNF in the brain of Sprague-Dawley (SD) rats, which were pretreated with fluoxetine at 10 mg/kg or vehicle solution for 14 days, over 24 hour after an acute psychological stress exposure. In situ hybridization and immunohistochemistry were performed to detect the expression of BDNF at different time points in various brain regions after the psychological stress. We found that fluoxetine treatment completely blocked the BDNF decrease induced by the psychological stress, and also enhanced the gradual increase in the expression of BDNF in most of the brain regions except VTA after the psychological stress. The results suggest that the enhancement in BDNF levels induced by chronic fluoxetine treatment mediates the therapeutic effect against psychological stress. PMID:29050222

  12. Suppression of Arabidopsis RING E3 ubiquitin ligase AtATL78 increases tolerance to cold stress and decreases tolerance to drought stress.

    PubMed

    Kim, Soo Jin; Kim, Woo Taek

    2013-08-19

    AtATL78 is an Arabidopsis RING E3 ubiquitin ligase. RT-PCR and promoter-GUS assays revealed that AtATL78 was up-regulated by cold stress and down-regulated by drought. AtATL78 was localized at the plasma-membrane. Suppression of AtATL78 increased tolerance to cold stress but decreased tolerance to drought. Our data suggests that AtATL78 is a negative regulator of cold stress response and a positive regulator of drought stress response in Arabidopsis. These results further suggest that AtATL78 plays opposing roles in cold and drought stress responses. Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

  13. Palmitic acid feeding increases ceramide supply in association with increased milk yield, circulating nonesterified fatty acids, and adipose tissue responsiveness to a glucose challenge.

    PubMed

    Rico, J E; Mathews, A T; Lovett, J; Haughey, N J; McFadden, J W

    2016-11-01

    Reduced insulin action is a key adaptation that facilitates glucose partitioning to the mammary gland for milk synthesis and enhances adipose tissue lipolysis during early lactation. The progressive recovery of insulin sensitivity as cows advance toward late lactation is accompanied by reductions in circulating nonesterified fatty acids (NEFA) and milk yield. Because palmitic acid can promote insulin resistance in monogastrics through sphingolipid ceramide-dependent mechanisms, palmitic acid (C16:0) feeding may enhance milk production by restoring homeorhetic responses. We hypothesized that feeding C16:0 to mid-lactation cows would enhance ceramide supply and ceramide would be positively associated with milk yield. Twenty multiparous mid-lactation Holstein cows were enrolled in a study consisting of a 5-d covariate, 49-d treatment, and 14-d posttreatment period. All cows were randomly assigned to a sorghum silage-based diet containing no supplemental fat (control; n=10; 138±45 d in milk) or C16:0 at 4% of ration dry matter (PALM; 98% C16:0; n=10; 136±44 d in milk). Blood and milk were collected at routine intervals. Liver and skeletal muscle tissue were biopsied at d 47 of treatment. Intravenous glucose tolerance tests (300mg/kg of body weight) were performed at d -1, 24, and 49 relative to start of treatment. The plasma and tissue concentrations of ceramide and glycosylated ceramide were determined using liquid chromatography coupled with tandem mass spectrometry. Data were analyzed as repeated measures using a mixed model with fixed effects of treatment and time, and milk yield served as a covariate. The PALM treatment increased milk yield, energy-corrected milk, and milk fat yield. The most abundant plasma and tissue sphingolipids detected were C24:0-ceramide, C24:0-monohexosylceramide (GlcCer), and C16:0-lactosylceramide. Plasma concentrations of total ceramide and GlcCer decreased as lactation advanced, and ceramide and GlcCer were elevated in cows fed PALM

  14. The important but weakening maize yield benefit of grain filling prolongation in the US Midwest.

    PubMed

    Zhu, Peng; Jin, Zhenong; Zhuang, Qianlai; Ciais, Philippe; Bernacchi, Carl; Wang, Xuhui; Makowski, David; Lobell, David

    2018-06-14

    A better understanding of recent crop yield trends is necessary for improving the yield and maintaining food security. Several possible mechanisms have been investigated recently in order to explain the steady growth in maize yield over the US Corn-Belt, but a substantial fraction of the increasing trend remains elusive. In this study, trends in grain filling period (GFP) were identified and their relations with maize yield increase were further analyzed. By using satellite data from 2000 to 2015, an average lengthening of GFP of 0.37 days per year was found over the region, which probably results from variety renewal. Statistical analysis suggests that longer GFP accounted for roughly one-quarter (23%) of the yield increase trend by promoting kernel dry matter accumulation, yet had less yield benefit in hotter counties. Both official survey data and crop model simulations estimated a similar contribution of GFP trend to yield. If growing degree days that determines the GFP continues to prolong at the current rate for the next 50 years, yield reduction will be lessened with 25% and 18% longer GFP under Representative Concentration Pathway 2.6 (RCP 2.6) and RCP 6.0, respectively. However, this level of progress is insufficient to offset yield losses in future climates, because drought and heat stress during the GFP will become more prevalent and severe. This study highlights the need to devise multiple effective adaptation strategies to withstand the upcoming challenges in food security. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  15. The metabolic costs of improving ethanol yield by reducing glycerol formation capacity under anaerobic conditions in Saccharomyces cerevisiae

    PubMed Central

    2013-01-01

    Background Finely regulating the carbon flux through the glycerol pathway by regulating the expression of the rate controlling enzyme, glycerol-3-phosphate dehydrogenase (GPDH), has been a promising approach to redirect carbon from glycerol to ethanol and thereby increasing the ethanol yield in ethanol production. Here, strains engineered in the promoter of GPD1 and deleted in GPD2 were used to investigate the possibility of reducing glycerol production of Saccharomyces cerevisiae without jeopardising its ability to cope with process stress during ethanol production. For this purpose, the mutant strains TEFmut7 and TEFmut2 with different GPD1 residual expression were studied in Very High Ethanol Performance (VHEP) fed-batch process under anaerobic conditions. Results Both strains showed a drastic reduction of the glycerol yield by 44 and 61% while the ethanol yield improved by 2 and 7% respectively. TEFmut2 strain showing the highest ethanol yield was accompanied by a 28% reduction of the biomass yield. The modulation of the glycerol formation led to profound redox and energetic changes resulting in a reduction of the ATP yield (YATP) and a modulation of the production of organic acids (acetate, pyruvate and succinate). Those metabolic rearrangements resulted in a loss of ethanol and stress tolerance of the mutants, contrarily to what was previously observed under aerobiosis. Conclusions This work demonstrates the potential of fine-tuned pathway engineering, particularly when a compromise has to be found between high product yield on one hand and acceptable growth, productivity and stress resistance on the other hand. Previous study showed that, contrarily to anaerobiosis, the resulting gain in ethanol yield was accompanied with no loss of ethanol tolerance under aerobiosis. Moreover those mutants were still able to produce up to 90 gl-1 ethanol in an anaerobic SSF process. Fine tuning metabolic strategy may then open encouraging possibilities for further

  16. A natural variant of NAL1, selected in high-yield rice breeding programs, pleiotropically increases photosynthesis rate.

    PubMed

    Takai, Toshiyuki; Adachi, Shunsuke; Taguchi-Shiobara, Fumio; Sanoh-Arai, Yumiko; Iwasawa, Norio; Yoshinaga, Satoshi; Hirose, Sakiko; Taniguchi, Yojiro; Yamanouchi, Utako; Wu, Jianzhong; Matsumoto, Takashi; Sugimoto, Kazuhiko; Kondo, Katsuhiko; Ikka, Takashi; Ando, Tsuyu; Kono, Izumi; Ito, Sachie; Shomura, Ayahiko; Ookawa, Taiichiro; Hirasawa, Tadashi; Yano, Masahiro; Kondo, Motohiko; Yamamoto, Toshio

    2013-01-01

    Improvement of leaf photosynthesis is an important strategy for greater crop productivity. Here we show that the quantitative trait locus GPS (GREEN FOR PHOTOSYNTHESIS) in rice (Oryza sativa L.) controls photosynthesis rate by regulating carboxylation efficiency. Map-based cloning revealed that GPS is identical to NAL1 (NARROW LEAF1), a gene previously reported to control lateral leaf growth. The high-photosynthesis allele of GPS was found to be a partial loss-of-function allele of NAL1. This allele increased mesophyll cell number between vascular bundles, which led to thickened leaves, and it pleiotropically enhanced photosynthesis rate without the detrimental side effects observed in previously identified nal1 mutants, such as dwarf plant stature. Furthermore, pedigree analysis suggested that rice breeders have repeatedly selected the high-photosynthesis allele in high-yield breeding programs. The identification and utilization of NAL1 (GPS) can enhance future high-yield breeding and provides a new strategy for increasing rice productivity.

  17. A natural variant of NAL1, selected in high-yield rice breeding programs, pleiotropically increases photosynthesis rate

    PubMed Central

    Takai, Toshiyuki; Adachi, Shunsuke; Taguchi-Shiobara, Fumio; Sanoh-Arai, Yumiko; Iwasawa, Norio; Yoshinaga, Satoshi; Hirose, Sakiko; Taniguchi, Yojiro; Yamanouchi, Utako; Wu, Jianzhong; Matsumoto, Takashi; Sugimoto, Kazuhiko; Kondo, Katsuhiko; Ikka, Takashi; Ando, Tsuyu; Kono, Izumi; Ito, Sachie; Shomura, Ayahiko; Ookawa, Taiichiro; Hirasawa, Tadashi; Yano, Masahiro; Kondo, Motohiko; Yamamoto, Toshio

    2013-01-01

    Improvement of leaf photosynthesis is an important strategy for greater crop productivity. Here we show that the quantitative trait locus GPS (GREEN FOR PHOTOSYNTHESIS) in rice (Oryza sativa L.) controls photosynthesis rate by regulating carboxylation efficiency. Map-based cloning revealed that GPS is identical to NAL1 (NARROW LEAF1), a gene previously reported to control lateral leaf growth. The high-photosynthesis allele of GPS was found to be a partial loss-of-function allele of NAL1. This allele increased mesophyll cell number between vascular bundles, which led to thickened leaves, and it pleiotropically enhanced photosynthesis rate without the detrimental side effects observed in previously identified nal1 mutants, such as dwarf plant stature. Furthermore, pedigree analysis suggested that rice breeders have repeatedly selected the high-photosynthesis allele in high-yield breeding programs. The identification and utilization of NAL1 (GPS) can enhance future high-yield breeding and provides a new strategy for increasing rice productivity. PMID:23985993

  18. Application of Bioorganic Fertilizer Significantly Increased Apple Yields and Shaped Bacterial Community Structure in Orchard Soil.

    PubMed

    Wang, Lei; Li, Jing; Yang, Fang; E, Yaoyao; Raza, Waseem; Huang, Qiwei; Shen, Qirong

    2017-02-01

    Application of bioorganic fertilizers has been reported to improve crop yields and change soil bacterial community structure; however, little work has been done in apple orchard soils where the biological properties of the soils are being degraded due to long-term application of chemical fertilizers. In this study, we used Illumina-based sequencing approach to characterize the bacterial community in the 0-60-cm soil profile under different fertilizer regimes in the Loess Plateau. The experiment includes three treatments: (1) control without fertilization (CK); (2) application of chemical fertilizer (CF); and (3) application of bioorganic fertilizer and organic-inorganic mixed fertilizer (BOF). The results showed that the treatment BOF increased the apple yields by 114 and 67 % compared to the CK and CF treatments, respectively. The treatment BOF also increased the soil organic matter (SOM) by 22 and 16 % compared to the CK and CF treatments, respectively. The Illumina-based sequencing showed that Acidobacteria and Proteobacteria were the predominant phyla and Alphaproteobacteria and Gammaproteobacteria were the most abundant classes in the soil profile. The bacterial richness for ACE was increased after the addition of BOF. Compared to CK and CF treatments, BOF-treated soil revealed higher abundance of Proteobacteria, Alphaproteobacteria and Gammaproteobacteria, Rhizobiales, and Xanthomonadales while Acidobacteria, Gp7, Gp17, and Sphaerobacter were found in lower abundance throughout the soil profile. Bacterial community structure varied with soil depth under different fertilizer treatments, e.g., the bacterial richness, diversity, and the relative abundance of Verruccomicrobia, Candidatus Brocadiales, and Skermanella were decreased with the soil depth in all three treatments. Permutational multivariate analysis showed that the fertilizer regime was the major factor than soil depth in the variations of the bacterial community composition. Two groups, Lysobacter

  19. Methods for Alleviating Stress and Increasing Resilience in the Midwifery Community: A Scoping Review of the Literature.

    PubMed

    Wright, Erin M; Matthai, Maude Theo; Warren, Nicole

    2017-11-01

    Work-related stress and exposure to traumatic birth have deleterious impacts on midwifery practice, the midwife's physiologic well-being, and the midwifery workforce. This is a global phenomenon, and the specific sources of this stress vary dependent on practice setting. This scoping review aims to determine which, if any, modalities help to reduce stress and increase resilience among a population of midwives. A scoping review of the literature published between January 2011 and September 2016 using PubMed, CINAHL, Embase, PsycINFO, and Cochrane databases was performed. Of the initial 796 reviewed records, 6 met inclusion criteria. Three of the 6 included studies were quantitative in nature, 2 were qualitative, and one used mixed methods. Countries where studies were conducted include Uganda, Iran, the United Kingdom, Israel, and Australia. Three of the studies used interventions for stress reduction and increased coping. Two of these 3 used a mindfulness-based stress reduction program resulting in improved stress levels and coping skills. In each study, midwives express a desire for work-based programs and support from colleagues and employers for increasing coping abilities. These studies focused on stress reduction and/or increasing resilience. While modalities such as mindfulness-based stress reduction show promise, further studies with a cohort of midwives should be conducted. These studies should include interventions aimed at addressing the needs of midwives to improve psychological outcomes related to employment-related stress on a global scale and specific to each health care context. © 2017 by the American College of Nurse-Midwives.

  20. Ropivacaine and Bupivacaine prevent increased pain sensitivity without altering neuroimmune activation following repeated social defeat stress.

    PubMed

    Sawicki, Caroline M; Kim, January K; Weber, Michael D; Jarrett, Brant L; Godbout, Jonathan P; Sheridan, John F; Humeidan, Michelle

    2018-03-01

    Mounting evidence indicates that stress influences the experience of pain. Exposure to psychosocial stress disrupts bi-directional communication pathways between the central nervous system and peripheral immune system, and can exacerbate the frequency and severity of pain experienced by stressed subjects. Repeated social defeat (RSD) is a murine model of psychosocial stress that recapitulates the immune and behavioral responses to stress observed in humans, including activation of stress-reactive neurocircuitry and increased pro-inflammatory cytokine production. It is unclear, however, how these stress-induced neuroimmune responses contribute to increased pain sensitivity in mice exposed to RSD. Here we used a technique of regional analgesia with local anesthetics in mice to block the development of mechanical allodynia during RSD. We next investigated the degree to which pain blockade altered stress-induced neuroimmune activation and depressive-like behavior. Following development of a mouse model of regional analgesia with discrete sensory blockade over the dorsal-caudal aspect of the spine, C57BL/6 mice were divided into experimental groups and treated with Ropivacaine (0.08%), Liposomal Bupivacaine (0.08%), or Vehicle (0.9% NaCl) prior to exposure to stress. This specific region was selected for analgesia because it is the most frequent location for aggression-associated pain due to biting during RSD. Mechanical allodynia was assessed 12 h after the first, third, and sixth day of RSD after resolution of the sensory blockade. In a separate experiment, social avoidance behavior was determined after the sixth day of RSD. Blood, bone marrow, brain, and spinal cord were collected for immunological analyses after the last day of RSD in both experiments following behavioral assessments. RSD increased mechanical allodynia in an exposure-dependent manner that persisted for at least one week following cessation of the stressor. Mice treated with either Ropivacaine or