Sample records for yoyu aen mekki

  1. Time-Dependent Regulation of Apoptosis by AEN and BAX in Response to 2-Aminoanthracene Dietary Consumption

    PubMed Central

    Gato, Worlanyo Eric; McGee, Stacey R.; Hales, Dale B.; Means, Jay C.

    2014-01-01

    Background/Objective: The modulation of the toxic effects of 2-aminoanthracene (2AA) on the liver by apoptosis was investigated. Fisher-344 (F344) rats were exposed to various concentrations of 2AA for 14 and 28 days. The arylamine 2AA is an aromatic hydrocarbon employed in manufacturing chemicals, dyes, inks, and it is also a curing agent in epoxy resins and polyurethanes. 2AA has been detected in tobacco smoke and cooked foods. Methods: Analysis of total messenger ribonucleic acid (mRNA) extracts from liver for apoptosis-related gene expression changes in apoptosis enhancing nuclease (AEN), Bcl2-associated X protein (BAX), CASP3, Jun proto-oncogene (JUN), murine double minute-2 p53 binding protein homolog (MDM2), tumor protein p53 (p53), and GAPDH genes by quantitative real-time polymerase chain reaction (qRT-PCR) was coupled with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and caspase-3 (Casp3) activity assays. Results: Specific apoptosis staining result does not seem to show significant difference between control and treated animals. This may be due to freeze-thaw artifacts observed in the liver samples. However, there appears to be a greater level of apoptosis in medium- and high-dose (MD and HD) 2AA treated animals. Analyses of apoptosis-related genes seem to show AEN and BAX as the main targets in the induction of apoptosis in response to 2AA exposure, though p53, MDM2, and JUN may play supporting roles. Conclusion: Dose-dependent increases in mRNA expression were observed in all genes except Casp3. BAX was very highly expressed in the HD rats belonging to the 2-week exposure group. This trend was not observed in the animals treated for 4 weeks. Instead, AEN was rather very highly expressed in the liver of the MD animals that were treated with 2AA for 28 days. PMID:24748736

  2. Fuzzy Logic for Determination of Crack Severity in Defense Applications

    DTIC Science & Technology

    2010-03-31

    FUZZY LOGIC FOR DETERMINATION OF CRACK SEVERITY IN DEFENSE APPLICATIONS Vijay Sharma , Harpreet Singh, Arati M. Dixit, Ahmed Mekki Department of... Vijay Sharma ; Harpreet Singh; Arati M. Dixit; Ahmed Mekki 5d. PROJECT NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S

  3. Narrowing the agronomic yield gap with improved nitrogen use efficiency: a modeling approach.

    PubMed

    Ahrens, T D; Lobell, D B; Ortiz-Monasterio, J I; Li, Y; Matson, P A

    2010-01-01

    Improving nitrogen use efficiency (NUE) in the major cereals is critical for more sustainable nitrogen use in high-input agriculture, but our understanding of the potential for NUE improvement is limited by a paucity of reliable on-farm measurements. Limited on-farm data suggest that agronomic NUE (AE(N)) is lower and more variable than data from trials conducted at research stations, on which much of our understanding of AE(N) has been built. The purpose of this study was to determine the magnitude and causes of variability in AE(N) across an agricultural region, which we refer to as the achievement distribution of AE(N). The distribution of simulated AE(N) in 80 farmers' fields in an irrigated wheat system in the Yaqui Valley, Mexico, was compared with trials at a local research center (International Wheat and Maize Improvement Center; CIMMYT). An agroecosystem simulation model WNMM was used to understand factors controlling yield, AE(N), gaseous N emissions, and nitrate leaching in the region. Simulated AE(N) in the Yaqui Valley was highly variable, and mean on-farm AE(N) was 44% lower than trials with similar fertilization rates at CIMMYT. Variability in residual N supply was the most important factor determining simulated AE(N). Better split applications of N fertilizer led to almost a doubling of AE(N), increased profit, and reduced N pollution, and even larger improvements were possible with technologies that allow for direct measurement of soil N supply and plant N demand, such as site-specific nitrogen management.

  4. Neuroprotection and mechanisms of atractylenolide III in preventing learning and memory impairment induced by chronic high-dose homocysteine administration in rats.

    PubMed

    Zhao, H; Ji, Z-H; Liu, C; Yu, X-Y

    2015-04-02

    Studies demonstrated that chronic high-dose homocysteine administration induced learning and memory impairment in animals. Atractylenolide III (Aen-III), a neuroprotective constituent of Atractylodis macrocephalae Koidz, was isolated in our previous study. In this study, we investigated potential benefits of Aen-III in preventing learning and memory impairment following chronic high-dose homocysteine administration in rats. Results showed that administration of Aen-III significantly ameliorated learning and memory impairment induced by chronic high-dose homocysteine administration in rats, decreased homocysteine-induced reactive oxygen species (ROS) formation and restored homocysteine-induced decrease of phosphorylated protein kinase C expression level. Moreover, Aen-III protected primary cultured neurons from apoptotic death induced by homocysteine treatment. This study provides the first evidence for the neuroprotective effect of Aen-III in preventing learning and impairment induced by chronic administration of homocysteine. Aen-III may have therapeutic potential in treating homocysteine-mediated cognitive impairment and neuronal injury. Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.

  5. Acute esophageal necrosis caused by alcohol abuse

    PubMed Central

    Endo, Tetsu; Sakamoto, Juichi; Sato, Ken; Takimoto, Miyako; Shimaya, Koji; Mikami, Tatsuya; Munakata, Akihiro; Shimoyama, Tadashi; Fukuda, Shinsaku

    2005-01-01

    Acute esophageal necrosis (AEN) is extremely rare and the pathogenesis of this is still unknown. We report a case of AEN caused by alcohol abuse. In our case, the main pathogenesis could be accounted for low systemic perfusion caused by severe alcoholic lactic acidosis. After the healing of AEN, balloon dilatation was effective to manage the stricture. PMID:16222758

  6. UNMYELINATED FIBERS OF THE ANTERIOR ETHMOIDAL NERVE IN THE RAT CO-LOCALIZE WITH NEURONS IN THE MEDULLARY DORSAL HORN AND VENTROLATERAL MEDULLA ACTIVATED BY NASAL STIMULATION

    PubMed Central

    Hollandsworth, Michael P.; DiNovo, Karyn M.; McCulloch, Paul F.

    2009-01-01

    The anterior ethmoidal nerve (AEN) innervates the nasal passages and external nares, and serves as the afferent limb of the nasopharyngeal and diving responses. However, although 65% of the AEN is composed of unmyelinated fibers, it has not been determined whether this afferent signal is carried by unmyelinated or myelinated fibers. We used the transganglionic tracers WGA-HRP, IB4-HRP, and CTB-HRP to trace the central projections of the AEN of the rat. Interpretation of the labeling patterns suggests that AEN unmyelinated fibers project primarily to the ventral tip of the ipsilateral medullary dorsal horn (MDH) at the level of the area postrema. Other unmyelinated projections were to the ventral paratrigeminal nucleus and ventrolateral medulla, specifically the Bötzinger and RVLM/C1 regions. Myelinated AEN fibers projected to the ventral paratrigeminal and mesencephalic trigeminal nuclei. Stimulating the nasal passages of urethane-anesthetized rats with ammonia vapors produced the nasopharyngeal response that included apnea, bradycardia and an increase in arterial blood pressure. Central projections of the AEN co-localized with neurons within both MDH and RVLM/C1 that were activated by nasal stimulation. Within the ventral MDH the density of AEN terminal projections positively correlated with the rostral-caudal location of activated neurons, especially at and just caudal to the obex. We conclude that unmyelinated AEN terminal projections are involved in the activation of neurons in the MDH and ventrolateral medulla that participate in the nasopharyngeal response in the rat. We also found that IB4-HRP was a much less robust tracer than WGA-HRP. PMID:19732757

  7. Acute Esophageal Necrosis: An Update

    PubMed Central

    Inayat, Faisal; Hurairah, Abu; Virk, Hafeez Ul Hassan

    2016-01-01

    Acute esophageal necrosis (AEN) or “black esophagus” is a rare clinical entity with an unclear etiology. It is diagnosed at upper gastrointestinal endoscopy with the presence of strikingly black necrotic esophagus. The treatment is primarily medical, but the prognosis is generally poor due to advanced age and comorbid illnesses in patients who develop AEN. Herein, we discussed the implications of poor glycemic control in regards with AEN and undertook a literature review of this rare diagnosis. PMID:27583242

  8. Acute Esophageal Necrosis Presenting With Henoch-Schönlein Purpura

    PubMed Central

    Bernstein, Gregory R.; Malik, Zubair; Schey, Ron

    2015-01-01

    A 63-year-old woman with abdominal pain and melena developed a palpable, purpuric rash and acute kidney injury. Skin and kidney biopsy confirmed Henoch-Schönlein purpura. Upper endoscopy revealed diffuse, circumferential, black-appearing mucosa of the esophagus consistent with acute esophageal necrosis (AEN), also known as black esophagus. AEN is a very rare cause of gastrointestinal hemorrhage with a high mortality risk. To our knowledge, there have been no prior reports of AEN associated with Henoch-Schonlein purpura or other vasculitis. PMID:26504868

  9. Repetitive Diving in Trained Rats Still Increases Fos Production in Brainstem Neurons after Bilateral Sectioning of the Anterior Ethmoidal Nerve

    PubMed Central

    McCulloch, Paul F.; Warren, Erik A.; DiNovo, Karyn M.

    2016-01-01

    This research was designed to investigate the role of the anterior ethmoidal nerve (AEN) during repetitive trained diving in rats, with specific attention to activation of afferent and efferent brainstem nuclei that are part of this reflexive response. The AEN innervates the nose and nasal passages and is thought to be an important component of the afferent limb of the diving response. Male Sprague-Dawley rats (N = 24) were trained to swim and dive through a 5 m underwater maze. Some rats (N = 12) had bilateral sectioning of the AEN, others a Sham surgery (N = 12). Twelve rats (6 AEN cut and 6 Sham) had 24 post-surgical dive trials over 2 h to activate brainstem neurons to produce Fos, a neuronal activation marker. Remaining rats were non-diving controls. Diving animals had significantly more Fos-positive neurons than non-diving animals in the caudal pressor area, ventral medullary dorsal horn, ventral paratrigeminal nucleus, nucleus tractus solitarius, rostral ventrolateral medulla, Raphe nuclei, A5, Locus Coeruleus, and Kölliker-Fuse area. There were no significant differences in brainstem Fos labeling in rats diving with and without intact AENs. Thus, the AENs are not required for initiation of the diving response. Other nerve(s) that innervate the nose and nasal passages, and/or suprabulbar activation of brainstem neurons, may be responsible for the pattern of neuronal activation observed during repetitive trained diving in rats. These results help define the central neuronal circuitry of the mammalian diving response. PMID:27148082

  10. Acute esophageal necrosis and low-flow state

    PubMed Central

    Burtally, Ahmad; Gregoire, Philippe

    2007-01-01

    Acute esophageal necrosis (AEN), also called black esophagus, is quite exceptional. Endoscopic findings show circumferential black discolouration of the esophagus with or without exudates. The etiology of AEN is presently unknown and is assumed to be multifactorial. Distal esophageal involvement with proximal extension ending sharply at the gastroesophageal junction is the most common presentation. The present case report describes the clinical and endoscopic evolution of black esophagus observed in a patient with significant peripheral vascular disease, who was presented to the intensive care unit at the Hopital Saint-Francois d’Assise (Quebec City, Quebec). Through an extensive review of the literature, common underlying clinical conditions of patients diagnosed with AEN have been identified. PMID:17431514

  11. Bilateral sectioning of the anterior ethmoidal nerves does not eliminate the diving response in voluntarily diving rats

    PubMed Central

    Chotiyanonta, Jill S; DiNovo, Karyn M; McCulloch, Paul F

    2013-01-01

    The diving response is characterized by bradycardia, apnea, and increased peripheral resistance. This reflex response is initiated by immersing the nose in water. Because the anterior ethmoidal nerve (AEN) innervates the nose, our hypothesis was that intact AENs are essential for initiating the diving response in voluntarily diving rats. Heart rate (HR) and arterial blood pressure (BPa) were monitored using implanted biotransmitters. Sprague-Dawley rats were trained to voluntarily swim 5 m underwater. During diving, HR decreased from 480 ± 15 to 99 ± 5 bpm and BPa increased from 136 ± 2 to 187 ± 3 mmHg. Experimental rats (N = 9) then received bilateral AEN sectioning, while Sham rats (N = 8) did not. During diving in Experimental rats 7 days after AEN surgery, HR decreased from 478 ± 13 to 76 ± 4 bpm and BPa increased from 134 ± 3 to 186 ± 4 mmHg. Responses were similar in Sham rats. Then, during nasal stimulation with ammonia vapors in urethane-anesthetized Experimental rats, HR decreased from 368 ± 7 to 83 ± 4 bpm, and BPa increased from 126 ± 7 to 175 ± 4 mmHg. Responses were similar in Sham rats. Thus, 1 week after being sectioned the AENs are not essential for initiating a full cardiorespiratory response during both voluntary diving and nasal stimulation. We conclude that other nerve(s) innervating the nose are able to provide an afferent signal sufficient to initiate the diving response, although neuronal plasticity within the medullary dorsal horn may be necessary for this to occur. PMID:24400143

  12. A new class of organic nitrates: investigations on bioactivation, tolerance and cross-tolerance phenomena.

    PubMed

    Schuhmacher, S; Schulz, E; Oelze, M; König, A; Roegler, C; Lange, K; Sydow, L; Kawamoto, T; Wenzel, P; Münzel, T; Lehmann, J; Daiber, A

    2009-09-01

    The chronic use of organic nitrates is limited by serious side effects including oxidative stress, nitrate tolerance and/or endothelial dysfunction. The side effects and potency of nitroglycerine depend on mitochondrial aldehyde dehydrogenase (ALDH-2). We sought to determine whether this concept can be extended to a new class of organic nitrates with amino moieties (aminoalkyl nitrates). Vasodilator potency of the organic nitrates, in vitro tolerance and in vivo tolerance (after continuous infusion for 3 days) were assessed in wild-type and ALDH-2 knockout mice by isometric tension studies. Mitochondrial oxidative stress was analysed by L-012-dependent chemiluminescence and protein tyrosine nitration. Aminoethyl nitrate (AEN) showed an almost similar potency to glyceryl trinitrate (GTN), even though it is only a mononitrate. AEN-dependent vasodilatation was mediated by cGMP and nitric oxide. In contrast to triethanolamine trinitrate (TEAN) and GTN, AEN bioactivation did not depend on ALDH-2 and caused no in vitro tolerance. In vivo treatment with TEAN and GTN, but not with AEN, induced cross-tolerance to acetylcholine (ACh)-dependent and GTN-dependent relaxation. Although all nitrates tested induced tolerance to themselves, only TEAN and GTN significantly increased mitochondrial oxidative stress in vitro and in vivo. The present results demonstrate that not all high potency nitrates are bioactivated by ALDH-2 and that high potency of a given nitrate is not necessarily associated with induction of oxidative stress or nitrate tolerance. Obviously, there are distinct pathways for bioactivation of organic nitrates, which for AEN may involve xanthine oxidoreductase rather than P450 enzymes.

  13. Acute Esophageal Necrosis: A Case of Black Esophagus Associated with Bismuth Subsalicylate Ingestion

    PubMed Central

    Mankal, Pavan; Judeh, Hani; Kim, Sang

    2014-01-01

    We present a case of acute esophageal necrosis (AEN) likely caused by chronic use of bismuth subsalicylate, an active ingredient in over-the-counter Pepto-Bismol®, which contains 220 g of salicylic acid in each 30 mL quantity. While aspirin is known to cause gastritis and gastric ulcers, this is the first case, to our knowledge, reporting AEN after chronic bismuth subsalicylate use. PMID:26157851

  14. A new class of organic nitrates: investigations on bioactivation, tolerance and cross-tolerance phenomena

    PubMed Central

    Schuhmacher, S; Schulz, E; Oelze, M; König, A; Roegler, C; Lange, K; Sydow, L; Kawamoto, T; Wenzel, P; Münzel, T; Lehmann, J; Daiber, A

    2009-01-01

    Background and purpose: The chronic use of organic nitrates is limited by serious side effects including oxidative stress, nitrate tolerance and/or endothelial dysfunction. The side effects and potency of nitroglycerine depend on mitochondrial aldehyde dehydrogenase (ALDH-2). We sought to determine whether this concept can be extended to a new class of organic nitrates with amino moieties (aminoalkyl nitrates). Experimental approach: Vasodilator potency of the organic nitrates, in vitro tolerance and in vivo tolerance (after continuous infusion for 3 days) were assessed in wild-type and ALDH-2 knockout mice by isometric tension studies. Mitochondrial oxidative stress was analysed by L-012-dependent chemiluminescence and protein tyrosine nitration. Key results: Aminoethyl nitrate (AEN) showed an almost similar potency to glyceryl trinitrate (GTN), even though it is only a mononitrate. AEN-dependent vasodilatation was mediated by cGMP and nitric oxide. In contrast to triethanolamine trinitrate (TEAN) and GTN, AEN bioactivation did not depend on ALDH-2 and caused no in vitro tolerance. In vivo treatment with TEAN and GTN, but not with AEN, induced cross-tolerance to acetylcholine (ACh)-dependent and GTN-dependent relaxation. Although all nitrates tested induced tolerance to themselves, only TEAN and GTN significantly increased mitochondrial oxidative stress in vitro and in vivo. Conclusions and implications: The present results demonstrate that not all high potency nitrates are bioactivated by ALDH-2 and that high potency of a given nitrate is not necessarily associated with induction of oxidative stress or nitrate tolerance. Obviously, there are distinct pathways for bioactivation of organic nitrates, which for AEN may involve xanthine oxidoreductase rather than P450 enzymes. PMID:19563531

  15. Time-dependent middle ear pressure changes under general anaesthesia in children: N2O-O2 mixture versus air-oxygen mixture.

    PubMed

    Apan, A; Muluk, N Bayar; Güler, S; Budak, B

    2013-01-01

    The aim of this study was to investigate the effects of N2O-O2 mixture (Inspired O2 30%) on middle ear pressure (MEP) in children compared with the effects of an air-oxygen mixture (Inspired O2 50%). The study included thirty child patients who underwent general anaesthesia for different reasons, with the exception of ENT problems and ear interventions. They were randomly divided into two groups. Group 1 (15 children: 10 male and 5 female) received a N2O-O2 mixture (Inspired O2 30%); and group 2 (15 children: 10 male and 5 female) were given an air-oxygen mixture (Inspired O2 50%). MEP was measured using a portable impedance analyser before the operation (PreO),10 minutes after intubation (10AEn), 30 minutes after intubation (30AEn), 10 minutes before extubation (10BEx), 15 minutes after the operation (PO15), 30 minutes after the operation (PO30), 1 hour after the operation (PO1h) and 6 hours after the operation (PO6h). The pressure and compliance values were the same in groups 1 and 2. The pressure-time graphs for the two groups were different: in Group 2, MEP rose quickly at 10AEn and positive pressure values were seen in the middle ear. MEP then fell rapidly until the end of the surgery and lower and negative pressures (Mean -50 daPa) were observed at PO6h. In Group 1, MEP was elevated at 10AEn and positive pressure was found (but not as high as in Group 2). MEP then fell more slowly. In other words, positive pressure in the middle ear persisted longer and the middle ear was subjected to positive pressure and nitrogen over a longer period. Separate analyses were made in Groups 1 and 2 of pressure differences and of compliance values at eight measurement points using the Friedman test. Differences in pressure values were found to be statistically significant in both Group 1 (p = 0.000) and Group 2 (p = 0.000). In Group 1, all the 10AEn and 30AEn values were significantly higher than the PreO, PO30, PO1h and PO6h values. The 10BEx value was significantly higher

  16. Successful bypass operation for esophageal obstruction after acute esophageal necrosis: a case report.

    PubMed

    Sakatoku, Yayoi; Fukaya, Masahide; Miyata, Kazushi; Nagino, Masato

    2017-12-01

    Acute esophageal necrosis (AEN) is a rare clinical disorder. Esophageal stenosis or obstruction is one of severe complications, but there are a few reports about surgical treatments. In such a situation, it still remains controversial which to choose, esophagectomy or bypass operation. A 61-year-old woman was admitted to the local hospital for septic shock with diabetic ketoacidosis due to necrotizing fasciitis of the right thigh. Three days later, she had hematemesis, and gastrointestinal endoscopy revealed black mucosal coloration throughout the entire esophagus. She was diagnosed as having AEN. Her general condition improved after intensive care, debridement, and treatment with antibiotics and a proton pump inhibitor; the esophageal mucosal color recovered. However, an esophageal stricture developed after 1 month, and the patient underwent gastrostomy to remove an esophageal obstruction after 3 months. She was referred to our hospital for surgical treatment 1 year and 4 months after the occurrence of AEN because of her strong desire for oral intake. Her medical condition was poor, and she could not walk due to generalized muscle weakness. After rehabilitation for 8 months, we performed an esophageal bypass using a gastric conduit via the percutaneous route rather than esophagectomy because of her multiple severe comorbidities including walking difficulty, chronic hepatitis C, cerebrovascular disease, and chronic renal failure. Minor leakage of the esophagogastrostomy occurred and was resolved with conservative treatment. The patient began oral intake on postoperative day 34 and was discharged on day 52. Esophageal obstruction after AEN was successfully treated by esophageal bypass using a gastric conduit in a high-risk patient. Because the majority of patients with AEN have multiple severe comorbidities, assessing the medical condition of the patient adequately is important prior to choosing either an esophagectomy or bypass surgery.

  17. JPRS Report, Proliferation Issues

    DTIC Science & Technology

    1992-05-27

    6 MOROCCO Berrada on Proposed Nuclear Power Plant [ MAROC SOIR 22 Apr] 6 JPRS-TND-92-016 27 May 1992 2 CENTRAL EURASIA Proposals on...Proposed Nuclear Power Plant 92AF0789Z Casablanca MAROC SOIR in French 22 Apr 92 p 7 [Interview with Mekki Berrada Abdelhamid, nuclear engi...questions about the use of nuclear energy in our country, its growth, and the laws that regulate it. [ MAROC SOIR] Why was Sidi Boulbra chosen as

  18. The a priori SDR Estimation Techniques with Reduced Speech Distortion for Acoustic Echo and Noise Suppression

    NASA Astrophysics Data System (ADS)

    Thoonsaengngam, Rattapol; Tangsangiumvisai, Nisachon

    This paper proposes an enhanced method for estimating the a priori Signal-to-Disturbance Ratio (SDR) to be employed in the Acoustic Echo and Noise Suppression (AENS) system for full-duplex hands-free communications. The proposed a priori SDR estimation technique is modified based upon the Two-Step Noise Reduction (TSNR) algorithm to suppress the background noise while preserving speech spectral components. In addition, a practical approach to determine accurately the Echo Spectrum Variance (ESV) is presented based upon the linear relationship assumption between the power spectrum of far-end speech and acoustic echo signals. The ESV estimation technique is then employed to alleviate the acoustic echo problem. The performance of the AENS system that employs these two proposed estimation techniques is evaluated through the Echo Attenuation (EA), Noise Attenuation (NA), and two speech distortion measures. Simulation results based upon real speech signals guarantee that our improved AENS system is able to mitigate efficiently the problem of acoustic echo and background noise, while preserving the speech quality and speech intelligibility.

  19. Acute Esophageal Necrosis in an Alcoholic after Successful Resuscitation from Cardiac Arrest

    PubMed Central

    Thoguluva Chandreskar, Viveksandeep; Kahlon, Arundeep; Amzuta, Ioana

    2017-01-01

    Introduction We present a patient who presented to the ICU after successful resuscitation from cardiac arrest who was subsequently diagnosed with AEN. Case Presentation A 66-year-old female presented after cardiac arrest in which return of spontaneous circulation was achieved within 7 minutes after the initiation of CPR. She was intubated on the scene and found to have coffee ground emesis in her bathroom when found unresponsive. Due to the hemodynamically significant GI bleed, patient was started on IV proton pump inhibitor, octreotide, and levophed. Subsequent endoscopy showed diffuse severe mucosal changes characterized by blackness, erythema, friability, granularity, inflammation, and decreased vascular pattern in the middle third of the esophagus and in the lower third of the esophagus. Discussion AEN is a rare syndrome with a prevalence ranging from 0.001 to 0.2% of EGD. This patient is especially rare as this patient was female and had AEN in the middle esophagus along with lower esophagus. The pathophysiology in this patient is hypothesized that she had cardiac arrest secondary to acute upper GI hemorrhage. Subsequent low-flow state (cardiac arrest) in addition to being in the setting of severe alcohol abuse led to esophageal necrosis. PMID:28706745

  20. Oral supplementation with carbohydrate- and branched-chain amino acid-enriched nutrients improves postoperative quality of life in patients undergoing hepatic resection.

    PubMed

    Okabayashi, Takehiro; Iyoki, Miho; Sugimoto, Takeki; Kobayashi, Michiya; Hanazaki, Kazuhiro

    2011-04-01

    The long-term outcomes of branched-chain amino acid (BCAA) administration in patients undergoing hepatic resection remain unclear. The aim of this study is to assess the impact of oral supplementation with BCAA-enriched nutrients on postoperative quality of life (QOL) in patients undergoing liver resection. A prospective randomized clinical trial was conducted in 96 patients undergoing hepatic resection. Patients were randomly assigned to receive BCAA supplementation (AEN group, n = 48) or a conventional diet (control group, n = 48). Postoperative QOL and short-term outcomes were regularly and continuously evaluated in all patients using a short-form 36 (SF-36) health questionnaire and by measuring various clinical parameters. This study demonstrated a significant improvement in QOL after hepatectomy for liver neoplasm in the AEN group based on the same patients' preoperative SF-36 scores (P < 0.05). Perioperative BCAA supplementation preserved liver function and general patient health in the short term for AEN group patients compared to those not receiving the nutritional supplement. BCAA supplementation improved postoperative QOL after hepatic resection over the long term by restoring and maintaining nutritional status and whole-body kinetics. This study was registered at http://www.clinicaltrials.gov (registration number: NCT00945568).

  1. New insights into non-avian dinosaur reproduction and their evolutionary and ecological implications: linking fossil evidence to allometries of extant close relatives.

    PubMed

    Werner, Jan; Griebeler, Eva Maria

    2013-01-01

    It has been hypothesized that a high reproductive output contributes to the unique gigantism in large dinosaur taxa. In order to infer more information on dinosaur reproduction, we established allometries between body mass and different reproductive traits (egg mass, clutch mass, annual clutch mass) for extant phylogenetic brackets (birds, crocodiles and tortoises) of extinct non-avian dinosaurs. Allometries were applied to nine non-avian dinosaur taxa (theropods, hadrosaurs, and sauropodomorphs) for which fossil estimates on relevant traits are currently available. We found that the reproductive traits of most dinosaurs conformed to similar-sized or scaled-up extant reptiles or birds. The reproductive traits of theropods, which are considered more bird-like, were indeed consistent with birds, while the traits of sauropodomorphs conformed better to reptiles. Reproductive traits of hadrosaurs corresponded to both reptiles and birds. Excluding Massospondyluscarinatus, all dinosaurs studied had an intermediary egg to body mass relationship to reptiles and birds. In contrast, dinosaur clutch masses fitted with either the masses predicted from allometries of birds (theropods) or to the masses of reptiles (all other taxa). Theropods studied had probably one clutch per year. For sauropodomorphs and hadrosaurs, more than one clutch per year was predicted. Contrary to current hypotheses, large dinosaurs did not have exceptionally high annual egg numbers (AEN). Independent of the extant model, the estimated dinosaur AEN did not exceed 850 eggs (75,000 kg sauropod) for any of the taxa studied. This estimated maximum is probably an overestimation due to unrealistic assumptions. According to most AEN estimations, the dinosaurs studied laid less than 200 eggs per year. Only some AEN estimates obtained for medium to large sized sauropods were higher (200-400 eggs). Our results provide new (testable) hypotheses, especially for reproductive traits that are insufficiently documented

  2. Direct reticular projections of trigeminal sensory fibers immunoreactive to CGRP: potential monosynaptic somatoautonomic projections

    PubMed Central

    Panneton, W. Michael; Gan, Qi

    2014-01-01

    Few trigeminal sensory fibers project centrally beyond the trigeminal sensory complex, with only projections of fibers carried in its sensory anterior ethmoidal (AEN) and intraoral nerves described. Fibers of the AEN project into the brainstem reticular formation where immunoreactivity against substance P and CGRP are found. We investigated whether the source of these peptides could be from trigeminal ganglion neurons by performing unilateral rhizotomies of the trigeminal root and looking for absence of label. After an 8–14 days survival, substance P immunoreactivity in the trigeminal sensory complex was diminished, but we could not conclude that the sole source of this peptide in the lateral parabrachial area and lateral reticular formation arises from primary afferent fibers. Immunoreactivity to CGRP after rhizotomy however was greatly diminished in the trigeminal sensory complex, confirming the observations of others. Moreover, CGRP immunoreactivity was nearly eliminated in fibers in the lateral parabrachial area, the caudal ventrolateral medulla, both the peri-ambiguus and ventral parts of the rostral ventrolateral medulla, in the external formation of the nucleus ambiguus, and diminished in the caudal pressor area. The nearly complete elimination of CGRP in the lateral reticular formation after rhizotomy suggests this peptide is carried in primary afferent fibers. Moreover, the arborization of CGRP immunoreactive fibers in these areas mimics that of direct projections from the AEN. Since electrical stimulation of the AEN induces cardiorespiratory adjustments including an apnea, peripheral vasoconstriction, and bradycardia similar to those seen in the mammalian diving response, we suggest these perturbations of autonomic behavior are enhanced by direct somatic primary afferent projections to these reticular neurons. We believe this to be first description of potential direct somatoautonomic projections to brainstem neurons regulating autonomic activity. PMID

  3. New Insights into Non-Avian Dinosaur Reproduction and Their Evolutionary and Ecological Implications: Linking Fossil Evidence to Allometries of Extant Close Relatives

    PubMed Central

    Werner, Jan; Griebeler, Eva Maria

    2013-01-01

    It has been hypothesized that a high reproductive output contributes to the unique gigantism in large dinosaur taxa. In order to infer more information on dinosaur reproduction, we established allometries between body mass and different reproductive traits (egg mass, clutch mass, annual clutch mass) for extant phylogenetic brackets (birds, crocodiles and tortoises) of extinct non-avian dinosaurs. Allometries were applied to nine non-avian dinosaur taxa (theropods, hadrosaurs, and sauropodomorphs) for which fossil estimates on relevant traits are currently available. We found that the reproductive traits of most dinosaurs conformed to similar-sized or scaled-up extant reptiles or birds. The reproductive traits of theropods, which are considered more bird-like, were indeed consistent with birds, while the traits of sauropodomorphs conformed better to reptiles. Reproductive traits of hadrosaurs corresponded to both reptiles and birds. Excluding Massospondylus carinatus , all dinosaurs studied had an intermediary egg to body mass relationship to reptiles and birds. In contrast, dinosaur clutch masses fitted with either the masses predicted from allometries of birds (theropods) or to the masses of reptiles (all other taxa). Theropods studied had probably one clutch per year. For sauropodomorphs and hadrosaurs, more than one clutch per year was predicted. Contrary to current hypotheses, large dinosaurs did not have exceptionally high annual egg numbers (AEN). Independent of the extant model, the estimated dinosaur AEN did not exceed 850 eggs (75,000 kg sauropod) for any of the taxa studied. This estimated maximum is probably an overestimation due to unrealistic assumptions. According to most AEN estimations, the dinosaurs studied laid less than 200 eggs per year. Only some AEN estimates obtained for medium to large sized sauropods were higher (200-400 eggs). Our results provide new (testable) hypotheses, especially for reproductive traits that are insufficiently

  4. Direct reticular projections of trigeminal sensory fibers immunoreactive to CGRP: potential monosynaptic somatoautonomic projections.

    PubMed

    Panneton, W Michael; Gan, Qi

    2014-01-01

    Few trigeminal sensory fibers project centrally beyond the trigeminal sensory complex, with only projections of fibers carried in its sensory anterior ethmoidal (AEN) and intraoral nerves described. Fibers of the AEN project into the brainstem reticular formation where immunoreactivity against substance P and CGRP are found. We investigated whether the source of these peptides could be from trigeminal ganglion neurons by performing unilateral rhizotomies of the trigeminal root and looking for absence of label. After an 8-14 days survival, substance P immunoreactivity in the trigeminal sensory complex was diminished, but we could not conclude that the sole source of this peptide in the lateral parabrachial area and lateral reticular formation arises from primary afferent fibers. Immunoreactivity to CGRP after rhizotomy however was greatly diminished in the trigeminal sensory complex, confirming the observations of others. Moreover, CGRP immunoreactivity was nearly eliminated in fibers in the lateral parabrachial area, the caudal ventrolateral medulla, both the peri-ambiguus and ventral parts of the rostral ventrolateral medulla, in the external formation of the nucleus ambiguus, and diminished in the caudal pressor area. The nearly complete elimination of CGRP in the lateral reticular formation after rhizotomy suggests this peptide is carried in primary afferent fibers. Moreover, the arborization of CGRP immunoreactive fibers in these areas mimics that of direct projections from the AEN. Since electrical stimulation of the AEN induces cardiorespiratory adjustments including an apnea, peripheral vasoconstriction, and bradycardia similar to those seen in the mammalian diving response, we suggest these perturbations of autonomic behavior are enhanced by direct somatic primary afferent projections to these reticular neurons. We believe this to be first description of potential direct somatoautonomic projections to brainstem neurons regulating autonomic activity.

  5. “Black Esophagus” or Gurvits Syndrome: A Rare Complication of Diabetic Ketoacidosis

    PubMed Central

    Dave, Kairavee; Cantave, Rulz; Shaharyar, Sameer; Joseph, Jeevan; Shankar, Uday; Kaplan, Steven; Feiz, Hamid

    2017-01-01

    Acute esophageal necrosis (AEN) also known as “black esophagus” or necrotizing esophagitis is a rare syndrome characterized by a striking diffuse patchy or circumferential black appearance of the esophageal mucosa that preferentially affects the distal esophagus and terminates at the gastroesophageal junction. Only 88 patients over a span of 40 years have received this diagnosis, and the prevalence of this disease ranges from 0.001 to 0.2% of cases in literature. It more commonly affects men (4 : 1 ratio) in the sixth decade of life. It is associated with a high mortality rate, approaching 32%. We report a case of AEN presenting in the setting of diabetic ketoacidosis (DKA), affecting both the proximal and distal esophagus. PMID:28357144

  6. Trigeminal Medullary Dorsal Horn Neurons Activated by Nasal Stimulation Coexpress AMPA, NMDA, and NK1 Receptors

    PubMed Central

    McCulloch, P. F.; DiNovo, K. M.; Westerhaus, D. J.; Vizinas, T. A.; Peevey, J. F.; Lach, M. A.; Czarnocki, P.

    2013-01-01

    Afferent information initiating the cardiorespiratory responses during nasal stimulation projects from the nasal passages to neurons within the trigeminal medullary dorsal horn (MDH) via the anterior ethmoidal nerve (AEN). Central AEN terminals are thought to release glutamate to activate the MDH neurons. This study was designed to determine which neurotransmitter receptors (AMPA, kainate, or NMDA glutamate receptor subtypes or the Substance P receptor NK1) are expressed by these activated MDH neurons. Fos was used as a neuronal marker of activated neurons, and immunohistochemistry combined with epifluorescent microscopy was used to determine which neurotransmitter receptor subunits were coexpressed by activated MDH neurons. Results indicate that, during nasal stimulation with ammonia vapors in urethane-anesthetized Sprague-Dawley rats, activated neurons within the superficial MDH coexpress the AMPA glutamate receptor subunits GluA1 (95.8%) and GluA2/3 (88.2%), the NMDA glutamate receptor subunits GluN1 (89.1%) and GluN2A (41.4%), and NK1 receptors (64.0%). It is therefore likely that during nasal stimulation the central terminals of the AEN release glutamate and substance P that then produces activation of these MDH neurons. The involvement of AMPA and NMDA receptors may mediate fast and slow neurotransmission, respectively, while NK1 receptor involvement may indicate activation of a nociceptive pathway. PMID:24967301

  7. Preverbal Particles in Pingelapese: A Language of Micronesia

    ERIC Educational Resources Information Center

    Hattori, Ryoko

    2012-01-01

    This dissertation presents a synchronic and diachronic study of Pingelapese pronouns and auxiliary verbs--"ae", "e", "aen", and "en". Synchronically, Pingelapese employs subject pronominal clitics, not subject agreement markers, unlike Proto-Micronesian and many other contemporary Micronesian languages.…

  8. Exact distinction of excitatory and inhibitory neurons in neural networks: a study with GFP-GAD67 neurons optically and electrophysiologically recognized on multielectrode arrays

    PubMed Central

    Becchetti, Andrea; Gullo, Francesca; Bruno, Giuseppe; Dossi, Elena; Lecchi, Marzia; Wanke, Enzo

    2012-01-01

    Distinguishing excitatory from inhibitory neurons with multielectrode array (MEA) recordings is a serious experimental challenge. The current methods, developed in vitro, mostly rely on spike waveform analysis. These however often display poor resolution and may produce errors caused by the variability of spike amplitudes and neuron shapes. Recent recordings in human brain suggest that the spike waveform features correlate with time-domain statistics such as spiking rate, autocorrelation, and coefficient of variation. However, no precise criteria are available to exactly assign identified units to specific neuronal types, either in vivo or in vitro. To solve this problem, we combined MEA recording with fluorescence imaging of neocortical cultures from mice expressing green fluorescent protein (GFP) in GABAergic cells. In this way, we could sort out “authentic excitatory neurons” (AENs) and “authentic inhibitory neurons” (AINs). We thus characterized 1275 units (from 405 electrodes, n = 10 experiments), based on autocorrelation, burst length, spike number (SN), spiking rate, squared coefficient of variation, and Fano factor (FF) (the ratio between spike-count variance and mean). These metrics differed by about one order of magnitude between AINs and AENs. In particular, the FF turned out to provide a firing code which exactly (no overlap) recognizes excitatory and inhibitory units. The difference in FF between all of the identified AEN and AIN groups was highly significant (p < 10−8, ANOVA post-hoc Tukey test). Our results indicate a statistical metric-based approach to distinguish excitatory from inhibitory neurons independently from the spike width. PMID:22973197

  9. An Unusual Cause of Acute Upper Gastrointestinal Bleeding: Acute Esophageal Necrosis

    PubMed Central

    Tokala, Madhusudhan R.; Dhillon, Sonu; Pisoh, Watcoun-Nchinda; Walayat, Saqib; Vanar, Vishwas; Puli, Srinivas R.

    2016-01-01

    Acute esophageal necrosis (AEN), also called “black esophagus,” is a condition characterized by circumferential necrosis of the esophagus with universal distal involvement and variable proximal extension with clear demarcation at the gastroesophageal junction. It is an unusual cause of upper gastrointestinal bleeding and is recognized with distinct and striking mucosal findings on endoscopy. The patients are usually older and are critically ill with shared comorbidities, which include atherosclerotic cardiovascular disease, diabetes mellitus, hypertension, chronic renal insufficiency, and malnutrition. Alcoholism and substance abuse could be seen in younger patients. Patients usually have systemic hypotension along with upper abdominal pain in the background of clinical presentation of hematemesis and melena. The endoscopic findings confirm the diagnosis and biopsy is not always necessary unless clinically indicated in atypical presentations. Herein we present two cases with distinct clinical presentation and discuss the endoscopic findings along with a review of the published literature on the management of AEN. PMID:27642529

  10. Black esophagus: Acute esophageal necrosis syndrome

    PubMed Central

    Gurvits, Grigoriy E

    2010-01-01

    Acute esophageal necrosis (AEN), commonly referred to as “black esophagus”, is a rare clinical entity arising from a combination of ischemic insult seen in hemodynamic compromise and low-flow states, corrosive injury from gastric contents in the setting of esophago-gastroparesis and gastric outlet obstruction, and decreased function of mucosal barrier systems and reparative mechanisms present in malnourished and debilitated physical states. AEN may arise in the setting of multiorgan dysfunction, hypoperfusion, vasculopathy, sepsis, diabetic ketoacidosis, alcohol intoxication, gastric volvulus, traumatic transection of the thoracic aorta, thromboembolic phenomena, and malignancy. Clinical presentation is remarkable for upper gastrointestinal bleeding. Notable symptoms may include epigastric/abdominal pain, vomiting, dysphagia, fever, nausea, and syncope. Associated laboratory findings may reflect anemia and leukocytosis. The hallmark of this syndrome is the development of diffuse circumferential black mucosal discoloration in the distal esophagus that may extend proximally to involve variable length of the organ. Classic “black esophagus” abruptly stops at the gastroesophageal junction. Biopsy is recommended but not required for the diagnosis. Histologically, necrotic debris, absence of viable squamous epithelium, and necrosis of esophageal mucosa, with possible involvement of submucosa and muscularis propria, are present. Classification of the disease spectrum is best described by a staging system. Treatment is directed at correcting coexisting clinical conditions, restoring hemodynamic stability, nil-per-os restriction, supportive red blood cell transfusion, and intravenous acid suppression with proton pump inhibitors. Complications include perforation with mediastinal infection/abscess, esophageal stricture and stenosis, superinfection, and death. A high mortality of 32% seen in the setting of AEN syndrome is usually related to the underlying medical co

  11. Acute esophageal necrosis and liver pathology, a rare combination

    PubMed Central

    Khan, Amir Maqbul; Hundal, Rangit; Ramaswamy, Vijaya; Korsten, Mark; Dhuper, Sunil

    2004-01-01

    Acute esophageal necrosis (AEN) or “black esophagus” is a clinical condition found at endoscopy. It is a rare entity the exact etiology of which remains unknown. We describe a case of ‘black esophagus’, first of its kind, in the setting of liver cirrhosis and hepatic encephalopathy. PMID:15285044

  12. Acute Esophageal Necrosis: A Case Series

    PubMed Central

    Mavilia, Marianna G; Gurvits, Grigoriy E

    2018-01-01

    Acute esophageal necrosis (AEN) is a particularly rare syndrome with an incidence of only 0.1-0.28%, whose appearance is notable for proximal extensions of black, necrotic appearing mucosa extending proximally in the esophagus and abruptly interrupted at the gastroesophageal junction. In this case series, we explore the cases of two males: one middle-aged and one elderly, who after presenting with emesis, were found to have acute esophageal necrosis on esophagogastroduodenoscopy. PMID:29850386

  13. Environmental Impact Statement. Space Nuclear Thermal Propulsion Program. Particle Bed reactor Propulsion Technology Development and Validation

    DTIC Science & Technology

    1993-05-01

    further examination or disposal. 2.2.2.3 Non -Nucleer Engine Integration Tests. EITs would be designed to demonstrate proper function of the propellant...located 42 miles southwest of the CTF, is designated as a Class I air quality region. The nearest non -attainment area is Pocatello, Idaho, 75 miles south of...accelerate. combustiomn. Nintrogen and helnee are Staple CEnergy suggestsa design eail ofAt a em-le 500 aenIIirons 1 aoph,,,,ants ad non -reastive. ?annual

  14. Impact of Acoustic Loads on Aircraft Structures (Impact des Solicitations Acoustiques sur les Structures d’Aeronefs)

    DTIC Science & Technology

    1994-09-01

    of te1 e B Iti-me reater thany engine aen both operating. Sound levels on the This nestle beat flux is too times eater than Jlower half of the...torso simulator was used in nlight for the binaural recording of noise and conmmunication Moreover the use of pasive tnot*: control mteasures... beating ", of the propellers. It is then recog.point due to a source at the first [10, 11]. Hence, rather nized that the sound fields of the individual

  15. Technical Papers Presented at the Defense Nuclear Agency Global Effects Review. Held at Moffett Field, California on 25-27 February 1986. Volume 3.

    DTIC Science & Technology

    1986-05-15

    rvf I 4 .4>* .~1 .4% L...8217- 6 M 0000 0- t- CIO Y) I- n006 0 0 ~ 73 2 G 0 0 NN na) -z C ta dOC "? 0 0 R Uc IlD li _D_ CDI CDI (se)ao w e p- lq aen no eForage-cow- milk , a Pasture...Concentration in milk , a Dose per unit activity ingested, a Infant’s milk consumption rate. * 6(a) little or no effect, W(b slight to moderate effect,

  16. Sony’s Nightmare before Christmas: The 2014 North Korean Cyber Attack on Sony and Lessons for US Government Actions in Cyberspace

    DTIC Science & Technology

    2018-02-01

    4,  2014), http://www.businessinsider.com/ experts-the-sony-hack-looks-a-lot-like-previous-attacks-on- south-korea-2014-12. 39 Kim Zetter, “Logic Bomb ...Set Off South Korea Cyberattack,” Wired (March 21, 2013), https://www.wired.com/2013/03/logic- bomb -south-korea-attack/. 40 Brian Krebs, “The Case...Agency (March  21,  2013), http://english.yonhapnews.co.kr/ national/2013/03/21/55/0302000000AEN20130321003552315F. HTML. 47 Kim Zetter, “Logic Bomb

  17. Mass energy-absorption coefficients and average atomic energy-absorption cross-sections for amino acids in the energy range 0.122-1.330 MeV

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    More, Chaitali V., E-mail: chaitalimore89@gmail.com; Lokhande, Rajkumar M.; Pawar, Pravina P., E-mail: pravinapawar4@gmail.com

    Mass attenuation coefficients of amino acids such as n-acetyl-l-tryptophan, n-acetyl-l-tyrosine and d-tryptophan were measured in the energy range 0.122-1.330 MeV. NaI (Tl) scintillation detection system was used to detect gamma rays with a resolution of 8.2% at 0.662 MeV. The measured attenuation coefficient values were then used to determine the mass energy-absorption coefficients (σ{sub a,en}) and average atomic energy-absorption cross sections (μ{sub en}/ρ) of the amino acids. Theoretical values were calculated based on XCOM data. Theoretical and experimental values are found to be in good agreement.

  18. A Medical Monitoring Program for the Marine Hazardous Chemical Worker. Volume 1

    DTIC Science & Technology

    1985-12-01

    ri-CR ESOL CRL 0 O-CRESOL CSL 0 P-CRESOL CSO 0 C RE SOL S CRS 0 CRESYLATE SPENT CAUSTIC CSC 0 CROTONALDEH’YDE CTA -0 C UPINE cuml 0 CYCLOHEXANE CHX 0...BLACk BASE 0 CARBON DISULIFIDE CBS 0 CARBON MONOG IDE CARBON TETRACHLORIDE CST 0 CAUSTIC POTASH SOLUTION CPS a CAUSTIC SODA SOLUTION CSS 0 CETYL ALCOHOL...HXN D 1 -𔃾.AENE HXE Ci HEXYLENE *;i -COL HX(G 0 H, TrClCHL0 . ACID 14CL 13 H’vDPC-wLO’.. ACID. SPENT (15%. OP LESS) Hcs C) HYSý90F! tOJCQ AC 10 HFA 0 H

  19. Operational aspects of asynchronous filtering for improved flood forecasting

    NASA Astrophysics Data System (ADS)

    Rakovec, Oldrich; Weerts, Albrecht; Sumihar, Julius; Uijlenhoet, Remko

    2014-05-01

    Hydrological forecasts can be made more reliable and less uncertain by recursively improving initial conditions. A common way of improving the initial conditions is to make use of data assimilation (DA), a feedback mechanism or update methodology which merges model estimates with available real world observations. The traditional implementation of the Ensemble Kalman Filter (EnKF; e.g. Evensen, 2009) is synchronous, commonly named a three dimensional (3-D) assimilation, which means that all assimilated observations correspond to the time of update. Asynchronous DA, also called four dimensional (4-D) assimilation, refers to an updating methodology, in which observations being assimilated into the model originate from times different to the time of update (Evensen, 2009; Sakov 2010). This study investigates how the capabilities of the DA procedure can be improved by applying alternative Kalman-type methods, e.g., the Asynchronous Ensemble Kalman Filter (AEnKF). The AEnKF assimilates observations with smaller computational costs than the original EnKF, which is beneficial for operational purposes. The results of discharge assimilation into a grid-based hydrological model for the Upper Ourthe catchment in Belgian Ardennes show that including past predictions and observations in the AEnKF improves the model forecasts as compared to the traditional EnKF. Additionally we show that elimination of the strongly non-linear relation between the soil moisture storage and assimilated discharge observations from the model update becomes beneficial for an improved operational forecasting, which is evaluated using several validation measures. In the current study we employed the HBV-96 model built within a recently developed open source modelling environment OpenStreams (2013). The advantage of using OpenStreams (2013) is that it enables direct communication with OpenDA (2013), an open source data assimilation toolbox. OpenDA provides a number of algorithms for model calibration

  20. Finite Element Program for Calculating Flows in Turbomachines with Results for NASA Task-1 Compressor.

    DTIC Science & Technology

    1982-10-01

    CR !PT Jr,4 CF THE FUPEP ELiAEN5 + f ,, 4, S**444444**44*4*4*444++ 44 4.+414++++++4++ + +++4+++44 f 44*4* ++ +444. + 172 FILE: IdES!’ GEN FC4TRAN Al...CLCC-(-"II e(1) 21...X’) STE’T ST’ICT *SSICT 179 FILE: PESI- GEN FCRTPAN Al NAVNL POSTGRAOLATE SCHO󈧣 70C CCITTNJUE P~lUPN C *****+*+*+4*+ *.4+..*4.4...PEtC IN fCCiNFC7Iv1TY IPATP:X, Lr..%L NdCDE NLVhJ3E S STAPT AT ELVEPTIS i.;PIi iR!G~e r~ fANC LC,.% IND) TAV -QSi Mih. C REIC IN 11-E FLEPOENT TypE

  1. Acute Esophageal Necrosis: “Black Esophagus”

    PubMed Central

    Weigel, Tracey L.

    2007-01-01

    Acute esophageal necrosis (AEN) is an uncommon event. We report a case of an 84-year-old female with a giant paraesophageal hernia who presented with coffee ground emesis and on esophagogastroduodenoscopy (EGD) demonstrated findings consistent with acute esophageal necrosis and a giant paraesophageal hernia with normal-appearing gastric mucosa. She was managed conservatively with bowel rest, parenteral nutrition, and continuous intravenous proton pump inhibitor (PPI). After significant improvement in the gross appearance of her esophageal mucosa, surgery was performed to reduce her giant paraesophageal hernia. The patient's postoperative course was uneventful, and she was discharged home on postoperative day 6, tolerating a normal diet. The percutaneous endoscopic gastrostomy (PEG) tube was removed in clinic 2 months postoperatively. PMID:17651583

  2. Participatory data collection and monitoring of agricultural pest dynamics for climate-resilient coffee production using Tiko'n, a generic tool to develop agroecological food web models

    NASA Astrophysics Data System (ADS)

    Rojas, M.; Malard, J. J.; Adamowski, J. F.; Tuy, H.

    2016-12-01

    Climate variability impacts agricultural processes through many mechanisms. For example, the proliferation of pests and diseases increases with warmer climate and alternated wind patterns, as longer growing seasons allow pest species to complete more reproductive cycles and changes in the weather patterns alter the stages and rates of development of pests and pathogens. Several studies suggest that enhancing plant diversity and complexity in farming systems, such as in agroforestry systems, reduces the vulnerability of farms to extreme climatic events. On the other hand, other authors have argued that vegetation diversity does not necessarily reduce the incidence of pests and diseases, highlighting the importance of understanding how, where and when it is recommendable to diversify vegetation to improve pest and disease control, and emphasising the need for tools to develop, monitor and evaluate agroecosystems. In order to understand how biodiversity can enhance ecosystem services provided by the agroecosystem in the context of climatic variability, it is important to develop comprehensive models that include the role of trophic chains in the regulation of pests, which can be achieved by integrating crop models with pest-predator models, also known as agroecosystem network (AEN) models. Here we present a methodology for the participatory data collection and monitoring necessary for running Tiko'n, an AEN model that can also be coupled to a crop model such as DSSAT. This methodology aims to combine the local and practical knowledge of farmers with the scientific knowledge of entomologists and agronomists, allowing for the simplification of complex ecological networks of plant and insect interactions. This also increases the acceptability, credibility, and comprehension of the model by farmers, allowing them to understand their relationship with the local agroecosystem and their potential to use key agroecosystem principles such as functional diversity to mitigate

  3. Manipulating Planting Density and Nitrogen Fertilizer Application to Improve Yield and Reduce Environmental Impact in Chinese Maize Production

    PubMed Central

    Xu, Cailong; Huang, Shoubing; Tian, Beijing; Ren, Jianhong; Meng, Qingfeng; Wang, Pu

    2017-01-01

    Relatively low nitrogen (N) efficiency and heavy environmental costs caused by excessive N fertilizer applications with outdated fertilization techniques are current cultivation production problems with maize among smallholders in North China Plain. Although many studies have examined agronomical strategies for improving yields and N use, the integrated effects of these measures and the associated environmental costs are not well understood. We conducted a 2-year field study with two densities (67,500 plants ha-1, which was similar to local farmers’ practices, and 90,000 plants ha-1) and three N rates (0, 180, and 360 kg ha-1, the rate local farmers’ commonly apply) to test the integrated effects for maize production at Wuqiao experimental station in North China Plain. The higher planting density produced significant increases in grain yield (GY), N use efficiency (NUE), agronomic N efficiency (AEN), and N partial productivity (PFPN) by 6.6, 3.9, 24.7, and 8.8%, respectively; in addition, N2O emission and greenhouse gas intensity decreased by 7.3 and 4.3%, respectively. With a lower N application rate, from 360 to 180 kg ha-1, GY was unchanged, and NUE, AEN, and PFPN all significantly increased by 6.2, 96.0, and 98.7%, respectively; in addition, N2O emission and greenhouse gas intensity decreased by 61.5 and 46.2%, respectively. The optimized N rate (180 kg N ha-1) for the 90,000 plants ha-1 treatment achieved the highest yield with only 50% of the N fertilizer input commonly employed by local farmers’ (360 kg N ha-1), which contributed to the increased N-uptake and N-transfer capacity. Therefore, our study demonstrated that agronomical methods such as increasing planting density with reasonable N application could be useful to obtain higher GY along with efficient N management to help lower environmental costs of maize production. PMID:28747925

  4. Forsterite-Anorthite-CaTschermak-Enstatite (FACE): A geobarometer for plagioclase-bearing peridotites

    NASA Astrophysics Data System (ADS)

    Fumagalli, P.; Borghini, G.; Rampone, E.; Poli, S.

    2017-12-01

    Plagioclase peridotites can be the result of either metamorphic recrystallization, or diffuse melt-rock interactions. Although they represent an important geodynamic marker of shallow mantle exhumation, the accurate estimation of their barometric evolution is poorly constrained. Systematic correlations between pressure and composition of coexisting minerals within the plagioclase stability field have been reported in previous experimental studies. In this experimental study we present new constraints on the stability of plagioclase as a function of different Na2O/CaO bulk ratios, and we present a new geobarometer for mantle rocks. Experiments have been performed in a single-stage piston cylinder at 5-10 kbar, 1050-1150 °C at nominally anhydrous conditions using seeded gels of peridotite compositions (Na2O/CaO = 0.08-0.13; XCr = Cr/(Cr + Al) = 0.07-0.10). As expected, the increase of the bulk Na2O/CaO extends the plagioclase stability to higher pressure; in the Na2O enriched fertile lherzolite the plagioclase-spinel transition occurs between 9 and 10 kbar, 1100 °C; in the fertile lherzolite with Na2O/CaO = 0.08, it occurs between 8 and 9 kbar, 1100 °C. The present data together with previous experimental results provide a consistent database, covering a wide range of P-T conditions (3-9 kbar, 1000-1150 °C) and variable bulk compositions to be used to define and calibrate a geobarometer for plagioclase-bearing mantle rocks. We have empirically calibrated by least squares regression analysis of experimental data combined with MonteCarlo simulation the following pressure sensitive equilibrium: Mg2SiO4 Ol + CaAl2Si2O8 Pl = CaAl2SiO6 Cpx + Mg2Si2O6 OpxForsterite Anorthite Ca-Tschermak Enstatite We derive the following equation: P = 7.2 (±2.9) + 0.0078 (±0.0021) T + 0.0022 (±0.0001) T lnK R2= 0.93 where P is expressed in kbar and T in kelvin. K is the equilibrium constant K = aCa-Ts*aen / aan *afo, where aCaTs, aen, aan and afo are the activities of Ca-Tschermak in

  5. Agronomic Characteristics Related to Grain Yield and Nutrient Use Efficiency for Wheat Production in China

    PubMed Central

    Zheng, Huaiguo; Xu, Xinpeng

    2016-01-01

    In order to make clear the recent status and trend of wheat (Triticum aestivum L.) production in China, datasets from multiple field experiments and published literature were collected to study the agronomic characteristics related to grain yield, fertilizer application and nutrient use efficiency from the year 2000 to 2011. The results showed that the mean grain yield of wheat in 2000–2011 was 5950 kg/ha, while the N, P2O5 and K2O application rates were 172, 102 and 91 kg/ha on average, respectively. The decrease in N and P2O5 and increase in K2O balanced the nutrient supply and was the main reason for yield increase. The partial factor productivity (PFP, kg grain yield produced per unit of N, P2O5 or K2O applied) values of N (PFP-N), P (PFP-P) and K (PFP-K) were in the ranges of 29.5~39.6, 43.4~74.9 and 44.1~76.5 kg/kg, respectively. While PFP-N showed no significant changes from 2000 to 2010, both PFP-P and PFP-K showed an increased trend over this period. The mean agronomic efficiency (AE, kg grain yield increased per unit of N, P2O5 or K2O applied) values of N (AEN), P (AEP) and K (AEK) were 9.4, 10.2 and 6.5 kg/kg, respectively. The AE values demonstrated marked inter-annual fluctuations, with the amplitude of fluctuation for AEN greater than those for AEP and AEK. The mean fertilizer recovery efficiency (RE, the fraction of nutrient uptake in aboveground plant dry matter to the nutrient of fertilizer application) values of N, P and K in the aboveground biomass were 33.1%, 24.3% and 28.4%, respectively. It was also revealed that different wheat ecological regions differ greatly in wheat productivity, fertilizer application and nutrient use efficiency. In summary, it was suggested that best nutrient management practices, i.e. fertilizer recommendation applied based on soil testing or yield response, with strategies to match the nutrient input with realistic yield and demand, or provided with the 4R’s nutrient management (right time, right rate, right site

  6. Nitrogen use efficiency in grain production and the estimated nitrogen input/output balance in China agriculture.

    PubMed

    Li, Shutian; He, Ping; Jin, Jiyun

    2013-03-30

    Understanding the nitrogen (N) use efficiency and N input/output balance in the agricultural system is crucial for best management of N fertilisers in China. In the last 60 years, N fertiliser consumption correlated positively with grain production. During that period the partial factor productivity of N (PFPN ) declined greatly from more than 1000 kg grain kg⁻¹ N in the 1950s to nearly 30 kg grain kg⁻¹ N in 2008. This change in PFPN could be largely explained by the increase in N rate. The average agronomic efficiency of fertiliser N (AEN ) for rice, wheat and maize during 2000-2010 was 12.6, 8.3 and 11.5 kg kg⁻¹ respectively, which was similar to that in the early 1980s but lower than that in the early 1960s. Estimation based on statistical data showed that a total of 49.16 × 10⁶ t of N was input into Chinese agriculture, of which chemical N, organic fertiliser N, biological fixed N and other sources accounted for 58.2, 24.3, 10.5 and 7.0% respectively. Nitrogen was surplus in all regions, the total N surplus being 10.6 × 10⁶ t (60.6 kg ha⁻¹). The great challenge is to balance the use of current N fertilisers between regions and crops to improve N use efficiency while maintaining or increasing crop production under the high-intensity agricultural system of China. © 2012 Society of Chemical Industry.

  7. Choosing the target of adaptive soil erosion management in Mediterranean. Long vs. Extreme erosion, internal vs. external catchment dynamics

    NASA Astrophysics Data System (ADS)

    Smetanova, Anna; Follain, Stéphane; David, Mélodie; Ciampalini, Rossano; Raclot, Damien; Crabit, Armand; Le Bissonnais, Yves

    2017-04-01

    For soil resources protection and regulation of soil erosion off-site effects in Mediterranean, it is inevitable to adjust current land management planning to both, event magnitude and long-term erosion means [2, 3, 5]. Science-based soil protection measures need to be adjusted to spatial and temporal scale of practice differing between stakeholders and management aims, and reflect increasing frequency of torrential rainfalls leading to very high erosion rates in short time [3, 4]. In order to address selection of zero-soil erosion land management target, this study applies modelling approach for comparison of 7 land use scenarios using the LandSoil model [1]. We propose comparison of internal vs. external catchment dynamic at extreme event- and long-term scale as a tool for understanding effect of land management in targeting emerging erosion and connectivity patterns. Our results suggest, that proposed approach can be applied to identify best management scenario practices regarding different management aims of farmers and watershed managers. [1] Ciampalini R, Follain S, Le Bissonnais Y. 2012. LandSoil: A model for analysing the impact of erosion on agricultural landscape evolution. Geomorphology 175-176: 25-37. [2] David M, Follain S, Ciampalini R, Le Bissonnais Y, Couturier A, Walter C. 2014. Simulation of medium-term soil redistributions for different land use and landscape design scenarios within a vineyard landscape in Mediterranean France. Geomorphology 214: 10-21. [3] Smetanová A, Le Bissonnais Y, Raclot D, Nunes JP, Licciardello F, Le Bouteiller C, Latron J, Rodríguez-Caballero E, Mathys N, Klotz S, Mekki I, Gallart F, Solé Benet A, Pérez Gallego N, Andrieux P, Moussa R, Planchon O, Marisa Santos J, Alshihabi O, Chikhaoui M., submitted. Patterns of temporal variability and time compression of sediment yield in small Mediterranean catchments. Soil Use & Management [4] Smetanová A, Paton E, Maynard C, Tindale S, Fernandez-Getino A-P, Marques MJ, Bracken

  8. UCHL1 Is a Putative Tumor Suppressor in Ovarian Cancer Cells and Contributes to Cisplatin Resistance

    PubMed Central

    Jin, Chengmeng; Yu, Wei; Lou, Xiaoyan; Zhou, Fan; Han, Xu; Zhao, Na; Lin, Biaoyang

    2013-01-01

    Ubiquitin carboxyl terminal hydrolase 1 (UCHL1) catalyzes the hydrolysis of COOH-terminal ubiquityl esters and amides. It has been reported as either an oncogene or a tumor suppressor in cancers. However, UCHL1's role in ovarian cancer is still unclear. Therefore, we conducted an analysis to understand the role of UCHL1 in ovarian cancer. Firstly, we detected UCHL1 promoter methylation status in 7 ovarian cancer cell lines. 4 of them with UCHL1 silencing showed heavy promoter methylation while the other 3 with relative high UCHL1 expression showed little promoter methylation. Then we reduced UCHL1 expression in ovarian cancer cell line A2780 and IGROV1 and found that inhibition of UCHL1 promoted cell proliferation by increasing cells in S phases of cell cycle. Knockdown of UCHL1 also reduced cell apoptosis and contributed to cisplatin resistance. Furthermore, the expression level of UCHL1 in several ovarian cancer cell lines correlated negatively with their cisplatin resistance levels. Microarray data revealed that UCHL1 related genes are enriched in apoptosis and cell death gene ontology (GO) terms. Several apoptosis related genes were increased after UCHL1 knockdown, including apoptosis regulator BCL2, BCL11A, AEN and XIAP. Furthermore, we identified up-regulation of Bcl-2 and pAKT as well as down-regulation of Bax in UCHL1 knockdown cells, while no significant alteration of p53 and AKT1 was found. This study provides a new and promising strategy to overcome cisplatin resistance in ovarian cancer via UCHL1 mediated pathways. PMID:24155778

  9. Suppression of Radiation-Induced Testicular Germ Cell Apoptosis by 2,5-Hexanedione Pretreatment. III. Candidate Gene Analysis Identifies a Role for Fas in the Attenuation of X-ray–Induced Apoptosis

    PubMed Central

    Campion, Sarah N.; Sandrof, Moses A.; Yamasaki, Hideki; Boekelheide, Kim

    2010-01-01

    Germ cell apoptosis directly induced by x-radiation (x-ray) exposure is stage specific, with a higher incidence in stage II/III seminiferous tubules. A priming exposure to the Sertoli cell toxicant 2,5-hexanedione (HD) results in a marked reduction in x-ray–induced germ cell apoptosis in these affected stages. Because of the stage specificity of these responses, examination of associated gene expression in whole testis tissue has clear limitations. Laser capture microdissection (LCM) of specific cell populations in the testis is a valuable technique for investigating the responses of different cell types following toxicant exposure. LCM coupled with quantitative real-time PCR was performed to examine the expression of apoptosis-related genes at both early (3 h) and later (12 h) time points after x-ray exposure, with or without the priming exposure to HD. The mRNAs examined include Fas, FasL, caspase 3, bcl-2, p53, PUMA, and AEN, which were identified either by literature searches or microarray analysis. Group 1 seminiferous tubules (stages I–VI) exhibited the greatest changes in gene expression. Further analysis of this stage group (SG) revealed that Fas induction by x-ray is significantly attenuated by HD co-exposure. Selecting only for germ cells from seminiferous tubules of the most sensitive SG has provided further insight into the mechanisms involved in the co-exposure response. It is hypothesized that following co-exposure, germ cells adapt to the lack of Sertoli cell support by reducing the Fas response to normal FasL signals. These findings provide a better understanding and appreciation of the tissue complexity and technical difficulties associated with examining gene expression in the testis. PMID:20616204

  10. A high plant density reduces the ability of maize to use soil nitrogen

    PubMed Central

    Yan, Peng; Pan, Junxiao; Zhang, Wenjie; Shi, Junfang; Chen, Xinping; Cui, Zhenling

    2017-01-01

    Understanding the physiological changes associated with high grain yield and high N use efficiency (NUE) is important when increasing the plant density and N rate to develop optimal agronomic management. We tested the hypothesis that high plant densities resulting in crowding stress reduce the ability of plants to use the N supply post-silking, thus decreasing the grain yield and NUE. In 2013 and 2014, a field experiment, with five N-application rates and three plant densities (6.0, 7.5, and 9.0 plants m–2), was conducted in the North China Plain (NCP). The calculated maximum grain yield and agronomic use efficiency (AEN) at a density of 7.5 plants m–2 were 12.4 Mg ha–1 and 39.3 kg kg–1, respectively, which were significantly higher than the values obtained at densities of 6.0 (11.3 Mg ha–1 and 30.2 kg kg–1) and 9.0 plant m–2 (11.7 Mg ha–1 and 27.8 kg kg–1). A high plant density of 9.0 plants m–2 decreased the post-silking N accumulation, leaf N concentration and net photosynthesis, which reduced the post-silking dry matter production, resulting in a low yield and NUE. Although a relatively low grain yield was observed at a density of 9.0 plants m–2, the optimal N rate increased from 150 to 186 kg N ha-1 at a density of 7.5 plants m–2. These results indicate that high plant densities with crowding stress reduce the ability of plants to use soil N during the post-silking period, and high rate of N fertilizer was needed to increase grain yield. We conclude that selecting the appropriate plant density combined with optimal N management could increase grain yields and the NUE in the NCP. PMID:28234970

  11. Impact of Neutron Exposure on Global Gene Expression in a Human Peripheral Blood Model

    PubMed Central

    Broustas, Constantinos G.; Xu, Yanping; Harken, Andrew D.; Chowdhury, Mashkura; Garty, Guy; Amundson, Sally A.

    2017-01-01

    The detonation of an improvised nuclear device would produce prompt radiation consisting of both photons (gamma rays) and neutrons. While much effort in recent years has gone into the development of radiation biodosimetry methods suitable for mass triage, the possible effect of neutrons on the endpoints studied has remained largely uninvestigated. We have used a novel neutron irradiator with an energy spectrum based on that 1–1.5 km from the epicenter of the Hiroshima blast to begin examining the effect of neutrons on global gene expression, and the impact this may have on the development of gene expression signatures for radiation biodosimetry. We have exposed peripheral blood from healthy human donors to 0.1, 0.3, 0.5 or 1 Gy of neutrons ex vivo using our neutron irradiator, and compared the transcriptomic response 24 h later to that resulting from sham exposure or exposure to 0.1, 0.3, 0.5, 1, 2 or 4 Gy of photons (X rays). We identified 125 genes that responded significantly to both radiation qualities as a function of dose, with the magnitude of response to neutrons generally being greater than that seen after X-ray exposure. Gene ontology analysis suggested broad involvement of the p53 signaling pathway and general DNA damage response functions across all doses of both radiation qualities. Regulation of immune response and chromatin-related functions were implicated only following the highest doses of neutrons, suggesting a physiological impact of greater DNA damage. We also identified several genes that seem to respond primarily as a function of dose, with less effect of radiation quality. We confirmed this pattern of response by quantitative real-time RT-PCR for BAX, TNFRSF10B, ITLN2 and AEN and suggest that gene expression may provide a means to differentiate between total dose and a neutron component. PMID:28140791

  12. Impact of Neutron Exposure on Global Gene Expression in a Human Peripheral Blood Model.

    PubMed

    Broustas, Constantinos G; Xu, Yanping; Harken, Andrew D; Chowdhury, Mashkura; Garty, Guy; Amundson, Sally A

    2017-04-01

    The detonation of an improvised nuclear device would produce prompt radiation consisting of both photons (gamma rays) and neutrons. While much effort in recent years has gone into the development of radiation biodosimetry methods suitable for mass triage, the possible effect of neutrons on the endpoints studied has remained largely uninvestigated. We have used a novel neutron irradiator with an energy spectrum based on that 1-1.5 km from the epicenter of the Hiroshima blast to begin examining the effect of neutrons on global gene expression, and the impact this may have on the development of gene expression signatures for radiation biodosimetry. We have exposed peripheral blood from healthy human donors to 0.1, 0.3, 0.5 or 1 Gy of neutrons ex vivo using our neutron irradiator, and compared the transcriptomic response 24 h later to that resulting from sham exposure or exposure to 0.1, 0.3, 0.5, 1, 2 or 4 Gy of photons (X rays). We identified 125 genes that responded significantly to both radiation qualities as a function of dose, with the magnitude of response to neutrons generally being greater than that seen after X-ray exposure. Gene ontology analysis suggested broad involvement of the p53 signaling pathway and general DNA damage response functions across all doses of both radiation qualities. Regulation of immune response and chromatin-related functions were implicated only following the highest doses of neutrons, suggesting a physiological impact of greater DNA damage. We also identified several genes that seem to respond primarily as a function of dose, with less effect of radiation quality. We confirmed this pattern of response by quantitative real-time RT-PCR for BAX, TNFRSF10B, ITLN2 and AEN and suggest that gene expression may provide a means to differentiate between total dose and a neutron component.

  13. Identification of Differential Gene Expression Patterns after Acute Exposure to High and Low Doses of Low-LET Ionizing Radiation in a Reconstituted Human Skin Tissue

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Tilton, Susan C.; Markillie, Lye Meng; Hays, Spencer

    Our goal here was to identify dose and temporal dependent radiation responses in a complex tissue, reconstituted human skin. Direct sequencing of RNA (RNA-seq) was used to quantify altered transcripts following exposure to 0.1, 2 and 10 Gy of ionizing radiation at 3 and 8 hours. These doses include a low dose in the range of some medical diagnostic procedures (0.1 Gy), a dose typically received during radiotherapy (2.0 Gy) and a lethal dose (10 Gy). These doses could be received after an intentional or accidental radiation exposure and biomarkers are needed to rapidly and accurately triage exposed individuals. Amore » total of 1701 genes were deemed to be significantly affected by high dose radiation exposure with the majority of genes affected at 10 Gy. A group of 29 genes including GDF15, BBC3, PPM1D, FDXR, GADD45A, MDM2, CDKN1A, TP53INP1, CYCSP27, SESN1, SESN2, PCNA, and AEN were similarly altered at both 2 and 10 Gy, but not 0.1 Gy, at multiple time points. A much larger group of up regulated genes, including those involved in inflammatory responses, was significantly altered only after a 10 Gy exposure. At high doses, down regulated genes were associated with cell cycle regulation and exhibited an apparent linear response between 2 and 10 Gy. While only a handful of genes were significantly affected by 0.1 Gy exposure using stringent statistical filters, groups of related genes regulating cell cycle progression and inflammatory responses consistently exhibited opposite trends in their regulation compared to the high dose exposures. Differential regulation of PLK1 signaling at low and high doses was confirmed using qRT-PCR. These results indicate that some alterations in gene expression are qualitatively different at low and high doses of radiation in this model system.« less

  14. Towards guided data assimilation for operational hydrologic forecasting in the US Tennessee River basin

    NASA Astrophysics Data System (ADS)

    Weerts, A.; Wood, A. W.; Clark, M. P.; Carney, S.; Day, G. N.; Lemans, M.; Sumihar, J.; Newman, A. J.

    2014-12-01

    In the US, the forecasting approach used by the NWS River Forecast Centers and other regional organizations such as the Bonneville Power Administration (BPA) or Tennessee Valley Authority (TVA) has traditionally involved manual model input and state modifications made by forecasters in real-time. This process is time consuming and requires expert knowledge and experience. The benefits of automated data assimilation (DA) as a strategy for avoiding manual modification approaches have been demonstrated in research studies (eg. Seo et al., 2009). This study explores the usage of various ensemble DA algorithms within the operational platform used by TVA. The final goal is to identify a DA algorithm that will guide the manual modification process used by TVA forecasters and realize considerable time gains (without loss of quality or even enhance the quality) within the forecast process. We evaluate the usability of various popular algorithms for DA that have been applied on a limited basis for operational hydrology. To this end, Delft-FEWS was wrapped (via piwebservice) in OpenDA to enable execution of FEWS workflows (and the chained models within these workflows, including SACSMA, UNITHG and LAGK) in a DA framework. Within OpenDA, several filter methods are available. We considered 4 algorithms: particle filter (RRF), Ensemble Kalman Filter and Asynchronous Ensemble Kalman and Particle filter. Retrospective simulation results for one location and algorithm (AEnKF) are illustrated in Figure 1. The initial results are promising. We will present verification results for these methods (and possible more) for a variety of sub basins in the Tennessee River basin. Finally, we will offer recommendations for guided DA based on our results. References Seo, D.-J., L. Cajina, R. Corby and T. Howieson, 2009: Automatic State Updating for Operational Streamflow Forecasting via Variational Data Assimilation, 367, Journal of Hydrology, 255-275. Figure 1. Retrospectively simulated

  15. Operational Hydrological Forecasting During the Iphex-iop Campaign - Meet the Challenge

    NASA Technical Reports Server (NTRS)

    Tao, Jing; Wu, Di; Gourley, Jonathan; Zhang, Sara Q.; Crow, Wade; Peters-Lidard, Christa D.; Barros, Ana P.

    2016-01-01

    An operational streamflow forecasting testbed was implemented during the Intense Observing Period (IOP) of the Integrated Precipitation and Hydrology Experiment (IPHEx-IOP) in May-June 2014 to characterize flood predictability in complex terrain. Specifically, hydrological forecasts were issued daily for 12 headwater catchments in the Southern Appalachians using the Duke Coupled surface-groundwater Hydrology Model (DCHM) forced by hourly atmospheric fields and QPFs (Quantitative Precipitation Forecasts) produced by the NASA-Unified Weather Research and Forecasting (NU-WRF) model. Previous day hindcasts forced by radar-based QPEs (Quantitative Precipitation Estimates) were used to provide initial conditions for present day forecasts. This manuscript first describes the operational testbed framework and workflow during the IPHEx-IOP including a synthesis of results. Second, various data assimilation approaches are explored a posteriori (post-IOP) to improve operational (flash) flood forecasting. Although all flood events during the IOP were predicted by the IPHEx operational testbed with lead times of up to 6 h, significant errors of over- and, or under-prediction were identified that could be traced back to the QPFs and subgrid-scale variability of radar QPEs. To improve operational flood prediction, three data-merging strategies were pursued post-IOP: (1) the spatial patterns of QPFs were improved through assimilation of satellite-based microwave radiances into NU-WRF; (2) QPEs were improved by merging raingauge observations with ground-based radar observations using bias-correction methods to produce streamflow hindcasts and associated uncertainty envelope capturing the streamflow observations, and (3) river discharge observations were assimilated into the DCHM to improve streamflow forecasts using the Ensemble Kalman Filter (EnKF), the fixed-lag Ensemble Kalman Smoother (EnKS), and the Asynchronous EnKF (i.e. AEnKF) methods. Both flood hindcasts and forecasts

  16. Operational hydrological forecasting during the IPHEx-IOP campaign - Meet the challenge

    NASA Astrophysics Data System (ADS)

    Tao, Jing; Wu, Di; Gourley, Jonathan; Zhang, Sara Q.; Crow, Wade; Peters-Lidard, Christa; Barros, Ana P.

    2016-10-01

    An operational streamflow forecasting testbed was implemented during the Intense Observing Period (IOP) of the Integrated Precipitation and Hydrology Experiment (IPHEx-IOP) in May-June 2014 to characterize flood predictability in complex terrain. Specifically, hydrological forecasts were issued daily for 12 headwater catchments in the Southern Appalachians using the Duke Coupled surface-groundwater Hydrology Model (DCHM) forced by hourly atmospheric fields and QPFs (Quantitative Precipitation Forecasts) produced by the NASA-Unified Weather Research and Forecasting (NU-WRF) model. Previous day hindcasts forced by radar-based QPEs (Quantitative Precipitation Estimates) were used to provide initial conditions for present day forecasts. This manuscript first describes the operational testbed framework and workflow during the IPHEx-IOP including a synthesis of results. Second, various data assimilation approaches are explored a posteriori (post-IOP) to improve operational (flash) flood forecasting. Although all flood events during the IOP were predicted by the IPHEx operational testbed with lead times of up to 6 h, significant errors of over- and, or under-prediction were identified that could be traced back to the QPFs and subgrid-scale variability of radar QPEs. To improve operational flood prediction, three data-merging strategies were pursued post-IOP: (1) the spatial patterns of QPFs were improved through assimilation of satellite-based microwave radiances into NU-WRF; (2) QPEs were improved by merging raingauge observations with ground-based radar observations using bias-correction methods to produce streamflow hindcasts and associated uncertainty envelope capturing the streamflow observations, and (3) river discharge observations were assimilated into the DCHM to improve streamflow forecasts using the Ensemble Kalman Filter (EnKF), the fixed-lag Ensemble Kalman Smoother (EnKS), and the Asynchronous EnKF (i.e. AEnKF) methods. Both flood hindcasts and forecasts

  17. PREFACE: The Eighth Liquid Matter Conference The Eighth Liquid Matter Conference

    NASA Astrophysics Data System (ADS)

    Dellago, Christoph; Kahl, Gerhard; Likos, Christos N.

    2012-07-01

    interface tension of flat and curved interfaces from Monte Carlo simulationsA Tröster and K Binder Phase diagrams of particles with dissimilar patches: X-junctions and Y-junctionsJ M Tavares and P I C Teixeira The unbearable heaviness of colloids: facts, surprises, and puzzles in sedimentationRoberto Piazza, Stefano Buzzaccaro and Eleonora Secchi Exploring water and other liquids at negative pressureFrédéric Caupin, Arnaud Arvengas, Kristina Davitt, Mouna El Mekki Azouzi, Kirill I Shmulovich, Claire Ramboz, David A Sessoms and Abraham D Stroock The configurational space of colloidal patchy polymers with heterogeneous sequencesIvan Coluzza and Christoph Dellago Repeated sorption of water in SBA-15 investigated by means of in situ small-angle x-ray scatteringM Erko, D Wallacher, G H Findenegg and O Paris Transition of the hydration state of a surfactant accompanying structural transitions of self-assembled aggregatesM Hishida and K Tanaka The effects of topology on the structural, dynamic and mechanical properties of network-forming materialsMark Wilson Surface tension of an electrolyte-air interface: a Monte Carlo studyAlexandre Diehl, Alexandre P dos Santos and Yan Levin Water and other tetrahedral liquids: order, anomalies and solvationB Shadrack Jabes, Divya Nayar, Debdas Dhabal, Valeria Molinero and Charusita Chakravarty Diffusion coefficient and shear viscosity of rigid water modelsSami Tazi, Alexandru Boţan, Mathieu Salanne, Virginie Marry, Pierre Turq and Benjamin Rotenberg Phase behaviour of colloidal assemblies on 2D corrugated substratesSamir El Shawish, Emmanuel Trizac and Jure Dobnikar Structural properties of dendrimer-colloid mixturesDominic A Lenz, Ronald Blaak and Christos N Likos Fluid-fluid demixing of off-critical colloid-polymer systems confined between parallel platesE A G Jamie, R P A Dullens and D G A L Aarts Simulations of nematic homopolymer melts using particle-based models with interactions expressed through collective variablesKostas Ch