Sample records for z-imaging single objects

  1. A method of object recognition for single pixel imaging

    NASA Astrophysics Data System (ADS)

    Li, Boxuan; Zhang, Wenwen

    2018-01-01

    Computational ghost imaging(CGI), utilizing a single-pixel detector, has been extensively used in many fields. However, in order to achieve a high-quality reconstructed image, a large number of iterations are needed, which limits the flexibility of using CGI in practical situations, especially in the field of object recognition. In this paper, we purpose a method utilizing the feature matching to identify the number objects. In the given system, approximately 90% of accuracy of recognition rates can be achieved, which provides a new idea for the application of single pixel imaging in the field of object recognition

  2. Single-pixel non-imaging object recognition by means of Fourier spectrum acquisition

    NASA Astrophysics Data System (ADS)

    Chen, Huichao; Shi, Jianhong; Liu, Xialin; Niu, Zhouzhou; Zeng, Guihua

    2018-04-01

    Single-pixel imaging has emerged over recent years as a novel imaging technique, which has significant application prospects. In this paper, we propose and experimentally demonstrate a scheme that can achieve single-pixel non-imaging object recognition by acquiring the Fourier spectrum. In an experiment, a four-step phase-shifting sinusoid illumination light is used to irradiate the object image, the value of the light intensity is measured with a single-pixel detection unit, and the Fourier coefficients of the object image are obtained by a differential measurement. The Fourier coefficients are first cast into binary numbers to obtain the hash value. We propose a new method of perceptual hashing algorithm, which is combined with a discrete Fourier transform to calculate the hash value. The hash distance is obtained by calculating the difference of the hash value between the object image and the contrast images. By setting an appropriate threshold, the object image can be quickly and accurately recognized. The proposed scheme realizes single-pixel non-imaging perceptual hashing object recognition by using fewer measurements. Our result might open a new path for realizing object recognition with non-imaging.

  3. 3D high- and super-resolution imaging using single-objective SPIM.

    PubMed

    Galland, Remi; Grenci, Gianluca; Aravind, Ajay; Viasnoff, Virgile; Studer, Vincent; Sibarita, Jean-Baptiste

    2015-07-01

    Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger mirrors enabled us to broaden the capabilities of our system to image Drosophila embryos.

  4. Multiplicative mixing of object identity and image attributes in single inferior temporal neurons.

    PubMed

    Ratan Murty, N Apurva; Arun, S P

    2018-04-03

    Object recognition is challenging because the same object can produce vastly different images, mixing signals related to its identity with signals due to its image attributes, such as size, position, rotation, etc. Previous studies have shown that both signals are present in high-level visual areas, but precisely how they are combined has remained unclear. One possibility is that neurons might encode identity and attribute signals multiplicatively so that each can be efficiently decoded without interference from the other. Here, we show that, in high-level visual cortex, responses of single neurons can be explained better as a product rather than a sum of tuning for object identity and tuning for image attributes. This subtle effect in single neurons produced substantially better population decoding of object identity and image attributes in the neural population as a whole. This property was absent both in low-level vision models and in deep neural networks. It was also unique to invariances: when tested with two-part objects, neural responses were explained better as a sum than as a product of part tuning. Taken together, our results indicate that signals requiring separate decoding, such as object identity and image attributes, are combined multiplicatively in IT neurons, whereas signals that require integration (such as parts in an object) are combined additively. Copyright © 2018 the Author(s). Published by PNAS.

  5. Fast Neutron Tomography of Low-Z Object in High-Z Material Shielding

    NASA Astrophysics Data System (ADS)

    Babai, Ruth Weiss; Sabo-Napadensky, Iris; Bar, Doron; Mor, Ilan; Tamim, Noam; Dangendorf, Volker; Tittelmeier, Kai; Bromberger, Benjamin; Weierganz, Mathias

    The technique and first results of Fast Neutron Tomography (FNCT) experiments are presented which are performed at the accelerator facility of PTB, Germany. A high-intensity neutron beam of broad spectral distribution with an average energy of 5.5 MeV, was produced by 11.5 MeV deuterons impinging upon a thick beryllium target. The capability of FNCT for high contrast imaging of low-Z materials embedded in thick high-Z shielding materials is demonstrated, which is superior to more conventional high-energy X-ray imaging techniques. For demonstrating the method special test objects were prepared: One consisted of an assembled polyethylene cylinder with holes of various diameters and directions drilled in its surface and inner parts. The plastic phantom was inserted into lead cylinders of different thicknesses. The detector system consisted of a plastic scintillator along with a dedicated optics, image-intensifier and a CCD camera. Two scintillator screens were compared: a bulk plastic scintillator screen and a fibres optical scintillator screen. The tomographic scans were taken in two geometrical configurations: cone beam and semi-fan beam configuration. The image quality favours the semi-fan beam configuration which on the other hand is more time consuming The obtained tomographic images and a comparison of the imaging quality between the different experimental conditions will be presented.

  6. Color evolution from z = 0 to z = 1

    NASA Technical Reports Server (NTRS)

    Rakos, Karl D.; Schombert, James M.

    1995-01-01

    Rest frame Stroemgren photometry (3500 A, 4100 A, 4750 A, and 5500 A) is presented for 509 galaxies in 17 rich clusters between z = 0 and z = 1 as a test of color evolution. Our observations confirm a strong, rest frame, Butcher-Oemler effect where the fraction of blue galaxies increases from 20% at z = 0.4 to 80% at z = 0.9. We also find that a majority of these blue cluster galaxies are composed of normal disk or post-starburst systems based on color criteria. When comparing our colors to the morphological results from Hubble Space Telescope HST imaging, we propose that the blue cluster galaxies are a population of late-type, low surface brightness objects which fade and are then destroyed by the cluster tidal field. After isolating the red objects from Butcher-Oemler objects, we have compared the mean color of these old, non-star-forming objects with spectral energy distribution models in the literature as a test for passive galaxy evolution in ellipticals. We find good agreement with single-burst models which predict a mean epoch of galaxy formation at z = 5. Tracing the red envelope for ellipticals places the earliest epoch of galaxy formation at z = 10.

  7. Pseudo-shading technique in the two-dimensional domain: a post-processing algorithm for enhancing the Z-buffer of a three-dimensional binary image.

    PubMed

    Tan, A C; Richards, R

    1989-01-01

    Three-dimensional (3D) medical graphics is becoming popular in clinical use on tomographic scanners. Research work in 3D reconstructive display of computerized tomography (CT) and magnetic resonance imaging (MRI) scans on conventional computers has produced many so-called pseudo-3D images. The quality of these images depends on the rendering algorithm, the coarseness of the digitized object, the number of grey levels and the image screen resolution. CT and MRI data are fundamentally voxel based and they produce images that are coarse because of the resolution of the data acquisition system. 3D images produced by the Z-buffer depth shading technique suffer loss of detail when complex objects with fine textural detail need to be displayed. Attempts have been made to improve the display of voxel objects, and existing techniques have shown the improvement possible using these post-processing algorithms. The improved rendering technique works on the Z-buffer image to generate a shaded image using a single light source in any direction. The effectiveness of the technique in generating a shaded image has been shown to be a useful means of presenting 3D information for clinical use.

  8. Noninvasive, near-field terahertz imaging of hidden objects using a single-pixel detector.

    PubMed

    Stantchev, Rayko Ivanov; Sun, Baoqing; Hornett, Sam M; Hobson, Peter A; Gibson, Graham M; Padgett, Miles J; Hendry, Euan

    2016-06-01

    Terahertz (THz) imaging can see through otherwise opaque materials. However, because of the long wavelengths of THz radiation (λ = 400 μm at 0.75 THz), far-field THz imaging techniques suffer from low resolution compared to visible wavelengths. We demonstrate noninvasive, near-field THz imaging with subwavelength resolution. We project a time-varying, intense (>100 μJ/cm(2)) optical pattern onto a silicon wafer, which spatially modulates the transmission of synchronous pulse of THz radiation. An unknown object is placed on the hidden side of the silicon, and the far-field THz transmission corresponding to each mask is recorded by a single-element detector. Knowledge of the patterns and of the corresponding detector signal are combined to give an image of the object. Using this technique, we image a printed circuit board on the underside of a 115-μm-thick silicon wafer with ~100-μm (λ/4) resolution. With subwavelength resolution and the inherent sensitivity to local conductivity, it is possible to detect fissures in the circuitry wiring of a few micrometers in size. THz imaging systems of this type will have other uses too, where noninvasive measurement or imaging of concealed structures is necessary, such as in semiconductor manufacturing or in ex vivo bioimaging.

  9. Determining Object Orientation from a Single Image Using Multiple Information Sources.

    DTIC Science & Technology

    1984-06-01

    object surface. Location of the image ellipse is accomplished by exploiting knowledge about object boundaries and image intensity gradients . -. The...Using Intensity Gradient Information for Ellipse fitting ........ .51 4.3.7 Orientation From Ellipses .............................. 53 4.3.8 Application...object boundaries and image intensity gradients . The orientation information from each of these three methods is combined using a "plausibility" function

  10. Incoherent coincidence imaging of space objects

    NASA Astrophysics Data System (ADS)

    Mao, Tianyi; Chen, Qian; He, Weiji; Gu, Guohua

    2016-10-01

    Incoherent Coincidence Imaging (ICI), which is based on the second or higher order correlation of fluctuating light field, has provided great potentialities with respect to standard conventional imaging. However, the deployment of reference arm limits its practical applications in the detection of space objects. In this article, an optical aperture synthesis with electronically connected single-pixel photo-detectors was proposed to remove the reference arm. The correlation in our proposed method is the second order correlation between the intensity fluctuations observed by any two detectors. With appropriate locations of single-pixel detectors, this second order correlation is simplified to absolute-square Fourier transform of source and the unknown object. We demonstrate the image recovery with the Gerchberg-Saxton-like algorithms and investigate the reconstruction quality of our approach. Numerical experiments has been made to show that both binary and gray-scale objects can be recovered. This proposed method provides an effective approach to promote detection of space objects and perhaps even the exo-planets.

  11. Vigorous star formation in a bulge-dominated extremely red object at z= 1.34

    NASA Astrophysics Data System (ADS)

    Cotter, Garret; Simpson, Chris; Bolton, Rosemary C.

    2005-06-01

    We present near-infrared (near-IR) spectroscopy of three extremely red objects (EROs) using the OHS/CISCO spectrograph at the Subaru Telescope. One target exhibits a strong emission line, which we identify as Hα at z= 1.34. Using new and existing ground-based optical and near-IR imaging, and archival Hubble Space Telescope imaging, we argue that this target is essentially an elliptical galaxy, with an old stellar population of around 4 × 1011Msolar, but having a dust-enshrouded star-forming component with a star formation rate (SFR) of some 50-100Msolar yr-1. There is no evidence that the galaxy contains an active galactic nucleus. Analysis of a further two targets, which do not exhibit any features in our near-IR spectra, suggests that one is a quiescent galaxy in the redshift range 1.2 < z < 1.6, but that the other cannot be conclusively categorized as either star-forming or quiescent. Even though our first target has many of the properties of an old elliptical, the ongoing star formation means that it cannot have formed all of its stellar population at high redshift. While we cannot infer any robust values for the SFR in ellipticals at z > 1 from this one object, we argue that the presence of an object with such a high SFR in such a small sample suggests that a non-negligible fraction of the elliptical galaxy population may have formed a component of their stellar population at redshifts z~ 1-2. We suggest that this is evidence for ongoing star formation in the history of elliptical galaxies.

  12. FAST TRACK COMMUNICATION Far-field x-ray phase contrast imaging has no detailed information on the object

    NASA Astrophysics Data System (ADS)

    Kohn, V. G.; Argunova, T. S.; Je, J. H.

    2010-11-01

    We show that x-ray phase contrast images of some objects with a small cross-section diameter d satisfy a condition for a far-field approximation d Lt r1 where r1 = (λz)1/2, λ is the x-ray wavelength, z is the distance from the object to the detector. In this case the size of the image does not match the size of the object contrary to the edge detection technique. Moreover, the structure of the central fringes of the image is universal, i.e. it is independent of the object cross-section structure. Therefore, these images have no detailed information on the object.

  13. Single-shot and single-sensor high/super-resolution microwave imaging based on metasurface.

    PubMed

    Wang, Libo; Li, Lianlin; Li, Yunbo; Zhang, Hao Chi; Cui, Tie Jun

    2016-06-01

    Real-time high-resolution (including super-resolution) imaging with low-cost hardware is a long sought-after goal in various imaging applications. Here, we propose broadband single-shot and single-sensor high-/super-resolution imaging by using a spatio-temporal dispersive metasurface and an imaging reconstruction algorithm. The metasurface with spatio-temporal dispersive property ensures the feasibility of the single-shot and single-sensor imager for super- and high-resolution imaging, since it can convert efficiently the detailed spatial information of the probed object into one-dimensional time- or frequency-dependent signal acquired by a single sensor fixed in the far-field region. The imaging quality can be improved by applying a feature-enhanced reconstruction algorithm in post-processing, and the desired imaging resolution is related to the distance between the object and metasurface. When the object is placed in the vicinity of the metasurface, the super-resolution imaging can be realized. The proposed imaging methodology provides a unique means to perform real-time data acquisition, high-/super-resolution images without employing expensive hardware (e.g. mechanical scanner, antenna array, etc.). We expect that this methodology could make potential breakthroughs in the areas of microwave, terahertz, optical, and even ultrasound imaging.

  14. Single-shot and single-sensor high/super-resolution microwave imaging based on metasurface

    PubMed Central

    Wang, Libo; Li, Lianlin; Li, Yunbo; Zhang, Hao Chi; Cui, Tie Jun

    2016-01-01

    Real-time high-resolution (including super-resolution) imaging with low-cost hardware is a long sought-after goal in various imaging applications. Here, we propose broadband single-shot and single-sensor high-/super-resolution imaging by using a spatio-temporal dispersive metasurface and an imaging reconstruction algorithm. The metasurface with spatio-temporal dispersive property ensures the feasibility of the single-shot and single-sensor imager for super- and high-resolution imaging, since it can convert efficiently the detailed spatial information of the probed object into one-dimensional time- or frequency-dependent signal acquired by a single sensor fixed in the far-field region. The imaging quality can be improved by applying a feature-enhanced reconstruction algorithm in post-processing, and the desired imaging resolution is related to the distance between the object and metasurface. When the object is placed in the vicinity of the metasurface, the super-resolution imaging can be realized. The proposed imaging methodology provides a unique means to perform real-time data acquisition, high-/super-resolution images without employing expensive hardware (e.g. mechanical scanner, antenna array, etc.). We expect that this methodology could make potential breakthroughs in the areas of microwave, terahertz, optical, and even ultrasound imaging. PMID:27246668

  15. Comparison of image quality and radiation dose between split-filter dual-energy images and single-energy images in single-source abdominal CT.

    PubMed

    Euler, André; Obmann, Markus M; Szucs-Farkas, Zsolt; Mileto, Achille; Zaehringer, Caroline; Falkowski, Anna L; Winkel, David J; Marin, Daniele; Stieltjes, Bram; Krauss, Bernhard; Schindera, Sebastian T

    2018-02-19

    To compare image quality and radiation dose of abdominal split-filter dual-energy CT (SF-DECT) combined with monoenergetic imaging to single-energy CT (SECT) with automatic tube voltage selection (ATVS). Two-hundred single-source abdominal CT scans were performed as SECT with ATVS (n = 100) and SF-DECT (n = 100). SF-DECT scans were reconstructed and subdivided into composed images (SF-CI) and monoenergetic images at 55 keV (SF-MI). Objective and subjective image quality were compared among single-energy images (SEI), SF-CI and SF-MI. CNR and FOM were separately calculated for the liver (e.g. CNR liv ) and the portal vein (CNR pv ). Radiation dose was compared using size-specific dose estimate (SSDE). Results of the three groups were compared using non-parametric tests. Image noise of SF-CI was 18% lower compared to SEI and 48% lower compared to SF-MI (p < 0.001). Composed images yielded higher CNR liv over single-energy images (23.4 vs. 20.9; p < 0.001), whereas CNR pv was significantly lower (3.5 vs. 5.2; p < 0.001). Monoenergetic images overcame this inferiority in CNR pv and achieved similar results compared to single-energy images (5.1 vs. 5.2; p > 0.628). Subjective sharpness was equal between single-energy and monoenergetic images and diagnostic confidence was equal between single-energy and composed images. FOM liv was highest for SF-CI. FOM pv was equal for SEI and SF-MI (p = 0.78). SSDE was significant lower for SF-DECT compared to SECT (p < 0.022). The combined use of split-filter dual-energy CT images provides comparable objective and subjective image quality at lower radiation dose compared to single-energy CT with ATVS. • Split-filter dual-energy results in 18% lower noise compared to single-energy with ATVS. • Split-filter dual-energy results in 11% lower SSDE compared to single-energy with ATVS. • Spectral shaping of split-filter dual-energy leads to an increased dose-efficiency.

  16. Single-particle mineralogy of Chinese soil particles by the combined use of low-Z particle electron probe X-ray microanalysis and attenuated total reflectance-FT-IR imaging techniques.

    PubMed

    Malek, Md Abdul; Kim, Bowha; Jung, Hae-Jin; Song, Young-Chul; Ro, Chul-Un

    2011-10-15

    Our previous work on the speciation of individual mineral particles of micrometer size by the combined use of attenuated total reflectance FT-IR (ATR-FT-IR) imaging and a quantitative energy-dispersive electron probe X-ray microanalysis technique (EPMA), low-Z particle EPMA, demonstrated that the combined use of these two techniques is a powerful approach for looking at the single-particle mineralogy of externally heterogeneous minerals. In this work, this analytical methodology was applied to characterize six soil samples collected at arid areas in China, in order to identify mineral types present in the samples. The six soil samples were collected from two types of soil, i.e., loess and desert soils, for which overall 665 particles were analyzed on a single particle basis. The six soil samples have different mineralogical characteristics, which were clearly differentiated in this work. As this analytical methodology provides complementary information, the ATR-FT-IR imaging on mineral types, and low-Z particle EPMA on the morphology and elemental concentrations, on the same individual particles, more detailed information can be obtained using this approach than when either low-Z particle EPMA or ATR-FT-IR imaging techniques are used alone, which has a great potential for the characterization of Asian dust and mineral dust particles. © 2011 American Chemical Society

  17. Single-Molecule and Superresolution Imaging in Live Bacteria Cells

    PubMed Central

    Biteen, Julie S.; Moerner, W.E.

    2010-01-01

    Single-molecule imaging enables biophysical measurements devoid of ensemble averaging, gives enhanced spatial resolution beyond the diffraction limit, and permits superresolution reconstructions. Here, single-molecule and superresolution imaging are applied to the study of proteins in live Caulobacter crescentus cells to illustrate the power of these methods in bacterial imaging. Based on these techniques, the diffusion coefficient and dynamics of the histidine protein kinase PleC, the localization behavior of the polar protein PopZ, and the treadmilling behavior and protein superstructure of the structural protein MreB are investigated with sub-40-nm spatial resolution, all in live cells. PMID:20300204

  18. Single-shot Z(eff) dense plasma diagnostic through simultaneous refraction and attenuation measurements with a Talbot-Lau x-ray moiré deflectometer.

    PubMed

    Valdivia, M P; Stutman, D; Finkenthal, M

    2015-04-01

    The Talbot-Lau x-ray moiré deflectometer is a powerful plasma diagnostic capable of delivering simultaneous refraction and attenuation information through the accurate detection of x-ray phase shift and intensity. The diagnostic can provide the index of refraction n=1-δ+iβ of an object (dense plasma, for example) placed in the x-ray beam by independently measuring both δ and β, which are directly related to the electron density n(e) and the attenuation coefficient μ, respectively. Since δ and β depend on the effective atomic number Z(eff), a map can be obtained from the ratio between phase and absorption images acquired in a single shot. The Talbot-Lau x-ray moiré deflectometer and its corresponding data acquisition and processing are briefly described to illustrate how the above is achieved; Z(eff) values of test objects within the 4-12 range were obtained experimentally through simultaneous refraction and attenuation measurements. We show that Z(eff) mapping of objects does not require previous knowledge of sample length or shape. The determination of Z(eff) from refraction and attenuation measurements with moiré deflectometry could be of high interest to various domains of high energy density research, such as shocked materials and inertial confinement fusion experiments, as well as material science and nondestructive testing.

  19. Single-shot Z eff dense plasma diagnostic through simultaneous refraction and attenuation measurements with a Talbot–Lau x-ray moiré deflectometer

    DOE PAGES

    Valdivia, M. P.; Stutman, D.; Finkenthal, M.

    2015-03-23

    The Talbot–Lau x-ray moiré deflectometer is a powerful plasma diagnostic capable of delivering simultaneous refraction and attenuation information through the accurate detection of x-ray phase shift and intensity. The diagnostic can provide the index of refraction n=1₋δ + iβ of an object (dense plasma, for example) placed in the x-ray beam by independently measuring both δ and β, which are directly related to the electron density n e and the attenuation coefficient μ respectively. Since δ and β depend on the effective atomic number Z eff, a map can be obtained from the ratio between phase and absorption images acquiredmore » in a single shot. The Talbot–Lau x-ray moiré deflectometer and its corresponding data acquisition and processing are briefly described to illustrate how the above is achieved; Z eff values of test objects within the 4₋12 range were obtained experimentally through simultaneous refraction and attenuation measurements. We show that Z eff mapping of objects does not require previous knowledge of sample length or shape. In conclusion, the determination of Z eff from refraction and attenuation measurements with moiré deflectometry could be of high interest to various domains of high energy density research, such as shocked materials and inertial confinement fusion experiments, as well as material science and nondestructive testing.« less

  20. Single-shot Z eff dense plasma diagnostic through simultaneous refraction and attenuation measurements with a Talbot–Lau x-ray moiré deflectometer

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Valdivia, M. P.; Stutman, D.; Finkenthal, M.

    The Talbot–Lau x-ray moiré deflectometer is a powerful plasma diagnostic capable of delivering simultaneous refraction and attenuation information through the accurate detection of x-ray phase shift and intensity. The diagnostic can provide the index of refraction n=1₋δ + iβ of an object (dense plasma, for example) placed in the x-ray beam by independently measuring both δ and β, which are directly related to the electron density n e and the attenuation coefficient μ respectively. Since δ and β depend on the effective atomic number Z eff, a map can be obtained from the ratio between phase and absorption images acquiredmore » in a single shot. The Talbot–Lau x-ray moiré deflectometer and its corresponding data acquisition and processing are briefly described to illustrate how the above is achieved; Z eff values of test objects within the 4₋12 range were obtained experimentally through simultaneous refraction and attenuation measurements. We show that Z eff mapping of objects does not require previous knowledge of sample length or shape. In conclusion, the determination of Z eff from refraction and attenuation measurements with moiré deflectometry could be of high interest to various domains of high energy density research, such as shocked materials and inertial confinement fusion experiments, as well as material science and nondestructive testing.« less

  1. Giga-z: A 100,000 Object Superconducting Spectrophotometer for LSST Follow-up

    NASA Astrophysics Data System (ADS)

    Marsden, Danica W.; Mazin, Benjamin A.; O'Brien, Kieran; Hirata, Chris

    2013-09-01

    We simulate the performance of a new type of instrument, a Superconducting Multi-Object Spectrograph (SuperMOS), that uses microwave kinetic inductance detectors (MKIDs). MKIDs, a new detector technology, feature good quantum efficiency in the UVOIR, can count individual photons with microsecond timing accuracy, and, like X-ray calorimeters, determine their energy to several percent. The performance of Giga-z, a SuperMOS designed for wide field imaging follow-up observations, is evaluated using simulated observations of the COSMOS mock catalog with an array of 100,000 R 423 nm = E/ΔE = 30 MKID pixels. We compare our results against a simultaneous simulation of LSST observations. In 3 yr on a dedicated 4 m class telescope, Giga-z could observe ≈2 billion galaxies, yielding a low-resolution spectral energy distribution spanning 350-1350 nm for each; 1000 times the number measured with any currently proposed LSST spectroscopic follow-up, at a fraction of the cost and time. Giga-z would provide redshifts for galaxies up to z ≈ 6 with magnitudes mi <~ 25, with accuracy σΔz/(1 + z) ≈ 0.03 for the whole sample, and σΔz/(1 + z) ≈ 0.007 for a select subset. We also find catastrophic failure rates and biases that are consistently lower than for LSST. The added constraint on dark energy parameters for WL + CMB by Giga-z using the FoMSWG default model is equivalent to multiplying the LSST Fisher matrix by a factor of α = 1.27 (wp ), 1.53 (wa ), or 1.98 (Δγ). This is equivalent to multiplying both the LSST coverage area and the training sets by α and reducing all systematics by a factor of 1/\\sqrt{\\alpha }, advantages that are robust to even more extreme models of intrinsic alignment.

  2. Super-resolution imaging applied to moving object tracking

    NASA Astrophysics Data System (ADS)

    Swalaganata, Galandaru; Ratna Sulistyaningrum, Dwi; Setiyono, Budi

    2017-10-01

    Moving object tracking in a video is a method used to detect and analyze changes that occur in an object that being observed. Visual quality and the precision of the tracked target are highly wished in modern tracking system. The fact that the tracked object does not always seem clear causes the tracking result less precise. The reasons are low quality video, system noise, small object, and other factors. In order to improve the precision of the tracked object especially for small object, we propose a two step solution that integrates a super-resolution technique into tracking approach. First step is super-resolution imaging applied into frame sequences. This step was done by cropping the frame in several frame or all of frame. Second step is tracking the result of super-resolution images. Super-resolution image is a technique to obtain high-resolution images from low-resolution images. In this research single frame super-resolution technique is proposed for tracking approach. Single frame super-resolution was a kind of super-resolution that it has the advantage of fast computation time. The method used for tracking is Camshift. The advantages of Camshift was simple calculation based on HSV color that use its histogram for some condition and color of the object varies. The computational complexity and large memory requirements required for the implementation of super-resolution and tracking were reduced and the precision of the tracked target was good. Experiment showed that integrate a super-resolution imaging into tracking technique can track the object precisely with various background, shape changes of the object, and in a good light conditions.

  3. Woodland Mapping at Single-Tree Levels Using Object-Oriented Classification of Unmanned Aerial Vehicle (uav) Images

    NASA Astrophysics Data System (ADS)

    Chenari, A.; Erfanifard, Y.; Dehghani, M.; Pourghasemi, H. R.

    2017-09-01

    Remotely sensed datasets offer a reliable means to precisely estimate biophysical characteristics of individual species sparsely distributed in open woodlands. Moreover, object-oriented classification has exhibited significant advantages over different classification methods for delineation of tree crowns and recognition of species in various types of ecosystems. However, it still is unclear if this widely-used classification method can have its advantages on unmanned aerial vehicle (UAV) digital images for mapping vegetation cover at single-tree levels. In this study, UAV orthoimagery was classified using object-oriented classification method for mapping a part of wild pistachio nature reserve in Zagros open woodlands, Fars Province, Iran. This research focused on recognizing two main species of the study area (i.e., wild pistachio and wild almond) and estimating their mean crown area. The orthoimage of study area was consisted of 1,076 images with spatial resolution of 3.47 cm which was georeferenced using 12 ground control points (RMSE=8 cm) gathered by real-time kinematic (RTK) method. The results showed that the UAV orthoimagery classified by object-oriented method efficiently estimated mean crown area of wild pistachios (52.09±24.67 m2) and wild almonds (3.97±1.69 m2) with no significant difference with their observed values (α=0.05). In addition, the results showed that wild pistachios (accuracy of 0.90 and precision of 0.92) and wild almonds (accuracy of 0.90 and precision of 0.89) were well recognized by image segmentation. In general, we concluded that UAV orthoimagery can efficiently produce precise biophysical data of vegetation stands at single-tree levels, which therefore is suitable for assessment and monitoring open woodlands.

  4. Image Discrimination Models Predict Object Detection in Natural Backgrounds

    NASA Technical Reports Server (NTRS)

    Ahumada, Albert J., Jr.; Rohaly, A. M.; Watson, Andrew B.; Null, Cynthia H. (Technical Monitor)

    1994-01-01

    Object detection involves looking for one of a large set of object sub-images in a large set of background images. Image discrimination models only predict the probability that an observer will detect a difference between two images. In a recent study based on only six different images, we found that discrimination models can predict the relative detectability of objects in those images, suggesting that these simpler models may be useful in some object detection applications. Here we replicate this result using a new, larger set of images. Fifteen images of a vehicle in an other-wise natural setting were altered to remove the vehicle and mixed with the original image in a proportion chosen to make the target neither perfectly recognizable nor unrecognizable. The target was also rotated about a vertical axis through its center and mixed with the background. Sixteen observers rated these 30 target images and the 15 background-only images for the presence of a vehicle. The likelihoods of the observer responses were computed from a Thurstone scaling model with the assumption that the detectabilities are proportional to the predictions of an image discrimination model. Three image discrimination models were used: a cortex transform model, a single channel model with a contrast sensitivity function filter, and the Root-Mean-Square (RMS) difference of the digital target and background-only images. As in the previous study, the cortex transform model performed best; the RMS difference predictor was second best; and last, but still a reasonable predictor, was the single channel model. Image discrimination models can predict the relative detectabilities of objects in natural backgrounds.

  5. Using turbulence scintillation to assist object ranging from a single camera viewpoint.

    PubMed

    Wu, Chensheng; Ko, Jonathan; Coffaro, Joseph; Paulson, Daniel A; Rzasa, John R; Andrews, Larry C; Phillips, Ronald L; Crabbs, Robert; Davis, Christopher C

    2018-03-20

    Image distortions caused by atmospheric turbulence are often treated as unwanted noise or errors in many image processing studies. Our study, however, shows that in certain scenarios the turbulence distortion can be very helpful in enhancing image processing results. This paper describes a novel approach that uses the scintillation traits recorded on a video clip to perform object ranging with reasonable accuracy from a single camera viewpoint. Conventionally, a single camera would be confused by the perspective viewing problem, where a large object far away looks the same as a small object close by. When the atmospheric turbulence phenomenon is considered, the edge or texture pixels of an object tend to scintillate and vary more with increased distance. This turbulence induced signature can be quantitatively analyzed to achieve object ranging with reasonable accuracy. Despite the inevitable fact that turbulence will cause random blurring and deformation of imaging results, it also offers convenient solutions to some remote sensing and machine vision problems, which would otherwise be difficult.

  6. Atomic resolution Z-contrast imaging and energy loss spectroscopy of carbon nanotubes and bundles

    NASA Astrophysics Data System (ADS)

    Lupini, A. R.; Chisholm, M. F.; Puretzky, A. A.; Eres, G.; Melechko, A. V.; Schaaff, G.; Lowndes, D. H.; Geohegan, D. B.; Schittenhelm, H.; Pennycook, S. J.; Wang, Y.; Smalley, R. E.

    2002-03-01

    Single-wall carbon nanotubes and bundles were studied by a combination of techniques, including conventional imaging and diffraction, atomic resolution Z-contrast imaging in an aberration corrected STEM and electron energy loss spectroscopy (EELS). EELS is ideally suited for the analysis of carbon based structures because of the ability to distinguish between the different forms, specifically nanotubes, graphite, amorphous carbon and diamond. Numerous attempts were made to synthesize crystals of single walled carbon nanotubes, using both solution and vapor deposition of precursor structures directly onto TEM grids for in-situ annealing. The range of structures produced will be discussed.

  7. Color Image Processing and Object Tracking System

    NASA Technical Reports Server (NTRS)

    Klimek, Robert B.; Wright, Ted W.; Sielken, Robert S.

    1996-01-01

    This report describes a personal computer based system for automatic and semiautomatic tracking of objects on film or video tape, developed to meet the needs of the Microgravity Combustion and Fluids Science Research Programs at the NASA Lewis Research Center. The system consists of individual hardware components working under computer control to achieve a high degree of automation. The most important hardware components include 16-mm and 35-mm film transports, a high resolution digital camera mounted on a x-y-z micro-positioning stage, an S-VHS tapedeck, an Hi8 tapedeck, video laserdisk, and a framegrabber. All of the image input devices are remotely controlled by a computer. Software was developed to integrate the overall operation of the system including device frame incrementation, grabbing of image frames, image processing of the object's neighborhood, locating the position of the object being tracked, and storing the coordinates in a file. This process is performed repeatedly until the last frame is reached. Several different tracking methods are supported. To illustrate the process, two representative applications of the system are described. These applications represent typical uses of the system and include tracking the propagation of a flame front and tracking the movement of a liquid-gas interface with extremely poor visibility.

  8. Giga-z: A 100,000 OBJECT SUPERCONDUCTING SPECTROPHOTOMETER FOR LSST FOLLOW-UP

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Marsden, Danica W.; Mazin, Benjamin A.; O'Brien, Kieran

    2013-09-15

    We simulate the performance of a new type of instrument, a Superconducting Multi-Object Spectrograph (SuperMOS), that uses microwave kinetic inductance detectors (MKIDs). MKIDs, a new detector technology, feature good quantum efficiency in the UVOIR, can count individual photons with microsecond timing accuracy, and, like X-ray calorimeters, determine their energy to several percent. The performance of Giga-z, a SuperMOS designed for wide field imaging follow-up observations, is evaluated using simulated observations of the COSMOS mock catalog with an array of 100,000 R{sub 423{sub nm}} = E/{Delta}E = 30 MKID pixels. We compare our results against a simultaneous simulation of LSST observations.more » In 3 yr on a dedicated 4 m class telescope, Giga-z could observe Almost-Equal-To 2 billion galaxies, yielding a low-resolution spectral energy distribution spanning 350-1350 nm for each; 1000 times the number measured with any currently proposed LSST spectroscopic follow-up, at a fraction of the cost and time. Giga-z would provide redshifts for galaxies up to z Almost-Equal-To 6 with magnitudes m{sub i} {approx}< 25, with accuracy {sigma}{sub {Delta}z/(1+z)} Almost-Equal-To 0.03 for the whole sample, and {sigma}{sub {Delta}z/(1+z)} Almost-Equal-To 0.007 for a select subset. We also find catastrophic failure rates and biases that are consistently lower than for LSST. The added constraint on dark energy parameters for WL + CMB by Giga-z using the FoMSWG default model is equivalent to multiplying the LSST Fisher matrix by a factor of {alpha} = 1.27 (w{sub p} ), 1.53 (w{sub a} ), or 1.98 ({Delta}{gamma}). This is equivalent to multiplying both the LSST coverage area and the training sets by {alpha} and reducing all systematics by a factor of 1/{radical}({alpha}), advantages that are robust to even more extreme models of intrinsic alignment.« less

  9. New insights into FtsZ rearrangements during the cell division of Escherichia coli from single-molecule localization microscopy of fixed cells.

    PubMed

    Vedyaykin, Alexey D; Vishnyakov, Innokentii E; Polinovskaya, Vasilisa S; Khodorkovskii, Mikhail A; Sabantsev, Anton V

    2016-06-01

    FtsZ - a prokaryotic tubulin homolog - is one of the central components of bacterial division machinery. At the early stage of cytokinesis FtsZ forms the so-called Z-ring at mid-cell that guides septum formation. Many approaches were used to resolve the structure of the Z-ring, however, researchers are still far from consensus on this question. We utilized single-molecule localization microscopy (SMLM) in combination with immunofluorescence staining to visualize FtsZ in Esherichia coli fixed cells that were grown under slow and fast growth conditions. This approach allowed us to obtain images of FtsZ structures at different stages of cell division and accurately measure Z-ring dimensions. Analysis of these images demonstrated that Z-ring thickness increases during constriction, starting at about 70 nm at the beginning of division and increasing by approximately 25% half-way through constriction. © 2016 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

  10. Coherent diffraction imaging of non-isolated object with apodized illumination.

    PubMed

    Khakurel, Krishna P; Kimura, Takashi; Joti, Yasumasa; Matsuyama, Satoshi; Yamauchi, Kazuto; Nishino, Yoshinori

    2015-11-02

    Coherent diffraction imaging (CDI) is an established lensless imaging method widely used at the x-ray regime applicable to the imaging of non-periodic materials. Conventional CDI can practically image isolated objects only, which hinders the broader application of the method. We present the imaging of non-isolated objects by employing recently proposed "non-scanning" apodized-illumination CDI at an optical wavelength. We realized isolated apodized illumination with a specially designed optical configuration and succeeded in imaging phase objects as well as amplitude objects. The non-scanning nature of the method is important particularly in imaging live cells and tissues, where fast imaging is required for non-isolated objects, and is an advantage over ptychography. We believe that our result of phase contrast imaging at an optical wavelength can be extended to the quantitative phase imaging of cells and tissues. The method also provides the feasibility of the lensless single-shot imaging of extended objects with x-ray free-electron lasers.

  11. Imaging through scattering media by Fourier filtering and single-pixel detection

    NASA Astrophysics Data System (ADS)

    Jauregui-Sánchez, Y.; Clemente, P.; Lancis, J.; Tajahuerce, E.

    2018-02-01

    We present a novel imaging system that combines the principles of Fourier spatial filtering and single-pixel imaging in order to recover images of an object hidden behind a turbid medium by transillumination. We compare the performance of our single-pixel imaging setup with that of a conventional system. We conclude that the introduction of Fourier gating improves the contrast of images in both cases. Furthermore, we show that the combination of single-pixel imaging and Fourier spatial filtering techniques is particularly well adapted to provide images of objects transmitted through scattering media.

  12. Combining features from ERP components in single-trial EEG for discriminating four-category visual objects.

    PubMed

    Wang, Changming; Xiong, Shi; Hu, Xiaoping; Yao, Li; Zhang, Jiacai

    2012-10-01

    Categorization of images containing visual objects can be successfully recognized using single-trial electroencephalograph (EEG) measured when subjects view images. Previous studies have shown that task-related information contained in event-related potential (ERP) components could discriminate two or three categories of object images. In this study, we investigated whether four categories of objects (human faces, buildings, cats and cars) could be mutually discriminated using single-trial EEG data. Here, the EEG waveforms acquired while subjects were viewing four categories of object images were segmented into several ERP components (P1, N1, P2a and P2b), and then Fisher linear discriminant analysis (Fisher-LDA) was used to classify EEG features extracted from ERP components. Firstly, we compared the classification results using features from single ERP components, and identified that the N1 component achieved the highest classification accuracies. Secondly, we discriminated four categories of objects using combining features from multiple ERP components, and showed that combination of ERP components improved four-category classification accuracies by utilizing the complementarity of discriminative information in ERP components. These findings confirmed that four categories of object images could be discriminated with single-trial EEG and could direct us to select effective EEG features for classifying visual objects.

  13. Three-dimensional object recognition based on planar images

    NASA Astrophysics Data System (ADS)

    Mital, Dinesh P.; Teoh, Eam-Khwang; Au, K. C.; Chng, E. K.

    1993-01-01

    This paper presents the development and realization of a robotic vision system for the recognition of 3-dimensional (3-D) objects. The system can recognize a single object from among a group of known regular convex polyhedron objects that is constrained to lie on a calibrated flat platform. The approach adopted comprises a series of image processing operations on a single 2-dimensional (2-D) intensity image to derive an image line drawing. Subsequently, a feature matching technique is employed to determine 2-D spatial correspondences of the image line drawing with the model in the database. Besides its identification ability, the system can also provide important position and orientation information of the recognized object. The system was implemented on an IBM-PC AT machine executing at 8 MHz without the 80287 Maths Co-processor. In our overall performance evaluation based on a 600 recognition cycles test, the system demonstrated an accuracy of above 80% with recognition time well within 10 seconds. The recognition time is, however, indirectly dependent on the number of models in the database. The reliability of the system is also affected by illumination conditions which must be clinically controlled as in any industrial robotic vision system.

  14. Resolving power of diffraction imaging with an objective: a numerical study.

    PubMed

    Wang, Wenjin; Liu, Jing; Lu, Jun Qing; Ding, Junhua; Hu, Xin-Hua

    2017-05-01

    Diffraction imaging in the far-field can detect 3D morphological features of an object for its coherent nature. We describe methods for accurate calculation and analysis of diffraction images of scatterers of single and double spheres by an imaging unit based on microscope objective at non-conjugate positions. A quantitative study of the calculated diffraction imaging in spectral domain has been performed to assess the resolving power of diffraction imaging. It has been shown numerically that with coherent illumination of 532 nm in wavelength the imaging unit can resolve single spheres of 2 μm or larger in diameters and double spheres separated by less than 300 nm between their centers.

  15. EIT image regularization by a new Multi-Objective Simulated Annealing algorithm.

    PubMed

    Castro Martins, Thiago; Sales Guerra Tsuzuki, Marcos

    2015-01-01

    Multi-Objective Optimization can be used to produce regularized Electrical Impedance Tomography (EIT) images where the weight of the regularization term is not known a priori. This paper proposes a novel Multi-Objective Optimization algorithm based on Simulated Annealing tailored for EIT image reconstruction. Images are reconstructed from experimental data and compared with images from other Multi and Single Objective optimization methods. A significant performance enhancement from traditional techniques can be inferred from the results.

  16. A Narrowband Imaging Search for [O III] Emission from Galaxies at z > 3

    NASA Astrophysics Data System (ADS)

    Teplitz, Harry I.; Malkan, Matthew A.; McLean, Ian S.

    1999-03-01

    We present the results of a narrowband survey of quasi-stellar-object (QSO) fields at redshifts that place the [O III] (5007 Å) emission line in the Δλ/λ~1% 2.16 μm filter. We have observed 3 arcmin2 and detected one emission-line candidate object in the field around PC 1109+4642. We discuss the possibilities that this object is a star-forming galaxy at the QSO redshift, zem=3.313, or a Seyfert galaxy. In the former case, we infer a star formation rate of 170 Msolar yr-1 for this K'=21.3 object. The galaxy has a compact but resolved morphology, with an FWHM=0.6" or 4.2 kpc at z=3.313 (H0=50 km s-1 Mpc-1 and q0=0.5). The comoving density of such objects in QSO environments appears to be 0.0033 Mpc-3, marginally lower (<=3 σ) than the density observed for Hα-emitters in absorption-line fields at z~2.5 but similar to the density of Lyman-break galaxies at z~3. If, on the other hand, most of the line emission is [O III] from a Seyfert 2 nucleus at z=3.31, then the high inferred volume density could imply a large evolution in the Seyfert 2 luminosity function from the current epoch. We find the field containing the object to also contain many faint extended objects in the K' image but little significant excess over the expected number-magnitude relation. We discuss the implication of the emission line being a longer wavelength line at a lower redshift.

  17. Detecting the highest redshift (z > 8) quasi-stellar objects in a wide, near-infrared slitless spectroscopic survey

    NASA Astrophysics Data System (ADS)

    Roche, Nathan; Franzetti, Paolo; Garilli, Bianca; Zamorani, Giovanni; Cimatti, Andrea; Rossetti, Emanuel

    2012-02-01

    We investigate the prospects of extending observations of high-redshift quasi-stellar objects (QSOs) from the current z˜ 7 to z > 8 by means of a very wide-area near-infrared slitless spectroscopic survey, considering as an example the planned survey with the European Space Agency's Euclid telescope (scheduled for a 2019 launch). For any QSOs at z > 8.06, the strong Lyman α line will enter the wavelength range of the Euclid Near-Infrared Spectometer and Imaging Photometer (NISP). We perform a detailed simulation of near infrared spectrometer and imaging photometer (Euclid) NISP slitless spectroscopy (with the parameters of the wide survey) in an artificial field containing QSO spectra at all redshifts up to z= 12 and to a faint limit H= 22.5. QSO spectra are represented with a template based on a Sloan Digital Sky Survey composite spectrum, with the added effects of absorption from neutral hydrogen in the intergalactic medium. The spectra extracted from the simulation are analysed with an automated redshift finder, and a detection rate estimated as a function of H magnitude and redshift (defined as the proportion of spectra with both correct redshift measurements and classifications). We show that, as expected, spectroscopic identification of QSOs would reach deeper limits for the redshift ranges where either ? (0.67 < z < 2.05) or Lyman α (z > 8.06) is visible. Furthermore, if photometrically selected z > 8 spectra can be re-examined and refitted to minimize the effects of spectral contamination, the QSO detection rate in the Lyman α window will be increased by an estimated ˜60 per cent and will then be better here than at any other redshift, with an effective limit H≃ 21.5. With an extrapolated rate of QSO evolution, we predict that the Euclid wide (15 000 ?) spectroscopic survey will identify and measure spectroscopic redshifts for a total of 20-35 QSOs at z > 8.06 (reduced slightly to 19-33 if we apply a small correction for missed weak-lined QSOs

  18. 3D imaging with a single-aperture 3-mm objective lens: concept, fabrication, and test

    NASA Astrophysics Data System (ADS)

    Korniski, Ronald; Bae, Sam Y.; Shearn, Michael; Manohara, Harish; Shahinian, Hrayr

    2011-10-01

    There are many advantages to minimally invasive surgery (MIS). An endoscope is the optical system of choice by the surgeon for MIS. The smaller the incision or opening made to perform the surgery, the smaller the optical system needed. For minimally invasive neurological and skull base surgeries the openings are typically 10-mm in diameter (dime sized) or less. The largest outside diameter (OD) endoscope used is 4mm. A significant drawback to endoscopic MIS is that it only provides a monocular view of the surgical site thereby lacking depth information for the surgeon. A stereo view would provide the surgeon instantaneous depth information of the surroundings within the field of view, a significant advantage especially during brain surgery. Providing 3D imaging in an endoscopic objective lens system presents significant challenges because of the tight packaging constraints. This paper presents a promising new technique for endoscopic 3D imaging that uses a single lens system with complementary multi-bandpass filters (CMBFs), and describes the proof-of-concept demonstrations performed to date validating the technique. These demonstrations of the technique have utilized many commercial off-the- shelf (COTS) components including the ones used in the endoscope objective.

  19. 3D Imaging with a Single-Aperture 3-mm Objective Lens: Concept, Fabrication and Test

    NASA Technical Reports Server (NTRS)

    Korniski, Ron; Bae, Sam Y.; Shearn, Mike; Manohara, Harish; Shahinian, Hrayr

    2011-01-01

    There are many advantages to minimally invasive surgery (MIS). An endoscope is the optical system of choice by the surgeon for MIS. The smaller the incision or opening made to perform the surgery, the smaller the optical system needed. For minimally invasive neurological and skull base surgeries the openings are typically 10-mm in diameter (dime sized) or less. The largest outside diameter (OD) endoscope used is 4mm. A significant drawback to endoscopic MIS is that it only provides a monocular view of the surgical site thereby lacking depth information for the surgeon. A stereo view would provide the surgeon instantaneous depth information of the surroundings within the field of view, a significant advantage especially during brain surgery. Providing 3D imaging in an endoscopic objective lens system presents significant challenges because of the tight packaging constraints. This paper presents a promising new technique for endoscopic 3D imaging that uses a single lens system with complementary multi-bandpass filters (CMBFs), and describes the proof-of-concept demonstrations performed to date validating the technique. These demonstrations of the technique have utilized many commercial off-the-shelf (COTS) components including the ones used in the endoscope objective.

  20. Reducing Variability in Orthogonal Reformatted Image Quality Associated With Axial Long-z-Axis CT Angiography.

    PubMed

    Stein, Erica B; Liu, Peter S; Kazerooni, Ella A; Barber, Karen; Davenport, Matthew S

    2016-12-01

    The objective of our study was to reduce variation in image quality of orthogonal reformatted images generated from long-z-axis CT angiography (CTA) studies of the upper and lower extremities. Upper and lower extremity CTA studies were targeted at a single health care system. A correctly performed CTA examination was defined as one that met the following three criteria: Sagittal and coronal reformats were obtained, a high-resolution matrix greater than 512 × 512 was used, and reformatted images were available in a distance-measurable format. Baseline data were collected from February 1, 2014, through September 30, 2014. Corrective actions were implemented during three consecutive plan-do-check-act (PDCA) cycles from October 1, 2014, through July 31, 2015, that addressed human, technical, and systematic variations. A 3-month maintenance period followed in which no intervention was performed. Longitudinal data were analyzed monthly using a statistical process control chart (p-chart). The total number of long-z-axis extremity CTA studies analyzed was as follows: 351 CTA studies were analyzed at baseline, 94 at the first PDCA cycle, 92 at the second PDCA cycle, 114 at the third PDCA cycle, and 138 during the maintenance period. The monthly rate of correctly performed studies ranged from 7% to 51% (mean, 38% ± 13% [SD]) during the baseline period, 32-59% (mean, 46% ± 14%) during the first PDCA cycle, 40-81% (mean, 61% ± 21%) during the second PDCA cycle, and 80-82% (mean, 81% ± 0.9%) during the third PDCA cycle. The monthly rate improved to 90-91% (mean, 91% ± 0.5%) during the maintenance period. The upper and lower control limits of the p-chart were upshifted after the second and third PDCA cycles. Correcting systematic and technical variations led to the greatest improvements in reformat accuracy. Obtaining consistently and correctly reformatted images from long-z-axis CTA studies is achievable using iterative PDCA cycles.

  1. Accurate color synthesis of three-dimensional objects in an image

    NASA Astrophysics Data System (ADS)

    Xin, John H.; Shen, Hui-Liang

    2004-05-01

    Our study deals with color synthesis of a three-dimensional object in an image; i.e., given a single image, a target color can be accurately mapped onto the object such that the color appearance of the synthesized object closely resembles that of the actual one. As it is almost impossible to acquire the complete geometric description of the surfaces of an object in an image, this study attempted to recover the implicit description of geometry for the color synthesis. The description was obtained from either a series of spectral reflectances or the RGB signals at different surface positions on the basis of the dichromatic reflection model. The experimental results showed that this implicit image-based representation is related to the object geometry and is sufficient for accurate color synthesis of three-dimensional objects in an image. The method established is applicable to the color synthesis of both rigid and deformable objects and should contribute to color fidelity in virtual design, manufacturing, and retailing.

  2. Single-shot ultrafast tomographic imaging by spectral multiplexing

    NASA Astrophysics Data System (ADS)

    Matlis, N. H.; Axley, A.; Leemans, W. P.

    2012-10-01

    Computed tomography has profoundly impacted science, medicine and technology by using projection measurements scanned over multiple angles to permit cross-sectional imaging of an object. The application of computed tomography to moving or dynamically varying objects, however, has been limited by the temporal resolution of the technique, which is set by the time required to complete the scan. For objects that vary on ultrafast timescales, traditional scanning methods are not an option. Here we present a non-scanning method capable of resolving structure on femtosecond timescales by using spectral multiplexing of a single laser beam to perform tomographic imaging over a continuous range of angles simultaneously. We use this technique to demonstrate the first single-shot ultrafast computed tomography reconstructions and obtain previously inaccessible structure and position information for laser-induced plasma filaments. This development enables real-time tomographic imaging for ultrafast science, and offers a potential solution to the challenging problem of imaging through scattering surfaces.

  3. Long working distance objective lenses for single atom trapping and imaging

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pritchard, J. D., E-mail: jonathan.pritchard@strath.ac.uk; Department of Physics, University of Strathclyde, 107 Rottenrow East, Glasgow G4 0NG; Isaacs, J. A.

    We present a pair of optimized objective lenses with long working distances of 117 mm and 65 mm, respectively, that offer diffraction limited performance for both Cs and Rb wavelengths when imaging through standard vacuum windows. The designs utilise standard catalog lens elements to provide a simple and cost-effective solution. Objective 1 provides NA = 0.175 offering 3 μm resolution whilst objective 2 is optimized for high collection efficiency with NA = 0.29 and 1.8 μm resolution. This flexible design can be further extended for use at shorter wavelengths by simply re-optimising the lens separations.

  4. Improving z-tracking accuracy in the two-photon single-particle tracking microscope.

    PubMed

    Liu, C; Liu, Y-L; Perillo, E P; Jiang, N; Dunn, A K; Yeh, H-C

    2015-10-12

    Here, we present a method that can improve the z-tracking accuracy of the recently invented TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) microscope. This method utilizes a maximum likelihood estimator (MLE) to determine the particle's 3D position that maximizes the likelihood of the observed time-correlated photon count distribution. Our Monte Carlo simulations show that the MLE-based tracking scheme can improve the z-tracking accuracy of TSUNAMI microscope by 1.7 fold. In addition, MLE is also found to reduce the temporal correlation of the z-tracking error. Taking advantage of the smaller and less temporally correlated z-tracking error, we have precisely recovered the hybridization-melting kinetics of a DNA model system from thousands of short single-particle trajectories in silico . Our method can be generally applied to other 3D single-particle tracking techniques.

  5. Multi-objective vs. single-objective calibration of a hydrologic model using single- and multi-objective screening

    NASA Astrophysics Data System (ADS)

    Mai, Juliane; Cuntz, Matthias; Shafii, Mahyar; Zink, Matthias; Schäfer, David; Thober, Stephan; Samaniego, Luis; Tolson, Bryan

    2016-04-01

    Hydrologic models are traditionally calibrated against observed streamflow. Recent studies have shown however, that only a few global model parameters are constrained using this kind of integral signal. They can be identified using prior screening techniques. Since different objectives might constrain different parameters, it is advisable to use multiple information to calibrate those models. One common approach is to combine these multiple objectives (MO) into one single objective (SO) function and allow the use of a SO optimization algorithm. Another strategy is to consider the different objectives separately and apply a MO Pareto optimization algorithm. In this study, two major research questions will be addressed: 1) How do multi-objective calibrations compare with corresponding single-objective calibrations? 2) How much do calibration results deteriorate when the number of calibrated parameters is reduced by a prior screening technique? The hydrologic model employed in this study is a distributed hydrologic model (mHM) with 52 model parameters, i.e. transfer coefficients. The model uses grid cells as a primary hydrologic unit, and accounts for processes like snow accumulation and melting, soil moisture dynamics, infiltration, surface runoff, evapotranspiration, subsurface storage and discharge generation. The model is applied in three distinct catchments over Europe. The SO calibrations are performed using the Dynamically Dimensioned Search (DDS) algorithm with a fixed budget while the MO calibrations are achieved using the Pareto Dynamically Dimensioned Search (PA-DDS) algorithm allowing for the same budget. The two objectives used here are the Nash Sutcliffe Efficiency (NSE) of the simulated streamflow and the NSE of the logarithmic transformation. It is shown that the SO DDS results are located close to the edges of the Pareto fronts of the PA-DDS. The MO calibrations are hence preferable due to their supply of multiple equivalent solutions from which the

  6. Improving z-tracking accuracy in the two-photon single-particle tracking microscope

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Liu, C.; Liu, Y.-L.; Perillo, E. P.

    Here, we present a method that can improve the z-tracking accuracy of the recently invented TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) microscope. This method utilizes a maximum likelihood estimator (MLE) to determine the particle's 3D position that maximizes the likelihood of the observed time-correlated photon count distribution. Our Monte Carlo simulations show that the MLE-based tracking scheme can improve the z-tracking accuracy of TSUNAMI microscope by 1.7 fold. In addition, MLE is also found to reduce the temporal correlation of the z-tracking error. Taking advantage of the smaller and less temporally correlated z-tracking error, we havemore » precisely recovered the hybridization-melting kinetics of a DNA model system from thousands of short single-particle trajectories in silico. Our method can be generally applied to other 3D single-particle tracking techniques.« less

  7. Transmission-Type 2-Bit Programmable Metasurface for Single-Sensor and Single-Frequency Microwave Imaging.

    PubMed

    Li, Yun Bo; Li, Lian Lin; Xu, Bai Bing; Wu, Wei; Wu, Rui Yuan; Wan, Xiang; Cheng, Qiang; Cui, Tie Jun

    2016-03-30

    The programmable and digital metamaterials or metasurfaces presented recently have huge potentials in designing real-time-controlled electromagnetic devices. Here, we propose the first transmission-type 2-bit programmable coding metasurface for single-sensor and single- frequency imaging in the microwave frequency. Compared with the existing single-sensor imagers composed of active spatial modulators with their units controlled independently, we introduce randomly programmable metasurface to transform the masks of modulators, in which their rows and columns are controlled simultaneously so that the complexity and cost of the imaging system can be reduced drastically. Different from the single-sensor approach using the frequency agility, the proposed imaging system makes use of variable modulators under single frequency, which can avoid the object dispersion. In order to realize the transmission-type 2-bit programmable metasurface, we propose a two-layer binary coding unit, which is convenient for changing the voltages in rows and columns to switch the diodes in the top and bottom layers, respectively. In our imaging measurements, we generate the random codes by computer to achieve different transmission patterns, which can support enough multiple modes to solve the inverse-scattering problem in the single-sensor imaging. Simple experimental results are presented in the microwave frequency, validating our new single-sensor and single-frequency imaging system.

  8. Direct single-shot phase retrieval from the diffraction pattern of separated objects

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Leshem, Ben; Xu, Rui; Dallal, Yehonatan

    The non-crystallographic phase problem arises in numerous scientific and technological fields. An important application is coherent diffractive imaging. Recent advances in X-ray free-electron lasers allow capturing of the diffraction pattern from a single nanoparticle before it disintegrates, in so-called ‘diffraction before destruction’ experiments. Presently, the phase is reconstructed by iterative algorithms, imposing a non-convex computational challenge, or by Fourier holography, requiring a well-characterized reference field. Here we present a convex scheme for single-shot phase retrieval for two (or more) sufficiently separated objects, demonstrated in two dimensions. In our approach, the objects serve as unknown references to one another, reducing themore » phase problem to a solvable set of linear equations. We establish our method numerically and experimentally in the optical domain and demonstrate a proof-of-principle single-shot coherent diffractive imaging using X-ray free-electron lasers pulses. Lastly, our scheme alleviates several limitations of current methods, offering a new pathway towards direct reconstruction of complex objects.« less

  9. Direct single-shot phase retrieval from the diffraction pattern of separated objects

    DOE PAGES

    Leshem, Ben; Xu, Rui; Dallal, Yehonatan; ...

    2016-02-22

    The non-crystallographic phase problem arises in numerous scientific and technological fields. An important application is coherent diffractive imaging. Recent advances in X-ray free-electron lasers allow capturing of the diffraction pattern from a single nanoparticle before it disintegrates, in so-called ‘diffraction before destruction’ experiments. Presently, the phase is reconstructed by iterative algorithms, imposing a non-convex computational challenge, or by Fourier holography, requiring a well-characterized reference field. Here we present a convex scheme for single-shot phase retrieval for two (or more) sufficiently separated objects, demonstrated in two dimensions. In our approach, the objects serve as unknown references to one another, reducing themore » phase problem to a solvable set of linear equations. We establish our method numerically and experimentally in the optical domain and demonstrate a proof-of-principle single-shot coherent diffractive imaging using X-ray free-electron lasers pulses. Lastly, our scheme alleviates several limitations of current methods, offering a new pathway towards direct reconstruction of complex objects.« less

  10. A Ly{alpha} GALAXY AT REDSHIFT z = 6.944 IN THE COSMOS FIELD

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rhoads, James E.; Hibon, Pascale; Malhotra, Sangeeta

    2012-06-20

    Ly{alpha} emitting galaxies can be used to study cosmological reionization, because a neutral intergalactic medium (IGM) scatters Ly{alpha} photons into diffuse halos whose surface brightness falls below typical survey detection limits. Here, we present the Ly{alpha} emitting galaxy LAE J095950.99+021219.1, identified at redshift z = 6.944 in the COSMOS field using narrowband imaging and follow-up spectroscopy with the IMACS instrument on the Magellan I Baade telescope. With a single object spectroscopically confirmed so far, our survey remains consistent with a wide range of IGM neutral fraction at z Almost-Equal-To 7, but further observations are planned and will help clarify themore » situation. Meantime, the object we present here is only the third Ly{alpha}-selected galaxy to be spectroscopically confirmed at z {approx}> 7, and is {approx}2-3 times fainter than the previously confirmed z Almost-Equal-To 7 Ly{alpha} galaxies.« less

  11. Transmission-Type 2-Bit Programmable Metasurface for Single-Sensor and Single-Frequency Microwave Imaging

    PubMed Central

    Li, Yun Bo; Li, Lian Lin; Xu, Bai Bing; Wu, Wei; Wu, Rui Yuan; Wan, Xiang; Cheng, Qiang; Cui, Tie Jun

    2016-01-01

    The programmable and digital metamaterials or metasurfaces presented recently have huge potentials in designing real-time-controlled electromagnetic devices. Here, we propose the first transmission-type 2-bit programmable coding metasurface for single-sensor and single- frequency imaging in the microwave frequency. Compared with the existing single-sensor imagers composed of active spatial modulators with their units controlled independently, we introduce randomly programmable metasurface to transform the masks of modulators, in which their rows and columns are controlled simultaneously so that the complexity and cost of the imaging system can be reduced drastically. Different from the single-sensor approach using the frequency agility, the proposed imaging system makes use of variable modulators under single frequency, which can avoid the object dispersion. In order to realize the transmission-type 2-bit programmable metasurface, we propose a two-layer binary coding unit, which is convenient for changing the voltages in rows and columns to switch the diodes in the top and bottom layers, respectively. In our imaging measurements, we generate the random codes by computer to achieve different transmission patterns, which can support enough multiple modes to solve the inverse-scattering problem in the single-sensor imaging. Simple experimental results are presented in the microwave frequency, validating our new single-sensor and single-frequency imaging system. PMID:27025907

  12. Analyser-based mammography using single-image reconstruction.

    PubMed

    Briedis, Dahliyani; Siu, Karen K W; Paganin, David M; Pavlov, Konstantin M; Lewis, Rob A

    2005-08-07

    We implement an algorithm that is able to decode a single analyser-based x-ray phase-contrast image of a sample, converting it into an equivalent conventional absorption-contrast radiograph. The algorithm assumes the projection approximation for x-ray propagation in a single-material object embedded in a substrate of approximately uniform thickness. Unlike the phase-contrast images, which have both directional bias and a bias towards edges present in the sample, the reconstructed images are directly interpretable in terms of the projected absorption coefficient of the sample. The technique was applied to a Leeds TOR[MAM] phantom, which is designed to test mammogram quality by the inclusion of simulated microcalcifications, filaments and circular discs. This phantom was imaged at varying doses using three modalities: analyser-based synchrotron phase-contrast images converted to equivalent absorption radiographs using our algorithm, slot-scanned synchrotron imaging and imaging using a conventional mammography unit. Features in the resulting images were then assigned a quality score by volunteers. The single-image reconstruction method achieved higher scores at equivalent and lower doses than the conventional mammography images, but no improvement of visualization of the simulated microcalcifications, and some degradation in image quality at reduced doses for filament features.

  13. Imaging the distribution of individual platinum-based anticancer drug molecules attached to single-wall carbon nanotubes

    PubMed Central

    Bhirde, Ashwin A; Sousa, Alioscka A; Patel, Vyomesh; Azari, Afrouz A; Gutkind, J Silvio; Leapman, Richard D; Rusling, James F

    2009-01-01

    Aims To image the distribution of drug molecules attached to single-wall carbon nanotubes (SWNTs). Materials & methods Herein we report the use of scanning transmission electron microscopy (STEM) for atomic scale visualization and quantitation of single platinum-based drug molecules attached to SWNTs designed for targeted drug delivery. Fourier transform infrared spectroscopy and energy-dispersive x-ray spectroscopy were used for characterization of the SWNT drug conjugates. Results Z-contrast STEM imaging enabled visualization of the first-line anticancer drug cisplatin on the nanotubes at single molecule level. The identity and presence of cisplatin on the nanotubes was confirmed using energy-dispersive x-ray spectroscopy and Fourier transform infrared spectroscopy. STEM tomography was also used to provide additional insights concerning the nanotube conjugates. Finally, our observations provide a rationale for exploring the use of SWNT bioconjugates to selectively target and kill squamous cancer cells. Conclusion Z-contrast STEM imaging provides a means for direct visualization of heavy metal containing molecules (i.e., cisplatin) attached to surfaces of carbon SWNTs along with distribution and quantitation. PMID:19839812

  14. Biological object recognition in μ-radiography images

    NASA Astrophysics Data System (ADS)

    Prochazka, A.; Dammer, J.; Weyda, F.; Sopko, V.; Benes, J.; Zeman, J.; Jandejsek, I.

    2015-03-01

    This study presents an applicability of real-time microradiography to biological objects, namely to horse chestnut leafminer, Cameraria ohridella (Insecta: Lepidoptera, Gracillariidae) and following image processing focusing on image segmentation and object recognition. The microradiography of insects (such as horse chestnut leafminer) provides a non-invasive imaging that leaves the organisms alive. The imaging requires a high spatial resolution (micrometer scale) radiographic system. Our radiographic system consists of a micro-focus X-ray tube and two types of detectors. The first is a charge integrating detector (Hamamatsu flat panel), the second is a pixel semiconductor detector (Medipix2 detector). The latter allows detection of single quantum photon of ionizing radiation. We obtained numerous horse chestnuts leafminer pupae in several microradiography images easy recognizable in automatic mode using the image processing methods. We implemented an algorithm that is able to count a number of dead and alive pupae in images. The algorithm was based on two methods: 1) noise reduction using mathematical morphology filters, 2) Canny edge detection. The accuracy of the algorithm is higher for the Medipix2 (average recall for detection of alive pupae =0.99, average recall for detection of dead pupae =0.83), than for the flat panel (average recall for detection of alive pupae =0.99, average recall for detection of dead pupae =0.77). Therefore, we conclude that Medipix2 has lower noise and better displays contours (edges) of biological objects. Our method allows automatic selection and calculation of dead and alive chestnut leafminer pupae. It leads to faster monitoring of the population of one of the world's important insect pest.

  15. Fuzzy connected object definition in images with respect to co-objects

    NASA Astrophysics Data System (ADS)

    Udupa, Jayaram K.; Saha, Punam K.; Lotufo, Roberto A.

    1999-05-01

    Tangible solutions to practical image segmentation are vital to ensure progress in many applications of medical imaging. Toward this goal, we previously proposed a theory and algorithms for fuzzy connected object definition in n- dimensional images. Their effectiveness has been demonstrated in several applications including multiple sclerosis lesion detection/delineation, MR Angiography, and craniofacial imaging. The purpose of this work is to extend the earlier theory and algorithms to fuzzy connected object definition that considers all relevant objects in the image simultaneously. In the previous theory, delineation of the final object from the fuzzy connectivity scene required the selection of a threshold that specifies the weakest `hanging-togetherness' of image elements relative to each other in the object. Selection of such a threshold was not trivial and has been an active research area. In the proposed method of relative fuzzy connectivity, instead of defining an object on its own based on the strength of connectedness, all co-objects of importance that are present in the image are also considered and the objects are let to compete among themselves in having image elements as their members. In this competition, every pair of elements in the image will have a strength of connectedness in each object. The object in which this strength is highest will claim membership of the elements. This approach to fuzzy object definition using a relative strength of connectedness eliminates the need for a threshold of strength of connectedness that was part of the previous definition. It seems to be more natural since it relies on the fact that an object gets defined in an image by the presence of other objects that coexist in the image. All specified objects are defined simultaneously in this approach. The concept of iterative relative fuzzy connectivity has also been introduced. Robustness of relative fuzzy objects with respect to selection of reference image elements

  16. Fast single image dehazing based on image fusion

    NASA Astrophysics Data System (ADS)

    Liu, Haibo; Yang, Jie; Wu, Zhengping; Zhang, Qingnian

    2015-01-01

    Images captured in foggy weather conditions often fade the colors and reduce the contrast of the observed objects. An efficient image fusion method is proposed to remove haze from a single input image. First, the initial medium transmission is estimated based on the dark channel prior. Second, the method adopts an assumption that the degradation level affected by haze of each region is the same, which is similar to the Retinex theory, and uses a simple Gaussian filter to get the coarse medium transmission. Then, pixel-level fusion is achieved between the initial medium transmission and coarse medium transmission. The proposed method can recover a high-quality haze-free image based on the physical model, and the complexity of the proposed method is only a linear function of the number of input image pixels. Experimental results demonstrate that the proposed method can allow a very fast implementation and achieve better restoration for visibility and color fidelity compared to some state-of-the-art methods.

  17. Terrestrial scanning or digital images in inventory of monumental objects? - case study

    NASA Astrophysics Data System (ADS)

    Markiewicz, J. S.; Zawieska, D.

    2014-06-01

    Cultural heritage is the evidence of the past; monumental objects create the important part of the cultural heritage. Selection of a method to be applied depends on many factors, which include: the objectives of inventory, the object's volume, sumptuousness of architectural design, accessibility to the object, required terms and accuracy of works. The paper presents research and experimental works, which have been performed in the course of development of architectural documentation of elements of the external facades and interiors of the Wilanów Palace Museum in Warszawa. Point clouds, acquired from terrestrial laser scanning (Z+F 5003h) and digital images taken with Nikon D3X and Hasselblad H4D cameras were used. Advantages and disadvantages of utilisation of these technologies of measurements have been analysed with consideration of the influence of the structure and reflectance of investigated monumental surfaces on the quality of generation of photogrammetric products. The geometric quality of surfaces obtained from terrestrial laser scanning data and from point clouds resulting from digital images, have been compared.

  18. Mission planning optimization of video satellite for ground multi-object staring imaging

    NASA Astrophysics Data System (ADS)

    Cui, Kaikai; Xiang, Junhua; Zhang, Yulin

    2018-03-01

    This study investigates the emergency scheduling problem of ground multi-object staring imaging for a single video satellite. In the proposed mission scenario, the ground objects require a specified duration of staring imaging by the video satellite. The planning horizon is not long, i.e., it is usually shorter than one orbit period. A binary decision variable and the imaging order are used as the design variables, and the total observation revenue combined with the influence of the total attitude maneuvering time is regarded as the optimization objective. Based on the constraints of the observation time windows, satellite attitude adjustment time, and satellite maneuverability, a constraint satisfaction mission planning model is established for ground object staring imaging by a single video satellite. Further, a modified ant colony optimization algorithm with tabu lists (Tabu-ACO) is designed to solve this problem. The proposed algorithm can fully exploit the intelligence and local search ability of ACO. Based on full consideration of the mission characteristics, the design of the tabu lists can reduce the search range of ACO and improve the algorithm efficiency significantly. The simulation results show that the proposed algorithm outperforms the conventional algorithm in terms of optimization performance, and it can obtain satisfactory scheduling results for the mission planning problem.

  19. Images of Axial Objects

    ERIC Educational Resources Information Center

    Rabal, Hector; Cap, Nelly; Trivi, Marcelo

    2011-01-01

    Imaging of three-dimensional objects by lenses and mirrors is sometimes poorly indicated in textbooks and can be incorrectly drawn. We stress a need to clarify the concept of longitudinal magnification, with simulated images illustrating distortions introduced along the optical axis. We consider all possible positions of the object for both a…

  20. Recreation of three-dimensional objects in a real-time simulated environment by means of a panoramic single lens stereoscopic image-capturing device

    NASA Astrophysics Data System (ADS)

    Wong, Erwin

    2000-03-01

    Traditional methods of linear based imaging limits the viewer to a single fixed-point perspective. By means of a single lens multiple perspective mirror system, a 360-degree representation of the area around the camera is reconstructed. This reconstruction is used overcome the limitations of a traditional camera by providing the viewer with many different perspectives. By constructing the mirror into a hemispherical surface with multiple focal lengths at various diameters on the mirror, and by placing a parabolic mirror overhead, a stereoscopic image can be extracted from the image captured by a high-resolution camera placed beneath the mirror. Image extraction and correction is made by computer processing of the image obtained by camera; the image present up to five distinguishable different viewpoints that a computer can extrapolate pseudo- perspective data from. Geometric and depth for field can be extrapolated via comparison and isolation of objects within a virtual scene post processed by the computer. Combining data with scene rendering software provides the viewer with the ability to choose a desired viewing position, multiple dynamic perspectives, and virtually constructed perspectives based on minimal existing data. An examination into the workings of the mirror relay system is provided, including possible image extrapolation and correctional methods. Generation of data and virtual interpolated and constructed data is also mentioned.

  1. Z-depth integration: a new technique for manipulating z-depth properties in composited scenes

    NASA Astrophysics Data System (ADS)

    Steckel, Kayla; Whittinghill, David

    2014-02-01

    This paper presents a new technique in the production pipeline of asset creation for virtual environments called Z-Depth Integration (ZeDI). ZeDI is intended to reduce the time required to place elements at the appropriate z-depth within a scene. Though ZeDI is intended for use primarily in two-dimensional scene composition, depth-dependent "flat" animated objects are often critical elements of augmented and virtual reality applications (AR/VR). ZeDI is derived from "deep image compositing", a capacity implemented within the OpenEXR file format. In order to trick the human eye into perceiving overlapping scene elements as being in front of or behind one another, the developer must manually manipulate which pixels of an element are visible in relation to other objects embedded within the environment's image sequence. ZeDI improves on this process by providing a means for interacting with procedurally extracted z-depth data from a virtual environment scene. By streamlining the process of defining objects' depth characteristics, it is expected that the time and energy required for developers to create compelling AR/VR scenes will be reduced. In the proof of concept presented in this manuscript, ZeDI is implemented for pre-rendered virtual scene construction via an AfterEffects software plug-in.

  2. Large object investigation by digital holography with effective spectrum multiplexing under single-exposure approach

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Liu, Ning, E-mail: coolboy006@sohu.com; Zhang, Yingying; Xie, Jun

    2014-10-13

    We present a method to investigate large object by digital holography with effective spectrum multiplexing under single-exposure approach. This method splits the original reference beam and redirects one of its branches as a second object beam. Through the modified Mach-Zehnder interferometer, the two object beams can illuminate different parts of the large object and create a spectrum multiplexed hologram onto the focal plane array of the charge-coupled device/complementary metal oxide semiconductor camera. After correct spectrum extraction and image reconstruction, the large object can be fully observed within only one single snap-shot. The flexibility and great performance make our method amore » very attractive and promising technique for large object investigation under common 632.8 nm illumination.« less

  3. Object tracking using plenoptic image sequences

    NASA Astrophysics Data System (ADS)

    Kim, Jae Woo; Bae, Seong-Joon; Park, Seongjin; Kim, Do Hyung

    2017-05-01

    Object tracking is a very important problem in computer vision research. Among the difficulties of object tracking, partial occlusion problem is one of the most serious and challenging problems. To address the problem, we proposed novel approaches to object tracking on plenoptic image sequences. Our approaches take advantage of the refocusing capability that plenoptic images provide. Our approaches input the sequences of focal stacks constructed from plenoptic image sequences. The proposed image selection algorithms select the sequence of optimal images that can maximize the tracking accuracy from the sequence of focal stacks. Focus measure approach and confidence measure approach were proposed for image selection and both of the approaches were validated by the experiments using thirteen plenoptic image sequences that include heavily occluded target objects. The experimental results showed that the proposed approaches were satisfactory comparing to the conventional 2D object tracking algorithms.

  4. Objective Assessment of Image Quality VI: Imaging in Radiation Therapy

    PubMed Central

    Barrett, Harrison H.; Kupinski, Matthew A.; Müeller, Stefan; Halpern, Howard J.; Morris, John C.; Dwyer, Roisin

    2015-01-01

    Earlier work on Objective Assessment of Image Quality (OAIQ) focused largely on estimation or classification tasks in which the desired outcome of imaging is accurate diagnosis. This paper develops a general framework for assessing imaging quality on the basis of therapeutic outcomes rather than diagnostic performance. By analogy to Receiver Operating Characteristic (ROC) curves and their variants as used in diagnostic OAIQ, the method proposed here utilizes the Therapy Operating Characteristic or TOC curves, which are plots of the probability of tumor control vs. the probability of normal-tissue complications as the overall dose level of a radiotherapy treatment is varied. The proposed figure of merit is the area under the TOC curve, denoted AUTOC. This paper reviews an earlier exposition of the theory of TOC and AUTOC, which was specific to the assessment of image-segmentation algorithms, and extends it to other applications of imaging in external-beam radiation treatment as well as in treatment with internal radioactive sources. For each application, a methodology for computing the TOC is presented. A key difference between ROC and TOC is that the latter can be defined for a single patient rather than a population of patients. PMID:24200954

  5. Application of shift-and-add algorithms for imaging objects within biological media

    NASA Astrophysics Data System (ADS)

    Aizert, Avishai; Moshe, Tomer; Abookasis, David

    2017-01-01

    The Shift-and-Add (SAA) technique is a simple mathematical operation developed to reconstruct, at high spatial resolution, atmospherically degraded solar images obtained from stellar speckle interferometry systems. This method shifts and assembles individual degraded short-exposure images into a single average image with significantly improved contrast and detail. Since the inhomogeneous refractive indices of biological tissue causes light scattering similar to that induced by optical turbulence in the atmospheric layers, we assume that SAA methods can be successfully implemented to reconstruct the image of an object within a scattering biological medium. To test this hypothesis, five SAA algorithms were evaluated for reconstructing images acquired from multiple viewpoints. After successfully retrieving the hidden object's shape, quantitative image quality metrics were derived, enabling comparison of imaging error across a spectrum of layer thicknesses, demonstrating the relative efficacy of each SAA algorithm for biological imaging.

  6. Comparison of using single- or multi-polarimetric TerraSAR-X images for segmentation and classification of man-made maritime objects

    NASA Astrophysics Data System (ADS)

    Teutsch, Michael; Saur, Günter

    2011-11-01

    Spaceborne SAR imagery offers high capability for wide-ranging maritime surveillance especially in situations, where AIS (Automatic Identification System) data is not available. Therefore, maritime objects have to be detected and optional information such as size, orientation, or object/ship class is desired. In recent research work, we proposed a SAR processing chain consisting of pre-processing, detection, segmentation, and classification for single-polarimetric (HH) TerraSAR-X StripMap images to finally assign detection hypotheses to class "clutter", "non-ship", "unstructured ship", or "ship structure 1" (bulk carrier appearance) respectively "ship structure 2" (oil tanker appearance). In this work, we extend the existing processing chain and are now able to handle full-polarimetric (HH, HV, VH, VV) TerraSAR-X data. With the possibility of better noise suppression using the different polarizations, we slightly improve both the segmentation and the classification process. In several experiments we demonstrate the potential benefit for segmentation and classification. Precision of size and orientation estimation as well as correct classification rates are calculated individually for single- and quad-polarization and compared to each other.

  7. Multicolor, time-gated, soft x-ray pinhole imaging of wire array and gas puff Z pinches on the Z and Saturn pulsed power generators.

    PubMed

    Jones, B; Coverdale, C A; Nielsen, D S; Jones, M C; Deeney, C; Serrano, J D; Nielsen-Weber, L B; Meyer, C J; Apruzese, J P; Clark, R W; Coleman, P L

    2008-10-01

    A multicolor, time-gated, soft x-ray pinhole imaging instrument is fielded as part of the core diagnostic set on the 25 MA Z machine [M. E. Savage et al., in Proceedings of the Pulsed Power Plasma Sciences Conference (IEEE, New York, 2007), p. 979] for studying intense wire array and gas puff Z-pinch soft x-ray sources. Pinhole images are reflected from a planar multilayer mirror, passing 277 eV photons with <10 eV bandwidth. An adjacent pinhole camera uses filtration alone to view 1-10 keV photons simultaneously. Overlaying these data provides composite images that contain both spectral as well as spatial information, allowing for the study of radiation production in dense Z-pinch plasmas. Cu wire arrays at 20 MA on Z show the implosion of a colder cloud of material onto a hot dense core where K-shell photons are excited. A 528 eV imaging configuration has been developed on the 8 MA Saturn generator [R. B. Spielman et al., and A. I. P. Conf, Proc. 195, 3 (1989)] for imaging a bright Li-like Ar L-shell line. Ar gas puff Z pinches show an intense K-shell emission from a zippering stagnation front with L-shell emission dominating as the plasma cools.

  8. Image Tiling for Profiling Large Objects

    NASA Technical Reports Server (NTRS)

    Venkataraman, Ajit; Schock, Harold; Mercer, Carolyn R.

    1992-01-01

    Three dimensional surface measurements of large objects arc required in a variety of industrial processes. The nature of these measurements is changing as optical instruments arc beginning to replace conventional contact probes scanned over the objects. A common characteristic of the optical surface profilers is the trade off between measurement accuracy and field of view. In order to measure a large object with high accuracy, multiple views arc required. An accurate transformation between the different views is needed to bring about their registration. In this paper, we demonstrate how the transformation parameters can be obtained precisely by choosing control points which lie in the overlapping regions of the images. A good starting point for the transformation parameters is obtained by having a knowledge of the scanner position. The selection of the control points arc independent of the object geometry. By successively recording multiple views and obtaining transformation with respect to a single coordinate system, a complete physical model of an object can be obtained. Since all data arc in the same coordinate system, it can thus be used for building automatic models for free form surfaces.

  9. Object extraction method for image synthesis

    NASA Astrophysics Data System (ADS)

    Inoue, Seiki

    1991-11-01

    The extraction of component objects from images is fundamentally important for image synthesis. In TV program production, one useful method is the Video-Matte technique for specifying the necessary boundary of an object. This, however, involves some manually intricate and tedious processes. A new method proposed in this paper can reduce the needed level of operator skill and simplify object extraction. The object is automatically extracted by just a simple drawing of a thick boundary line. The basic principle involves a thinning of the thick boundary line binary image using the edge intensity of the original image. This method has many practical advantages, including the simplicity of specifying an object, the high accuracy of thinned-out boundary line, its ease of application to moving images, and the lack of any need for adjustment.

  10. Method for imaging a concealed object

    DOEpatents

    Davidson, James R [Idaho Falls, ID; Partin, Judy K [Idaho Falls, ID; Sawyers, Robert J [Idaho Falls, ID

    2007-07-03

    A method for imaging a concealed object is described and which includes a step of providing a heat radiating body, and wherein an object to be detected is concealed on the heat radiating body; imaging the heat radiating body to provide a visibly discernible infrared image of the heat radiating body; and determining if the visibly discernible infrared image of the heat radiating body is masked by the presence of the concealed object.

  11. The optical lens coupled X-ray in-line phase contrast imaging system for the characterization of low Z materials

    NASA Astrophysics Data System (ADS)

    Wang, Kai; Lin, Wei; Dai, Fei; Li, Jun; Qi, Xiaobo; Lei, Haile; Liu, Yuanqiong

    2018-05-01

    Due to the high spatial resolution and contrast, the optical lens coupled X-ray in-line phase contrast imaging system with the secondary optical magnification is more suitable for the characterization of the low Z materials. The influence of the source to object distance and the object to scintillator distance on the image resolution and contrast is studied experimentally. A phase correlation algorithm is used for the image mosaic of a serial of X-ray phase contrast images acquired with high resolution, the resulting resolution is less than 1.0 μm, and the whole field of view is larger than 1.4 mm. Finally, the geometric morphology and the inner structure of various weakly absorbing samples and the evaporation of water in the plastic micro-shell are in situ characterized by the optical lens coupled X-ray in-line phase contrast imaging system.

  12. Distinct cognitive mechanisms involved in the processing of single objects and object ensembles

    PubMed Central

    Cant, Jonathan S.; Sun, Sol Z.; Xu, Yaoda

    2015-01-01

    Behavioral research has demonstrated that the shape and texture of single objects can be processed independently. Similarly, neuroimaging results have shown that an object's shape and texture are processed in distinct brain regions with shape in the lateral occipital area and texture in parahippocampal cortex. Meanwhile, objects are not always seen in isolation and are often grouped together as an ensemble. We recently showed that the processing of ensembles also involves parahippocampal cortex and that the shape and texture of ensemble elements are processed together within this region. These neural data suggest that the independence seen between shape and texture in single-object perception would not be observed in object-ensemble perception. Here we tested this prediction by examining whether observers could attend to the shape of ensemble elements while ignoring changes in an unattended texture feature and vice versa. Across six behavioral experiments, we replicated previous findings of independence between shape and texture in single-object perception. In contrast, we observed that changes in an unattended ensemble feature negatively impacted the processing of an attended ensemble feature only when ensemble features were attended globally. When they were attended locally, thereby making ensemble processing similar to single-object processing, interference was abolished. Overall, these findings confirm previous neuroimaging results and suggest that distinct cognitive mechanisms may be involved in single-object and object-ensemble perception. Additionally, they show that the scope of visual attention plays a critical role in determining which type of object processing (ensemble or single object) is engaged by the visual system. PMID:26360156

  13. Shadow-free single-pixel imaging

    NASA Astrophysics Data System (ADS)

    Li, Shunhua; Zhang, Zibang; Ma, Xiao; Zhong, Jingang

    2017-11-01

    Single-pixel imaging is an innovative imaging scheme and receives increasing attention in recent years, for it is applicable for imaging at non-visible wavelengths and imaging under weak light conditions. However, as in conventional imaging, shadows would likely occur in single-pixel imaging and sometimes bring negative effects in practical uses. In this paper, the principle of shadows occurrence in single-pixel imaging is analyzed, following which a technique for shadows removal is proposed. In the proposed technique, several single-pixel detectors are used to detect the backscattered light at different locations so that the shadows in the reconstructed images corresponding to each detector shadows are complementary. Shadow-free reconstruction can be derived by fusing the shadow-complementary images using maximum selection rule. To deal with the problem of intensity mismatch in image fusion, we put forward a simple calibration. As experimentally demonstrated, the technique is able to reconstruct monochromatic and full-color shadow-free images.

  14. Single shot imaging through turbid medium and around corner using coherent light

    NASA Astrophysics Data System (ADS)

    Li, Guowei; Li, Dayan; Situ, Guohai

    2018-01-01

    Optical imaging through turbid media and around corner is a difficult challenge. Even a very thin layer of a turbid media, which randomly scatters the probe light, can appear opaque and hide any objects behind it. Despite many recent advances, no current method can image the object behind turbid media with single record using coherent laser illumination. Here we report a method that allows non-invasive single-shot optical imaging through turbid media and around corner via speckle correlation. Instead of being as an obstacle in forming diffractionlimited images, speckle actually can be a carrier that encodes sufficient information to imaging through visually opaque layers. Optical imaging through turbid media and around corner is experimentally demonstrated using traditional imaging system with the aid of iterative phase retrieval algorithm. Our method require neither scan of illumination nor two-arm interferometry or long-time exposure in acquisition, which has new implications in optical sensing through common obscurants such as fog, smoke and haze.

  15. Linear and ultrafast nonlinear plasmonics of single nano-objects

    NASA Astrophysics Data System (ADS)

    Crut, Aurélien; Maioli, Paolo; Vallée, Fabrice; Del Fatti, Natalia

    2017-03-01

    Single-particle optical investigations have greatly improved our understanding of the fundamental properties of nano-objects, avoiding the spurious inhomogeneous effects that affect ensemble experiments. Correlation with high-resolution imaging techniques providing morphological information (e.g. electron microscopy) allows a quantitative interpretation of the optical measurements by means of analytical models and numerical simulations. In this topical review, we first briefly recall the principles underlying some of the most commonly used single-particle optical techniques: near-field, dark-field, spatial modulation and photothermal microscopies/spectroscopies. We then focus on the quantitative investigation of the surface plasmon resonance (SPR) of metallic nano-objects using linear and ultrafast optical techniques. While measured SPR positions and spectral areas are found in good agreement with predictions based on Maxwell’s equations, SPR widths are strongly influenced by quantum confinement (or, from a classical standpoint, surface-induced electron scattering) and, for small nano-objects, cannot be reproduced using the dielectric functions of bulk materials. Linear measurements on single nano-objects (silver nanospheres and gold nanorods) allow a quantification of the size and geometry dependences of these effects in confined metals. Addressing the ultrafast response of an individual nano-object is also a powerful tool to elucidate the physical mechanisms at the origin of their optical nonlinearities, and their electronic, vibrational and thermal relaxation processes. Experimental investigations of the dynamical response of gold nanorods are shown to be quantitatively modeled in terms of modifications of the metal dielectric function enhanced by plasmonic effects. Ultrafast spectroscopy can also be exploited to unveil hidden physical properties of more complex nanosystems. In this context, two-color femtosecond pump-probe experiments performed on individual

  16. Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution

    DOE PAGES

    Meddens, Marjolein B. M.; Liu, Sheng; Finnegan, Patrick S.; ...

    2016-01-01

    Here, we have developed a method for performing light-sheet microscopy with a single high numerical aperture lens by integrating reflective side walls into a microfluidic chip. These 45° side walls generate light-sheet illumination by reflecting a vertical light-sheet into the focal plane of the objective. Light-sheet illumination of cells loaded in the channels increases image quality in diffraction limited imaging via reduction of out-of-focus background light. Single molecule super-resolution is also improved by the decreased background resulting in better localization precision and decreased photo-bleaching, leading to more accepted localizations overall and higher quality images. Moreover, 2D and 3D single moleculemore » super-resolution data can be acquired faster by taking advantage of the increased illumination intensities as compared to wide field, in the focused light-sheet.« less

  17. Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution

    PubMed Central

    Meddens, Marjolein B. M.; Liu, Sheng; Finnegan, Patrick S.; Edwards, Thayne L.; James, Conrad D.; Lidke, Keith A.

    2016-01-01

    We have developed a method for performing light-sheet microscopy with a single high numerical aperture lens by integrating reflective side walls into a microfluidic chip. These 45° side walls generate light-sheet illumination by reflecting a vertical light-sheet into the focal plane of the objective. Light-sheet illumination of cells loaded in the channels increases image quality in diffraction limited imaging via reduction of out-of-focus background light. Single molecule super-resolution is also improved by the decreased background resulting in better localization precision and decreased photo-bleaching, leading to more accepted localizations overall and higher quality images. Moreover, 2D and 3D single molecule super-resolution data can be acquired faster by taking advantage of the increased illumination intensities as compared to wide field, in the focused light-sheet. PMID:27375939

  18. Buried object detection in GPR images

    DOEpatents

    Paglieroni, David W; Chambers, David H; Bond, Steven W; Beer, W. Reginald

    2014-04-29

    A method and system for detecting the presence of subsurface objects within a medium is provided. In some embodiments, the imaging and detection system operates in a multistatic mode to collect radar return signals generated by an array of transceiver antenna pairs that is positioned across the surface and that travels down the surface. The imaging and detection system pre-processes the return signal to suppress certain undesirable effects. The imaging and detection system then generates synthetic aperture radar images from real aperture radar images generated from the pre-processed return signal. The imaging and detection system then post-processes the synthetic aperture radar images to improve detection of subsurface objects. The imaging and detection system identifies peaks in the energy levels of the post-processed image frame, which indicates the presence of a subsurface object.

  19. System for forming a quadrified image comprising angularly related fields of view of a three dimensional object

    NASA Technical Reports Server (NTRS)

    Chamberlain, F. R. (Inventor)

    1980-01-01

    A system for generating, within a single frame of photographic film, a quadrified image including images of angularly (including orthogonally) related fields of view of a near field three dimensional object is described. It is characterized by three subsystems each of which includes a plurality of reflective surfaces for imaging a different field of view of the object at a different quadrant of the quadrified image. All of the subsystems have identical path lengths to the object photographed.

  20. Host composition dependent tunable multicolor emission in the single-phase Ba2(Ln(1-z)Tb(z))(BO3)2Cl:Eu phosphors.

    PubMed

    Xia, Zhiguo; Zhuang, Jiaqing; Meijerink, Andries; Jing, Xiping

    2013-05-14

    A new strategy based on the host composition design has been adopted to obtain efficient color-tunable emission from Ba2Ln(0.97-z)Tb(z)(BO3)2Cl:0.03Eu (Ln = Y, Gd and Lu, z = 0-0.97) phosphors. This study reveals that the single-phase Ba2Ln(1-z)Tb(z)(BO3)2Cl compounds can be applied to use allowed Eu(2+) absorption transitions to sensitize Eu(3+) emission via the energy transfer Eu(2+) → (Tb(3+))n → Eu(3+). The powder X-ray diffraction (XRD) and Rietveld refinement analysis shows single-phase Ba2Ln(1-z)Tb(z)(BO3)2Cl. As-prepared Ba2Ln(0.97-z)Tb(z)(BO3)2Cl:0.03Eu phosphors show intense green, yellow, orange and red emission under 377 nm near ultraviolet (n-UV) excitation due to a variation in the relative intensities of the Eu(2+), Tb(3+) and Eu(3+) emission depending on the Tb content (z) in the host composition, allowing color tuning. The variation in emission color is explained by energy transfer and has been investigated by photoluminescence and lifetime measurements and is further characterized by the Commission Internationale de l'éclairage (CIE) chromaticity indexes. The quantum efficiencies of the phosphors are high, up to 74%, and show good thermal stabilities up to 150 °C. This investigation demonstrates the possibility to sensitize Eu(3+) line emission by Eu(2+)via energy migration over Tb(3+) resulting in efficient color tunable phosphors which are promising for use in solid-state white light-emitting diodes (w-LEDs).

  1. Orientation estimation of anatomical structures in medical images for object recognition

    NASA Astrophysics Data System (ADS)

    Bağci, Ulaş; Udupa, Jayaram K.; Chen, Xinjian

    2011-03-01

    Recognition of anatomical structures is an important step in model based medical image segmentation. It provides pose estimation of objects and information about "where" roughly the objects are in the image and distinguishing them from other object-like entities. In,1 we presented a general method of model-based multi-object recognition to assist in segmentation (delineation) tasks. It exploits the pose relationship that can be encoded, via the concept of ball scale (b-scale), between the binary training objects and their associated grey images. The goal was to place the model, in a single shot, close to the right pose (position, orientation, and scale) in a given image so that the model boundaries fall in the close vicinity of object boundaries in the image. Unlike position and scale parameters, we observe that orientation parameters require more attention when estimating the pose of the model as even small differences in orientation parameters can lead to inappropriate recognition. Motivated from the non-Euclidean nature of the pose information, we propose in this paper the use of non-Euclidean metrics to estimate orientation of the anatomical structures for more accurate recognition and segmentation. We statistically analyze and evaluate the following metrics for orientation estimation: Euclidean, Log-Euclidean, Root-Euclidean, Procrustes Size-and-Shape, and mean Hermitian metrics. The results show that mean Hermitian and Cholesky decomposition metrics provide more accurate orientation estimates than other Euclidean and non-Euclidean metrics.

  2. An object-oriented framework for medical image registration, fusion, and visualization.

    PubMed

    Zhu, Yang-Ming; Cochoff, Steven M

    2006-06-01

    An object-oriented framework for image registration, fusion, and visualization was developed based on the classic model-view-controller paradigm. The framework employs many design patterns to facilitate legacy code reuse, manage software complexity, and enhance the maintainability and portability of the framework. Three sample applications built a-top of this framework are illustrated to show the effectiveness of this framework: the first one is for volume image grouping and re-sampling, the second one is for 2D registration and fusion, and the last one is for visualization of single images as well as registered volume images.

  3. High energy X-ray pinhole imaging at the Z facility

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McPherson, L. Armon; Ampleford, David J.; Coverdale, Christine A.

    A new high photon energy (hv > 15 keV) time-integrated pinhole camera (TIPC) has become available at the Z facility for diagnostic applications. This camera employs five pinholes in a linear array for recording five images at once onto an image plate detector. Each pinhole may be independently filtered to yield five different spectral responses. The pinhole array is fabricated from a 1-cm thick tungsten block and is available with either straight pinholes or conical pinholes. Each pinhole within the array block is 250 μm in diameter. The five pinholes are splayed with respect to each other such that theymore » point to the same location in space, and hence present the same view of the target load at the Z facility. The fielding distance is 66 cm and the nominal image magnification is 0.374. Initial experimental results are shown to illustrate the performance of the camera.« less

  4. High energy X-ray pinhole imaging at the Z facility

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McPherson, L. Armon; Ampleford, David J., E-mail: damplef@sandia.gov; Coverdale, Christine A.

    A new high photon energy (hν > 15 keV) time-integrated pinhole camera (TIPC) has been developed as a diagnostic instrument at the Z facility. This camera employs five pinholes in a linear array for recording five images at once onto an image plate detector. Each pinhole may be independently filtered to yield five different spectral responses. The pinhole array is fabricated from a 1-cm thick tungsten block and is available with either straight pinholes or conical pinholes. Each pinhole within the array block is 250 μm in diameter. The five pinholes are splayed with respect to each other such thatmore » they point to the same location in space, and hence present the same view of the radiation source at the Z facility. The fielding distance from the radiation source is 66 cm and the nominal image magnification is 0.374. Initial experimental results from TIPC are shown to illustrate the performance of the camera.« less

  5. High energy X-ray pinhole imaging at the Z facility

    DOE PAGES

    McPherson, L. Armon; Ampleford, David J.; Coverdale, Christine A.; ...

    2016-06-06

    A new high photon energy (hv > 15 keV) time-integrated pinhole camera (TIPC) has become available at the Z facility for diagnostic applications. This camera employs five pinholes in a linear array for recording five images at once onto an image plate detector. Each pinhole may be independently filtered to yield five different spectral responses. The pinhole array is fabricated from a 1-cm thick tungsten block and is available with either straight pinholes or conical pinholes. Each pinhole within the array block is 250 μm in diameter. The five pinholes are splayed with respect to each other such that theymore » point to the same location in space, and hence present the same view of the target load at the Z facility. The fielding distance is 66 cm and the nominal image magnification is 0.374. Initial experimental results are shown to illustrate the performance of the camera.« less

  6. High-z objects and cold dark matter cosmogonies - Constraints on the primordial power spectrum on small scales

    NASA Technical Reports Server (NTRS)

    Kashlinsky, A.

    1993-01-01

    Modified cold dark matter (CDM) models were recently suggested to account for large-scale optical data, which fix the power spectrum on large scales, and the COBE results, which would then fix the bias parameter, b. We point out that all such models have deficit of small-scale power where density fluctuations are presently nonlinear, and should then lead to late epochs of collapse of scales M between 10 exp 9 - 10 exp 10 solar masses and (1-5) x 10 exp 14 solar masses. We compute the probabilities and comoving space densities of various scale objects at high redshifts according to the CDM models and compare these with observations of high-z QSOs, high-z galaxies and the protocluster-size object found recently by Uson et al. (1992) at z = 3.4. We show that the modified CDM models are inconsistent with the observational data on these objects. We thus suggest that in order to account for the high-z objects, as well as the large-scale and COBE data, one needs a power spectrum with more power on small scales than CDM models allow and an open universe.

  7. Ball-scale based hierarchical multi-object recognition in 3D medical images

    NASA Astrophysics Data System (ADS)

    Bağci, Ulas; Udupa, Jayaram K.; Chen, Xinjian

    2010-03-01

    This paper investigates, using prior shape models and the concept of ball scale (b-scale), ways of automatically recognizing objects in 3D images without performing elaborate searches or optimization. That is, the goal is to place the model in a single shot close to the right pose (position, orientation, and scale) in a given image so that the model boundaries fall in the close vicinity of object boundaries in the image. This is achieved via the following set of key ideas: (a) A semi-automatic way of constructing a multi-object shape model assembly. (b) A novel strategy of encoding, via b-scale, the pose relationship between objects in the training images and their intensity patterns captured in b-scale images. (c) A hierarchical mechanism of positioning the model, in a one-shot way, in a given image from a knowledge of the learnt pose relationship and the b-scale image of the given image to be segmented. The evaluation results on a set of 20 routine clinical abdominal female and male CT data sets indicate the following: (1) Incorporating a large number of objects improves the recognition accuracy dramatically. (2) The recognition algorithm can be thought as a hierarchical framework such that quick replacement of the model assembly is defined as coarse recognition and delineation itself is known as finest recognition. (3) Scale yields useful information about the relationship between the model assembly and any given image such that the recognition results in a placement of the model close to the actual pose without doing any elaborate searches or optimization. (4) Effective object recognition can make delineation most accurate.

  8. Frequency bandwidth extension by use of multiple Zeeman field offsets for electron spin-echo EPR oxygen imaging of large objects

    PubMed Central

    Seifi, Payam; Epel, Boris; Sundramoorthy, Subramanian V.; Mailer, Colin; Halpern, Howard J.

    2011-01-01

    Purpose: Electron spin-echo (ESE) oxygen imaging is a new and evolving electron paramagnetic resonance (EPR) imaging (EPRI) modality that is useful for physiological in vivo applications, such as EPR oxygen imaging (EPROI), with potential application to imaging of multicentimeter objects as large as human tumors. A present limitation on the size of the object to be imaged at a given resolution is the frequency bandwidth of the system, since the location is encoded as a frequency offset in ESE imaging. The authors’ aim in this study was to demonstrate the object size advantage of the multioffset bandwidth extension technique.Methods: The multiple-stepped Zeeman field offset (or simply multi-B) technique was used for imaging of an 8.5-cm-long phantom containing a narrow single line triaryl methyl compound (trityl) solution at the 250 MHz imaging frequency. The image is compared to a standard single-field ESE image of the same phantom.Results: For the phantom used in this study, transverse relaxation (T2e) electron spin-echo (ESE) images from multi-B acquisition are more uniform, contain less prominent artifacts, and have a better signal to noise ratio (SNR) compared to single-field T2e images.Conclusions: The multi-B method is suitable for imaging of samples whose physical size restricts the applicability of the conventional single-field ESE imaging technique. PMID:21815379

  9. The relevance of ultrasound imaging of suspicious axillary lymph nodes and fine-needle aspiration biopsy in the post ACOSOG Z11 era in early breast cancer

    PubMed Central

    Vijayaraghavan, Gopal R.; Vedantham, Srinivasan; Kataoka, Milliam; DeBenedectis, Carolynn; Quinlan, Robert

    2016-01-01

    Rationale and Objective Evaluation of nodal involvement in early-stage breast cancers (T1 or T2) changed following the Z11 trial; however, not all patients meet the Z11 inclusion criteria. Hence, the relevance of ultrasound imaging of the axilla and fine-needle aspiration biopsy (FNA) in early-stage breast cancers was investigated. Materials and Methods In this single-center, retrospective study, 758 subjects had pathology-verified breast cancer diagnosis over a 3-year period, of which 128 subjects with T1/T2 breast tumors had abnormal axillary lymph nodes on ultrasound, had FNA, and proceeded to axillary surgery. Ultrasound images were reviewed and analyzed using multivariable logistic regression to identify the features predictive of positive FNA. Accuracy of FNA was quantified as the area under the receiver operating characteristic curve with axillary surgery as reference standard. Results Of 128 subjects, 61 and 65 were positive on FNA and axillary surgery, respectively. Sensitivity, specificity, positive- and negative-predictive values of FNA were 52/65 (80%), 54/63 (85.7%), 52/61(85.2%) and 54/67 (80.5%), respectively. After adjusting for neoadjuvant chemotherapy between FNA and surgery, a positive FNA was associated with higher likelihood for positive axillary surgery (odds ratio: 22.7; 95% CI: 7.2–71.3, p<0.0001), and the accuracy of FNA was 0.801 (95% CI: 0.727–0.876). Among ultrasound imaging features, cortical thickness and abnormal hilum were predictive (p<0.017) of positive FNA with accuracy of 0.817 (95% CI: 0.741–0.893). Conclusion Ultrasound imaging and FNA can play an important role in the management of early breast cancers even in the post-Z11 era. Higher weightage can be accorded to cortical thickness and hilum during ultrasound evaluation. PMID:27916595

  10. Image reconstruction of dynamic infrared single-pixel imaging system

    NASA Astrophysics Data System (ADS)

    Tong, Qi; Jiang, Yilin; Wang, Haiyan; Guo, Limin

    2018-03-01

    Single-pixel imaging technique has recently received much attention. Most of the current single-pixel imaging is aimed at relatively static targets or the imaging system is fixed, which is limited by the number of measurements received through the single detector. In this paper, we proposed a novel dynamic compressive imaging method to solve the imaging problem, where exists imaging system motion behavior, for the infrared (IR) rosette scanning system. The relationship between adjacent target images and scene is analyzed under different system movement scenarios. These relationships are used to build dynamic compressive imaging models. Simulation results demonstrate that the proposed method can improve the reconstruction quality of IR image and enhance the contrast between the target and the background in the presence of system movement.

  11. Subaru High-z Exploration of Low-luminosity Quasars (SHELLQs). I. Discovery of 15 Quasars and Bright Galaxies at 5.7 < z < 6.9

    NASA Astrophysics Data System (ADS)

    Matsuoka, Yoshiki; Onoue, Masafusa; Kashikawa, Nobunari; Iwasawa, Kazushi; Strauss, Michael A.; Nagao, Tohru; Imanishi, Masatoshi; Niida, Mana; Toba, Yoshiki; Akiyama, Masayuki; Asami, Naoko; Bosch, James; Foucaud, Sébastien; Furusawa, Hisanori; Goto, Tomotsugu; Gunn, James E.; Harikane, Yuichi; Ikeda, Hiroyuki; Kawaguchi, Toshihiro; Kikuta, Satoshi; Komiyama, Yutaka; Lupton, Robert H.; Minezaki, Takeo; Miyazaki, Satoshi; Morokuma, Tomoki; Murayama, Hitoshi; Nishizawa, Atsushi J.; Ono, Yoshiaki; Ouchi, Masami; Price, Paul A.; Sameshima, Hiroaki; Silverman, John D.; Sugiyama, Naoshi; Tait, Philip J.; Takada, Masahiro; Takata, Tadafumi; Tanaka, Masayuki; Tang, Ji-Jia; Utsumi, Yousuke

    2016-09-01

    We report the discovery of 15 quasars and bright galaxies at 5.7 < z < 6.9. This is the initial result from the Subaru High-z Exploration of Low-Luminosity Quasars project, which exploits the exquisite multiband imaging data produced by the Subaru Hyper Suprime-Cam (HSC) Strategic Program survey. The candidate selection is performed by combining several photometric approaches including a Bayesian probabilistic algorithm to reject stars and dwarfs. The spectroscopic identification was carried out with the Gran Telescopio Canarias and the Subaru Telescope for the first 80 deg2 of the survey footprint. The success rate of our photometric selection is quite high, approaching 100% at the brighter magnitudes (z AB < 23.5 mag). Our selection also recovered all the known high-z quasars on the HSC images. Among the 15 discovered objects, six are likely quasars, while the other six with interstellar absorption lines and in some cases narrow emission lines are likely bright Lyman-break galaxies. The remaining three objects have weak continua and very strong and narrow Lyα lines, which may be excited by ultraviolet light from both young stars and quasars. These results indicate that we are starting to see the steep rise of the luminosity function of z ≥ 6 galaxies, compared with that of quasars, at magnitudes fainter than M 1450 ˜ -22 mag or z AB ˜ 24 mag. Follow-up studies of the discovered objects as well as further survey observations are ongoing.

  12. 3D localization of electrophysiology catheters from a single x-ray cone-beam projection

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Robert, Normand, E-mail: normand.robert@sri.utoronto.ca; Polack, George G.; Sethi, Benu

    2015-10-15

    Purpose: X-ray images allow the visualization of percutaneous devices such as catheters in real time but inherently lack depth information. The provision of 3D localization of these devices from cone beam x-ray projections would be advantageous for interventions such as electrophysiology (EP), whereby the operator needs to return a device to the same anatomical locations during the procedure. A method to achieve real-time 3D single view localization (SVL) of an object of known geometry from a single x-ray image is presented. SVL exploits the change in the magnification of an object as its distance from the x-ray source is varied.more » The x-ray projection of an object of interest is compared to a synthetic x-ray projection of a model of said object as its pose is varied. Methods: SVL was tested with a 3 mm spherical marker and an electrophysiology catheter. The effect of x-ray acquisition parameters on SVL was investigated. An independent reference localization method was developed to compare results when imaging a catheter translated via a computer controlled three-axes stage. SVL was also performed on clinical fluoroscopy image sequences. A commercial navigation system was used in some clinical image sequences for comparison. Results: SVL estimates exhibited little change as x-ray acquisition parameters were varied. The reproducibility of catheter position estimates in phantoms denoted by the standard deviations, (σ{sub x}, σ{sub y}, σ{sub z}) = (0.099 mm,  0.093 mm,  2.2 mm), where x and y are parallel to the detector plane and z is the distance from the x-ray source. Position estimates (x, y, z) exhibited a 4% systematic error (underestimation) when compared to the reference method. The authors demonstrated that EP catheters can be tracked in clinical fluoroscopic images. Conclusions: It has been shown that EP catheters can be localized in real time in phantoms and clinical images at fluoroscopic exposure rates. Further work is required to

  13. Diffusion-weighted imaging of the sellar region: a comparison study of BLADE and single-shot echo planar imaging sequences.

    PubMed

    Yiping, Lu; Hui, Liu; Kun, Zhou; Daoying, Geng; Bo, Yin

    2014-07-01

    The purpose of this study is to compare BLADE diffusion-weighted imaging (DWI) with single-shot echo planar imaging (EPI) DWI on the aspects of feasibility of imaging the sellar region and image quality. A total of 3 healthy volunteers and 52 patients with suspected lesions in the sellar region were included in this prospective intra-individual study. All exams were performed at 3.0T with a BLADE DWI sequence and a standard single-shot EP-DWI sequence. Phantom measurements were performed to measure the objective signal-to-noise ratio (SNR). Two radiologists rated the image quality according to the visualisation of the internal carotid arteries, optic chiasm, pituitary stalk, pituitary gland and lesion, and the overall image quality. One radiologist measured lesion sizes for detecting their relationship with the image score. The SNR in BLADE DWI sequence showed no significant difference from the single-shot EPI sequence (P>0.05). All of the assessed regions received higher scores in BLADE DWI images than single-shot EP-DWI. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

  14. [Impact to Z-score Mapping of Hyperacute Stroke Images by Computed Tomography in Adaptive Statistical Iterative Reconstruction].

    PubMed

    Watanabe, Shota; Sakaguchi, Kenta; Hosono, Makoto; Ishii, Kazunari; Murakami, Takamichi; Ichikawa, Katsuhiro

    The purpose of this study was to evaluate the effect of a hybrid-type iterative reconstruction method on Z-score mapping of hyperacute stroke in unenhanced computed tomography (CT) images. We used a hybrid-type iterative reconstruction [adaptive statistical iterative reconstruction (ASiR)] implemented in a CT system (Optima CT660 Pro advance, GE Healthcare). With 15 normal brain cases, we reconstructed CT images with a filtered back projection (FBP) and ASiR with a blending factor of 100% (ASiR100%). Two standardized normal brain data were created from normal databases of FBP images (FBP-NDB) and ASiR100% images (ASiR-NDB), and standard deviation (SD) values in basal ganglia were measured. The Z-score mapping was performed for 12 hyperacute stroke cases by using FBP-NDB and ASiR-NDB, and compared Z-score value on hyperacute stroke area and normal area between FBP-NDB and ASiR-NDB. By using ASiR-NDB, the SD value of standardized brain was decreased by 16%. The Z-score value of ASiR-NDB on hyperacute stroke area was significantly higher than FBP-NDB (p<0.05). Therefore, the use of images reconstructed with ASiR100% for Z-score mapping had potential to improve the accuracy of Z-score mapping.

  15. zWEDGI: Wounding and Entrapment Device for Imaging Live Zebrafish Larvae

    PubMed Central

    Huemer, Kayla; Squirrell, Jayne M.; Swader, Robert; LeBert, Danny C.; Huttenlocher, Anna; Eliceiri, Kevin W.

    2017-01-01

    Abstract Zebrafish, an established model organism in developmental biology, is also a valuable tool for imaging wound healing in space and time with cellular resolution. However, long-term imaging of wound healing poses technical challenges as wound healing occurs over multiple temporal scales. The traditional strategy of larval encapsulation in agarose successfully limits sample movement but impedes larval development and tissue regrowth and is therefore not amenable to long-term imaging of wound healing. To overcome this challenge, we engineered a functionally compartmentalized device, the zebrafish Wounding and Entrapment Device for Growth and Imaging (zWEDGI), to orient larvae for high-resolution microscopy, including confocal and second harmonic generation (SHG), while allowing unrestrained tail development and regrowth. In this device, larval viability was maintained and tail regrowth was improved over embedding in agarose. The quality of tail fiber SHG images collected from larvae in the device was similar to fixed samples but provided the benefit of time lapse data collection. Furthermore, we show that this device was amenable to long-term (>24 h) confocal microscopy of the caudal fin. Finally, the zWEDGI was designed and fabricated using readily available techniques so that it can be easily modified for diverse experimental imaging protocols. PMID:27676647

  16. Simultaneously Discovering and Localizing Common Objects in Wild Images.

    PubMed

    Wang, Zhenzhen; Yuan, Junsong

    2018-09-01

    Motivated by the recent success of supervised and weakly supervised common object discovery, in this paper, we move forward one step further to tackle common object discovery in a fully unsupervised way. Generally, object co-localization aims at simultaneously localizing objects of the same class across a group of images. Traditional object localization/detection usually trains specific object detectors which require bounding box annotations of object instances, or at least image-level labels to indicate the presence/absence of objects in an image. Given a collection of images without any annotations, our proposed fully unsupervised method is to simultaneously discover images that contain common objects and also localize common objects in corresponding images. Without requiring to know the total number of common objects, we formulate this unsupervised object discovery as a sub-graph mining problem from a weighted graph of object proposals, where nodes correspond to object proposals, and edges represent the similarities between neighbouring proposals. The positive images and common objects are jointly discovered by finding sub-graphs of strongly connected nodes, with each sub-graph capturing one object pattern. The optimization problem can be efficiently solved by our proposed maximal-flow-based algorithm. Instead of assuming that each image contains only one common object, our proposed solution can better address wild images where each image may contain multiple common objects or even no common object. Moreover, our proposed method can be easily tailored to the task of image retrieval in which the nodes correspond to the similarity between query and reference images. Extensive experiments on PASCAL VOC 2007 and Object Discovery data sets demonstrate that even without any supervision, our approach can discover/localize common objects of various classes in the presence of scale, view point, appearance variation, and partial occlusions. We also conduct broad

  17. Single-exposure quantitative phase imaging in color-coded LED microscopy.

    PubMed

    Lee, Wonchan; Jung, Daeseong; Ryu, Suho; Joo, Chulmin

    2017-04-03

    We demonstrate single-shot quantitative phase imaging (QPI) in a platform of color-coded LED microscopy (cLEDscope). The light source in a conventional microscope is replaced by a circular LED pattern that is trisected into subregions with equal area, assigned to red, green, and blue colors. Image acquisition with a color image sensor and subsequent computation based on weak object transfer functions allow for the QPI of a transparent specimen. We also provide a correction method for color-leakage, which may be encountered in implementing our method with consumer-grade LEDs and image sensors. Most commercially available LEDs and image sensors do not provide spectrally isolated emissions and pixel responses, generating significant error in phase estimation in our method. We describe the correction scheme for this color-leakage issue, and demonstrate improved phase measurement accuracy. The computational model and single-exposure QPI capability of our method are presented by showing images of calibrated phase samples and cellular specimens.

  18. System for objective assessment of image differences in digital cinema

    NASA Astrophysics Data System (ADS)

    Fliegel, Karel; Krasula, Lukáš; Páta, Petr; Myslík, Jiří; Pecák, Josef; Jícha, Marek

    2014-09-01

    There is high demand for quick digitization and subsequent image restoration of archived film records. Digitization is very urgent in many cases because various invaluable pieces of cultural heritage are stored on aging media. Only selected records can be reconstructed perfectly using painstaking manual or semi-automatic procedures. This paper aims to answer the question what are the quality requirements on the restoration process in order to obtain acceptably close visual perception of the digitally restored film in comparison to the original analog film copy. This knowledge is very important to preserve the original artistic intention of the movie producers. Subjective experiment with artificially distorted images has been conducted in order to answer the question what is the visual impact of common image distortions in digital cinema. Typical color and contrast distortions were introduced and test images were presented to viewers using digital projector. Based on the outcome of this subjective evaluation a system for objective assessment of image distortions has been developed and its performance tested. The system utilizes calibrated digital single-lens reflex camera and subsequent analysis of suitable features of images captured from the projection screen. The evaluation of captured image data has been optimized in order to obtain predicted differences between the reference and distorted images while achieving high correlation with the results of subjective assessment. The system can be used to objectively determine the difference between analog film and digital cinema images on the projection screen.

  19. Single-Grating Talbot Imaging for Wavefront Sensing and X-Ray Metrology

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Grizolli, Walan; Shi, Xianbo; Kolodziej, Tomasz

    2017-01-01

    Single-grating Talbot imaging relies on high-spatial-resolution detectors to perform accurate measurements of X-ray beam wavefronts. The wavefront can be retrieved with a single image, and a typical measurement and data analysis can be performed in few seconds. These qualities make it an ideal tool for synchrotron beamline diagnostics and in-situ metrology. The wavefront measurement can be used both to obtain a phase contrast image of an object and to characterize an X-ray beam. In this work, we explore the concept in two cases: at-wavelength metrology of 2D parabolic beryllium lenses and a wavefront sensor using a diamond crystal beam splitter.

  20. Soft X-ray spectrum of BL Lacertae object AO 0235+164 as a tracer of elemental abundances at z approximately 0.5

    NASA Technical Reports Server (NTRS)

    Madejski, Greg

    1994-01-01

    We report the soft X-ray spectrum of BL Lac object AO 0235+164, observed with the Einstein Observatory Imaging Proportional Counter (IPC). This object (z = 0.94) has an intervening galaxy (or a protogalactic disk) at z = 0.524 present in the line of sight, producing both radio and optical absorption lines in the background BL Lac continuum. The X-ray spectrum exhibits a substantial soft X-ray cutoff, corresponding to several times that expected from our own Galaxy; we interpret that excess cutoff as due to the intervening galaxy. The comparison of the hydrogen column density inferred from the 21 cm radio data and the X-ray absorption allows, in principle, the determination of the elemental abundances in the intervening galaxy. However, the uncertainties in both the H I spin temperature and X-ray spectral parameters only loosely restrict these abundances to be 2 +/- 1 solar, which even at the lower limit appears higher than that inferred from studies of samples of optical absoprtion-line systems.

  1. 2D and 3D X-ray phase retrieval of multi-material objects using a single defocus distance.

    PubMed

    Beltran, M A; Paganin, D M; Uesugi, K; Kitchen, M J

    2010-03-29

    A method of tomographic phase retrieval is developed for multi-material objects whose components each has a distinct complex refractive index. The phase-retrieval algorithm, based on the Transport-of-Intensity equation, utilizes propagation-based X-ray phase contrast images acquired at a single defocus distance for each tomographic projection. The method requires a priori knowledge of the complex refractive index for each material present in the sample, together with the total projected thickness of the object at each orientation. The requirement of only a single defocus distance per projection simplifies the experimental setup and imposes no additional dose compared to conventional tomography. The algorithm was implemented using phase contrast data acquired at the SPring-8 Synchrotron facility in Japan. The three-dimensional (3D) complex refractive index distribution of a multi-material test object was quantitatively reconstructed using a single X-ray phase-contrast image per projection. The technique is robust in the presence of noise, compared to conventional absorption based tomography.

  2. a Region-Based Multi-Scale Approach for Object-Based Image Analysis

    NASA Astrophysics Data System (ADS)

    Kavzoglu, T.; Yildiz Erdemir, M.; Tonbul, H.

    2016-06-01

    Within the last two decades, object-based image analysis (OBIA) considering objects (i.e. groups of pixels) instead of pixels has gained popularity and attracted increasing interest. The most important stage of the OBIA is image segmentation that groups spectrally similar adjacent pixels considering not only the spectral features but also spatial and textural features. Although there are several parameters (scale, shape, compactness and band weights) to be set by the analyst, scale parameter stands out the most important parameter in segmentation process. Estimating optimal scale parameter is crucially important to increase the classification accuracy that depends on image resolution, image object size and characteristics of the study area. In this study, two scale-selection strategies were implemented in the image segmentation process using pan-sharped Qickbird-2 image. The first strategy estimates optimal scale parameters for the eight sub-regions. For this purpose, the local variance/rate of change (LV-RoC) graphs produced by the ESP-2 tool were analysed to determine fine, moderate and coarse scales for each region. In the second strategy, the image was segmented using the three candidate scale values (fine, moderate, coarse) determined from the LV-RoC graph calculated for whole image. The nearest neighbour classifier was applied in all segmentation experiments and equal number of pixels was randomly selected to calculate accuracy metrics (overall accuracy and kappa coefficient). Comparison of region-based and image-based segmentation was carried out on the classified images and found that region-based multi-scale OBIA produced significantly more accurate results than image-based single-scale OBIA. The difference in classification accuracy reached to 10% in terms of overall accuracy.

  3. Systems and Methods for Imaging of Falling Objects

    NASA Technical Reports Server (NTRS)

    Fallgatter, Cale (Inventor); Garrett, Tim (Inventor)

    2014-01-01

    Imaging of falling objects is described. Multiple images of a falling object can be captured substantially simultaneously using multiple cameras located at multiple angles around the falling object. An epipolar geometry of the captured images can be determined. The images can be rectified to parallelize epipolar lines of the epipolar geometry. Correspondence points between the images can be identified. At least a portion of the falling object can be digitally reconstructed using the identified correspondence points to create a digital reconstruction.

  4. Megavoltage planar and cone-beam imaging with low-Z targets: dependence of image quality improvement on beam energy and patient separation.

    PubMed

    Robar, James L; Connell, Tanner; Huang, Weihong; Kelly, Robin G

    2009-09-01

    The purpose of this study is to investigate the improvement of megavoltage planar and cone-beam CT (CBCT) image quality with the use of low atomic number (Z) external targets in the linear accelerator. In this investigation, two experimental megavoltage imaging beams were generated by using either 3.5 or 7.0 MeV electrons incident on aluminum targets installed above the level of the carousel in a linear accelerator (2100EX, Varian Medical, Inc., Palo Alto, CA). Images were acquired using an amorphous silicon detector panel. Contrast-to-noise ratio (CNR) in planar and CBCT images was measured as a function of dose and a comparison was made between the imaging beams and the standard 6 MV therapy beam. Phantoms of variable diameter were used to examine the loss of contrast due to beam hardening. Porcine imaging was conducted to examine qualitatively the advantages of the low-Z target approach in CBCT. In CBCT imaging CNR increases by factors as high as 2.4 and 4.3 for the 7.0 and 3.5 MeV/Al beams, respectively, compared to images acquired with 6 MV. Similar factors of improvement are observed in planar imaging. For the imaging beams, beam hardening causes a significant loss of the contrast advantage with increasing phantom diameter; however, for the 3.5 MeV/Al beam and a phantom diameter of 25 cm, a contrast advantage remains, with increases of contrast by factors of 1.5 and 3.4 over 6 MV for bone and lung inhale regions, respectively. The spatial resolution is improved slightly in CBCT images for the imaging beams. CBCT images of a porcine cranium demonstrate qualitatively the advantages of the low-Z target approach, showing greater contrast between tissues and improved visibility of fine detail. The use of low-Z external targets in the linear accelerator improves megavoltage planar and CBCT image quality significantly. CNR may be increased by a factor of 4 or greater. Improvement of the spatial resolution is also apparent.

  5. Spectroscopic redshifts and age dating of a first statistical sample of passive galaxies at z 3

    NASA Astrophysics Data System (ADS)

    Daddi, Emanuele

    2017-08-01

    Ultradeep WFC3/G141 observations from one of our past HST programs allowed us to confirm the redshift and measure the age of a quiescent galaxy at z=3. This unique object was found inside a single WFC3 pointing (4 sq. arcmin) suggesting that massive old galaxies even at z 3 are more common than previously thought. The strong correlation observed between evolved stellar populations and a bulge-dominated morphology at least up to z 2 may also imply that the Hubble sequence comes into place at very early times. Guided by the properties of this spectroscopically confirmed z=3 passive galaxy, we have identified a substantial sample of 2.5<z2.5, to detect their 4000A breaks and measure precise redshifts, confirming their identification as distant, quiescent systems (as opposed to dusty star-forming sources), and measuring their stellar ages as well as sizes from the WFC3/F140 ancillary imaging. We have verified that no object of this kind, bright enough to be confirmed spectroscopically, can be found inside existing WFC3 spectroscopy in the CANDELS fields, and that this science cannot be competitively done from the ground. These new observations will push the constraints on the evolution, nature and abundance of passive galaxies to the highest redshifts, and will allow a first detailed picture of the early assembly of the Hubble sequence.z

  6. Object recognition based on Google's reverse image search and image similarity

    NASA Astrophysics Data System (ADS)

    Horváth, András.

    2015-12-01

    Image classification is one of the most challenging tasks in computer vision and a general multiclass classifier could solve many different tasks in image processing. Classification is usually done by shallow learning for predefined objects, which is a difficult task and very different from human vision, which is based on continuous learning of object classes and one requires years to learn a large taxonomy of objects which are not disjunct nor independent. In this paper I present a system based on Google image similarity algorithm and Google image database, which can classify a large set of different objects in a human like manner, identifying related classes and taxonomies.

  7. SUBARU HIGH- z EXPLORATION OF LOW-LUMINOSITY QUASARS (SHELLQs). I. DISCOVERY OF 15 QUASARS AND BRIGHT GALAXIES AT 5.7 < z < 6.9

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Matsuoka, Yoshiki; Kashikawa, Nobunari; Imanishi, Masatoshi

    We report the discovery of 15 quasars and bright galaxies at 5.7 < z < 6.9. This is the initial result from the Subaru High- z Exploration of Low-Luminosity Quasars project, which exploits the exquisite multiband imaging data produced by the Subaru Hyper Suprime-Cam (HSC) Strategic Program survey. The candidate selection is performed by combining several photometric approaches including a Bayesian probabilistic algorithm to reject stars and dwarfs. The spectroscopic identification was carried out with the Gran Telescopio Canarias and the Subaru Telescope for the first 80 deg{sup 2} of the survey footprint. The success rate of our photometric selectionmore » is quite high, approaching 100% at the brighter magnitudes (z {sub AB} < 23.5 mag). Our selection also recovered all the known high- z quasars on the HSC images. Among the 15 discovered objects, six are likely quasars, while the other six with interstellar absorption lines and in some cases narrow emission lines are likely bright Lyman-break galaxies. The remaining three objects have weak continua and very strong and narrow Ly α lines, which may be excited by ultraviolet light from both young stars and quasars. These results indicate that we are starting to see the steep rise of the luminosity function of z ≥ 6 galaxies, compared with that of quasars, at magnitudes fainter than M {sub 1450} ∼ −22 mag or z {sub AB} ∼ 24 mag. Follow-up studies of the discovered objects as well as further survey observations are ongoing.« less

  8. Deep Keck u-Band Imaging of the Hubble Ultra Deep Field: A Catalog of z ~ 3 Lyman Break Galaxies

    NASA Astrophysics Data System (ADS)

    Rafelski, Marc; Wolfe, Arthur M.; Cooke, Jeff; Chen, Hsiao-Wen; Armandroff, Taft E.; Wirth, Gregory D.

    2009-10-01

    We present a sample of 407 z ~ 3 Lyman break galaxies (LBGs) to a limiting isophotal u-band magnitude of 27.6 mag in the Hubble Ultra Deep Field. The LBGs are selected using a combination of photometric redshifts and the u-band drop-out technique enabled by the introduction of an extremely deep u-band image obtained with the Keck I telescope and the blue channel of the Low Resolution Imaging Spectrometer. The Keck u-band image, totaling 9 hr of integration time, has a 1σ depth of 30.7 mag arcsec-2, making it one of the most sensitive u-band images ever obtained. The u-band image also substantially improves the accuracy of photometric redshift measurements of ~50% of the z ~ 3 LBGs, significantly reducing the traditional degeneracy of colors between z ~ 3 and z ~ 0.2 galaxies. This sample provides the most sensitive, high-resolution multi-filter imaging of reliably identified z ~ 3 LBGs for morphological studies of galaxy formation and evolution and the star formation efficiency of gas at high redshift.

  9. Single-beam, dual-view digital holographic interferometry for biomechanical strain measurements of biological objects

    NASA Astrophysics Data System (ADS)

    Pantelić, Dejan V.; Grujić, Dušan Ž.; Vasiljević, Darko M.

    2014-12-01

    We describe a method for dual-view biomechanical strain measurements of highly asymmetrical biological objects, like teeth or bones. By using a spherical mirror, we were able to simultaneously record a digital hologram of the object itself and the mirror image of its (otherwise invisible) rear side. A single laser beam was sufficient to illuminate both sides of the object, and to provide a reference beam. As a result, the system was mechanically very stable, enabling long exposure times (up to 2 min) without the need for vibration isolation. The setup is simple to construct and adjust, and can be used to interferometrically observe any object that is smaller than the mirror diameter. Parallel data processing on a CUDA-enabled (compute unified device architecture) graphics card was used to reconstruct digital holograms and to further correct image distortion. We used the setup to measure the deformation of a tooth due to mastication forces. The finite-element method was used to compare experimental results and theoretical predictions.

  10. Single-beam, dual-view digital holographic interferometry for biomechanical strain measurements of biological objects.

    PubMed

    Pantelić, Dejan V; Grujić, Dušan Ž; Vasiljević, Darko M

    2014-12-01

    We describe a method for dual-view biomechanical strain measurements of highly asymmetrical biological objects, like teeth or bones. By using a spherical mirror, we were able to simultaneously record a digital hologram of the object itself and the mirror image of its (otherwise invisible) rear side. A single laser beam was sufficient to illuminate both sides of the object, and to provide a reference beam. As a result, the system was mechanically very stable, enabling long exposure times (up to 2 min) without the need for vibration isolation. The setup is simple to construct and adjust, and can be used to interferometrically observe any object that is smaller than the mirror diameter. Parallel data processing on a CUDA-enabled (compute unified device architecture) graphics card was used to reconstruct digital holograms and to further correct image distortion. We used the setup to measure the deformation of a tooth due to mastication forces. The finite-element method was used to compare experimental results and theoretical predictions.

  11. Application of two-dimensional crystallography and image processing to atomic resolution Z-contrast images.

    PubMed

    Morgan, David G; Ramasse, Quentin M; Browning, Nigel D

    2009-06-01

    Zone axis images recorded using high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM or Z-contrast imaging) reveal the atomic structure with a resolution that is defined by the probe size of the microscope. In most cases, the full images contain many sub-images of the crystal unit cell and/or interface structure. Thanks to the repetitive nature of these images, it is possible to apply standard image processing techniques that have been developed for the electron crystallography of biological macromolecules and have been used widely in other fields of electron microscopy for both organic and inorganic materials. These methods can be used to enhance the signal-to-noise present in the original images, to remove distortions in the images that arise from either the instrumentation or the specimen itself and to quantify properties of the material in ways that are difficult without such data processing. In this paper, we describe briefly the theory behind these image processing techniques and demonstrate them for aberration-corrected, high-resolution HAADF-STEM images of Si(46) clathrates developed for hydrogen storage.

  12. Redshift determination of the BL Lac object 3C 66A by the detection of its host galaxy cluster at z = 0.340

    NASA Astrophysics Data System (ADS)

    Torres-Zafra, Juanita; Cellone, Sergio A.; Buzzoni, Alberto; Andruchow, Ileana; Portilla, José G.

    2018-03-01

    The BL Lac object 3C 66A is one of the most luminous extragalactic sources at TeV γ-rays (very high energy, i.e. E > 100 GeV). Since TeV γ-ray radiation is absorbed by the extragalactic background light (EBL), it is crucial to know the redshift of the source in order to reconstruct its original spectral energy distribution, as well as to constrain EBL models. However, the optical spectrum of this BL Lac is almost featureless, so a direct measurement of z is very difficult; in fact, the published redshift value for this source (z = 0.444) has been strongly questioned. Based on EBL absorption arguments, several constraints to its redshift, in the range 0.096 < z < 0.5, were proposed. Since these active galactic nuclei (AGNs) are hosted, typically, in early-type galaxies that are members of groups or clusters, we have analysed spectro-photometrically the environment of 3C 66A, with the goal of finding the galaxy group hosting this blazar. This study was made using optical images of a 5.5 × 5.5 arcmin2 field centred on the blazar, and spectra of 24 sources obtained with Gemini/GMOS-N multi-object spectroscopy. We found spectroscopic evidence of two galaxy groups along the blazar's line of sight: one at z ≃ 0.020 and the second one at z ≃ 0.340. The first one is consistent with a known foreground structure, while the second group presented here has six spectroscopically confirmed members. Their location along a red sequence in the colour-magnitude diagram allows us to identify 34 additional candidate members of the more distant group. The blazar's spectrum shows broad absorption features that we identify as arising in the intergalactic medium, thus allowing us to tentatively set a redshift lower limit at z_3C66A ≳ 0.33. As a consequence, we propose that 3C 66A is hosted in a galaxy that belongs to a cluster at z = 0.340.

  13. Image Discrimination Predictions of a Single Channel Model with Contrast Gain Control

    NASA Technical Reports Server (NTRS)

    Ahumada, Albert J., Jr.; Null, Cynthia H.

    1995-01-01

    Image discrimination models predict the number of just-noticeable-differences between two images. We report the predictions of a single channel model with contrast masking for a range of standard discrimination experiments. Despite its computational simplicity, this model has performed as well as a multiple channel model in an object detection task.

  14. Lucky Imaging: Improved Localization Accuracy for Single Molecule Imaging

    PubMed Central

    Cronin, Bríd; de Wet, Ben; Wallace, Mark I.

    2009-01-01

    We apply the astronomical data-analysis technique, Lucky imaging, to improve resolution in single molecule fluorescence microscopy. We show that by selectively discarding data points from individual single-molecule trajectories, imaging resolution can be improved by a factor of 1.6 for individual fluorophores and up to 5.6 for more complex images. The method is illustrated using images of fluorescent dye molecules and quantum dots, and the in vivo imaging of fluorescently labeled linker for activation of T cells. PMID:19348772

  15. Improved space object detection using short-exposure image data with daylight background.

    PubMed

    Becker, David; Cain, Stephen

    2018-05-10

    Space object detection is of great importance in the highly dependent yet competitive and congested space domain. The detection algorithms employed play a crucial role in fulfilling the detection component in the space situational awareness mission to detect, track, characterize, and catalog unknown space objects. Many current space detection algorithms use a matched filter or a spatial correlator on long-exposure data to make a detection decision at a single pixel point of a spatial image based on the assumption that the data follow a Gaussian distribution. Long-exposure imaging is critical to detection performance in these algorithms; however, for imaging under daylight conditions, it becomes necessary to create a long-exposure image as the sum of many short-exposure images. This paper explores the potential for increasing detection capabilities for small and dim space objects in a stack of short-exposure images dominated by a bright background. The algorithm proposed in this paper improves the traditional stack and average method of forming a long-exposure image by selectively removing short-exposure frames of data that do not positively contribute to the overall signal-to-noise ratio of the averaged image. The performance of the algorithm is compared to a traditional matched filter detector using data generated in MATLAB as well as laboratory-collected data. The results are illustrated on a receiver operating characteristic curve to highlight the increased probability of detection associated with the proposed algorithm.

  16. Imaging of single retinal ganglion cell with differential interference contrast microscopy (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Oh, Juyeong; Kim, Yu Jeong; Kim, Chul-Ki; Lee, Taik Jin; Seo, Mina; Lee, Seok; Woo, Deok Ha; Jun, Seong Chan; Park, Ki-Ho; Kim, Seok Hwan; Kim, Jae Hun

    2017-02-01

    Glaucoma is a progressive optic neuropathy, characterized by the selective loss of retinal ganglion cells (RGCs). Therefore, monitoring the change of number or morphology of RGC is essential for the early detection as well as investigation of pathophysiology of glaucoma. Since RGC layer is transparent and hyporeflective, the direct optical visualization of RGCs has not been successful so far. Therefore, glaucoma evaluation mostly depends on indirect diagnostic methods such as the evaluation of optic disc morphology or retinal nerve fiber layer thickness measurement by optical coherence tomography. We have previously demonstrated single photoreceptor cell imaging with differential interference contrast (DIC) microscopy. Herein, we successfully visualized single RGC using DIC microscopy. Since RGC layer is much less reflective than photoreceptor layer, various techniques including the control of light wavelength and bandwidth using a tunable band pass filter were adopted to reduce the chromatic aberration in z-axis for higher and clearer resolution. To verify that the imaged cells were the RGCs, the flat-mounted retina of Sprague-Dawley rat, in which the RGCs were retrogradely labeled with fluorescence, was observed by both fluorescence and DIC microscopies for direct comparison. We have confirmed that the cell images obtained by fluorescence microscopy were perfectly matched with cell images by DIC microscopy. As conclusion, we have visualized single RGC with DIC microscopy, and confirmed with fluorescence microscopy.

  17. A z-gradient array for simultaneous multi-slice excitation with a single-band RF pulse.

    PubMed

    Ertan, Koray; Taraghinia, Soheil; Sadeghi, Alireza; Atalar, Ergin

    2018-07-01

    Multi-slice radiofrequency (RF) pulses have higher specific absorption rates, more peak RF power, and longer pulse durations than single-slice RF pulses. Gradient field design techniques using a z-gradient array are investigated for exciting multiple slices with a single-band RF pulse. Two different field design methods are formulated to solve for the required current values of the gradient array elements for the given slice locations. The method requirements are specified, optimization problems are formulated for the minimum current norm and an analytical solution is provided. A 9-channel z-gradient coil array driven by independent, custom-designed gradient amplifiers is used to validate the theory. Performance measures such as normalized slice thickness error, gradient strength per unit norm current, power dissipation, and maximum amplitude of the magnetic field are provided for various slice locations and numbers of slices. Two and 3 slices are excited by a single-band RF pulse in simulations and phantom experiments. The possibility of multi-slice excitation with a single-band RF pulse using a z-gradient array is validated in simulations and phantom experiments. Magn Reson Med 80:400-412, 2018. © 2017 International Society for Magnetic Resonance in Medicine. © 2017 International Society for Magnetic Resonance in Medicine.

  18. Evaluation of image quality in terahertz pulsed imaging using test objects.

    PubMed

    Fitzgerald, A J; Berry, E; Miles, R E; Zinovev, N N; Smith, M A; Chamberlain, J M

    2002-11-07

    As with other imaging modalities, the performance of terahertz (THz) imaging systems is limited by factors of spatial resolution, contrast and noise. The purpose of this paper is to introduce test objects and image analysis methods to evaluate and compare THz image quality in a quantitative and objective way, so that alternative terahertz imaging system configurations and acquisition techniques can be compared, and the range of image parameters can be assessed. Two test objects were designed and manufactured, one to determine the modulation transfer functions (MTF) and the other to derive image signal to noise ratio (SNR) at a range of contrasts. As expected the higher THz frequencies had larger MTFs, and better spatial resolution as determined by the spatial frequency at which the MTF dropped below the 20% threshold. Image SNR was compared for time domain and frequency domain image parameters and time delay based images consistently demonstrated higher SNR than intensity based parameters such as relative transmittance because the latter are more strongly affected by the sources of noise in the THz system such as laser fluctuations and detector shot noise.

  19. Current scaling of radiated power for 40-mm diameter single wire arrays on Z

    NASA Astrophysics Data System (ADS)

    Nash, T. J.; Cuneo, M. E.; Spielman, R. B.; Chandler, G. A.; Leeper, R. J.; Seaman, J. F.; McGurn, J.; Lazier, S.; Torres, J.; Jobe, D.; Gilliland, T.; Nielsen, D.; Hawn, R.; Bailey, J. E.; Lake, P.; Carlson, A. L.; Seamen, H.; Moore, T.; Smelser, R.; Pyle, J.; Wagoner, T. C.; LePell, P. D.; Deeney, C.; Douglas, M. R.; McDaniel, D.; Struve, K.; Mazarakis, M.; Stygar, W. A.

    2004-11-01

    In order to estimate the radiated power that can be expected from the next-generation Z-pinch driver such as ZR at 28 MA, current-scaling experiments have been conducted on the 20 MA driver Z. We report on the current scaling of single 40 mm diameter tungsten 240 wire arrays with a fixed 110 ns implosion time. The wire diameter is decreased in proportion to the load current. Reducing the charge voltage on the Marx banks reduces the load current. On one shot, firing only three of the four levels of the Z machine further reduced the load current. The radiated energy scaled as the current squared as expected but the radiated power scaled as the current to the 3.52±0.42 power due to increased x-ray pulse width at lower current. As the current is reduced, the rise time of the x-ray pulse increases and at the lowest current value of 10.4 MA, a shoulder appears on the leading edge of the x-ray pulse. In order to determine the nature of the plasma producing the leading edge of the x-ray pulse at low currents further shots were taken with an on-axis aperture to view on-axis precursor plasma. This aperture appeared to perturb the pinch in a favorable manner such that with the aperture in place there was no leading edge to the x-ray pulses at lower currents and the radiated power scaled as the current squared ±0.75. For a full-current shot we will present x-ray images that show precursor plasma emitting on-axis 77 ns before the main x-ray burst.

  20. Estimation of object motion parameters from noisy images.

    PubMed

    Broida, T J; Chellappa, R

    1986-01-01

    An approach is presented for the estimation of object motion parameters based on a sequence of noisy images. The problem considered is that of a rigid body undergoing unknown rotational and translational motion. The measurement data consists of a sequence of noisy image coordinates of two or more object correspondence points. By modeling the object dynamics as a function of time, estimates of the model parameters (including motion parameters) can be extracted from the data using recursive and/or batch techniques. This permits a desired degree of smoothing to be achieved through the use of an arbitrarily large number of images. Some assumptions regarding object structure are presently made. Results are presented for a recursive estimation procedure: the case considered here is that of a sequence of one dimensional images of a two dimensional object. Thus, the object moves in one transverse dimension, and in depth, preserving the fundamental ambiguity of the central projection image model (loss of depth information). An iterated extended Kalman filter is used for the recursive solution. Noise levels of 5-10 percent of the object image size are used. Approximate Cramer-Rao lower bounds are derived for the model parameter estimates as a function of object trajectory and noise level. This approach may be of use in situations where it is difficult to resolve large numbers of object match points, but relatively long sequences of images (10 to 20 or more) are available.

  1. Using SAR Interferograms and Coherence Images for Object-Based Delineation of Unstable Slopes

    NASA Astrophysics Data System (ADS)

    Friedl, Barbara; Holbling, Daniel

    2015-05-01

    This study uses synthetic aperture radar (SAR) interferometric products for the semi-automated identification and delineation of unstable slopes and active landslides. Single-pair interferograms and coherence images are therefore segmented and classified in an object-based image analysis (OBIA) framework. The rule-based classification approach has been applied to landslide-prone areas located in Taiwan and Southern Germany. The semi-automatically obtained results were validated against landslide polygons derived from manual interpretation.

  2. CLASH: THREE STRONGLY LENSED IMAGES OF A CANDIDATE z Almost-Equal-To 11 GALAXY

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Coe, Dan; Postman, Marc; Bradley, Larry

    2013-01-01

    We present a candidate for the most distant galaxy known to date with a photometric redshift of z = 10.7{sup +0.6} {sub -0.4} (95% confidence limits; with z < 9.5 galaxies of known types ruled out at 7.2{sigma}). This J-dropout Lyman break galaxy, named MACS0647-JD, was discovered as part of the Cluster Lensing and Supernova survey with Hubble (CLASH). We observe three magnified images of this galaxy due to strong gravitational lensing by the galaxy cluster MACSJ0647.7+7015 at z = 0.591. The images are magnified by factors of {approx}80, 7, and 2, with the brighter two observed at {approx}26th magnitudemore » AB ({approx}0.15 {mu}Jy) in the WFC3/IR F160W filter ({approx}1.4-1.7 {mu}m) where they are detected at {approx}>12{sigma}. All three images are also confidently detected at {approx}>6{sigma} in F140W ({approx}1.2-1.6 {mu}m), dropping out of detection from 15 lower wavelength Hubble Space Telescope filters ({approx}0.2-1.4 {mu}m), and lacking bright detections in Spitzer/IRAC 3.6 {mu}m and 4.5 {mu}m imaging ({approx}3.2-5.0 {mu}m). We rule out a broad range of possible lower redshift interlopers, including some previously published as high-redshift candidates. Our high-redshift conclusion is more conservative than if we had neglected a Bayesian photometric redshift prior. Given CLASH observations of 17 high-mass clusters to date, our discoveries of MACS0647-JD at z {approx} 10.8 and MACS1149-JD at z {approx} 9.6 are consistent with a lensed luminosity function extrapolated from lower redshifts. This would suggest that low-luminosity galaxies could have reionized the universe. However, given the significant uncertainties based on only two galaxies, we cannot yet rule out the sharp drop-off in number counts at z {approx}> 10 suggested by field searches.« less

  3. CognitionMaster: an object-based image analysis framework

    PubMed Central

    2013-01-01

    Background Automated image analysis methods are becoming more and more important to extract and quantify image features in microscopy-based biomedical studies and several commercial or open-source tools are available. However, most of the approaches rely on pixel-wise operations, a concept that has limitations when high-level object features and relationships between objects are studied and if user-interactivity on the object-level is desired. Results In this paper we present an open-source software that facilitates the analysis of content features and object relationships by using objects as basic processing unit instead of individual pixels. Our approach enables also users without programming knowledge to compose “analysis pipelines“ that exploit the object-level approach. We demonstrate the design and use of example pipelines for the immunohistochemistry-based cell proliferation quantification in breast cancer and two-photon fluorescence microscopy data about bone-osteoclast interaction, which underline the advantages of the object-based concept. Conclusions We introduce an open source software system that offers object-based image analysis. The object-based concept allows for a straight-forward development of object-related interactive or fully automated image analysis solutions. The presented software may therefore serve as a basis for various applications in the field of digital image analysis. PMID:23445542

  4. Single particle maximum likelihood reconstruction from superresolution microscopy images

    PubMed Central

    Verdier, Timothée; Gunzenhauser, Julia; Manley, Suliana; Castelnovo, Martin

    2017-01-01

    Point localization superresolution microscopy enables fluorescently tagged molecules to be imaged beyond the optical diffraction limit, reaching single molecule localization precisions down to a few nanometers. For small objects whose sizes are few times this precision, localization uncertainty prevents the straightforward extraction of a structural model from the reconstructed images. We demonstrate in the present work that this limitation can be overcome at the single particle level, requiring no particle averaging, by using a maximum likelihood reconstruction (MLR) method perfectly suited to the stochastic nature of such superresolution imaging. We validate this method by extracting structural information from both simulated and experimental PALM data of immature virus-like particles of the Human Immunodeficiency Virus (HIV-1). MLR allows us to measure the radii of individual viruses with precision of a few nanometers and confirms the incomplete closure of the viral protein lattice. The quantitative results of our analysis are consistent with previous cryoelectron microscopy characterizations. Our study establishes the framework for a method that can be broadly applied to PALM data to determine the structural parameters for an existing structural model, and is particularly well suited to heterogeneous features due to its single particle implementation. PMID:28253349

  5. Imaging Young Stellar Objects with VLTi/PIONIER

    NASA Astrophysics Data System (ADS)

    Kluska, J.; Malbet, F.; Berger, J.-P.; Benisty, M.; Lazareff, B.; Le Bouquin, J.-B.; Baron, F.; Dominik, C.; Isella, A.; Juhasz, A.; Kraus, S.; Lachaume, R.; Ménard, F.; Millan-Gabet, R.; Monnier, J.; Pinte, C.; Soulez, F.; Tallon, M.; Thi, W.-F.; Thiébaut, É.; Zins, G.

    2014-04-01

    Optical interferometry imaging is designed to help us to reveal complex astronomical sources without a prior model. Among these complex objects are the young stars and their environments, which have a typical morphology with a point-like source, surrounded by circumstellar material with unknown morphology. To image them, we have developed a numerical method that removes completely the stellar point source and reconstructs the rest of the image, using the differences in the spectral behavior between the star and its circumstellar material. We aim to reveal the first Astronomical Units of these objects where many physical phenomena could interplay: the dust sublimation causing a puffed-up inner rim, a dusty halo, a dusty wind or an inner gaseous component. To investigate more deeply these regions, we carried out the first Large Program survey of HAeBe stars with two main goals: statistics on the geometry of these objects at the first astronomical unit scale and imaging their very close environment. The images reveal the environment, which is not polluted by the star and allows us to derive the best fit for the flux ratio and the spectral slope. We present the first images from this survey and the application of the imaging method on other astronomical objects.

  6. Search for pair produced stable singly charged heavy particles in Z 0 decays

    NASA Astrophysics Data System (ADS)

    Akrawy, M. Z.; Alexander, G.; Allison, J.; Allport, P. P.; Anderson, K. J.; Armitage, J. C.; Arnison, G. T. J.; Ashton, P.; Azuelos, G.; Baines, J. T. M.; Ball, A. H.; Banks, J.; Barker, G. J.; Barlow, R. J.; Batley, J. R.; Beck, A.; Becker, J.; Behnke, T.; Bell, K. W.; Bella, G.; Bethke, S.; Biebel, O.; Binder, U.; Bloodworth, I. J.; Bock, P.; Breuker, H.; Brown, R. M.; Brun, R.; Buijs, A.; Burckhart, H. J.; Capiluppi, P.; Carnegie, R. K.; Carter, A. A.; Carter, J. R.; Chang, C. Y.; Charlton, D. G.; Chrin, J. T. M.; Clarke, P. E. L.; Cohen, I.; Collins, W. J.; Conboy, J. E.; Couch, M.; Coupland, M.; Cuffiani, M.; Dado, S.; Dallavalle, G. M.; Debu, P.; Deninno, M. M.; Dieckmann, A.; Dittmar, M.; Dixit, M. S.; Duchovni, E.; Duerdoth, I. P.; Dumas, D. J. P.; El Mamouni, H.; Elcombe, P. A.; Estabrooks, P. G.; Etzion, E.; Fabbri, F.; Farthouat, P.; Fischer, H. M.; Fong, D. G.; French, M. T.; Fukunaga, C.; Gaidot, A.; Ganel, O.; Gary, J. W.; Gascon, J.; Geddes, N. I.; Gee, C. N. P.; Geich-Gimbel, C.; Gensler, S. W.; Gentit, F. X.; Giacomelli, G.; Gibson, V.; Gibson, W. R.; Gillies, J. D.; Goldberg, J.; Goodrick, M. J.; Gorn, W.; Granite, D.; Gross, E.; Grunhaus, J.; Hagedorn, H.; Hagemann, J.; Hansroul, M.; Hargrove, C. K.; Harrus, I.; Hart, J.; Hattersley, P. M.; Hauschild, M.; Hawkes, C. M.; Heflin, E.; Hemingway, R. J.; Heuer, R. D.; Hill, J. C.; Hillier, S. J.; Ho, C.; Hobbs, J. D.; Hobson, P. R.; Hochman, D.; Holl, B.; Homer, R. J.; Hou, S. R.; Howarth, C. P.; Hughes-Jones, R. E.; Humbert, R.; Igo-Kemenes, P.; Ihssen, H.; Imrie, D. C.; Janissen, L.; Jawahery, A.; Jeffreys, P. W.; Jeremie, H.; Jimack, M.; Jobes, M.; Jones, R. W. L.; Jovanovic, P.; Karlen, D.; Kawagoe, K.; Kawamoto, T.; Kellogg, R. G.; Kennedy, B. W.; Kleinwort, C.; Klem, D. E.; Knop, G.; Kobayashi, T.; Kokott, T. P.; Köpke, L.; Kowalewski, R.; Kreutzmann, H.; Kroll, J.; Kuwano, M.; Kyberd, P.; Lafferty, G. D.; Lamarche, F.; Larson, W. J.; Layter, J. G.; Le Du, P.; Leblanc, P.; Lee, A. M.; Lehto, M. H.; Lellouch, D.; Lennert, P.; Lessard, L.; Levinson, L.; Lloyd, S. L.; Loebinger, F. K.; Lorah, J. M.; Lorazo, B.; Losty, M. J.; Ludwig, J.; Ma, J.; Macbeth, A. A.; Mannelli, M.; Marcellini, S.; Maringer, G.; Martin, A. J.; Martin, J. P.; Mashimo, T.; Mättig, P.; Maur, U.; McMahon, T. J.; McNutt, J. R.; Meijers, F.; Menszner, D.; Merritt, F. S.; Mes, H.; Michelini, A.; Middleton, R. P.; Mikenberg, G.; Mildenberger, J.; Miller, D. J.; Milstene, C.; Minowa, M.; Mohr, W.; Montanari, A.; Mori, T.; Moss, M. W.; Murphy, P. G.; Murray, W. J.; Nellen, B.; Nguyen, H. H.; Nozaki, M.; O'Dowd, A. J. P.; O'Neale, S. W.; O'Neill, B. P.; Oakham, F. G.; Odorici, F.; Ogg, M.; Oh, H.; Oreglia, M. J.; Orito, S.; Pansart, J. P.; Patrick, G. N.; Pawley, S. J.; Pfister, P.; Pilcher, J. E.; Pinfold, J. L.; Plane, D. E.; Poli, B.; Pouladdej, A.; Prebys, E.; Pritchard, T. W.; Quast, G.; Raab, J.; Redmond, M. W.; Rees, D. L.; Regimbald, M.; Riles, K.; Roach, C. M.; Robins, S. A.; Rollnik, A.; Roney, J. M.; Rossberg, S.; Rossi, A. M.; Routenburg, P.; Runge, K.; Runolfsson, O.; Sanghera, S.; Sansum, R. A.; Sasaki, M.; Saunders, B. J.; Schaile, A. D.; Schaile, O.; Schappert, W.; Scharff-Hansen, P.; Schreiber, S.; Schwarz, J.; Shapira, A.; Shen, B. C.; Sherwood, P.; Simon, A.; Singh, P.; Siroli, G. P.; Skuja, A.; Smith, A. M.; Smith, T. J.; Snow, G. A.; Springer, R. W.; Sproston, M.; Stephens, K.; Stier, H. E.; Stroehmer, R.; Strom, D.; Takeda, H.; Takeshita, T.; Thackray, N. J.; Tsukamoto, T.; Turner, M. F.; Tysarczyk-Niemeyer, G.; Van den plas, D.; VanDalen, G. J.; Vasseur, G.; Virtue, C. J.; von der Schmitt, H.; von Krogh, J.; Wagner, A.; Wahl, C.; Walker, J. P.; Ward, C. P.; Ward, D. R.; Watkins, P. M.; Watson, A. T.; Watson, N. K.; Weber, M.; Weisz, S.; Wells, P. S.; Wermes, N.; Weymann, M.; Wilson, G. W.; Wilson, J. A.; Wingerter, I.; Winterer, V.-H.; Wood, N. C.; Wotton, S.; Wuensch, B.; Wyatt, T. R.; Yaari, R.; Yang, Y.; Yekutieli, G.; Yoshida, T.; Zeuner, W.; Zorn, G. T.; OPAL Collaboration

    1990-12-01

    A direct search for the exclusive pair production of stable singly charged heavy (SCH) particles in Z 0 decays at the LEP e +e - collider is presented. In a scan around the Z 0 resonance of 0.4 pb - integrated luminosity, no evidence is seen for the production of slow-moving charged particles as measured by their time-of-flight. We set an upper limit of 1 × 10 -3 on the Z 0 branching ratio into a pair of SCH fermions in the mass range 29-40 GeV/ c2, corresponding to a 3 MeV upper limit on such a contribution to the total width of the Z 0. We exclude a fourth generation SCH lepton in the mass range 18.5-42.8 GeV/ c2. We also exclude a stable supersymmetric partner of the right-handed lepton, l˜R, in the mass range 21.5-38.8 GeV/ c2. All limits are at 95% confidence level.

  7. Imaging the cold molecular gas in SDSS J1148 + 5251 at z = 6.4

    NASA Astrophysics Data System (ADS)

    Stefan, Irina I.; Carilli, Chris L.; Wagg, Jeff; Walter, Fabian; Riechers, Dominik A.; Bertoldi, Frank; Green, David A.; Fan, Xiaohui; Menten, Karl; Wang, Ran

    2015-08-01

    We present Karl G. Jansky Very Large Array (VLA) observations of the CO (J = 2 → 1) line emission towards the z = 6.419 quasar SDSS J114816.64 + 525150.3 (J1148 + 5251). The molecular gas is found to be marginally resolved with a major axis of 0.9 arcsec (consistent with previous size measurements of the CO (J = 7 → 6) emission). We observe tentative evidence for extended line emission towards the south-west on a scale of ˜1.4 arcsec, but this is only detected at 3.3σ significance and should be confirmed. The position of the molecular emission region is in excellent agreement with previous detections of low-frequency radio continuum emission as well as [C II] line and thermal dust continuum emission. These CO (J = 2 → 1) observations provide an anchor for the low-excitation part of the molecular line spectral energy distribution. We find no evidence for extended low-excitation component, neither in the spectral line energy distribution nor the image. We fit a single kinetic gas temperature model of 50 K. We revisit the gas and dynamical masses in light of this new detection of a low-order transition of CO, and confirm previous findings that there is no extended reservoir of cold molecular gas in J1148 + 5251, and that the source departs substantially from the low-z relationship between black hole mass and bulge mass. Hence, the characteristics of J1148 + 5251 at z = 6.419 are very similar to z ˜ 2 quasars, in the lack of a diffuse cold gas reservoir and kpc-size compactness of the star-forming region.

  8. Full-color digitized holography for large-scale holographic 3D imaging of physical and nonphysical objects.

    PubMed

    Matsushima, Kyoji; Sonobe, Noriaki

    2018-01-01

    Digitized holography techniques are used to reconstruct three-dimensional (3D) images of physical objects using large-scale computer-generated holograms (CGHs). The object field is captured at three wavelengths over a wide area at high densities. Synthetic aperture techniques using single sensors are used for image capture in phase-shifting digital holography. The captured object field is incorporated into a virtual 3D scene that includes nonphysical objects, e.g., polygon-meshed CG models. The synthetic object field is optically reconstructed as a large-scale full-color CGH using red-green-blue color filters. The CGH has a wide full-parallax viewing zone and reconstructs a deep 3D scene with natural motion parallax.

  9. Attenuated total reflectance FT-IR imaging and quantitative energy dispersive-electron probe X-ray microanalysis techniques for single particle analysis of atmospheric aerosol particles.

    PubMed

    Ryu, JiYeon; Ro, Chul-Un

    2009-08-15

    This work demonstrates the practical applicability of the combined use of attenuated total reflectance (ATR) FT-IR imaging and low-Z particle electron probe X-ray microanalysis (EPMA) techniques for the characterization of individual aerosol particles. These two single particle analytical techniques provide complementary information on the physicochemical characteristics of the same individual particles, that is, the low-Z particle EPMA for the information on the morphology and elemental concentration and the ATR-FT-IR imaging on the functional group, molecular species, and crystal structure. It was confirmed that the ATR-FT-IR imaging technique can provide sufficient FT-IR absorption signals to perform molecular speciation of individual particles of micrometer size when applied to artificially generated aerosol particles such as ascorbic acid and NaNO(3) aerosols. An exemplar indoor atmospheric aerosol sample was investigated to demonstrate the practical feasibility of the combined application of ATR-FT-IR imaging and low-Z particle EPMA techniques for the characterization of individual airborne particles.

  10. A Multi-Objective Decision Making Approach for Solving the Image Segmentation Fusion Problem.

    PubMed

    Khelifi, Lazhar; Mignotte, Max

    2017-08-01

    Image segmentation fusion is defined as the set of methods which aim at merging several image segmentations, in a manner that takes full advantage of the complementarity of each one. Previous relevant researches in this field have been impeded by the difficulty in identifying an appropriate single segmentation fusion criterion, providing the best possible, i.e., the more informative, result of fusion. In this paper, we propose a new model of image segmentation fusion based on multi-objective optimization which can mitigate this problem, to obtain a final improved result of segmentation. Our fusion framework incorporates the dominance concept in order to efficiently combine and optimize two complementary segmentation criteria, namely, the global consistency error and the F-measure (precision-recall) criterion. To this end, we present a hierarchical and efficient way to optimize the multi-objective consensus energy function related to this fusion model, which exploits a simple and deterministic iterative relaxation strategy combining the different image segments. This step is followed by a decision making task based on the so-called "technique for order performance by similarity to ideal solution". Results obtained on two publicly available databases with manual ground truth segmentations clearly show that our multi-objective energy-based model gives better results than the classical mono-objective one.

  11. Object detection from images obtained through underwater turbulence medium

    NASA Astrophysics Data System (ADS)

    Furhad, Md. Hasan; Tahtali, Murat; Lambert, Andrew

    2017-09-01

    Imaging through underwater experiences severe distortions due to random fluctuations of temperature and salinity in water, which produces underwater turbulence through diffraction limited blur. Lights reflecting from objects perturb and attenuate contrast, making the recognition of objects of interest difficult. Thus, the information available for detecting underwater objects of interest becomes a challenging task as they have inherent confusion among the background, foreground and other image properties. In this paper, a saliency-based approach is proposed to detect the objects acquired through an underwater turbulent medium. This approach has drawn attention among a wide range of computer vision applications, such as image retrieval, artificial intelligence, neuro-imaging and object detection. The image is first processed through a deblurring filter. Next, a saliency technique is used on the image for object detection. In this step, a saliency map that highlights the target regions is generated and then a graph-based model is proposed to extract these target regions for object detection.

  12. Precise weak lensing constraints from deep high-resolution Ks images: VLT/HAWK-I analysis of the super-massive galaxy cluster RCS2 J 232727.7-020437 at z = 0.70

    NASA Astrophysics Data System (ADS)

    Schrabback, Tim; Schirmer, Mischa; van der Burg, Remco F. J.; Hoekstra, Henk; Buddendiek, Axel; Applegate, Douglas; Bradač, Maruša; Eifler, Tim; Erben, Thomas; Gladders, Michael D.; Hernández-Martín, Beatriz; Hildebrandt, Hendrik; Hoag, Austin; Klaes, Dominik; von der Linden, Anja; Marchesini, Danilo; Muzzin, Adam; Sharon, Keren; Stefanon, Mauro

    2018-03-01

    We demonstrate that deep good-seeing VLT/HAWK-I Ks images complemented with g + z-band photometry can yield a sensitivity for weak lensing studies of massive galaxy clusters at redshifts 0.7 ≲ z ≲ 1.1, which is almost identical to the sensitivity of HST/ACS mosaics of single-orbit depth. Key reasons for this good performance are the excellent image quality frequently achievable for Ks imaging from the ground, a highly effective photometric selection of background galaxies, and a galaxy ellipticity dispersion that is noticeably lower than for optically observed high-redshift galaxy samples. Incorporating results from the 3D-HST and UltraVISTA surveys we also obtained a more accurate calibration of the source redshift distribution than previously achieved for similar optical weak lensing data sets. Here we studied the extremely massive galaxy cluster RCS2 J232727.7-020437 (z = 0.699), combining deep VLT/HAWK-I Ks images (point spread function with a 0.''35 full width at half maximum) with LBT/LBC photometry. The resulting weak lensing mass reconstruction suggests that the cluster consists of a single overdensity, which is detected with a peak significance of 10.1σ. We constrained the cluster mass to M200c/(1015 M⊙) = 2.06-0.26+0.28(stat.) ± 0.12(sys.) assuming a spherical Navarro, Frenk & White model and simulation-based priors on the concentration, making it one of the most massive galaxy clusters known in the z ≳ 0.7 Universe. We also cross-checked the HAWK-I measurements through an analysis of overlapping HST/ACS images, yielding fully consistent estimates of the lensing signal. Based on observations conducted with the ESO Very Large Telescope, the Large Binocular Telescope, and the NASA/ESA Hubble Space Telescope, as detailed in the acknowledgements.

  13. Classifying four-category visual objects using multiple ERP components in single-trial ERP.

    PubMed

    Qin, Yu; Zhan, Yu; Wang, Changming; Zhang, Jiacai; Yao, Li; Guo, Xiaojuan; Wu, Xia; Hu, Bin

    2016-08-01

    Object categorization using single-trial electroencephalography (EEG) data measured while participants view images has been studied intensively. In previous studies, multiple event-related potential (ERP) components (e.g., P1, N1, P2, and P3) were used to improve the performance of object categorization of visual stimuli. In this study, we introduce a novel method that uses multiple-kernel support vector machine to fuse multiple ERP component features. We investigate whether fusing the potential complementary information of different ERP components (e.g., P1, N1, P2a, and P2b) can improve the performance of four-category visual object classification in single-trial EEGs. We also compare the classification accuracy of different ERP component fusion methods. Our experimental results indicate that the classification accuracy increases through multiple ERP fusion. Additional comparative analyses indicate that the multiple-kernel fusion method can achieve a mean classification accuracy higher than 72 %, which is substantially better than that achieved with any single ERP component feature (55.07 % for the best single ERP component, N1). We compare the classification results with those of other fusion methods and determine that the accuracy of the multiple-kernel fusion method is 5.47, 4.06, and 16.90 % higher than those of feature concatenation, feature extraction, and decision fusion, respectively. Our study shows that our multiple-kernel fusion method outperforms other fusion methods and thus provides a means to improve the classification performance of single-trial ERPs in brain-computer interface research.

  14. Image Retrieval Method for Multiscale Objects from Optical Colonoscopy Images

    PubMed Central

    Sakanashi, Hidenori; Takahashi, Eiichi; Murakawa, Masahiro; Aoki, Hiroshi; Takeuchi, Ken; Suzuki, Yasuo

    2017-01-01

    Optical colonoscopy is the most common approach to diagnosing bowel diseases through direct colon and rectum inspections. Periodic optical colonoscopy examinations are particularly important for detecting cancers at early stages while still treatable. However, diagnostic accuracy is highly dependent on both the experience and knowledge of the medical doctor. Moreover, it is extremely difficult, even for specialist doctors, to detect the early stages of cancer when obscured by inflammations of the colonic mucosa due to intractable inflammatory bowel diseases, such as ulcerative colitis. Thus, to assist the UC diagnosis, it is necessary to develop a new technology that can retrieve similar cases of diagnostic target image from cases in the past that stored the diagnosed images with various symptoms of colonic mucosa. In order to assist diagnoses with optical colonoscopy, this paper proposes a retrieval method for colonoscopy images that can cope with multiscale objects. The proposed method can retrieve similar colonoscopy images despite varying visible sizes of the target objects. Through three experiments conducted with real clinical colonoscopy images, we demonstrate that the method is able to retrieve objects of any visible size and any location at a high level of accuracy. PMID:28255295

  15. Image Retrieval Method for Multiscale Objects from Optical Colonoscopy Images.

    PubMed

    Nosato, Hirokazu; Sakanashi, Hidenori; Takahashi, Eiichi; Murakawa, Masahiro; Aoki, Hiroshi; Takeuchi, Ken; Suzuki, Yasuo

    2017-01-01

    Optical colonoscopy is the most common approach to diagnosing bowel diseases through direct colon and rectum inspections. Periodic optical colonoscopy examinations are particularly important for detecting cancers at early stages while still treatable. However, diagnostic accuracy is highly dependent on both the experience and knowledge of the medical doctor. Moreover, it is extremely difficult, even for specialist doctors, to detect the early stages of cancer when obscured by inflammations of the colonic mucosa due to intractable inflammatory bowel diseases, such as ulcerative colitis. Thus, to assist the UC diagnosis, it is necessary to develop a new technology that can retrieve similar cases of diagnostic target image from cases in the past that stored the diagnosed images with various symptoms of colonic mucosa. In order to assist diagnoses with optical colonoscopy, this paper proposes a retrieval method for colonoscopy images that can cope with multiscale objects. The proposed method can retrieve similar colonoscopy images despite varying visible sizes of the target objects. Through three experiments conducted with real clinical colonoscopy images, we demonstrate that the method is able to retrieve objects of any visible size and any location at a high level of accuracy.

  16. Three-Dimensional Imaging by Self-Reference Single-Channel Digital Incoherent Holography

    PubMed Central

    Rosen, Joseph; Kelner, Roy

    2016-01-01

    Digital holography offers a reliable and fast method to image a three-dimensional scene from a single perspective. This article reviews recent developments of self-reference single-channel incoherent hologram recorders. Hologram recorders in which both interfering beams, commonly referred to as the signal and the reference beams, originate from the same observed objects are considered as self-reference systems. Moreover, the hologram recorders reviewed herein are configured in a setup of a single channel interferometer. This unique configuration is achieved through the use of one or more spatial light modulators. PMID:28757811

  17. Using Saliency-Weighted Disparity Statistics for Objective Visual Comfort Assessment of Stereoscopic Images

    NASA Astrophysics Data System (ADS)

    Zhang, Wenlan; Luo, Ting; Jiang, Gangyi; Jiang, Qiuping; Ying, Hongwei; Lu, Jing

    2016-06-01

    Visual comfort assessment (VCA) for stereoscopic images is a particularly significant yet challenging task in 3D quality of experience research field. Although the subjective assessment given by human observers is known as the most reliable way to evaluate the experienced visual discomfort, it is time-consuming and non-systematic. Therefore, it is of great importance to develop objective VCA approaches that can faithfully predict the degree of visual discomfort as human beings do. In this paper, a novel two-stage objective VCA framework is proposed. The main contribution of this study is that the important visual attention mechanism of human visual system is incorporated for visual comfort-aware feature extraction. Specifically, in the first stage, we first construct an adaptive 3D visual saliency detection model to derive saliency map of a stereoscopic image, and then a set of saliency-weighted disparity statistics are computed and combined to form a single feature vector to represent a stereoscopic image in terms of visual comfort. In the second stage, a high dimensional feature vector is fused into a single visual comfort score by performing random forest algorithm. Experimental results on two benchmark databases confirm the superior performance of the proposed approach.

  18. A Single-Phase Embedded Z-Source DC-AC Inverter

    PubMed Central

    Kim, Se-Jin; Lim, Young-Cheol

    2014-01-01

    In the conventional DC-AC inverter consisting of two DC-DC converters with unipolar output capacitors, the output capacitor voltages of the DC-DC converters must be higher than the DC input voltage. To overcome this weakness, this paper proposes a single-phase DC-AC inverter consisting of two embedded Z-source converters with bipolar output capacitors. The proposed inverter is composed of two embedded Z-source converters with a common DC source and output AC load. Though the output capacitor voltages of the converters are relatively low compared to those of a conventional inverter, an equivalent level of AC output voltages can be obtained. Moreover, by controlling the output capacitor voltages asymmetrically, the AC output voltage of the proposed inverter can be higher than the DC input voltage. To verify the validity of the proposed inverter, experiments were performed with a DC source voltage of 38 V. By controlling the output capacitor voltages of the converters symmetrically or asymmetrically, the proposed inverter can produce sinusoidal AC output voltages. The experiments show that efficiencies of up to 95% and 97% can be achieved with the proposed inverter using symmetric and asymmetric control, respectively. PMID:25133241

  19. A single-phase embedded Z-source DC-AC inverter.

    PubMed

    Kim, Se-Jin; Lim, Young-Cheol

    2014-01-01

    In the conventional DC-AC inverter consisting of two DC-DC converters with unipolar output capacitors, the output capacitor voltages of the DC-DC converters must be higher than the DC input voltage. To overcome this weakness, this paper proposes a single-phase DC-AC inverter consisting of two embedded Z-source converters with bipolar output capacitors. The proposed inverter is composed of two embedded Z-source converters with a common DC source and output AC load. Though the output capacitor voltages of the converters are relatively low compared to those of a conventional inverter, an equivalent level of AC output voltages can be obtained. Moreover, by controlling the output capacitor voltages asymmetrically, the AC output voltage of the proposed inverter can be higher than the DC input voltage. To verify the validity of the proposed inverter, experiments were performed with a DC source voltage of 38 V. By controlling the output capacitor voltages of the converters symmetrically or asymmetrically, the proposed inverter can produce sinusoidal AC output voltages. The experiments show that efficiencies of up to 95% and 97% can be achieved with the proposed inverter using symmetric and asymmetric control, respectively.

  20. A novel dual-color bifocal imaging system for single-molecule studies.

    PubMed

    Jiang, Chang; Kaul, Neha; Campbell, Jenna; Meyhofer, Edgar

    2017-05-01

    In this paper, we report the design and implementation of a dual-color bifocal imaging (DBI) system that is capable of acquiring two spectrally distinct, spatially registered images of objects located in either same or two distinct focal planes. We achieve this by separating an image into two channels with distinct chromatic properties and independently focusing both images onto a single CCD camera. The two channels in our device are registered with subpixel accuracy, and long-term stability of the registered images with nanometer-precision was accomplished by reducing the drift of the images to ∼5 nm. We demonstrate the capabilities of our DBI system by imaging biomolecules labeled with spectrally distinct dyes and micro- and nano-sized spheres located in different focal planes.

  1. Laser-induced acoustic imaging of underground objects

    NASA Astrophysics Data System (ADS)

    Li, Wen; DiMarzio, Charles A.; McKnight, Stephen W.; Sauermann, Gerhard O.; Miller, Eric L.

    1999-02-01

    This paper introduces a new demining technique based on the photo-acoustic interaction, together with results from photo- acoustic experiments. We have buried different types of targets (metal, rubber and plastic) in different media (sand, soil and water) and imaged them by measuring reflection of acoustic waves generated by irradiation with a CO2 laser. Research has been focused on the signal acquisition and signal processing. A deconvolution method using Wiener filters is utilized in data processing. Using a uniform spatial distribution of laser pulses at the ground's surface, we obtained 3D images of buried objects. The images give us a clear representation of the shapes of the underground objects. The quality of the images depends on the mismatch of acoustic impedance of the buried objects, the bandwidth and center frequency of the acoustic sensors and the selection of filter functions.

  2. Single-snapshot 2D color measurement by plenoptic imaging system

    NASA Astrophysics Data System (ADS)

    Masuda, Kensuke; Yamanaka, Yuji; Maruyama, Go; Nagai, Sho; Hirai, Hideaki; Meng, Lingfei; Tosic, Ivana

    2014-03-01

    Plenoptic cameras enable capture of directional light ray information, thus allowing applications such as digital refocusing, depth estimation, or multiband imaging. One of the most common plenoptic camera architectures contains a microlens array at the conventional image plane and a sensor at the back focal plane of the microlens array. We leverage the multiband imaging (MBI) function of this camera and develop a single-snapshot, single-sensor high color fidelity camera. Our camera is based on a plenoptic system with XYZ filters inserted in the pupil plane of the main lens. To achieve high color measurement precision of this system, we perform an end-to-end optimization of the system model that includes light source information, object information, optical system information, plenoptic image processing and color estimation processing. Optimized system characteristics are exploited to build an XYZ plenoptic colorimetric camera prototype that achieves high color measurement precision. We describe an application of our colorimetric camera to color shading evaluation of display and show that it achieves color accuracy of ΔE<0.01.

  3. Efficient single-pixel multispectral imaging via non-mechanical spatio-spectral modulation.

    PubMed

    Li, Ziwei; Suo, Jinli; Hu, Xuemei; Deng, Chao; Fan, Jingtao; Dai, Qionghai

    2017-01-27

    Combining spectral imaging with compressive sensing (CS) enables efficient data acquisition by fully utilizing the intrinsic redundancies in natural images. Current compressive multispectral imagers, which are mostly based on array sensors (e.g, CCD or CMOS), suffer from limited spectral range and relatively low photon efficiency. To address these issues, this paper reports a multispectral imaging scheme with a single-pixel detector. Inspired by the spatial resolution redundancy of current spatial light modulators (SLMs) relative to the target reconstruction, we design an all-optical spectral splitting device to spatially split the light emitted from the object into several counterparts with different spectrums. Separated spectral channels are spatially modulated simultaneously with individual codes by an SLM. This no-moving-part modulation ensures a stable and fast system, and the spatial multiplexing ensures an efficient acquisition. A proof-of-concept setup is built and validated for 8-channel multispectral imaging within 420~720 nm wavelength range on both macro and micro objects, showing a potential for efficient multispectral imager in macroscopic and biomedical applications.

  4. Hyperspectral imaging simulation of object under sea-sky background

    NASA Astrophysics Data System (ADS)

    Wang, Biao; Lin, Jia-xuan; Gao, Wei; Yue, Hui

    2016-10-01

    Remote sensing image simulation plays an important role in spaceborne/airborne load demonstration and algorithm development. Hyperspectral imaging is valuable in marine monitoring, search and rescue. On the demand of spectral imaging of objects under the complex sea scene, physics based simulation method of spectral image of object under sea scene is proposed. On the development of an imaging simulation model considering object, background, atmosphere conditions, sensor, it is able to examine the influence of wind speed, atmosphere conditions and other environment factors change on spectral image quality under complex sea scene. Firstly, the sea scattering model is established based on the Philips sea spectral model, the rough surface scattering theory and the water volume scattering characteristics. The measured bi directional reflectance distribution function (BRDF) data of objects is fit to the statistical model. MODTRAN software is used to obtain solar illumination on the sea, sky brightness, the atmosphere transmittance from sea to sensor and atmosphere backscattered radiance, and Monte Carlo ray tracing method is used to calculate the sea surface object composite scattering and spectral image. Finally, the object spectrum is acquired by the space transformation, radiation degradation and adding the noise. The model connects the spectrum image with the environmental parameters, the object parameters, and the sensor parameters, which provide a tool for the load demonstration and algorithm development.

  5. Influence of Objective Three-Dimensional Measures and Movement Images on Surgeon Treatment Planning for Lip Revision Surgery

    PubMed Central

    Trotman, Carroll-Ann; Phillips, Ceib; Faraway, Julian J.; Hartman, Terry; van Aalst, John A.

    2013-01-01

    Objective To determine whether a systematic evaluation of facial soft tissues of patients with cleft lip and palate, using facial video images and objective three-dimensional measurements of movement, change surgeons’ treatment plans for lip revision surgery. Design Prospective longitudinal study. Setting The University of North Carolina School of Dentistry. Patients, Participants A group of patients with repaired cleft lip and palate (n = 21), a noncleft control group (n = 37), and surgeons experienced in cleft care. Interventions Lip revision. Main Outcome Measures (1) facial photographic images; (2) facial video images during animations; (3) objective three-dimensional measurements of upper lip movement based on z scores; and (4) objective dynamic and visual three-dimensional measurement of facial soft tissue movement. Results With the use of the video images plus objective three-dimensional measures, changes were made to the problem list of the surgical treatment plan for 86% of the patients (95% confidence interval, 0.64 to 0.97) and the surgical goals for 71% of the patients (95% confidence interval, 0.48 to 0.89). The surgeon group varied in the percentage of patients for whom the problem list was modified, ranging from 24% (95% confidence interval, 8% to 47%) to 48% (95% confidence interval, 26% to 70%) of patients, and the percentage for whom the surgical goals were modified, ranging from 14% (94% confidence interval, 3% to 36%) to 48% (95% confidence interval, 26% to 70%) of patients. Conclusions For all surgeons, the additional assessment components of the systematic valuation resulted in a change in clinical decision making for some patients. PMID:23855676

  6. Cyclops: single-pixel imaging lidar system based on compressive sensing

    NASA Astrophysics Data System (ADS)

    Magalhães, F.; Correia, M. V.; Farahi, F.; Pereira do Carmo, J.; Araújo, F. M.

    2017-11-01

    Mars and the Moon are envisaged as major destinations of future space exploration missions in the upcoming decades. Imaging LIDARs are seen as a key enabling technology in the support of autonomous guidance, navigation and control operations, as they can provide very accurate, wide range, high-resolution distance measurements as required for the exploration missions. Imaging LIDARs can be used at critical stages of these exploration missions, such as descent and selection of safe landing sites, rendezvous and docking manoeuvres, or robotic surface navigation and exploration. Despite these devices have been commercially available and used for long in diverse metrology and ranging applications, their size, mass and power consumption are still far from being suitable and attractive for space exploratory missions. Here, we describe a compact Single-Pixel Imaging LIDAR System that is based on a compressive sensing technique. The application of the compressive codes to a DMD array enables compression of the spatial information, while the collection of timing histograms correlated to the pulsed laser source ensures image reconstruction at the ranged distances. Single-pixel cameras have been compared with raster scanning and array based counterparts in terms of noise performance, and proved to be superior. Since a single photodetector is used, a better SNR and higher reliability is expected in contrast with systems using large format photodetector arrays. Furthermore, the event of failure of one or more micromirror elements in the DMD does not prevent full reconstruction of the images. This brings additional robustness to the proposed 3D imaging LIDAR. The prototype that was implemented has three modes of operation. Range Finder: outputs the average distance between the system and the area of the target under illumination; Attitude Meter: provides the slope of the target surface based on distance measurements in three areas of the target; 3D Imager: produces 3D ranged

  7. Featured Image: Revealing Hidden Objects with Color

    NASA Astrophysics Data System (ADS)

    Kohler, Susanna

    2018-02-01

    Stunning color astronomical images can often be the motivation for astronomers to continue slogging through countless data files, calculations, and simulations as we seek to understand the mysteries of the universe. But sometimes the stunning images can, themselves, be the source of scientific discovery. This is the case with the below image of Lynds Dark Nebula 673, located in the Aquila constellation, that was captured with the Mayall 4-meter telescope at Kitt Peak National Observatory by a team of scientists led by Travis Rector (University of Alaska Anchorage). After creating the image with a novel color-composite imaging method that reveals faint H emission (visible in red in both images here), Rector and collaborators identified the presence of a dozen new Herbig-Haro objects small cloud patches that are caused when material is energetically flung out from newly born stars. The image adapted above shows three of the new objects, HH 118789, aligned with two previously known objects, HH 32 and 332 suggesting they are driven by the same source. For more beautiful images and insight into the authors discoveries, check out the article linked below!Full view of Lynds Dark Nebula 673. Click for the larger view this beautiful composite image deserves! [T.A. Rector (University of Alaska Anchorage) and H. Schweiker (WIYN and NOAO/AURA/NSF)]CitationT. A. Rector et al 2018 ApJ 852 13. doi:10.3847/1538-4357/aa9ce1

  8. Learning-based stochastic object models for use in optimizing imaging systems

    NASA Astrophysics Data System (ADS)

    Dolly, Steven R.; Anastasio, Mark A.; Yu, Lifeng; Li, Hua

    2017-03-01

    It is widely known that the optimization of imaging systems based on objective, or task-based, measures of image quality via computer-simulation requires use of a stochastic object model (SOM). However, the development of computationally tractable SOMs that can accurately model the statistical variations in anatomy within a specified ensemble of patients remains a challenging task. Because they are established by use of image data corresponding a single patient, previously reported numerical anatomical models lack of the ability to accurately model inter- patient variations in anatomy. In certain applications, however, databases of high-quality volumetric images are available that can facilitate this task. In this work, a novel and tractable methodology for learning a SOM from a set of volumetric training images is developed. The proposed method is based upon geometric attribute distribution (GAD) models, which characterize the inter-structural centroid variations and the intra-structural shape variations of each individual anatomical structure. The GAD models are scalable and deformable, and constrained by their respective principal attribute variations learned from training data. By use of the GAD models, random organ shapes and positions can be generated and integrated to form an anatomical phantom. The randomness in organ shape and position will reflect the variability of anatomy present in the training data. To demonstrate the methodology, a SOM corresponding to the pelvis of an adult male was computed and a corresponding ensemble of phantoms was created. Additionally, computer-simulated X-ray projection images corresponding to the phantoms were computed, from which tomographic images were reconstructed.

  9. Imaging of High-Z doped, Imploded Capsule Cores

    NASA Astrophysics Data System (ADS)

    Prisbrey, Shon T.; Edwards, M. John; Suter, Larry J.

    2006-10-01

    The ability to correctly ascertain the shape of imploded fusion capsules is critical to be able to achieve the spherical symmetry needed to maximize the energy yield of proposed fusion experiments for the National Ignition Facility. Implosion of the capsule creates a hot, dense core. The introduction of a high-Z dopant into the gas-filled core of the capsule increases the amount of bremsstrahlung radiation produced in the core and should make the imaging of the imploded core easier. Images of the imploded core can then be analyzed to ascertain the symmetry of the implosion. We calculate that the addition of Ne gas into a deuterium gas core will increase the amount of radiation emission while preserving the surrogacy of the radiation and hydrodynamics in the indirect drive NIF hohlraum in the proposed cryogenic hohlraums. The increased emission will more easily enable measurement of asymmetries and tuning of the implosion.

  10. Sci-Thur AM: YIS – 07: Optimizing dual-energy x-ray parameters using a single filter for both high and low-energy images to enhance soft-tissue imaging

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bowman, Wesley; Sattarivand, Mike

    Objective: To optimize dual-energy parameters of ExacTrac stereoscopic x-ray imaging system for lung SBRT patients Methods: Simulated spectra and a lung phantom were used to optimize filter material, thickness, kVps, and weighting factors to obtain bone subtracted dual-energy images. Spektr simulations were used to identify material in the atomic number (Z) range [3–83] based on a metric defined to separate spectrums of high and low energies. Both energies used the same filter due to time constraints of image acquisition in lung SBRT imaging. A lung phantom containing bone, soft tissue, and a tumor mimicking material was imaged with filter thicknessesmore » range [0–1] mm and kVp range [60–140]. A cost function based on contrast-to-noise-ratio of bone, soft tissue, and tumor, as well as image noise content, was defined to optimize filter thickness and kVp. Using the optimized parameters, dual-energy images of anthropomorphic Rando phantom were acquired and evaluated for bone subtraction. Imaging dose was measured with dual-energy technique using tin filtering. Results: Tin was the material of choice providing the best energy separation, non-toxicity, and non-reactiveness. The best soft-tissue-only image in the lung phantom was obtained using 0.3 mm tin and [140, 80] kVp pair. Dual-energy images of the Rando phantom had noticeable bone elimination when compared to no filtration. Dose was lower with tin filtering compared to no filtration. Conclusions: Dual-energy soft-tissue imaging is feasible using ExacTrac stereoscopic imaging system utilizing a single tin filter for both high and low energies and optimized acquisition parameters.« less

  11. Extremely Red Objects in Two Quasar Fields at z ~ 1.4

    NASA Astrophysics Data System (ADS)

    Stockton, Alan; McGrath, Elizabeth; Canalizo, Gabriela

    2006-10-01

    We present an investigation of the properties and environments of bright extremely red objects (EROs) found in the fields of the quasars TXS 0145+386 and 4C 15.55, both at z~1.4. There is marginal evidence from Chandra Advanced CCD Imaging Spectrometer (ACIS) imaging for hot cluster gas with a luminosity of a few 1044 ergs s-1 in the field of 4C 15.55. The TXS 0145+386 field has an upper limit at a similar value, but it also clearly shows an overdensity of faint galaxies. None of the EROs are detected as X-ray sources. For two of the EROs that have spectral energy distributions and rest-frame near-UV spectra that show that they are strongly dominated by old stellar populations, we determine radial surface brightness profiles from adaptive optics images. Both of these galaxies are best fit by profiles close to exponentials, plus a compact nucleus comprising ~30% of the total light in one case and 8% in the other. Neither is well fit by an r1/4-law profile. This apparent evidence for the formation of massive ~2×1011 disks of old stars in the early universe indicates that at least some galaxies formed essentially monolithically, with high star formation rates sustained over a few 108 yr and without the aid of major mergers. Based in part on data collected at the Subaru Telescope, which is operated by the National Astronomical Observatory of Japan; on data obtained with the Chandra X-Ray Observatory, which is operated for NASA by the Smithsonian Astrophysical Observatory; on data obtained at the Canada-France-Hawaii Telescope, which is operated by the National Research Council of Canada, the Institut National des Sciences de l'Univers of the Centre National de la Recherche Scientifique of France, and the University of Hawaii; and on data obtained at the the United Kingdom Infrared Telescope, which is operated by the Joint Astronomy Centre on behalf of the UK. Particle Physics and Astronomy Research Council. Some of the data presented herein were obtained at the W. M

  12. A programmable light engine for quantitative single molecule TIRF and HILO imaging.

    PubMed

    van 't Hoff, Marcel; de Sars, Vincent; Oheim, Martin

    2008-10-27

    We report on a simple yet powerful implementation of objective-type total internal reflection fluorescence (TIRF) and highly inclined and laminated optical sheet (HILO, a type of dark-field) illumination. Instead of focusing the illuminating laser beam to a single spot close to the edge of the microscope objective, we are scanning during the acquisition of a fluorescence image the focused spot in a circular orbit, thereby illuminating the sample from various directions. We measure parameters relevant for quantitative image analysis during fluorescence image acquisition by capturing an image of the excitation light distribution in an equivalent objective backfocal plane (BFP). Operating at scan rates above 1 MHz, our programmable light engine allows directional averaging by circular spinning the spot even for sub-millisecond exposure times. We show that restoring the symmetry of TIRF/HILO illumination reduces scattering and produces an evenly lit field-of-view that affords on-line analysis of evanescnt-field excited fluorescence without pre-processing. Utilizing crossed acousto-optical deflectors, our device generates arbitrary intensity profiles in BFP, permitting variable-angle, multi-color illumination, or objective lenses to be rapidly exchanged.

  13. Theory and applications of structured light single pixel imaging

    NASA Astrophysics Data System (ADS)

    Stokoe, Robert J.; Stockton, Patrick A.; Pezeshki, Ali; Bartels, Randy A.

    2018-02-01

    Many single-pixel imaging techniques have been developed in recent years. Though the methods of image acquisition vary considerably, the methods share unifying features that make general analysis possible. Furthermore, the methods developed thus far are based on intuitive processes that enable simple and physically-motivated reconstruction algorithms, however, this approach may not leverage the full potential of single-pixel imaging. We present a general theoretical framework of single-pixel imaging based on frame theory, which enables general, mathematically rigorous analysis. We apply our theoretical framework to existing single-pixel imaging techniques, as well as provide a foundation for developing more-advanced methods of image acquisition and reconstruction. The proposed frame theoretic framework for single-pixel imaging results in improved noise robustness, decrease in acquisition time, and can take advantage of special properties of the specimen under study. By building on this framework, new methods of imaging with a single element detector can be developed to realize the full potential associated with single-pixel imaging.

  14. RELICS: A Candidate Galaxy Arc at z~10 and Other Brightly Lensed z>6 Galaxies

    NASA Astrophysics Data System (ADS)

    Salmon, Brett; Coe, Dan; Bradley, Larry; Bradac, Marusa; Huang, Kuang-Han; Oesch, Pascal; Brammer, Gabriel; Stark, Daniel P.; Sharon, Keren; Trenti, Michele; Avila, Roberto J.; Ogaz, Sara; Acebron, Ana; Andrade-Santos, Felipe; Carrasco, Daniela; Cerny, Catherine; Cibirka, Nathália; Dawson, William; Frye, Brenda; Hoag, Austin; Jones, Christine; Mainali, Ramesh; Ouchi, Masami; Paterno-Mahler, Rachel; Rodney, Steven; Umetsu, Keiichi; Zitrin, Adi; RELICS

    2018-01-01

    Massive foreground galaxy clusters magnify and distort the light of objects behind them, permitting a view into both the extremely distant and intrinsically faint galaxy populations. We present here some of the most brightly lensed z>6 galaxy candidates known from the Reionization Lensing Cluster Survey (RELICS) and the discovery of a particularly fortuitous z~10 galaxy candidate which has been arced by the effects of strong gravitational lensing. The z~10 candidate has a lensed H-band magnitude of 25.8 AB mag and a high lensing magnification (~4-7). The inferred upper limits on the stellar mass (log [M_star /M_Sun]=9.5) and star formation rate (log [SFR/(M_Sun/yr)]=1.5) indicate that this candidate is a typical star-forming galaxy on the z>6 SFR-M_star relation. We rule out the only low-z solution as unphysical based on the required stellar mass, dust attenuation, size, and [OIII] EW needed for a z~2 SED to match the data. Finally, we reconstruct the source-plane image and estimate the candidate's physical size at z~10, finding a half-light radius of r_e < 0.8 kpc that is in line with the sizes of other z>9 candidates. While the James Webb Space Telescope will detect z>10 with ease, this rare candidate offers the potential for unprecedented spatial resolution less than 500 Myr after the Big Bang.

  15. Discovery of bright z ≃ 7 galaxies in the UltraVISTA survey

    NASA Astrophysics Data System (ADS)

    Bowler, R. A. A.; Dunlop, J. S.; McLure, R. J.; McCracken, H. J.; Milvang-Jensen, B.; Furusawa, H.; Fynbo, J. P. U.; Le Fèvre, O.; Holt, J.; Ideue, Y.; Ihara, Y.; Rogers, A. B.; Taniguchi, Y.

    2012-11-01

    We have exploited the new, deep, near-infrared UltraVISTA imaging of the Cosmological Evolution Survey (COSMOS) field, in tandem with deep optical and mid-infrared imaging, to conduct a new search for luminous galaxies at redshifts z ≃ 7. The year-one UltraVISTA data provide contiguous Y, J, H, Ks imaging over 1.5 deg2, reaching a 5σ detection limit of Y + J ≃ 25 (AB mag, 2-arcsec-diameter aperture). The central ≃1 deg2 of this imaging coincides with the final deep optical (u*, g, r, i) data provided by the Canada-France-Hawaii Telescope (CFHT) Legacy Survey and new deep Subaru/Suprime-Cam z'-band imaging obtained specifically to enable full exploitation of UltraVISTA. It also lies within the Hubble Space Telescope (HST) I814 band and Spitzer/Infrared Array Camera imaging obtained as part of the COSMOS survey. We have utilized this unique multiwavelength dataset to select galaxy candidates at redshifts z > 6.5 by searching first for Y + J-detected objects which are undetected in the CFHT and HST optical data. This sample was then refined using a photometric redshift fitting code, enabling the rejection of lower redshift galaxy contaminants and cool galactic M, L, T dwarf stars. The final result of this process is a small sample of (at most) 10 credible galaxy candidates at z > 6.5 (from over 200 000 galaxies detected in the year-one UltraVISTA data) which we present in this paper. The first four of these appear to be robust galaxies at z > 6.5, and fitting to their stacked spectral energy distribution yields zphot = 6.98 ± 0.05 with a stellar mass M* ≃ 5 × 109 M⊙ and rest-frame ultraviolet (UV) spectral slope β ≃ -2.0 ± 0.2 (where fλ ∝ λβ). The next three are also good candidates for z > 6.5 galaxies, but the possibility that they are dwarf stars cannot be completely excluded. Our final subset of three additional candidates is afflicted not only by potential dwarf star contamination, but also contains objects likely to lie at redshifts just

  16. There's Waldo! A Normalization Model of Visual Search Predicts Single-Trial Human Fixations in an Object Search Task

    PubMed Central

    Miconi, Thomas; Groomes, Laura; Kreiman, Gabriel

    2016-01-01

    When searching for an object in a scene, how does the brain decide where to look next? Visual search theories suggest the existence of a global “priority map” that integrates bottom-up visual information with top-down, target-specific signals. We propose a mechanistic model of visual search that is consistent with recent neurophysiological evidence, can localize targets in cluttered images, and predicts single-trial behavior in a search task. This model posits that a high-level retinotopic area selective for shape features receives global, target-specific modulation and implements local normalization through divisive inhibition. The normalization step is critical to prevent highly salient bottom-up features from monopolizing attention. The resulting activity pattern constitues a priority map that tracks the correlation between local input and target features. The maximum of this priority map is selected as the locus of attention. The visual input is then spatially enhanced around the selected location, allowing object-selective visual areas to determine whether the target is present at this location. This model can localize objects both in array images and when objects are pasted in natural scenes. The model can also predict single-trial human fixations, including those in error and target-absent trials, in a search task involving complex objects. PMID:26092221

  17. The impact of low-Z and high-Z metal implants in IMRT: A Monte Carlo study of dose inaccuracies in commercial dose algorithms

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Spadea, Maria Francesca, E-mail: mfspadea@unicz.it; Verburg, Joost Mathias; Seco, Joao

    2014-01-15

    Purpose: The aim of the study was to evaluate the dosimetric impact of low-Z and high-Z metallic implants on IMRT plans. Methods: Computed tomography (CT) scans of three patients were analyzed to study effects due to the presence of Titanium (low-Z), Platinum and Gold (high-Z) inserts. To eliminate artifacts in CT images, a sinogram-based metal artifact reduction algorithm was applied. IMRT dose calculations were performed on both the uncorrected and corrected images using a commercial planning system (convolution/superposition algorithm) and an in-house Monte Carlo platform. Dose differences between uncorrected and corrected datasets were computed and analyzed using gamma index (Pγ{submore » <1}) and setting 2 mm and 2% as distance to agreement and dose difference criteria, respectively. Beam specific depth dose profiles across the metal were also examined. Results: Dose discrepancies between corrected and uncorrected datasets were not significant for low-Z material. High-Z materials caused under-dosage of 20%–25% in the region surrounding the metal and over dosage of 10%–15% downstream of the hardware. Gamma index test yielded Pγ{sub <1}>99% for all low-Z cases; while for high-Z cases it returned 91% < Pγ{sub <1}< 99%. Analysis of the depth dose curve of a single beam for low-Z cases revealed that, although the dose attenuation is altered inside the metal, it does not differ downstream of the insert. However, for high-Z metal implants the dose is increased up to 10%–12% around the insert. In addition, Monte Carlo method was more sensitive to the presence of metal inserts than superposition/convolution algorithm. Conclusions: The reduction in terms of dose of metal artifacts in CT images is relevant for high-Z implants. In this case, dose distribution should be calculated using Monte Carlo algorithms, given their superior accuracy in dose modeling in and around the metal. In addition, the knowledge of the composition of metal inserts improves the

  18. Stripe nonuniformity correction for infrared imaging system based on single image optimization

    NASA Astrophysics Data System (ADS)

    Hua, Weiping; Zhao, Jufeng; Cui, Guangmang; Gong, Xiaoli; Ge, Peng; Zhang, Jiang; Xu, Zhihai

    2018-06-01

    Infrared imaging is often disturbed by stripe nonuniformity noise. Scene-based correction method can effectively reduce the impact of stripe noise. In this paper, a stripe nonuniformity correction method based on differential constraint is proposed. Firstly, the gray distribution of stripe nonuniformity is analyzed and the penalty function is constructed by the difference of horizontal gradient and vertical gradient. With the weight function, the penalty function is optimized to obtain the corrected image. Comparing with other single-frame approaches, experiments show that the proposed method performs better in both subjective and objective analysis, and does less damage to edge and detail. Meanwhile, the proposed method runs faster. We have also discussed the differences between the proposed idea and multi-frame methods. Our method is finally well applied in hardware system.

  19. Characteristics of extreme ultraviolet emission from high-Z plasmas

    NASA Astrophysics Data System (ADS)

    Ohashi, H.; Higashiguchi, T.; Suzuki, Y.; Kawasaki, M.; Suzuki, C.; Tomita, K.; Nishikino, M.; Fujioka, S.; Endo, A.; Li, B.; Otsuka, T.; Dunne, P.; O'Sullivan, G.

    2016-03-01

    We demonstrate the extreme ultraviolet (EUV) and soft x-ray sources in the 2 to 7 nm spectral region related to the beyond EUV (BEUV) question at 6.x nm and the water window source based on laser-produced high-Z plasmas. Resonance emission from multiply charged ions merges to produce intense unresolved transition arrays (UTAs), extending below the carbon K edge (4.37 nm). An outline of a microscope design for single-shot live cell imaging is proposed based on high-Z plasma UTA source, coupled to multilayer mirror optics.

  20. Objective quality assessment for multiexposure multifocus image fusion.

    PubMed

    Hassen, Rania; Wang, Zhou; Salama, Magdy M A

    2015-09-01

    There has been a growing interest in image fusion technologies, but how to objectively evaluate the quality of fused images has not been fully understood. Here, we propose a method for objective quality assessment of multiexposure multifocus image fusion based on the evaluation of three key factors of fused image quality: 1) contrast preservation; 2) sharpness; and 3) structure preservation. Subjective experiments are conducted to create an image fusion database, based on which, performance evaluation shows that the proposed fusion quality index correlates well with subjective scores, and gives a significant improvement over the existing fusion quality measures.

  1. Automated imaging system for single molecules

    DOEpatents

    Schwartz, David Charles; Runnheim, Rodney; Forrest, Daniel

    2012-09-18

    There is provided a high throughput automated single molecule image collection and processing system that requires minimal initial user input. The unique features embodied in the present disclosure allow automated collection and initial processing of optical images of single molecules and their assemblies. Correct focus may be automatically maintained while images are collected. Uneven illumination in fluorescence microscopy is accounted for, and an overall robust imaging operation is provided yielding individual images prepared for further processing in external systems. Embodiments described herein are useful in studies of any macromolecules such as DNA, RNA, peptides and proteins. The automated image collection and processing system and method of same may be implemented and deployed over a computer network, and may be ergonomically optimized to facilitate user interaction.

  2. Multiple-3D-object secure information system based on phase shifting method and single interference.

    PubMed

    Li, Wei-Na; Shi, Chen-Xiao; Piao, Mei-Lan; Kim, Nam

    2016-05-20

    We propose a multiple-3D-object secure information system for encrypting multiple three-dimensional (3D) objects based on the three-step phase shifting method. During the decryption procedure, five phase functions (PFs) are decreased to three PFs, in comparison with our previous method, which implies that one cross beam splitter is utilized to implement the single decryption interference. Moreover, the advantages of the proposed scheme also include: each 3D object can be decrypted discretionarily without decrypting a series of other objects earlier; the quality of the decrypted slice image of each object is high according to the correlation coefficient values, none of which is lower than 0.95; no iterative algorithm is involved. The feasibility of the proposed scheme is demonstrated by computer simulation results.

  3. Single-camera stereo-digital image correlation with a four-mirror adapter: optimized design and validation

    NASA Astrophysics Data System (ADS)

    Yu, Liping; Pan, Bing

    2016-12-01

    A low-cost, easy-to-implement but practical single-camera stereo-digital image correlation (DIC) system using a four-mirror adapter is established for accurate shape and three-dimensional (3D) deformation measurements. The mirrors assisted pseudo-stereo imaging system can convert a single camera into two virtual cameras, which view a specimen from different angles and record the surface images of the test object onto two halves of the camera sensor. To enable deformation measurement in non-laboratory conditions or extreme high temperature environments, an active imaging optical design, combining an actively illuminated monochromatic source with a coupled band-pass optical filter, is compactly integrated to the pseudo-stereo DIC system. The optical design, basic principles and implementation procedures of the established system for 3D profile and deformation measurements are described in detail. The effectiveness and accuracy of the established system are verified by measuring the profile of a regular cylinder surface and displacements of a translated planar plate. As an application example, the established system is used to determine the tensile strains and Poisson's ratio of a composite solid propellant specimen during stress relaxation test. Since the established single-camera stereo-DIC system only needs a single camera and presents strong robustness against variations in ambient light or the thermal radiation of a hot object, it demonstrates great potential in determining transient deformation in non-laboratory or high-temperature environments with the aid of a single high-speed camera.

  4. Image BOSS: a biomedical object storage system

    NASA Astrophysics Data System (ADS)

    Stacy, Mahlon C.; Augustine, Kurt E.; Robb, Richard A.

    1997-05-01

    Researchers using biomedical images have data management needs which are oriented perpendicular to clinical PACS. The image BOSS system is designed to permit researchers to organize and select images based on research topic, image metadata, and a thumbnail of the image. Image information is captured from existing images in a Unix based filesystem, stored in an object oriented database, and presented to the user in a familiar laboratory notebook metaphor. In addition, the ImageBOSS is designed to provide an extensible infrastructure for future content-based queries directly on the images.

  5. A multi-resolution strategy for a multi-objective deformable image registration framework that accommodates large anatomical differences

    NASA Astrophysics Data System (ADS)

    Alderliesten, Tanja; Bosman, Peter A. N.; Sonke, Jan-Jakob; Bel, Arjan

    2014-03-01

    Currently, two major challenges dominate the field of deformable image registration. The first challenge is related to the tuning of the developed methods to specific problems (i.e. how to best combine different objectives such as similarity measure and transformation effort). This is one of the reasons why, despite significant progress, clinical implementation of such techniques has proven to be difficult. The second challenge is to account for large anatomical differences (e.g. large deformations, (dis)appearing structures) that occurred between image acquisitions. In this paper, we study a framework based on multi-objective optimization to improve registration robustness and to simplify tuning for specific applications. Within this framework we specifically consider the use of an advanced model-based evolutionary algorithm for optimization and a dual-dynamic transformation model (i.e. two "non-fixed" grids: one for the source- and one for the target image) to accommodate for large anatomical differences. The framework computes and presents multiple outcomes that represent efficient trade-offs between the different objectives (a so-called Pareto front). In image processing it is common practice, for reasons of robustness and accuracy, to use a multi-resolution strategy. This is, however, only well-established for single-objective registration methods. Here we describe how such a strategy can be realized for our multi-objective approach and compare its results with a single-resolution strategy. For this study we selected the case of prone-supine breast MRI registration. Results show that the well-known advantages of a multi-resolution strategy are successfully transferred to our multi-objective approach, resulting in superior (i.e. Pareto-dominating) outcomes.

  6. HST images of very compact blue galaxies at z approximately 0.2

    NASA Technical Reports Server (NTRS)

    Koo, David C.; Bershady, Matthew A.; Wirth, Gregory D.; Stanford, S. Adam; Majewski, Steven R.

    1994-01-01

    We present the results of Hubble Space Telescope (HST) Wide-Field Camera (WFC) imaging of seven very compact, very blue galaxies with B less than or equal to 21 and redshifts z approximately 0.1 to 0.35. Based on deconvolved images, we estimate typical half-light diameters of approximately 0.65 sec, corresponding to approximately 1.4 h(exp -1) kpc at redshifts z approximately 0.2. The average rest frame surface brightness within this diameter is mu(sub v) approximately 20.5 mag arcsec(exp -2), approximately 1 mag brighter than that of typical late-type blue galaxies. Ground-based spectra show strong, narrow emission lines indicating high ionization; their very blue colors suggest recent bursts of star-formation; their typical luminosities are approximately 4 times fainter than that of field galaxies. These characteristics suggest H II galaxies as likely local counterparts of our sample, though our most luminous targets appear to be unusually compact for their luminosities.

  7. Single-shot spiral imaging at 7 T.

    PubMed

    Engel, Maria; Kasper, Lars; Barmet, Christoph; Schmid, Thomas; Vionnet, Laetitia; Wilm, Bertram; Pruessmann, Klaas P

    2018-03-25

    The purpose of this work is to explore the feasibility and performance of single-shot spiral MRI at 7 T, using an expanded signal model for reconstruction. Gradient-echo brain imaging is performed on a 7 T system using high-resolution single-shot spiral readouts and half-shot spirals that perform dual-image acquisition after a single excitation. Image reconstruction is based on an expanded signal model including the encoding effects of coil sensitivity, static off-resonance, and magnetic field dynamics. The latter are recorded concurrently with image acquisition, using NMR field probes. The resulting image resolution is assessed by point spread function analysis. Single-shot spiral imaging is achieved at a nominal resolution of 0.8 mm, using spiral-out readouts of 53-ms duration. High depiction fidelity is achieved without conspicuous blurring or distortion. Effective resolutions are assessed as 0.8, 0.94, and 0.98 mm in CSF, gray matter and white matter, respectively. High image quality is also achieved with half-shot acquisition yielding image pairs at 1.5-mm resolution. Use of an expanded signal model enables single-shot spiral imaging at 7 T with unprecedented image quality. Single-shot and half-shot spiral readouts deploy the sensitivity benefit of high field for rapid high-resolution imaging, particularly for functional MRI and arterial spin labeling. © 2018 International Society for Magnetic Resonance in Medicine.

  8. A single-pixel X-ray imager concept and its application to secure radiographic inspections

    DOE PAGES

    Gilbert, Andrew J.; Miller, Brian W.; Robinson, Sean M.; ...

    2017-07-01

    Imaging technology is generally considered too invasive for arms control inspections due to the concern that it cannot properly secure sensitive features of the inspected item. But, this same sensitive information, which could include direct information on the form and function of the items under inspection, could be used for robust arms control inspections. The single-pixel X-ray imager (SPXI) is introduced as a method to make such inspections, capturing the salient spatial information of an object in a secure manner while never forming an actual image. We built this method on the theory of compressive sensing and the single pixelmore » optical camera. The performance of the system is quantified using simulated inspections of simple objects. Measures of the robustness and security of the method are introduced and used to determine how robust and secure such an inspection would be. Particularly, it is found that an inspection with low noise (<1%) and high undersampling (>256×) exhibits high robustness and security.« less

  9. A single-pixel X-ray imager concept and its application to secure radiographic inspections

    NASA Astrophysics Data System (ADS)

    Gilbert, Andrew J.; Miller, Brian W.; Robinson, Sean M.; White, Timothy A.; Pitts, William Karl; Jarman, Kenneth D.; Seifert, Allen

    2017-07-01

    Imaging technology is generally considered too invasive for arms control inspections due to the concern that it cannot properly secure sensitive features of the inspected item. However, this same sensitive information, which could include direct information on the form and function of the items under inspection, could be used for robust arms control inspections. The single-pixel X-ray imager (SPXI) is introduced as a method to make such inspections, capturing the salient spatial information of an object in a secure manner while never forming an actual image. The method is built on the theory of compressive sensing and the single pixel optical camera. The performance of the system is quantified using simulated inspections of simple objects. Measures of the robustness and security of the method are introduced and used to determine how robust and secure such an inspection would be. In particular, it is found that an inspection with low noise ( < 1 %) and high undersampling ( > 256 ×) exhibits high robustness and security.

  10. Research into a Single-aperture Light Field Camera System to Obtain Passive Ground-based 3D Imagery of LEO Objects

    NASA Astrophysics Data System (ADS)

    Bechis, K.; Pitruzzello, A.

    2014-09-01

    This presentation describes our ongoing research into using a ground-based light field camera to obtain passive, single-aperture 3D imagery of LEO objects. Light field cameras are an emerging and rapidly evolving technology for passive 3D imaging with a single optical sensor. The cameras use an array of lenslets placed in front of the camera focal plane, which provides angle of arrival information for light rays originating from across the target, allowing range to target and 3D image to be obtained from a single image using monocular optics. The technology, which has been commercially available for less than four years, has the potential to replace dual-sensor systems such as stereo cameras, dual radar-optical systems, and optical-LIDAR fused systems, thus reducing size, weight, cost, and complexity. We have developed a prototype system for passive ranging and 3D imaging using a commercial light field camera and custom light field image processing algorithms. Our light field camera system has been demonstrated for ground-target surveillance and threat detection applications, and this paper presents results of our research thus far into applying this technology to the 3D imaging of LEO objects. The prototype 3D imaging camera system developed by Northrop Grumman uses a Raytrix R5 C2GigE light field camera connected to a Windows computer with an nVidia graphics processing unit (GPU). The system has a frame rate of 30 Hz, and a software control interface allows for automated camera triggering and light field image acquisition to disk. Custom image processing software then performs the following steps: (1) image refocusing, (2) change detection, (3) range finding, and (4) 3D reconstruction. In Step (1), a series of 2D images are generated from each light field image; the 2D images can be refocused at up to 100 different depths. Currently, steps (1) through (3) are automated, while step (4) requires some user interaction. A key requirement for light field camera

  11. Competition between B-Z and B-L transitions in a single DNA molecule: Computational studies

    NASA Astrophysics Data System (ADS)

    Kwon, Ah-Young; Nam, Gi-Moon; Johner, Albert; Kim, Seyong; Hong, Seok-Cheol; Lee, Nam-Kyung

    2016-02-01

    Under negative torsion, DNA adopts left-handed helical forms, such as Z-DNA and L-DNA. Using the random copolymer model developed for a wormlike chain, we represent a single DNA molecule with structural heterogeneity as a helical chain consisting of monomers which can be characterized by different helical senses and pitches. By Monte Carlo simulation, where we take into account bending and twist fluctuations explicitly, we study sequence dependence of B-Z transitions under torsional stress and tension focusing on the interaction with B-L transitions. We consider core sequences, (GC) n repeats or (TG) n repeats, which can interconvert between the right-handed B form and the left-handed Z form, imbedded in a random sequence, which can convert to left-handed L form with different (tension dependent) helical pitch. We show that Z-DNA formation from the (GC) n sequence is always supported by unwinding torsional stress but Z-DNA formation from the (TG) n sequence, which are more costly to convert but numerous, can be strongly influenced by the quenched disorder in the surrounding random sequence.

  12. Spectroscopy of Luminous z > 7 Galaxy Candidates and Sources of Contamination in z > 7 Galaxy Searches

    NASA Astrophysics Data System (ADS)

    Capak, P.; Mobasher, B.; Scoville, N. Z.; McCracken, H.; Ilbert, O.; Salvato, M.; Menéndez-Delmestre, K.; Aussel, H.; Carilli, C.; Civano, F.; Elvis, M.; Giavalisco, M.; Jullo, E.; Kartaltepe, J.; Leauthaud, A.; Koekemoer, A. M.; Kneib, J.-P.; LeFloch, E.; Sanders, D. B.; Schinnerer, E.; Shioya, Y.; Shopbell, P.; Tanaguchi, Y.; Thompson, D.; Willott, C. J.

    2011-04-01

    We present three bright z +-dropout candidates selected from deep near-infrared (NIR) imaging of the COSMOS 2 deg2 field. All three objects match the 0.8-8 μm colors of other published z > 7 candidates but are 3 mag brighter, facilitating further study. Deep spectroscopy of two of the candidates covering 0.64-1.02 μm with Keck-DEIMOS and all three covering 0.94-1.10 μm and 1.52-1.80 μm with Keck-NIRSPEC detects weak spectral features tentatively identified as Lyα at z = 6.95 and z = 7.69 in two of the objects. The third object is placed at z ~ 1.6 based on a 24 μm and weak optical detection. A comparison with the spectral energy distributions of known z < 7 galaxies, including objects with strong spectral lines, large extinction, and large systematic uncertainties in the photometry, yields no objects with similar colors. However, the λ > 1 μm properties of all three objects can be matched to optically detected sources with photometric redshifts at z ~ 1.8, so the non-detection in the i + and z + bands is the primary factor which favors a z > 7 solution. If any of these objects are at z ~ 7, the bright end of the luminosity function is significantly higher at z > 7 than suggested by previous studies, but consistent within the statistical uncertainty and the dark matter halo distribution. If these objects are at low redshift, the Lyman break selection must be contaminated by a previously unknown population of low-redshift objects with very strong breaks in their broadband spectral energy distributions and blue NIR colors. The implications of this result on luminosity function evolution at high redshift are discussed. We show that the primary limitation of z > 7 galaxy searches with broad filters is the depth of the available optical data. Based on observations with the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and the National Aeronautics and Space

  13. Laser radiography forming bremsstrahlung radiation to image an object

    DOEpatents

    Perry, Michael D.; Sefcik, Joseph A.

    2004-01-13

    A method of imaging an object by generating laser pulses with a short-pulse, high-power laser. When the laser pulse strikes a conductive target, bremsstrahlung radiation is generated such that hard ballistic high-energy electrons are formed to penetrate an object. A detector on the opposite side of the object detects these electrons. Since laser pulses are used to form the hard x-rays, multiple pulses can be used to image an object in motion, such as an exploding or compressing object, by using time gated detectors. Furthermore, the laser pulses can be directed down different tubes using mirrors and filters so that each laser pulse will image a different portion of the object.

  14. An Integrative Object-Based Image Analysis Workflow for Uav Images

    NASA Astrophysics Data System (ADS)

    Yu, Huai; Yan, Tianheng; Yang, Wen; Zheng, Hong

    2016-06-01

    In this work, we propose an integrative framework to process UAV images. The overall process can be viewed as a pipeline consisting of the geometric and radiometric corrections, subsequent panoramic mosaicking and hierarchical image segmentation for later Object Based Image Analysis (OBIA). More precisely, we first introduce an efficient image stitching algorithm after the geometric calibration and radiometric correction, which employs a fast feature extraction and matching by combining the local difference binary descriptor and the local sensitive hashing. We then use a Binary Partition Tree (BPT) representation for the large mosaicked panoramic image, which starts by the definition of an initial partition obtained by an over-segmentation algorithm, i.e., the simple linear iterative clustering (SLIC). Finally, we build an object-based hierarchical structure by fully considering the spectral and spatial information of the super-pixels and their topological relationships. Moreover, an optimal segmentation is obtained by filtering the complex hierarchies into simpler ones according to some criterions, such as the uniform homogeneity and semantic consistency. Experimental results on processing the post-seismic UAV images of the 2013 Ya'an earthquake demonstrate the effectiveness and efficiency of our proposed method.

  15. Method for detecting a mass density image of an object

    DOEpatents

    Wernick, Miles N [Chicago, IL; Yang, Yongyi [Westmont, IL

    2008-12-23

    A method for detecting a mass density image of an object. An x-ray beam is transmitted through the object and a transmitted beam is emitted from the object. The transmitted beam is directed at an angle of incidence upon a crystal analyzer. A diffracted beam is emitted from the crystal analyzer onto a detector and digitized. A first image of the object is detected from the diffracted beam emitted from the crystal analyzer when positioned at a first angular position. A second image of the object is detected from the diffracted beam emitted from the crystal analyzer when positioned at a second angular position. A refraction image is obtained and a regularized mathematical inversion algorithm is applied to the refraction image to obtain a mass density image.

  16. Determination of localization accuracy based on experimentally acquired image sets: applications to single molecule microscopy

    PubMed Central

    Tahmasbi, Amir; Ward, E. Sally; Ober, Raimund J.

    2015-01-01

    Fluorescence microscopy is a photon-limited imaging modality that allows the study of subcellular objects and processes with high specificity. The best possible accuracy (standard deviation) with which an object of interest can be localized when imaged using a fluorescence microscope is typically calculated using the Cramér-Rao lower bound, that is, the inverse of the Fisher information. However, the current approach for the calculation of the best possible localization accuracy relies on an analytical expression for the image of the object. This can pose practical challenges since it is often difficult to find appropriate analytical models for the images of general objects. In this study, we instead develop an approach that directly uses an experimentally collected image set to calculate the best possible localization accuracy for a general subcellular object. In this approach, we fit splines, i.e. smoothly connected piecewise polynomials, to the experimentally collected image set to provide a continuous model of the object, which can then be used for the calculation of the best possible localization accuracy. Due to its practical importance, we investigate in detail the application of the proposed approach in single molecule fluorescence microscopy. In this case, the object of interest is a point source and, therefore, the acquired image set pertains to an experimental point spread function. PMID:25837101

  17. Automatic single-image-based rain streaks removal via image decomposition.

    PubMed

    Kang, Li-Wei; Lin, Chia-Wen; Fu, Yu-Hsiang

    2012-04-01

    Rain removal from a video is a challenging problem and has been recently investigated extensively. Nevertheless, the problem of rain removal from a single image was rarely studied in the literature, where no temporal information among successive images can be exploited, making the problem very challenging. In this paper, we propose a single-image-based rain removal framework via properly formulating rain removal as an image decomposition problem based on morphological component analysis. Instead of directly applying a conventional image decomposition technique, the proposed method first decomposes an image into the low- and high-frequency (HF) parts using a bilateral filter. The HF part is then decomposed into a "rain component" and a "nonrain component" by performing dictionary learning and sparse coding. As a result, the rain component can be successfully removed from the image while preserving most original image details. Experimental results demonstrate the efficacy of the proposed algorithm.

  18. Label-free, single-object sensing with a microring resonator: FDTD simulation.

    PubMed

    Nguyen, Dan T; Norwood, Robert A

    2013-01-14

    Label-free, single-object sensing with a microring resonator is investigated numerically using the finite difference time-domain (FDTD) method. A pulse with ultra-wide bandwidth that spans over several resonant modes of the ring and of the sensing object is used for simulation, enabling a single-shot simulation of the microring sensing. The FDTD simulation not only can describe the circulation of the light in a whispering-gallery-mode (WGM) microring and multiple interactions between the light and the sensing object, but also other important factors of the sensing system, such as scattering and radiation losses. The FDTD results show that the simulation can yield a resonant shift of the WGM cavity modes. Furthermore, it can also extract eigenmodes of the sensing object, and therefore information from deep inside the object. The simulation method is not only suitable for a single object (single molecule, nano-, micro-scale particle) but can be extended to the problem of multiple objects as well.

  19. Single-particle investigation of summertime and wintertime Antarctic sea spray aerosols using low-Z particle EPMA, Raman microspectrometry, and ATR-FTIR imaging techniques

    NASA Astrophysics Data System (ADS)

    Eom, Hyo-Jin; Gupta, Dhrubajyoti; Cho, Hye-Rin; Hwang, Hee Jin; Do Hur, Soon; Gim, Yeontae; Ro, Chul-Un

    2016-11-01

    Two aerosol samples collected at King Sejong Korean scientific research station, Antarctica, on 9 December 2011 in the austral summer (sample S1) and 23 July 2012 in the austral winter (sample S2), when the oceanic chlorophyll a levels on the collection days of the samples were quite different, by ˜ 19 times (2.46 vs. 0.13 µg L-1, respectively), were investigated on a single-particle basis using quantitative energy-dispersive electron probe X-ray microanalysis (ED-EPMA), called low-Z particle EPMA, Raman microspectrometry (RMS), and attenuated total reflection Fourier transform infrared (ATR-FTIR) imaging techniques to obtain their characteristics based on the elemental chemical compositions, molecular species, and mixing state. X-ray analysis showed that the supermicron summertime and wintertime Antarctic aerosol samples have different elemental chemical compositions, even though all the individual particles analyzed were sea spray aerosols (SSAs); i.e., the contents of C, O, Ca, S, and Si were more elevated, whereas Cl was more depleted, for sample S1 than for sample S2. Based on qualitative analysis of the chemical species present in individual SSAs by the combined application of RMS and ATR-FTIR imaging, different organic species were observed in samples S1 and S2; i.e., Mg hydrate salts of alanine were predominant in samples S1 and S2, whereas Mg salts of fatty acids internally mixed with Mg hydrate salts of alanine were significant in sample S2. Although CaSO4 was observed significantly in both samples S1 and S2, other inorganic species, such as Na2SO4, NaNO3, Mg(NO3)2, SiO2, and CH3SO3Mg, were observed more significantly in sample S1, suggesting that those compounds may be related to the higher phytoplankton activity in summer.

  20. 220GHz wideband 3D imaging radar for concealed object detection technology development and phenomenology studies

    NASA Astrophysics Data System (ADS)

    Robertson, Duncan A.; Macfarlane, David G.; Bryllert, Tomas

    2016-05-01

    We present a 220 GHz 3D imaging `Pathfinder' radar developed within the EU FP7 project CONSORTIS (Concealed Object Stand-Off Real-Time Imaging for Security) which has been built to address two objectives: (i) to de-risk the radar hardware development and (ii) to enable the collection of phenomenology data with ~1 cm3 volumetric resolution. The radar combines a DDS-based chirp generator and self-mixing multiplier technology to achieve a 30 GHz bandwidth chirp with such high linearity that the raw point response is close to ideal and only requires minor nonlinearity compensation. The single transceiver is focused with a 30 cm lens mounted on a gimbal to acquire 3D volumetric images of static test targets and materials.

  1. Estimation of Image Sensor Fill Factor Using a Single Arbitrary Image

    PubMed Central

    Wen, Wei; Khatibi, Siamak

    2017-01-01

    Achieving a high fill factor is a bottleneck problem for capturing high-quality images. There are hardware and software solutions to overcome this problem. In the solutions, the fill factor is known. However, this is an industrial secrecy by most image sensor manufacturers due to its direct effect on the assessment of the sensor quality. In this paper, we propose a method to estimate the fill factor of a camera sensor from an arbitrary single image. The virtual response function of the imaging process and sensor irradiance are estimated from the generation of virtual images. Then the global intensity values of the virtual images are obtained, which are the result of fusing the virtual images into a single, high dynamic range radiance map. A non-linear function is inferred from the original and global intensity values of the virtual images. The fill factor is estimated by the conditional minimum of the inferred function. The method is verified using images of two datasets. The results show that our method estimates the fill factor correctly with significant stability and accuracy from one single arbitrary image according to the low standard deviation of the estimated fill factors from each of images and for each camera. PMID:28335459

  2. ARCADIA: a system for the integration of angiocardiographic data and images by an object-oriented DBMS.

    PubMed

    Pinciroli, F; Combi, C; Pozzi, G

    1995-02-01

    Use of data base techniques to store medical records has been going on for more than 40 years. Some aspects still remain unresolved, e.g., the management of textual data and image data within a single system. Object-orientation techniques applied to a database management system (DBMS) allow the definition of suitable data structures (e.g., to store digital images): some facilities allow the use of predefined structures when defining new ones. Currently available object-oriented DBMS, however, still need improvements both in the schema update and in the query facilities. This paper describes a prototype of a medical record that includes some multimedia features, managing both textual and image data. The prototype here described considers data from the medical records of patients subjected to percutaneous transluminal coronary artery angioplasty. We developed it on a Sun workstation with a Unix operating system and ONTOS as an object-oriented DBMS.

  3. Colloidal lenses allow high-temperature single-molecule imaging and improve fluorophore photostability

    NASA Astrophysics Data System (ADS)

    Schwartz, Jerrod J.; Stavrakis, Stavros; Quake, Stephen R.

    2010-02-01

    Although single-molecule fluorescence spectroscopy was first demonstrated at near-absolute zero temperatures (1.8 K), the field has since advanced to include room-temperature observations, largely owing to the use of objective lenses with high numerical aperture, brighter fluorophores and more sensitive detectors. This has opened the door for many chemical and biological systems to be studied at native temperatures at the single-molecule level both in vitro and in vivo. However, it is difficult to study systems and phenomena at temperatures above 37 °C, because the index-matching fluids used with high-numerical-aperture objective lenses can conduct heat from the sample to the lens, and sustained exposure to high temperatures can cause the lens to fail. Here, we report that TiO2 colloids with diameters of 2 µm and a high refractive index can act as lenses that are capable of single-molecule imaging at 70 °C when placed in immediate proximity to an emitting molecule. The optical system is completed by a low-numerical-aperture optic that can have a long working distance and an air interface, which allows the sample to be independently heated. Colloidal lenses were used for parallel imaging of surface-immobilized single fluorophores and for real-time single-molecule measurements of mesophilic and thermophilic enzymes at 70 °C. Fluorophores in close proximity to TiO2 also showed a 40% increase in photostability due to a reduction of the excited-state lifetime.

  4. Phase object imaging inside the airy disc

    NASA Astrophysics Data System (ADS)

    Tychinsky, Vladimir P.

    1991-03-01

    The possibility of phase objects superresoluton imaging is theoretically justifieth The measurements with CPM " AIRYSCAN" showed the reality of O structures observations when the Airy disc di ameter i s 0 86 j. . m SUMMARY It has been known that the amount of information contained in the image of any object is mostly determined by the number of points measured i ndependentl y or by spati al resol uti on of the system. From the classic theory of the optical systems it follows that for noncoherent sources the -spatial resolution is limited by the aperture dd 6LX/N. A. ( Rayleigh criterion where X is wave length NA numerical aperture. ) The use of this criterion is equivalent tO the statement that any object inside the Airy disc of radius d that is the difraction image of a point is practical ly unresolved. However at the coherent illumination the intensity distribution in the image plane depends also upon the phase iq (r) of the wave scattered by the object and this is the basis of the Zernike method of phasecontrast microscopy differential interference contrast (DIC) and computer phase microscopy ( CPM ). In theoretical foundation of these methods there was no doubt in the correctness of Rayleigh criterion since the phase information is derived out of intensity distribution and as we know there were no experiments that disproved this

  5. Distributed Representation of Visual Objects by Single Neurons in the Human Brain

    PubMed Central

    Valdez, André B.; Papesh, Megan H.; Treiman, David M.; Smith, Kris A.; Goldinger, Stephen D.

    2015-01-01

    It remains unclear how single neurons in the human brain represent whole-object visual stimuli. While recordings in both human and nonhuman primates have shown distributed representations of objects (many neurons encoding multiple objects), recordings of single neurons in the human medial temporal lobe, taken as subjects' discriminated objects during multiple presentations, have shown gnostic representations (single neurons encoding one object). Because some studies suggest that repeated viewing may enhance neural selectivity for objects, we had human subjects discriminate objects in a single, more naturalistic viewing session. We found that, across 432 well isolated neurons recorded in the hippocampus and amygdala, the average fraction of objects encoded was 26%. We also found that more neurons encoded several objects versus only one object in the hippocampus (28 vs 18%, p < 0.001) and in the amygdala (30 vs 19%, p < 0.001). Thus, during realistic viewing experiences, typical neurons in the human medial temporal lobe code for a considerable range of objects, across multiple semantic categories. PMID:25834044

  6. Fuzzy object models for newborn brain MR image segmentation

    NASA Astrophysics Data System (ADS)

    Kobashi, Syoji; Udupa, Jayaram K.

    2013-03-01

    Newborn brain MR image segmentation is a challenging problem because of variety of size, shape and MR signal although it is the fundamental study for quantitative radiology in brain MR images. Because of the large difference between the adult brain and the newborn brain, it is difficult to directly apply the conventional methods for the newborn brain. Inspired by the original fuzzy object model introduced by Udupa et al. at SPIE Medical Imaging 2011, called fuzzy shape object model (FSOM) here, this paper introduces fuzzy intensity object model (FIOM), and proposes a new image segmentation method which combines the FSOM and FIOM into fuzzy connected (FC) image segmentation. The fuzzy object models are built from training datasets in which the cerebral parenchyma is delineated by experts. After registering FSOM with the evaluating image, the proposed method roughly recognizes the cerebral parenchyma region based on a prior knowledge of location, shape, and the MR signal given by the registered FSOM and FIOM. Then, FC image segmentation delineates the cerebral parenchyma using the fuzzy object models. The proposed method has been evaluated using 9 newborn brain MR images using the leave-one-out strategy. The revised age was between -1 and 2 months. Quantitative evaluation using false positive volume fraction (FPVF) and false negative volume fraction (FNVF) has been conducted. Using the evaluation data, a FPVF of 0.75% and FNVF of 3.75% were achieved. More data collection and testing are underway.

  7. Galaxies at z~7-8: z850-Dropouts in the Hubble Ultra Deep Field

    NASA Astrophysics Data System (ADS)

    Bouwens, R. J.; Thompson, R. I.; Illingworth, G. D.; Franx, M.; van Dokkum, P. G.; Fan, X.; Dickinson, M. E.; Eisenstein, D. J.; Rieke, M. J.

    2004-12-01

    We have detected likely z~7-8 galaxies in the 144''×144'' Near-Infrared Camera and Multi-Object Spectrometer (NICMOS) observations of the Hubble Ultra Deep Field. Objects are required to be >=3 σ detections in both NICMOS bands, J110 and H160. The selection criteria for this sample are (z850-J110)AB>0.8, (z850-J110)AB>0.66(J110-H160)AB+0.8, (J110-H160)AB<1.2 and no detection at less than 8500 Å. The five selected sources have total magnitudes H160,AB~27. Four of the five sources are quite blue compared to typical lower redshift dropout galaxies and are clustered within a 1 arcmin2 region. Because all five sources are near the limit of the NICMOS data, we have carefully evaluated their reality. Each of the candidates is visible in different splits of the data and a median stack. We analyzed several noise images and estimate the number of spurious sources to be 1+/-1. A search using an independent reduction of this same data set clearly revealed three of the five candidates and weakly detected a fourth candidate, suggesting that the contamination could be higher. For comparison with predictions from lower redshift samples, we take a conservative approach and adopt four z~7-8 galaxies as our sample. With the same detection criteria on simulated data sets, assuming no evolution from z~3.8, we predict 10 sources at z~7-8, or 14 if we use a more realistic (1+z)-1 size scaling. We estimate that the rest-frame continuum UV (~1800 Å) luminosity density at z~7.5 (integrated down to 0.3L*z=3) is just 0.20+0.12-0.08 times that found at z~3.8 (or 0.20+0.23-0.12 times this quantity including cosmic variance). Effectively this sets an upper limit on the luminosity density down to 0.3L*z=3 and is consistent with significant evolution at the bright end of the luminosity function from z~7.5 to 3.8. Even with the lower UV luminosity density at z~7.5, it appears that galaxies could still play an important role in reionization at these redshifts, although definitive measurements

  8. Toward Imaging of Small Objects with XUV Radiation

    NASA Astrophysics Data System (ADS)

    Sayrac, Muhammed; Kolomenski, Alexandre A.; Boran, Yakup; Schuessler, Hans

    The coherent diffraction imaging (CDI) technique has the potential to capture high resolution images of nano- or micron-sized structures when using XUV radiation obtained by high harmonic radiation (HHG) process. When a small object is exposed to XUV radiation, a diffraction pattern of the object is created. The advances in the coherent HHG enable obtaining photon flux sufficient for XUV imaging. The diffractive imaging technique from coherent table top XUV beams have made possible nanometer-scale resolution imaging by replacing the imaging optics with a computer reconstruction algorithm. In this study, we present our initial work on diffractive imaging using a tabletop XUV source. The initial investigation of imaging of a micron-sized mesh with an optimized HHG source is demonstrated. This work was supported in part by the Robert A. Welch Foundation Grant No. A1546 and the Qatar Foundation under the grant NPRP 8-735-1-154. M. Sayrac acknowledges support from the Ministry of National Education of the Republic of Turkey.

  9. Image Augmentation for Object Image Classification Based On Combination of Pre-Trained CNN and SVM

    NASA Astrophysics Data System (ADS)

    Shima, Yoshihiro

    2018-04-01

    Neural networks are a powerful means of classifying object images. The proposed image category classification method for object images combines convolutional neural networks (CNNs) and support vector machines (SVMs). A pre-trained CNN, called Alex-Net, is used as a pattern-feature extractor. Alex-Net is pre-trained for the large-scale object-image dataset ImageNet. Instead of training, Alex-Net, pre-trained for ImageNet is used. An SVM is used as trainable classifier. The feature vectors are passed to the SVM from Alex-Net. The STL-10 dataset are used as object images. The number of classes is ten. Training and test samples are clearly split. STL-10 object images are trained by the SVM with data augmentation. We use the pattern transformation method with the cosine function. We also apply some augmentation method such as rotation, skewing and elastic distortion. By using the cosine function, the original patterns were left-justified, right-justified, top-justified, or bottom-justified. Patterns were also center-justified and enlarged. Test error rate is decreased by 0.435 percentage points from 16.055% by augmentation with cosine transformation. Error rates are increased by other augmentation method such as rotation, skewing and elastic distortion, compared without augmentation. Number of augmented data is 30 times that of the original STL-10 5K training samples. Experimental test error rate for the test 8k STL-10 object images was 15.620%, which shows that image augmentation is effective for image category classification.

  10. Single molecule image formation, reconstruction and processing: introduction.

    PubMed

    Ashok, Amit; Piestun, Rafael; Stallinga, Sjoerd

    2016-07-01

    The ability to image at the single molecule scale has revolutionized research in molecular biology. This feature issue presents a collection of articles that provides new insights into the fundamental limits of single molecule imaging and reports novel techniques for image formation and analysis.

  11. A Ks-band-selected catalogue of objects in the ALHAMBRA survey

    NASA Astrophysics Data System (ADS)

    Nieves-Seoane, L.; Fernandez-Soto, A.; Arnalte-Mur, P.; Molino, A.; Stefanon, M.; Ferreras, I.; Ascaso, B.; Ballesteros, F. J.; Cristóbal-Hornillos, D.; López-Sanjuán, C.; Hurtado-Gil, Ll.; Márquez, I.; Masegosa, J.; Aguerri, J. A. L.; Alfaro, E.; Aparicio-Villegas, T.; Benítez, N.; Broadhurst, T.; Cabrera-Caño, J.; Castander, F. J.; Cepa, J.; Cerviño, M.; González Delgado, R. M.; Husillos, C.; Infante, L.; Martínez, V. J.; Moles, M.; Olmo, A. del; Perea, J.; Pović, M.; Prada, F.; Quintana, J. M.; Troncoso-Iribarren, P.; Viironen, K.

    2017-02-01

    The original ALHAMBRA catalogue contained over 400 000 galaxies selected using a synthetic F814W image, to the magnitude limit AB(F814W) ≈ 24.5. Given the photometric redshift depth of the ALHAMBRA multiband data (<z> = 0.86) and the approximately I-band selection, there is a noticeable bias against red objects at moderate redshift. We avoid this bias by creating a new catalogue selected in the Ks band. This newly obtained catalogue is certainly shallower in terms of apparent magnitude, but deeper in terms of redshift, with a significant population of red objects at z > 1. We select objects using the Ks band images, which reach an approximate AB magnitude limit Ks ≈ 22. We generate masks and derive completeness functions to characterize the sample. We have tested the quality of the photometry and photometric redshifts using both internal and external checks. Our final catalogue includes ≈95 000 sources down to Ks ≈ 22, with a significant tail towards high redshift. We have checked that there is a large sample of objects with spectral energy distributions that correspond to that of massive, passively evolving galaxies at z > 1, reaching as far as z ≈ 2.5. We have tested the possibility of combining our data with deep infrared observations at longer wavelengths, particularly Spitzer IRAC data.

  12. Single-pixel computational ghost imaging with helicity-dependent metasurface hologram.

    PubMed

    Liu, Hong-Chao; Yang, Biao; Guo, Qinghua; Shi, Jinhui; Guan, Chunying; Zheng, Guoxing; Mühlenbernd, Holger; Li, Guixin; Zentgraf, Thomas; Zhang, Shuang

    2017-09-01

    Different optical imaging techniques are based on different characteristics of light. By controlling the abrupt phase discontinuities with different polarized incident light, a metasurface can host a phase-only and helicity-dependent hologram. In contrast, ghost imaging (GI) is an indirect imaging modality to retrieve the object information from the correlation of the light intensity fluctuations. We report single-pixel computational GI with a high-efficiency reflective metasurface in both simulations and experiments. Playing a fascinating role in switching the GI target with different polarized light, the metasurface hologram generates helicity-dependent reconstructed ghost images and successfully introduces an additional security lock in a proposed optical encryption scheme based on the GI. The robustness of our encryption scheme is further verified with the vulnerability test. Building the first bridge between the metasurface hologram and the GI, our work paves the way to integrate their applications in the fields of optical communications, imaging technology, and security.

  13. Single-pixel computational ghost imaging with helicity-dependent metasurface hologram

    PubMed Central

    Liu, Hong-Chao; Yang, Biao; Guo, Qinghua; Shi, Jinhui; Guan, Chunying; Zheng, Guoxing; Mühlenbernd, Holger; Li, Guixin; Zentgraf, Thomas; Zhang, Shuang

    2017-01-01

    Different optical imaging techniques are based on different characteristics of light. By controlling the abrupt phase discontinuities with different polarized incident light, a metasurface can host a phase-only and helicity-dependent hologram. In contrast, ghost imaging (GI) is an indirect imaging modality to retrieve the object information from the correlation of the light intensity fluctuations. We report single-pixel computational GI with a high-efficiency reflective metasurface in both simulations and experiments. Playing a fascinating role in switching the GI target with different polarized light, the metasurface hologram generates helicity-dependent reconstructed ghost images and successfully introduces an additional security lock in a proposed optical encryption scheme based on the GI. The robustness of our encryption scheme is further verified with the vulnerability test. Building the first bridge between the metasurface hologram and the GI, our work paves the way to integrate their applications in the fields of optical communications, imaging technology, and security. PMID:28913433

  14. Distributed representation of visual objects by single neurons in the human brain.

    PubMed

    Valdez, André B; Papesh, Megan H; Treiman, David M; Smith, Kris A; Goldinger, Stephen D; Steinmetz, Peter N

    2015-04-01

    It remains unclear how single neurons in the human brain represent whole-object visual stimuli. While recordings in both human and nonhuman primates have shown distributed representations of objects (many neurons encoding multiple objects), recordings of single neurons in the human medial temporal lobe, taken as subjects' discriminated objects during multiple presentations, have shown gnostic representations (single neurons encoding one object). Because some studies suggest that repeated viewing may enhance neural selectivity for objects, we had human subjects discriminate objects in a single, more naturalistic viewing session. We found that, across 432 well isolated neurons recorded in the hippocampus and amygdala, the average fraction of objects encoded was 26%. We also found that more neurons encoded several objects versus only one object in the hippocampus (28 vs 18%, p < 0.001) and in the amygdala (30 vs 19%, p < 0.001). Thus, during realistic viewing experiences, typical neurons in the human medial temporal lobe code for a considerable range of objects, across multiple semantic categories. Copyright © 2015 the authors 0270-6474/15/355180-07$15.00/0.

  15. In vivo structure of the E. coli FtsZ-ring revealed by photoactivated localization microscopy (PALM).

    PubMed

    Fu, Guo; Huang, Tao; Buss, Jackson; Coltharp, Carla; Hensel, Zach; Xiao, Jie

    2010-09-13

    The FtsZ protein, a tubulin-like GTPase, plays a pivotal role in prokaryotic cell division. In vivo it localizes to the midcell and assembles into a ring-like structure-the Z-ring. The Z-ring serves as an essential scaffold to recruit all other division proteins and generates contractile force for cytokinesis, but its supramolecular structure remains unknown. Electron microscopy (EM) has been unsuccessful in detecting the Z-ring due to the dense cytoplasm of bacterial cells, and conventional fluorescence light microscopy (FLM) has only provided images with limited spatial resolution (200-300 nm) due to the diffraction of light. Hence, given the small sizes of bacteria cells, identifying the in vivo structure of the Z-ring presents a substantial challenge. Here, we used photoactivated localization microscopy (PALM), a single molecule-based super-resolution imaging technique, to characterize the in vivo structure of the Z-ring in E. coli. We achieved a spatial resolution of ∼35 nm and discovered that in addition to the expected ring-like conformation, the Z-ring of E. coli adopts a novel compressed helical conformation with variable helical length and pitch. We measured the thickness of the Z-ring to be ∼110 nm and the packing density of FtsZ molecules inside the Z-ring to be greater than what is expected for a single-layered flat ribbon configuration. Our results strongly suggest that the Z-ring is composed of a loose bundle of FtsZ protofilaments that randomly overlap with each other in both longitudinal and radial directions of the cell. Our results provide significant insight into the spatial organization of the Z-ring and open the door for further investigations of structure-function relationships and cell cycle-dependent regulation of the Z-ring.

  16. HIGHEST REDSHIFT IMAGE OF NEUTRAL HYDROGEN IN EMISSION: A CHILES DETECTION OF A STARBURSTING GALAXY AT z = 0.376

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fernández, Ximena; Gim, Hansung B.; Yun, Min S.

    2016-06-10

    Our current understanding of galaxy evolution still has many uncertainties associated with the details of the accretion, processing, and removal of gas across cosmic time. The next generation of radio telescopes will image the neutral hydrogen (H i) in galaxies over large volumes at high redshifts, which will provide key insights into these processes. We are conducting the COSMOS H i Large Extragalactic Survey (CHILES) with the Karl G. Jansky Very Large Array, which is the first survey to simultaneously observe H i from z = 0 to z ∼ 0.5. Here, we report the highest redshift H i 21more » cm detection in emission to date of the luminous infrared galaxy COSMOS J100054.83+023126.2 at z = 0.376 with the first 178 hr of CHILES data. The total H i mass is (2.9 ± 1.0) × 10{sup 10} M {sub ⊙} and the spatial distribution is asymmetric and extends beyond the galaxy. While optically the galaxy looks undisturbed, the H i distribution suggests an interaction with a candidate companion. In addition, we present follow-up Large Millimeter Telescope CO observations that show it is rich in molecular hydrogen, with a range of possible masses of (1.8–9.9) × 10{sup 10} M {sub ⊙}. This is the first study of the H i and CO in emission for a single galaxy beyond z ∼ 0.2.« less

  17. Objective evaluation of reconstruction methods for quantitative SPECT imaging in the absence of ground truth.

    PubMed

    Jha, Abhinav K; Song, Na; Caffo, Brian; Frey, Eric C

    2015-04-13

    Quantitative single-photon emission computed tomography (SPECT) imaging is emerging as an important tool in clinical studies and biomedical research. There is thus a need for optimization and evaluation of systems and algorithms that are being developed for quantitative SPECT imaging. An appropriate objective method to evaluate these systems is by comparing their performance in the end task that is required in quantitative SPECT imaging, such as estimating the mean activity concentration in a volume of interest (VOI) in a patient image. This objective evaluation can be performed if the true value of the estimated parameter is known, i.e. we have a gold standard. However, very rarely is this gold standard known in human studies. Thus, no-gold-standard techniques to optimize and evaluate systems and algorithms in the absence of gold standard are required. In this work, we developed a no-gold-standard technique to objectively evaluate reconstruction methods used in quantitative SPECT when the parameter to be estimated is the mean activity concentration in a VOI. We studied the performance of the technique with realistic simulated image data generated from an object database consisting of five phantom anatomies with all possible combinations of five sets of organ uptakes, where each anatomy consisted of eight different organ VOIs. Results indicate that the method provided accurate ranking of the reconstruction methods. We also demonstrated the application of consistency checks to test the no-gold-standard output.

  18. 3-dimensional imaging at nanometer resolutions

    DOEpatents

    Werner, James H.; Goodwin, Peter M.; Shreve, Andrew P.

    2010-03-09

    An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.

  19. Single and multiple object tracking using log-euclidean Riemannian subspace and block-division appearance model.

    PubMed

    Hu, Weiming; Li, Xi; Luo, Wenhan; Zhang, Xiaoqin; Maybank, Stephen; Zhang, Zhongfei

    2012-12-01

    Object appearance modeling is crucial for tracking objects, especially in videos captured by nonstationary cameras and for reasoning about occlusions between multiple moving objects. Based on the log-euclidean Riemannian metric on symmetric positive definite matrices, we propose an incremental log-euclidean Riemannian subspace learning algorithm in which covariance matrices of image features are mapped into a vector space with the log-euclidean Riemannian metric. Based on the subspace learning algorithm, we develop a log-euclidean block-division appearance model which captures both the global and local spatial layout information about object appearances. Single object tracking and multi-object tracking with occlusion reasoning are then achieved by particle filtering-based Bayesian state inference. During tracking, incremental updating of the log-euclidean block-division appearance model captures changes in object appearance. For multi-object tracking, the appearance models of the objects can be updated even in the presence of occlusions. Experimental results demonstrate that the proposed tracking algorithm obtains more accurate results than six state-of-the-art tracking algorithms.

  20. A curve fitting method for extrinsic camera calibration from a single image of a cylindrical object

    NASA Astrophysics Data System (ADS)

    Winkler, A. W.; Zagar, B. G.

    2013-08-01

    An important step in the process of optical steel coil quality assurance is to measure the proportions of width and radius of steel coils as well as the relative position and orientation of the camera. This work attempts to estimate these extrinsic parameters from single images by using the cylindrical coil itself as the calibration target. Therefore, an adaptive least-squares algorithm is applied to fit parametrized curves to the detected true coil outline in the acquisition. The employed model allows for strictly separating the intrinsic and the extrinsic parameters. Thus, the intrinsic camera parameters can be calibrated beforehand using available calibration software. Furthermore, a way to segment the true coil outline in the acquired images is motivated. The proposed optimization method yields highly accurate results and can be generalized even to measure other solids which cannot be characterized by the identification of simple geometric primitives.

  1. Single molecule localization imaging of exosomes using blinking silicon quantum dots

    NASA Astrophysics Data System (ADS)

    Zong, Shenfei; Zong, Junzhu; Chen, Chen; Jiang, Xiaoyue; Zhang, Yizhi; Wang, Zhuyuan; Cui, Yiping

    2018-02-01

    Discovering new fluorophores, which are suitable for single molecule localization microscopy (SMLM) is important for promoting the applications of SMLM in biological or material sciences. Here, we found that silicon quantum dots (Si QDs) possess a fluorescence blinking behavior, making them an excellent candidate for SMLM. The Si QDs are fabricated using a facile microwave-assisted method. Blinking of Si QDs is confirmed by single particle fluorescence measurement and the spatial resolution achieved is about 30 nm. To explore the potential application of Si QDs as the nanoprobes for SMLM imaging, cell derived exosomes are chosen as the object owing to their small size (50-100 nm in diameter). Since CD63 is commonly presented on the membrane of exosomes, CD63 aptamers are attached to the surface of Si QDs to form nanoprobes which can specifically recognize exosomes. SMLM imaging shows that Si QDs based nanoprobes can indeed realize super resolved optical imaging of exosomes. More importantly, blinking of Si QDs is observed in water or PBS buffer with no need for special imaging buffers. Besides, considering that silicon is highly biocompatible, Si QDs should have minimal cytotoxicity. These features make Si QDs quite suitable for SMLM applications especially for live cell imaging.

  2. Time-of-flight camera via a single-pixel correlation image sensor

    NASA Astrophysics Data System (ADS)

    Mao, Tianyi; Chen, Qian; He, Weiji; Dai, Huidong; Ye, Ling; Gu, Guohua

    2018-04-01

    A time-of-flight imager based on single-pixel correlation image sensors is proposed for noise-free depth map acquisition in presence of ambient light. Digital micro-mirror device and time-modulated IR-laser provide spatial and temporal illumination on the unknown object. Compressed sensing and ‘four bucket principle’ method are combined to reconstruct the depth map from a sequence of measurements at a low sampling rate. Second-order correlation transform is also introduced to reduce the noise from the detector itself and direct ambient light. Computer simulations are presented to validate the computational models and improvement of reconstructions.

  3. Neural Imaging Using Single-Photon Avalanche Diodes

    PubMed Central

    Karami, Mohammad Azim; Ansarian, Misagh

    2017-01-01

    Introduction: This paper analyses the ability of single-photon avalanche diodes (SPADs) for neural imaging. The current trend in the production of SPADs moves toward the minimum dark count rate (DCR) and maximum photon detection probability (PDP). Moreover, the jitter response which is the main measurement characteristic for the timing uncertainty is progressing. Methods: The neural imaging process using SPADs can be performed by means of florescence lifetime imaging (FLIM), time correlated single-photon counting (TCSPC), positron emission tomography (PET), and single-photon emission computed tomography (SPECT). Results: This trend will result in more precise neural imaging cameras. While achieving low DCR SPADs is difficult in deep submicron technologies because of using higher doping profiles, higher PDPs are reported in green and blue part of light. Furthermore, the number of pixels integrated in the same chip is increasing with the technology progress which can result in the higher resolution of imaging. Conclusion: This study proposes implemented SPADs in Deep-submicron technologies to be used in neural imaging cameras, due to the small size pixels and higher timing accuracies. PMID:28446946

  4. Object-Part Attention Model for Fine-Grained Image Classification

    NASA Astrophysics Data System (ADS)

    Peng, Yuxin; He, Xiangteng; Zhao, Junjie

    2018-03-01

    Fine-grained image classification is to recognize hundreds of subcategories belonging to the same basic-level category, such as 200 subcategories belonging to the bird, which is highly challenging due to large variance in the same subcategory and small variance among different subcategories. Existing methods generally first locate the objects or parts and then discriminate which subcategory the image belongs to. However, they mainly have two limitations: (1) Relying on object or part annotations which are heavily labor consuming. (2) Ignoring the spatial relationships between the object and its parts as well as among these parts, both of which are significantly helpful for finding discriminative parts. Therefore, this paper proposes the object-part attention model (OPAM) for weakly supervised fine-grained image classification, and the main novelties are: (1) Object-part attention model integrates two level attentions: object-level attention localizes objects of images, and part-level attention selects discriminative parts of object. Both are jointly employed to learn multi-view and multi-scale features to enhance their mutual promotions. (2) Object-part spatial constraint model combines two spatial constraints: object spatial constraint ensures selected parts highly representative, and part spatial constraint eliminates redundancy and enhances discrimination of selected parts. Both are jointly employed to exploit the subtle and local differences for distinguishing the subcategories. Importantly, neither object nor part annotations are used in our proposed approach, which avoids the heavy labor consumption of labeling. Comparing with more than 10 state-of-the-art methods on 4 widely-used datasets, our OPAM approach achieves the best performance.

  5. An object-based storage model for distributed remote sensing images

    NASA Astrophysics Data System (ADS)

    Yu, Zhanwu; Li, Zhongmin; Zheng, Sheng

    2006-10-01

    It is very difficult to design an integrated storage solution for distributed remote sensing images to offer high performance network storage services and secure data sharing across platforms using current network storage models such as direct attached storage, network attached storage and storage area network. Object-based storage, as new generation network storage technology emerged recently, separates the data path, the control path and the management path, which solves the bottleneck problem of metadata existed in traditional storage models, and has the characteristics of parallel data access, data sharing across platforms, intelligence of storage devices and security of data access. We use the object-based storage in the storage management of remote sensing images to construct an object-based storage model for distributed remote sensing images. In the storage model, remote sensing images are organized as remote sensing objects stored in the object-based storage devices. According to the storage model, we present the architecture of a distributed remote sensing images application system based on object-based storage, and give some test results about the write performance comparison of traditional network storage model and object-based storage model.

  6. Impulse radar imaging system for concealed object detection

    NASA Astrophysics Data System (ADS)

    Podd, F. J. W.; David, M.; Iqbal, G.; Hussain, F.; Morris, D.; Osakue, E.; Yeow, Y.; Zahir, S.; Armitage, D. W.; Peyton, A. J.

    2013-10-01

    Electromagnetic systems for imaging concealed objects at checkpoints typically employ radiation at millimetre and terahertz frequencies. These systems have been shown to be effective and provide a sufficiently high resolution image. However there are difficulties and current electromagnetic systems have limitations particularly in accurately differentiating between threat and innocuous objects based on shape, surface emissivity or reflectivity, which are indicative parameters. In addition, water has a high absorption coefficient at millimetre wavelength and terahertz frequencies, which makes it more difficult for these frequencies to image through thick damp clothing. This paper considers the potential of using ultra wideband (UWB) in the low gigahertz range. The application of this frequency band to security screening appears to be a relatively new field. The business case for implementing the UWB system has been made financially viable by the recent availability of low-cost integrated circuits operating at these frequencies. Although designed for the communication sector, these devices can perform the required UWB radar measurements as well. This paper reports the implementation of a 2 to 5 GHz bandwidth linear array scanner. The paper describes the design and fabrication of transmitter and receiver antenna arrays whose individual elements are a type of antipodal Vivaldi antenna. The antenna's frequency and angular response were simulated in CST Microwave Studio and compared with laboratory measurements. The data pre-processing methods of background subtraction and deconvolution are implemented to improve the image quality. The background subtraction method uses a reference dataset to remove antenna crosstalk and room reflections from the dataset. The deconvolution method uses a Wiener filter to "sharpen" the returned echoes which improves the resolution of the reconstructed image. The filter uses an impulse response reference dataset and a signal

  7. Imaging, object detection, and change detection with a polarized multistatic GPR array

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Beer, N. Reginald; Paglieroni, David W.

    A polarized detection system performs imaging, object detection, and change detection factoring in the orientation of an object relative to the orientation of transceivers. The polarized detection system may operate on one of several modes of operation based on whether the imaging, object detection, or change detection is performed separately for each transceiver orientation. In combined change mode, the polarized detection system performs imaging, object detection, and change detection separately for each transceiver orientation, and then combines changes across polarizations. In combined object mode, the polarized detection system performs imaging and object detection separately for each transceiver orientation, and thenmore » combines objects across polarizations and performs change detection on the result. In combined image mode, the polarized detection system performs imaging separately for each transceiver orientation, and then combines images across polarizations and performs object detection followed by change detection on the result.« less

  8. Imaging objects behind small obstacles using axicon lens

    NASA Astrophysics Data System (ADS)

    Perinchery, Sandeep M.; Shinde, Anant; Murukeshan, V. M.

    2017-06-01

    Axicon lenses are conical prisms, which are known to focus a light source to a line comprising of multiple points along the optical axis. In this study, we analyze the potential of axicon lenses to view, image and record the object behind opaque obstacles in free space. The advantage of an axicon lens over a regular lens is demonstrated experimentally. Parameters such as obstacle size, object and the obstacle position in the context of imaging behind obstacles are tested using Zemax optical simulation. This proposed concept can be easily adapted to most of the optical imaging methods and microscopy modalities.

  9. Passive Infrared Thermographic Imaging for Mobile Robot Object Identification

    NASA Astrophysics Data System (ADS)

    Hinders, M. K.; Fehlman, W. L.

    2010-02-01

    The usefulness of thermal infrared imaging as a mobile robot sensing modality is explored, and a set of thermal-physical features used to characterize passive thermal objects in outdoor environments is described. Objects that extend laterally beyond the thermal camera's field of view, such as brick walls, hedges, picket fences, and wood walls as well as compact objects that are laterally within the thermal camera's field of view, such as metal poles and tree trunks, are considered. Classification of passive thermal objects is a subtle process since they are not a source for their own emission of thermal energy. A detailed analysis is included of the acquisition and preprocessing of thermal images, as well as the generation and selection of thermal-physical features from these objects within thermal images. Classification performance using these features is discussed, as a precursor to the design of a physics-based model to automatically classify these objects.

  10. Wavelet Fusion for Concealed Object Detection Using Passive Millimeter Wave Sequence Images

    NASA Astrophysics Data System (ADS)

    Chen, Y.; Pang, L.; Liu, H.; Xu, X.

    2018-04-01

    PMMW imaging system can create interpretable imagery on the objects concealed under clothing, which gives the great advantage to the security check system. Paper addresses wavelet fusion to detect concealed objects using passive millimeter wave (PMMW) sequence images. According to PMMW real-time imager acquired image characteristics and storage methods firstly, using the sum of squared difference (SSD) as the image-related parameters to screen the sequence images. Secondly, the selected images are optimized using wavelet fusion algorithm. Finally, the concealed objects are detected by mean filter, threshold segmentation and edge detection. The experimental results show that this method improves the detection effect of concealed objects by selecting the most relevant images from PMMW sequence images and using wavelet fusion to enhance the information of the concealed objects. The method can be effectively applied to human body concealed object detection in millimeter wave video.

  11. Quantitative analysis of single-molecule superresolution images

    PubMed Central

    Coltharp, Carla; Yang, Xinxing; Xiao, Jie

    2014-01-01

    This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10 – 50 nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images. PMID:25179006

  12. Microwave imaging of spinning object using orbital angular momentum

    NASA Astrophysics Data System (ADS)

    Liu, Kang; Li, Xiang; Gao, Yue; Wang, Hongqiang; Cheng, Yongqiang

    2017-09-01

    The linear Doppler shift used for the detection of a spinning object becomes significantly weakened when the line of sight (LOS) is perpendicular to the object, which will result in the failure of detection. In this paper, a new detection and imaging technique for spinning objects is developed. The rotational Doppler phenomenon is observed by using the microwave carrying orbital angular momentum (OAM). To converge the radiation energy on the area where objects might exist, the generation method of OAM beams is proposed based on the frequency diversity principle, and the imaging model is derived accordingly. The detection method of the rotational Doppler shift and the imaging approach of the azimuthal profiles are proposed, which are verified by proof-of-concept experiments. Simulation and experimental results demonstrate that OAM beams can still be used to obtain the azimuthal profiles of spinning objects even when the LOS is perpendicular to the object. This work remedies the insufficiency in existing microwave sensing technology and offers a new solution to the object identification problem.

  13. Crystal and electronic structures, luminescence properties of Eu 2+-doped Si 6-zAl zO zN 8-z and M ySi 6-zAl z-yO z+yN 8-z-y ( M=2Li, Mg, Ca, Sr, Ba)

    NASA Astrophysics Data System (ADS)

    Li, Y. Q.; Hirosaki, N.; Xie, R. J.; Takeda, T.; Mitomo, M.

    2008-12-01

    The crystal structure, electronic structure, and photoluminescence properties of Eu xSi 6-zAl z-xO z+xN 8-z-x ( x=0-0.1, 0< z<1) and Eu xM ySi 6-zAl z-x-yO z+x+yN 8-z-x-y ( M=2Li, Mg, Ca, Sr, Ba) have been studied. Single-phase Eu xSi 6-zAl z-xO z+xN 8-z-x can be obtained in very narrow ranges of x⩽0.06 ( z=0.15) and z<0.5 ( x=0.3), indicating that limited Eu 2+ ions can be incorporated into nitrogen-rich Si 6-zAl zO zN 8-z. The Eu 2+ ion is found to occupy the 2 b site in a hexagonal unit cell ( P6 3/ m) and directly connected by six adjacent nitrogen/oxygen atoms ranging 2.4850-2.5089 Å. The calculated host band gaps by the relativistic DV-X α method are about 5.55 and 5.45 eV (without Eu 2+ 4 f5 d levels) for x=0 and 0.013 in Eu xSi 6-zAl z-xO z+xN 8-z-x ( z=0.15), in which the top of the 5 d orbitals overlap with the Si-3 s3 p and N-2 p orbitals within the bottom of the conduction band of the host. Eu xSi 6-zAl z-xO z+xN 8-z-x shows a strong green emission with a broad Eu 2+ band centered at about 530 nm under UV to near-UV excitation range. The excitation and emission spectra are hardly modified by Eu concentration and dual-doping ions of Li and other alkaline-earth ions with Eu. Higher Eu concentrations can significantly quench the luminescence of Eu 2+ and decrease the thermal quenching temperature. In addition, the emission spectrum can only be slightly tuned to the longer wavelengths (˜529-545 nm) by increasing z within the solid solution range of z<0.5. Furthermore, the luminescence intensity of Eu xSi 6-zAl z-xO z+xN 8-z-x can be improved by increasing z and the dual-doping of Li and Ba.

  14. Adaptive Optics For Imaging Bright Objects Next To Dim Ones

    NASA Technical Reports Server (NTRS)

    Shao, Michael; Yu, Jeffrey W.; Malbet, Fabien

    1996-01-01

    Adaptive optics used in imaging optical systems, according to proposal, to enhance high-dynamic-range images (images of bright objects next to dim objects). Designed to alter wavefronts to correct for effects of scattering of light from small bumps on imaging optics. Original intended application of concept in advanced camera installed on Hubble Space Telescope for imaging of such phenomena as large planets near stars other than Sun. Also applicable to other high-quality telescopes and cameras.

  15. High dynamic range imaging by pupil single-mode filtering and remapping

    NASA Astrophysics Data System (ADS)

    Perrin, G.; Lacour, S.; Woillez, J.; Thiébaut, É.

    2006-12-01

    Because of atmospheric turbulence, obtaining high angular resolution images with a high dynamic range is difficult even in the near-infrared domain of wavelengths. We propose a novel technique to overcome this issue. The fundamental idea is to apply techniques developed for long baseline interferometry to the case of a single-aperture telescope. The pupil of the telescope is broken down into coherent subapertures each feeding a single-mode fibre. A remapping of the exit pupil allows interfering all subapertures non-redundantly. A diffraction-limited image with very high dynamic range is reconstructed from the fringe pattern analysis with aperture synthesis techniques, free of speckle noise. The performances of the technique are demonstrated with simulations in the visible range with an 8-m telescope. Raw dynamic ranges of 1:106 can be obtained in only a few tens of seconds of integration time for bright objects.

  16. Recognizing 3-D Objects Using 2-D Images

    DTIC Science & Technology

    1993-05-01

    also depends on models that contain significant numbers of viewpoint-invariant features, such as parallelograms. Biederman [9] built on Lowe’s work to...objects. Biederman suggests that we recognize images of objects by dividing the image into a few parts, called geons. Each geon is described by the...are also described with a few view-invariant features. Together, these provide a set of features 1.3. STRATEG;IES FOR INDEXING 25 which Biederman

  17. Current Status of Single Particle Imaging with X-ray Lasers

    DOE PAGES

    Sun, Zhibin; Fan, Jiadong; Li, Haoyuan; ...

    2018-01-22

    The advent of ultrafast X-ray free-electron lasers (XFELs) opens the tantalizing possibility of the atomic-resolution imaging of reproducible objects such as viruses, nanoparticles, single molecules, clusters, and perhaps biological cells, achieving a resolution for single particle imaging better than a few tens of nanometers. Improving upon this is a significant challenge which has been the focus of a global single particle imaging (SPI) initiative launched in December 2014 at the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, USA. A roadmap was outlined, and significant multi-disciplinary effort has since been devoted to work on the technical challenges of SPImore » such as radiation damage, beam characterization, beamline instrumentation and optics, sample preparation and delivery and algorithm development at multiple institutions involved in the SPI initiative. Currently, the SPI initiative has achieved 3D imaging of rice dwarf virus (RDV) and coliphage PR772 viruses at ~10 nm resolution by using soft X-ray FEL pulses at the Atomic Molecular and Optical (AMO) instrument of LCLS. Meanwhile, diffraction patterns with signal above noise up to the corner of the detector with a resolution of ~6 Ångström (Å) were also recorded with hard X-rays at the Coherent X-ray Imaging (CXI) instrument, also at LCLS. Achieving atomic resolution is truly a grand challenge and there is still a long way to go in light of recent developments in electron microscopy. However, the potential for studying dynamics at physiological conditions and capturing ultrafast biological, chemical and physical processes represents a tremendous potential application, attracting continued interest in pursuing further method development. In this paper, we give a brief introduction of SPI developments and look ahead to further method development.« less

  18. Current Status of Single Particle Imaging with X-ray Lasers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Sun, Zhibin; Fan, Jiadong; Li, Haoyuan

    The advent of ultrafast X-ray free-electron lasers (XFELs) opens the tantalizing possibility of the atomic-resolution imaging of reproducible objects such as viruses, nanoparticles, single molecules, clusters, and perhaps biological cells, achieving a resolution for single particle imaging better than a few tens of nanometers. Improving upon this is a significant challenge which has been the focus of a global single particle imaging (SPI) initiative launched in December 2014 at the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, USA. A roadmap was outlined, and significant multi-disciplinary effort has since been devoted to work on the technical challenges of SPImore » such as radiation damage, beam characterization, beamline instrumentation and optics, sample preparation and delivery and algorithm development at multiple institutions involved in the SPI initiative. Currently, the SPI initiative has achieved 3D imaging of rice dwarf virus (RDV) and coliphage PR772 viruses at ~10 nm resolution by using soft X-ray FEL pulses at the Atomic Molecular and Optical (AMO) instrument of LCLS. Meanwhile, diffraction patterns with signal above noise up to the corner of the detector with a resolution of ~6 Ångström (Å) were also recorded with hard X-rays at the Coherent X-ray Imaging (CXI) instrument, also at LCLS. Achieving atomic resolution is truly a grand challenge and there is still a long way to go in light of recent developments in electron microscopy. However, the potential for studying dynamics at physiological conditions and capturing ultrafast biological, chemical and physical processes represents a tremendous potential application, attracting continued interest in pursuing further method development. In this paper, we give a brief introduction of SPI developments and look ahead to further method development.« less

  19. A single-image method for x-ray refractive index CT.

    PubMed

    Mittone, A; Gasilov, S; Brun, E; Bravin, A; Coan, P

    2015-05-07

    X-ray refraction-based computer tomography imaging is a well-established method for nondestructive investigations of various objects. In order to perform the 3D reconstruction of the index of refraction, two or more raw computed tomography phase-contrast images are usually acquired and combined to retrieve the refraction map (i.e. differential phase) signal within the sample. We suggest an approximate method to extract the refraction signal, which uses a single raw phase-contrast image. This method, here applied to analyzer-based phase-contrast imaging, is employed to retrieve the index of refraction map of a biological sample. The achieved accuracy in distinguishing the different tissues is comparable with the non-approximated approach. The suggested procedure can be used for precise refraction computer tomography with the advantage of a reduction of at least a factor of two of both the acquisition time and the dose delivered to the sample with respect to any of the other algorithms in the literature.

  20. Superresolution Imaging using Single-Molecule Localization

    PubMed Central

    Patterson, George; Davidson, Michael; Manley, Suliana; Lippincott-Schwartz, Jennifer

    2013-01-01

    Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (∼10–20-nm resolution), allowing biological processes to be described at the molecular scale. Here, we discuss a form of superresolution microscopy based on the controlled activation and sampling of sparse subsets of photoconvertible fluorescent molecules. In this single-molecule based imaging approach, a wide variety of probes have proved valuable, ranging from genetically encodable photoactivatable fluorescent proteins to photoswitchable cyanine dyes. These have been used in diverse applications of superresolution imaging: from three-dimensional, multicolor molecule localization to tracking of nanometric structures and molecules in living cells. Single-molecule-based superresolution imaging thus offers exciting possibilities for obtaining molecular-scale information on biological events occurring at variable timescales. PMID:20055680

  1. Analysis of objects in binary images. M.S. Thesis - Old Dominion Univ.

    NASA Technical Reports Server (NTRS)

    Leonard, Desiree M.

    1991-01-01

    Digital image processing techniques are typically used to produce improved digital images through the application of successive enhancement techniques to a given image or to generate quantitative data about the objects within that image. In support of and to assist researchers in a wide range of disciplines, e.g., interferometry, heavy rain effects on aerodynamics, and structure recognition research, it is often desirable to count objects in an image and compute their geometric properties. Therefore, an image analysis application package, focusing on a subset of image analysis techniques used for object recognition in binary images, was developed. This report describes the techniques and algorithms utilized in three main phases of the application and are categorized as: image segmentation, object recognition, and quantitative analysis. Appendices provide supplemental formulas for the algorithms employed as well as examples and results from the various image segmentation techniques and the object recognition algorithm implemented.

  2. Atomic scale structure and chemistry of interfaces by Z-contrast imaging and electron energy loss spectroscopy in the stem

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McGibbon, M.M.; Browning, N.D.; Chisholm, M.F.

    The macroscopic properties of many materials are controlled by the structure and chemistry at grain boundaries. A basic understanding of the structure-property relationship requires a technique which probes both composition and chemical bonding on an atomic scale. High-resolution Z-contrast imaging in the scanning transmission electron microscope (STEM) forms an incoherent image in which changes in atomic structure and composition across an interface can be interpreted directly without the need for preconceived atomic structure models. Since the Z-contrast image is formed by electrons scattered through high angles, parallel detection electron energy loss spectroscopy (PEELS) can be used simultaneously to provide complementarymore » chemical information on an atomic scale. The fine structure in the PEEL spectra can be used to investigate the local electronic structure and the nature of the bonding across the interface. In this paper we use the complimentary techniques of high resolution Z-contrast imaging and PEELS to investigate the atomic structure and chemistry of a 25{degree} symmetric tilt boundary in a bicrystal of the electroceramic SrTiO{sub 3}.« less

  3. Single-pixel imaging by Hadamard transform and its application for hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Mizutani, Yasuhiro; Shibuya, Kyuki; Taguchi, Hiroki; Iwata, Tetsuo; Takaya, Yasuhiro; Yasui, Takeshi

    2016-10-01

    In this paper, we report on comparisons of single-pixel imagings using Hadamard Transform (HT) and the ghost imaging (GI) in the view point of the visibility under weak light conditions. For comparing the two methods, we have discussed about qualities of images based on experimental results and numerical analysis. To detect images by the TH method, we have illuminated the Hadamard-pattern mask and calculated by orthogonal transform. On the other hand, the GH method can detect images by illuminating random patterns and a correlation measurement. For comparing two methods under weak light intensity, we have controlled illuminated intensities of a DMD projector about 0.1 in signal-to-noise ratio. Though a process speed of the HT image was faster then an image via the GI, the GI method has an advantage of detection under weak light condition. An essential difference between the HT and the GI method is discussed about reconstruction process. Finally, we also show a typical application of the single-pixel imaging such as hyperspectral images by using dual-optical frequency combs. An optical setup consists of two fiber lasers, spatial light modulated for generating patten illumination, and a single pixel detector. We are successful to detect hyperspectrul images in a range from 1545 to 1555 nm at 0.01nm resolution.

  4. Direct Three-Dimensional Myocardial Strain Tensor Quantification and Tracking using zHARP★

    PubMed Central

    Abd-Elmoniem, Khaled Z.; Stuber, Matthias; Prince, Jerry L.

    2008-01-01

    Images of myocardial strain can be used to diagnose heart disease, plan and monitor treatment, and to learn about cardiac structure and function. Three-dimensional (3-D) strain is typically quantified using many magnetic resonance (MR) images obtained in two or three orthogonal planes. Problems with this approach include long scan times, image misregistration, and through-plane motion. This article presents a novel method for calculating cardiac 3-D strain using a stack of two or more images acquired in only one orientation. The zHARP pulse sequence encodes in-plane motion using MR tagging and out-of-plane motion using phase encoding, and has been previously shown to be capable of computing 3D displacement within a single image plane. Here, data from two adjacent image planes are combined to yield a 3-D strain tensor at each pixel; stacks of zHARP images can be used to derive stacked arrays of 3D strain tensors without imaging multiple orientations and without numerical interpolation. The performance and accuracy of the method is demonstrated in-vitro on a phantom and in-vivo in four healthy adult human subjects. PMID:18511332

  5. Z-Index Parameterization for Volumetric CT Image Reconstruction via 3-D Dictionary Learning.

    PubMed

    Bai, Ti; Yan, Hao; Jia, Xun; Jiang, Steve; Wang, Ge; Mou, Xuanqin

    2017-12-01

    Despite the rapid developments of X-ray cone-beam CT (CBCT), image noise still remains a major issue for the low dose CBCT. To suppress the noise effectively while retain the structures well for low dose CBCT image, in this paper, a sparse constraint based on the 3-D dictionary is incorporated into a regularized iterative reconstruction framework, defining the 3-D dictionary learning (3-DDL) method. In addition, by analyzing the sparsity level curve associated with different regularization parameters, a new adaptive parameter selection strategy is proposed to facilitate our 3-DDL method. To justify the proposed method, we first analyze the distributions of the representation coefficients associated with the 3-D dictionary and the conventional 2-D dictionary to compare their efficiencies in representing volumetric images. Then, multiple real data experiments are conducted for performance validation. Based on these results, we found: 1) the 3-D dictionary-based sparse coefficients have three orders narrower Laplacian distribution compared with the 2-D dictionary, suggesting the higher representation efficiencies of the 3-D dictionary; 2) the sparsity level curve demonstrates a clear Z-shape, and hence referred to as Z-curve, in this paper; 3) the parameter associated with the maximum curvature point of the Z-curve suggests a nice parameter choice, which could be adaptively located with the proposed Z-index parameterization (ZIP) method; 4) the proposed 3-DDL algorithm equipped with the ZIP method could deliver reconstructions with the lowest root mean squared errors and the highest structural similarity index compared with the competing methods; 5) similar noise performance as the regular dose FDK reconstruction regarding the standard deviation metric could be achieved with the proposed method using (1/2)/(1/4)/(1/8) dose level projections. The contrast-noise ratio is improved by ~2.5/3.5 times with respect to two different cases under the (1/8) dose level compared

  6. Evolution in the Continuum Morphological Properties of Ly alpha-Emitting Galaxies from Z=3.1 to Z=2.1

    NASA Technical Reports Server (NTRS)

    Bond, Nicholas A.; Gawiser, Eric; Guaita, Lucia; Padilla, Nelson; Gronwall, Chile Caryl; Ciardullo, Robin; Lai, Kamson

    2011-01-01

    We present a rest-frame ultraviolet morphological analysis of 108 z = 2.1 Lyman Alpha Emitters (LAEs) in the Extended Chandra Deep Field South (ECDF-S) and compare it to a similar sample of 171 LAEs at z = 3.1 . Using Hubble Space Telescope (HST) images taken as part of the Galaxy Evolution From Morphology and SEDs survey, Great Observatories Origins Deep Survey, and Hubble Ultradeep Field surveys, we measure the size and photometric component distributions, where photo- metric components are defined as distinct clumps of UV-continuum emission. At both redshifts, the majority of LAEs have observed half-light radii < 2 kpc, but the median half-light radius rises from 0.97 kpc at z = 3.1 to 1.41 kpc at z = 2.1. A similar evolution is seen in the sizes of individual rest-UV components, but there is no evidence for evolution in the number of mUlti-component systems. In the z = 2.1 LAE sample, we see clear correlations between the LAE size and other physical properties derived from its SED. LAEs are found to be larger for galaxies with larger stellar mass, larger star formation rate, and larger dust obscuration, but there is no evidence for a trend between equivalent width and half-light radius at either redshift. The presence of these correlations suggests that a wide range of objects are being selected by LAE surveys at that redshift, including a significant fraction of objects for which a massive and moderately extended population of old stars underlies the young starburst giving rise to the Lya emission.

  7. Sensing a buried resonant object by single-channel time reversal.

    PubMed

    Waters, Zachary J; Dzikowicz, Benjamin R; Holt, R Glynn; Roy, Ronald A

    2009-07-01

    Scaled laboratory experiments are conducted to assess the efficacy of iterative, single-channel time reversal for enhancement of monostatic returns from resonant spheres in the free field and buried in a sediment phantom. Experiments are performed in a water tank using a broad-band piston transducer operating between 0.4 and 1.5 MHz and calibrated using free surface reflections. Solid and hollow metallic spheres, 6.35 mm in diameter, are buried in a consolidation of 128-microm-mean- diameter spherical glass beads. The procedure consists of exciting the target object with a broadband pulse, sampling the return using a finite time window, reversing the signal in time, and using this reversed signal as the source waveform for the next interrogation. Results indicate that the spectrum of the returns rapidly converges to the dominant mode in the backscattering response of the target. Signal-to-noise enhancement of the target echo is demonstrated for a target at several burial depths. Images generated by scanning the transducer over the location of multiple buried targets demonstrate the ability of the technique to distinguish between targets of differing type and to yield an enhancement of different modes within the response of a single target as a function of transducer position and processing bandwidth.

  8. An efficient direct method for image registration of flat objects

    NASA Astrophysics Data System (ADS)

    Nikolaev, Dmitry; Tihonkih, Dmitrii; Makovetskii, Artyom; Voronin, Sergei

    2017-09-01

    Image alignment of rigid surfaces is a rapidly developing area of research and has many practical applications. Alignment methods can be roughly divided into two types: feature-based methods and direct methods. Known SURF and SIFT algorithms are examples of the feature-based methods. Direct methods refer to those that exploit the pixel intensities without resorting to image features and image-based deformations are general direct method to align images of deformable objects in 3D space. Nevertheless, it is not good for the registration of images of 3D rigid objects since the underlying structure cannot be directly evaluated. In the article, we propose a model that is suitable for image alignment of rigid flat objects under various illumination models. The brightness consistency assumptions used for reconstruction of optimal geometrical transformation. Computer simulation results are provided to illustrate the performance of the proposed algorithm for computing of an accordance between pixels of two images.

  9. Extended depth of field imaging for high speed object analysis

    NASA Technical Reports Server (NTRS)

    Frost, Keith (Inventor); Ortyn, William (Inventor); Basiji, David (Inventor); Bauer, Richard (Inventor); Liang, Luchuan (Inventor); Hall, Brian (Inventor); Perry, David (Inventor)

    2011-01-01

    A high speed, high-resolution flow imaging system is modified to achieve extended depth of field imaging. An optical distortion element is introduced into the flow imaging system. Light from an object, such as a cell, is distorted by the distortion element, such that a point spread function (PSF) of the imaging system is invariant across an extended depth of field. The distorted light is spectrally dispersed, and the dispersed light is used to simultaneously generate a plurality of images. The images are detected, and image processing is used to enhance the detected images by compensating for the distortion, to achieve extended depth of field images of the object. The post image processing preferably involves de-convolution, and requires knowledge of the PSF of the imaging system, as modified by the optical distortion element.

  10. SHERPA: an image segmentation and outline feature extraction tool for diatoms and other objects.

    PubMed

    Kloster, Michael; Kauer, Gerhard; Beszteri, Bánk

    2014-06-25

    Light microscopic analysis of diatom frustules is widely used both in basic and applied research, notably taxonomy, morphometrics, water quality monitoring and paleo-environmental studies. In these applications, usually large numbers of frustules need to be identified and/or measured. Although there is a need for automation in these applications, and image processing and analysis methods supporting these tasks have previously been developed, they did not become widespread in diatom analysis. While methodological reports for a wide variety of methods for image segmentation, diatom identification and feature extraction are available, no single implementation combining a subset of these into a readily applicable workflow accessible to diatomists exists. The newly developed tool SHERPA offers a versatile image processing workflow focused on the identification and measurement of object outlines, handling all steps from image segmentation over object identification to feature extraction, and providing interactive functions for reviewing and revising results. Special attention was given to ease of use, applicability to a broad range of data and problems, and supporting high throughput analyses with minimal manual intervention. Tested with several diatom datasets from different sources and of various compositions, SHERPA proved its ability to successfully analyze large amounts of diatom micrographs depicting a broad range of species. SHERPA is unique in combining the following features: application of multiple segmentation methods and selection of the one giving the best result for each individual object; identification of shapes of interest based on outline matching against a template library; quality scoring and ranking of resulting outlines supporting quick quality checking; extraction of a wide range of outline shape descriptors widely used in diatom studies and elsewhere; minimizing the need for, but enabling manual quality control and corrections. Although primarily

  11. Subaru High-z Exploration of Low-Luminosity Quasars (SHELLQs). II. Discovery of 32 quasars and luminous galaxies at 5.7 < z ≤ 6.8

    NASA Astrophysics Data System (ADS)

    Matsuoka, Yoshiki; Onoue, Masafusa; Kashikawa, Nobunari; Iwasawa, Kazushi; Strauss, Michael A.; Nagao, Tohru; Imanishi, Masatoshi; Lee, Chien-Hsiu; Akiyama, Masayuki; Asami, Naoko; Bosch, James; Foucaud, Sébastien; Furusawa, Hisanori; Goto, Tomotsugu; Gunn, James E.; Harikane, Yuichi; Ikeda, Hiroyuki; Izumi, Takuma; Kawaguchi, Toshihiro; Kikuta, Satoshi; Kohno, Kotaro; Komiyama, Yutaka; Lupton, Robert H.; Minezaki, Takeo; Miyazaki, Satoshi; Morokuma, Tomoki; Murayama, Hitoshi; Niida, Mana; Nishizawa, Atsushi J.; Oguri, Masamune; Ono, Yoshiaki; Ouchi, Masami; Price, Paul A.; Sameshima, Hiroaki; Schulze, Andreas; Shirakata, Hikari; Silverman, John D.; Sugiyama, Naoshi; Tait, Philip J.; Takada, Masahiro; Takata, Tadafumi; Tanaka, Masayuki; Tang, Ji-Jia; Toba, Yoshiki; Utsumi, Yousuke; Wang, Shiang-Yu

    2018-01-01

    We present spectroscopic identification of 32 new quasars and luminous galaxies discovered at 5.7 < z ≤ 6.8. This is the second in a series of papers presenting the results of the Subaru High-z Exploration of Low-Luminosity Quasars (SHELLQs) project, which exploits the deep multi-band imaging data produced by the Hyper Suprime-Cam (HSC) Subaru Strategic Program survey. The photometric candidates were selected by a Bayesian probabilistic algorithm, and then observed with spectrographs on the Gran Telescopio Canarias and the Subaru Telescope. Combined with the sample presented in the previous paper of this series, we have now identified 64 HSC sources over about 430 deg2, which include 33 high-z quasars, 14 high-z luminous galaxies, two [O III] emitters at z ˜ 0.8, and 15 Galactic brown dwarfs. The new quasars have considerably lower luminosity (M1450 ˜ -25 to -22 mag) than most of the previously known high-z quasars. Several of these quasars have luminous (>1043 erg s-1) and narrow (< 500 km s-1) Lyα lines, and also a possible mini broad-absorption-line system of N V λ1240 in the composite spectrum, which clearly separate them from typical quasars. On the other hand, the high-z galaxies have extremely high luminosities (M1450 ˜ -24 to -22 mag) compared to other galaxies found at similar redshifts. With the discovery of these new classes of objects, we are opening up new parameter spaces in the high-z Universe. Further survey observations and follow-up studies of the identified objects, including the construction of the quasar luminosity function at z ˜ 6, are ongoing.

  12. Electric field imaging of single atoms

    PubMed Central

    Shibata, Naoya; Seki, Takehito; Sánchez-Santolino, Gabriel; Findlay, Scott D.; Kohno, Yuji; Matsumoto, Takao; Ishikawa, Ryo; Ikuhara, Yuichi

    2017-01-01

    In scanning transmission electron microscopy (STEM), single atoms can be imaged by detecting electrons scattered through high angles using post-specimen, annular-type detectors. Recently, it has been shown that the atomic-scale electric field of both the positive atomic nuclei and the surrounding negative electrons within crystalline materials can be probed by atomic-resolution differential phase contrast STEM. Here we demonstrate the real-space imaging of the (projected) atomic electric field distribution inside single Au atoms, using sub-Å spatial resolution STEM combined with a high-speed segmented detector. We directly visualize that the electric field distribution (blurred by the sub-Å size electron probe) drastically changes within the single Au atom in a shape that relates to the spatial variation of total charge density within the atom. Atomic-resolution electric field mapping with single-atom sensitivity enables us to examine their detailed internal and boundary structures. PMID:28555629

  13. Material appearance acquisition from a single image

    NASA Astrophysics Data System (ADS)

    Zhang, Xu; Cui, Shulin; Cui, Hanwen; Yang, Lin; Wu, Tao

    2017-01-01

    The scope of this paper is to present a method of material appearance acquisition(MAA) from a single image. In this paper, material appearance is represented by spatially varying bidirectional reflectance distribution function(SVBRDF). Therefore, MAA can be reduced to the problem of recovery of each pixel's BRDF parameters from an original input image, which include diffuse coefficient, specular coefficient, normal and glossiness based on the Blinn-Phone model. In our method, the workflow of MAA includes five main phases: highlight removal, estimation of intrinsic images, shape from shading(SFS), initialization of glossiness and refining SVBRDF parameters based on IPOPT. The results indicate that the proposed technique can effectively extract the material appearance from a single image.

  14. A-Track: Detecting Moving Objects in FITS images

    NASA Astrophysics Data System (ADS)

    Atay, T.; Kaplan, M.; Kilic, Y.; Karapinar, N.

    2017-04-01

    A-Track is a fast, open-source, cross-platform pipeline for detecting moving objects (asteroids and comets) in sequential telescope images in FITS format. The moving objects are detected using a modified line detection algorithm.

  15. Multiple-Event, Single-Photon Counting Imaging Sensor

    NASA Technical Reports Server (NTRS)

    Zheng, Xinyu; Cunningham, Thomas J.; Sun, Chao; Wang, Kang L.

    2011-01-01

    The single-photon counting imaging sensor is typically an array of silicon Geiger-mode avalanche photodiodes that are monolithically integrated with CMOS (complementary metal oxide semiconductor) readout, signal processing, and addressing circuits located in each pixel and the peripheral area of the chip. The major problem is its single-event method for photon count number registration. A single-event single-photon counting imaging array only allows registration of up to one photon count in each of its pixels during a frame time, i.e., the interval between two successive pixel reset operations. Since the frame time can t be too short, this will lead to very low dynamic range and make the sensor merely useful for very low flux environments. The second problem of the prior technique is a limited fill factor resulting from consumption of chip area by the monolithically integrated CMOS readout in pixels. The resulting low photon collection efficiency will substantially ruin any benefit gained from the very sensitive single-photon counting detection. The single-photon counting imaging sensor developed in this work has a novel multiple-event architecture, which allows each of its pixels to register as more than one million (or more) photon-counting events during a frame time. Because of a consequently boosted dynamic range, the imaging array of the invention is capable of performing single-photon counting under ultra-low light through high-flux environments. On the other hand, since the multiple-event architecture is implemented in a hybrid structure, back-illumination and close-to-unity fill factor can be realized, and maximized quantum efficiency can also be achieved in the detector array.

  16. A model-based approach for detection of runways and other objects in image sequences acquired using an on-board camera

    NASA Technical Reports Server (NTRS)

    Kasturi, Rangachar; Devadiga, Sadashiva; Tang, Yuan-Liang

    1994-01-01

    This research was initiated as a part of the Advanced Sensor and Imaging System Technology (ASSIST) program at NASA Langley Research Center. The primary goal of this research is the development of image analysis algorithms for the detection of runways and other objects using an on-board camera. Initial effort was concentrated on images acquired using a passive millimeter wave (PMMW) sensor. The images obtained using PMMW sensors under poor visibility conditions due to atmospheric fog are characterized by very low spatial resolution but good image contrast compared to those images obtained using sensors operating in the visible spectrum. Algorithms developed for analyzing these images using a model of the runway and other objects are described in Part 1 of this report. Experimental verification of these algorithms was limited to a sequence of images simulated from a single frame of PMMW image. Subsequent development and evaluation of algorithms was done using video image sequences. These images have better spatial and temporal resolution compared to PMMW images. Algorithms for reliable recognition of runways and accurate estimation of spatial position of stationary objects on the ground have been developed and evaluated using several image sequences. These algorithms are described in Part 2 of this report. A list of all publications resulting from this work is also included.

  17. Reliability of a single objective measure in assessing sleepiness.

    PubMed

    Sunwoo, Bernie Y; Jackson, Nicholas; Maislin, Greg; Gurubhagavatula, Indira; George, Charles F; Pack, Allan I

    2012-01-01

    To evaluate reliability of single objective tests in assessing sleepiness. Subjects who completed polysomnography underwent a 4-nap multiple sleep latency test (MSLT) the following day. Prior to each nap opportunity on MSLT, subjects performed the psychomotor vigilance test (PVT) and divided attention driving task (DADT). Results of single versus multiple test administrations were compared using the intraclass correlation coefficient (ICC) and adjusted for test administration order effects to explore time of day effects. Measures were explored as continuous and binary (i.e., impaired or not impaired). Community-based sample evaluated at a tertiary, university-based sleep center. 372 adult commercial vehicle operators oversampled for increased obstructive sleep apnea risk. N/A. AS CONTINUOUS MEASURES, ICC WERE AS FOLLOWS: MSLT 0.45, PVT median response time 0.69, PVT number of lapses 0.51, 10-min DADT tracking error 0.87, 20-min DADT tracking error 0.90. Based on binary outcomes, ICC were: MSLT 0.63, PVT number of lapses 0.85, 10-min DADT 0.95, 20-min DADT 0.96. Statistically significant time of day effects were seen in both the MSLT and PVT but not the DADT. Correlation between ESS and different objective tests was strongest for MSLT, range [-0.270 to -0.195] and persisted across all time points. Single DADT and PVT administrations are reliable measures of sleepiness. A single MSLT administration can reasonably discriminate individuals with MSL < 8 minutes. These results support the use of a single administration of some objective tests of sleepiness when performed under controlled conditions in routine clinical care.

  18. Single-frequency 3D synthetic aperture imaging with dynamic metasurface antennas.

    PubMed

    Boyarsky, Michael; Sleasman, Timothy; Pulido-Mancera, Laura; Diebold, Aaron V; Imani, Mohammadreza F; Smith, David R

    2018-05-20

    Through aperture synthesis, an electrically small antenna can be used to form a high-resolution imaging system capable of reconstructing three-dimensional (3D) scenes. However, the large spectral bandwidth typically required in synthetic aperture radar systems to resolve objects in range often requires costly and complex RF components. We present here an alternative approach based on a hybrid imaging system that combines a dynamically reconfigurable aperture with synthetic aperture techniques, demonstrating the capability to resolve objects in three dimensions (3D), with measurements taken at a single frequency. At the core of our imaging system are two metasurface apertures, both of which consist of a linear array of metamaterial irises that couple to a common waveguide feed. Each metamaterial iris has integrated within it a diode that can be biased so as to switch the element on (radiating) or off (non-radiating), such that the metasurface antenna can produce distinct radiation profiles corresponding to different on/off patterns of the metamaterial element array. The electrically large size of the metasurface apertures enables resolution in range and one cross-range dimension, while aperture synthesis provides resolution in the other cross-range dimension. The demonstrated imaging capabilities of this system represent a step forward in the development of low-cost, high-performance 3D microwave imaging systems.

  19. Readout-Segmented Echo-Planar Imaging in Diffusion-Weighted MR Imaging in Breast Cancer: Comparison with Single-Shot Echo-Planar Imaging in Image Quality

    PubMed Central

    Kim, Yun Ju; Kang, Bong Joo; Park, Chang Suk; Kim, Hyeon Sook; Son, Yo Han; Porter, David Andrew; Song, Byung Joo

    2014-01-01

    Objective The purpose of this study was to compare the image quality of standard single-shot echo-planar imaging (ss-EPI) and that of readout-segmented EPI (rs-EPI) in patients with breast cancer. Materials and Methods Seventy-one patients with 74 breast cancers underwent both ss-EPI and rs-EPI. For qualitative comparison of image quality, three readers independently assessed the two sets of diffusion-weighted (DW) images. To evaluate geometric distortion, a comparison was made between lesion lengths derived from contrast enhanced MR (CE-MR) images and those obtained from the corresponding DW images. For assessment of image parameters, signal-to-noise ratio (SNR), lesion contrast, and contrast-to-noise ratio (CNR) were calculated. Results The rs-EPI was superior to ss-EPI in most criteria regarding the qualitative image quality. Anatomical structure distinction, delineation of the lesion, ghosting artifact, and overall image quality were significantly better in rs-EPI. Regarding the geometric distortion, lesion length on ss-EPI was significantly different from that of CE-MR, whereas there were no significant differences between CE-MR and rs-EPI. The rs-EPI was superior to ss-EPI in SNR and CNR. Conclusion Readout-segmented EPI is superior to ss-EPI in the aspect of image quality in DW MR imaging of the breast. PMID:25053898

  20. Object-oriented recognition of high-resolution remote sensing image

    NASA Astrophysics Data System (ADS)

    Wang, Yongyan; Li, Haitao; Chen, Hong; Xu, Yuannan

    2016-01-01

    With the development of remote sensing imaging technology and the improvement of multi-source image's resolution in satellite visible light, multi-spectral and hyper spectral , the high resolution remote sensing image has been widely used in various fields, for example military field, surveying and mapping, geophysical prospecting, environment and so forth. In remote sensing image, the segmentation of ground targets, feature extraction and the technology of automatic recognition are the hotspot and difficulty in the research of modern information technology. This paper also presents an object-oriented remote sensing image scene classification method. The method is consist of vehicles typical objects classification generation, nonparametric density estimation theory, mean shift segmentation theory, multi-scale corner detection algorithm, local shape matching algorithm based on template. Remote sensing vehicles image classification software system is designed and implemented to meet the requirements .

  1. A Perceptually Weighted Rank Correlation Indicator for Objective Image Quality Assessment

    NASA Astrophysics Data System (ADS)

    Wu, Qingbo; Li, Hongliang; Meng, Fanman; Ngan, King N.

    2018-05-01

    In the field of objective image quality assessment (IQA), the Spearman's $\\rho$ and Kendall's $\\tau$ are two most popular rank correlation indicators, which straightforwardly assign uniform weight to all quality levels and assume each pair of images are sortable. They are successful for measuring the average accuracy of an IQA metric in ranking multiple processed images. However, two important perceptual properties are ignored by them as well. Firstly, the sorting accuracy (SA) of high quality images are usually more important than the poor quality ones in many real world applications, where only the top-ranked images would be pushed to the users. Secondly, due to the subjective uncertainty in making judgement, two perceptually similar images are usually hardly sortable, whose ranks do not contribute to the evaluation of an IQA metric. To more accurately compare different IQA algorithms, we explore a perceptually weighted rank correlation indicator in this paper, which rewards the capability of correctly ranking high quality images, and suppresses the attention towards insensitive rank mistakes. More specifically, we focus on activating `valid' pairwise comparison towards image quality, whose difference exceeds a given sensory threshold (ST). Meanwhile, each image pair is assigned an unique weight, which is determined by both the quality level and rank deviation. By modifying the perception threshold, we can illustrate the sorting accuracy with a more sophisticated SA-ST curve, rather than a single rank correlation coefficient. The proposed indicator offers a new insight for interpreting visual perception behaviors. Furthermore, the applicability of our indicator is validated in recommending robust IQA metrics for both the degraded and enhanced image data.

  2. Image formation of thick three-dimensional objects in differential-interference-contrast microscopy.

    PubMed

    Trattner, Sigal; Kashdan, Eugene; Feigin, Micha; Sochen, Nir

    2014-05-01

    The differential-interference-contrast (DIC) microscope is of widespread use in life sciences as it enables noninvasive visualization of transparent objects. The goal of this work is to model the image formation process of thick three-dimensional objects in DIC microscopy. The model is based on the principles of electromagnetic wave propagation and scattering. It simulates light propagation through the components of the DIC microscope to the image plane using a combined geometrical and physical optics approach and replicates the DIC image of the illuminated object. The model is evaluated by comparing simulated images of three-dimensional spherical objects with the recorded images of polystyrene microspheres. Our computer simulations confirm that the model captures the major DIC image characteristics of the simulated object, and it is sensitive to the defocusing effects.

  3. Object localization in handheld thermal images for fireground understanding

    NASA Astrophysics Data System (ADS)

    Vandecasteele, Florian; Merci, Bart; Jalalvand, Azarakhsh; Verstockt, Steven

    2017-05-01

    Despite the broad application of the handheld thermal imaging cameras in firefighting, its usage is mostly limited to subjective interpretation by the person carrying the device. As remedies to overcome this limitation, object localization and classification mechanisms could assist the fireground understanding and help with the automated localization, characterization and spatio-temporal (spreading) analysis of the fire. An automated understanding of thermal images can enrich the conventional knowledge-based firefighting techniques by providing the information from the data and sensing-driven approaches. In this work, transfer learning is applied on multi-labeling convolutional neural network architectures for object localization and recognition in monocular visual, infrared and multispectral dynamic images. Furthermore, the possibility of analyzing fire scene images is studied and their current limitations are discussed. Finally, the understanding of the room configuration (i.e., objects location) for indoor localization in reduced visibility environments and the linking with Building Information Models (BIM) are investigated.

  4. Effects of Implied Motion and Facing Direction on Positional Preferences in Single-Object Pictures.

    PubMed

    Palmer, Stephen E; Langlois, Thomas A

    2017-07-01

    Palmer, Gardner, and Wickens studied aesthetic preferences for pictures of single objects and found a strong inward bias: Right-facing objects were preferred left-of-center and left-facing objects right-of-center. They found no effect of object motion (people and cars showed the same inward bias as chairs and teapots), but the objects were not depicted as moving. Here we measured analogous inward biases with objects depicted as moving with an implied direction and speed by having participants drag-and-drop target objects into the most aesthetically pleasing position. In Experiment 1, human figures were shown diving or falling while moving forward or backward. Aesthetic biases were evident for both inward-facing and inward-moving figures, but the motion-based bias dominated so strongly that backward divers or fallers were preferred moving inward but facing outward. Experiment 2 investigated implied speed effects using images of humans, horses, and cars moving at different speeds (e.g., standing, walking, trotting, and galloping horses). Inward motion or facing biases were again present, and differences in their magnitude due to speed were evident. Unexpectedly, faster moving objects were generally preferred closer to frame center than slower moving objects. These results are discussed in terms of the combined effects of prospective, future-oriented biases, and retrospective, past-oriented biases.

  5. Systems and methods for detecting an image of an object by use of an X-ray beam having a polychromatic distribution

    DOEpatents

    Parham, Christopher; Zhong, Zhong; Pisano, Etta; Connor, Dean; Chapman, Leroy D.

    2010-06-22

    Systems and methods for detecting an image of an object using an X-ray beam having a polychromatic energy distribution are disclosed. According to one aspect, a method can include detecting an image of an object. The method can include generating a first X-ray beam having a polychromatic energy distribution. Further, the method can include positioning a single monochromator crystal in a predetermined position to directly intercept the first X-ray beam such that a second X-ray beam having a predetermined energy level is produced. Further, an object can be positioned in the path of the second X-ray beam for transmission of the second X-ray beam through the object and emission from the object as a transmitted X-ray beam. The transmitted X-ray beam can be directed at an angle of incidence upon a crystal analyzer. Further, an image of the object can be detected from a beam diffracted from the analyzer crystal.

  6. Disease quantification on PET/CT images without object delineation

    NASA Astrophysics Data System (ADS)

    Tong, Yubing; Udupa, Jayaram K.; Odhner, Dewey; Wu, Caiyun; Fitzpatrick, Danielle; Winchell, Nicole; Schuster, Stephen J.; Torigian, Drew A.

    2017-03-01

    The derivation of quantitative information from images to make quantitative radiology (QR) clinically practical continues to face a major image analysis hurdle because of image segmentation challenges. This paper presents a novel approach to disease quantification (DQ) via positron emission tomography/computed tomography (PET/CT) images that explores how to decouple DQ methods from explicit dependence on object segmentation through the use of only object recognition results to quantify disease burden. The concept of an object-dependent disease map is introduced to express disease severity without performing explicit delineation and partial volume correction of either objects or lesions. The parameters of the disease map are estimated from a set of training image data sets. The idea is illustrated on 20 lung lesions and 20 liver lesions derived from 18F-2-fluoro-2-deoxy-D-glucose (FDG)-PET/CT scans of patients with various types of cancers and also on 20 NEMA PET/CT phantom data sets. Our preliminary results show that, on phantom data sets, "disease burden" can be estimated to within 2% of known absolute true activity. Notwithstanding the difficulty in establishing true quantification on patient PET images, our results achieve 8% deviation from "true" estimates, with slightly larger deviations for small and diffuse lesions where establishing ground truth becomes really questionable, and smaller deviations for larger lesions where ground truth set up becomes more reliable. We are currently exploring extensions of the approach to include fully automated body-wide DQ, extensions to just CT or magnetic resonance imaging (MRI) alone, to PET/CT performed with radiotracers other than FDG, and other functional forms of disease maps.

  7. A single-chain variable fragment intrabody prevents intracellular polymerization of Z α1-antitrypsin while allowing its antiproteinase activity

    PubMed Central

    Ordóñez, Adriana; Pérez, Juan; Tan, Lu; Dickens, Jennifer A.; Motamedi-Shad, Neda; Irving, James A.; Haq, Imran; Ekeowa, Ugo; Marciniak, Stefan J.; Miranda, Elena; Lomas, David A.

    2015-01-01

    Mutant Z α1-antitrypsin (E342K) accumulates as polymers within the endoplasmic reticulum (ER) of hepatocytes predisposing to liver disease, whereas low levels of circulating Z α1-antitrypsin lead to emphysema by loss of inhibition of neutrophil elastase. The ideal therapy should prevent polymer formation while preserving inhibitory activity. Here we used mAb technology to identify interactors with Z α1-antitrypsin that comply with both requirements. We report the generation of an mAb (4B12) that blocked α1-antitrypsin polymerization in vitro at a 1:1 molar ratio, causing a small increase of the stoichiometry of inhibition for neutrophil elastase. A single-chain variable fragment (scFv) intrabody was generated based on the sequence of mAb4B12. The expression of scFv4B12 within the ER (scFv4B12KDEL) and along the secretory pathway (scFv4B12) reduced the intracellular polymerization of Z α1-antitrypsin by 60%. The scFv4B12 intrabody also increased the secretion of Z α1-antitrypsin that retained inhibitory activity against neutrophil elastase. MAb4B12 recognized a discontinuous epitope probably located in the region of helices A/C/G/H/I and seems to act by altering protein dynamics rather than binding preferentially to the native state. This novel approach could reveal new target sites for small-molecule intervention that may block the transition to aberrant polymers without compromising the inhibitory activity of Z α1-antitrypsin.—Ordóñez, A., Pérez, J., Tan, L., Dickens, J. A., Motamedi-Shad, N., Irving, J. A., Haq, I., Ekeowa, U., Marciniak, S. J., Miranda, E., Lomas, D. A. A single-chain variable fragment intrabody prevents intracellular polymerization of Z α1-antitrypsin while allowing its antiproteinase activity. PMID:25757566

  8. Accurate modelling of single-particle cryo-EM images quantifies the benefits expected from using Zernike phase contrast

    PubMed Central

    Hall, R. J.; Nogales, E.; Glaeser, R. M.

    2011-01-01

    The use of a Zernike-type phase plate in biological cryo-electron microscopy allows the imaging, without using defocus, of what are predominantly phase objects. It is thought that such phase-plate implementations might result in higher quality images, free from the problems of CTF correction that occur when images must be recorded at extremely high values of defocus. In single-particle cryo-electron microscopy it is hoped that these improvements in image quality will facilitate work on structures that have proved difficult to study, either because of their relatively small size or because the structures are not completely homogeneous. There is still a need, however, to quantify how much improvement can be gained by using a phase plate for single-particle cryo-electron microscopy. We present a method for quantitatively modelling the images recorded with 200 keV electrons, for single particles embedded in vitreous ice. We then investigate what difference the use of a phase-plate device could have on the processing of single-particle data. We confirm that using a phase plate results in single-particle datasets in which smaller molecules can be detected, particles can be more accurately aligned and problems of heterogeneity can be more easily addressed. PMID:21463690

  9. Spatially resolved single crystal x-ray spectropolarimetry of wire array z-pinch plasmas

    NASA Astrophysics Data System (ADS)

    Wallace, M. S.; Haque, S.; Neill, P.; Pereira, N. R.; Presura, R.

    2018-01-01

    A recently developed single-crystal x-ray spectropolarimeter has been used to record paired sets of polarization-dependent and axially resolved x-ray spectra emitted by wire array z-pinches. In this measurement, two internal planes inside a suitable crystal diffract the x-rays into two perpendicular directions that are normal to each other, thereby separating incident x-rays into their linearly polarized components. This paper gives considerations for fielding the instrument on extended sources. Results from extended sources are difficult to interpret because generally the incident x-rays are not separated properly by the crystal. This difficulty is mitigated by using a series of collimating slits to select incident x-rays that propagate in a plane of symmetry between the polarization-splitting planes. The resulting instrument and some of the spatially resolved polarized x-ray spectra recorded for a 1-MA aluminum wire array z-pinch at the Nevada Terawatt Facility at the University of Nevada, Reno will be presented.

  10. Spatially resolved single crystal x-ray spectropolarimetry of wire array z-pinch plasmas.

    PubMed

    Wallace, M S; Haque, S; Neill, P; Pereira, N R; Presura, R

    2018-01-01

    A recently developed single-crystal x-ray spectropolarimeter has been used to record paired sets of polarization-dependent and axially resolved x-ray spectra emitted by wire array z-pinches. In this measurement, two internal planes inside a suitable crystal diffract the x-rays into two perpendicular directions that are normal to each other, thereby separating incident x-rays into their linearly polarized components. This paper gives considerations for fielding the instrument on extended sources. Results from extended sources are difficult to interpret because generally the incident x-rays are not separated properly by the crystal. This difficulty is mitigated by using a series of collimating slits to select incident x-rays that propagate in a plane of symmetry between the polarization-splitting planes. The resulting instrument and some of the spatially resolved polarized x-ray spectra recorded for a 1-MA aluminum wire array z-pinch at the Nevada Terawatt Facility at the University of Nevada, Reno will be presented.

  11. Small Field-of-view single-shot EPI-DWI of the prostate: Evaluation of spatially-tailored two-dimensional radiofrequency excitation pulses.

    PubMed

    Attenberger, Ulrike I; Rathmann, Nils; Sertdemir, Metin; Riffel, Philipp; Weidner, Anja; Kannengiesser, Stefan; Morelli, John N; Schoenberg, Stefan O; Hausmann, Daniel

    2016-06-01

    Spatially-tailored (RF) excitation pulses in echo-planar imaging (EPI), combined with a decreased FOV in the phase-encoding direction, enable a reduction of k-space acquisition lines, which shortens the echo train length (ETL) and reduces susceptibility artifacts. The purpose of this study was to evaluate the image quality of a zoomed EPI (z-EPI) sequence in diffusion-weighted imaging (DWI) of the prostate in comparison to a conventional single-shot EPI using single-channel (c-EPI1) and multi-channel (c-EPI2) RF excitation, with and without use of an endorectal coil. 33 consecutive patients (mean age: 61 +/- 9 years; mean PSA: 8.67±6.23 ng/ml) with examinations between 10/2012 and 02/2014 were analyzed in this retrospective study. In 26 of 33 patients the initial multiparametric (mp)-MRI was performed on a whole-body 3T scanner (Magnetom Trio, Siemens, Erlangen, Germany) using an endorectal coil (c (conventional)-EPI1). Zoomed-EPI (Z-EPI) examinations of these patients and a complete mp-MRI protocol including c-EPI2 of 7 additional patients were carried out on another 3T wb MR scanner with two-channel dynamic parallel transmit capability (Magnetom Skyra with TimTX TrueShape, Siemens). For z-EPI, the one-dimensional spatially selective RF excitation pulse was replaced by a two-dimensional RF pulse. Degree of image blur and susceptibility artifacts (0=not present to 3= non-diagnostic), maximum image distortion (mm), apparent diffusion coefficient (ADC) values, as well as overall scan preference were evaluated. SNR maps were generated to compare c-EPI2 and z-EPI. Overall image quality of z-EPI was preferred by both readers in all examinations with a single exception. Susceptibility artifacts were rated significantly lower on z-EPI compared to both other methods (z-EPI vs c-EPI1: p<0.01; z-EPI vs c-EPI2: p<0.01) as well as image blur (z-EPI vs c-EPI1: p<0.01; z-EPI vs c-EPI2: p<0.01). Image distortion was not statistically significantly reduced with z-EPI (z-EPI vs c

  12. Evidence of Primordial Clustering around the QSO SDSS J1030+0524 at z=6.28

    NASA Astrophysics Data System (ADS)

    Stiavelli, M.; Djorgovski, S. G.; Pavlovsky, C.; Scarlata, C.; Stern, D.; Mahabal, A.; Thompson, D.; Dickinson, M.; Panagia, N.; Meylan, G.

    2005-03-01

    We present tentative evidence of primordial clustering, manifested as an excess of color-selected objects in the field of the QSO SDSS J1030+0524 at redshift z=6.28. We have selected objects red in i775-z850 on the basis of Hubble Space Telescope Advanced Camera for Surveys imaging of a field centered on the QSO. Compared to data at comparable depth obtained by the Great Observatories Origins Deep Survey, we find an excess of objects with i775-z850>=1.5 in the QSO field. The significance of the detection is estimated to be ~97% on the basis of the counts alone and increases to 99.4% if one takes into account the color distribution. If confirmed, this would represent the highest redshift example of galaxy clustering and would have implications on models for the growth of structure. Bias-driven clustering of first luminous objects forming in the highest peaks of the primordial density field is expected in most models of early structure formation. The redshift of one of the candidates has been found to be z=5.970 by our spectroscopy with the Keck I Low Resolution Imaging Spectrometer, confirming the validity of our color selection. Based, in part, on data obtained at the W. M. Keck Observatory, which is operated as a scientific partnership between the California Institute of Technology, the University of California, and NASA, and was made possible by the generous financial support of the W. M. Keck Foundation.

  13. Single Lens Dual-Aperture 3D Imaging System: Color Modeling

    NASA Technical Reports Server (NTRS)

    Bae, Sam Y.; Korniski, Ronald; Ream, Allen; Fritz, Eric; Shearn, Michael

    2012-01-01

    In an effort to miniaturize a 3D imaging system, we created two viewpoints in a single objective lens camera. This was accomplished by placing a pair of Complementary Multi-band Bandpass Filters (CMBFs) in the aperture area. Two key characteristics about the CMBFs are that the passbands are staggered so only one viewpoint is opened at a time when a light band matched to that passband is illuminated, and the passbands are positioned throughout the visible spectrum, so each viewpoint can render color by taking RGB spectral images. Each viewpoint takes a different spectral image from the other viewpoint hence yielding a different color image relative to the other. This color mismatch in the two viewpoints could lead to color rivalry, where the human vision system fails to resolve two different colors. The difference will be closer if the number of passbands in a CMBF increases. (However, the number of passbands is constrained by cost and fabrication technique.) In this paper, simulation predicting the color mismatch is reported.

  14. Analysis and segmentation of images in case of solving problems of detecting and tracing objects on real-time video

    NASA Astrophysics Data System (ADS)

    Ezhova, Kseniia; Fedorenko, Dmitriy; Chuhlamov, Anton

    2016-04-01

    The article deals with the methods of image segmentation based on color space conversion, and allow the most efficient way to carry out the detection of a single color in a complex background and lighting, as well as detection of objects on a homogeneous background. The results of the analysis of segmentation algorithms of this type, the possibility of their implementation for creating software. The implemented algorithm is very time-consuming counting, making it a limited application for the analysis of the video, however, it allows us to solve the problem of analysis of objects in the image if there is no dictionary of images and knowledge bases, as well as the problem of choosing the optimal parameters of the frame quantization for video analysis.

  15. Full-frame, high-speed 3D shape and deformation measurements using stereo-digital image correlation and a single color high-speed camera

    NASA Astrophysics Data System (ADS)

    Yu, Liping; Pan, Bing

    2017-08-01

    Full-frame, high-speed 3D shape and deformation measurement using stereo-digital image correlation (stereo-DIC) technique and a single high-speed color camera is proposed. With the aid of a skillfully designed pseudo stereo-imaging apparatus, color images of a test object surface, composed of blue and red channel images from two different optical paths, are recorded by a high-speed color CMOS camera. The recorded color images can be separated into red and blue channel sub-images using a simple but effective color crosstalk correction method. These separated blue and red channel sub-images are processed by regular stereo-DIC method to retrieve full-field 3D shape and deformation on the test object surface. Compared with existing two-camera high-speed stereo-DIC or four-mirror-adapter-assisted singe-camera high-speed stereo-DIC, the proposed single-camera high-speed stereo-DIC technique offers prominent advantages of full-frame measurements using a single high-speed camera but without sacrificing its spatial resolution. Two real experiments, including shape measurement of a curved surface and vibration measurement of a Chinese double-side drum, demonstrated the effectiveness and accuracy of the proposed technique.

  16. Anthropomorphic robot for recognition and drawing generalized object images

    NASA Astrophysics Data System (ADS)

    Ginzburg, Vera M.

    1998-10-01

    The process of recognition, for instance, understanding the text, written by different fonts, consists in the depriving of the individual attributes of the letters in the particular font. It is shown that such process, in Nature and technique, can be provided by the narrowing the spatial frequency of the object's image by its defocusing. In defocusing images remain only areas, so-called Informative Fragments (IFs), which all together form the generalized (stylized) image of many identical objects. It is shown that the variety of shapes of IFs is restricted and can be presented by `Geometrical alphabet'. The `letters' for this alphabet can be created using two basic `genetic' figures: a stripe and round spot. It is known from physiology that the special cells of visual cortex response to these particular figures. The prototype of such `genetic' alphabet has been made using Boolean algebra (Venn's diagrams). The algorithm for drawing the letter's (`genlet's') shape in this alphabet and generalized images of objects (for example, `sleeping cat'), are given. A scheme of an anthropomorphic robot is shown together with results of model computer experiment of the robot's action--`drawing' the generalized image.

  17. Method and apparatus for enhanced sensitivity filmless medical x-ray imaging, including three-dimensional imaging

    DOEpatents

    Parker, S.

    1995-10-24

    A filmless X-ray imaging system includes at least one X-ray source, upper and lower collimators, and a solid-state detector array, and can provide three-dimensional imaging capability. The X-ray source plane is distance z{sub 1} above upper collimator plane, distance z{sub 2} above the lower collimator plane, and distance z{sub 3} above the plane of the detector array. The object to be X-rayed is located between the upper and lower collimator planes. The upper and lower collimators and the detector array are moved horizontally with scanning velocities v{sub 1}, v{sub 2}, v{sub 3} proportional to z{sub 1}, z{sub 2} and z{sub 3}, respectively. The pattern and size of openings in the collimators, and between detector positions is proportional such that similar triangles are always defined relative to the location of the X-ray source. X-rays that pass through openings in the upper collimator will always pass through corresponding and similar openings in the lower collimator, and thence to a corresponding detector in the underlying detector array. Substantially 100% of the X-rays irradiating the object (and neither absorbed nor scattered) pass through the lower collimator openings and are detected, which promotes enhanced sensitivity. A computer system coordinates repositioning of the collimators and detector array, and X-ray source locations. The computer system can store detector array output, and can associate a known X-ray source location with detector array output data, to provide three-dimensional imaging. Detector output may be viewed instantly, stored digitally, and/or transmitted electronically for image viewing at a remote site. 5 figs.

  18. Method and apparatus for enhanced sensitivity filmless medical x-ray imaging, including three-dimensional imaging

    DOEpatents

    Parker, Sherwood

    1995-01-01

    A filmless X-ray imaging system includes at least one X-ray source, upper and lower collimators, and a solid-state detector array, and can provide three-dimensional imaging capability. The X-ray source plane is distance z.sub.1 above upper collimator plane, distance z.sub.2 above the lower collimator plane, and distance z.sub.3 above the plane of the detector array. The object to be X-rayed is located between the upper and lower collimator planes. The upper and lower collimators and the detector array are moved horizontally with scanning velocities v.sub.1, v.sub.2, v.sub.3 proportional to z.sub.1, z.sub.2 and z.sub.3, respectively. The pattern and size of openings in the collimators, and between detector positions is proportional such that similar triangles are always defined relative to the location of the X-ray source. X-rays that pass through openings in the upper collimator will always pass through corresponding and similar openings in the lower collimator, and thence to a corresponding detector in the underlying detector array. Substantially 100% of the X-rays irradiating the object (and neither absorbed nor scattered) pass through the lower collimator openings and are detected, which promotes enhanced sensitivity. A computer system coordinates repositioning of the collimators and detector array, and X-ray source locations. The computer system can store detector array output, and can associate a known X-ray source location with detector array output data, to provide three-dimensional imaging. Detector output may be viewed instantly, stored digitally, and/or transmitted electronically for image viewing at a remote site.

  19. Optical computed tomography for spatially isotropic four-dimensional imaging of live single cells

    PubMed Central

    Kelbauskas, Laimonas; Shetty, Rishabh; Cao, Bin; Wang, Kuo-Chen; Smith, Dean; Wang, Hong; Chao, Shi-Hui; Gangaraju, Sandhya; Ashcroft, Brian; Kritzer, Margaret; Glenn, Honor; Johnson, Roger H.; Meldrum, Deirdre R.

    2017-01-01

    Quantitative three-dimensional (3D) computed tomography (CT) imaging of living single cells enables orientation-independent morphometric analysis of the intricacies of cellular physiology. Since its invention, x-ray CT has become indispensable in the clinic for diagnostic and prognostic purposes due to its quantitative absorption-based imaging in true 3D that allows objects of interest to be viewed and measured from any orientation. However, x-ray CT has not been useful at the level of single cells because there is insufficient contrast to form an image. Recently, optical CT has been developed successfully for fixed cells, but this technology called Cell-CT is incompatible with live-cell imaging due to the use of stains, such as hematoxylin, that are not compatible with cell viability. We present a novel development of optical CT for quantitative, multispectral functional 4D (three spatial + one spectral dimension) imaging of living single cells. The method applied to immune system cells offers truly isotropic 3D spatial resolution and enables time-resolved imaging studies of cells suspended in aqueous medium. Using live-cell optical CT, we found a heterogeneous response to mitochondrial fission inhibition in mouse macrophages and differential basal remodeling of small (0.1 to 1 fl) and large (1 to 20 fl) nuclear and mitochondrial structures on a 20- to 30-s time scale in human myelogenous leukemia cells. Because of its robust 3D measurement capabilities, live-cell optical CT represents a powerful new tool in the biomedical research field. PMID:29226240

  20. Feedforward object-vision models only tolerate small image variations compared to human

    PubMed Central

    Ghodrati, Masoud; Farzmahdi, Amirhossein; Rajaei, Karim; Ebrahimpour, Reza; Khaligh-Razavi, Seyed-Mahdi

    2014-01-01

    Invariant object recognition is a remarkable ability of primates' visual system that its underlying mechanism has constantly been under intense investigations. Computational modeling is a valuable tool toward understanding the processes involved in invariant object recognition. Although recent computational models have shown outstanding performances on challenging image databases, they fail to perform well in image categorization under more complex image variations. Studies have shown that making sparse representation of objects by extracting more informative visual features through a feedforward sweep can lead to higher recognition performances. Here, however, we show that when the complexity of image variations is high, even this approach results in poor performance compared to humans. To assess the performance of models and humans in invariant object recognition tasks, we built a parametrically controlled image database consisting of several object categories varied in different dimensions and levels, rendered from 3D planes. Comparing the performance of several object recognition models with human observers shows that only in low-level image variations the models perform similar to humans in categorization tasks. Furthermore, the results of our behavioral experiments demonstrate that, even under difficult experimental conditions (i.e., briefly presented masked stimuli with complex image variations), human observers performed outstandingly well, suggesting that the models are still far from resembling humans in invariant object recognition. Taken together, we suggest that learning sparse informative visual features, although desirable, is not a complete solution for future progresses in object-vision modeling. We show that this approach is not of significant help in solving the computational crux of object recognition (i.e., invariant object recognition) when the identity-preserving image variations become more complex. PMID:25100986

  1. Single-Cell Analysis Using Hyperspectral Imaging Modalities.

    PubMed

    Mehta, Nishir; Shaik, Shahensha; Devireddy, Ram; Gartia, Manas Ranjan

    2018-02-01

    Almost a decade ago, hyperspectral imaging (HSI) was employed by the NASA in satellite imaging applications such as remote sensing technology. This technology has since been extensively used in the exploration of minerals, agricultural purposes, water resources, and urban development needs. Due to recent advancements in optical re-construction and imaging, HSI can now be applied down to micro- and nanometer scales possibly allowing for exquisite control and analysis of single cell to complex biological systems. This short review provides a description of the working principle of the HSI technology and how HSI can be used to assist, substitute, and validate traditional imaging technologies. This is followed by a description of the use of HSI for biological analysis and medical diagnostics with emphasis on single-cell analysis using HSI.

  2. Dual-modal three-dimensional imaging of single cells with isometric high resolution using an optical projection tomography microscope

    NASA Astrophysics Data System (ADS)

    Miao, Qin; Rahn, J. Richard; Tourovskaia, Anna; Meyer, Michael G.; Neumann, Thomas; Nelson, Alan C.; Seibel, Eric J.

    2009-11-01

    The practice of clinical cytology relies on bright-field microscopy using absorption dyes like hematoxylin and eosin in the transmission mode, while the practice of research microscopy relies on fluorescence microscopy in the epi-illumination mode. The optical projection tomography microscope is an optical microscope that can generate 3-D images of single cells with isometric high resolution both in absorption and fluorescence mode. Although the depth of field of the microscope objective is in the submicron range, it can be extended by scanning the objective's focal plane. The extended depth of field image is similar to a projection in a conventional x-ray computed tomography. Cells suspended in optical gel flow through a custom-designed microcapillary. Multiple pseudoprojection images are taken by rotating the microcapillary. After these pseudoprojection images are further aligned, computed tomography methods are applied to create 3-D reconstruction. 3-D reconstructed images of single cells are shown in both absorption and fluorescence mode. Fluorescence spatial resolution is measured at 0.35 μm in both axial and lateral dimensions. Since fluorescence and absorption images are taken in two different rotations, mechanical error may cause misalignment of 3-D images. This mechanical error is estimated to be within the resolution of the system.

  3. The effect of input data transformations on object-based image analysis

    PubMed Central

    LIPPITT, CHRISTOPHER D.; COULTER, LLOYD L.; FREEMAN, MARY; LAMANTIA-BISHOP, JEFFREY; PANG, WYSON; STOW, DOUGLAS A.

    2011-01-01

    The effect of using spectral transform images as input data on segmentation quality and its potential effect on products generated by object-based image analysis are explored in the context of land cover classification in Accra, Ghana. Five image data transformations are compared to untransformed spectral bands in terms of their effect on segmentation quality and final product accuracy. The relationship between segmentation quality and product accuracy is also briefly explored. Results suggest that input data transformations can aid in the delineation of landscape objects by image segmentation, but the effect is idiosyncratic to the transformation and object of interest. PMID:21673829

  4. The evaluation of single-view and multi-view fusion 3D echocardiography using image-driven segmentation and tracking.

    PubMed

    Rajpoot, Kashif; Grau, Vicente; Noble, J Alison; Becher, Harald; Szmigielski, Cezary

    2011-08-01

    Real-time 3D echocardiography (RT3DE) promises a more objective and complete cardiac functional analysis by dynamic 3D image acquisition. Despite several efforts towards automation of left ventricle (LV) segmentation and tracking, these remain challenging research problems due to the poor-quality nature of acquired images usually containing missing anatomical information, speckle noise, and limited field-of-view (FOV). Recently, multi-view fusion 3D echocardiography has been introduced as acquiring multiple conventional single-view RT3DE images with small probe movements and fusing them together after alignment. This concept of multi-view fusion helps to improve image quality and anatomical information and extends the FOV. We now take this work further by comparing single-view and multi-view fused images in a systematic study. In order to better illustrate the differences, this work evaluates image quality and information content of single-view and multi-view fused images using image-driven LV endocardial segmentation and tracking. The image-driven methods were utilized to fully exploit image quality and anatomical information present in the image, thus purposely not including any high-level constraints like prior shape or motion knowledge in the analysis approaches. Experiments show that multi-view fused images are better suited for LV segmentation and tracking, while relatively more failures and errors were observed on single-view images. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. Imaging the host galaxies of high-redshift radio-quiet QSOs

    NASA Technical Reports Server (NTRS)

    Lowenthal, James D.; Heckman, Timothy M.; Lehnert, Matthew, D.; Elias, J. H.

    1995-01-01

    We present new deep K-band and optical images of four radio-quiet QSOs at z approximately = 1 and six radio-quiet QSOs at z approximately = 2.5, as well as optical images only of six more at z approximately = 2.5. We have examined the images carefully for evidence of extended 'fuzz' from any putative QSO host galaxy. None of the z approximately = 2.5 QSOs shows any extended emission, and only two of the z approximately = 1 QSOs show marginal evidence for extended emission. Our 3 sigma detection limits in the K images, m(sub K) approximately = 21 for an isolated source, would correspond approximately to an unevolved L(sup star) elliptical galaxy at z = 2.5 or 2-3 mag fainter than an L(sup star) elliptical at z = 1, although our limits on host galaxy light are weaker than this due to the difficulty of separating galaxy light from QSO light. We simulate simple models of disk and elliptical host galaxies, and find that the marginal emission around the two z approximately = 1 QSOs can be explained by disks or bulges that are approximately 1-2 mag brighter than an unevolved L(sup star) galaxy in one case and approximately 1.5-2.5 mag brighter than L(sub star) in the other. For two other z approximately = 1 QSOs, we have only upper limits (L approximately = L(sup star)). The hosts of the high-redshift sample must be no brighter than about 3 mag above an unevolved L(sup star) galaxy, and are at least 1 magnitude fainter than the hosts of radio-loud QSOs at the same redshift. If the easily detected K-band light surrounding a previous sample of otherwise similar but radio-loud QSOs is starlight, then it must evolve on timescales of greater than or approximately equal to 10(exp 8) yr (e.g., Chambers & Charlot 1990); therefore our non-detection of host galaxy fuzz around radio-quiet QSOs supports the view that high-redshift radio-quiet and radio-loud QSOs inhabit different host objects, rather than being single types of objects that turn their radio emission on and off over

  6. Architectures for single-chip image computing

    NASA Astrophysics Data System (ADS)

    Gove, Robert J.

    1992-04-01

    This paper will focus on the architectures of VLSI programmable processing components for image computing applications. TI, the maker of industry-leading RISC, DSP, and graphics components, has developed an architecture for a new-generation of image processors capable of implementing a plurality of image, graphics, video, and audio computing functions. We will show that the use of a single-chip heterogeneous MIMD parallel architecture best suits this class of processors--those which will dominate the desktop multimedia, document imaging, computer graphics, and visualization systems of this decade.

  7. An infrared modular panoramic imaging objective

    NASA Astrophysics Data System (ADS)

    Palmer, Troy A.; Alexay, Christopher C.

    2004-08-01

    We describe the optical and mechanical design of an athermal infrared objective lens with an afocal anamorphic adapter. The lens presented consists of two modules: an athermal 25mm F/2.3 mid-wave IR objective lens and an optional panoramic adapter. The adapter utilizes anamorphic lenses to create unique image control. The result of which enables an independent horizontal wide field of view, while preserving the original narrow vertical field. We have designed, fabricated and tested two such lenses. A summary of the assembly and testing process is also presented.

  8. Feasibility of 4D flow MR imaging of the brain with either Cartesian y-z radial sampling or k-t SENSE: comparison with 4D Flow MR imaging using SENSE.

    PubMed

    Sekine, Tetsuro; Amano, Yasuo; Takagi, Ryo; Matsumura, Yoshio; Murai, Yasuo; Kumita, Shinichiro

    2014-01-01

    A drawback of time-resolved 3-dimensional phase contrast magnetic resonance (4D Flow MR) imaging is its lengthy scan time for clinical application in the brain. We assessed the feasibility for flow measurement and visualization of 4D Flow MR imaging using Cartesian y-z radial sampling and that using k-t sensitivity encoding (k-t SENSE) by comparison with the standard scan using SENSE. Sixteen volunteers underwent 3 types of 4D Flow MR imaging of the brain using a 3.0-tesla scanner. As the standard scan, 4D Flow MR imaging with SENSE was performed first and then followed by 2 types of acceleration scan-with Cartesian y-z radial sampling and with k-t SENSE. We measured peak systolic velocity (PSV) and blood flow volume (BFV) in 9 arteries, and the percentage of particles arriving from the emitter plane at the target plane in 3 arteries, visually graded image quality in 9 arteries, and compared these quantitative and visual data between the standard scan and each acceleration scan. 4D Flow MR imaging examinations were completed in all but one volunteer, who did not undergo the last examination because of headache. Each acceleration scan reduced scan time by 50% compared with the standard scan. The k-t SENSE imaging underestimated PSV and BFV (P < 0.05). There were significant correlations for PSV and BFV between the standard scan and each acceleration scan (P < 0.01). The percentage of particles reaching the target plane did not differ between the standard scan and each acceleration scan. For visual assessment, y-z radial sampling deteriorated the image quality of the 3 arteries. Cartesian y-z radial sampling is feasible for measuring flow, and k-t SENSE offers sufficient flow visualization; both allow acquisition of 4D Flow MR imaging with shorter scan time.

  9. A survey on object detection in optical remote sensing images

    NASA Astrophysics Data System (ADS)

    Cheng, Gong; Han, Junwei

    2016-07-01

    Object detection in optical remote sensing images, being a fundamental but challenging problem in the field of aerial and satellite image analysis, plays an important role for a wide range of applications and is receiving significant attention in recent years. While enormous methods exist, a deep review of the literature concerning generic object detection is still lacking. This paper aims to provide a review of the recent progress in this field. Different from several previously published surveys that focus on a specific object class such as building and road, we concentrate on more generic object categories including, but are not limited to, road, building, tree, vehicle, ship, airport, urban-area. Covering about 270 publications we survey (1) template matching-based object detection methods, (2) knowledge-based object detection methods, (3) object-based image analysis (OBIA)-based object detection methods, (4) machine learning-based object detection methods, and (5) five publicly available datasets and three standard evaluation metrics. We also discuss the challenges of current studies and propose two promising research directions, namely deep learning-based feature representation and weakly supervised learning-based geospatial object detection. It is our hope that this survey will be beneficial for the researchers to have better understanding of this research field.

  10. Extraction of Extended Small-Scale Objects in Digital Images

    NASA Astrophysics Data System (ADS)

    Volkov, V. Y.

    2015-05-01

    Detection and localization problem of extended small-scale objects with different shapes appears in radio observation systems which use SAR, infra-red, lidar and television camera. Intensive non-stationary background is the main difficulty for processing. Other challenge is low quality of images, blobs, blurred boundaries; in addition SAR images suffer from a serious intrinsic speckle noise. Statistics of background is not normal, it has evident skewness and heavy tails in probability density, so it is hard to identify it. The problem of extraction small-scale objects is solved here on the basis of directional filtering, adaptive thresholding and morthological analysis. New kind of masks is used which are open-ended at one side so it is possible to extract ends of line segments with unknown length. An advanced method of dynamical adaptive threshold setting is investigated which is based on isolated fragments extraction after thresholding. Hierarchy of isolated fragments on binary image is proposed for the analysis of segmentation results. It includes small-scale objects with different shape, size and orientation. The method uses extraction of isolated fragments in binary image and counting points in these fragments. Number of points in extracted fragments is normalized to the total number of points for given threshold and is used as effectiveness of extraction for these fragments. New method for adaptive threshold setting and control maximises effectiveness of extraction. It has optimality properties for objects extraction in normal noise field and shows effective results for real SAR images.

  11. Multiple Object Retrieval in Image Databases Using Hierarchical Segmentation Tree

    ERIC Educational Resources Information Center

    Chen, Wei-Bang

    2012-01-01

    The purpose of this research is to develop a new visual information analysis, representation, and retrieval framework for automatic discovery of salient objects of user's interest in large-scale image databases. In particular, this dissertation describes a content-based image retrieval framework which supports multiple-object retrieval. The…

  12. Terahertz holography for imaging amplitude and phase objects.

    PubMed

    Hack, Erwin; Zolliker, Peter

    2014-06-30

    A non-monochromatic THz Quantum Cascade Laser and an uncooled micro-bolometer array detector with VGA resolution are used in a beam-splitter free holographic set-up to measure amplitude and phase objects in transmission. Phase maps of the diffraction pattern are retrieved using the Fourier transform carrier fringe method; while a Fresnel-Kirchhoff back propagation algorithm is used to reconstruct the complex object image. A lateral resolution of 280 µm and a relative phase sensitivity of about 0.5 rad are estimated from reconstructed images of a metallic Siemens star and a polypropylene test structure, respectively. Simulations corroborate the experimental results.

  13. Single-Shot MR Spectroscopic Imaging with Partial Parallel Imaging

    PubMed Central

    Posse, Stefan; Otazo, Ricardo; Tsai, Shang-Yueh; Yoshimoto, Akio Ernesto; Lin, Fa-Hsuan

    2010-01-01

    An MR spectroscopic imaging (MRSI) pulse sequence based on Proton-Echo-Planar-Spectroscopic-Imaging (PEPSI) is introduced that measures 2-dimensional metabolite maps in a single excitation. Echo-planar spatial-spectral encoding was combined with interleaved phase encoding and parallel imaging using SENSE to reconstruct absorption mode spectra. The symmetrical k-space trajectory compensates phase errors due to convolution of spatial and spectral encoding. Single-shot MRSI at short TE was evaluated in phantoms and in vivo on a 3 T whole body scanner equipped with 12-channel array coil. Four-step interleaved phase encoding and 4-fold SENSE acceleration were used to encode a 16×16 spatial matrix with 390 Hz spectral width. Comparison with conventional PEPSI and PEPSI with 4-fold SENSE acceleration demonstrated comparable sensitivity per unit time when taking into account g-factor related noise increases and differences in sampling efficiency. LCModel fitting enabled quantification of Inositol, Choline, Creatine and NAA in vivo with concentration values in the ranges measured with conventional PEPSI and SENSE-accelerated PEPSI. Cramer-Rao lower bounds were comparable to those obtained with conventional SENSE-accelerated PEPSI at the same voxel size and measurement time. This single-shot MRSI method is therefore suitable for applications that require high temporal resolution to monitor temporal dynamics or to reduce sensitivity to tissue movement. PMID:19097245

  14. A CONSTRAINT ON QUASAR CLUSTERING AT z = 5 FROM A BINARY QUASAR

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McGreer, Ian D.; Fan, Xiaohui; Eftekharzadeh, Sarah

    2016-03-15

    We report the discovery of a quasar pair at z = 5 separated by 21″. Both objects were identified as quasar candidates using simple color selection techniques applied to photometric catalogs from the Canada–France–Hawaii Telescope (CFHT) Legacy Survey (CFHTLS). Spectra obtained with the MMT present no discernible offset in redshift between the two objects; on the other hand, there are clear differences in the emission line profiles and in the multiwavelength spectral energy distributions that strongly disfavor the hypothesis that they are gravitationally lensed images of a single quasar. Both quasars are surprisingly bright given their proximity (a projected separation of ∼135more » kpc), with i = 19.4 and i = 21.4. Previous measurements of the luminosity function demonstrate that luminous quasars are extremely rare at z = 5; the existence of this pair suggests that quasars have strong small-scale clustering at high redshift. Assuming a real-space correlation function of the form ξ(r) ∝ (r/r{sub 0}){sup −2}, this discovery implies a correlation length of r{sub 0} ≳ 20h{sup −1} Mpc, consistent with a rapid strengthening of quasar clustering at high redshift as seen in previous observations and predicted by theoretical models where feedback effects are inefficient at shutting down black hole growth at high redshift.« less

  15. [Object Separation from Medical X-Ray Images Based on ICA].

    PubMed

    Li, Yan; Yu, Chun-yu; Miao, Ya-jian; Fei, Bin; Zhuang, Feng-yun

    2015-03-01

    X-ray medical image can examine diseased tissue of patients and has important reference value for medical diagnosis. With the problems that traditional X-ray images have noise, poor level sense and blocked aliasing organs, this paper proposes a method for the introduction of multi-spectrum X-ray imaging and independent component analysis (ICA) algorithm to separate the target object. Firstly image de-noising preprocessing ensures the accuracy of target extraction based on independent component analysis and sparse code shrinkage. Then according to the main proportion of organ in the images, aliasing thickness matrix of each pixel was isolated. Finally independent component analysis obtains convergence matrix to reconstruct the target object with blind separation theory. In the ICA algorithm, it found that when the number is more than 40, the target objects separate successfully with the aid of subjective evaluation standard. And when the amplitudes of the scale are in the [25, 45] interval, the target images have high contrast and less distortion. The three-dimensional figure of Peak signal to noise ratio (PSNR) shows that the different convergence times and amplitudes have a greater influence on image quality. The contrast and edge information of experimental images achieve better effects with the convergence times 85 and amplitudes 35 in the ICA algorithm.

  16. Technique for identifying, tracing, or tracking objects in image data

    DOEpatents

    Anderson, Robert J [Albuquerque, NM; Rothganger, Fredrick [Albuquerque, NM

    2012-08-28

    A technique for computer vision uses a polygon contour to trace an object. The technique includes rendering a polygon contour superimposed over a first frame of image data. The polygon contour is iteratively refined to more accurately trace the object within the first frame after each iteration. The refinement includes computing image energies along lengths of contour lines of the polygon contour and adjusting positions of the contour lines based at least in part on the image energies.

  17. Analysis and Recognition of Curve Type as The Basis of Object Recognition in Image

    NASA Astrophysics Data System (ADS)

    Nugraha, Nurma; Madenda, Sarifuddin; Indarti, Dina; Dewi Agushinta, R.; Ernastuti

    2016-06-01

    An object in an image when analyzed further will show the characteristics that distinguish one object with another object in an image. Characteristics that are used in object recognition in an image can be a color, shape, pattern, texture and spatial information that can be used to represent objects in the digital image. The method has recently been developed for image feature extraction on objects that share characteristics curve analysis (simple curve) and use the search feature of chain code object. This study will develop an algorithm analysis and the recognition of the type of curve as the basis for object recognition in images, with proposing addition of complex curve characteristics with maximum four branches that will be used for the process of object recognition in images. Definition of complex curve is the curve that has a point of intersection. By using some of the image of the edge detection, the algorithm was able to do the analysis and recognition of complex curve shape well.

  18. Fast processing of microscopic images using object-based extended depth of field.

    PubMed

    Intarapanich, Apichart; Kaewkamnerd, Saowaluck; Pannarut, Montri; Shaw, Philip J; Tongsima, Sissades

    2016-12-22

    Microscopic analysis requires that foreground objects of interest, e.g. cells, are in focus. In a typical microscopic specimen, the foreground objects may lie on different depths of field necessitating capture of multiple images taken at different focal planes. The extended depth of field (EDoF) technique is a computational method for merging images from different depths of field into a composite image with all foreground objects in focus. Composite images generated by EDoF can be applied in automated image processing and pattern recognition systems. However, current algorithms for EDoF are computationally intensive and impractical, especially for applications such as medical diagnosis where rapid sample turnaround is important. Since foreground objects typically constitute a minor part of an image, the EDoF technique could be made to work much faster if only foreground regions are processed to make the composite image. We propose a novel algorithm called object-based extended depths of field (OEDoF) to address this issue. The OEDoF algorithm consists of four major modules: 1) color conversion, 2) object region identification, 3) good contrast pixel identification and 4) detail merging. First, the algorithm employs color conversion to enhance contrast followed by identification of foreground pixels. A composite image is constructed using only these foreground pixels, which dramatically reduces the computational time. We used 250 images obtained from 45 specimens of confirmed malaria infections to test our proposed algorithm. The resulting composite images with all in-focus objects were produced using the proposed OEDoF algorithm. We measured the performance of OEDoF in terms of image clarity (quality) and processing time. The features of interest selected by the OEDoF algorithm are comparable in quality with equivalent regions in images processed by the state-of-the-art complex wavelet EDoF algorithm; however, OEDoF required four times less processing time. This

  19. Visual object imagery and autobiographical memory: Object Imagers are better at remembering their personal past.

    PubMed

    Vannucci, Manila; Pelagatti, Claudia; Chiorri, Carlo; Mazzoni, Giuliana

    2016-01-01

    In the present study we examined whether higher levels of object imagery, a stable characteristic that reflects the ability and preference in generating pictorial mental images of objects, facilitate involuntary and voluntary retrieval of autobiographical memories (ABMs). Individuals with high (High-OI) and low (Low-OI) levels of object imagery were asked to perform an involuntary and a voluntary ABM task in the laboratory. Results showed that High-OI participants generated more involuntary and voluntary ABMs than Low-OI, with faster retrieval times. High-OI also reported more detailed memories compared to Low-OI and retrieved memories as visual images. Theoretical implications of these findings for research on voluntary and involuntary ABMs are discussed.

  20. Objective quality assessment of tone-mapped images.

    PubMed

    Yeganeh, Hojatollah; Wang, Zhou

    2013-02-01

    Tone-mapping operators (TMOs) that convert high dynamic range (HDR) to low dynamic range (LDR) images provide practically useful tools for the visualization of HDR images on standard LDR displays. Different TMOs create different tone-mapped images, and a natural question is which one has the best quality. Without an appropriate quality measure, different TMOs cannot be compared, and further improvement is directionless. Subjective rating may be a reliable evaluation method, but it is expensive and time consuming, and more importantly, is difficult to be embedded into optimization frameworks. Here we propose an objective quality assessment algorithm for tone-mapped images by combining: 1) a multiscale signal fidelity measure on the basis of a modified structural similarity index and 2) a naturalness measure on the basis of intensity statistics of natural images. Validations using independent subject-rated image databases show good correlations between subjective ranking score and the proposed tone-mapped image quality index (TMQI). Furthermore, we demonstrate the extended applications of TMQI using two examples-parameter tuning for TMOs and adaptive fusion of multiple tone-mapped images.

  1. A review of supervised object-based land-cover image classification

    NASA Astrophysics Data System (ADS)

    Ma, Lei; Li, Manchun; Ma, Xiaoxue; Cheng, Liang; Du, Peijun; Liu, Yongxue

    2017-08-01

    Object-based image classification for land-cover mapping purposes using remote-sensing imagery has attracted significant attention in recent years. Numerous studies conducted over the past decade have investigated a broad array of sensors, feature selection, classifiers, and other factors of interest. However, these research results have not yet been synthesized to provide coherent guidance on the effect of different supervised object-based land-cover classification processes. In this study, we first construct a database with 28 fields using qualitative and quantitative information extracted from 254 experimental cases described in 173 scientific papers. Second, the results of the meta-analysis are reported, including general characteristics of the studies (e.g., the geographic range of relevant institutes, preferred journals) and the relationships between factors of interest (e.g., spatial resolution and study area or optimal segmentation scale, accuracy and number of targeted classes), especially with respect to the classification accuracy of different sensors, segmentation scale, training set size, supervised classifiers, and land-cover types. Third, useful data on supervised object-based image classification are determined from the meta-analysis. For example, we find that supervised object-based classification is currently experiencing rapid advances, while development of the fuzzy technique is limited in the object-based framework. Furthermore, spatial resolution correlates with the optimal segmentation scale and study area, and Random Forest (RF) shows the best performance in object-based classification. The area-based accuracy assessment method can obtain stable classification performance, and indicates a strong correlation between accuracy and training set size, while the accuracy of the point-based method is likely to be unstable due to mixed objects. In addition, the overall accuracy benefits from higher spatial resolution images (e.g., unmanned aerial

  2. Moving object detection in top-view aerial videos improved by image stacking

    NASA Astrophysics Data System (ADS)

    Teutsch, Michael; Krüger, Wolfgang; Beyerer, Jürgen

    2017-08-01

    Image stacking is a well-known method that is used to improve the quality of images in video data. A set of consecutive images is aligned by applying image registration and warping. In the resulting image stack, each pixel has redundant information about its intensity value. This redundant information can be used to suppress image noise, resharpen blurry images, or even enhance the spatial image resolution as done in super-resolution. Small moving objects in the videos usually get blurred or distorted by image stacking and thus need to be handled explicitly. We use image stacking in an innovative way: image registration is applied to small moving objects only, and image warping blurs the stationary background that surrounds the moving objects. Our video data are coming from a small fixed-wing unmanned aerial vehicle (UAV) that acquires top-view gray-value images of urban scenes. Moving objects are mainly cars but also other vehicles such as motorcycles. The resulting images, after applying our proposed image stacking approach, are used to improve baseline algorithms for vehicle detection and segmentation. We improve precision and recall by up to 0.011, which corresponds to a reduction of the number of false positive and false negative detections by more than 3 per second. Furthermore, we show how our proposed image stacking approach can be implemented efficiently.

  3. Improving the ability of image sensors to detect faint stars and moving objects using image deconvolution techniques.

    PubMed

    Fors, Octavi; Núñez, Jorge; Otazu, Xavier; Prades, Albert; Cardinal, Robert D

    2010-01-01

    In this paper we show how the techniques of image deconvolution can increase the ability of image sensors as, for example, CCD imagers, to detect faint stars or faint orbital objects (small satellites and space debris). In the case of faint stars, we show that this benefit is equivalent to double the quantum efficiency of the used image sensor or to increase the effective telescope aperture by more than 30% without decreasing the astrometric precision or introducing artificial bias. In the case of orbital objects, the deconvolution technique can double the signal-to-noise ratio of the image, which helps to discover and control dangerous objects as space debris or lost satellites. The benefits obtained using CCD detectors can be extrapolated to any kind of image sensors.

  4. ZaP-HD: High Energy Density Z-Pinch Plasmas using Sheared Flow Stabilization

    NASA Astrophysics Data System (ADS)

    Golingo, R. P.; Shumlak, U.; Nelson, B. A.; Claveau, E. L.; Doty, S. A.; Forbes, E. G.; Hughes, M. C.; Kim, B.; Ross, M. P.; Weed, J. R.

    2015-11-01

    The ZaP-HD flow Z-pinch project investigates scaling the flow Z-pinch to High Energy Density Plasma, HEDP, conditions by using sheared flow stabilization. ZaP used a single power supply to produce 100 cm long Z-pinches that were quiescent for many radial Alfven times and axial flow-through times. The flow Z-pinch concept provides an approach to achieve HED plasmas, which are dimensionally large and persist for extended durations. The ZaP-HD device replaces the single power supply from ZaP with two separate power supplies to independently control the plasma flow and current in the Z-pinch. Equilibrium is determined by diagnostic measurements of the density with interferometry and digital holography, the plasma flow and temperature with passive spectroscopy, the magnetic field with surface magnetic probes, and plasma emission with optical imaging. The diagnostics fully characterize the plasma from its initiation in the coaxial accelerator, through the pinch, and exhaust from the assembly region. The plasma evolution is modeled with high resolution codes: Mach2, WARPX, and NIMROD. Experimental results and scaling analyses are presented. This work is supported by grants from the U.S. Department of Energy and the U.S. National Nuclear Security Administration.

  5. Method for detecting an image of an object

    DOEpatents

    Chapman, Leroy Dean; Thomlinson, William C.; Zhong, Zhong

    1999-11-16

    A method for detecting an absorption, refraction and scatter image of an object by independently analyzing, detecting, digitizing, and combining images acquired on a high and a low angle side of a rocking curve of a crystal analyzer. An x-ray beam which is generated by any suitable conventional apparatus can be irradiated upon either a Bragg type crystal analyzer or a Laue type crystal analyzer. Images of the absorption, refraction and scattering effects are detected, such as on an image plate, and then digitized. The digitized images are simultaneously solved, preferably on a pixel-by-pixel basis, to derive a combined visual image which has dramatically improved contrast and spatial resolution over an image acquired through conventional radiology methods.

  6. RecceMan: an interactive recognition assistance for image-based reconnaissance: synergistic effects of human perception and computational methods for object recognition, identification, and infrastructure analysis

    NASA Astrophysics Data System (ADS)

    El Bekri, Nadia; Angele, Susanne; Ruckhäberle, Martin; Peinsipp-Byma, Elisabeth; Haelke, Bruno

    2015-10-01

    This paper introduces an interactive recognition assistance system for imaging reconnaissance. This system supports aerial image analysts on missions during two main tasks: Object recognition and infrastructure analysis. Object recognition concentrates on the classification of one single object. Infrastructure analysis deals with the description of the components of an infrastructure and the recognition of the infrastructure type (e.g. military airfield). Based on satellite or aerial images, aerial image analysts are able to extract single object features and thereby recognize different object types. It is one of the most challenging tasks in the imaging reconnaissance. Currently, there are no high potential ATR (automatic target recognition) applications available, as consequence the human observer cannot be replaced entirely. State-of-the-art ATR applications cannot assume in equal measure human perception and interpretation. Why is this still such a critical issue? First, cluttered and noisy images make it difficult to automatically extract, classify and identify object types. Second, due to the changed warfare and the rise of asymmetric threats it is nearly impossible to create an underlying data set containing all features, objects or infrastructure types. Many other reasons like environmental parameters or aspect angles compound the application of ATR supplementary. Due to the lack of suitable ATR procedures, the human factor is still important and so far irreplaceable. In order to use the potential benefits of the human perception and computational methods in a synergistic way, both are unified in an interactive assistance system. RecceMan® (Reconnaissance Manual) offers two different modes for aerial image analysts on missions: the object recognition mode and the infrastructure analysis mode. The aim of the object recognition mode is to recognize a certain object type based on the object features that originated from the image signatures. The

  7. SHERPA: an image segmentation and outline feature extraction tool for diatoms and other objects

    PubMed Central

    2014-01-01

    Background Light microscopic analysis of diatom frustules is widely used both in basic and applied research, notably taxonomy, morphometrics, water quality monitoring and paleo-environmental studies. In these applications, usually large numbers of frustules need to be identified and/or measured. Although there is a need for automation in these applications, and image processing and analysis methods supporting these tasks have previously been developed, they did not become widespread in diatom analysis. While methodological reports for a wide variety of methods for image segmentation, diatom identification and feature extraction are available, no single implementation combining a subset of these into a readily applicable workflow accessible to diatomists exists. Results The newly developed tool SHERPA offers a versatile image processing workflow focused on the identification and measurement of object outlines, handling all steps from image segmentation over object identification to feature extraction, and providing interactive functions for reviewing and revising results. Special attention was given to ease of use, applicability to a broad range of data and problems, and supporting high throughput analyses with minimal manual intervention. Conclusions Tested with several diatom datasets from different sources and of various compositions, SHERPA proved its ability to successfully analyze large amounts of diatom micrographs depicting a broad range of species. SHERPA is unique in combining the following features: application of multiple segmentation methods and selection of the one giving the best result for each individual object; identification of shapes of interest based on outline matching against a template library; quality scoring and ranking of resulting outlines supporting quick quality checking; extraction of a wide range of outline shape descriptors widely used in diatom studies and elsewhere; minimizing the need for, but enabling manual quality control and

  8. Three dimensional live-cell STED microscopy at increased depth using a water immersion objective

    NASA Astrophysics Data System (ADS)

    Heine, Jörn; Wurm, Christian A.; Keller-Findeisen, Jan; Schönle, Andreas; Harke, Benjamin; Reuss, Matthias; Winter, Franziska R.; Donnert, Gerald

    2018-05-01

    Modern fluorescence superresolution microscopes are capable of imaging living cells on the nanometer scale. One of those techniques is stimulated emission depletion (STED) which increases the microscope's resolution many times in the lateral and the axial directions. To achieve these high resolutions not only close to the coverslip but also at greater depths, the choice of objective becomes crucial. Oil immersion objectives have frequently been used for STED imaging since their high numerical aperture (NA) leads to high spatial resolutions. But during live-cell imaging, especially at great penetration depths, these objectives have a distinct disadvantage. The refractive index mismatch between the immersion oil and the usually aqueous embedding media of living specimens results in unwanted spherical aberrations. These aberrations distort the point spread functions (PSFs). Notably, during z- and 3D-STED imaging, the resolution increase along the optical axis is majorly hampered if at all possible. To overcome this limitation, we here use a water immersion objective in combination with a spatial light modulator for z-STED measurements of living samples at great depths. This compact design allows for switching between objectives without having to adapt the STED beam path and enables on the fly alterations of the STED PSF to correct for aberrations. Furthermore, we derive the influence of the NA on the axial STED resolution theoretically and experimentally. We show under live-cell imaging conditions that a water immersion objective leads to far superior results than an oil immersion objective at penetration depths of 5-180 μm.

  9. Coronal plasma development in wire-array z-pinches made of twisted-pairs

    NASA Astrophysics Data System (ADS)

    Hoyt, C. L.; Greenly, J. B.; Gourdain, P. A.; Knapp, P. F.; Pikuz, S. A.; Shelkovenko, T. A.; Hammer, D. A.; Kusse, B. R.

    2009-11-01

    We have investigated coronal and core plasma development in wire array z-pinches in which single fine wires are replaced by twisted-pairs (``cable'') on the 1 MA, 100 ns rise time COBRA pulsed power generator. X-ray radiography, employed to investigate dense wire core expansion, showed periodic axial nonuniformity and evidence for shock waves developing where the individual wire plasmas collide. Laser shadowgraphy images indicated that the axial instability properties of the coronal plasma are substantially modified from ordinary wire arrays. Cable mass per unit length, material and the twist wavelength were varied in order to study their effects upon the instability wavelength. Implosion uniformity and bright-spot formation, as well as magnetic topology evolution, have also been investigated using self-emission imaging, x-ray diagnostics and small B-dot probes, respectively. Results from the cable-array z-pinches will be compared with results from ordinary wire-array z-pinches. This research was supported by the SSAA program of the National Nuclear Security Administration under DOE Cooperative agreement DE-FC03-02NA00057.

  10. The measurement of ultrasound scattering from individual micron-sized objects and its application in single cell scattering.

    PubMed

    Falou, Omar; Rui, Min; El Kaffas, Ahmed; Kumaradas, J Carl; Kolios, Michael C

    2010-08-01

    The measurement of the ultrasound backscatter from individual micron-sized objects such as cells is required for various applications such as tissue characterization. However, performing such a measurement remains a challenge. For example, the presence of air bubbles in a suspension of cells during the measurements may lead to the incorrect interpretation of the acoustic signals. This work introduces a technique for measuring the ultrasound backscatter from individual micron-sized objects by combining a microinjection system with a co-registered optical microscope and an ultrasound imaging device. This allowed the measurement of the ultrasound backscatter response from a single object under optical microscope guidance. The optical and ultrasonic data were used to determine the size of the object and to deduce its backscatter responses, respectively. In order to calibrate the system, the backscatter frequency responses from polystyrene microspheres were measured and compared to theoretical predictions. A very good agreement was found between the measured backscatter responses of individual microspheres and theoretical predictions of an elastic sphere. The backscatter responses from single OCI-AML-5 cells were also investigated. It was found that the backscatter responses from AML cells are best modeled using the fluid sphere model. The advantages, limitations, and future applications of the developed technique are discussed.

  11. Optical imaging of objects in turbid medium with ultrashort pulses

    NASA Astrophysics Data System (ADS)

    Wang, Chih-Yu; Sun, Chia-Wei; Yang, Chih Chung; Kiang, Yean-Woei; Lin, Chii-Wann

    2000-07-01

    Photons are seriously scattered when entering turbid medium; this the images of objects hidden in turbid medium can not be obtained by just collecting the transmitted photons. Early-arriving photons, which are also called ballistic or snake protons, are much less scattered when passing through turbid medium, and contains more image information than the late-arriving ones. Therefore, objects embedded in turbid medium can be imaged by gathering the ballistic and snake photons. In the present research we try to recover images of objects in turbid medium by simultaneously time-gate and polarization-gate to obtain the snake photons. An Argon-pumped Ti-Sapphire laser with 100fs pulses was employed as a light source. A streak camera with a 2ps temporal resolution was used to extract the ballistic and snake photons. Two pieces of lean swine meat, measured 4mmX3mm and 5xxX4mm, respectively, were placed in a 10cmX10cmX3cm acrylic tank, which was full of diluted milk. A pair of polarizer and an analyzer was used to extract the light that keeps polarization unchanged. The combination of time gating and polarization gating resulted in good images of objects hidden in turbid medium.

  12. Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking

    PubMed Central

    von Diezmann, Alex; Shechtman, Yoav; Moerner, W. E.

    2017-01-01

    Single-molecule super-resolution fluorescence microscopy and single-particle tracking are two imaging modalities that illuminate the properties of cells and materials on spatial scales down to tens of nanometers, or with dynamical information about nanoscale particle motion in the millisecond range, respectively. These methods generally use wide-field microscopes and two-dimensional camera detectors to localize molecules to much higher precision than the diffraction limit. Given the limited total photons available from each single-molecule label, both modalities require careful mathematical analysis and image processing. Much more information can be obtained about the system under study by extending to three-dimensional (3D) single-molecule localization: without this capability, visualization of structures or motions extending in the axial direction can easily be missed or confused, compromising scientific understanding. A variety of methods for obtaining both 3D super-resolution images and 3D tracking information have been devised, each with their own strengths and weaknesses. These include imaging of multiple focal planes, point-spread-function engineering, and interferometric detection. These methods may be compared based on their ability to provide accurate and precise position information of single-molecule emitters with limited photons. To successfully apply and further develop these methods, it is essential to consider many practical concerns, including the effects of optical aberrations, field-dependence in the imaging system, fluorophore labeling density, and registration between different color channels. Selected examples of 3D super-resolution imaging and tracking are described for illustration from a variety of biological contexts and with a variety of methods, demonstrating the power of 3D localization for understanding complex systems. PMID:28151646

  13. Photoacoustic imaging of single circulating melanoma cells in vivo

    NASA Astrophysics Data System (ADS)

    Wang, Lidai; Yao, Junjie; Zhang, Ruiying; Xu, Song; Li, Guo; Zou, Jun; Wang, Lihong V.

    2015-03-01

    Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.

  14. Objective for EUV microscopy, EUV lithography, and x-ray imaging

    DOEpatents

    Bitter, Manfred; Hill, Kenneth W.; Efthimion, Philip

    2016-05-03

    Disclosed is an imaging apparatus for EUV spectroscopy, EUV microscopy, EUV lithography, and x-ray imaging. This new imaging apparatus could, in particular, make significant contributions to EUV lithography at wavelengths in the range from 10 to 15 nm, which is presently being developed for the manufacturing of the next-generation integrated circuits. The disclosure provides a novel adjustable imaging apparatus that allows for the production of stigmatic images in x-ray imaging, EUV imaging, and EUVL. The imaging apparatus of the present invention incorporates additional properties compared to previously described objectives. The use of a pair of spherical reflectors containing a concave and convex arrangement has been applied to a EUV imaging system to allow for the image and optics to all be placed on the same side of a vacuum chamber. Additionally, the two spherical reflector segments previously described have been replaced by two full spheres or, more precisely, two spherical annuli, so that the total photon throughput is largely increased. Finally, the range of permissible Bragg angles and possible magnifications of the objective has been largely increased.

  15. Improving the Ability of Image Sensors to Detect Faint Stars and Moving Objects Using Image Deconvolution Techniques

    PubMed Central

    Fors, Octavi; Núñez, Jorge; Otazu, Xavier; Prades, Albert; Cardinal, Robert D.

    2010-01-01

    In this paper we show how the techniques of image deconvolution can increase the ability of image sensors as, for example, CCD imagers, to detect faint stars or faint orbital objects (small satellites and space debris). In the case of faint stars, we show that this benefit is equivalent to double the quantum efficiency of the used image sensor or to increase the effective telescope aperture by more than 30% without decreasing the astrometric precision or introducing artificial bias. In the case of orbital objects, the deconvolution technique can double the signal-to-noise ratio of the image, which helps to discover and control dangerous objects as space debris or lost satellites. The benefits obtained using CCD detectors can be extrapolated to any kind of image sensors. PMID:22294896

  16. Computational imaging with a single-pixel detector and a consumer video projector

    NASA Astrophysics Data System (ADS)

    Sych, D.; Aksenov, M.

    2018-02-01

    Single-pixel imaging is a novel rapidly developing imaging technique that employs spatially structured illumination and a single-pixel detector. In this work, we experimentally demonstrate a fully operating modular single-pixel imaging system. Light patterns in our setup are created with help of a computer-controlled digital micromirror device from a consumer video projector. We investigate how different working modes and settings of the projector affect the quality of reconstructed images. We develop several image reconstruction algorithms and compare their performance for real imaging. Also, we discuss the potential use of the single-pixel imaging system for quantum applications.

  17. Finding Kuiper Belt Objects Below the Detection Limit

    NASA Astrophysics Data System (ADS)

    Whidden, Peter; Kalmbach, Bryce; Bektesevic, Dino; Connolly, Andrew; Jones, Lynne; Smotherman, Hayden; Becker, Andrew

    2018-01-01

    We demonstrate a novel approach for uncovering the signatures of moving objects (e.g. Kuiper Belt Objects) below the detection thresholds of single astronomical images. To do so, we will employ a matched filter moving at specific rates of proposed orbits through a time-domain dataset. This is analogous to the better-known "shift-and-stack" method; however it uses neither direct shifting nor stacking of the image pixels. Instead of resampling the raw pixels to create an image stack, we will instead integrate the object detection probabilities across multiple single-epoch images to accrue support for a proposed orbit. The filtering kernel provides a measure of the probability that an object is present along a given orbit, and enables the user to make principled decisions about when the search has been successful, and when it may be terminated. The results we present here utilize GPUs to speed up the search by two orders of magnitudes over CPU implementations.

  18. Stochastic resonance investigation of object detection in images

    NASA Astrophysics Data System (ADS)

    Repperger, Daniel W.; Pinkus, Alan R.; Skipper, Julie A.; Schrider, Christina D.

    2007-02-01

    Object detection in images was conducted using a nonlinear means of improving signal to noise ratio termed "stochastic resonance" (SR). In a recent United States patent application, it was shown that arbitrarily large signal to noise ratio gains could be realized when a signal detection problem is cast within the context of a SR filter. Signal-to-noise ratio measures were investigated. For a binary object recognition task (friendly versus hostile), the method was implemented by perturbing the recognition algorithm and subsequently thresholding via a computer simulation. To fairly test the efficacy of the proposed algorithm, a unique database of images has been constructed by modifying two sample library objects by adjusting their brightness, contrast and relative size via commercial software to gradually compromise their saliency to identification. The key to the use of the SR method is to produce a small perturbation in the identification algorithm and then to threshold the results, thus improving the overall system's ability to discern objects. A background discussion of the SR method is presented. A standard test is proposed in which object identification algorithms could be fairly compared against each other with respect to their relative performance.

  19. Mental visualization of objects from cross-sectional images

    PubMed Central

    Wu, Bing; Klatzky, Roberta L.; Stetten, George D.

    2011-01-01

    We extended the classic anorthoscopic viewing procedure to test a model of visualization of 3D structures from 2D cross-sections. Four experiments were conducted to examine key processes described in the model, localizing cross-sections within a common frame of reference and spatiotemporal integration of cross sections into a hierarchical object representation. Participants used a hand-held device to reveal a hidden object as a sequence of cross-sectional images. The process of localization was manipulated by contrasting two displays, in-situ vs. ex-situ, which differed in whether cross sections were presented at their source locations or displaced to a remote screen. The process of integration was manipulated by varying the structural complexity of target objects and their components. Experiments 1 and 2 demonstrated visualization of 2D and 3D line-segment objects and verified predictions about display and complexity effects. In Experiments 3 and 4, the visualized forms were familiar letters and numbers. Errors and orientation effects showed that displacing cross-sectional images to a remote display (ex-situ viewing) impeded the ability to determine spatial relationships among pattern components, a failure of integration at the object level. PMID:22217386

  20. Subaru High-z Exploration of Low-Luminosity Quasars (SHELLQs): New z > 6 Quasar Survey with Subaru/HSC

    NASA Astrophysics Data System (ADS)

    Matsuoka, Yoshiki; SHELLQs Collaboration

    2017-01-01

    Quasars at high redshift are an important and unique probe of the distant Universe, for understanding the origin and progress of cosmic reionization, the early growth of supermassive black holes, and the evolution of quasar host galaxies and their dark matter halos, among other topics. We are currently carrying out a new spectroscopic survey, called SHELLQs (Subaru High-z Exploration of Low-Luminosity Quasars), to search for low-luminosity quasars at z > 6. By exploiting the exquisite imaging data produced by the Subaru Hyper Suprime-Cam (HSC) survey, we aim to probe quasar luminosities down to M1450 ~ -22 mag, i.e., below the classical threshold between quasars and Seyfert galaxies. Candidate selection is performed by combining several photometric approaches including a Bayesian probabilistic algorithm. A large spectroscopic observing program is underway, using Subaru/FOCAS, GTC/OSIRIS, and Gemini/GMOS; in particular, SHELLQs has been approved as a Subaru intensive program to use 20 nights in the coming four semesters. As of August 2016, we have discovered ~40 quasars and bright galaxies at z ~ 6 and beyond, from the first 100 deg2 of the HSC survey (Matsuoka et al. 2016, ApJ, 828, 26). Surprisingly, we are starting to see the steep rise of the luminosity function of high-z galaxies, compared with that of quasars, at magnitudes fainter than M1450 ~ -22 mag or zAB ~ 24 mag. Multi-wavelength follow-up studies of the discovered objects as well as further survey observations are ongoing.

  1. Tracker: Image-Processing and Object-Tracking System Developed

    NASA Technical Reports Server (NTRS)

    Klimek, Robert B.; Wright, Theodore W.

    1999-01-01

    Tracker is an object-tracking and image-processing program designed and developed at the NASA Lewis Research Center to help with the analysis of images generated by microgravity combustion and fluid physics experiments. Experiments are often recorded on film or videotape for analysis later. Tracker automates the process of examining each frame of the recorded experiment, performing image-processing operations to bring out the desired detail, and recording the positions of the objects of interest. It can load sequences of images from disk files or acquire images (via a frame grabber) from film transports, videotape, laser disks, or a live camera. Tracker controls the image source to automatically advance to the next frame. It can employ a large array of image-processing operations to enhance the detail of the acquired images and can analyze an arbitrarily large number of objects simultaneously. Several different tracking algorithms are available, including conventional threshold and correlation-based techniques, and more esoteric procedures such as "snake" tracking and automated recognition of character data in the image. The Tracker software was written to be operated by researchers, thus every attempt was made to make the software as user friendly and self-explanatory as possible. Tracker is used by most of the microgravity combustion and fluid physics experiments performed by Lewis, and by visiting researchers. This includes experiments performed on the space shuttles, Mir, sounding rockets, zero-g research airplanes, drop towers, and ground-based laboratories. This software automates the analysis of the flame or liquid s physical parameters such as position, velocity, acceleration, size, shape, intensity characteristics, color, and centroid, as well as a number of other measurements. It can perform these operations on multiple objects simultaneously. Another key feature of Tracker is that it performs optical character recognition (OCR). This feature is useful in

  2. A novel three-dimensional image reconstruction method for near-field coded aperture single photon emission computerized tomography

    PubMed Central

    Mu, Zhiping; Hong, Baoming; Li, Shimin; Liu, Yi-Hwa

    2009-01-01

    Coded aperture imaging for two-dimensional (2D) planar objects has been investigated extensively in the past, whereas little success has been achieved in imaging 3D objects using this technique. In this article, the authors present a novel method of 3D single photon emission computerized tomography (SPECT) reconstruction for near-field coded aperture imaging. Multiangular coded aperture projections are acquired and a stack of 2D images is reconstructed separately from each of the projections. Secondary projections are subsequently generated from the reconstructed image stacks based on the geometry of parallel-hole collimation and the variable magnification of near-field coded aperture imaging. Sinograms of cross-sectional slices of 3D objects are assembled from the secondary projections, and the ordered subset expectation and maximization algorithm is employed to reconstruct the cross-sectional image slices from the sinograms. Experiments were conducted using a customized capillary tube phantom and a micro hot rod phantom. Imaged at approximately 50 cm from the detector, hot rods in the phantom with diameters as small as 2.4 mm could be discerned in the reconstructed SPECT images. These results have demonstrated the feasibility of the authors’ 3D coded aperture image reconstruction algorithm for SPECT, representing an important step in their effort to develop a high sensitivity and high resolution SPECT imaging system. PMID:19544769

  3. Experimental single-chip color HDTV image acquisition system with 8M-pixel CMOS image sensor

    NASA Astrophysics Data System (ADS)

    Shimamoto, Hiroshi; Yamashita, Takayuki; Funatsu, Ryohei; Mitani, Kohji; Nojiri, Yuji

    2006-02-01

    We have developed an experimental single-chip color HDTV image acquisition system using 8M-pixel CMOS image sensor. The sensor has 3840 × 2160 effective pixels and is progressively scanned at 60 frames per second. We describe the color filter array and interpolation method to improve image quality with a high-pixel-count single-chip sensor. We also describe an experimental image acquisition system we used to measured spatial frequency characteristics in the horizontal direction. The results indicate good prospects for achieving a high quality single chip HDTV camera that reduces pseudo signals and maintains high spatial frequency characteristics within the frequency band for HDTV.

  4. Fusing Image Data for Calculating Position of an Object

    NASA Technical Reports Server (NTRS)

    Huntsberger, Terrance; Cheng, Yang; Liebersbach, Robert; Trebi-Ollenu, Ashitey

    2007-01-01

    A computer program has been written for use in maintaining the calibration, with respect to the positions of imaged objects, of a stereoscopic pair of cameras on each of the Mars Explorer Rovers Spirit and Opportunity. The program identifies and locates a known object in the images. The object in question is part of a Moessbauer spectrometer located at the tip of a robot arm, the kinematics of which are known. In the program, the images are processed through a module that extracts edges, combines the edges into line segments, and then derives ellipse centroids from the line segments. The images are also processed by a feature-extraction algorithm that performs a wavelet analysis, then performs a pattern-recognition operation in the wavelet-coefficient space to determine matches to a texture feature measure derived from the horizontal, vertical, and diagonal coefficients. The centroids from the ellipse finder and the wavelet feature matcher are then fused to determine co-location. In the event that a match is found, the centroid (or centroids if multiple matches are present) is reported. If no match is found, the process reports the results of the analyses for further examination by human experts.

  5. A cooling flow cluster at redshift Z = 0.2

    NASA Astrophysics Data System (ADS)

    Wolter, Anna; Schild, R.; Gioia, I. M.; Maccacaro, T.; Morris, S. L.; Nesci, R.; Perola, G. C.

    The cluster of galaxies 1E0839.9 + 2938, discovered in X-ray observations by Nesci et al. (1988), is characterized on the basis of VLA 6-cm radio observations, Whipple Observatory CCD photometry, and spectroscopic observations obtained with the Multiple Mirror Telescope and the 88-inch University of Hawaii Telescope at Mauna Kea. The data are presented in tables, maps, and sample images and spectra and briefly characterized. The bright X-ray object is identified with a cluster at redshift z = 0.195; its central galaxy has radio emission of 1.1 x 10 exp 24 W/Hz as well as strong optical line emission which is not restricted to its nucleus. It is concluded that 1E0839.9 + 2938 is a cooling-flow cluster similar to 3C295 (found at z = 0.461 by Henry et al., 1986). The need for space observations (by Rosat or the AXAF) to determine the object's X-ray luminosity distribution is indicated.

  6. A cooling flow cluster at redshift z = 0.2

    NASA Technical Reports Server (NTRS)

    Wolter, Anna; Schild, R.; Gioia, I. M.; Maccacaro, T.; Morris, S. L.; Nesci, R.; Perola, G. C.

    1990-01-01

    The cluster of galaxies 1E0839.9 + 2938, discovered in X-ray observations by Nesci et al. (1988), is characterized on the basis of VLA 6-cm radio observations, Whipple Observatory CCD photometry, and spectroscopic observations obtained with the Multiple Mirror Telescope and the 88-inch University of Hawaii Telescope at Mauna Kea. The data are presented in tables, maps, and sample images and spectra and briefly characterized. The bright X-ray object is identified with a cluster at redshift z = 0.195; its central galaxy has radio emission of 1.1 x 10 exp 24 W/Hz as well as strong optical line emission which is not restricted to its nucleus. It is concluded that 1E0839.9 + 2938 is a cooling-flow cluster similar to 3C295 (found at z = 0.461 by Henry et al., 1986). The need for space observations (by Rosat or the AXAF) to determine the object's X-ray luminosity distribution is indicated.

  7. Concave omnidirectional imaging device for cylindrical object based on catadioptric panoramic imaging

    NASA Astrophysics Data System (ADS)

    Wu, Xiaojun; Wu, Yumei; Wen, Peizhi

    2018-03-01

    To obtain information on the outer surface of a cylinder object, we propose a catadioptric panoramic imaging system based on the principle of uniform spatial resolution for vertical scenes. First, the influence of the projection-equation coefficients on the spatial resolution and astigmatism of the panoramic system are discussed, respectively. Through parameter optimization, we obtain the appropriate coefficients for the projection equation, and so the imaging quality of the entire imaging system can reach an optimum value. Finally, the system projection equation is calibrated, and an undistorted rectangular panoramic image is obtained using the cylindrical-surface projection expansion method. The proposed 360-deg panoramic-imaging device overcomes the shortcomings of existing surface panoramic-imaging methods, and it has the advantages of low cost, simple structure, high imaging quality, and small distortion, etc. The experimental results show the effectiveness of the proposed method.

  8. Ray tracing analysis of overlapping objects in refraction contrast imaging.

    PubMed

    Hirano, Masatsugu; Yamasaki, Katsuhito; Okada, Hiroshi; Sakurai, Takashi; Kondoh, Takeshi; Katafuchi, Tetsuro; Sugimura, Kazuro; Kitazawa, Sohei; Kitazawa, Riko; Maeda, Sakan; Tamura, Shinichi

    2005-08-01

    We simulated refraction contrast imaging in overlapping objects using the ray tracing method. The easiest case, in which two columnar objects (blood vessels) with a density of 1.0 [g/cm3], run at right angles in air, was calculated. For absorption, we performed simulation using the Snell law adapted to the object's boundary. A pair of bright and dark spot results from the interference of refracted X-rays where the blood vessels crossed. This has the possibility of increasing the visibility of the image.

  9. System and method for automated object detection in an image

    DOEpatents

    Kenyon, Garrett T.; Brumby, Steven P.; George, John S.; Paiton, Dylan M.; Schultz, Peter F.

    2015-10-06

    A contour/shape detection model may use relatively simple and efficient kernels to detect target edges in an object within an image or video. A co-occurrence probability may be calculated for two or more edge features in an image or video using an object definition. Edge features may be differentiated between in response to measured contextual support, and prominent edge features may be extracted based on the measured contextual support. The object may then be identified based on the extracted prominent edge features.

  10. 128 SHADES OF RED: Objective Remote Assessment of Radiation Dermatitis by Augmented Digital Skin Imaging.

    PubMed

    Partl, Richard; Jonko, Beata; Schnidar, Stefan; Schöllhammer, Michael; Bauer, Max; Singh, Sanchit; Simeckova, Julia; Wiesner, Kathrin; Neubauer, Andreas; Schnidar, Harald

    2017-01-01

    The purpose of our investigation was to develop a novel and state of the art digital skin imaging method capable for remote monitoring and objective assessment of Radiation Induced Dermatitis (RID). Therefore, radiation therapy related side effects were assessed by medical experts according to Common Terminology Criteria for Adverse Events (CTCAE) grade of severity in 20 female breast cancer patients in a clinical trial over the treatment time frame of 25-28 radiation cycles, 50.0-50.4 Gy each. Furthermore the intensity of developed skin erythema was documented by using conventional spectrophotometry plus digital skin imaging. Thereby we could derive the Standardized Erythema Value (SEV), a novel objective parameter, which in contrast to single parametric L* and a* delivers a long dynamic measurement range for analyzing RID from bright to very dark skin tones. Methodical superiority of the SEV could be proven over spectrophotometer measurements in terms of a higher sensitivity and by enabling signal intensity mapping in analyzed skin images. Our thereupon-derived patent enables novel objective dermatologic eHealth applications in a broad range of medical and industrial use by opening likewise the window for augmented dermatology. The first of its kind system is now already further developed in form of the medical device product Scarletred®Vision. It is available on the market for primary usage in clinical trials and in medical routine.

  11. Live-Cell Imaging of Early Steps of Single HIV-1 Infection.

    PubMed

    Francis, Ashwanth C; Melikyan, Gregory B

    2018-05-19

    Live-cell imaging of single HIV-1 entry offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 entry leading to nuclear import, integration into the host genome, and viral protein expression. Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection.

  12. Comparative analysis of imaging configurations and objectives for Fourier microscopy.

    PubMed

    Kurvits, Jonathan A; Jiang, Mingming; Zia, Rashid

    2015-11-01

    Fourier microscopy is becoming an increasingly important tool for the analysis of optical nanostructures and quantum emitters. However, achieving quantitative Fourier space measurements requires a thorough understanding of the impact of aberrations introduced by optical microscopes that have been optimized for conventional real-space imaging. Here we present a detailed framework for analyzing the performance of microscope objectives for several common Fourier imaging configurations. To this end, we model objectives from Nikon, Olympus, and Zeiss using parameters that were inferred from patent literature and confirmed, where possible, by physical disassembly. We then examine the aberrations most relevant to Fourier microscopy, including the alignment tolerances of apodization factors for different objective classes, the effect of magnification on the modulation transfer function, and vignetting-induced reductions of the effective numerical aperture for wide-field measurements. Based on this analysis, we identify an optimal objective class and imaging configuration for Fourier microscopy. In addition, the Zemax files for the objectives and setups used in this analysis have been made publicly available as a resource for future studies.

  13. Color object detection using spatial-color joint probability functions.

    PubMed

    Luo, Jiebo; Crandall, David

    2006-06-01

    Object detection in unconstrained images is an important image understanding problem with many potential applications. There has been little success in creating a single algorithm that can detect arbitrary objects in unconstrained images; instead, algorithms typically must be customized for each specific object. Consequently, it typically requires a large number of exemplars (for rigid objects) or a large amount of human intuition (for nonrigid objects) to develop a robust algorithm. We present a robust algorithm designed to detect a class of compound color objects given a single model image. A compound color object is defined as having a set of multiple, particular colors arranged spatially in a particular way, including flags, logos, cartoon characters, people in uniforms, etc. Our approach is based on a particular type of spatial-color joint probability function called the color edge co-occurrence histogram. In addition, our algorithm employs perceptual color naming to handle color variation, and prescreening to limit the search scope (i.e., size and location) for the object. Experimental results demonstrated that the proposed algorithm is insensitive to object rotation, scaling, partial occlusion, and folding, outperforming a closely related algorithm based on color co-occurrence histograms by a decisive margin.

  14. Multi-Objective vs. Single Objective Calibration of a Hydrologic Model using Either Different Hydrologic Signatures or Complementary Data Sources

    NASA Astrophysics Data System (ADS)

    Mai, J.; Cuntz, M.; Zink, M.; Schaefer, D.; Thober, S.; Samaniego, L. E.; Shafii, M.; Tolson, B.

    2015-12-01

    Hydrologic models are traditionally calibrated against discharge. Recent studies have shown however, that only a few global model parameters are constrained using the integral discharge measurements. It is therefore advisable to use additional information to calibrate those models. Snow pack data, for example, could improve the parametrization of snow-related processes, which might be underrepresented when using only discharge. One common approach is to combine these multiple objectives into one single objective function and allow the use of a single-objective algorithm. Another strategy is to consider the different objectives separately and apply a Pareto-optimizing algorithm. Both methods are challenging in the choice of appropriate multiple objectives with either conflicting interests or the focus on different model processes. A first aim of this study is to compare the two approaches employing the mesoscale Hydrologic Model mHM at several distinct river basins over Europe and North America. This comparison will allow the identification of the single-objective solution on the Pareto front. It is elucidated if this position is determined by the weighting and scaling of the multiple objectives when combing them to the single objective. The principal second aim is to guide the selection of proper objectives employing sensitivity analyses. These analyses are used to determine if an additional information would help to constrain additional model parameters. The additional information are either multiple data sources or multiple signatures of one measurement. It is evaluated if specific discharge signatures can inform different parts of the hydrologic model. The results show that an appropriate selection of discharge signatures increased the number of constrained parameters by more than 50% compared to using only NSE of the discharge time series. It is further assessed if the use of these signatures impose conflicting objectives on the hydrologic model. The usage of

  15. Atomic scale structure and chemistry of interfaces by Z-contrast imaging and electron energy loss spectroscopy in the STEM

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    McGibbon, M.M.; Browning, N.D.; Chisholm, M.F.

    The macroscopic properties of many materials are controlled by the structure and chemistry at the grain boundaries. A basic understanding of the structure-property relationship requires a technique which probes both composition and chemical bonding on an atomic scale. The high-resolution Z-contrast imaging technique in the scanning transmission electron microscope (STEM) forms an incoherent image in which changes in atomic structure and composition can be interpreted intuitively. This direct image allows the electron probe to be positioned over individual atomic columns for parallel detection electron energy loss spectroscopy (PEELS) at a spatial resolution approaching 0.22nm. The bonding information which can bemore » obtained from the fine structure within the PEELS edges can then be used in conjunction with the Z-contrast images to determine the structure at the grain boundary. In this paper we present 3 examples of correlations between the structural, chemical and electronic properties at materials interfaces in metal-semiconductor systems, superconducting and ferroelectric materials.« less

  16. Ghost imaging of phase objects with classical incoherent light

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shirai, Tomohiro; Setaelae, Tero; Friberg, Ari T.

    2011-10-15

    We describe an optical setup for performing spatial Fourier filtering in ghost imaging with classical incoherent light. This is achieved by a modification of the conventional geometry for lensless ghost imaging. It is shown on the basis of classical coherence theory that with this technique one can realize what we call phase-contrast ghost imaging to visualize pure phase objects.

  17. Objective Lens Optimized for Wavefront Delivery, Pupil Imaging, and Pupil Ghosting

    NASA Technical Reports Server (NTRS)

    Olzcak, Gene

    2009-01-01

    An interferometer objective lens (or diverger) may be used to transform a collimated beam into a diverging or converging beam. This innovation provides an objective lens that has diffraction-limited optical performance that is optimized at two sets of conjugates: imaging to the objective focus and imaging to the pupil. The lens thus provides for simultaneous delivery of a high-quality beam and excellent pupil resolution properties.

  18. Intra-individual diagnostic image quality and organ-specific-radiation dose comparison between spiral cCT with iterative image reconstruction and z-axis automated tube current modulation and sequential cCT.

    PubMed

    Wenz, Holger; Maros, Máté E; Meyer, Mathias; Gawlitza, Joshua; Förster, Alex; Haubenreisser, Holger; Kurth, Stefan; Schoenberg, Stefan O; Groden, Christoph; Henzler, Thomas

    2016-01-01

    To prospectively evaluate image quality and organ-specific-radiation dose of spiral cranial CT (cCT) combined with automated tube current modulation (ATCM) and iterative image reconstruction (IR) in comparison to sequential tilted cCT reconstructed with filtered back projection (FBP) without ATCM. 31 patients with a previous performed tilted non-contrast enhanced sequential cCT aquisition on a 4-slice CT system with only FBP reconstruction and no ATCM were prospectively enrolled in this study for a clinical indicated cCT scan. All spiral cCT examinations were performed on a 3rd generation dual-source CT system using ATCM in z-axis direction. Images were reconstructed using both, FBP and IR (level 1-5). A Monte-Carlo-simulation-based analysis was used to compare organ-specific-radiation dose. Subjective image quality for various anatomic structures was evaluated using a 4-point Likert-scale and objective image quality was evaluated by comparing signal-to-noise ratios (SNR). Spiral cCT led to a significantly lower (p < 0.05) organ-specific-radiation dose in all targets including eye lense. Subjective image quality of spiral cCT datasets with an IR reconstruction level 5 was rated significantly higher compared to the sequential cCT acquisitions (p < 0.0001). Consecutive mean SNR was significantly higher in all spiral datasets (FBP, IR 1-5) when compared to sequential cCT with a mean SNR improvement of 44.77% (p < 0.0001). Spiral cCT combined with ATCM and IR allows for significant-radiation dose reduction including a reduce eye lens organ-dose when compared to a tilted sequential cCT while improving subjective and objective image quality.

  19. Method and apparatus for acoustic imaging of objects in water

    DOEpatents

    Deason, Vance A.; Telschow, Kenneth L.

    2005-01-25

    A method, system and underwater camera for acoustic imaging of objects in water or other liquids includes an acoustic source for generating an acoustic wavefront for reflecting from a target object as a reflected wavefront. The reflected acoustic wavefront deforms a screen on an acoustic side and correspondingly deforms the opposing optical side of the screen. An optical processing system is optically coupled to the optical side of the screen and converts the deformations on the optical side of the screen into an optical intensity image of the target object.

  20. Object-Oriented Image Clustering Method Using UAS Photogrammetric Imagery

    NASA Astrophysics Data System (ADS)

    Lin, Y.; Larson, A.; Schultz-Fellenz, E. S.; Sussman, A. J.; Swanson, E.; Coppersmith, R.

    2016-12-01

    Unmanned Aerial Systems (UAS) have been used widely as an imaging modality to obtain remotely sensed multi-band surface imagery, and are growing in popularity due to their efficiency, ease of use, and affordability. Los Alamos National Laboratory (LANL) has employed the use of UAS for geologic site characterization and change detection studies at a variety of field sites. The deployed UAS equipped with a standard visible band camera to collect imagery datasets. Based on the imagery collected, we use deep sparse algorithmic processing to detect and discriminate subtle topographic features created or impacted by subsurface activities. In this work, we develop an object-oriented remote sensing imagery clustering method for land cover classification. To improve the clustering and segmentation accuracy, instead of using conventional pixel-based clustering methods, we integrate the spatial information from neighboring regions to create super-pixels to avoid salt-and-pepper noise and subsequent over-segmentation. To further improve robustness of our clustering method, we also incorporate a custom digital elevation model (DEM) dataset generated using a structure-from-motion (SfM) algorithm together with the red, green, and blue (RGB) band data for clustering. In particular, we first employ an agglomerative clustering to create an initial segmentation map, from where every object is treated as a single (new) pixel. Based on the new pixels obtained, we generate new features to implement another level of clustering. We employ our clustering method to the RGB+DEM datasets collected at the field site. Through binary clustering and multi-object clustering tests, we verify that our method can accurately separate vegetation from non-vegetation regions, and are also able to differentiate object features on the surface.

  1. Object-oriented design of medical imaging software.

    PubMed

    Ligier, Y; Ratib, O; Logean, M; Girard, C; Perrier, R; Scherrer, J R

    1994-01-01

    A special software package for interactive display and manipulation of medical images was developed at the University Hospital of Geneva, as part of a hospital wide Picture Archiving and Communication System (PACS). This software package, called Osiris, was especially designed to be easily usable and adaptable to the needs of noncomputer-oriented physicians. The Osiris software has been developed to allow the visualization of medical images obtained from any imaging modality. It provides generic manipulation tools, processing tools, and analysis tools more specific to clinical applications. This software, based on an object-oriented paradigm, is portable and extensible. Osiris is available on two different operating systems: the Unix X-11/OSF-Motif based workstations, and the Macintosh family.

  2. Einstein-Podolsky-Rosen paradox in single pairs of images.

    PubMed

    Lantz, Eric; Denis, Séverine; Moreau, Paul-Antoine; Devaux, Fabrice

    2015-10-05

    Spatially entangled twin photons provide a test of the Einstein-Podolsky-Rosen (EPR) paradox in its original form of position (image plane) versus impulsion (Fourier plane). We show that recording a single pair of images in each plane is sufficient to safely demonstrate an EPR paradox. On each pair of images, we have retrieved the fluctuations by subtracting the fitted deterministic intensity shape and then have obtained an intercorrelation peak with a sufficient signal to noise ratio to safely distinguish this peak from random fluctuations. A 95% confidence interval has been determined, confirming a high degree of paradox whatever the considered single pairs. Last, we have verified that the value of the variance of the difference between twin images is always below the quantum (poissonian) limit, in order to ensure the particle character of the demonstration. Our demonstration shows that a single image pattern can reveal the quantum and non-local behavior of light.

  3. Lagrangian 3D tracking of fluorescent microscopic objects in motion

    NASA Astrophysics Data System (ADS)

    Darnige, T.; Figueroa-Morales, N.; Bohec, P.; Lindner, A.; Clément, E.

    2017-05-01

    We describe the development of a tracking device, mounted on an epi-fluorescent inverted microscope, suited to obtain time resolved 3D Lagrangian tracks of fluorescent passive or active micro-objects in microfluidic devices. The system is based on real-time image processing, determining the displacement of a x, y mechanical stage to keep the chosen object at a fixed position in the observation frame. The z displacement is based on the refocusing of the fluorescent object determining the displacement of a piezo mover keeping the moving object in focus. Track coordinates of the object with respect to the microfluidic device as well as images of the object are obtained at a frequency of several tenths of Hertz. This device is particularly well adapted to obtain trajectories of motile micro-organisms in microfluidic devices with or without flow.

  4. Lagrangian 3D tracking of fluorescent microscopic objects in motion.

    PubMed

    Darnige, T; Figueroa-Morales, N; Bohec, P; Lindner, A; Clément, E

    2017-05-01

    We describe the development of a tracking device, mounted on an epi-fluorescent inverted microscope, suited to obtain time resolved 3D Lagrangian tracks of fluorescent passive or active micro-objects in microfluidic devices. The system is based on real-time image processing, determining the displacement of a x, y mechanical stage to keep the chosen object at a fixed position in the observation frame. The z displacement is based on the refocusing of the fluorescent object determining the displacement of a piezo mover keeping the moving object in focus. Track coordinates of the object with respect to the microfluidic device as well as images of the object are obtained at a frequency of several tenths of Hertz. This device is particularly well adapted to obtain trajectories of motile micro-organisms in microfluidic devices with or without flow.

  5. Active illuminated space object imaging and tracking simulation

    NASA Astrophysics Data System (ADS)

    Yue, Yufang; Xie, Xiaogang; Luo, Wen; Zhang, Feizhou; An, Jianzhu

    2016-10-01

    Optical earth imaging simulation of a space target in orbit and it's extraction in laser illumination condition were discussed. Based on the orbit and corresponding attitude of a satellite, its 3D imaging rendering was built. General simulation platform was researched, which was adaptive to variable 3D satellite models and relative position relationships between satellite and earth detector system. Unified parallel projection technology was proposed in this paper. Furthermore, we denoted that random optical distribution in laser-illuminated condition was a challenge for object discrimination. Great randomicity of laser active illuminating speckles was the primary factor. The conjunction effects of multi-frame accumulation process and some tracking methods such as Meanshift tracking, contour poid, and filter deconvolution were simulated. Comparison of results illustrates that the union of multi-frame accumulation and contour poid was recommendable for laser active illuminated images, which had capacities of high tracking precise and stability for multiple object attitudes.

  6. Application of a single-objective, hybrid genetic algorithm approach to pharmacokinetic model building.

    PubMed

    Sherer, Eric A; Sale, Mark E; Pollock, Bruce G; Belani, Chandra P; Egorin, Merrill J; Ivy, Percy S; Lieberman, Jeffrey A; Manuck, Stephen B; Marder, Stephen R; Muldoon, Matthew F; Scher, Howard I; Solit, David B; Bies, Robert R

    2012-08-01

    A limitation in traditional stepwise population pharmacokinetic model building is the difficulty in handling interactions between model components. To address this issue, a method was previously introduced which couples NONMEM parameter estimation and model fitness evaluation to a single-objective, hybrid genetic algorithm for global optimization of the model structure. In this study, the generalizability of this approach for pharmacokinetic model building is evaluated by comparing (1) correct and spurious covariate relationships in a simulated dataset resulting from automated stepwise covariate modeling, Lasso methods, and single-objective hybrid genetic algorithm approaches to covariate identification and (2) information criteria values, model structures, convergence, and model parameter values resulting from manual stepwise versus single-objective, hybrid genetic algorithm approaches to model building for seven compounds. Both manual stepwise and single-objective, hybrid genetic algorithm approaches to model building were applied, blinded to the results of the other approach, for selection of the compartment structure as well as inclusion and model form of inter-individual and inter-occasion variability, residual error, and covariates from a common set of model options. For the simulated dataset, stepwise covariate modeling identified three of four true covariates and two spurious covariates; Lasso identified two of four true and 0 spurious covariates; and the single-objective, hybrid genetic algorithm identified three of four true covariates and one spurious covariate. For the clinical datasets, the Akaike information criterion was a median of 22.3 points lower (range of 470.5 point decrease to 0.1 point decrease) for the best single-objective hybrid genetic-algorithm candidate model versus the final manual stepwise model: the Akaike information criterion was lower by greater than 10 points for four compounds and differed by less than 10 points for three

  7. Objective Quality Assessment for Color-to-Gray Image Conversion.

    PubMed

    Ma, Kede; Zhao, Tiesong; Zeng, Kai; Wang, Zhou

    2015-12-01

    Color-to-gray (C2G) image conversion is the process of transforming a color image into a grayscale one. Despite its wide usage in real-world applications, little work has been dedicated to compare the performance of C2G conversion algorithms. Subjective evaluation is reliable but is also inconvenient and time consuming. Here, we make one of the first attempts to develop an objective quality model that automatically predicts the perceived quality of C2G converted images. Inspired by the philosophy of the structural similarity index, we propose a C2G structural similarity (C2G-SSIM) index, which evaluates the luminance, contrast, and structure similarities between the reference color image and the C2G converted image. The three components are then combined depending on image type to yield an overall quality measure. Experimental results show that the proposed C2G-SSIM index has close agreement with subjective rankings and significantly outperforms existing objective quality metrics for C2G conversion. To explore the potentials of C2G-SSIM, we further demonstrate its use in two applications: 1) automatic parameter tuning for C2G conversion algorithms and 2) adaptive fusion of C2G converted images.

  8. Calibration for single multi-mode fiber digital scanning microscopy imaging system

    NASA Astrophysics Data System (ADS)

    Yin, Zhe; Liu, Guodong; Liu, Bingguo; Gan, Yu; Zhuang, Zhitao; Chen, Fengdong

    2015-11-01

    Single multimode fiber (MMF) digital scanning imaging system is a development tendency of modern endoscope. We concentrate on the calibration method of the imaging system. Calibration method comprises two processes, forming scanning focused spots and calibrating the couple factors varied with positions. Adaptive parallel coordinate algorithm (APC) is adopted to form the focused spots at the multimode fiber (MMF) output. Compare with other algorithm, APC contains many merits, i.e. rapid speed, small amount calculations and no iterations. The ratio of the optics power captured by MMF to the intensity of the focused spots is called couple factor. We setup the calibration experimental system to form the scanning focused spots and calculate the couple factors for different object positions. The experimental result the couple factor is higher in the center than the edge.

  9. Single-Molecule Spectroscopy and Imaging Over the Decades

    PubMed Central

    Moerner, W. E.; Shechtman, Yoav; Wang, Quan

    2016-01-01

    As of 2015, it has been 26 years since the first optical detection and spectroscopy of single molecules in condensed matter. This area of science has expanded far beyond the early low temperature studies in crystals to include single molecules in cells, polymers, and in solution. The early steps relied upon high-resolution spectroscopy of inhomogeneously broadened optical absorption profiles of molecular impurities in solids at low temperatures. Spectral fine structure arising directly from the position-dependent fluctuations of the number of molecules in resonance led to the attainment of the single-molecule limit in 1989 using frequency-modulation laser spectroscopy. In the early 1990's, a variety of fascinating physical effects were observed for individual molecules, including imaging of the light from single molecules as well as observations of spectral diffusion, optical switching and the ability to select different single molecules in the same focal volume simply by tuning the pumping laser frequency. In the room temperature regime, researchers showed that bursts of light from single molecules could be detected in solution, leading to imaging and microscopy by a variety of methods. Studies of single copies of the green fluorescent protein also uncovered surprises, especially the blinking and photoinduced recovery of emitters, which stimulated further development of photoswitchable fluorescent protein labels. All of these early steps provided important fundamentals underpinning the development of super-resolution microscopy based on single-molecule localization and active control of emitting concentration. Current thrust areas include extensions to three-dimensional imaging with high precision, orientational analysis of single molecules, and direct measurements of photodynamics and transport properties for single molecules trapped in solution by suppression of Brownian motion. Without question, a huge variety of studies of single molecules performed by many

  10. Measuring the circular motion of small objects using laser stroboscopic images.

    PubMed

    Wang, Hairong; Fu, Y; Du, R

    2008-01-01

    Measuring the circular motion of a small object, including its displacement, speed, and acceleration, is a challenging task. This paper presents a new method for measuring repetitive and/or nonrepetitive, constant speed and/or variable speed circular motion using laser stroboscopic images. Under stroboscopic illumination, each image taken by an ordinary camera records multioutlines of an object in motion; hence, processing the stroboscopic image will be able to extract the motion information. We built an experiment apparatus consisting of a laser as the light source, a stereomicroscope to magnify the image, and a normal complementary metal oxide semiconductor camera to record the image. As the object is in motion, the stroboscopic illumination generates a speckle pattern on the object that can be recorded by the camera and analyzed by a computer. Experimental results indicate that the stroboscopic imaging is stable under various conditions. Moreover, the characteristics of the motion, including the displacement, the velocity, and the acceleration can be calculated based on the width of speckle marks, the illumination intensity, the duty cycle, and the sampling frequency. Compared with the popular high-speed camera method, the presented method may achieve the same measuring accuracy, but with much reduced cost and complexity.

  11. Intranucleus Single-Molecule Imaging in Living Cells.

    PubMed

    Shao, Shipeng; Xue, Boxin; Sun, Yujie

    2018-06-01

    Many critical processes occurring in mammalian cells are stochastic and can be directly observed at the single-molecule level within their physiological environment, which would otherwise be obscured in an ensemble measurement. There are various fundamental processes in the nucleus, such as transcription, replication, and DNA repair, the study of which can greatly benefit from intranuclear single-molecule imaging. However, the number of such studies is relatively small mainly because of lack of proper labeling and imaging methods. In the past decade, tremendous efforts have been devoted to developing tools for intranuclear imaging. Here, we mainly describe the recent methodological developments of single-molecule imaging and their emerging applications in the live nucleus. We also discuss the remaining issues and provide a perspective on future developments and applications of this field. Copyright © 2018 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  12. Identification of simple objects in image sequences

    NASA Astrophysics Data System (ADS)

    Geiselmann, Christoph; Hahn, Michael

    1994-08-01

    We present an investigation in the identification and location of simple objects in color image sequences. As an example the identification of traffic signs is discussed. Three aspects are of special interest. First regions have to be detected which may contain the object. The separation of those regions from the background can be based on color, motion, and contours. In the experiments all three possibilities are investigated. The second aspect focuses on the extraction of suitable features for the identification of the objects. For that purpose the border line of the region of interest is used. For planar objects a sufficient approximation of perspective projection is affine mapping. In consequence, it is near at hand to extract affine-invariant features from the border line. The investigation includes invariant features based on Fourier descriptors and moments. Finally, the object is identified by maximum likelihood classification. In the experiments all three basic object types are correctly identified. The probabilities for misclassification have been found to be below 1%

  13. Single-Image Super Resolution for Multispectral Remote Sensing Data Using Convolutional Neural Networks

    NASA Astrophysics Data System (ADS)

    Liebel, L.; Körner, M.

    2016-06-01

    In optical remote sensing, spatial resolution of images is crucial for numerous applications. Space-borne systems are most likely to be affected by a lack of spatial resolution, due to their natural disadvantage of a large distance between the sensor and the sensed object. Thus, methods for single-image super resolution are desirable to exceed the limits of the sensor. Apart from assisting visual inspection of datasets, post-processing operations—e.g., segmentation or feature extraction—can benefit from detailed and distinguishable structures. In this paper, we show that recently introduced state-of-the-art approaches for single-image super resolution of conventional photographs, making use of deep learning techniques, such as convolutional neural networks (CNN), can successfully be applied to remote sensing data. With a huge amount of training data available, end-to-end learning is reasonably easy to apply and can achieve results unattainable using conventional handcrafted algorithms. We trained our CNN on a specifically designed, domain-specific dataset, in order to take into account the special characteristics of multispectral remote sensing data. This dataset consists of publicly available SENTINEL-2 images featuring 13 spectral bands, a ground resolution of up to 10m, and a high radiometric resolution and thus satisfying our requirements in terms of quality and quantity. In experiments, we obtained results superior compared to competing approaches trained on generic image sets, which failed to reasonably scale satellite images with a high radiometric resolution, as well as conventional interpolation methods.

  14. Artist's Concept of 2014 MU69 as a Single Object

    NASA Image and Video Library

    2017-08-03

    Artist's concept of Kuiper Belt object 2014 MU69, which is the next flyby target for NASA's New Horizons mission. Scientists speculate that the Kuiper Belt object could be a single body (above) with a large chunk taken out of it, or two bodies that are close together or even touching. https://photojournal.jpl.nasa.gov/catalog/PIA21868

  15. Simulations of Liners and Test Objects for a New Atlas Advanced Radiography Source

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    D. V. Morgan; S. Iversen; R. A. Hilko

    2002-06-01

    The Advanced Radiographic Source (ARS) will improve the data significantly due to its smaller source width. Because of the enhanced ARS output, larger source-to-object distances are a reality. The harder ARS source will allow radiography of thick high-Z targets. The five different spectral simulations resulted in similar imaging detector weighted transmission. This work used a limited set of test objects and imaging detectors. Other test objects and imaging detectors could possibly change the MVp-sensitivity result. The effect of material motion blur must be considered for the ARS due to the expected smaller X-ray source size. This study supports the originalmore » 1.5-MVp value.« less

  16. Single-shot magnetic resonance spectroscopic imaging with partial parallel imaging.

    PubMed

    Posse, Stefan; Otazo, Ricardo; Tsai, Shang-Yueh; Yoshimoto, Akio Ernesto; Lin, Fa-Hsuan

    2009-03-01

    A magnetic resonance spectroscopic imaging (MRSI) pulse sequence based on proton-echo-planar-spectroscopic-imaging (PEPSI) is introduced that measures two-dimensional metabolite maps in a single excitation. Echo-planar spatial-spectral encoding was combined with interleaved phase encoding and parallel imaging using SENSE to reconstruct absorption mode spectra. The symmetrical k-space trajectory compensates phase errors due to convolution of spatial and spectral encoding. Single-shot MRSI at short TE was evaluated in phantoms and in vivo on a 3-T whole-body scanner equipped with a 12-channel array coil. Four-step interleaved phase encoding and fourfold SENSE acceleration were used to encode a 16 x 16 spatial matrix with a 390-Hz spectral width. Comparison with conventional PEPSI and PEPSI with fourfold SENSE acceleration demonstrated comparable sensitivity per unit time when taking into account g-factor-related noise increases and differences in sampling efficiency. LCModel fitting enabled quantification of inositol, choline, creatine, and N-acetyl-aspartate (NAA) in vivo with concentration values in the ranges measured with conventional PEPSI and SENSE-accelerated PEPSI. Cramer-Rao lower bounds were comparable to those obtained with conventional SENSE-accelerated PEPSI at the same voxel size and measurement time. This single-shot MRSI method is therefore suitable for applications that require high temporal resolution to monitor temporal dynamics or to reduce sensitivity to tissue movement.

  17. Computational efficient unsupervised coastline detection from single-polarization 1-look SAR images of complex coastal environments

    NASA Astrophysics Data System (ADS)

    Garzelli, Andrea; Zoppetti, Claudia; Pinelli, Gianpaolo

    2017-10-01

    Coastline detection in synthetic aperture radar (SAR) images is crucial in many application fields, from coastal erosion monitoring to navigation, from damage assessment to security planning for port facilities. The backscattering difference between land and sea is not always documented in SAR imagery, due to the severe speckle noise, especially in 1-look data with high spatial resolution, high sea state, or complex coastal environments. This paper presents an unsupervised, computationally efficient solution to extract the coastline acquired by only one single-polarization 1-look SAR image. Extensive tests on Spotlight COSMO-SkyMed images of complex coastal environments and objective assessment demonstrate the validity of the proposed procedure which is compared to state-of-the-art methods through visual results and with an objective evaluation of the distance between the detected and the true coastline provided by regional authorities.

  18. Adaptive single-pixel imaging with aggregated sampling and continuous differential measurements

    NASA Astrophysics Data System (ADS)

    Huo, Yaoran; He, Hongjie; Chen, Fan; Tai, Heng-Ming

    2018-06-01

    This paper proposes an adaptive compressive imaging technique with one single-pixel detector and single arm. The aggregated sampling (AS) method enables the reduction of resolutions of the reconstructed images. It aims to reduce the time and space consumption. The target image with a resolution up to 1024 × 1024 can be reconstructed successfully at the 20% sampling rate. The continuous differential measurement (CDM) method combined with a ratio factor of significant coefficient (RFSC) improves the imaging quality. Moreover, RFSC reduces the human intervention in parameter setting. This technique enhances the practicability of single-pixel imaging with the benefits from less time and space consumption, better imaging quality and less human intervention.

  19. Cryo-imaging of fluorescently labeled single cells in a mouse

    NASA Astrophysics Data System (ADS)

    Steyer, Grant J.; Roy, Debashish; Salvado, Olivier; Stone, Meredith E.; Wilson, David L.

    2009-02-01

    We developed a cryo-imaging system to provide single-cell detection of fluorescently labeled cells in mouse, with particular applicability to stem cells and metastatic cancer. The Case cryoimaging system consists of a fluorescence microscope, robotic imaging positioner, customized cryostat, PC-based control system, and visualization/analysis software. The system alternates between sectioning (10-40 μm) and imaging, collecting color brightfield and fluorescent blockface image volumes >60GB. In mouse experiments, we imaged quantum-dot labeled stem cells, GFP-labeled cancer and stem cells, and cell-size fluorescent microspheres. To remove subsurface fluorescence, we used a simplified model of light-tissue interaction whereby the next image was scaled, blurred, and subtracted from the current image. We estimated scaling and blurring parameters by minimizing entropy of subtracted images. Tissue specific attenuation parameters were found [uT : heart (267 +/- 47.6 μm), liver (218 +/- 27.1 μm), brain (161 +/- 27.4 μm)] to be within the range of estimates in the literature. "Next image" processing removed subsurface fluorescence equally well across multiple tissues (brain, kidney, liver, adipose tissue, etc.), and analysis of 200 microsphere images in the brain gave 97+/-2% reduction of subsurface fluorescence. Fluorescent signals were determined to arise from single cells based upon geometric and integrated intensity measurements. Next image processing greatly improved axial resolution, enabled high quality 3D volume renderings, and improved enumeration of single cells with connected component analysis by up to 24%. Analysis of image volumes identified metastatic cancer sites, found homing of stem cells to injury sites, and showed microsphere distribution correlated with blood flow patterns. We developed and evaluated cryo-imaging to provide single-cell detection of fluorescently labeled cells in mouse. Our cryo-imaging system provides extreme (>60GB), micron

  20. Graphical user interface to optimize image contrast parameters used in object segmentation - biomed 2009.

    PubMed

    Anderson, Jeffrey R; Barrett, Steven F

    2009-01-01

    Image segmentation is the process of isolating distinct objects within an image. Computer algorithms have been developed to aid in the process of object segmentation, but a completely autonomous segmentation algorithm has yet to be developed [1]. This is because computers do not have the capability to understand images and recognize complex objects within the image. However, computer segmentation methods [2], requiring user input, have been developed to quickly segment objects in serial sectioned images, such as magnetic resonance images (MRI) and confocal laser scanning microscope (CLSM) images. In these cases, the segmentation process becomes a powerful tool in visualizing the 3D nature of an object. The user input is an important part of improving the performance of many segmentation methods. A double threshold segmentation method has been investigated [3] to separate objects in gray scaled images, where the gray level of the object is among the gray levels of the background. In order to best determine the threshold values for this segmentation method the image must be manipulated for optimal contrast. The same is true of other segmentation and edge detection methods as well. Typically, the better the image contrast, the better the segmentation results. This paper describes a graphical user interface (GUI) that allows the user to easily change image contrast parameters that will optimize the performance of subsequent object segmentation. This approach makes use of the fact that the human brain is extremely effective in object recognition and understanding. The GUI provides the user with the ability to define the gray scale range of the object of interest. These lower and upper bounds of this range are used in a histogram stretching process to improve image contrast. Also, the user can interactively modify the gamma correction factor that provides a non-linear distribution of gray scale values, while observing the corresponding changes to the image. This

  1. CMEIAS color segmentation: an improved computing technology to process color images for quantitative microbial ecology studies at single-cell resolution.

    PubMed

    Gross, Colin A; Reddy, Chandan K; Dazzo, Frank B

    2010-02-01

    Quantitative microscopy and digital image analysis are underutilized in microbial ecology largely because of the laborious task to segment foreground object pixels from background, especially in complex color micrographs of environmental samples. In this paper, we describe an improved computing technology developed to alleviate this limitation. The system's uniqueness is its ability to edit digital images accurately when presented with the difficult yet commonplace challenge of removing background pixels whose three-dimensional color space overlaps the range that defines foreground objects. Image segmentation is accomplished by utilizing algorithms that address color and spatial relationships of user-selected foreground object pixels. Performance of the color segmentation algorithm evaluated on 26 complex micrographs at single pixel resolution had an overall pixel classification accuracy of 99+%. Several applications illustrate how this improved computing technology can successfully resolve numerous challenges of complex color segmentation in order to produce images from which quantitative information can be accurately extracted, thereby gain new perspectives on the in situ ecology of microorganisms. Examples include improvements in the quantitative analysis of (1) microbial abundance and phylotype diversity of single cells classified by their discriminating color within heterogeneous communities, (2) cell viability, (3) spatial relationships and intensity of bacterial gene expression involved in cellular communication between individual cells within rhizoplane biofilms, and (4) biofilm ecophysiology based on ribotype-differentiated radioactive substrate utilization. The stand-alone executable file plus user manual and tutorial images for this color segmentation computing application are freely available at http://cme.msu.edu/cmeias/ . This improved computing technology opens new opportunities of imaging applications where discriminating colors really matter most

  2. High-resolution single photon planar and spect imaging of brain and neck employing a system of two co-registered opposed gamma imaging heads

    DOEpatents

    Majewski, Stanislaw [Yorktown, VA; Proffitt, James [Newport News, VA

    2011-12-06

    A compact, mobile, dedicated SPECT brain imager that can be easily moved to the patient to provide in-situ imaging, especially when the patient cannot be moved to the Nuclear Medicine imaging center. As a result of the widespread availability of single photon labeled biomarkers, the SPECT brain imager can be used in many locations, including remote locations away from medical centers. The SPECT imager improves the detection of gamma emission from the patient's head and neck area with a large field of view. Two identical lightweight gamma imaging detector heads are mounted to a rotating gantry and precisely mechanically co-registered to each other at 180 degrees. A unique imaging algorithm combines the co-registered images from the detector heads and provides several SPECT tomographic reconstructions of the imaged object thereby improving the diagnostic quality especially in the case of imaging requiring higher spatial resolution and sensitivity at the same time.

  3. Method and apparatus for detecting internal structures of bulk objects using acoustic imaging

    DOEpatents

    Deason, Vance A.; Telschow, Kenneth L.

    2002-01-01

    Apparatus for producing an acoustic image of an object according to the present invention may comprise an excitation source for vibrating the object to produce at least one acoustic wave therein. The acoustic wave results in the formation of at least one surface displacement on the surface of the object. A light source produces an optical object wavefront and an optical reference wavefront and directs the optical object wavefront toward the surface of the object to produce a modulated optical object wavefront. A modulator operatively associated with the optical reference wavefront modulates the optical reference wavefront in synchronization with the acoustic wave to produce a modulated optical reference wavefront. A sensing medium positioned to receive the modulated optical object wavefront and the modulated optical reference wavefront combines the modulated optical object and reference wavefronts to produce an image related to the surface displacement on the surface of the object. A detector detects the image related to the surface displacement produced by the sensing medium. A processing system operatively associated with the detector constructs an acoustic image of interior features of the object based on the phase and amplitude of the surface displacement on the surface of the object.

  4. Model predictive direct power control for active power decoupled single-phase quasi- Z -source inverter

    DOE PAGES

    Liu, Yushan; Ge, Baoming; Abu-Rub, Haitham; ...

    2016-06-14

    In this study, the active power filter (APF) that consists of a half-bridge leg and an ac capacitor is integrated in the single-phase quasi-Z-source inverter (qZSI) in this paper to avoid the second harmonic power flowing into the dc side. The capacitor of APF buffers the second harmonic power of the load, and the ac capacitor allows highly pulsating ac voltage, so that the capacitances of both dc and ac sides can be small. A model predictive direct power control (DPC) is further proposed to achieve the purpose of this newtopology through predicting the capacitor voltage of APF at eachmore » sampling period and ensuring the APF power to track the second harmonic power of single-phase qZSI. Simulation and experimental results verify the model predictive DPC for the APF-integrated single-phase qZSI.« less

  5. Model predictive direct power control for active power decoupled single-phase quasi- Z -source inverter

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Liu, Yushan; Ge, Baoming; Abu-Rub, Haitham

    In this study, the active power filter (APF) that consists of a half-bridge leg and an ac capacitor is integrated in the single-phase quasi-Z-source inverter (qZSI) in this paper to avoid the second harmonic power flowing into the dc side. The capacitor of APF buffers the second harmonic power of the load, and the ac capacitor allows highly pulsating ac voltage, so that the capacitances of both dc and ac sides can be small. A model predictive direct power control (DPC) is further proposed to achieve the purpose of this newtopology through predicting the capacitor voltage of APF at eachmore » sampling period and ensuring the APF power to track the second harmonic power of single-phase qZSI. Simulation and experimental results verify the model predictive DPC for the APF-integrated single-phase qZSI.« less

  6. Image Engine: an object-oriented multimedia database for storing, retrieving and sharing medical images and text.

    PubMed Central

    Lowe, H. J.

    1993-01-01

    This paper describes Image Engine, an object-oriented, microcomputer-based, multimedia database designed to facilitate the storage and retrieval of digitized biomedical still images, video, and text using inexpensive desktop computers. The current prototype runs on Apple Macintosh computers and allows network database access via peer to peer file sharing protocols. Image Engine supports both free text and controlled vocabulary indexing of multimedia objects. The latter is implemented using the TView thesaurus model developed by the author. The current prototype of Image Engine uses the National Library of Medicine's Medical Subject Headings (MeSH) vocabulary (with UMLS Meta-1 extensions) as its indexing thesaurus. PMID:8130596

  7. Moving object detection using dynamic motion modelling from UAV aerial images.

    PubMed

    Saif, A F M Saifuddin; Prabuwono, Anton Satria; Mahayuddin, Zainal Rasyid

    2014-01-01

    Motion analysis based moving object detection from UAV aerial image is still an unsolved issue due to inconsideration of proper motion estimation. Existing moving object detection approaches from UAV aerial images did not deal with motion based pixel intensity measurement to detect moving object robustly. Besides current research on moving object detection from UAV aerial images mostly depends on either frame difference or segmentation approach separately. There are two main purposes for this research: firstly to develop a new motion model called DMM (dynamic motion model) and secondly to apply the proposed segmentation approach SUED (segmentation using edge based dilation) using frame difference embedded together with DMM model. The proposed DMM model provides effective search windows based on the highest pixel intensity to segment only specific area for moving object rather than searching the whole area of the frame using SUED. At each stage of the proposed scheme, experimental fusion of the DMM and SUED produces extracted moving objects faithfully. Experimental result reveals that the proposed DMM and SUED have successfully demonstrated the validity of the proposed methodology.

  8. GuidosToolbox: universal digital image object analysis

    Treesearch

    Peter Vogt; Kurt Riitters

    2017-01-01

    The increased availability of mapped environmental data calls for better tools to analyze the spatial characteristics and information contained in those maps. Publicly available, userfriendly and universal tools are needed to foster the interdisciplinary development and application of methodologies for the extraction of image object information properties contained in...

  9. Object Recognition and Random Image Structure Evolution

    ERIC Educational Resources Information Center

    Sadr, Jvid; Sinha, Pawan

    2004-01-01

    We present a technique called Random Image Structure Evolution (RISE) for use in experimental investigations of high-level visual perception. Potential applications of RISE include the quantitative measurement of perceptual hysteresis and priming, the study of the neural substrates of object perception, and the assessment and detection of subtle…

  10. Real-time system for imaging and object detection with a multistatic GPR array

    DOEpatents

    Paglieroni, David W; Beer, N Reginald; Bond, Steven W; Top, Philip L; Chambers, David H; Mast, Jeffrey E; Donetti, John G; Mason, Blake C; Jones, Steven M

    2014-10-07

    A method and system for detecting the presence of subsurface objects within a medium is provided. In some embodiments, the imaging and detection system operates in a multistatic mode to collect radar return signals generated by an array of transceiver antenna pairs that is positioned across the surface and that travels down the surface. The imaging and detection system pre-processes the return signal to suppress certain undesirable effects. The imaging and detection system then generates synthetic aperture radar images from real aperture radar images generated from the pre-processed return signal. The imaging and detection system then post-processes the synthetic aperture radar images to improve detection of subsurface objects. The imaging and detection system identifies peaks in the energy levels of the post-processed image frame, which indicates the presence of a subsurface object.

  11. Dynamic granularity of imaging systems

    DOE PAGES

    Geissel, Matthias; Smith, Ian C.; Shores, Jonathon E.; ...

    2015-11-04

    Imaging systems that include a specific source, imaging concept, geometry, and detector have unique properties such as signal-to-noise ratio, dynamic range, spatial resolution, distortions, and contrast. Some of these properties are inherently connected, particularly dynamic range and spatial resolution. It must be emphasized that spatial resolution is not a single number but must be seen in the context of dynamic range and consequently is better described by a function or distribution. We introduce the “dynamic granularity” G dyn as a standardized, objective relation between a detector’s spatial resolution (granularity) and dynamic range for complex imaging systems in a given environmentmore » rather than the widely found characterization of detectors such as cameras or films by themselves. We found that this relation can partly be explained through consideration of the signal’s photon statistics, background noise, and detector sensitivity, but a comprehensive description including some unpredictable data such as dust, damages, or an unknown spectral distribution will ultimately have to be based on measurements. Measured dynamic granularities can be objectively used to assess the limits of an imaging system’s performance including all contributing noise sources and to qualify the influence of alternative components within an imaging system. Our article explains the construction criteria to formulate a dynamic granularity and compares measured dynamic granularities for different detectors used in the X-ray backlighting scheme employed at Sandia’s Z-Backlighter facility.« less

  12. Frontier Fields: Combining HST, VLT, and Spitzer data to explore the z ~ 8 Universe behind the lensing cluster MACSJ0416.1-2403

    NASA Astrophysics Data System (ADS)

    Laporte, N.; Streblyanska, A.; Kim, S.; Pelló, R.; Bauer, F. E.; Bina, D.; Brammer, G.; De Leo, M. A.; Infante, L.; Pérez-Fournon, I.

    2015-03-01

    Context. The Hubble Space Telescope (HST) Frontier Fields (HFFs) project started at the end of 2013 with the aim of providing extremely deep images of six massive galaxy clusters. One of the main goals of this program is to push several telescopes to their limits to provide the best current view of the earliest stages of the Universe. The analysis of the initial data has already demonstrated the huge capabilities of the program. Aims: We present a detailed analysis of z ~ 8 objects behind the HFFs lensing cluster, MACSJ0416.1-2403, combining 0.3-1.6 μm imaging from HST, ground-based Ks imaging from VLT HAWK-I, and 3.6 μm and 4.5 μm Spitzer Space Telescope. The images probe to 5σ depths of ≈29 AB for HST, 25.6 AB for HAWK-I, and ≈0.310 and 0.391 μJy at 3.6 and 4.5 μm, respectively. With these datasets, we assess the photometric properties of z ~ 8 galaxies in this field, as well as their distribution in luminosity, to unprecedented sensitivity. Methods: We applied the classical Lyman break (LB) technique, which combines non detection criteria in bands blueward of the Lyman break at z ~ 8 and color-selection in bands redward of the break. To avoid contamination by mid-z interlopers, we required a strong break between optical and near-infrared data. We determined the photometric properties of z ~ 8 selected candidates using spectral energy distribution (SED)-fitting with standard library templates. The luminosity function at z ~ 8 is computed using a Monte-Carlo method taking advantage of the SED-fitting results. A piece of cautionary information is gleaned from new deep optical photometry of a previously identified z ~ 8 galaxy in this cluster, which is now firmly detected as a mid-z interloper with a strong ≈1.5 mag Balmer break (between F606W and F125W). Using the SED of this interloper, we estimated the contamination rate of our MACSJ0416.1-2403 sample, and that of previous samples in Abell 2744 that were based on HFF data, we highlight the dangers of

  13. Mobile object retrieval in server-based image databases

    NASA Astrophysics Data System (ADS)

    Manger, D.; Pagel, F.; Widak, H.

    2013-05-01

    The increasing number of mobile phones equipped with powerful cameras leads to huge collections of user-generated images. To utilize the information of the images on site, image retrieval systems are becoming more and more popular to search for similar objects in an own image database. As the computational performance and the memory capacity of mobile devices are constantly increasing, this search can often be performed on the device itself. This is feasible, for example, if the images are represented with global image features or if the search is done using EXIF or textual metadata. However, for larger image databases, if multiple users are meant to contribute to a growing image database or if powerful content-based image retrieval methods with local features are required, a server-based image retrieval backend is needed. In this work, we present a content-based image retrieval system with a client server architecture working with local features. On the server side, the scalability to large image databases is addressed with the popular bag-of-word model with state-of-the-art extensions. The client end of the system focuses on a lightweight user interface presenting the most similar images of the database highlighting the visual information which is common with the query image. Additionally, new images can be added to the database making it a powerful and interactive tool for mobile contentbased image retrieval.

  14. IDL Object Oriented Software for Hinode/XRT Image Analysis

    NASA Astrophysics Data System (ADS)

    Higgins, P. A.; Gallagher, P. T.

    2008-09-01

    We have developed a set of object oriented IDL routines that enable users to search, download and analyse images from the X-Ray Telescope (XRT) on-board Hinode. In this paper, we give specific examples of how the object can be used and how multi-instrument data analysis can be performed. The XRT object is a highly versatile and powerful IDL object, which will prove to be a useful tool for solar researchers. This software utilizes the generic Framework object available within the GEN branch of SolarSoft.

  15. Imaging discrepancies between magnetic resonance imaging and brain perfusion single-photon emission computed tomography in the diagnosis of Alzheimer's disease, and verification with amyloid positron emission tomography.

    PubMed

    Yokoyama, Shunichi; Kajiya, Yoriko; Yoshinaga, Takuma; Tani, Atsushi; Hirano, Hirofumi

    2014-06-01

    In the diagnosis of Alzheimer's disease (AD), discrepancies are often observed between magnetic resonance imaging (MRI) and brain perfusion single-photon emission computed tomography (SPECT) findings. MRI, brain perfusion SPECT, and amyloid positron emission tomography (PET) findings were compared in patients with mild cognitive impairment or early AD to clarify the discrepancies between imaging modalities. Several imaging markers were investigated, including the cortical average standardized uptake value ratio on amyloid PET, the Z-score of a voxel-based specific regional analysis system for AD on MRI, periventricular hyperintensity grade, deep white matter hyperintense signal grade, number of microbleeds, and three indicators of the easy Z-score imaging system for a specific SPECT volume-of-interest analysis. Based on the results of the regional analysis and the three indicators, we classified patients into four groups and then compared the results of amyloid PET, periventricular hyperintensity grade, deep white matter hyperintense signal grade, and the numbers of microbleeds among the groups. The amyloid deposition was the highest in the group that presented typical AD findings on both the regional analysis and the three indicators. The two groups that showed an imaging discrepancy between the regional analysis and the three indicators demonstrated intermediate amyloid deposition findings compared with the typical and atypical groups. The patients who showed hippocampal atrophy on the regional analysis and atypical AD findings using the three indicators were approximately 60% amyloid-negative. The mean periventricular hyperintensity grade was highest in the typical group. Patients showing discrepancies between MRI and SPECT demonstrated intermediate amyloid deposition findings compared with patients who showed typical or atypical findings. Strong white matter signal abnormalities on MRI in patients who presented typical AD findings provided further evidence for

  16. Single exposure three-dimensional imaging of dusty plasma clusters.

    PubMed

    Hartmann, Peter; Donkó, István; Donkó, Zoltán

    2013-02-01

    We have worked out the details of a single camera, single exposure method to perform three-dimensional imaging of a finite particle cluster. The procedure is based on the plenoptic imaging principle and utilizes a commercial Lytro light field still camera. We demonstrate the capabilities of our technique on a single layer particle cluster in a dusty plasma, where the camera is aligned and inclined at a small angle to the particle layer. The reconstruction of the third coordinate (depth) is found to be accurate and even shadowing particles can be identified.

  17. Adaptive Morphological Feature-Based Object Classifier for a Color Imaging System

    NASA Technical Reports Server (NTRS)

    McDowell, Mark; Gray, Elizabeth

    2009-01-01

    Utilizing a Compact Color Microscope Imaging System (CCMIS), a unique algorithm has been developed that combines human intelligence along with machine vision techniques to produce an autonomous microscope tool for biomedical, industrial, and space applications. This technique is based on an adaptive, morphological, feature-based mapping function comprising 24 mutually inclusive feature metrics that are used to determine the metrics for complex cell/objects derived from color image analysis. Some of the features include: Area (total numbers of non-background pixels inside and including the perimeter), Bounding Box (smallest rectangle that bounds and object), centerX (x-coordinate of intensity-weighted, center-of-mass of an entire object or multi-object blob), centerY (y-coordinate of intensity-weighted, center-of-mass, of an entire object or multi-object blob), Circumference (a measure of circumference that takes into account whether neighboring pixels are diagonal, which is a longer distance than horizontally or vertically joined pixels), . Elongation (measure of particle elongation given as a number between 0 and 1. If equal to 1, the particle bounding box is square. As the elongation decreases from 1, the particle becomes more elongated), . Ext_vector (extremal vector), . Major Axis (the length of a major axis of a smallest ellipse encompassing an object), . Minor Axis (the length of a minor axis of a smallest ellipse encompassing an object), . Partial (indicates if the particle extends beyond the field of view), . Perimeter Points (points that make up a particle perimeter), . Roundness [(4(pi) x area)/perimeter(squared)) the result is a measure of object roundness, or compactness, given as a value between 0 and 1. The greater the ratio, the rounder the object.], . Thin in center (determines if an object becomes thin in the center, (figure-eight-shaped), . Theta (orientation of the major axis), . Smoothness and color metrics for each component (red, green, blue

  18. Images of intravitreal objects projected onto posterior surface of model eye.

    PubMed

    Kawamura, Ryosuke; Shinoda, Kei; Inoue, Makoto; Noda, Toru; Ohnuma, Kazuhiko; Hirakata, Akito

    2013-11-01

    To try to recreate the images reported by patients during vitreous surgery in a model eye. A fluid-filled model eye with a posterior frosted translucent surface which corresponded to the retina was used. Three holes were made in the model eye through which an endoillumination pipe and intraocular forceps could be inserted. A thin plastic sheet simulating an epiretinal membrane and an intraocular lens (IOL) simulating a dislocated IOL were placed on the retina. The images falling on the posterior surface were photographed from the rear. The images seen through the surgical microscope were also recorded. The images from the rear were mirror images of those seen through the surgical microscope. Intraocular instruments were seen as black shafts from the rear. When the plastic sheet was picked up, the tip of the forceps was seen more sharply on the posterior surface. The images of the dislocated IOL from the posterior were similar to that seen through the surgical microscope, including the yellow optics and blue haptics. Intravitreal objects can form images on the surface of a model eye. Objects located closer to the surface are seen more sharply, and the colour of the objects can be identified. © 2013 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

  19. Evidence for Infrared-faint Radio Sources as z > 1 Radio-loud Active Galactic Nuclei

    NASA Astrophysics Data System (ADS)

    Huynh, Minh T.; Norris, Ray P.; Siana, Brian; Middelberg, Enno

    2010-02-01

    Infrared-Faint Radio Sources (IFRSs) are a class of radio objects found in the Australia Telescope Large Area Survey which have no observable mid-infrared counterpart in the Spitzer Wide-area Infrared Extragalactic (SWIRE) survey. The extended Chandra Deep Field South now has even deeper Spitzer imaging (3.6-70 μm) from a number of Legacy surveys. We report the detections of two IFRS sources in IRAC images. The non-detection of two other IFRSs allows us to constrain the source type. Detailed modeling of the spectral energy distribution of these objects shows that they are consistent with high-redshift (z >~ 1) active galactic nuclei.

  20. EVIDENCE FOR INFRARED-FAINT RADIO SOURCES AS z > 1 RADIO-LOUD ACTIVE GALACTIC NUCLEI

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Huynh, Minh T.; Norris, Ray P.; Siana, Brian

    2010-02-10

    Infrared-Faint Radio Sources (IFRSs) are a class of radio objects found in the Australia Telescope Large Area Survey which have no observable mid-infrared counterpart in the Spitzer Wide-area Infrared Extragalactic (SWIRE) survey. The extended Chandra Deep Field South now has even deeper Spitzer imaging (3.6-70 {mu}m) from a number of Legacy surveys. We report the detections of two IFRS sources in IRAC images. The non-detection of two other IFRSs allows us to constrain the source type. Detailed modeling of the spectral energy distribution of these objects shows that they are consistent with high-redshift (z {approx}> 1) active galactic nuclei.

  1. Object-Location-Aware Hashing for Multi-Label Image Retrieval via Automatic Mask Learning.

    PubMed

    Huang, Chang-Qin; Yang, Shang-Ming; Pan, Yan; Lai, Han-Jiang

    2018-09-01

    Learning-based hashing is a leading approach of approximate nearest neighbor search for large-scale image retrieval. In this paper, we develop a deep supervised hashing method for multi-label image retrieval, in which we propose to learn a binary "mask" map that can identify the approximate locations of objects in an image, so that we use this binary "mask" map to obtain length-limited hash codes which mainly focus on an image's objects but ignore the background. The proposed deep architecture consists of four parts: 1) a convolutional sub-network to generate effective image features; 2) a binary "mask" sub-network to identify image objects' approximate locations; 3) a weighted average pooling operation based on the binary "mask" to obtain feature representations and hash codes that pay most attention to foreground objects but ignore the background; and 4) the combination of a triplet ranking loss designed to preserve relative similarities among images and a cross entropy loss defined on image labels. We conduct comprehensive evaluations on four multi-label image data sets. The results indicate that the proposed hashing method achieves superior performance gains over the state-of-the-art supervised or unsupervised hashing baselines.

  2. Femtosecond few- to single-electron point-projection microscopy for nanoscale dynamic imaging

    PubMed Central

    Bainbridge, A. R.; Barlow Myers, C. W.; Bryan, W. A.

    2016-01-01

    Femtosecond electron microscopy produces real-space images of matter in a series of ultrafast snapshots. Pulses of electrons self-disperse under space-charge broadening, so without compression, the ideal operation mode is a single electron per pulse. Here, we demonstrate femtosecond single-electron point projection microscopy (fs-ePPM) in a laser-pump fs-e-probe configuration. The electrons have an energy of only 150 eV and take tens of picoseconds to propagate to the object under study. Nonetheless, we achieve a temporal resolution with a standard deviation of 114 fs (equivalent to a full-width at half-maximum of 269 ± 40 fs) combined with a spatial resolution of 100 nm, applied to a localized region of charge at the apex of a nanoscale metal tip induced by 30 fs 800 nm laser pulses at 50 kHz. These observations demonstrate real-space imaging of reversible processes, such as tracking charge distributions, is feasible whilst maintaining femtosecond resolution. Our findings could find application as a characterization method, which, depending on geometry, could resolve tens of femtoseconds and tens of nanometres. Dynamically imaging electric and magnetic fields and charge distributions on sub-micron length scales opens new avenues of ultrafast dynamics. Furthermore, through the use of active compression, such pulses are an ideal seed for few-femtosecond to attosecond imaging applications which will access sub-optical cycle processes in nanoplasmonics. PMID:27158637

  3. A theory of phase singularities for image representation and its applications to object tracking and image matching.

    PubMed

    Qiao, Yu; Wang, Wei; Minematsu, Nobuaki; Liu, Jianzhuang; Takeda, Mitsuo; Tang, Xiaoou

    2009-10-01

    This paper studies phase singularities (PSs) for image representation. We show that PSs calculated with Laguerre-Gauss filters contain important information and provide a useful tool for image analysis. PSs are invariant to image translation and rotation. We introduce several invariant features to characterize the core structures around PSs and analyze the stability of PSs to noise addition and scale change. We also study the characteristics of PSs in a scale space, which lead to a method to select key scales along phase singularity curves. We demonstrate two applications of PSs: object tracking and image matching. In object tracking, we use the iterative closest point algorithm to determine the correspondences of PSs between two adjacent frames. The use of PSs allows us to precisely determine the motions of tracked objects. In image matching, we combine PSs and scale-invariant feature transform (SIFT) descriptor to deal with the variations between two images and examine the proposed method on a benchmark database. The results indicate that our method can find more correct matching pairs with higher repeatability rates than some well-known methods.

  4. Single photon detection imaging of Cherenkov light emitted during radiation therapy

    NASA Astrophysics Data System (ADS)

    Adamson, Philip M.; Andreozzi, Jacqueline M.; LaRochelle, Ethan; Gladstone, David J.; Pogue, Brian W.

    2018-03-01

    Cherenkov imaging during radiation therapy has been developed as a tool for dosimetry, which could have applications in patient delivery verification or in regular quality audit. The cameras used are intensified imaging sensors, either ICCD or ICMOS cameras, which allow important features of imaging, including: (1) nanosecond time gating, (2) amplification by 103-104, which together allow for imaging which has (1) real time capture at 10-30 frames per second, (2) sensitivity at the level of single photon event level, and (3) ability to suppress background light from the ambient room. However, the capability to achieve single photon imaging has not been fully analyzed to date, and as such was the focus of this study. The ability to quantitatively characterize how a single photon event appears in amplified camera imaging from the Cherenkov images was analyzed with image processing. The signal seen at normal gain levels appears to be a blur of about 90 counts in the CCD detector, after going through the chain of photocathode detection, amplification through a microchannel plate PMT, excitation onto a phosphor screen and then imaged on the CCD. The analysis of single photon events requires careful interpretation of the fixed pattern noise, statistical quantum noise distributions, and the spatial spread of each pulse through the ICCD.

  5. Objective measurements to evaluate glottal space segmentation from laryngeal images.

    PubMed

    Gutiérrez-Arriola, J M; Osma-Ruiz, V; Sáenz-Lechón, N; Godino-Llorente, J I; Fraile, R; Arias-Londoño, J D

    2012-01-01

    Objective evaluation of the results of medical image segmentation is a known problem. Applied to the task of automatically detecting the glottal area from laryngeal images, this paper proposes a new objective measurement to evaluate the quality of a segmentation algorithm by comparing with the results given by a human expert. The new figure of merit is called Area Index, and its effectiveness is compared with one of the most used figures of merit found in the literature: the Pratt Index. Results over 110 laryngeal images presented high correlations between both indexes, demonstrating that the proposed measure is comparable to the Pratt Index and it is a good indicator of the segmentation quality.

  6. Visiting two objects in the field of the ring galaxy HRG 2302

    NASA Astrophysics Data System (ADS)

    Faúndez-Abans, M.; Reshetnikov, V. P.; de Oliveira-Abans, M.; Krabbe, A. C.; da Rocha-Poppe, P. C.; Fernandes-Martin, V. A.; Amôres, E. B.; Freitas-Lemes, P.

    2015-02-01

    Aims: We investigate the nature of two galaxies that are located in the field of the ring galaxy HRG 2302. Methods: This study is based on direct BVRI imaging and long-slit spectrophotometric data in the range of 4000-9500 Å obtained with the 1.6 m telescope of the Observatório do Pico dos Dias, Brazil. The spectra were used to determine the radial velocity. Results: The primary objective of the retrieval of the photometric data was to identify the fine structures of objects H and I. In addition, we performed image processing and made a photometric analysis to obtain the integrated standard BVRI magnitudes. The contour maps show evidence of material connecting both galaxies, suggesting that they might be interacting close companions. We estimated redshifts of z = 0.0689 and z = 0.0692. The spectra of the two galaxies resemble those of an early-type galaxy. The fact that the objects have a small radial-velocity difference and the structures around object I suggest an ongoing tidal interaction between the two galaxies. Conclusions: The H-I system seems to be composed of two early-type spiral galaxies (S0/Sa). Galaxy I shows evidence of tidal perturbation: an off-centered bulge, some material extending along the NE direction, and structures that have been enhanced by image filtering procedures. There are some dwarf objects around it. Neither object shows evidence of nuclear activity. Based on observations carried out at the Observatório do Pico dos Dias (OPD), which is operated by LNA/MCTI, and public data from the LNA database.

  7. Effect diffraction on a viewed object has on improvement of object optical image quality in a turbulent medium

    NASA Astrophysics Data System (ADS)

    Banakh, Viktor A.; Sazanovich, Valentina M.; Tsvik, Ruvim S.

    1997-09-01

    The influence of diffraction on the object, coherently illuminated and viewed through a random medium from the same point, on the image quality betterment caused by the counter wave correlation is studied experimentally. The measurements were carried out with the use of setup modeling artificial convective turbulence. It is shown that in the case of spatially limited reflector with the Fresnel number of the reflector surface radius r ranging from 3 to 12 the contribution of the counter wave correlation into image intensity distribution is maximal as compared with the point objects (r objects of large size (r > U.

  8. Discovery of a Color-selected Quasar at z = 5.50.

    PubMed

    Stern; Spinrad; Eisenhardt; Bunker; Dawson; Stanford; Elston

    2000-04-20

    We present observations of RD J030117+002025, a quasar at z=5.50 discovered from deep, multicolor, ground-based observations covering 74 arcmin2. This is the most distant quasar or active galaxy currently known. The object was targeted as an R-band dropout, with RAB>26.3 (3 sigma limit in a 3&arcsec; diameter region), IAB=23.8, and zAB=23.4. The Keck/Low-Resolution Imaging Spectrometer spectrum shows broad Lyalpha/N v lambda1240 emission and sharp absorption decrements from the highly redshifted hydrogen forests. The fractional continuum depression due to the Lyalpha forest is DA=0.90. RD J030117+002025 is the least luminous high-redshift quasar known (MB approximately -22.7).

  9. Experiments on sparsity assisted phase retrieval of phase objects

    NASA Astrophysics Data System (ADS)

    Gaur, Charu; Lochab, Priyanka; Khare, Kedar

    2017-05-01

    Iterative phase retrieval algorithms such as the Gerchberg-Saxton method and the Fienup hybrid input-output method are known to suffer from the twin image stagnation problem, particularly when the solution to be recovered is complex valued and has centrosymmetric support. Recently we showed that the twin image stagnation problem can be addressed using image sparsity ideas (Gaur et al 2015 J. Opt. Soc. Am. A 32 1922). In this work we test this sparsity assisted phase retrieval method with experimental single shot Fourier transform intensity data frames corresponding to phase objects displayed on a spatial light modulator. The standard iterative phase retrieval algorithms are combined with an image sparsity based penalty in an adaptive manner. Illustrations for both binary and continuous phase objects are provided. It is observed that image sparsity constraint has an important role to play in obtaining meaningful phase recovery without encountering the well-known stagnation problems. The results are valuable for enabling single shot coherent diffraction imaging of phase objects for applications involving illumination wavelengths over a wide range of electromagnetic spectrum.

  10. How many photons are needed to reconstruct random objects in coherent X-ray diffractive imaging?

    PubMed

    Jahn, T; Wilke, R N; Chushkin, Y; Salditt, T

    2017-01-01

    This paper presents an investigation of the reconstructibility of coherent X-ray diffractive imaging diffraction patterns for a class of binary random `bitmap' objects. Combining analytical results and numerical simulations, the critical fluence per bitmap pixel is determined, for arbitrary contrast values (absorption level and phase shift), both for the optical near- and far-field. This work extends previous investigations based on information theory, enabling a comparison of the amount of information carried by single photons in different diffraction regimes. The experimental results show an order-of-magnitude agreement.

  11. Real-time detection of natural objects using AM-coded spectral matching imager

    NASA Astrophysics Data System (ADS)

    Kimachi, Akira

    2004-12-01

    This paper describes application of the amplitude-modulation (AM)-coded spectral matching imager (SMI) to real-time detection of natural objects such as human beings, animals, vegetables, or geological objects or phenomena, which are much more liable to change with time than artificial products while often exhibiting characteristic spectral functions associated with some specific activity states. The AM-SMI produces correlation between spectral functions of the object and a reference at each pixel of the correlation image sensor (CIS) in every frame, based on orthogonal amplitude modulation (AM) of each spectral channel and simultaneous demodulation of all channels on the CIS. This principle makes the SMI suitable to monitoring dynamic behavior of natural objects in real-time by looking at a particular spectral reflectance or transmittance function. A twelve-channel multispectral light source was developed with improved spatial uniformity of spectral irradiance compared to a previous one. Experimental results of spectral matching imaging of human skin and vegetable leaves are demonstrated, as well as a preliminary feasibility test of imaging a reflective object using a test color chart.

  12. Real-time detection of natural objects using AM-coded spectral matching imager

    NASA Astrophysics Data System (ADS)

    Kimachi, Akira

    2005-01-01

    This paper describes application of the amplitude-modulation (AM)-coded spectral matching imager (SMI) to real-time detection of natural objects such as human beings, animals, vegetables, or geological objects or phenomena, which are much more liable to change with time than artificial products while often exhibiting characteristic spectral functions associated with some specific activity states. The AM-SMI produces correlation between spectral functions of the object and a reference at each pixel of the correlation image sensor (CIS) in every frame, based on orthogonal amplitude modulation (AM) of each spectral channel and simultaneous demodulation of all channels on the CIS. This principle makes the SMI suitable to monitoring dynamic behavior of natural objects in real-time by looking at a particular spectral reflectance or transmittance function. A twelve-channel multispectral light source was developed with improved spatial uniformity of spectral irradiance compared to a previous one. Experimental results of spectral matching imaging of human skin and vegetable leaves are demonstrated, as well as a preliminary feasibility test of imaging a reflective object using a test color chart.

  13. Phase object retrieval through scattering medium

    NASA Astrophysics Data System (ADS)

    Zhao, Ming; Zhao, Meijing; Wu, Houde; Xu, Wenhai

    2018-05-01

    Optical imaging through a scattering medium has been an interesting and important research topic, especially in the field of biomedical imaging. However, it is still a challenging task due to strong scattering. This paper proposes to recover the phase object behind the scattering medium from one single-shot speckle intensity image using calibrated transmission matrices (TMs). We construct the forward model as a non-linear mapping, since the intensity image loses the phase information, and then a generalized phase retrieval algorithm is employed to recover the hidden object. Moreover, we show that a phase object can be reconstructed with a small portion of the speckle image captured by the camera. The simulation is performed to demonstrate our scheme and test its performance. Finally, a real experiment is set up, we measure the TMs from the scattering medium, and then use it to reconstruct the hidden object. We show that a phase object of size 32 × 32 is retrieved from 150 × 150 speckle grains, which is only 1/50 of the speckles area. We believe our proposed method can benefit the community of imaging through the scattering medium.

  14. The Large Area Radio Galaxy Evolution Spectroscopic Survey (LARGESS): survey design, data catalogue and GAMA/WiggleZ spectroscopy

    NASA Astrophysics Data System (ADS)

    Ching, John H. Y.; Sadler, Elaine M.; Croom, Scott M.; Johnston, Helen M.; Pracy, Michael B.; Couch, Warrick J.; Hopkins, A. M.; Jurek, Russell J.; Pimbblet, K. A.

    2017-01-01

    We present the Large Area Radio Galaxy Evolution Spectroscopic Survey (LARGESS), a spectroscopic catalogue of radio sources designed to include the full range of radio AGN populations out to redshift z ˜ 0.8. The catalogue covers ˜800 deg2 of sky, and provides optical identifications for 19 179 radio sources from the 1.4 GHz Faint Images of the Radio Sky at Twenty-cm (FIRST) survey down to an optical magnitude limit of Imod < 20.5 in Sloan Digital Sky Survey (SDSS) images. Both galaxies and point-like objects are included, and no colour cuts are applied. In collaboration with the WiggleZ and Galaxy And Mass Assembly (GAMA) spectroscopic survey teams, we have obtained new spectra for over 5000 objects in the LARGESS sample. Combining these new spectra with data from earlier surveys provides spectroscopic data for 12 329 radio sources in the survey area, of which 10 856 have reliable redshifts. 85 per cent of the LARGESS spectroscopic sample are radio AGN (median redshift z = 0.44), and 15 per cent are nearby star-forming galaxies (median z = 0.08). Low-excitation radio galaxies (LERGs) comprise the majority (83 per cent) of LARGESS radio AGN at z < 0.8, with 12 per cent being high-excitation radio galaxies (HERGs) and 5 per cent radio-loud QSOs. Unlike the more homogeneous LERG and QSO sub-populations, HERGs are a heterogeneous class of objects with relatively blue optical colours and a wide dispersion in mid-infrared colours. This is consistent with a picture in which most HERGs are hosted by galaxies with recent or ongoing star formation as well as a classical accretion disc.

  15. Detectors for single-molecule fluorescence imaging and spectroscopy

    PubMed Central

    MICHALET, X.; SIEGMUND, O.H.W.; VALLERGA, J.V.; JELINSKY, P.; MILLAUD, J.E.; WEISS, S.

    2010-01-01

    Single-molecule observation, characterization and manipulation techniques have recently come to the forefront of several research domains spanning chemistry, biology and physics. Due to the exquisite sensitivity, specificity, and unmasking of ensemble averaging, single-molecule fluorescence imaging and spectroscopy have become, in a short period of time, important tools in cell biology, biochemistry and biophysics. These methods led to new ways of thinking about biological processes such as viral infection, receptor diffusion and oligomerization, cellular signaling, protein-protein or protein-nucleic acid interactions, and molecular machines. Such achievements require a combination of several factors to be met, among which detector sensitivity and bandwidth are crucial. We examine here the needed performance of photodetectors used in these types of experiments, the current state of the art for different categories of detectors, and actual and future developments of single-photon counting detectors for single-molecule imaging and spectroscopy. PMID:20157633

  16. On the selection of high-z quasars using LOFAR observations

    NASA Astrophysics Data System (ADS)

    Retana-Montenegro, Edwin; Röttgering, Huub

    2018-03-01

    We present a method to identify candidate quasars which combines optical/infrared color selection with radio detections from the Low Frequency ARray (LOFAR) at 150MHz. We apply this {method} in a region of 9 square degrees located in the Boötes field, with a wealth of multi-wavelength data. Our LOFAR imaging in the central region reaches a rms noise of ˜50μJy with a resolution of 5''. This is so deep that we also routinely, `radio-quiet' quasars. We use quasar spectroscopy from the literature to calculate the completeness and efficiency of our selection method. We conduct our analysis in two redshift intervals, 1<z<2 and 2<z<3. For objects at 1.0<z<2.0, we identify 51% of the spectroscopic quasars, and 80% of our candidates are in the spectroscopic sample; while for objects at 2.0<z<3.0 these numbers are 62% and 30%, respectively. We investigate the effect of the radio spectral index distribution on our selection of candidate quasars. For this purpose, we calculate the spectral index between 1400MHz and 150MHz, by combining our LOFAR-Boötes data with 1.4GHz imaging of the Boötes field obtained with the Westerbork Synthesis Radio Telescope (WSRT), which has a rms noise of σ˜28μJy with a resolution of 13''×27''. We find that 27% of the candidate quasars are detected at 1400 MHz, and that these detected objects have a spectral index distribution with a median value of α=-0.73±0.07. Using a flux density threshold of S_{150MHz}=1.50mJy, so that all the α>-1.0 sources can be detected in the WSRT-Boötes map, we find that the spectral index distribution of the 21 quasars in the resulting sample is steeper than the general LOFAR-WSRT spectral index distribution with a median of α=-0.80±0.06. As the upcoming LOFAR wide area surveys are much deeper than the traditional 1.4GHz surveys like NVSS and FIRST, this indicates that LOFAR in combination with optical and infrared will be an excellent fishing ground to obtain large samples of quasars.

  17. Towards Single Biomolecule Imaging via Optical Nanoscale Magnetic Resonance Imaging.

    PubMed

    Boretti, Alberto; Rosa, Lorenzo; Castelletto, Stefania

    2015-09-09

    Nuclear magnetic resonance (NMR) spectroscopy is a physical marvel in which electromagnetic radiation is charged and discharged by nuclei in a magnetic field. In conventional NMR, the specific nuclei resonance frequency depends on the strength of the magnetic field and the magnetic properties of the isotope of the atoms. NMR is routinely utilized in clinical tests by converting nuclear spectroscopy in magnetic resonance imaging (MRI) and providing 3D, noninvasive biological imaging. While this technique has revolutionized biomedical science, measuring the magnetic resonance spectrum of single biomolecules is still an intangible aspiration, due to MRI resolution being limited to tens of micrometers. MRI and NMR have, however, recently greatly advanced, with many breakthroughs in nano-NMR and nano-MRI spurred by using spin sensors based on an atomic impurities in diamond. These techniques rely on magnetic dipole-dipole interactions rather than inductive detection. Here, novel nano-MRI methods based on nitrogen vacancy centers in diamond are highlighted, that provide a solution to the imaging of single biomolecules with nanoscale resolution in-vivo and in ambient conditions. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Un-collimated single-photon imaging system for high-sensitivity small animal and plant imaging.

    PubMed

    Walker, Katherine L; Judenhofer, Martin S; Cherry, Simon R; Mitchell, Gregory S

    2015-01-07

    In preclinical single-photon emission computed tomography (SPECT) system development the primary objective has been to improve spatial resolution by using novel parallel-hole or multi-pinhole collimator geometries. However, such high-resolution systems have relatively poor sensitivity (typically 0.01-0.1%). In contrast, a system that does not use collimators can achieve very high-sensitivity. Here we present a high-sensitivity un-collimated detector single-photon imaging (UCD-SPI) system for the imaging of both small animals and plants. This scanner consists of two thin, closely spaced, pixelated scintillator detectors that use NaI(Tl), CsI(Na), or BGO. The performance of the system has been characterized by measuring sensitivity, spatial resolution, linearity, detection limits, and uniformity. With (99m)Tc (140 keV) at the center of the field of view (20 mm scintillator separation), the sensitivity was measured to be 31.8% using the NaI(Tl) detectors and 40.2% with CsI(Na). The best spatial resolution (FWHM when the image formed as the geometric mean of the two detector heads, 20 mm scintillator separation) was 19.0 mm for NaI(Tl) and 11.9 mm for CsI(Na) at 140 keV, and 19.5 mm for BGO at 1116 keV, which is somewhat degraded compared to the cm-scale resolution obtained with only one detector head and a close source. The quantitative accuracy of the system's linearity is better than 2% with detection down to activity levels of 100 nCi. Two in vivo animal studies (a renal scan using (99m)Tc MAG-3 and a thyroid scan with (123)I) and one plant study (a (99m)TcO4(-) xylem transport study) highlight the unique capabilities of this UCD-SPI system. From the renal scan, we observe approximately a one thousand-fold increase in sensitivity compared to the Siemens Inveon SPECT/CT scanner. UCD-SPI is useful for many imaging tasks that do not require excellent spatial resolution, such as high-throughput screening applications, simple radiotracer uptake studies

  19. Un-collimated single-photon imaging system for high-sensitivity small animal and plant imaging

    DOE PAGES

    Walker, Katherine L.; Judenhofer, Martin S.; Cherry, Simon R.; ...

    2014-12-12

    In preclinical single-photon emission computed tomography (SPECT) system development the primary objective has been to improve spatial resolution by using novel parallel-hole or multi-pinhole collimator geometries. Furthermore, such high-resolution systems have relatively poor sensitivity (typically 0.01% to 0.1%). In contrast, a system that does not use collimators can achieve very high-sensitivity. Here we present a high-sensitivity un-collimated detector single-photon imaging (UCD-SPI) system for the imaging of both small animals and plants. This scanner consists of two thin, closely spaced, pixelated scintillator detectors that use NaI(Tl), CsI(Na), or BGO. The performance of the system has been characterized by measuring sensitivity, spatialmore » resolution, linearity, detection limits, and uniformity. With 99mTc (140 keV) at the center of the field of view (20 mm scintillator separation), the sensitivity was measured to be 31.8% using the NaI(Tl) detectors and 40.2% with CsI(Na). The best spatial resolution (FWHM when the image formed as the geometric mean of the two detector heads, 20 mm scintillator separation) was 19.0 mm for NaI(Tl) and 11.9 mm for CsI(Na) at 140 keV, and 19.5 mm for BGO at 1116 keV, which is somewhat degraded compared to the cm-scale resolution obtained with only one detector head and a close source. The quantitative accuracy of the system’s linearity is better than 2% with detection down to activity levels of 100 nCi. Two in vivo animal studies (a renal scan using 99mTc MAG-3 and a thyroid scan with 123I) and one plant study (a 99mTcO 4- xylem transport study) highlight the unique capabilities of this UCD-SPI system. From the renal scan, we observe approximately a one thousand-fold increase in sensitivity compared to the Siemens Inveon SPECT/CT scanner. In conclusion, UCD-SPI is useful for many imaging tasks that do not require excellent spatial resolution, such as high-throughput screening applications, simple radiotracer uptake

  20. Un-collimated single-photon imaging system for high-sensitivity small animal and plant imaging

    NASA Astrophysics Data System (ADS)

    Walker, Katherine L.; Judenhofer, Martin S.; Cherry, Simon R.; Mitchell, Gregory S.

    2015-01-01

    In preclinical single-photon emission computed tomography (SPECT) system development the primary objective has been to improve spatial resolution by using novel parallel-hole or multi-pinhole collimator geometries. However, such high-resolution systems have relatively poor sensitivity (typically 0.01-0.1%). In contrast, a system that does not use collimators can achieve very high-sensitivity. Here we present a high-sensitivity un-collimated detector single-photon imaging (UCD-SPI) system for the imaging of both small animals and plants. This scanner consists of two thin, closely spaced, pixelated scintillator detectors that use NaI(Tl), CsI(Na), or BGO. The performance of the system has been characterized by measuring sensitivity, spatial resolution, linearity, detection limits, and uniformity. With 99mTc (140 keV) at the center of the field of view (20 mm scintillator separation), the sensitivity was measured to be 31.8% using the NaI(Tl) detectors and 40.2% with CsI(Na). The best spatial resolution (FWHM when the image formed as the geometric mean of the two detector heads, 20 mm scintillator separation) was 19.0 mm for NaI(Tl) and 11.9 mm for CsI(Na) at 140 keV, and 19.5 mm for BGO at 1116 keV, which is somewhat degraded compared to the cm-scale resolution obtained with only one detector head and a close source. The quantitative accuracy of the system’s linearity is better than 2% with detection down to activity levels of 100 nCi. Two in vivo animal studies (a renal scan using 99mTc MAG-3 and a thyroid scan with 123I) and one plant study (a 99mTcO4- xylem transport study) highlight the unique capabilities of this UCD-SPI system. From the renal scan, we observe approximately a one thousand-fold increase in sensitivity compared to the Siemens Inveon SPECT/CT scanner. UCD-SPI is useful for many imaging tasks that do not require excellent spatial resolution, such as high-throughput screening applications, simple radiotracer uptake studies in tumor

  1. Multi-energy method of digital radiography for imaging of biological objects

    NASA Astrophysics Data System (ADS)

    Ryzhikov, V. D.; Naydenov, S. V.; Opolonin, O. D.; Volkov, V. G.; Smith, C. F.

    2016-03-01

    This work has been dedicated to the search for a new possibility to use multi-energy digital radiography (MER) for medical applications. Our work has included both theoretical and experimental investigations of 2-energy (2E) and 3- energy (3D) radiography for imaging the structure of biological objects. Using special simulation methods and digital analysis based on the X-ray interaction energy dependence for each element of importance to medical applications in the X-ray range of energy up to 150 keV, we have implemented a quasi-linear approximation for the energy dependence of the X-ray linear mass absorption coefficient μm (E) that permits us to determine the intrinsic structure of the biological objects. Our measurements utilize multiple X-ray tube voltages (50, 100, and 150 kV) with Al and Cu filters of different thicknesses to achieve 3-energy X-ray examination of objects. By doing so, we are able to achieve significantly improved imaging quality of the structure of the subject biological objects. To reconstruct and visualize the final images, we use both two-dimensional (2D) and three-dimensional (3D) palettes of identification. The result is a 2E and/or 3E representation of the object with color coding of each pixel according to the data outputs. Following the experimental measurements and post-processing, we produce a 3D image of the biological object - in the case of our trials, fragments or parts of chicken and turkey.

  2. Can state-of-the-art HVS-based objective image quality criteria be used for image reconstruction techniques based on ROI analysis?

    NASA Astrophysics Data System (ADS)

    Dostal, P.; Krasula, L.; Klima, M.

    2012-06-01

    Various image processing techniques in multimedia technology are optimized using visual attention feature of the human visual system. Spatial non-uniformity causes that different locations in an image are of different importance in terms of perception of the image. In other words, the perceived image quality depends mainly on the quality of important locations known as regions of interest. The performance of such techniques is measured by subjective evaluation or objective image quality criteria. Many state-of-the-art objective metrics are based on HVS properties; SSIM, MS-SSIM based on image structural information, VIF based on the information that human brain can ideally gain from the reference image or FSIM utilizing the low-level features to assign the different importance to each location in the image. But still none of these objective metrics utilize the analysis of regions of interest. We solve the question if these objective metrics can be used for effective evaluation of images reconstructed by processing techniques based on ROI analysis utilizing high-level features. In this paper authors show that the state-of-the-art objective metrics do not correlate well with subjective evaluation while the demosaicing based on ROI analysis is used for reconstruction. The ROI were computed from "ground truth" visual attention data. The algorithm combining two known demosaicing techniques on the basis of ROI location is proposed to reconstruct the ROI in fine quality while the rest of image is reconstructed with low quality. The color image reconstructed by this ROI approach was compared with selected demosaicing techniques by objective criteria and subjective testing. The qualitative comparison of the objective and subjective results indicates that the state-of-the-art objective metrics are still not suitable for evaluation image processing techniques based on ROI analysis and new criteria is demanded.

  3. Extracting 3D Parametric Curves from 2D Images of Helical Objects.

    PubMed

    Willcocks, Chris G; Jackson, Philip T G; Nelson, Carl J; Obara, Boguslaw

    2017-09-01

    Helical objects occur in medicine, biology, cosmetics, nanotechnology, and engineering. Extracting a 3D parametric curve from a 2D image of a helical object has many practical applications, in particular being able to extract metrics such as tortuosity, frequency, and pitch. We present a method that is able to straighten the image object and derive a robust 3D helical curve from peaks in the object boundary. The algorithm has a small number of stable parameters that require little tuning, and the curve is validated against both synthetic and real-world data. The results show that the extracted 3D curve comes within close Hausdorff distance to the ground truth, and has near identical tortuosity for helical objects with a circular profile. Parameter insensitivity and robustness against high levels of image noise are demonstrated thoroughly and quantitatively.

  4. Measurement of Radiation Symmetry in Z-Pinch Driven Hohlraums

    NASA Astrophysics Data System (ADS)

    Hanson, David L.

    2001-10-01

    The z-pinch driven hohlraum (ZPDH) is a promising approach to high yield inertial confinement fusion currently being characterized in experiments on the Sandia Z accelerator [1]. In this concept [2], x rays are produced by an axial z-pinch in a primary hohlraum at each end of a secondary hohlraum. A fusion capsule in the secondary is imploded by a symmetric x-ray flux distribution, effectively smoothed by wall reemission during transport to the capsule position. Capsule radiation symmetry, a critical issue in the design of such a system, is influenced by hohlraum geometry, wall motion and time-dependent albedo, as well as power balance and pinch timing between the two z-pinch x-ray sources. In initial symmetry studies on Z, we used solid low density burnthrough spheres to diagnose highly asymmetric, single-sided-drive hohlraum geometries. We then applied this technique to the more symmetric double z-pinch geometry [3]. As a result of design improvements, radiation flux symmetry in Z double-pinch wire array experiments now exceeds the measurement sensitivity of this self-backlit foam ball symmetry diagnostic (15% max-min flux asymmetry). To diagnose radiation symmetry at the 2 - 5% level attainable with our present ZPDH designs, we are using high-energy x rays produced by the recently-completed Z-Beamlet laser backlighter for point-projection imaging of thin-wall implosion and symmetry capsules. We will present the results of polar flux symmetry measuremets on Z for several ZPDH capsule geometries together with radiosity and radiation-hydrodynamics simulations for comparison. [1] M. E. Cuneo et al., Phys. Plasmas 8,2257(2001); [2] J. H. Hammer et al., Phys. Plasmas 6,2129(1999); [3] D. L. Hanson et al., Bull. Am. Phys. Soc. 45,360(2000).

  5. Critical object recognition in millimeter-wave images with robustness to rotation and scale.

    PubMed

    Mohammadzade, Hoda; Ghojogh, Benyamin; Faezi, Sina; Shabany, Mahdi

    2017-06-01

    Locating critical objects is crucial in various security applications and industries. For example, in security applications, such as in airports, these objects might be hidden or covered under shields or secret sheaths. Millimeter-wave images can be utilized to discover and recognize the critical objects out of the hidden cases without any health risk due to their non-ionizing features. However, millimeter-wave images usually have waves in and around the detected objects, making object recognition difficult. Thus, regular image processing and classification methods cannot be used for these images and additional pre-processings and classification methods should be introduced. This paper proposes a novel pre-processing method for canceling rotation and scale using principal component analysis. In addition, a two-layer classification method is introduced and utilized for recognition. Moreover, a large dataset of millimeter-wave images is collected and created for experiments. Experimental results show that a typical classification method such as support vector machines can recognize 45.5% of a type of critical objects at 34.2% false alarm rate (FAR), which is a drastically poor recognition. The same method within the proposed recognition framework achieves 92.9% recognition rate at 0.43% FAR, which indicates a highly significant improvement. The significant contribution of this work is to introduce a new method for analyzing millimeter-wave images based on machine vision and learning approaches, which is not yet widely noted in the field of millimeter-wave image analysis.

  6. Pressure-induced Polarization Reversal in Z-type Hexaferrite Single Crystal

    NASA Astrophysics Data System (ADS)

    Jeon, Byung-Gu; Chun, Sae Hwan; Kim, Kee Hoon

    2012-02-01

    Multiferroic materials with a gigantic magnetoelectric (ME) coupling at room temperature have been searched for applications to novel devices. Recently, large direct and converse ME effects were realized at room temperature in the so-called Z-type hexaferrite (Ba,Sr)3Co2Fe24O41 single crystals [1,2]. To obtain a new control parameter for realizing a sensitive ME tuning, we studied ME properties of the crystals under uniaxial pressure. Upon applying a tiny uniaxial pressure of about 0.6 GPa, magnetic field-driven electric polarization reversal and anomaly in a M-H loop start to appear at 10 K and gradually disappear at higher temperature above 130 K. By comparing those results with longitudinal magnetostriction at ambient pressure, we propose the pressure-dependent variations of transverse conical spin configuration as well as its domain structure under small magnetic field bias, and point out the possibility of having two different physical origins of the ME coupling in this system. [1] Y. Kitagawa et al., Nat. Mater. 9, 797 (2010) [2] S. H. Chun et al., submitted.

  7. Neutron Imaging Developments at LANSCE

    NASA Astrophysics Data System (ADS)

    Nelson, Ron; Hunter, James; Schirato, Richard; Vogel, Sven; Swift, Alicia; Ickes, Tim; Ward, Bill; Losko, Adrian; Tremsin, Anton

    2015-10-01

    Neutron imaging is complementary to x-ray imaging because of its sensitivity to light elements and greater penetration of high-Z materials. Energy-resolved neutron imaging can provide contrast enhancements for elements and isotopes due to the variations with energy in scattering cross sections due to nuclear resonances. These cross section differences exist due to compound nuclear resonances that are characteristic of each element and isotope, as well as broader resonances at higher energies. In addition, multi-probe imaging, such as combined photon and neutron imaging, is a powerful tool for discerning properties and features in materials that cannot be observed with a single probe. Recently, we have demonstrated neutron imaging, both radiography and computed tomography, using the moderated (Lujan Center) and high-energy (WNR facility) neutron sources at LANSCE. Flat panel x-ray detectors with suitable scintillator-converter screens provide good sensitivity for both low and high neutron energies. Micro-Channel-Plate detectors and iCCD scintillator camera systems that provide the fast time gating needed for energy-resolved imaging have been demonstrated as well. Examples of recent work will be shown including fluid flow in plants and imaging through dense thick objects. This work is funded by the US Department of Energy, National Nuclear Security Administration, and performed by Los Alamos National Security LLC under Contract DE-AC52-06NA25396.

  8. Algorithms for detection of objects in image sequences captured from an airborne imaging system

    NASA Technical Reports Server (NTRS)

    Kasturi, Rangachar; Camps, Octavia; Tang, Yuan-Liang; Devadiga, Sadashiva; Gandhi, Tarak

    1995-01-01

    This research was initiated as a part of the effort at the NASA Ames Research Center to design a computer vision based system that can enhance the safety of navigation by aiding the pilots in detecting various obstacles on the runway during critical section of the flight such as a landing maneuver. The primary goal is the development of algorithms for detection of moving objects from a sequence of images obtained from an on-board video camera. Image regions corresponding to the independently moving objects are segmented from the background by applying constraint filtering on the optical flow computed from the initial few frames of the sequence. These detected regions are tracked over subsequent frames using a model based tracking algorithm. Position and velocity of the moving objects in the world coordinate is estimated using an extended Kalman filter. The algorithms are tested using the NASA line image sequence with six static trucks and a simulated moving truck and experimental results are described. Various limitations of the currently implemented version of the above algorithm are identified and possible solutions to build a practical working system are investigated.

  9. Image analysis driven single-cell analytics for systems microbiology.

    PubMed

    Balomenos, Athanasios D; Tsakanikas, Panagiotis; Aspridou, Zafiro; Tampakaki, Anastasia P; Koutsoumanis, Konstantinos P; Manolakos, Elias S

    2017-04-04

    Time-lapse microscopy is an essential tool for capturing and correlating bacterial morphology and gene expression dynamics at single-cell resolution. However state-of-the-art computational methods are limited in terms of the complexity of cell movies that they can analyze and lack of automation. The proposed Bacterial image analysis driven Single Cell Analytics (BaSCA) computational pipeline addresses these limitations thus enabling high throughput systems microbiology. BaSCA can segment and track multiple bacterial colonies and single-cells, as they grow and divide over time (cell segmentation and lineage tree construction) to give rise to dense communities with thousands of interacting cells in the field of view. It combines advanced image processing and machine learning methods to deliver very accurate bacterial cell segmentation and tracking (F-measure over 95%) even when processing images of imperfect quality with several overcrowded colonies in the field of view. In addition, BaSCA extracts on the fly a plethora of single-cell properties, which get organized into a database summarizing the analysis of the cell movie. We present alternative ways to analyze and visually explore the spatiotemporal evolution of single-cell properties in order to understand trends and epigenetic effects across cell generations. The robustness of BaSCA is demonstrated across different imaging modalities and microscopy types. BaSCA can be used to analyze accurately and efficiently cell movies both at a high resolution (single-cell level) and at a large scale (communities with many dense colonies) as needed to shed light on e.g. how bacterial community effects and epigenetic information transfer play a role on important phenomena for human health, such as biofilm formation, persisters' emergence etc. Moreover, it enables studying the role of single-cell stochasticity without losing sight of community effects that may drive it.

  10. Star formation rate and extinction in faint z ∼ 4 Lyman break galaxies

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    To, Chun-Hao; Wang, Wei-Hao; Owen, Frazer N.

    We present a statistical detection of 1.5 GHz radio continuum emission from a sample of faint z ∼ 4 Lyman break galaxies (LBGs). To constrain their extinction and intrinsic star formation rate (SFR), we combine the latest ultradeep Very Large Array 1.5 GHz radio image and the Hubble Space Telescope Advanced Camera for Surveys (ACS) optical images in the GOODS-N. We select a large sample of 1771 z ∼ 4 LBGs from the ACS catalog using B {sub F435W}-dropout color criteria. Our LBG samples have I {sub F775W} ∼ 25-28 (AB), ∼0-3 mag fainter than M{sub UV}{sup ⋆} at zmore » ∼ 4. In our stacked radio images, we find the LBGs to be point-like under our 2'' angular resolution. We measure their mean 1.5 GHz flux by stacking the measurements on the individual objects. We achieve a statistical detection of S {sub 1.5} {sub GHz} = 0.210 ± 0.075 μJy at ∼3σ for the first time on such a faint LBG population at z ∼ 4. The measurement takes into account the effects of source size and blending of multiple objects. The detection is visually confirmed by stacking the radio images of the LBGs, and the uncertainty is quantified with Monte Carlo simulations on the radio image. The stacked radio flux corresponds to an obscured SFR of 16.0 ± 5.7 M {sub ☉} yr{sup –1}, and implies a rest-frame UV extinction correction factor of 3.8. This extinction correction is in excellent agreement with that derived from the observed UV continuum spectral slope, using the local calibration of Meurer et al. This result supports the use of the local calibration on high-redshift LBGs to derive the extinction correction and SFR, and also disfavors a steep reddening curve such as that of the Small Magellanic Cloud.« less

  11. High-performance imaging of stem cells using single-photon emissions

    NASA Astrophysics Data System (ADS)

    Wagenaar, Douglas J.; Moats, Rex A.; Hartsough, Neal E.; Meier, Dirk; Hugg, James W.; Yang, Tang; Gazit, Dan; Pelled, Gadi; Patt, Bradley E.

    2011-10-01

    Radiolabeled cells have been imaged for decades in the field of autoradiography. Recent advances in detector and microelectronics technologies have enabled the new field of "digital autoradiography" which remains limited to ex vivo specimens of thin tissue slices. The 3D field-of-view (FOV) of single cell imaging can be extended to millimeters if the low energy (10-30 keV) photon emissions of radionuclides are used for single-photon nuclear imaging. This new microscope uses a coded aperture foil made of highly attenuating elements such as gold or platinum to form the image as a kind of "lens". The detectors used for single-photon emission microscopy are typically silicon detectors with a pixel pitch less than 60 μm. The goal of this work is to image radiolabeled mesenchymal stem cells in vivo in an animal model of tendon repair processes. Single-photon nuclear imaging is an attractive modality for translational medicine since the labeled cells can be imaged simultaneously with the reparative processes by using the dual-isotope imaging technique. The details our microscope's two-layer gold aperture and the operation of the energy-dispersive, pixellated silicon detector are presented along with the first demonstration of energy discrimination with a 57Co source. Cell labeling techniques have been augmented by genetic engineering with the sodium-iodide symporter, a type of reporter gene imaging method that enables in vivo uptake of free 99mTc or an iodine isotope at a time point days or weeks after the insertion of the genetically modified stem cells into the animal model. This microscopy work in animal research may expand to the imaging of reporter-enabled stem cells simultaneously with the expected biological repair process in human clinical trials of stem cell therapies.

  12. Combined self-learning based single-image super-resolution and dual-tree complex wavelet transform denoising for medical images

    NASA Astrophysics Data System (ADS)

    Yang, Guang; Ye, Xujiong; Slabaugh, Greg; Keegan, Jennifer; Mohiaddin, Raad; Firmin, David

    2016-03-01

    In this paper, we propose a novel self-learning based single-image super-resolution (SR) method, which is coupled with dual-tree complex wavelet transform (DTCWT) based denoising to better recover high-resolution (HR) medical images. Unlike previous methods, this self-learning based SR approach enables us to reconstruct HR medical images from a single low-resolution (LR) image without extra training on HR image datasets in advance. The relationships between the given image and its scaled down versions are modeled using support vector regression with sparse coding and dictionary learning, without explicitly assuming reoccurrence or self-similarity across image scales. In addition, we perform DTCWT based denoising to initialize the HR images at each scale instead of simple bicubic interpolation. We evaluate our method on a variety of medical images. Both quantitative and qualitative results show that the proposed approach outperforms bicubic interpolation and state-of-the-art single-image SR methods while effectively removing noise.

  13. Automatic image database generation from CAD for 3D object recognition

    NASA Astrophysics Data System (ADS)

    Sardana, Harish K.; Daemi, Mohammad F.; Ibrahim, Mohammad K.

    1993-06-01

    The development and evaluation of Multiple-View 3-D object recognition systems is based on a large set of model images. Due to the various advantages of using CAD, it is becoming more and more practical to use existing CAD data in computer vision systems. Current PC- level CAD systems are capable of providing physical image modelling and rendering involving positional variations in cameras, light sources etc. We have formulated a modular scheme for automatic generation of various aspects (views) of the objects in a model based 3-D object recognition system. These views are generated at desired orientations on the unit Gaussian sphere. With a suitable network file sharing system (NFS), the images can directly be stored on a database located on a file server. This paper presents the image modelling solutions using CAD in relation to multiple-view approach. Our modular scheme for data conversion and automatic image database storage for such a system is discussed. We have used this approach in 3-D polyhedron recognition. An overview of the results, advantages and limitations of using CAD data and conclusions using such as scheme are also presented.

  14. Coherent and incoherent imaging through scattering media (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Edrei, Eitan

    2017-02-01

    The shower-curtain effect is a familiar phenomenon, routinely observed in our everyday life: an object placed behind a scattering layer appears blurred but if the object is attached to the scattering layer it can be clearly resolved. The optical system we developed takes advantage of the shower-curtain effect properties and generalizes them to achieve high-resolution imaging of objects placed at a nearly arbitrary distance behind the scattering medium. The imaging procedure is based on retrieving the object Fourier transform from the turbid medium (used as the shower-curtain) through a correlography technique based on speckle illumination. Illuminating the object with a speckle pattern rather than a coherent beam, we show that the correlography principles can be effectively applied in the near field. While the far-field condition is usually known as z<(2D^2)⁄λ (D, size of the object; λ wavelength); by tuning the spatial coherence of the illumination beam, as one can do with speckle illumination, the "far-field" condition can be written as z<(2DRc)⁄λ where Rc is the correlation radius of the speckle pattern. Using our method we present high-resolution imaging of objects hidden behind millimeter-thick tissue or dense lens cataracts, and demonstrate our imaging technique to be insensitive to rapid medium movements (<5 m/s) beyond any biologically relevant motion. Furthermore, we show this method can be extended to several contrast mechanisms and imaging configurations.

  15. A Statistical Study of Multiply Imaged Systems in the Lensing Cluster Abell 68

    NASA Astrophysics Data System (ADS)

    Richard, Johan; Kneib, Jean-Paul; Jullo, Eric; Covone, Giovanni; Limousin, Marceau; Ellis, Richard; Stark, Daniel; Bundy, Kevin; Czoske, Oliver; Ebeling, Harald; Soucail, Geneviève

    2007-06-01

    We have carried out an extensive spectroscopic survey with the Keck and VLT telescopes, targeting lensed galaxies in the background of the massive cluster Abell 68. Spectroscopic measurements are obtained for 26 lensed images, including a distant galaxy at z=5.4. Redshifts have been determined for 5 out of 7 multiple-image systems. Through a careful modeling of the mass distribution in the strongly lensed regime, we derive a mass estimate of 5.3×1014 Msolar within 500 kpc. Our mass model is then used to constrain the redshift distribution of the remaining multiply imaged and singly imaged sources. This enables us to examine the physical properties for a subsample of 7 Lyα emitters at 1.7<~z<~5.5, whose unlensed luminosities of ~=1041 ergs s-1 are fainter than similar objects found in blank fields. Of particular interest is an extended Lyα emission region surrounding a highly magnified source at z=2.6, detected in VIMOS integral field spectroscopy data. The physical scale of the most distant lensed source at z=5.4 is very small (<300 pc), similar to the lensed z~5.6 emitter reported by Ellis et al. in Abell 2218. New photometric data available for Abell 2218 allow for a direct comparison between these two unique objects. Our survey illustrates the practicality of using lensing clusters to probe the faint end of the z~2-5 Lyα luminosity function in a manner that is complementary to blank-field narrowband surveys. Data presented herein were obtained at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California and the National Aeronautics and Space Administration. The Observatory was made possible by the generous financial support of the W. M. Keck Foundation. Also based on observations collected at the Very Large Telescope (Antu/UT1 and Melipal/UT3), European Southern Observatory, Paranal, Chile (ESO programs 070.A-0643 and 073.A-0774), the NASA/ESA Hubble Space Telescope

  16. A simple derivation and analysis of a helical cone beam tomographic algorithm for long object imaging via a novel definition of region of interest

    NASA Astrophysics Data System (ADS)

    Hu, Jicun; Tam, Kwok; Johnson, Roger H.

    2004-01-01

    We derive and analyse a simple algorithm first proposed by Kudo et al (2001 Proc. 2001 Meeting on Fully 3D Image Reconstruction in Radiology and Nuclear Medicine (Pacific Grove, CA) pp 7-10) for long object imaging from truncated helical cone beam data via a novel definition of region of interest (ROI). Our approach is based on the theory of short object imaging by Kudo et al (1998 Phys. Med. Biol. 43 2885-909). One of the key findings in their work is that filtering of the truncated projection can be divided into two parts: one, finite in the axial direction, results from ramp filtering the data within the Tam window. The other, infinite in the z direction, results from unbounded filtering of ray sums over PI lines only. We show that for an ROI defined by PI lines emanating from the initial and final source positions on a helical segment, the boundary data which would otherwise contaminate the reconstruction of the ROI can be completely excluded. This novel definition of the ROI leads to a simple algorithm for long object imaging. The overscan of the algorithm is analytically calculated and it is the same as that of the zero boundary method. The reconstructed ROI can be divided into two regions: one is minimally contaminated by the portion outside the ROI, while the other is reconstructed free of contamination. We validate the algorithm with a 3D Shepp-Logan phantom and a disc phantom.

  17. Digital holographic image fusion for a larger size object using compressive sensing

    NASA Astrophysics Data System (ADS)

    Tian, Qiuhong; Yan, Liping; Chen, Benyong; Yao, Jiabao; Zhang, Shihua

    2017-05-01

    Digital holographic imaging fusion for a larger size object using compressive sensing is proposed. In this method, the high frequency component of the digital hologram under discrete wavelet transform is represented sparsely by using compressive sensing so that the data redundancy of digital holographic recording can be resolved validly, the low frequency component is retained totally to ensure the image quality, and multiple reconstructed images with different clear parts corresponding to a laser spot size are fused to realize the high quality reconstructed image of a larger size object. In addition, a filter combing high-pass and low-pass filters is designed to remove the zero-order term from a digital hologram effectively. The digital holographic experimental setup based on off-axis Fresnel digital holography was constructed. The feasible and comparative experiments were carried out. The fused image was evaluated by using the Tamura texture features. The experimental results demonstrated that the proposed method can improve the processing efficiency and visual characteristics of the fused image and enlarge the size of the measured object effectively.

  18. Monocular correspondence detection for symmetrical objects by template matching

    NASA Astrophysics Data System (ADS)

    Vilmar, G.; Besslich, Philipp W., Jr.

    1990-09-01

    We describe a possibility to reconstruct 3-D information from a single view of an 3-D bilateral symmetric object. The symmetry assumption allows us to obtain a " second view" from a different viewpoint by a simple reflection of the monocular image. Therefore we have to solve the correspondence problem in a special case where known feature-based or area-based binocular approaches fail. In principle our approach is based on a frequency domain template matching of the features on the epipolar lines. During a training period our system " learns" the assignment of correspondence models to image features. The object shape is interpolated when no template matches to the image features. This fact is an important advantage of this methodology because no " real world" image holds the symmetry assumption perfectly. To simplify the training process we used single views on human faces (e. g. passport photos) but our system is trainable on any other kind of objects.

  19. Whole-surface round object imaging method using line-scan hyperspectral imaging system

    USDA-ARS?s Scientific Manuscript database

    To achieve comprehensive online quality and safety inspection of fruits, whole-surface sample presentation and imaging regimes must be considered. Specifically, a round object sample presentation method is under development to achieve effective whole-surface sample evaluation based on the use of a s...

  20. Geographic Object-Based Image Analysis - Towards a new paradigm.

    PubMed

    Blaschke, Thomas; Hay, Geoffrey J; Kelly, Maggi; Lang, Stefan; Hofmann, Peter; Addink, Elisabeth; Queiroz Feitosa, Raul; van der Meer, Freek; van der Werff, Harald; van Coillie, Frieke; Tiede, Dirk

    2014-01-01

    The amount of scientific literature on (Geographic) Object-based Image Analysis - GEOBIA has been and still is sharply increasing. These approaches to analysing imagery have antecedents in earlier research on image segmentation and use GIS-like spatial analysis within classification and feature extraction approaches. This article investigates these development and its implications and asks whether or not this is a new paradigm in remote sensing and Geographic Information Science (GIScience). We first discuss several limitations of prevailing per-pixel methods when applied to high resolution images. Then we explore the paradigm concept developed by Kuhn (1962) and discuss whether GEOBIA can be regarded as a paradigm according to this definition. We crystallize core concepts of GEOBIA, including the role of objects, of ontologies and the multiplicity of scales and we discuss how these conceptual developments support important methods in remote sensing such as change detection and accuracy assessment. The ramifications of the different theoretical foundations between the ' per-pixel paradigm ' and GEOBIA are analysed, as are some of the challenges along this path from pixels, to objects, to geo-intelligence. Based on several paradigm indications as defined by Kuhn and based on an analysis of peer-reviewed scientific literature we conclude that GEOBIA is a new and evolving paradigm.

  1. Recent technology and usage of plastic lenses in image taking objectives

    NASA Astrophysics Data System (ADS)

    Yamaguchi, Susumu; Sato, Hiroshi; Mori, Nobuyoshi; Kiriki, Toshihiko

    2005-09-01

    Recently, plastic lenses produced by injection molding are widely used in image taking objectives for digital cameras, camcorders, and mobile phone cameras, because of their suitability for volume production and ease of obtaining an advantage of aspherical surfaces. For digital camera and camcorder objectives, it is desirable that there is no image point variation with the temperature change in spite of employing several plastic lenses. At the same time, due to the shrinking pixel size of solid-state image sensor, there is now a requirement to assemble lenses with high accuracy. In order to satisfy these requirements, we have developed 16 times compact zoom objective for camcorder and 3 times class folded zoom objectives for digital camera, incorporating cemented plastic doublet consisting of a positive lens and a negative lens. Over the last few years, production volumes of camera-equipped mobile phones have increased substantially. Therefore, for mobile phone cameras, the consideration of productivity is more important than ever. For this application, we have developed a 1.3-mega pixels compact camera module with macro function utilizing the advantage of a plastic lens that can be given mechanically functional shape to outer flange part. Its objective consists of three plastic lenses and all critical dimensions related to optical performance can be determined by high precise optical elements. Therefore this camera module is manufactured without optical adjustment in automatic assembling line, and achieves both high productivity and high performance. Reported here are the constructions and the technical topics of image taking objectives described above.

  2. Toward objective image quality metrics: the AIC Eval Program of the JPEG

    NASA Astrophysics Data System (ADS)

    Richter, Thomas; Larabi, Chaker

    2008-08-01

    Objective quality assessment of lossy image compression codecs is an important part of the recent call of the JPEG for Advanced Image Coding. The target of the AIC ad-hoc group is twofold: First, to receive state-of-the-art still image codecs and to propose suitable technology for standardization; and second, to study objective image quality metrics to evaluate the performance of such codes. Even tthough the performance of an objective metric is defined by how well it predicts the outcome of a subjective assessment, one can also study the usefulness of a metric in a non-traditional way indirectly, namely by measuring the subjective quality improvement of a codec that has been optimized for a specific objective metric. This approach shall be demonstrated here on the recently proposed HDPhoto format14 introduced by Microsoft and a SSIM-tuned17 version of it by one of the authors. We compare these two implementations with JPEG1 in two variations and a visual and PSNR optimal JPEG200013 implementation. To this end, we use subjective and objective tests based on the multiscale SSIM and a new DCT based metric.

  3. Preliminary experimental results of tungsten wire-array Z-pinches on primary test stand

    NASA Astrophysics Data System (ADS)

    Huang, Xian-Bin; Zhou, Shao-Tong; Dan, Jia-Kun; Ren, Xiao-Dong; Wang, Kun-Lun; Zhang, Si-Qun; Li, Jing; Xu, Qiang; Cai, Hong-Chun; Duan, Shu-Chao; Ouyang, Kai; Chen, Guang-Hua; Ji, Ce; Wei, Bing; Feng, Shu-Ping; Wang, Meng; Xie, Wei-Ping; Deng, Jian-Jun; Zhou, Xiu-Wen; Yang, Yi

    2015-07-01

    The Primary Test Stand (PTS) developed at the China Academy of Engineering Physics is a 20 TW pulsed power driver, which can deliver a ˜10 MA, 70 ns rise-time (10%-90%) current to a short-circuit load and has important applications in Z-pinch driven inertial confinement fusion and high energy density physics. Preliminary results of tungsten wire-array Z-pinch experiments on PTS are presented. The load geometries investigated include 15-mm-tall cylindrical single and nested arrays with diameter ranging from 13 mm to 30 mm, consisting of 132-300 tungsten wires with 5-10 μm in diameter. Multiple diagnostics were fielded to characterize the x-ray radiation from wire-array Z pinches. The x-ray peak power (˜50 TW) and total radiated energy (˜500 kJ) were obtained from a single 20-mm-diam array with 80-ns stagnation time. The highest x-ray peak power up to 80 TW with 2.4 ns FWHM was achieved by using a nested array with 20-mm outer diameter, and the total x-ray energy from the nested array is comparable to that of single array. Implosion velocity estimated from the time-resolved image measurement exceeds 30 cm/μs. The detailed experimental results and other findings are presented and discussed.

  4. A foreground object features-based stereoscopic image visual comfort assessment model

    NASA Astrophysics Data System (ADS)

    Jin, Xin; Jiang, G.; Ying, H.; Yu, M.; Ding, S.; Peng, Z.; Shao, F.

    2014-11-01

    Since stereoscopic images provide observers with both realistic and discomfort viewing experience, it is necessary to investigate the determinants of visual discomfort. By considering that foreground object draws most attention when human observing stereoscopic images. This paper proposes a new foreground object based visual comfort assessment (VCA) metric. In the first place, a suitable segmentation method is applied to disparity map and then the foreground object is ascertained as the one having the biggest average disparity. In the second place, three visual features being average disparity, average width and spatial complexity of foreground object are computed from the perspective of visual attention. Nevertheless, object's width and complexity do not consistently influence the perception of visual comfort in comparison with disparity. In accordance with this psychological phenomenon, we divide the whole images into four categories on the basis of different disparity and width, and exert four different models to more precisely predict its visual comfort in the third place. Experimental results show that the proposed VCA metric outperformance other existing metrics and can achieve a high consistency between objective and subjective visual comfort scores. The Pearson Linear Correlation Coefficient (PLCC) and Spearman Rank Order Correlation Coefficient (SROCC) are over 0.84 and 0.82, respectively.

  5. Tracking single membrane targets of human autoantibodies using single nanoparticle imaging.

    PubMed

    Jézéquel, Julie; Dupuis, Julien P; Maingret, François; Groc, Laurent

    2018-04-21

    Over the past decade, an increasing number of neurological and neuropsychiatric diseases have been associated with the expression of autoantibodies directed against neuronal targets, including neurotransmitter receptors. Although cell-based assays are routinely used in clinics to detect the presence of immunoglobulins, such tests often provide heterogeneous outcomes due to their limited sensitivity, especially at low titers. Thus, there is an urging need for new methods allowing the detection of autoantibodies in seropositive patients that cannot always be clinically distinguished from seronegative ones. Here we make a case for single nanoparticle imaging approaches as a highly sensitive antibody detection assay. Through high-affinity interactions between functionalized nanoparticles and autoantibodies that recognize extracellular domains of membrane neuronal targets, single nanoparticle imaging allows a live surface staining of transmembrane proteins and gives access to their surface dynamics. We show here that this method is well-suited to detect low titers of purified immunoglobulin G (IgG) from first-episode psychotic patients and demonstrate that these IgG target glutamatergic N-Methyl-d-Aspartate receptors (NMDAR) in live hippocampal neurons. The molecular behaviors of targeted membrane receptors were indistinguishable from those of endogenous GluN1 NMDAR subunit and were virtually independent of the IgG concentration present in the sample contrary to classical cell-based assays. Single nanoparticle imaging emerges as a real-time sensitive method to detect IgG directed against neuronal surface proteins, which could be used as an additional step to rule out ambiguous seropositivity diagnoses. Copyright © 2018 Elsevier B.V. All rights reserved.

  6. Fine Resolution Imaging of Space Objects.

    DTIC Science & Technology

    1982-02-01

    Figur einschliesst," J. reine agnew. Math. 123, 241 (1901). 5. J. Christou, "Imaging of Star Clusters from Speckle Inter- ferometry," Opt. Commun...Also, at any point x, by definition g.+,(x) is the near- est value to gMx ) that satisfies the object-domain constraints. Therefore, at all...the point gk(x) rather than from g^(x) since presumably gMx ) is closer to the solution than gk(x). After using Eq. (40), one would employ Eq. (35

  7. Single grating x-ray imaging for dynamic biological systems

    NASA Astrophysics Data System (ADS)

    Morgan, Kaye S.; Paganin, David M.; Parsons, David W.; Donnelley, Martin; Yagi, Naoto; Uesugi, Kentaro; Suzuki, Yoshio; Takeuchi, Akihisa; Siu, Karen K. W.

    2012-07-01

    Biomedical studies are already benefiting from the excellent contrast offered by phase contrast x-ray imaging, but live imaging work presents several challenges. Living samples make it particularly difficult to achieve high resolution, sensitive phase contrast images, as exposures must be short and cannot be repeated. We therefore present a single-exposure, high-flux method of differential phase contrast imaging [1, 2, 3] in the context of imaging live airways for Cystic Fibrosis (CF) treatment assessment [4]. The CF study seeks to non-invasively observe the liquid lining the airways, which should increase in depth in response to effective treatments. Both high spatial resolution and sensitivity are required in order to track micron size changes in a liquid that is not easily differentiated from the tissue on which it lies. Our imaging method achieves these goals by using a single attenuation grating or grid as a reference pattern, and analyzing how the sample deforms the pattern to quantitatively retrieve the phase depth of the sample. The deformations are mapped at each pixel in the image using local cross-correlations comparing each 'sample and pattern' image with a reference 'pattern only' image taken before the sample is introduced. This produces a differential phase image, which may be integrated to give the sample phase depth.

  8. Multi-object segmentation framework using deformable models for medical imaging analysis.

    PubMed

    Namías, Rafael; D'Amato, Juan Pablo; Del Fresno, Mariana; Vénere, Marcelo; Pirró, Nicola; Bellemare, Marc-Emmanuel

    2016-08-01

    Segmenting structures of interest in medical images is an important step in different tasks such as visualization, quantitative analysis, simulation, and image-guided surgery, among several other clinical applications. Numerous segmentation methods have been developed in the past three decades for extraction of anatomical or functional structures on medical imaging. Deformable models, which include the active contour models or snakes, are among the most popular methods for image segmentation combining several desirable features such as inherent connectivity and smoothness. Even though different approaches have been proposed and significant work has been dedicated to the improvement of such algorithms, there are still challenging research directions as the simultaneous extraction of multiple objects and the integration of individual techniques. This paper presents a novel open-source framework called deformable model array (DMA) for the segmentation of multiple and complex structures of interest in different imaging modalities. While most active contour algorithms can extract one region at a time, DMA allows integrating several deformable models to deal with multiple segmentation scenarios. Moreover, it is possible to consider any existing explicit deformable model formulation and even to incorporate new active contour methods, allowing to select a suitable combination in different conditions. The framework also introduces a control module that coordinates the cooperative evolution of the snakes and is able to solve interaction issues toward the segmentation goal. Thus, DMA can implement complex object and multi-object segmentations in both 2D and 3D using the contextual information derived from the model interaction. These are important features for several medical image analysis tasks in which different but related objects need to be simultaneously extracted. Experimental results on both computed tomography and magnetic resonance imaging show that the proposed

  9. The Grism Lens-Amplified Survey from Space (GLASS). XI. Detection of C IV in Multiple Images of the z = 6.11 Lyα Emitter behind RXC J2248.7-4431

    NASA Astrophysics Data System (ADS)

    Schmidt, K. B.; Huang, K.-H.; Treu, T.; Hoag, A.; Bradač, M.; Henry, A. L.; Jones, T. A.; Mason, C.; Malkan, M.; Morishita, T.; Pentericci, L.; Trenti, M.; Vulcani, B.; Wang, X.

    2017-04-01

    The C III] and C IV rest-frame UV emission lines are powerful probes of the ionization states of galaxies. They have furthermore been suggested as alternatives for spectroscopic redshift confirmation of objects at the epoch of reionization (z> 6), where the most frequently used redshift indicator, Lyα, is attenuated by the high fraction of neutral hydrogen in the intergalactic medium. However, currently only very few confirmations of carbon UV lines at these high redshifts exist, making it challenging to quantify these claims. Here, we present the detection of C IV λλ1548, 1551 Å in Hubble Space Telescope slitless grism spectroscopy obtained by GLASS of a Lyα emitter at z = 6.11 multiply imaged by the massive foreground galaxy cluster RXC J2248.7-4431. The C IV emission is detected at the 3σ-5σ level in two images of the source, with marginal detection in two other images. We do not detect significant C III]λλ1907, 1909 Å emission implying an equivalent width {{EW}}{{C}{{III}}]}< 20 Å (1σ) and {{C}} {{IV}}/{{C}} {{III}}> 0.7 (2σ). Combined with limits on the rest-frame UV flux from the He II λ1640 Å emission line and the O III]λλ1661, 1666 Å doublet, we put constraints on the metallicity and the ionization state of the galaxy. The estimated line ratios and equivalent widths do not support a scenario where an AGN is responsible for ionizing the carbon atoms. SED fits, including nebular emission lines, imply a source with a mass of log(M/M ⊙) ˜ 9, SFR of around 10 M ⊙ yr-1, and a young stellar population < 50 {Myr} old. The source shows a stronger ionizing radiation field than objects with detected C IV emission at z< 2 and adds to the growing sample of low-mass (log(M/M ⊙) ≲ 9) galaxies at the epoch of reionization with strong radiation fields from star formation.

  10. Single-shot three-dimensional reconstruction based on structured light line pattern

    NASA Astrophysics Data System (ADS)

    Wang, ZhenZhou; Yang, YongMing

    2018-07-01

    Reconstruction of the object by single-shot is of great importance in many applications, in which the object is moving or its shape is non-rigid and changes irregularly. In this paper, we propose a single-shot structured light 3D imaging technique that calculates the phase map from the distorted line pattern. This technique makes use of the image processing techniques to segment and cluster the projected structured light line pattern from one single captured image. The coordinates of the clustered lines are extracted to form a low-resolution phase matrix which is then transformed to full-resolution phase map by spline interpolation. The 3D shape of the object is computed from the full-resolution phase map and the 2D camera coordinates. Experimental results show that the proposed method was able to reconstruct the three-dimensional shape of the object robustly from one single image.

  11. ALLFlight: detection of moving objects in IR and ladar images

    NASA Astrophysics Data System (ADS)

    Doehler, H.-U.; Peinecke, Niklas; Lueken, Thomas; Schmerwitz, Sven

    2013-05-01

    Supporting a helicopter pilot during landing and takeoff in degraded visual environment (DVE) is one of the challenges within DLR's project ALLFlight (Assisted Low Level Flight and Landing on Unprepared Landing Sites). Different types of sensors (TV, Infrared, mmW radar and laser radar) are mounted onto DLR's research helicopter FHS (flying helicopter simulator) for gathering different sensor data of the surrounding world. A high performance computer cluster architecture acquires and fuses all the information to get one single comprehensive description of the outside situation. While both TV and IR cameras deliver images with frame rates of 25 Hz or 30 Hz, Ladar and mmW radar provide georeferenced sensor data with only 2 Hz or even less. Therefore, it takes several seconds to detect or even track potential moving obstacle candidates in mmW or Ladar sequences. Especially if the helicopter is flying with higher speed, it is very important to minimize the detection time of obstacles in order to initiate a re-planning of the helicopter's mission timely. Applying feature extraction algorithms on IR images in combination with data fusion algorithms of extracted features and Ladar data can decrease the detection time appreciably. Based on real data from flight tests, the paper describes applied feature extraction methods for moving object detection, as well as data fusion techniques for combining features from TV/IR and Ladar data.

  12. Object-related activity revealed by functional magnetic resonance imaging in human occipital cortex.

    PubMed Central

    Malach, R; Reppas, J B; Benson, R R; Kwong, K K; Jiang, H; Kennedy, W A; Ledden, P J; Brady, T J; Rosen, B R; Tootell, R B

    1995-01-01

    The stages of integration leading from local feature analysis to object recognition were explored in human visual cortex by using the technique of functional magnetic resonance imaging. Here we report evidence for object-related activation. Such activation was located at the lateral-posterior aspect of the occipital lobe, just abutting the posterior aspect of the motion-sensitive area MT/V5, in a region termed the lateral occipital complex (LO). LO showed preferential activation to images of objects, compared to a wide range of texture patterns. This activation was not caused by a global difference in the Fourier spatial frequency content of objects versus texture images, since object images produced enhanced LO activation compared to textures matched in power spectra but randomized in phase. The preferential activation to objects also could not be explained by different patterns of eye movements: similar levels of activation were observed when subjects fixated on the objects and when they scanned the objects with their eyes. Additional manipulations such as spatial frequency filtering and a 4-fold change in visual size did not affect LO activation. These results suggest that the enhanced responses to objects were not a manifestation of low-level visual processing. A striking demonstration that activity in LO is uniquely correlated to object detectability was produced by the "Lincoln" illusion, in which blurring of objects digitized into large blocks paradoxically increases their recognizability. Such blurring led to significant enhancement of LO activation. Despite the preferential activation to objects, LO did not seem to be involved in the final, "semantic," stages of the recognition process. Thus, objects varying widely in their recognizability (e.g., famous faces, common objects, and unfamiliar three-dimensional abstract sculptures) activated it to a similar degree. These results are thus evidence for an intermediate link in the chain of processing stages

  13. D Imaging for Museum Artefacts: a Portable Test Object for Heritage and Museum Documentation of Small Objects

    NASA Astrophysics Data System (ADS)

    Hess, M.; Robson, S.

    2012-07-01

    3D colour image data generated for the recording of small museum objects and archaeological finds are highly variable in quality and fitness for purpose. Whilst current technology is capable of extremely high quality outputs, there are currently no common standards or applicable guidelines in either the museum or engineering domain suited to scientific evaluation, understanding and tendering for 3D colour digital data. This paper firstly explains the rationale towards and requirements for 3D digital documentation in museums. Secondly it describes the design process, development and use of a new portable test object suited to sensor evaluation and the provision of user acceptance metrics. The test object is specifically designed for museums and heritage institutions and includes known surface and geometric properties which support quantitative and comparative imaging on different systems. The development for a supporting protocol will allow object reference data to be included in the data processing workflow with specific reference to conservation and curation.

  14. System for Thermal Imaging of Hot Moving Objects

    NASA Technical Reports Server (NTRS)

    Weinstein, Leonard; Hundley, Jason

    2007-01-01

    The High Altitude/Re-Entry Vehicle Infrared Imaging (HARVII) system is a portable instrumentation system for tracking and thermal imaging of a possibly distant and moving object. The HARVII is designed specifically for measuring the changing temperature distribution on a space shuttle as it reenters the atmosphere. The HARVII system or other systems based on the design of the HARVII system could also be used for such purposes as determining temperature distributions in fires, on volcanoes, and on surfaces of hot models in wind tunnels. In yet another potential application, the HARVII or a similar system would be used to infer atmospheric pollution levels from images of the Sun acquired at multiple wavelengths over regions of interest. The HARVII system includes the Ratio Intensity Thermography System (RITS) and a tracking subsystem that keeps the RITS aimed at the moving object of interest. The subsystem of primary interest here is the RITS (see figure), which acquires and digitizes images of the same scene at different wavelengths in rapid succession. Assuming that the time interval between successive measurements is short enough that temperatures do not change appreciably, the digitized image data at the different wavelengths are processed to extract temperatures according to the principle of ratio-intensity thermography: The temperature at a given location in a scene is inferred from the ratios between or among intensities of infrared radiation from that location at two or more wavelengths. This principle, based on the Stefan-Boltzmann equation for the intensity of electromagnetic radiation as a function of wavelength and temperature, is valid as long as the observed body is a gray or black body and there is minimal atmospheric absorption of radiation.

  15. Robust selectivity to two-object images in human visual cortex

    PubMed Central

    Agam, Yigal; Liu, Hesheng; Papanastassiou, Alexander; Buia, Calin; Golby, Alexandra J.; Madsen, Joseph R.; Kreiman, Gabriel

    2010-01-01

    SUMMARY We can recognize objects in a fraction of a second in spite of the presence of other objects [1–3]. The responses in macaque areas V4 and inferior temporal cortex [4–15] to a neuron’s preferred stimuli are typically suppressed by the addition of a second object within the receptive field (see however [16, 17]). How can this suppression be reconciled with rapid visual recognition in complex scenes? One option is that certain “special categories” are unaffected by other objects [18] but this leaves the problem unsolved for other categories. Another possibility is that serial attentional shifts help ameliorate the problem of distractor objects [19–21]. Yet, psychophysical studies [1–3], scalp recordings [1] and neurophysiological recordings [14, 16, 22–24], suggest that the initial sweep of visual processing contains a significant amount of information. We recorded intracranial field potentials in human visual cortex during presentation of flashes of two-object images. Visual selectivity from temporal cortex during the initial ~200 ms was largely robust to the presence of other objects. We could train linear decoders on the responses to isolated objects and decode information in two-object images. These observations are compatible with parallel, hierarchical and feed-forward theories of rapid visual recognition [25] and may provide a neural substrate to begin to unravel rapid recognition in natural scenes. PMID:20417105

  16. A Marker-Based Approach for the Automated Selection of a Single Segmentation from a Hierarchical Set of Image Segmentations

    NASA Technical Reports Server (NTRS)

    Tarabalka, Y.; Tilton, J. C.; Benediktsson, J. A.; Chanussot, J.

    2012-01-01

    The Hierarchical SEGmentation (HSEG) algorithm, which combines region object finding with region object clustering, has given good performances for multi- and hyperspectral image analysis. This technique produces at its output a hierarchical set of image segmentations. The automated selection of a single segmentation level is often necessary. We propose and investigate the use of automatically selected markers for this purpose. In this paper, a novel Marker-based HSEG (M-HSEG) method for spectral-spatial classification of hyperspectral images is proposed. Two classification-based approaches for automatic marker selection are adapted and compared for this purpose. Then, a novel constrained marker-based HSEG algorithm is applied, resulting in a spectral-spatial classification map. Three different implementations of the M-HSEG method are proposed and their performances in terms of classification accuracies are compared. The experimental results, presented for three hyperspectral airborne images, demonstrate that the proposed approach yields accurate segmentation and classification maps, and thus is attractive for remote sensing image analysis.

  17. Foreign object detection and removal to improve automated analysis of chest radiographs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hogeweg, Laurens; Sanchez, Clara I.; Melendez, Jaime

    2013-07-15

    Purpose: Chest radiographs commonly contain projections of foreign objects, such as buttons, brassier clips, jewellery, or pacemakers and wires. The presence of these structures can substantially affect the output of computer analysis of these images. An automated method is presented to detect, segment, and remove foreign objects from chest radiographs.Methods: Detection is performed using supervised pixel classification with a kNN classifier, resulting in a probability estimate per pixel to belong to a projected foreign object. Segmentation is performed by grouping and post-processing pixels with a probability above a certain threshold. Next, the objects are replaced by texture inpainting.Results: The methodmore » is evaluated in experiments on 257 chest radiographs. The detection at pixel level is evaluated with receiver operating characteristic analysis on pixels within the unobscured lung fields and an A{sub z} value of 0.949 is achieved. Free response operator characteristic analysis is performed at the object level, and 95.6% of objects are detected with on average 0.25 false positive detections per image. To investigate the effect of removing the detected objects through inpainting, a texture analysis system for tuberculosis detection is applied to images with and without pathology and with and without foreign object removal. Unprocessed, the texture analysis abnormality score of normal images with foreign objects is comparable to those with pathology. After removing foreign objects, the texture score of normal images with and without foreign objects is similar, while abnormal images, whether they contain foreign objects or not, achieve on average higher scores.Conclusions: The authors conclude that removal of foreign objects from chest radiographs is feasible and beneficial for automated image analysis.« less

  18. Adaptive foveated single-pixel imaging with dynamic supersampling

    PubMed Central

    Phillips, David B.; Sun, Ming-Jie; Taylor, Jonathan M.; Edgar, Matthew P.; Barnett, Stephen M.; Gibson, Graham M.; Padgett, Miles J.

    2017-01-01

    In contrast to conventional multipixel cameras, single-pixel cameras capture images using a single detector that measures the correlations between the scene and a set of patterns. However, these systems typically exhibit low frame rates, because to fully sample a scene in this way requires at least the same number of correlation measurements as the number of pixels in the reconstructed image. To mitigate this, a range of compressive sensing techniques have been developed which use a priori knowledge to reconstruct images from an undersampled measurement set. Here, we take a different approach and adopt a strategy inspired by the foveated vision found in the animal kingdom—a framework that exploits the spatiotemporal redundancy of many dynamic scenes. In our system, a high-resolution foveal region tracks motion within the scene, yet unlike a simple zoom, every frame delivers new spatial information from across the entire field of view. This strategy rapidly records the detail of quickly changing features in the scene while simultaneously accumulating detail of more slowly evolving regions over several consecutive frames. This architecture provides video streams in which both the resolution and exposure time spatially vary and adapt dynamically in response to the evolution of the scene. The degree of local frame rate enhancement is scene-dependent, but here, we demonstrate a factor of 4, thereby helping to mitigate one of the main drawbacks of single-pixel imaging techniques. The methods described here complement existing compressive sensing approaches and may be applied to enhance computational imagers that rely on sequential correlation measurements. PMID:28439538

  19. An image focusing means by using an opaque object to diffract x-rays

    DOEpatents

    Sommargren, Gary E.; Weaver, H. Joseph

    1991-01-01

    The invention provides a method and apparatus for focusing and imaging x-rays. An opaque sphere is used as a diffractive imaging element to diffract x-rays from an object so that the divergent x-ray wavefronts are transformed into convergent wavefronts and are brought to focus to form an image of the object with a large depth of field.

  20. The Evolution of Ly-alpha Emitting Galaxies Between z = 2.1 and z = 3.l

    NASA Technical Reports Server (NTRS)

    Ciardullo, Robin; Gronwall,Caryl; Wolf, Christopher; McCathran, Emily; Bond, Nicholas A.; Gawiser, Eric; Guaita, Lucia; Feldmeier, John J.; Treister, Ezequiel; Padilla, Nelson; hide

    2011-01-01

    We describe the results of a new, wide-field survey for z= 3.1 Ly-alpha emission-line galaxies (LAEs) in the Extended Chandra Deep Field South (ECDF-S). By using a nearly top-hat 5010 Angstrom filter and complementary broadband photometry from the MUSYC survey, we identify a complete sample of 141 objects with monochromatic fluxes brighter than 2.4E-17 ergs/cm^2/s and observers-frame equivalent widths greater than 80 Angstroms (i.e., 20 Angstroms in the rest-frame of Ly-alpha). The bright-end of this dataset is dominated by x-ray sources and foreground objects with GALEX detections, but when these interlopers are removed, we are still left with a sample of 130 LAE candidates, 39 of which have spectroscopic confirmations. This sample overlaps the set of objects found in an earlier ECDF-S survey, but due to our filter's redder bandpass, it also includes 68 previously uncataloged sources. We confirm earlier measurements of the z=3.1 LAE emission-line luminosity function, and show that an apparent anti-correlation between equivalent width and continuum brightness is likely due to the effect of correlated errors in our heteroskedastic dataset. Finally, we compare the properties of z=3.1 LAEs to LAEs found at z=2.1. We show that in the approximately 1 Gyr after z approximately 3, the LAE luminosity function evolved significantly, with L * fading by approximately 0.4 mag, the number density of sources with L greater than 1.5E42 ergs/s declining by approximately 50%, and the equivalent width scalelength contracting from 70^{+7}_{-5} Angstroms to 50^{+9}_{-6} Angstroms. When combined with literature results, our observations demonstrate that over the redshift range z approximately 0 to z approximately 4, LAEs contain less than approximately 10% of the star-formation rate density of the universe.

  1. Targeted Single-Shot Methods for Diffusion-Weighted Imaging in the Kidneys

    PubMed Central

    Jin, Ning; Deng, Jie; Zhang, Longjiang; Zhang, Zhuoli; Lu, Guangming; Omary, Reed A.; Larson, Andrew C.

    2011-01-01

    Purpose To investigate the feasibility of combining the inner-volume-imaging (IVI) technique with single-shot diffusion-weighted (DW) spin-echo echo-planar imaging (SE-EPI) and DW-SPLICE (split acquisition of fast spin-echo) sequences for renal DW imaging. Materials and Methods Renal DW imaging was performed in 10 healthy volunteers using single-shot DW-SE-EPI, DW-SPLICE, targeted-DW-SE-EPI and targeted-DW-SPLICE. We compared the quantitative diffusion measurement accuracy and image quality of these targeted-DW-SE-EPI and targeted DW-SPLICE methods with conventional full FOV DW-SE-EPI and DW-SPLICE measurements in phantoms and normal volunteers. Results Compared with full FOV DW-SE-EPI and DW-SPLICE methods, targeted-DW-SE-EPI and targeted-DW-SPLICE approaches produced images of superior overall quality with fewer artifacts, less distortion and reduced spatial blurring in both phantom and volunteer studies. The ADC values measured with each of the four methods were similar and in agreement with previously published data. There were no statistically significant differences between the ADC values and intra-voxel incoherent motion (IVIM) measurements in the kidney cortex and medulla using single-shot DW-SE-EPI, targeted-DW-EPI and targeted-DW-SPLICE (p > 0.05). Conclusion Compared with full-FOV DW imaging methods, targeted-DW-SE-EPI and targeted-DW-SPLICE techniques reduced image distortion and artifacts observed in the single-shot DW-SE-EPI images, reduced blurring in DW-SPLICE images and produced comparable quantitative DW and IVIM measurements to those produced with conventional full-FOV approaches. PMID:21591023

  2. Exploiting core knowledge for visual object recognition.

    PubMed

    Schurgin, Mark W; Flombaum, Jonathan I

    2017-03-01

    Humans recognize thousands of objects, and with relative tolerance to variable retinal inputs. The acquisition of this ability is not fully understood, and it remains an area in which artificial systems have yet to surpass people. We sought to investigate the memory process that supports object recognition. Specifically, we investigated the association of inputs that co-occur over short periods of time. We tested the hypothesis that human perception exploits expectations about object kinematics to limit the scope of association to inputs that are likely to have the same token as a source. In several experiments we exposed participants to images of objects, and we then tested recognition sensitivity. Using motion, we manipulated whether successive encounters with an image took place through kinematics that implied the same or a different token as the source of those encounters. Images were injected with noise, or shown at varying orientations, and we included 2 manipulations of motion kinematics. Across all experiments, memory performance was better for images that had been previously encountered with kinematics that implied a single token. A model-based analysis similarly showed greater memory strength when images were shown via kinematics that implied a single token. These results suggest that constraints from physics are built into the mechanisms that support memory about objects. Such constraints-often characterized as 'Core Knowledge'-are known to support perception and cognition broadly, even in young infants. But they have never been considered as a mechanism for memory with respect to recognition. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  3. Single-image-based Rain Detection and Removal via CNN

    NASA Astrophysics Data System (ADS)

    Chen, Tianyi; Fu, Chengzhou

    2018-04-01

    The quality of the image is degraded by rain streaks, which have negative impact when we extract image features for many visual tasks, such as feature extraction for classification and recognition, tracking, surveillance and autonomous navigation. Hence, it is necessary to detect and remove rain streaks from single images, which is a challenging problem since we have no spatial-temporal information of rain streaks compared to the dynamic video stream. Inspired by the priori that the rain streaks have almost the same feature, such as the direction or the thickness, although they are in different types of real-world images. The paper aims at proposing an effective convolutional neural network (CNN) to detect and remove rain streaks from single image. Two models of synthesized rainy image, linear additive composite model (LACM model) and screen blend model (SCM model), are considered in this paper. The main idea is that it is easier for our CNN network to find the mapping between the rainy image and rain streaks than between the rainy image and clean image. The reason is that rain streaks have fixed features, but clean images have various features. The experiments show that the designed CNN network outperforms state-of-the-art approaches on both synthesized and real-world images, which indicates the effectiveness of our proposed framework.

  4. Single Quantum Dot with Microlens and 3D-Printed Micro-objective as Integrated Bright Single-Photon Source

    PubMed Central

    2017-01-01

    Integrated single-photon sources with high photon-extraction efficiency are key building blocks for applications in the field of quantum communications. We report on a bright single-photon source realized by on-chip integration of a deterministic quantum dot microlens with a 3D-printed multilens micro-objective. The device concept benefits from a sophisticated combination of in situ 3D electron-beam lithography to realize the quantum dot microlens and 3D femtosecond direct laser writing for creation of the micro-objective. In this way, we obtain a high-quality quantum device with broadband photon-extraction efficiency of (40 ± 4)% and high suppression of multiphoton emission events with g(2)(τ = 0) < 0.02. Our results highlight the opportunities that arise from tailoring the optical properties of quantum emitters using integrated optics with high potential for the further development of plug-and-play fiber-coupled single-photon sources. PMID:28670600

  5. Vision-based object detection and recognition system for intelligent vehicles

    NASA Astrophysics Data System (ADS)

    Ran, Bin; Liu, Henry X.; Martono, Wilfung

    1999-01-01

    Recently, a proactive crash mitigation system is proposed to enhance the crash avoidance and survivability of the Intelligent Vehicles. Accurate object detection and recognition system is a prerequisite for a proactive crash mitigation system, as system component deployment algorithms rely on accurate hazard detection, recognition, and tracking information. In this paper, we present a vision-based approach to detect and recognize vehicles and traffic signs, obtain their information, and track multiple objects by using a sequence of color images taken from a moving vehicle. The entire system consist of two sub-systems, the vehicle detection and recognition sub-system and traffic sign detection and recognition sub-system. Both of the sub- systems consist of four models: object detection model, object recognition model, object information model, and object tracking model. In order to detect potential objects on the road, several features of the objects are investigated, which include symmetrical shape and aspect ratio of a vehicle and color and shape information of the signs. A two-layer neural network is trained to recognize different types of vehicles and a parameterized traffic sign model is established in the process of recognizing a sign. Tracking is accomplished by combining the analysis of single image frame with the analysis of consecutive image frames. The analysis of the single image frame is performed every ten full-size images. The information model will obtain the information related to the object, such as time to collision for the object vehicle and relative distance from the traffic sings. Experimental results demonstrated a robust and accurate system in real time object detection and recognition over thousands of image frames.

  6. Quantum phase transitions in spin-1 X X Z chains with rhombic single-ion anisotropy

    NASA Astrophysics Data System (ADS)

    Ren, Jie; Wang, Yimin; You, Wen-Long

    2018-04-01

    We explore numerically the inverse participation ratios in the ground state of one-dimensional spin-1 X X Z chains with the rhombic single-ion anisotropy. By employing the techniques of density-matrix renormalization group, effects of the rhombic single-ion anisotropy on various information theoretical measures are investigated, such as the fidelity susceptibility, the quantum coherence, and the entanglement entropy. Their relations with the quantum phase transitions are also analyzed. The phase transitions from the Y -Néel phase to the large-Ex or the Haldane phase can be well characterized by the fidelity susceptibility. The second-order derivative of the ground-state energy indicates all the transitions are of second order. We also find that the quantum coherence, the entanglement entropy, the Schmidt gap, and the inverse participation ratios can be used to detect the critical points of quantum phase transitions. Results drawn from these quantum information observables agree well with each other. Finally we provide a ground-state phase diagram as functions of the exchange anisotropy Δ and the rhombic single-ion anisotropy E .

  7. The iMars WebGIS - Spatio-Temporal Data Queries and Single Image Map Web Services

    NASA Astrophysics Data System (ADS)

    Walter, Sebastian; Steikert, Ralf; Schreiner, Bjoern; Muller, Jan-Peter; van Gasselt, Stephan; Sidiropoulos, Panagiotis; Lanz-Kroechert, Julia

    2017-04-01

    WMS GetMap requests by setting additional TIME parameter values in the request. The values for the parameter represent an interval defined by its lower and upper bounds. As the WMS time standard only supports one time variable, only the start times of the images are considered. If no time values are submitted with the request, the full time range of all images is assumed as the default. Dynamic single image WMS: To compare images from different acquisition times at sites of multiple coverage, we have to load every image as a single WMS layer. Due to the vast amount of single images we need a way to set up the layers in a dynamic way - the map server does not know the images to be served beforehand. We use the MapScript interface to dynamically access MapServer's objects and configure the file name and path of the requested image in the map configuration. The layers are created on-the-fly each representing only one single image. On the frontend side, the vendor-specific WMS request parameter (PRODUCTID) has to be appended to the regular set of WMS parameters. The request is then passed on to the MapScript instance. Web Map Tile Cache: In order to speed up access of the WMS requests, a MapCache instance has been integrated in the pipeline. As it is not aware of the available PDS product IDs which will be queried, the PRODUCTID parameter is configured as an additional dimension of the cache. The WMS request is received by the Apache webserver configured with the MapCache module. If the tile is available in the tile cache, it is immediately commited to the client. If not available, the tile request is forwarded to Apache and the MapScript module. The Python script intercepts the WMS request and extracts the product ID from the parameter chain. It loads the layer object from the map file and appends the file name and path of the inquired image. After some possible further image processing inside the script (stretching, color matching), the request is submitted to the Map

  8. Multiocular image sensor with on-chip beam-splitter and inner meta-micro-lens for single-main-lens stereo camera.

    PubMed

    Koyama, Shinzo; Onozawa, Kazutoshi; Tanaka, Keisuke; Saito, Shigeru; Kourkouss, Sahim Mohamed; Kato, Yoshihisa

    2016-08-08

    We developed multiocular 1/3-inch 2.75-μm-pixel-size 2.1M- pixel image sensors by co-design of both on-chip beam-splitter and 100-nm-width 800-nm-depth patterned inner meta-micro-lens for single-main-lens stereo camera systems. A camera with the multiocular image sensor can capture horizontally one-dimensional light filed by both the on-chip beam-splitter horizontally dividing ray according to incident angle, and the inner meta-micro-lens collecting the divided ray into pixel with small optical loss. Cross-talks between adjacent light field images of a fabricated binocular image sensor and of a quad-ocular image sensor are as low as 6% and 7% respectively. With the selection of two images from one-dimensional light filed images, a selective baseline for stereo vision is realized to view close objects with single-main-lens. In addition, by adding multiple light field images with different ratios, baseline distance can be tuned within an aperture of a main lens. We suggest the electrically selective or tunable baseline stereo vision to reduce 3D fatigue of viewers.

  9. Does a single session of electroconvulsive therapy alter the neural response to emotional faces in depression? A randomised sham-controlled functional magnetic resonance imaging study.

    PubMed

    Miskowiak, Kamilla W; Kessing, Lars V; Ott, Caroline V; Macoveanu, Julian; Harmer, Catherine J; Jørgensen, Anders; Revsbech, Rasmus; Jensen, Hans M; Paulson, Olaf B; Siebner, Hartwig R; Jørgensen, Martin B

    2017-09-01

    Negative neurocognitive bias is a core feature of major depressive disorder that is reversed by pharmacological and psychological treatments. This double-blind functional magnetic resonance imaging study investigated for the first time whether electroconvulsive therapy modulates negative neurocognitive bias in major depressive disorder. Patients with major depressive disorder were randomised to one active ( n=15) or sham electroconvulsive therapy ( n=12). The following day they underwent whole-brain functional magnetic resonance imaging at 3T while viewing emotional faces and performed facial expression recognition and dot-probe tasks. A single electroconvulsive therapy session had no effect on amygdala response to emotional faces. Whole-brain analysis revealed no effects of electroconvulsive therapy versus sham therapy after family-wise error correction at the cluster level, using a cluster-forming threshold of Z>3.1 ( p<0.001) to secure family-wise error <5%. Groups showed no differences in behavioural measures, mood and medication. Exploratory cluster-corrected whole-brain analysis ( Z>2.3; p<0.01) revealed electroconvulsive therapy-induced changes in parahippocampal and superior frontal responses to fearful versus happy faces as well as in fear-specific functional connectivity between amygdala and occipito-temporal regions. Across all patients, greater fear-specific amygdala - occipital coupling correlated with lower fear vigilance. Despite no statistically significant shift in neural response to faces after a single electroconvulsive therapy session, the observed trend changes after a single electroconvulsive therapy session point to an early shift in emotional processing that may contribute to antidepressant effects of electroconvulsive therapy.

  10. Performance assessment of a compressive sensing single-pixel imaging system

    NASA Astrophysics Data System (ADS)

    Du Bosq, Todd W.; Preece, Bradley L.

    2017-04-01

    Conventional sensors measure the light incident at each pixel in a focal plane array. Compressive sensing (CS) involves capturing a smaller number of unconventional measurements from the scene, and then using a companion process to recover the image. CS has the potential to acquire imagery with equivalent information content to a large format array while using smaller, cheaper, and lower bandwidth components. However, the benefits of CS do not come without compromise. The CS architecture chosen must effectively balance between physical considerations, reconstruction accuracy, and reconstruction speed to meet operational requirements. Performance modeling of CS imagers is challenging due to the complexity and nonlinearity of the system and reconstruction algorithm. To properly assess the value of such systems, it is necessary to fully characterize the image quality, including artifacts and sensitivity to noise. Imagery of a two-handheld object target set was collected using an shortwave infrared single-pixel CS camera for various ranges and number of processed measurements. Human perception experiments were performed to determine the identification performance within the trade space. The performance of the nonlinear CS camera was modeled by mapping the nonlinear degradations to an equivalent linear shift invariant model. Finally, the limitations of CS modeling techniques are discussed.

  11. Single-Image Super-Resolution Based on Rational Fractal Interpolation.

    PubMed

    Zhang, Yunfeng; Fan, Qinglan; Bao, Fangxun; Liu, Yifang; Zhang, Caiming

    2018-08-01

    This paper presents a novel single-image super-resolution (SR) procedure, which upscales a given low-resolution (LR) input image to a high-resolution image while preserving the textural and structural information. First, we construct a new type of bivariate rational fractal interpolation model and investigate its analytical properties. This model has different forms of expression with various values of the scaling factors and shape parameters; thus, it can be employed to better describe image features than current interpolation schemes. Furthermore, this model combines the advantages of rational interpolation and fractal interpolation, and its effectiveness is validated through theoretical analysis. Second, we develop a single-image SR algorithm based on the proposed model. The LR input image is divided into texture and non-texture regions, and then, the image is interpolated according to the characteristics of the local structure. Specifically, in the texture region, the scaling factor calculation is the critical step. We present a method to accurately calculate scaling factors based on local fractal analysis. Extensive experiments and comparisons with the other state-of-the-art methods show that our algorithm achieves competitive performance, with finer details and sharper edges.

  12. 3D noise-resistant segmentation and tracking of unknown and occluded objects using integral imaging

    NASA Astrophysics Data System (ADS)

    Aloni, Doron; Jung, Jae-Hyun; Yitzhaky, Yitzhak

    2017-10-01

    Three dimensional (3D) object segmentation and tracking can be useful in various computer vision applications, such as: object surveillance for security uses, robot navigation, etc. We present a method for 3D multiple-object tracking using computational integral imaging, based on accurate 3D object segmentation. The method does not employ object detection by motion analysis in a video as conventionally performed (such as background subtraction or block matching). This means that the movement properties do not significantly affect the detection quality. The object detection is performed by analyzing static 3D image data obtained through computational integral imaging With regard to previous works that used integral imaging data in such a scenario, the proposed method performs the 3D tracking of objects without prior information about the objects in the scene, and it is found efficient under severe noise conditions.

  13. Probing for Binding Regions of the FtsZ Protein Surface through Site-Directed Insertions: Discovery of Fully Functional FtsZ-Fluorescent Proteins

    PubMed Central

    Moore, Desmond A.; Whatley, Zakiya N.; Joshi, Chandra P.; Osawa, Masaki

    2016-01-01

    ABSTRACT FtsZ, a bacterial tubulin homologue, is a cytoskeletal protein that assembles into protofilaments that are one subunit thick. These protofilaments assemble further to form a “Z ring” at the center of prokaryotic cells. The Z ring generates a constriction force on the inner membrane and also serves as a scaffold to recruit cell wall remodeling proteins for complete cell division in vivo. One model of the Z ring proposes that protofilaments associate via lateral bonds to form ribbons; however, lateral bonds are still only hypothetical. To explore potential lateral bonding sites, we probed the surface of Escherichia coli FtsZ by inserting either small peptides or whole fluorescent proteins (FPs). Among the four lateral surfaces on FtsZ protofilaments, we obtained inserts on the front and back surfaces that were functional for cell division. We concluded that these faces are not sites of essential interactions. Inserts at two sites, G124 and R174, located on the left and right surfaces, completely blocked function, and these sites were identified as possible sites for essential lateral interactions. However, the insert at R174 did not interfere with association of protofilaments into sheets and bundles in vitro. Another goal was to find a location within FtsZ that supported insertion of FP reporter proteins while allowing the FtsZ-FPs to function as the sole source of FtsZ. We discovered one internal site, G55-Q56, where several different FPs could be inserted without impairing function. These FtsZ-FPs may provide advances for imaging Z-ring structure by superresolution techniques. IMPORTANCE One model for the Z-ring structure proposes that protofilaments are assembled into ribbons by lateral bonds between FtsZ subunits. Our study excluded the involvement of the front and back faces of the protofilament in essential interactions in vivo but pointed to two potential lateral bond sites, on the right and left sides. We also identified an FtsZ loop where

  14. Polarization-multiplexing ghost imaging

    NASA Astrophysics Data System (ADS)

    Dongfeng, Shi; Jiamin, Zhang; Jian, Huang; Yingjian, Wang; Kee, Yuan; Kaifa, Cao; Chenbo, Xie; Dong, Liu; Wenyue, Zhu

    2018-03-01

    A novel technique for polarization-multiplexing ghost imaging is proposed to simultaneously obtain multiple polarimetric information by a single detector. Here, polarization-division multiplexing speckles are employed for object illumination. The light reflected from the objects is detected by a single-pixel detector. An iterative reconstruction method is used to restore the fused image containing the different polarimetric information by using the weighted sum of the multiplexed speckles based on the correlation coefficients obtained from the detected intensities. Next, clear images of the different polarimetric information are recovered by demultiplexing the fused image. The results clearly demonstrate that the proposed method is effective.

  15. Preliminary research on dual-energy X-ray phase-contrast imaging

    NASA Astrophysics Data System (ADS)

    Han, Hua-Jie; Wang, Sheng-Hao; Gao, Kun; Wang, Zhi-Li; Zhang, Can; Yang, Meng; Zhang, Kai; Zhu, Pei-Ping

    2016-04-01

    Dual-energy X-ray absorptiometry (DEXA) has been widely applied to measure the bone mineral density (BMD) and soft-tissue composition of the human body. However, the use of DEXA is greatly limited for low-Z materials such as soft tissues due to their weak absorption, while X-ray phase-contrast imaging (XPCI) shows significantly improved contrast in comparison with the conventional standard absorption-based X-ray imaging for soft tissues. In this paper, we propose a novel X-ray phase-contrast method to measure the area density of low-Z materials, including a single-energy method and a dual-energy method. The single-energy method is for the area density calculation of one low-Z material, while the dual-energy method aims to calculate the area densities of two low-Z materials simultaneously. Comparing the experimental and simulation results with the theoretical ones, the new method proves to have the potential to replace DEXA in area density measurement. The new method sets the prerequisites for a future precise and low-dose area density calculation method for low-Z materials. Supported by Major State Basic Research Development Program (2012CB825800), Science Fund for Creative Research Groups (11321503) and National Natural Science Foundation of China (11179004, 10979055, 11205189, 11205157)

  16. Repetition Blindness for Natural Images of Objects with Viewpoint Changes

    PubMed Central

    Buffat, Stéphane; Plantier, Justin; Roumes, Corinne; Lorenceau, Jean

    2013-01-01

    When stimuli are repeated in a rapid serial visual presentation (RSVP), observers sometimes fail to report the second occurrence of a target. This phenomenon is referred to as “repetition blindness” (RB). We report an RSVP experiment with photographs in which we manipulated object viewpoints between the first and second occurrences of a target (0°, 45°, or 90° changes), and spatial frequency (SF) content. Natural images were spatially filtered to produce low, medium, or high SF stimuli. RB was observed for all filtering conditions. Surprisingly, for full-spectrum (FS) images, RB increased significantly as the viewpoint reached 90°. For filtered images, a similar pattern of results was found for all conditions except for medium SF stimuli. These findings suggest that object recognition in RSVP are subtended by viewpoint-specific representations for all spatial frequencies except medium ones. PMID:23346069

  17. Combining Imaging and Non-Imaging Observations for Improved Space-Object Identification

    DTIC Science & Technology

    2011-09-27

    Optical and Digital Superresolution Early in the project, we expolited Fisher information (FI) to characterize the extent of spatial-frequency...extrapolation beyond the diffraction-limited optical bandwidth when the support of the object is known a priori. This support-assisted optical superresolution ...both digital (DSR) and optical superresolution (OSR). Indeed, by analyzing a se- quence of sub-pixel-shifted undersampled images one can show the

  18. Multiple defocused coherent diffraction imaging: method for simultaneously reconstructing objects and probe using X-ray free-electron lasers.

    PubMed

    Hirose, Makoto; Shimomura, Kei; Suzuki, Akihiro; Burdet, Nicolas; Takahashi, Yukio

    2016-05-30

    The sample size must be less than the diffraction-limited focal spot size of the incident beam in single-shot coherent X-ray diffraction imaging (CXDI) based on a diffract-before-destruction scheme using X-ray free electron lasers (XFELs). This is currently a major limitation preventing its wider applications. We here propose multiple defocused CXDI, in which isolated objects are sequentially illuminated with a divergent beam larger than the objects and the coherent diffraction pattern of each object is recorded. This method can simultaneously reconstruct both objects and a probe from the coherent X-ray diffraction patterns without any a priori knowledge. We performed a computer simulation of the prposed method and then successfully demonstrated it in a proof-of-principle experiment at SPring-8. The prposed method allows us to not only observe broad samples but also characterize focused XFEL beams.

  19. Learning-Based Object Identification and Segmentation Using Dual-Energy CT Images for Security.

    PubMed

    Martin, Limor; Tuysuzoglu, Ahmet; Karl, W Clem; Ishwar, Prakash

    2015-11-01

    In recent years, baggage screening at airports has included the use of dual-energy X-ray computed tomography (DECT), an advanced technology for nondestructive evaluation. The main challenge remains to reliably find and identify threat objects in the bag from DECT data. This task is particularly hard due to the wide variety of objects, the high clutter, and the presence of metal, which causes streaks and shading in the scanner images. Image noise and artifacts are generally much more severe than in medical CT and can lead to splitting of objects and inaccurate object labeling. The conventional approach performs object segmentation and material identification in two decoupled processes. Dual-energy information is typically not used for the segmentation, and object localization is not explicitly used to stabilize the material parameter estimates. We propose a novel learning-based framework for joint segmentation and identification of objects directly from volumetric DECT images, which is robust to streaks, noise and variability due to clutter. We focus on segmenting and identifying a small set of objects of interest with characteristics that are learned from training images, and consider everything else as background. We include data weighting to mitigate metal artifacts and incorporate an object boundary field to reduce object splitting. The overall formulation is posed as a multilabel discrete optimization problem and solved using an efficient graph-cut algorithm. We test the method on real data and show its potential for producing accurate labels of the objects of interest without splits in the presence of metal and clutter.

  20. The linac coherent light source single particle imaging road map

    PubMed Central

    Aquila, A.; Barty, A.; Bostedt, C.; Boutet, S.; Carini, G.; dePonte, D.; Drell, P.; Doniach, S.; Downing, K. H.; Earnest, T.; Elmlund, H.; Elser, V.; Gühr, M.; Hajdu, J.; Hastings, J.; Hau-Riege, S. P.; Huang, Z.; Lattman, E. E.; Maia, F. R. N. C.; Marchesini, S.; Ourmazd, A.; Pellegrini, C.; Santra, R.; Schlichting, I.; Schroer, C.; Spence, J. C. H.; Vartanyants, I. A.; Wakatsuki, S.; Weis, W. I.; Williams, G. J.

    2015-01-01

    Intense femtosecond x-ray pulses from free-electron laser sources allow the imaging of individual particles in a single shot. Early experiments at the Linac Coherent Light Source (LCLS) have led to rapid progress in the field and, so far, coherent diffractive images have been recorded from biological specimens, aerosols, and quantum systems with a few-tens-of-nanometers resolution. In March 2014, LCLS held a workshop to discuss the scientific and technical challenges for reaching the ultimate goal of atomic resolution with single-shot coherent diffractive imaging. This paper summarizes the workshop findings and presents the roadmap toward reaching atomic resolution, 3D imaging at free-electron laser sources. PMID:26798801

  1. The linac coherent light source single particle imaging road map

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Aquila, A.; Barty, A.; Bostedt, C.

    Intense femtosecond x-ray pulses from free-electron laser sources allow the imaging of individual particles in a single shot. Early experiments at the Linac Coherent Light Source (LCLS) have led to rapid progress in the field and, so far, coherent diffractive images have been recorded from biological specimens, aerosols, and quantum systems with a few-tens-of-nanometers resolution. In March 2014, LCLS held a workshop to discuss the scientific and technical challenges for reaching the ultimate goal of atomic resolution with single-shot coherent diffractive imaging. This paper summarizes the workshop findings and presents the roadmap toward reaching atomic resolution, 3D imaging at free-electronmore » laser sources.« less

  2. Correlating objective and subjective evaluation of texture appearance with applications to camera phone imaging

    NASA Astrophysics Data System (ADS)

    Phillips, Jonathan B.; Coppola, Stephen M.; Jin, Elaine W.; Chen, Ying; Clark, James H.; Mauer, Timothy A.

    2009-01-01

    Texture appearance is an important component of photographic image quality as well as object recognition. Noise cleaning algorithms are used to decrease sensor noise of digital images, but can hinder texture elements in the process. The Camera Phone Image Quality (CPIQ) initiative of the International Imaging Industry Association (I3A) is developing metrics to quantify texture appearance. Objective and subjective experimental results of the texture metric development are presented in this paper. Eight levels of noise cleaning were applied to ten photographic scenes that included texture elements such as faces, landscapes, architecture, and foliage. Four companies (Aptina Imaging, LLC, Hewlett-Packard, Eastman Kodak Company, and Vista Point Technologies) have performed psychophysical evaluations of overall image quality using one of two methods of evaluation. Both methods presented paired comparisons of images on thin film transistor liquid crystal displays (TFT-LCD), but the display pixel pitch and viewing distance differed. CPIQ has also been developing objective texture metrics and targets that were used to analyze the same eight levels of noise cleaning. The correlation of the subjective and objective test results indicates that texture perception can be modeled with an objective metric. The two methods of psychophysical evaluation exhibited high correlation despite the differences in methodology.

  3. Three-Dimensional Reconstruction from Single Image Base on Combination of CNN and Multi-Spectral Photometric Stereo.

    PubMed

    Lu, Liang; Qi, Lin; Luo, Yisong; Jiao, Hengchao; Dong, Junyu

    2018-03-02

    Multi-spectral photometric stereo can recover pixel-wise surface normal from a single RGB image. The difficulty lies in that the intensity in each channel is the tangle of illumination, albedo and camera response; thus, an initial estimate of the normal is required in optimization-based solutions. In this paper, we propose to make a rough depth estimation using the deep convolutional neural network (CNN) instead of using depth sensors or binocular stereo devices. Since high-resolution ground-truth data is expensive to obtain, we designed a network and trained it with rendered images of synthetic 3D objects. We use the model to predict initial normal of real-world objects and iteratively optimize the fine-scale geometry in the multi-spectral photometric stereo framework. The experimental results illustrate the improvement of the proposed method compared with existing methods.

  4. Three-Dimensional Reconstruction from Single Image Base on Combination of CNN and Multi-Spectral Photometric Stereo

    PubMed Central

    Lu, Liang; Qi, Lin; Luo, Yisong; Jiao, Hengchao; Dong, Junyu

    2018-01-01

    Multi-spectral photometric stereo can recover pixel-wise surface normal from a single RGB image. The difficulty lies in that the intensity in each channel is the tangle of illumination, albedo and camera response; thus, an initial estimate of the normal is required in optimization-based solutions. In this paper, we propose to make a rough depth estimation using the deep convolutional neural network (CNN) instead of using depth sensors or binocular stereo devices. Since high-resolution ground-truth data is expensive to obtain, we designed a network and trained it with rendered images of synthetic 3D objects. We use the model to predict initial normal of real-world objects and iteratively optimize the fine-scale geometry in the multi-spectral photometric stereo framework. The experimental results illustrate the improvement of the proposed method compared with existing methods. PMID:29498703

  5. A single-chain variable fragment intrabody prevents intracellular polymerization of Z α1-antitrypsin while allowing its antiproteinase activity.

    PubMed

    Ordóñez, Adriana; Pérez, Juan; Tan, Lu; Dickens, Jennifer A; Motamedi-Shad, Neda; Irving, James A; Haq, Imran; Ekeowa, Ugo; Marciniak, Stefan J; Miranda, Elena; Lomas, David A

    2015-06-01

    Mutant Z α1-antitrypsin (E342K) accumulates as polymers within the endoplasmic reticulum (ER) of hepatocytes predisposing to liver disease, whereas low levels of circulating Z α1-antitrypsin lead to emphysema by loss of inhibition of neutrophil elastase. The ideal therapy should prevent polymer formation while preserving inhibitory activity. Here we used mAb technology to identify interactors with Z α1-antitrypsin that comply with both requirements. We report the generation of an mAb (4B12) that blocked α1-antitrypsin polymerization in vitro at a 1:1 molar ratio, causing a small increase of the stoichiometry of inhibition for neutrophil elastase. A single-chain variable fragment (scFv) intrabody was generated based on the sequence of mAb4B12. The expression of scFv4B12 within the ER (scFv4B12KDEL) and along the secretory pathway (scFv4B12) reduced the intracellular polymerization of Z α1-antitrypsin by 60%. The scFv4B12 intrabody also increased the secretion of Z α1-antitrypsin that retained inhibitory activity against neutrophil elastase. MAb4B12 recognized a discontinuous epitope probably located in the region of helices A/C/G/H/I and seems to act by altering protein dynamics rather than binding preferentially to the native state. This novel approach could reveal new target sites for small-molecule intervention that may block the transition to aberrant polymers without compromising the inhibitory activity of Z α1-antitrypsin. © FASEB.

  6. Detection of hidden objects using a real-time 3-D millimeter-wave imaging system

    NASA Astrophysics Data System (ADS)

    Rozban, Daniel; Aharon, Avihai; Levanon, Assaf; Abramovich, Amir; Yitzhaky, Yitzhak; Kopeika, N. S.

    2014-10-01

    Millimeter (mm)and sub-mm wavelengths or terahertz (THz) band have several properties that motivate their use in imaging for security applications such as recognition of hidden objects, dangerous materials, aerosols, imaging through walls as in hostage situations, and also in bad weather conditions. There is no known ionization hazard for biological tissue, and atmospheric degradation of THz radiation is relatively low for practical imaging distances. We recently developed a new technology for the detection of THz radiation. This technology is based on very inexpensive plasma neon indicator lamps, also known as Glow Discharge Detector (GDD), that can be used as very sensitive THz radiation detectors. Using them, we designed and constructed a Focal Plane Array (FPA) and obtained recognizable2-dimensional THz images of both dielectric and metallic objects. Using THz wave it is shown here that even concealed weapons made of dielectric material can be detected. An example is an image of a knife concealed inside a leather bag and also under heavy clothing. Three-dimensional imaging using radar methods can enhance those images since it can allow the isolation of the concealed objects from the body and environmental clutter such as nearby furniture or other people. The GDDs enable direct heterodyning between the electric field of the target signal and the reference signal eliminating the requirement for expensive mixers, sources, and Low Noise Amplifiers (LNAs).We expanded the ability of the FPA so that we are able to obtain recognizable 2-dimensional THz images in real time. We show here that the THz detection of objects in three dimensions, using FMCW principles is also applicable in real time. This imaging system is also shown here to be capable of imaging objects from distances allowing standoff detection of suspicious objects and humans from large distances.

  7. Single-Molecule Light-Sheet Imaging of Suspended T Cells.

    PubMed

    Ponjavic, Aleks; McColl, James; Carr, Alexander R; Santos, Ana Mafalda; Kulenkampff, Klara; Lippert, Anna; Davis, Simon J; Klenerman, David; Lee, Steven F

    2018-05-08

    Adaptive immune responses are initiated by triggering of the T cell receptor. Single-molecule imaging based on total internal reflection fluorescence microscopy at coverslip/basal cell interfaces is commonly used to study this process. These experiments have suggested, unexpectedly, that the diffusional behavior and organization of signaling proteins and receptors may be constrained before activation. However, it is unclear to what extent the molecular behavior and cell state is affected by the imaging conditions, i.e., by the presence of a supporting surface. In this study, we implemented single-molecule light-sheet microscopy, which enables single receptors to be directly visualized at any plane in a cell to study protein dynamics and organization in live, resting T cells. The light sheet enabled the acquisition of high-quality single-molecule fluorescence images that were comparable to those of total internal reflection fluorescence microscopy. By comparing the apical and basal surfaces of surface-contacting T cells using single-molecule light-sheet microscopy, we found that most coated-glass surfaces and supported lipid bilayers profoundly affected the diffusion of membrane proteins (T cell receptor and CD45) and that all the surfaces induced calcium influx to various degrees. Our results suggest that, when studying resting T cells, surfaces are best avoided, which we achieve here by suspending cells in agarose. Copyright © 2018. Published by Elsevier Inc.

  8. Preparation of longitudinal sections of hair samples for the analysis of cocaine by MALDI-MS/MS and TOF-SIMS imaging.

    PubMed

    Flinders, Bryn; Cuypers, Eva; Zeijlemaker, Hans; Tytgat, Jan; Heeren, Ron M A

    2015-10-01

    Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) for the analysis of intact hair is a powerful tool for the detection of drugs of abuse in toxicology and forensic applications. Here we present a quick, easy, and reproducible method of preparing longitudinal sections of single hairs. This method improves the accessibility of chemicals embedded in the hair matrix for molecular imaging with mass spectrometry. The images obtained from a single, sectioned hair sample show molecular distributions in the exposed medulla, cortex, and a portion of the cuticle observed as a narrow layer surrounding the cortex. Using MALDI-MS/MS imaging, the distribution of cocaine was observed throughout five longitudinally sectioned drug-user hair samples. The images showed the distribution of the product ion at m/z 182, derived from the precursor ion of cocaine at m/z 304. MetA-SIMS images of longitudinally sectioned hair samples showed a more detailed distribution of cocaine at m/z 304, benzoylecgonine the major metabolite of cocaine at m/z 290 and other drugs such as methadone which was observed at m/z 310. Chronological information of drug intake can be obtained more sensitively. The chronological detail is in hours rather than months, which is of great interest in clinical as well as forensic applications. Copyright © 2015 John Wiley & Sons, Ltd.

  9. [E75, R78 and D82 of Escherichia coli FtsZ are key residues for FtsZ cellular self-assembly and FtsZ-MreB interaction].

    PubMed

    Huo, Yujia; Lu, Qiaonan; Zheng, Xiaowei; Ma, Yuanfang; Lu, Feng

    2016-02-04

    To explore effects of FtsZ mutants FtsZ(E75A), FtsZ(R78G) and FtsZ(D82A) on FtsZ self-assembly and interaction of FtsZ with MreB in Escherichia coli strains. METHODS) We constructed FtsZ and its mutant's plasmids by molecular clone and site-directed mutagenesis methods, and purified targeted proteins by affinity chromatography. QN6(ftsZ::yfp-cat), QN7(tsZ::yfp-cat), QN8(ftsZ(R78G)::yfp-cat) and QN9 (ftsZ(D82A):.:yfp-cat) strains were constructed by linear DNA homologous recombination. We observed cellular localization pattern of FtsZ and its mutants in E. coli by living cell imaging experiments, examined interaction of FtsZ/FtsZ*-FtsZ* and FtsZ/FtsZ*-MreB by Coimmunoprecipitation and bacteria two hybrid, and analyzed assembly characteristics of FtsZ mutants by Light scattering. RESULTS) The Yfp-labeled FtsZ(E75A), FtsZ(R78G) and FtsZ(D82A) mutant proteins failed to assemble into functional Z-ring structure and localize correctly in E. coli strains. Interaction of FtsZ with its mutants, or FtsZ*-FtsZ* and FtsZ*-MreB interaction were weakened or completely disappeared. In addition, in vitro experiments show that E75A, R78G and D82A mutations decreased the polymerization efficiency of FtsZ monomer. FtsZ E75, R78 and D82 are critical amino acids in the assembly, function of FtsZ protein and FtsZ-MreB interaction in E. coli strains.

  10. Object-Oriented Query Language For Events Detection From Images Sequences

    NASA Astrophysics Data System (ADS)

    Ganea, Ion Eugen

    2015-09-01

    In this paper is presented a method to represent the events extracted from images sequences and the query language used for events detection. Using an object oriented model the spatial and temporal relationships between salient objects and also between events are stored and queried. This works aims to unify the storing and querying phases for video events processing. The object oriented language syntax used for events processing allow the instantiation of the indexes classes in order to improve the accuracy of the query results. The experiments were performed on images sequences provided from sport domain and it shows the reliability and the robustness of the proposed language. To extend the language will be added a specific syntax for constructing the templates for abnormal events and for detection of the incidents as the final goal of the research.

  11. A novel snapshot polarimetric imager

    NASA Astrophysics Data System (ADS)

    Wong, Gerald; McMaster, Ciaran; Struthers, Robert; Gorman, Alistair; Sinclair, Peter; Lamb, Robert; Harvey, Andrew R.

    2012-10-01

    Polarimetric imaging (PI) is of increasing importance in determining additional scene information beyond that of conventional images. For very long-range surveillance, image quality is degraded due to turbulence. Furthermore, the high magnification required to create images with sufficient spatial resolution suitable for object recognition and identification require long focal length optical systems. These are incompatible with the size and weight restrictions for aircraft. Techniques which allow detection and recognition of an object at the single pixel level are therefore likely to provide advance warning of approaching threats or long-range object cueing. PI is a technique that has the potential to detect object signatures at the pixel level. Early attempts to develop PI used rotating polarisers (and spectral filters) which recorded sequential polarized images from which the complete Stokes matrix could be derived. This approach has built-in latency between frames and requires accurate registration of consecutive frames to analyze real-time video of moving objects. Alternatively, multiple optical systems and cameras have been demonstrated to remove latency, but this approach increases cost and bulk of the imaging system. In our investigation we present a simplified imaging system that divides an image into two orthogonal polarimetric components which are then simultaneously projected onto a single detector array. Thus polarimetric data is recorded without latency on a single snapshot. We further show that, for pixel-level objects, the data derived from only two orthogonal states (H and V) is sufficient to increase the probability of detection whilst reducing false alarms compared to conventional unpolarised imaging.

  12. Simulations for Improved Imaging of Faint Objects at Maui Space Surveillance Site

    NASA Astrophysics Data System (ADS)

    Holmes, R.; Roggemann, M.; Werth, M.; Lucas, J.; Thompson, D.

    A detailed wave-optics simulation is used in conjunction with advanced post-processing algorithms to explore the trade space between image post-processing and adaptive optics for improved imaging of low signal-to-noise ratio (SNR) targets. Target-based guidestars are required for imaging of most active Earth-orbiting satellites because of restrictions on using laser-backscatter-based guidestars in the direction of such objects. With such target-based guidestars and Maui conditions, it is found that significant reductions in adaptive optics actuator and subaperture density can result in improved imaging of fainter objects. Simulation indicates that elimination of adaptive optics produces sub-optimal results for all of the faint-object cases considered. This research was developed with funding from the Defense Advanced Research Projects Agency (DARPA). The views, opinions, and/or findings expressed are those of the author(s) and should not be interpreted as representing the official views or policies of the Department of Defense or the U.S. Government.

  13. Object detection and imaging with acoustic time reversal mirrors

    NASA Astrophysics Data System (ADS)

    Fink, Mathias

    1993-11-01

    Focusing an acoustic wave on an object of unknown shape through an inhomogeneous medium of any geometrical shape is a challenge in underground detection. Optimal detection and imaging of objects needs the development of such focusing techniques. The use of a time reversal mirror (TRM) represents an original solution to this problem. It realizes in real time a focusing process matched to the object shape, to the geometries of the acoustic interfaces and to the geometries of the mirror. It is a self adaptative technique which compensates for any geometrical distortions of the mirror structure as well as for diffraction and refraction effects through the interfaces. Two real time 64 and 128 channel prototypes have been built in our laboratory and TRM experiments demonstrating the TRM performance through inhomogeneous solid and liquid media are presented. Applications to medical therapy (kidney stone detection and destruction) and to nondestructive testing of metallurgical samples of different geometries are described. Extension of this study to underground detection and imaging will be discussed.

  14. Using object-based image analysis to guide the selection of field sample locations

    USDA-ARS?s Scientific Manuscript database

    One of the most challenging tasks for resource management and research is designing field sampling schemes to achieve unbiased estimates of ecosystem parameters as efficiently as possible. This study focused on the potential of fine-scale image objects from object-based image analysis (OBIA) to be u...

  15. Single Cell Fluorescence Imaging Using Metal Plasmon-Coupled Probe

    PubMed Central

    Zhang, Jian; Fu, Yi; Lakowicz, Joseph R.

    2009-01-01

    This work constitutes the first fluorescent imaging of cells using metal plasmon-coupled probes (PCPs) at single cell resolution. N-(2-Mercapto-propionyl)glycine-coated silver nanoparticles were synthesized by reduction of silver nitrate using sodium borohyride and then succinimidylated via ligand exchange. Alexa Fluor 647-labeled concanavalin A (con A) was chemically bound to the silver particles to make the fluorescent metal plasmon-coupled probes. The fluorescence images were collected using a scanning confocal microscopy. The fluorescence intensity was observed to enhance 7-fold when binding the labeled con A on a single silver particle. PCPs were conjugated on HEK 293 A cells. Imaging results demonstrate that cells labeled by PCPs were 20-fold brighter than those by free labeled con A. PMID:17375898

  16. Extended study on the influence of z-value(s) of single and multicomponent time-temperature integrators on the accuracy of quantitative thermal process assessment.

    PubMed

    Guiavarc'h, Yann P; van Loey, Ann M; Hendrickx, Marc E

    2005-02-01

    The possibilities and limitations of single- and multicomponent time-temperature integrators (TTIs) for evaluating the impact of thermal processes on a target food attribute with a Ztarget value different from the zTTI value(s) of the TTI is far from sufficiently documented. In this study, several thousand time-temperature profiles were generated by heat transfer simulations based on a wide range of product and process thermal parameters and considering a Ztarget value of 10 degrees C and a reference temperature of 121.1 degrees C, both currently used to assess the safety of food sterilization processes. These simulations included 15 different Ztarget=10 degrees CF121.1 degrees C values in the range 3 to 60 min. The integration of the time-temperature profiles with ZTTI values of 5.5 to 20.5 degrees C in steps of 1 degrees C allowed generation of a large database containing for each combination of product and process parameters the correction factor to apply to the process value FmultiTTI, which was derived from a single- or multicomponent TTI, to obtain the target process value 10 degrees CF121.1 degrees C. The table and the graph results clearly demonstrated that multicomponent TTIs with z-values close to 10 degrees C can be used as an extremely efficient approach when a single-component TTI with a z-value of 10 degrees C is not available. In particular, a two-component TTI with z1 and z2 values respectively above and below the Ztarget value (10 degrees C in this study) would be the best option for the development of a TTI to assess the safety of sterilized foods. Whatever process and product parameters are used, such a TTI allows proper evaluation of the process value 10 degrees CF121.1 degrees C.

  17. Video-Rate Confocal Microscopy for Single-Molecule Imaging in Live Cells and Superresolution Fluorescence Imaging

    PubMed Central

    Lee, Jinwoo; Miyanaga, Yukihiro; Ueda, Masahiro; Hohng, Sungchul

    2012-01-01

    There is no confocal microscope optimized for single-molecule imaging in live cells and superresolution fluorescence imaging. By combining the swiftness of the line-scanning method and the high sensitivity of wide-field detection, we have developed a, to our knowledge, novel confocal fluorescence microscope with a good optical-sectioning capability (1.0 μm), fast frame rates (<33 fps), and superior fluorescence detection efficiency. Full compatibility of the microscope with conventional cell-imaging techniques allowed us to do single-molecule imaging with a great ease at arbitrary depths of live cells. With the new microscope, we monitored diffusion motion of fluorescently labeled cAMP receptors of Dictyostelium discoideum at both the basal and apical surfaces and obtained superresolution fluorescence images of microtubules of COS-7 cells at depths in the range 0–85 μm from the surface of a coverglass. PMID:23083712

  18. Single-particle characterization of urban aerosol particles collected in three Korean cites using low-Z electron probe X-ray microanalysis.

    PubMed

    Ro, Chul-Un; Kim, HyeKyeong; Oh, Keun-Young; Yea, Sun Kyung; Lee, Chong Bum; Jang, Meongdo; Van Grieken, René

    2002-11-15

    A recently developed single-particle analytical technique, called low-Z electron probe X-ray microanalysis (low-Z EPMA), was applied to characterize urban aerosol particles collected in three cities of Korea (Seoul, CheongJu, and ChunCheon) on single days in the winter of 1999. In this study, it is clearly demonstrated that the low-Z EPMA technique can provide detailed and quantitative information on the chemical composition of particles in the urban atmosphere. The collected aerosol particles were analyzed and classified on the basis of their chemical species. Various types of particles were identified, such as soil-derived, carbonaceous, marine-originated, and anthropogenic particles. In the sample collected in Seoul, carbonaceous, aluminosilicates, silicon dioxide, and calcium carbonate aerosol particles were abundantly encountered. In the CheongJu and ChunCheon samples, carbonaceous, aluminosilicates, reacted sea salts, and ammonium sulfate aerosol particles were often seen. However, in the CheongJu sample, ammonium sulfate particles were the most abundant in the fine fraction. Also, calcium sulfate and nitrate particles were significantly observed. In the ChunCheon sample, organic particles were the most abundant in the fine fraction. Also, sodium nitrate particles were seen at high levels. The ChunCheon sample seemed to be strongly influenced by sea-salt aerosols originating from the Yellow Sea, which is located about 115 km away from the city.

  19. Galaxy evolution in the densest environments: HST imaging

    NASA Astrophysics Data System (ADS)

    Jorgensen, Inger

    2013-10-01

    We propose to process in a consistent fashion all available HST/ACS and WFC3 imaging of seven rich clusters of galaxies at z=1.2-1.6. The clusters are part of our larger project aimed at constraining models for galaxy evolution in dense environments from observations of stellar populations in rich z=1.2-2 galaxy clusters. The main objective is to establish the star formation {SF} history and structural evolution over this epoch during which large changes in SF rates and galaxy structure are expected to take place in cluster galaxies.The observational data required to meet our main objective are deep HST imaging and high S/N spectroscopy of individual cluster members. The HST imaging already exists for the seven rich clusters at z=1.2-1.6 included in this archive proposal. However, the data have not been consistently processed to derive colors, magnitudes, sizes and morphological parameters for all potential cluster members bright enough to be suitable for spectroscopic observations with 8-m class telescopes. We propose to carry out this processing and make all derived parameters publicly available. We will use the parameters derived from the HST imaging to {1} study the structural evolution of the galaxies, {2} select clusters and galaxies for spectroscopic observations, and {3} use the photometry and spectroscopy together for a unified analysis aimed at the SF history and structural changes. The analysis will also utilize data from the Gemini/HST Cluster Galaxy Project, which covers rich clusters at z=0.2-1.0 and for which we have similar HST imaging and high S/N spectroscopy available.

  20. Integrating fuzzy object based image analysis and ant colony optimization for road extraction from remotely sensed images

    NASA Astrophysics Data System (ADS)

    Maboudi, Mehdi; Amini, Jalal; Malihi, Shirin; Hahn, Michael

    2018-04-01

    Updated road network as a crucial part of the transportation database plays an important role in various applications. Thus, increasing the automation of the road extraction approaches from remote sensing images has been the subject of extensive research. In this paper, we propose an object based road extraction approach from very high resolution satellite images. Based on the object based image analysis, our approach incorporates various spatial, spectral, and textural objects' descriptors, the capabilities of the fuzzy logic system for handling the uncertainties in road modelling, and the effectiveness and suitability of ant colony algorithm for optimization of network related problems. Four VHR optical satellite images which are acquired by Worldview-2 and IKONOS satellites are used in order to evaluate the proposed approach. Evaluation of the extracted road networks shows that the average completeness, correctness, and quality of the results can reach 89%, 93% and 83% respectively, indicating that the proposed approach is applicable for urban road extraction. We also analyzed the sensitivity of our algorithm to different ant colony optimization parameter values. Comparison of the achieved results with the results of four state-of-the-art algorithms and quantifying the robustness of the fuzzy rule set demonstrate that the proposed approach is both efficient and transferable to other comparable images.

  1. Statistics of Visual Responses to Image Object Stimuli from Primate AIT Neurons to DNN Neurons.

    PubMed

    Dong, Qiulei; Wang, Hong; Hu, Zhanyi

    2018-02-01

    Under the goal-driven paradigm, Yamins et al. ( 2014 ; Yamins & DiCarlo, 2016 ) have shown that by optimizing only the final eight-way categorization performance of a four-layer hierarchical network, not only can its top output layer quantitatively predict IT neuron responses but its penultimate layer can also automatically predict V4 neuron responses. Currently, deep neural networks (DNNs) in the field of computer vision have reached image object categorization performance comparable to that of human beings on ImageNet, a data set that contains 1.3 million training images of 1000 categories. We explore whether the DNN neurons (units in DNNs) possess image object representational statistics similar to monkey IT neurons, particularly when the network becomes deeper and the number of image categories becomes larger, using VGG19, a typical and widely used deep network of 19 layers in the computer vision field. Following Lehky, Kiani, Esteky, and Tanaka ( 2011 , 2014 ), where the response statistics of 674 IT neurons to 806 image stimuli are analyzed using three measures (kurtosis, Pareto tail index, and intrinsic dimensionality), we investigate the three issues in this letter using the same three measures: (1) the similarities and differences of the neural response statistics between VGG19 and primate IT cortex, (2) the variation trends of the response statistics of VGG19 neurons at different layers from low to high, and (3) the variation trends of the response statistics of VGG19 neurons when the numbers of stimuli and neurons increase. We find that the response statistics on both single-neuron selectivity and population sparseness of VGG19 neurons are fundamentally different from those of IT neurons in most cases; by increasing the number of neurons in different layers and the number of stimuli, the response statistics of neurons at different layers from low to high do not substantially change; and the estimated intrinsic dimensionality values at the low

  2. High resolution FTIR imaging provides automated discrimination and detection of single malaria parasite infected erythrocytes on glass.

    PubMed

    Perez-Guaita, David; Andrew, Dean; Heraud, Philip; Beeson, James; Anderson, David; Richards, Jack; Wood, Bayden R

    2016-06-23

    New highly sensitive tools for malaria diagnostics are urgently needed to enable the detection of infection in asymptomatic carriers and patients with low parasitemia. In pursuit of a highly sensitive diagnostic tool that can identify parasite infections at the single cell level, we have been exploring Fourier transform infrared (FTIR) microscopy using a Focal Plane Array (FPA) imaging detector. Here we report for the first time the application of a new optic configuration developed by Agilent that incorporates 25× condenser and objective Cassegrain optics with a high numerical aperture (NA = 0.81) along with additional high magnification optics within the microscope to provide 0.66 micron pixel resolution (total IR system magnification of 61×) to diagnose malaria parasites at the single cell level on a conventional glass microscope slide. The high quality images clearly resolve the parasite's digestive vacuole demonstrating sub-cellular resolution using this approach. Moreover, we have developed an algorithm that first detects the cells in the infrared image, and secondly extracts the average spectrum. The average spectrum is then run through a model based on Partial Least Squares-Discriminant Analysis (PLS-DA), which diagnoses unequivocally the infected from normal cells. The high quality images, and the fact this measurement can be achieved without a synchrotron source on a conventional glass slide, shows promise as a potential gold standard for malaria detection at the single cell level.

  3. Biomarkers and Biological Spectral Imaging

    DTIC Science & Technology

    2001-01-23

    Image t iZ ~ Rotator SSteping r De m oo ontroler Frmegabe (single ams) ... Software -= • . ... PCCm ue Data acquisition Rotator control PC Co puterImage...the depth of bum injury", Bums, 7, pp. 197-202, 1981. 7. R. A. De Blasi, M. Cope, C. Elwell, F. Safoue and M. Ferrari, "Noninvasive measurement of...nicotinamide adenine dinucleotide (NADH), oxidized flavin adenine dinucleotide (FAD) and porphyrins. A number of studies have shown that the measured

  4. Depression and quality of life for women in single-parent and nuclear families.

    PubMed

    Landero Hernández, René; Estrada Aranda, Benito; González Ramírez, Mónica Teresa

    2009-05-01

    This is a cross-sectional study which objectives are 1) to determine the predictors for perceived quality of life and 2) to analyze the differences between women from single-parent families and bi-parent families, about their quality of life, depression and familiar income. We worked with a non-probabilistic sample of 140 women from Monterrey, N.L, Mexico, 107 are from bi-parent families and 33 from single parent families. Some of the results show that women from single-parent families have lower quality of life (Z = -2.224, p = .026), lower income (Z = -2.727, p = .006) and greater depression (Z = -6.143, p = .001) than women from bi-parental families. The perceived quality of life's predictors, using a multiple regression model (n = 140) were depression, income and number of children, those variables explaining 25.4% of variance.

  5. Cell biochemistry studied by single-molecule imaging.

    PubMed

    Mashanov, G I; Nenasheva, T A; Peckham, M; Molloy, J E

    2006-11-01

    Over the last decade, there have been remarkable developments in live-cell imaging. We can now readily observe individual protein molecules within living cells and this should contribute to a systems level understanding of biological pathways. Direct observation of single fluorophores enables several types of molecular information to be gathered. Temporal and spatial trajectories enable diffusion constants and binding kinetics to be deduced, while analyses of fluorescence lifetime, intensity, polarization or spectra give chemical and conformational information about molecules in their cellular context. By recording the spatial trajectories of pairs of interacting molecules, formation of larger molecular complexes can be studied. In the future, multicolour and multiparameter imaging of single molecules in live cells will be a powerful analytical tool for systems biology. Here, we discuss measurements of single-molecule mobility and residency at the plasma membrane of live cells. Analysis of diffusional paths at the plasma membrane gives information about its physical properties and measurement of temporal trajectories enables rates of binding and dissociation to be derived. Meanwhile, close scrutiny of individual fluorophore trajectories enables ideas about molecular dimerization and oligomerization related to function to be tested directly.

  6. Mosaicing of single plane illumination microscopy images using groupwise registration and fast content-based image fusion

    NASA Astrophysics Data System (ADS)

    Preibisch, Stephan; Rohlfing, Torsten; Hasak, Michael P.; Tomancak, Pavel

    2008-03-01

    Single Plane Illumination Microscopy (SPIM; Huisken et al., Nature 305(5686):1007-1009, 2004) is an emerging microscopic technique that enables live imaging of large biological specimens in their entirety. By imaging the living biological sample from multiple angles SPIM has the potential to achieve isotropic resolution throughout even relatively large biological specimens. For every angle, however, only a relatively shallow section of the specimen is imaged with high resolution, whereas deeper regions appear increasingly blurred. In order to produce a single, uniformly high resolution image, we propose here an image mosaicing algorithm that combines state of the art groupwise image registration for alignment with content-based image fusion to prevent degrading of the fused image due to regional blurring of the input images. For the registration stage, we introduce an application-specific groupwise transformation model that incorporates per-image as well as groupwise transformation parameters. We also propose a new fusion algorithm based on Gaussian filters, which is substantially faster than fusion based on local image entropy. We demonstrate the performance of our mosaicing method on data acquired from living embryos of the fruit fly, Drosophila, using four and eight angle acquisitions.

  7. [An object-oriented remote sensing image segmentation approach based on edge detection].

    PubMed

    Tan, Yu-Min; Huai, Jian-Zhu; Tang, Zhong-Shi

    2010-06-01

    Satellite sensor technology endorsed better discrimination of various landscape objects. Image segmentation approaches to extracting conceptual objects and patterns hence have been explored and a wide variety of such algorithms abound. To this end, in order to effectively utilize edge and topological information in high resolution remote sensing imagery, an object-oriented algorithm combining edge detection and region merging is proposed. Susan edge filter is firstly applied to the panchromatic band of Quickbird imagery with spatial resolution of 0.61 m to obtain the edge map. Thanks to the resulting edge map, a two-phrase region-based segmentation method operates on the fusion image from panchromatic and multispectral Quickbird images to get the final partition result. In the first phase, a quad tree grid consisting of squares with sides parallel to the image left and top borders agglomerates the square subsets recursively where the uniform measure is satisfied to derive image object primitives. Before the merger of the second phrase, the contextual and spatial information, (e. g., neighbor relationship, boundary coding) of the resulting squares are retrieved efficiently by means of the quad tree structure. Then a region merging operation is performed with those primitives, during which the criterion for region merging integrates edge map and region-based features. This approach has been tested on the QuickBird images of some site in Sanxia area and the result is compared with those of ENVI Zoom Definiens. In addition, quantitative evaluation of the quality of segmentation results is also presented. Experiment results demonstrate stable convergence and efficiency.

  8. Assessment of illumination conditions in a single-pixel imaging configuration

    NASA Astrophysics Data System (ADS)

    Garoi, Florin; Udrea, Cristian; Damian, Cristian; Logofǎtu, Petre C.; Colţuc, Daniela

    2016-12-01

    Single-pixel imaging based on multiplexing is a promising technique, especially in applications where 2D detectors or raster scanning imaging are not readily applicable. With this method, Hadamard masks are projected on a spatial light modulator to encode an incident scene and a signal is recorded at the photodiode detector for each of these masks. Ultimately, the image is reconstructed on the computer by applying the inverse transform matrix. Thus, various algorithms were optimized and several spatial light modulators already characterized for such a task. This work analyses the imaging quality of such a single-pixel arrangement, when various illumination conditions are used. More precisely, the main comparison is made between coherent and incoherent ("white light") illumination and between two multiplexing methods, namely Hadamard and Scanning. The quality of the images is assessed by calculating their SNR, using two relations. The results show better images are obtained with "white light" illumination for the first method and coherent one for the second.

  9. CLASH: DISCOVERY OF A BRIGHT z {approx_equal} 6.2 DWARF GALAXY QUADRUPLY LENSED BY MACS J0329.6-0211

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Zitrin, A.; Moustakas, J.; Bradley, L.

    2012-03-15

    We report the discovery of a z{sub phot} = 6.18{sup +0.05}{sub -0.07} (95% confidence level) dwarf galaxy, lensed into four images by the galaxy cluster MACS J0329.6-0211 (z{sub l} = 0.45). The galaxy is observed as a high-redshift dropout in HST/ACS/WFC3 CLASH and Spitzer/IRAC imaging. Its redshift is securely determined due to a clear detection of the Lyman break in the 18-band photometry, making this galaxy one of the highest-redshift multiply lensed objects known to date with an observed magnitude of F125W =24.00 {+-} 0.04 AB mag for its most magnified image. We also present the first strong-lensing analysis ofmore » this cluster uncovering 15 additional multiply imaged candidates of five lower-redshift sources spanning the range z{sub s} {approx_equal} 2-4. The mass model independently supports the high photometric redshift and reveals magnifications of 11.6{sup +8.9}{sub -4.1}, 17.6{sup +6.2}{sub -3.9}, 3.9{sup +3.0}{sub -1.7}, and 3.7{sup +1.3}{sub -0.2}, respectively, for the four images of the high-redshift galaxy. By delensing the most magnified image we construct an image of the source with a physical resolution of {approx}200 pc when the universe was {approx}0.9 Gyr old, where the z {approx_equal} 6.2 galaxy occupies a source-plane area of approximately 2.2 kpc{sup 2}. Modeling the observed spectral energy distribution using population synthesis models, we find a demagnified stellar mass of {approx}10{sup 9} M{sub Sun }, subsolar metallicity (Z/Z{sub Sun} {approx} 0.5), low dust content (A{sub V} {approx} 0.1 mag), a demagnified star formation rate (SFR) of {approx}3.2 M{sub Sun} yr{sup -1}, and a specific SFR of {approx}3.4 Gyr{sup -1}, all consistent with the properties of local dwarf galaxies.« less

  10. Combined X-ray CT and mass spectrometry for biomedical imaging applications

    NASA Astrophysics Data System (ADS)

    Schioppa, E., Jr.; Ellis, S.; Bruinen, A. L.; Visser, J.; Heeren, R. M. A.; Uher, J.; Koffeman, E.

    2014-04-01

    Imaging technologies play a key role in many branches of science, especially in biology and medicine. They provide an invaluable insight into both internal structure and processes within a broad range of samples. There are many techniques that allow one to obtain images of an object. Different techniques are based on the analysis of a particular sample property by means of a dedicated imaging system, and as such, each imaging modality provides the researcher with different information. The use of multimodal imaging (imaging with several different techniques) can provide additional and complementary information that is not possible when employing a single imaging technique alone. In this study, we present for the first time a multi-modal imaging technique where X-ray computerized tomography (CT) is combined with mass spectrometry imaging (MSI). While X-ray CT provides 3-dimensional information regarding the internal structure of the sample based on X-ray absorption coefficients, MSI of thin sections acquired from the same sample allows the spatial distribution of many elements/molecules, each distinguished by its unique mass-to-charge ratio (m/z), to be determined within a single measurement and with a spatial resolution as low as 1 μm or even less. The aim of the work is to demonstrate how molecular information from MSI can be spatially correlated with 3D structural information acquired from X-ray CT. In these experiments, frozen samples are imaged in an X-ray CT setup using Medipix based detectors equipped with a CO2 cooled sample holder. Single projections are pre-processed before tomographic reconstruction using a signal-to-thickness calibration. In the second step, the object is sliced into thin sections (circa 20 μm) that are then imaged using both matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and secondary ion (SIMS) mass spectrometry, where the spatial distribution of specific molecules within the sample is determined. The

  11. Single-photon imaging in complementary metal oxide semiconductor processes

    PubMed Central

    Charbon, E.

    2014-01-01

    This paper describes the basics of single-photon counting in complementary metal oxide semiconductors, through single-photon avalanche diodes (SPADs), and the making of miniaturized pixels with photon-counting capability based on SPADs. Some applications, which may take advantage of SPAD image sensors, are outlined, such as fluorescence-based microscopy, three-dimensional time-of-flight imaging and biomedical imaging, to name just a few. The paper focuses on architectures that are best suited to those applications and the trade-offs they generate. In this context, architectures are described that efficiently collect the output of single pixels when designed in large arrays. Off-chip readout circuit requirements are described for a variety of applications in physics, medicine and the life sciences. Owing to the dynamic nature of SPADs, designs featuring a large number of SPADs require careful analysis of the target application for an optimal use of silicon real estate and of limited readout bandwidth. The paper also describes the main trade-offs involved in architecting such chips and the solutions adopted with focus on scalability and miniaturization. PMID:24567470

  12. The influence of familiar characters and other appealing images on young children's preference for low-quality objects.

    PubMed

    Danovitch, Judith H; Mills, Candice M

    2017-09-01

    This study examines the factors underlying young children's preference for products bearing a familiar character's image. Three-year-olds (N = 92) chose between low-quality objects with images on or near the objects and high-quality objects without images. Children showed stronger preferences for damaged objects bearing images of a preferred familiar character than for objects bearing images of a preferred colour star, and they showed weak preferences for damaged objects with the character near, but not on, the object. The results suggest that children's preference for low-quality products bearing character images is driven by prior exposure to characters, and not only by the act of identifying a favourite. Statement of contribution What is already known on this subject? Children are exposed to characters in the media and on products such as clothing and school supplies. Products featuring familiar characters appeal to preschool children, even if they are of low quality. What does this study add? Three-year-olds prefer damaged objects with an image of a favourite character over plain undamaged objects. Children's preference is not solely a function of having identified a favourite image or of attentional cues. © 2017 The British Psychological Society.

  13. Object-based modeling, identification, and labeling of medical images for content-based retrieval by querying on intervals of attribute values

    NASA Astrophysics Data System (ADS)

    Thies, Christian; Ostwald, Tamara; Fischer, Benedikt; Lehmann, Thomas M.

    2005-04-01

    The classification and measuring of objects in medical images is important in radiological diagnostics and education, especially when using large databases as knowledge resources, for instance a picture archiving and communication system (PACS). The main challenge is the modeling of medical knowledge and the diagnostic context to label the sought objects. This task is referred to as closing the semantic gap between low-level pixel information and high level application knowledge. This work describes an approach which allows labeling of a-priori unknown objects in an intuitive way. Our approach consists of four main components. At first an image is completely decomposed into all visually relevant partitions on different scales. This provides a hierarchical organized set of regions. Afterwards, for each of the obtained regions a set of descriptive features is computed. In this data structure objects are represented by regions with characteristic attributes. The actual object identification is the formulation of a query. It consists of attributes on which intervals are defined describing those regions that correspond to the sought objects. Since the objects are a-priori unknown, they are described by a medical expert by means of an intuitive graphical user interface (GUI). This GUI is the fourth component. It enables complex object definitions by browsing the data structure and examinating the attributes to formulate the query. The query is executed and if the sought objects have not been identified its parameterization is refined. By using this heuristic approach, object models for hand radiographs have been developed to extract bones from a single hand in different anatomical contexts. This demonstrates the applicability of the labeling concept. By using a rule for metacarpal bones on a series of 105 images, this type of bone could be retrieved with a precision of 0.53 % and a recall of 0.6%.

  14. A Single-Photon Avalanche Diode Array for Fluorescence Lifetime Imaging Microscopy.

    PubMed

    Schwartz, David Eric; Charbon, Edoardo; Shepard, Kenneth L

    2008-11-21

    We describe the design, characterization, and demonstration of a fully integrated single-photon avalanche diode (SPAD) imager for use in time-resolved fluorescence imaging. The imager consists of a 64-by-64 array of active SPAD pixels and an on-chip time-to-digital converter (TDC) based on a delay-locked loop (DLL) and calibrated interpolators. The imager can perform both standard time-correlated single-photon counting (TCSPC) and an alternative gated-window detection useful for avoiding pulse pile-up when measuring bright signal levels. To illustrate the use of the imager, we present measurements of the decay lifetimes of fluorescent dyes of several types with a timing resolution of 350 ps.

  15. A Single-Photon Avalanche Diode Array for Fluorescence Lifetime Imaging Microscopy

    PubMed Central

    Schwartz, David Eric; Charbon, Edoardo; Shepard, Kenneth L.

    2013-01-01

    We describe the design, characterization, and demonstration of a fully integrated single-photon avalanche diode (SPAD) imager for use in time-resolved fluorescence imaging. The imager consists of a 64-by-64 array of active SPAD pixels and an on-chip time-to-digital converter (TDC) based on a delay-locked loop (DLL) and calibrated interpolators. The imager can perform both standard time-correlated single-photon counting (TCSPC) and an alternative gated-window detection useful for avoiding pulse pile-up when measuring bright signal levels. To illustrate the use of the imager, we present measurements of the decay lifetimes of fluorescent dyes of several types with a timing resolution of 350 ps. PMID:23976789

  16. Single-photon imager based on a superconducting nanowire delay line

    NASA Astrophysics Data System (ADS)

    Zhao, Qing-Yuan; Zhu, Di; Calandri, Niccolò; Dane, Andrew E.; McCaughan, Adam N.; Bellei, Francesco; Wang, Hao-Zhu; Santavicca, Daniel F.; Berggren, Karl K.

    2017-03-01

    Detecting spatial and temporal information of individual photons is critical to applications in spectroscopy, communication, biological imaging, astronomical observation and quantum-information processing. Here we demonstrate a scalable single-photon imager using a single continuous superconducting nanowire that is not only a single-photon detector but also functions as an efficient microwave delay line. In this context, photon-detection pulses are guided in the nanowire and enable the readout of the position and time of photon-absorption events from the arrival times of the detection pulses at the nanowire's two ends. Experimentally, we slowed down the velocity of pulse propagation to ∼2% of the speed of light in free space. In a 19.7 mm long nanowire that meandered across an area of 286 × 193 μm2, we were able to resolve ∼590 effective pixels with a temporal resolution of 50 ps (full width at half maximum). The nanowire imager presents a scalable approach for high-resolution photon imaging in space and time.

  17. Three-dimensional motion-picture imaging of dynamic object by parallel-phase-shifting digital holographic microscopy using an inverted magnification optical system

    NASA Astrophysics Data System (ADS)

    Fukuda, Takahito; Shinomura, Masato; Xia, Peng; Awatsuji, Yasuhiro; Nishio, Kenzo; Matoba, Osamu

    2017-04-01

    We constructed a parallel-phase-shifting digital holographic microscopy (PPSDHM) system using an inverted magnification optical system, and succeeded in three-dimensional (3D) motion-picture imaging for 3D displacement of a microscopic object. In the PPSDHM system, the inverted and afocal magnification optical system consisted of a microscope objective (16.56 mm focal length and 0.25 numerical aperture) and a convex lens (300 mm focal length and 82 mm aperture diameter). A polarization-imaging camera was used to record multiple phase-shifted holograms with a single-shot exposure. We recorded an alum crystal, sinking down in aqueous solution of alum, by the constructed PPSDHM system at 60 frames/s for about 20 s and reconstructed high-quality 3D motion-picture image of the crystal. Then, we calculated amounts of displacement of the crystal from the amounts in the focus plane and the magnifications of the magnification optical system, and obtained the 3D trajectory of the crystal by that amounts.

  18. Industrial-scale separation of high-purity single-chirality single-wall carbon nanotubes for biological imaging

    PubMed Central

    Yomogida, Yohei; Tanaka, Takeshi; Zhang, Minfang; Yudasaka, Masako; Wei, Xiaojun; Kataura, Hiromichi

    2016-01-01

    Single-chirality, single-wall carbon nanotubes are desired due to their inherent physical properties and performance characteristics. Here, we demonstrate a chromatographic separation method based on a newly discovered chirality-selective affinity between carbon nanotubes and a gel containing a mixture of the surfactants. In this system, two different selectivities are found: chiral-angle selectivity and diameter selectivity. Since the chirality of nanotubes is determined by the chiral angle and diameter, combining these independent selectivities leads to high-resolution single-chirality separation with milligram-scale throughput and high purity. Furthermore, we present efficient vascular imaging of mice using separated single-chirality (9,4) nanotubes. Due to efficient absorption and emission, blood vessels can be recognized even with the use of ∼100-fold lower injected dose than the reported value for pristine nanotubes. Thus, 1 day of separation provides material for up to 15,000 imaging experiments, which is acceptable for industrial use. PMID:27350127

  19. Image Segmentation Method Using Fuzzy C Mean Clustering Based on Multi-Objective Optimization

    NASA Astrophysics Data System (ADS)

    Chen, Jinlin; Yang, Chunzhi; Xu, Guangkui; Ning, Li

    2018-04-01

    Image segmentation is not only one of the hottest topics in digital image processing, but also an important part of computer vision applications. As one kind of image segmentation algorithms, fuzzy C-means clustering is an effective and concise segmentation algorithm. However, the drawback of FCM is that it is sensitive to image noise. To solve the problem, this paper designs a novel fuzzy C-mean clustering algorithm based on multi-objective optimization. We add a parameter λ to the fuzzy distance measurement formula to improve the multi-objective optimization. The parameter λ can adjust the weights of the pixel local information. In the algorithm, the local correlation of neighboring pixels is added to the improved multi-objective mathematical model to optimize the clustering cent. Two different experimental results show that the novel fuzzy C-means approach has an efficient performance and computational time while segmenting images by different type of noises.

  20. Hierarchical rendering of trees from precomputed multi-layer z-buffers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Max, N.

    1996-02-01

    Chen and Williams show how precomputed z-buffer images from different fixed viewing positions can be reprojected to produce an image for a new viewpoint. Here images are precomputed for twigs and branches at various levels in the hierarchical structure of a tree, and adaptively combined, depending on the position of the new viewpoint. The precomputed images contain multiple z levels to avoid missing pixels in the reconstruction, subpixel masks for anti-aliasing, and colors and normals for shading after reprojection.

  1. Single Photon Counting Performance and Noise Analysis of CMOS SPAD-Based Image Sensors.

    PubMed

    Dutton, Neale A W; Gyongy, Istvan; Parmesan, Luca; Henderson, Robert K

    2016-07-20

    SPAD-based solid state CMOS image sensors utilising analogue integrators have attained deep sub-electron read noise (DSERN) permitting single photon counting (SPC) imaging. A new method is proposed to determine the read noise in DSERN image sensors by evaluating the peak separation and width (PSW) of single photon peaks in a photon counting histogram (PCH). The technique is used to identify and analyse cumulative noise in analogue integrating SPC SPAD-based pixels. The DSERN of our SPAD image sensor is exploited to confirm recent multi-photon threshold quanta image sensor (QIS) theory. Finally, various single and multiple photon spatio-temporal oversampling techniques are reviewed.

  2. Single Photon Counting Performance and Noise Analysis of CMOS SPAD-Based Image Sensors

    PubMed Central

    Dutton, Neale A. W.; Gyongy, Istvan; Parmesan, Luca; Henderson, Robert K.

    2016-01-01

    SPAD-based solid state CMOS image sensors utilising analogue integrators have attained deep sub-electron read noise (DSERN) permitting single photon counting (SPC) imaging. A new method is proposed to determine the read noise in DSERN image sensors by evaluating the peak separation and width (PSW) of single photon peaks in a photon counting histogram (PCH). The technique is used to identify and analyse cumulative noise in analogue integrating SPC SPAD-based pixels. The DSERN of our SPAD image sensor is exploited to confirm recent multi-photon threshold quanta image sensor (QIS) theory. Finally, various single and multiple photon spatio-temporal oversampling techniques are reviewed. PMID:27447643

  3. Video-rate confocal microscopy for single-molecule imaging in live cells and superresolution fluorescence imaging.

    PubMed

    Lee, Jinwoo; Miyanaga, Yukihiro; Ueda, Masahiro; Hohng, Sungchul

    2012-10-17

    There is no confocal microscope optimized for single-molecule imaging in live cells and superresolution fluorescence imaging. By combining the swiftness of the line-scanning method and the high sensitivity of wide-field detection, we have developed a, to our knowledge, novel confocal fluorescence microscope with a good optical-sectioning capability (1.0 μm), fast frame rates (<33 fps), and superior fluorescence detection efficiency. Full compatibility of the microscope with conventional cell-imaging techniques allowed us to do single-molecule imaging with a great ease at arbitrary depths of live cells. With the new microscope, we monitored diffusion motion of fluorescently labeled cAMP receptors of Dictyostelium discoideum at both the basal and apical surfaces and obtained superresolution fluorescence images of microtubules of COS-7 cells at depths in the range 0-85 μm from the surface of a coverglass. Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  4. Imaging of platelet-derived growth factor receptor β expression in glioblastoma xenografts using affibody molecule 111In-DOTA-Z09591.

    PubMed

    Tolmachev, Vladimir; Varasteh, Zohreh; Honarvar, Hadis; Hosseinimehr, Seyed Jalal; Eriksson, Olof; Jonasson, Per; Frejd, Fredrik Y; Abrahmsen, Lars; Orlova, Anna

    2014-02-01

    The overexpression and excessive signaling of platelet-derived growth factor receptor β (PDGFRβ) has been detected in cancers, atherosclerosis, and a variety of fibrotic diseases. Radionuclide in vivo visualization of PDGFRβ expression might help to select PDGFRβ targeting treatment for these diseases. The goal of this study was to evaluate the feasibility of in vivo radionuclide imaging of PDGFRβ expression using an Affibody molecule, a small nonimmunoglobulin affinity protein. The PDGFRβ-binding Z09591 Affibody molecule was site-specifically conjugated with a maleimido derivative of DOTA and labeled with (111)In. Targeting of the PDGFRβ-expressing U-87 MG glioblastoma cell line using (111)In-DOTA-Z09591 was evaluated in vitro and in vivo. DOTA-Z09591 was stably labeled with (111)In with preserved specific binding to PDGFRβ-expressing cells in vitro. The dissociation constant for (111)In-DOTA-Z09591 binding to U-87 MG cells was determined to be 92 ± 10 pM. In mice bearing U-87 MG xenografts, the tumor uptake of (111)In-DOTA-Z09591 was 7.2 ± 2.4 percentage injected dose per gram and the tumor-to-blood ratio was 28 ± 14 at 2 h after injection. In vivo receptor saturation experiments demonstrated that targeting of U-87 MG xenografts in mice was PDGFRβ-specific. U-87 MG xenografts were clearly visualized using small-animal SPECT/CT at 3 h after injection. This study demonstrates the feasibility of in vivo visualization of PDGFRβ-expressing xenografts using an Affibody molecule. Further development of radiolabeled Affibody molecules might provide a useful clinical imaging tool for PDGFRβ expression during various pathologic conditions.

  5. Multispectral image analysis for object recognition and classification

    NASA Astrophysics Data System (ADS)

    Viau, C. R.; Payeur, P.; Cretu, A.-M.

    2016-05-01

    Computer and machine vision applications are used in numerous fields to analyze static and dynamic imagery in order to assist or automate decision-making processes. Advancements in sensor technologies now make it possible to capture and visualize imagery at various wavelengths (or bands) of the electromagnetic spectrum. Multispectral imaging has countless applications in various fields including (but not limited to) security, defense, space, medical, manufacturing and archeology. The development of advanced algorithms to process and extract salient information from the imagery is a critical component of the overall system performance. The fundamental objective of this research project was to investigate the benefits of combining imagery from the visual and thermal bands of the electromagnetic spectrum to improve the recognition rates and accuracy of commonly found objects in an office setting. A multispectral dataset (visual and thermal) was captured and features from the visual and thermal images were extracted and used to train support vector machine (SVM) classifiers. The SVM's class prediction ability was evaluated separately on the visual, thermal and multispectral testing datasets.

  6. Graphene metamaterial spatial light modulator for infrared single pixel imaging.

    PubMed

    Fan, Kebin; Suen, Jonathan Y; Padilla, Willie J

    2017-10-16

    High-resolution and hyperspectral imaging has long been a goal for multi-dimensional data fusion sensing applications - of interest for autonomous vehicles and environmental monitoring. In the long wave infrared regime this quest has been impeded by size, weight, power, and cost issues, especially as focal-plane array detector sizes increase. Here we propose and experimentally demonstrated a new approach based on a metamaterial graphene spatial light modulator (GSLM) for infrared single pixel imaging. A frequency-division multiplexing (FDM) imaging technique is designed and implemented, and relies entirely on the electronic reconfigurability of the GSLM. We compare our approach to the more common raster-scan method and directly show FDM image frame rates can be 64 times faster with no degradation of image quality. Our device and related imaging architecture are not restricted to the infrared regime, and may be scaled to other bands of the electromagnetic spectrum. The study presented here opens a new approach for fast and efficient single pixel imaging utilizing graphene metamaterials with novel acquisition strategies.

  7. (3Z,6Z,9Z,12Z,15Z)-Pentacosapentaene and (Z) -11-Hexadecenyl Acetate: sex attractant blend for Dioryctria amatella (Lepidoptera: Pyralidae).

    Treesearch

    Daniel Miller; Jocelyn Millar; Alex Mangini; Christopher Crowe; Gary Grant

    2010-01-01

    In 2006-2008, we tested (3Z,6Z,9Z,12Z,15Z)-pentacosapentaene (pentaene) with the pheromone components (Z)-11-hexadecenyl acetate (Z11-16:Ac) and (Z)-9-tetradecenyl acetate (Z9-14:Ac), as sex attractants for four sympatric species of coneworms, Dioryctria Zeller (Lepidoptera: Pyralidae) in slash (Pinus elliottii Engelm.) and...

  8. An object tracking method based on guided filter for night fusion image

    NASA Astrophysics Data System (ADS)

    Qian, Xiaoyan; Wang, Yuedong; Han, Lei

    2016-01-01

    Online object tracking is a challenging problem as it entails learning an effective model to account for appearance change caused by intrinsic and extrinsic factors. In this paper, we propose a novel online object tracking with guided image filter for accurate and robust night fusion image tracking. Firstly, frame difference is applied to produce the coarse target, which helps to generate observation models. Under the restriction of these models and local source image, guided filter generates sufficient and accurate foreground target. Then accurate boundaries of the target can be extracted from detection results. Finally timely updating for observation models help to avoid tracking shift. Both qualitative and quantitative evaluations on challenging image sequences demonstrate that the proposed tracking algorithm performs favorably against several state-of-art methods.

  9. Orientational imaging of a single plasmonic nanoparticle using dark-field hyperspectral imaging

    NASA Astrophysics Data System (ADS)

    Mehta, Nishir; Mahigir, Amirreza; Veronis, Georgios; Gartia, Manas Ranjan

    2017-08-01

    Orientation of plasmonic nanostructures is an important feature in many nanoscale applications such as catalyst, biosensors DNA interactions, protein detections, hotspot of surface enhanced Raman spectroscopy (SERS), and fluorescence resonant energy transfer (FRET) experiments. However, due to diffraction limit, it is challenging to obtain the exact orientation of the nanostructure using standard optical microscope. Hyperspectral Imaging Microscopy is a state-of-the-art visualization technology that combines modern optics with hyperspectral imaging and computer system to provide the identification and quantitative spectral analysis of nano- and microscale structures. In this work, initially we use transmitted dark field imaging technique to locate single nanoparticle on a glass substrate. Then we employ hyperspectral imaging technique at the same spot to investigate orientation of single nanoparticle. No special tagging or staining of nanoparticle has been done, as more likely required in traditional microscopy techniques. Different orientations have been identified by carefully understanding and calibrating shift in spectral response from each different orientations of similar sized nanoparticles. Wavelengths recorded are between 300 nm to 900 nm. The orientations measured by hyperspectral microscopy was validated using finite difference time domain (FDTD) electrodynamics calculations and scanning electron microscopy (SEM) analysis. The combination of high resolution nanometer-scale imaging techniques and the modern numerical modeling capacities thus enables a meaningful advance in our knowledge of manipulating and fabricating shaped nanostructures. This work will advance our understanding of the behavior of small nanoparticle clusters useful for sensing, nanomedicine, and surface sciences.

  10. Minimal camera networks for 3D image based modeling of cultural heritage objects.

    PubMed

    Alsadik, Bashar; Gerke, Markus; Vosselman, George; Daham, Afrah; Jasim, Luma

    2014-03-25

    3D modeling of cultural heritage objects like artifacts, statues and buildings is nowadays an important tool for virtual museums, preservation and restoration. In this paper, we introduce a method to automatically design a minimal imaging network for the 3D modeling of cultural heritage objects. This becomes important for reducing the image capture time and processing when documenting large and complex sites. Moreover, such a minimal camera network design is desirable for imaging non-digitally documented artifacts in museums and other archeological sites to avoid disturbing the visitors for a long time and/or moving delicate precious objects to complete the documentation task. The developed method is tested on the Iraqi famous statue "Lamassu". Lamassu is a human-headed winged bull of over 4.25 m in height from the era of Ashurnasirpal II (883-859 BC). Close-range photogrammetry is used for the 3D modeling task where a dense ordered imaging network of 45 high resolution images were captured around Lamassu with an object sample distance of 1 mm. These images constitute a dense network and the aim of our study was to apply our method to reduce the number of images for the 3D modeling and at the same time preserve pre-defined point accuracy. Temporary control points were fixed evenly on the body of Lamassu and measured by using a total station for the external validation and scaling purpose. Two network filtering methods are implemented and three different software packages are used to investigate the efficiency of the image orientation and modeling of the statue in the filtered (reduced) image networks. Internal and external validation results prove that minimal image networks can provide highly accurate records and efficiency in terms of visualization, completeness, processing time (>60% reduction) and the final accuracy of 1 mm.

  11. Minimal Camera Networks for 3D Image Based Modeling of Cultural Heritage Objects

    PubMed Central

    Alsadik, Bashar; Gerke, Markus; Vosselman, George; Daham, Afrah; Jasim, Luma

    2014-01-01

    3D modeling of cultural heritage objects like artifacts, statues and buildings is nowadays an important tool for virtual museums, preservation and restoration. In this paper, we introduce a method to automatically design a minimal imaging network for the 3D modeling of cultural heritage objects. This becomes important for reducing the image capture time and processing when documenting large and complex sites. Moreover, such a minimal camera network design is desirable for imaging non-digitally documented artifacts in museums and other archeological sites to avoid disturbing the visitors for a long time and/or moving delicate precious objects to complete the documentation task. The developed method is tested on the Iraqi famous statue “Lamassu”. Lamassu is a human-headed winged bull of over 4.25 m in height from the era of Ashurnasirpal II (883–859 BC). Close-range photogrammetry is used for the 3D modeling task where a dense ordered imaging network of 45 high resolution images were captured around Lamassu with an object sample distance of 1 mm. These images constitute a dense network and the aim of our study was to apply our method to reduce the number of images for the 3D modeling and at the same time preserve pre-defined point accuracy. Temporary control points were fixed evenly on the body of Lamassu and measured by using a total station for the external validation and scaling purpose. Two network filtering methods are implemented and three different software packages are used to investigate the efficiency of the image orientation and modeling of the statue in the filtered (reduced) image networks. Internal and external validation results prove that minimal image networks can provide highly accurate records and efficiency in terms of visualization, completeness, processing time (>60% reduction) and the final accuracy of 1 mm. PMID:24670718

  12. PCA-based spatially adaptive denoising of CFA images for single-sensor digital cameras.

    PubMed

    Zheng, Lei; Lukac, Rastislav; Wu, Xiaolin; Zhang, David

    2009-04-01

    Single-sensor digital color cameras use a process called color demosiacking to produce full color images from the data captured by a color filter array (CAF). The quality of demosiacked images is degraded due to the sensor noise introduced during the image acquisition process. The conventional solution to combating CFA sensor noise is demosiacking first, followed by a separate denoising processing. This strategy will generate many noise-caused color artifacts in the demosiacking process, which are hard to remove in the denoising process. Few denoising schemes that work directly on the CFA images have been presented because of the difficulties arisen from the red, green and blue interlaced mosaic pattern, yet a well-designed "denoising first and demosiacking later" scheme can have advantages such as less noise-caused color artifacts and cost-effective implementation. This paper presents a principle component analysis (PCA)-based spatially-adaptive denoising algorithm, which works directly on the CFA data using a supporting window to analyze the local image statistics. By exploiting the spatial and spectral correlations existing in the CFA image, the proposed method can effectively suppress noise while preserving color edges and details. Experiments using both simulated and real CFA images indicate that the proposed scheme outperforms many existing approaches, including those sophisticated demosiacking and denoising schemes, in terms of both objective measurement and visual evaluation.

  13. Intravital imaging of cardiac function at the single-cell level.

    PubMed

    Aguirre, Aaron D; Vinegoni, Claudio; Sebas, Matt; Weissleder, Ralph

    2014-08-05

    Knowledge of cardiomyocyte biology is limited by the lack of methods to interrogate single-cell physiology in vivo. Here we show that contracting myocytes can indeed be imaged with optical microscopy at high temporal and spatial resolution in the beating murine heart, allowing visualization of individual sarcomeres and measurement of the single cardiomyocyte contractile cycle. Collectively, this has been enabled by efficient tissue stabilization, a prospective real-time cardiac gating approach, an image processing algorithm for motion-artifact-free imaging throughout the cardiac cycle, and a fluorescent membrane staining protocol. Quantification of cardiomyocyte contractile function in vivo opens many possibilities for investigating myocardial disease and therapeutic intervention at the cellular level.

  14. TU-A-12A-02: Novel Lung Ventilation Imaging with Single Energy CT After Single Inhalation of Xenon: Comparison with SPECT Ventilation Images

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Negahdar, M; Yamamoto, T; Shultz, D

    Purpose: We propose a novel lung functional imaging method to determine the spatial distribution of xenon (Xe) gas in a single inhalation as a measure of regional ventilation. We compare Xe-CT ventilation to single-photon emission CT (SPECT) ventilation, which is the current clinical reference. Regional lung ventilation information may be useful for the diagnosis and monitoring of pulmonary diseases such as COPD, radiotherapy planning, and assessing the progression of toxicity after radiation therapy. Methods: In an IRB-approved clinical study, Xe-CT and SPECT ventilation scans were acquired for three patients including one patient with severe emphysema and two lung cancer patientsmore » treated with radiotherapy. For Xe- CT, we acquired two breath-hold single energy CT images of the entire lung with inspiration of 100% O2 and a mixture of 70% Xe and 30% O2, respectively. A video biofeedback system was used to achieve reproducible breath-holds. We used deformable image registration to align the breathhold images with each other to accurately subtract them, producing a map of the distribution of Xe as a surrogate of lung ventilation. We divided each lung into twelve parts and correlated the Hounsfield unit (HU) enhancement at each part with the SPECT ventilation count of the corresponding part of the lung. Results: The mean of the Pearson linear correlation coefficient values between the Xe-CT and ventilation SPECT count for all three patients were 0.62 (p<0.01). The Xe-CT image had a higher resolution than SPECT, and did not show central airway deposition artifacts that were present in the SPECT image. Conclusion: We developed a rapid, safe, clinically practical, and potentially widely accessible method for regional lung functional imaging. We demonstrated strong correlations between the Xe-CT ventilation image and SPECT ventilation image as the clinical reference. This ongoing study will investigate more patients to confirm this finding.« less

  15. Age effect in generating mental images of buildings but not common objects.

    PubMed

    Piccardi, L; Nori, R; Palermo, L; Guariglia, C; Giusberti, F

    2015-08-18

    Imagining a familiar environment is different from imagining an environmental map and clinical evidence demonstrated the existence of double dissociations in brain-damaged patients due to the contents of mental images. Here, we assessed a large sample of young and old participants by considering their ability to generate different kinds of mental images, namely, buildings or common objects. As buildings are environmental stimuli that have an important role in human navigation, we expected that elderly participants would have greater difficulty in generating images of buildings than common objects. We found that young and older participants differed in generating both buildings and common objects. For young participants there were no differences between buildings and common objects, but older participants found easier to generate common objects than buildings. Buildings are a special type of visual stimuli because in urban environments they are commonly used as landmarks for navigational purposes. Considering that topographical orientation is one of the abilities mostly affected in normal and pathological aging, the present data throw some light on the impaired processes underlying human navigation. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  16. Early Light Imaging for Biomedical Applications

    DTIC Science & Technology

    2000-07-01

    such as prostate cancer, breast cancer, cervical cancer, glaucoma , macular degeneration, macular endema, and atherosclerosis plaques. Understanding of...harmonic- generation cross-correlation time gating", Opt. Lett., 16 1019-1021 (1991) 17. K. Yoo, Z. Zang, S. Ahmed , R. Alfano, "Imaging objects hidden

  17. The VLT LBG Redshift Survey - III. The clustering and dynamics of Lyman-break galaxies at z ˜ 3

    NASA Astrophysics Data System (ADS)

    Bielby, R.; Hill, M. D.; Shanks, T.; Crighton, N. H. M.; Infante, L.; Bornancini, C. G.; Francke, H.; Héraudeau, P.; Lambas, D. G.; Metcalfe, N.; Minniti, D.; Padilla, N.; Theuns, T.; Tummuangpak, P.; Weilbacher, P.

    2013-03-01

    We present a catalogue of 2135 galaxy redshifts from the VLT LBG Redshift Survey (VLRS), a spectroscopic survey of z ≈ 3 galaxies in wide fields centred on background quasi-stellar objects. We have used deep optical imaging to select galaxies via the Lyman-break technique. Spectroscopy of the Lyman-break galaxies (LBGs) was then made using the Very Large Telescope (VLT) Visible Multi-Object Spectrograph (VIMOS) instrument, giving a mean redshift of z = 2.79. We analyse the clustering properties of the VLRS sample and also of the VLRS sample combined with the smaller area Keck-based survey of Steidel et al. From the semiprojected correlation function, wp(σ), for the VLRS and combined surveys, we find that the results are well fit with a single power-law model, with clustering scale lengths of r0 = 3.46 ± 0.41 and 3.83 ± 0.24 h-1 Mpc, respectively. We note that the corresponding combined ξ(r) slope is flatter than for local galaxies at γ = 1.5-1.6 rather than γ = 1.8. This flat slope is confirmed by the z-space correlation function, ξ(s), and in the range 10 < s < 100 h-1 Mpc the VLRS shows an ≈2.5σ excess over the Λ cold dark matter (ΛCDM) linear prediction. This excess may be consistent with recent evidence for non-Gaussianity in clustering results at z ≈ 1. We then analyse the LBG z-space distortions using the 2D correlation function, ξ(σ, π), finding for the combined sample a large-scale infall parameter of β = 0.38 ± 0.19 and a velocity dispersion of sqrt{< w_z^2rangle }=420^{+140}_{-160} km s^{-1}. Based on our measured β, we are able to determine the gravitational growth rate, finding a value of f(z = 3) = 0.99 ± 0.50 (or fσ8 = 0.26 ± 0.13), which is the highest redshift measurement of the growth rate via galaxy clustering and is consistent with ΛCDM. Finally, we constrain the mean halo mass for the LBG population, finding that the VLRS and combined sample suggest mean halo masses of log(MDM/M⊙) = 11.57 ± 0.15 and 11.73 ± 0

  18. Geographic Object-Based Image Analysis – Towards a new paradigm

    PubMed Central

    Blaschke, Thomas; Hay, Geoffrey J.; Kelly, Maggi; Lang, Stefan; Hofmann, Peter; Addink, Elisabeth; Queiroz Feitosa, Raul; van der Meer, Freek; van der Werff, Harald; van Coillie, Frieke; Tiede, Dirk

    2014-01-01

    The amount of scientific literature on (Geographic) Object-based Image Analysis – GEOBIA has been and still is sharply increasing. These approaches to analysing imagery have antecedents in earlier research on image segmentation and use GIS-like spatial analysis within classification and feature extraction approaches. This article investigates these development and its implications and asks whether or not this is a new paradigm in remote sensing and Geographic Information Science (GIScience). We first discuss several limitations of prevailing per-pixel methods when applied to high resolution images. Then we explore the paradigm concept developed by Kuhn (1962) and discuss whether GEOBIA can be regarded as a paradigm according to this definition. We crystallize core concepts of GEOBIA, including the role of objects, of ontologies and the multiplicity of scales and we discuss how these conceptual developments support important methods in remote sensing such as change detection and accuracy assessment. The ramifications of the different theoretical foundations between the ‘per-pixel paradigm’ and GEOBIA are analysed, as are some of the challenges along this path from pixels, to objects, to geo-intelligence. Based on several paradigm indications as defined by Kuhn and based on an analysis of peer-reviewed scientific literature we conclude that GEOBIA is a new and evolving paradigm. PMID:24623958

  19. Refocusing-range and image-quality enhanced optical reconstruction of 3-D objects from integral images using a principal periodic δ-function array

    NASA Astrophysics Data System (ADS)

    Ai, Lingyu; Kim, Eun-Soo

    2018-03-01

    We propose a method for refocusing-range and image-quality enhanced optical reconstruction of three-dimensional (3-D) objects from integral images only by using a 3 × 3 periodic δ-function array (PDFA), which is called a principal PDFA (P-PDFA). By directly convolving the elemental image array (EIA) captured from 3-D objects with the P-PDFAs whose spatial periods correspond to each object's depth, a set of spatially-filtered EIAs (SF-EIAs) are extracted, and from which 3-D objects can be reconstructed to be refocused on their real depth. convolutional operations are performed directly on each of the minimum 3 × 3 EIs of the picked-up EIA, the capturing and refocused-depth ranges of 3-D objects can be greatly enhanced, as well as 3-D objects much improved in image quality can be reconstructed without any preprocessing operations. Through ray-optical analysis and optical experiments with actual 3-D objects, the feasibility of the proposed method has been confirmed.

  20. Single-random-phase holographic encryption of images

    NASA Astrophysics Data System (ADS)

    Tsang, P. W. M.

    2017-02-01

    In this paper, a method is proposed for encrypting an optical image onto a phase-only hologram, utilizing a single random phase mask as the private encryption key. The encryption process can be divided into 3 stages. First the source image to be encrypted is scaled in size, and pasted onto an arbitrary position in a larger global image. The remaining areas of the global image that are not occupied by the source image could be filled with randomly generated contents. As such, the global image as a whole is very different from the source image, but at the same time the visual quality of the source image is preserved. Second, a digital Fresnel hologram is generated from the new image, and converted into a phase-only hologram based on bi-directional error diffusion. In the final stage, a fixed random phase mask is added to the phase-only hologram as the private encryption key. In the decryption process, the global image together with the source image it contained, can be reconstructed from the phase-only hologram if it is overlaid with the correct decryption key. The proposed method is highly resistant to different forms of Plain-Text-Attacks, which are commonly used to deduce the encryption key in existing holographic encryption process. In addition, both the encryption and the decryption processes are simple and easy to implement.