Science.gov

Sample records for zavisimostyi ot massy

  1. Overview of OT

    SciTech Connect

    Pate, R.; Dooley, R.B.

    1995-01-01

    At the last International Fossil Cycle Chemistry Conference in June 1991, a report was given on the Electric Power Research Institute (EPRI) sponsored project to study and transfer technology on the use of oxygenated feedwater treatment (OT). The report basically summarized the excellent results of employing OT worldwide, and updated the project activities which included development of an EPRI guidance document for implementation of OT in US utilities. Since the OT conversions of Georgia Power`s Wansley Unit No. 1 and Ohio Edison`s Sammis Unit 5 in November 1991, over 30 once-through units have subsequently been converted. In 1994, the firstmore » US drum unit was converted. The results have been outstanding with very large reductions of feedwater corrosion products (usually Fe<1 ppb at the economizer inlet) as a result of the more oxidizing environment (>+120 mV) and the change of the surface oxide layer from magnetite to ferric oxide hydrate (FeOOH) throughout the feedwater system. The benefits accrue because FeOOH blocks the pores of original Fe{sub 3}O{sub 4} reducing the transport of oxygen and iron ions through the layer. These surface layers of FeOOH also have a much lower solubility in flowing feedwater. This paper will provide an overview of the two supercritical/subcritical unit feedwater treatment technologies, (OT & AVT). In addition, the results of the two EPRI OT demonstration units (Wansley 1, Georgia Power Co. & Sammis 5, Ohio Edison) will be presented.« less

  2. Other Transaction (OT) Authority

    DTIC Science & Technology

    2008-11-25

    definition may be found in a Public Contract Law Journal article: “For purposes of this article, when the term ‘commercial’ is used, it encompasses all...the Bayh-Dole Act: Keeping the Federal Government on the Cutting Edge,” Public Contract Law Journal, vol. 30, no. 2 (winter 2001), p. 226.) The...Government? Should They Be?” Public Contract Law Journal, vol. 33, no. 1 (fall 2003), p. 26. The benefit of OT authority is that it “... allows

  3. Master-Saao new OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Kornilov, V.; Tiurina, N.; Kuznetsov, A.; Vladimirov, V.; Vlasenko, D.; Chazov, V.; Gress, O.; Zimnukhov, D.; Kuvshinov, D.; Shumkov, V.; Gorelkina, V.; Pogrosheva, T.; Gabovich, A.

    2018-04-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 58m 35.32s -35d 40m 42.2s on 2018-04-12.08618 UT. The OT unfiltered magnitude is (mlim=19.0).

  4. Samuel P. Massie Chair of Excellence In Environmental Disciplines: Hampton University 1994-2010 Year Report

    SciTech Connect

    Adeyiga, Adeyinka A.

    2014-12-17

    The establishment of the DOE-EM Dr. Samuel P. Massie Chair of Excellence provides an excellent opportunity for Hampton University to be involved in key environmental issues in the 21 st Century. The main areas of focus are on: 1. Coal gasification with respect to pollution prevention and reduction. 2. Solid waste treatment through bioremediation technology and 3. Industrial wastewater treatment Synthesizing ion catalysts suitable for use in slurry bubble column reaction was carried out. Construction of an autoclave continuous stirred tank reactor has been completed. At the initial stage of the development of this program, work was conducted in themore » area of formic acid recovery from waste streams, which yielded useful results. We also succeeded in the removal of priority metal ions such as cadmium, chromium, copper, lead, mercury, nickel, silver, thallium, zinc, etc., from industrial and municipal wastewater by using natural wastes. The process uses tree leaves to adsorb the metal ions in the wastewater. The ultimate goal is to develop inexpensive, highly available, effective metal ion adsorbents from natural wastes as an alternative to existing commercial adsorbents, and also to explain the possible adsorption mechanism that is taking place. This technology uses natural wastes to eliminate other wastes. Obviously, there are several advantages: (1) the negative impact on environment is eliminated, (2) the complicated regeneration step is not needed, and (3) the procedure saves money and energy. Twelve different types of leaves have been tested with lead, zinc, and nickel. The study mechanism showed that the leaf tannin is an active ingredient in the adsorption of metal ions. The ion-exchange mechanism controlled the adsorption process.« less

  5. Superhump Period of MASTER OT

    NASA Astrophysics Data System (ADS)

    Garnavich, Peter; McClelland, Colin

    2013-02-01

    We observed the optical transient MASTER OT J065608.28+744455.2 (ATEL #4783) with the Vatican Advanced Technology Telescope (VATT) and VATT4K CCD camera. V-band imaging began at 2013 Feb. 5.15 (UT) and continued for 3.3 hours with a time resolution of 22 seconds.

  6. MASTER: high amplitude OT and OT during Fermi inspection

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Rebolo, R.; Serra-Ricart, M.; Podesta, R.; Levato, H.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Shumkov, V.; Gress, O.; Pogrosheva, T.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gorbunov, I.; Krylov, A.; Lopez, C.; Podesta, F.; Saffe, C.; Gabovich, A.

    2017-06-01

    During trigger (short GRB)/ (GRB_TIME: 17/06/04 14:28:05.09 UT, GRB_RA,Dec(2000):22h 41m 46s,+40d 40' 12", GRB_ERROR: 4.10 [deg radius, statistical only] )inspection MASTER-Kislovodsk auto-detection system detected optical transient at (RA, Dec) = 23h 01m 48.65s +41d 19m 04.2s on 2017-06-04.97120 UT. The OT unfiltered magnitude is 19.2m (limit 19.3m).

  7. Time comparison via OTS-2

    NASA Technical Reports Server (NTRS)

    Dejong, G.; Kaarls, R.; Kirchner, D.; Ressler, H.

    1982-01-01

    The time comparisons carried out via OTS-2 between the Technical University Graz (Austria) and the Van Swinden Laboratory Delft (Netherlands) are discussed. The method is based on the use of the synchronization pulse in the TV-frame of the daily evening broadcasting of a French TV-program to Northern Africa. Corrections, as a consequence of changes in the position of the satellite coordinates are applied weekly after reception of satellite coordinates. A description of the method is given as well as some of the particular techniques used in both the participating laboratories. Preliminary results are presented.

  8. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... parties to lawsuits in which the OTS is not a party. (2) Unpublished OTS information includes records created or obtained in connection with the OTS's performance of its responsibilities, such as records... affiliates, and records compiled in connection with the OTS's enforcement responsibilities. Unpublished OTS...

  9. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... parties to lawsuits in which the OTS is not a party. (2) Unpublished OTS information includes records created or obtained in connection with the OTS's performance of its responsibilities, such as records... affiliates, and records compiled in connection with the OTS's enforcement responsibilities. Unpublished OTS...

  10. MASTER-OAFA: new OT outburst

    NASA Astrophysics Data System (ADS)

    Savinov, D.; Shumkov, V.; Lipunov, V.; Podesta, R.; Levato, H.; Kornilov, V.; Gorbovskoy, E.; Tiurina, N.; Gorbunov, I.; Balanutsa, P.; Kuznetsov, A.; Ishmuhametova, Yu.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Pogrosheva, T.; Zimnukhov, D.; Lopez, C.; Podesta, F.; Saffe, C.; Senik, V.; Gabovich, A.; Kuvshinov, D.

    2018-04-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 09h 09m 00.56s +04d 49m 04.1s on 2018-04-16.11132 UT. The OT unfiltered magnitude is 17.2m (mlim=19.3m).

  11. MASTER: OT in M31 direction

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Kornilov, V.; Gorbovskoy, E.; Tiurina, N.; Gorbunov, I.; Senik, V.; Chazov, V.; Kuznetsov, A.; Vladimirov, V.; Vlasenko, D.; Zimnukhov, D.; Tlatov, O. Gress A.; Shumkov, A. Gabovich V.; Pogrosheva, T.

    2018-06-01

    MASTER-Kislovodsk auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 00h 43m 55.89s +41d 32m 09.9s on 2018-06-20.98169 UT. The OT unfiltered magnitude is 19.0m (limit 19.6m).

  12. OT calibration and service maintenance manual.

    DOT National Transportation Integrated Search

    2012-01-01

    The machine conditions, as well as the values at the calibration and control parameters, may determine the quality of each test results obtained. In order to keep consistency and accuracy, the conditions, performance and measurements of an OT must be...

  13. An evolving computational platform for biological mass spectrometry: workflows, statistics and data mining with MASSyPup64.

    PubMed

    Winkler, Robert

    2015-01-01

    In biological mass spectrometry, crude instrumental data need to be converted into meaningful theoretical models. Several data processing and data evaluation steps are required to come to the final results. These operations are often difficult to reproduce, because of too specific computing platforms. This effect, known as 'workflow decay', can be diminished by using a standardized informatic infrastructure. Thus, we compiled an integrated platform, which contains ready-to-use tools and workflows for mass spectrometry data analysis. Apart from general unit operations, such as peak picking and identification of proteins and metabolites, we put a strong emphasis on the statistical validation of results and Data Mining. MASSyPup64 includes e.g., the OpenMS/TOPPAS framework, the Trans-Proteomic-Pipeline programs, the ProteoWizard tools, X!Tandem, Comet and SpiderMass. The statistical computing language R is installed with packages for MS data analyses, such as XCMS/metaXCMS and MetabR. The R package Rattle provides a user-friendly access to multiple Data Mining methods. Further, we added the non-conventional spreadsheet program teapot for editing large data sets and a command line tool for transposing large matrices. Individual programs, console commands and modules can be integrated using the Workflow Management System (WMS) taverna. We explain the useful combination of the tools by practical examples: (1) A workflow for protein identification and validation, with subsequent Association Analysis of peptides, (2) Cluster analysis and Data Mining in targeted Metabolomics, and (3) Raw data processing, Data Mining and identification of metabolites in untargeted Metabolomics. Association Analyses reveal relationships between variables across different sample sets. We present its application for finding co-occurring peptides, which can be used for target proteomics, the discovery of alternative biomarkers and protein-protein interactions. Data Mining derived models

  14. MASTER: OT detection during Fermi trigger inspection

    NASA Astrophysics Data System (ADS)

    Popova, E.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Ivanov, K.; Potter, S.; Gabovich, A.

    2016-11-01

    During inspection of Fermi trigger 501261070 ( (Ra,Dec)=47.190,-47.210; GRB_ERROR_radius=3.27deg, GRB_TIME=2016/11/19 15:11:06.40UT http://gcn.gsfc.nasa.gov/other/501261070.fermi ) MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 03h 22m 52.70s -48d 29m 10.9s on 2016-11-19 21:17:17.878UT with unfiltered m_OT=17.8 (mlim=19.7).

  15. First direct comparison of clinical outcomes between European and Asian cohorts in transcatheter aortic valve implantation: the Massy study group vs. the PREVAIL JAPAN trial.

    PubMed

    Watanabe, Yusuke; Hayashida, Kentaro; Takayama, Morimasa; Mitsudo, Kazuaki; Nanto, Shinsuke; Takanashi, Shuichiro; Komiya, Tatsuhiko; Kuratani, Toru; Tobaru, Tetsuya; Goto, Tsuyoshi; Lefèvre, Thierry; Sawa, Yoshiki; Morice, Marie-Claude

    2015-02-01

    The efficacy and safety of transcatheter aortic valve implantation (TAVI) in Asian populations were unknown. The purpose of this study was to compare directly the clinical outcomes of the first Japanese trial and a European single-center experience after TAVI. Between April 2010 and October 2011, 64 patients were included in the PREVAIL JAPAN multicenter trial which was set up to evaluate the safety and efficacy of the Edwards SAPIEN XT™ (Edwards Lifesciences, Irvine, CA, USA) in high-risk Japanese patients with severe aortic stenosis. Between March 2010 and January 2012, 237 consecutive patients treated with TAVI using the Edwards SAPIEN XT™ prosthesis at Institut Cardiovasculaire Paris Sud were prospectively included in the Massy cohort. We compared the clinical outcomes of these two cohorts. Patients were of similar age (83.4±6.6 years vs. 84.5±6.1 years, p=0.25), but logistic EuroSCORE was higher in the Massy cohort (20.2±11.7% vs. 15.6±8.0%, p<0.01). Body surface area was smaller in the PREVAIL JAPAN cohort (1.41±0.14m(2) vs. 1.72±0.18m(2); p<0.01) as was the annulus diameter (20.4±1.46mm vs. 22.0±1.84mm, p<0.01). The transfemoral approach was used in 57.8% in the Japanese cohort vs. 51.5% in the Massy cohort. Device success was similar (89.1% vs. 94.1%, p=0.21, respectively), as well as 30-day and 6-month survival rates (92.2% vs. 90.7% and 89.1% vs. 83.1%, p=0.71 and p=0.25, respectively). The incidence of major vascular complications was not significantly different between the two groups (9.4% vs. 5.9%, p=0.23, respectively). A higher post-procedural mean pressure gradient was observed in the PREVAIL JAPAN cohort (12.7±11.4mmHg vs. 10.1±3.6mmHg, p=0.01), but satisfactory improvement in 6-month functional status was obtained in both cohorts (76.5% vs. 77.2%, p=0.91). Clinical outcomes after TAVI in the patients included in the PREVAIL JAPAN trial were acceptable and as safe as that of a single-center European cohort. Copyright © 2014 Japanese

  16. High amplutude bright OT detected by MASTER-OAFA

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Lipunov, V.; Podesta, R.; Levato, H.; Pogrosheva, T.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Gorbunov, I.; Krylov, A.; Lopez, C.; Podesta, F.

    2018-05-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net",Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 17h 08m 14.73s -19d 05m 58.3s on 2018-05-12.37420 UT.The OT unfiltered magnitude is 15.9m (mlim=18.2m).The OT is seen in 4 images.

  17. Organic field-effect transistor with octadecyltrichlorosilane (OTS) self-assembled monolayers on gate oxide: effect of OTS quality

    NASA Astrophysics Data System (ADS)

    Devynck, M.; Tardy, P.; Wantz, G.; Nicolas, Y.; Hirsch, L.

    2011-12-01

    The effect of OTS (octadecyltrichlorosilane) Self-Assembled Monolayer (SAM) grafted on SiO2 gate dielectric of pentacene-based OFETs (organic field-effect transistors) is investigated. A significant improvement of the charge mobility (μ), up to 0.74 cm2/V s, is reached thanks to OTS treatment. However, in spite of improved performances, several drawbacks, such as an increase in mobility dispersion, substantial hysteresis in IDS-VG characteristics and high threshold voltages (VT), are observed. Changing solvent and deposition method turns out to have no significant effect on the mobility dispersion. A more accurate approach on the evolution of the mobility and the threshold voltage dispersion with OTS storage time highlights the effect of the OTS solution aging. Even if no difference is evidenced in the surface energy and roughness of the OTS layer, electrical characteristics exhibit considerable deterioration with OTS solution storage time. Using an "aged" OTS solution, opened under air, kept under argon and distilled before use, results in an increase of the IDS-VG hysteresis as well as in VT and in mobility dispersion. In comparison, fresh-OTS-based OFETs present a very low hysteresis, a threshold voltage close to 0 and a much lower mobility dispersion. It is demonstrated that aged OTS solutions contain impurities that are not removed by distillation process, which leads to a less densely packed layer causing interfacial charge traps thus deteriorated performances.

  18. MASTER-Kislovodsk: new OT, ampl>6m

    NASA Astrophysics Data System (ADS)

    Vladimirov, V.; Lipunov, V.; Gorbovskoy, E.; Tiurina, N.; Kornilov, V.; Balanutsa, P.; Kuznetsov, A.; Chazov, V.; Gress, O.; Vlasenko, D.; Pogrosheva, T.; Zimnukhov, D.; Senik, V.; Kuvshinov, D.; Rebolo, R.; Serra-Ricart, M.; Gabovich, A.

    2018-05-01

    MASTER-Kislovodsk auto-detection system discovered OT source at (RA, Dec) = 03h 19m 57.28s +77d 11m 22.8s on 2018-04-29.83595 UT during Fermi trigger inspection (but this OT is not connected with GRB).

  19. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 6 2012-01-01 2012-01-01 false Release of unpublished OTS information. 510.5 Section 510.5 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY MISCELLANEOUS ORGANIZATIONAL REGULATIONS § 510.5 Release of unpublished OTS information. (a) Scope. (1) This section applies to...

  20. 75 FR 51169 - OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-18

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision [Docket ID: OTS-2010-0026] OTS Minority Depository Institutions Advisory Committee AGENCY: Department of the Treasury, Office of Thrift Supervision...: Deirdre A. Foley, Designated Federal Official, (202) 906-5750, Office of Thrift Supervision, 1700 G Street...

  1. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false How do I notify OTS? 555.310 Section 555.310 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ELECTRONIC OPERATIONS Requirements Applicable to All Savings Associations § 555.310 How do I notify OTS? (a) Notice requirement. You...

  2. MASTER Net: OT in M31 direction and new CV

    NASA Astrophysics Data System (ADS)

    Shumkov, V.; Pogrosheva, T.; Lipunov, V.; Lopez, R. Rebolo; Serra-Ricart, M.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Zimnukhov, D.; Vladimirov, V.; Vlasenko, D.; Kuvshinov, D.

    2018-01-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 03h 00m 57.16s -66d 25m 23.1s on 2018-01-11.89176 UT. The OT unfiltered magnitude is 19.2m (mlim=20.1).

  3. The Rotation Period and Magnetic Field of the T Dwarf 2MASSI J1047539+212423 Measured from Periodic Radio Bursts

    NASA Astrophysics Data System (ADS)

    Williams, P. K. G.; Berger, E.

    2015-08-01

    Periodic radio bursts from very low mass stars and brown dwarfs simultaneously probe their magnetic and rotational properties. The brown dwarf 2MASSI J1047539+212423 (2M 1047+21) is currently the only T dwarf (T6.5) detected at radio wavelengths. Previous observations of this source with the Arecibo observatory revealed intermittent, 100%-polarized radio pulses similar to those detected from other brown dwarfs, but were unable to constrain a pulse periodicity; previous Very Large Array (VLA) observations detected quiescent emission a factor of ∼100 times fainter than the Arecibo pulses but no additional events. Here we present 14 hr of VLA observations of this object that reveal a series of pulses at ∼6 GHz with highly variable profiles, showing that the pulsing behavior evolves on time scales that are both long and short compared to the rotation period. We measure a periodicity of ∼1.77 hr and identify it with the rotation period. This is just the sixth rotation period measurement in a late T dwarf, and the first obtained in the radio. We detect a pulse at 10 GHz as well, suggesting that the magnetic field strength of 2 M 1047+21 reaches at least 3.6 kG. Although this object is the coolest and most rapidly rotating radio-detected brown dwarf to date, its properties appear continuous with those of other such objects, suggesting that the generation of strong magnetic fields and radio emission may continue to even cooler objects. Further studies of this kind will help to clarify the relationships between mass, age, rotation, and magnetic activity at and beyond the end of the main sequence, where both theories and observational data are currently scarce.

  4. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... their official capacities. Examples of unpublished information include: (i) Information in the memory of... must be sent to the OTS at 1700 G Street NW., Washington, DC 20552, to the attention of the Corporate...

  5. Disruptive Ideas for IT/OT Security in Energy Systems

    SciTech Connect

    Martin, Maurice

    Presentation on disruptive ideas for IT and OT cybersecurity in energy systems, presented by Power Systems Engineering Center Researcher Maurice Martin. This presentation was prepared for the Florida Institute for Cybersecurity Research Conference on March 1, 2018.

  6. 12 CFR 500.10 - The OTS or The Office.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false The OTS or The Office. 500.10 Section 500.10 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY AGENCY ORGANIZATION AND FUNCTIONS General Organization § 500.10 The OTS or The Office. The Office of Thrift Supervision (referred to as “OTS” or “Office”) is an office of the...

  7. OT promotes closer interpersonal distance among highly empathic individuals.

    PubMed

    Perry, Anat; Mankuta, David; Shamay-Tsoory, Simone G

    2015-01-01

    The space between people, or 'interpersonal distance', creates and defines the dynamics of social interactions and is a salient cue signaling responsiveness and feeling comfortable. This distance is implicit yet clearly felt, especially if someone stands closer or farther away than expected. Increasing evidence suggests that Oxytocin (OT) serves as a social hormone in humans, and that one of its roles may be to alter the perceptual salience of social cues. Considering that empathic ability may shape the way individuals process social stimuli, we predicted that OT will differentially affect preferred interpersonal distance depending on individual differences in empathy. Participants took part in two interpersonal distance experiments: In the first, they had to stop a (computer visualized) protagonist when feeling most comfortable; in the second, they were asked to choose the room in which they would later discuss intimate topics with another. Both experiments revealed an interaction between the effect of OT and empathy level. Among highly empathic individuals, OT promoted the choice of closer interpersonal distances. Yet, OT had an opposite effect on individuals with low empathic traits. We conclude that the enhancement of social cues following OT administration may have opposite effects on individuals with different empathic abilities. © The Author (2014). Published by Oxford University Press. For Permissions, please email: journals.permissions@oup.com.

  8. A new assessment method of outdoor tobacco smoke (OTS) exposure

    NASA Astrophysics Data System (ADS)

    Cho, Hyeri; Lee, Kiyoung

    2014-04-01

    Outdoor tobacco smoke (OTS) is concerned due to potential health effects. An assessment method of OTS exposure is needed to determine effects of OTS and validate outdoor smoking policies. The objective of this study was to develop a new method to assess OTS exposure. This study was conducted at 100 bus stops including 50 centerline bus stops and 50 roadside bus stops in Seoul, Korea. Using real-time aerosol monitor, PM2.5 was measured for 30 min at each bus stop in two seasons. ‘Peak analysis' method was developed to assess short term PM2.5 exposure by OTS. The 30-min average PM2.5 exposure at each bus stop was associated with season and bus stop location but not smoking activity. The PM2.5 peak occurrence rate by the peak analysis method was significantly associated with season, bus stop location, observed smoking occurrence, and the number of buses servicing a route. The PM2.5 peak concentration was significantly associated with season, smoking occurrence, and the number of buses servicing a route. When a smoker was standing still at the bus stop, magnitude of peak concentrations were significantly higher than when the smoker walking-through the bus stop. People were exposed to high short-term PM2.5 peak levels at bus stops, and the magnitude of peak concentrations were highest when a smoker was located close to the monitor. The magnitude of peak concentration was a good indicator helped distinguish nearby OTS exposure. Further research using ‘peak analysis' is needed to measure smoking-related exposure to PM2.5 in other outdoor locations.

  9. 8. Photocopy of a drawing (original in the Collection ot ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Photocopy of a drawing (original in the Collection ot the PL&C, Shelf 128, Drawing 1766) PLAN OF PART OF THE MERRIMACK RIVER (PLAN OF DAM), FEBRUARY 1833 - Lowell Canal System, Pawtucket Dam, Merrimack River,above Pawtucket Falls, Lowell, Middlesex County, MA

  10. Counterpart of MASTER OT J084140.94+722732.6

    NASA Astrophysics Data System (ADS)

    Nesci, Roberto

    2016-02-01

    Following the ATel #8725 by Balanutsa et al. about MASTER OT J084140.94+722732.6, I made aperture photometry on all the Palomar survey plates (12) available from the MAST archive to get an indication of the star color.

  11. Recent optical activity of the blazar OT 355

    NASA Astrophysics Data System (ADS)

    Bachev, R.; Kurtenkov, A.; Nikolov, Y.; Spassov, B.; Boeva, S.; Latev, G.; Dimitrova, R. V. Munoz

    2017-06-01

    The Flat Spectrum Radio Quasar OT 355 (also known as 7C 173240.70+385949.00, z=0.975) was typically observed to be in the optical between 16th and 21th magnitude (CRTS, http://nesssi.cacr.caltech.edu/catalina/20011332/113321380764100137p.html).

  12. Genome sequence of the algicidal bacterium Kordia algicida OT-1.

    PubMed

    Lee, Hyun Sook; Kang, Sung Gyun; Kwon, Kae Kyoung; Lee, Jung-Hyun; Kim, Sang-Jin

    2011-08-01

    Kordia algicida OT-1 is an algicidal bacterium against the bloom-forming microalgae. The genome sequence of K. algicida revealed a number of interesting features, including the degradation of macromolecules, the biosynthesis of carotenoid pigment and secondary metabolites, and the capacity for gliding motility, which might facilitate the understanding of algicidal mechanisms.

  13. 12 CFR 502.50 - What fees does OTS charge?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What fees does OTS charge? 502.50 Section 502.50 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES... consolidated with a savings association on the Consolidated Statement of Condition of the Thrift Financial...

  14. Teaching the Moving Child: OT Insights That Will Transform Your K-3 Classroom

    ERIC Educational Resources Information Center

    Berkey, Sybil M.

    2009-01-01

    Because sensorimotor and environmental factors have a profound effect on children's learning, every teacher should know how to weave strategies from occupational therapy (OT) into their everyday instruction. This is the guidebook K-3 teachers need to "think like an OT"--and form effective partnerships with OTs in their schools--so all students can…

  15. 12 CFR 555.300 - Must I inform OTS before I use electronic means or facilities?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Must I inform OTS before I use electronic means... I inform OTS before I use electronic means or facilities? (a) General. A savings association (“you”) are not required to inform OTS before you use electronic means or facilities, except as provided in...

  16. 12 CFR 563b.115 - How will OTS review my business plan?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How will OTS review my business plan? 563b.115... business plan? (a) OTS will review your business plan to determine that it demonstrates a safe and sound... will be determinative. OTS will review every case on its merits. (b) You must file your business plan...

  17. 12 CFR 563b.675 - What conditions will OTS impose on an approval?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What conditions will OTS impose on an approval... Supervisory Conversion Application § 563b.675 What conditions will OTS impose on an approval? (a) OTS will... conditions and restrictions on you (before and after the conversion), your acquiror, controlling parties, or...

  18. 12 CFR 516.10 - How does OTS compute time periods under this part?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... APPLICATION PROCESSING PROCEDURES § 516.10 How does OTS compute time periods under this part? In computing time periods under this part, OTS does not include the day of the act or event that commences the time... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS compute time periods under this...

  19. On the light-curve of OT/GRB970508

    NASA Astrophysics Data System (ADS)

    Pedersen, H.; Jaunsen, A. O.; Østensen, R.; Grav, T.; Wold, M.; Lacy, M.; Kristen, H.; Broeils, A.; Castro-Tirado, A. J.; Gorosabel, J.; Rodriguez Espinosa, J. M.; Perez, A. M.; Näslund, M.; Fransson, C.; Andersen, M. I.; Wolf, C.; Fockenbrock, R.; Piro, L.; Feroci, M.; Costa, E.; Nicastro, L.; Palazzi, E.; Frontera, F.; Monaldi, L.; Heise, J.; Hjorth, J.

    1998-05-01

    Using the Nordic Optical Telescope, La Palma, Spain, optical studies of OT/GRB970508 were initiated 3 hours 5 minutes after the high energy event (This appears to be sooner than achieved elsewhere, for any optical transient). The OT was clearly detected in the earliest images. The last observation was done August 13-15, i.e. 95-97 days after the event. We have combined the NOT R and unfiltered data with similar photometry from elsewhere. We conclude that the observed source brightness cannot easily be modeled by theories predicting a monotonic rise to maximum. Also the post-maximum power-law decay, albeit predicted by several models, is no longer valid at the moment of our last observation. The contribution of a constant source, mR=25.5 is indicated, but this does not show up in the image profile.

  20. Optimization of OT-MACH Filter Generation for Target Recognition

    NASA Technical Reports Server (NTRS)

    Johnson, Oliver C.; Edens, Weston; Lu, Thomas T.; Chao, Tien-Hsin

    2009-01-01

    An automatic Optimum Trade-off Maximum Average Correlation Height (OT-MACH) filter generator for use in a gray-scale optical correlator (GOC) has been developed for improved target detection at JPL. While the OT-MACH filter has been shown to be an optimal filter for target detection, actually solving for the optimum is too computationally intensive for multiple targets. Instead, an adaptive step gradient descent method was tested to iteratively optimize the three OT-MACH parameters, alpha, beta, and gamma. The feedback for the gradient descent method was a composite of the performance measures, correlation peak height and peak to side lobe ratio. The automated method generated and tested multiple filters in order to approach the optimal filter quicker and more reliably than the current manual method. Initial usage and testing has shown preliminary success at finding an approximation of the optimal filter, in terms of alpha, beta, gamma values. This corresponded to a substantial improvement in detection performance where the true positive rate increased for the same average false positives per image.

  1. Selective and non-selective OT receptor agonists induce different locomotor behaviors in male rats via central OT receptors and peripheral V1a receptors.

    PubMed

    Wolfe, Monica; Wisniewska, Halina; Tariga, Hiroe; Ibanez, Gerardo; Collins, James C; Wisniewski, Kazimierz; Qi, Steve; Srinivasan, Karthik; Hargrove, Diane; Lindstrom, Beatriz Fioravanti

    2018-05-21

    Oxytocin (OT) continues to inspire much research due to its diverse physiological effects. While the best-understood actions of OT are uterine contraction and milk ejection, OT is also implicated in maternal and bonding behaviors, and potentially in CNS disorders such as autism, schizophrenia, and pain. The dissection of the mechanism of action of OT is complicated by the fact that this peptide activates not only its cognate receptor but also vasopressin type 1a (V1a) receptors. In this study, we evaluated OT and a selective OT receptor (OTR) agonist, FE 204409, in an automated assay that measures rat locomotor activity. The results showed: 1) Subcutaneous (sc) administration of OT decreased locomotor behavior (distance traveled, stereotypy, and rearing). This effect was reversed by a V1a receptor (V1aR) antagonist ([Pmp1,Tyr(ME)2]AVP, sc), suggesting that OT acts through peripheral V1aR to inhibit locomotor activity. 2) A selective OTR agonist (FE 204409, sc) increased stereotypy. This effect was reversed by an OTR antagonist dosed icv, suggesting a central OTR site of action. Our findings identify distinct behavioral effects for OT and the selective agonist FE 204409, adding to the growing body of evidence that the V1aR mediates many effects attributed to OT and that peptides administered systemically at supra-physiological doses may activate receptors in the brain. Our studies further emphasize the importance of utilizing selective agonists and antagonists to assess therapeutic indications. Copyright © 2018 Elsevier Ltd. All rights reserved.

  2. The ALMA OT in early science: supporting multiple customers

    NASA Astrophysics Data System (ADS)

    Bridger, Alan; Williams, Stewart; McLay, Stewart; Yatagai, Hiroshi; Schilling, Marcus; Biggs, Andrew; Tobar, Rodrigo; Warmels, Rein H.

    2012-09-01

    The ALMA Observatory is currently operating 'Early Science' observing. The Cycle0 and Cycle1 Calls for Proposals are part of this Early Science, and in both the ALMA Observing Tool plays a crucial role. This paper describes how the ALMA OT tackles the problem of making millimeter/sub-millimeter interferometry accessible to the wider community, while allowing "experts" the power and flexibility they need. We will also describe our approach to the challenges of supporting multiple customers, and explore the lessons learnt from the Early Science experiences. Finally we look ahead to the challenges presented by future observing cycles.

  3. MASTER-SAAO: contradictory SN and flaring OT

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Buckley, D.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Kornilov, V.; Gress, O.; Pogrosheva, T.; Shumkov, V.; Vladimirov, V.; Vlasenko, D.; Kuvshinov, D.; Gabovich, A.

    2017-07-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 03h 42m 50.70s , -01d 52m 28s.7 on 2017-07-01.1753UT with unfiltered (6 images), that contradicts to Ia type detected in ATEL #10240 , ATEL #10225 for ATLAS17dcl ( http://www.supernova.thistlethwaites.com/sn2017/sndate.html ). It is in 8.4"W,20"S of PGC135685 with Btc=15.13, Vgsr=2773 http://leda.univ-lyon1.fr/ledacat.cgi?PGC135685 Spectral observations are required.

  4. Radiation Susceptibility Assessment of Off the Shelf (OTS) Hardware

    NASA Technical Reports Server (NTRS)

    Culpepper, William X.; Nicholson, Leonard L. (Technical Monitor)

    2000-01-01

    The reduction in budgets, shortening of schedules and necessity of flying near state of the art technology have forced projects and designers to utilize not only modern, non-space rated EEE parts but also OTS boards, subassemblies and systems. New instrumentation, communications, portable computers and navigation systems for the International Space Station, Space Shuttle, and Crew Return Vehicle are examples of the realization of this paradigm change at the Johnson Space Center. Because of this change, there has been a shift in the radiation assessment methodology from individual part testing using low energy heavy ions to board and box level testing using high-energy particle beams. Highlights of several years of board and system level testing are presented along with lessons learned, present areas of concern, insights into test costs, and future challenges.

  5. 12 CFR 550.80 - How do I obtain OTS approval?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false How do I obtain OTS approval? 550.80 Section 550.80 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Obtaining Fiduciary Powers § 550.80 How do I obtain OTS approval? You must file an...

  6. 12 CFR 550.80 - How do I obtain OTS approval?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How do I obtain OTS approval? 550.80 Section 550.80 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Obtaining Fiduciary Powers § 550.80 How do I obtain OTS approval? You must file an...

  7. OT discovered by MASTER during Fermi Trigger Num 430645968 follow up observations follow up observations

    NASA Astrophysics Data System (ADS)

    Tiurina, N.; Balanutsa, P.; Denisenko, D.; Lipunov, V.; Gorbovskoy, E.; Kornilov, V.; Chazov, V.; Kuznetsov, A.; Vladimirov, V.; Yecheistov, V.; Shumkov, V.; Ivanov, K.; Yazev, S.; Budnev, N.; Konstantinov, E.; Chuvalaev, O.; Poleshchuk, V.; Gress, O.; Parkhomenko, A.; Tlatov, A.; Dormidontov, D.; Senik, V.; Yurkov, V.; Sergienko, Y.; Varda, D.; Sinyakov, E.; Gabovich, A.; Krushinsky, V.; Zalozhnih, I.; Popov, A.; Bourdanov, A.; Podvorotny, P.; Shurpakov, S.; Levato, H.; Saffe, C.; Mallamaci, C.; Lopez, C.; Podest, F.

    2014-08-01

    MASTER-Tunka auto-detection system during follow-up Fermi Trigger 430645968 observations (GCN 16745) discovered OT source at: MASTER-Tunka auto-detection system discovered MASTER OT J230448.20+324534.1 at (RA, Dec) = 23h 04m 48.20s +32d 45m 34.1s on 2014-08-25.62245 UT.

  8. 12 CFR 516.250 - Will OTS require me to publish a new public notice?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Will OTS require me to publish a new public... publish a new public notice? (a) If your application was subject to a publication requirement, OTS may require you to publish a new public notice of your application if: (1) You submitted a revision to the...

  9. 12 CFR 563.146 - Will the OTS permit my capital distribution?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... under 12 U.S.C. 1831o(d)(1)(B). (b) Your proposed capital distribution raises safety or soundness... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Will the OTS permit my capital distribution... SAVINGS ASSOCIATIONS-OPERATIONS Capital Distributions § 563.146 Will the OTS permit my capital...

  10. 12 CFR 550.120 - What action will the OTS take on my application?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false What action will the OTS take on my application? 550.120 Section 550.120 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Obtaining Fiduciary Powers § 550.120 What action will the OTS take...

  11. 12 CFR 550.120 - What action will the OTS take on my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What action will the OTS take on my application? 550.120 Section 550.120 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Obtaining Fiduciary Powers § 550.120 What action will the OTS take...

  12. 75 FR 63895 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-18

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision [Docket ID: OTS-2010-0028] Open Meeting of... of Thrift Supervision. ACTION: Notice of meeting. SUMMARY: The OTS Minority Depository Institutions... the Office of Thrift Supervision, 1700 G Street, NW., Washington, DC, beginning at 9 a.m. Eastern Time...

  13. 75 FR 61572 - Open Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-05

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision [Docket ID OTS-2010-0030] Open Meeting of... Thrift Supervision. ACTION: Notice of meeting. SUMMARY: The OTS Mutual Savings Associations Advisory....treas.gov ; or Mail: to Charlotte Bahin, Designated Federal Official, Office of Thrift Supervision, 1700...

  14. 75 FR 38188 - Closed Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-01

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision [Docket ID OTS-2010-0021] Closed Meeting... of Thrift Supervision. ACTION: Notice of Closed Meeting. SUMMARY: The OTS Mutual Savings Associations... Supervision, 1700 G Street, NW., Washington, DC 20552. The public is invited to submit written statements to...

  15. 75 FR 76524 - Closed Meeting of the OTS Mutual Savings Association Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-08

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision [Docket ID: OTS-2010-0034] Closed Meeting of the OTS Mutual Savings Association Advisory Committee AGENCY: Office of Thrift Supervision... Supervision, 1700 G Street, NW., Washington, DC 20552. The public is invited to submit written statements to...

  16. 76 FR 7630 - Open Meeting of the OTS Minority Depository Institutions Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-10

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision [Docket ID: OTS-2011-0003] Open Meeting of... of Thrift Supervision. ACTION: Notice of meeting. SUMMARY: The OTS Minority Depository Institutions... Office of Thrift Supervision, 1700 G Street, NW., Washington, DC, beginning at 9 a.m. [[Page 7631...

  17. 12 CFR 516.170 - When will OTS conduct a meeting on an application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... application, or otherwise determines that a meeting will benefit the decision-making process. OTS may limit... inform the applicant and all commenters requesting a meeting of its decision to grant or deny a meeting request, or of its decision to conduct a meeting on its own initiative. (c) If OTS decides to conduct a...

  18. A Scoping Review of the Tensions in OT-Teacher Collaborations

    ERIC Educational Resources Information Center

    Wintle, James; Krupa, Terry; Cramm, Heidi; DeLuca, Christopher

    2017-01-01

    Teachers must meet the educational needs of diverse students. Occupational therapists (OTs) assist some of these students through the provision of school-based occupational therapy (SBOT). However, there can be challenges to collaboration between OTs and teachers, and this can engender difficulty in achieving desired results. This scoping review…

  19. 12 CFR 502.60 - When will OTS adjust, add, waive, or eliminate a fee?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When will OTS adjust, add, waive, or eliminate a fee? 502.60 Section 502.60 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES Fees § 502.60 When will OTS adjust, add, waive, or eliminate a fee? Under...

  20. 12 CFR 502.55 - Where can I find OTS's fee schedule?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Where can I find OTS's fee schedule? 502.55 Section 502.55 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND... fees in a Thrift Bulletin. OTS will publish these fees at least 30 days before they are effective. ...

  1. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you exercise...

  2. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you exercise...

  3. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you exercise...

  4. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you exercise...

  5. 12 CFR 550.70 - Must I obtain OTS approval or file a notice before I exercise fiduciary powers?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... before I exercise fiduciary powers? 550.70 Section 550.70 Banks and Banking OFFICE OF THRIFT SUPERVISION... I obtain OTS approval or file a notice before I exercise fiduciary powers? You should refer to the following chart to determine if you must obtain OTS approval or file a notice with OTS before you exercise...

  6. 75 FR 181 - Application for Conversion From: (a) OTS-Regulated, State-Chartered Savings Association to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-04

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision Application for Conversion From: (a) OTS...: Application for Conversion from: (1) OTS- Regulated, State-Chartered Savings Association to Federal Saving... whether it meets applicable eligibility requirements for conversion and complies with applicable OTS...

  7. First report on the occurrence of Theileria sp. OT3 in China.

    PubMed

    Tian, Zhancheng; Liu, Guangyuan; Yin, Hong; Xie, Junren; Wang, Suyan; Yuan, Xiaosong; Wang, Fangfang; Luo, Jin

    2014-04-01

    Theileria sp. OT3 was firstly detected and identified from clinically healthy sheep in Xinjiang Uygur Autonomous Region of China (XUAR) through comparing the complete 18S rDNA gene sequences available in GenBank database and the phylogenetic status based on the internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene by the methods of a partitioned multi-locus analysis in BEAST and Maximum likelihood analysis in PhyML. Moreover, the findings were confirmed by the species-specific PCR for Theileria sp. OT3 and the prevalence of Theileria sp. OT3 was 14.9% in the north of XUAR. This study is the first report on the occurrence of Theileria sp. OT3 in China. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  8. 12 CFR 563b.200 - What actions may OTS take on my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... record under part 563e of this chapter and your business plan to determine how you will serve the... that you will serve. (2) OTS may deny your application if your business plan does not demonstrate that...

  9. OT2_smalhotr_3: Herschel Extreme Lensing Line Observations (HELLO)

    NASA Astrophysics Data System (ADS)

    Malhotra, S.

    2011-09-01

    We request 59.8 hours of Herschel time to observe 20 normal star-forming galaxies in the [CII] 158 micron and [OI] 63 micron lines. These galaxies lie at high redshift (1OT1, 49 high redshift IR luminous galaxies were approved for spectroscopy, but only two so-called normal galaxies were included. This is an imbalance that should be corrected, to balance Herschel's legacy.

  10. Effects of Hypergravity Exposure on Plasma Oxytocin (OT) Concentrations in Pregnant and Lactating Rat Dams

    NASA Technical Reports Server (NTRS)

    Baer, Lisa A.; Wade, Charles E.; Plaut, Karen; Ronca, April E.; Dalton, Bonnie (Technical Monitor)

    2002-01-01

    From pregnancy to weaning there is a progressive elevation of plasma oxytocin (OT) levels associated with nursing activity, irrespective of litter size. In the present study, we analyzed the effects of continuous 1.5G, 1.75G and 2.0G hypergravity exposure on OT plasma concentration in prepartum (Gestation Day 20) (G20) and lactating (Postnatal day) (P10) rat dams. For this study, litter size was controlled with a yoking procedure established in our lab where individual control litters were yoked-matched to individual hypergravity litters. We reviewed all data at hypergravity irrespective of gravitational level and compared the values with the controls in both G20 (HG, n=15;SC, n=9) and P10 (HG, n=21;SC, n=16). Results showed that over time, we did observe the expected OT increase in both groups. In G20 dams, measurement of OT concentrations showed no significance. However, at P10, measurements of OT concentrations suggest a reduction of about 20% compared to established controls in our laboratory, 0.9+/-0.09 ng/ml for the controls and 0.7+/-0.06 ng/ml for centrifuged animals (p<0.02). These data suggest that exposure to centrifugation may reduce OT levels during lactation. When these plasma samples were obtained, the dams were removed from the litters, and values were not adjusted for the size of the litters. The reduction in OT with centrifugation may reflect a decrease in nursing activity or a decreased responsiveness of the mammary hypothalamic axis. In addition, we have analyzed data on plasma prolactin concentrations and mammary gland development, which may give additional insight to the results of our OT measurements.

  11. Oxytocin (OT) and arginine-vasopressin (AVP) act on OT receptors and not AVP V1a receptors to enhance social recognition in adult Syrian hamsters (Mesocricetus auratus).

    PubMed

    Song, Zhimin; Larkin, Tony E; Malley, Maureen O'; Albers, H Elliott

    2016-05-01

    Social recognition is a fundamental requirement for all forms of social relationships. A majority of studies investigating the neural mechanisms underlying social recognition in rodents have investigated relatively neutral social stimuli such as juveniles or ovariectomized females over short time intervals (e.g., 2h). The present study developed a new testing model to study social recognition among adult males using a potent social stimulus. Flank gland odors are used extensively in social communication in Syrian hamsters and convey important information such as dominance status. We found that the recognition of flank gland odors after a 3min exposure lasted for at least 24h, substantially longer than the recognition of other social cues in rats and mice. Intracerebroventricular injections of OT and AVP prolonged the recognition of flank gland odor for up to 48h. Selective OTR but not V1aR agonists, mimicked these enhancing effects of OT and AVP. Similarly, selective OTR but not V1aR antagonists blocked recognition of the odors after 20min. In contrast, the recognition of non-social stimuli was not blocked by either the OTR or the V1aR antagonists. Our findings suggest both OT and AVP enhance social recognition via acting on OTRs and not V1aRs and that the recognition enhancing effects of OT and AVP are limited to social stimuli. Copyright © 2016 Elsevier Inc. All rights reserved.

  12. The potential for oxytocin (OT) to prevent breast cancer: a hypothesis.

    PubMed

    Murrell, T G

    1995-08-01

    This hypothesis proposes that carcinogens in the breast are generated by the action of superoxide free radicals released when acinal gland distension, under the influence of unopposed prolactin, causes microvessel ischaemia. Inadequate nipple care in the at-risk years leads to ductal obstruction preventing the elimination of carcinogens from the breast. The regular production of oxytocin (OT) from nipple stimulation would cause contraction of the myoepithelial cells, relieving acinal gland distension and aiding the active elimination of carcinogenic fluid from the breast. Mechanical breast pump stimulation causes an increase in plasma OT levels in the luteal but not in the follicular phase of the menstrual cycle. OT production upon nipple stimulation in the luteal phase of premenopausal, non-lactating women may be protective against the high rates of mitotic breast cell division noted at this time via the potential to block the effect of oestrogen. The epidemiology of breast cancer suggests that lengthy lactation time is beneficial. Sexual activity in nulliparous women also protects and OT levels have been shown to rise with orgasm in women and in men. OT systems in the brain are intricately linked to oestrogen and progesterone levels, and it is possible that these hormones may modify the OT secretory response both centrally and through an effect on the sensitivity of the breast. OT production with nipple care and in sex and lactation, and the reduction in cycling ovarian hormones that occurs with pregnancy, may all be important preventative factors in the development of breast cancer both pre- and post-menopausally.

  13. VHF Direction Finder (VDF) Operational Test and Evaluation (OT&E) integration and OT&E Operational Test Logs and Data

    DTIC Science & Technology

    1994-05-01

    4. 2 + 30 1 120 K1. Dl f o’fZ. 5 $ Time 143 q Data___________ Plot.,- s A "’ke-., v CO Pi.ot -----.-.. n•_ ze g .,nL,.,khav ,.-VW ý61I;, 9- 0 ; $ 4...Saemario 0 5 . Two Airaraft. apraimately 45 minutes. D1Fre ; 12T. Z.-t._--__ Aircraft a1 iiuot-Mg A~uomy-ss 3nq1?O" lLock~v*otf time 2 13 9a Aircraft 02...1 a b ul a z %# U,’t YIV M" us~N b Ct u U, ý at nOt P SI am N% EA . 0 40 on N n -q P"I n n I - 44~~K 5 ~ - - N N mul 3 UA ’YM ly! oni - 4 b eq a

  14. Software OT&E Guidelines. Volume 3. Software Maintainability Evaluator’s Handbook

    DTIC Science & Technology

    1980-04-01

    SOFTWARE OT&E " 1 GUIDELINES . VOLUME III SOFTWARE MAINTAINABILITY EVALUATOR’S HANDBOOK APRIL 1980 AIR FORCE TEST AND EVALUATION CENTER KIRTLAND AIR...FORCE BASE NEW MEXICO 87117 C-, -j AfTECP 800-3 AF’r...........3 ...... UNCLASSIFIED SECURITY CLASSIFICATION OF THIS PAGE (When D.. Entered) RE:PORT...c -. 5 TY!aJ0. PERIOD COVERED SOFTWARE OT& . GUIDELINES, Volume III .of five). -1 softare-R.aintainability Evaluator’s P-IEFnook’ 4ina. i 1980

  15. MASTER OT J072007.30+451611.6: Cataclysmic Variable with an Extreme Hot Spot

    NASA Astrophysics Data System (ADS)

    Denisenko, D.

    2018-05-01

    MASTER team has reported an unusual object MASTER OT J072007.30+451611.6 in ATel #11620 (T. Pogrosheva et al.). The object was observed at 17.0-17.2m during 9 minutes from 22:15:26 to 22:24:30 UT on 2018-04-26, but was below the detection limit (18.8m) 45 minutes before the first positive observation and 43 minutes after the last one. As noted by S. Otero in AAVSO VSX entry, MASTER OT J072007.30+451611.6 has an X-ray counterpart XMMSL2 J072007.4+451615.

  16. A global outer-rise/outer-trench-slope (OR/OTS) earthquake study

    NASA Astrophysics Data System (ADS)

    Wartman, J. M.; Kita, S.; Kirby, S. H.; Choy, G. L.

    2009-12-01

    Using improved seismic, bathymetric, satellite gravity and other geophysical data, we investigated the seismicity patterns and focal mechanisms of earthquakes in oceanic lithosphere off the trenches of the world that are large enough to be well recorded at teleseismic distances. A number of prominent trends are apparent, some of which have been previously recognized based on more limited data [1], and some of which are largely new [2-5]: (1) The largest events and the highest seismicity rates tend to occur where Mesozoic incoming plates are subducting at high rates (e.g., those in the western Pacific and the Banda segment of Indonesia). The largest events are predominantly shallow normal faulting (SNF) earthquakes. Less common are reverse-faulting (RF) events that tend to be deeper and to be present along with SNF events where nearby seamounts, seamount chains and other volcanic features are subducting [Seno and Yamanaka, 1996]. Blooms of SNF OR/OTS events usually occur just after and seaward of great interplate thrust (IPT) earthquakes but are far less common after smaller IPT events. (2) Plates subducting at slow rates (<20 mm/a) often show sparse OR/OTS seismicity. It is unclear if such low activity is a long-term feature of these systems or is a consequence of the long return times of great IPT earthquakes (e.g., the sparse OR/OTS seismicity before the 26 December 2004 M9.2 Sumatra earthquake and many subsequent OR/OTS events). (3) OR/OTS shocks are generally sparse or absent where incoming plates are very young (<20 Ma) (e.g., Cascadia, southern Mexico, Nankai, and South Shetlands). (4) Subducting plates of intermediate age (20 to about 65 Ma) display a diversity of focal mechanisms and seismicity patterns. In the Philippines, NE Indonesia, and Melanesia, bands of reverse faulting events occur at or near the trench and SNF earthquakes are restricted to OR/OTS sites further from the trench. (5) Clustering of OR/OTS events of all types commonly occurs where

  17. 75 FR 10560 - Application for Conversion From: (a) OTS-Regulated, State-Chartered Savings Association to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision Application for Conversion From: (a) OTS... Proposal: Application for Conversion from: (1) OTS- Regulated, State-Chartered Savings Association to... reviewed to determine whether it meets applicable eligibility requirements for conversion and complies with...

  18. 12 CFR 502.10 - How does OTS calculate the semi-annual assessment for savings associations?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... §§ 502.15 through 502.25 of this part. (b) OTS uses the September 30 Thrift Financial Report to determine amounts due at the January 31 assessment; and the March 31 Thrift Financial Report to determine amounts... your total assets as reported on Thrift Financial Reports filed with OTS. ...

  19. 12 CFR 502.28 - How does OTS determine the organizational form component for a savings and loan holding company?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... component for a savings and loan holding company? 502.28 Section 502.28 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ASSESSMENTS AND FEES Assessments Savings and Loan Holding Companies... savings and loan holding company that OTS regulates under section 10(l) of the HOLA. OTS will compute your...

  20. 12 CFR 550.560 - When may the OTS revoke my fiduciary powers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When may the OTS revoke my fiduciary powers? 550.560 Section 550.560 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Terminating Fiduciary Activities Revocation of Fiduciary Powers...

  1. 12 CFR 550.540 - When will the OTS terminate my fiduciary powers?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false When will the OTS terminate my fiduciary powers? 550.540 Section 550.540 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Terminating Fiduciary Activities Surrender of Fiduciary Powers...

  2. 12 CFR 550.580 - When may I conduct fiduciary activities without obtaining OTS approval?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false When may I conduct fiduciary activities without obtaining OTS approval? 550.580 Section 550.580 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Activities Exempt From This Part § 550.580...

  3. 12 CFR 550.540 - When will the OTS terminate my fiduciary powers?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false When will the OTS terminate my fiduciary powers? 550.540 Section 550.540 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Terminating Fiduciary Activities Surrender of Fiduciary Powers...

  4. 12 CFR 550.560 - When may the OTS revoke my fiduciary powers?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false When may the OTS revoke my fiduciary powers? 550.560 Section 550.560 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Terminating Fiduciary Activities Revocation of Fiduciary Powers...

  5. 78 FR 54401 - Removal of Transferred OTS Regulations Regarding Post-Employment Activities of Senior Examiners

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-04

    ... 336 and 390 RIN 3064-AD98 Removal of Transferred OTS Regulations Regarding Post-Employment Activities... Restrictions on Post-Employment Activities of Senior Examiners. This subpart was included in the regulations... restrictions for post-employment activities of senior examiners of all insured depository institutions for...

  6. 12 CFR 563.590 - When will the OTS waive the prior notice requirement?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... TREASURY SAVINGS ASSOCIATIONS-OPERATIONS Notice of Change of Director or Senior Executive Officer § 563.590... as a director or senior executive officer before filing a notice under this subpart if the OTS issues... before filing a notice under this subpart, if the individual was not nominated by management and the...

  7. 12 CFR 516.15 - Must I meet with OTS before I file my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... with OTS before filing your application. You must submit a draft business plan before this meeting. (2... you to submit a draft business plan or other relevant information before this meeting. (b) Contacting... pre-filing meeting or a draft business plan, the Regional Office will determine whether it will...

  8. Use of thermal cycling to reduce adhesion of OTS coated coated MEMS cantilevers

    NASA Astrophysics Data System (ADS)

    Ali, Shaikh M.; Phinney, Leslie M.

    2003-01-01

    °Microelectromechanical systems (MEMS) have enormous potential to contribute in diverse fields such as automotive, health care, aerospace, consumer products, and biotechnology, but successful commercial applications of MEMS are still small in number. Reliability of MEMS is a major impediment to the commercialization of laboratory prototypes. Due to the multitude of MEMS applications and the numerous processing and packaging steps, MEMS are exposed to a variety of environmental conditions, making the prediction of operational reliability difficult. In this paper, we investigate the effects of operating temperature on the in-use adhesive failure of electrostatically actuated MEMS microcantilevers coated with octadecyltrichlorosilane (OTS) films. The cantilevers are subjected to repeated temperature cycles and electrostatically actuated at temperatures between 25°C and 300°C in ambient air. The experimental results indicate that temperature cycling of the OTS coated cantilevers in air reduces the sticking probability of the microcantilevers. The sticking probability of OTS coated cantilevers was highest during heating, which decreased during cooling, and was lowest during reheating. Modifications to the OTS release method to increase its yield are also discussed.

  9. MELATONIN ENHANCES JUNCTIONAL TRANSFER IN NORMAL C3H/1OT1/2 CELLS

    EPA Science Inventory

    There is strong evidence that pineal melatonin is involved in controlling neoplastic processes. e have reported that physiological, but not pharmacological or subphysiological, concentrations of melatonin enhance intercellular communication in normal C3H/1OT1/2 fibroblasts. ap ju...

  10. Optimal Diphthongs: An OT Analysis of the Acquisition of Spanish Diphthongs

    ERIC Educational Resources Information Center

    Krause, Alice

    2013-01-01

    This dissertation investigates the acquisition of Spanish diphthongs by adult native speakers of English. The following research questions will be addressed: 1) How do adult native speakers of English pronounce sequences of two vowels in their L2 Spanish at different levels of acquisition? 2) Can OT learnability models, specifically the GLA,…

  11. Genome Sequence of the Algicidal Bacterium Kordia algicida OT-1 ▿

    PubMed Central

    Lee, Hyun Sook; Kang, Sung Gyun; Kwon, Kae Kyoung; Lee, Jung-Hyun; Kim, Sang-Jin

    2011-01-01

    Kordia algicida OT-1 is an algicidal bacterium against the bloom-forming microalgae. The genome sequence of K. algicida revealed a number of interesting features, including the degradation of macromolecules, the biosynthesis of carotenoid pigment and secondary metabolites, and the capacity for gliding motility, which might facilitate the understanding of algicidal mechanisms. PMID:21622754

  12. 12 CFR 585.120 - What factors will OTS consider in reviewing my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What factors will OTS consider in reviewing my application? 585.120 Section 585.120 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY PROHIBITED SERVICE AT SAVINGS AND LOAN HOLDING COMPANIES Exemptions § 585.120 What factors will...

  13. 12 CFR 555.300 - Must I inform OTS before I use electronic means or facilities?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false Must I inform OTS before I use electronic means or facilities? 555.300 Section 555.300 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY ELECTRONIC OPERATIONS Requirements Applicable to All Savings Associations § 555.300 Must...

  14. Spectroscopic Classification of MASTER OT J110707.62-052244.0 as a Type Ia Supernova

    NASA Astrophysics Data System (ADS)

    Zheng, W.; Kim, M.; Shivvers, I.; Yuk, H.; Filippenko, A. V.

    2015-11-01

    We report that inspection of a CCD spectrum (range 350-1050 nm) of MASTER OT J110707.62-052244.0 (ATel #8236), obtained on Nov. 11.57 UT with the Shane 3-m reflector (+ Kast spectrograph) at Lick Observatory, shows that the object is a normal Type Ia supernova roughly 1 week past maximum brightness.

  15. Spectroscopic Classification of MASTER OT J044212.20+230616.7

    NASA Astrophysics Data System (ADS)

    Shivvers, I.; Kelly, P. L.; Clubb, K. I.; Filippenko, A. V.

    2014-09-01

    We report that inspection of a CCD spectrum (range 330-1000 nm), obtained on September 22 UT with the 3-m Shane reflector (+ Kast) at Lick Observatory, shows that MASTER OT J044212.20+230616.7 (ATel #6484) is a Type IIn supernova.

  16. "Merging Yoga and Occupational Therapy (MY-OT): A feasibility and pilot study".

    PubMed

    Schmid, Arlene A; Puymbroeck, Marieke Van; Portz, Jennifer D; Atler, Karen E; Fruhauf, Christine A

    2016-10-01

    To examine the feasibility and benefits of the Merging Yoga and Occupational Therapy (MY-OT) intervention. This is the primary analysis of a non-controlled pretest-posttest pilot study to understand the feasibility and impact of MY-OT on balance, balance self-efficacy, and fall risk factor management in people with chronic stroke. University research laboratory. People with chronic stroke were included in the study if they: had sustained a fall or had fear of falling, were able to stand, and hand impaired balance and were at risk for falls (≤46 on the Berg Balance Scale (BBS)). Individuals completed an 8 week intervention that included 16 sessions of both yoga and group occupational therapy (OT). Yoga included physical postures, breathing exercises, and meditation. OT focused on post-stroke fall risk factor management. The BBS was used to assess balance, the Activities-specific Balance Confidence Scale (ABC) was used to measure balance self-efficacy. Five fall risk factor management scales were used. Overall, the intervention was considered feasible, as individuals were able to safely complete the intervention with little attrition and high attendance. Balance improved by 30% (p=0.002). Balance self-efficacy improved by 15% (p=0.034). Each of the five fall risk factor management scales improved, but only two significantly improved (Fall Prevention and Management Questionnaire, 29%, p=0.004 and Fall Prevention Strategy Survey, 42%, p=0.032). The results demonstrate that MY-OT is a potential intervention to improve multiple fall related outcomes for people with stroke. Therapists may consider these interventions for people with stroke, but additional research is warranted. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false How does OTS determine the risk/complexity...-Calculation of Assessments § 502.27 How does OTS determine the risk/complexity component for a savings and loan holding company? (a) OTS computes the risk/complexity component for responsible savings and loan...

  18. MASTER: bright OT discovered during Fermi trigger 512353690/GRB170328A inspection

    NASA Astrophysics Data System (ADS)

    Gorbovskoy, E.; Lipunov, V.; Buckley, D.; Rebolo, R.; Serra-Ricart, M.; Gress, O.; Tiurina, N.; Balanutsa, P.; Kornilov, V.; Vladimirov, V.

    2017-03-01

    MASTER-SAAO auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 45m 46.55s -35d 28m 47.6s on 2017-03-28.06645 UT during Fermi trigger 512353690(GRB170328A) inspection https://gcn.gsfc.nasa.gov/other/512353690.fermi (trigger time is 17/03/28 00:28:05.53UT).

  19. Software OT&E Guidelines. Volume 1. Software Test Manager’s Handbook

    DTIC Science & Technology

    1981-02-01

    on reverse side If neceeary and identify by block number) The Software OT&E Guidelines is a set of handbooks prepared by the Computer / Support Systems...is one of a set of handbooks prepared by the Computer /Support Systems Division of the Test and Evaluation Directorate, Air Force Test and Evaluation...15 E. Software Maintainability .. .. ........ ... 16 F. Standard Questionnaires. .. .. ....... .... 16 1. Operator- Computer Interface Evaluation

  20. MASTER OT J014638.27+041324.4 is a Young Type IIP Supernova

    NASA Astrophysics Data System (ADS)

    Zheng, W.; Kelly, P. L.; Clubb, K. I.; Filippenko, A. V.

    2013-12-01

    We report that a CCD spectrum (range 350-1000 nm) of MASTER OT J014638.27+041324.4 (Shurpakov et al., ATel #5630) was obtained on Dec 6.5 UT with the Shane 3-m reflector (+Kast spectrograph) at Lick Observatory. The spectrum shows a blue continuum and weak, broad hydrogen Balmer lines having P-Cyg profiles, indicating that the object is a young Type IIP supernova. Weak He I 587.6 nm is also present.

  1. Placental Expressions of CDKN1C and KCNQ1OT1 in Monozygotic Twins with Selective Intrauterine Growth Restriction.

    PubMed

    Gou, Chenyu; Liu, Xiangzhen; Shi, Xiaomei; Chai, Hanjing; He, Zhi-Ming; Huang, Xuan; Fang, Qun

    2017-10-01

    CDKN1C and KCNQ1OT1 are imprinted genes that might be potential regulators of placental development. This study investigated placental expressions of CDKN1C and KCNQ1OT1 in monozygotic twins with and without selective intrauterine growth restriction (sIUGR). Seventeen sIUGR and fifteen normal monozygotic(MZ) twin pairs were examined. Placental mRNA expressions of CDKN1C and KCNQ1OT1 were detected by real-time fluorescent quantitative PCR. CDKN1C protein expression was detected by immunohistochemical assay and Western-blotting. In the sIUGR group, smaller fetuses had a smaller share of the placenta, and CDKN1C protein expression was significantly increased while KCNQ1OT1 mRNA expression was significantly decreased. The CDKN1C/KCNQ1OT1 mRNA ratio was lower in the larger fetus than in the smaller fetus (p < .05). In the control group, CDKN1C protein expression showed no difference between larger and smaller fetuses, while KCNQ1OT1 mRNA expression was significantly lower in the larger fetus, and the CDKN1C/KCNQ1OT1 mRNA ratio was higher in the larger fetus than in the smaller fetus (p < .05). Our findings showed that pathogenesis of sIUGR may be related to the co-effect of the up-regulated protein expression of CDKN1C and down-regulated mRNA expression of KCNQ1OT1 in the placenta.

  2. Gene expression profiles of prohibitin in testes of Octopus tankahkeei (ot-phb) revealing its possible role during spermiogenesis.

    PubMed

    Mao, Hai-Tao; Wang, Da-Hui; Lan, Zhou; Zhou, Hong; Yang, Wan-Xi

    2012-05-01

    Prohibitin is essential for intracellular homeostasis and stabilization of mitochondrial respiratory chain complexes. To explore its functions during spermiogenesis of Octopus tankahkeei (O. tankahkeei), we have cloned and sequenced the cDNA of this mammalian PHB homologue (termed ot-PHB) from the testes of O. tankahkeei. The 1165 bp ot-phb cDNA contains a 100 bp 5' UTR, a 882 bp open reading frame and a 183 bp 3' UTR. The putative ot-PHB protein owns a transmembrane domain from 6 to 31 amino acid (aa) and a putative PHB domain from 26 to 178 aa. Protein alignment demonstrated that ot-PHB had 73.3, 73.6, 74.0, 75.1, and 45.4% identity with its homologues in Homo sapiens, Mus muculus, Danio rerio, Xenopus tropicalis and Trypanosoma brucei, respectively. Tissue distribution profile analysis revealed its presence in all the tissues examined. In situ hybridization in spermiogenic cells demonstrated that ot-phb was expressed moderately at the beginning of the spermiogenesis. The abundance of transcripts increased in intermediate spermatids and in drastically remodeling final spermatids. In mature spermatozoa, the residuary transcripts concentrated around the chondriosomal mantle where mitochondria assemble around. In summary, the expression of ot-phb during spermiogenesis implicates a potential function of this protein during mitochondrial ubiquitination. It is the first time to implicate the role of prohibitin in cephalopod spermiogenesis.

  3. Discordant KCNQ1OT1 imprinting in sets of monozygotic twins discordant for Beckwith-Wiedemann syndrome.

    PubMed

    Weksberg, Rosanna; Shuman, Cheryl; Caluseriu, Oana; Smith, Adam C; Fei, Yan-Ling; Nishikawa, Joy; Stockley, Tracy L; Best, Lyle; Chitayat, David; Olney, Ann; Ives, Elizabeth; Schneider, Adele; Bestor, Timothy H; Li, Madeline; Sadowski, Paul; Squire, Jeremy

    2002-05-15

    Beckwith-Wiedemann syndrome (BWS) presents with visceromegaly, macroglossia, tumor predisposition and other congenital abnormalities, and is usually associated with abnormalities of chromosome 11p15. A number of identical twin pairs, mostly female, have been reported to be discordant for BWS. We show here that the incidence of female monozygotic twins among patients with BWS is dramatically increased over that of the general population. A cluster of imprinted genes within 11p15 is thought to be coordinately regulated via the imprinted expression of KCNQ1OT1, which encodes an untranslated RNA. In skin fibroblasts from five monozygotic twin pairs discordant for BWS, each affected twin had an imprinting defect at KCNQ1OT1 on 11p15, whereas the unaffected twin did not. Five additional monozygotic twin pairs, for whom only blood was available, also displayed an imprinting defect at KCNQ1OT1. It is possible that discordance for BWS in MZ twins is due to unequal splitting of the inner cell mass during twinning, thereby causing differential maintenance of imprinting at KCNQ1OT1. Alternatively, we propose that KCNQ1OT1 is especially vulnerable to a loss of imprinting event, caused by a lack of maintenance DNA methylation at a critical stage of preimplantation development, and that this loss of imprinting predisposes to twinning as well as to discordance for BWS. These data underscore the importance of continued surveillance of children born following assisted reproductive technologies that impact the preimplantation embryo.

  4. Synthesis and structure of [Na11(OtBu)10(OH)]: 1H NMR shift of a hydroxide ion encapsulated in a 21-vertex alcoholate cage.

    PubMed

    Geier, Jens; Grützmacher, Hansjörg

    2003-12-07

    [Na11(OtBu)10(OH)], a hydroxide enclosing 21-vertex cage compound, was found to crystallize from mixtures of sodium tert.butanolate with sodium hydroxide. Its structure can be derived from the known (NaOtBu)6-hexaprismane by replacing one butanolate unit with OH- and capping the latter with five additional units of NaOtBu. The hydroxide shows a signal at -3.21 ppm in the 1H NMR spectrum.

  5. Spitzer Observations of the New Luminous Red Nova M85 OT2006-1

    NASA Astrophysics Data System (ADS)

    Rau, A.; Kulkarni, S. R.; Ofek, E. O.; Yan, L.

    2007-04-01

    M85 OT2006-1 is the latest and most brilliant addition to the small group of known luminous red novae (LRNe). An identifying characteristic of the previously detected events (M31 RV, V4332 Sgr, and V838 Mon) was a spectral redward evolution connected with an emerging infrared component following the optical decay. Here we report on the discovery of a similar feature in Keck NIRC and Spitzer photometry of M85 OT2006-1 6 months posteruption. We find that its 2.1-22 μm spectral energy distribution is best described by a blackbody with effective temperature Teff=950+/-150 K and bolometric luminosity L=2.9+0.4-0.5×105 Lsolar. Assuming spherical geometry, the blackbody effective radius, R=2.0+0.6-0.4×104 Rsolar, and corresponding expansion velocity, v=870+260-180 km s-1, are remarkably similar to the properties of M31 RV 70 days after its eruption. Furthermore, we propose a search strategy for LRNe in the local universe making use of the longevity of their infrared excess emission and discuss the expected number of events in the Spitzer Infrared Nearby Galaxies Survey.

  6. Solar-energy-system performance evaluation: Honeywell OTS 44, Ocmulgee, Georgia

    NASA Technical Reports Server (NTRS)

    Mathur, A. K.; Pederson, S.

    1982-01-01

    The operation and technical performance of the solar operational test site (OTS 44) are described, based on data collected between April, 1981 and August, 1981. The following topics are discussed: system description, performance assessment, operating energy, energy savings, system maintenance, and conclusions. The solar energy system at OTS 44 is a hydronic heating and cooling system consisting of 5040 square feet of liquid cooled flat plate collectors; a 4000 gallon thermal storage tank; one 25 ton capacity organic Rankine cycle engine assisted water chillers; a forced draft cooling tower; and associated piping, pumps, valves, controls and heat rejection equipment. The solar system has eight basic modes of operation and several combination modes for providing space conditioning and hot water to the building. Data monitored during the 4 months of the operational test period found that the solar system collected 285 MMBtu of thermal energy of the total incident solar energy of 1040 MMBtu and provided 210 MMBtu for cooling and 10 MMBtu for heating and hot water. The net electrical energy saving due to the solar system was approximately 2600 kWh(e), and fossil energy saving was about 20 million Btu (MMBtu).

  7. Accuracy of Conventional PCR Targeting the 16S rRNA Gene with the Ot-16sRF1 and Ot-16sRR1 Primers for Diagnosis of Scrub Typhus: a Case-Control Study

    PubMed Central

    Kim, Choon-Mee; Cho, Min Keun; Yun, Na-Ra; Kim, Seok Won; Jang, Sook Jin; Ahn, Young-Joon; Lim, Donghoon

    2015-01-01

    We retrospectively evaluated the accuracy of conventional PCR targeting the 16S rRNA gene (16S C-PCR) using the Ot-16sRF1/Ot-16sRR1 primers for diagnosing scrub typhus. The diagnosis of Orientia tsutsugamushi infection by 16S C-PCR presented an increased sensitivity of 87.0% and specificity of 100% compared with those obtained with other targets and is thus a simple and clinically useful method with good diagnostic accuracy. PMID:26491185

  8. Long noncoding RNA SOX2OT contributes to gastric cancer progression by sponging miR-194-5p from AKT2.

    PubMed

    Qu, Fei; Cao, Peilong

    2018-05-18

    Gastric cancer (GC) is a highly malignant cancer with poor prognosis. Long non-coding RNA (LncRNA) may play an important role in tumor progression. Our present study aimed to explore the effect of LncRNA SOX2OT on GC progression. We observed that SOX2OT was overexpressed in GC tissues and cell lines. Overexpressed SOX2OT promoted cell proliferation and metastasis of GC cells (SGC-7901, TMK-1) and the phosphorylation of AKT2 as well, while knockdown of SOX2OT reversed these effects. Besides that, miR-194-5p was predicted to be a target of SOX2OT and decreased expression of miR-194-5p was observed in GC tissues and cell lines. Overexpressed miR-194-5p counteracted the promoting role of SOX2OT on cell proliferation and invasion of GC cells. Moreover, AKT2 was predicted to be a target of miR-194-5p. The expression of AKT2 was negatively regulated by miR-194-5p while positively regulated by SOX2OT. Overexpressed AKT2 also promoted GC cell proliferation and invasion. Our in vitro experiments suggested that SOX2OT promoted cell proliferation and metastasis of GC cells via sponging miR-194-5p from AKT2. Finally, our in vivo experiments indicated that overexpressed SOX2OT promoted GC tumor growth and metastasis in nude mice. Taken together, our present study suggested that SOX2OT contributed to GC progression via sponging miR-194-5p from AKT2 both in vitro and in vivo. The SOX2OT-miR-194-5p-AKT2 axis may provide a new perspective for treatment of GC. Copyright © 2018. Published by Elsevier Inc.

  9. Trehalose-6-Phosphate-Mediated Toxicity Determines Essentiality of OtsB2 in Mycobacterium tuberculosis In Vitro and in Mice.

    PubMed

    Korte, Jan; Alber, Marina; Trujillo, Carolina M; Syson, Karl; Koliwer-Brandl, Hendrik; Deenen, René; Köhrer, Karl; DeJesus, Michael A; Hartman, Travis; Jacobs, William R; Bornemann, Stephen; Ioerger, Thomas R; Ehrt, Sabine; Kalscheuer, Rainer

    2016-12-01

    Trehalose biosynthesis is considered an attractive target for the development of antimicrobials against fungal, helminthic and bacterial pathogens including Mycobacterium tuberculosis. The most common biosynthetic route involves trehalose-6-phosphate (T6P) synthase OtsA and T6P phosphatase OtsB that generate trehalose from ADP/UDP-glucose and glucose-6-phosphate. In order to assess the drug target potential of T6P phosphatase, we generated a conditional mutant of M. tuberculosis allowing the regulated gene silencing of the T6P phosphatase gene otsB2. We found that otsB2 is essential for growth of M. tuberculosis in vitro as well as for the acute infection phase in mice following aerosol infection. By contrast, otsB2 is not essential for the chronic infection phase in mice, highlighting the substantial remodelling of trehalose metabolism during infection by M. tuberculosis. Blocking OtsB2 resulted in the accumulation of its substrate T6P, which appears to be toxic, leading to the self-poisoning of cells. Accordingly, blocking T6P production in a ΔotsA mutant abrogated otsB2 essentiality. T6P accumulation elicited a global upregulation of more than 800 genes, which might result from an increase in RNA stability implied by the enhanced neutralization of toxins exhibiting ribonuclease activity. Surprisingly, overlap with the stress response caused by the accumulation of another toxic sugar phosphate molecule, maltose-1-phosphate, was minimal. A genome-wide screen for synthetic lethal interactions with otsA identified numerous genes, revealing additional potential drug targets synergistic with OtsB2 suitable for combination therapies that would minimize the emergence of resistance to OtsB2 inhibitors.

  10. Trehalose-6-Phosphate-Mediated Toxicity Determines Essentiality of OtsB2 in Mycobacterium tuberculosis In Vitro and in Mice

    PubMed Central

    Koliwer-Brandl, Hendrik; Hartman, Travis; Jacobs, William R.; Ioerger, Thomas R.; Ehrt, Sabine

    2016-01-01

    Trehalose biosynthesis is considered an attractive target for the development of antimicrobials against fungal, helminthic and bacterial pathogens including Mycobacterium tuberculosis. The most common biosynthetic route involves trehalose-6-phosphate (T6P) synthase OtsA and T6P phosphatase OtsB that generate trehalose from ADP/UDP-glucose and glucose-6-phosphate. In order to assess the drug target potential of T6P phosphatase, we generated a conditional mutant of M. tuberculosis allowing the regulated gene silencing of the T6P phosphatase gene otsB2. We found that otsB2 is essential for growth of M. tuberculosis in vitro as well as for the acute infection phase in mice following aerosol infection. By contrast, otsB2 is not essential for the chronic infection phase in mice, highlighting the substantial remodelling of trehalose metabolism during infection by M. tuberculosis. Blocking OtsB2 resulted in the accumulation of its substrate T6P, which appears to be toxic, leading to the self-poisoning of cells. Accordingly, blocking T6P production in a ΔotsA mutant abrogated otsB2 essentiality. T6P accumulation elicited a global upregulation of more than 800 genes, which might result from an increase in RNA stability implied by the enhanced neutralization of toxins exhibiting ribonuclease activity. Surprisingly, overlap with the stress response caused by the accumulation of another toxic sugar phosphate molecule, maltose-1-phosphate, was minimal. A genome-wide screen for synthetic lethal interactions with otsA identified numerous genes, revealing additional potential drug targets synergistic with OtsB2 suitable for combination therapies that would minimize the emergence of resistance to OtsB2 inhibitors. PMID:27936238

  11. Entraining IDyOT: Timing in the Information Dynamics of Thinking

    PubMed Central

    Forth, Jamie; Agres, Kat; Purver, Matthew; Wiggins, Geraint A.

    2016-01-01

    We present a novel hypothetical account of entrainment in music and language, in context of the Information Dynamics of Thinking model, IDyOT. The extended model affords an alternative view of entrainment, and its companion term, pulse, from earlier accounts. The model is based on hierarchical, statistical prediction, modeling expectations of both what an event will be and when it will happen. As such, it constitutes a kind of predictive coding, with a particular novel hypothetical implementation. Here, we focus on the model's mechanism for predicting when a perceptual event will happen, given an existing sequence of past events, which may be musical or linguistic. We propose a range of tests to validate or falsify the model, at various different levels of abstraction, and argue that computational modeling in general, and this model in particular, can offer a means of providing limited but useful evidence for evolutionary hypotheses. PMID:27803682

  12. Definitize and Prepare Off-The-Shelf (OTS) Electronic Test Equipment (ETE) Specifications

    DTIC Science & Technology

    1978-07-01

    power consumption of the equipment shall be 30 watts. 3.5.2 Input power selection device. An input power selection device shall be provided for...36.2QCM( 14.25INIWX25.40r’’( 10 IM IHX 30 .48CM ( 121 MID 1U9.2,"M(43 IN1WX50.8CM( 20IN )HX57.15’"«I 22.5!N)D 36.8 3’"M(14.5IN IWX25.40rM( 10 IV...018 1437 3.63KG(aL8SI 2< 30 -a-MOD 11837 008 3350 8 .4KG’( 18.5L8S 1 315A 11837 008 3592 4.09KGt9L8S) OEFINITIZATITN "F OTS ETE

  13. OT2_nflagey_2: Capturing missing evolved stars in the Galactic plane

    NASA Astrophysics Data System (ADS)

    Flagey, N.

    2011-09-01

    We discovered more than 400 compact shells in the MIPSGAL 24 microns survey of the Galactic plane. About 15% of all these objects were already known as planetary nebulae, supernova remnants, Wolf-Rayet stars, and luminous blue variables. The unknown bubbles are expected to be envelopes of evolved stars that could account for the ``missing massive stars in the Galaxy. Indeed, recent spectroscopic follow-ups in the near-IR and mid-IR have revealed several dust-free planetary nebulae with very hot central white dwarf and significantly increased the number of WR and LBV candidates. Our OT1 Priority 1 proposal just provided us with a first observation in the PACS-SED B2A mode of one object, revealing only a strong [N II] 122 microns line. Without further spectral information, identification and modeling of the target are impossible. However, analysis of the PACS and SPIRE data from the HiGal survey has recently enabled us to measure much higher detection rates of the shells in the far-IR than with MIPS 70 microns. We are thus very confident that dust features and/or gas lines can be detected with the PACS and SPIRE spectrometers. Therefore, we request complementary PACS-SED B2B and SPIRE-FTS observations on our OT1 sample. The complete far-IR/submm spectrum of each target will allow its unequivocal identification thanks to comparison with spectra of known evolved stars from the MESS key program. We will also model with much detail the different phases of the envelopes, thanks to our expertise in circumstellar envelopes, dust models and photoionization codes.

  14. The Persistent Eruption of UGC 2773-OT: finally, a decade-long extragalactic Eta Carinae analogue

    NASA Astrophysics Data System (ADS)

    Smith, Nathan; Andrews, Jennifer E.; Mauerhan, Jon C.; Zheng, WeiKang; Filippenko, Alexei V.; Graham, Melissa L.; Milne, Peter

    2016-02-01

    While supernova (SN) impostors resemble the Great Eruption of η Carinae in the sense that their spectra show narrow H lines and they have typical peak absolute magnitudes of -13 to -14 mag, most extragalactic events observed so far are quite different from η Car in duration. Their bright phases typically last for ˜100 d or less, rather than persisting for several years. The transient object UGC 2773-OT (discovered in 2009) had a similar peak absolute magnitude to other SN impostors, but with a gradual 5-yr pre-discovery rise. In the ˜6 yr since discovery, it has faded very slowly (0.26 mag yr-1). Overall, we suggest that its decade-long eruption is so far the best-known analogue of η Car's 19th century eruption. We discuss extensive spectroscopy of the ongoing eruption. The spectra show interesting changes in velocity and line shape that we discuss in detail, including an asymmetric Hα emission line that we show is consistent with the ejection of a bipolar nebula that could be very much like the Homunculus of η Car. Moreover, changes in the line width, line profile, blue excess emission resembling that of Type IIn SNe, and the intensity of Hα suggest the presence of strong circumstellar interaction in the eruption at late times. This supports the hypothesis that the extended plateau of η Car's eruption may have been powered by shock interaction as well. One interesting difference compared to η Car, however, is that UGC 2773-OT so far does not exhibit the repeated brief spikes in luminosity that have been associated with binary periastron events.

  15. 12 CFR 502.27 - How does OTS determine the risk/complexity component for a savings and loan holding company?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... classifications and total consolidated holding company assets. OTS will establish these schedules in a Thrift Bulletin. (b) For the purposes of this section, the holding company risk/complexity classification is the most recent risk/complexity classification of which OTS notified the savings and loan holding company...

  16. 12 CFR 550.100 - What factors may the OTS consider in its review of my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false What factors may the OTS consider in its review of my application? 550.100 Section 550.100 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Obtaining Fiduciary Powers § 550.100 What...

  17. 12 CFR 550.100 - What factors may the OTS consider in its review of my application?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 5 2011-01-01 2011-01-01 false What factors may the OTS consider in its review of my application? 550.100 Section 550.100 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY FIDUCIARY POWERS OF SAVINGS ASSOCIATIONS Obtaining Fiduciary Powers § 550.100 What...

  18. 12 CFR 502.26 - How does OTS calculate the semi-annual assessment for savings and loan holding companies?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... holding company's business, a component based on its organizational form, and a component based on its...-site supervision of a noncomplex, low risk savings and loan holding company structure. OTS will... company is the registered holding company at the highest level of ownership in a holding company structure...

  19. Determination of chemical properties in 'calçot' (Allium cepa L.) by near infrared spectroscopy and multivariate calibration.

    PubMed

    Sans, Silvia; Ferré, Joan; Boqué, Ricard; Sabaté, José; Casals, Joan; Simó, Joan

    2018-10-01

    'Calçots', the immature floral stems of second-year onion resprouts, are an economically important traditional crop in Catalonia (Spain). Classical approaches to evaluating the chemical properties of 'calçots' are time consuming and expensive; near-infrared spectroscopy (NIRS) may be faster and cheaper. We used NIRS to develop partial least square (PLS) models to predict dry matter, soluble solid content, titratable acidity, and ash content in cooked 'calçots'. To guarantee the robustness of the models, calibration samples were grown and analyzed in a first season (2014-15) and validation samples in a second season (2015-16). NIRS on puree spectra estimated dry matter and soluble solid content with excellent accuracy (R 2 pred  = 0.953, 0.985 and RPD = 4.571, 8.068, respectively). However, good estimation of titratable acidity and ash content required using ground dried puree spectra (R 2 pred  = 0.852, 0.820 and RPD = 2.590, 1.987, respectively). NIRS can be a helpful tool for 'calçots' breeding and quality control. Copyright © 2018 Elsevier Ltd. All rights reserved.

  20. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... holding company? (a) If the most recent examination rating assigned to the responsible savings and loan holding company (or most recent examination rating assigned to any savings and loan holding company in the... system. OTS uses the most recent rating of which the savings and loan holding company has been notified...

  1. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... holding company? (a) If the most recent examination rating assigned to the responsible savings and loan holding company (or most recent examination rating assigned to any savings and loan holding company in the... system. OTS uses the most recent rating of which the savings and loan holding company has been notified...

  2. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... holding company? (a) If the most recent examination rating assigned to the responsible savings and loan holding company (or most recent examination rating assigned to any savings and loan holding company in the... system. OTS uses the most recent rating of which the savings and loan holding company has been notified...

  3. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... holding company? (a) If the most recent examination rating assigned to the responsible savings and loan holding company (or most recent examination rating assigned to any savings and loan holding company in the... system. OTS uses the most recent rating of which the savings and loan holding company has been notified...

  4. 12 CFR 502.29 - How does OTS determine the condition component for a savings and loan holding company?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... holding company? (a) If the most recent examination rating assigned to the responsible savings and loan holding company (or most recent examination rating assigned to any savings and loan holding company in the... system. OTS uses the most recent rating of which the savings and loan holding company has been notified...

  5. Over-Expression of the Mycobacterial Trehalose-Phosphate Phosphatase OtsB2 Results in a Defect in Macrophage Phagocytosis Associated with Increased Mycobacterial-Macrophage Adhesion

    PubMed Central

    Li, Hao; Wu, Mei; Shi, Yan; Javid, Babak

    2016-01-01

    Trehalose-6-phosphate phosphatase (OtsB2) is involved in the OtsAB trehalose synthesis pathway to produce free trehalose and is strictly essential for mycobacterial growth. We wished to determine the effects of OtsB2 expression on mycobacterial phenotypes such as growth, phagocytosis and survival in macrophages. Mycobacterium bovis-bacillus calmette-guerin (BCG) over-expressing OtsB2 were able to better survive in stationary phase. Over-expression of OtsB2 led to a decrease in phagocytosis but not survival in THP-1 macrophage-like cells, and this was not due to a decrease in general macrophage phagocytic activity. Surprisingly, when we investigated macrophage–mycobacterial interactions by flow cytometry and atomic force microscopy, we discovered that BCG over-expressing OtsB2 have stronger binding to THP-1 cells than wild-type BCG. These results suggest that altering OtsB2 expression has implications for mycobacterial host–pathogen interactions. Macrophage–mycobacteria phagocytic interactions are complex and merit further study. PMID:27867377

  6. The prognostic value of long noncoding RNA Sox2ot expression in various cancers: A systematic review and meta-analysis.

    PubMed

    Song, Xiaoyang; Yao, Hongyan; Liu, Jinlin; Wang, Qiang

    2018-05-19

    Several investigations have explored the prognostic value of long noncoding RNA Sox2 overlapping transcript (lncRNA Sox2ot) expression in human cancers, however, with inconsistent results. The aim of this study was to evaluate the prognostic role of lncRNA Sox2ot expression in various cancers. PubMed, Web of Science, Embase, and Cochrane Library were comprehensively searched to retrieve relevant studies. The relationships between lncRNA Sox2ot expression and prognostic parameters were detected, including overall survival (OS), tumor differentiation, clinical stage, distant metastasis, lymph node metastasis and so on. A total of 10 studies involving 943 cancer patients were finally included into the study. High lncRNA Sox2ot expression was significantly related to shorter OS in cancers (HR = 2.06, 95%CI = 1.67-2.55, P < 0.01). The cancer patients with high lncRNA Sox2ot expression tended to have worse tumor differentiation (P = 0.04), advanced clinical stage (P < 0.01), earlier distant metastasis (P < 0.01), and earlier lymph node metastasis (P = 0.01) compared to those with low lncRNA Sox2ot expression. However, there was no distinct correlation between lncRNA Sox2ot expression and age (P = 0.87), gender (P = 0.48), tumor size (P = 0.08), or vascular invasion (P = 0.07). High lncRNA Sox2ot expression was significantly associated with worse OS, advanced clinical stage, worse tumor differentiation, earlier distant metastasis, and earlier lymph node metastasis in various cancers. LncRNA Sox2ot expression might a promising prognostic factor in various cancers. Copyright © 2018 Elsevier B.V. All rights reserved.

  7. Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS.

    PubMed

    Salgado, Francisco; Albornoz, Laura; Cortéz, Carmen; Stashenko, Elena; Urrea-Vallejo, Kelly; Nagles, Edgar; Galicia-Virviescas, Cesar; Cornejo, Alberto; Ardiles, Alejandro; Simirgiotis, Mario; García-Beltrán, Olimpo; Areche, Carlos

    2017-12-27

    Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species ( U. barbata , U. antarctica , U. rubicunda and U. subfloridana ), a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata , 21 in U. antarctica , 38 in U. rubicunda and 37 in U. subfloridana . Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies.

  8. Rotational reorientation dynamics of Aerosol-OT reverse micelles formed in near-critical propane

    SciTech Connect

    Heitz, M.P.; Bright, F.V.

    1996-06-01

    The rotational reorientation kinetics of two fluorescent solutes (rhodamine 6G, R6G, and rhodamine 101, R101) have been determined in sodium bis(2-ethylhexyl) sulfosuccinate (Aerosol-OT, AOT) reverse micelles formed in liquid and near-critical propane. We show that the amount of water loading ([water]/[AOT], R), continuous phase density, and temperature all influence the solute rotational dynamics. In all cases, the decay of anisotropy data (i.e., frequency-dependent differential polarized phase angle and polarized modulation ratio) are well described by a bi-exponential decay law. We find that the faster rotational correlation times are similar to but slightly less than the values predicted for an individualmore » AOT reverse micelle rotating in propane. The recovered rotational correlation times range from 200 to 500 ps depending on experimental conditions. This faster rotational process is explained in terms of lateral diffusion of the fluorophore along the water/headgroup interfacial region within the reverse micelle. The recovered values for the slower rotational correlation times range from 7 to 18 ns. These larger rotational reorientation times are assigned to varying micelle-micelle (i.e., tail-tail) interactions in the low-density, highly compressible fluid region. We also quantify the contribution of the reverse micellar {open_quotes}aggregate{close_quotes} to the total decay of anisotropy. {copyright} {ital 1996} {ital Society for Applied Spectroscopy}« less

  9. OT2_jcernich_9: Time Variability of Thermal Molecular Line Emission in IRC+10216

    NASA Astrophysics Data System (ADS)

    Cernicharo, J.

    2011-09-01

    We have found during our GT line survey of IRC+10216 and the search for hydrides (OT1 proposal) that some molecular lines present a strong intensity variation with time due to the role of infrared pumping. For some lines the intensity change in six months reaches a factor 3 (CCH). We have checked that the effect is not instrumental and than it arises from physical processes ignored so far in the radiative transfer models. We propose to observe the CCH and HNC lines within bands 1a-5b of HIFI every four months (three observing slots) to allow a detailed study of the variation of thermal molecular emission, and dust emission, in this prototype of AGB C-rich object. The settings will also provide, as a bonus, many lines of SiO, SiS, CS, HCN, CO and 13CO for which intensity variations of up to 30% have been found. In addition, a few specificc settings for HCN and CO will complete the observations. SPIRE and PACS observations will complement, with lower spectral resolution, the whole spectrum of each of these molecules and will provide a global view of the total intensity change of these lines with time. A crude estimate of the distance could be also obtained from the observed time lags between the blue and red parts of the line profiles observed with HIFI.

  10. Molecular characterization of the group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3.

    PubMed

    Okochi, Mina; Matsuzaki, Hiroki; Nomura, Tomoko; Ishii, Noriyuki; Yohda, Masafumi

    2005-04-01

    The group II chaperonin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 (PhCPN) and its functional cooperation with the cognate prefoldin were investigated. PhCPN existed as a homo-oligomer in a double-ring structure, which protected the citrate synthase of a porcine heart from thermal aggregation at 45 degrees C, and did the same on the isopropylmalate dehydrogenase (IPMDH) of a thermophilic bacterium, Thermus thermophilus HB8, at 90 degrees C. PhCPN also enhanced the refolding of green fluorescent protein (GFP), which had been unfolded by low pH, in an ATP-dependent manner. Unexpectedly, functional cooperation between PhCPN and Pyrococcus prefoldin (PhPFD) in the refolding of GFP was not observed. Instead, cooperation between PhCPN and PhPFD was observed in the refolding of IPMDH unfolded with guanidine hydrochloride. Although PhCPN alone was not effective in the refolding of IPMDH, the refolding efficiency was enhanced by the cooperation of PhCPN with PhPFD.

  11. MASTER OT J132104.04+560957.8: A Polar with Absorption–Emission Line Reversals

    NASA Astrophysics Data System (ADS)

    Littlefield, Colin; Garnavich, Peter; Hoyt, Taylor J.; Kennedy, Mark

    2018-01-01

    We present time-resolved photometry and spectroscopy of the recently classified polar MASTER OT J132104.04+560957.8. The spectrum shows a smooth, nonthermal continuum at the time of maximum light, without any individually discernible cyclotron harmonics. Using homogenous cyclotron modeling, we interpret this as cyclotron radiation whose individual harmonics have blended together, and on this basis, we loosely constrain the magnetic-field strength to be less than ∼30 MG. In addition, for about one-tenth of the orbital period, the Balmer and He I emission lines transition into absorption features, with He II developing an absorption core. We use our observations of this phenomenon to test theoretical models of the accretion curtain and conclude that the H and He I lines are produced throughout the curtain, in contravention of theoretical predictions of separate H and He I line-forming regions. Moreover, a significant amount of He II emission originates within the accretion curtain, implying that the curtain is significantly hotter than expected from theory. Finally, we comment on the object’s long-term photometry, including evidence that it recently transitioned into a prolonged, exceptionally stable high state following a potentially decades-long low state.

  12. Treatment of geographic atrophy by the topical administration of OT-551: results of a phase II clinical trial.

    PubMed

    Wong, Wai T; Kam, Waynekid; Cunningham, Denise; Harrington, Molly; Hammel, Keri; Meyerle, Catherine B; Cukras, Catherine; Chew, Emily Y; Sadda, Srinivas R; Ferris, Frederick L

    2010-12-01

    To investigate the safety and preliminary efficacy of OT-551, a disubstituted hydroxylamine with antioxidant properties, for the treatment of geographic atrophy (GA), the advanced atrophic form of age-related macular degeneration (AMD). The study was a single-center, open-label phase II trial, enrolling 10 participants with bilateral GA. Topical 0.45% OT-551 was administered in one randomly assigned eye three times daily for 2 years. Safety measures were assessed by complete ophthalmic examination, fundus photography, and review of symptoms. The primary efficacy outcome measure was the change in best corrected visual acuity at 24 months. Secondary efficacy measures included changes in area of GA, contrast sensitivity, microperimetry measurements, and total drusen area from baseline. Study drug was well tolerated and was associated with few adverse events. The mean change in BCVA at 2 years was +0.2 ± 13.3 letters in the study eyes and -11.3 ± 7.6 letters in fellow eyes (P = 0.0259). However, no statistically significant differences were found between the study and fellow eyes for all other secondary outcome measures. OT-551 was well tolerated by study participants and was not associated with any serious adverse effects. Efficacy measurements in this small study indicate a possible effect in maintaining visual acuity. However, the absence of significant effects on other outcomes measures in this study suggests that OT-551, in the current concentration and mode of delivery, may have limited or no benefit as a treatment for GA (ClinicalTrials.gov number, NCT00306488).

  13. A C-Te-based binary OTS device exhibiting excellent performance and high thermal stability for selector application.

    PubMed

    Chekol, Solomon Amsalu; Yoo, Jongmyung; Park, Jaehyuk; Song, Jeonghwan; Sung, Changhyuck; Hwang, Hyunsang

    2018-08-24

    In this letter, we demonstrate a new binary ovonic threshold switching (OTS) selector device scalable down to ø30 nm based on C-Te. Our proposed selector device exhibits outstanding performance such as a high switching ratio (I on /I off  > 10 5 ), an extremely low off-current (∼1 nA), an extremely fast operating speed of <10 ns (transition time of <2 ns and delay time of <8 ns), high endurance (10 9 ), and high thermal stability (>450 °C). The observed high thermal stability is caused by the relatively small atomic size of C, compared to Te, which can effectively suppress the segregation and crystallization of Te in the OTS film. Furthermore, to confirm the functionality of the selector in a crossbar array, we evaluated a 1S-1R device by integrating our OTS device with a ReRAM (resistive random access memory) device. The 1S-1R integrated device exhibits a successful suppression of leakage current at the half-selected cell and shows an excellent read-out margin (>2 12 word lines) in a fast read operation.

  14. VizieR Online Data Catalog: Follow-up photometry of M101 OT2015-1 (Blagorodnova+, 2017)

    NASA Astrophysics Data System (ADS)

    Blagorodnova, N.; Kotak, R.; Polshaw, J.; Kasliwal, M. M.; Cao, Y.; Cody, A. M.; Doran, G. B.; Elias-Rosa, N.; Fraser, M.; Fremling, C.; Gonzalez-Fernandez, C.; Harmanen, J.; Jencson, J.; Kankare, E.; Kudritzki, R.-P.; Kulkarni, S. R.; Magnier, E.; Manulis, I.; Masci, F. J.; Mattila, S.; Nugent, P.; Ochner, P.; Pastorello, A.; Reynolds, T.; Smith, K.; Sollerman, J.; Taddia, F.; Terreran, G.; Tomasella, L.; Turatto, M.; Vreeswijk, P. M.; Wozniak, P.; Zaggia, S.

    2017-07-01

    The location of M101-OT2015-1 has been serendipitously imaged by numerous telescopes and instruments over the last 15 years (from 2000 to 2015). Our best quality pre-discovery image (seeing of 0.55") is an r-band exposure at -3625 days pre-peak from the Canada-France-Hawaii Telescope (CFHT). The historical optical data for M101-OT was retrieved from the CFHT MegaPrime and CFHT12K/Mosaic, using single and combined exposures, Pan-STARRS-1/GPC1 (PS1), Isaac Newton Telescope/Wide Field Camera (INT/WFC), and Sloan Digital Sky Survey (SDSS) DR 10 (Ahn+ 2014, see V/147). Unfortunately, there are no HST images covering the location of the source. Post-discovery optical magnitudes were obtained from the reported followup astronomer's telegrams (ATels), Liverpool Telescope (LT), the Nordic Optical Telescope (NOT), and the Palomar P48 and P60 telescopes. The infrared data were retrieved from CFHT/WIRCam, UKIRT/WFCAM, and the Spitzer Infrared Array Camera in 3.6 and 4.5um as part of the SPitzer InfraRed Intensive Transients Survey (SPIRITS) (Kasliwal+, 2017ApJ...839...88K). Details of pre-discovery photometry and post-discovery optical photometry may be found in the Appendices Tables 1 and 2, respectively. We obtained spectra of M101-OT using a range of facilities in 2015 Feb-Jul. (3 data files).

  15. Transcriptional role of androgen receptor in the expression of long non-coding RNA Sox2OT in neurogenesis

    PubMed Central

    Tosetti, Valentina; Sassone, Jenny; Ferri, Anna L. M.; Taiana, Michela; Bedini, Gloria; Nava, Sara; Brenna, Greta; Di Resta, Chiara; Pareyson, Davide; Di Giulio, Anna Maria; Carelli, Stephana

    2017-01-01

    The complex architecture of adult brain derives from tightly regulated migration and differentiation of precursor cells generated during embryonic neurogenesis. Changes at transcriptional level of genes that regulate migration and differentiation may lead to neurodevelopmental disorders. Androgen receptor (AR) is a transcription factor that is already expressed during early embryonic days. However, AR role in the regulation of gene expression at early embryonic stage is yet to be determinate. Long non-coding RNA (lncRNA) Sox2 overlapping transcript (Sox2OT) plays a crucial role in gene expression control during development but its transcriptional regulation is still to be clearly defined. Here, using Bicalutamide in order to pharmacologically inactivated AR, we investigated whether AR participates in the regulation of the transcription of the lncRNASox2OTat early embryonic stage. We identified a new DNA binding region upstream of Sox2 locus containing three androgen response elements (ARE), and found that AR binds such a sequence in embryonic neural stem cells and in mouse embryonic brain. Our data suggest that through this binding, AR can promote the RNA polymerase II dependent transcription of Sox2OT. Our findings also suggest that AR participates in embryonic neurogenesis through transcriptional control of the long non-coding RNA Sox2OT. PMID:28704421

  16. Transcriptional role of androgen receptor in the expression of long non-coding RNA Sox2OT in neurogenesis.

    PubMed

    Tosetti, Valentina; Sassone, Jenny; Ferri, Anna L M; Taiana, Michela; Bedini, Gloria; Nava, Sara; Brenna, Greta; Di Resta, Chiara; Pareyson, Davide; Di Giulio, Anna Maria; Carelli, Stephana; Parati, Eugenio A; Gorio, Alfredo

    2017-01-01

    The complex architecture of adult brain derives from tightly regulated migration and differentiation of precursor cells generated during embryonic neurogenesis. Changes at transcriptional level of genes that regulate migration and differentiation may lead to neurodevelopmental disorders. Androgen receptor (AR) is a transcription factor that is already expressed during early embryonic days. However, AR role in the regulation of gene expression at early embryonic stage is yet to be determinate. Long non-coding RNA (lncRNA) Sox2 overlapping transcript (Sox2OT) plays a crucial role in gene expression control during development but its transcriptional regulation is still to be clearly defined. Here, using Bicalutamide in order to pharmacologically inactivated AR, we investigated whether AR participates in the regulation of the transcription of the lncRNASox2OTat early embryonic stage. We identified a new DNA binding region upstream of Sox2 locus containing three androgen response elements (ARE), and found that AR binds such a sequence in embryonic neural stem cells and in mouse embryonic brain. Our data suggest that through this binding, AR can promote the RNA polymerase II dependent transcription of Sox2OT. Our findings also suggest that AR participates in embryonic neurogenesis through transcriptional control of the long non-coding RNA Sox2OT.

  17. Multiseasonal Tree Crown Structure Mapping with Point Clouds from OTS Quadrocopter Systems

    NASA Astrophysics Data System (ADS)

    Hese, S.; Behrendt, F.

    2017-08-01

    OTF (Off The Shelf) quadro copter systems provide a cost effective (below 2000 Euro), flexible and mobile platform for high resolution point cloud mapping. Various studies showed the full potential of these small and flexible platforms. Especially in very tight and complex 3D environments the automatic obstacle avoidance, low copter weight, long flight times and precise maneuvering are important advantages of these small OTS systems in comparison with larger octocopter systems. This study examines the potential of the DJI Phantom 4 pro series and the Phantom 3A series for within-stand and forest tree crown 3D point cloud mapping using both within stand oblique imaging in different altitude levels and data captured from a nadir perspective. On a test site in Brandenburg/Germany a beach crown was selected and measured with 3 different altitude levels in Point Of Interest (POI) mode with oblique data capturing and deriving one nadir mosaic created with 85/85 % overlap using Drone Deploy automatic mapping software. Three different flight campaigns were performed, one in September 2016 (leaf-on), one in March 2017 (leaf-off) and one in May 2017 (leaf-on) to derive point clouds from different crown structure and phenological situations - covering the leaf-on and leafoff status of the tree crown. After height correction, the point clouds where used with GPS geo referencing to calculate voxel based densities on 50 × 10 × 10 cm voxel definitions using a topological network of chessboard image objects in 0,5 m height steps in an object based image processing environment. Comparison between leaf-off and leaf-on status was done on volume pixel definitions comparing the attributed point densities per volume and plotting the resulting values as a function of distance to the crown center. In the leaf-off status SFM (structure from motion) algorithms clearly identified the central stem and also secondary branch systems. While the

  18. Downregulation of Long Non-Coding RNA Kcnq1ot1: An Important Mechanism of Arsenic Trioxide-Induced Long QT Syndrome.

    PubMed

    Jiang, Yanan; Du, Weijie; Chu, Qun; Qin, Ying; Tuguzbaeva, Gulnara; Wang, Hui; Li, Anqi; Li, Guiyang; Li, Yanyao; Chai, Lu; Yue, Er; Sun, Xi; Wang, Zhiguo; Pavlov, Valentin; Yang, Baofeng; Bai, Yunlong

    2018-01-01

    Arsenic trioxide (ATO) is a known anti-acute promyelocytic leukemia (APL) reagent, whose clinical applications are limited by its serious cardiac toxicity and fatal adverse effects, such as sudden cardiac death resulting from long QT syndrome (LQTS). The mechanisms of cardiac arrhythmia due to ATO exposure still need to be elucidated. Long non-coding RNAs (lncRNAs) are emerging as major regulators of various pathophysiological processes. This study aimed to explore the involvement of lncRNAs in ATO-induced LQTS in vivo and in vitro. For in vivo experiments, mice were administered ATO through the tail vein. For in vitro experiments, ATO was added to the culture medium of primary cultured neonatal mouse cardiomyocytes. To evaluate the effect of lncRNA Kcnq1ot1, siRNA and lentivirus-shRNA were synthesized to knockdown lncRNA Kcnq1ot1. After ATO treatment, the Kcnq1ot1 and Kcnq1 expression levels were down regulated. lncRNA Kcnq1ot1 knockdown prolonged the action potential duration (APD) in vitro and exerted LQTS in vivo. Correspondingly, Kcnq1 expression was decreased after silencing lncRNA Kcnq1ot1. However, the knockdown of Kcnq1 exerted no effect on lncRNA Kcnq1ot1 expression. To our knowledge, this report is the first to demonstrate that lncRNA Kcnq1ot1 downregulation is responsible for QT interval prolongation induced by ATO at least partially by repressing Kcnq1 expression. lncRNA Kcnq1ot1 has important pathophysiological functions in the heart and could become a novel antiarrhythmic target. © 2018 The Author(s). Published by S. Karger AG, Basel.

  19. A simple procedure for construction of the orthonormal basis vectors of irreducible representations of O(5) in the OT (3) ⊗ON (2) basis

    NASA Astrophysics Data System (ADS)

    Pan, Feng; Ding, Xiaoxue; Launey, Kristina D.; Draayer, J. P.

    2018-06-01

    A simple and effective algebraic isospin projection procedure for constructing orthonormal basis vectors of irreducible representations of O (5) ⊃OT (3) ⊗ON (2) from those in the canonical O (5) ⊃ SUΛ (2) ⊗ SUI (2) basis is outlined. The expansion coefficients are components of null space vectors of the projection matrix with four nonzero elements in each row in general. Explicit formulae for evaluating OT (3)-reduced matrix elements of O (5) generators are derived.

  20. Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3.

    PubMed

    Terada, Atsushi; Honda, Takashi; Fukuhara, Hideo; Hada, Kazumasa; Kimura, Makoto

    2006-08-01

    Ribonuclease P (RNase P) is a ribonucleoprotein complex involved in the processing of the 5'-leader sequence of precursor tRNA (pre-tRNA). Our earlier study revealed that RNase P RNA (pRNA) and five proteins (PhoPop5, PhoRpp38, PhoRpp21, PhoRpp29, and PhoRpp30) in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 reconstituted RNase P activity that exhibits enzymatic properties like those of the authentic enzyme. In present study, we investigated involvement of the individual proteins in RNase P activity. Two particles (R-3Ps), in which pRNA was mixed with three proteins, PhoPop5, PhoRpp30, and PhoRpp38 or PhoPop5, PhoRpp30, and PhoRpp21 showed a detectable RNase P activity, and five reconstituted particles (R-4Ps) composed of pRNA and four proteins exhibited RNase P activity, albeit at reduced level compared to that of the reconstituted particle (R-5P) composed of pRNA and five proteins. Time-course analysis of the RNase P activities of R-4Ps indicated that the R-4Ps lacking PhoPop5, PhoRpp21, or PhoRpp30 had virtually reduced activity, while omission of PhoRpp29 or PhoRpp38 had a slight effect on the activity. The results indicate that the proteins contribute to RNase P activity in order of PhoPop5 > PhoRpp30 > PhoRpp21 > PhoRpp29 > PhoRpp38. It was further found that R-4Ps showed a characteristic Mg2+ ion dependency approximately identical to that of R-5P. However, R-4Ps had optimum temperature of around at 55 degrees C which is lower than 70 degrees C for R-5P. Together, it is suggested that the P. horikoshii RNase P proteins are predominantly involved in optimization of the pRNA conformation, though they are individually dispensable for RNase P activity in vitro.

  1. OT2_eegami_6: SPIRE Snapshot Survey II: Using SPT/CODEX Massive Clusters as Powerful Gravitational Lenses

    NASA Astrophysics Data System (ADS)

    Egami, E.

    2011-09-01

    On the extragalactic side, one of the most remarkable results coming out of Herschel is the discovery of extremely bright (>100 mJy in the SPIRE bands) gravitationally lensed galaxies. The great sensitivity and mapping speed of SPIRE have enabled us to find these rare extraordinary objects. What is truly exciting about these bright lensed galaxies is that they enable a variety of detailed multi-wavelength follow-up observations, shedding new light on the physical properties of these high-redshift sources. In this regard, our OT1 program, "SPIRE Snapshot Survey of Massive Galaxy Clusters" turned out to be a great success. After imaging ~50 galaxies out of 279 in the program, we have already found two spectacularly bright lensed galaxies, one of which is at a redshift of 4.69. This type of cluster-lensed sources are not only bright but also spatially stretched over a large scale, so ALMA (or NOEMA in the north) is likely to be able to study them at the level of individual GMCs. Such studies will open up a new frontier in the study of high-redshift galaxies. Here, we propose to extend this highly efficient and effective survey of gravitationally lensed galaxies to another 353 clusters carefully chosen from the SPT and CODEX cluster samples. These samples contain newly discovered high-redshift (z>0.3) massive (>3-4e14 Msun) clusters, which can be used as powerful gravitational lenses to magnify sources at high redshift. With the OT1 and OT2 surveys together, we expect to find ~20 highly magnified SPIRE sources with exceptional brightnesses (assuming a discovery rate of ~1/30). Such a unique sample of extraordinary objects will enable a variety of follow-up sciences, and will therefore remain as a great legacy of the Herschel mission for years to come.

  2. DISCOVERY OF PRECURSOR LUMINOUS BLUE VARIABLE OUTBURSTS IN TWO RECENT OPTICAL TRANSIENTS: THE FITFULLY VARIABLE MISSING LINKS UGC 2773-OT AND SN 2009ip

    SciTech Connect

    Smith, Nathan; Miller, Adam; Li Weidong

    2010-04-15

    We present progenitor-star detections, light curves, and optical spectra of supernova (SN) 2009ip and the 2009 optical transient in UGC 2773 (U2773-OT), which were not genuine SNe. Precursor variability in the decade before outburst indicates that both of the progenitor stars were luminous blue variables (LBVs). Their pre-outburst light curves resemble the S Doradus phases that preceded giant eruptions of the prototypical LBVs {eta} Carinae and SN 1954J (V12 in NGC 2403), with intermediate progenitor luminosities. Hubble Space Telescope detections a decade before discovery indicate that the SN 2009ip and U2773-OT progenitors were supergiants with likely initial masses of 50-80more » M {sub sun} and {approx}>20 M {sub sun}, respectively. Both outbursts had spectra befitting known LBVs, although in different physical states. SN 2009ip exhibited a hot LBV spectrum with characteristic speeds of 550 km s{sup -1}, plus evidence for faster material up to 5000 km s{sup -1}, resembling the slow Homunculus and fast blast wave of {eta} Carinae. In contrast, U2773-OT shows a forest of narrow absorption and emission lines comparable to that of S Dor in its cool state, plus [Ca II] emission and an infrared excess indicative of dust, similar to SN 2008S and the 2008 optical transient in NGC 300 (N300-OT). The [Ca II] emission is probably tied to a dusty pre-outburst environment, and is not a distinguishing property of the outburst mechanism. The LBV nature of SN 2009ip and U2773-OT may provide a critical link between historical LBV eruptions, while U2773-OT may provide a link between LBVs and the unusual dust-obscured transients SN 2008S and N300-OT. Future searches will uncover more examples of precursor LBV variability of this kind, providing key clues that may help unravel the instability driving LBV eruptions in massive stars.« less

  3. Targeting the T-Lak cell originated protein kinase by OTS964 shrinks the size of power-law coded heterogeneous glioma stem cell populations

    PubMed Central

    Sugimori, Michiya; Hayakawa, Yumiko; Koh, Masaki; Hayashi, Tomohide; Tamura, Ryoi; Kuroda, Satoshi

    2018-01-01

    Glioblastoma resists chemoradiotherapy, then, recurs to be a fatal space-occupying lesion. The recurrence is caused by re-growing cell populations such as glioma stem cells (GSCs), suggesting that GSC populations should be targeted. This study addressed whether a novel anti-cancer drug, OTS964, an inhibitor for T-LAK cell originated protein kinase (TOPK), is effective in reducing the size of the heterogeneous GSC populations, a power-law coded heterogeneous GSC populations consisting of glioma sphere (GS) clones, by detailing quantitative growth properties. We found that OTS964 killed GS clones while suppressing the growth of surviving GS clones, thus identifying clone-eliminating and growth-disturbing efficacies of OTS964. The efficacies led to a significant size reduction in GS populations in a dose-dependent manner. The surviving GS clones reconstructed GS populations in the following generations; the recovery of GS populations fits a recurrence after the chemotherapy. The recovering GS clones resisted the clone-eliminating effect of OTS964 in sequential exposure during the growth recovery. However, surprisingly, the resistant properties of the recovered-GS clones had been plastically canceled during self-renewal, and then the GS clones had become re-sensitive to OTS964. Thus, OTS964 targets GSCs to eliminate them or suppress their growth, resulting in shrinkage of the power-law coded GSC populations. We propose a therapy focusing on long-term control in recurrence of glioblastoma via reducing the size of the GSC populations by OTS964. PMID:29423027

  4. Targeting the T-Lak cell originated protein kinase by OTS964 shrinks the size of power-law coded heterogeneous glioma stem cell populations.

    PubMed

    Sugimori, Michiya; Hayakawa, Yumiko; Koh, Masaki; Hayashi, Tomohide; Tamura, Ryoi; Kuroda, Satoshi

    2018-01-09

    Glioblastoma resists chemoradiotherapy, then, recurs to be a fatal space-occupying lesion. The recurrence is caused by re-growing cell populations such as glioma stem cells (GSCs), suggesting that GSC populations should be targeted. This study addressed whether a novel anti-cancer drug, OTS964, an inhibitor for T-LAK cell originated protein kinase (TOPK), is effective in reducing the size of the heterogeneous GSC populations, a power-law coded heterogeneous GSC populations consisting of glioma sphere (GS) clones, by detailing quantitative growth properties. We found that OTS964 killed GS clones while suppressing the growth of surviving GS clones, thus identifying clone-eliminating and growth-disturbing efficacies of OTS964. The efficacies led to a significant size reduction in GS populations in a dose-dependent manner. The surviving GS clones reconstructed GS populations in the following generations; the recovery of GS populations fits a recurrence after the chemotherapy. The recovering GS clones resisted the clone-eliminating effect of OTS964 in sequential exposure during the growth recovery. However, surprisingly, the resistant properties of the recovered-GS clones had been plastically canceled during self-renewal, and then the GS clones had become re-sensitive to OTS964. Thus, OTS964 targets GSCs to eliminate them or suppress their growth, resulting in shrinkage of the power-law coded GSC populations. We propose a therapy focusing on long-term control in recurrence of glioblastoma via reducing the size of the GSC populations by OTS964.

  5. Mechanistic Basis for High Reactivity of (salen)Co–OTs in the Hydrolytic Kinetic Resolution of Terminal Epoxides

    PubMed Central

    Nielsen, Lars P. C.; Zuend, Stephan J.; Ford, David D.; Jacobsen, Eric N.

    2012-01-01

    The (salen)Co(III)-catalyzed hydrolytic kinetic resolution (HKR) of terminal epoxides is a bimetallic process with a rate controlled by partitioning between a nucleophilic (salen)Co–OH catalyst and a Lewis acidic (salen)Co–X catalyst. The commonly used (salen)Co–OAc and (salen)Co–Cl precatalysts undergo complete and irreversible counterion addition to epoxide during the course of the epoxide hydrolysis reaction, resulting in quantitative formation of weakly Lewis acidic (salen)Co–OH, and severely diminished reaction rates in the late stages of HKR reactions. In contrast, (salen)Co–OTs maintains high reactivity over the entire course of HKR reactions. We describe here an investigation of catalyst partitioning with different (salen)Co–X precatalysts, and demonstrate that counterion addition to epoxide is reversible in the case of the (salen)Co–OTs. This reversible counterion addition results in stable partitioning between nucleophilic and Lewis acidic catalyst species, allowing highly efficient catalysis throughout the course of the HKR reaction. PMID:22292515

  6. Mechanistic basis for high reactivity of (salen)Co-OTs in the hydrolytic kinetic resolution of terminal epoxides.

    PubMed

    Nielsen, Lars P C; Zuend, Stephan J; Ford, David D; Jacobsen, Eric N

    2012-03-02

    The (salen)Co(III)-catalyzed hydrolytic kinetic resolution (HKR) of terminal epoxides is a bimetallic process with a rate controlled by partitioning between a nucleophilic (salen)Co-OH catalyst and a Lewis acidic (salen)Co-X catalyst. The commonly used (salen)Co-OAc and (salen)Co-Cl precatalysts undergo complete and irreversible counterion addition to epoxide during the course of the epoxide hydrolysis reaction, resulting in quantitative formation of weakly Lewis acidic (salen)Co-OH and severely diminished reaction rates in the late stages of HKR reactions. In contrast, (salen)Co-OTs maintains high reactivity over the entire course of HKR reactions. We describe here an investigation of catalyst partitioning with different (salen)Co-X precatalysts and demonstrate that counterion addition to epoxide is reversible in the case of the (salen)Co-OTs. This reversible counterion addition results in stable partitioning between nucleophilic and Lewis acidic catalyst species, allowing highly efficient catalysis throughout the course of the HKR reaction.

  7. OAO/MITSuME photometry of dwarf novae. II. HV Virginis and OT J012059.6+325545

    NASA Astrophysics Data System (ADS)

    Imada, Akira; Isogai, Keisuke; raki, Takahiro; Tanada, Shunsuke; Yanagisawa, Kenshi; Kawai, Nobuyuki

    2018-01-01

    We report on multicolor photometry of WZ Sge-type dwarf novae HV Vir and OT J012059.6+325545 during superoutbursts. These systems show early superhumps with mean periods of 0.057093(45) d for HV Vir and 0.057147(15) d for OT J012059.6+325545. The observed early superhumps showed a common feature that the brightness minima corresponded to the bluest peaks in color variations, which may be a ubiquitous phenomenon among early superhumps of WZ Sge-type dwarf novae. We confirmed that the amplitudes of early superhumps depend on wavelength: amplitudes with longer bandpass filters show larger values. This indicates that the light source of early superhumps is generated at the outer region of the vertically extended accretion disk. On the other hand, amplitudes of ordinary superhumps are likely to be independent of wavelength. This implies that the superhump light source is geometrically thin. We also examined color variations of ordinary superhumps and found that the bluest peaks in g΄ - Ic tend to coincide with the brightness minima, particularily in stage B superhumps. This may reflect that the pressure effect plays a dominant role during stage B superhumps.

  8. Overexpression of prefoldin from the hyperthermophilic archaeum Pyrococcus horikoshii OT3 endowed Escherichia coli with organic solvent tolerance.

    PubMed

    Okochi, Mina; Kanie, Kei; Kurimoto, Masaki; Yohda, Masafumi; Honda, Hiroyuki

    2008-06-01

    Prefoldin is a jellyfish-shaped hexameric chaperone that captures a protein-folding intermediate and transfers it to the group II chaperonin for correct folding. In this work, we characterized the organic solvent tolerance of Escherichia coli cells that overexpress prefoldin and group II chaperonin from a hyperthermophilic archeaum, Pyrococcus horikoshii OT3. The colony-forming efficiency of E. coli cells overexpressing prefoldin increased by 1,000-fold and decreased the accumulation of intracellular organic solvent. The effect was impaired by deletions of the region responsible for the chaperone function of prefoldin. Therefore, we concluded that prefoldin endows E. coli cells by preventing accumulation of intracellular organic solvent through its molecular chaperone activity.

  9. Pressure effects on enzyme reactions in mainly organic media: alpha-chymotrypsin in reversed micelles of Aerosol OT in octane.

    PubMed

    Mozhaev, V V; Bec, N; Balny, C

    1994-08-01

    Biocatalytic transformations in reversed micelles formed by anionic surfactant Aerosol OT in octane have been studied at high pressures by an example of alpha-chymotrypsin-catalyzed hydrolysis of N-carbobenzoxy-L-tyrosine p-nitrophenyl ester and N-succinyl-L-phenylalanine p-nitroanilide. For the first time it has been found that the enzyme retains high activity in these water-in-oil microemulsions up to a pressure of 2 kbar. The value of the activation volume (delta V*) for the enzyme reactions shows a dependence on the water content in the system. When the size of the micellar aqueous inner cavity (as evaluated at 1 atm) approaches the molecular size of alpha-chymotrypsin, delta V* becomes significantly different from the value in aqueous solution and in the micelles with a larger size. Possibilities of regulating the enzyme activity by pressure in systems with a low content of water are discussed.

  10. DUSTY EXPLOSIONS FROM DUSTY PROGENITORS: THE PHYSICS OF SN 2008S AND THE 2008 NGC 300-OT

    SciTech Connect

    Kochanek, C. S.

    2011-11-01

    SN 2008S and the 2008 NGC 300-OT were explosive transients of stars self-obscured by very dense, dusty stellar winds. An explosive transient with an unobserved shock breakout luminosity of order 10{sup 10} L{sub sun} is required to render the transients little obscured and visible in the optical at their peaks. Such a large breakout luminosity then implies that the progenitor stars were cool, red supergiants, most probably {approx}9 M{sub sun} extreme asymptotic giant branch stars. As the shocks generated by the explosions propagate outward through the dense wind, they produce a shock luminosity in soft X-rays that powers the long-livedmore » luminosity of the transients. Unlike typical cases of transients exploding into a surrounding circumstellar medium, the progenitor winds in these systems are optically thick to soft X-rays, easily absorb radio emission, and rapidly reform dust destroyed by the peak luminosity of the transients. As a result, X-rays are absorbed by the gas and the energy is ultimately radiated by the reformed dust. Three years post-peak, both systems are still significantly more luminous than their progenitor stars, but they are again fully shrouded by the reformed dust and only visible in the mid-IR. The high luminosity and heavy obscuration may make it difficult to determine the survival of the progenitor stars for {approx}10 years. However, our model indicates that SN 2008S, but not the NGC 300-OT, should now be a detectable X-ray source. SN 2008S has a higher estimated shock velocity and a lower density wind, so the X-rays begin to escape at a much earlier phase.« less

  11. MORPHOLOGICAL TRANSFORMATION AND DNA ADDUCT FORMATION BY BENZ[J]ACEANTHRYLENE AND ITS METABOLITES IN C3H1OT1/2CL8 CELLS: EVIDENCE FOR BOTH CYCLOPENTA-RING AND BAY REGION METABOLIC ACTIVATION PATHWAYS

    EPA Science Inventory

    Benz[j]aceanthrylene (B[j]A), a cyclopenta-fused polycyclic aromatic hydrocarbon (CP-PAH) related to 3-methylcholanthrene, has been studied to identify the major routes of metabolic activation in transformable C3H1OT1/2CLB (C3H1OT1/2) mouse embryo fibroblasts in culture. he morph...

  12. /sup 45/Ca efflux for myometrial cells: comparison of the effects of prostaglandin F/sub 2/. cap alpha. (PGF/sub 2/), oxytocin (OT) and arachidonate (A)

    SciTech Connect

    Katona, G.; Molnar, M.; Toth, M.

    1986-03-01

    The aim of this study was to measure PGF/sub 2..cap alpha../-induced Ca/sup 2 +/ release from uterine cells and to compare this to the actions of OT and A. Smooth muscle cells isolated from the uterus (shell gland) of laying hens were cultured for 7 days in M199 plus 10% fetal calf serum. The cells were treated with digitonin (20..mu..M) and preloaded with /sup 45/Ca for 40 min. Addition of PGF/sub 2..cap alpha../ caused a biphasic /sup 45/Ca-efflux. There was a small but significant /sup 45/Ca-release within 30 sec (rapid phase) followed by a larger one within 7 min (slowmore » phase). In comparison, both OT and A stimulated /sup 45/Ca efflux during a single, slow phase. The maximal effect of A was observed at < 7 min, whereas that of OT was slower, peaking after 7 min. Mepacrin, an inhibitor of A release, attenuated the action of OT without having any effect on A promoted /sup 45/Ca-efflux. Indomethacin, an inhibitor of PG synthase, failed to suppress the Ca-releasing effect of A suggesting the A itself or a lipoxygenase product may have been responsible for the observed effects. Moreover, these results provide suggestive evidence that A release is an important step in the action of various uterotonic agents converging on the mobilization of intracellular Ca.« less

  13. Glucuronidation of OTS167 in Humans Is Catalyzed by UDP-Glucuronosyltransferases UGT1A1, UGT1A3, UGT1A8, and UGT1A10

    PubMed Central

    Ramírez, Jacqueline; Mirkov, Snezana; House, Larry K.

    2015-01-01

    OTS167 is a potent maternal embryonic leucine zipper kinase inhibitor undergoing clinical testing as antineoplastic agent. We aimed to identify the UDP-glucuronosyltransferases (UGTs) involved in OTS167 metabolism, study the relationship between UGT genetic polymorphisms and hepatic OTS167 glucuronidation, and investigate the inhibitory potential of OTS167 on UGTs. Formation of a single OTS167-glucuronide (OTS167-G) was observed in pooled human liver (HLM) (Km = 3.4 ± 0.2 µM), intestinal microsomes (HIM) (Km = 1.7 ± 0.1 µM), and UGTs. UGT1A1 (64 µl/min/mg) and UGT1A8 (72 µl/min/mg) exhibited the highest intrinsic clearances (CLint) for OTS167, followed by UGT1A3 (51 µl/min/mg) and UGT1A10 (47 µl/min/mg); UGT1A9 was a minor contributor. OTS167 glucuronidation in HLM was highly correlated with thyroxine glucuronidation (r = 0.91, P < 0.0001), SN-38 glucuronidation (r = 0.79, P < 0.0001), and UGT1A1 mRNA (r = 0.72, P < 0.0001). Nilotinib (UGT1A1 inhibitor) and emodin (UGT1A8 and UGT1A10 inhibitor) exhibited the highest inhibitory effects on OTS167-G formation in HLM (68%) and HIM (47%). We hypothesize that OTS167-G is an N-glucuronide according to mass spectrometry. A significant association was found between rs6706232 and reduced OTS167-G formation (P = 0.03). No or weak UGT inhibition (range: 0–21%) was observed using clinically relevant OTS167 concentrations (0.4–2 µM). We conclude that UGT1A1 and UGT1A3 are the main UGTs responsible for hepatic formation of OTS167-G. Intestinal UGT1A1, UGT1A8, and UGT1A10 may contribute to first-pass OTS167 metabolism after oral administration. PMID:25870101

  14. Glucuronidation of OTS167 in Humans Is Catalyzed by UDP-Glucuronosyltransferases UGT1A1, UGT1A3, UGT1A8, and UGT1A10.

    PubMed

    Ramírez, Jacqueline; Mirkov, Snezana; House, Larry K; Ratain, Mark J

    2015-07-01

    OTS167 is a potent maternal embryonic leucine zipper kinase inhibitor undergoing clinical testing as antineoplastic agent. We aimed to identify the UDP-glucuronosyltransferases (UGTs) involved in OTS167 metabolism, study the relationship between UGT genetic polymorphisms and hepatic OTS167 glucuronidation, and investigate the inhibitory potential of OTS167 on UGTs. Formation of a single OTS167-glucuronide (OTS167-G) was observed in pooled human liver (HLM) (Km = 3.4 ± 0.2 µM), intestinal microsomes (HIM) (Km = 1.7 ± 0.1 µM), and UGTs. UGT1A1 (64 µl/min/mg) and UGT1A8 (72 µl/min/mg) exhibited the highest intrinsic clearances (CLint) for OTS167, followed by UGT1A3 (51 µl/min/mg) and UGT1A10 (47 µl/min/mg); UGT1A9 was a minor contributor. OTS167 glucuronidation in HLM was highly correlated with thyroxine glucuronidation (r = 0.91, P < 0.0001), SN-38 glucuronidation (r = 0.79, P < 0.0001), and UGT1A1 mRNA (r = 0.72, P < 0.0001). Nilotinib (UGT1A1 inhibitor) and emodin (UGT1A8 and UGT1A10 inhibitor) exhibited the highest inhibitory effects on OTS167-G formation in HLM (68%) and HIM (47%). We hypothesize that OTS167-G is an N-glucuronide according to mass spectrometry. A significant association was found between rs6706232 and reduced OTS167-G formation (P = 0.03). No or weak UGT inhibition (range: 0-21%) was observed using clinically relevant OTS167 concentrations (0.4-2 µM). We conclude that UGT1A1 and UGT1A3 are the main UGTs responsible for hepatic formation of OTS167-G. Intestinal UGT1A1, UGT1A8, and UGT1A10 may contribute to first-pass OTS167 metabolism after oral administration. Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics.

  15. SOX2OT variant 7 contributes to the synergistic interaction between EGCG and Doxorubicin to kill osteosarcoma via autophagy and stemness inhibition.

    PubMed

    Wang, Wanchun; Chen, Ding; Zhu, Kewei

    2018-02-23

    Doxorubicin is the preferred chemotherapeuticdrug for osteosarcoma treatment of which clinical efficacy is limited because of its chemo-resistance and cardiac toxicity. It is necessary to develop the combination regimen with complementary molecular mechanisms to reduce the side effects and enhance sensitivity of Doxorubicin. EGCG is a polyphenol in green tea with antitumor bioactivity,which has been found that its combination with certain chemotherapeutic drugs could improve the antitumor efficiency. In this study, MTT assay was used to detect the cell growth inhibition The CD133+/CD44+ cells were isolated from U2OS and SaoS2 cell lines using magnetic-activated cell sorting and identified by flow cytometry analysis. qRT-PCR was used for determining the relative mRNA levels of key genes. Immunofluorescence was performed to evaluate the autophagy flux alterations. Self-renewal ability was accessed by sphere-forming assay. Tumorigenicity in nude mice was preformed to evaluate tumorigenicity in vivo. We found that EGCG targeting LncRNA SOX2OT variant 7 produced synergistic effects with Doxorubicin on osteosarcoma cell growth inhibition. On the one hand, EGCG could reduce the Doxorubicin-induced pro-survival autophagy through decreasing SOX2OT variant 7 to improve the growth inhibition of Doxorubicin. On the other hand, EGCG could partially inactivate Notch3/DLL3 signaling cascade targeting SOX2OT variant 7 to reduce the stemness then abated drug-resistance of osteosarcoma cells. This study will help to reveal the molecular mechanisms of synergistic effects of EGCG and Doxorubicin on OS chemotherapy and improve the clinical efficacy of chemotherapy as well as provide a basis for developing antitumor drugs targeting osteosarcoma stem cells.

  16. High-cadence Multi-color Observations of the Dwarf Nova KSP-OT-201503a by the KMTNet Supernova Program

    NASA Astrophysics Data System (ADS)

    Brown, Shannon; Moon, Dae-Sik; Ni, Yuan Qi; Drout, Maria; Antoniadis, John; Afsariardchi, Niloufar; Cha, Sang-Mok; Lee, Yongseok

    2018-06-01

    We report multicolor BVI monitoring and spectroscopic classification of the dwarf nova KSP-OT-201503a. The transient was detected by the Korean Microlensing Telescope Network (KMTNet) Supernova Program (KSP) in 2015 March, reached a peak apparent magnitude V ≃ 17.3 mag from a quiescent magnitude V ≃ 22.6 mag, and lasted for approximately 17 days. Our high-cadence sampling allows us to identify distinctive phases consisting of a rapid ascent, a main outburst composed of a flat plateau followed by a gradual dimming, and a quick decline. We observe the sharp transition between the ascent phase and main outburst phase, likely related to the deceleration of the heating front as it passes through the accretion disk. These features in the light curves indicate that the outburst is outside-in. Archival data reveal the outburst history of the source, showing at least three outbursts between 2011 and 2015. These are equally separated by approximately 25 months, though we find a recurrence time as short as 189 days is compatible with the archival data. An optical spectrum obtained 701 days from outburst peak shows prominent Balmer emission lines superimposed on a blue continuum, consistent with a cataclysmic variable in quiescence. The outburst properties of KSP-OT-201503a closely resemble those of U Gem-type dwarf novae usually associated with younger, longer-period systems above the period gap of 2–3 hr observed in cataclysmic variables. This suggests that the source may be a rare U Gem-type dwarf nova with a long recurrence time, though we are unable to rule out the possibility that KSP-OT-201503a lies below the period gap.

  17. Treatability Study in Support of Intrinsic Remediation for Site OT 24 at MacDill Air Force Base, Florida. Volume 2

    DTIC Science & Technology

    1997-01-01

    Not Sampled f PJ)ll PARSONS SAA - Some As Above Y Water level drilled LF-JENGINEERING SCIENCE.INC. Denver, Colorado L:\\45021\\,DRAWINGS\\BORELOGS\\OT-24...Remnediotion TS TOC - Top of Cosing G - GRAB MacDill Air Force Base, Florida NS- Not Sampled .PARUONU SAA - Same As Above VWater level drilled L!LJ...GRAB MacDill Air Force Base. Florida NS - Not Sampled fj•--PAMMMNuI SAA - Some As Above Y Water level drilled *NIIN I N Denver. Colorado L:\\45021

  18. Purification, crystallization and preliminary X-ray crystallographic analysis of the archaeal phosphoglycerate mutase PH0037 from Pyrococcus horikoshii OT3

    SciTech Connect

    Lokanath, Neratur K.; Kunishima, Naoki, E-mail: kunisima@spring8.or.jp

    2006-08-01

    The archaeal phosphoglycerate mutase PH0037 from P. horikoshii OT3 has been crystallized in space group R32, with unit-cell parameters a = 155.62, c = 230.35 Å. A 2.2 Å resolution data was collected at SPring-8 beamline BL26B1. Phosphoglycerate mutases catalyze the interconversion of 2-phosphoglycerate and 3-phosphoglycerate in glycolysis and gluconeogenesis pathways. The archaeal phosphoglycerate mutase PH0037 from Pyrococcus horikoshii OT3 has been overexpressed in Escherichia coli and purified. Crystals were obtained using the oil-microbatch method at 291 K. A native data set extending to a resolution of 2.2 Å has been collected and processed in space group R32. Assuming themore » presence of a dimer in the asymmetric unit, the V{sub M} value is calculated to be 3.0 Å{sup 3} Da{sup −1}, consistent with the dynamic light-scattering experiment result, which shows a dimeric state of the protein in solution. Molecular-replacement trials using the crystal structure of Bacilllus stearothermophilus phosphoglycerate mutase as a search model did not provide a satisfactory solution, indicating substantially different structures of these two phophoglycerate mutases.« less

  19. Analysis of the methylation status of the KCNQ1OT and H19 genes in leukocyte DNA for the diagnosis and prognosis of Beckwith-Wiedemann syndrome.

    PubMed

    Gaston, V; Le Bouc, Y; Soupre, V; Burglen, L; Donadieu, J; Oro, H; Audry, G; Vazquez, M P; Gicquel, C

    2001-06-01

    Beckwith-Wiedemann syndrome (BWS) is an overgrowth disorder involving developmental abnormalities, tissue and organ hyperplasia and an increased risk of embryonal tumours (most commonly Wilms tumour). This multigenic disorder is caused by dysregulation of the expression of imprinted genes in the 11p15 chromosomal region. Molecular diagnosis of BWS is currently difficult, mostly due to the large spectrum of genetic and epigenetic abnormalities. The other difficulty in managing BWS is the identification of patients at risk of tumour. An imprinted antisense transcript within KCNQ1, called KCNQ1OT (also known as LIT1), was recently shown to be normally expressed from the paternal allele. A loss of imprinting of the KCNQ1OT gene, associated with the loss of maternal allele-specific methylation of the differentially methylated region KvDMR1 has been described in BWS patients. The principal aim of this study was to evaluate the usefulness of KvDMR1 methylation analysis of leukocyte DNA for the diagnosis of BWS. The allelic status of the 11p15 region and the methylation status of the KCNQ1OT and H19 genes were investigated in leukocyte DNA from 97 patients referred for BWS and classified into two groups according to clinical data: complete BWS (CBWS) (n=61) and incomplete BWS (IBWS) (n=36). Fifty-eight (60%) patients (39/61 CBWS and 19/36 IBWS) displayed abnormal demethylation of KvDMR1. In 11 of the 56 informative cases, demethylation of KvDMR1 was related to 11p15 uniparental disomy (UPD) (nine CBWS and two IBWS). Thirteen of the 39 patients with normal methylation of KvDMR1 displayed hypermethylation of the H19 gene. These 13 patients included two siblings with 11p15 trisomy. These results show that analysis of the methylation status of KvDMR1 and the H19 gene in leukocyte DNA is useful in the diagnosis of 11p15-related overgrowth syndromes, resulting in the diagnosis of BWS in more than 70% of investigated patients. We also evaluated clinical and molecular features as

  20. Superfund record of decision (EPA Region 3): Dover Air Force Base, Oil/Water Separator at Building 918 (Site ot 40), Kent County, DE, March 28, 1995

    SciTech Connect

    NONE

    1996-02-01

    The Record of Decision presents the selected remedial action for the Oil/Water separator at Building 918 Operable Unit (Site OT 40). There are hazardous substances present in the shallow groundwater at levels below those that would cause risk. Additionally, arsenic and lead exist in soils at levels within regional background levels. The site poses a minimal risk to public health, welfare, and the environment. The No Action alternative requires no actions be taken at the site. This alternative serves as a baseline for comparison and CERCLA requires it be evaluated. It is the preferred alternative at this site because themore » other alternatives offer no substantial advantages.« less

  1. Crystallization and preliminary X-ray analysis of PH1566, a putative ribosomal RNA-processing factor from the hyperthermophilic archaeon Pyrococcus horikoshii OT3

    PubMed Central

    Jia, Min Ze; Ohtsuka, Jun; Lee, Woo Cheol; Nagata, Koji; Tanokura, Masaru

    2006-01-01

    A putative ribosomal RNA-processing factor consisting of two KH domains from Pyrococcus horikoshii OT3 (PH1566; 25 kDa) was crystallized by the sitting-drop vapour-diffusion method using PEG 3000 as the precipitant. The crystals diffracted X-rays to beyond 2.0 Å resolution using a synchrotron-radiation source. The space group of the crystals was determined as primitive orthorhombic P212121, with unit-cell parameters a = 45.9, b = 47.4, c = 95.7 Å. The crystals contain one molecule in the asymmetric unit (V M = 2.5 Å3 Da−1) and have a solvent content of 50%. PMID:16511260

  2. Multilayering of Calcium Aerosol-OT at the Mica/Water Interface Studied with Neutron Reflection: Formation of a Condensed Lamellar Phase at the CMC.

    PubMed

    Griffin, L R; Browning, K L; Lee, S Y; Skoda, M W A; Rogers, S; Clarke, S M

    2016-12-13

    Using specular neutron reflection, the adsorption of sodium and calcium salts of the surfactant bis(2-ethylhexyl) sulfosuccinate (Aerosol-OT or AOT) has been studied at the mica/water interface at concentrations between 0.1 and 2 CMC. The pH dependence of the adsorption was also probed. No evidence of the adsorption of Na(AOT) was found even at the critical micelle concentration (CMC) while the calcium salt was found to adsorb significantly at concentrations of 0.5 CMC and above. This interesting and somewhat unexpected finding demonstrates that counterion identity may be used to tune the adsorption of anionic surfactants on anionic surfaces. At the CMC, three condensed bilayers of Ca(AOT) 2 were adsorbed at pH 7 and 9 and four bilayers adsorbed at pH 4. Multilayering at the CMC of Ca(AOT) 2 on the mica surface is an unusual feature of this surfactant/surface combination. Only single bilayer adsorption has been observed at other surfaces at the CMC. We suggest this arises from the high charge density of mica which must provide an excellent template for the surfactant.

  3. MASTER OT J004207.99+405501.1/M31LRN 2015 luminous red nova in M31: discovery, light curve, hydrodynamics and evolution

    NASA Astrophysics Data System (ADS)

    Lipunov, V. M.; Blinnikov, S.; Gorbovskoy, E.; Tutukov, A.; Baklanov, P.; Krushinski, V.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Kornilov, V.; Gorbunov, I.; Shumkov, V.; Vladimirov, V.; Gress, O.; Budnev, N. M.; Ivanov, K.; Tlatov, A.; Gabovich, A.; Yurkov, V.; Sergienko, Yu.; Zalozhnykh, I.

    2017-09-01

    We report the discovery and multicolour (VRIW) photometry of the rare explosive star MASTER OT J004207.99+405501.1 - a luminous red nova - in the Andromeda galaxy M31N2015-01a. We use our original light curve acquired with identical MASTER Global Robotic Net telescopes in one photometric system: VRI during the first 30 d and W (unfiltered) during 70 d. Also, we added published multicolour photometry data to estimate the mass and energy of the ejected shell and we discuss the likely formation scenarios of outbursts of this type. We propose an interpretation of the explosion that is consistent with an evolutionary scenario where the merging of stellar components or the disruption of the common envelope of a close binary can explain some luminous red novae. Radiative hydrodynamic simulations of a luminous red nova were carried out in extended parameter space to fit its light curves. We find that the multicolour passband light curves of the luminous red nova are consistent with an initial common envelope radius of 10 R⊙, a merger mass of 3 M⊙ and an explosion energy of 3 × 1048 erg. As a result, the phenomenon of novae consists of two classes: classical nuclear novae and more rare events (red novae) connected with the loss of compact common envelopes.

  4. Can H-aggregates serve as light-harvesting antennae? Triplet-triplet energy transfer between excited aggregates and monomer thionine in aerosol-OT solutions

    SciTech Connect

    Das, S.; Kamat, P.V.

    1999-01-07

    The cationic dye thionine undergoes slow dissolution in aerosol-OT (AOT) containing solutions of heptane and toluene. By controlling the ratio of [dye]/[AOT], it is possible to obtain varying amounts of monomer, dimer, and higher order aggregates (trimer) in dilute dye solutions. The thionine aggregates exhibit characteristic absorption maxima at 565 and 530 nm for the dimer and trimer forms, respectively. The singlet excited states of these dye aggregates are short-lived ({tau} = 40--63 ps) as they undergo efficient intersystem crossing to generate the triplet excited states. Triplet energy transfer from the excited dye aggregates to monomeric thionine molecules was observedmore » upon excitation with a 532 nm laser pulse. Pulse radiolysis experiments, in which the excited triplet states were generated indirectly, also confirm the finding that the triplet energy cascades down from excited trimer to dimer to monomeric dye. These studies demonstrate the possibility of using H-type dye aggregates as antenna molecules to harvest light energy whereby the aggregate molecules absorb light in different spectral regions and subsequently transfer energy to the monomeric dye.« less

  5. The structure of subunit E of the Pyrococcus horikoshii OT3 A-ATP synthase gives insight into the elasticity of the peripheral stalk.

    PubMed

    Balakrishna, Asha Manikkoth; Hunke, Cornelia; Grüber, Gerhard

    2012-07-13

    A(1)A(O) ATP synthases are the major energy converters of archaea. They are composed of an A(1) region that synthesizes ATP and an integral part A(O) that conducts ions. Subunit E is a component of the peripheral stalk that links the A(1) with the A(O) part of the A-ATP synthase. We have determined the crystal structure of the entire subunit E (PhE) of the Pyrococcus horikoshii OT3 A-ATP synthase at 3.6 Å resolution. The structure reveals an extended S-shaped N-terminal α-helix with 112.29 Å in length, followed by a globular head group. The S-shaped feature, common in elastic connectors and spacers, would facilitate the storage of transient elastic energy during rotary motion in the enzyme. The structure has been superimposed into the asymmetric peripheral stalks of the three-dimensional reconstruction of the Pyrococcus furiosus enzyme, revealing that the S-shaped subunit PhE fits well into the bent peripheral stalk, whereas the previously solved E subunit structure (3.1 Å resolution) of Thermus thermophilus A-ATP synthase is well accommodated in the density of the straight stator domain. The different features of the two stalk subunits are discussed in light of a novel coupling mechanism in A-ATP synthases proposed to differ from the Wankel engine of F-ATP synthases. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. Reversal of hepatorenal syndrome type 1 with terlipressin plus albumin vs. placebo plus albumin in a pooled analysis of the OT-0401 and REVERSE randomised clinical studies.

    PubMed

    Sanyal, A J; Boyer, T D; Frederick, R T; Wong, F; Rossaro, L; Araya, V; Vargas, H E; Reddy, K R; Pappas, S C; Teuber, P; Escalante, S; Jamil, K

    2017-06-01

    The goal of hepatorenal syndrome type 1 (HRS-1) treatment is to improve renal function. Terlipressin, a synthetic vasopressin analogue, is a systemic vasoconstrictor used for the treatment of HRS-1, where it is available. To compare the efficacy of terlipressin plus albumin vs. placebo plus albumin in patients with HRS-1. Pooled patient-level data from two large phase 3, randomised, placebo-controlled studies were analysed for HRS reversal [serum creatinine (SCr) value ≤133 μmol/L], 90-day survival, need for renal replacement therapy and predictors of HRS reversal. Patients received intravenous terlipressin 1-2 mg every 6 hours plus albumin or placebo plus albumin up to 14 days. The pooled analysis comprised 308 patients (terlipressin: n = 153; placebo: n = 155). HRS reversal was significantly more frequent with terlipressin vs. placebo (27% vs. 14%; P = 0.004). Terlipressin was associated with a more significant improvement in renal function from baseline until end of treatment, with a mean between-group difference in SCr concentration of -53.0 μmol/L (P < 0.0001). Lower SCr, lower mean arterial pressure and lower total bilirubin and absence of known precipitating factors for HRS were independent predictors of HRS reversal and longer survival in terlipressin-treated patients. Terlipressin plus albumin resulted in a significantly higher rate of HRS reversal vs. albumin alone in patients with HRS-1. Terlipressin treatment is associated with improved renal function. (ClinicalTrials.gov identifier: OT-0401, NCT00089570; REVERSE, NCT01143246). © 2017 The Authors. Alimentary Pharmacology and Therapeutics published by John Wiley & Sons Ltd.

  7. Crystal structure of archaeal ribonuclease P protein Ph1771p from Pyrococcus horikoshii OT3: An archaeal homolog of eukaryotic ribonuclease P protein Rpp29

    PubMed Central

    NUMATA, TOMOYUKI; ISHIMATSU, IKUKO; KAKUTA, YOSHIMITSU; TANAKA, ISAO; KIMURA, MAKOTO

    2004-01-01

    Ribonuclease P (RNase P) is the endonuclease responsible for the removal of 5′ leader sequences from tRNA precursors. The crystal structure of an archaeal RNase P protein, Ph1771p (residues 36–127) from hyperthermophilic archaeon Pyrococcus horikoshii OT3 was determined at 2.0 Å resolution by X-ray crystallography. The structure is composed of four helices (α1–α4) and a six-stranded antiparallel β-sheet (β1–β6) with a protruding β-strand (β7) at the C-terminal region. The strand β7 forms an antiparallel β-sheet by interacting with strand β4 in a symmetry-related molecule, suggesting that strands β4 and β7 could be involved in protein-protein interactions with other RNase P proteins. Structural comparison showed that the β-barrel structure of Ph1771p has a topological resemblance to those of Staphylococcus aureus translational regulator Hfq and Haloarcula marismortui ribosomal protein L21E, suggesting that these RNA binding proteins have a common ancestor and then diverged to specifically bind to their cognate RNAs. The structure analysis as well as structural comparison suggested two possible RNA binding sites in Ph1771p, one being a concave surface formed by terminal α-helices (α1–α4) and β-strand β6, where positively charged residues are clustered. A second possible RNA binding site is at a loop region connecting strands β2 and β3, where conserved hydrophilic residues are exposed to the solvent and interact specifically with sulfate ion. These two potential sites for RNA binding are located in close proximity. The crystal structure of Ph1771p provides insight into the structure and function relationships of archaeal and eukaryotic RNase P. PMID:15317976

  8. Ries Bunte Breccia revisited: Indications for the presence of water in Itzing and Otting drill cores and implications for the emplacement process

    NASA Astrophysics Data System (ADS)

    Pietrek, Alexa; Kenkmann, Thomas

    2016-07-01

    We reassessed two drill cores of the Bunte Breccia deposits of the Ries crater, Germany. The objectives of our study were the documentation of evidence for water in the Bunte Breccia, the evaluation of how that water influenced the emplacement processes, and from which preimpact water reservoir it was derived. The Bunte Breccia in both cores can be structured into a basal layer composed mainly of local substrate material, overlain by texturally and compositionally diverse, crater-derived breccia units. The basal layer is composed of the youngest sediments (Tertiary clays and Upper Jurassic limestone) and has a razor-sharp boundary to the upper breccia units, which are composed of older rocks of Upper Jurassic to Upper Triassic age. Sparse material exchange occurred between the basal layer and the rest of the Bunte Breccia. Fluids predominantly came from the Tertiary and the Upper Triassic sandstone formation. In the basal layer, Tertiary clays were subjected to intense, ductile deformation, indicating saturation with water. This suggests that water was mixed into the matrix, creating a fluidized basal layer with a strong shear localization. In the upper units, Upper Triassic sandstones are intensely deformed by granular flow. The texture requires that the rocks were disaggregated into granular sand. Vaporization of pore water probably aided fragmentation of these rocks. In the Otting core, hot suevite (T > 600 °C) covered the Bunte Breccia shortly after its emplacement. Vertically oriented gas escape pipes in suevite partly emanate directly at the contact to the Bunte Breccia. They indicate that the Bunte Breccia contained a substantial amount of water in the upper part that was vaporized and escaped through these vents.

  9. Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids.

    PubMed

    Kawakami, Ryushi; Ohmori, Taketo; Sakuraba, Haruhiko; Ohshima, Toshihisa

    2015-08-01

    To date, there have been few reports analyzing the amino acid requirement for growth of hyperthermophilic archaea. We here found that the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 requires Thr, Leu, Val, Phe, Tyr, Trp, His and Arg in the medium for growth, and shows slow growth in medium lacking Met or Ile. This largely corresponds to the presence, or absence, of genes related to amino acid biosynthesis in its genome, though there are exceptions. The amino acid requirements were dramatically lost by addition of D-isomers of Met, Leu, Val, allo-Ile, Phe, Tyr, Trp and Arg. Tracer analysis using (14)C-labeled D-Trp showed that D-Trp in the medium was used as a protein component in the cells, suggesting the presence of D-amino acid metabolic enzymes. Pyridoxal 5'-phosphate (PLP)-dependent racemase activity toward Met, Leu and Phe was detected in crude extract of P. horikoshii and was enhanced in cells grown in the medium supplemented with D-amino acids, especially D-allo-Ile. The gene encoding the racemase was narrowed down to one open reading frame on the basis of enzyme purification from P. horikoshii cells, and the recombinant enzyme exhibited PLP-dependent racemase activity toward several amino acids, including Met, Leu and Phe, but not Pro, Asp or Glu. This is the first report showing the presence in a hyperthermophilic archaeon of a PLP-dependent amino acid racemase with broad substrate specificity that is likely responsible for utilization of D-amino acids for growth.

  10. An investigation in the MSFC TWT to determine spoiler effects on wing loads and elevon hinge moments utilizing 0.004-scale models (77-0 and 74-OTS) of the shuttle vehicle 5 configuration (IA125)

    NASA Technical Reports Server (NTRS)

    Allen, E. C.

    1976-01-01

    Information is presented for wind tunnel tests (IA125) of a 0.004-scale orbiter, external tank, and solid rocket motor integrated vehicle model (77-0 and 74-OTS) in the MSFC Trisonic Wind Tunnel. These tests were conducted in support of MCR's 1344 and 1346. Data from these tests provide spoiler effects on wing bending/torsion and elevon hinge moments, elevon effectiveness data and the influence of solid plumes from Mach numbers of 0.6 through 2.74 at angles of attack and sideslip from -10 through 10 degrees.

  11. Investigations of the 0.020-scale 88-OTS Integrated Space Shuttle Vehicle Jet-Plume Model in the NASA/Ames Research Center 11 by11-Foot Unitary Plan Wind Tunnel (IA80). Volume 1

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    The results are documented of jet plume effects wind tunnel test of the 0.020-scale 88-OTS launch configuration space shuttle vehicle model in the 11 x 11 foot leg of the NASA/Ames Research Center Unitary Plan Wind Tunnel. This test involved cold gas main propulsion system (MPS) and solid rocket motor (SRB) plume simulations at Mach numbers from 0.6 to 1.4. Integrated vehicle surface pressure distributions, elevon and rudder hinge moments, and wing and vertical tail root bending and torsional moments due to MPS and SRB plume interactions were determined. Nozzle power conditions were controlled per pretest nozzle calibrations. Model angle of attack was varied from -4 deg to +4 deg; model angle of sideslip was varied from -4 deg to +4 deg. Reynolds number was varied for certain test conditions and configurations, with the nominal freestream total pressure being 14.69 psia. Plotted force and pressure data are presented.

  12. Heat transfer tests of an 0.006-scale thin-skin space shuttle thermocouple model (41-OTS) in the Langley Research Center unitary plan wind tunnel at M equals 3.7 (IH16)

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.

    1975-01-01

    The results are presented of supersonic heat transfer tests performed on the .006 scale space shuttle vehicle model (41-OTS) in the Langley Research Center Unitary Plan Wind Tunnel. These tests were conducted to parametrically investigate ascent heating of the integrated vehicle and its components. The tests were conducted at a nominal Mach number of 3.7 and Reynolds numbers per foot of 2 and 5 million. The model configurations investigated were the integrated vehicle and each component alone (i.e. orbiter, tank and SRB). All the configurations were run with and without transition strips and through an angle of attack range of 0 deg to minus 5 deg with the exception of the SRB which was tested through an angle of attack range of minus 5 deg to 90 deg. The heat transfer data were obtained from 223 iron constantan thermocouples attached to stainless steel thin-skin areas of the model.

  13. An experimental determination in Calspan Ludwieg tube of the base environment of the integrated space shuttle vehicle at simulated Mach 4.5 flight conditions (test IH5 of model 19-OTS)

    NASA Technical Reports Server (NTRS)

    Drzewiecki, R. F.; Foust, J. W.

    1976-01-01

    A model test program was conducted to determine heat transfer and pressure distributions in the base region of the space shuttle vehicle during simulated launch trajectory conditions of Mach 4.5 and pressure altitudes between 90,000 and 210,000 feet. Model configurations with and without the solid propellant booster rockets were examined to duplicate pre- and post-staging vehicle geometries. Using short duration flow techniques, a tube wind tunnel provided supersonic flow over the model. Simultaneously, combustion generated exhaust products reproduced the gasdynamic and thermochemical structure of the main vehicle engine plumes. Heat transfer and pressure measurements were made at numerous locations on the base surfaces of the 19-OTS space shuttle model with high response instrumentation. In addition, measurements of base recovery temperature were made indirectly by using dual fine wire and resistance thermometers and by extrapolating heat transfer measurements.

  14. Results of investigations conducted in the LaRC 4-foot unitary plan wind tunnel leg no. 1 using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA94A)

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Aero-loads investigations were conducted on the updated configuration-5 space shuttle launch vehicle at Mach numbers 2.50, 3.50, and 4.50. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed. Various elevon deflection angles were tested, with two different forward orbiter-to-external-tank attach-strut configurations. The entire vehicle model 72-OTS was supported by means of a balance mounted in the orbiter through its base and suspended from an appropriate sting for the specific tunnel.

  15. Results of an investigation of the space shuttle integrated vehicle aerodynamic heating characteristics obtained using the 0.0175-scale model 60-OTS in AEDC tunnel A during tests IH41 and IH41A

    NASA Technical Reports Server (NTRS)

    Cummings, J. W.; Dye, W. H.

    1977-01-01

    A thin skin thermocouple test was conducted to obtain heat-transfer data on the space shuttle integrated vehicle during the ascent phase of the flight profile. The test model was the 0.0175-scale thin skin thermocouple model (60-OTS) of the Rockwell International vehicle 5 configuration. The test was conducted at nominal Mach numbers of 2.5, 3.5, 4.5, and 5.5, and a free stream unit Reynolds number of 5 million per ft. Heat transfer data were obtained for angles of attack of 0, + or - 5, and 10 deg and yaw angles of 0, 3, and 6 deg. The integrated vehicle model was tested with the external tank configured with both a smooth ogive nose and an ogive nose with a spherical nose tip (nipple nose). The remainder of the test was conducted with the external tank installed alone in the tunnel.

  16. Aerodynamic results of a separation effects test on a 0.010-scale model (52-OTS) of the integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A (IA111), volume 1

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1976-01-01

    Graphical data obtained during experimental wind tunnel aerodynamic investigations of a 0.010 scale model (52-OTS) of the integrated space shuttle vehicle was presented. The purpose of this investigation was to obtain data with the solid rocket booster (SRB) in proximity to the orbiter/external tank (O/ET), over a large O/ET initial angle of attack and sideslip range, as well as data on the SRB alone (greatly separated from the O/ET). A captive trajectory system, which supported the SRB, was used with the tunnel primary sector (supporting the O/ET) to obtain grid type separation effects data. One symmetrical SRB model was used interchangeably to obtain right-hand and left-hand SRB data. The entire investigation was conducted at a free-stream Mach number of 4.5 at unit Reynolds number of 3.95 and 5.9 million per foot.

  17. Genesis of rare-metal pegmatites and alkaline apatite-fluorite rocks of Burpala massi, Northern Baikal folded zone

    NASA Astrophysics Data System (ADS)

    Sotnikova, Irina; Vladykin, Nikolai

    2015-04-01

    Burpalinsky rare metal alkaline massif in the Northern Baikal folded zone in southern margin of Siberian Platform, is a of intrusion central type, created 287 Ma covering area of about 250 km2. It is composed of nepheline syenites and pulaskites grading to quartz syenites in the contacts. Veines and dykes are represented by shonkinites, sodalite syenite, leucocratic granophyres, alkali granites and numerous rare metal alkaline syenite pegmatites and two dykes of carbonatites. All rocks except for granites are cut by a large apatite-fluorite dyke rocks with mica and magnetite, which in turn is cut by alaskite granites dyke. The massif has been studied by A.M. Portnov, A.A. Ganzeev et al. (1992) Burpalinsky massif is highly enriched with trace elements, which are concentrated in pegmatite dykes. About 70 rare-metal minerals we found in massif. Zr-silicates: zircon, eudialyte, lovenite, Ti-lovenite, velerite, burpalite, seidozerite, Ca- seidozerite, Rosenbuschite, vlasovite, katapleite, Ca-katapleite, elpidite. Ti- minerals:- sphene, astrophyllite, ramsaite, Mn-neptunite bafertisite, chevkinite, Mn-ilmenite, pirofanite, Sr-perrerit, landauite, rutile, anatase, brookite; TR- minerals - loparite, metaloparite, britolite, rinkolite, melanocerite, bastnesite, parisite, ankilite, monazite, fluocerite, TR-apatite; Nb- minerals - pyrochlore, loparite. Other rare minerals leucophanite, hambergite, pyrochlore, betafite, torite, thorianite, tayniolite, brewsterite, cryolite and others. We have proposed a new scheme massif: shonkinites - nepheline syenites - alkaline syenite - quartz syenites - veined rocks: mariupolites, rare-metal pegmatites, apatite, fluorite rock alyaskite and alkaline granites and carbonatites (Sotnikova, 2009). Apatite-fluorite rocks are found in the central part of massif. This is a large vein body of 2 km length and a 20 m width cutting prevailing pulaskites. Previously, these rocks were regarded as hydrothermal low-temperature phase. New geological and thermobarometric evidence suggests that apatite-fluorite rocks were formed from the residual fluid-melt, separated after crystallization of rare-metal pegmatites. Petrochemical and geochemical data Burpalinsky are in accord of general trend of crystal differentiation of alkaline magma containing small concentrations of CO2 and higher P2O5 and F, which accumulated significantly separated from the pegmatite melts. In some pegmatites fluorite with rare-metal minerals (flyuocerit etc) are separating in schlieren. Apatite-fluorite rocks are cut by leucogranite dyke, having genetic connection with rare-metal pegmatites. Late granitic phases has its own association of rare-metal minerals described by A.A. Ganzeev (1972). Thermobarometric geochemical study of apatite-fluorite rocks Burpala massif found a large number of primary fluid inclusions (15-50 micrometers). Thermal and cryometric research of 60 individual fluid inclusions in fluorite showed the domination of Na, Ca, Mg chlorides and high temperatures salt inclusions in fluorites (above 550C) and melt inclusions in apatites (800C). Apatite-fluorite rocks in massif are similar to foskorites in carbonatite complexes, with similar high Ca content, but instead fluorite, together with other "foskoritovymi" minerals - apatite, magnetite, mica, and pyroxene were formed instead for calcite. Isotopic studies (Sr-Nd) indicate the mantle source of primary magma Burpala massif close to EM-2, which is characteristic of alkaline intrusions in the folded belts (Vladykin 2009). RBRF grant 14-45-04057

  18. Aleppo pine afforestation in the Massis del Caroig, Eastern Spain. The impact on soil water repellency and infiltration rates.

    NASA Astrophysics Data System (ADS)

    Cerdà, Artemi; González Pelayo, Óscar; Jordán, Antonio; Mataix Solera, Jorge; Úbeda, Xavier

    2015-04-01

    Paloma Hueso and co-workers (2014; 2015) researched the impact of soil treatment on soil erosion and organic matter recovery in Mediterranean types ecosystems and they demonstrated that the surface wash and the soil quality is determined by the soil management. Afforestation and proper management with fertilizers, mulches and vegetation recovery, are common strategies to flight against soil erosion in Mediterranean type ecosystems García Orenes et al., 2010; Barbera et al., 2012; García Orenes et al., 2012; Mekuria and Aynekulu, 2013; Jiménez et al., 2015; Tengberg et al., 2015; Tesfaye et al., 2015). However, Hueso et al., (2014; 2015) did not paid attention to the impact that water repellency can trigger in the runoff generation and water repellency when soils increase the organic matter. In Eastern Spain, afforestation with Aleppo Pine (Pinus halepensis Mill.) was very popular during the XX century, although little is know about his impact on soil hydrology. Many of the impacts of afforestation were found positive (García et al., 2000; Maestre et al., 2003; Bellot et al., 2004; Maestre and Cortina, 2004; Chirino et al., 2006; Querejeta et al., 2008; ). This research shows the impact of Pinus halepensis Mill. on soil water repellency, in comparison to the natural scrubland and the cover of Quercus ilex. Within the El Teularet-Sierra de Enguera Experimental Station five types of vegetation covers were selected: Pinus halepensis, Quercus Ilex, Quercus coccifera, Rosmarinus officinalis, Thymus vulgaris and Brachypodium retusum. The Water Drop Penetration Time method (Cerda and Doerr; 2007; 2008) was applied. A hundred drops were applied at the soil surface, 1, 2, 5 and 10 cm depth 5 times along the year 2013 under different soil moisture content. The results show that the water repllency of the soils is: Pinus Pinus halepensis > Quercus coccifera > Rosmarinus officinalis > Quercus ilex > Thymus vulgaris > Brachypodium retusum. This is related to the higher organic matter content and the production of water repellent substances by the vegetation. Acknowledgements The research projects GL2008-02879/BTE, LEDDRA 243857 and RECARE supported this research. References Barbera, V., Poma, I., Gristina, L., Novara, A., Egli, M. 2012. Long-term cropping systems and tillage management effects on soil organic carbon stock and steady state level of C sequestration rates in a semiarid environment. Land Degradation & Development, 23: 82- 91. Bellot, J., Maestre, F. T., Chirino, E., Hernández, N., de Urbina, J. O. 2004. Afforestation with< i> Pinus halepensis reduces native shrub performance in a Mediterranean semiarid area. Acta oecologica, 25(1), 7-15. Cerdà, A. & Doerr, S.H. 2007. Soil wettability, runoff and erodibility of major dry-Mediterranean land use types on calcareous soils. Hydrological Processes, 21, 2325-2336. (doi: 10.1016/j.catena.2008.03.010) Cerdà, A. y Doerr, S.H. 2008. The effect of ash and needle cover on surface runoff and erosion in the immediate post-fire period. Catena, 74 , 256- 263. doi:10.1016/S0341-8162(02)00027-9 Chirino, E., Bonet, A., Bellot, J., Sánchez, J. R. 2006. Effects of 30-year-old Aleppo pine plantations on runoff, soil erosion, and plant diversity in a semi-arid landscape in south eastern Spain. Catena, 65(1), 19-29. García-Orenes, F., Cerdà, A., Mataix-Solera, J., Guerrero, C., Bodí, M.B., Arcenegui, V., Zornoza, R. & Sempere, J.G. 2009. Effects of agricultural management on surface soil properties and soil-water losses in eastern Spain. Soil and Tillage Research 106, 117-123. 10.1016/j.still.2009.06.002 García-Orenes, F., Guerrero, C., Roldán, A.,Mataix-Solera, J., Cerdà, A., Campoy, M., Zornoza, R., Bárcenas, G., Caravaca. F. 2010. Soil microbial biomass and activity under different agricultural management systems in a semiarid Mediterranean agroecosystem. Soil and Tillage Research 109, 110-115. 10.1016/j.still.2010.05.005. García-Orenes, F., Roldán, A., Mataix-Solera, J., Cerdà, A., Campoy, M., Arcenegui, V., Caravaca, F. 2012. Soil structural stability and erosion rates influenced by agricultural management practices in a semi-arid Mediterranean agro-ecosystem. Soil Use and Management 28, 571-579. DOI: 10.1111/j.1475-2743.2012.00451.x García, C., Hernández, T., Roldán, A., Albaladejo, J., Castillo, V. 2000. Organic amendment and mycorrhizal inoculation as a practice in afforestation of soils with< i> Pinus halepensis Miller: effect on their microbial activity. Soil Biology and Biochemistry, 32(8), 1173-1181. Hueso-González, P., Ruiz-Sinoga, J. D., Martínez-Murillo, J. F., Lavee, H. 2015. Overland flow generation mechanisms affected by topsoil treatment: Application to soil conservation. Geomorphology, 228, 796-804. Hueso-González, P., Martínez-Murillo, J. F., & Ruiz-Sinoga, J. D. (2014). The impact of organic amendments on forest soil properties under Mediterranean climatic conditions. Land Degradation & Development, 25(6), 604-612. Jiménez, M. N., Fernández-Ondoño, E., Ripoll, M. Á., Castro-Rodríguez, J., Huntsinger, L., & Navarro, F. B. (2015). Stones and organic mulches improve the Quercus ilex L. afforestation success under Mediterranean climatic conditions. Land Degradation & Development.| DOI: 10.1002/ldr.2250 Maestre, F. T., & Cortina, J. (2004). Are< i> Pinus halepensis plantations useful as a restoration tool in semiarid Mediterranean areas?. Forest Ecology and Management, 198(1), 303-317. Maestre, F. T., Cortina, J., Bautista, S., & Bellot, J. 2003. Does< i> Pinus halepensis facilitate the establishment of shrubs in Mediterranean semi-arid afforestations?. Forest Ecology and Management, 176(1), 147-160. Mekuria, W., Aynekulu, E. 2013. Exclosure land management for restoration of the soils in degrade communal grazing lands in Northern Ethiopia. Land Degradation & Development, 24: 528- 538. DOI 10.1002/ldr.1146 Querejeta, J. I., Barberá, G. G., Granados, A., & Castillo, V. M. 2008. Afforestation method affects the isotopic composition of planted< i> Pinus halepensis in a semiarid region of Spain. Forest Ecology and Management, 254(1), 56-64. Tengberg, A., Radstake, F., Zhang, K., Dunn, B. 2015. Scaling up of sustainable land management in the Western People's republic of China: Evaluation of a 10 year partnership. Land Degradation & Development. | DOI: 10.1002/ldr.2270 Tesfaye, M. A., Bravo-Oviedo, A., Bravo, F., Kidane, B., Bekele, K., Sertse, D. 2015. Selection of tree species and soil management for simultaneous fuelwood production and soil rehabilitation in the Ethiopian Central Highlands.. Land Degradation & Development. DOI: 10.1002/ldr.2268

  19. Results of aeroheating DFI and ET design-data test on a 0.0175-scale model 60-OTS conducted in the Von Karman Gas Dynamics Facility (VKF) 40" supersonic and the 50" hypersonic wind tunnels A and C

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Leef, C. R.

    1985-01-01

    The 0.0175-scale thin-skin thermocouple Model 60-OTS was tested in the von Karman Gas Facility 40-inch continuous flow supersonic tunnel A and the VKF Tunnel C. Testing was conducted at Mach numbers 2.25 to 4.0, and Reynolds numbers from 0.4 x 10 to the 6th power/ft to 6.6 x 10 to the 6th power/ft. Angle of attack range was from -5.0 to +5.0 degrees, and angle of sideslip range was from -6 to +6 degrees. The primary objective of this test was to provide a valid base for the external tank (ET) and solid rocket booster (SRB) heating prediction methodology for ascent flight by taking heating data at Development Flight Instrumentation (DFI) locations for flight conditions simulating STS-1 through -4. A second objective was to obtain additional aeroheating data to support potential reduction of the thermal protection system (TPS) on the ET. The third phase of the test was funded and conducted by NASA/MSFC for the purpose of establishing confidence in the data base from the lower temperature tunnel A.

  20. Aerodynamic results of a separation effects test on a 0.01-scale model (52-OTS) of integrated SSV in the AEDC/VKF 40-by-40 inch supersonic wind tunnel A, volume 1

    NASA Technical Reports Server (NTRS)

    Campbell, J. H., II

    1975-01-01

    Experimental aerodynamic investigations were conducted, during the period July 18-19, 1974, in the AEDC/VKF Tunnel A facility on a 0.01-scale model (52-OTS) of the integrated space shuttle vehicle, including only one SRB. The purpose of the investigation was to obtain data for close-in proximity (SRB to orbiter/tank) effects with the orbiter/tank combination at relatively high alpha and beta attitudes, and with the SRB separation motors off. The AEDC Captive Trajectory System (CTS), which supported the SRB, was used in conjunction with the tunnel primary sector (supporting the orbiter/tank) to obtain grid type separation effects data. The one symmetrical SRB model was used interchangeably to obtain both right-hand and left-hand SRB data. Free-stream data were also obtained for the orbiter/tank and for the SRB. This data was used to provide baselines for proximity effects. The entire investigation was conducted at a free-stream Mach number of 4.5 with unit Reynolds number ranging from 4.0 to 6.5 million per foot.

  1. A new report of Nocardiopsis valliformis strain OT1 from alkaline Lonar crater of India and its use in synthesis of silver nanoparticles with special reference to evaluation of antibacterial activity and cytotoxicity.

    PubMed

    Rathod, Dnyaneshwar; Golinska, Patrycja; Wypij, Magdalena; Dahm, Hanna; Rai, Mahendra

    2016-10-01

    The authors report the biological synthesis of silver nanoparticles (AgNPs) by alkaliphilic actinobacterium Nocardiopsis valliformis OT1 strain isolated for the first time from Lonar crater, India. The primary detection of silver NPs formation was made by colour change from colourless to dark brown and confirmed by UV-Vis spectrum of AgNPs at 423 nm, specific for AgNPs. Further, AgNPs were characterized by nanoparticle tracking analysis, Zeta sizer, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) analyses. FTIR analysis showed the presence of proteins as capping agent. TEM analysis revealed the formation of spherical and polydispersed AgNPs within the size range of 5-50 nm. The antimicrobial activity of silver nanoparticles against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was evaluated. The AgNPs showed the maximum antibacterial activity against B. subtilis (Gram positive) and the minimum against E. coli (Gram negative). The minimal inhibitory concentration values of AgNPs for the tested bacteria were found to be in the range of 30-80 µg/mL. The AgNPs demonstrated higher antibacterial activity against all the bacteria tested as compared with the commercially available antibiotics. The cytotoxicity of biosynthesized AgNPs against in vitro human cervical cancer cell line (HeLa) demonstrated a dose-response activity. The IC50 value was found to be 100 µg/mL of AgNPs against cancer HeLa cell line.

  2. Exploring Cultural Competence amongst OT Students.

    PubMed

    Govender, Pragashnie; Mpanza, December M; Carey, Tarryn; Jiyane, Kwenzile; Andrews, Bicolé; Mashele, Sam

    2017-01-01

    Occupational therapy relies primarily on communication between the therapist and client for effective intervention. Adequate communication may be influenced by language and cultural differences between the therapist and client. Cultural competence in relation to language and culture is thus a vital part in practice. Limited research exists on cultural competence in occupational therapy students. This study thus aimed to explore the cultural competence of final year students and their perceptions of their own cultural competence, with respect to language and culture in their practice as students. An explorative qualitative study design was utilised with a nonprobability purposeful sample of 21 final year undergraduate students at a tertiary institute in South Africa. Three focus groups were conducted, comprising between 6 and 8 students in each group. Thematic analysis using inductive reasoning was undertaken in order to analyse the students' experiences and understanding of cultural competence. Findings of the study suggest that cultural competence, in relation to language and culture, influences the occupational therapy intervention process. It was shown to both positively and negatively influence intervention through supporting or hindering rapport building, client centeredness, and effective intervention.

  3. Exploring Cultural Competence amongst OT Students

    PubMed Central

    Mpanza, December M.; Carey, Tarryn; Jiyane, Kwenzile; Andrews, Bicolé; Mashele, Sam

    2017-01-01

    Occupational therapy relies primarily on communication between the therapist and client for effective intervention. Adequate communication may be influenced by language and cultural differences between the therapist and client. Cultural competence in relation to language and culture is thus a vital part in practice. Limited research exists on cultural competence in occupational therapy students. This study thus aimed to explore the cultural competence of final year students and their perceptions of their own cultural competence, with respect to language and culture in their practice as students. An explorative qualitative study design was utilised with a nonprobability purposeful sample of 21 final year undergraduate students at a tertiary institute in South Africa. Three focus groups were conducted, comprising between 6 and 8 students in each group. Thematic analysis using inductive reasoning was undertaken in order to analyse the students' experiences and understanding of cultural competence. Findings of the study suggest that cultural competence, in relation to language and culture, influences the occupational therapy intervention process. It was shown to both positively and negatively influence intervention through supporting or hindering rapport building, client centeredness, and effective intervention. PMID:29097961

  4. Resistant Hypertension On Treatment (ResHypOT): sequential nephron blockade compared to dual blockade of the renin-angiotensin-aldosterone system plus bisoprolol in the treatment of resistant arterial hypertension - study protocol for a randomized controlled trial.

    PubMed

    Cestário, Elizabeth do Espirito Santo; Fernandes, Letícia Aparecida Barufi; Giollo-Júnior, Luiz Tadeu; Uyemura, Jéssica Rodrigues Roma; Matarucco, Camila Suemi Sato; Landim, Manoel Idelfonso Paz; Cosenso-Martin, Luciana Neves; Tácito, Lúcia Helena Bonalume; Moreno, Heitor; Vilela-Martin, José Fernando; Yugar-Toledo, Juan Carlos

    2018-02-12

    resistant hypertension (RH) treatment has increased. Thus, identifying the contribution of intravascular volume and serum renin in maintaining BP levels could help tailor more effective hypertension treatment, whether by acting on the control of intravascular volume or sodium balance, or by acting on the effects of the RAAS on the kidney. Sequential Nephron Blockade vs. Dual Blockade Renin-angiotensin System + Bisoprolol in Resistant Arterial Hypertension (ResHypOT). ClinicalTrials.gov, ID: NCT02832973 . Registered on 14 July 2016. First received: 12 June 2016. Last updated: 18 July 2016.

  5. Results of a M = 5.3 heat transfer test of the integrated vehicle using phase-change paint techniques on the 0.0175-scale model 56-OTS in the NASA/Ames Research Center 3.5-foot hypersonic wind tunnel

    NASA Technical Reports Server (NTRS)

    Marroquin, J.

    1985-01-01

    An experimental investigation was performed in the NASA/Ames Research Center 3.5-foot Hypersonic Wind Tunnel to obtain supersonic heat-distribution data in areas between the orbiter and external tank using phase-change paint techniques. The tests used Novamide SSV Model 56-OTS in the first and second-stage ascent configurations. Data were obtained at a nominal Mach number of 5.3 and a Reynolds number per foot of 5 x 10 to the 6th power with angles of attack of 0 deg, +/- 5 deg, and sideslip angles of 0 deg and +/- 5 deg.

  6. Identity of Innovative Multifunctional Material Manufacturing Business in Latvia / Inovatīvu Daudzfunkcionālo Materiālu Ražotāju Identitāte Latvijā

    NASA Astrophysics Data System (ADS)

    Geipele, I.; Staube, T.; Ciemleja, G.; Zeltins, N.; Ekmanis, Yu.

    2015-08-01

    The publication comprises the results from the practical scientific investigation to define the profile and distinctions of the Latvian innovative multifunctional material manufacturing industry. The research is carried out by a holistic approach, including expert interviews, qualitative analysis of the official register data, practical survey, and financial data analysis. The paper seeks to give the detailed data on a technological profile of the mentioned representative companies, if there is a synergy or tough competition in the Latvian market. The current research is topical, because it is unique and does not have analogues in Latvia, and the research is timely due to correspondence with recently stated Research and Innovation Strategies for Smart Specialisation in Latvia. The main findings are associated with recognition of the factors that make impact on commercialisation of the finished goods, obtained financial results and planned directions of development of the respondent companies. Publikācija sniedz veiktā zinātniski praktiskā pētījuma rezultātus, kura ietvaros tika noteikts Latvijas inovatīvu daudzfunkcionālo materiālu ražotāju darbības profils un identitāte. Pētījumā tika izmantota kompleksās analīzes metode, kas ietvēra ekspertu intervijas, uzņēmumu oficiāli reģistrēto datu kvalitatīvo analīzi, zinātniski praktisko aptauju, kā arī pētāmo uzņēmumu finanšu darbības rezultātu analīzi. Zinātniskais darbs sniedz izsmeļošu informāciju par mērķa uzņēmumu tehnoloģisko profilu un pēta, vai Latvijas mērķa ražošanas nozarē ir sinerģijas vai sīvās konkurences pazīmes. Šim zinātniskajam pētījumam ir noteikta aktualitāte saskaņā ar tā unikalitāti Latvijas valsts mērogā un atbilstība Viedās specializācijas stratēģijai. Pētījuma galvenie secinājumi ir saistīti ar uzņēmumu produkcijas komercializācijas ietekmējošo faktoru apzināšanu, sasniegtajiem finansiālajiem rezultātiem un pl

  7. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... of legal or supervisory issues; (C) The board of directors and executive officers of the holding... the association; (D) The acquisition raises no significant issues of law or policy under then current... frames: (1) On the business day prior to the date of consummation of the acquisition, the chief financial...

  8. Complete genome sequence of Haliscomenobacter hydrossis type strain (OT)

    SciTech Connect

    Daligault, Hajnalka E.; Lapidus, Alla L.; Zeytun, Ahmet

    2011-01-01

    Haliscomenobacter hydrossis van Veen et al. 1973 is the type species of the genus Halisco- menobacter, which belongs to order 'Sphingobacteriales'. The species is of interest because of its isolated phylogenetic location in the tree of life, especially the so far genomically un- charted part of it, and because the organism grows in a thin, hardly visible hyaline sheath. Members of the species were isolated from fresh water of lakes and from ditch water. The genome of H. hydrossis is the first completed genome sequence reported from a member of the family 'Saprospiraceae'. The 8,771,651 bp long genome with itsmore » three plasmids of 92 kbp, 144 kbp and 164 kbp length contains 6,848 protein-coding and 60 RNA genes, and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.« less

  9. NYPIRG Petion to Object ot Kings Plaza Title V Permit

    EPA Pesticide Factsheets

    This document may be of assistance in applying the Title V air operating permit regulations. This document is part of the Title V Petition Database available at www2.epa.gov/title-v-operating-permits/title-v-petition-database. Some documents in the database are a scanned or retyped version of a paper photocopy of the original. Although we have taken considerable effort to quality assure the documents, some may contain typographical errors. Contact the office that issued the document if you need a copy of the original.

  10. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your last...

  11. An OT Account of Laryngealization in Cuzco Quechua.

    ERIC Educational Resources Information Center

    Parker, Steve

    Classical phonemic accounts of Cuzco (Peru) Quechua posit three distinct types of stops: plain, aspirated, and glottalized. A later analysis argued instead for a root-level feature of laryngealization governed by a small number of formal mechanisms. This latter analysis is taken one step further, showing that even greater explanatory power may be…

  12. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your last...

  13. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your last...

  14. 12 CFR 555.310 - How do I notify OTS?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...) Describe the transactional web site. (2) Indicate the date the transactional web site will become operational. (3) List a contact familiar with the deployment, operation, and security of the transactional web site. (b) Transition provision. If you established a transactional web site after the date of your last...

  15. OTS Selective Bibliography. High Temperature Metallurgy and Heat Resistant Alloys

    DTIC Science & Technology

    1961-02-01

    toLibraryof Congress, Photoduplicarion Service, Publication Board offices Project , Washington 25, D. C. Make check or money order payable To Order From...EXTRAPOLATION OF CREEP AND STRESS-RUPTURE DATA, by Manson and National Research Corp., Cambridge, Mass. Haferd. 1953 . 49p. INVESTIGATION OF NICKEL BASE...ALLOYS, a bibliography by Hood. Order from LC mi$2.40 ph$3.30 PB 114262 1953 . 105p. Order from LC mi$4.50 ph$13.75 PB 112022 INVESTIGATION OF NICKEL

  16. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... crime or racketeering; (B) Violation of securities or commodities laws or regulations; (C) Violation of... projecting activities which are inconsistent with economical home financing. [54 FR 49690, Nov. 30, 1989, as...

  17. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... crime or racketeering; (B) Violation of securities or commodities laws or regulations; (C) Violation of... projecting activities which are inconsistent with economical home financing. [54 FR 49690, Nov. 30, 1989, as...

  18. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... crime or racketeering; (B) Violation of securities or commodities laws or regulations; (C) Violation of... projecting activities which are inconsistent with economical home financing. [54 FR 49690, Nov. 30, 1989, as...

  19. 12 CFR 574.7 - Determination by the OTS.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... crime or racketeering; (B) Violation of securities or commodities laws or regulations; (C) Violation of... projecting activities which are inconsistent with economical home financing. [54 FR 49690, Nov. 30, 1989, as...

  20. 12 CFR 510.5 - Release of unpublished OTS information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... testimony or informal interview, that was acquired in the course of performing official duties or because of... wish to question the witness beyond the authorized scope should request expanded authorization pursuant...

  1. Report: Ozone Transport Commission Incurred Costs Under EPA Assistance Agreements XA98379901, OT83098301, XA97318101, and OT83264901

    EPA Pesticide Factsheets

    Report #2007-4-00068, July 31, 2007. We questioned $2,723,706 of the $9,042,706 in reported outlays because the recipient claimed unallowable outlays for contractual services, indirect costs, and in-kind costs.

  2. (/sup 3/H)-(Thr4,Gly7)OT: a highly selective ligand for central and peripheral OT receptors

    SciTech Connect

    Elands, J.; Barberis, C.; Jard, S.

    1988-01-01

    Oxytocin receptors in rat hippocampal synaptic plasma membranes were compared with mammary gland and uterine oxytocin receptors. For this purpose, a highly specific oxytocic agonist (Thr4,Gly7)oxytocin was tritiated. We demonstrated that this ligand labels oxytocin receptors selectively. Scatchard analyses revealed a high affinity for all the oxytocin receptors investigated, with equilibrium dissociation constants between 1.0 and 2.0 nM. Binding appeared to take place at a single population of receptor sites. Competition experiments confirmed the high affinity of arginine vasopressin for hippocampal oxytocin receptors but also revealed that mammary gland and uterine oxytocin receptors do not discriminate more efficiently between oxytocinmore » and arginine vasopressin. This lack in specificity is not affected by applying different concentrations of Mg ions.« less

  3. OT2_jhewitt_2: Understanding Shock Oxygen Chemistry in Interacting Supernova Remnants

    NASA Astrophysics Data System (ADS)

    Hewitt, J.

    2011-09-01

    Supernova remnants interacting with dense moelcular clouds provide astrochemical laboratories to study heating and cooling of the dense ISM, shock chemistry, destruction and sputtering of dust, and the reformation of molecules. Water is expected to be a major coolant for shocks into dense gas, yet the number of remnants in which IR lines of hydroxyl and water are detected is very limited. We propose Herschel PACS, SPIRE and HIFI observations of three remnants with particularly high shocked gas densities, high dust and IR line luinosities, and extreme ionization environments. The scientific objectives of this proposal are: (1) to determine the abundance and excitation of oxygen-bearing molecules, and (2) to study the effects of variable ionization sources on oxygen chemistry in dense molecular gas shocked by powerful supernova remnant blast waves.

  4. OT2_dardila_2: PACS Photometry of Transiting-Planet Systems with Warm Debris Disks

    NASA Astrophysics Data System (ADS)

    Ardila, D.

    2011-09-01

    Dust in debris disks is produced by colliding or evaporating planetesimals, the remnant of the planet formation process. Warm dust disks, known by their emission at =<24 mic, are rare (4% of FGK main-sequence stars), and specially interesting because they trace material in the region likely to host terrestrial planets, where the dust has very short dynamical lifetimes. Dust in this region comes from very recent asteroidal collisions, migrating Kuiper Belt planetesimals, or migrating dust. NASA's Kepler mission has just released a list of 1235 candidate transiting planets, and in parallel, the Wide-Field Infrared Survey Explorer (WISE) has just completed a sensitive all-sky mapping in the 3.4, 4.6, 12, and 22 micron bands. By cross-identifying the WISE sources with Kepler candidates as well as with other transiting planetary systems we have identified 21 transiting planet hosts with previously unknown warm debris disks. We propose Herschel/PACS 100 and 160 micron photometry of this sample, to determine whether the warm dust in these systems represents stochastic outbursts of local dust production, or simply the Wien side of emission from a cold outer dust belt. These data will allow us to put constraints in the dust temperature and infrared luminosity of these systems, allowing them to be understood in the context of other debris disks and disk evolution theory. This program represents a unique opportunity to exploit the synergy between three great space facilities: Herschel, Kepler, and WISE. The transiting planet sample hosts will remain among the most studied group of stars for the years to come, and our knowledge of their planetary architecture will remain incomplete if we do not understand the characteristics of their debris disks.

  5. GIOTTO's antenna de-spin mechanism: Ots lubrication and thermal vacuum performance

    NASA Technical Reports Server (NTRS)

    Todd, M. J.; Parker, K.

    1987-01-01

    Except in the near Earth phase of GIOTTO's mission to Comet Halley, the HGA (high gain antenna) on board GIOTTO was the only designed means of up/down communications. The spacecraft spin stabilization required that the HGA be despun at the same rotational rate of nominally 15 rpm in order to keep the HGA pointing accurately to a Earth. A dual servomotor despin mechanism was designed and built by SEP of France for this purpose. The expected thermal environment suggested that dry lubrication was preferable to wet for the ball bearings but there existed no relevant data on the torque noise spectrum of candidate solid lubricants. Therefore ad hoc torque noise tests were run with two solid lubricants: ion plated lead film plus lead bronze cage (retainer) and a PTFE composite cage only. The lead lubrication showed the better spectrum up to the mission lifetime point so it was selected for continued test over some 20 times the Halley mission life, with periodic torque spectrum monitoring. The spectrum remained well within the pointing error budget over the 100 million revolutions covered.

  6. OT1_nschneid_1: Pillars of creation: physical origin and connection to star formation

    NASA Astrophysics Data System (ADS)

    Schneider, N.

    2010-07-01

    Herschel SPIRE/PACS photometry observations performed within the HOBYS (Herschel imaging survey of OB Young Stellar objects) key program have revealed a wealth of interesting structures in high-mass star forming regions. The most spectacular of those are 'pillars' and 'globules'. These features -- partly known from Hubble Space telescope or Spitzer images -- are formed due to photoevaporation at the interface between a molecular cloud and an HII region, and are thus intimately linked to high-mass star formation. The process of how these pillars are created, and under which conditions low- or high-mass stars form within them, are not yet clear. Classical approaches (e.g. Rayleigh-Taylor) can not explain pillar formation, so we have embarked upon a dedicated project to fully simulate pillars and globules using the (magneto)-hydrodynamic code HERACLES that comprises gravity and ionization. The model is intended to be coupled with a radiative transfer photon dominated region code (KOSMA-tau). We propose here to make use of the Herschel spectroscopy capacities to map/make single pointings, in a number of atomic and molecular lines, of selected pillars and globules in three different regions (Rosette, Cygnus, M16), spanning a large range in UV intensity and density. We intend to observe the important cooling lines of [CII] at 158 micron and [OI] at 63 and 145 micron with PACS, the [CI] finestructure lines at 370 and 609 micron and the mid-to high-J CO and HCO+ ladder with the SPIRE FTS. Spectrally resolved [CII] mapping with HIFI is also required to derive the velocity information. These observations will be compared to the large existing complementary data set for each source, to study the physics of pillars and will additionally serve as input for the models, to ultimately explain pillar formation and star formation within them.

  7. 78 FR 76721 - Removal of Transferred OTS Regulations Regarding Recordkeeping and Confirmation Requirements for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... in the preamble, the Board of Directors of the Federal Deposit Insurance Corporation revises part 344...; and (2) If the FDIC-supervised institution is to receive remuneration from the customer or any other source in connection with the transaction, a statement of the source and amount of any remuneration to be...

  8. 78 FR 54403 - Removal of Transferred OTS Regulations Regarding Recordkeeping and Confirmation Requirements for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-04

    ... enforced by the FDIC and the OCC, respectively. On June 14, 2011, the FDIC's Board of Directors approved a..., 2011, operating pursuant to this authority, the FDIC's Board of Directors reissued and redesignated... Directors to waive any provision of the part for good cause shown.\\10\\ The other Agencies also amended their...

  9. OT1_mhuang01_1: GRB Afterglow Photometry with Herschel Infrared Cameras

    NASA Astrophysics Data System (ADS)

    Huang, M.

    2010-07-01

    GRB Afterglow Photometry with Herschel Infrared Cameras (GRAPHICS) Gamma-ray bursts (GRBs) are the most luminous explosions in the universe. It has been difficult to obtain a full spectral picture of the phenomena in the short period when GRBs become ``alive'', i.e. when they generate bursts in Gamma-ray and produce afterglows in other wavelengths. Between NIR (12micron) and submillimeter (850micron) there lies nearly two orders of magnitude of spectral range where GRB afterglows have never been detected. Herschel is unique in its cutting edge sensitivity, efficiency, and readiness in FIR observations, and is capable of detecting GRB afterglows. Observing GRB afterglows with Herschel would greatly enrich our understanding of GRB physics and conditions of the Universe in early epochs. We propose Target of Opportunity studies using the SPIRE and PACS instruments of Herschel to observe 3 bright GRB afterglows, each within a few hours to a few tens of days after burst. We will make follow-up observations after the initial one to photometrically measure GRB light curves and IR SEDs. We will make ground optical observations to compliment Herschel data, and have the the GRB community informed. Observing the forward shock peak in the FIR light curve and compare it (both the flux and time) with those in the optical and radio bands would give a unambiguous test to the fireball model, and offer a direct measurement of the density profile of the circumburst material. Catching the short-lived reverse shock emission and measure its magnitude would lead to constraints on some important parameters of the GRB ejecta and address the unknown composition of GRBs, baryonic vs. magnetic.

  10. A Guide to Reasonable Accommodation for OT Practitioners with Disabilities: Fieldwork to Employment.

    ERIC Educational Resources Information Center

    Wells, Shirley A., Comp.; Hanebrink, Sandy, Comp.

    This handbook provides information to occupational therapy clinicians and students with disabilities, fieldwork educators, and employers on issues of accommodation, disclosure, rights, responsibilities, and the Americans with Disabilities Act (ADA). It begins with a discussion of the disability rights movement, and employment and people with…

  11. OT2_tvelusam_4: Probing Galactic Spiral Arm Tangencies with [CII

    NASA Astrophysics Data System (ADS)

    Velusamy, T.

    2011-09-01

    We propose to use the unique viewing geometry of the Galactic spiral arm tangents , which provide an ideal environment for studying the effects of density waves on spiral structure. We propose a well-sampled map of the[C II] 1.9 THz line emission along a 15-degree longitude region across the Norma-3kpc arm tangential, which includes the edge of the Perseus Arm. The COBE-FIRAS instrument observed the strongest [C II] and [N II] emission along these spiral arm tangencies.. The Herschel Open Time Key Project Galactic Observations of Terahertz C+ (GOT C+), also detects the strongest [CII] emission near these spiral arm tangential directions in its sparsely sampled HIFI survey of [CII] in the Galactic plane survey. The [C II] 158-micron line is the strongest infrared line emitted by the ISM and is an excellent tracer and probe of both the diffuse gases in the cold neutral medium (CNM) and the warm ionized medium (WIM). Furthermore, as demonstrated in the GOTC+ results, [C II] is an efficient tracer of the dark H2 gas in the ISM that is not traced by CO or HI observations. Thus, taking advantage of the long path lengths through the spiral arm across the tangencies, we can use the [C II] emission to trace and characterize the diffuse atomic and ionized gas as well as the diffuse H2 molecular gas in cloud transitions from HI to H2 and C+ to C and CO, throughout the ISM. The main goal of our proposal is to use the well sampled (at arcmin scale) [C II] to study these gas components of the ISM in the spiral-arm, and inter-arm regions, to constrain models of the spiral structure and to understand the influence of spiral density waves on the Galactic gas and the dynamical interaction between the different components. The proposed HIFI observations will consist of OTF 15 degree longitude scans and one 2-degree latitude scan sampled every 40arcsec across the Norma- 3kpc Perseus Spiral tangency.

  12. History of Air Force Operational Test and Evaluation (OT and E) Mission, Organization, and Policy

    DTIC Science & Technology

    1992-12-01

    which also observed that "the decisive factor in the war with Iraq was the air campaign." DoD’s Gulf War report also noted the "extraordinary...and Air Warfare: Training and Testing at Eglin in World War II," in William S. Coker (ed.), The Military Presence on the Gulf Coast (Pensacola, FL...34Test and Evaluation," 12 May 72. 35. Hist of AF/XOOW, Jan-Jim 73. 36. US General Accounting Office, "Statement of Elmer B. Staats , Comptroller

  13. Swift UVOT observations of the SMC nova MASTER OT J010603.18-744715.8

    NASA Astrophysics Data System (ADS)

    Kuin, N. P. M.; Page, K. L.; Williams, S. C.; Darnley, M. J.; Shore, S. N.; Walter, F. M.

    2016-10-01

    Since its discovery at magnitude 10.9 on 2016-10-14.19341 UT (Shumkov et al, ATEL #9621) and spectroscopic confirmation (Williams and Darnley, ATEL #9628) pre-discovery data from the OGLE team (Mroz and Udalski, ATEL #9622, and MASTER (Lipunov et al., ATEL #9631) revealed the progenitor magnitude and pushed back the appearance of the nova to magnitude 8.5 on 2016, Oct 9.808.

  14. 12 CFR 502.15 - How does OTS determine my size component?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... size component is: This amount—Base assessment amount Column C Plus—Marginal rate Column D Of assets... across in that same row, find your base assessment amount in Column C, your marginal rate in Column D... floor. Multiply this number by your Column D marginal rate. Add this number to your Column C base...

  15. OT2_pgolds01_6: Herschel [NII] Observations to Define the Source of [CII] Emission

    NASA Astrophysics Data System (ADS)

    Goldsmith, P.

    2011-09-01

    The 158 micron line of ionized carbon is the strongest single long-wavelength emission feature from the interstellar medium and is the most important coolant of gas in which hydrogen is in atomic form. It is a key determinant of the evolution of these largely atomic regions into denser, cooler molecular clouds in which new stars are formed, and is widely used as a tracer of star formation in the Milky Way and other galaxies. There is, however, an ongoing, serious controversy about the origin of the [CII] emission, which has been asserted to come from the extended low-density warm interstellar medium, but has more generally been associated with the primarily molecular photon dominated regions (PDRs) intimately associated with massive, young stars. We propose a combined HIFI and PACS study of the two far-infrared [NII] fine structure lines in order to resolve the important question of the fraction of CII emission that arises in ionized gas. Specifically, we will (1) utilize the fact that due to its ionization potential NII is found only in HII regions, and with PACS 122 and 205 micron observations, determine electron densities in a sample of such regions in the Galactic plane; (2) utilize available data on radio free-free and H-alpha emission to determine the NII column densities and from this the CII column densities in the HII regions; (3) use the electron densities to determine the fraction of CII emission arising in the ionized interstellar medium. These observations will be carried out at 150 of the positions in the Galactic plane observed in [CII] by the GOT-C+ project. We will also carry out HIFI observations of 10 selected positions in the 205 micron line to determine spectral characteristics of the NII emission line, which with CII, CI, and CO profiles already in hand will serve as a further discriminant among the proposed sources of CII emission.

  16. OT1_mputman_1: ASCII: All Sky observations of Galactic CII

    NASA Astrophysics Data System (ADS)

    Putman, M.

    2010-07-01

    The Milky Way and other galaxies require a significant source of ongoing star formation fuel to explain their star formation histories. A new ubiquitous population of discrete, cold clouds have recently been discovered at the disk-halo interface of our Galaxy that could potentially provide this source of fuel. We propose to observe a small sample of these disk-halo clouds with HIFI to determine if the level of [CII] emission detected suggests they represent the cooling of warm clouds at the interface between the star forming disk and halo. These cooling clouds are predicted by simulations of warm clouds moving into the disk-halo interface region. We target 5 clouds in this proposal for which we have high resolution HI maps and can observe the densest core of the cloud. The results of our observations will also be used to interpret the surprisingly high detections of [CII] for low HI column density clouds in the Galactic Plane by the GOT C+ Key Program by extending the clouds probed to high latitude environments.

  17. The Acquisition of English Lexical Stress by Chinese-Speaking Learners: An OT Account

    ERIC Educational Resources Information Center

    Yuan, Man; Cheng, Wei

    2017-01-01

    Lexical stress is an important contributor to foreign accent as well as intelligibility of second language (L2) speech. The present study intends to find out to what extent Chinese-speaking learners whose native language has less evident stress can acquire English lexical stress. A production test was administered to nine advanced Chinese learners…

  18. Report on the Audit ot the Acquisition of the Tacit Rainbow Anti-Radiation Missile System

    DTIC Science & Technology

    1991-06-24

    This is our final report on the audit of the Tacit Rainbow Anti-Radiation Missile System (Tacit Rainbow) for your information and use. We performed... the audit from March through December 1990. The audit objective was to evaluate the acquisition management of the air-launched Tacit Rainbow system to...contains no recommendations because DoD took action to cancel the Tacit Rainbow system in FY 1992. We are bringing the issues identified during the audit to

  19. Management and Capability ot the Major Range and Test Facility Bases

    DTIC Science & Technology

    1995-07-27

    The audit objective was to evaluate management oversight provided to the test ranges and determine whether management oversight could be streamlined... The audit also evaluated whether program managers were procuring test resources that already existed at the test ranges. Management controls related to the objectives were also evaluated.

  20. Lateral sellar compartment O.T. (cavernous sinus): history, anatomy, terminology.

    PubMed

    Parkinson, D

    1998-08-01

    Claudios Galen (119-199 a.d.) dissected lower animals with parasellar carotid retia bathed in venous blood and transposed his findings to human anatomy. Andreas Vesalius (1514-1564) corrected most of Galen's errors but apparently never looked into this small, extradural compartment, nor, apparently, did Winslow (Exposition Anatomique de la Structure du Corps Humain. London: N. Prevast, 1734), who christened it the "cavernous sinus," (CS) presumably thinking that it would resemble the corpora cavernosa of the penis. Multiple surgical explorations, gross dissections, microscopic views, and vascular casts from early fetuses to an 81 year old have been examined and reviewed. The CS is not a dural sinus nor is it cavernous. The compartment is extradural, and the venous structures contained within consist of a greatly variable plexus of extremely thin-walled veins. The name, CS, is a barrier to the understanding of the structure and function of this extradural anatomical jewel box, which contains fat, myelinated and nonmyelinated nerves, arteries, and a plexus of veins. It is proposed that this name be changed, because it is inaccurate and misleading. The replacement should leave no doubt about its meaning. The lateral sellar compartment is descriptive and accurate. The veins within are a parasellar plexus.

  1. New Frontiers in Optical Science: Terahertz Spectroscopy ot Two Dimensional Systems

    NASA Astrophysics Data System (ADS)

    Lee, Yun-Shik

    2011-10-01

    Terahertz (THz) radiation is electromagnetic radiation whose frequency lies between the microwave and infrared regions of the spectrum. Naturally occurring THz radiation fills up the space of everyday life providing warmth, yet this part of the spectrum remains the least explored region mainly due to the technical difficulties. The technological gap, however, has been rapidly diminishing for the last two decades. The new and exciting frontier of the THz science and technology has encroached on many different disciplines producing a broad range of applications such as medical imaging, sensing of biochemical agents, and ultra-high speed communication. Furthermore, the unique and advanced techniques of the THz spectroscopy have been proved to be a powerful tool to investigate the material properties inaccessible until recently. For example, THz waves strongly interact with electrons and holes in two dimensional systems, in which their dynamics are governed mainly by many-body Coulomb interactions. I will present our experimental studies demonstrating remarkable quantum effects in semiconductor nanostructures and exotic charge carrier dynamics in graphene.

  2. 78 FR 76768 - Removal of Transferred OTS Regulations Regarding Disclosure and Reporting of CRA-Related...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... institutions (``IDIs'') for which the FDIC has been designated the appropriate Federal banking agency. Upon...,'' regulations applicable for all IDIs for which the FDIC has been designated the appropriate Federal banking... issue regulations under the FDI Act and other laws as the ``appropriate Federal banking agency'' or...

  3. ICT and OTs: a model of information and communication technology acceptance and utilisation by occupational therapists.

    PubMed

    Schaper, Louise K; Pervan, Graham P

    2007-06-01

    There is evidence to suggest that health professionals are reluctant to accept and utilise information and communication technologies (ICT) and concern is growing within health informatics research that this is contributing to the lag in adoption and utilisation of ICT across the health sector. Technology acceptance research within the field of information systems has been limited in its application to health and there is a concurrent need to develop and gain empirical support for models of technology acceptance within health and to examine acceptance and utilisation issues amongst health professionals to improve the success of information system implementation in this arena. This paper outlines a project that examines ICT acceptance and utilisation by Australian occupational therapists. It describes the theoretical basis behind the development of a research model and the methodology being employed to empirically validate the model using substantial quantitative, qualitative and longitudinal data. Preliminary results from Phase II of the study are presented. The theoretical significance of this work is that it uses a thoroughly constructed research model, with potentially the largest sample size ever tested, to extend technology acceptance research into the health sector.

  4. The Transition from Spacecraft Development Ot Flight Operation: Human Factor Considerations

    NASA Technical Reports Server (NTRS)

    Basilio, Ralph R.

    2000-01-01

    In the field of aeronautics and astronautics, a paradigm shift has been witnessed by those in academia, research and development, and private industry. Long development life cycles and the budgets to support such programs and projects has given way to aggressive task schedules and leaner resources to draw from all the while challenging assigned individuals to create and produce improved products of processes. however, this "faster, better, cheaper" concept cannot merely be applied to the design, development, and test of complex systems such as earth-orbiting of interplanetary robotic spacecraft. Full advantage is not possible without due consideration and application to mission operations planning and flight operations, Equally as important as the flight system, the mission operations system consisting of qualified personnel, ground hardware and software tools, and verified and validated operational processes, should also be regarded as a complex system requiring personnel to draw upon formal education, training, related experiences, and heuristic reasoning in engineering an effective and efficient system. Unquestionably, qualified personnel are the most important elements of a mission operations system. This paper examines the experiences of the Deep Space I Project, the first in a series of new technology in-flight validation missions sponsored by the United States National Aeronautics and Space Administration (NASA), specifically, in developing a subsystems analysis and technology validation team comprised of former spacecraft development personnel. Human factor considerations are investigated from initial concept/vision formulation; through operational process development; personnel test and training; to initial uplink product development and test support. Emphasis has been placed on challenges and applied or recommended solutions, so as to provide opportunities for future programs and projects to address and disposition potential issues and concerns as early as possible to reap the benefits associated with learning from other's past experiences.

  5. Bulgarski ezik za dobrovoltsi ot Korpusa na mira (Bulgarian Language for Peace Corps Volunteers. Bulgaria).

    ERIC Educational Resources Information Center

    Dimitrova, Aneta; Tomova, Christina; Tasseva, Mila

    Designed for continued language learning beyond the intermediate level, this workbook provides practice and sets of exercises for improving speaking and listening skills in Bulgarian through class participation and discussion, and reading and writing. The book includes grammar explanations, news reports, brief biographies of prominent Bulgarians,…

  6. Bulgarski ezik za dobrovoltsi ot Korpusa na mira (Bulgarian Language for Peace Corps Volunteers. Bulgaria).

    ERIC Educational Resources Information Center

    Dimitrova, Aneta; Tomova, Christina; Tasseva, Mila

    This workbook and class guide for basic-to-intermediate Bulgarian provides a communicative orientation to the language. With its emphasis on social situations and everyday language, the book includes vocabulary, dialogs, discussion topics, and cultural-historical background information about Bulgaria. (CNP)

  7. Human Factors and Training Evaluation of the Improved Tow Vehicle (ITV) During OT/DT 3

    DTIC Science & Technology

    1980-08-01

    and Alexander Nicolini ARI FIELD UNIT AT FORT BENNING, GEORGIAS DTIC SJUL 2 4 1981 U. S. Army Research Institute for the Behavioral and Social Sciences...August 1980 Approved for public release; distribution unlimited. ~’ 4 i MOM,. U. S. ARMY RESEARCH INSTITUTE FOR THE BEHAVIORAL AND SOCIAL SCIENCES A...of reports to: U, S. Army Research institute for the Behavioral and Social Sciences. ATTN: PERI.TP. 5001 Eisenhower Avenue, Aletandria, Virginia 22333

  8. OT2_pbjerkel_1: Herschel observations of the shocked gas in HH54

    NASA Astrophysics Data System (ADS)

    Bjerkeli, P.

    2011-09-01

    A shock that can be studied in detail, using a very limited amount of Herschel time, is the Herbig-Haro object HH54 located in the nearby Chamaeleon II cloud at a distance of 180 pc. The shocked region has an angular extent of roughly 30'' and is not contaminated with emission from other nearby objects. The gas, traced by H2O and CO, emits radiation predominantly in the far-infrared regime. For that reason, this program can only be executed using the instruments aboard the Herschel Space Observatory. We propose spectroscopy of rotational H2O and CO transitions, falling in the wavelength range covered by SPIRE and PACS. These observations will allow us to stratify the shocked region in different physical/kinematical components. We will also improve our understanding of the mechanisms responsible for water production and destruction. Given the relatively large angular extent of the region, we will determine the types of shock responsible for the emission in different positions along the shocked surface. We also propose HIFI observations of selected CO and H2O transitions. A bullet feature has previously been observed in several CO line profiles. Using HIFI, we will constrain the origin and physical properties of the region responsible for this emission.

  9. Ursa Major: ot losya do medvedya %t Ursa Major: from elk to bear

    NASA Astrophysics Data System (ADS)

    Lushnikova, A. V.

    In the article material from various cultural and linguistic sources (Indo-European - Slavic, Indo-Iranian; Uralo-Altaic, Tungus, Ancient Egyptian, Babylonian) is used in order to trace up the chronology of the designation of Ursa Majopr, a constellation which has been playing an important role for people different regions since ancient times. It was used for observing of the visible yearly motion of the Sun, for working out seasonal changes; being a circumpolar and non-hiding behind the horizon it has been perceived as a symbol of immortality, its peculiar positional change during a year lay down in plot of the Uralo-Siberian myths about a cosmic hunt for the Elk, myths about deluge. Data from Uralo-Siberian mythology are analyzed. Designations of Ursa Major in the form of a horned hoofed animal such as elk, deer, cow (Uralo-Altaic, Tungus, Slavic, Indo-Iranian languages; Ancient Greece, Crete, Ancient Egypt) and connected with it (or derived from it) denominations and images of "enclosed space" - "vehicle for travelling and carrying goods" (a wagon, a boat) - "instrument for hunting-fishing, a ritual thing" - "household construction" are taken in consideration. The conclusion is made that the transition of the Ursa Major designation from elk to bear could follow the general tendency to shift from so-called matriarchy to patriarchy, to substitute female deities with male ones, which was reflected "in the rise" of the predatory animal cults (not earlier than II mil. B.C.). To prove this, lexical examples of resemblance and coincidences in designation of homed hoofed (elk, deer) and predatory (bear, wolf) animals should be analyzed. Such a goal-directed investigation of the chronology of Ursa Major designations has never been carried out.

  10. I Have Students with Physical and Motor Problems: How Can an APE, OT, or PT Help?

    ERIC Educational Resources Information Center

    Silliman-French, Lisa; Candler, Catherine; French, Ron; Hamilton, Merry Lynne

    2007-01-01

    Majority of students with mental and/or motor impairments are frequently placed in general physical education classes. However, these students often are unable to attain the expected outcomes in these general settings. Based on the experience of the authors, general physical educators often attempt to modify their goals, objectives, teaching…

  11. OT1_ipascucc_1: Understanding the Origin of Transition Disks via Disk Mass Measurements

    NASA Astrophysics Data System (ADS)

    Pascucci, I.

    2010-07-01

    Transition disks are a distinguished group of few Myr-old systems caught in the phase of dispersing their inner dust disk. Three different processes have been proposed to explain this inside-out clearing: grain growth, photoevaporation driven by the central star, and dynamical clearing by a forming giant planet. Which of these processes lead to a transition disk? Distinguishing between them requires the combined knowledge of stellar accretion rates and disk masses. We propose here to use 43.8 hours of PACS spectroscopy to detect the [OI] 63 micron emission line from a sample of 21 well-known transition disks with measured mass accretion rates. We will use this line, in combination with ancillary CO millimeter lines, to measure their gas disk mass. Because gas dominates the mass of protoplanetary disks our approach and choice of lines will enable us to trace the bulk of the disk mass that resides beyond tens of AU from young stars. Our program will quadruple the number of transition disks currently observed with Herschel in this setting and for which disk masses can be measured. We will then place the transition and the ~100 classical/non-transition disks of similar age (from the Herschel KP "Gas in Protoplanetary Systems") in the mass accretion rate-disk mass diagram with two main goals: 1) reveal which gaps have been created by grain growth, photoevaporation, or giant planet formation and 2) from the statistics, determine the main disk dispersal mechanism leading to a transition disk.

  12. The overlay tester (OT) : comparison with other crack test methods and recommendations for surrogate crack tests.

    DOT National Transportation Integrated Search

    2012-10-01

    Presently, one of the principal performance concerns of hot-mix asphalt (HMA) pavements is premature : cracking, particularly of HMA surfacing mixes. Regrettably, however, while many USA transportation agencies have : implemented design-level tests t...

  13. WHY DO THE ACUTE BEHAVIORAL EFFECTS OT TOLUENE IN RATS DEPEND ON THE ROUTE OF EXPOSURE?

    EPA Science Inventory

    Despite evidence suggesting that the acute effects of organic solvents are related to their concentration in the brain, we have observed route-dependent differences in the acute behavioral effects of toluene. Whereas inhaled toluene disrupts the performance of rats on a visual si...

  14. Applying graphics user interface ot group technology classification and coding at the Boeing aerospace company

    NASA Astrophysics Data System (ADS)

    Ness, P. H.; Jacobson, H.

    1984-10-01

    The thrust of 'group technology' is toward the exploitation of similarities in component design and manufacturing process plans to achieve assembly line flow cost efficiencies for small batch production. The systematic method devised for the identification of similarities in component geometry and processing steps is a coding and classification scheme implemented by interactive CAD/CAM systems. This coding and classification scheme has led to significant increases in computer processing power, allowing rapid searches and retrievals on the basis of a 30-digit code together with user-friendly computer graphics.

  15. OT1_dlis_2: Ammonia as a Tracer of the Earliest Stages of Star Formation

    NASA Astrophysics Data System (ADS)

    Lis, D.

    2010-07-01

    Stars form in molecular cloud cores, cold and dense regions enshrouded by dust. The initiation of this process is among the least understood steps of star formation. Highresolution heterodyne spectroscopy provides invaluable information about the physical conditions (density, temperature), kinematics (infall, outflows), and chemistry of these regions. Classical molecular tracers, such CO, CS, and many other abundant gasphase species, have been shown to freeze out onto dust grain mantles in prestellar cores. However, Nbearing species, in particular ammonia, are much less affected by depletion and are observed to stay in the gas phase at densities in excess of 1e6 cm3. The molecular freezeout has important consequences for the chemistry of dense gas. In particular, the depletion of abundant gasphase species with heavy atoms drives up abundances of deuterated H3+ isotopologues, which in turn results in spectacular deuteration levels of molecules that do remain in the gas phase. Consequently, lines of deuterated Nbearing species, in particular the fundamental lines of ammonia isotopologues, having very high critical densities, are optimum tracers of innermost regions of dense cores. We propose to study the morphology, density structure and kinematics of cold and dense cloud cores, by mapping the spatial distribution of ammonia isotopologues in isolated dense prestellar cores using Herschel/HIFI. These observations provide optimum probes of the onset of star formation, as well as the physical processes that control gasgrain interaction, freezeout, mantle ejection and deuteration. The sensitive, highresolution spectra acquired within this program will be analyzed using sophisticated radiative transfer models and compared with outputs of stateoftheart 3D MHD simulations and chemical models developed by the members of our team.

  16. OT2_dlis_3: Ammonia as a Tracer of the Earliest Stages of Star Formation

    NASA Astrophysics Data System (ADS)

    Lis, D.

    2011-09-01

    Stars form in molecular cloud cores, cold and dense regions enshrouded by dust. The initiation of this process is among the least understood steps of star formation. High!resolution heterodyne spectroscopy provides invaluable information about the physical conditions (density, temperature), kinematics (infall, outflows), and chemistry of these regions. Classical molecular tracers, such CO, CS, and many other abundant gas!phase species, have been shown to freeze out onto dust grain mantles in pre!stellar cores. However, N!bearing species, in particular ammonia, are much less affected by depletion and are observed to stay in the gas phase at densities in excess of 1e6 cm!3. The molecular freeze!out has important consequences for the chemistry of dense gas. In particular, the depletion of abundant gas!phase species with heavy atoms drives up abundances of deuterated H3+ isotopologues, which in turn results in spectacular deuteration levels of molecules that do remain in the gas phase. Consequently, lines of deuterated N!bearing species, in particular the fundamental lines of ammonia isotopologues, having very high critical densities, are optimum tracers of innermost regions of dense cores. We propose to study the morphology, density structure and kinematics of cold and dense cloud cores, by mapping the spatial distribution of ammonia isotopologues in isolated dense pre!stellar cores using Herschel/HIFI. These observations provide optimum probes of the onset of star formation, as well as the physical processes that control gas!grain interaction, freeze!out, mantle ejection and deuteration. The sensitive, high!resolution spectra acquired within this program will be analyzed using sophisticated radiative transfer models and compared with outputs of state!of!the!art 3D MHD simulations and chemical models developed by the members of our team.

  17. A parametric study ot protease production in batch and fed-batch cultures of Bacillus firmus.

    PubMed

    Moon, S H; Parulekar, S J

    1991-03-05

    Proteolytic enzymes produced by Bacillus species find a wide variety of applications in brewing, detergent, food, and leather industries. Owing to significant differences normally observed in culture conditions promoting cell growth and those promoting production of metabolites such as enzymes, for increased efficacy of bioreactor operations it is essential to identify these sets of conditions (including medium formulation). This study is focused on formulation of a semidefined medium that substantially enhances synthesis and secretion of an alkaline protease in batch cultures of Bacillus firmus NRS 783, a known superior producer of this enzyme. The series of experiments conducted to identify culture conditions that lead to improved protease production also enables investigation of the regulatory effects of important culture parameters including pH, dissolved oxygen, and concentrations of nitrogen and phosphorous sources and yeast extract in the medium on cell growth, synthesis and secretion of protease, and production of two major nonbiomass products, viz., acetic acid and ethanol. Cell growth and formation of the three nonbiomass products are hampered significantly under nitrogen, phosphorous, or oxygen limitation, with the cells being unable to grow in an oxygen-free environment. Improvement in protease production is achieved with respect to each culture parameter, leading in the process to 80% enhancement in protease activity over that attained using media reported in the literature. Results of a few fed-batch experiments with constant feed rate, conducted to examine possible enhancement in protease production and to further investigate repression of protease synthesis by excess of the principal carbon and nitrogen sources, are also discussed. The detailed investigation of stimulatory and repressory effects of simple and complex nutrients on protease production and metabolism of Bacillus firmus conducted in this study will provide useful guidelines for design of bioreactors for production of protease and bulk chemicals by this bacterium.

  18. Initiatives for Containing the Cost of Higher Education. Stretching the Higher Education Dollar. Special Report 1

    ERIC Educational Resources Information Center

    Massy, William F.

    2013-01-01

    In this article, the author offers a comprehensive reform agenda for policymakers interested in cost containment. Massy lays out a series of initiatives that, working in tandem, can promote the larger goal of compelling colleges to spend money wisely. Among the individual reforms Massy proposes are creating a national database of cost-containment…

  19. Microgravimetry and the Measurement and Application of Gravity Gradients,

    DTIC Science & Technology

    1980-06-01

    Neumann, R., 1972, High precision gravimetry--recent develop- ments: Report to Paris Commission of E.A.E.G., Compagnie Generale de Geophysique , Massy...experimentation on vertical gradient: Compagnie Generale de Geophysique , Massy, France. 12. Fajklewicz, Z. J., 1976, Gravity vertical gradient

  20. High-Amplitude, Rapid Photometric Variation of the New Polar Master OT J132104.0+560957.8

    DTIC Science & Technology

    2015-02-05

    channel the captured material onto cyclotron -emitting accretion regions near the WD’s magnetic poles. Because cyclotron emission is heavily beamed, it...of the cyclotron -emitting region can produce dramatic pho- tometric variability modulated at the WD’s spin period (e.g., Gänsicke et al. 2001). A...observations of J1321 could detect the polarized cyclotron emission from the accretion region, conclusively verifying this classification. Figure 1. The

  1. Site Selective Binding of Zn(ll) ot Metallo-b-Lactamase L1 from Stenotrophomonas Maltophilia

    SciTech Connect

    Costello,A.; Periyannan, G.; Yang, K.

    2006-01-01

    Extended X-ray absorption fine structure studies of the metallo-{beta}-lactamase L1 from Stenotrophomonas maltophilia containing 1 and 2 equiv of Zn(II) and containing 2 equiv of Zn(II) plus hydrolyzed nitrocefin are presented. The data indicate that the first, catalytically dominant metal ion is bound by L1 at the consensus Zn1 site. The data further suggest that binding of the first metal helps preorganize the ligands for binding of the second metal ion. The di-Zn enzyme displays a well-defined metal-metal interaction at 3.42 Angstroms. Reaction with the {beta}-lactam antibiotic nitrocefin results in a product-bound species, in which the ring-opened lactam rotates inmore » the active site to present the S1 sulfur atom of nitrocefin to one of the metal ions for coordination. The product bridges the two metal ions, with a concomitant lengthening of the Zn-Zn interaction to 3.62 Angstroms.« less

  2. Evidence of Cholesterol Accumulated in High Curvature Regions: Implication ot the Curvature Elastic Energy for Lipid Mixtures

    SciTech Connect

    Wang,W.; Yang, L.; Huang, H.

    2007-01-01

    Recent experiments suggested that cholesterol and other lipid components of high negative spontaneous curvature facilitate membrane fusion. This is taken as evidence supporting the stalk-pore model of membrane fusion in which the lipid bilayers go through intermediate structures of high curvature. How do the high-curvature lipid components lower the free energy of the curved structure? Do the high-curvature lipid components modify the average spontaneous curvature of the relevant monolayer, thereby facilitate its bending, or do the lipid components redistribute in the curved structure so as to lower the free energy? This question is fundamental to the curvature elastic energy formore » lipid mixtures. Here we investigate the lipid distribution in a monolayer of a binary lipid mixture before and after bending, or more precisely in the lamellar, hexagonal, and distorted hexagonal phases. The lipid mixture is composed of 2:1 ratio of brominated di18:0PC and cholesterol. Using a newly developed procedure for the multiwavelength anomalous diffraction method, we are able to isolate the bromine distribution and reconstruct the electron density distribution of the lipid mixture in the three phases. We found that the lipid distribution is homogenous and uniform in the lamellar and hexagonal phases. But in the distorted hexagonal phase, the lipid monolayer has nonuniform curvature, and cholesterol almost entirely concentrates in the high curvature region. This finding demonstrates that the association energies between lipid molecules vary with the curvature of membrane. Thus, lipid components in a mixture may redistribute under conditions of nonuniform curvature, such as in the stalk structure. In such cases, the spontaneous curvature depends on the local lipid composition and the free energy minimum is determined by lipid distribution as well as curvature.« less

  3. Multilocus phylogenetic analysis of true morels (Morchella) reveals high levels of endemics in Turkey relative ot other regions of Europe

    USDA-ARS?s Scientific Manuscript database

    The present study was conducted to better understand how the phylogenetic diversity of true morels (Morchella) in Turkey compares with species found in other regions of the world. The current research builds on our recently published survey of 10 Turkish provinces and another of the world in which D...

  4. Topographic form of the Coast Ranges of the Cascadia Margin in relation ot coastal uplift rates and plate subduction

    NASA Technical Reports Server (NTRS)

    Kelsey, Harvey M.; Engebretson, David C.; Mitchell, Clifton E.; Ticknor, Robert L.

    1994-01-01

    The Coast Ranges of the Cascadia margin are overriding the subducted Juan de Fuca/Gorda plate. We investigate the extent to which the latitudinal change in attributes related to the subduction process. These attributes include the varibale age of the subducted slab that underlies the Coast Ranges and average vertical crustal velocities of the western margin of the Coast Rnages for two markedly different time periods, the last 45 years and the last 100 kyr. These vertical crustal velocities are computed from the resurveying of highway bech marks and from the present elevation of shore platforms that have been uplifted in the late Quaternary, respectively. Topogarphy of the Coast Ranges is in part a function of the age and bouyancy of the underlying subducted plate. This is evident in the fact that the two highest topographic elements of the Coast Rnages, the Klamath Mountains and the Olympic Mountains, are underlain by youngest subducted oceanic crust. The subducted Blanco Fracture Zone in southernmost Oregon is responsible for an age discontinuity of subducted crust under the Klamath Mountains. The norhtern terminus of hte topographically higher Klamaths is offset to the north relative to the position of the underlying Blanco Fracture Zone, teh offset being in the direction of migration of the farcture zone, as dictated by relative plate motions. Vertical crustal velocities at the coast, derived from becnh mark surveys, are as much as an order of magnitude greater than vertical crustal velocities derived from uplifted shore platforms. This uplift rate discrepancy indicates that strain is accumulating on the plate margin, to be released during the next interplate earthquake. In a latitudinal sense, average Coast Rnage topography is relatively high where bench mark-derived, short-term vertical crustal velocities are highest. Becuase the shore platform vertical crustal velocities reflect longer-term, premanent uplift, we infer that a small percentage of the interseismic strain that accumulates as rapid short-term uplift is not recovered by subduction earthquakes but rather contributes to rock uplift of the Coast Ranges. The conjecture that permanent rock uplift is related to interseismic uplift is consistent with the observation that those segments of the subduction zone subject to greater interseismic uplift rates are at approximately the same latitudes as those segments of the Coast Ranges that have higher magnitudes of rock uplift over the long term.

  5. OT1_rrubin_1: Herschel's Opportunity to Solve the Nebular Abundance Problem While Creating a Legacy Planetary Nebulae Dataset

    NASA Astrophysics Data System (ADS)

    Rubin, R.

    2010-07-01

    Abundance surveys of a large sample of Galactic planetary nebulae (PNe) have led to the discovery of a group of super-metal-rich nebulae whose spectra show prominent optical recombination lines (ORLs) from C, N, O, and Ne ions. The heavy element abundances derived from ORLs for several PNe are a factor >10 higher than those derived from the traditional method based on collisionally excited lines (CELs). This ratio is called the abundance discrepancy factor (adf). A promising proposition to explain the nebular abundance problem posits that these nebulae contain (at least) two distinct regions - one of "normal" electron temperature, Te (~10000 K) and chemical composition (~solar) and another of very low Te (< 1000) that is H-deficient, thus having high metal abundances relative to H. The latter component emits strong heavy element ORLs and IR fine-structure (FS) CELs but essentially no optical/UV CELs. Efforts to directly detect these inclusions in PNe have been unsuccessful to date. However, there is mounting circumstantial evidence for their existence, such as presented in our recent paper that modeled the high-adf PN NGC 6153 using a 3-D photoionization code. The models that included the low Te, H-deficient knots fit most observations far better than did those models without the clumps. With the launch of Herschel, there is finally the capability to perform a test we've been dreaming of. Measurements have shown that the adf varies with position in a PN and is highest close to the central star. The very low Te inclusions must be cooled via FS IR lines. We propose to use Herschel to map the FS IR lines in 5 bright PNe on the largest adf list, to find if these lines peak where the adf peaks. These spectra will also provide a feast for our other team expertise/interests: a legacy dataset of molecular lines to explore PDRs, how the central star interacts with the AGB ejecta and shapes the PN, how the shocks are produced, what comprises the chemistry of the molecular ejecta, and how do PNe evolve.

  6. "[N]ot subject to our sense” : Margaret Cavendish's fusion of Renaissance science, magic and fairy lore.

    PubMed

    Walters, Lisa

    2010-01-01

    This article explores Margaret Cavendish's depictions of alchemy, witchcraft and fairy lore in her scientific treatise Philosophical Letters and in fictional texts from Natures Pictures and Poems and Fancies. Though Cavendish was a dedicated materialist, she appropriates theories of magic from early modern science and folklore into her materialist epistemology. As Cavendish draws upon a fusion of early modern conceptions of magic, she creates a radical theory of matter which not only challenges patriarchy and binary oppositions, but also explores the plurality and mystery that can exist within an infinitely complex material world.

  7. OT2_wlanger_7: Dynamics of Giant Magnetic Gas Loops and Their Connection to the CMZ in the Galactic Center

    NASA Astrophysics Data System (ADS)

    Langer, W.

    2011-09-01

    Understanding the mass transfer and dynamics among the Galactic Center, the disk, and the halo of the Milky Way is fundamental to the study of the evolution of galaxies and star formation. Recently several giant loops of molecular gas (GML) have been found in the Galactic Center from CO maps, which are likely the result of the magnetic Parker instability. There is new evidence of a possible connection between these loops and the Central Molecular Zone as shown in a sparse [CII] sampling made by the Herschel Key Project GOT C+. Here we propose to map various features of the GMLs and the interface region in [CII] with HIFI. We will also map the foot points of the loop, which are thought to be highly shocked regions, in the ortho 110-101 line of water, which is a known shock tracer. With this data we will characterize different ISM components and their flow among these Galactic Center features.

  8. Crystal structure of product-bound complex of UDP-N-acetyl-D-mannosamine dehydrogenase from Pyrococcus horikoshii OT3

    SciTech Connect

    Pampa, K.J., E-mail: sagarikakj@gmail.com; Lokanath, N.K.; Girish, T.U.

    Highlights: • Determined the structure of UDP-D-ManNAcADH to a resolution of 1.55 Å. • First complex structure of PhUDP-D-ManNAcADH with UDP-D-ManMAcA. • The monomeric structure consists of three distinct domains. • Cys258 acting as catalytic nucleophilic and Lys204 acts as acid/base catalyst. • Oligomeric state plays an important role for the catalytic function. - Abstract: UDP-N-acetyl-D-mannosamine dehydrogenase (UDP-D-ManNAcDH) belongs to UDP-glucose/GDP-mannose dehydrogenase family and catalyzes Uridine-diphospho-N-acetyl-D-mannosamine (UDP-D-ManNAc) to Uridine-diphospho-N-acetyl-D-mannosaminuronic acid (UDP-D-ManNAcA) through twofold oxidation of NAD{sup +}. In order to reveal the structural features of the Pyrococcus horikoshii UDP-D-ManNAcADH, we have determined the crystal structure of the product-bound enzyme bymore » X-ray diffraction to resolution of 1.55 Å. The protomer folds into three distinct domains; nucleotide binding domain (NBD), substrate binding domain (SBD) and oligomerization domain (OD, involved in the dimerization). The clear electron density of the UDP-D-ManNAcA is observed and the residues binding are identified for the first time. Crystal structures reveal a tight dimeric polymer chains with product-bound in all the structures. The catalytic residues Cys258 and Lys204 are conserved. The Cys258 acts as catalytic nucleophile and Lys204 as acid/base catalyst. The product is directly interacts with residues Arg211, Thr249, Arg244, Gly255, Arg289, Lys319 and Arg398. In addition, the structural parameters responsible for thermostability and oligomerization of the three dimensional structure are analyzed.« less

  9. ICT & OTs: a model of information and communications technology acceptance and utilisation by occupational therapists (part 2).

    PubMed

    Schaper, Louise; Pervan, Graham

    2007-01-01

    The research reported in this paper describes the development, empirical validation and analysis of a model of technology acceptance by Australian occupational therapists. The study described involved the collection of quantitative data through a national survey. The theoretical significance of this work is that it uses a thoroughly constructed research model, with one of the largest sample sizes ever tested (n=1605), to extend technology acceptance research into the health sector. Results provide strong support for the model. This work reveals the complexity of the constructs and relationships that influence technology acceptance and highlights the need to include sociotechnical and system issues in studies of technology acceptance in healthcare to improve information system implementation success in this arena. The results of this study have practical and theoretical implications for health informaticians and researchers in the field of health informatics and information systems, tertiary educators, Commonwealth and State Governments and the allied health professions.

  10. 75 FR 63107 - Alternatives to the Use of External Credit Ratings in the Regulations of the OTS

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-14

    ..., savings associations must consider the interest rate, credit, liquidity, price and other risks presented... subject only to moderate credit risk, and for whom expectations of default risk over the term of the... securities activities based on the levels and types of risks in its portfolio. As with the credit quality...

  11. XM587E2/XM724 Electronic Time Fuzes: Developmental Test/Operational Test (DT/OT) II Test Phase

    DTIC Science & Technology

    1979-11-01

    unit - da.- aged setbac* spring Sunit - damAged setback pia 140 .4 C) Twenty-five units were subjected to variable testing "to determine tho G level...SAXIP ACCEPT REJECT NO. Ttf7l KSCRIFTI( AG sin4 go flj-. cEI~ *S-FOOTMOP TEsT o 0 (DROPPED IN FIVE DIFFRNT csIllvrATIaNS -- 1 PER OCRIMP... ascue bya i i*ctet FAILURE AND ACTION REPORT 71134 Jj 7113 .I ,...,-... 7 wow o ft.-I I The unIt will not tht. bte the the scalet inpf t to the.uner h

  12. OT1_ebergin_5: A Systematic Survery of the Water D to H Ratio in Hot Molecular Cores

    NASA Astrophysics Data System (ADS)

    Bergin, E.

    2010-07-01

    The D/H ratio of water and the enrichment of HDO relative to H2O in comets, oceans, and interstellar water vapor, has been posited as one of the primary links between chemistry in the cold (T = 10-20 K) dense interstellar medium (ISM) and chemistry in the Solar Nebula. However, there are only ~10 measurements of HDO/H2O, even in hot (T > 100 K) molecular cores, which have the most favorable chemistry (due to fossil evaporation of D-enriched ices) and excitation. In addition the existing measurements have a wide range of uncertainty, making it impossible to discern the presence of source-to-source variations, which could hint at the origin of deuterium enrichments in the dense ISM. We propose here to change this statistic with a systematic survey of HDO and H2O in a sample of 20 hot molecular cores spanning a two order of magnitude range in mass and luminosity. This will increase the number of known water D/H ratios by ~200%. This program is unique in scope for Herschel and requires the uniformity in calibration and high spectral resolution offered by the HIFI instrument. With the stability of HIFI we will be able to derive D/H ratios with significantly less uncertainty. Our observations will be combined with theoretical chemical models to explore the statistics offered by this sample. By looking at a large number of objects with a range of conditions we aim to unlock the secrets of water deuteration in the interstellar space.

  13. Learning in a Simulation-OT in Heart Surgery and the Challenges of the Scientification of Work

    ERIC Educational Resources Information Center

    Langemeyer, Ines

    2014-01-01

    Enhancing competency and collaboration has become a salient topic of the professional debate on medical safety issues. The advantages of simulation-based training scenarios for team communication, routines and critical work procedures especially in operation theatres have been vigorously discussed. However, the literature on simulation-based…

  14. USSR Report, Chemistry

    DTIC Science & Technology

    1985-11-26

    Individual Absorption Bands of Co(II)-Amino Acid Complexes (D. I. Ismailov, A. A. Gornostal’ DOKLADY AKADEMII NAUK TADZHIKSKOY SSR, No 3, Mar 85...Effectiveness of Methods of Modifying Carbon Fiber Materials (0. A. Novikova, V. P. Sergeyev, et al.; PLASTICHESKIYE MASSY , No 3 , Mar 85) 67...Novikov, et al.; PLASTICHESKIYE MASSY, No 3, Mar 85) 69 Heat-Resistant Anticorrosion Protective Film Based on Radiation-Modified Polyethylene (S. S

  15. OT1_kcoppin_1: A Herschel Survey of [OI]63um in 1

    NASA Astrophysics Data System (ADS)

    Coppin, K.

    2010-07-01

    Luminous obscured galaxies likely dominate the total bolometric emission from star-formation at the early epochs of z~2-3, and are most efficiently identified through their (sub)millimetre emission and are so-called submillimetre galaxies (SMGs). The intense starbursts in SMGs are fuelled by their large observed H2 gas reservoirs, as traced by CO interferometric surveys, although the details of how their immense luminosities (L_fir>5x10^12 Lsun) and star-formation rates (~100-1000 Msun/yr) are powered are not well understood: Are SMGs just scaled up ULIRGs with star-formation occurring in a highly-obscured nuclear region (with perhaps some contribution from an AGN)? Or does the star-formation occur in a more extended, cooler component, such as in "normal" star-forming galaxies. One route to tackling this question is to construct a data set of the brightest fine-structure ISM emission lines ([CII] and [OI]) in a well-defined sample of SMGs, which with ancillary CO data, will allow us to study the physics of the ISM and its interplay with the heating source. Similar benchmark data sets are being compiled by several Herschel programs for local LIRGs and ULIRGs, which will act as a link to help interpret the high-redshift SMG observations. Here we propose a timely and systematic study with the PACS spectrometer of [OI]63um in a flux-limited sample of SMGs with secure spectroscopic redshifts between 0.7

  16. INTERACTION ANALYSES OF BINARY MIXTURES OF CARCINOGENIC PAHS USING MORPHOLOGICAL CELL TRANSFORMATION OF C3H1OT1/2CL8 MOUSE EMBRYO FIBROBLASTS IN CULTURE.

    EPA Science Inventory

    Studies of defined mixtures of carcinogenic polycyclic aromatic hydrocarbons (PAH) have shown three major categories of interactions: antagonism, synergism, and additivity depending on the biological model, tissue, route of exposure, and specific PAH. To understand the bases of t...

  17. Investigation of space shuttle launch vehicle external tank nose configuration effects (model 67-OTS) in the Rockwell International 7 by 7 foot trisonic wind tunnel (IA69)

    NASA Technical Reports Server (NTRS)

    Mennell, R.; Rogge, R.

    1974-01-01

    Wind tunnel aerodynamic investigations were conducted on an 0.015-scale representation of the space shuttle launch configuration. The primary test objectives were to investigate shock wave formation and record the aerodynamic stability and control effects generated by a new external tank nose configuration (MCR 467) at a Mach number of 1.2. Schlieren photographs were taken at angles of attack of -4 deg, 0 deg, and 4 deg, beta = 0 deg with force and pressure data recorded over the alpha range of -4 deg equal to or less than alpha equal to or less than 4 deg at beta = + or - 4 deg. The launch configuration model, consisting of the VL70-00014OA/B Orbiter, the VL78-000041B ET, and the VL77-000036A SRBs, was sting mounted on a 2.5-inch Task type internal balance entering through the ET base region. Wing, body, and base pressure lines for all orifices were routed internally through the model to the sting support system. Parametric variation consisted only of altering the ET nose configuration.

  18. The Influence of Hydrologic Conditions on the Distribution of Baleen Whales in the Antarctic (Raspredelenie Usatykh Kitov v Anarktike v Zabisimosti ot Gidrologicheskikh Uslovii),

    DTIC Science & Technology

    where a semistationary cyclonic system coincides with plankton rich waters upwelling from great depths. Whales locate these feeding grounds through...growth. They also travel parallel to isohalines to remain in the feeding grounds. (Author)

  19. Space shuttle launch vehicle (13 P-OTS) strut support interference effects study in the Rockwell International 7- by 7-foot trisonic wind tunnel (IA68)

    NASA Technical Reports Server (NTRS)

    Rogge, R. L.

    1974-01-01

    Strut support interference investigations were conducted on an 0.004-(-) scale representation of the space shuttle launch vehicle in order to determine transonic and supersonic model support interference effects for use in a future exhaust plume effects study. Strut configurations were also tested. Orbiter, external tank, and solid rocket booster pressures were recorded at Mach numbers 0.9, 1.2, 1.5, and 2.0. Angle of attack and angle of sideslip were varied between plus or minus 4 degrees in 2 degree increments. Parametric variations consisted only of the strut configurations.

  20. Subject and authorship of records related to the Organization for Tropical Studies (OTS) in BINABITROP, a comprehensive database about Costa Rican biology.

    PubMed

    Monge-Nájera, Julián; Nielsen-Muñoz, Vanessa; Azofeifa-Mora, Ana Beatriz

    2013-06-01

    BINABITROP is a bibliographical database of more than 38000 records about the ecosystems and organisms of Costa Rica. In contrast with commercial databases, such as Web of Knowledge and Scopus, which exclude most of the scientific journals published in tropical countries, BINABITROP is a comprehensive record of knowledge on the tropical ecosystems and organisms of Costa Rica. We analyzed its contents in three sites (La Selva, Palo Verde and Las Cruces) and recorded scientific field, taxonomic group and authorship. We found that most records dealt with ecology and systematics, and that most authors published only one article in the study period (1963-2011). Most research was published in four journals: Biotropica, Revista de Biología Tropical/ International Journal of Tropical Biology and Conservation, Zootaxa and Brenesia. This may be the first study of a such a comprehensive database for any case of tropical biology literature.

  1. MASTER OT J015539.85+485955.6 was detected during Fermi alert inspection 3.5h after the trigger time

    NASA Astrophysics Data System (ADS)

    Rebolo, R.; Lipunov, V.; Gorbovskoy, E.; Serra, M.; Lodieu, N.; Israelian, G.; Suarez-Andres, L.; Shumkov, V.; Tyurina, N.; Kornilov, V.; Balanutsa, P.; Kuznetsov, A.; Vlasenko, D.; Gorbunov, I.; Vladimirov, V.; Popova, E.; Buckley, D.; Potter, S.; Kniazev, A.; Kotze, M.; Tlatov, A.; Parhomenko, A. V.; Dormidontov, D.; Senik, V.; Gress, O.; Ivanov, K.; Budnev, N. M.; Yurkov, V.; Sergienko, Yu.; Gabovich, A.; Sinyakov, E.; Krushinski, V.; Zalozhnih, I.; Shurpakov, S.

    2015-11-01

    MASTER-IAC, MASTER-Kislovodsk and MASTER-SAAO was pointed to the FERMI GBM GRB151107B (Stanbro, Meegan, GCN #18570 ) at 2015-11-07 20:25:52(/59s/58s) UT (R.Rebolo et al., GCN #18576 ). There were the prompt pointing observations because duration of the GRB was ~140s . After 5 minutes of the alert observations of the error-box center, MASTER telescopes in IAC and Kislovodsk started the inspect survey inside large Fermi error box (ra=00 42 28 dec=+48 48 58 r=4.533300) obtained by GCN socket.

  2. Wind tunnel test of the 0.019 scale space shuttle integrated vehicle (model 14-OTS) in the CALSPAN 8-foot transonic wind tunnel (IA36), volume 2

    NASA Technical Reports Server (NTRS)

    Hardin, R. B.; Burrows, R. R.

    1975-01-01

    A test is presented which was performed to determine the effect of cold jet gas plumes generated from main propulsion system and solid rocket motor nozzles on: (1) six-component force and moment data, (2) wing static pressures, (3) wing hinge moment, (4) elevon hinge moment, (5) rudder hinge moment, and (6) orbiter MPS nozzle pressure loads. The effects of rudder deflection, nozzle gimbal angle, and plume size were also obtained.

  3. OT1_tvelusam_2: HIFI studies of the small-scale structures in the Galactic diffuse clouds with [CII] and [CI

    NASA Astrophysics Data System (ADS)

    Velusamy, T.

    2010-07-01

    The 1.9 THz [CII] observations provide a powerful probe of warm diffuse clouds, because they can observe them in emission and are useful as a tracer of their molecular H2 not directly traced by CO or other means. HIFI observations of [CII] provide a high resolution of 12 arcsec, better than that for single dish CO (> 30 arcsec) maps, and much better than HI (>30 arcsec). Thus with HIFI we have an opportunity probe the small scale structures in diffuse clouds in the inner Galaxy at distances > 3 kpc. To study the structure of diffuse ISM gas at small scales we propose HIFI maps of 1.9 THz (158 micron) [CII] line emission in a selection of 16 lines of sight (LOSs) towards the inner Galaxy, which are also being observed as part of the GOT C+ survey of [CII] in the Galactic plane. GOT C+ provides mainly single point spectra without any spatial data. Maps of [CII] will constrain better the cloud properties and models when combining [CII] and HI data. The proposed OTF X map will be along the longitude and latitude centered on 18 selected GOT C+ LOS over a length of 3 arcmin in each direction, which is adequate enough to provide sufficient spatial information on the small scale structures at larger distances (>3 kpc) and to characterize the CII filling factor in the larger beams of the ancillary (HI, CO, and CI data). The [CI] 609 & 370micron and the 12CO(7-6) (which lies within the CI band) are excellent diagnostics of the physical conditions of transition clouds and PDRs. We will use the ratio of the [CI] lines to constrain the kinetic temperature and volume density of the CII/CI/CO transition zones in molecular clouds using radiative transfer codes. We also propose OTF X maps in both the [CI] lines for all CII target LOSs. We anticipate fully resolved structural data in [CII] on at least 300 velocity resolved clouds along with their [CI] emissions. We request a total of 33.2 hrs of HIFI observing time.

  4. [Characteristics of floor litter and soil arthropod community in different types ot subtropical forest in Ailao Mountain of Yunnan, Southwest China].

    PubMed

    Yang, Zhao; Yang, Xiao-Dong

    2011-11-01

    By using line transect method, an investigation was conducted on the floor litter and soil arthropod community in a mid mountain wet evergreen broad-leaved forest, a mossy dwarf forest, and a Populus bonatii forest in Ailao Mountain of Yunnan in April (dry and hot season), June (rainy season), and December (dry and cold season), 2005. In both dry and rainy seasons, the existing floor litter mass, C storage, and C/N ratio in the three forests all increased in the order of mossy dwarf forest > P. bonatii forest > evergreen broad-leaved forest, but the N storage had less difference. In the floor litter layer of the forests, Acari and Collembola were the dominant groups of soil arthropod community, while Diptera larvae, Coleoptera, ants, and Homoptera were the common groups. The Sorenson coefficients of soil arthropod community in the three forests were extremely great. No significant differences were observed in the soil arthropod density (ind x m(-2)) in the floor litter layer among the three forests, but the relative density (ind x g(-1)) of soil arthropods was higher in the evergreen broad-leaved forest and P. bonatii forest than in the mossy dwarf forest. In the three forests, the density of soil arthropods was significantly higher in dry season than in rainy season, but the Shannon diversity index had less difference. There were significant positive correlations between the existing floor litter mass and the individual density (ind x m(-2)) and dominant groups of soil arthropod communities in dry and hot season (April), but negative correlations between the existing floor litter mass and the relative density (ind x g(-1)) of soil arthropod communities and Acari in dry and cold season (December). The individual densities of Collembola and Coleoptera also had positive correlations with the N storage of the existing floor litter mass in the three forests. It was considered that the floor litter and the development of soil arthropod community in the litter layer of the subtropical forests in Ailao Mountain had a close relation with the vegetation structure of the forests, and the individual density and the diversity of the soil arthropod community were controlled by the floor litter, whereas the environmental factors such as temperature and moisture in the forests also had obvious effects on the seasonal dynamics of the individual density of the soil arthropods.

  5. Study of structure-function relationships in proteins: Techniques and applications ot cytochrome c: Final report January 15, 1988--January 14, 1989

    SciTech Connect

    Goldstein, D.A.; Rackovsky, S.R.

    1989-08-01

    During the initial period of this work we explored the differential geometry results which had been used to explain the structure-function relationships in the set of yeast iso-1-cytochrome c mutants studied under the initial contract. In addition we continued the development of techniques which would permit the structural characterization and comparison of proteins in a very efficient manner. We have expanded the studies based on the characterization of the structural preferences of various residues in a sample of twenty six globular proteins. It has been demonstrated that the overall structural preferences and the amino acid specific preferences seen in themore » analysis carried out at the five alpha carbon level can not be explained by the results of the analysis carried out at the four alpha carbon level. Thus the structural preferences seen must be described by considering groups of five or more residues. We do no yet have enough data to extend the analysis to the six alpha carbon unit level. We have also verified that the yeast/tuna structural analogy which we used before was justified, and have performed a conformational energy minimization of the reduced yeast cytochrome c crystal data in order to have a baseline for the study of mutant proteins. 6 refs.« less

  6. Hypersonic aeroheating test of space shuttle vehicle: Configuration 3 (model 22 OTS) in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH20), volume 2

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.; Lockman, W. K.

    1975-01-01

    The model tested was an 0.0175-scale version of the vehicle 3 space shuttle configuration. Temperature measurements were made on the launch configuration, orbiter plus tank, orbiter alone, tank alone, and solid rocket booster (SRB) alone to provide heat transfer data. The test was conducted at free stream Mach numbers of 5.3 and 7.3 and at free stream Reynolds numbers of 1.5, 3.7, 5.0, and 7.0 million per foot. The model was tested at angles of attack from -5 deg to 20 deg and side slip angles of -5 deg and 0 deg.

  7. [Comparison of the cleansing effect of two micromotor-driven endodontic hand pieces. I. Comparison ot the cleansing effect of the two handpieces].

    PubMed

    Bartha, K; Rocca, J P

    1993-11-01

    The aim of the study was to assess the cleansing-effect of two endodontic handpieces (Excalibur, W & H; MM 1400 Micro Méga) in root canals that were previously manually prepared, and treated with chlor-(Neomagnol) and citric acid containing solutions. The analyses were performed by scanning electron microscope (SEM). The elimination of the Smear Layer (SL) with Neomagnol was not successful with either of the two handpieces. The cleansing of the Superficial Debris (SD) with Neomagnol activated by MM 1400 was significantly more effective in the apical (p < 0.05) and in the middle (p < 0.01) third of the root-canal, than by Excalibur. In contrast, SL was better eliminated by Excalibur using citric acid, significant differences--when compared with MM 1400--could be seen in the middle third (p < 0.05) of the root-canal. The handpieces seem to have their own specific characteristics: thus MM 1400 was better in eliminating the SD (used with Neomagnol), while Excalibur was superior in eliminating the SL (used with citric acid).

  8. INHIBITION OF INDUCED DIFFERENTIATION OF C3H/1OT 1/2 CLONE 8 MOUSE EMBRYO CELLS BY TUMOR PROMOTERS

    EPA Science Inventory

    C3H/10T 1/2 cells were induced to differentiate into muscle cells by treatment with 5-azacytidine, and the effects of tumor promoters, nonpromoters, and inhibitors of tumor promotion on this induced differentiation were investigated. Cell morphology was dramatically changed withi...

  9. Laboratory and outside the laboratory measurements of ginned and ot ginned cotton for fiber micronaire and maturity by portable Near Infrared (NIR) Instruments

    USDA-ARS?s Scientific Manuscript database

    Micronaire is a key cotton quality assessment property, impacting downstream fiber processing and dye consistency. A component of micronaire is fiber maturity (degree of secondary wall development). Historically, micronaire and maturity are measured in a laboratory under tight environmental condit...

  10. INDUCTION OF GENOTOXIC DAMAGE IS NOT HIGHLY CORRELATED WITH THE ABILITY OT METHYLATE ARSENITE IN VITRO IN THE LEUKOCYTES OF 4 MAMMALIAN SPECIES

    EPA Science Inventory


    Abstract

    Arsenic is a natural drinking water contaminant that impacts the health of large populations of people throughout the world; however, the mode or mechanism by which arsenic induces cancer is unclear. In a series of in vitro studies, we exposed leukocytes from...

  11. Software Supportability Risk Assessment in OT&E (Operational Test and Evaluation): Literature Review, Current Research Review, and Data Base Assemblage.

    DTIC Science & Technology

    1984-09-28

    variables before simula- tion of model - Search for reality checks a, - Express uncertainty as a probability density distribution. a. H2 a, H-22 TWIF... probability that the software con- tains errors. This prior is updated as test failure data are accumulated. Only a p of 1 (software known to contain...discusssed; both parametric and nonparametric versions are presented. It is shown by the author that the bootstrap underlies the jackknife method and

  12. An investigation of drag reduction fairings on the space shuttle vehicle 5 configuration (model 74-OTS) in the MSFC 14 inch trisonic wind tunnel (FA14)

    NASA Technical Reports Server (NTRS)

    Ramsey, P. E.

    1976-01-01

    An experimental investigation was conducted in the MSFC 14-inch TWT (FA14, TWT 600) to determine the static stability and drag on a 0.004 scale model of the shuttle ascent configuration. The primary objective was to study the possibility of reducing the launch vehicle drag by using Orbiter/ET/SRB fairings, streamlined orbiter fore and aft attach structures, SRB and ET alternative nose configurations, and devices for modifying the flow between the orbiter and ET. The secondary objective was to determine the longitudinal and directional characteristics of the ascent configuration with the most promising of the drag reduction devices installed. Data were obtained for a Mach number range of 0.6 through 4.96 and angles of attack from -5 through 5 degrees at zero degrees side slip angle.

  13. Hypersonic aeroheating test of space shuttle vehicle configuration 3 (model 22-OTS) in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH20), volume 1

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.; Lockman, W. K.

    1975-01-01

    The results of hypersonic wind tunnel testing of an 0.0175 scale version of the vehicle 3 space shuttle configuration are presented. Temperature measurements were made on the launch configuration, orbiter plus tank, orbiter alone, tank alone, and solid rocket booster alone to provide heat transfer data. The test was conducted at free-stream Mach numbers of 5.3 and 7.3 and at free-stream Reynolds numbers of 1.5 million, 3.7 million, 5.0 million, and 7.0 million per foot. The model was tested at angles of attack from -5 deg to 20 deg and side slip angles of -5 deg and 0 deg.

  14. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use in...

  15. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use in...

  16. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use in...

  17. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use in...

  18. 33 CFR 150.445 - When is oil in a single point mooring-oil transfer system (SPM-OTS) displaced with water?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the pipeline end manifold must be closed whenever: (1) A storm warning forecasts weather conditions... vessel is about to depart the SPM because of storm conditions; or (3) The SPM is not scheduled for use in...

  19. 75 FR 42833 - Prohibited Service at Savings and Loan Holding Companies

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ....treas.gov . OTS will post comments and the related index on the OTS Internet Site at http://www.ots... regulations applicable to savings associations under Regulation DD. Regulation DD implements the Truth in...

  20. New Fiscal Realities in Higher Education. CPRE Finance Briefs.

    ERIC Educational Resources Information Center

    Consortium for Policy Research in Education, New Brunswick, NJ.

    This report draws from a study being conducted by William Massy and Andrea Wilger for the Consortium for Policy Research in Education (CPRE). The study examines how academic departments work in order to identify organizational incentives that can improve productivity. The brief offers some definitions of productivity in higher education and…

  1. Helping Children with Attentional Challenges in the Montessori Classroom: Introduction

    ERIC Educational Resources Information Center

    Nehring Massie, Catherine

    2017-01-01

    Catherine Nehring Massie provides important contextual information in considering children with attentional challenges. She discusses the prevalence of attentional challenges in today's culture and the contributing factors. She gives a general overview of the spectrum of attentional challenges and some of the indicators in children. Her history of…

  2. Data Needed for Improving Productivity Measurement in Higher Education

    ERIC Educational Resources Information Center

    Massy, William F.; Sullivan, Teresa A.; Mackie, Christopher D.

    2012-01-01

    William Massy is an emeritus professor and former Vice President for Business and Finance at Stanford University, Teresa Sullivan is President of the University of Virginia, and Christopher Mackie is a Study Director with the National Academies' Committee on National Statistics. This article summarizes the authors' recent National Research Council…

  3. Increase in Spin Injection Efficiency of a CoFe/MgO (100) Tunnel Spin Injector with Thermal Annealing

    DTIC Science & Technology

    2005-01-24

    geometry, the optical selection rules provide a direct correlation between the EL polarization and the spin polarization of the electrons just prior...Holland, Amsterdam, 1984d. 18M. Gurioli, A. Vinattieri, M. Colocci, C. Deparis, J. Massies, G. Neu, A. Bosacchi, and S. Franchi , Phys. Rev. B 44, 3115

  4. Higher Education: Handbook of Theory and Research. Volume VI.

    ERIC Educational Resources Information Center

    Smart, John C., Ed.

    Eleven papers on theory and research in higher education have the following titles and authors: "A Paradigm for Research on Higher Education" (William F. Massy); "Minority Student Access to, and Persistence and Performance in College: A Review of the Trends and Research Literature" (Shirley L. Mow and Michael T. Nettles);…

  5. A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing

    PubMed Central

    Eliava, Marina; Melchior, Meggane; Knobloch-Bollmann, H. Sophie; Wahis, Jérôme; Gouveia, Miriam da Silva; Tang, Yan; Ciobanu, Alexandru Cristian; del Rio, Rodrigo Triana; Roth, Lena C.; Althammer, Ferdinand; Chavant, Virginie; Goumon, Yannick; Gruber, Tim; Petit-Demoulière, Nathalie; Busnelli, Marta; Chini, Bice; Tan, Linette L.; Mitre, Mariela; Froemke, Robert C.; Chao, Moses V.; Giese, Günter; Sprengel, Rolf; Kuner, Rohini; Poisbeau, Pierrick; Seeburg, Peter H.; Stoop, Ron; Charlet, Alexandre; Grinevich, Valery

    2017-01-01

    SUMMARY Oxytocin (OT) is a neuropeptide elaborated by the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei. Magnocellular OT neurons of these nuclei innervate numerous forebrain regions and release OT into the blood from the posterior pituitary. The PVN also harbors parvocellular OT cells that project to the brainstem and spinal cord, but their function has not been directly assessed. Here, we identified a subset of approximately 30 parvocellular OT neurons, with collateral projections onto magnocellular OT neurons and neurons of deep layers of the spinal cord. Evoked OT release from these OT neurons suppresses nociception and promotes analgesia in an animal model of inflammatory pain. Our findings identify a new population of OT neurons that modulates nociception in a two tier process: (1) directly by release of OT from axons onto sensory spinal cord neurons and inhibiting their activity and (2) indirectly by stimulating OT release from SON neurons into the periphery. PMID:26948889

  6. A New Population of Parvocellular Oxytocin Neurons Controlling Magnocellular Neuron Activity and Inflammatory Pain Processing.

    PubMed

    Eliava, Marina; Melchior, Meggane; Knobloch-Bollmann, H Sophie; Wahis, Jérôme; da Silva Gouveia, Miriam; Tang, Yan; Ciobanu, Alexandru Cristian; Triana Del Rio, Rodrigo; Roth, Lena C; Althammer, Ferdinand; Chavant, Virginie; Goumon, Yannick; Gruber, Tim; Petit-Demoulière, Nathalie; Busnelli, Marta; Chini, Bice; Tan, Linette L; Mitre, Mariela; Froemke, Robert C; Chao, Moses V; Giese, Günter; Sprengel, Rolf; Kuner, Rohini; Poisbeau, Pierrick; Seeburg, Peter H; Stoop, Ron; Charlet, Alexandre; Grinevich, Valery

    2016-03-16

    Oxytocin (OT) is a neuropeptide elaborated by the hypothalamic paraventricular (PVN) and supraoptic (SON) nuclei. Magnocellular OT neurons of these nuclei innervate numerous forebrain regions and release OT into the blood from the posterior pituitary. The PVN also harbors parvocellular OT cells that project to the brainstem and spinal cord, but their function has not been directly assessed. Here, we identified a subset of approximately 30 parvocellular OT neurons, with collateral projections onto magnocellular OT neurons and neurons of deep layers of the spinal cord. Evoked OT release from these OT neurons suppresses nociception and promotes analgesia in an animal model of inflammatory pain. Our findings identify a new population of OT neurons that modulates nociception in a two tier process: (1) directly by release of OT from axons onto sensory spinal cord neurons and inhibiting their activity and (2) indirectly by stimulating OT release from SON neurons into the periphery. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. Results of heat transfer tests of an 0.0175-scale space shuttle vehicle model 22 OTS in the NASA-Ames 3.5-foot hypersonic wind tunnel (IH3), volume 4

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Lockman, W. K.

    1975-01-01

    Heat-transfer data for the 0.0175-scale Space Shuttle Vehicle 3 are presented. Interference heating effects were investigated by a model build-up technique of Orbiter alone, tank alone, second, and first stage configurations. The test program was conducted in the NASA-Ames 3.5-Foot Hypersonic Wind Tunnel at Mach 5.3 for nominal free-stream Reynolds number per foot values of 1.5 x 1,000,000 and 5.0 x 1,000,000.

  8. Aeroheating (pressure) characteristics on a 0.10-scale version of the vehicle 3 space shuttle configuration (26-OTS) in the Langley Research Center 4-foot wind tunnel (IH4)

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.

    1976-01-01

    Results of wind tunnel tests, conducted at the Langley Research Center Unitary Plan Wind Tunnel, are presented. The model tested was an 0.010-scale version of the Vehicle 3 Space Shuttle Configuration. Pressure measurements were made on the launch configuration, Orbiter alone, external tank alone, and solid rocket booster alone, to provide heat transfer pressure data. The tests were conducted for a Mach number range from 2.36 to 4.6 and Reynolds number range from 1.2 to 5 million per foot. The model was tested at angles of attack from -10 to 20 deg for a sideslip angle range from -5 to +5 deg, and at sideslip angles from -5 to 48 deg for 0 deg angle of attack. Tabulated data are given and photographs of the test configuration are shown.

  9. Aeroheating (pressure) characteristics on a 0.010-scale version of the vehicle 3 space shuttle configuration (26-OTS) in the Langley Research Center 4-foot wind tunnel (IH4), volume 1

    NASA Technical Reports Server (NTRS)

    Kingsland, R. B.

    1976-01-01

    The results of wind tunnel tests conducted on a 0.010-scale version of the Vehicle 3 Space Shuttle Configuration were presented. Pressure measurements were made on the launch configuration, orbiter alone, external tank alone, and solid rocket booster alone, to provide heat transfer pressure data. The tests were conducted for a Mach number range from 2.36 to 4.6 and Reynolds number range from 1.2 to 5 million per foot. The model was tested at angles of attack from -10 deg to 20 deg for a sideslip angle range from -5 deg to +5 deg and at sideslip angles from -5 deg to 48 deg for 0 deg angle of attack.

  10. Results of transonic tests in the NASA/MSFC 14-inch trisonic wind tunnel on a 0.004-scale model (74-OTS) space shuttle launch vehicle (FA25)

    NASA Technical Reports Server (NTRS)

    Lundy, T. E.

    1979-01-01

    The primary objective of the test was to determine the aerodynamic increments due to the attach structure. Secondary objectives were to determine the effects of: (1) orbiter nose mold line changes; (2) wire bundle fairings on data measurements; and (3) flow angularity. The scale model was tested over the Mach range from 0.60 to 1.25 at angles of attack and sideslip from -8 to +8 deg. The total pressure was 22 psia for all runs. Six-component orbiter data were obtained from a balance in the orbiter which was sting supported. The external tank was attached to the solid rocket booster, each of which was sting supported. An alternate two sting/two balance arrangement was also tested with a single sting and balance in the external tank measuring combined ET/SRB aero data replacing the two stings in the SRB's. Two runs were also made at Mach number 4.96 with the two SRB's removed. The aerodynamic coefficients obtained are tabulated as a function of angle of attack or sideslip for each Mach number value.

  11. Transition heating rates obtained on a matted and isolated 0.006 scale model (41-OT) space shuttle orbiter and external tank in the NASA/LaRC variable density hypersonic tunnel (IH17)

    NASA Technical Reports Server (NTRS)

    Cummings, J.

    1976-01-01

    Model information and data obtained from wind tunnel tests performed on a 0.006 scale model of the Rockwell International space shuttle orbiter and external tank in the 18 inch Variable Density Hypersonic Wind Tunnel (VDHT) at NASA Langley Research Center are presented. Tests were performed at a Mach number of 8.0 over a Reynolds Number range from 0.1 to 10.0 million per foot at 0 deg and -5 deg angle of attack and 0 deg sideslip angle. Transition heating rates were determined using thin skin thermocouples located at various locations on the orbiter and ET. The test was conducted in three stages: orbiter plus external tank (mated configuration); orbiter alone, and external tank alone. The effects of boundary layer trips were also included in the test sequence. The plotted results presented show the effect of configuration interference on the orbiter lower surface and on the ET. Tabulated data are given.

  12. Results of transonic wind tunnel tests on an 0.010-scale space shuttle mated vehicle model 72-OTS in the LaRC 8-foot TPT (IA43)

    NASA Technical Reports Server (NTRS)

    Petrozzi, M. T.; Milam, M. D.

    1975-01-01

    Experimental aerodynamic investigations were conducted in NASA/Langley 8-Foot transonic pressure tunnel on a sting mounted 0.010-scale outer mold line model of 104A/B configuration of the Rockwell International space shuttle vehicle. Component aerodynamic force and moment data and base and balance cavity pressures were recorded over an angle of attack range of -10 deg to +10 deg at Mach numbers of 0.6, 0.8, 0.9, 0.98, 1.13, and 1.2. Selected configurations were tested at sideslip angles from -10 deg to +10 deg. For all configurations involving the orbit, wing bending and torsion were measured on the right wing. Inboard elevon setting of 0 deg, +4 deg and +8 deg and outboard settings of 0 deg, +4 deg and +8 deg were tested.

  13. Base pressure and heat transfer tests of the 0.0225-scale space shuttle plume simulation model (19-OTS) in yawed flight conditions in the NASA-Lewis 10x10-foot supersonic wind tunnel (test IH83)

    NASA Technical Reports Server (NTRS)

    Foust, J. W.

    1979-01-01

    Wind tunnel tests were performed to determine pressures, heat transfer rates, and gas recovery temperatures in the base region of a rocket firing model of the space shuttle integrated vehicle during simulated yawed flight conditions. First and second stage flight of the space shuttle were simulated by firing the main engines in conjunction with the SRB rocket motors or only the SSME's into the continuous tunnel airstream. For the correct rocket plume environment, the simulated altitude pressures were halved to maintain the rocket chamber/altitude pressure ratio. Tunnel freestream Mach numbers from 2.2 to 3.5 were simulated over an altitude range of 60 to 130 thousand feet with varying angle of attack, yaw angle, nozzle gimbal angle and SRB chamber pressure. Gas recovery temperature data derived from nine gas temperature probe runs are presented. The model configuration, instrumentation, test procedures, and data reduction are described.

  14. Aerodynamic results of a separation effects test conducted in the AEDC 40 by 40 inch tunnel A facility on the Rockwell International launch configuration 3 (model-OTS) integrated vehicle (IA13), volume 1

    NASA Technical Reports Server (NTRS)

    Campbell, J. H., II

    1975-01-01

    Experimental aerodynamic investigations were conducted from July 5 through July 17, 1973, on a 0.01 scale model. The AEDC captive trajectory system was utilized in conjunction with the tunnel primary sector to obtain grid-type data for external tank abort from the orbiter, and for nominal separation of one solid rocket booster from the orbiter-tank combination. Booster separation was investigated with and without separation motors plume simulation. The plumes were generated by eight M sub j = 2.15 nozzles using a 1500 psia cold air supply. Free stream data were obtained for all models (orbiter, tank, orbiter-tank, and right-hand booster) to provide baselines for evaluation of proximity effects.

  15. Heat transfer tests on a 0.01-scale Rockwell configuration 3 space shuttle orbiter and tank (37-OT) in the Calspan 48-inch hypersonic shock tunnel (OH12/IH21), volume 1

    NASA Technical Reports Server (NTRS)

    Kotch, M.

    1975-01-01

    Model information and data are presented from wind tunnel tests conducted on 0.01-scale models of the space shuttle orbiter and external tank. These tests were conducted in a hypersonic shock tunnel to determine heating rates on ascent and reentry configurations at various Reynolds numbers, Mach numbers, and angles of attack.

  16. Data report for tests on the heat transfer effects of the 0.0175-scale Rockwell International Space Shuttle Vehicle model 22-OT in the AEDC 50-inch B wind tunnel (OH4B), volume 3

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Grifall, W. J.; Martindale, W.

    1975-01-01

    Results of wind tunnel heat transfer tests of 0.0175-scale Rockwell International Space Shuttle Vehicle configurations for orbiter alone, tank alone, and orbiter plus external tank are presented. Body flap shielding of SSME's during simulated entry was also investigated. The tests were conducted at Mach 8 for thirteen Reynolds number per foot values ranging from 0.5 million to 3.72 million.

  17. Data report for tests on the heat transfer effects of the 0.0175 scale Rockwell International Space Shuttle Vehicle model 22-OT in the AEDC 50 inch B wind tunnel (0H4B), volume 1

    NASA Technical Reports Server (NTRS)

    Foster, T. F.; Grifall, W. J.; Martindale, W.

    1975-01-01

    Results of wind tunnel heat transfer tests of 0.0175-scale Rockwell International Space Shuttle Vehicle configurations for orbiter alone, tank alone, and orbiter plus external tank are presented. Body flap shielding of SSME's during simulated entry was investigated. The tests were conducted at Mach 8 for thirteen Reynolds number.

  18. JETCAL 2000R Analyzer H337PA-603 Operational Test and Evaluation (OT&E) on H-53 E/J Aircraft and H46 D/E Aircraft

    DTIC Science & Technology

    2003-01-28

    Proposal Title PORTABLE TEST CELL - JETCAL 2000(R) Lead Proposer HOWELL INSTRUMENTS Military Customer NAVY/MARINE H53...B-4 Proposal Title PORTABLE TEST CELL - JETCAL 2000(R) Lead Proposer HOWELL INSTRUMENTS Military Customer...TEST CELL - JETCAL 2000(R) Lead Proposer HOWELL INSTRUMENTS Military Customer NAVY/MARINE H53 AIRCRAFT Baseline Costs -- DoD’s Costs When COSSI is NOT

  19. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot Transonic wind tunnel (IA613A), volume 1

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Lemoine, P.

    1992-01-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e. top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  20. Results of an external tank separation test in AEDC/VKF tunnel B on 0.010-scale replica of space shuttle vehicle model 52-OT(IA17A), Volume 1

    NASA Technical Reports Server (NTRS)

    Spangler, R. H.; Daileda, J. J.

    1975-01-01

    Tests were conducted on scale models of the space shuttle orbiter and external tank (ET) to determine the aerodynamic interactions during a return to launch site abort separation. The orbiter model was built to vehicle 3 configuration lines (139B) and the ET model approximated the vehicle 5 configurations with protuberances and attach hardware. For these investigations the orbiter was mounted on the primary support system and the external tank was mounted on the captive trajectory system. Six-component data were obtained for each vehicle at various orbiter angles of attack and sideslip for a range of relative angular and linear displacements of the ET from the orbiter.

  1. Examining Rotational Variability in the Upper Tropospheres and Lower Stratospheres of Uranus and Neptune from Herschel PACS OT1 Observations: Implications for the Stability of Temperature and Compositional Structure

    NASA Astrophysics Data System (ADS)

    Orton, G.; Feuchtgruber, H.; Fletcher, L.; Lellouch, E.; Moreno, R.; Billebaud, F.; Cavalie, T.; Decin, L.; Dobreijecvic, M.; Encrenaz, T.; Hartogh, P.; Jarchow, C.; Lara, L. M.; Liu, J.

    2012-04-01

    The power of high-resolution submillimeter spectroscopy of Uranus and Neptune was put to use to survey the rotational variability of stratospheric and tropospheric constituents of their atmospheres. These observations were motivated by the surprising discovery of as much as 12% rotational variability of emission from stratospheric constituents in the atmosphere of Uranus by the Spitzer Infrared Spectrometer and the detection of spatial variability in thermal images of Neptune's stratospheric emission (Orton et al. 2007, Astron. & Astrophys 473, L3). Our observing program consisted of three separate sequences of observations to look at the strongest lines of H2O in the high-resolution PACS spectra of both planets, whose upwelling radiance emerges from the same vertical region as the Spitzer IRS observations of Uranus and ground-based images of Neptune, and the strongest line of CH4 in the PACS spectrum of Neptune. We omitted measurements of CH4 lines in Uranus, which are almost non-detectable. We added the strongest HD line in Uranus to measure variability of tropospheric temperatures that could modulate stratospheric CH4 abundances through local cold-trapping and the strongest two HD lines in Neptune (Lellouch et al. 2010, Astron. & Astrophys. 518, L152) that determine both the tropopause temperature to limit local cold-trapping efficacy and the lower stratospheric temperature, to help differentiate between longitudinal variability of stratospheric H2O and CH4 abundances vs. temperatures. These were repeated over the 17-hour interval that is common to the equatorial rotation periods of both Uranus and Neptune. Although these lines had already been observed in Uranus and Neptune by PACS, no repeat measurements had ever been made to determine longitudinal variability. The observations were consistent with previous measurements, but no significant rotational variability was detected. It is possible that the absence of rotational variability in the HD and CH4 lines is because variability is confined to very low pressures, e.g. much lower than a microbar. However, the absence of variable emission from high-altitude exogenic H2O vapor is harder to explain, unless the variability seen in Uranus by Spitzer and in Neptune from the VLT, is not the result of variations in temperature by in the hydrocarbon abundances. Alternatively, the stratospheres of both planets are variable in time. The cause of such variability is unknown, but spatially confined outbursts have been detected in the visible and near infrared, and they may have as much influence on the stratosphere of Uranus as the great springtime storm in Saturn's northern hemisphere, creating a strong, localized "beacon" of thermal radiation (cf. Fletcher et al. 2011, Science, 332,1413) that could dominate the emission observed over the hemisphere.

  2. Results of Experimental Investigations to Determine External Tank Protuberance Loads Using a 0.03-Scale Model of the Space Shuttle Launch Configuration (Model 47-OTS) in the NASA/ARC Unitary Plan Wind Tunnel, Volume 2

    NASA Technical Reports Server (NTRS)

    Houlihan, S. R.

    1992-01-01

    Data were obtained on a 3-percent model of the Space Shuttle launch vehicle in the NASA/Ames Research Center 11x11-foot and 9x7-foot Unitary Plan Wind Tunnels. This test series has been identified as IA19OA/B and was conducted from 7 Feb. 1980 to 19 Feb. 1980 (IA19OA) and from 17 March 1980 to 19 March 1980 and from 8 May 1980 to 30 May 1980 (IA19OB). The primary test objective was to obtain structural loads on the following external tank protuberances: (1) LO2 feedline; (2) GO2 pressure line; (3) LO2 antigeyser line; (4) GH2 pressure line; (5) LH2 tank cable tray; (6) LO2 tank cable tray; (7) Bipod; (8) ET/SRB cable tray; and (9) Crossbeam/Orbiter cable tray. To fulfill these objectives the following steps were taken: Eight 3-component balances were used to measure forces on various sections of 1 thru 6 above; 315 pressure orifices were distributed over all 9 above items. The LO2 feedline was instrumented with 96 pressure taps and was rotated to four positions to yield 384 pressure measurements. The LO2 antigeyser line was instrumented with 64 pressure taps and was rotated to two positions to yield 128 pressure measurements; Three Chrysler miniature flow direction probes were mounted on a traversing mechanism on the tank upper surface centerline to obtain flow field data between the forward and aft attach structures; and Schlieren photographs and ultraviolet flow photographs were taken at all test conditions. Data from each of the four test phases are presented.

  3. Results of experimental investigations to determine external tank protuberance loads using a 0.03-scale model of the Space Shuttle launch configuration (model 47-OTS) in the NASA/ARC unitary plan wind tunnel, volume 1

    NASA Technical Reports Server (NTRS)

    Houlihan, S. R.

    1992-01-01

    Data were obtained on a 3-percent model of the Space Shuttle launch vehicle in the NASA/Ames Research Center 11x11-foot and 9x7-foot Unitary Plan Wind Tunnels. This test series has been identified as IA190A/B and was conducted from 7 Feb. 1980 to 19 Feb. 1980 (IA190A) and from 17 March 1980 to 19 March 1980 and from 8 May 1980 to 30 May 1980 (IA190B). The primary test objective was to obtain structural loads on the following external tank protuberances: (1) LO2 feedline, (2) GO2 pressure line, (3) LO2 antigeyser line, (4) GH2 pressure line, (5) LH2 tank cable tray, (6) LO2 tank cable tray, (7) Bipod, (8) ET/SRB cable tray, and (9) Crossbeam/Orbiter cable tray. To fulfill these objectives the following steps were taken: (1) Eight 3-component balances were used to measure forces on various sections of 1 thru 6 above. (2) 315 pressure orifices were distributed over all 9 above items. The LO2 feedline was instrumented with 96 pressure taps and was rotated to four positions to yield 384 pressure measurements. The LO2 antigeyser line was instrumented with 64 pressure taps and was rotated to two positions to yield 128 pressure measurements. (3) Three Chrysler miniature flow direction probes were mounted on a traversing mechanism on the tank upper surface centerline to obtain flow field data between the forward and aft attach structures. (4) Schlieren photographs and ultraviolet flow photographs were taken at all test conditions. Data from each of the four test phases are presented.

  4. Flutter tests (IS4) of the 0.0125-scale shuttle reflection plane model 30-OTS in the Langley Research Center 26-inch transonic blowdown tunnel test no. 547

    NASA Technical Reports Server (NTRS)

    Kotch, M. A.

    1974-01-01

    A series of slab wing flutter models with rigid orbiter fuselage, external tank, and SRB models of the space shuttle were tested, in a reflection plane arrangement, in the NASA Langley Research Center's 26-inch Transonic Blowdown Tunnel. Model flutter boundaries were obtained for both a wing-alone configuration and a wing-with-orbiter, tank and SRB configuration. Additional test points were taken of the wing-with-orbiter configuration, as a correlation with the wing-alone condition. A description of the wind tunnel models and test procedures utilized in the experiment are provided.

  5. Wind tunnel tests of an 0.019-scale space shuttle integrated vehicle -2A configuration (model 14-OTS) in the NASA Ames 8 X 7 foot unitary wind tunnel, volume 2. [cold jet gas plumes and pressure distribution

    NASA Technical Reports Server (NTRS)

    Hardin, R. B.; Burrows, R. R.

    1975-01-01

    The purpose of the test was to determine the effects of cold jet gas plumes on (1) the integrated vehicle longitudinal and lateral-directional force data, (2) exposed wing hinge moment, (3) wing pressure distributions, (4) orbiter MPS external pressure distributions, and (5) model base pressures. An investigation was undertaken to determine the similarity between solid and gaseous plumes; fluorescent oil flow visualization studies were also conducted. Plotted wing pressure data is tabulated.

  6. Results of transonic wind tunnel tests on an 0.015-scale space shuttle mated vehicle model (67-ots) in the LaRC 8 foot TPT (IA41)

    NASA Technical Reports Server (NTRS)

    Hardin, R.; Burrows, R. R.

    1974-01-01

    Wind tunnel tests were conducted to obtain aerodynamic force data for Mach numbers from 0.60 to 1.20. Data were obtained for an alpha range of -10 deg to +10 deg (beta = 0 deg beta = 5 deg) and beta range of -10 deg to +10 deg (alpha = 0 deg). Longitudinal and lateral-directional stability and control data were obtained for tank alone, tank plus SRB's, tank plus Orbiter, and mated configuration of tank + Orbiter + SRB's. Also, single-component rudder hinge moment data were obtained at rudder deflections of 0 and -20 deg for each Mach number tested. Plots of aerodynamic coefficients vs. Mach number are presented, using data from both test IA41 and tests LRC-UPWT-1056, 1073 (IA42A/B) for Mach numbers of 1.60 to 4.63. The model tested in IA42A/B was the same model as tested in IA41.

  7. Heat transfer tests of an 0.006-scale thin-skin space shuttle thermocouple model (141-OT) in the Langley Research Center Freon tunnel at M-6 (IH18). [wind tunnel tests

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.

    1976-01-01

    Ascent heating data were obtained at conditions simulating real gas effects at hypersonic Mach numbers. The configurations tested were Orbiter alone, external tank alone, and mated Orbiter and external tank. A boundary layer trip investigation was conducted for all configurations. The test was conducted at Mach 6 and Reynolds number of one half million per foot for 0 deg and -5 deg angle-of-attack. Selected thermocouples were chosen from the Orbiter and external tank to be used for obtaining heat transfer measurements. A maximum of 42 thermocouples could be measured by the facility data acquisition at one time and no attempt was made to record the excess thermocouples located on the model. Photographs of the test configurations are shown.

  8. Heat transfer tests of an 0.006-scale thin-skin space shuttle model (41-OTS) in the Ames 3.5-foot HWT at M equals 5.3 (IH15)

    NASA Technical Reports Server (NTRS)

    Walstad, D. G.; Lockman, W. K.

    1974-01-01

    Data obtained from heat transfer tests of an 0.006-scale space shuttle vehicle in a 3.5-foot hypersonic wind tunnel are presented. The purpose of these tests was to parametrically investigate the ascent heating of the integrated vehicle. Configurations tested were complete for integrated vehicle, orbiter alone, external tank alone, and SRB alone. All configurations were tested with and without transition grit. Testing was conducted at a Mach number of 5.3, and at Reynolds numbers of 2 and 5 million per foot. The angle of attack range varied from 0 to minus 5 degress, execpt for SRB alone, which was tested from minus 5 to 90 degrees. Heat transfer data were obtained from 223 iron-constantan thermocouples attached to thin-skin stainless steel inserts.

  9. An investigation in MSFC 14-inch TWT to determine the static stability characteristics of 0.004-scale model (74-OTS) space shuttle vehicle 5 configuration (IA33), volume 1

    NASA Technical Reports Server (NTRS)

    Allen, E. C.

    1975-01-01

    Wind tunnel tests were conducted to: (1) determine the static stability characteristics of the Shuttle Vehicle 5 configuration; (2) determine the effect on the Vehicle 5 aerodynamic characteristics of External Tank (ET) and Solid Rocket Booster (SRB) nose shape, SRB nozzle shroud flare angle, orbiter to tank fairing, and sting location; (3) provide flow visualization using thin film oil paint; and (4) determine rudder, body flap, and inboard and outboard elevon hinge moments. The mated vehicle model was mounted in three different ways: (1) the orbiter mounted on the balance with the SRB's attached to the tank and the tank in turn attached to the orbiter; (2) the tank mounted on the balance (with the sting protruding through the tank base) with the SRB's and orbiter attached to the tank, and (3) with the tank mounted on the balance and the balance in turn supported by a forked sting entering the nozzle of each SRB, extending forward into the SRB's then crossing over to the tank to provide a balance socket. Data were obtained for Mach numbers from 0.6 through 4.96 at angles-of-attack and -sideslip from -10 to 10 degrees.

  10. Results of investigations conducted in the LaRC 8-foot transonic pressure tunnel using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA93)

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Test procedures, history, and data from the wind tunnel test are presented. Aero-loads were investigated on the updated configuration-5 space shuttle launch vehicle at Mach numbers from 0.600 to 1.205. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed. Various elevon deflection angles were tested with two different forward orbiter-to-external-tank attach-strut configurations. The entire model was supported by means of a balance mounted in the orbiter through its base and suspended from a sting.

  11. Results of investigations conducted in the LaRC 8-foot transonic pressure tunnel using the 0.010-scale 72-OTS model of the space shuttle integrated vehicle (IA93), volume 2

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1976-01-01

    Test procedures, history, and plotted coefficient data are presented for an aero-loads investigation on the updated configuration-5 space shuttle launch vehicle at Mach numbers from 0.600 to 1.205. Six-component vehicle forces and moments, base and sting-cavity pressures, elevon hinge moments, wing-root bending and torsion moments, and normal shear force data were obtained. Full simulation of updated vehicle protuberances and attach hardware was employed.

  12. Results of heat transfer tests of a 0.0175-scale space shuttle vehicle 5 model (60-OTS) in the NASA-Ames Research Center 3.5-foot hypersonic wind tunnel (test IH48)

    NASA Technical Reports Server (NTRS)

    Dye, W. H.; Lockman, W. K.

    1976-01-01

    Heat transfer data are presented for a .0175-scale model of the Rockwell International Space Shuttle Vehicle 5. The primary purpose of these tests was to obtain aerodynamic interference heating data on the external tank in the tank alone, second-, and first-stage configurations. Data were also obtained on the Orbiter and solid rocket boosters. Nominal Mach Nos. of 5.2 and 5.3 at nominal freestream unit Reynolds numbers of 1.5 and 5.0 million per foot, respectively, were investigated. Photographs of the tested configurations and test equipment are shown.

  13. Results of wind tunnel tests of an ASRM configured 0.03 scale Space Shuttle integrated vehicle model (47-OTS) in the AEDC 16-foot transonic wind tunnel, volume 2

    NASA Technical Reports Server (NTRS)

    Marroquin, J.; Lemoine, P.

    1992-01-01

    An experimental Aerodynamic and Aero-Acoustic loads data base was obtained at transonic Mach numbers for the Space Shuttle Launch Vehicle configured with the ASRM Solid Rocket Boosters as an increment to the current flight configuration (RSRB). These data were obtained during transonic wind tunnel tests (IA 613A) conducted in the Arnold Engineering Development Center 16-Foot transonic propulsion wind tunnel from March 27, 1991 through April 12, 1991. This test is the first of a series of two tests covering the Mach range from 0.6 to 3.5. Steady state surface static and fluctuating pressure distributions over the Orbiter, External Tank and Solid Rocket Boosters of the Shuttle Integrated Vehicle were measured. Total Orbiter forces, Wing forces and Elevon hinge moments were directly measured as well from force balances. Two configurations of Solid Rocket Boosters were tested, the Redesigned Solid Rocket Booster (RSRB) and the Advanced Solid Rocket Motor (ASRM). The effects of the position (i.e., top, bottom, top and bottom) of the Integrated Electronics Assembly (IEA) box, mounted on the SRB attach ring, were obtained on the ASRM configured model. These data were obtained with and without Solid Plume Simulators which, when used, matched as close as possible the flight derived pressures on the Orbiter and External Tank base. Data were obtained at Mach numbers ranging from 0.6 to 1.55 at a Unit Reynolds Number of 2.5 million per foot through model angles of attack from -8 to +4 degrees at sideslip angles of 0, +4 and -4 degrees.

  14. Results of investigations of an 0.010-scale 140A/B configuration (model 72-OTS) of the Rockwell International space shuttle orbiter in the NASA/Langley Research Center unitary plan wind tunnel

    NASA Technical Reports Server (NTRS)

    Petrozzi, M. T.; Milam, M. D.

    1975-01-01

    Experimental aerodynamic investigations were conducted in the NASA/Langley unitary plan wind tunnel on a sting mounted 0.010-scale outer mold line model of the 140A/B configuration of the Rockwell International Space Shuttle Vehicle. The primary test objectives were to obtain: (1) six component force and moment data for the mated vehicle at subsonic and transonic conditions, (2) effects of configuration build-up, (3) effects of protuberances, ET/orbiter fairings and attach structures, and (4) elevon deflection effects on wing bending moment. Six component aerodynamic force and moment data and base and balance cavity pressures were recorded over Mach numbers of 1.6, 2.0, 2.5, 2.86, 3.9, and 4.63 at a nominal Reynolds number of 20 to the 6th power per foot. Selected configurations were tested at angles of attack and sideslip from -10 deg to +10 deg. For all configurations involving the orbiter, wing bending, and torsion coefficients were measured on the right wing.

  15. Results of oil flow visualization tests of an 0.010-scale model (52-OT) of the space shuttle orbiter-tank mated and orbiter configurations in the AEDC VKF tunnel B (IA17B)

    NASA Technical Reports Server (NTRS)

    Daileda, J. J.

    1975-01-01

    An 0.010-scale model of the space shuttle (orbiter-tank mated and orbiter configurations) was tested in the AEDC VKF Tunnel B to investigate aerodynamic flow patterns. The tests utilized oil flow techniques to visualize the flow patterns. Tunnel free stream Mach number was 7.95 and nominal unit Reynolds number was 3.7 million per foot. Model angle of attack was varied from -5 deg through 10 deg and angle of sideslip was 0 deg and 2 deg. Photographs of resulting oil flow patterns are presented.

  16. Results of an investigation of Reynolds effects on integrated vehicle elevon hinge moments and wing panel loads obtained with 0.010 scale model 72 OTS in the Rockwell trisonic wind tunnel (IA141)

    NASA Technical Reports Server (NTRS)

    Mennell, R. C.

    1976-01-01

    Wind tunnel investigations were conducted on an 0.010-scale representation of the VL70-000140C Integrated Space Shuttle Launch Vehicle. The primary test objective was to obtain Reynolds number effects on orbiter elevon hinge moments and wing bending/torsional moments. Launch vehicle aerodynamic force data were also recorded. The elevon hinge moments, wing bending/torsional moments, and vehicle force data were recorded over an angle of attack range of -6 deg to +6 deg, an angle of sideslip range of -6 deg to +6 deg, at Mach numbers of 0.6, 0.975, 1.05 and 1.25. The Reynolds number was varied from a minimum of 4.5 million/foot to a maximum of 11.5 million/foot. The complete integrated configuration was tested with the orbiter elevons set at 0 deg and deflected to 9 deg on the outboard elevon and 10 deg on the inboard elevon. Testing was conducted in the TWT 19.7% porous transonic test section with the model sting mounted through the orbiter base. All aerodynamic force data were obtained from internal strain gage balance located in the orbiter.

  17. Oxytocin and Social Adaptation: Insights from Neuroimaging Studies of Healthy and Clinical Populations.

    PubMed

    Ma, Yina; Shamay-Tsoory, Simone; Han, Shihui; Zink, Caroline F

    2016-02-01

    Adaptation to the social environment is critical for human survival. The neuropeptide oxytocin (OT), implicated in social cognition and emotions pivotal to sociality and well-being, is a promising pharmacological target for social and emotional dysfunction. We suggest here that the multifaceted role of OT in socio-affective processes improves the capability for social adaptation. We review OT effects on socio-affective processes, with a focus on OT-neuroimaging studies, to elucidate neuropsychological mechanisms through which OT promotes social adaptation. We also review OT-neuroimaging studies of individuals with social deficits and suggest that OT ameliorates impaired social adaptation by normalizing hyper- or hypo-brain activity. The social adaption model (SAM) provides an integrative understanding of discrepant OT effects and the modulations of OT action by personal milieu and context. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Randomized Controlled Trial: Multimodal Anxiety and Social Skill Intervention for Adolescents with Autism Spectrum Disorder

    PubMed Central

    White, Susan W.; Ollendick, Thomas; Albano, Anne Marie; Oswald, Donald; Johnson, Cynthia; Southam-Gerow, Michael A.; Kim, Inyoung; Scahill, Lawrence

    2012-01-01

    Anxiety is common among adolescents with autism spectrum disorders (ASD) and may amplify the core social disability, thus necessitating combined treatment approaches. This pilot, randomized controlled trial (RCT) evaluated the feasibility and preliminary outcomes of the Multimodal Anxiety and Social Skills Intervention (MASSI) program in a sample of 30 adolescents with ASD and anxiety symptoms of moderate or greater severity. The treatment was acceptable to families, subject adherence was high, and therapist fidelity was high. A 16% improvement in ASD social impairment (within-group effect size = 1.18) was observed on a parent-reported scale. Although anxiety symptoms declined by 26%, the change was not statistically significant. These findings suggest MASSI is a feasible treatment program and further evaluation is warranted. PMID:22735897

  19. Development of a cognitive-behavioral intervention program to treat anxiety and social deficits in teens with high-functioning autism.

    PubMed

    White, Susan W; Albano, Anne Marie; Johnson, Cynthia R; Kasari, Connie; Ollendick, Thomas; Klin, Ami; Oswald, Donald; Scahill, Lawrence

    2010-03-01

    Anxiety is a common co-occurring problem among young people with autism spectrum disorders (ASD). Characterized by deficits in social interaction, communication problems, and stereotyped behavior and restricted interests, this group of disorders is more prevalent than previously realized. When present, anxiety may compound the social deficits of young people with ASD. Given the additional disability and common co-occurrence of anxiety in ASD, we developed a manual-based cognitive-behavioral treatment program to target anxiety symptoms as well as social skill deficits in adolescents with ASD [Multimodal Anxiety and Social Skills Intervention: MASSI]. In this paper, we describe the foundation, content, and development of MASSI. We also summarize data on treatment feasibility based on a pilot study that implemented the intervention.

  20. Urinary and plasma oxytocin changes in response to MDMA or intranasal oxytocin administration.

    PubMed

    Francis, Sunday M; Kirkpatrick, Matthew G; de Wit, Harriet; Jacob, Suma

    2016-12-01

    The neuropeptide oxytocin (OT) has received increased experimental attention for its putative role in both normal social functioning and several psychiatric disorders that are partially characterized by social dysfunction (e.g., autism spectrum disorders: ASD). Many human experimental studies measure circulating plasma levels of OT in order to examine the relationship between the hormone and behavior. Urinary OT (uOT) assays offer a simple, easy, and non-invasive method to measure peripheral hormone levels, but the correspondence between uOT and plasma OT (pOT) levels is unclear. Here, we conducted two within-subjects, double-blind studies exploring changes in uOT and pOT levels following administration of two drugs: MDMA, an oxytocin-releasing drug (Study 1), and intranasal oxytocin (INOT: Study 1 and 2). In Study 1, 14 adult participants (2 females) were each administered either oral 1.5mg/kg MDMA or 40IU INOT across two different study sessions. In Study 2, 10 male participants (adolescents and young adults) diagnosed with ASD received either 40IU INOT or placebo across two different sessions. In both studies, blood and urine samples were collected before and after drug administration at each study session. For Study 1, 10 participants provided valid plasma and urine samples for the MDMA session, and 8 provided valid samples for the INOT session. For Study 2, all 10 participants provided valid samples for both INOT and placebo sessions. Pre- and post-administration levels of pOT and uOT were compared. Additionally, correlations between percent change from baseline uOT and pOT levels were examined. Study 1: Plasma OT and uOT levels significantly increased after administration of MDMA and INOT. Furthermore, uOT levels were positively correlated with pOT levels following administration of MDMA (r=0.57, p=0.042) but not INOT (r=0.51, p=0.097). Study 2: There was a significant increase in uOT levels after administration of INOT, but not after administration of

  1. Final Environmental Impact Statement on Debris Removal from Boston Harbor, Massachusetts. Revision.

    DTIC Science & Technology

    1980-05-01

    34Trace Metal Analysis of Boston Harbor Waters and Sediments", July 1972. Storey , D. A., "The Massachusetts Marina Boatyard Industry 1972-1973", Mass...is possible that a feasible re-use alternative will be identified during the final design stage of the project. If this happens, and the method of re...points. Coliform counts in the Outer Harbor routinely exceed the SB standard designated for that area. 2.27 In summary, the Harbor receives a heavy

  2. Military Hybrid Vehicle Optimization and Control

    DTIC Science & Technology

    2012-08-14

    Michigan Technological University August 14, 2012 *UnclassifieJ. Distribution Statement A. Approved for public release. :# 2 3 2 7 1 Abstract...t • • f ... - _.____ ~ - ...__ Comt >osae Ch-8 Urt>an Urt>anllfghwoy Drive Cycle Figure 6: Cycle vs. fuel economy improvement for the Hl\\tll...37 3 , June- August 20011. [6] .J . Cross E. Shaffer, D. Massie. Power and energy architecture for army advanced energy initiative. [7] C. Marnay J

  3. The Applicability of Patented Technologies to Sonobuoys

    DTIC Science & Technology

    1988-06-30

    Ave, Boulder, CO 80301 2. Compagnie Generale de Geophysique 6 Rue Galvani Paris, France 3. EIC Laboratories, Incorporated Newton, MA 02146 4. Edo...Co. 2 Compagnie de Geophysique , France 3 Cooper, Charles E. 2 M Crown Controls Corporation 1 ,6 Cyr, Reginald J. 1 DeltaLab Research, Incorporated 1...Int’l App ....... 7,5 [73) Assignees: Institut Francais du Petrole. OTHER PUBLICATIONS -" Rueil-Malmaison; Compagnie Generale de Geophysique , Massy

  4. A comparison of VLSI architecture of finite field multipliers using dual, normal or standard basis

    NASA Technical Reports Server (NTRS)

    Hsu, I. S.; Truong, T. K.; Shao, H. M.; Deutsch, L. J.; Reed, I. S.

    1987-01-01

    Three different finite field multipliers are presented: (1) a dual basis multiplier due to Berlekamp; (2) a Massy-Omura normal basis multiplier; and (3) the Scott-Tavares-Peppard standard basis multiplier. These algorithms are chosen because each has its own distinct features which apply most suitably in different areas. Finally, they are implemented on silicon chips with nitride metal oxide semiconductor technology so that the multiplier most desirable for very large scale integration implementations can readily be ascertained.

  5. Investigation of the Atmospheric Propagation of Alkali Lasers in a Maritime Environment Using Tunable Diode Laser Atmospheric Spectroscopy

    DTIC Science & Technology

    2013-03-01

    which is given as (6) where is the FWHM of a Doppler broadened lineshape, given as...magnitude higher than the Doppler broadened width, the pressure broadened FWHM is not sufficiently large to entirely dominate the overall FWHM. Because...Electronics. Prentice Hall, USA, 1995. 8. L. S. Rothman, C. P. Rinsland, A. Goldman , S. T. Massie, D. P. Edwards, J.-M. Flaud, A. Perrin, C. Camy

  6. Soldier’s and Sailors’ Civil Relief Act.

    DTIC Science & Technology

    1998-06-01

    insurance company .102 (2) Who has the burden of proof to show "material effect ." In discussing the question of burden of proof, the Supreme...Charlottesville, VA 22903-1781 5. FUNDING NUMBERS NA 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) The Judge Advocate General’ School...U.S. Army 600 Massie Road Charlottesville, VA 22903- 1781 8. PERFORMING ORGANIZATION REPORT NUMBER JA 260 (April 1998) 9. SPONSORING

  7. AN OFFSET FOR AFSOF: COMBINING ADDITIVE MANUFACTURING AND AUTONOMOUS SYSTEMS WITH SWARM EMPLOYMENT

    DTIC Science & Technology

    2016-10-01

    teams composed of autonomous robot players compete in games of soccer .58 Strongly coordinated centralized systems are similar to the distributed...goal in a dynamically changing environment. This is a very active area of research and exemplified by the robot soccer league, a competition where...University, 2013, 23. 63 Massie, Andrew. “Autonomy and the Future Force” Strategic Studies Quarterly, Summer 2016, 146. 64 Zacharias, Greg. "Autonomus

  8. Interacting Coronae of Two T Tauri Stars: First Observational Evidence for Solar-Like Helmet Streamers

    DTIC Science & Technology

    2008-01-01

    for the first time in stars other than the Sun. The complete extent of each helmet streamer above the stellar surface is about 24 R∗ which implies...is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and...Heliospheric Observatory (SOHO) satellite (Schwenn 2006; Suess & Nerney 2004; Vourlidas 2006). Following the analogy with the Sun, Massi & collaborators

  9. COMMUNITY CAPACITY BUILDING FOR REVITALIZATION AND SUSTAINABLE REDEVELOPMENT

    SciTech Connect

    Downing, Melinda; Rosenthall, John; Hudson, Michelle

    2003-02-27

    Capacity building programs help poor and disadvantaged communities to improve their ability to participate in the environmental decision-making processes. They encourage citizen involvement, and provide the tools that enable them to do so. Capacity building enables communities that would otherwise be excluded to participate in the process, leading to better, and more just decisions. The Department of Energy (DOE) continues to be committed to promoting environmental justice and involving its stakeholders more directly in the planning and decision-making process for environmental cleanup. DOE's Environmental Management Program (EM) is in full support of this commitment. Through its environmental justice project, EMmore » provides communities with the capacity to effectively contribute to a complex technical decision-making process by furnishing access to computers, the Internet, training and technical assistance. DOE's Dr. Samuel P. Massie Chairs of Excellence Program (Massie Chairs) function as technical advisors to many of these community projects. The Massie Chairs consist of nationally and internationally recognized engineers and scientists from nine Historically Black Colleges and Universities (HBCUs) and one Hispanic Serving Institution (HIS). This paper will discuss capacity building initiatives in various jurisdictions.« less

  10. Proteomics tools reveal startlingly high amounts of oxytocin in plasma and serum

    NASA Astrophysics Data System (ADS)

    Brandtzaeg, Ole Kristian; Johnsen, Elin; Roberg-Larsen, Hanne; Seip, Knut Fredrik; Maclean, Evan L.; Gesquiere, Laurence R.; Leknes, Siri; Lundanes, Elsa; Wilson, Steven Ray

    2016-08-01

    The neuropeptide oxytocin (OT) is associated with a plethora of social behaviors, and is a key topic at the intersection of psychology and biology. However, tools for measuring OT are still not fully developed. We describe a robust nano liquid chromatography-mass spectrometry (nanoLC-MS) platform for measuring the total amount of OT in human plasma/serum. OT binds strongly to plasma proteins, but a reduction/alkylation (R/A) procedure breaks this bond, enabling ample detection of total OT. The method (R/A + robust nanoLC-MS) was used to determine total OT plasma/serum levels to startlingly high concentrations (high pg/mL-ng/mL). Similar results were obtained when combining R/A and ELISA. Compared to measuring free OT, measuring total OT can have advantages in e.g. biomarker studies.

  11. 12 CFR 516.30 - What information must I provide with my application?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... on OTS's web page at www.ots.treas.gov. (b) Captions and exhibits. You must caption the original... signatures on copies if you include a copy of the signed signature page or the copy otherwise indicates that...

  12. Assembling the Puzzle: Pathways of Oxytocin Signaling in the Brain.

    PubMed

    Grinevich, Valery; Knobloch-Bollmann, H Sophie; Eliava, Marina; Busnelli, Marta; Chini, Bice

    2016-02-01

    Oxytocin (OT) is a neuropeptide, which can be seen to be one of the molecules of the decade due to its profound prosocial effects in nonvertebrate and vertebrate species, including humans. Although OT can be detected in various physiological fluids (blood, saliva, urine, cerebrospinal fluid) and brain tissue, it is unclear whether peripheral and central OT releases match and synergize. Moreover, the pathways of OT delivery to brain regions involved in specific behaviors are far from clear. Here, we discuss the evolutionarily and ontogenetically determined pathways of OT delivery and OT signaling, which orchestrate activity of the mesolimbic social decision-making network. Furthermore, we speculate that both the alteration in OT delivery and OT receptor expression may cause behavioral abnormalities in patients afflicted with psychosocial diseases. Copyright © 2016 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.

  13. Maternal and paternal plasma, salivary, and urinary oxytocin and parent-infant synchrony: considering stress and affiliation components of human bonding.

    PubMed

    Feldman, Ruth; Gordon, Ilanit; Zagoory-Sharon, Orna

    2011-07-01

    Studies in mammals have implicated the neuropeptide oxytocin (OT) in processes of bond formation and stress modulation, yet the involvement of OT in human bonding throughout life remains poorly understood. We assessed OT in the plasma, saliva, and urine of 112 mothers and fathers interacting with their 4-6-month-old infants. Parent-infant interactions were micro-coded for parent and child's social behaviors and for the temporal coordination of their socio-affective cues. Parents were interviewed regarding their attachment to the infant and reported on bonding to own parents, romantic attachment, and parenting stress. Results indicated that OT in plasma (pOT) and saliva (sOT) were inter-related and were unrelated to OT in urine (uOT). pOT and sOT in mothers and fathers were associated with parent and child's social engagement, affect synchrony, and positive communicative sequences between parent and child. uOT was related to moments of interactive stress among mothers only, indexed by the co-occurrence of infant negative engagement and mother re-engagement attempts. pOT and sOT were associated with mothers' and fathers' attachment relationships throughout life: to own parents, partner, and infant, whereas uOT correlated with relationship anxiety and parenting stress among mothers only. Similar to other mammals, OT is involved in human attachment and contingent parenting. The dual role of OT in stress and affiliation underscores its complex involvement in processes of social bonding throughout life. © 2010 Blackwell Publishing Ltd.

  14. Adolescent oxytocin exposure causes persistent reductions in anxiety and alcohol consumption and enhances sociability in rats.

    PubMed

    Bowen, Michael T; Carson, Dean S; Spiro, Adena; Arnold, Jonathon C; McGregor, Iain S

    2011-01-01

    Previous studies have suggested that administration of oxytocin (OT) can have modulatory effects on social and anxiety-like behavior in mammals that may endure beyond the time of acute OT administration. The current study examined whether repeated administration of OT to male Wistar rats (n = 48) during a key developmental epoch (early adolescence) altered their physiology and behavior in later-life. Group housed rats were given intraperitoneal injections of either 1 mg/kg OT or vehicle during early adolescence (post natal-days [PND] 33-42). OT treatment caused a transient inhibition of body weight gain that recovered quickly after the cessation of treatment. At PND 50, the rats pre-treated with OT displayed less anxiety-like behavior on the emergence test, while at PND 55 they showed greater levels of social interaction. A subgroup of OT pre-treated rats examined at PND 63 showed a strong trend towards increased plasma OT levels, and also displayed significantly increased OT receptor mRNA in the hypothalamus. Rats pre-treated with OT and their controls showed similar induction of beer intake in daily 70 min test sessions (PND 63 onwards) in which the alcohol concentration of beer was gradually increased across days from 0.44% to 4.44%. However, when given ad libitum access to beer in their home cages from PND 72 onwards (early adulthood), consumption of beer but not water was significantly less in the OT pre-treated rats. A "booster" shot of OT (1 mg/kg) given after 25 days of ad libitum access to beer had a strong acute inhibitory effect on beer intake without affecting water intake. Overall these results suggest that exogenous OT administered during adolescence can have subtle yet enduring effects on anxiety, sociability and the motivation to consume alcohol. Such effects may reflect the inherent neuroplasticity of brain OT systems and a feed-forward effect whereby exogenous OT upregulates endogenous OT systems.

  15. Anatomy in Occupational Therapy Program Curriculum: Practitioners' Perspectives

    ERIC Educational Resources Information Center

    Schofield, Katherine Anne

    2014-01-01

    Anatomy education is undergoing significant transformation. It is unknown whether changes are in accordance with occupational therapy (OT) practice needs. The purpose of this pilot study was to survey OT clinicians to determine their perspectives on the value of anatomy in OT curricula, and anatomical knowledge required for practice. In addition…

  16. Predicting the Migration Rate of Dialkyl Organotins from PVC Pipe into Water

    EPA Science Inventory

    Organotins (OTs) are additives widely used as thermal and light stabilizers in polyvinyl chloride (PVC) plastics. OTs can leach into water flowing through PVC pipes. This work examines the leaching rates of two neurotoxic OTs, dimethyl tin (DMT) and dibutyl tin (DBT), from PVC pi...

  17. Practical Sequential Design Procedures for Submarine ASW Search Operational Testing: A Simulation Study

    DTIC Science & Technology

    1998-10-01

    The efficient design of a free play , 24 hour per day, operational test (OT) of an ASW search system remains a challenge to the OT community. It will...efficient, realistic, free play , 24 hour per day OT. The basic test control premise described here is to stop the test event if the time without a

  18. Maternal and Paternal Plasma, Salivary, and Urinary Oxytocin and Parent-Infant Synchrony: Considering Stress and Affiliation Components of Human Bonding

    ERIC Educational Resources Information Center

    Feldman, Ruth; Gordon, Ilanit; Zagoory-Sharon, Orna

    2011-01-01

    Studies in mammals have implicated the neuropeptide oxytocin (OT) in processes of bond formation and stress modulation, yet the involvement of OT in human bonding throughout life remains poorly understood. We assessed OT in the plasma, saliva, and urine of 112 mothers and fathers interacting with their 4-6-month-old infants. Parent-infant…

  19. Oxytocin reduces separation distress in piglets when given intranasally

    USDA-ARS?s Scientific Manuscript database

    Oxytocin (OT) is one of the neurobiological foundations of sociality. It acts as a neuropeptide in numerous social processes, from ultra-social to anti-social behaviors. Evidence supports a role for OT in social support, possibly by attenuating separation distress. Nonetheless, research on OT is lac...

  20. Entry-Level Doctorate for Occupational Therapists: An Assessment of Attitudes of Occupational Therapists and Occupational Therapy Assistants

    ERIC Educational Resources Information Center

    McCombie, Randy P.

    2016-01-01

    Purpose: The purpose of this study was to survey occupational therapists (OTs) and occupational therapy assistants (OTAs) on their attitudes toward a possible move to the entry-level doctorate as the mandated singular point of entry into the profession for OTs. Method: The study surveyed a random sample of 600 OTs and 600 OTAs from two…

  1. 12 CFR Appendix A to Part 563e - Ratings

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... to improve. The OTS rates a savings association's lending performance “needs to improve” if, in... OTS rates a savings association's investment performance “needs to improve” if, in general, it.... The OTS rates a savings association's service performance “needs to improve” if, in general, the...

  2. Ontogenesis of oxytocin pathways in the mammalian brain: late maturation and psychosocial disorders

    PubMed Central

    Grinevich, Valery; Desarménien, Michel G.; Chini, Bice; Tauber, Maithé; Muscatelli, Françoise

    2014-01-01

    Oxytocin (OT), the main neuropeptide of sociality, is expressed in neurons exclusively localized in the hypothalamus. During the last decade, a plethora of neuroendocrine, metabolic, autonomic and behavioral effects of OT has been reported. In the urgency to find treatments to syndromes as invalidating as autism, many clinical trials have been launched in which OT is administered to patients, including adolescents and children. However, the impact of OT on the developing brain and in particular on the embryonic and early postnatal maturation of OT neurons, has been only poorly investigated. In the present review we summarize available (although limited) literature on general features of ontogenetic transformation of the OT system, including determination, migration and differentiation of OT neurons. Next, we discuss trajectories of OT receptors (OTR) in the perinatal period. Furthermore, we provide evidence that early alterations, from birth, in the central OT system lead to severe neurodevelopmental diseases such as feeding deficit in infancy and severe defects in social behavior in adulthood, as described in Prader-Willi syndrome (PWS). Our review intends to propose a hypothesis about developmental dynamics of central OT pathways, which are essential for survival right after birth and for the acquisition of social skills later on. A better understanding of the embryonic and early postnatal maturation of the OT system may lead to better OT-based treatments in PWS or autism. PMID:25767437

  3. Tests of Several Model Nacelle-Propeller Arrangements in Front of a Wing

    DTIC Science & Technology

    1939-09-01

    uso in sub- sequent annl7scs 1 similar tosts vera made ot.tho wine alone. A second series ot tosts vas made ot each combination with the propeller...tained from tho toata of nacelles alo ~o (reference 2). ----------··-· ····---- ___ , ____ . • 14 !he maximum value of ~· has alread7 been shown to be

  4. Sexual experience increases oxytocin receptor gene expression and protein in the medial preoptic area of the male rat.

    PubMed

    Gil, Mario; Bhatt, Renu; Picotte, Katie B; Hull, Elaine M

    2013-09-01

    Oxytocin (OT) promotes social and reproductive behaviors in mammals, and OT deficits may be linked to disordered social behaviors like autism and severe anxiety. Male rat sexual behavior is an excellent model for OT regulation of behavior, as its pattern and neural substrates are well characterized. We previously reported that OT microinjected into the medial preoptic area (MPOA), a major integrative site for male sexual behavior, facilitates copulation in sexually experienced male rats, whereas intra-MPOA injection of an OT antagonist (OTA) inhibits copulation. In the present studies, copulation on the day of sacrifice stimulated OTR mRNA expression in the MPOA, irrespective of previous sexual experience, with the highest levels observed in first-time copulators. In addition, sexually experienced males had higher levels of OTR protein in the MPOA than sexually naïve males and first-time copulators. Finally, intra-MPOA injection of OT facilitated mating in sexually naive males. Others have reported a positive correlation between OT mRNA levels and male sexual behavior. Our studies show that OT in the MPOA facilitates mating in both sexually naive and experienced males, some of the behavioral effects of OT are mediated by the OTR, and sexual experience is associated with increased OTR expression in the MPOA. Taken together, these data suggest a reciprocal interaction between central OT and behavior, in which OT facilitates copulation and copulation stimulates the OT/OTR system in the brain. Copyright © 2013 Elsevier Ltd. All rights reserved.

  5. Job Choice and Personality: A Profile of Michigan Occupational and Physical Therapists.

    ERIC Educational Resources Information Center

    Lysack, Catherine; McNevin, Nancy; Dunleavy, Kim

    2001-01-01

    A Keirsey-Bates personality inventory was completed by 128 occupational therapists (OTs) and 166 physical therapists (PTs). PTs were far more likely to have sensing-judging temperament; OTs were predominantly sensing-perceiving or intuitive-feeling. The five most common OT types accounted for only 19% of PTs and the five most common PT types for…

  6. Central oxytocin receptor stimulation attenuates the orexigenic effects of butorphanol tartrate.

    PubMed

    Olszewski, Pawel K; Klockars, Oscar A; Klockars, Anica; Levine, Allen S

    2016-09-28

    Butorphanol tartrate (BT), a mixed µ/κ/δ opioid receptor agonist, is one of the most potent orexigens known to date. The central mechanisms through which BT causes hyperphagia are largely unknown. Interestingly, BT suppresses meal-end activation of neurons synthesizing anorexigenic neuropeptide, oxytocin (OT), which suggests that BT promotes hyperphagia by silencing OT-derived satiety signaling. As OT terminates consumption by acting by distinct hindbrain and forebrain circuits, we investigated whether stimulation of the OT receptor in the forebrain or the hindbrain [through lateral ventricular (LV) and fourth ventricular (4V) OT injections] leads to termination of food intake induced by BT. We established effective doses of BT on chow intake in ad-libitum-fed and overnight-deprived rats as well as effective doses of LV and 4V OT in deprived animals. Then, we determined doses of LV and 4V OT that reduce hyperphagia produced by BT in sated and deprived rats. Finally, we assessed whether OT's effects on BT-induced feeding can be suppressed by an OT receptor antagonist. 4 mg/kg BT increased intake in ad-libitum-fed and overnight-deprived rats, whereas LV and 4V OT at 1 μg caused a decrease in deprived rats. BT-induced chow intake in hungry and sated animals was suppressed by a very low, 0.1 μg dose of 4V OT, whereas 1 μg OT was effective LV. The effect of OT was attenuated by OT receptor antagonist, L-368 899. Reduced activity of the OT circuit, especially its hindbrain component, is a critical factor in shaping the magnitude of consumption in response to BT treatment.

  7. Oxytocin--its role in male reproduction and new potential therapeutic uses.

    PubMed

    Thackare, Hemlata; Nicholson, Helen D; Whittington, Kate

    2006-01-01

    Oxytocin (OT) is traditionally thought of as a "female" neurohypophysis hormone due to its role in parturition and milk ejection. However, OT is recognized as having endocrine and paracrine roles in male reproduction. At ejaculation, a burst of OT is released from the neurohypophysis into the systemic circulation and stimulates contractions of the reproductive tract aiding sperm release. There is conclusive evidence that OT is synthesized within the mammalian testis, epididymis and prostate and the presence of OT receptors (OTRs) through the reproductive tract supports a local action for this peptide. OT has a paracrine role in stimulating contractility of the seminiferous tubules, epididymis and the prostate gland. Interestingly, OT has also been shown to modulate androgen levels in these tissues via stimulation of the conversion of testosterone to dihydrotestostone (DHT) by 5alpha-reductase. The elucidation of OT's role in male reproduction has suggested a number of potential therapeutic uses for this hormone. Exogenous administration of OT has, in some cases, been shown to increase the numbers of ejaculated sperm, possibly by stimulating contractions of the reproductive tract and thus aiding sperm passage. Within the prostate, OT has been shown to affect gland growth both directly and via its interaction with androgen metabolism. Prostate pathologies due to unregulated cell proliferation/growth, such as benign prostatic hyperplasia and cancer, are unfortunately very common and few effective treatments are available. Greater understanding of paracrine growth mediators, such as OT, is likely to provide new mechanisms for treating such pathologies.

  8. Oxytocin's role in anxiety: a critical appraisal.

    PubMed

    MacDonald, Kai; Feifel, David

    2014-09-11

    A growing literature suggests that the oxytocin (OT) system may play a role in human anxiety states, anxiety-related traits, and moreover, that this system may be a target for the development of novel anxiolytic treatments. However, studies of OT's acute and chronic effects on various aspects of anxiety have produced mixed results. In this forward-looking review, we discuss the myriad phenomena to which the term "anxiety" is applied in the OT literature and the problem this presents developing a coherent picture of OT's role in anxiety. We then survey several different fields of research that support the role of the OT system in human anxiety, including evolutionary perspectives, translational and neuroimaging research, genetic studies, and clinical trials of intranasal OT. As an outgrowth of this data, we propose a "bowtie" model of OT's role at the interface of social attachment and anxiety. We next direct attention to understudied brain regions and neural circuits which may be important to study in OT experiments in humans anxiety disorders. Finally, we conclude by proposing questions and priorities for studying both the clinical potential of OT in anxiety, as well as mechanisms that may underlie this potential. Crucially, these priorities include targeted proof-of-concept clinical trials of IN OT in certain anxiety disorders, including investigations of individual moderators of OT's anxiolytic effects (i.e. sex, genetic factors, and early experience). This article is part of a Special Issue entitled Oxytocin and Social Behav. Copyright © 2014 Elsevier B.V. All rights reserved.

  9. Oxytocin and cardioprotection in diabetes and obesity.

    PubMed

    Jankowski, Marek; Broderick, Tom L; Gutkowska, Jolanta

    2016-06-07

    Oxytocin (OT) emerges as a drug for the treatment of diabetes and obesity. The entire OT system is synthesized in the rat and human heart. The direct myocardial infusion with OT into an ischemic or failing heart has the potential to elicit a variety of cardioprotective effects. OT treatment attenuates cardiomyocyte (CMs) death induced by ischemia-reperfusion by activating pro-survival pathways within injured CMs in vivo and in isolated cells. OT treatment reduces cardiac apoptosis, fibrosis, and hypertrophy. The OT/OT receptor (OTR) system is downregulated in the db/db mouse model of type 2 diabetes which develops genetic diabetic cardiomyopathy (DC) similar to human disease. We have shown that chronic OT treatment prevents the development of DC in the db/db mouse. In addition, OT stimulates glucose uptake in both cardiac stem cells and CMs, and increases cell resistance to diabetic conditions. OT may help replace lost CMs by stimulating the in situ differentiation of cardiac stem cells into functional mature CMs. Lastly, adult stem cells amenable for transplantation such as MSCs could be preconditioned with OT ex vivo and implanted into the injured heart to aid in tissue regeneration through direct differentiation, secretion of protective and cardiomyogenic factors and/or their fusion with injured CMs.

  10. Oxytocin and Interpersonal Relationships.

    PubMed

    Patin, Alexandra; Scheele, Dirk; Hurlemann, Rene

    2017-08-16

    The neuropeptide oxytocin (OT) has emerged as a potent modulator of diverse aspects of interpersonal relationships. OT appears to work in close interaction with several other neurotransmitter networks, including the dopaminergic reward circuit, and to be dependent on sex-specific hormonal influences. In this chapter, we focus on four main domains of OT and interpersonal relationships, including (1) the protective effect of OT on an individual's ability to withstand stress (i.e., stress buffering), (2) the effect of OT on emotion recognition and empathy, (3) OT's ability to enhance social synchrony and cooperation among individuals, and (4) the effect of OT on an individual's perception of social touch. We then illustrate the connection between OT and loneliness while grieving the loss of a loved one. We finish by discussing the clinical potential of OT, focusing on its potential role as an adjunct to psychotherapy, its enhancement through sex-specific hormonal influences, and the difficulties that present themselves when considering OT as a therapy. Overall, we argue that OT continues to hold strong therapeutic promise, but that it is strongly dependent on internal and external influences, for instance the patient's personal past experiences and interaction with the therapist in order to provide the best possible therapy.

  11. Oxytocin and vasopressin flatten dominance hierarchy and enhance behavioral synchrony in part via anterior cingulate cortex.

    PubMed

    Jiang, Yaoguang; Platt, Michael L

    2018-05-29

    The neuropeptides oxytocin (OT) and arginine vasopressin (AVP) influence social functions in many mammals. In humans and rhesus macaques, OT delivered intranasally can promote prosocial behavior in certain contexts. Yet the precise neural mechanisms mediating these behavioral effects remain unclear. Here we show that treating a group of male macaque monkeys intranasally with aerosolized OT relaxes their spontaneous social interactions with other monkeys. OT reduces differences in social behavior between dominant and subordinate monkeys, thereby flattening the status hierarchy. OT also increases behavioral synchrony within a pair. Intranasal delivery of aerosolized AVP reproduces the effects of OT with greater efficacy. Remarkably, all behavioral effects are replicated when OT or AVP is injected focally into the anterior cingulate gyrus (ACCg), a brain area linked to empathy and other-regarding behavior. ACCg lacks OT receptors but is rich in AVP receptors, suggesting exogenous OT may shape social behavior, in part, via nonspecific binding. Notably, OT and AVP alter behaviors of both the treated monkey and his untreated partner, consistent with enhanced feedback through reciprocal social interactions. These findings bear important implications for use of OT in both basic research and as a therapy for social impairments in neurodevelopmental disorders.

  12. Oxytocin as a novel therapeutic option for type I diabetes and diabetic osteopathy.

    PubMed

    Elabd, S K; Sabry, I; Mohasseb, M; Algendy, A

    2014-04-01

    The aim of the present study was to highlight the newly discovered metabolic role of oxytocin (OT) in the type I diabetic rats. Previous studies have demonstrated that OT has a beneficial role on bone physiology and therefore, the OT effect on the diabetic osteopathy will be assessed as well. Induction of the type I diabetes was carried out by an intraperitoneal injection of 60 mg/kg body weight of streptozotocin. The metabolic role of OT on diabetic rats after OT treatment with intramuscular injection of 40 µIU/kg body weight for 6 weeks was assessed. Histological and ultrastructural studies of rat pancreas samples, before and after the OT injection, were performed and compared with the obtained physiological results. Oxytocin treatment had positive metabolic effects in diabetic rats. This is based on the change in glucose metabolism, lipid profile, and insulin sensitivity in experimental animals. In addition, OT treatment showed histological regenerative changes of pancreatic islet cells of diabetic rats. Moreover, OT administration showed that it has an anabolic effect on the bone biology. The results suggest that activation of the oxytocin receptor (OTR) pathway by infusion of OT, OT analogs, or OT agonists may represent a promising approach for the treatment of diabetes and some of its complications, including diabetic osteopathy.

  13. CSF and blood oxytocin concentration changes following intranasal delivery in macaque.

    PubMed

    Dal Monte, Olga; Noble, Pamela L; Turchi, Janita; Cummins, Alex; Averbeck, Bruno B

    2014-01-01

    Oxytocin (OT) in the central nervous system (CNS) influences social cognition and behavior, making it a candidate for treating clinical disorders such as schizophrenia and autism. Intranasal administration has been proposed as a possible route of delivery to the CNS for molecules like OT. While intranasal administration of OT influences social cognition and behavior, it is not well established whether this is an effective means for delivering OT to CNS targets. We administered OT or its vehicle (saline) to 15 primates (Macaca mulatta), using either intranasal spray or a nebulizer, and measured OT concentration changes in the cerebral spinal fluid (CSF) and in blood. All subjects received both delivery methods and both drug conditions. Baseline samples of blood and CSF were taken immediately before drug administration. Blood was collected every 10 minutes after administration for 40 minutes and CSF was collected once post-delivery, at the 40 minutes time point. We found that intranasal administration of exogenous OT increased concentrations in both CSF and plasma compared to saline. Both delivery methods resulted in similar elevations of OT concentration in CSF, while the changes in plasma OT concentration were greater after nasal spray compared to nebulizer. In conclusion our study provides evidence that both nebulizer and nasal spray OT administration can elevate CSF OT levels.

  14. Effect of different doses of oxytocin on cardiac electrophysiology and arrhythmias induced by ischemia.

    PubMed

    Houshmand, Fariba; Faghihi, Mahdieh; Imani, Alireza; Kheiri, Soleiman

    2017-01-01

    The onset of acute myocardial ischemia (MI) is accompanied by a rapid increase in electrical instability and often fatal ventricular arrhythmias. This study investigated that whether oxytocin (OT) can modulate ischemia-induced arrhythmias and considered relationships between the severity of arrhythmia and the electrocardiogram parameters during ischemia. OT (0.0001-1 μg) was administrated intraperitoneally 30 min before ischemia. To examine receptor involved, a selective OT-receptor antagonist, atosiban (ATO), was infused 10 min before OT. OT caused a significant and biphasic dose-dependent reduction in ectopic heart activity and arrhythmia score. OT doses that reduced ventricular arrhythmia elicited significant increase in QT interval. OT attenuated the electrophysiological changes associated with MI and there was significant direct relationship between QRS duration and arrhythmia score. ATO treatment reduced beneficial effects of OT on arrhythmogenesis. Nevertheless, ATO failed to alter OT effects on premature ventricular contractions. We assume that the ability of OT to modulate the electrical activity of the heart may play an important role in the antiarrhythmic actions of OT.

  15. Effect of different doses of oxytocin on cardiac electrophysiology and arrhythmias induced by ischemia

    PubMed Central

    Houshmand, Fariba; Faghihi, Mahdieh; Imani, Alireza; Kheiri, Soleiman

    2017-01-01

    The onset of acute myocardial ischemia (MI) is accompanied by a rapid increase in electrical instability and often fatal ventricular arrhythmias. This study investigated that whether oxytocin (OT) can modulate ischemia-induced arrhythmias and considered relationships between the severity of arrhythmia and the electrocardiogram parameters during ischemia. OT (0.0001–1 μg) was administrated intraperitoneally 30 min before ischemia. To examine receptor involved, a selective OT-receptor antagonist, atosiban (ATO), was infused 10 min before OT. OT caused a significant and biphasic dose-dependent reduction in ectopic heart activity and arrhythmia score. OT doses that reduced ventricular arrhythmia elicited significant increase in QT interval. OT attenuated the electrophysiological changes associated with MI and there was significant direct relationship between QRS duration and arrhythmia score. ATO treatment reduced beneficial effects of OT on arrhythmogenesis. Nevertheless, ATO failed to alter OT effects on premature ventricular contractions. We assume that the ability of OT to modulate the electrical activity of the heart may play an important role in the antiarrhythmic actions of OT. PMID:29184844

  16. Toxoplasma Serotype Is Associated With Development of Ocular Toxoplasmosis

    PubMed Central

    Shobab, Leila; Pleyer, Uwe; Johnsen, Joerdis; Metzner, Sylvia; James, Erick R.; Torun, N.; Fay, Michael P.; Liesenfeld, Oliver; Grigg, Michael E.

    2013-01-01

    Background. Worldwide, ocular toxoplasmosis (OT) is the principal cause of posterior uveitis, a severe, life-altering disease. A Toxoplasma gondii enzyme-linked immunoassay that detects strain-specific antibodies present in serum was used to correlate serotype with disease. Methods. Toxoplasma serotypes in consecutive serum samples from German uveitis patients with OT were compared with non-OT seropositive patients with noninfectious autoimmune posterior uveitis. OT patients were tested for association of parasite serotype with age, gender, location, clinical onset, size, visual acuity, or number of lesions (mean follow-up, 3.8 years) to determine association with recurrences. Results. A novel, nonreactive (NR) serotype was detected more frequently in serum samples of OT patients (50/114, 44%) than in non-OT patients (4/56, 7%) (odds ratio, 10.0; 95% confidence interval 3.4–40.8; P < .0001). Non-OT patients were predominantly infected with Type II strains (39/56; 70%), consistent with expected frequencies in Central Europe. Among OT patients, those with NR serotypes experienced more frequent recurrences (P = .037). Polymerase chain reaction detected parasite DNA in 8/60 OT aqueous humor specimens but failed to identify Type II strain alleles. Conclusions. Toxoplasma NR and Type II serotypes predominate in German OT patients. The NR serotype is associated with OT recurrences, underscoring the value of screening for management of disease. PMID:23878321

  17. Toxoplasma serotype is associated with development of ocular toxoplasmosis.

    PubMed

    Shobab, Leila; Pleyer, Uwe; Johnsen, Joerdis; Metzner, Sylvia; James, Erick R; Torun, N; Fay, Michael P; Liesenfeld, Oliver; Grigg, Michael E

    2013-11-01

    Worldwide, ocular toxoplasmosis (OT) is the principal cause of posterior uveitis, a severe, life-altering disease. A Toxoplasma gondii enzyme-linked immunoassay that detects strain-specific antibodies present in serum was used to correlate serotype with disease. Toxoplasma serotypes in consecutive serum samples from German uveitis patients with OT were compared with non-OT seropositive patients with noninfectious autoimmune posterior uveitis. OT patients were tested for association of parasite serotype with age, gender, location, clinical onset, size, visual acuity, or number of lesions (mean follow-up, 3.8 years) to determine association with recurrences. A novel, nonreactive (NR) serotype was detected more frequently in serum samples of OT patients (50/114, 44%) than in non-OT patients (4/56, 7%) (odds ratio, 10.0; 95% confidence interval 3.4-40.8; P < .0001). Non-OT patients were predominantly infected with Type II strains (39/56; 70%), consistent with expected frequencies in Central Europe. Among OT patients, those with NR serotypes experienced more frequent recurrences (P = .037). Polymerase chain reaction detected parasite DNA in 8/60 OT aqueous humor specimens but failed to identify Type II strain alleles. Toxoplasma NR and Type II serotypes predominate in German OT patients. The NR serotype is associated with OT recurrences, underscoring the value of screening for management of disease.

  18. Association of Oxytocin and Parental Prefrontal Activation during Reunion with Infant: A Functional Near-Infrared Spectroscopy Study.

    PubMed

    Ito, Jun; Fujiwara, Takeo; Monden, Yukifumi; Yamagata, Takanori; Ohira, Hideki

    2017-01-01

    Although previous studies have revealed the role of oxytocin (OT) in parental behavior, the role of OT has not been investigated through the direct assessment of prefrontal brain activation during parenting. By using functional near-infrared spectroscopy, we aimed to show the relationship between parental [maternal ( N  = 15) and paternal ( N  = 21)] OT levels and the activation of the prefrontal cortex (PFC), while holding their infants after separation. Baseline OT levels were measured in the subjects' saliva samples before the experiment. Prefrontal brain activation was assessed in participants sitting alone on a chair (i.e., separation from their infant for 120 s) and during the target period (i.e., holding their infant for 45 s), which was done in triplicate. The oxygen hemoglobin (oxy-Hb) dissociation curve significantly increased in 9 out of 22 channels on the PFC when maternal and paternal samples were combined. However, only the fathers showed a correlation between salivary OT and oxy-Hb signal. Furthermore, while holding their infants, high-OT fathers showed left hemispheric dominance compared to low-OT fathers, while high-OT mothers showed right hemispheric dominance compared to low-OT mothers. This study showed that fathers with high-OT levels showed neural activation with left hemispheric dominance, while holding their infants, suggesting that increase of OT level might activate paternal PFC related to parenting behavior, although the same is not true for mothers.

  19. Regulation of 5alpha-reductase isoforms by oxytocin in the rat ventral prostate.

    PubMed

    Assinder, S J; Johnson, C; King, K; Nicholson, H D

    2004-12-01

    Oxytocin (OT) is present in the male reproductive tract, where it is known to modulate contractility, cell growth, and steroidogenesis. Little is known about how OT regulates these processes. This study describes the localization of OT receptor in the rat ventral prostate and investigates if OT regulates gene expression and/or activity of 5alpha-reductase isoforms I and II. The ventral prostates of adult male Wistar rats were collected following daily sc administration of saline (control), OT, a specific OT antagonist or both OT plus antagonist for 3 d. Expression of the OT receptor was identified in the ventral prostate by RT-PCR and Western blot, and confirmed to be a single active binding site by radioreceptor assay. Immunohistochemistry localized the receptor to the epithelium of prostatic acini and to the stromal tissue. Real-time RT-PCR determined that OT treatment significantly reduced expression of 5alpha-reductase I but significantly increased 5alpha-reductase II expression in the ventral prostate. Activity of both isoforms of 5alpha-reductase was significantly increased by OT, resulting in increased concentration of prostatic dihydrotestosterone. In conclusion, OT is involved in regulating conversion of testosterone to the biologically active dihydrotestosterone in the rat ventral prostate. It does so by differential regulation of 5alpha-reductase isoforms I and II.

  20. Role of oxytocin in improving the welfare of farm animals - A review.

    PubMed

    Chen, Siyu; Sato, Shusuke

    2017-04-01

    Recently, increasing attention has been paid to the welfare of farm animals, which have been evaluated using behavioral and physiological measures. However, so far, the measures have almost always been used to estimate poor welfare. In this review, firstly we focus on how oxytocin (OT) relates to positive social behavior, pleasure, and stress tolerance, and second on which management factors stimulate OT release. OT induces maternal and affiliative behaviors and has an anti-stress effect. Further, OT is produced during enjoyable events, and has positive feedback on its own release as well. Therefore, to some extent, the relationship of OT to positive normal behavior was mutually beneficial-heightened OT concentration owing to comfortable rearing conditions induces positive social behavior, which in turn may increase OT concentration. Hence, studies on animal welfare should pay more attention to increasing comfort and the stress tolerance, rather than only focusing on when stress occurs in farm animals.

  1. Dissociating frontal regions that co-lateralize with different ventral occipitotemporal regions during word processing.

    PubMed

    Seghier, Mohamed L; Price, Cathy J

    2013-08-01

    The ventral occipitotemporal sulcus (vOT) sustains strong interactions with the inferior frontal cortex during word processing. Consequently, activation in both regions co-lateralize towards the same hemisphere in healthy subjects. Because the determinants of lateralisation differ across posterior, middle and anterior vOT subregions, we investigated whether lateralisation in different inferior frontal regions would co-vary with lateralisation in the three different vOT subregions. A whole brain analysis found that, during semantic decisions on written words, laterality covaried in (1) posterior vOT and the precentral gyrus; (2) middle vOT and the pars opercularis, pars triangularis, and supramarginal gyrus; and (3) anterior vOT and the pars orbitalis, middle frontal gyrus and thalamus. These findings increase the spatial resolution of our understanding of how vOT interacts with other brain areas during semantic categorisation on words. Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

  2. Vasopressin and Oxytocin Reduce Food Sharing Behavior in Male, but Not Female Marmosets in Family Groups

    PubMed Central

    Taylor, Jack H.; Intorre, Allison A.; French, Jeffrey A.

    2017-01-01

    Oxytocin (OT) is critical for lactation and maternal care, but OT and the related nonapeptide vasopressin are important for caregiving behaviors in fathers and alloparents as well. This experiment tested the effects of vasopressin and OT on food sharing in marmoset families. We treated caregivers (parents, siblings) with intranasal vasopressin, OT, or saline, and then paired them with the youngest marmoset in the family. Caregivers were given preferred food, and then observed for food sharing and aggressive behavior with young marmosets. OT reduced food sharing from male alloparents to youngest siblings, and fathers that received vasopressin refused to share food with their youngest offspring more often than when treated with OT. Vasopressin increased aggressive vocalizations directed toward potential food recipients in all classes of caregivers. These results indicate that vasopressin and OT do not always enhance prosocial behavior: modulation of food sharing depends on both sex and parental status. PMID:28798724

  3. Vasopressin and Oxytocin Reduce Food Sharing Behavior in Male, but Not Female Marmosets in Family Groups.

    PubMed

    Taylor, Jack H; Intorre, Allison A; French, Jeffrey A

    2017-01-01

    Oxytocin (OT) is critical for lactation and maternal care, but OT and the related nonapeptide vasopressin are important for caregiving behaviors in fathers and alloparents as well. This experiment tested the effects of vasopressin and OT on food sharing in marmoset families. We treated caregivers (parents, siblings) with intranasal vasopressin, OT, or saline, and then paired them with the youngest marmoset in the family. Caregivers were given preferred food, and then observed for food sharing and aggressive behavior with young marmosets. OT reduced food sharing from male alloparents to youngest siblings, and fathers that received vasopressin refused to share food with their youngest offspring more often than when treated with OT. Vasopressin increased aggressive vocalizations directed toward potential food recipients in all classes of caregivers. These results indicate that vasopressin and OT do not always enhance prosocial behavior: modulation of food sharing depends on both sex and parental status.

  4. Dissociating frontal regions that co-lateralize with different ventral occipitotemporal regions during word processing☆

    PubMed Central

    Seghier, Mohamed L.; Price, Cathy J.

    2013-01-01

    The ventral occipitotemporal sulcus (vOT) sustains strong interactions with the inferior frontal cortex during word processing. Consequently, activation in both regions co-lateralize towards the same hemisphere in healthy subjects. Because the determinants of lateralisation differ across posterior, middle and anterior vOT subregions, we investigated whether lateralisation in different inferior frontal regions would co-vary with lateralisation in the three different vOT subregions. A whole brain analysis found that, during semantic decisions on written words, laterality covaried in (1) posterior vOT and the precentral gyrus; (2) middle vOT and the pars opercularis, pars triangularis, and supramarginal gyrus; and (3) anterior vOT and the pars orbitalis, middle frontal gyrus and thalamus. These findings increase the spatial resolution of our understanding of how vOT interacts with other brain areas during semantic categorisation on words. PMID:23728081

  5. Therapy Use for Children With Developmental Conditions: Analysis of Colorado Medicaid Data.

    PubMed

    McManus, Beth M; Rapport, Mary Jane; Richardson, Zachary; Lindrooth, Richard

    2017-07-01

    To examine therapy use and spending for Medicaid-enrolled infants and toddlers with developmental conditions. Sample infants and toddlers had a diagnosis (eg, cerebral palsy) or developmental delay (DD). Colorado Children's Medicaid administrative outpatient therapy claims (2006-2008) were used to estimate differences, by condition type and number of comorbid chronic conditions (CCCs), of any physical therapy (PT)/occupational therapy (OT) and Medicaid PT/OT spending. The sample included 20 959 children. Children with at least 2 CCCs had higher odds of PT/OT than children with no CCC. Children with DD had 12-fold higher odds of having any PT/OT compared with children with diagnosis. Children with a DD and 2 CCCs had the highest PT/OT spending. Medicaid PT/OT use and spending are higher for children with more CCCs and those with DD because children with DD receive more specialized PT/OT.

  6. Effects of oxytocin administration on spirituality and emotional responses to meditation

    PubMed Central

    Way, Baldwin M.; Isgett, Suzannah F.; Fredrickson, Barbara L.

    2016-01-01

    The oxytocin (OT) system, critically involved in social bonding, may also impinge on spirituality, which is the belief in a meaningful life imbued with a sense of connection to a Higher Power and/or the world. Midlife male participants (N = 83) were randomly assigned to receive intranasal OT or placebo. In exploratory analyses, participants were also genotyped for polymorphisms in two genes critical for OT signaling, the oxytocin receptor gene (OXTR rs53576) and CD38 (rs6449182 and rs3796863). Results showed that intranasal OT increased self-reported spirituality on two separate measures and this effect remained significant a week later. It also boosted participants’ experience of specific positive emotions during meditation, at both explicit and implicit levels. Furthermore, the effect of OT on spirituality was moderated by OT-related genotypes. These results provide the first experimental evidence that spirituality, endorsed by millions worldwide, appears to be supported by OT. PMID:27317929

  7. Neuropeptide diversity and the regulation of social behavior in New World primates

    PubMed Central

    French, Jeffrey A.; Taylor, Jack H.; Mustoe, Aaryn C.; Cavanaugh, Jon

    2016-01-01

    Oxytocin (OT) and vasopressin (AVP) are important hypothalamic neuropeptides that regulate peripheral physiology, and have emerged as important modulators of brain function, particularly in the social realm. OT structure and the genes that ultimately determine structure are highly conserved among diverse eutherian mammals, but recent discoveries have identified surprising variability in OT and peptide structure in New World monkeys (NWM), with five new OT variants identified to date. This review explores these new findings in light of comparative OT/AVP ligand evolution, documents coevolutionary changes in the oxytocin and vasopressin receptors (OTR and V1aR), and highlights the distribution of neuropeptidergic neurons and receptors in the primate brain. Finally, the behavioral consequences of OT and AVP in regulating NWM sociality are summarized, demonstrating important neuromodulatory effects of these compounds and OT ligand-specific influences in certain social domains. PMID:27020799

  8. Body temperature and cardiac changes induced by peripherally administered oxytocin, vasopressin and the non-peptide oxytocin receptor agonist WAY 267,464: a biotelemetry study in rats

    PubMed Central

    Hicks, C; Ramos, L; Reekie, T; Misagh, G H; Narlawar, R; Kassiou, M; McGregor, I S

    2014-01-01

    Background and Purpose There is current interest in oxytocin (OT) as a possible therapeutic in psychiatric disorders. However, the usefulness of OT may be constrained by peripheral autonomic effects, which may involve an action at both OT and vasopressin V1A receptors. Here, we characterized the cardiovascular and thermoregulatory effects of OT, vasopressin (AVP) and the non-peptide OT receptor agonist WAY 267,464 in rats, and assessed the relative involvement of the OT and V1A receptors in these effects. Experimental Approach Biotelemetry in freely moving male Wistar rats was used to examine body temperature and heart rate after OT (0.01 – 1 mg kg−1; i.p.), AVP (0.001 – 0.1 mg kg−1; i.p.) or WAY 267,464 (10 and 100 mg kg−1; i.p.). The actions of the OT receptor antagonist Compound 25 (C25, 5 and 10 mg kg−1) and V1A receptor antagonist SR49059 (1 and 10 mg kg−1) were studied, as well as possible V1A receptor antagonist effects of WAY 267,464. Key Results OT and AVP dose-dependently reduced body temperature and heart rate. WAY 267,464 had similar, but more modest, effects. SR49059, but not C25, prevented the hypothermia and bradycardia induced by OT and AVP. WAY 267,464 (100 mg·kg−1) prevented the effects of OT, and to some extent AVP. Conclusions and Implications Peripherally administered OT and AVP have profound cardiovascular and thermoregulatory effects that appear to principally involve the V1A receptor rather than the OT receptor. Additionally, WAY 267,464 is not a simple OT receptor agonist, as it has functionally relevant V1A antagonist actions. PMID:24641248

  9. Body temperature and cardiac changes induced by peripherally administered oxytocin, vasopressin and the non-peptide oxytocin receptor agonist WAY 267,464: a biotelemetry study in rats.

    PubMed

    Hicks, C; Ramos, L; Reekie, T; Misagh, G H; Narlawar, R; Kassiou, M; McGregor, I S

    2014-06-01

    There is current interest in oxytocin (OT) as a possible therapeutic in psychiatric disorders. However, the usefulness of OT may be constrained by peripheral autonomic effects, which may involve an action at both OT and vasopressin V1A receptors. Here, we characterized the cardiovascular and thermoregulatory effects of OT, vasopressin (AVP) and the non-peptide OT receptor agonist WAY 267,464 in rats, and assessed the relative involvement of the OT and V1A receptors in these effects. Biotelemetry in freely moving male Wistar rats was used to examine body temperature and heart rate after OT (0.01 - 1 mg kg(-1); i.p.), AVP (0.001 - 0.1 mg kg(-1); i.p.) or WAY 267,464 (10 and 100 mg kg(-1); i.p.). The actions of the OT receptor antagonist Compound 25 (C25, 5 and 10 mg kg(-1)) and V1A receptor antagonist SR49059 (1 and 10 mg kg(-1)) were studied, as well as possible V1A receptor antagonist effects of WAY 267,464. OT and AVP dose-dependently reduced body temperature and heart rate. WAY 267,464 had similar, but more modest, effects. SR49059, but not C25, prevented the hypothermia and bradycardia induced by OT and AVP. WAY 267,464 (100 mg·kg(-1)) prevented the effects of OT, and to some extent AVP. Peripherally administered OT and AVP have profound cardiovascular and thermoregulatory effects that appear to principally involve the V1A receptor rather than the OT receptor. Additionally, WAY 267,464 is not a simple OT receptor agonist, as it has functionally relevant V1A antagonist actions. © 2014 The British Pharmacological Society.

  10. Neural Basis of Ventromedial Hypothalamic Oxytocin-Driven Decrease in Appetite.

    PubMed

    Klockars, Oscar A; Waas, Joseph R; Klockars, Anica; Levine, Allen S; Olszewski, Pawel K

    2017-12-16

    Oxytocin (OT) administration in the ventromedial hypothalamic nucleus (VMH) reduces chow intake. The nature of VMH OT's anorexigenic action remains unclear. Here we provide insight into neural mechanisms underlying VMH OT-driven anorexia by (a) identifying feeding-related brain sites activated by VMH OT injection; (b) measuring VMH OT receptor (OTr) mRNA changes in response to hunger and palatability; and (c) examining how VMH OT affects episodic sweet solution intake in sated and hungry rats. We established effective doses of VMH OT in deprivation-induced and scheduled feeding and determined whether an OT antagonist blocks the effect. Then, OT (or antagonist) was injected in the VMH of sated rats given episodically sucrose and saccharin solutions. OT was also injected in hungry animals offered simultaneously chow and sugar water. Brain activation after VMH OT was determined by Fos immunoreactivity (IR). OTr expression was established with rtPCR after chow deprivation or saccharin exposure. VMH OT decreased intake of chow and the effect was reversed by the antagonist, though the antagonist alone was not orexigenic. OT did not affect intakes of energy-dilute saccharin and sucrose solutions in sated or hungry rats. Fos IR was elevated in the VMH and energy balance-related paraventricular and arcuate nuclei, but not reward areas. VMH OTr expression was higher in hungry rats than in sated controls; saccharin intake had no effect. OT acting in the VMH decreases intake driven by energy not by palatability, and it stimulates activity of hypothalamic sites controlling energy balance. Copyright © 2017 IBRO. Published by Elsevier Ltd. All rights reserved.

  11. Operating theatre ventilation systems and microbial air contamination in total joint replacement surgery: results of the GISIO-ISChIA study.

    PubMed

    Agodi, A; Auxilia, F; Barchitta, M; Cristina, M L; D'Alessandro, D; Mura, I; Nobile, M; Pasquarella, C

    2015-07-01

    Recent studies have shown a higher rate of surgical site infections in hip prosthesis implantation using unidirectional airflow ventilation compared with turbulent ventilation. However, these studies did not measure the air microbial quality of operating theatres (OTs), and assumed it to be compliant with the recommended standards for this ventilation technique. To evaluate airborne microbial contamination in OTs during hip and knee replacement surgery, and compare the findings with values recommended for joint replacement surgery. Air samplings were performed in 28 OTs supplied with unidirectional, turbulent and mixed airflow ventilation. Samples were collected using passive sampling to determine the index of microbial air contamination (IMA). Active sampling was also performed in some of the OTs. The average number of people in the OT and the number of door openings during the sampling period were recorded. In total, 1228 elective prosthesis procedures (60.1% hip and 39.9% knee) were included in this study. Of passive samplings performed during surgical activity in unidirectional airflow ventilation OTs (U-OTs) and mixed airflow OTs (M-OTs), 58.9% and 87.6% had IMA values >2, respectively. Of samplings performed during surgical activity in turbulent airflow OTs (T-OTs) and in turbulent airflow OTs with the surgical team wearing Steri-Shield Turbo Helmets (TH-OTs), 8.6% and 60% had IMA values ≤ 2, respectively. Positive correlation was found between IMA values and the number of people in the OT and the number of door openings (P < 0.001). In addition, correlation was found between active and passive sampling (P < 0.001). These findings challenge the belief that unidirectional systems always provide acceptable airborne bacterial counts. Copyright © 2015 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

  12. Results of an investigation of jet plume effects on an 0.010-scale model (75-OTS) of the space shuttle integrated vehicle in the 9 x 7-foot leg of the NASA/Ames unitary wind tunnel (IA82B), volume 1. [an exhaust flow simulation

    NASA Technical Reports Server (NTRS)

    Hawthorne, P. J.

    1976-01-01

    The base pressure environment was investigated for the first and second stage mated vehicle in a supersonic flow field from Mach 1.55 through 2.20 with simulated rocket engine exhaust plumes. The pressure environment was investigated for the orbiter at various vent port locations at these same freestream conditions. The Mach number environment around the base of the model with rocket plumes simulated was examined. Data were obtained at angles of attack from -4 deg through +4 deg at zero yaw, and at yaw angles from -4 deg through +4 deg at zero angle of attack, with rocket plume sizes varying from smaller than nominal to much greater than nominal. Failed orbiter engine data were also obtained. Elevon hinge moments and wing panel load data were obtained during all runs. Photographs of the tested configurations are shown.

  13. Results of an investigation of jet plume effects on a 0.010-scale model (75-OTS) of the space shuttle integrated vehicle in the 8 x 7-foot leg of the NASA/Ames unitary wind tunnel (IA82C), volume 1. [(an exhaust flow simulation)

    NASA Technical Reports Server (NTRS)

    Hawthorne, P. J.

    1976-01-01

    The primary test objective was to define the base pressure environment of the first and second stage mated vehicle in a supersonic flow field from Mach 2.60 through 3.50 with simulated rocket engine exhaust plumes. The secondary objective was to obtain the pressure environment of the Orbiter at various vent port locations at these same freestream conditions. Data were obtained at angles of attack from -4 deg through +4 deg at zero yaw, and at yaw angles from -4 deg through +4 deg at zero angle of attack, with rocket plume sizes varying from smaller than nominal to much greater than nominal. Failed Orbiter engine data were also obtained. Elevon hinge moments and wing panel load data were obtained during all runs. Photographs of test equipment and tested configurations are shown.

  14. Results of tests OA26 and IA16 in the NASA/ARC 3.5-foot hypersonic wind tunnel on an 0.015-scale model (36-OTS) of the space shuttle configuration 140A/B to obtain pressures for venting analysis

    NASA Technical Reports Server (NTRS)

    Spangler, R. H.; Thornton, D. E.; Polek, T. E.

    1974-01-01

    Tests were conducted, from November 15 to December 4, 1973, to obtain surface pressure data on an 0.015-scale replica of the Space Shuttle Vehicle 4. Data were obtained at Mach numbers of 5.3, 7.4, and 10.3, to support the venting analysis for both launch and entry conditions. These tests were the final tests in a series covering a Mach number range from 0.6 to 10.3. The model was instrumented with pressure orifices in the vicinity of the cargo bay door hinge and parting lines, and on the side of the fuselage at the crew compartment, and below the orbital maneuvering system pods at the aft compartment. The model was tested at angles of attack and sideslip consistent with expected divergencies from the nominal trajectory.

  15. Results of a pressure loads investigation on a 0.030-scale model (47-OTS) of the integrated space shuttle vehicle configuration 5 in the NASA Ames Research Center 9 by 7 foot leg of the unitary plan wind tunnel (IA81B), volume 1

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1975-01-01

    The investigations of pressure distributions are presented for aeroloads analysis at Mach numbers from 1.55 through 2.5. Angles of attack and sideslip varied from -6 to +6 degrees. Photographs of wind tunnel models are shown.

  16. Results of tests in the NASA/LaRC 31-inch CFHT on an 0.010-scale model (32-OT) of the space shuttle configuration 3 to determine the RCS jet flowfield interaction effects on aerodynamic characteristics (IA60/0A105), volume 1

    NASA Technical Reports Server (NTRS)

    Thornton, D. E.

    1974-01-01

    Tests were conducted in the 31-inch continuous Flow Hypersonic Wind Tunnel to determine RCS jet interaction effect on the hypersonic aerodynamic and stability and control characteristics prior to RTLS abort separation. The model used was an 0.010-scale replica of the Space Shuttle Vehicle Configuration 3. Hypersonic stability data were obtained from tests at Mach 10.3 and dynamic pressure of 150 psf for the integrated Orbiter and external tank and the Orbiter alone. RCS modes of pitch, yaw, and roll at free flight dynamic pressure simulation of 7, 20, and 50 psf were investigated. The effects of speedbrake, bodyflap, elevon, and airleron deflections were also investigated.

  17. Results of tests in the NASA/LaRC 31-inch CFHT on an 0.010-scale model (32-OT) of the space shuttle configuration 3 to determine the RCS jet flowfield interaction effects on aerodynamic characteristics (IA60/0A105), volume 2

    NASA Technical Reports Server (NTRS)

    Thornton, D. E.

    1974-01-01

    Tests were conducted in the NASA Langley Research Center 31-inch continuous flow hypersonic wind tunnel from 14 February to 22 February 1974, to determine RCS jet interaction effect on the hypersonic aerodynamic and stability and control characteristics prior to RTLS abort separation. The model used was an 0.010-scale replica of the space shuttle vehicle configuration 3. Hypersonic stability data were obtained from tests at Mach 10.3 and dynamic pressure of 150 psf for the intergrated orbiter and external tank and the orbiter alone. RCS modes of pitch, yaw, and roll at free flight dynamic pressure simulation of 7, 20, and 50 psf were investigated. The effects of speedbrake, bodyflap, elevon, and aileron deflections were also investigated.

  18. Results of a jet plume effects test on Rockwell International integrated space shuttle vehicle using a vehicle 5 configuration 0.02-scale model (88-OTS) in the 11 by 11 foot leg of the NASA/Ames Research Center unitary plan wind tunnel (IA19), volume 1

    NASA Technical Reports Server (NTRS)

    Nichols, M. E.

    1975-01-01

    Results are presented of jet plume effects test IA19 using a vehicle 5 configuration integrated space shuttle vehicle 0.02-scale model in the NASA/Ames Research Center 11 x 11-foot leg of the unitary plan wind tunnel. The jet plume power effects on the integrated vehicle static pressure distribution were determined along with elevon, main propulsion system nozzle, and solid rocket booster nozzle effectiveness and elevon hinge moments.

  19. Results of tests in the NASA/LARC 31-inch CFHT on an 0.010-scale model (32-OT) of the space shuttle configuration 3 to determine the RCS jet flowfield interaction effects on aerodynamic characteristics (IA60/OA105), volume 1

    NASA Technical Reports Server (NTRS)

    Thornton, D. E.

    1974-01-01

    Tests were conducted in the NASA Langley Research Center 31-inch continuous Flow Hypersonic Wind Tunnel to determine RCS jet interaction effect on the hypersonic aerodynamic and stability and control characteristics prior to return to launch site (RTLS) abort separation. The model used was an 0.010-scale replica of the Space Shuttle Vehicle Configuration 3. Hypersonic stability data were obtained from tests at Mach 10.3 and dynamic pressure of 150 psf for the integrated Orbiter and external tank and the Orbiter alone. RCS modes of pitch, yaw, and roll at free flight dynamic pressure simulation of 7, 20, and 50 psf were investigated. The effects of speedbrake, bodyflap, elevon, and aileron deflections were also investigated.

  20. Results of a Pressure Loads Investigation on a 0.030-scale Model (47-OTS) of the Integrated Space Shuttle Vehicle Configuration 5 in the NASA Ames Research Center 11 by 11 Foot Leg of the Unitary Plan Wind Tunnel (IA81A), Volume 1

    NASA Technical Reports Server (NTRS)

    Chee, E.

    1975-01-01

    Results of wind tunnel tests on a 0.030-scale model of the integrated space shuttle vehicle configuration 5 are presented. Testing was conducted in the NASA Ames Research Center 11 x 11 foot leg of the Unitary Plan Wind Tunnel to investigate pressure distributions for airloads analyses at Mach numbers from 0.9 through 1.4. Angles of attack and sideslip were varied from -6 to +6 degrees.

  1. Use, trends, and impacts of nurse overtime in New York hospitals, 1995--2000

    NASA Astrophysics Data System (ADS)

    Berney, Barbara L.

    Over the last several years, nurses and their advocates have expressed concern about heavy use of overtime (OT) in hospitals and claimed that it undermines the quality of nursing care. Some hospital managers view OT as an important staffing and scheduling tool. This study examines trends in the use of nurse overtime and its effects on several nurse-sensitive patient outcomes. Previous studies have demonstrated a relationship between several patient outcomes and nurse staffing levels, but little is known about the impact of nurse overtime on patient outcomes. Using staffing and discharge data covering 1995 to 2000 from 160 acute general hospitals in New York State, this study examines hospital characteristics that are associated with the use of OT. These characteristics include size, location, RN unionization, hospital ownership and teaching status. The study examines trends in the use of OT. Finally, it uses multivariate regression to analyze the relationship between OT and the rates of six nurse-sensitive patient outcomes and mortality. Significant differences were observed in the use of OT by hospital ownership and by union status. Government hospitals used less OT than non-government hospitals. Non-union hospitals used slightly less OT than hospitals with unionized nurses. As total RN hours and straight (non-OT) hours per acute inpatient day increased, OT decreased. OT use as a percent of total inpatient RN hours increased significantly over the study period from 3.9% to 5.5%. Trends varied by hospital characteristic. This dissertation finds little evidence for an association between nurse OT and patient outcomes. One reason may be that the administrative data used track OT use and adverse events for whole years. Since OT use is episodic, data that examine the occurrence of adverse events during periods of heavy nurse overtime may be more sensitive to the impact of OT. That hospitals vary dramatically in their OT use and that some categories of hospitals (e

  2. Comparison Between GOES-12 Overshooting-Top Detections, WSR-88D Radar Reflectivity, and Severe Storm Reports

    NASA Technical Reports Server (NTRS)

    Dworak, Richard; Bedka, Kristopher; Brunner, Jason; Feltz, Wayne

    2012-01-01

    Studies have found that convective storms with overshooting-top (OT) signatures in weather satellite imagery are often associated with hazardous weather, such as heavy rainfall, tornadoes, damaging winds, and large hail. An objective satellite-based OT detection product has been developed using 11-micrometer infrared window (IRW) channel brightness temperatures (BTs) for the upcoming R series of the Geostationary Operational Environmental Satellite (GOES-R) Advanced Baseline Imager. In this study, this method is applied to GOES-12 IRW data and the OT detections are compared with radar data, severe storm reports, and severe weather warnings over the eastern United States. The goals of this study are to 1) improve forecaster understanding of satellite OT signatures relative to commonly available radar products, 2) assess OT detection product accuracy, and 3) evaluate the utility of an OT detection product for diagnosing hazardous convective storms. The coevolution of radar-derived products and satellite OT signatures indicates that an OT often corresponds with the highest radar echo top and reflectivity maximum aloft. Validation of OT detections relative to composite reflectivity indicates an algorithm false-alarm ratio of 16%, with OTs within the coldest IRW BT range (less than 200 K) being the most accurate. A significant IRW BT minimum typically present with an OT is more often associated with heavy precipitation than a region with a spatially uniform BT. Severe weather was often associated with OT detections during the warm season (April September) and over the southern United States. The severe weather to OT relationship increased by 15% when GOES operated in rapid-scan mode, showing the importance of high temporal resolution for observing and detecting rapidly evolving cloud-top features. Comparison of the earliest OT detection associated with a severe weather report showed that 75% of the cases occur before severe weather and that 42% of collocated severe

  3. Oxytocin alters cell fate selection of rat neural progenitor cells in vitro

    PubMed Central

    Kannappan, Ramaswamy; Xu, Zhiqiang; Martino, Audrey; Friese, Matthew B.; Boyd, Justin D.; Crosby, Gregory; Culley, Deborah J.

    2018-01-01

    Synthetic oxytocin (sOT) is widely used during labor, yet little is known about its effects on fetal brain development despite evidence that it reaches the fetal circulation. Here, we tested the hypothesis that sOT would affect early neurodevelopment by investigating its effects on neural progenitor cells (NPC) from embryonic day 14 rat pups. NPCs expressed the oxytocin receptor (OXTR), which was downregulated by 45% upon prolonged treatment with sOT. Next, we examined the effects of sOT on NPC death, apoptosis, proliferation, and differentiation using antibodies to NeuN (neurons), Olig2 (oligodendrocytes), and GFAP (astrocytes). Treated NPCs were analysed with unbiased high-throughput immunocytochemistry. Neither 6 nor 24 h exposure to 100 pM or 100 nM sOT had an effect on viability as assessed by PI or CC-3 immunocytochemistry. Similarly, sOT had negligible effect on NPC proliferation, except that the overall rate of NPC proliferation was higher in the 24 h compared to the 6 h group regardless of sOT exposure. The most significant finding was that sOT exposure caused NPCs to select a predominantly neuronal lineage, along with a concomitant decrease in glial cells. Collectively, our data suggest that perinatal exposure to sOT can have neurodevelopmental consequences for the fetus, and support the need for in vivo anatomical and behavioral studies in offspring exposed to sOT in utero. PMID:29346405

  4. Oxytocin alters cell fate selection of rat neural progenitor cells in vitro.

    PubMed

    Palanisamy, Arvind; Kannappan, Ramaswamy; Xu, Zhiqiang; Martino, Audrey; Friese, Matthew B; Boyd, Justin D; Crosby, Gregory; Culley, Deborah J

    2018-01-01

    Synthetic oxytocin (sOT) is widely used during labor, yet little is known about its effects on fetal brain development despite evidence that it reaches the fetal circulation. Here, we tested the hypothesis that sOT would affect early neurodevelopment by investigating its effects on neural progenitor cells (NPC) from embryonic day 14 rat pups. NPCs expressed the oxytocin receptor (OXTR), which was downregulated by 45% upon prolonged treatment with sOT. Next, we examined the effects of sOT on NPC death, apoptosis, proliferation, and differentiation using antibodies to NeuN (neurons), Olig2 (oligodendrocytes), and GFAP (astrocytes). Treated NPCs were analysed with unbiased high-throughput immunocytochemistry. Neither 6 nor 24 h exposure to 100 pM or 100 nM sOT had an effect on viability as assessed by PI or CC-3 immunocytochemistry. Similarly, sOT had negligible effect on NPC proliferation, except that the overall rate of NPC proliferation was higher in the 24 h compared to the 6 h group regardless of sOT exposure. The most significant finding was that sOT exposure caused NPCs to select a predominantly neuronal lineage, along with a concomitant decrease in glial cells. Collectively, our data suggest that perinatal exposure to sOT can have neurodevelopmental consequences for the fetus, and support the need for in vivo anatomical and behavioral studies in offspring exposed to sOT in utero.

  5. Effects of intranasal oxytocin on social anxiety in males with fragile X syndrome.

    PubMed

    Hall, Scott S; Lightbody, Amy A; McCarthy, Brigid E; Parker, Karen J; Reiss, Allan L

    2012-04-01

    Fragile X syndrome (FXS) is a rare inherited genetic disorder causing severe intellectual disability and autistic-like symptoms. Individuals with FXS, males in particular, often exhibit extreme eye gaze avoidance and hyperarousal when they encounter stressful social situations. We investigated whether oxytocin (OT), a hormone with prosocial and anxiolytic effects, could alleviate symptoms of social anxiety in this population. A randomized double-blind placebo-controlled single-dose trial was performed with intranasal administration of placebo, 24 IU OT and 48 IU OT. Measures of eye gaze frequency, heart rate, respiratory sinus arrhythmia (RSA), heart rate variability (HRV) and salivary cortisol were obtained during a structured social challenge conducted 50 min following OT administration. Ten low-functioning males with FXS (aged 13-28 years) traveled to Stanford for the initial visit: 8 completed the study. Eye gaze frequency improved significantly in response to the 24 IU OT dose and salivary cortisol levels decreased significantly in response to the 48 IU OT dose. There was no effect of OT on heart rate, RSA or HRV although individual plots of the heart rate data suggested that OT increased heart rate in some participants and decreased heart rate in others. These findings suggest that intranasal administration of OT may ameliorate some symptoms of social anxiety in patients with FXS. Further double-blind placebo-controlled studies of OT, conducted in combination with behavioral treatment programs, may be warranted. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. Orthodontic treatment for disabled children: a survey of parents' attitudes and overall satisfaction.

    PubMed

    Abeleira, María Teresa; Pazos, Elisabeth; Ramos, Isabel; Outumuro, Mercedes; Limeres, Jacobo; Seoane-Romero, Juan; Diniz, Marcio; Diz, Pedro

    2014-08-05

    Many patients with disability require orthodontic treatment (OT) to achieve adequate oral function and aesthetic appearance. The cooperation of disabled patients and of their parents is central to the success of OT, as treatment can involve ethical dilemmas. The aim of this study was to analyze the motivation, expectations and overall satisfaction with OT among parents of patients with disabilities. The parents of 60 disabled Spanish children with physical, mental and/or sensory impairment undergoing OT were surveyed on attitudes to OT and level of satisfaction with the outcomes. The survey consisted of 23 questions in 4 sections: attitude and adaptation, benefits, adverse effects, and level of satisfaction after completion of OT. A control group formed of the parents of 60 healthy children undergoing OT at the same institution were also surveyed. Parents of disabled children undergoing OT showed a high level of motivation and they are willing to collaborate in oral hygiene procedures. Adaptation to the removable appliances was poorer in disabled children but adaptation to fixed appliances was excellent. OT can provide a marked improvement in quality of life, social relationships and oral functionality in disabled children. Among parents of disabled children undergoing OT, the perceived level of overall satisfaction was very high and expectations were often exceeded.

  7. Orthodontic treatment for disabled children: a survey of parents’ attitudes and overall satisfaction

    PubMed Central

    2014-01-01

    Background Many patients with disability require orthodontic treatment (OT) to achieve adequate oral function and aesthetic appearance. The cooperation of disabled patients and of their parents is central to the success of OT, as treatment can involve ethical dilemmas. The aim of this study was to analyze the motivation, expectations and overall satisfaction with OT among parents of patients with disabilities. Methods The parents of 60 disabled Spanish children with physical, mental and/or sensory impairment undergoing OT were surveyed on attitudes to OT and level of satisfaction with the outcomes. The survey consisted of 23 questions in 4 sections: attitude and adaptation, benefits, adverse effects, and level of satisfaction after completion of OT. A control group formed of the parents of 60 healthy children undergoing OT at the same institution were also surveyed. Results Parents of disabled children undergoing OT showed a high level of motivation and they are willing to collaborate in oral hygiene procedures. Adaptation to the removable appliances was poorer in disabled children but adaptation to fixed appliances was excellent. OT can provide a marked improvement in quality of life, social relationships and oral functionality in disabled children. Conclusions Among parents of disabled children undergoing OT, the perceived level of overall satisfaction was very high and expectations were often exceeded. PMID:25096027

  8. Organotin Exposure and Vertebrate Reproduction: A Review

    PubMed Central

    de Araújo, Julia Fernandez Puñal; Podratz, Priscila Lang; Merlo, Eduardo; Sarmento, Isabela Valim; da Costa, Charles Santos; Niño, Oscar Mauricio Santamaria; Faria, Rodrigo Alves; Freitas Lima, Leandro Ceotto; Graceli, Jones Bernardes

    2018-01-01

    Organotin (OTs) compounds are organometallic compounds that are widely used in industry, such as in the manufacture of plastics, pesticides, paints, and others. OTs are released into the environment by anthropogenic actions, leading to contact with aquatic and terrestrial organisms that occur in animal feeding. Although OTs are degraded environmentally, reports have shown the effects of this contamination over the years because it can affect organisms of different trophic levels. OTs act as endocrine-disrupting chemicals (EDCs), which can lead to several abnormalities in organisms. In male animals, OTs decrease the weights of the testis and epididymis and reduce the spermatid count, among other dysfunctions. In female animals, OTs alter the weights of the ovaries and uteri and induce damage to the ovaries. In addition, OTs prevent fetal implantation and reduce mammalian pregnancy rates. OTs cross the placental barrier and accumulate in the placental and fetal tissues. Exposure to OTs in utero leads to the accumulation of lipid droplets in the Sertoli cells and gonocytes of male offspring in addition to inducing early puberty in females. In both genders, this damage is associated with the imbalance of sex hormones and the modulation of the hypothalamic–pituitary–gonadal axis. Here, we report that OTs act as reproductive disruptors in vertebrate studies; among the compounds are tetrabutyltin, tributyltin chloride, tributyltin acetate, triphenyltin chloride, triphenyltin hydroxide, dibutyltin chloride, dibutyltin dichloride, diphenyltin dichloride, monobutyltin, and azocyclotin. PMID:29545775

  9. Molecular Characterization of Plasmid-Mediated Oxytetracycline Resistance in Aeromonas salmonicida

    PubMed Central

    Adams, C. A.; Austin, B.; Meaden, P. G.; McIntosh, D.

    1998-01-01

    Using broth conjugation, we found that 19 of 29 (66%) oxytetracycline (OT)-resistant isolates of Aeromonas salmonicida transferred the OT resistance phenotype to Escherichia coli. The OT resistance phenotype was encoded by high-molecular-weight R-plasmids that were capable of transferring OT resistance to both environmental and clinical isolates of Aeromonas spp. The molecular basis for antibiotic resistance in OT-resistant isolates of A. salmonicida was determined. The OT resistance determinant from one plasmid (pASOT) of A. salmonicida was cloned and used in Southern blotting and hybridization experiments as a probe. The determinant was identified on a 5.4-kb EcoRI fragment on R-plasmids from the 19 OT-resistant isolates of A. salmonicida. Hybridization with plasmids encoding the five classes (classes A to E) of OT resistance determinants demonstrated that the OT resistance plasmids of the 19 A. salmonicida isolates carried the class A resistance determinant. Analysis of data generated from restriction enzyme digests showed that the OT resistance plasmids were not identical; three profiles were characterized, two of which showed a high degree of homology. PMID:9797265

  10. Oxytocin response to youth–mother interactions in clinically anxious youth is associated with separation anxiety and dyadic behavior

    PubMed Central

    Lebowitz, Eli R.; Silverman, Wendy K.; Martino, Alyssa M.; Zagoory-Sharon, Orna; Feldman, Ruth; Leckman, James F.

    2017-01-01

    Background Anxiety disorders are common in youth and cause significant distress and impairment to the individual and family. Oxytocin (OT), a nine amino acid peptide, is implicated in anxiety regulation and modulation of close interpersonal and attachment behavior. Anxiety disorders have been linked to low levels of salivary OT in youth. Research has also linked oxytocinergic functioning to social support, warm contact, and bonding, and indicated that contact with attachment figures stimulates OT response. We examined OT response to a brief, positive youth–mother interaction in clinically anxious youth. We investigated whether quality of the youth–mother interaction as well as the presence of particular anxiety disorders, are associated with youth OT response. Method Salivary OT from 41 youth with primary DSM-5 anxiety disorders was assayed before and after a 7-min youth–mother interaction that was later systematically coded by two reliable coders. Youth and mothers also completed rating scales of youth anxiety symptoms. Results Affective touch, maternal sensitivity, maternal intrusiveness, youth engagement, and youth initiative all contributed significantly to predicting youth OT response. Repeated measures analyses showed that when affective touch was high youth had greater OT response. OT response was positively associated with the presence of separation anxiety disorder (SAD) and with child ratings of separation anxiety. Conclusions The findings highlight the importance of maternal and dyadic behavior patterns to oxytocinergic response in clinically anxious youth, shed light on the association between OT and SAD, and point to possible intervention strategies. PMID:28052452

  11. Oxytocin, brain physiology, and functional connectivity: a review of intranasal oxytocin fMRI studies.

    PubMed

    Bethlehem, Richard A I; van Honk, Jack; Auyeung, Bonnie; Baron-Cohen, Simon

    2013-07-01

    In recent years the neuropeptide oxytocin (OT) has become one of the most studied peptides of the human neuroendocrine system. Research has shown widespread behavioural effects and numerous potential therapeutic benefits. However, little is known about how OT triggers these effects in the brain. Here, we discuss some of the physiological properties of OT in the human brain including the long half-life of neuropeptides, the diffuse projections of OT throughout the brain and interactions with other systems such as the dopaminergic system. These properties indicate that OT acts without clear spatial and temporal specificity. Therefore, it is likely to have widespread effects on the brain's intrinsic functioning. Additionally, we review studies that have used functional magnetic resonance imaging (fMRI) concurrently with OT administration. These studies reveal a specific set of 'social' brain regions that are likely to be the strongest targets for OT's potential to influence human behaviour. On the basis of the fMRI literature and the physiological properties of the neuropeptide, we argue that OT has the potential to not only modulate activity in a set of specific brain regions, but also the functional connectivity between these regions. In light of the increasing knowledge of the behavioural effects of OT in humans, studies of the effects of OT administration on brain function can contribute to our understanding of the neural networks in the social brain. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. Indication of a species in an extinction vortex: The ocellated turkey on the Yucatan peninsula, Mexico

    NASA Astrophysics Data System (ADS)

    Kampichler, Christian; Calmé, Sophie; Weissenberger, Holger; Arriaga-Weiss, Stefan Louis

    2010-11-01

    The ocellated turkey Meleagris ocellata (OT) is a large, unmistakable endemic bird of the Yucatan peninsula. The species has suffered a considerable loss of distributional area as well as local abundance between 1980 and 2000 and is classified as endangered according to Mexican norms. We applied Classification Trees and Random Forests in order to determine the factors that most closely explain the observed patterns of distribution and abundance loss, and to develop hypotheses that may guide measures for the protection of the OT. Among the most important predictors of change were variables corresponding to aspects of forest cover and variables on human population and small settlements. OT abundance in 1980, however, was by far the most important predictor for OT abundance change. This is an indication that the OT dynamics are governed by internal rather than by external factors. Medium and low abundances in 1980 inevitably led to a further decrease during the following years, which gives rise to the conclusion that the OT might find itself in an extinction vortex. We suggest the following hypothetical scenario for OT decline: migrant people from other Mexican states colonise forested regions in Yucatan; they establish small settlements; bushmeat hunting is important for their survival; the naïve OT is easy prey; hunting—together with beginning deforestation—reaches a certain level, and local OT abundance falls below a critical threshold; OT continues declining regardless of current social and environmental changes except where there is total protection of both the species and its habitat.

  13. Salivary oxytocin in adolescents with conduct problems and callous-unemotional traits.

    PubMed

    Levy, Tomer; Bloch, Yuval; Bar-Maisels, Meytal; Gat-Yablonski, Galia; Djalovski, Amir; Borodkin, Katy; Apter, Alan

    2015-12-01

    Callous-unemotional (CU) traits correlate with the severity and prognosis of conduct disorder in youth. The neuropeptide oxytocin (OT) has been linked to prosocial behaviors, including empathy and collaboration with others. This study discusses a possible role for OT in the biology of delinquent behavior. We hypothesized that in delinquent youth OT secretion will correlate with the severity of conduct problems and specifically with the level of CU traits. The study group included 67 male adolescents (mean age 16.2 years) undergoing residential treatment, previously assessed by an open clinical interview and history for the psychiatric diagnosis. Staff based Inventory of Callous-Unemotional traits for psychopathy and Strength and Difficulties Questionnaire were administered, and patients' medical and social personal files were systematically coded for previous history of antisocial acts using the Brown-Goodwin Questionnaire. Salivary OT was assayed by ELISA. Salivary OT levels were inversely correlated with conduct problems severity on Strength and Difficulties Questionnaire (r = -0.27; p ≤ 0.01). Recorded history of antisocial acts did not correlate with current OT levels. Odds ratio (OR) for significant CU traits among subjects with conduct problems was increased in low-OT (OR = 14, p ≤ 0.05) but not in high-OT subjects (OR = 6, p ≥ 0.05). Children with conduct problems and low levels of salivary OT are at risk for significant CU traits. These results suggest a possible role for salivary OT as a biomarker for CU traits and conduct problems severity.

  14. Psychosocial needs of older cancer patients: a pilot study abstract.

    PubMed

    Houldin, A D; Wasserbauer, N

    1996-08-01

    Approximately 50% of all cancers occur in persons aged 65 and over (American Cancer Society, 1994). However, the special psychological problems of these patients have been inadequately addressed (Massie & Holland, 1989). The psychosocial needs of older cancer patients were surveyed in this pilot study. Two-thirds of the older adults surveyed experienced concerns or problems. Almost 50% of the sample did not receive adequate assistance in dealing with their emotional needs and 69% did not receive sufficient spiritual support. Older cancer patients experience psychosocial distress and may benefit from professional assistance in dealing with these concerns.

  15. Theoretical Study of Hydrogen Adsorption on the GaN(0001) Surface

    DTIC Science & Technology

    2004-06-01

    423 (1999) 70. [39] J.E. Northrup, J. Neugebauer, R.M. Feenstra, A.R. Smith, Phys. Rev. B 61 (2000) 9932. [40] K. Nakamura, T. Hayashi , A. Tachibana, K...Hasegawa, I.S.T. Tsong, T. Sakurai, T. Ohno , Phys. Rev. Lett. 82 (1999) 3074. [45] K. Raghavachari, Q. Fu, G. Chen, L. Li, C.H. Li, D.C. Law, R.F. Hicks, J...Xue, S. Kuwano, J.T. Sadowski, K.F. Kelly, T. Sakurai, T. Ohno , Phys. Rev. Lett. 84 (2000) 4015. [78] S. Vézian, F. Semond, J. Massies, D.W. Bullock, Z

  16. Terahertz Atmospheric Attenuation and Continuum Effects

    DTIC Science & Technology

    2013-05-01

    comparison of the two pressure-broadened line shapes as well as a Doppler -broadened line shape. As can be seen in the figure, the effect of foreign gas...Conference  April  29-­‐May  3,  2013,  Baltimore,  MD   Figure 2. A Doppler -broadened absorption line with the partial pressure of... Goldman , A., Jacquemart, D., Kleiner, I., Lacome, N., Lafferty, W. J., Mandin, J. Y., Massie, S. T., Mikhailenko, S. N., Miller, C. E., Moazzen

  17. Assessment and pharmacotherapy of depression.

    PubMed

    Van Fleet, Sharon

    2006-04-01

    Depressive disorders are experienced by a significant number of patients with cancer, with reported rates of 0%-58% (Massie, 2004). Numerous studies have demonstrated that depression in patients with cancer is underdiagnosed and inadequately treated as a result of a number of factors (Schwartz, Lander, & Chochinov, 2002). Although more severe or complex situations involving depression or other mental illness necessitate specialist referral, sometimes professionals treating patients with cancer may find the need to initiate treatment. Thus, clinicians need to be aware of basic principles related to the assessment and treatment of depression.

  18. Oxytocin- and arginine vasopressin-containing fibers in the cortex of humans, chimpanzees, and rhesus macaques.

    PubMed

    Rogers, Christina N; Ross, Amy P; Sahu, Shweta P; Siegel, Ethan R; Dooyema, Jeromy M; Cree, Mary Ann; Stopa, Edward G; Young, Larry J; Rilling, James K; Albers, H Elliott; Preuss, Todd M

    2018-05-24

    Oxytocin (OT) and arginine-vasopressin (AVP) are involved in the regulation of complex social behaviors across a wide range of taxa. Despite this, little is known about the neuroanatomy of the OT and AVP systems in most non-human primates, and less in humans. The effects of OT and AVP on social behavior, including aggression, mating, and parental behavior, may be mediated primarily by the extensive connections of OT- and AVP-producing neurons located in the hypothalamus with the basal forebrain and amygdala, as well as with the hypothalamus itself. However, OT and AVP also influence social cognition, including effects on social recognition, cooperation, communication, and in-group altruism, which suggests connectivity with cortical structures. While OT and AVP V1a receptors have been demonstrated in the cortex of rodents and primates, and intranasal administration of OT and AVP has been shown to modulate cortical activity, there is to date little evidence that OT-and AVP-containing neurons project into the cortex. Here, we demonstrate the existence of OT- and AVP-containing fibers in cortical regions relevant to social cognition using immunohistochemistry in humans, chimpanzees, and rhesus macaques. OT-immunoreactive fibers were found in the straight gyrus of the orbitofrontal cortex as well as the anterior cingulate gyrus in human and chimpanzee brains, while no OT-immunoreactive fibers were found in macaque cortex. AVP-immunoreactive fibers were observed in the anterior cingulate gyrus in all species, as well as in the insular cortex in humans, and in a more restricted distribution in chimpanzees. This is the first report of OT and AVP fibers in the cortex in human and non-human primates. Our findings provide a potential mechanism by which OT and AVP might exert effects on brain regions far from their production site in the hypothalamus, as well as potential species differences in the behavioral functions of these target regions. © 2018 Wiley Periodicals, Inc.

  19. Conditioned same-sex partner preference in male rats is facilitated by oxytocin and dopamine: effect on sexually dimorphic brain nuclei.

    PubMed

    Triana-Del Rio, Rodrigo; Tecamachaltzi-Silvarán, Miriam B; Díaz-Estrada, Victor X; Herrera-Covarrubias, Deissy; Corona-Morales, Aleph A; Pfaus, James G; Coria-Avila, Genaro A

    2015-04-15

    Conditioned same-sex partner preference can develop in male rats that undergo cohabitation under the effects of quinpirole (QNP, D2 agonist). Herein, we assessed the development of conditioned same-sex social/sexual preference in males that received either nothing, saline, QNP, oxytocin (OT), or QNP+OT during cohabitation with another male (+) or single-caged (-). This resulted in the following groups: (1) Intact-, (2) Saline+, (3) QNP-, (4) OT-, (5) QNP+, (6) OT+ and (7) QNP/OT+. Cohabitation occurred during 24h in a clean cage with a male partner that bore almond scent on the back as conditioned stimulus. This was repeated every 4 days for a total of three trials. Social and sexual preference were assessed four days after the last conditioning trial in a drug-free test in which experimental males chose between the scented familiar male and a novel sexually receptive female. Results showed that males from groups Intact-, Saline+, QNP- and OT- displayed a clear preference for the female (opposite-sex), whereas groups QNP+, OT+ and QNP/OT+ displayed socio/sexual preference for the male partner (same-sex). In Experiment 2, the brains were processed for Nissl dye and the area size of two sexually dimorphic nuclei (SDN-POA and SON) was compared between groups. Males from groups OT-, OT+ and QNP/OT+ expressed a smaller SDN-POA and groups QNP+ and QNP/OT+ expressed a larger SON. Accordingly, conditioned same-sex social/sexual partner preference can develop during cohabitation under enhanced D2 or OT activity but such preference does not depend on the area size of those sexually dimorphic nuclei. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. [Therapeutic effects of oxytocin in autism: Current status of the research].

    PubMed

    Gauthier, C; Doyen, C; Amado, I; Lôo, H; Gaillard, R

    2016-02-01

    The neuropeptide oxytocin (OT) is an evolutionary highly conserved molecule that plays a part in the regulation of complex social cognition and behaviours. From a pathophysiological point of view, several studies have evidenced dysfunctions of the oxytocinergic system in autism spectrum disorders (ASD): a lowering of plasma OT and genetic or epigenetic anomalies of the OT receptor. Therefore, some authors have hypothesized that an abnormality in the OT neurotransmission may account for several features of autism and that a treatment restoring a normal OT pathway functioning could improve social abilities. OT administration has thus been used in clinical trials, especially in groups of subjects suffering from autism. Some studies found that OT decreased repetitive behaviours, enhanced emotional understanding of speech intonation, improved performance of the Reading the Mind in the Eyes Test and reinforced cooperation. Nevertheless, the findings of the OT administration studies on clinical samples show great diversity. The context, the personality and childhood experiences of the subject could be moderators influencing the effect of exogenous OT. Besides, three mechanisms could play a part in the action of OT on ASD social symptoms: anxiety reduction (with a lowering in the hypothalamic-pituitary-adrenal axis responsiveness and in the amygdale reactivity to social stimuli), increased affiliative motivation (involving the dopaminergic pathway and several regions of the social brain) and enhanced perceptual selectivity and social stimuli salience. To conclude, OT could be a promising molecule used as a treatment to promote social behaviours, helping individuals with ASD to develop new relationships. OT could be administered during a cognitive-behavioural therapy to reinforce the efficacy of such procedures. More studies are needed, on larger samples, to investigate the safety and efficacy of OT administration and to specify optimal dosages and characteristics of

  1. Comprehensive, blinded assessment of balance in orthostatic tremor.

    PubMed

    Bhatti, Danish; Thompson, Rebecca; Xia, Yiwen; Hellman, Amy; Schmaderer, Lorene; Suing, Katie; McKune, Jennifer; Penke, Cynthia; Iske, Regan; Roeder, Bobbi Jo; Siu, Ka-Chun; Bertoni, John M; Torres-Russotto, Diego

    2018-02-01

    Orthostatic Tremor (OT) is a movement disorder characterized by a sensation of unsteadiness and tremors in the 13-18 Hz range present upon standing. The pathophysiology of OT is not well understood but there is a relationship between the sensation of instability and leg tremors. Despite the sensation of unsteadiness, OT patients do not fall often and balance in OT has not been formally assessed. We present a prospective blinded study comparing balance assessment in patients with OT versus healthy controls. We prospectively enrolled 34 surface Electromyography (EMG)-confirmed primary OT subjects and 21 healthy controls. Participants underwent evaluations of balance by blinded physical therapists (PT) with standardized, validated, commonly used balance scales and tasks. OT subjects were mostly female (30/34, 88%) and controls were majority males (13/20, 65%). The average age of OT subjects was 68.5 years (range 54-87) and for controls was 69.4 (range 32-86). The average duration of OT symptoms was 18 years. OT subjects did significantly worse on all the balance scales and on most balance tasks including Berg Balance Scale, Functional Gait Assessment, Dynamic Gait Index, Unipedal Stance Test, Functional Reach Test and pull test. Gait speed and five times sit to stand were normal in OT. Common validated balance scales are significantly abnormal in primary OT. Despite the objective finding of impaired balance, OT patients do not commonly have falls. The reported sensation of unsteadiness in this patient population seems to be out of proportion to the number of actual falls. Further studies are needed to determine which components of commonly used balance scales are affected by a sensation of unsteadiness and fear of falling. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Overtraining Syndrome

    PubMed Central

    Kreher, Jeffrey B.; Schwartz, Jennifer B.

    2012-01-01

    Context: Fatigue and underperformance are common in athletes. Understanding overtraining syndrome (OTS) is helpful in the evaluation, management, and education of athletes. Evidence Acquisition: Relevant articles in English were searched with OVID (1948-2011) and PubMed using the following keywords: overtraining syndrome, overtraining, overreaching, unexplained underperformance, staleness, pathophysiology, management, treatment, evaluation. Bibliographies were reviewed for additional resources. Results: OTS appears to be a maladapted response to excessive exercise without adequate rest, resulting in perturbations of multiple body systems (neurologic, endocrinologic, immunologic) coupled with mood changes. Many hypotheses of OTS pathogenesis are reviewed, and a clinical approach to athletes with possible OTS (including history, testing, and prevention) is presented. Conclusions: OTS remains a clinical diagnosis with arbitrary definitions per the European College of Sports Science’s position statement. History and, in most situations, limited serologies are helpful. However, much remains to be learned given that most past research has been on athletes with overreaching rather than OTS. PMID:23016079

  3. AFM investigation of effect of absorbed water layer structure on growth mechanism of octadecyltrichlorosilane self-assembled monolayer on oxidized silicon

    SciTech Connect

    Li, Shaowei; Zheng, Yanjun, E-mail: zhengyj@cup.edu.cn; Chen, Changfeng

    2016-06-28

    The growth mechanism of an octadecyltrichlorosilane (OTS) self-assembled monolayer on a silicon oxide surface at various relative humidities has been investigated. Atomic force microscopy images show that excess water may actually hinder the nucleation and growth of OTS islands. A moderate amount of water is favorable for the nucleation and growth of OTS islands in the initial stage; however, the completion of the monolayer is very slow in the final stage. The growth of OTS islands on a low-water-content surface maintains a relatively constant speed and requires the least amount of time. The mobility of water molecules is thought tomore » play an important role in the OTS monolayers, and a low-mobility water layer provides a steady condition for OTS monolayer growth.« less

  4. Energy conservation in stressed rats exposed to an oxytocin-injected cage mate.

    PubMed

    Agren, Greta; Lundeberg, Thomas

    2002-08-07

    In previous studies we found indications of stress reduction in saline-injected rats when exposed to an oxytocin (OT)-injected cage mate. Olfactory impairment and OT antagonist treatment abolished the effects. This suggested an olfactorily mediated oxytocinergic stress-inhibitory mechanism. To test this hypothesis bodyweight, tail skin temperatures, food-intake and plasma ACTH and corticosterone concentrations were analysed. Suppressed weight loss and decreased stress-hormone release was found in saline-injected rats exposed to an OT-injected cage mate, but not in OT antagonist-injected rats, supporting the hypothesis. Our results suggest that OT in a stressed animal can inhibit the olfactory stress cues emitted, and that the olfactory cues from the stressed animal can influence an OT in pathway in the odour recipient animals to reduce stress effects.

  5. Oxytocin and social cognition in rhesus macaques: Implications for understanding and treating human psychopathology

    PubMed Central

    Chang, Steve W. C.; Platt, Michael L.

    2013-01-01

    Converging evidence from humans and non-human animals indicates that the neurohypophysial hormone oxytocin (OT) evolved to serve a specialized function in social behavior in mammals. Although OT-based therapies are currently being evaluated as remedies for social deficits in neuropsychiatric disorders, precisely how OT regulates complex social processes remains largely unknown. Here we describe how a non-human primate model can be used to understand the mechanisms by which OT regulates social cognition and thereby inform its clinical application in humans. We focus primarily on recent advances in our understanding of OT-mediated social cognition in rhesus macaques (Macaca mulatta), supplemented by discussion of recent work in humans, other primates, and rodents. Together, these studies endorse the hypothesis that OT promotes social exploration both by amplifying social motivation and by attenuating social vigilance. PMID:24231551

  6. AFM investigation of effect of absorbed water layer structure on growth mechanism of octadecyltrichlorosilane self-assembled monolayer on oxidized silicon

    NASA Astrophysics Data System (ADS)

    Li, Shaowei; Zheng, Yanjun; Chen, Changfeng

    2016-06-01

    The growth mechanism of an octadecyltrichlorosilane (OTS) self-assembled monolayer on a silicon oxide surface at various relative humidities has been investigated. Atomic force microscopy images show that excess water may actually hinder the nucleation and growth of OTS islands. A moderate amount of water is favorable for the nucleation and growth of OTS islands in the initial stage; however, the completion of the monolayer is very slow in the final stage. The growth of OTS islands on a low-water-content surface maintains a relatively constant speed and requires the least amount of time. The mobility of water molecules is thought to play an important role in the OTS monolayers, and a low-mobility water layer provides a steady condition for OTS monolayer growth.

  7. Oxytocin differentially alters resting state functional connectivity between amygdala subregions and emotional control networks: Inverse correlation with depressive traits.

    PubMed

    Eckstein, Monika; Markett, Sebastian; Kendrick, Keith M; Ditzen, Beate; Liu, Fang; Hurlemann, Rene; Becker, Benjamin

    2017-04-01

    The hypothalamic neuropeptide oxytocin (OT) has received increasing attention for its role in modulating social-emotional processes across species. Previous studies on using intranasal-OT in humans point to a crucial engagement of the amygdala in the observed neuromodulatory effects of OT under task and rest conditions. However, the amygdala is not a single homogenous structure, but rather a set of structurally and functionally heterogeneous nuclei that show distinct patterns of connectivity with limbic and frontal emotion-processing regions. To determine potential differential effects of OT on functional connectivity of the amygdala subregions, 79 male participants underwent resting-state fMRI following randomized intranasal-OT or placebo administration. In line with previous studies OT increased the connectivity of the total amygdala with dorso-medial prefrontal regions engaged in emotion regulation. In addition, OT enhanced coupling of the total amygdala with cerebellar regions. Importantly, OT differentially altered the connectivity of amygdala subregions with distinct up-stream cortical nodes, particularly prefrontal/parietal, and cerebellar down-stream regions. OT-induced increased connectivity with cerebellar regions were largely driven by effects on the centromedial and basolateral subregions, whereas increased connectivity with prefrontal regions were largely mediated by right superficial and basolateral subregions. OT decreased connectivity of the centromedial subregions with core hubs of the emotional face processing network in temporal, occipital and parietal regions. Preliminary findings suggest that effects on the superficial amygdala-prefrontal pathway were inversely associated with levels of subclinical depression, possibly indicating that OT modulation may be blunted in the context of increased pathological load. Together, the present findings suggest a subregional-specific modulatory role of OT on amygdala-centered emotion processing networks in

  8. Oxytocin administration in the basolateral and central nuclei of amygdala moderately suppresses food intake.

    PubMed

    Klockars, Oscar A; Klockars, Anica; Levine, Allen S; Olszewski, Pawel K

    2018-04-11

    Oxytocin (OT) at acting central nuclei decreases meal size and reduces intake of palatable sweet solutions. It remains largely unclear as to which brain sites mediate OT's effect on palatability versus energy or the combination of those aspects of consumption. Here, we expanded the search for sites that mediate anorexigenic properties of OT by focusing on two subdivisions of the amygdala, its central (CNA) and basolateral (BLA) nuclei. We injected OT directly into the BLA or CNA in rats and assessed intake of standard chow induced by energy deprivation and intake of sweet solutions in nondeprived animals. We examined whether these effects are reversible by OT receptor (OTr) antagonism and whether OT presence in BLA or CNA induces taste aversion. We also determined the effect of energy deprivation and exposure to sweet saccharin on BLA and CNA expression of OTr mRNA. OT administration in BLA at 0.3 μg and in CNA at 1 μg reduced standard chow intake after deprivation by ~25%. Only administration of OT in BLA was effective in suppressing consumption of sucrose and saccharin solutions. The anorexigenic effects of OT in BLA and CNA were attenuated by OTr antagonist, L-368,899, pretreatment. OT at anorexigenic doses did not promote acquisition of taste aversion. BLA OTr mRNA expression was affected by exposure to palatable saccharin, whereas that of CNA OTr, by energy deprivation. OT in the amygdala moderately decreases food intake. The functional relationship between amygdalar OT and energy intake versus palatability-driven intake depends on the discrete localization of the OTr within this complex structure.

  9. An Early Postnatal Oxytocin Treatment Prevents Social and Learning Deficits in Adult Mice Deficient for Magel2, a Gene Involved in Prader-Willi Syndrome and Autism.

    PubMed

    Meziane, Hamid; Schaller, Fabienne; Bauer, Sylvian; Villard, Claude; Matarazzo, Valery; Riet, Fabrice; Guillon, Gilles; Lafitte, Daniel; Desarmenien, Michel G; Tauber, Maithé; Muscatelli, Françoise

    2015-07-15

    Mutations of MAGEL2 have been reported in patients presenting with autism, and loss of MAGEL2 is also associated with Prader-Willi syndrome, a neurodevelopmental genetic disorder. This study aimed to determine the behavioral phenotype of Magel2-deficient adult mice, to characterize the central oxytocin (OT) system of these mutant mice, and to test the curative effect of a peripheral OT treatment just after birth. We assessed the social and cognitive behavior of Magel2-deficient mice, analyzed the OT system of mutant mice treated or not by a postnatal administration of OT, and determined the effect of this treatment on the brain. Magel2 inactivation induces a deficit in social recognition and social interaction and a reduced learning ability in adult male mice. In these mice, we reveal anatomical and functional modifications of the OT system and show that these defects change from birth to adulthood. Daily administration of OT in the first postnatal week was sufficient to prevent deficits in social behavior and learning abilities in adult mutant male mice. We show that this OT treatment partly restores a normal OT system. Thus, we report that an alteration of the OT system around birth has long-term consequences on behavior and on cognition. Importantly, an acute OT treatment of Magel2-deficient pups has a curative effect. Our study reveals that OT plays a crucial role in setting social behaviors during a period just after birth. An early OT treatment in this critical period could be a novel therapeutic approach for the treatment of neurodevelopmental disorders such as Prader-Willi syndrome and autism. Copyright © 2015 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.

  10. Low-dose oxytocin delivered intranasally with Breath Powered device affects social-cognitive behavior: a randomized four-way crossover trial with nasal cavity dimension assessment

    PubMed Central

    Quintana, D S; Westlye, L T; Rustan, Ø G; Tesli, N; Poppy, C L; Smevik, H; Tesli, M; Røine, M; Mahmoud, R A; Smerud, K T; Djupesland, P G; Andreassen, O A

    2015-01-01

    Despite the promise of intranasal oxytocin (OT) for modulating social behavior, recent work has provided mixed results. This may relate to suboptimal drug deposition achieved with conventional nasal sprays, inter-individual differences in nasal physiology and a poor understanding of how intranasal OT is delivered to the brain in humans. Delivering OT using a novel ‘Breath Powered' nasal device previously shown to enhance deposition in intranasal sites targeted for nose-to-brain transport, we evaluated dose-dependent effects on social cognition, compared response with intravenous (IV) administration of OT, and assessed nasal cavity dimensions using acoustic rhinometry. We adopted a randomized, double-blind, double-dummy, crossover design, with 16 healthy male adults completing four single-dose treatments (intranasal 8 IU (international units) or 24 IU OT, 1 IU OT IV and placebo). The primary outcome was social cognition measured by emotional ratings of facial images. Secondary outcomes included the pharmacokinetics of OT, vasopressin and cortisol in blood and the association between nasal cavity dimensions and emotional ratings. Despite the fact that all the treatments produced similar plasma OT increases compared with placebo, there was a main effect of treatment on anger ratings of emotionally ambiguous faces. Pairwise comparisons revealed decreased ratings after 8 IU OT in comparison to both placebo and 24 IU OT. In addition, there was an inverse relationship between nasal valve dimensions and anger ratings of ambiguous faces after 8-IU OT treatment. These findings provide support for a direct nose-to-brain effect, independent of blood absorption, of low-dose OT delivered from a Breath Powered device. PMID:26171983

  11. Low-dose oxytocin delivered intranasally with Breath Powered device affects social-cognitive behavior: a randomized four-way crossover trial with nasal cavity dimension assessment.

    PubMed

    Quintana, D S; Westlye, L T; Rustan, Ø G; Tesli, N; Poppy, C L; Smevik, H; Tesli, M; Røine, M; Mahmoud, R A; Smerud, K T; Djupesland, P G; Andreassen, O A

    2015-07-14

    Despite the promise of intranasal oxytocin (OT) for modulating social behavior, recent work has provided mixed results. This may relate to suboptimal drug deposition achieved with conventional nasal sprays, inter-individual differences in nasal physiology and a poor understanding of how intranasal OT is delivered to the brain in humans. Delivering OT using a novel 'Breath Powered' nasal device previously shown to enhance deposition in intranasal sites targeted for nose-to-brain transport, we evaluated dose-dependent effects on social cognition, compared response with intravenous (IV) administration of OT, and assessed nasal cavity dimensions using acoustic rhinometry. We adopted a randomized, double-blind, double-dummy, crossover design, with 16 healthy male adults completing four single-dose treatments (intranasal 8 IU (international units) or 24 IU OT, 1 IU OT IV and placebo). The primary outcome was social cognition measured by emotional ratings of facial images. Secondary outcomes included the pharmacokinetics of OT, vasopressin and cortisol in blood and the association between nasal cavity dimensions and emotional ratings. Despite the fact that all the treatments produced similar plasma OT increases compared with placebo, there was a main effect of treatment on anger ratings of emotionally ambiguous faces. Pairwise comparisons revealed decreased ratings after 8 IU OT in comparison to both placebo and 24 IU OT. In addition, there was an inverse relationship between nasal valve dimensions and anger ratings of ambiguous faces after 8-IU OT treatment. These findings provide support for a direct nose-to-brain effect, independent of blood absorption, of low-dose OT delivered from a Breath Powered device.

  12. Evaluations of refraction competencies of ophthalmic technicians in Mozambique.

    PubMed

    Shah, Kajal; Naidoo, Kovin; Chagunda, Margarida; Loughman, James

    2016-01-01

    Ophthalmic technicians (OT) work at health facilities in Mozambique and are trained to provide primary and secondary eye care services including basic refraction. This study was designed to assess OT competence and confidence in refraction, and investigate whether an upskilling programme is effective in developing their competence and confidence at refraction. Thirty-one trainee OTs and 16 qualified OTs were recruited to the study. A background questionnaire was administered to determine the demographic profile of the OTs. A confidence levels questionnaire explored their self-reported skills. Clinical competencies were assessed in relation to knowledge (theory exam) and clinical skills (patient exams). 11 OTs were upskilled and the clinical evaluations carried out post training. Initial evaluations demonstrated that confidence and competence levels varied depending on the OTs training (location and duration), and their location of work (clinical load, availability of equipment and other eye care personnel). The qualified OTs were more competent than trainee OTs in most of the evaluations. Post upskilling results demonstrated significant positive impact on confidence and competence levels. These evaluations identified factors affecting the refraction competencies of the OTs and demonstrated that upskilling is effective in improving confidence and competence levels for refraction. They demonstrate the need for a refraction competency framework. The overarching aim of this research was to inform the development of a nationwide programme of OT mentoring, upskilling and leading to the establishment of clinical competency standards for the new OT curricula, relevant to the professional demands. Copyright © 2014 Spanish General Council of Optometry. Published by Elsevier Espana. All rights reserved.

  13. Manipulation of the oxytocin system alters social behavior and attraction in pair-bonding primates, Callithrix penicillata.

    PubMed

    Smith, Adam S; Agmo, Anders; Birnie, Andrew K; French, Jeffrey A

    2010-02-01

    The establishment and maintenance of stable, long-term male-female relationships, or pair-bonds, are marked by high levels of mutual attraction, selective preference for the partner, and high rates of sociosexual behavior. Central oxytocin (OT) affects social preference and partner-directed social behavior in rodents, but the role of this neuropeptide has yet to be studied in heterosexual primate relationships. The present study evaluated whether the OT system plays a role in the dynamics of social behavior and partner preference during the first 3 weeks of cohabitation in male and female marmosets, Callithrix penicillata. OT activity was stimulated by intranasal administration of OT, and inhibited by oral administration of a non-peptide OT-receptor antagonist (L-368,899; Merck). Social behavior throughout the pairing varied as a function of OT treatment. Compared to controls, marmosets initiated huddling with their social partner more often after OT treatments but reduced proximity and huddling after OT antagonist treatments. OT antagonist treatment also eliminated food sharing between partners. During the 24-h preference test, all marmosets interacted more with an opposite-sex stranger than with the partner. By the third-week preference test, marmosets interacted with the partner and stranger equally with the exception that intranasal-OT treatments facilitated initial partner-seeking behavior over initial contact with the stranger. Our findings demonstrate that pharmacological manipulations of OT activity alter partner-directed social behavior during pair interactions, suggesting that central OT may facilitate the process of pair-bond formation and social relationships in marmoset monkeys. Published by Elsevier Inc.

  14. Therapeutic Evaluation of Mesenchymal Stem Cells in Chronic Gut Inflammation

    DTIC Science & Technology

    2015-09-01

    activate mouse splenocytes obtained from OT2 transgenic (tg) mice with ovalbumin peptide ( OVA ) and quantify T cell proliferation in vitro. The T...cell receptors (TCR) on CD4+ T cells in OT2 tg mice recognize only OVA presented by the major histocompatibility complex II (MHC II) expressed on...mouse OT2 splenocytes with OVA in the presence of increasing numbers of un-manipulated or irradiated hMSCs, we observe little or no suppression of T

  15. Stereospecificity of Antidotes and Their Mechanism of Action in Intoxications with Organophosphorus Anticholinesterases

    DTIC Science & Technology

    1988-02-01

    hyoscyamine prevented oxotremorine (OT)-induced tremor, confirming Its physiological relevance *WICLASSIFIEDRJNUMM1D 03 SAME AS RPT. (3oDTIC USERS Enlas rie...us to believe that the effect is specific. Diazepam prevented oxotremorine (OT)-induced tremor when injected both before and after the OT admini...diazepam and I-hyoscyamine on oxotremorine -induced hypothermia 23 Table 2. Influence of diazepam on specific binding of I-hyoscyamine 24 Table 3

  16. Human Factors Engineering Program for Intercontinental Ballistic Missile Systems, (MIL-STD-1794(USAF))

    DTIC Science & Technology

    1986-10-01

    opeational test and evaluation (OT&R). The OT&B Is comprised of Initial operational test and evaluation ( IOT &R) and follow-on test and evaluation (FOT&R). OT&I...BP HYL FVAC beating, ventilation and air conditioning am. ICBM Intercntinental ballistic missile an. IOT &R Initial operational test and *valuation so...and maintenance vehicles (stop- B pod, engine idle-exterior), facility equipment utility rooms, heating, ventilation and air conditioning ( HVAC

  17. Manipulation of the oxytocin system alters social behavior and attraction in pair-bonding primates, Callithrix penicillata

    PubMed Central

    Smith, Adam S.; Ågmo, Anders; Birnie, Andrew K.; French, Jeffrey A.

    2010-01-01

    The establishment and maintenance of stable, long-term male-female relationships, or pair bonds, are marked by high levels of mutual attraction, selective preference for the partner, and high rates of sociosexual behavior. Central oxytocin (OT) affects social preference and partner-directed social behavior in rodents, but the role of this neuropeptide has yet to be studied in heterosexual primate relationships. The present study evaluated whether the OT system plays a role in the dynamics of social behavior and partner preference during the first three weeks of cohabitation in male and female marmosets, Callithrix penicillata. OT activity was stimulated by intranasal administration of OT, and inhibited by oral administration of a non-peptide OT-receptor antagonist (L-368,899; Merck). Social behavior throughout the pairing varied as a function of OT treatment. Compared to controls, marmosets initiated huddling with their social partner more often after OT treatments but reduced proximity and huddling after OT antagonist treatments. OT antagonist treatment also eliminated food sharing between partners. During the 24-h preference test, all marmosets interacted more with an opposite-sex stranger than with the partner. By the third-week preference test, marmosets interacted with the partner and stranger equally with the exception that intranasal-OT treatments facilitated initial partner-seeking behavior over initial contact with the stranger. Our findings demonstrate that pharmacological manipulations of OT activity alter partner-directed social behavior during pair interactions, suggesting that central OT may facilitate the process of pair-bond formation and social relationships in marmoset monkeys. PMID:20025881

  18. Associations between oxytocin receptor gene (OXTR) methylation, plasma oxytocin, and attachment across adulthood.

    PubMed

    Ebner, Natalie C; Lin, Tian; Muradoglu, Melis; Weir, Devon H; Plasencia, Gabriela M; Lillard, Travis S; Pournajafi-Nazarloo, Hossein; Cohen, Ronald A; Sue Carter, C; Connelly, Jessica J

    2018-02-02

    The neuropeptide oxytocin (OT) has been implicated in a wide range of affiliative processes. OT exerts its functions via OT receptors, which are encoded by the oxytocin receptor gene (OXTR). Epigenetic modification of OXTR through the process of DNA methylation has been associated with individual differences in behavioral phenotypes. Specifically, lower levels of OXTR methylation have been linked to better social and affective functioning. However, research on epigenetic mechanisms of OXTR is scarce in non-clinical populations, and even less is known about epigenetic variability across adulthood. The present study assessed methylation levels at OXTR CpG site -934 and plasma OT levels in 22 young (20-31 years, M = 23.6) and 34 older (63-80 years, M = 71.4) participants. Lower levels of OXTR methylation and higher plasma OT levels were associated with less self-reported attachment anxiety in young but not older participants, with largely independent contributions of OXTR methylation and plasma OT levels. In contrast, in the overall sample, lower levels of OXTR methylation were associated with higher self-reported attachment avoidance. Age analysis suggested that these results were largely driven by young adults. Plasma OT levels were unrelated to attachment avoidance. Taken together, these findings support the emerging notion in the literature that epigenetic properties of OXTR, in addition to endogenous OT levels, are related to adult attachment. Further, the age effects observed in the associations between OXTR methylation, plasma OT, and adult attachment emphasize the importance of adopting a developmental perspective when studying properties of the OT system and their relation to affiliative processes. Findings contribute to growing evidence suggesting that epigenetic modification of genes regulating OT pathways and endogenous OT levels are associated with the way people form and maintain intimate social relationships. Copyright © 2018 Elsevier B

  19. Influence of oxytocin on emotion recognition from body language: A randomized placebo-controlled trial.

    PubMed

    Bernaerts, Sylvie; Berra, Emmely; Wenderoth, Nicole; Alaerts, Kaat

    2016-10-01

    The neuropeptide 'oxytocin' (OT) is known to play a pivotal role in a variety of complex social behaviors by promoting a prosocial attitude and interpersonal bonding. One mechanism by which OT is hypothesized to promote prosocial behavior is by enhancing the processing of socially relevant information from the environment. With the present study, we explored to what extent OT can alter the 'reading' of emotional body language as presented by impoverished biological motion point light displays (PLDs). To do so, a double-blind between-subjects randomized placebo-controlled trial was conducted, assessing performance on a bodily emotion recognition task in healthy adult males before and after a single-dose of intranasal OT (24 IU). Overall, a single-dose of OT administration had a significant effect of medium size on emotion recognition from body language. OT-induced improvements in emotion recognition were not differentially modulated by the emotional valence of the presented stimuli (positive versus negative) and also, the overall tendency to label an observed emotional state as 'happy' (positive) or 'angry' (negative) was not modified by the administration of OT. Albeit moderate, the present findings of OT-induced improvements in bodily emotion recognition from whole-body PLD provide further support for a link between OT and the processing of socio-communicative cues originating from the body of others. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  20. Scope of practice and supervision of orthodontic therapists in the United Kingdom: Part 2: a national cross-sectional survey of orthodontic therapists.

    PubMed

    Ahmed, Farooq; Dugdale, Charlotte; Malik, Ovais; Waring, David

    2018-03-01

    Orthodontic therapists (OTs) are the most recent addition to the orthodontic clinical team. The General Dental Council (GDC) and the British Orthodontic Society have formulated guidance and guidelines relating to their scope of practice and level of supervision, however there has been no contemporary UK-based research investigating practice and supervision of OTs. The aim of this study was to investigate the scope of practice and level of supervision of OTs working in the UK. Ethical approval was received from the University of Manchester Research Ethics Committee. An anonymous postal questionnaire was dispatched using postal details acquired through the British Orthodontic Societies mailing list. Three mailings of the questionnaire were conducted. A 74% response rate was achieved. OTs routinely conducted 16 of the 20 procedures from their scope of practice. Uncommon procedures included fitting headgear (24%), lingual appliances (27%), inserting or removing temporary anchorage devices (20%), and taking facebow record (18%). A total of 62% of OTs took patient consent for treatment. 59% were supervised through a written prescription with no direct supervision. OTs were directly supervised for only a quarter of their clinical practice. Orthodontists viewing frequency for OTs varied significantly, and was found to be the following: every 2-4 visits (36%), every other visit (35%), and every visit (26%). OTs mostly carried out the scope of practice as permitted by the GDC. Procedures uncommon to routine orthodontic practice were also uncommon to Orthodontic therapist clinical practice. OTs work mostly through written prescription with no direct supervision.

  1. The knowledge and attitudes of orthodontic trainees towards orthodontic therapists: a national survey.

    PubMed

    Patel, Sameer; Mack, Gavin

    2017-09-01

    To assess the knowledge and attitudes of orthodontic trainees towards orthodontic therapists (OTs) in the UK. Cross-sectional survey. UK-based orthodontic trainees. An electronic survey was sent to all members of the Training Grades Group of the British Orthodontic Society assessing exposure to OTs and their knowledge regarding current supervision guidelines and scope of practice. Attitudes towards OTs were also explored. Seventy-six responses (response rate 57%) were returned. Nearly 90% of trainees had no formal training regarding OTs. A total of 15.5% were aware of the correct current supervision guidelines and there was large variation in the knowledge of OTs' scope of practice. The majority of trainees were happy to supervise OTs, but only 22.4% felt prepared for this during training. In total, 63% of trainees felt that OTs could impact their own future job prospects. Currently, there is minimal formal training provided to trainees regarding the role of OTs. This is reflected in the lack of knowledge regarding supervision guidelines and scope of practice. Overall, trainees felt OTs were positive for the workforce but were concerned regarding the impact of their own future employment.

  2. Combining oxytocin administration and positive emotion inductions: Examining social perception and analytical performance.

    PubMed

    Human, Lauren J; Thorson, Katherine R; Woolley, Joshua D; Mendes, Wendy Berry

    2017-04-01

    Intranasal administration of the hypothalamic neuropeptide oxytocin (OT) has, in some studies, been associated with positive effects on social perception and cognition. Similarly, positive emotion inductions can improve a range of perceptual and performance-based behaviors. In this exploratory study, we examined how OT administration and positive emotion inductions interact in their associations with social and analytical performance. Participants (N=124) were randomly assigned to receive an intranasal spray of OT (40IU) or placebo and then viewed one of three videos designed to engender one of the following emotion states: social warmth, pride, or an affectively neutral state. Following the emotion induction, participants completed social perception and analytical tasks. There were no significant main effects of OT condition on social perception tasks, failing to replicate prior research, or on analytical performance. Further, OT condition and positive emotion inductions did not interact with each other in their associations with social perception performance. However, OT condition and positive emotion manipulations did significantly interact in their associations with analytical performance. Specifically, combining positive emotion inductions with OT administration was associated with worse analytical performance, with the pride induction no longer benefiting performance and the warmth induction resulting in worse performance. In sum, we found little evidence for main or interactive effects of OT on social perception but preliminary evidence that OT administration may impair analytical performance when paired with positive emotion inductions. Copyright © 2017 Elsevier Inc. All rights reserved.

  3. Oxytocin and the Development of Parenting in Humans

    PubMed Central

    Gordon, Ilanit; Zagoory-Sharon, Orna; Leckman, James F.; Feldman, Ruth

    2014-01-01

    Background The nonapeptide oxytocin (OT) has been repeatedly implicated in processes of parent-infant bonding in animal models; yet, its role in the development of human parenting has received less attention and no research has addressed the involvement of OT in the transition to fatherhood. Methods Using a prospective longitudinal design, 160 cohabitating mothers and fathers and their firstborn infant were visited at home during the first postpartum weeks and again at 6 months postpartum. Mothers’ and fathers’ plasma OT was analyzed at each time point with enzyme-linked immunosorbent assay methodology. Interactions between each parent and the infant were observed in the postpartum and microcoded for parenting behavior. Results Overall, parental OT increased across the study period and there were no differences between maternal and paternal OT at each time point. Oxytocin showed high intraindividual stability across the first 6 months of parenting and the OT levels of husband and wife were interrelated at both assessments. Maternal OT was related to the amount of affectionate parenting behaviors, including “motherese” vocalizations, the expression of positive affect, and affectionate touch, whereas paternal OT correlated with the degree of stimulatory parenting behaviors, including proprioceptive contact, tactile stimulation, and object presentation. Conclusions Results are the first to describe plasma OT levels in new fathers and mothers across the transition to parenthood in relation to maternal and paternal typical parenting behaviors. These data may provide a normative basis for the study of parenting under conditions of high risk. PMID:20359699

  4. Navigating surgical fluorescence cameras using near-infrared optical tracking.

    PubMed

    van Oosterom, Matthias; den Houting, David; van de Velde, Cornelis; van Leeuwen, Fijs

    2018-05-01

    Fluorescence guidance facilitates real-time intraoperative visualization of the tissue of interest. However, due to attenuation, the application of fluorescence guidance is restricted to superficial lesions. To overcome this shortcoming, we have previously applied three-dimensional surgical navigation to position the fluorescence camera in reach of the superficial fluorescent signal. Unfortunately, in open surgery, the near-infrared (NIR) optical tracking system (OTS) used for navigation also induced an interference during NIR fluorescence imaging. In an attempt to support future implementation of navigated fluorescence cameras, different aspects of this interference were characterized and solutions were sought after. Two commercial fluorescence cameras for open surgery were studied in (surgical) phantom and human tissue setups using two different NIR OTSs and one OTS simulating light-emitting diode setup. Following the outcome of these measurements, OTS settings were optimized. Measurements indicated the OTS interference was caused by: (1) spectral overlap between the OTS light and camera, (2) OTS light intensity, (3) OTS duty cycle, (4) OTS frequency, (5) fluorescence camera frequency, and (6) fluorescence camera sensitivity. By optimizing points 2 to 4, navigation of fluorescence cameras during open surgery could be facilitated. Optimization of the OTS and camera compatibility can be used to support navigated fluorescence guidance concepts. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).

  5. Twenty years of oxygenated treatment on Russian supercritical plants

    SciTech Connect

    Shitsman, M.E.

    1995-01-01

    The oxygenated treatment (OT) in Russia was first (1973-74) introduced at the 300 MWe Konakovo Supercritical units (SCU). The advantage of OT over AVT especially in rising the reliability of low-radiation boiler section (LRS) promoted to conversion the working units on OT and putting into operation the new SCUs directly with OT. All Russian supercritical plants, besides two of them, operate currently with OT. The conversion of units on OT doesn`t demand much expenditures. OT is simple and reliable. The OT introduction makes the main difficulty connected with the existance of stable stereotype for ammonia feed and supporting the oxygenmore » concentration in feedwater less than 5 ppb. The staff of two mentioned plants gave proof of devotion to AVT by its wide application in the USA and Japan. Now the units with once through boilers at these countries are intensively conversed on OT. It is possible to hope that this circumstance will dismiss at last the stereotype of oxygen fear.« less

  6. Patient compliance with a health care provider referral for an occupational therapy lymphedema consult.

    PubMed

    Dominick, Sally A; Natarajan, Loki; Pierce, John P; Madanat, Hala; Madlensky, Lisa

    2014-07-01

    Limited information exists on breast cancer patients' compliance to attend outpatient appointments with an occupational therapy (OT) lymphedema specialist. The objectives of this study were (1) to examine patient compliance with a health care provider referral for an OT lymphedema consult and (2) to identify potential barriers to compliance. A retrospective chart review of female breast cancer patients at the UC San Diego Health System was conducted. Electronic medical records were queried for breast cancer patients, who received a health care provider referral for an OT lymphedema consult between June 1, 2010 and December 31, 2011. Descriptive statistics and Fisher's exact chi-square tests were used to examine how specific participant characteristics were associated with attending an OT appointment. A total of 210 female patients received an OT referral from a health care provider related to their breast cancer diagnosis. Forty-three (20.5%) patients did not attend an OT appointment. Non-attenders were more likely to have had fewer lymph nodes removed (P<0.01) when compared to attenders. The two most common barriers to attendance were the presence of health problems and undergoing chemotherapy and/or radiation at the time of the OT referral. While most breast cancer patients attended recommended OT lymphedema consults, a substantial number of women might benefit from further education about OT for lymphedema prevention following breast cancer treatment. Further research to understand barriers to attendance is recommended, particularly among women with only sentinel nodes removed.

  7. Cost of Unsuitability: Assessment of Trade-offs Between the Cost of Operational Unsuitability and Research, Development, Test and Evaluation (RDT&E) Costs

    DTIC Science & Technology

    2008-03-01

    July 2004 and OT- IIG from March–June 2005. Only phase OT- IIG was used as the basis for its suitability evaluation, however, as the test aircraft...received several significant “Block B” hardware and software upgrades in March 2005. At OT- IIG the MV-22 met all of the JORD’s R&M requirements save...mean repair time for aborts. B. MEAN FLIGHT HOURS BETWEEN FAILURES—LOGISTICS The DOT&E report following OT- IIG states that the two key measures of

  8. Oxytocin and Aggression.

    PubMed

    de Jong, Trynke R; Neumann, Inga D

    2017-09-02

    The neuropeptide oxytocin (OT) has a solid reputation as a facilitator of social interactions such as parental and pair bonding, trust, and empathy. The many results supporting a pro-social role of OT have generated the hypothesis that impairments in the endogenous OT system may lead to antisocial behavior, most notably social withdrawal or pathological aggression. If this is indeed the case, administration of exogenous OT could be the "serenic" treatment that psychiatrists have for decades been searching for.In the present review, we list and discuss the evidence for an endogenous "hypo-oxytocinergic state" underlying aggressive and antisocial behavior, derived from both animal and human studies. We furthermore examine the reported effects of synthetic OT administration on aggression in rodents and humans.Although the scientific findings listed in this review support, in broad lines, the link between a down-regulated or impaired OT system activity and increased aggression, the anti-aggressive effects of synthetic OT are less straightforward and require further research. The rather complex picture that emerges adds to the ongoing debate questioning the unidirectional pro-social role of OT, as well as the strength of the effects of intranasal OT administration in humans.

  9. Occurrence and health risk assessment of organotins in waterworks and the source water of the Three Gorges Reservoir Region, China.

    PubMed

    Deng, Ting; Wu, Lei; Gao, Jun-Min; Zhou, Bin; Zhang, Ya-Li; Wu, Wen-Nan; Tang, Zhuo-Heng; Jiang, Wen-Chao; Huang, Wei-Lin

    2018-05-01

    The occurrence and health risks of organotins (OTs) in the waterworks and source water in the Three Gorges Reservoir Region (TGRR), China were assessed in this study. Water samples were collected at four waterworks (A, B, C, and D) in March and July 2012 to analyze butyltins (BTs) and phenyltins (PTs) using a gas chromatography-mass spectrometry (GC-MS) system. Our results showed that both the waterworks and their nearby water sources were polluted by OTs, with PTs being the most dominant species. Monobutyltin (MBT), monophenyltin (MPT), diphenyltin (DPT), and triphenyltin (TPT) were detected in most of the analyzed water samples. The highest concentrations of OTs in influents, effluents, and source water in March were 52.81, 17.93, and 55.32 ng Sn L -1 , respectively. Furthermore, significant seasonal changes in OTs pollution were observed in all samples, showing pollution worse in spring compared with summer. The removal of OTs by the conventional treatment processes was not stable among the waterworks. The removal efficiency of OTs in July reached 100% at plant B, while that at plant C was only 38.8%. The source water and influents shared similar composition profiles, concentrations, and seasonal change of OTs, which indicated that OTs in the waterworks were derived from the source water. A health risk assessment indicated that the presence of OTs in the waterworks would not pose a significant health risk to the population, yet their presence should not be ignored.

  10. Time course of the estradiol-dependent induction of oxytocin receptor binding in the ventromedial hypothalamic nucleus of the rat

    SciTech Connect

    Johnson, A.E.; Ball, G.F.; Coirini, H.

    1989-09-01

    Oxytocin (OT) transmission is involved in the steroid-dependent display of sexual receptivity in rats. One of the biochemical processes stimulated by the ovarian steroid 17 beta-estradiol (E2) that is relevant to reproduction is the induction of OT receptor binding in the ventromedial hypothalamic nucleus (VMN). The purpose of these experiments was to determine if E2-induced changes in OT receptor binding in the VMN occur within a time frame relevant to cyclic changes in ovarian steroid secretion. OT receptor binding was measured in the VMN of ovariectomized rats implanted for 0-96 h with E2-containing Silastic capsules. The rate of decay ofmore » OT receptor binding was measured in another group of animals 6-48 h after capsule removal. Receptors were labeled with the specific OT receptor antagonist ({sup 125}I)d(CH2)5(Tyr(Me)2,Thr4,Tyr-NH2(9))OVT, and binding was measured with quantitative autoradiographic methods. In addition, plasma E2 levels and uterine weights were assessed in animals from each treatment condition. Significant increases in E2-dependent OT receptor binding and uterine weight occurred within 24 h of steroid treatment. After E2 withdrawal, OT receptor binding and uterine weight decreased significantly within 24 h. These results are consistent with the hypothesis that steroid modulation of OT receptor binding is necessary for the induction of sexual receptivity.« less

  11. Microbiological Surveillance of Operation Theatres: Five Year Retrospective Analysis from a Tertiary Care Hospital in North India

    PubMed Central

    Najotra, Dipender Kaur; Malhotra, Aneeta Singh; Slathia, Poonam; Raina, Shivani; Dhar, Ashok

    2017-01-01

    Introduction: Microbiological contamination of air and environment in the operation theaters (OTs) are major risk factor for surgical site and other hospital-associated infections. Objectives: The aim was to identify bacterial colonization of surfaces and equipment and to determine the microbial contamination of air in the OTs of a tertiary care hospital. Materials and Methods: Five years (January 2010–December 2014) retrospective analysis of the data obtained from routine microbiological surveillance of the five OTs of the hospital was done. Surface samples were taken with wet swabs from different sites and equipment. Bacterial species were isolated and identified by conventional methods. Air quality surveillance of OTs was done by settle plate method. Results: A total of 4387 samples were collected from surfaces and articles of various OTs. Out of these only 195 (4.4%), samples showed bacterial growth and yielded 210 isolates. The predominant species isolated was Bacillus with 184 (87.6%) isolates followed by coagulase-negative Staphylococcus 17 (8.1%), Staphylococcus aureus 6 (2.9%), and Enteroccoccus spp. 3 (1.4%). Analysis of the OT air samples showed least colony forming unit (cfu) rate of air (27 cfu/m3) in ophthalmology OT and highest rate of 133 cfu/m3 in general surgery OT. Conclusion: The study shows that OTs of our hospital showed a very low bacterial contamination rate on surface swabbing and a cfu count per m3 of air well within permissible limits. PMID:28904915

  12. Melanocortin Receptor Agonists Facilitate Oxytocin-Dependent Partner Preference Formation in the Prairie Vole.

    PubMed

    Modi, Meera E; Inoue, Kiyoshi; Barrett, Catherine E; Kittelberger, Kara A; Smith, Daniel G; Landgraf, Rainer; Young, Larry J

    2015-07-01

    The central melanocortin (MC) system has been widely studied for its effects on food intake and sexual behavior. However, the MC system, and more specifically the MC4 receptor (MC4R), also interacts with neurochemical systems that regulate socioemotional behaviors, including oxytocin (OT) and dopamine. In monogamous prairie voles, OT and dopamine interact to promote partner preference formation, a laboratory measure of an enduring social bond between mates. Here we investigated the effects of MC receptor activation on partner preference formation in prairie voles, as well as the interaction between the MC and OT systems during this process. Peripheral administration of the brain penetrant MC3/4R receptor peptide agonist, Melanotan II (MTII), and the highly selective, small-molecule MC4R agonist, Pf-446687, enhanced partner preference formation in the prairie vole, but not in the non-monogamous meadow vole. MTII-induced partner preferences were enduring, as they were present 1 week after drug manipulation. The prosocial effects of MCR agonists may be mediated, in part, through modulation of OT, as coadministration of an OT receptor antagonist prevented MTII-induced partner preferences. MTII also selectively activated hypothalamic OT neurons and potentiated central OT release. As OT has been shown to enhance some aspects of social cognition in humans, our data suggest that the MC4R may be a viable therapeutic target for enhancing social function in psychiatric disorders, including autism spectrum disorders and schizophrenia, potentially through activation of the OT system.

  13. Melanocortin Receptor Agonists Facilitate Oxytocin-Dependent Partner Preference Formation in the Prairie Vole

    PubMed Central

    Modi, Meera E; Inoue, Kiyoshi; Barrett, Catherine E; Kittelberger, Kara A; Smith, Daniel G; Landgraf, Rainer; Young, Larry J

    2015-01-01

    The central melanocortin (MC) system has been widely studied for its effects on food intake and sexual behavior. However, the MC system, and more specifically the MC4 receptor (MC4R), also interacts with neurochemical systems that regulate socioemotional behaviors, including oxytocin (OT) and dopamine. In monogamous prairie voles, OT and dopamine interact to promote partner preference formation, a laboratory measure of an enduring social bond between mates. Here we investigated the effects of MC receptor activation on partner preference formation in prairie voles, as well as the interaction between the MC and OT systems during this process. Peripheral administration of the brain penetrant MC3/4R receptor peptide agonist, Melanotan II (MTII), and the highly selective, small-molecule MC4R agonist, Pf-446687, enhanced partner preference formation in the prairie vole, but not in the non-monogamous meadow vole. MTII-induced partner preferences were enduring, as they were present 1 week after drug manipulation. The prosocial effects of MCR agonists may be mediated, in part, through modulation of OT, as coadministration of an OT receptor antagonist prevented MTII-induced partner preferences. MTII also selectively activated hypothalamic OT neurons and potentiated central OT release. As OT has been shown to enhance some aspects of social cognition in humans, our data suggest that the MC4R may be a viable therapeutic target for enhancing social function in psychiatric disorders, including autism spectrum disorders and schizophrenia, potentially through activation of the OT system. PMID:25652247

  14. Statistical and methodological considerations for the interpretation of intranasal oxytocin studies

    PubMed Central

    Walum, Hasse; Waldman, Irwin D.; Young, Larry J.

    2015-01-01

    Over the last decade, oxytocin (OT) has received focus in numerous studies associating intranasal administration of this peptide with various aspects of human social behavior. These studies in humans are inspired by animal research, especially in rodents, showing that central manipulations of the OT system affect behavioral phenotypes related to social cognition, including parental behavior, social bonding and individual recognition. Taken together, these studies in humans appear to provide compelling, but sometimes bewildering evidence for the role of OT in influencing a vast array of complex social cognitive processes in humans. In this paper we investigate to what extent the human intranasal OT literature lends support to the hypothesis that intranasal OT consistently influences a wide spectrum of social behavior in humans. We do this by considering statistical features of studies within this field, including factors like statistical power, pre-study odds and bias. Our conclusion is that intranasal OT studies are generally underpowered and that there is a high probability that most of the published intranasal OT findings do not represent true effects. Thus the remarkable reports that intranasal OT influences a large number of human social behaviors should be viewed with healthy skepticism, and we make recommendations to improve the reliability of human OT studies in the future. PMID:26210057

  15. Adaptive coding of the value of social cues with oxytocin, an fMRI study in autism spectrum disorder.

    PubMed

    Andari, Elissar; Richard, Nathalie; Leboyer, Marion; Sirigu, Angela

    2016-03-01

    The neuropeptide oxytocin (OT) is one of the major targets of research in neuroscience, with respect to social functioning. Oxytocin promotes social skills and improves the quality of face processing in individuals with social dysfunctions such as autism spectrum disorder (ASD). Although one of OT's key functions is to promote social behavior during dynamic social interactions, the neural correlates of this function remain unknown. Here, we combined acute intranasal OT (IN-OT) administration (24 IU) and fMRI with an interactive ball game and a face-matching task in individuals with ASD (N = 20). We found that IN-OT selectively enhanced the brain activity of early visual areas in response to faces as compared to non-social stimuli. OT inhalation modulated the BOLD activity of amygdala and hippocampus in a context-dependent manner. Interestingly, IN-OT intake enhanced the activity of mid-orbitofrontal cortex in response to a fair partner, and insula region in response to an unfair partner. These OT-induced neural responses were accompanied by behavioral improvements in terms of allocating appropriate feelings of trust toward different partners' profiles. Our findings suggest that OT impacts the brain activity of key areas implicated in attention and emotion regulation in an adaptive manner, based on the value of social cues. Copyright © 2016 Elsevier Ltd. All rights reserved.

  16. Current Therapy of Acquired Ocular Toxoplasmosis: A Review.

    PubMed

    Lima, Guilherme Sturzeneker Cerqueira; Saraiva, Patricia Grativol Costa; Saraiva, Fábio Petersen

    2015-11-01

    Caused by the parasite Toxoplasma gondii, ocular toxoplasmosis (OT) is the most common form of posterior infectious uveitis. Combined antiparasitic therapy is the standard treatment for OT, but several other schemes have been proposed. The purpose of the present study was to review the literature on the treatment of OT and provide ophthalmologists with up-to-date information to help reduce OT-related visual morbidity. In conclusion, no ideal treatment scheme was identified; currently prescribed therapeutic schemes yield statistically similar functional outcomes.

  17. The relationship between demand and need for orthodontic treatment in high school students in Bangkok.

    PubMed

    Atisook, Pitraporn; Chuacharoen, Rattiya

    2014-07-01

    Orthodontic service is limited in Thailand and cannot meet the demand of the population. (1) To assess the need for orthodontic treatment (OT) using the Index of Orthodontic Treatment Need (IOTN) to analyze the relationship between demand and need for OT and (2) to compare the demand and need for OT between genders. A cross-sectional study was conducted on 450 students aged 12- to 14-years-old in three government high schools in Bangkok. A constructed questionnaire was used to assess demand for OT Clinical examination was done by two orthodontists to determine the needfor OT using the IOTN RESULTS: Most of the students (74.0%) wished to have OT while only one-third (37.5%) had severe need, and one-third (34.4%) had moderate need for OT as judge by the DHC of the IOTN. The AC of the IOTN indicated that most students (55.8%) had mild or no need for OT Females (79%) demanded OT more than males (66% p-value = 0.033) but the need was similar in both sexes. Most functional factors had strong relationships with the demand for OTexcept lower teeth bite on palate, but none was found to be associated with need for OT All of the aesthetic factors had strong relationships with demand for OT There were significant relationships with needs in five categories, 1) crooked, crowded, or spacing teeth, 2) worried when speaking or smiling, 3) had suggestedfor OT 4) breath smell and halitosis, and 5) wanted to put on braces to be like other people or for fashionable reasons. Most of the students requested OT but females had significantly higher demand for OT than males. Most of the samples needed to have OT The aesthetic factors that had strong relationships with the need for OT were 1) crooked, crowded, or spacing teeth, 2) worried when speaking or smiling, 3) had suggested for T07 4) breath smell and halitosis, and 5) wanted to put on braces to be like otherpeople orfor fashionable reasons.

  18. Thalamic Ventral Intermediate Nucleus Deep Brain Stimulation for Orthostatic Tremor.

    PubMed

    Lehn, Alexander C; O'Gorman, Cullen; Olson, Sarah; Salari, Mehri

    2017-01-01

    Orthostatic tremor (OT) was first described in 1977. It is characterized by rapid tremor of 13-18 Hz and can be recorded in the lower limbs and trunk muscles. OT remains difficult to treat, although some success has been reported with deep brain stimulation (DBS). We report a 68-year-old male with OT who did not improve significantly after bilateral thalamic stimulation. Although some patients were described who improved after DBS surgery, more information is needed about the effect of these treatment modalities on OT, ideally in the form of randomized trial data.

  19. Involvement of the oxytocin system in the bed nucleus of the stria terminalis in the sex-specific regulation of social recognition

    PubMed Central

    Dumais, Kelly M.; Alonso, Andrea G.; Immormino, Marisa A.; Bredewold, Remco; Veenema, Alexa H.

    2015-01-01

    Sex differences in the oxytocin (OT) system in the brain may explain why OT often regulates social behaviors in sex-specific ways. However, a link between sex differences in the OT system and sex-specific regulation of social behavior has not been tested. Here, we determined whether sex differences in the OT receptor (OTR) or in OT release in the posterior bed nucleus of the stria terminalis (pBNST) mediates sex-specific regulation of social recognition in rats. We recently showed that, compared to female rats, male rats have a three-fold higher OTR binding density in the pBNST, a sexually dimorphic area implicated in the regulation of social behaviors. We now demonstrate that OTR antagonist (5 ng/0.5 μl/side) administration into the pBNST impairs social recognition in both sexes, while OT (100 pg/0.5 μl/side) administration into the pBNST prolongs the duration of social recognition in males only. These effects seem specific to social recognition, as neither treatment altered total social investigation time in either sex. Moreover, baseline OT release in the pBNST, as measured with in vivo microdialysis, did not differ between the sexes. However, males showed higher OT release in the pBNST during social recognition compared to females. These findings suggest a sex-specific role of the OT system in the pBNST in the regulation of social recognition. PMID:26630388

  20. A common oxytocin receptor gene (OXTR) polymorphism modulates intranasal oxytocin effects on the neural response to social cooperation in humans.

    PubMed

    Feng, C; Lori, A; Waldman, I D; Binder, E B; Haroon, E; Rilling, J K

    2015-09-01

    Intranasal oxytocin (OT) can modulate social-emotional functioning and related brain activity in humans. Consequently, OT has been discussed as a potential treatment for psychiatric disorders involving social behavioral deficits. However, OT effects are often heterogeneous across individuals. Here we explore individual differences in OT effects on the neural response to social cooperation as a function of the rs53576 polymorphism of the oxytocin receptor gene (OXTR). Previously, we conducted a double-blind, placebo-controlled study in which healthy men and women were randomized to treatment with intranasal OT or placebo. Afterwards, they were imaged with functional magnetic resonance imaging while playing an iterated Prisoner's Dilemma Game with same-sex partners. Within the left ventral caudate nucleus, intranasal OT treatment increased activation to reciprocated cooperation in men, but tended to decrease activation in women. Here, we show that these sex differences in OT effects are specific to individuals with the rs53576 GG genotype, and are not found for other genotypes (rs53576 AA/AG). Thus, OT may increase the reward or salience of positive social interactions for male GG homozygotes, while decreasing those processes for female GG homozygotes. These results suggest that rs53576 genotype is an important variable to consider in future investigations of the clinical efficacy of intranasal OT treatment. © 2015 John Wiley & Sons Ltd and International Behavioural and Neural Genetics Society.

  1. Oxytocin Levels in Community-Collected Saliva Samples Transported by Dry Versus Wet Ice.

    PubMed

    Howland, Lois C; Pickler, Rita H; Sullenbarger, Brent A; Connelly, Cynthia D

    2018-01-01

    Oxytocin (OT), a neuropeptide produced primarily in the hypothalamus, is associated with both critical physiological and psychological processes, particularly stress and feelings of affiliation. Increasingly, researchers are seeking ways to reliably incorporate OT as an outcome biomarker in clinical research. Previously, OT levels were measured in plasma or urine. Recently, researchers have measured this biomarker in saliva, particularly when conducting research in clinical and community settings. In spite of increased interest in the use of salivary OT in clinical research, procedures for handling, transport, and analysis of specimens vary. It is not known if significant OT protein degradation occurs if samples are initially transported on wet ice before being frozen. The aim of this study is to evaluate the effect of transport media (wet vs. dry ice) on OT levels derived from saliva collected from 12 postpartum women residing in the community. Saliva collected from each participant was divided between two microcentrifuge tubes (MIDSCI, Valley Park, MO), one placed on wet ice and one on dry ice for transport from the participant's home to the laboratory freezer. Time from collection to storage freezer was recorded. Laboratory personnel, blinded to method of transport, batch processed the samples. No significant differences in OT levels were found by transport method. Despite large interperson variations in OT levels, there were negligible intraperson variations. Although further research is required to identify factors (including transport time) related to interperson variation, this study supports the use of wet ice as a means of transporting salivary OT specimens in community-based research.

  2. Cerebral blood flow reduction associated with orientation for time in amnesic mild cognitive impairment and Alzheimer disease patients.

    PubMed

    Yamashita, Ken-Ichiro; Taniwaki, Yoshihide; Utsunomiya, Hidetsuna; Taniwaki, Takayuki

    2014-01-01

    Impairment of orientation for time (OT) is a characteristic symptom of Alzheimer disease (AD). However, the brain regions underlying OT remain to be elucidated. Using single photon emission computed tomography (SPECT), we examined the brain regions exhibiting hypoperfusion that were associated with OT. We compared regional cerebral blood flow (rCBF) differences between AD and amnesic mild cognitive impairment (aMCI) or normal subjects using 3-dimensional stereotactic surface projection (3D-SSP) analysis. AD patients were divided into OT good and poor groups according to their mean OT scores, and rCBF then compared between the groups to elucidate OT-specific brain areas. 3D-SSP analysis showed reduced rCBF in the left superior parietal lobule (SPL) and bilateral inferior parietal lobule (IPL) in AD patients. In the poor OT group, 3D-SSP analysis revealed hypoperfusion in the bilateral SPL, IPL, posterior cingulated cortex (PCC), and precuneus. Among these areas, region of interest analysis revealed a significant higher number of hypoperfused pixels in the left PCC in the OT poor AD group. Our SPECT study suggested that hypoperfusion in the left SPL and bilateral IPL was AD specific, and reduced rCBF in the left PCC was specifically associated with OT. Copyright © 2014 by the American Society of Neuroimaging.

  3. Radio detections of southern ultracool dwarfs

    NASA Astrophysics Data System (ADS)

    Lynch, C.; Murphy, T.; Ravi, V.; Hobbs, G.; Lo, K.; Ward, C.

    2016-04-01

    We report the results of a volume-limited survey using the Australia Telescope Compact Array to search for transient and quiescent radio emission from 15 Southern hemisphere ultracool dwarfs. We detect radio emission from 2MASSW J0004348-404405 increasing the number of radio loud ultracool dwarfs to 22. We also observe radio emission from 2MASS J10481463-3956062 and 2MASSI J0339352-352544, two sources with previous radio detections. The radio emission from the three detected sources shows no variability or flare emission. Modelling this quiescent emission we find that it is consistent with optically thin gyrosynchrotron emission from a magnetosphere with an emitting region radius of (1-2)R*, magnetic field inclination 20°-80°, field strength ˜10-200 G, and power-law electron density ˜104-108 cm-3. Additionally, we place upper limits on four ultracool dwarfs with no previous radio observations. This increases the number of ultracool dwarfs studied at radio frequencies to 222. Analysing general trends of the radio emission for this sample of 15 sources, we find that the radio activity increases for later spectral types and more rapidly rotating objects. Furthermore, comparing the ratio of the radio to X-ray luminosities for these sources, we find 2MASS J10481463-3956062 and 2MASSI J0339352-352544 violate the Güdel-Benz relation by more than two orders of magnitude.

  4. Attachment and caregiver-infant interaction: a review of observational-assessment tools.

    PubMed

    Tryphonopoulos, Panagiota D; Letourneau, Nicole; Ditommaso, Enrico

    2014-01-01

    The relationship between maternal-infant interaction and attachment quality to infant developmental outcomes has long been established. As children mature, problems stemming from troubled caregiver-infant relations may result in referral to mental health or child protection services. The accurate and appropriate assessment of attachment is critical for early recognition of problematic relations and for informing suitable treatment modalities. Evaluating the quality of attachment poses a challenge for researchers and clinicians seeking to explore the association between infant development and the quality of early caregiving experiences. Although providing a definitive answer to the question of which of these assessment procedures is the single universal standard for measuring attachment quantity is beyond the scope of this article, readers will be provided with a description and comparison of strengths and limitations of the most commonly used measures of attachment, including the Strange Situation Procedure (M.D.S. Ainsworth, M.C. Blehar, E. Waters, & S. Wall, 1978), Attachment Q-Sort (E. Waters & K.E. Deane, 1985), Toddler Attachment Sort (TAS-45; J. Kirkland, D. Bimler, A. Drawneek, M. McKim, & A. Scholmerich, 2004), CARE-Index (P. Crittenden, 1985), Atypical Maternal Behavior Instrument for Assessment and Classification (AMBIANCE; E. Bronfman, E. Parsons, & K. Lyons-Ruth, 1999), Massie-Campbell Scale of Mother-Infant Attachment Indicators During Stress Scale (Attachment During Stress Scale; H.N. Massie & B.K. Campbell, 1983), and the Risky Situation Procedure (D. Paquette & M. Bigras, 2010). © 2014 Michigan Association for Infant Mental Health.

  5. Effect of oral tolerance in a mouse model of allergic rhinitis.

    PubMed

    Shin, Ji-Hyeon; Kang, Jun Myung; Kim, Sung Won; Cho, Jin-Hee; Park, Yong Jin; Kim, Soo Whan

    2010-03-01

    Induction of oral tolerance (OT) is known to prevent allergic inflammation in models of asthma. This study investigated the preventive effect of OT and airway remodeling in a mouse model of allergic rhinitis (AR). An in vivo study using an animal model. Catholic Research Institutes of Medical Science. Forty six-week-old, female BALB/c mice were divided into four groups: control, AR, low-dose OT, and high-dose OT. To induce OT, mice were fed ovalbumin (OVA) before sensitization with OVA/aluminum hydroxide, 1 mg for six days in the low-dose OT group and a 25 mg single dose in the high-dose OT group. Mice in the AR group were fed phosphate-buffered saline. After sensitization followed by challenges with OVA during six weeks, nasal behaviors, interleukin (IL)-13 and interferon gamma (IFN-gamma) levels in nasal lavage (NAL) fluids, as well as OVA-specific IgE levels in serum, were measured. The degree of goblet cell hyperplasia and thickness of lamina propria were observed in nasal tissues by periodic acid-Schiff and Masson's trichrome stain. A P value < 0.05 was accepted as statistically significant. Both OT groups showed a significant decrease in inflammatory cells, IL-13 and IFN-gamma in NAL fluids, as well as OVA-specific IgE levels in serum compared with the AR group. In addition, the degree of goblet cell hyperplasia and thickness of lamina propria were attenuated in both OT groups compared with the AR group. Further, these alterations did not differ significantly between the two OT groups. These results suggest that OT may effectively reduce allergic inflammation as well as airway remodeling in a mouse model of AR. Copyright 2010 American Academy of Otolaryngology-Head and Neck Surgery Foundation. Published by Mosby, Inc. All rights reserved.

  6. CD8+ T cells induce complete regression of advanced ovarian cancers by an interleukin (IL)-2/IL-15 dependent mechanism.

    PubMed

    Yang, Taimei; Wall, Erika M; Milne, Katy; Theiss, Patty; Watson, Peter; Nelson, Brad H

    2007-12-01

    In vitro studies suggest that ovarian cancer evades immune rejection by fostering an immunosuppressive environment within the peritoneum; however, the functional responses of ovarian cancer-specific T cells have not been directly investigated in vivo. Therefore, we developed a new murine model to enable tracking of tumor-specific CD8(+) T-cell responses to advanced ovarian tumors. The ovarian tumor cell line ID8 was transfected to stably express an epitope-tagged version of HER-2/neu (designated Neu(OT-I/OT-II)). After i.p. injection into C57BL/6 mice, ID8 cells expressing Neu(OT-I/OT-II) gave rise to disseminated serous adenocarcinomas with extensive ascites. CD8(+) T cells expressing a transgenic T-cell receptor specific for the OT-I epitope of Neu(OT-I/OT-II) were adoptively transferred into tumor-bearing mice, and functional responses were monitored. Cytokine signaling requirements were evaluated by comparing the responses of wild-type donor T cells with those with genetic deletion of the interleukin (IL)-2/IL-15 receptor beta subunit (CD122) or the IL-2 receptor alpha subunit (CD25). On adoptive transfer into tumor-bearing hosts, wild-type OT-I T cells underwent a striking proliferative response, reaching peak densities of approximately 40% and approximately 90% of CD8(+) T cells in peripheral blood and ascites, respectively. OT-I cells infiltrated and destroyed tumor tissue, and ascites completely resolved within 10 days. By contrast, CD122(-/-) OT-I cells and CD25(-/-) OT-I cells proliferated in blood but failed to accumulate in ascites or tumor tissue or induce tumor regression. Contrary to expectation, advanced ovarian cancers can support extraordinary CD8(+) T-cell proliferation and antitumor activity through an IL-2/IL-15-dependent mechanism.

  7. The Androgen Metabolite, 5α-Androstane-3β,17β-Diol (3β-Diol), Activates the Oxytocin Promoter Through an Estrogen Receptor-β Pathway

    PubMed Central

    Hiroi, Ryoko; Lacagnina, Anthony F.; Hinds, Laura R.; Carbone, David G.; Uht, Rosalie M.

    2013-01-01

    Testosterone has been shown to suppress the acute stress-induced activation of the hypothalamic-pituitary-adrenal axis; however, the mechanisms underlying this response remain unclear. The hypothalamic-pituitary-adrenal axis is regulated by a neuroendocrine subpopulation of medial parvocellular neurons in the paraventricular nucleus of the hypothalamus (PVN). These neurons are devoid of androgen receptors (ARs). Therefore, a possibility is that the PVN target neurons respond to a metabolite in the testosterone catabolic pathway via an AR-independent mechanism. The dihydrotestosterone metabolite, 5α-androstane-3β,17β-diol (3β-diol), binds and activates estrogen receptor-β (ER-β), the predominant ER in the PVN. In the PVN, ER-β is coexpressed with oxytocin (OT). Therefore, we tested the hypothesis that 3β-diol regulates OT expression through ER-β activation. Treatment of ovariectomized rats with estradiol benzoate or 3β-diol for 4 days increased OT mRNA selectively in the midcaudal, but not rostral PVN compared with vehicle-treated controls. 3β-Diol treatment also increased OT mRNA in the hypothalamic N38 cell line in vitro. The functional interactions between 3β-diol and ER-β with the human OT promoter were examined using an OT promoter-luciferase reporter construct (OT-luc). In a dose-dependent manner, 3β-diol treatment increased OT-luc activity when cells were cotransfected with ER-β, but not ER-α. The 3β-diol–induced OT-luc activity was reduced by deletion of the promoter region containing the composite hormone response element (cHRE). Point mutations of the cHRE also prevented OT-luc activation by 3β-diol. These results indicate that 3β-diol induces OT promoter activity via ER-β–cHRE interactions. PMID:23515287

  8. Long-term oxytocin administration enhances the experience of attachment.

    PubMed

    Bernaerts, Sylvie; Prinsen, Jellina; Berra, Emmely; Bosmans, Guy; Steyaert, Jean; Alaerts, Kaat

    2017-04-01

    The neuropeptide 'oxytocin' (OT) is known to play a pivotal role in a variety of complex social behaviors by promoting a prosocial attitude and interpersonal bonding. Previous studies showed that a single-dose of exogenously administered OT can affect trust and feelings of attachment insecurity. With the present study, we explored the effects of two weeks of daily OT administration on measures of state and trait attachment using a double-blind between-subjects randomized placebo-controlled design. In 40 healthy young adult men state and trait attachment were assessed before and after two weeks of daily intranasal OT (24 IU) or placebo using the State Adult Attachment Scale and the Inventory of Parent and Peer Attachment. Mood, social responsiveness and quality of life were additionally assessed as secondary outcome measures. Reductions in attachment avoidance and increases in reports of attachment toward peers were reported after two weeks of OT treatment. Further, treatment-induced changes were most pronounced for participants with less secure attachment towards their peers. indicating that normal variance at baseline modulated treatment response. OT treatment was additionally associated with changes in mood, indicating decreases in feelings of tension and (tentatively) anger in the OT group, not in the placebo group. Further, at the end of the two-week trial, both treatment groups (OT, placebo) reported to experience an increase in social responsiveness and quality of life, but the effects were only specific to the OT-treatment in terms of reports on 'social motivation'. In summary, the observed improvements on state and trait dimensions of attachment after a multiple-dose treatment with OT provide further evidence in support of a pivotal role of OT in promoting the experience of attachment. Copyright © 2017 Elsevier Ltd. All rights reserved.

  9. Long-term exposure to intranasal oxytocin in a mouse autism model

    PubMed Central

    Bales, K L; Solomon, M; Jacob, S; Crawley, J N; Silverman, J L; Larke, R H; Sahagun, E; Puhger, K R; Pride, M C; Mendoza, S P

    2014-01-01

    Oxytocin (OT) is a neuropeptide involved in mammalian social behavior. It is currently in clinical trials for the treatment of autism spectrum disorder (ASD). Previous studies in healthy rodents (prairie voles and C57BL/6J mice) have shown that there may be detrimental effects of long-term intranasal administration, raising the questions about safety and efficacy. To investigate the effects of OT on the aspects of ASD phenotype, we conducted the first study of chronic intranasal OT in a well-validated mouse model of autism, the BTBR T+ Itpr3tf/J inbred strain (BTBR), which displays low sociability and high repetitive behaviors. BTBR and C57BL/6J (B6) mice (N=94) were administered 0.8  IU/kg of OT intranasally, daily for 30 days, starting on day 21. We ran a well-characterized set of behavioral tasks relevant to diagnostic and associated symptoms of autism, including juvenile reciprocal social interactions, three-chambered social approach, open-field exploratory activity, repetitive self-grooming and fear-conditioned learning and memory, some during and some post treatment. Intranasal OT did not improve autism-relevant behaviors in BTBR, except for female sniffing in the three-chambered social interaction test. Male saline-treated BTBR mice showed increased interest in a novel mouse, both in chamber time and sniffing time, whereas OT-treated male BTBR mice showed a preference for the novel mouse in sniffing time only. No deleterious effects of OT were detected in either B6 or BTBR mice, except possibly for the lack of a preference for the novel mouse's chamber in OT-treated male BTBR mice. These results highlight the complexity inherent in understanding the effects of OT on behavior. Future investigations of chronic intranasal OT should include a wider dose range and early developmental time points in both healthy rodents and ASD models to affirm the efficacy and safety of OT. PMID:25386957

  10. Urban/Rural Differences in Therapy Service Use Among Medicaid Children Aged 0-3 With Developmental Conditions in Colorado.

    PubMed

    McManus, Beth M; Lindrooth, Richard; Richardson, Zachary; Rapport, Mary Jane

    2016-01-01

    To describe urban/rural differences in physical (PT) and occupational therapy (OT) service utilization and spending among a sample of young Medicaid-enrolled children with developmental conditions. We analyzed Colorado Children's Medicaid administrative claims from 2006 to 2008. The sample included children who were younger than 36 months of age, had a select developmental condition, and were continuously eligible for each study year up to their third birthday. The study outcomes were number of PT/OT claims, type of PT/OT service, and Medicaid PT/OT spending. Multivariable analyses examined urban/rural differences in PT/OT utilization and spending, adjusting for child, family, and health service characteristics. The sample included 20,959 children. In adjusted analyses, urban children had 2-fold higher odds (odds ratio 2.18, 95% confidence interval 1.89, 2.51) of receiving PT/OT compared to their rural peers. Median annual per-child Medicaid PT/OT spending was $99 higher ($98.79 [$3.23, $194.35]) for urban children versus rural children. When place of PT/OT service and PT/OT procedures was included, this spending difference was drastically reduced. Even accounting for child, family, and health service characteristics, Medicaid PT/OT spending is lower for rural children compared to their urban peers. The difference in spending is largely attributable to utilization of services that are less specialized than urban peers, thus suggesting disparities in access to appropriate PT/OT services. Copyright © 2016 Academic Pediatric Association. Published by Elsevier Inc. All rights reserved.

  11. Sensitive parenting is associated with plasma oxytocin and polymorphisms in the OXTR and CD38 genes.

    PubMed

    Feldman, Ruth; Zagoory-Sharon, Orna; Weisman, Omri; Schneiderman, Inna; Gordon, Ilanit; Maoz, Rina; Shalev, Idan; Ebstein, Richard P

    2012-08-01

    Research in mammals has demonstrated the involvement of oxytocin (OT) in social bond formation; yet, its role in human bonding remains unclear. Plasma OT has been used as a proxy for central activity and studies indicate its association with human affiliative behaviors. Molecular genetic studies also reveal a role for OT neuropathways in shaping the social brain. However, the links between peripheral OT, genetic markers, and their combined contribution to human parenting are unknown. Participants included 352 individuals: 272 mothers and fathers and their 4- to 6-month-old-infants and 80 nonparents. Plasma OT was assayed from adults who were genotyped for oxytocin receptor (OXTR) and CD38 risk alleles associated with social dysfunctions. CD38 is an ectoenzyme that mediates the release of brain OT. Parent-infant interactions were microcoded for parental touch and gaze synchrony and participants reported on parental care in childhood. OXTR (rs2254298 and rs1042778) and CD38 (rs3796863) risk alleles were each associated with lower plasma OT. Reduced plasma OT and both OXTR and CD38 risk alleles were related to less parental touch. The interaction of high plasma OT and low-risk CD38 alleles predicted longer durations of parent-infant gaze synchrony. Parents reporting greater parental care showed higher plasma OT, low-risk CD38 alleles, and more touch toward their infants. Results indicate that peripheral and genetic markers of the extended OT pathway are interrelated and underpin core behaviors associated with human parenting and social engagement. These findings may have important implications for understanding neuropsychiatric disorders marked by early social dysfunctions. Copyright © 2012 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.

  12. Cuban Ocular Toxoplasmosis Epidemiology Study (COTES): incidence and prevalence of ocular toxoplasmosis in Central Cuba.

    PubMed

    Bustillo, Jorge L; Diaz, Jose D; Pacheco, Idarmes C; Gritz, David C

    2015-03-01

    Serological studies indicate that rates of ocular toxoplasmosis (OT) vary geographically, with higher rates in tropical regions. Little is known about population-based rates of active OT. We aimed to describe the epidemiology of OT in Central Cuba. This large-population, cross-sectional cohort study used a prospective database at a large regional referral centre in Central Cuba. The patient database was searched for all patients who presented with OT during the 12-month study period from 1 April 2011 to 31 March 2012. Inclusion criteria were the clinical diagnosis of OT, characterised by focal retinochoroidal inflammation and a response to therapy as expected. Gender-stratified and age-stratified study population data from the 2012 Cuban Census were used to calculate incidence rates and prevalence ratios. Among 279 identified patients with OT, 158 presented with active OT. Of these, 122 new-onset and 36 prior-onset cases were confirmed. Based on the total population in the Sancti Spiritus province (466,106 persons), the overall incidence of active OT was 26.2 per 100,000 person-years (95% CI 21.7 to 31.3) with an annual prevalence ratio of 33.9 per 100,000 persons (95% CI 28.8 to 39.6). The incidence of active OT was lowest in the oldest age group and highest in patients aged 25-44 years (4.5 and 42.1 per 100,000 person-years, respectively). This first report describing population-based rates of OT in the Cuban population highlights the importance of patient age as a likely risk factor for OT. Disease rates were found to be highest in females and young to middle-aged adults. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

  13. Clinical interpretation of opioid tolerance versus opioid-induced hyperalgesia.

    PubMed

    Chen, Lucy; Sein, Michael; Vo, Trang; Amhmed, Shihab; Zhang, Yi; Hilaire, Kristin St; Houghton, Mary; Mao, Jianren

    2014-01-01

    Opioid analgesics are commonly used to manage moderate to severe pain. However, the long-term use of opioids could lead to opioid tolerance (OT) and opioid-induced hyperalgesia (OIH). Distinguishing OIH from OT would impact the practice of opioid therapy because opioid dose adjustment may differentially influence OT and OIH. Currently, there are no standard criteria of OT versus OIH causing considerable ambiguity in clinical interpretation and management of these conditions. The authors designed a practitioner-based survey consisting of 20 targeted questions. Answering these questions would require responders' actual clinical experiences with opioid therapy. The survey was conducted between 2011 and 2012 through direct mails or e-mails to 1,408 physicians who are currently practicing in the United States. The authors find that certain clinical characteristics (eg, increased pain despite opioid dose escalation) are often used by practitioners to make differential diagnosis of OT and OIH despite some overlap in their clinical presentation. A key difference in clinical outcome is that OT and OIH could be improved and exacerbated by opioid dose escalation, respectively. Our survey results revealed a significant knowledge gap in some responders regarding differential diagnosis and management of OT and OIH. The results also identified several issues, such as opioid dose adjustment and clinical comorbidities related to OT and OIH, which require future patient-based studies.

  14. Chronic Intranasal Oxytocin Causes Long-term Impairments in Partner Preference Formation in Male Prairie Voles

    PubMed Central

    Bales, Karen L.; Perkeybile, Allison M.; Conley, Olivia G.; Lee, Meredith H.; Guoynes, Caleigh D.; Downing, Griffin M.; Yun, Catherine R.; Solomon, Marjorie; Jacob, Suma; Mendoza, Sally P.

    2012-01-01

    Background Oxytocin (OT) is a hormone shown to be involved in social bonding in animal models. Intranasal OT is currently in clinical trials for use in disorders such as autism and schizophrenia. We examined long-term effects of intranasal OT given developmentally in the prairie vole (Microtus ochrogaster), a socially monogamous rodent, often used as an animal model to screen drugs that have therapeutic potential for social disorders. Methods We treated voles with one of three dosages of intranasal OT, or saline, from day 21 (weaning) through day 42 (sexual maturity). We examined both social behavior immediately following administration, as well as long-term changes in social and anxiety behavior after treatment ceased. Group sizes varied from 8 to 15 voles (n = 89 voles total). Results Treatment with OT resulted in acute increases in social behavior in males with familiar partners, as seen in humans. However, long-term developmental treatment with low doses of intranasal OT resulted in a deficit in partner preference behavior (a reduction of contact with a familiar opposite-sex partner, used to index pair-bond formation) by males. Conclusions Long-term developmental treatment with OT may show results different to those predicted by short-term studies, as well as significant sex differences and dosage effects. Further animal study is crucial to determining safe and effective strategies for use of chronic intranasal OT, especially during development. PMID:23079235

  15. 76 FR 6191 - Proposed Agency Information Collection Activities; Comment Request-Thrift Financial Report

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-03

    ...' association concerning the definition of core deposits, which was not an element of the OTS's October 5, 2010.... generally accepted accounting principles (GAAP). The OTS received comments from a bankers' association on... breakdowns by loan category, until the FASB finalizes proposed clarifications to its standards for accounting...

  16. MASTER-Tavrida: FSRQ optical flare

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Lipunov, V.; Kornilov, V.; Gorbovskoy, E.

    2018-05-01

    MASTER-Tavrida auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 11h 36m 27.31s +34d 07m 38.9s on 2018-05-16.87374 UT. The OT unfiltered magnitude is (limit 19.7m).

  17. MASTER: bright PSN

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Lipunov, V.; Kornilov, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Gress, O.; Gabovich, A.; Gorbunov, I.; Senik, V.; Vladimirov, V.; Vlasenko, D.; Kuvshinov, D.

    2018-05-01

    MASTER-Tavrida auto-detection system ( Lipunov et al., "MASTER Global Robotic Net",Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 11h 02m 30.30s +55d 35m 55.5s on 2018-05-25.79944 UT. The OT unfiltered magnitude is 17.3m (limit 19.0m).

  18. MASTER Net: optical transients detection

    NASA Astrophysics Data System (ADS)

    Pogrosheva, T.; Gress, O.; Vladimirov, V.; Lipunov, V.; Rebolo, R.; Serra-Ricart, M.; Podesta, R.; Podesta, F.; Francile, C.; Levato, H.; Saffe, C.; Gorbovskoy, E.; Tiurina, N.; Kornilov, V.; Balanutsa, P.; Kuznetsov, A.; Gorbunov, I.; Zimnukhov, D.; Chazov, V.; Vlasenko, D.; Shumkov, V.; Senik, V.; Kuvshinov, D.; Gabovich, A.; Yurkov, V.

    2018-05-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net",Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 17h 01m 47.31s +05d 45m 40.1s on 2018-05-06.23063 UT. The OT unfiltered magnitude is 17.4m (mlim=18.0m).

  19. Examining Interrater Agreement Analyses of a Pilot Special Education Observation Tool

    ERIC Educational Resources Information Center

    Johnson, Evelyn S.; Semmelroth, Carrie L.

    2012-01-01

    This paper reports the results of interrater agreement analyses on a pilot special education teacher evaluation instrument, the Recognizing Effective Special Education Teachers (RESET) Observation Tool (OT). Using evidence-based instructional practices as the basis for the evaluation, the RESET OT is designed for the spectrum of different…

  20. Advancing Ergonomic Expertise in Occupational Therapists: Using an Online Course to Facilitate Knowledge Acquisition and Application of Ergonomics in Daily Practice

    ERIC Educational Resources Information Center

    Finch, Denise E.

    2012-01-01

    Health promotion, prevention of disease and disability, and enhancing participation in meaningful life activities are areas that occupational therapists (OT) can contribute to at the individual, organizational and population levels (AOTA, 2008; Scaffa, Van Slyke & Brownson, 2008). OTs that are skilled in ergonomic assessment and evidence-based…

  1. Inclusion of Medical-Legal Issues in Entry-Level Occupational and Physical Therapy Curricula.

    ERIC Educational Resources Information Center

    Ekelman, Beth A.; Goodman, Glenn; Dal Bello-Haas, Vanina

    2000-01-01

    Directors of 47 occupational therapy (OT) and 65 physical therapy (PT) accredited entry-level programs responded to a survey by indicating their support for inclusion of medical-legal issues in the curriculum. Only 40% of OT and 52% of PT directors believed lawyers should deliver instruction; most felt their faculty were qualified to teach these…

  2. Rural Practitioners' Involvement in Response to Intervention

    ERIC Educational Resources Information Center

    Cahill, Susan M.; McGuire, Beatriz; Krumdick, Nathaniel D.; Lee, Michelle M.

    2015-01-01

    Objective: This study describes perceived levels of involvement in school-based Response to Intervention (RtI) initiatives as reported by occupational therapy (OT) practitioners from different types of communities (i.e., rural, suburban, and urban). In addition, it identifies differences among practice patterns of rural OT practitioners, compared…

  3. Effects of occupational therapy on hospitalized chronic schizophrenia patients with severe negative symptoms.

    PubMed

    Tatsumi, Eri; Yotsumoto, Kayano; Nakamae, Toshimichi; Hashimoto, Takeshi

    2012-05-24

    The aim of this study was to determine whether occupational therapy (OT) can improve the interpersonal relationships and negative symptoms of hospitalized chronic schizophrenia patients with severe negative symptoms. Subjects were 38 patients with chronic schizophrenia. They were randomly divided into an OT group and a control group. Patients in the OT group participated in cooking activities once a week for 15 weeks, while patients in the control group did not. During this period, both groups had the usual treatment except for the cooking activities. In interviews, the patient was asked to place a chair toward the interviewer (a therapist). The angle and distance from the interviewer were taken as indicators of an ability to have interpersonal relationships. Negative symptoms were evaluated with the Scale for the Assessment of Negative Symptoms (SANS). Patients who received OT were able to sit at the smaller angle and shorter distance from the interviewer than before OT (p=0.015 and p=0.013, respectively). The total SANS score was lower after OT than before OT (p=0.033). In the control group, the distance from the interviewer also decreased during the experimental period (p=0.040) but the seating angle and the SANS scores did not change. The results suggest that OT can help to improve a relationship allowing the patient to face the therapist and that it might improve negative symptoms of schizophrenia.

  4. 12 CFR 563e.27 - Strategic plan.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Strategic plan. 563e.27 Section 563e.27 Banks... for Assessing Performance § 563e.27 Strategic plan. (a) Alternative election. The OTS will assess a... strategic plan if: (1) The savings association has submitted the plan to the OTS as provided for in this...

  5. Orthoptic Treatment of Convergence Insufficiency in Parkinson's Disease: A Case Series.

    PubMed

    Kergoat, Hélène; Law, Caroline; Chriqui, Estefania; Kergoat, Marie-Jeanne; Leclerc, Bernard-Simon; Panisset, Michel; Postuma, Ronald; Irving, Elizabeth L

    2017-01-01

    Introduction: This study reports a case series of orthoptic treatment (OT) for convergence insufficiency (CI) in individuals with Parkinson's disease (PD). Method: We are reporting two cases of individuals with PD who completed OT for CI. Both had a confirmed diagnosis of CI, accompanied by CI-type symptomatology. They each underwent an OT program consisting of three office-based visits and 8 weeks of home-based exercises. Treatment outcome was based on the changes measured pre- versus post-OT on the near point of convergence, positive fusional vergences, and symptomatology score. Results: The two participants successfully completed therapy, gained ability to converge, had fewer symptoms, and were satisfied with the OT-induced changes they felt in their day-to-day lives. Conclusion: This case series show that OT for CI in PD is possible. Further research is required as these results demonstrate that OT has the potential to improve symptomatic CI in these patients. In the meantime, the positive results obtained in these two cases should encourage clinicians to consider OT (a therapy with no/minimal risk) for CI in patients with PD whose quality of life is affected by this binocular dysfunction.

  6. MASTER: bright optical blazar flare with historical gamma-ray counterpart

    NASA Astrophysics Data System (ADS)

    Lipunov, V.; Tiurina, N.; Gorbovskoy, E.; Buckley, D.; Kuznetsov, A.; Balanutsa, P.; Kornilov, V.; Vladimirov, V.; Gorbunov, I.; Vlasenko, D.; Potter, S.; Kotze, M.

    2015-12-01

    MASTER-SAAO auto-detection system( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 349171 ) discovered OT source at (RA, Dec) = 14h 27m 41.38s -33d 05m 31.7s on 2015-12-26.07199 UT. The OT unfiltered magnitude is (limit 17.7m).

  7. MASTER: optical transients without history

    NASA Astrophysics Data System (ADS)

    Balanutsa, P.; Lipunov, V.; Gorbovskoy, E.; Buckley, D.; Tiurina, N.; Kornilov, V.; Kuznetsov, A.; Gorbunov, I.; Vlasenko, D.; Popova, E.; Shumkov, V.; Potter, S.; Kotze, M.; Rebolo, R.; Serra-Ricart, M.; Lodieu, N.; Israelian, G.; Sergienko, Yu.; Gabovich, A.; Yurkov, V.; Tlatov, A.; Senik, V.; Dormidontov, D.; Gress, O.; Budnev, N.; Krushinsky, K. Ivanov V.

    2015-10-01

    MASTER-SAAO auto-detection system ( Lipunov et al., Advances in Astronomy, MASTER Global Robotic Net, 2010 ) discovered OT source at (RA, Dec) = 06h 09m 38.37s -58d 21m 58.2s on 2015-10-05.07265 UT. The OT unfiltered magnitude is 16.4 (limit 20.3m).

  8. Oxytocin under opioid antagonism leads to supralinear enhancement of social attention.

    PubMed

    Dal Monte, Olga; Piva, Matthew; Anderson, Kevin M; Tringides, Marios; Holmes, Avram J; Chang, Steve W C

    2017-05-16

    To provide new preclinical evidence toward improving the efficacy of oxytocin (OT) in treating social dysfunction, we tested the benefit of administering OT under simultaneously induced opioid antagonism during dyadic gaze interactions in monkeys. OT coadministered with a μ-opioid receptor antagonist, naloxone, invoked a supralinear enhancement of prolonged and selective social attention, producing a stronger effect than the summed effects of each administered separately. These effects were consistently observed when averaging over entire sessions, as well as specifically following events of particular social importance, including mutual eye contact and mutual reward receipt. Furthermore, attention to various facial regions was differentially modulated depending on social context. Using the Allen Institute's transcriptional atlas, we further established the colocalization of μ-opioid and κ-opioid receptor genes and OT genes at the OT-releasing sites in the human brain. These data across monkeys and humans support a regulatory relationship between the OT and opioid systems and suggest that administering OT under opioid antagonism may boost the therapeutic efficacy of OT for enhancing social cognition.

  9. 12 CFR 516.80 - What language must I use in my publication?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...? (a) English. You must publish the notice in a newspaper printed in the English language. (b) Other than English. If the OTS determines that the primary language of a significant number of adult residents of the community is a language other than English, the OTS may require that you simultaneously...

  10. Instructional Strategies Used to Improve Students' Comfort and Skill in Addressing the Occupational Therapy Process

    ERIC Educational Resources Information Center

    Knecht-Sabres, Lisa Jean; Egan, Brad E.; Wallingford, Minetta S.; Kovic, Mark

    2015-01-01

    The purpose of this study was to investigate the effectiveness of an intentional blending of instructional strategies in an occupational therapy (OT) entry-level master's course. The OT Adult Practice course uses case-based instructional strategies, clinical skills labs, and standardized patient experiences in a dovetailed approach across three…

  11. The Impact of Acquisition Issues and Training on Test and Evaluation

    DTIC Science & Technology

    2000-01-01

    feedback on the OT results before a milestone review and then delivering the OT report, with surprises, virtually at the review. Test Realism There...of the R3D/100 Chip Set and Simulation Heritage. MLRS WE’RE IN THE ARMY NOW TE 14(1 Video, 15 Mins) Cinematics - LTV Aerospace & Defense

  12. MASTER-OAFA: CV outburst

    NASA Astrophysics Data System (ADS)

    Savinov, D.; Shumkov, V.; Lipunov, V.; Podesta, R.; Levato, H.; Kornilov, V.; Gorbovskoy, E.; Tiurina, N.; Balanutsa, P.; Kuznetsov, A.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Pogrosheva, T.; Zimnukhov, D.; Gorbunov, I.; Krylov, A.; Lopez, C.; Podesta, F.; Saffe, C.; Senik, V.; Gabovich, A.; Kuvshinov, D.

    2018-04-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net", Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 18h 25m 25.98s +08d 10m 46.9s on UT. The OT unfiltered magnitude is (mlim=18.4m).

  13. Master-Oafa Psn in PGC620583

    NASA Astrophysics Data System (ADS)

    Savinov, D.; Shumkov, V.; Lipunov, V.; Podesta, R.; Levato, H.; Kornilov, V.; Gorbovskoy, E.; Tiurina, N.; Kuznetsov, A.; Chazov, V.; Vlasenko, D.; Vladimirov, V.; Gress, O.; Gorbunov, I.; Balanutsa, P.; Pogrosheva, T.; Lopez, C.; Podesta, F.; Saffe, C.

    2018-04-01

    MASTER-OAFA auto-detection system ( Lipunov et al., "MASTER Global Robotic Net",Advances in Astronomy, 2010, 30L ) discovered OT source at (RA, Dec) = 11h 31m 23.65s -37d 36m 07.7s on 2018-04-02.00750 UT. The OT unfiltered magnitude is (mlim=18.0m).

  14. Observational difference between gamma and X-ray properties of optically dark and bright GRBs

    SciTech Connect

    Balazs, L. G.; Horvath, I.; Bagoly, Zs.

    2008-05-22

    Using the discriminant analysis of the multivariate statistical analysis we compared the distribution of the physical quantities of the optically dark and bright GRBs, detected by the BAT and XRT on board of the Swift Satellite. We found that the GRBs having detected optical transients (OT) have systematically higher peak fluxes and lower HI column densities than those without OT.

  15. An Exploration of Teacher Perceptions toward Occupational Therapy and Occupational Therapy Practices: A Scoping Review

    ERIC Educational Resources Information Center

    Truong, Vi; Hodgetts, Sandra

    2017-01-01

    Many occupational therapists work in school-based practice, where collaborative consultation between teachers and therapists is critical for intervention effectiveness. This scoping review explores teacher perceptions toward occupational therapy (OT). Three themes emerged: (1) teachers' confusion over the OT role and scope of practice; (2)…

  16. Association between intermediate uveitis and toxocariasis in the Korean population

    PubMed Central

    Kwon, Jin-woo; Sim, Yoonseob; Jee, Donghyun

    2017-01-01

    Abstract The aim of the study was to investigate the characteristics of ocular toxocariasis (OT) presenting with intermediate uveitis in the Korean population. We studied intermediate uveitis patients using ocular and systemic evaluations and a Toxocara IgG serology test. Of 50 intermediate uveitis patients, 19 were seropositive for Toxocara IgG. Of the 19 OT patients, 4 presented with recurrence within 6 months and were significantly younger than nonrecurrence patients (P = 0.009). Thirteen patients had a history of eating raw cow liver. There were 14 males and 5 females in the OT group, and 11 males and 20 females in the non-OT group (P = 0.009). There was bilateral involvement in 7 out of 19 patients in the OT group, and 20 out of 31 patients in non-OT group (P = 0.033). Intermediate uveitis patients in OT were predominately male and had more unilateral presentation than non-OT patients. PMID:28151857

  17. Association between intermediate uveitis and toxocariasis in the Korean population.

    PubMed

    Kwon, Jin-Woo; Sim, Yoonseob; Jee, Donghyun

    2017-02-01

    The aim of the study was to investigate the characteristics of ocular toxocariasis (OT) presenting with intermediate uveitis in the Korean population.We studied intermediate uveitis patients using ocular and systemic evaluations and a Toxocara IgG serology test.Of 50 intermediate uveitis patients, 19 were seropositive for Toxocara IgG. Of the 19 OT patients, 4 presented with recurrence within 6 months and were significantly younger than nonrecurrence patients (P = 0.009). Thirteen patients had a history of eating raw cow liver.There were 14 males and 5 females in the OT group, and 11 males and 20 females in the non-OT group (P = 0.009). There was bilateral involvement in 7 out of 19 patients in the OT group, and 20 out of 31 patients in non-OT group (P = 0.033).Intermediate uveitis patients in OT were predominately male and had more unilateral presentation than non-OT patients.

  18. Working in the Methodological "Outfield": The Case of Bourdieu and Occupational Therapy

    ERIC Educational Resources Information Center

    Watson, Jo; Grenfell, Michael

    2016-01-01

    The article reports on a study of methodological innovation involving occupational therapy (OT) students in higher education (HE). It is based on an original project which examined the experiences and outcomes of non-traditional entrants to pre-registration OT education. A feature of the original project was the application of the epistemological…

  19. Molecular evolution of the neurohypophysial hormone precursors in mammals: Comparative genomics reveals novel mammalian oxytocin and vasopressin analogues.

    PubMed

    Wallis, Michael

    2012-11-01

    Among vertebrates the neurohypophysial hormones show considerable variation. However, in eutherian mammals they have been considered rather conserved, with arginine vasopressin (AVP) and oxytocin (OT) in all species except pig and some relatives, where lysine vasopressin replaces AVP. The availability of genomic data for a wide range of mammals makes it possible to assess whether these peptides and their precursors may be more variable in Eutheria than previously suspected. A survey of these data confirms that AVP and OT occur in most eutherians, but with exceptions. In a New-World monkey (marmoset, Callithrix jacchus) and in tree shrew (Tupaia belangeri), Pro(8)OT replaces OT, confirming a recent report for these species. In armadillo (Dasypus novemcinctus) Leu(3)OT replaces OT, while in tenrec (Echinops telfairi) Thr(4)AVP replaces AVP. In these two species there is also evidence for additional genes/pseudogenes, encoding much-modified forms of AVP, but in most other eutherian species there is no evidence for additional neurohypophysial hormone genes. Evolutionary analysis shows that sequences of eutherian neurohypophysial hormone precursors are generally strongly conserved, particularly those regions encoding active peptide and neurophysin. The close association between OT and VP genes has led to frequent gene conversion of sequences encoding neurophysins. A monotreme, platypus (Ornithorhynchus anatinus) has genes for OT and AVP, organized tail-to-tail as in eutherians, but in marsupials 3-4 genes are present for neurohypophysial hormones, organized tail-to-head as in lower vertebrates. Copyright © 2012 Elsevier Inc. All rights reserved.

  20. Community-Based Occupational Therapy Services for Children: A Quebec Survey on Service Delivery

    ERIC Educational Resources Information Center

    Cotellesso, Annie; Mazer, Barbara; Majnemer, Annette

    2009-01-01

    Community-based occupational therapy (OT) services are intended to promote social integration and minimize disability. The objective of this study was to describe community-based OT services for children in the province of Quebec, Canada. Specific aims included (a) to determine the proportion of Centres Locaux de Services Communautaires (CLSCs)…

  1. 76 FR 6009 - Shareholder Approval of Executive Compensation and Golden Parachute Compensation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-02

    ...)(1) requires that ``[n]ot less frequently than once every 3 years, a proxy or consent or... Exchange Act, requiring that, ``[n]ot less frequently than once every 6 years, a proxy or consent or... determine whether [the say- on-pay vote] will occur every 1, 2, or 3 years.'' \\20\\ As discussed below, this...

  2. Preparation of open tubular capillary columns by in situ ring-opening polymerization and their applications in cLC-MS/MS analysis of tryptic digest.

    PubMed

    Wang, Hongwei; Yao, Yating; Li, Ya; Ma, Shujuan; Peng, Xiaojun; Ou, Junjie; Ye, Mingliang

    2017-08-01

    An open tubular (OT) column (25 μm i.d.) was prepared by in situ ring-opening polymerization of octaglycidyldimethylsilyl polyhedral oligomeric silsesquioxanes (POSS-epoxy) with 4-aminophenyl disulfide (APDS) in a binary porogenic system of ethanol/H 2 O. It was found that porogenic composition played an important role in the formation of OT stationary phases. The ratio of ethanol/H 2 O at 6/1 (v/v) would lead to the fabrication of hybrid monoliths, while the ratio of ethanol/H 2 O at 13/1 (v/v) would result in the synthesis of OT phases. In addition, the effects of precursor content and reaction duration on the thickness of OT stationary phases were investigated. Either lower precursor content or shorter reaction duration would produce thinner layer of OT column. The repeatability of OT columns was evaluated through relative standard deviation (RSD%) with benzene as the analyte. The run-to-run, column-to-column and batch-to-batch repeatabilities were 1.7%, 4.8% and 5.6%, respectively, exhibiting satisfactory repeatability of the OT column. Then tryptic digest of mouse liver proteins was used to evaluate the performance of the resulting OT columns (25 μm i.d. × 2.5 m in length) by cLC-MS/MS analysis, demonstrating their potential in proteome analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. 75 FR 33501 - Definitions for Regulations Affecting All Savings Associations; Money Market Deposit Accounts

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-14

    ... notice and comment is unnecessary under the Administrative Procedure Act (APA) and is implementing this... for dispensing with the 30-day delay required by the Administrative Procedure Act (APA). OTS... reason, OTS has determined for good cause that public notice and comment is unnecessary under the APA...

  4. Novel molecule targets cytokinesis.

    PubMed

    2015-01-01

    Researchers have identified a new compound, OTS964, that blocks cytokinesis. Injections of the compound eliminated human lung tumors that had been transplanted into mice. Clinical trials of OTS964 may start next year. ©2014 American Association for Cancer Research.

  5. 5 CFR 3101.109 - Additional rules for Office of Thrift Supervision employees.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... guarantor of the loan or extension of credit. (3) Exceptions—(i) Credit cards. A covered OTS employee (or a spouse or minor child of a covered OTS employee) may obtain and hold a credit card account established... for the credit card account that are generally applicable to all applicants for the same type of...

  6. 5 CFR 3101.109 - Additional rules for Office of Thrift Supervision employees.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... guarantor of the loan or extension of credit. (3) Exceptions—(i) Credit cards. A covered OTS employee (or a spouse or minor child of a covered OTS employee) may obtain and hold a credit card account established... for the credit card account that are generally applicable to all applicants for the same type of...

  7. 5 CFR 3101.109 - Additional rules for Office of Thrift Supervision employees.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... guarantor of the loan or extension of credit. (3) Exceptions—(i) Credit cards. A covered OTS employee (or a spouse or minor child of a covered OTS employee) may obtain and hold a credit card account established... for the credit card account that are generally applicable to all applicants for the same type of...

  8. 5 CFR 3101.109 - Additional rules for Office of Thrift Supervision employees.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... guarantor of the loan or extension of credit. (3) Exceptions—(i) Credit cards. A covered OTS employee (or a spouse or minor child of a covered OTS employee) may obtain and hold a credit card account established... for the credit card account that are generally applicable to all applicants for the same type of...

  9. 76 FR 4750 - Survey of Information Sharing Practices With Affiliates

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-26

    ... creditors or users of consumer reports. The OTS will use the Survey responses to prepare a report to Congress on the information sharing practices by financial institutions, creditors, or users of consumer... Numbers: N/A. Description: The OTS is required to submit a report to the Congress with any recommendations...

  10. The Perceptions of Students in the Allied Health Professions towards Stroke Rehabilitation Teams and the SLP's Role

    ERIC Educational Resources Information Center

    Insalaco, Deborah; Ozkurt, Elcin; Santiago, Dign

    2007-01-01

    The purpose of this study was to determine the perceptions and knowledge of final-year speech-language pathology (SLP), physical and occupational therapy (PT, OT) students toward stroke rehabilitation teams and the SLPs' roles on them. The investigators adapted a survey developed by (Felsher & Ross, 1994) and administered it to 35 PT, 35 OT, and…

  11. [Interdisciplinary teamwork in the OR: Identification and measurement of teamwork in the operating room].

    PubMed

    Passauer-Baierl, Stefanie; Baschnegger, Heiko; Bruns, Christiane; Weigl, Matthias

    2014-01-01

    Effective teamwork is one of the essentials in conducting successful and safe surgical procedures in the operating theatre (OT). The present paper aims to provide a selective review of various approaches describing effective interdisciplinary teamwork in the OT. Furthermore, it covers observational methods to assess OT teamwork with particular focus on Germany. Our definition of successful surgical teamwork is based on an already established classification system considering five criteria for effective and safe OT teams: coordination, communication, cooperation, leadership, and team monitoring. Well-defined and reliable measures are necessary to examine the quality of OT teamwork. Those methods should entail the special characteristics of the OT team. They should include all phases of the surgical procedure and incorporate all the professions involved (surgeons, surgical nurses, and anaesthetic staff). We conclude that research into methods for the assessment of OTs in Germany needs to be undertaken as a prerequisite to investigating the relationship between OT teamwork and its effects on patient safety and surgical quality. Copyright © 2013. Published by Elsevier GmbH.

  12. Organotins in Neuronal Damage, Brain Function, and Behavior: A Short Review

    PubMed Central

    Ferraz da Silva, Igor; Freitas-Lima, Leandro Ceotto; Graceli, Jones Bernardes; Rodrigues, Lívia Carla de Melo

    2018-01-01

    The consequences of exposure to environmental contaminants have shown significant effects on brain function and behavior in different experimental models. The endocrine-disrupting chemicals (EDC) present various classes of pollutants with potential neurotoxic actions, such as organotins (OTs). OTs have received special attention due to their toxic effects on the central nervous system, leading to abnormal mammalian neuroendocrine axis function. OTs are organometallic pollutants with a tin atom bound to one or more carbon atoms. OT exposure may occur through the food chain and/or contaminated water, since they have multiple applications in industry and agriculture. In addition, OTs have been used with few legal restrictions in the last decades, despite being highly toxic. In addition to their action as EDC, OTs can also cross the blood–brain barrier and show relevant neurotoxic effects, as observed in several animal model studies specifically involving the development of neurodegenerative processes, neuroinflammation, and oxidative stress. Thus, the aim of this short review is to summarize the toxic effects of the most common OT compounds, such as trimethyltin, tributyltin, triethyltin, and triphenyltin, on the brain with a focus on neuronal damage as a result of oxidative stress and neuroinflammation. We also aim to present evidence for the disruption of behavioral functions, neurotransmitters, and neuroendocrine pathways caused by OTs. PMID:29358929

  13. Reading Without the Left Ventral Occipito-Temporal Cortex

    ERIC Educational Resources Information Center

    Seghier, Mohamed L.; Neufeld, Nicholas H.; Zeidman, Peter; Leff, Alex P.; Mechelli, Andrea; Nagendran, Arjuna; Riddoch, Jane M.; Humphreys, Glyn W.; Price, Cathy J.

    2012-01-01

    The left ventral occipito-temporal cortex (LvOT) is thought to be essential for the rapid parallel letter processing that is required for skilled reading. Here we investigate whether rapid written word identification in skilled readers can be supported by neural pathways that do not involve LvOT. Hypotheses were derived from a stroke patient who…

  14. Somato-axodendritic release of oxytocin into the brain due to calcium amplification is essential for social memory.

    PubMed

    Higashida, Haruhiro

    2016-07-01

    Oxytocin (OT) is released into the brain from the cell soma, axons, and dendrites of neurosecretory cells in the hypothalamus. Locally released OT can activate OT receptors, form inositol-1,4,5-trisphosphate and elevate intracellular free calcium (Ca(2+)) concentrations [(Ca(2+)) i ] in self and neighboring neurons in the hypothalamus, resulting in further OT release: i.e., autocrine or paracrine systems of OT-induced OT release. CD38-dependent cyclic ADP-ribose (cADPR) is also involved in this autoregulation by elevating [Ca(2+)] i via Ca(2+) mobilization through ryanodine receptors on intracellular Ca(2+) pools that are sensitive to both Ca(2+) and cADPR. In addition, it has recently been reported that heat stimulation and hyperthermia enhance [Ca(2+)] i increases by Ca(2+) influx, probably through TRPM2 cation channels, suggesting that cADPR and TRPM2 molecules act as Ca(2+) signal amplifiers. Thus, OT release is not simply due to depolarization-secretion coupling. Both of these molecules play critical roles not only during labor and milk ejection in reproductive females, but also during social behavior in daily life in both genders. This was clearly demonstrated in CD38 knockout mice in that social behavior was impaired by reduction of [Ca(2+)] i elevation and subsequent OT secretion. Evidence for the associations of CD38 with social behavior and psychiatric disorder is discussed, especially in subjects with autism spectrum disorder.

  15. Prenatal stress puzzle, the oxytocin piece: Prenatal stress alters the behaviour and autonomic regulation in piglets, insights from oxytocin

    USDA-ARS?s Scientific Manuscript database

    Developmental changes in response to prenatal stressors (PNS) may represent an adaptive strategy to enhance survival traits in the offspring. Yet, PNS could be maladaptive for captive animals, causing anxiety and abnormal social development. Oxytocin (OT) reduces anxiety, whereas OT deficiencies are...

  16. Prenatally stressed piglets 'shut down' in response to separation, oxytocin modulates some effects

    USDA-ARS?s Scientific Manuscript database

    Prenatal stress (PNS) effects may enhance offspring’s survival traits. Yet, PNS could be maladaptive for captive animals, causing anxiety and abnormal social development. Oxytocin (OT) reduces anxiety while OT deficiency results in social behavior alteration. We hypothesized that PNS piglets would b...

  17. 75 FR 26344 - '34 Disclosures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-11

    ... DEPARTMENT OF THE TREASURY Office of Thrift Supervision '34 Disclosures AGENCY: Office of Thrift Supervision (OTS), Treasury. ACTION: Notice and request for comment. SUMMARY: The proposed information... Supervision, 1700 G Street, NW., Washington, DC 20552. SUPPLEMENTARY INFORMATION: OTS may not conduct or...

  18. Evaluation and Opportunities in Overtraining Approaches

    ERIC Educational Resources Information Center

    Roose, Jolanda; de Vries, Wouter R.; Schmikli, Sandor L.; Backx, Frank J. G.; van Doornen, Lorenz J. P.

    2009-01-01

    Overtraining (OT) as a sports phenomenon can be caused by stressors on various levels (physical, emotional, psychological, and social) and evokes responses on these levels. This study evaluated research and new opportunities in the field of OT by introducing an integrated multidisciplinary approach, based on the single and multistressors approach.…

  19. Attitudes of Occupational Therapy Personnel toward Therapists with Disabilities.

    ERIC Educational Resources Information Center

    Elliott, Deborah L.; And Others

    1992-01-01

    Responses were analyzed for a social distance scale and questionnaire on attitudes of 106 registered occupational therapists (OTs) and 98 certified occupational therapy assistants toward OTs with physical or mental disabilities. Results showed generally favorable attitudes toward coworkers with disabilities, except for those with drug dependencies…

  20. 12 CFR 563.180 - Suspicious Activity Reports and other reports and statements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Suspicious Activity Report on the form prescribed by the OTS. (3) SARs required. A savings association or... to supervisory action. (11) Obtaining SARs. A savings association or service corporation may obtain SARs and the instructions from the appropriate OTS Regional Office listed in § 516.40(a) of this...