An investigation of the effects of pitch-roll (de)coupling on helicopter handling qualities

NASA Technical Reports Server (NTRS)

Blanken, C. L.; Pausder, H. J.; Ockier, C. J.

1995-01-01

An extensive investigation of the effects of pitch-roll coupling on helicopter handling qualities was performed by the U.S. Army and Deutsche Forschungsanstalt fur Luft- und Raumfahrt (DLR), using a NASA ground-based and a DLR in-flight simulator. Over 90 different coupling configurations were evaluated using a high gain roll-axis tracking task. The results show that although the current ADS-33C coupling criterion discriminates against those types of coupling typical of conventionally controlled helicopters, it is not always suited for the prediction of handling qualities of helicopters with modern control systems. Based on the observation that high frequency inputs during tracking are used to alleviate coupling, a frequency domain pitch-roll coupling criterion that uses the average coupling ratio between the bandwidth and neutral stability frequency is formulated. This criterion provides a more comprehensive coverage with respect to the different types of coupling, shows excellent consistency, and has the additional benefit that compliance testing data are obtained from the bandwidth/phase delay tests, so that no additional flight testing is needed.

RaD-X: Complementary measurements of dose rates at aviation altitudes

NASA Astrophysics Data System (ADS)

Meier, Matthias M.; Matthiä, Daniel; Forkert, Tomas; Wirtz, Michael; Scheibinger, Markus; Hübel, Robert; Mertens, Christopher J.

2016-09-01

The RaD-X stratospheric balloon flight organized by the National Aeronautics and Space Administration was launched from Fort Sumner on 25 September 2015 and carried several instruments to measure the radiation field in the upper atmosphere at the average vertical cutoff rigidity Rc of 4.1 GV. The German Aerospace Center (Deutsches Zentrum für Luft- und Raumfahrt) in cooperation with Lufthansa German Airlines supported this campaign with an independent measuring flight at the altitudes of civil aviation on a round trip from Germany to Japan. The goal was to measure dose rates under similar space weather conditions over an area on the Northern Hemisphere opposite to the RaD-X flight. Dose rates were measured in the target areas, i.e., around vertical cutoff rigidity Rc of 4.1 GV, at two flight altitudes for about 1 h at each position with acceptable counting statistics. The analysis of the space weather situation during the flights shows that measuring data were acquired under stable and moderate space weather conditions with a virtually undisturbed magnetosphere. The measured rates of absorbed dose in silicon and ambient dose equivalent complement the data recorded during the balloon flight. The combined measurements provide a set of experimental data suitable for validating and improving numerical models for the calculation of radiation exposure at aviation altitudes.

The First European Parabolic Flight Campaign with the Airbus A310 ZERO-G

NASA Astrophysics Data System (ADS)

Pletser, Vladimir; Rouquette, Sebastien; Friedrich, Ulrike; Clervoy, Jean-Francois; Gharib, Thierry; Gai, Frederic; Mora, Christophe

2016-12-01

Aircraft parabolic flights repetitively provide up to 23 seconds of reduced gravity during ballistic flight manoeuvres. Parabolic flights are used to conduct short microgravity investigations in Physical and Life Sciences and in Technology, to test instrumentation prior to space flights and to train astronauts before a space mission. The use of parabolic flights is complementary to other microgravity carriers (drop towers, sounding rockets), and preparatory to manned space missions on board the International Space Station and other manned spacecraft, such as Shenzhou and the future Chinese Space Station. After 17 years of using the Airbus A300 ZERO-G, the French company Novespace, a subsidiary of the ' Centre National d'Etudes Spatiales' (CNES, French Space Agency), based in Bordeaux, France, purchased a new aircraft, an Airbus A310, to perform parabolic flights for microgravity research in Europe. Since April 2015, the European Space Agency (ESA), CNES and the ` Deutsches Zentrum für Luft- und Raumfahrt e.V.' (DLR, the German Aerospace Center) use this new aircraft, the Airbus A310 ZERO-G, for research experiments in microgravity. The first campaign was a Cooperative campaign shared by the three agencies, followed by respectively a CNES, an ESA and a DLR campaign. This paper presents the new Airbus A310 ZERO-G and its main characteristics and interfaces for scientific experiments. The experiments conducted during the first European campaign are presented.

17th International Microgravity Measurements Group Meeting

NASA Technical Reports Server (NTRS)

DeLombard, Richard

1998-01-01

The Seventeenth International Microgravity Measurements Group (MGMG) meeting was held 24-26 March 1998 at the Ohio Aerospace Institute (OAI) in Brook Park, Ohio. This meeting focused on the transition of microgravity science research from the Shuttle, Mir, and free flyers to the International Space Station. The MGMG series of meetings are conducted by the Principal Investigator Microgravity Services project of the Microgravity Science Division at the NASA Lewis Research Center. The MGMG meetings provide a forum for the exchange of information and ideas about the microgravity environment and microgravity acceleration research in the Microgravity Research Program. The meeting had participation from investigators in all areas of microgravity research. The attendees included representatives from: NASA centers; National Space Development Agency of Japan; European Space Agency; Daimler Benz Aerospace AG; Deutsches Zentrum fuer Luft- und Raumfahrt; Centre National d'Etudes Spatiales; Canadian Space Agency, national research institutions; Universities in U.S., Italy, Germany, and Russia; and commercial companies in the U.S. and Russia. Several agencies presented summaries of the measurement, analysis, and characterization of the microgravity environment of the Shuttle, Mir, and sounding rockets over the past fifteen years. This extensive effort has laid a foundation for pursuing a similar course during future microgravity science experiment operations on the ISS. Future activities of microgravity environment characterization were discussed by several agencies who plan to operate on the ISS.

SOFIA: Flying the Telescope

NASA Technical Reports Server (NTRS)

Asher, Troy; Cumming, Steve

2012-01-01

The Stratospheric Observatory For Infrared Astronomy (SOFIA) is an international cooperative development and operations program between the United States National Aeronautics and Space Administration (NASA) and the German Space Agency, DLR (Deutsches Zentrum fuer Luft-und Raumfahrt). SOFIA is a 2.5 meter, optical/infrared/sub-millimeter telescope mounted in a Boeing model 747SP-21 aircraft and will be used for many basic astronomical observations performed at stratospheric altitudes. It will accommodate installation of different focal plane instruments with in-flight accessibility provided by investigators selected from the international science community. The Facility operational lifetime is planned to be greater than 20 years. This presentation will present the results of developmental testing of SOFIA, including analysis, envelope expansion and the first operational mission. It will describe a brief history of open cavities in flight, how NASA designed and tested SOFIAs cavity, as well as flight test results. It will focus on how the test team achieved key milestones by systematically and efficiently reducing the number of test points to only those absolutely necessary to achieve mission requirements, thereby meeting all requirements and saving the potential loss of program funding. Finally, it will showcase examples of the observatory in action and the first operational mission of the observatory, illustrating the usefulness of the system to the international scientific community. Lessons learned on how to whittle a mountain of test points into a manageable sum will be presented at the conclusion.

Sound attenuations of axial fan blade tones using flow-driven tunable resonator arrays

NASA Astrophysics Data System (ADS)

Gorny, Lee James

Flow-excited, tunable quarter-wavelength resonators can be integrated into the shrouds of ducted subsonic axial fans. This study explores their effectiveness in reducing propagations of tonal noise by means of acoustic wave cancellation. Resonators are a non-intrusive method of generating a secondary sound field near the plane of a rotor. As they can be strategically tuned to reduce radiated noise at the blade passage frequency (BPF) and its harmonics, resonators can be useful for a variety of applications to quiet existing and future turbomachinery. Experiments have demonstrated that a single quarter wave resonator is effective in reducing unidirectional plane wave propagations for long wavelength ducted applications while an array is effective for shorter wavelength or un-ducted facilities where shrouded fans are used. Testing conducted at Center for Acoustics and Vibrations (CAV) at the Pennsylvania State University the Deutsches Zentrum fur Luft und Raumfahrt (DLR) in Berlin, Germany demonstrated that resonator arrays were effective in attenuating shorter wavelength plane-wave and higher order modal propagations of blade tone noise. A chiller fan enclosure, constructed in the CAV laboratory emulated an industrial chiller in its operation. Using this facility, resonators were observed to attenuate blade tone noise from a non-ideal ducted geometry. The approaches used in this study evolved from Helmholtz resonators to conventional quarter wave tubes, to mouth tunable resonators, and finally to back-wall tunable resonators. These developments in tuning allowed for independent control of a resonator's magnitude and phase of the secondary sound field produced by the resonators. It was demonstrated that the use of two tunable resonator chambers oriented axially on either side of the blade region enables a dipole-like secondary sound field to be passively generated and bi-directional attenuations of plane wave noise to be achieved. Tonal attenuations of 28 dB were attained and BPF tones were reduced to less than 5 dB from the broadband noise floor for each case discussed above. In parallel with experimental work, analytical models were developed to effectively model and predict optimal resonator configurations for a given fan in operation. Interactions between resonators and the driving pressure field from the rotor blades are modeled using transmission line (TL) theory. Blade tone acoustic pressure is obtained using a finite element method (FEM) propagation code. By combining of these two methods, a resonator configuration that achieves optimal attenuation can be numerically obtained. The use of resonators has been shown to significantly attenuate fan noise in the conditions explored in the considered experiments. Numerical modeling has shown consistency in the response of flow driven resonators and their. These results indicate a strong potential for active control of fan noise using resonators and an approach to applying this control is presented.

Telemetry distribution and processing for the second German Spacelab Mission D-2

NASA Technical Reports Server (NTRS)

Rabenau, E.; Kruse, W.

1994-01-01

For the second German Spacelab Mission D-2 all activities related to operating, monitoring and controlling the experiments on board the Spacelab were conducted from the German Space Operations Control Center (GSOC) operated by the Deutsche Forschungsanstalt fur Luft- und Raumfahrt (DLR) in Oberpfaffenhofen, Germany. The operational requirements imposed new concepts on the transfer of data between Germany and the NASA centers and the processing of data at the GSOC itself. Highlights were the upgrade of the Spacelab Data Processing Facility (SLDPF) to real time data processing, the introduction of packet telemetry and the development of the high-rate data handling front end, data processing and display systems at GSOC. For the first time, a robot on board the Spacelab was to be controlled from the ground in a closed loop environment. A dedicated forward channel was implemented to transfer the robot manipulation commands originating from the robotics experiment ground station to the Spacelab via the Orbiter's text and graphics system interface. The capability to perform telescience from an external user center was implemented. All interfaces proved successful during the course of the D-2 mission and are described in detail in this paper.

A general theory of two- and three-dimensional rotational flow in subsonic and transonic turbomachines

NASA Technical Reports Server (NTRS)

Wu, Chung-Hua

1993-01-01

This report represents a general theory applicable to axial, radial, and mixed flow turbomachines operating at subsonic and supersonic speeds with a finite number of blades of finite thickness. References reflect the evolution of computational methods used, from the inception of the theory in the 50's to the high-speed computer era of the 90's. Two kinds of relative stream surfaces, S(sub 1) and S(sub 2), are introduced for the purpose of obtaining a three-dimensional flow solution through the combination of two-dimensional flow solutions. Nonorthogonal curvilinear coordinates are used for the governing equations. Methods of computing transonic flow along S(sub 1) and S(sub 2) stream surfaces are given for special cases as well as for fully three-dimensional transonic flows. Procedures pertaining to the direct solutions and inverse solutions are presented. Information on shock wave locations and shapes needed for computations are discussed. Experimental data from a Deutsche Forschungs- und Versuchsanstalt fur Luft- und Raumfahrt e.V. (DFVLR) rotor and from a Chinese Academy of Sciences (CAS) transonic compressor rotor are compared with the computed flow properties.

Expression of transcription factors after short-term exposure of Arabidopsis thaliana cell cultures to hyper-g, and to simulated and sounding rocket micro-g

NASA Astrophysics Data System (ADS)

Hampp, R.; Babbick, M.

Previous microarray studies with cell cultures of Arabidopsis thaliana cv Columbia have shown responses in gene expression which were partly specific to exposure to microgravity sounding rocket experiment TEXUS In order to get access to early responses upon changes in gravitational fields we used exposure times as short as 2 min For this purpose we selected a range of genes which code for different groups of transcription factors WRKY ERF MYB MADS Samples were taken in 5-min clinorotation 2- and 3-dimensional hypergravity 8g and 2-min intervals sounding rocket experiment Amounts of transcripts were determined by quantitative RT PCR Most transcripts showed a significant transient change in content within a time frame of up to 30 min after changing the external gravitational field strength They could be grouped into 1 basic stress responses which occurred under all conditions 2 clinorotation-related effects which were either identical or opposite between 2D 60 rpm 4x10 -2 g and 3D clinorotation random positioning machine and 3 alterations specific to the microgravity exposure under sounding rocket conditions MAXUS The data are discussed in relation to gravitation-dependent signalling chains and with regard to the simulation of microgravity by means of clinorotation Supported by a grant from the Deutsches Zentrum f u r Luft- und Raumfahrt e V grant no 50 WB 0143

Identification and characterization of individual airborne volcanic ash particles by Raman microspectroscopy.

PubMed

Ivleva, Natalia P; Huckele, Susanne; Weinzierl, Bernadett; Niessner, Reinhard; Haisch, Christoph; Baumann, Thomas

2013-11-01

We present for the first time the Raman microspectroscopic identification and characterization of individual airborne volcanic ash (VA) particles. The particles were collected in April/May 2010 during research aircraft flights, which were performed by Deutsches Zentrum für Luft- und Raumfahrt in the airspace near the Eyjafjallajökull volcano eruption and over Europe (between Iceland and Southern Germany). In addition, aerosol particles were sampled by an Electrical Low Pressure Impactor in Munich, Germany. As references for the Raman analysis, we used the spectra of VA collected at the ground near the place of eruption, of mineral basaltic rock, and of different minerals from a database. We found significant differences in the spectra of VA and other aerosol particles (e.g., soot, nitrates, sulfates, and clay minerals), which allowed us to identify VA among other atmospheric particulate matter. Furthermore, while the airborne VA shows a characteristic Raman pattern (with broad band from ca. 200 to ca. 700 cm(-1) typical for SiO₂ glasses and additional bands of ferric minerals), the differences between the spectra of aged and fresh particles were observed, suggesting differences in their chemical composition and/or structure. We also analyzed similarities between Eyjafjallajökull VA particles collected at different sampling sites and compared the particles with a large variety of glassy and crystalline minerals. This was done by applying cluster analysis, in order to get information on the composition and structure of volcanic ash.

A new AS-display as part of the MIRO lightweight robot for surgical applications

NASA Astrophysics Data System (ADS)

Grossmann, Christoph M.

2010-02-01

The DLR MIRO is the second generation of versatile robot arms for surgical applications, developed at the Institute for Robotics and Mechatronics at Deutsche Zentrum für Luft- und Raumfahrt (DLR) in Oberpfaffenhofen, Germany. With its low weight of 10 kg and dimensions similar to those of the human arm, the MIRO robot can assist the surgeon directly at the operating table where space is scarce. The planned scope of applications of this robot arm ranges from guiding a laser unit for the precise separation of bone tissue in orthopedics to positioning holes for bone screws, robot assisted endoscope guidance and on to the multi-robot concept for endoscopic minimally invasive surgery. A stereo-endoscope delivers two full HD video streams that can even be augmented with information, e.g vectors indicating the forces that act on the surgical tool at any given moment. SeeFront's new autostereoscopic 3D display SF 2223, being a part of the MIRO assembly, will let the surgeon view the stereo video stream in excellent quality, in real time and without the need for any viewing aids. The presentation is meant to provide an insight into the principles at the basis of the SeeFront 3D technology and how they allow the creation of autostereoscopic display solutions ranging from smallest "stamp-sized" displays to 30" desktop versions, which all provide comfortable freedom of movement for the viewer along with excellent 3D image quality.

77 FR 32887 - Airworthiness Directives; Burkhart GROB Luft- und Raumfahrt GmbH Powered Sailplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-04

... Airworthiness Directives; Burkhart GROB Luft- und Raumfahrt GmbH Powered Sailplanes AGENCY: Federal Aviation... seaside and therefore exposed to a salty environment, causing the excessive corrosion. This condition, if... Programs,'' describes in more detail the scope of the Agency's authority. We are issuing this rulemaking...

The Great Unescape: Three Mile Island, Fukushima, and Beyond

ERIC Educational Resources Information Center

Spector, Hannah

2015-01-01

During the Second World War, the Luftwaffe ran a maximum security prisoner of war (POW) camp called "Stalag Luft III," which imprisoned captured Allied air force servicemen. The story of the 1944 escape from Stalag Luft III is one of the most famous stories of the Second World War as described in the firsthand written account and…

Performance Simulations for a Spaceborne Methane Lidar Mission

NASA Technical Reports Server (NTRS)

Kiemle, C.; Kawa, Stephan Randolph; Quatrevalet, Mathieu; Browell, Edward V.

2014-01-01

Future spaceborne lidar measurements of key anthropogenic greenhouse gases are expected to close current observational gaps particularly over remote, polar, and aerosol-contaminated regions, where actual in situ and passive remote sensing observation techniques have difficulties. For methane, a "Methane Remote Lidar Mission" was proposed by Deutsches Zentrum fuer Luft- und Raumfahrt and Centre National d'Etudes Spatiales in the frame of a German-French climate monitoring initiative. Simulations assess the performance of this mission with the help of Moderate Resolution Imaging Spectroradiometer and Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observations of the earth's surface albedo and atmospheric optical depth. These are key environmental parameters for integrated path differential absorption lidar which uses the surface backscatter to measure the total atmospheric methane column. Results showthat a lidar with an average optical power of 0.45W at 1.6 µm wavelength and a telescope diameter of 0.55 m, installed on a low Earth orbit platform(506 km), will measure methane columns at precisions of 1.2%, 1.7%, and 2.1% over land, water, and snow or ice surfaces, respectively, for monthly aggregated measurement samples within areas of 50 × 50 km2. Globally, the mean precision for the simulated year 2007 is 1.6%, with a standard deviation of 0.7%. At high latitudes, a lower reflectance due to snow and ice is compensated by denser measurements, owing to the orbital pattern. Over key methane source regions such as densely populated areas, boreal and tropical wetlands, or permafrost, our simulations show that the measurement precision will be between 1 and 2%.

SUMIRAD: a near real-time MMW radiometer imaging system for threat detection in an urban environment

NASA Astrophysics Data System (ADS)

Dill, Stephan; Peichl, Markus; Rudolf, Daniel

2012-10-01

The armed forces are nowadays confronted with a wide variety of types of operations. During peace keeping missions in an urban environment, where small units patrol the streets with armored vehicles, the team leader is confronted with a very complex threat situation. The asymmetric imminence arises in most cases from so called IEDs (Improvised explosive devices) which are found in a multitude of versions. In order to avoid risky situations the early detection of possible threats due to advanced reconnaissance and surveillance sensors will provide an important advantage. A European consortium consisting of GMV S.A. (Spain, "Grupo Tecnològico e Industrial"), RMA (Belgium, "Royal Military Academy"), TUM ("Technische Universität München") and DLR (Germany, "Deutsches Zentrum für Luft- und Raumfahrt") developed in the SUM project (Surveillance in an urban environment using mobile sensors) a low-cost multi-sensor vehicle based surveillance system in order to enhance situational awareness for moving security and military patrols as well as for static checkpoints. The project was funded by the European Defense Agency (EDA) in the Joint Investment Program on Force Protection (JIP-FP). The SUMIRAD (SUM imaging radiometer) system, developed by DLR, is a fast radiometric imager and part of the SUM sensor suite. This paper will present the principle of the SUMIRAD system and its key components. Furthermore the image processing will be described. Imaging results from several measurement campaigns will be presented. The overall SUM system and the individual subsystems are presented in more detail in separate papers during this conference.

Ulrich C. Luft and physiology on Nanga Parbat: the winds of war.

PubMed

Rodway, George W

2009-01-01

Rodway, George W. Ulrich C. Luft and physiology on Nanga Parbat: the winds of war. High Alt. Med. Biol. 10:89-96, 2009.-Ulrich Cameron Luft (1910-1991) is a significant figure in the annals of high altitude physiology and medicine. He combined a passionate interest in mountaineering with an equally passionate interest in human physiology at a pivotal time in modern history. His involvement in the 1937 and 1938 German Nanga Parbat expeditions as mountaineer and scientist set the stage for his subsequent work in aviation physiology carried out in Germany and then later in America when aerospace medicine was emerging as a specialty. His postwar career as a scientist and educator was equally distinguished by virtue of the large numbers of young scientists he trained and his contributions to aerospace medicine over the course of several decades.

16 CFR 301.44 - Misrepresentation of prices.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) No person shall, with respect to a fur or fur product, advertise such fur or fur product at alleged... shall any person advertise a fur or fur product at prices purported to be reduced from what are in fact.... (b) No person shall, with respect to a fur or fur product, advertise such fur or fur product with...

Assessment of four methods to estimate surface UV radiation using satellite data, by comparison with ground measurements from four stations in Europe

NASA Astrophysics Data System (ADS)

Arola, Antti; Kalliskota, S.; den Outer, P. N.; Edvardsen, K.; Hansen, G.; Koskela, T.; Martin, T. J.; Matthijsen, J.; Meerkoetter, R.; Peeters, P.; Seckmeyer, G.; Simon, P. C.; Slaper, H.; Taalas, P.; Verdebout, J.

2002-08-01

Four different satellite-UV mapping methods are assessed by comparing them against ground-based measurements. The study includes most of the variability found in geographical, meteorological and atmospheric conditions. Three of the methods did not show any significant systematic bias, except during snow cover. The mean difference (bias) in daily doses for the Rijksinstituut voor Volksgezondheid en Milieu (RIVM) and Joint Research Centre (JRC) methods was found to be less than 10% with a RMS difference of the order of 30%. The Deutsches Zentrum für Luft- und Raumfahrt (DLR) method was assessed for a few selected months, and the accuracy was similar to the RIVM and JRC methods. It was additionally used to demonstrate how spatial averaging of high-resolution cloud data improves the estimation of UV daily doses. For the Institut d'Aéronomie Spatiale de Belgique (IASB) method the differences were somewhat higher, because of their original cloud algorithm. The mean difference in daily doses for IASB was about 30% or more, depending on the station, while the RMS difference was about 60%. The cloud algorithm of IASB has been replaced recently, and as a result the accuracy of the IASB method has improved. Evidence is found that further research and development should focus on the improvement of the cloud parameterization. Estimation of daily exposures is likely to be improved if additional time-resolved cloudiness information is available for the satellite-based methods. It is also demonstrated that further development work should be carried out on the treatment of albedo of snow-covered surfaces.

NASA Alternative-Fuel Effects on Contrails and Cruise Emissions (ACCESS) Flight Experiments

NASA Astrophysics Data System (ADS)

Anderson, B. E.; Moore, R.; Beyersdorf, A. J.; Thornhill, K. L., II; Shook, M.; Winstead, E.; Ziemba, L. D.; Bulzan, D. L.; Brown, A.; Beaton, B.; Schlager, H.

2014-12-01

Although the emission performance of gas-turbine engines burning renewable aviation fuels have been thoroughly documented in recent ground-based studies, there is still great uncertainty regarding how the fuels effect aircraft exhaust composition and contrail formation at cruise altitudes. To fill this information gap, the NASA Aeronautics Research Mission Directorate sponsored the ACCESS flight series to make detailed measurements of trace gases, aerosols and ice particles in the near-field behind the NASA DC-8 aircraft as it burned either standard petroleum-based fuel of varying sulfur content or a 50:50 blend of standard fuel and a hydro-treated esters and fatty acid (HEFA) jet fuel produced from camelina plant oil. ACCESS 1, conducted in spring 2013 near Palmdale CA, focused on refining flight plans and sampling techniques and used the instrumented NASA Langley HU-25 aircraft to document DC-8 emissions and contrails on five separate flights of ~2 hour duration. ACCESS 2, conducted from Palmdale in May 2014, engaged partners from the Deutsches Zentrum für Luft- und Raumfahrt (DLR) and National Research Council-Canada to provide additional scientific expertise and sampling aircraft (Falcon 20 and CT-133, respectively) with more extensive trace gas, particle, or air motion measurement capability. Eight, muliti-aircraft research flights of 2 to 4 hour duration were conducted to document the emissions and contrail properties of the DC-8 as it 1) burned low sulfur Jet A, high sulfur Jet A or low sulfur Jet A/HEFA blend, 2) flew at altitudes between 6 and 11 km, and 3) operated its engines at three different fuel flow rates. This presentation further describes the ACCESS flight experiments, examines fuel type and thrust setting impacts on engine emissions, and compares cruise-altitude observations with similar data acquired in ground-test venues.

Alternative-Fuel Effects on Contrails & Cruise Emissions (ACCESS-2) Flight Experiment

NASA Technical Reports Server (NTRS)

Anderson, Bruce E.

2015-01-01

Although the emission performance of gas-turbine engines burning renewable aviation fuels have been thoroughly documented in recent ground-based studies, there is still great uncertainty regarding how the fuels effect aircraft exhaust composition and contrail formation at cruise altitudes. To fill this information gap, the NASA Aeronautics Research Mission Directorate sponsored the ACCESS flight series to make detailed measurements of trace gases, aerosols and ice particles in the near-field behind the NASA DC-8 aircraft as it burned either standard petroleum-based fuel of varying sulfur content or a 50:50 blend of standard fuel and a hydro-treated esters and fatty acid (HEFA) jet fuel produced from camelina plant oil. ACCESS 1, conducted in spring 2013 near Palmdale CA, focused on refining flight plans and sampling techniques and used the instrumented NASA Langley HU-25 aircraft to document DC-8 emissions and contrails on five separate flights of approx.2 hour duration. ACCESS 2, conducted from Palmdale in May 2014, engaged partners from the Deutsches Zentrum fuer Luft- und Raumfahrt (DLR) and National Research Council-Canada to provide additional scientific expertise and sampling aircraft (Falcon 20 and CT-133, respectively) with more extensive trace gas, particle, or air motion measurement capability. Eight, muliti-aircraft research flights of 2 to 4 hour duration were conducted to document the emissions and contrail properties of the DC-8 as it 1) burned low sulfur Jet A, high sulfur Jet A or low sulfur Jet A/HEFA blend, 2) flew at altitudes between 6 and 11 km, and 3) operated its engines at three different fuel flow rates. This presentation further describes the ACCESS flight experiments, examines fuel type and thrust setting impacts on engine emissions, and compares cruise-altitude observations with similar data acquired in ground tests.

The AMINO experiment: exposure of amino acids in the EXPOSE-R experiment on the International Space Station and in laboratory

NASA Astrophysics Data System (ADS)

Bertrand, Marylène; Chabin, Annie; Colas, Cyril; Cadène, Martine; Chaput, Didier; Brack, Andre; Cottin, Herve

2015-01-01

In order to confirm the results of previous experiments concerning the chemical behaviour of organic molecules in the space environment, organic molecules (amino acids and a dipeptide) in pure form and embedded in meteorite powder were exposed in the AMINO experiment in the EXPOSE-R facility onboard the International Space Station. After exposure to space conditions for 24 months (2843 h of irradiation), the samples were returned to the Earth and analysed in the laboratory for reactions caused by solar ultraviolet (UV) and other electromagnetic radiation. Laboratory UV exposure was carried out in parallel in the Cologne DLR Center (Deutsches Zentrum für Luft und Raumfahrt). The molecules were extracted from the sample holder and then (1) derivatized by silylation and analysed by gas chromatography coupled to a mass spectrometer (GC-MS) in order to quantify the rate of degradation of the compounds and (2) analysed by high-resolution mass spectrometry (HRMS) in order to understand the chemical reactions that occurred. The GC-MS results confirm that resistance to irradiation is a function of the chemical nature of the exposed molecules and of the wavelengths of the UV light. They also confirm the protective effect of a coating of meteorite powder. The most altered compounds were the dipeptides and aspartic acid while the most robust were compounds with a hydrocarbon chain. The MS analyses document the products of reactions, such as decarboxylation and decarbonylation of aspartic acid, taking place after UV exposure. Given the universality of chemistry in space, our results have a broader implication for the fate of organic molecules that seeded the planets as soon as they became habitable as well as for the effects of UV radiation on exposed molecules at the surface of Mars, for example.

A green observatory in the Chilean Atacama desert

NASA Astrophysics Data System (ADS)

Ramolla, Michael; Westhues, Christian; Hackstein, Moritz; Haas, Martin; Hodapp, Klaus; Lemke, Roland; Barr Domínguez, Angie; Chini, Rolf; Murphy, Miguel

2016-08-01

Since 2007, the Ruhr-Universität Bochum (RUB) in Germany and Universidad Católica del Norte (UCN) in Chile jointly operate the Universitätssternwarte der Ruhr-Universität Bochum (USB), which is located in direct neighborhood of the future E-ELT of ESO. It is the only observatory powered exclusively by solar panels and wind turbines. Excess power is stored in batteries that allow uninterrupted operation even in windless nights. The scientific equipment consists of three robotic optical telescopes with apertures ranging from 15 cm (RoBoTT) over 25 cm (BESTII) to 40 cm (BMT) and one 80 cm (IRIS) infra-red telescope. The optical telescopes are equipped with Johnson and Sloan broad band filters together with a large number of narrow and intermediate bands. In the infrared, J,H and K filters are available, accompanied by several narrow bands near the K band wavelength. The second Nasmyth focus in the 80 cm telescope feeds a high resolution echelle spectrograph similar to the FEROS instrument of ESO. This variety of instruments has evolved from different collaborations, i.e. with the University of Hawaii (IfA) in the USA, which provided the near-infrared-camera of the IRIS telescope, or with the Deutsches Zentrum für Luft- und Raumfahrt (DLR) in Germany, which provided the BESTII telescope. The highly automatized processes on all telescopes enable a single person to run the whole facility, providing the high cost efficiency required for an university observatory. The excellent site conditions allow projects that require daily observations of astronomical objects over epochs of several months or years. Here we report on such studies of young stellar objects from the Bochum Galactic Disk Survey, the multiplicity of stars, quasar variability or the hunt for exo-planets.

Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator

NASA Astrophysics Data System (ADS)

Deng, Zengqin; Wang, Qing; Liu, Zhao; Zhang, Manfeng; Machado, Ana Carolina Dantas; Chiu, Tsu-Pei; Feng, Chong; Zhang, Qi; Yu, Lin; Qi, Lei; Zheng, Jiangge; Wang, Xu; Huo, Xinmei; Qi, Xiaoxuan; Li, Xiaorong; Wu, Wei; Rohs, Remo; Li, Ying; Chen, Zhongzhou

2015-07-01

Ferric uptake regulator (Fur) plays a key role in the iron homeostasis of prokaryotes, such as bacterial pathogens, but the molecular mechanisms and structural basis of Fur-DNA binding remain incompletely understood. Here, we report high-resolution structures of Magnetospirillum gryphiswaldense MSR-1 Fur in four different states: apo-Fur, holo-Fur, the Fur-feoAB1 operator complex and the Fur-Pseudomonas aeruginosa Fur box complex. Apo-Fur is a transition metal ion-independent dimer whose binding induces profound conformational changes and confers DNA-binding ability. Structural characterization, mutagenesis, biochemistry and in vivo data reveal that Fur recognizes DNA by using a combination of base readout through direct contacts in the major groove and shape readout through recognition of the minor-groove electrostatic potential by lysine. The resulting conformational plasticity enables Fur binding to diverse substrates. Our results provide insights into metal ion activation and substrate recognition by Fur that suggest pathways to engineer magnetotactic bacteria and antipathogenic drugs.

FurA contributes to the oxidative stress response regulation of Mycobacterium avium ssp. paratuberculosis

PubMed Central

Eckelt, Elke; Meißner, Thorsten; Meens, Jochen; Laarmann, Kristin; Nerlich, Andreas; Jarek, Michael; Weiss, Siegfried; Gerlach, Gerald-F.; Goethe, Ralph

2015-01-01

The ferric uptake regulator A (FurA) is known to be involved in iron homeostasis and stress response in many bacteria. In mycobacteria the precise role of FurA is still unclear. In the presented study, we addressed the functional role of FurA in the ruminant pathogen Mycobacterium avium ssp. paratuberculosis (MAP) by construction of a furA deletion strain (MAPΔfurA). RNA deep sequencing revealed that the FurA regulon consists of repressed and activated genes associated to stress response or intracellular survival. Not a single gene related to metal homeostasis was affected by furA deletion. A decisive role of FurA during intracellular survival in macrophages was shown by significantly enhanced survival of MAPΔfurA compared to the wildtype, indicating that a principal task of mycobacterial FurA is oxidative stress response regulation in macrophages. This resistance was not associated with altered survival of mice after long term infection with MAP. Our results demonstrate for the first time, that mycobacterial FurA is not involved in the regulation of iron homeostasis. However, they provide strong evidence that FurA contributes to intracellular survival as an oxidative stress sensing regulator. PMID:25705205

FurA contributes to the oxidative stress response regulation of Mycobacterium avium ssp. paratuberculosis.

PubMed

Eckelt, Elke; Meißner, Thorsten; Meens, Jochen; Laarmann, Kristin; Nerlich, Andreas; Jarek, Michael; Weiss, Siegfried; Gerlach, Gerald-F; Goethe, Ralph

2015-01-01

The ferric uptake regulator A (FurA) is known to be involved in iron homeostasis and stress response in many bacteria. In mycobacteria the precise role of FurA is still unclear. In the presented study, we addressed the functional role of FurA in the ruminant pathogen Mycobacterium avium ssp. paratuberculosis (MAP) by construction of a furA deletion strain (MAPΔfurA). RNA deep sequencing revealed that the FurA regulon consists of repressed and activated genes associated to stress response or intracellular survival. Not a single gene related to metal homeostasis was affected by furA deletion. A decisive role of FurA during intracellular survival in macrophages was shown by significantly enhanced survival of MAPΔfurA compared to the wildtype, indicating that a principal task of mycobacterial FurA is oxidative stress response regulation in macrophages. This resistance was not associated with altered survival of mice after long term infection with MAP. Our results demonstrate for the first time, that mycobacterial FurA is not involved in the regulation of iron homeostasis. However, they provide strong evidence that FurA contributes to intracellular survival as an oxidative stress sensing regulator.

16 CFR 301.22 - Disclosure of damaged furs.

Code of Federal Regulations, 2011 CFR

2011-01-01

... decrease the normal life and durability of such product. (b) When damaged furs are used in a fur product..., or advertising such product; as for example: Mink Fur origin: Canada Contains Damaged Fur ...

Managed care plan performance since 1980: another look at 2 literature reviews.

PubMed Central

Sullivan, K

1999-01-01

OBJECTIVES: This article compares the quality of care provided by managed care plans (MCPs) and indemnity (or fee-for-service [FFS]) plans since 1980. METHODS: The 44 studies examined are the studies that Miller and Luft cited in their 1994 and 1997 reviews of the literature comparing MCPs with FFS plans. These studies are examined to determine how well they met Miller and Luft's selection criteria and, in addition, whether they controlled for differences in the breadth of insurance coverage. RESULTS: The 44 studies generated 57 observations. MCPs scored better than FFS plans on 10 of these, equally well on 25, and worse on 22. However, only 44 of these observations met the Miller-Luft criteria plus the coverage criterion. Four of these indicated that MCP care was better, 19 that MCP and FFS care were equivalent, and 21 that MCP care was worse. CONCLUSIONS: The small body of reliable studies comparing the quality of MCP care with that of FFS care indicates that the quality of care provided by MCPs tends to be equal or inferior to that provided by FFS plans. PMID:10394307

Diagnosis of upper urinary tract tumours: is photodynamic diagnosis assisted ureterorenoscopy required as an addition to modern imaging and ureterorenoscopy?

PubMed

Aboumarzouk, Omar M; Mains, Edward; Moseley, Harry; Kata, Sławomir G

2013-05-01

We aimed to assess the diagnostic accuracy of photodynamic diagnostic ureterorenoscopy (PDD-FURS) in detection of UUT-TCC in comparison with CT Urogram (CTU) and WL-FURS. Between June 2009 and August 2011, 30 patients underwent PDD-FURS after CTU for suspicion of UUT-TCC. Ureterorenoscopy was performed for abnormal upper urinary tract on imaging. Oral 5-Aminolevulinic Acid (5-ALA) was used as a photosensitizer. All procedures were performed by single endourologist experienced in photodynamic diagnosis. The sensitivity, specificity, and detection rate of WL-FURS, PDD-FURS and CTU were calculated using the Meta-DiSc v1.4 programme. P values <0.05 were considered significant. PDD-FURS detected more UUT-TCCs than CTU or WL-FURS (94% (16/17) vs. 76.5% (13/17) vs. 82% (14/17) respectively). PDD-FURS was not significantly more sensitive than CTU and WL-FURS to detect UUT-TCC (0.94 (95% CI: 0.71-0.99) vs. 0.82 (95% CI: 0.57-0.96) vs. 0.81 (95% CI: 0.54-0.96) respectively; PDD-FURS vs. CTU: P=0.249; PDD-FURS vs. WL-FURS: P=0.277). There was no difference in the specificity between PDD-FURS and WL-FURS (1.0 (95% CI: 0.75-1.0) and 1.0 (95% CI: 0.75-1.0) respectively) (P=1), while PDD-FURS was significantly more specific than CTU (CTU: 0.21 (95% CI: 0.05-0.51) (P<0.001). PDD-FURS picked up 3 cases of CIS, which was not seen on WL-FURL and CTU. Oral 5-ALA induced PDD-FURS has a high sensitivity and specificity to detect lesions and a higher detection rate to diagnose UUT-TCC than WL-FURS and CTU. It appears to be the only tool to visualise UUT CIS lesions. Copyright © 2012 Elsevier B.V. All rights reserved.

16 CFR 301.36 - Sectional fur products.

Code of Federal Regulations, 2010 CFR

2010-01-01

...; as for example: Dyed Rabbit Fur origin: France Trimming: Dyed Mouton-processed Lamb Fur origin... different animal furs added to complete a fur product or skin such as the ears, snoot, or under part of the...

29 CFR 780.124 - Raising of fur-bearing animals.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 3 2014-07-01 2014-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur of...

29 CFR 780.124 - Raising of fur-bearing animals.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 3 2013-07-01 2013-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur of...

29 CFR 780.124 - Raising of fur-bearing animals.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur of...

29 CFR 780.124 - Raising of fur-bearing animals.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 3 2012-07-01 2012-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur of...

29 CFR 780.124 - Raising of fur-bearing animals.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 3 2011-07-01 2011-07-01 false Raising of fur-bearing animals. 780.124 Section 780.124... General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780.124 Raising of fur-bearing animals. (a) The term “fur-bearing animals” has reference to animals which bear fur of...

Photoperiod and fur lengths in the arctic fox ( Alopex lagopus L.)

NASA Astrophysics Data System (ADS)

Underwood, L. S.; Reynolds, Patricia

1980-03-01

Pelage is seasonally dimorphic in the Arctic fox. During the winter, fur lengths for this species are nearly double similar values taken during the summer season. Considerable site-specific differences in fur length are noted. In general, body sites which are exposed to the environment when an Arctic fox lies in a curled position show greater fur lengths in all seasons and greater seasonal variations than body sites that are more protected during rest. Well-furred sites may tend to conserve heat during periods of inactivity, and scantily furred sites may tend to dissipate heat during periods of exercise. The growth of winter fur may compensate for the severe cold of the arctic winter. Changes in fur lengths indicate a definite pattern in spite of individual variations. During the fall months, fur lengths seem to lag behind an increasing body-to-ambient temperature gradient. Both body-to-ambient temperature gradients and fur lengths peak during December through February. From March through June, gradual environmental warming is accompanied by a decrease in average fur lengths. Thus, there appears to be a remarkable parallel between the body-to-ambient temperature gradient and the fur lengths. The growth of fur in the Arctic fox parallels annual changes in ambient temperature and photoperiod.

16 CFR 301.12 - Country of origin of imported furs.

Code of Federal Regulations, 2011 CFR

2011-01-01

... labeling shall be preceded by the term fur origin; as for example: Dyed Muskrat Fur Origin: Russia or Dyed... example: Tip-dyed Canadian American Sable Fur Origin: Canada or Russian Sable Fur Origin: Russia (f...

16 CFR 301.12 - Country of origin of imported furs.

Code of Federal Regulations, 2014 CFR

2014-01-01

... labeling shall be preceded by the term fur origin; as for example: Dyed Muskrat Fur Origin: Russia or Dyed... example: Tip-dyed Canadian American Sable Fur Origin: Canada or Russian Sable Fur Origin: Russia (f...

16 CFR 301.12 - Country of origin of imported furs.

Code of Federal Regulations, 2012 CFR

2012-01-01

... labeling shall be preceded by the term fur origin; as for example: Dyed Muskrat Fur Origin: Russia or Dyed... example: Tip-dyed Canadian American Sable Fur Origin: Canada or Russian Sable Fur Origin: Russia (f...

16 CFR 301.12 - Country of origin of imported furs.

Code of Federal Regulations, 2013 CFR

2013-01-01

... labeling shall be preceded by the term fur origin; as for example: Dyed Muskrat Fur Origin: Russia or Dyed... example: Tip-dyed Canadian American Sable Fur Origin: Canada or Russian Sable Fur Origin: Russia (f...

16 CFR 301.12 - Country of origin of imported furs.

Code of Federal Regulations, 2010 CFR

2010-01-01

... labeling shall be preceded by the term fur origin; as for example: Dyed Muskrat Fur Origin: Russia or Dyed... example: Tip-dyed Canadian American Sable Fur Origin: Canada or Russian Sable Fur Origin: Russia (f...

A pilot study on the correlation of tongue manifestation with the site of cerebral infarction in patients with stroke.

PubMed

Liu, Ping; Gao, Li; Song, Jue-Xian; Zhao, Hai-Ping; Wu, Xiao-Guang; Xu, Chang-Min; Huang, Li-Yuan; Wang, Ping-Ping; Luo, Yu-Min

2014-11-01

To discuss the correlation of tongue manifestation with the site of cerebral infarction in patients with acute cerebral infarction. From March 2008 to February 2009, 200 cases of hospitalized patients with first unilateral cerebral infarction were chosen in the Department of Neurology, Xuanwu Hospital. The correlation of different tongue color, fur texture, fur color with the site of cerebral infarction was analyzed. The site of cerebral infarction in patients were compared between different tongue color by Chisquare test (P=0.314), and further correspondence analysis demonstrated that there was correlation between red tongue and cortical-subcortical infarction group. The site of cerebral infarction in patients were compared between thick fur group and thin fur group, cortical-subcortical infarction occurred more frequently in the former (P=0.0008). The site of cerebral infarction in patients were compared between dry fur group, moist fur group and smooth fur group, correspondence analysis demonstrated there was correlation between dry fur and cortical-subcortical group. The site of cerebral infarction in the patients were compared between white fur group, white-yellow fur group and yellow fur group (P=0.010), and correspondence analysis demonstrated there was correlation between white fur and brainstem infarction; white-yellow fur has relationship with cortical infarction; subcortical infarction was weakly related with white-yellow fur; there was closer relationship between yellow fur and cortical-subcortical infarction. The change of tongue manifestation was associated with the site of cerebral infarction in patients, providing a new combining site for diagnosing cerebrovascular diseases by integrative medicine.

Mortality among retired fur workers. Dyers, dressers (tanners) and service workers.

PubMed

Sweeney, M H; Walrath, J; Waxweiler, R J

1985-08-01

A retrospective cohort mortality study was conducted on 807 fur dyers, fur dressers (tanners), and fur service workers who were pensioned between 1952 and 1977 by the Fur, Leather and Machine Workers Union of New York City. Workplace exposures of fur workers varied with job category. Dyers were exposed to oxidative dyes used in commercial hair dyes; dressers and service workers were exposed to tanning chemicals. In a comparison with the New York City population, no significant increases in mortality were observed among the fur dyers. Among fur dressers, mortality from all malignant neoplasms [standardized mortality ratio (SMR) 151] and lung cancer (SMR 232) was significantly elevated, as was mortality from cardiovascular disease (SMR 126) among fur service workers. When examined by ethnic origin, the elevated SMR values and directly age-adjusted rate ratios suggested that foreign-born fur dressers and eastern European-born fur workers experienced the highest risks for lung and colorectal cancers, respectively. These data support previous findings of increased mortality from colorectal cancer in the foreign-born population of the United States and suggest a possible occupational etiology for the observed lung cancer excess.

Outcomes of flexible ureterorenoscopy and laser fragmentation for renal stones: comparison between digital and conventional ureteroscope.

PubMed

Somani, Bhaskar K; Al-Qahtani, Saeed M; de Medina, Sixtina Diez Gil; Traxer, Olivier

2013-11-01

To compare the outcomes of flexible ureterorenoscopy and lasertripsy (FURS) using digital and conventional FURS for kidney stones. From September 2007 to April 2011, 118 patients underwent FURS (by the same surgeon). The outcomes were compared between equal numbers of procedures (59 each) using a conventional flexible ureterorenoscope (C-FURS; Olympus URF-P5) and a digital flexible ureterorenoscope (D-FURS; Olympus URF-V). Although the deflection, working channel, and field view are similar in both, the initial and terminal diameter is 8.4F and 9.9F and 6.9F and 8.4F for the D-FURS and C-FURS, respectively. The mean stone fragmentation time was calculated by the size per operative time. The preoperative, operative, and postoperative data were retrospectively analyzed and compared. The patient demographics were comparable. The mean stone size was 12.8 and 12 mm in the C-FURS and D-FURS groups, respectively. The initial assessment of the entire pyelocaliceal system was possible in 58 of 59 cases (98%) in the C-FURS group and 56 of 59 cases (94%) in the D-FURS group. The mean operative time was significantly longer in the C-FURS group (53.8 ± 15.2 minutes vs 44.5 ± 14.9 minutes). The overall stone-free rate 1 month after the procedure was 86% in the C-FURS group and 88% in the D-FURS group. Although on comparison, the D-FURS had slightly limited maneuverability, comparable success rates can be achieved with both conventional and digital ureteroscopes. D-FURSs significantly reduced the operative time compared with C-FURSs. Copyright © 2013 Elsevier Inc. All rights reserved.

Analysis of a Ferric Uptake Regulator (Fur) Mutant ofDesulfovibrio vulgaris Hildenborough

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bender, Kelly S.; Yen, Huei-Che Bill; Hemme, Christopher L.

2007-09-21

Previous experiments examining the transcriptional profileof the anaerobe Desulfovibrio vulgaris demonstrated up-regulation of theFur regulon in response to various environmental stressors. To test theinvolvement of Fur in the growth response and transcriptional regulationof D. vulgaris, a targeted mutagenesis procedure was used for deletingthe fur gene. Growth of the resulting ?fur mutant (JW707) was notaffected by iron availability, but the mutant did exhibit increasedsensitivity to nitrite and osmotic stresses compared to the wild type.Transcriptional profiling of JW707 indicated that iron-bound Fur acts asa traditional repressor for ferrous iron uptake genes (feoAB) and othergenes containing a predicted Fur binding site within theirmore » promoter.Despite the apparent lack of siderophore biosynthesis genes within the D.vulgaris genome, a large 12-gene operon encoding orthologs to TonB andTolQR also appeared to be repressed by iron-bound Fur. While other genespredicted to be involved in iron homeostasis were unaffected by thepresence or absence of Fur, alternative expression patterns that could beinterpreted as repression or activation by iron-free Fur were observed.Both the physiological and transcriptional data implicate a globalregulatory role for Fur in the sulfate-reducing bacterium D.vulgaris.« less

Bacillus licheniformis Contains Two More PerR-Like Proteins in Addition to PerR, Fur, and Zur Orthologues

PubMed Central

Ju, Shin-Yeong; Yang, Yoon-Mo; Ryu, Su-Hyun; Kwon, Yumi; Won, Young-Bin; Lee, Yeh-Eun; Youn, Hwan; Lee, Jin-Won

2016-01-01

The ferric uptake regulator (Fur) family proteins include sensors of Fe (Fur), Zn (Zur), and peroxide (PerR). Among Fur family proteins, Fur and Zur are ubiquitous in most prokaryotic organisms, whereas PerR exists mainly in Gram positive bacteria as a functional homologue of OxyR. Gram positive bacteria such as Bacillus subtilis, Listeria monocytogenes and Staphylococcus aureus encode three Fur family proteins: Fur, Zur, and PerR. In this study, we identified five Fur family proteins from B. licheniformis: two novel PerR-like proteins (BL00690 and BL00950) in addition to Fur (BL05249), Zur (BL03703), and PerR (BL00075) homologues. Our data indicate that all of the five B. licheniformis Fur homologues contain a structural Zn2+ site composed of four cysteine residues like many other Fur family proteins. Furthermore, we provide evidence that the PerR-like proteins (BL00690 and BL00950) as well as PerRBL (BL00075), but not FurBL (BL05249) and ZurBL (BL03703), can sense H2O2 by histidine oxidation with different sensitivity. We also show that PerR2 (BL00690) has a PerR-like repressor activity for PerR-regulated genes in vivo. Taken together, our results suggest that B. licheniformis contains three PerR subfamily proteins which can sense H2O2 by histidine oxidation not by cysteine oxidation, in addition to Fur and Zur. PMID:27176811

O2 availability impacts iron homeostasis in Escherichia coli.

PubMed

Beauchene, Nicole A; Mettert, Erin L; Moore, Laura J; Keleş, Sündüz; Willey, Emily R; Kiley, Patricia J

2017-11-14

The ferric-uptake regulator (Fur) is an Fe 2+ -responsive transcription factor that coordinates iron homeostasis in many bacteria. Recently, we reported that expression of the Escherichia coli Fur regulon is also impacted by O 2 tension. Here, we show that for most of the Fur regulon, Fur binding and transcriptional repression increase under anaerobic conditions, suggesting that Fur is controlled by O 2 availability. We found that the intracellular, labile Fe 2+ pool was higher under anaerobic conditions compared with aerobic conditions, suggesting that higher Fe 2+ availability drove the formation of more Fe 2+ -Fur and, accordingly, more DNA binding. O 2 regulation of Fur activity required the anaerobically induced FeoABC Fe 2+ uptake system, linking increased Fur activity to ferrous import under iron-sufficient conditions. The increased activity of Fur under anaerobic conditions led to a decrease in expression of ferric import systems. However, the combined positive regulation of the feoABC operon by ArcA and FNR partially antagonized Fur-mediated repression of feoABC under anaerobic conditions, allowing ferrous transport to increase even though Fur is more active. This design feature promotes a switch from ferric import to the more physiological relevant ferrous iron under anaerobic conditions. Taken together, we propose that the influence of O 2 availability on the levels of active Fur adds a previously undescribed layer of regulation in maintaining cellular iron homeostasis.

O2 availability impacts iron homeostasis in Escherichia coli

PubMed Central

Beauchene, Nicole A.; Mettert, Erin L.; Moore, Laura J.; Keleş, Sündüz; Willey, Emily R.; Kiley, Patricia J.

2017-01-01

The ferric-uptake regulator (Fur) is an Fe2+-responsive transcription factor that coordinates iron homeostasis in many bacteria. Recently, we reported that expression of the Escherichia coli Fur regulon is also impacted by O2 tension. Here, we show that for most of the Fur regulon, Fur binding and transcriptional repression increase under anaerobic conditions, suggesting that Fur is controlled by O2 availability. We found that the intracellular, labile Fe2+ pool was higher under anaerobic conditions compared with aerobic conditions, suggesting that higher Fe2+ availability drove the formation of more Fe2+-Fur and, accordingly, more DNA binding. O2 regulation of Fur activity required the anaerobically induced FeoABC Fe2+ uptake system, linking increased Fur activity to ferrous import under iron-sufficient conditions. The increased activity of Fur under anaerobic conditions led to a decrease in expression of ferric import systems. However, the combined positive regulation of the feoABC operon by ArcA and FNR partially antagonized Fur-mediated repression of feoABC under anaerobic conditions, allowing ferrous transport to increase even though Fur is more active. This design feature promotes a switch from ferric import to the more physiological relevant ferrous iron under anaerobic conditions. Taken together, we propose that the influence of O2 availability on the levels of active Fur adds a previously undescribed layer of regulation in maintaining cellular iron homeostasis. PMID:29087312

76 FR 45499 - Marine Mammals; Subsistence Taking of Northern Fur Seals; Harvest Estimates

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-29

.... 110718394-1392-01] RIN 0648-BB09 Marine Mammals; Subsistence Taking of Northern Fur Seals; Harvest Estimates... governing the subsistence taking of northern fur seals, this document summarizes the annual fur seal... annual estimates of fur seal subsistence needs for 2011 through 2013 on the Pribilof Islands, Alaska...

Cloning, overexpression and interaction of recombinant Fur from the cyanobacterium Anabaena PCC 7119 with isiB and its own promoter.

PubMed

Bes, M T; Hernández, J A; Peleato, M L; Fillat, M F

2001-01-15

A gene coding for a Fur (ferric uptake regulation) protein from the cyanobacterium Anabaena PCC 7119 has been cloned and overexpressed in Escherichia coli. DNA sequence analysis confirmed the presence of a 151-amino-acid open reading frame that showed homology with the Fur proteins reported for the unicellular cyanobacteria Synechococcus 7942 and Synechocystis PCC 6803. Two putative Fur-binding sites were detected in the promoter regions of the fur gene from Anabaena. Partially purified recombinant Fur binds to the flavodoxin promoter as well as its own promoter. This suggests that the Fur gene is autoregulated in Anabaena.

Microgravity Active Vibration Isolation System on Parabolic Flights

NASA Astrophysics Data System (ADS)

Dong, Wenbo; Pletser, Vladimir; Yang, Yang

2016-07-01

The Microgravity Active Vibration Isolation System (MAIS) aims at reducing on-orbit vibrations, providing a better controlled lower gravity environment for microgravity physical science experiments. The MAIS will be launched on Tianzhou-1, the first cargo ship of the China Manned Space Program. The principle of the MAIS is to suspend with electro-magnetic actuators a scientific payload, isolating it from the vibrating stator. The MAIS's vibration isolation capability is frequency-dependent and a decrease of vibration of about 40dB can be attained. The MAIS can accommodate 20kg of scientific payload or sample unit, and provide 30W of power and 1Mbps of data transmission. The MAIS is developed to support microgravity scientific experiments on manned platforms in low earth orbit, in order to meet the scientific requirements for fluid physics, materials science, and fundamental physics investigations, which usually need a very quiet environment, increasing their chances of success and their scientific outcomes. The results of scientific experiments and technology tests obtained with the MAIS will be used to improve future space based research. As the suspension force acting on the payload is very small, the MAIS can only be operative and tested in a weightless environment. The 'Deutsches Zentrum für Luft- und Raumfahrt e.V.' (DLR, German Aerospace Centre) granted a flight opportunity to the MAIS experiment to be tested during its 27th parabolic flight campaign of September 2015 performed on the A310 ZERO-G aircraft managed by the French company Novespace, a subsidiary of the 'Centre National d'Etudes Spatiales' (CNES, French Space Agency). The experiment results confirmed that the 6 degrees of freedom motion control technique was effective, and that the vibration isolation performance fulfilled perfectly the expectations based on theoretical analyses and simulations. This paper will present the design of the MAIS and the experiment results obtained during the parabolic flight campaign.

An airborne perfluorocarbon tracer system and its first application for a Lagrangian experiment

NASA Astrophysics Data System (ADS)

Ren, Y.; Baumann, R.; Schlager, H.

2015-01-01

A perfluorocarbon tracer system (PERTRAS), specifically designed for Lagrangian aircraft experiments, has been developed by the Deutsches Zentrum für Luft- und Raumfahrt (German Aerospace Center, DLR). It consists of three main parts: a tracer release unit (RU), an adsorption tube sampler (ATS), and a tracer analytical system. The RU was designed for airborne tracer release experiments; meanwhile, it can be used on various platforms for different experimental purposes (here research vessel). PERTRAS was for the first time applied in the field campaign Stratospheric ozone: Halogen Impacts in a Varying Atmosphere (SHIVA) in November 2011. An amount of 8.8 kg perfluoromethylcyclopentane (PMCP) was released aboard the research vessel Sonne (RV Sonne) near the operational site of this campaign, Miri, Malaysia, on 21 November. The tracer samples collected using the ATS onboard the DLR research aircraft Falcon were analyzed in the laboratory using a thermal desorber-gas chromatography-mass spectrometry (TD-GC-MS) system. Guided by forecasts calculated with the Lagrangian model Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLIT), 64 tracer samples were collected onboard the Falcon approximately 5 and 25 h after the release, mostly with a time resolution of 1 min. Enhanced PMCP concentrations relative to ambient PMCP background values (mean: 6.62 fmol mol-1) were detected during three intersects of the fresh tracer plume (age 5 h), with a maximum value of 301.33 fmol mol-1. This indicates that the fresh tracer plume was successfully intercepted at the forecast position. During the second flight, 25 h after the release, the center of tracer plume was not detected by the sampling system due to a faster advection of the plume than forecast. The newly developed PERTRAS system has been successfully deployed for the first time. The instrumental setup and comparisons between the measurements and HYSPLIT simulations are presented in this study.

An airborne perfluorocarbon tracer system and its first application for a Lagrangian experiment

NASA Astrophysics Data System (ADS)

Ren, Y.; Baumann, R.; Schlager, H.

2014-07-01

A perfluorocarbon tracer system (PERTRAS), specifically designed for Lagrangian aircraft experiments, has been developed by the Deutsches Zentrum für Luft- und Raumfahrt (German Aerospace Center, DLR). It consists of three main parts: a tracer release unit (RU), an adsorption tube sampler (ATS) and a tracer analytical system. The RU was designed for airborne tracer release experiments; meanwhile, it can be used on various platforms for different experimental purpose (here research vessel). PERTRAS was for the first time applied in the field campaign Stratospheric ozone: halogen Impacts in a Varying Atmosphere (SHIVA) in November 2011. An amount of 8.8 kg perfluoromethylcyclopentane (PMCP) was released aboard the research vessel Sonne (RV Sonne) near the operational site of this campaign, Miri, Malaysia, on 21 November. The tracer samples collected using the ATS on board the DLR research aircraft Falcon were analyzed in the laboratory using a thermal desorber/gas chromatography/mass spectrometry (TD/GC/MS) system. Guided by forecasts calculated with the Lagrangian model, Hybrid Single Particle Lagrangian Integrated Trajectory (HYSPLIT), 64 tracer samples were collected onboard the Falcon approximately 5 and 25 h after the release, respectively, mostly with a time resolution of 1 min. Enhanced PMCP concentrations relative to ambient PMCP background values (mean: 6.62 fmol mol-1) were detected during three intersects of the fresh tracer plume (age 5 h), with a maximum value of 301.33 fmol mol-1. This indicates that the fresh tracer plume was successfully intercepted at the forecasted position. During the second flight, 25 h after the release, the center of tracer plume was not detected by the sampling system due to a faster advection of the plume than forecasted. The newly developed PERTRAS system has been successfully deployed for the first time. The instrumental set-up and comparisons between the measurements and HYSPLIT simulations are presented in this study.

Deriving an atmospheric budget of total organic bromine using airborne in-situ measurements from the Western Pacific during SHIVA

NASA Astrophysics Data System (ADS)

Sala, S.; Bönisch, H.; Keber, T.; Oram, D. E.; Mills, G.; Engel, A.

2014-02-01

During the SHIVA (Stratospheric Ozone: Halogen Impacts in a Varying Atmosphere) project an extensive dataset of all halogen species relevant for the atmospheric budget of total organic bromine has been collected in the West Pacific region using the FALCON aircraft operated by the German Aerospace agency DLR (Deutsches Zentrum für Luft- und Raumfahrt) covering a vertical range from the planetary boundary layer up to the ceiling altitude of the aircraft of 13 km. In total, more than 700 measurements were performed with the newly developed fully-automated in-situ instrument GHOST-MS (Gas cHromatograph for the Observation of Tracers - coupled with a Mass Spectrometer) by the Goethe University of Frankfurt (GUF) and with the onboard whole-air sampler WASP with subsequent ground based state-of-the-art GC/MS analysis by the University of East Anglia (UEA). Both instruments yield good agreement for all major (CHBr3 and CH2Br2) and minor (CHBrCl, CHBrCl2 and CHBr2Cl) VSLS (very short-lived substances), at least at the level of their 2 σ measurement uncertainties. In contrast to the suggestion that the Western Pacific could be a major source region for VSLS (Pyle et al., 2011), we found only slightly enhanced mixing ratios of brominated halogen source gases relative to the levels reported in Montzka et al. (2011) for other tropical regions. A budget for total organic bromine, including all four halons,CH3Br and the VSLS, is derived for the upper troposphere, the input region for the TTL and thus also for the stratosphere, compiled from the SHIVA dataset. With exception of the two minor VSLS CHBrCl2 and CHBr2Cl, excellent agreement with the values reported in Montzka et al. (2011) is found, while being slightly higher than previous studies from our group based on balloon-borne measurements.

19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 19 Customs Duties 1 2011-04-01 2011-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or other...

19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 19 Customs Duties 1 2010-04-01 2010-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or other...

19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 19 Customs Duties 1 2013-04-01 2013-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or other...

19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 19 Customs Duties 1 2012-04-01 2012-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or other...

19 CFR 12.61 - Fur-seal or sea-otter skins permitted entry.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 19 Customs Duties 1 2014-04-01 2014-04-01 false Fur-seal or sea-otter skins permitted entry. 12.61...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.61 Fur-seal or sea-otter skins permitted entry. (a) Fur-seal or sea-otter skins taken by Indians, Aleuts, or other...

Relationship of the superoxide dismutase genes, sodA and sodB, to the iron uptake (/ital fur/) regulon in /ital Escherichia coli/ K-12

DOE Office of Scientific and Technical Information (OSTI.GOV)

Niederhoffer, E.C.; Naranjo, C.M.; Fee, J.A.

1988-01-01

Expression of sodA, as indicated by MnSod activity is normal in /ital fur/ mutants. This suggests that sodA is not a member of the /ital fur/ regulon and that the putative Fe-binding, regulatory protein of sodA, suggested by Moody and Hassan is not the Fur protein. by contrast, expression of sodB, as indicated by FeSod activity, is completely blocked in /ital fur/ mutants and the effect is restored by transformation with a plasmid having a normal /ital fur/ locus. The observations suggest that Fur, either directly or indirectly, controls SodB biosynthesis. Additional observations are described which indicate that SodB andmore » Fur act together in a complicated fashion to control the biosynthesis of enterobactin. 26 refs., 3 tabs.« less

50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

Code of Federal Regulations, 2014 CFR

2014-10-01

... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear harvested in... trade internationally in fur skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf... lynx (Lynx canadensis), gray wolf (Canis lupus), and brown bear (Ursus arctos) harvested in the United...

Control of Fur synthesis by the non-coding RNA RyhB and iron-responsive decoding.

PubMed

Vecerek, Branislav; Moll, Isabella; Bläsi, Udo

2007-02-21

The Fe2+-dependent Fur protein serves as a negative regulator of iron uptake in bacteria. As only metallo-Fur acts as an autogeneous repressor, Fe2+scarcity would direct fur expression when continued supply is not obviously required. We show that in Escherichia coli post-transcriptional regulatory mechanisms ensure that Fur synthesis remains steady in iron limitation. Our studies revealed that fur translation is coupled to that of an upstream open reading frame (uof), translation of which is downregulated by the non-coding RNA (ncRNA) RyhB. As RyhB transcription is negatively controlled by metallo-Fur, iron depletion creates a negative feedback loop. RyhB-mediated regulation of uof-fur provides the first example for indirect translational regulation by a trans-encoded ncRNA. In addition, we present evidence for an iron-responsive decoding mechanism of the uof-fur entity. It could serve as a backup mechanism of the RyhB circuitry, and represents the first link between iron availability and synthesis of an iron-containing protein.

Inactivation of ferric uptake regulator (Fur) attenuates Helicobacter pylori J99 motility by disturbing the flagellar motor switch and autoinducer-2 production.

PubMed

Lee, Ai-Yun; Kao, Cheng-Yen; Wang, Yao-Kuan; Lin, Ssu-Yuan; Lai, Tze-Ying; Sheu, Bor-Shyang; Lo, Chien-Jung; Wu, Jiunn-Jong

2017-08-01

Flagellar motility of Helicobacter pylori has been shown to be important for the bacteria to establish initial colonization. The ferric uptake regulator (Fur) is a global regulator that has been identified in H. pylori which is involved in the processes of iron uptake and establishing colonization. However, the role of Fur in H. pylori motility is still unclear. Motility of the wild-type, fur mutant, and fur revertant J99 were determined by a soft-agar motility assay and direct video observation. The bacterial shape and flagellar structure were evaluated by transmission electron microscopy. Single bacterial motility and flagellar switching were observed by phase-contrast microscopy. Autoinducer-2 (AI-2) production in bacterial culture supernatant was analyzed by a bioluminescence assay. The fur mutant showed impaired motility in the soft-agar assay compared with the wild-type J99 and fur revertant. The numbers and lengths of flagellar filaments on the fur mutant cells were similar to those of the wild-type and revertant cells. Phenotypic characterization showed similar swimming speed but reduction in switching rate in the fur mutant. The AI-2 production of the fur mutant was dramatically reduced compared with wild-type J99 in log-phase culture medium. These results indicate that Fur positively modulates H. pylori J99 motility through interfering with bacterial flagellar switching. © 2017 John Wiley & Sons Ltd.

Coping with heat: function of the natal coat of cape fur seal (Arctocephalus Pusillus Pusillus) pups in maintaining core body temperature.

PubMed

Erdsack, Nicola; Dehnhardt, Guido; Hanke, Wolf

2013-01-01

Cape fur seal (Arctocephalus pusillus) pups spend the first weeks of life exclusively or mainly ashore. They are exposed to intense solar radiation and high temperatures for long time periods, which results in temperatures up to at least 80°C on their black natal coat. To test the hypothesis that the natal coat has a crucial function in coping with these extreme conditions, we investigated the insulating properties of the natal coat in six captive newborn Cape fur seals during the first 50 days after birth. The natal fur differs from the adult fur not only in colour, but also in density, structure, and water repellence. We measured temperature on the fur surface and within the fur, as well as skin and rectal temperature under varying environmental conditions, comparable to the species' habitat. Experiments were designed to not influence the spontaneous behaviour of the pups. Rectal temperature was constant as long as the pups stayed dry, even during long-lasting intense solar radiation for up to 3 h. Skin temperature remained close to rectal temperature as long as the fur was dry, while with wet fur, skin temperature was significantly reduced as well. Our results show that the natal coat provides an effective insulation against overheating. The severely reduced insulation of wet natal fur against cold supports the assumption that the natal fur is an adaptation to the pups' terrestrial phase of life.

Coping with Heat: Function of The Natal Coat of Cape Fur Seal (Arctocephalus Pusillus Pusillus) Pups in Maintaining Core Body Temperature

PubMed Central

Erdsack, Nicola; Dehnhardt, Guido; Hanke, Wolf

2013-01-01

Cape fur seal (Arctocephalus pusillus) pups spend the first weeks of life exclusively or mainly ashore. They are exposed to intense solar radiation and high temperatures for long time periods, which results in temperatures up to at least 80°C on their black natal coat. To test the hypothesis that the natal coat has a crucial function in coping with these extreme conditions, we investigated the insulating properties of the natal coat in six captive newborn Cape fur seals during the first 50 days after birth. The natal fur differs from the adult fur not only in colour, but also in density, structure, and water repellence. We measured temperature on the fur surface and within the fur, as well as skin and rectal temperature under varying environmental conditions, comparable to the species' habitat. Experiments were designed to not influence the spontaneous behaviour of the pups. Rectal temperature was constant as long as the pups stayed dry, even during long-lasting intense solar radiation for up to 3 h. Skin temperature remained close to rectal temperature as long as the fur was dry, while with wet fur, skin temperature was significantly reduced as well. Our results show that the natal coat provides an effective insulation against overheating. The severely reduced insulation of wet natal fur against cold supports the assumption that the natal fur is an adaptation to the pups' terrestrial phase of life. PMID:23951287

Generic names of northern and southern fur seals (Mammalia: Otariidae)

USGS Publications Warehouse

Gardner, A.L.; Robbins, C.B.

1998-01-01

We have resolved a nomenclatural problem discovered during research on the northern fur seal that concerns the correct generic name for this taxon and for fur seals of the Southern Hemisphere. The unfortunate practice by some 19th century authors to use names in their Latinized form, but to date them from their first appearance as French common names led to the use of Arctocephalus for southern fur seals when the name correctly applies to the northern fur seal, known today as Callorhinus ursinus. However, Arctocephalus and Callorhinus are antedated by Otoes G. Fischer, 1817, which is the earliest available generic for the fur seal of the northern Pacific. The earliest available generic name for southern fur seals is Halarctus Gill, 1866. To avoid the confusion that would result from replacing the currently used generic names with those required by strict adherence to the Principle of Priority, we have petitioned the International Commission on Zoological nomenclature to preserve Arctocephalus and Callorhinus for the southern and northern fur seals, respectively.

16 CFR 301.22 - Disclosure of damaged furs.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Disclosure of damaged furs. 301.22 Section 301.22 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES... decrease the normal life and durability of such product. (b) When damaged furs are used in a fur product...

50 CFR 216.71 - Allowable take of fur seals.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Allowable take of fur seals. 216.71... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.71 Allowable take of fur seals. Pribilovians may take fur seals on the Pribilof Islands if such taking is (a) For subsistence uses, and (b) Not...

50 CFR 216.73 - Disposition of fur seal parts.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Disposition of fur seal parts. 216.73... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.73 Disposition of fur seal parts. Except... part of a fur seal taken for subsistence uses may be sold or otherwise transferred to any person unless...

50 CFR 216.71 - Allowable take of fur seals.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Allowable take of fur seals. 216.71... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.71 Allowable take of fur seals. Pribilovians may take fur seals on the Pribilof Islands if such taking is (a) For subsistence uses, and (b) Not...

50 CFR 216.81 - Visits to fur seal rookeries.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Visits to fur seal rookeries. 216.81... MAMMALS Pribilof Islands Administration § 216.81 Visits to fur seal rookeries. From June 1 to October 15... any fur seal rookery or hauling grounds nor pass beyond any posted sign forbidding passage. [41 FR...

50 CFR 216.81 - Visits to fur seal rookeries.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Visits to fur seal rookeries. 216.81... MAMMALS Pribilof Islands Administration § 216.81 Visits to fur seal rookeries. From June 1 to October 15... any fur seal rookery or hauling grounds nor pass beyond any posted sign forbidding passage. [41 FR...

50 CFR 216.73 - Disposition of fur seal parts.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Disposition of fur seal parts. 216.73... MAMMALS Pribilof Islands, Taking for Subsistence Purposes § 216.73 Disposition of fur seal parts. Except... part of a fur seal taken for subsistence uses may be sold or otherwise transferred to any person unless...

Normal hematology and serum chemistry of northern fur seals (Callorhinus ursinus) in captivity.

PubMed

Kohyama, Kaoru; Inoshima, Yasuo

2017-09-01

Northern fur seals (Callorhinus ursinus) are endemic to the North Pacific Ocean. They were hunted for their fur and became endangered in the late 1800s, but their populations recovered following the introduction of protection laws. Recently, populations have been decreasing again, although the reasons are unclear. For individuals that are bred and reared in captivity as part of ex situ conservation projects, details of blood characteristics are essential to ensure good health. However, the normal ranges of hematology and serum chemistry of captive northern fur seals have not been defined. This study determined the normal ranges of hematology and serum chemistry of captive fur seals. Blood samples were collected every month for 2 years from four captive northern fur seals in Japan (three born in an aquarium and one kept in the same aquarium following rescue). Fifteen blood characteristics and 29 serum chemistry properties were compared with those previously reported for wild northern fur seals in the USA. Several parameters were not within the normal ranges reported previously in wild northern fur seals. In particular, levels of alkaline phosphatase was outside of the normal ranges previously reported. The hematological and serum chemistry ranges in this study can help provide a guideline for understanding the health of northern fur seals in captivity. © 2017 Wiley Periodicals, Inc.

Helicobacter pylori Urease Activity is Influenced by Ferric Uptake Regulator

PubMed Central

Lee, Jong Seung; Lee, Ji Hyuk; Lee, Hye Jin; Lee, Jee Hyun; Choi, Young Ok

2010-01-01

Purpose The role of the Ferric Uptake Regulator (FUR) in the acid resistance of Helicobacter pylori (H. pylori) has been thought to be independent of urease. However, we demonstrated in this study that Fur influences urease activity. Materials and Methods A fur knockout mutant of H. pylori was constructed by replacing the Fur gene with a kanamycin resistant marker gene. The wild-type H. pylori and fur mutant were compared for survival. The integrity of the inner membrane of the bacteria was evaluated by confocal microscopy using membrane-permeant and -impermeant fluorescent DNA probes. Urease activity of intact H. pylori was measured between pH 3 and 8. Real time PCR of both strains was performed for urease genes including ureI, ureE, ureF, ureG, and ureH. Results The fur deletion affected the survival of H. pylori at pH 4. The urease activity curve of the intact fur mutant showed the same shape as the wild-type but was 3-fold lower than the wild-type at a pH of less than 5. Real time PCR revealed that the expression of all genes was consistently down-regulated in the fur mutant. Conclusion The results of this study showed that fur appears to be involved in acid resistant H. pylori urease activity. PMID:20046512

Transcriptional regulation by Ferric Uptake Regulator (Fur) in pathogenic bacteria.

PubMed

Troxell, Bryan; Hassan, Hosni M

2013-01-01

In the ancient anaerobic environment, ferrous iron (Fe(2+)) was one of the first metal cofactors. Oxygenation of the ancient world challenged bacteria to acquire the insoluble ferric iron (Fe(3+)) and later to defend against reactive oxygen species (ROS) generated by the Fenton chemistry. To acquire Fe(3+), bacteria produce low-molecular weight compounds, known as siderophores, which have extremely high affinity for Fe(3+). However, during infection the host restricts iron from pathogens by producing iron- and siderophore-chelating proteins, by exporting iron from intracellular pathogen-containing compartments, and by limiting absorption of dietary iron. Ferric Uptake Regulator (Fur) is a transcription factor which utilizes Fe(2+) as a corepressor and represses siderophore synthesis in pathogens. Fur, directly or indirectly, controls expression of enzymes that protect against ROS damage. Thus, the challenges of iron homeostasis and defense against ROS are addressed via Fur. Although the role of Fur as a repressor is well-documented, emerging evidence demonstrates that Fur can function as an activator. Fur activation can occur through three distinct mechanisms (1) indirectly via small RNAs, (2) binding at cis regulatory elements that enhance recruitment of the RNA polymerase holoenzyme (RNAP), and (3) functioning as an antirepressor by removing or blocking DNA binding of a repressor of transcription. In addition, Fur homologs control defense against peroxide stress (PerR) and control uptake of other metals such as zinc (Zur) and manganese (Mur) in pathogenic bacteria. Fur family members are important for virulence within bacterial pathogens since mutants of fur, perR, or zur exhibit reduced virulence within numerous animal and plant models of infection. This review focuses on the breadth of Fur regulation in pathogenic bacteria.

Transcriptional regulation by Ferric Uptake Regulator (Fur) in pathogenic bacteria

PubMed Central

Troxell, Bryan; Hassan, Hosni M.

2013-01-01

In the ancient anaerobic environment, ferrous iron (Fe2+) was one of the first metal cofactors. Oxygenation of the ancient world challenged bacteria to acquire the insoluble ferric iron (Fe3+) and later to defend against reactive oxygen species (ROS) generated by the Fenton chemistry. To acquire Fe3+, bacteria produce low-molecular weight compounds, known as siderophores, which have extremely high affinity for Fe3+. However, during infection the host restricts iron from pathogens by producing iron- and siderophore-chelating proteins, by exporting iron from intracellular pathogen-containing compartments, and by limiting absorption of dietary iron. Ferric Uptake Regulator (Fur) is a transcription factor which utilizes Fe2+ as a corepressor and represses siderophore synthesis in pathogens. Fur, directly or indirectly, controls expression of enzymes that protect against ROS damage. Thus, the challenges of iron homeostasis and defense against ROS are addressed via Fur. Although the role of Fur as a repressor is well-documented, emerging evidence demonstrates that Fur can function as an activator. Fur activation can occur through three distinct mechanisms (1) indirectly via small RNAs, (2) binding at cis regulatory elements that enhance recruitment of the RNA polymerase holoenzyme (RNAP), and (3) functioning as an antirepressor by removing or blocking DNA binding of a repressor of transcription. In addition, Fur homologs control defense against peroxide stress (PerR) and control uptake of other metals such as zinc (Zur) and manganese (Mur) in pathogenic bacteria. Fur family members are important for virulence within bacterial pathogens since mutants of fur, perR, or zur exhibit reduced virulence within numerous animal and plant models of infection. This review focuses on the breadth of Fur regulation in pathogenic bacteria. PMID:24106689

77 FR 41168 - Marine Mammals; Subsistence Taking of Northern Fur Seals; St. Paul Island

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-12

... Mammals; Subsistence Taking of Northern Fur Seals; St. Paul Island AGENCY: National Marine Fisheries... taking of northern fur seals on St. Paul Island. St. Paul's petition requests that NMFS revise the... seals; take a total of up to 3,000 fur seals annually compared to 2,000 currently allowed, including up...

Fur productivity of submarginal farmland

USGS Publications Warehouse

Uhler, F.M.; Llewellyn, L.M.

1952-01-01

A submarginal tract of a thousand acres on the Patuxent Research Refuge, Laurel, Maryland, was trapped for three seasons (1943-46) to determine the fur-productivity of the area. The tract yielded 392 fur animals, the pelts of which were sold at public auction for $1119.00. This resulted in an average income from trapping of approximately forty cents per acre per year. The habitats most productive of catches of fur animals were hedgerows and wood and road margins, followed by bottomland forests and lake margins. Some suggestions for improving habitat for fur animals are given.

Pet fur color and texture classification

NASA Astrophysics Data System (ADS)

Yen, Jonathan; Mukherjee, Debarghar; Lim, SukHwan; Tretter, Daniel

2007-01-01

Object segmentation is important in image analysis for imaging tasks such as image rendering and image retrieval. Pet owners have been known to be quite vocal about how important it is to render their pets perfectly. We present here an algorithm for pet (mammal) fur color classification and an algorithm for pet (animal) fur texture classification. Per fur color classification can be applied as a necessary condition for identifying the regions in an image that may contain pets much like the skin tone classification for human flesh detection. As a result of the evolution, fur coloration of all mammals is caused by a natural organic pigment called Melanin and Melanin has only very limited color ranges. We have conducted a statistical analysis and concluded that mammal fur colors can be only in levels of gray or in two colors after the proper color quantization. This pet fur color classification algorithm has been applied for peteye detection. We also present here an algorithm for animal fur texture classification using the recently developed multi-resolution directional sub-band Contourlet transform. The experimental results are very promising as these transforms can identify regions of an image that may contain fur of mammals, scale of reptiles and feather of birds, etc. Combining the color and texture classification, one can have a set of strong classifiers for identifying possible animals in an image.

16 CFR 300.8 - Use of fiber trademark and generic names.

Code of Federal Regulations, 2011 CFR

2011-01-01

... is not the case. (g) The term fur fiber may be used to describe the hair or fur fiber or mixtures..., llama and vicuna. If the name, symbol, or depiction of any animal producing the hair or fur fiber is..., the percentage by weight of such hair or fur fiber in the total fiber weight of the wool product shall...

16 CFR 300.8 - Use of fiber trademark and generic names.

Code of Federal Regulations, 2010 CFR

2010-01-01

... is not the case. (g) The term fur fiber may be used to describe the hair or fur fiber or mixtures..., llama and vicuna. If the name, symbol, or depiction of any animal producing the hair or fur fiber is..., the percentage by weight of such hair or fur fiber in the total fiber weight of the wool product shall...

16 CFR 300.8 - Use of fiber trademark and generic names.

Code of Federal Regulations, 2012 CFR

2012-01-01

... is not the case. (g) The term fur fiber may be used to describe the hair or fur fiber or mixtures..., llama and vicuna. If the name, symbol, or depiction of any animal producing the hair or fur fiber is..., the percentage by weight of such hair or fur fiber in the total fiber weight of the wool product shall...

16 CFR 300.8 - Use of fiber trademark and generic names.

Code of Federal Regulations, 2014 CFR

2014-01-01

... is not the case. (g) The term fur fiber may be used to describe the hair or fur fiber or mixtures..., llama and vicuna. If the name, symbol, or depiction of any animal producing the hair or fur fiber is..., the percentage by weight of such hair or fur fiber in the total fiber weight of the wool product shall...

16 CFR 300.8 - Use of fiber trademark and generic names.

Code of Federal Regulations, 2013 CFR

2013-01-01

... is not the case. (g) The term fur fiber may be used to describe the hair or fur fiber or mixtures..., llama and vicuna. If the name, symbol, or depiction of any animal producing the hair or fur fiber is..., the percentage by weight of such hair or fur fiber in the total fiber weight of the wool product shall...

19 CFR 11.12a - Labeling of fur products to indicate composition.

Code of Federal Regulations, 2010 CFR

2010-04-01

... violation of the act with respect to imported articles comes to the attention of a port director, the... “fur product” means any article of wearing apparel made in whole or in part of fur or used fur; except that such term shall not include such articles as the Federal Trade Commission shall exempt by reason...

Web-Based Requesting and Scheduling Use of Facilities

NASA Technical Reports Server (NTRS)

Yeager, Carolyn M.

2010-01-01

Automated User's Training Operations Facility Utilization Request (AutoFUR) is prototype software that administers a Web-based system for requesting and allocating facilities and equipment for astronaut-training classes in conjunction with scheduling the classes. AutoFUR also has potential for similar use in such applications as scheduling flight-simulation equipment and instructors in commercial airplane-pilot training, managing preventive- maintenance facilities, and scheduling operating rooms, doctors, nurses, and medical equipment for surgery. Whereas requesting and allocation of facilities was previously a manual process that entailed examination of documents (including paper drawings) from different sources, AutoFUR partly automates the process and makes all of the relevant information available via the requester s computer. By use of AutoFUR, an instructor can fill out a facility-utilization request (FUR) form on line, consult the applicable flight manifest(s) to determine what equipment is needed and where it should be placed in the training facility, reserve the corresponding hardware listed in a training-hardware inventory database, search for alternative hardware if necessary, submit the FUR for processing, and cause paper forms to be printed. Auto-FUR also maintains a searchable archive of prior FURs.

50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

Code of Federal Regulations, 2012 CFR

2012-10-01

... WILDLIFE AND PLANTS (CONTINUED) CONVENTION ON INTERNATIONAL TRADE IN ENDANGERED SPECIES OF WILD FAUNA AND FLORA (CITES) International Trade in Certain Specimens § 23.69 How can I trade internationally in fur... 50 Wildlife and Fisheries 9 2012-10-01 2012-10-01 false How can I trade internationally in fur...

50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

Code of Federal Regulations, 2010 CFR

2010-10-01

... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? 23.69... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? (a) U.S. and...), river otter (Lontra canadensis), and Canada lynx (Lynx canadensis), and the Alaskan populations of gray...

50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

Code of Federal Regulations, 2011 CFR

2011-10-01

... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? 23.69... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? (a) U.S. and...), river otter (Lontra canadensis), and Canada lynx (Lynx canadensis), and the Alaskan populations of gray...

50 CFR 23.69 - How can I trade internationally in fur skins and fur skin products of bobcat, river otter, Canada...

Code of Federal Regulations, 2013 CFR

2013-10-01

... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? 23.69... skins and fur skin products of bobcat, river otter, Canada lynx, gray wolf, and brown bear? (a) U.S. and...), river otter (Lontra canadensis), and Canada lynx (Lynx canadensis), and the Alaskan populations of gray...

Comparison of flexible ureterorenoscopy and mini-percutaneous nephrolithotomy in treatment of lower calyceal stones smaller than 2 cm.

PubMed

Akbulut, Fatih; Kucuktopcu, Onur; Kandemir, Emre; Sonmezay, Erkan; Simsek, Abdulmuttalip; Ozgor, Faruk; Binbay, Murat; Muslumanoglu, Ahmet Yaser; Gurbuz, Gokhan

2016-01-01

To compare the outcomes of flexible ureterorenoscopy (F-URS) and mini-percutaneous nephrolithotomy (mini-PNL) in the treatment of lower calyceal stones smaller than 2 cm. Patients who underwent F-URS and mini-PNL for the treatment of lower calyceal stones smaller than 2 cm between March 2009 and December 2014 were retrospectively evaluated. Ninety-four patients were divided into two groups by treatment modality: F-URS (Group 1: 63 patients) and mini-PNL (Group 2: 31 patients). All patients were preoperatively diagnosed with intravenous pyelography or computed tomography. Success rates for F-URS and mini-PNL at postoperative first month were 85.7% and 90.3%, respectively. Operation time, fluoroscopy time, and hospitalization time for F-URS and mini-PNL patients were 44.40 min, 2.9 min, 22.4 h, and 91.9 min, 6.4 min, and 63.8 h, respectively. All three parameters were significantly shorter among the F-URS group (p < 0.001). Postoperative hemoglobin drop was significantly lower in F-URS group compared to mini-PNL group (0.39 mg/dL vs. 1.15 mg/dL, p = 0.001). A comparison of complications according to the Clavien classification demonstrated significant differences between the groups (p = 0.001). More patients in the F-URS groups require antibiotics due to urinary tract infection, and more patients in the mini-PNL group required ureteral double J catheter insertion under general anesthesia. Although both F-URS and mini-PNL have similar success rates for the treatment of lower calyceal stones, F-URS appears to be more favorable due to shorter fluoroscopy and hospitalization times; and lower hemoglobin drops. Multicenter and studies using higher patient volumes are needed to confirm these findings.

Overexpression of FurA in Anabaena sp. PCC 7120 reveals new targets for this regulator involved in photosynthesis, iron uptake and cellular morphology.

PubMed

González, Andrés; Bes, M Teresa; Barja, François; Peleato, M Luisa; Fillat, María F

2010-11-01

Previous genomic analyses of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 have identified three ferric uptake regulator (Fur) homologs with low sequence identities and probably different functions in the cell. FurA is a constitutive protein that shares the highest homology with Fur from heterotrophic bacteria and appears to be essential for in vitro growth. In this study, we have analysed the effects of FurA overexpression on the Anabaena sp. phenotype and investigated which of the observed alterations were directly operated by FurA. Overexpression of the regulator led to changes in cellular morphology, resulting in shorter filaments with rounded cells of different sizes. The furA-overexpressing strain showed a slower photoautotrophic growth and a marked decrease in the oxygen evolution rate. Overexpression of the regulator also decreased both catalase and superoxide dismutase activities, but did not lead to an increase in the levels of intracellular reactive oxygen species. By combining phenotypic studies, reverse transcription-PCR analyses and electrophoretic mobility shift assays, we identified three novel direct targets of FurA, including genes encoding a siderophore outer membrane transporter (schT), bacterial actins (mreBCD) and the PSII reaction center protein D1 (psbA). The affinity of FurA for these novel targets was markedly affected by the absence of divalent metal ions, confirming previous evidence of a critical role for the metal co-repressor in the function of the regulator in vivo. The results unravel new cellular processes modulated by FurA, supporting its role as a global transcriptional regulator in Anabaena sp. PCC 7120.

Efficacy Management of Urolithiasis: Flexible Ureteroscopy versus Extracorporeal Shockwave Lithotripsy.

PubMed

Tauber, Volkmar; Wohlmuth, Martin; Hochmuth, Andreas; Schimetta, Wolfgang; Schimetta, Wofgang; Krause, F Steffen

2015-01-01

To evaluate the efficacy of flexible ureterscopy (fURS) and extracorporal shockwave lithotripsy (SWL) in the treatment of urolithiasis, complemented by a subgroup analysis of lower pole calyx. Retrospective analysis of patients treated by fURS or SWL was performed by independent variables such as gender, age, nephrolith size, double-J stent (DJ stent) and stone localisation. Out of 326 patients, 165 were treated by SWL and 161 by fURS. Complete stone removal was achieved by fURS in 83.2% and by SWL in 43.0% (p < 0.001). Asymptomatic behaviour (88-89%) and complication rate (10-11%) were nearly the same in both methods. A higher retreatment rate for SWL was necessary; otherwise, an auxillary DJ stent was performed more often preoperative before fURS. The subgroup analysis of lower pole calyx confirmed these evaluations. Complete stone-free removal was almost 8 times higher after fURS compared to SWL. The efficacy of fURS in treatment of urolithiasis is substantially higher than the efficacy of SWL. © 2015 S. Karger AG, Basel.

Uncinariasis in northern fur seal and California sea lion pups from California.

PubMed

Lyons, E T; DeLong, R L; Melin, S R; Tolliver, S C

1997-10-01

Northern fur seal (Callorhinus ursinus) (n = 25) and California sea lion (Zalophus californianus) (n = 53) pups, found dead on rookeries on San Miguel Island (California, USA), were examined for adult Uncinaria spp. Prevalence of these nematodes was 96% in fur seal pups and 100% in sea lion pups. Mean intensity of Uncinaria spp. per infected pup was 643 in fur seals and 1,284 in sea lions. Eggs of Uncinaria spp. from dead sea lion pups underwent embryonation in an incubator; development to the free-living third stage larva occurred within the egg. This study provided some specific information on hookworm infections in northern fur seal and California sea lion pups on San Miguel Island. High prevalence rate of Uncinaria spp. in both species of pinnipeds was documented and much higher numbers (2X) of hookworms were present in sea lion than fur seal pups.

Architecture of a Fur Binding Site: a Comparative Analysis

PubMed Central

Lavrrar, Jennifer L.; McIntosh, Mark A.

2003-01-01

Fur is an iron-binding transcriptional repressor that recognizes a 19-bp consensus site of the sequence 5′-GATAATGATAATCATTATC-3′. This site can be defined as three adjacent hexamers of the sequence 5′-GATAAT-3′, with the third being slightly imperfect (an F-F-F configuration), or as two hexamers in the forward orientation separated by one base pair from a third hexamer in the reverse orientation (an F-F-x-R configuration). Although Fur can bind synthetic DNA sequences containing the F-F-F arrangement, most natural binding sites are variations of the F-F-x-R arrangement. The studies presented here compared the ability of Fur to recognize synthetic DNA sequences containing two to four adjacent hexamers with binding to sequences containing variations of the F-F-x-R arrangement (including natural operator sequences from the entS and fepB promoter regions of Escherichia coli). Gel retardation assays showed that the F-F-x-R architecture was necessary for high-affinity Fur-DNA interactions and that contiguous hexamers were not recognized as effectively. In addition, the stoichiometry of Fur at each binding site was determined, showing that Fur interacted with its minimal 19-bp binding site as two overlapping dimers. These data confirm the proposed overlapping-dimer binding model, where the unit of interaction with a single Fur dimer is two inverted hexamers separated by a C:G base pair, with two overlapping units comprising the 19-bp consensus binding site required for the high-affinity interaction with two Fur dimers. PMID:12644489

16 CFR 301.32 - Fur product containing material other than fur.

Code of Federal Regulations, 2010 CFR

2010-01-01

... required under this Act; as for example: 100% Wool Interlining—100% Recycled Wool Trim—Dyed Muskrat Fur... required under the Act and rules and regulations; as for example: Body—Leather Trim—Dyed Mink [26 FR 3187...

16 CFR 301.32 - Fur product containing material other than fur.

Code of Federal Regulations, 2011 CFR

2011-01-01

... required under this Act; as for example: 100% Wool Interlining—100% Recycled Wool Trim—Dyed Muskrat Fur... required under the Act and rules and regulations; as for example: Body—Leather Trim—Dyed Mink [26 FR 3187...

The Bonn Astro/Geo Correlator

NASA Technical Reports Server (NTRS)

Bernhart, Simone; Alef, Walter; Bertarini, Alessandra; La Porta, Laura; Muskens, Arno; Rottmann, Helge; Roy, Alan

2013-01-01

The Bonn Distributed FX (DiFX) correlator is a software correlator operated jointly by the Max- Planck-Institut fur Radioastronomie (MPIfR), the Institut fur Geodasie und Geoinformation der Universitat Bonn (IGG), and the Bundesamt fur Kartographie und Geodasie (BKG) in Frankfurt.

Apo and Iron Bound Fur Repression and the Role of Fur in vivo

DTIC Science & Technology

2010-03-24

follicles subsequently provide the platform for development of mucosa- 20 associated lymphoid tissue (MALT) in which gastric marginal zone lymphoma can...pylori is able to effectively colonize this niche. One of the tools that H. pylori utilizes to respond to the acidic environment is urease, which is...manner than that of other organisms. H. pylori Fur appears unique in that it also utilizes apo-Fur regulation (18, 66). In this form of regulation

Characterization of the faecal bacterial community of wild young South American (Arctocephalus australis) and Subantarctic fur seals (Arctocephalus tropicalis).

PubMed

Medeiros, Aline Weber; Giongo, Adriana; Valdez, Fernanda P; Blaese de Amorin, Derek; Tavares, Maurício; d'Azevedo, Pedro A; Franco, Ana Claudia; Frazzon, Jeverson; Frazzon, Ana P G

2016-03-01

The microbiota of wild marine mammals is poorly understood, perhaps due to the migratory habits of some species and the difficulty in obtaining samples. Using high-throughput sequencing, the present study examines the faecal bacterial community of wild young South American (Arctocephalus australis) and Subantarctic fur seals (A. tropicalis). Faecal samples from South American (n = 6) and Subantarctic fur seals (n = 4) found dead along the south coast of Brazil were collected. Sequences were assigned to taxa using the Ribosomal Database Project-Bayesian classifier. Diversity of the microbiota was assessed by categorization of sequence reads into operational taxonomic units. Results indicate that Firmicutes (88.556%-84.016%) was the predominant phylum in South American and Subantarctic fur seals. The distribution of Actinobacteria and Proteobacteria varied according to the fur seal species. Fusobacteria and Bacteroidetes represented less than 1% of the sequences. The most abundant order in both fur seals was Clostridiales (88.64% and 87.49%). Individual variable incidences were observed in the composition of family among the fur seals, though the families Lachnospiraceae, Peptostreptococcaceae, Ruminococcaceae and Coriobacteriaceae were more prevalent. This study provides insight into the faecal bacterial community of wild young South American and Subantarctic fur seals. © FEMS 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Individual Foraging Strategies Reveal Niche Overlap between Endangered Galapagos Pinnipeds

PubMed Central

Villegas-Amtmann, Stella; Jeglinski, Jana W. E.; Costa, Daniel P.; Robinson, Patrick W.; Trillmich, Fritz

2013-01-01

Most competition studies between species are conducted from a population-level approach. Few studies have examined inter-specific competition in conjunction with intra-specific competition, with an individual-based approach. To our knowledge, none has been conducted on marine top predators. Sympatric Galapagos fur seals (Arctocephalus galapagoensis) and sea lions (Zalophus wollebaeki) share similar geographic habitats and potentially compete. We studied their foraging niche overlap at Cabo Douglas, Fernandina Island from simultaneously collected dive and movement data to examine spatial and temporal inter- and intra-specific competition. Sea lions exhibited 3 foraging strategies (shallow, intermediate and deep) indicating intra-specific competition. Fur seals exhibited one foraging strategy, diving predominantly at night, between 0–80 m depth and mostly at 19–22 h. Most sea lion dives also occurred at night (63%), between 0–40 m, within fur seals' diving depth range. 34% of sea lions night dives occurred at 19–22 h, when fur seals dived the most, but most of them occurred at dawn and dusk, when fur seals exhibited the least amount of dives. Fur seals and sea lions foraging behavior overlapped at 19 and 21 h between 0–30 m depths. Sea lions from the deep diving strategy exhibited the greatest foraging overlap with fur seals, in time (19 h), depth during overlapping time (21–24 m), and foraging range (37.7%). Fur seals foraging range was larger. Cabo Douglas northwest coastal area, region of highest diving density, is a foraging “hot spot” for both species. Fur seals and sea lions foraging niche overlap occurred, but segregation also occurred; fur seals primarily dived at night, while sea lions exhibited night and day diving. Both species exploited depths and areas exclusive to their species. Niche breadth generally increases with environmental uncertainty and decreased productivity. Potential competition between these species could be greater during warmer periods when prey availability is reduced. PMID:23967096

Urinary oxytocin in capuchin monkeys: Validation and the influence of social behavior.

PubMed

Benítez, Marcela E; Sosnowski, Meghan J; Tomeo, Olivia B; Brosnan, Sarah F

2018-05-24

In highly social species, like primates, oxytocin plays an important role in cooperation, and in the formation and maintenance of social relationships. Despite recent interest in the relationship between oxytocin and social behavior in nonhuman primates, relatively little is known about endogenous oxytocin in social New World Monkeys. In this paper, we investigate the relationship between oxytocin and affiliative behaviors in socially-housed captive capuchin monkeys (Sapajus [Cebus] apella) by first validating methods of analysis of urinary oxytocin in this species and, second, examining the effects of grooming and fur-rubbing behavior on oxytocin concentrations and further affiliative behavior. In the validation, we found that intranasal exogenous oxytocin significantly increased urinary oxytocin 15-60 min post-administration. Oxytocin was also implicated in both grooming and fur-rubbing behaviors. We found that oxytocin concentrations increased after subjects engaged in grooming or fur-rubbing. In addition, we found that fur-rubbing influenced affiliative behaviors, both during and after a social fur-rubbing bout. While individuals spent more time in contact and proximity while fur-rubbing, immediately following the fur-rubbing event (15-30 min afterwards) all affiliative behaviors decreased. This supports previous research that oxytocin may, in fact, initially be related to increased social distance in this species. Yet, an increase in all affiliative behaviors 30-45 min after the onset of fur-rubbing suggests that fur-rubbing, like grooming, may ultimately function to strengthen social relationships. Overall, these results support a critical role for oxytocin in affiliative behaviors that maintain and strengthen social relationships in capuchin monkeys, and highlight the complexity of the interactions among oxytocin, affiliative behaviors, and social bonding. © 2018 Wiley Periodicals, Inc.

77 FR 6682 - Marine Mammals; Subsistence Taking of Northern Fur Seals; Harvest Estimates

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-09

.... 110781394-2048-02] RIN 0648-BB09 Marine Mammals; Subsistence Taking of Northern Fur Seals; Harvest Estimates...), Commerce. ACTION: Final estimates of annual fur seal subsistence needs. SUMMARY: Pursuant to the regulations governing the subsistence taking of [[Page 6683

Using Fur to Estimate Mercury Concentrations in Mink

EPA Science Inventory

Total mercury (Hg) concentrations in fur and muscle tissue from mink (Mustela vison) were compared to determine the utility of fur analysis as a non-lethal and convenient method for predicting mercury concentrations in tissues. Sixty nine wild-trapped mink were collected in Rhode...

Development and Testing of Harpoon-Based Approaches for Collecting Comet Samples (Video Supplement)

NASA Technical Reports Server (NTRS)

Purves, Lloyd (Compiler); Nuth, Joseph (Compiler); Amatucci, Edward (Compiler); Wegel, Donald; Smith, Walter; Leary, James; Kee, Lake; Hill, Stuart; Grebenstein, Markus; Voelk, Stefan;

Deriving an atmospheric budget of total organic bromine using airborne in situ measurements from the western Pacific area during SHIVA

NASA Astrophysics Data System (ADS)

Sala, S.; Bönisch, H.; Keber, T.; Oram, D. E.; Mills, G.; Engel, A.

2014-07-01

During the recent SHIVA (Stratospheric Ozone: Halogen Impacts in a Varying Atmosphere) project an extensive data set of all halogen species relevant for the atmospheric budget of total organic bromine was collected in the western Pacific region using the Falcon aircraft operated by the German Aerospace agency DLR (Deutsches Zentrum für Luft- und Raumfahrt) covering a vertical range from the planetary boundary layer up to the ceiling altitude of the aircraft of 13 km. In total, more than 700 measurements were performed with the newly developed fully automated in situ instrument GHOST-MS (Gas chromatograph for the Observation of Tracers - coupled with a Mass Spectrometer) by the Goethe University of Frankfurt (GUF) and with the onboard whole-air sampler WASP with subsequent ground-based state-of-the-art GC / MS analysis by the University of East Anglia (UEA). Both instruments yield good agreement for all major (CHBr3 and CH2Br2) and minor (CH2BrCl, CHBrCl2 and CHBr2Cl) VSLS (very short-lived substances), at least at the level of their 2σ measurement uncertainties. In contrast to the suggestion that the western Pacific could be a region of strongly increased atmospheric VSLS abundance (Pyle et al., 2011), we found only in the upper troposphere a slightly enhanced amount of total organic bromine from VSLS relative to the levels reported in Montzka and Reimann et al. (2011) for other tropical regions. From the SHIVA observations in the upper troposphere, a budget for total organic bromine, including four halons (H-1301, H-1211, H-1202, H-2402), CH3Br and the VSLS, is derived for the level of zero radiative heating (LZRH), the input region for the tropical tropopause layer (TTL) and thus also for the stratosphere. With the exception of the two minor VSLS CHBrCl2 and CHBr2Cl, excellent agreement with the values reported in Montzka and Reimann et al. (2011) is found, while being slightly higher than previous studies from our group based on balloon-borne measurements.

Interplanetary CubeSats system for space weather evaluations and technology demonstration

NASA Astrophysics Data System (ADS)

Viscio, Maria Antonietta; Viola, Nicole; Corpino, Sabrina; Stesina, Fabrizio; Fineschi, Silvano; Fumenti, Federico; Circi, Christian

2014-11-01

The paper deals with the mission analysis and conceptual design of an interplanetary 6U CubeSats system to be implemented in the L1 Earth-Sun Lagrangian Point mission for solar observation and in-situ space weather measurements. Interplanetary CubeSats could be an interesting alternative to big missions, to fulfill both scientific and technological tasks in deep space, as proved by the growing interest in this kind of application in the scientific community and most of all at NASA. Such systems allow less costly missions, due to their reduced sizes and volumes, and consequently less demanding launches requirements. The CubeSats mission presented in this paper is aimed at supporting measurements of space weather. The mission envisages the deployment of a 6U CubeSats system in the L1 Earth-Sun Lagrangian Point, where solar observations for in situ measurements of space weather to provide additional warning time to Earth can be carried out. The proposed mission is also intended as a technology validation mission, giving the chance to test advanced technologies, such as telecommunications and solar sails, envisaged as propulsion system. Furthermore, traveling outside the Van Allen belts, the 6U CubeSats system gives the opportunity to further investigate the space radiation environment: radiation dosimeters and advanced materials are envisaged to be implemented, in order to test their response to the harsh space environment, even in view of future implementation on other spacecrafts (e.g. manned spacecrafts). The main issue related to CubeSats is how to fit big science within a small package - namely power, mass, volume, and data limitations. One of the objectives of the work is therefore to identify and size the required subsystems and equipment, needed to accomplish specific mission objectives, and to investigate the most suitable configuration, in order to be compatible with the typical CubeSats (multi units) standards. The work has been developed as collaboration between Politecnico di Torino, Sapienza University of Rome, "Osservatorio Astrofisico di Torino - INAF" (Astrophysical Observatory of Torino) and Deutsches Zentrum für Luft- und Raumfahrt (DLR) in Bremen.

Metagenomic Survey of Viral Diversity Obtained from Feces of Subantarctic and South American Fur Seals

PubMed Central

Kluge, Mariana; Campos, Fabrício Souza; Tavares, Maurício; de Amorim, Derek Blaese; Valdez, Fernanda Pedone; Giongo, Adriana; Roehe, Paulo Michel; Franco, Ana Claudia

2016-01-01

The Brazilian South coast seasonally hosts numerous marine species, observed particularly during winter months. Some animals, including fur seals, are found dead or debilitated along the shore and may harbor potential pathogens within their microbiota. In the present study, a metagenomic approach was performed to evaluate the viral diversity in feces of fur seals found deceased along the coast of the state of Rio Grande do Sul. The fecal virome of two fur seal species was characterized: the South American fur seal (Arctocephalus australis) and the Subantarctic fur seal (Arctocephalus tropicalis). Fecal samples from 10 specimens (A. australis, n = 5; A. tropicalis, n = 5) were collected and viral particles were purified, extracted and amplified with a random PCR. The products were sequenced through Ion Torrent and Illumina platforms and assembled reads were submitted to BLASTx searches. Both viromes were dominated by bacteriophages and included a number of potentially novel virus genomes. Sequences of picobirnaviruses, picornaviruses and a hepevirus-like were identified in A. australis. A rotavirus related to group C, a novel member of the Sakobuvirus and a sapovirus very similar to California sea lion sapovirus 1 were found in A. tropicalis. Additionally, sequences of members of the Anelloviridae and Parvoviridae families were detected in both fur seal species. This is the first metagenomic study to screen the fecal virome of fur seals, contributing to a better understanding of the complexity of the viral community present in the intestinal microbiota of these animals. PMID:26986573

75 FR 21243 - Marine Mammals; Subsistence Taking of Northern Fur Seals; St. George

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-23

... Mammals; Subsistence Taking of Northern Fur Seals; St. George AGENCY: National Marine Fisheries Service... (APA). The Pribilof Island Community of St. George Island, Traditional Council (Council) petitioned... St. George Island to take male fur seal young of the year during the fall. NMFS solicits public...

Evaluation of protective efficacy of live attenuated Salmonella enterica serovar Gallinarum vaccine strains against fowl typhoid in chickens.

PubMed

Laniewski, Paweł; Mitra, Arindam; Karaca, Kemal; Khan, Ayub; Prasad, Rajeev; Curtiss, Roy; Roland, Kenneth L

2014-09-01

Salmonella enterica serovar Gallinarum is the etiological agent of fowl typhoid, which constitutes a considerable economic problem for poultry growers in developing countries. The vaccination of chickens seems to be the most effective strategy to control the disease in those areas. We constructed S. Gallinarum strains with a deletion of the global regulatory gene fur and evaluated their virulence and protective efficacy in Rhode Island Red chicks and Brown Leghorn layers. The fur deletion mutant was avirulent and, when delivered orally to chicks, elicited excellent protection against lethal S. Gallinarum challenge. It was not as effective when given orally to older birds, although it was highly immunogenic when delivered by intramuscular injection. We also examined the effect of a pmi mutant and a combination of fur deletions with mutations in the pmi and rfaH genes, which affect O-antigen synthesis, and ansB, whose product inhibits host T-cell responses. The S. Gallinarum Δpmi mutant was only partially attenuated, and the ΔansB mutant was fully virulent. The Δfur Δpmi and Δfur ΔansB double mutants were attenuated but not protective when delivered orally to the chicks. However, a Δpmi Δfur strain was highly immunogenic when administered intramuscularly. All together, our results show that the fur gene is essential for the virulence of S. Gallinarum, and the fur mutant is effective as a live recombinant vaccine against fowl typhoid. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Entanglements of Consumption, Cruelty, Privacy, and Fashion: The Social Controversy over Fur.

ERIC Educational Resources Information Center

Olson, Kathryn M.; Goodnight, G. Thomas

1994-01-01

Posits a critical approach to the study of contemporary social controversy. Examines objectives to the use of fur as oppositional argument, rhetoric that veers from the goal of persuasion to block conventional associations and refashion communication norms. Shows how pro-fur responses illustrate strategies available to bolster, alter, or abandon…

Flexible ureterorenoscopy versus miniaturized PNL for solitary renal calculi of 10-30 mm size.

PubMed

Knoll, Thomas; Jessen, Jan Peter; Honeck, Patrick; Wendt-Nordahl, Gunnar

2011-12-01

The value of flexible ureterorenoscopy (fURS) and miniaturized PNL (mPNL) for larger renal calculi is under discussion. This non-randomized prospective study aimed to evaluate fURS and mPNL for solitary renal stones of 10-30 mm size. fURS was carried out in 21 patients with last generation 7.5F endoscopes. Ureteral access sheaths were used in 19 patients. For mPNL, an 18F modified Amplatz sheath with a 14F nephroscope were used (n = 25). The procedure was performed either tubeless with an antegrade stent or a nephrostomy. Outcome and complications of both procedures were assessed. Patients' demographics and stone sizes were comparable (18 ± 5 vs. 19 ± 4 mm, P = 0.08). Patients in the fURS group had a higher mean BMI (31 vs. 27, P < 0.05). Total OR time was significantly longer for fURS (106 ± 51 vs. 59 ± 19 min., P < 0.001). More patients were stone-free after one single percutaneous treatment, while 2nd-stage treatments with fURS were common (total procedures 1.04 vs. 1.52, P < 0.001; immediate stone-free rate (SFR) 96% vs. 71.5%, P < 0.001). SFR after 4 weeks was 100% (mPNL) and 85.8% (fURS) (P < 0.01). Minor complications as classified by Clavien I or II occurred in 16 and 23.8%, mPNL and fURS, respectively, P = 0.13). No major complications (Clavien III-V) occured in both groups. Our series supports both the concept of either percutaneous or retrograde endoscopic treatment for renal calculi with both modalities offering excellent safety. However, while for fURS, a significantly higher rate of 2nd-stage procedures was necessary, and mPNL led to faster and higher SFR without increasing complication rate.

Use of electrochemically activated aqueous solutions in the manufacture of fur materials.

PubMed

Danylkovych, Anatoliy G; Lishchuk, Viktor I; Romaniuk, Oksana O

2016-01-01

The influence of characteristics of electrochemically activated aqueous processing mediums in the treatment of fur skins with different contents of fatty substances was investigated. The use of electroactive water, namely anolytes and catholytes, forgoing antiseptics or surface-active materials, helped to restore the hydration of fur skins and to remove from them soluble proteins, carbohydrates and fatty substances. The activating effect of anolyte and catholyte in solutions of water on the processes of treating raw furs is explained by their special physical and chemical properties, namely the presence of free radicals, ions and molecules of water which easily penetrate cells' membranes and into the structure of non-collagen components and microfiber structure of dermic collagen. The stage of lengthy acid and salt treatment is excluded from the technical treatment as a result of using electroactivated water with high oxidizing power. A low-cost technology of processing different kinds of fur with the use of electroactivated water provides for substantial economy of water and chemical reagents, a two to threefold acceleration of the soaking and tanning processes and creation of highly elastic fur materials with a specified set of physical and chemical properties. At the same time the technology of preparatory processes of fur treatment excludes the use of such toxic antiseptics as formalin and sodium silicofluoride, which gives grounds to regard it as ecologically safe.

An investigation of the impact of Melbourne Zoo's "Seal-the-Loop" donate call-to-action on visitor satisfaction and behavior.

PubMed

Mellish, Sarah; Sanders, Ben; Litchfield, Carla A; Pearson, Elissa L

2017-05-01

Modern zoos are uniquely positioned to educate the public about environmental issues and promote conservation action. This report investigates the introduction of a donation request during an interactive fur seal presentation (as part of Melbourne Zoo's "Seal-the-Loop" initiative) on visitor satisfaction, perceptions of donation as a way to help wild fur seals, and donation behaviors. Comparisons are made between three groups surveyed upon exit: (1) viewed the interactive fur seal presentation prior to the donation request implementation (pledge-presentation: N = 86; see Mellish, Pearson, Sanders, and Litchfield []; International Zoo Yearbook 129:129-154); (2) viewed the interactive fur seal presentation including the donation request (donate-presentation: N = 82); and (3) viewed the fur seal exhibit and donation point but not the presentation and were not directly asked to make a donation (donate-exhibit: N = 82). Findings demonstrate visitor satisfaction with the interactive fur seal presentation was not negatively impacted following the implementation of the donate request (with >92% of pledge-presentation and donate-presentation visitors providing a "satisfied" or "very satisfied" rating). Only the donate-presentation visitors reported donation as a conservation action to help wild fur seals (19.18%; 0% for pledge-presentation visitors). While both donate-exhibit (39.51%) and donate-presentation visitors (60.75%) self-reported making donations or intending to do so, donation behavior was significantly increased for visitors who had viewed the fur seal presentation. Findings provide preliminary support that zoos may utilize interactive educational presentations to effectively ask visitors for donations to support specific conservation projects, without negatively impacting on satisfaction and with a relatively high level of visitor engagement. © 2017 Wiley Periodicals, Inc.

The Role of the Regulator Fur in Gene Regulation and Virulence of Riemerella anatipestifer Assessed Using an Unmarked Gene Deletion System

PubMed Central

Guo, Yunqing; Hu, Di; Guo, Jie; Li, Xiaowen; Guo, Jinyue; Wang, Xiliang; Xiao, Yuncai; Jin, Hui; Liu, Mei; Li, Zili; Bi, Dingren; Zhou, Zutao

2017-01-01

Riemerella anatipestifer, an avian pathogen, has resulted in enormous economic losses to the duck industry globally. Notwithstanding, little is known regarding the physiological, pathogenic and virulence mechanisms of Riemerella anatipestifer (RA) infection. However, the role of Ferric uptake regulator (Fur) in the virulence of R. anatipestifer has not, to date, been demonstrated. Using a genetic approach, unmarked gene deletion system, we evaluated the function of fur gene in the virulence of R. anatipestifer. For this purpose, we constructed a suicide vector containing pheS as a counter selectable marker for unmarked deletion of fur gene to investigate its role in the virulence. After successful transformation of the newly constructed vector, a mutant strain was characterized for genes regulated by iron and Fur using RNA-sequencing and a comparison was made between wild type and mutant strains in both iron restricted and enriched conditions. RNA-seq analysis of the mutant strain in a restricted iron environment showed the downregulation and upregulation of genes which were involved in either important metabolic pathways, transport processes, growth or cell membrane synthesis. Electrophoretic mobility shift assay was performed to identify the putative sequences recognized by Fur. The putative Fur-box sequence was 5′-GATAATGATAATCATTATC-3′. Lastly, the median lethal dose and histopathological investigations of animal tissues also illustrated mild pathological lesions produced by the mutant strain as compared to the wild type RA strain, hence showing declined virulence. Conclusively, an unmarked gene deletion system was successfully developed for RA and the role of the fur gene in virulence was explored comprehensively. PMID:28971067

Comparison of the effects of tolvaptan and furosemide on renal water and sodium excretion in patients with heart failure and advanced chronic kidney disease: a subanalysis of the K-STAR study.

PubMed

Tominaga, Naoto; Kida, Keisuke; Inomata, Takayuki; Sato, Naoki; Izumi, Tohru; Akashi, Yoshihiro J; Shibagaki, Yugo

2018-06-22

Tolvaptan (TLV) is known to increase electrolyte-free water clearance. However, TLV actions on renal electrolytes including urine sodium (uNa) excretion and its consequences are less well understood. This subanalysis investigated the effect of add-on TLV compared to increased furosemide (FUR) on both electrolyte-free water and electrolyte clearance in patients with congestive heart failure (CHF) complicated by advanced chronic kidney disease (CKD). The Kanagawa Aquaresis Investigators Trial of TLV on HF Patients with Renal Impairment (K-STAR) was a multicenter, open-labeled, randomized, and controlled prospective clinical study. Eighty-one Japanese patients with CHF and residual signs of congestion despite oral FUR treatment (≥ 40 mg/day) were recruited and randomly assigned to a 7-day add-on treatment with either ≤ 40 mg/day FUR or ≤ 15 mg/day TLV. Electrolyte-free water clearance, electrolyte osmolar clearance and electrolyte excretion were compared between the two groups before and after therapy. The change (Δ) in electrolyte-free water clearance was significantly higher in the add-on TLV group than in the add-on FUR group. However, Δelectrolyte osmolar clearance was also higher in the add-on TLV group than in the increased FUR group. This was primarily because ΔuNa excretion was significantly higher in the add-on TLV group than in the increased FUR group, since Δurine potassium excretion was significantly lower in the add-on TLV group than in the increased FUR group. Add-on TLV may increase both renal water and Na excretion in CHF patients with advanced CKD to a greater degree than increased FUR.

Correlations between elements in the fur of wild animals.

PubMed

Długaszek, Maria; Kopczyński, Krzysztof

2014-07-01

There is little data on the elemental composition of wild animals fur. In the paper, an attempt has been made to evaluate the concentration of elements in the fur of roe deer, wild boar and hare. The contents of following elements: calcium (Ca), magnesium (Mg), zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), lead (Pb), cadmium (Cd), aluminium (Al), chromium (Cr), nickel (Ni) were determined by atomic absorption spectrometry method. Their content was in the range 0.01 (Cd) to 1,519 (Ca) μg/g. Correlations between the content of Mn, Al, Ca, Pb, Cr, Ni in the fur of animals, liver and muscle tissues were found. Thus it can be assumed that the fur of wild animals can provide an information on the bioavailability of elements and environmental exposure and can be considered as an useful biomarker in animals and environmental studies, although research on this subject should be continued.

A memory like a female Fur Seal: long-lasting recognition of pup's voice by mothers.

PubMed

Mathevon, Nicolas; Charrier, Isabelle; Aubin, Thierry

2004-06-01

In colonial mammals like fur seals, mutual vocal recognition between mothers and their pup is of primary importance for breeding success. Females alternate feeding sea-trips with suckling periods on land, and when coming back from the ocean, they have to vocally find their offspring among numerous similar-looking pups. Young fur seals emit a 'mother-attraction call' that presents individual characteristics. In this paper, we review the perceptual process of pup's call recognition by Subantarctic Fur Seal Arctocephalus tropicalis mothers. To identify their progeny, females rely on the frequency modulation pattern and spectral features of this call. As the acoustic characteristics of a pup's call change throughout the lactation period due to the growing process, mothers have thus to refine their memorization of their pup's voice. Field experiments show that female Fur Seals are able to retain all the successive versions of their pup's call.

16 CFR 303.9 - Use of fur-bearing animal names and symbols prohibited.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Use of fur-bearing animal names and symbols prohibited. 303.9 Section 303.9 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE TEXTILE FIBER PRODUCTS IDENTIFICATION ACT § 303.9 Use of fur-bearing animal names and symbols...

Isolation and characterization of Campylobacter spp. from Antarctic fur seals (Arctocephalus gazella) at Deception Island, Antarctica.

PubMed

García-Peña, F J; Pérez-Boto, D; Jiménez, C; San Miguel, E; Echeita, A; Rengifo-Herrera, C; García-Párraga, D; Ortega-Mora, L M; Pedraza-Díaz, S

2010-09-01

The presence of Campylobacter spp. was investigated in 41 Antarctic fur seals (Arctocephalus gazella) and 9 Weddell seals (Leptonychotes weddellii) at Deception Island, Antarctica. Infections were encountered in six Antarctic fur seals. The isolates, the first reported from marine mammals in the Antarctic region, were identified as Campylobacter insulaenigrae and Campylobacter lari.

Iron regulates expression of Bacillus cereus hemolysin II via global regulator Fur.

PubMed

Sineva, Elena; Shadrin, Andrey; Rodikova, Ekaterina A; Andreeva-Kovalevskaya, Zhanna I; Protsenko, Alexey S; Mayorov, Sergey G; Galaktionova, Darya Yu; Magelky, Erica; Solonin, Alexander S

2012-07-01

The capacity of pathogens to respond to environmental signals, such as iron concentration, is key to bacterial survival and establishment of a successful infection. Bacillus cereus is a widely distributed bacterium with distinct pathogenic properties. Hemolysin II (HlyII) is one of its pore-forming cytotoxins and has been shown to be involved in bacterial pathogenicity in a number of cell and animal models. Unlike many other B. cereus pathogenicity factors, HlyII is not regulated by pleiotropic transcriptional regulator PlcR but is controlled by its own regulator, HlyIIR. Using a combination of in vivo and in vitro techniques, we show that hlyII expression is also negatively regulated by iron by the global regulator Fur via direct interaction with the hlyII promoter. DNase I footprinting and in vitro transcription experiments indicate that Fur prevents RNA polymerase binding to the hlyII promoter. HlyII expression profiles demonstrate that both HlyIIR and Fur regulate HlyII expression in a concerted fashion, with the effect of Fur being maximal in the early stages of bacterial growth. In sum, these results show that Fur serves as a transcriptional repressor for hlyII expression.

Flexible ureterorenoscopy and laser lithotripsy in children

PubMed Central

Yeow, When-Chan; Pemberton, Richard; Barker, Andrew

2009-01-01

Background: Flexible ureterorenoscopy (FUR) and laser lithotripsy (LL) are techniques used in the management of upper urinary tract disorders. These techniques, so far established in adults, are now being used in children as well. We report our experience with 26 cases of pediatric upper urinary tract disorders treated using these techniques. Methods: In the period from 1997 to 2006, FUR was performed in 26 children (14 males and 12 females) in the age group of three months to 15 years with a mean age of 8.2 years. Twenty five were stented prior to undergoing FUR and 24 presented with suspected upper tract stones (17 pelvicalyceal and seven midureteric). Two cases showed JJ stent migration post-pyeloplasty. Results: Eight cases involved diagnostic procedures. Six excluded the presence of renal calculi, one had focal medullary sponge kidney, and one had calcified papillae. There were 15 cases of therapeutic FUR. Of these, 12 had LL with only one had incomplete stone fragmentation which subsequently passed spontaneously. Other therapeutic procedures included removal of migrated JJ stents and FUR with the basket removal of a midureteric calculus. Three cases failed ureterorenoscopy due to technical difficulties. The overall success rate was 88.5% for FUR. Conclusion: FUR and LL are valuable minimally invasive techniques for the examination and treatment of pediatric upper urinary tract conditions. Preoperative stenting improves passage of the ureteroscope and with progressive miniaturization of instruments, the lower weight limit will decrease. PMID:20671848

25 CFR 309.18 - What are examples of hide, leatherwork, and fur that are Indian products?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 25 Indians 2 2011-04-01 2011-04-01 false What are examples of hide, leatherwork, and fur that are Indian products? 309.18 Section 309.18 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.18 What are examples of hide, leatherwork, and fur that...

25 CFR 309.18 - What are examples of hide, leatherwork, and fur that are Indian products?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false What are examples of hide, leatherwork, and fur that are Indian products? 309.18 Section 309.18 Indians INDIAN ARTS AND CRAFTS BOARD, DEPARTMENT OF THE INTERIOR PROTECTION OF INDIAN ARTS AND CRAFTS PRODUCTS § 309.18 What are examples of hide, leatherwork, and fur that...

Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery

DOE PAGES

Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; ...

2015-11-28

In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimummore » detectable activity concentrations of 137Cs and 134Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq 134Cs kg -1 f.w. (95% CI: 35.9–38.5) and 141.2 mBq 137Cs kg -1f.w. (95% CI: 135.5–146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25–0.28). The Fukushima nuclear accident released 134Cs and 137Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both 134Cs and 137Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace 134Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation exposure, either in northern fur seal or human populations consuming this species.« less

Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette

In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimummore » detectable activity concentrations of 137Cs and 134Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq 134Cs kg -1 f.w. (95% CI: 35.9–38.5) and 141.2 mBq 137Cs kg -1f.w. (95% CI: 135.5–146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25–0.28). The Fukushima nuclear accident released 134Cs and 137Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both 134Cs and 137Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace 134Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation exposure, either in northern fur seal or human populations consuming this species.« less

An Upstream Truncation of the furA-katG Operon Confers High-Level Isoniazid Resistance in a Mycobacterium tuberculosis Clinical Isolate with No Known Resistance-Associated Mutations

PubMed Central

Yam, Wing Cheong; Zhang, Ying; Kao, Richard Y. T.

2014-01-01

Although the major causes of isoniazid (INH) resistance in Mycobacterium tuberculosis are confined to structural mutations in katG and promoter mutations in the mabA-inhA operon, a significant proportion of INH-resistant strains have unknown resistance mechanisms. Recently, we identified a high-level INH-resistant M. tuberculosis clinical isolate, GB005, with no known resistance-associated mutations. A comprehensive study was performed to investigate the molecular basis of drug resistance in this strain. Although no mutations were found throughout the katG and furA-katG intergenic region, the katG expression and the catalase activity were greatly diminished compared to those in H37Rv (P < 0.01). Northern blotting revealed that the katG transcript from the isolate was smaller than that of H37Rv. Sequencing analysis of furA and upstream genes discovered a 7.2-kb truncation extended from the 96th base preceding the initiation codon of katG. Complementation of the M. tuberculosis Δ(furA-katG) strain with katG and different portions of the truncated region identified a 134-bp upstream fragment of furA that was essential for full catalase activity and INH susceptibility in M. tuberculosis. The promoter activity of this fragment was also shown to be stronger than that of the furA-katG intergenic region (P < 0.01). Collectively, these findings demonstrate that deletion of the 134-bp furA upstream fragment is responsible for the reduction in katG expression, resulting in INH resistance in GB005. To our knowledge, this is the first report showing that deletion of the upstream region preceding the furA-katG operon causes high-level INH resistance in a clinical isolate of M. tuberculosis. PMID:25092698

Fukushima derived radiocesium in subsistence-consumed northern fur seal and wild celery.

PubMed

Ruedig, Elizabeth; Duncan, Colleen; Dickerson, Bobette; Williams, Michael; Gelatt, Thomas; Bell, Justin; Johnson, Thomas E

2016-02-01

In July 2014, our investigative team traveled to St. Paul Island, Alaska to measure concentrations of radiocesium in wild-caught food products, primarily northern fur seal (Callorhinus ursinus). The 2011 Fukushima Daiichi Nuclear Power Plant accident released radiocesium into the atmosphere and into the western Pacific Ocean; other investigators have detected Fukushima-derived radionuclides in a variety of marine products harvested off the western coast of North America. We tested two subsistence-consumed food products from St. Paul Island, Alaska for Fukushima-derived radionuclides: 54 northern fur seal, and nine putchki (wild celery, Angelica lucida) plants. Individual northern fur seal samples were below minimum detectable activity concentrations of (137)Cs and (134)Cs, but when composited, northern fur seal tissues tested positive for trace quantities of both isotopes. Radiocesium was detected at an activity concentration of 37.2 mBq (134)Cs kg(-1) f.w. (95% CI: 35.9-38.5) and 141.2 mBq (137)Cs kg(-1) f.w. (95% CI: 135.5-146.8). The measured isotopic ratio, decay-corrected to the date of harvest, was 0.26 (95% CI: 0.25-0.28). The Fukushima nuclear accident released (134)Cs and (137)Cs in roughly equal quantities, but by the date of harvest in July 2014, this ratio was 0.2774, indicating that this population of seals has been exposed to small quantities of Fukushima-derived radiocesium. Activity concentrations of both (134)Cs and (137)Cs in putchki were below detection limits, even for composited samples. Northern fur seal is known to migrate between coastal Alaska and Japan and the trace (134)Cs in northern fur seal tissue suggests that the population under study had been minimally exposed Fukushima-derived radionuclides. Despite this inference, the radionuclide quantities detected are small and no impact is expected as a result of the measured radiation exposure, either in northern fur seal or human populations consuming this species. Published by Elsevier Ltd.

Drafting Instructors Look at Metrics, Computers, and Change

ERIC Educational Resources Information Center

Smith, Howard

1974-01-01

Four drafting teachers give their opinions on how to teach metric measurement and conversion and other matters. They are Jeffry T. Lufting, James E. Kelso, L. Dayle Yeager, and Michael B. Atkins. Computer graphics, modular structures, communicative drafting, and microfilm reproduction are also discussed. (MS)

Hindering the illegal trade in dog and cat furs through a DNA-based protocol for species identification.

PubMed

Garofalo, Luisa; Mariacher, Alessia; Fanelli, Rita; Fico, Rosario; Lorenzini, Rita

2018-01-01

In Western countries dogs and cats are the most popular pets, and people are increasingly opposed to their rearing for the fur industry. In 2007, a Regulation of the European Union (EU) banned the use and trade of dog and cat furs, but an official analytical protocol to identify them as source species was not provided, and violations of law are still frequent in all Member States. In this paper we report on the development and validation of a simple and affordable DNA method for species detection in furs to use as an effective tool to combat illegal trade in fur products. A set of mitochondrial primers was designed for amplification of partial cytochrome b, control region and ND1 gene in highly degraded samples, like furs and pelts. Our amplification workflow involved the use of a non-specific primer pair to perform a first test to identify the species through sequencing, then the application of species-specific primer pairs to use in singleplex end-point PCRs as confirmation tests. The advantage of this two-step procedure is twofold: on the one hand it minimises the possibility of negative test results from degraded samples, since failure of amplification with a first set of primers can be offset by successful amplification of the second, and on the other it adds confidence and reliability to final authentication of species. All designed primers were validated on a reference collection of tissue samples, obtaining solid results in terms of specificity, sensitivity, repeatability and reproducibility. Application of the protocol on real caseworks from seized furs yielded successful results also from old and dyed furs, suggesting that age and chemical staining do not necessarily affect positive amplifications. Major pros of this approach are: (1) sensitive and informative primer sets for detection of species; (2) short PCR amplicons for the analysis of poor quality DNA; (3) binding primers that avoid contamination from human DNA; (4) user-friendly protocol for any laboratory equipped for analysis of low-copy-number DNA. Our molecular procedure proved to be a good starting point for enforcing the EU Regulation against dog and cat fur trade in forensic contexts where source attribution is essential to the assignment of responsibilities.

Intrinsic and extrinsic influences on standard metabolic rates of three species of Australian otariid

PubMed Central

Slip, David J.; Harcourt, Robert G.

2017-01-01

Abstract The study of marine mammal energetics can shed light on how these animals might adapt to changing environments. Their physiological potential to adapt will be influenced by extrinsic factors, such as temperature, and by intrinsic factors, such as sex and reproduction. We measured the standard metabolic rate (SMR) of males and females of three Australian otariid species (two Australian fur seals, three New Zealand fur seals and seven Australian sea lions). Mean SMR ranged from 0.47 to 1.05 l O2 min−1, which when adjusted for mass was from 5.33 to 7.44 ml O2 min−1 kg−1. We found that Australian sea lion mass-specific SMR (sSMR; in millilitres of oxygen per minute per kilogram) varied little in response to time of year or moult, but was significantly influenced by sex and water temperature. Likewise, sSMR of Australian and New Zealand fur seals was also influenced by sex and water temperature, but also by time of year (pre-moult, moult or post-moult). During the moult, fur seals had significantly higher sSMR than at other times of the year, whereas there was no discernible effect of moult for sea lions. For both groups, females had higher sSMR than males, but sea lions and fur seals showed different responses to changes in water temperature. The sSMR of fur seals increased with increasing water temperature, whereas sSMR of sea lions decreased with increasing water temperature. There were no species differences when comparing animals of the same sex. Our study suggests that fur seals have more flexibility in their physiology than sea lions, perhaps implying that they will be more resilient in a changing environment. PMID:28852504

A Bayesian hierarchical model of Antarctic fur seal foraging and pup growth related to sea ice and prey abundance.

PubMed

Hiruki-Raring, Lisa M; Ver Hoef, Jay M; Boveng, Peter L; Bengtson, John L

2012-03-01

We created a Bayesian hierarchical model (BHM) to investigate ecosystem relationships between the physical ecosystem (sea ice extent), a prey measure (krill density), predator behaviors (diving and foraging effort of female Antarctic fur seals, Arctocephalus gazella, with pups) and predator characteristics (mass of maternal fur seals and pups). We collected data on Antarctic fur seals from 1987/1988 to 1994/1995 at Seal Island, Antarctica. The BHM allowed us to link together predators and prey into a model that uses all the data efficiently and accounts for major sources of uncertainty. Based on the literature, we made hypotheses about the relationships in the model, which we compared with the model outcome after fitting the BHM. For each BHM parameter, we calculated the mean of the posterior density and the 95% credible interval. Our model confirmed others' findings that increased sea ice was related to increased krill density. Higher krill density led to reduced dive intensity of maternal fur seals, as measured by dive depth and duration, and to less time spent foraging by maternal fur seals. Heavier maternal fur seals and lower maternal foraging effort resulted in heavier pups at 22 d. No relationship was found between krill density and maternal mass, or between maternal mass and foraging effort on pup growth rates between 22 and 85 days of age. Maternal mass may have reflected environmental conditions prior to the pup provisioning season, rather than summer prey densities. Maternal mass and foraging effort were not related to pup growth rates between 22 and 85 d, possibly indicating that food was not limiting, food sources other than krill were being used, or differences occurred before pups reached age 22 d.

Discovery of fur seal feces-associated circular DNA virus in swine feces in Japan.

PubMed

Oba, Mami; Katayama, Yukie; Naoi, Yuki; Tsuchiaka, Shinobu; Omatsu, Tsutomu; Okumura, Atsushi; Nagai, Makoto; Mizutani, Tetsuya

2017-10-07

Fur seal feces-associated circular ssDNA virus (FSfaCV) was discovered in a pig for the first time in Japan using a next-generation sequencer with duplex-specific nuclease. Full genome of the virus showed approximately 92% similarity to FSfaCVs from New Zealand fur seals. Furthermore, we investigated the prevalence of the ssDNA virus in 85 piglets in Japan, and 65 piglets were positive (76%) for the virus.

Sequential induction of Fur-regulated genes in response to iron limitation in Bacillus subtilis.

PubMed

Pi, Hualiang; Helmann, John D

2017-11-28

Bacterial cells modulate transcription in response to changes in iron availability. The ferric uptake regulator (Fur) senses intracellular iron availability and plays a central role in maintaining iron homeostasis in Bacillus subtilis Here we utilized FrvA, a high-affinity Fe 2+ efflux transporter from Listeria monocytogenes , as an inducible genetic tool to deplete intracellular iron. We then characterized the responses of the Fur, FsrA, and PerR regulons as cells transition from iron sufficiency to deficiency. Our results indicate that the Fur regulon is derepressed in three distinct waves. First, uptake systems for elemental iron ( efeUOB ), ferric citrate ( fecCDEF ), and petrobactin ( fpbNOPQ ) are induced to prevent iron deficiency. Second, B. subtilis synthesizes its own siderophore bacillibactin ( dhbACEBF ) and turns on bacillibactin ( feuABC ) and hydroxamate siderophore ( fhuBCGD ) uptake systems to scavenge iron from the environment and flavodoxins ( ykuNOP ) to replace ferredoxins. Third, as iron levels decline further, an "iron-sparing" response ( fsrA , fbpAB , and fbpC ) is induced to block the translation of abundant iron-utilizing proteins and thereby permit the most essential iron-dependent enzymes access to the limited iron pools. ChIP experiments demonstrate that in vivo occupancy of Fur correlates with derepression of each operon, and the graded response observed here results, at least in part, from higher-affinity binding of Fur to the "late"-induced genes.

Influence of myristic acid on furosemide binding to bovine serum albumin. Comparison with furosemide-human serum albumin complex

NASA Astrophysics Data System (ADS)

Bojko, B.; Sułkowska, A.; Maciążek-Jurczyk, M.; Równicka, J.; Sułkowski, W. W.

2010-06-01

Fluorescence studies on furosemide (FUR) binding to bovine serum albumin (BSA) showed the existence of three or four binding sites in the tertiary structure of the protein. Two of them are located in subdomain IIA, while the others in subdomains IB and/or IIIA. Furosemide binding in subdomain IB is postulated on the basis of run of Stern-Volmer plot indicating the existence of two populations of tryptophans involved in the interaction with FUR. In turn, the significant participation of tyrosil residues in complex formation leads to the consideration of the subdomain IIIA as furosemide low-affinity binding site. The effect of increasing concentration of fatty acid on FUR binding in all studied binding sites was also investigated and compared with the previous results obtained for human serum albumin (HSA). For BSA the lesser impact of fatty acid on affinity between drug and albumin was observed. This is probably a result of more significant role of tyrosines in the complex formation and different polarity of microenvironment of the fluorophores when compared HSA and BSA. The most distinct differences between FUR-BSA and FUR-HSA binding parameters are observed when third fatty acid molecule is bound with the protein and rotation of domains I and II occurs. However these structural changes mostly affect FUR low affinity binding sites.

The behavioural response of Australian fur seals to motor boat noise.

PubMed

Tripovich, Joy S; Hall-Aspland, Sophie; Charrier, Isabelle; Arnould, John P Y

2012-01-01

Australian fur seals breed on thirteen islands located in the Bass Strait, Australia. Land access to these islands is restricted, minimising human presence but boat access is still permissible with limitations on approach distances. Thirty-two controlled noise exposure experiments were conducted on breeding Australian fur seals to determine their behavioural response to controlled in-air motor boat noise on Kanowna Island (39°10'S, 146°18'E). Our results show there were significant differences in the seals' behaviour at low (64-70 dB) versus high (75-85 dB) sound levels, with seals orientating themselves towards or physically moving away from the louder boat noise at three different sound levels. Furthermore, seals responded more aggressively with one another and were more alert when they heard louder boat noise. Australian fur seals demonstrated plasticity in their vocal responses to boat noise with calls being significantly different between the various sound intensities and barks tending to get faster as the boat noise got louder. These results suggest that Australian fur seals on Kanowna Island show behavioural disturbance to high level boat noise. Consequently, it is recommended that an appropriate level of received boat sound emissions at breeding fur seal colonies be below 74 dB and that these findings be taken into account when evaluating appropriate approach distances and speed limits for boats.

The Behavioural Response of Australian Fur Seals to Motor Boat Noise

PubMed Central

Tripovich, Joy S.; Hall-Aspland, Sophie; Charrier, Isabelle; Arnould, John P. Y.

2012-01-01

Australian fur seals breed on thirteen islands located in the Bass Strait, Australia. Land access to these islands is restricted, minimising human presence but boat access is still permissible with limitations on approach distances. Thirty-two controlled noise exposure experiments were conducted on breeding Australian fur seals to determine their behavioural response to controlled in-air motor boat noise on Kanowna Island (39°10′S, 146°18′E). Our results show there were significant differences in the seals' behaviour at low (64–70 dB) versus high (75–85 dB) sound levels, with seals orientating themselves towards or physically moving away from the louder boat noise at three different sound levels. Furthermore, seals responded more aggressively with one another and were more alert when they heard louder boat noise. Australian fur seals demonstrated plasticity in their vocal responses to boat noise with calls being significantly different between the various sound intensities and barks tending to get faster as the boat noise got louder. These results suggest that Australian fur seals on Kanowna Island show behavioural disturbance to high level boat noise. Consequently, it is recommended that an appropriate level of received boat sound emissions at breeding fur seal colonies be below 74 dB and that these findings be taken into account when evaluating appropriate approach distances and speed limits for boats. PMID:22623998

Hematology, Serum Chemistry, and Early Hematologic Changes in Free-Ranging South American Fur Seals ( Arctocephalus australis ) at Guafo Island, Chilean Patagonia.

PubMed

Seguel, Mauricio; Muñoz, Francisco; Keenan, Alessandra; Perez-Venegas, Diego J; DeRango, Eugene; Paves, Hector; Gottdenker, Nicole; Müller, Ananda

2016-07-01

The establishment of clinical pathology baseline data is critical to evaluate temporal and spatial changes in marine mammal groups. Despite increased availability of studies on hematology and biochemistry of marine mammals, reference ranges are lacking for many populations, especially among fur seal species. During the austral summers of 2014 and 2015, we evaluated basic hematologic and biochemical parameters in clinically healthy, physically restrained South American fur seal ( Arctocephalus australis ) lactating females and 2-mo-old pups. We also assessed the temporal variation of hematology parameters on the pups during their first 2 mo of life. Reference ranges of lactating females were similar to those previously reported in other fur seal species. In the case of pups, reference ranges are similar to values previously reported in sea lion species. As expected, most biochemical and hematologic values differ significantly between adult females and pups. As in other otariids, South American fur seals pups are born with higher values of total red blood cells, hemoglobin, and packed cell volume, and lower numbers of total leukocytes, neutrophils, lymphocytes, and eosinophils. To the best of our knowledge, data on hematology reference values for South American fur seals has not been previously reported and is useful for continued health monitoring of this species, as well as for comparisons with other otariid groups.

First report of Echinococcus granulosus (genotype G6) in a dog in Bamako, Mali.

PubMed

Mauti, S; Traoré, A; Crump, L; Zinsstag, J; Grimm, F

2016-02-15

Cystic echinococcosis is one of the most widespread and important helminthic zoonoses, caused by the larval stage of Echinococcus granulosus sensu lato. However, to date there is little information about the disease in West Africa. Faecal and fur samples from 193 dogs, the main final hosts, were collected in 2010 and 2011 in Bamako, Mali. Taeniid eggs were found microscopically in 28/118 (24%) and 80/223 (36%) faecal and fur samples, respectively. One faecal and one fur sample from the same dog were positive for E. granulosus s. l. DNA. In the remaining 27 faecal (96%) and 77 fur samples (96%) only Taenia DNA was detected. Three microscopically positive fur samples were negative by PCR. Sequence analysis of part of the NADH dehydrogenase subunit 1 gene identified the parasite as E. granulosus (genotype G6; Echinococcus canadensis). This is the first study to focus on the final host of E. granulosus s. l. in Mali and the first report of E. canadensis in Mali. Copyright © 2016 Elsevier B.V. All rights reserved.

Historical analysis of Newfoundland dog fur colour genetics

PubMed Central

Bondeson, J.

2015-01-01

This article makes use of digitized historic newspapers to analyze Newfoundland dog fur colour genetics, and fur colour variations over time. The results indicate that contrary to the accepted view, the ‘Solid’ gene was introduced into the British population of Newfoundland dogs in the 1840s. Prior to that time, the dogs were white and black (Landseer) or white and brown, and thus spotted/spotted homozygotes. Due to ‘Solid’ being dominant over ‘spotted’, and selective breeding, today the majority of Newfoundland dogs are solid black. Whereas small white marks on the chest and/or paw appears to be a random event, the historical data supports the existence of an ‘Irish spotted’ fur colour pattern, with white head blaze, breast, paws and tail tip, in spotted/spotted homozygotes. PMID:26623371

Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells

PubMed Central

Yao, Cheng Wen; Kang, Kyoung Ah; Piao, Mei Jing; Ryu, Yea Seong; Fernando, Pattage Madushan Dilhara Jayatissa; Oh, Min Chang; Park, Jeong Eon; Shilnikova, Kristina; Na, Soo-Young; Jeong, Seung Uk; Boo, Sun-Jin; Hyun, Jin Won

2017-01-01

We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells. PMID:27737524

77 FR 16968 - Airworthiness Directives; Burkhart GROB Luft- und Raumfahrt GmbH Powered Sailplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-23

... verbal contact we receive about this proposed AD. Discussion The European Aviation Safety Agency (EASA...-200. (h) Related Information Refer to MCAI European Aviation Safety Agency (EAS) AD No. 2012- 0027...- und Raumfahrt GmbH Powered Sailplanes AGENCY: Federal Aviation Administration (FAA), Department of...

Physik gestern und heute: Visualisierung mit der Schlierenmethode

NASA Astrophysics Data System (ADS)

Heering, Peter

2006-07-01

Der Name des österreichischen Forschers Ernst Mach ist heute noch mit der Schallgeschwindigkeit verbunden. Diese Auszeichnung resultiert aus Machs Untersuchungen, wie sich Projektile mit Überschallgeschwindigkeit durch die Luft bewegen. Gerade in jüngster Zeit hat die Anwendung derartiger Methoden durch technische Modifikationen wieder einen Aufschwung erfahren.

76 FR 48814 - Applications for New Awards; Postsecondary Education Center for Individuals Who Are Deaf

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-09

... Institute on Disability and Rehabilitation Research, the Institute of Education Sciences, and the Office of...: Research priorities in the new millennium. Journal of Deaf Studies and Deaf Education, 7 (4), 267-280. Luft... DEPARTMENT OF EDUCATION Applications for New Awards; Postsecondary Education Center for...

DISCHARGE DEVICE FOR RADIOACTIVE MATERIAL

DOEpatents

Ohlinger, L.A.

1958-09-23

A device is described fur unloading bodies of fissionable material from a neutronic reactor. It is comprised essentially of a wheeled flat car having a receptacle therein containing a liquid coolant fur receiving and cooling the fuel elements as they are discharged from the reactor, and a reciprocating plunger fur supporting the fuel element during discharge thereof prior to its being dropped into the coolant. The flat car is adapted to travel along the face of the reactor adjacent the discharge ends of the coolant tubes.

Effect of 60Co-gamma radiation on the properties of furs

NASA Astrophysics Data System (ADS)

Raina, R. K.; Wali, B. K.; Wani, A. M.

Furs pretanned with various combinations of vegetable tanning agents and retanned with alum have been irradiated with 60Co γ-radiation in the dose range 5.0-114.0 kGy. The physico-chemical modifications induced by the radiation have been assessed by measuring changes in tensile strength, absorption of water, elongation and shrinkage temperature. For investigations, samples have been taken from the same topographic region of the rabbit furs, belonging to the same age and sex. The results are discussed hereunder.

Flood Control, Mississippi River at Prairie du Chien, Wisconsin.

DTIC Science & Technology

1977-02-01

The health and saftey of residents in the project area are potentially affected during major floods. A serious threat to life is always present...Warehouse (c. 1835) on St. Friol Island was one of the foremost establishments of John Jacob Astor’s American Fur Company . This old rock warehouse...prominant fur trader in the late 1700’s and early 1800’s. He served as the principal agent for the American Fur Company from 1820 to 182. °fhe house is a

Questionnaire survey of detrimental fur animal epidemic necrotic pyoderma in Finland.

PubMed

Nordgren, Heli; Vapalahti, Katariina; Vapalahti, Olli; Sukura, Antti; Virtala, Anna-Maija

2017-08-03

In 2007, a previously unrecorded disease, fur animal epidemic necrotic pyoderma (FENP), was detected in farmed mink (Neovision vision), foxes (Vulpes lagopus) and Finnraccoons (Nyctereutes procyonoides) in Finland. Symptoms included severe pyoderma with increased mortality, causing both animal welfare problems and economic losses. In 2011, an epidemiologic questionnaire was mailed to all members of the Finnish Fur Breeders' Association to assess the occurrence of FENP from 2009 through the first 6 months of 2011. The aim was to describe the geographical distribution and detailed clinical signs of FENP, as well as sources of infection and potential risk factors for the disease. A total of 239 farmers (25%) returned the questionnaire. Clinical signs of FENP were observed in 40% (95% CI 34-46%) of the study farms. In addition, the survey clarified the specific clinical signs for different animal species. The presence of disease was associated with the importation of mink, especially from Denmark (OR 9.3, 95% CI 2.6-33.0). The transmission route between Finnish farms was associated with fur animal purchases. Some risk factors such as the farm type were also indicated. As such, FENP was detected more commonly on farms with more than one species of fur animal in comparison to farms with, for example, only foxes (OR 4.6, 95% CI 2.4-8.6), and the incidence was higher on farms with over 750 breeder mink compared to smaller farms (OR 3.8, 95% CI 1.6-9.0). Contact between fur animals and birds and other wildlife increased the risk of FENP on farms. Responses also indicated that blocking the entry of wildlife to the animal premises protected against FENP. FENP was most likely introduced to Finland by imported mink and spread further within the country via domestically purchased fur animals. Some potential risk factors, such as the type and size of the farm and contact with wildlife, contributed to the spread of FENP. Escape-proof shelter buildings block the entry of wildlife, thus protecting fur animals against FENP.

26 CFR 1.1402(a)-13 - Income from agricultural activity.

Code of Federal Regulations, 2011 CFR

2011-04-01

... produce agricultural or horticultural commodities (including livestock, bees, poultry, and fur-bearing... livestock, bees, poultry, and fur-bearing animals and wildlife) on such land, and that there shall be...

26 CFR 1.1402(a)-13 - Income from agricultural activity.

Code of Federal Regulations, 2014 CFR

2014-04-01

... produce agricultural or horticultural commodities (including livestock, bees, poultry, and fur-bearing... livestock, bees, poultry, and fur-bearing animals and wildlife) on such land, and that there shall be...

26 CFR 1.1402(a)-13 - Income from agricultural activity.

Code of Federal Regulations, 2012 CFR

2012-04-01

... produce agricultural or horticultural commodities (including livestock, bees, poultry, and fur-bearing... livestock, bees, poultry, and fur-bearing animals and wildlife) on such land, and that there shall be...

26 CFR 1.1402(a)-13 - Income from agricultural activity.

Code of Federal Regulations, 2013 CFR

2013-04-01

... produce agricultural or horticultural commodities (including livestock, bees, poultry, and fur-bearing... livestock, bees, poultry, and fur-bearing animals and wildlife) on such land, and that there shall be...

Identifying Surface Changes on HRSC Images of the Mars South Polar Residual CAP (sprc)

NASA Astrophysics Data System (ADS)

Putri, Alfiah Rizky Diana; Sidiropoulos, Panagiotis; Muller, Jan-Peter

2016-06-01

The surface of Mars has been an object of interest for planetary research since the launch of Mariner 4 in 1964. Since then different cameras such as the Viking Visual Imaging Subsystem (VIS), Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC), and Mars Reconnaissance Orbiter (MRO) Context Camera (CTX) and High Resolution Imaging Science Experiment (HiRISE) have been imaging its surface at ever higher resolution. The High Resolution Stereo Camera (HRSC) on board of the European Space Agency (ESA) Mars Express, has been imaging the Martian surface, since 25th December 2003 until the present-day. HRSC has covered 100 % of the surface of Mars, about 70 % of the surface with panchromatic images at 10-20 m/pixel, and about 98 % at better than 100 m/pixel (Neukum et. al., 2004), including the polar regions of Mars. The Mars polar regions have been studied intensively recently by analysing images taken by the Mars Express and MRO missions (Plaut et al., 2007). The South Polar Residual Cap (SPRC) does not change very much in volume overall but there are numerous examples of dynamic phenomena associated with seasonal changes in the atmosphere. In particular, we can examine the time variation of layers of solid carbon dioxide and water ice with dust deposition (Bibring, 2004), spider-like channels (Piqueux et al., 2003) and so-called Swiss Cheese Terrain (Titus et al., 2004). Because of seasonal changes each Martian year, due to the sublimation and deposition of water and CO2 ice on the Martian south polar region, clearly identifiable surface changes occur in otherwise permanently icy region. In this research, good quality HRSC images of the Mars South Polar region are processed based on previous identification as the optimal coverage of clear surfaces (Campbell et al., 2015). HRSC images of the Martian South Pole are categorized in terms of quality, time, and location to find overlapping areas, processed into high quality Digital Terrain Models (DTMs) and Orthorectified Images (ORIs) and projected into polar stereographic projection using DLR (Deutsches Zentrum für Luft- und Raumfahrt; German Aerospace Center)'s VICAR and GIS software with modifications developed by Kim & Muller (2009). Surface changes are identified in the Mars SPRC region and analysed based on their appearance in the HRSC images.

Gravisensing in single-celled systems

NASA Astrophysics Data System (ADS)

Braun, M.; Limbach, C.

Single-celled systems are favourable cell types for studying several aspects of gravisensing and gravitropic responses. Whether and how actin is involved in both processes in higher plant statocytes is still a matter of intensive debate. In single-celled and tip-growing characean rhizoids and protonemata, however, there is clear evidence that actin is a central keyplayer controlling polarized growth and the mechanisms of gravity sensing and growth reorientation. Both cell types exhibit a unique actin polymerization in the extending tip, strictly colocalized with the prominent ER-aggregate in the center of the Spitzenkoerper. The local accumulation of ADF and profilin in this central array suggest that actin polymerization is controlled by these actin-binding proteins, which can be regulated by calcium, pH and a variety of other parameters. Distinct actin filaments extend even into the outermost tip and form a dense meshwork in the apical and subapical region, before they become bundled by villin to form two populations of thick actin cables that generate rotational cytoplasmic streaming in the basal region. Actomyosin not only mediates the delivery of secretory vesicles to the growing tip and controls the incorporation pattern of cell wall material, but also coordinates the tip-focused distribution pattern of calcium channels in the apical membrane. They establish the tip-high calcium gradient, a prerequisite for exocytosis. Microgravity experiments have added much to our understanding that both cell types use an efficient actomyosin-based system to control and correct the position of their statoliths and to direct sedimenting statoliths to confined graviperception sites at the plasma membrane. Actin's involvement in the graviresponses is more indirect. The upward growth of negatively gravitropic protonemata was shown to be preceded by a statolith-induced relocalization the Ca2+-calcium gradient to the upper flank that does not occur in positively gravitropic (downward growing) rhizoids, in which statoliths sedimentation is followed by differential flank growth. Based on these results, it is evident that polymerization, dynamic reorganization and the diverse functions of actin spatiotemporally controlled by numerous actin-binding proteins are fundamental for the processes of gravity sensing and gravity-oriented polarized growth. Financial support by Deutsches Zentrum für Luft- und Raumfahrt (DLR) on behalf of the Bundesministerium für Bildung und Forschung (50WB9998).

Salmonella meningoencephalomyelitis in a northern fur seal (Callorhinus ursinsus)

USGS Publications Warehouse

Stroud, R.K.; Roelke, M.E.

1980-01-01

Salmonella enteritidis was isolated from the brain of a neonatal northern fur seal (Callorhinus ursinus) with gross and microscopic lesions of meningoencephalomyelitis. Microscopic lesions in the liver and lung suggested septicemia.

Development and Characterization of an Amorphous Solid Dispersion of Furosemide in the Form of a Sublingual Bioadhesive Film to Enhance Bioavailability.

PubMed

De Caro, Viviana; Ajovalasit, Alessia; Sutera, Flavia Maria; Murgia, Denise; Sabatino, Maria Antonietta; Dispenza, Clelia

2017-06-24

Administered by an oral route, Furosemide (FUR), a diuretic used in several edematous states and hypertension, presents bioavailability problems, reported as a consequence of an erratic gastrointestinal absorption due to various existing polymorphic forms and low and pH-dependent solubility. A mucoadhesive sublingual fast-dissolving FUR based film has been developed and evaluated in order to optimize the bioavailability of FUR by increasing solubility and guaranteeing a good dissolution reproducibility. The Differential Scanning Calorimetry (DSC) analyses confirmed that the film prepared using the solvent casting method entrapped FUR in the amorphous state. As a solid dispersion, FUR increases its solubility up to 28.36 mg/mL. Drug content, thickness, and weight uniformity of film were also evaluated. The measured Young's Modulus, yield strength, and relative elongation of break percentage (EB%) allowed for the classification of the drug-loaded film as an elastomer. Mucoadhesive strength tests showed that the force to detach film from mucosa grew exponentially with increasing contact time up to 7667 N/m². FUR was quickly discharged from the film following a trend well fitted with the Weibull kinetic model. When applied on sublingual mucosa, the new formulation produced a massive drug flux in the systemic compartment. Overall, the proposed sublingual film enhances drug solubility and absorption, allowing for the prediction of a rapid onset of action and reproducible bioavailability in its clinical application.

Bacillus subtilis Fur represses one of two paralogous haem-degrading monooxygenases

PubMed Central

Gaballa, Ahmed

2011-01-01

Identification of genes regulated by the ferric uptake regulator (Fur) protein has provided insights into the diverse mechanisms of adaptation to iron limitation. In the soil bacterium Bacillus subtilis, Fur senses iron sufficiency and represses genes that enable iron uptake, including biosynthetic and transport genes for the siderophore bacillibactin and uptake systems for siderophores produced by other organisms. We here demonstrate that Fur regulates hmoA (formerly yetG), which encodes a haem monooxygenase. HmoA is the first characterized member of a divergent group of putative monooxygenases that cluster separately from the well-characterized IsdG family. B. subtilis also encodes an IsdG family protein designated HmoB (formerly YhgC). Unlike hmoA, hmoB is constitutively expressed and not under Fur control. HmoA and HmoB both bind haemin in vitro with approximately 1 : 1 stoichiometry and degrade haemin in the presence of an electron donor. Mutational and spectroscopic analyses indicate that HmoA and HmoB have distinct active site architectures and interact differently with haem. We further show that B. subtilis can use haem as an iron source, but that this ability is independent of HmoA and HmoB. PMID:21873409

Combined evaluation of commonly used techniques, including PCR, for diagnosis of mouse fur mites.

PubMed

Karlsson, Eleanor M; Pearson, Laura M; Kuzma, Kristen M; Burkholder, Tanya H

2014-01-01

Our study evaluated and compared the false-negative rates (FNR) of a wide array of fur-mite diagnostic tests, including 2 postmortem tests (pelt exam and sticky paper) and 3 antemortem tests (adhesive tape, fur pluck, and PCR). Past publications examining fur-mite diagnostic techniques primarily used paired comparisons, evaluating tests by their level of agreement with only one other test. However, different combinations or pairs of diagnostics are used in the different studies, making the results of these comparisons difficult to interpret across all available diagnostics. In the current study, mice from a conventionally maintained colony endemic for Myobia musculi were identified as positive based on at least one positive diagnostic test. From this pool of positive animals, the FNR of all tests were quantified. The PCR assay and the pelt exam performed the best, with 0% and 2% FNR respectively, whereas tape, fur-pluck, and sticky-paper tests showed 24%, 26%, and 36% FNR, respectively. Our study shows that for mice in a colony naturally infested with Myobia musculi, PCR testing can be used for reliable antemortem detection, and pelt exam performed by experienced examiners is reliable for postmortem detection.

SPERM MOTILITY IN HSF1 KNOCKOUT MICE AFTER HEAT SHOCK IS ASSOCIATED WITH FERTILITY DEFICITS

EPA Science Inventory

SPERM MOTILITY IN HSF1 KNOCKOUT MICE AFTER HEAT SHOCK IS ASSOCIATED WITH FERTILITY DEFICITS. L.F. Strader*, S.D. Perreault, J.C. Luft*, and D.J. Dix*. US EPA/ORD, Reproductive Toxicology Div., Research Triangle Park, NC
Heat shock proteins (HSPs) protect cells from environm...

76 FR 27240 - Airworthiness Directives; BURKHART GROB LUFT-UND Model G 103 C Twin III SL Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-11

... AD docket shortly after receipt. List of Subjects in 14 CFR Part 39 Air transportation, Aircraft... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 39 [Docket No. FAA-2011... Administration (FAA), Department of Transportation (DOT). ACTION: Final rule. SUMMARY: We are adopting a new...

DEVELOPMENT OF A 950-GENE DNA ARRAY FOR EXAMINING GENE EXPRESSION PATTERNS IN MOUSE TESTIS

EPA Science Inventory

Development of a 950-gene DNA array for examining gene expression patterns in mouse testis.

Rockett JC, Christopher Luft J, Brian Garges J, Krawetz SA, Hughes MR, Hee Kirn K, Oudes AJ, Dix DJ.

Reproductive Toxicology Division, National Health and Environmental Effec...

Investigations of peritoneal and intestinal infections of adult hookworms (Uncinaria spp.) in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, California (2003).

PubMed

Lyons, Eugene T; Delong, R L; Nadler, S A; Laake, J L; Orr, A J; Delong, B L; Pagan, C

2011-09-01

The peritoneal cavity (PNC) and intestine of northern fur seal (Callorhinus ursinus) pups and California sea lion (Zalophus californianus) pups that died in late July and early August, 2003, on San Miguel Island, California, were examined for hookworms. Prevalence and morphometric studies were done with the hookworms in addition to molecular characterization. Based on this and previous molecular studies, hookworms from fur seals are designated as Uncinaria lucasi and the species from sea lions as Uncinaria species A. Adult hookworms were found in the PNC of 35 of 57 (61.4%) fur seal pups and of 13 of 104 (12.5%) sea lion pups. The number of hookworms located in the PNC ranged from 1 to 33 (median = 3) for the infected fur seal pups and 1 to 16 (median = 2) for the infected sea lion pups. In addition to the PNC, intestines of 43 fur seal and 32 sea lion pups were examined. All of these pups were positive for adult hookworms. The worms were counted from all but one of the sea lion pups. Numbers of these parasites in the intestine varied from 3 to 2,344 (median = 931) for the fur seal pups and 39 to 2,766 (median = 643) for the sea lion pups. Sea lion pups with peritoneal infections had higher intensity infections in the intestines than did pups without peritoneal infections, lending some support for the hypothesis that peritoneal infections result from high-intensity infections of adult worms. There was no difference in intestinal infection intensities between fur seal pups with and without peritoneal infections. Female adult hookworms in the intestines of both host species were significantly larger than males, and sea lion hookworms were larger than those in fur seals. Worms in the intestine also were larger than worms found in the PNC. Gene sequencing and (RFLP) analysis of (PCR) amplified (ITS) ribosomal DNA were used to diagnose the species of 172 hookworms recovered from the PNC and intestine of 18 C. ursinus and seven Z. californianus hosts. These molecular data revealed that U. lucasi (hookworm of C. ursinus) and Uncinaria species A (of Z. californianus) infrequently mature in the intestine of the opposite host species in California rookeries. However, there is no support from molecular data for the hypothesis that cross-infection with "the wrong" Uncinaria species is a contributing factor in these cases of host peritonitis. The major significance of this research is the unusual finding of adult hookworms in the PNC of so many dead pups. No obvious explanation for this occurrence could be determined. Further research, like in the present study, should help understand and monitor the apparent ever changing role of hookworm disease in the health of northern fur seal and California sea lion pups on SMI.

Distinctive Oxidative Stress Responses to Hydrogen Peroxide in Sulfate Reducing Bacteria Desulfovibrio vulgaris Hildenborough

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, Aifen; He, Zhili; Redding, A.M.

2009-01-01

Response of Desulfovibrio vulgaris Hildenborough to hydrogen peroxide (H2O2, 1 mM) was investigated with transcriptomic, proteomic and genetic approaches. Microarray data demonstrated that gene expression was extensively affected by H2O2 with the response peaking at 120 min after H2O2 treatment. Genes affected include those involved with energy production, sulfate reduction, ribosomal structure and translation, H2O2 scavenging, posttranslational modification and DNA repair as evidenced by gene coexpression networks generated via a random matrix-theory based approach. Data from this study support the hypothesis that both PerR and Fur play important roles in H2O2-induced oxidative stress response. First, both PerR and Fur regulonmore » genes were significantly up-regulated. Second, predicted PerR regulon genes ahpC and rbr2 were derepressedin Delta PerR and Delta Fur mutants and induction of neither gene was observed in both Delta PerR and Delta Fur when challenged with peroxide, suggesting possible overlap of these regulons. Third, both Delta PerR and Delta Fur appeared to be more tolerant of H2O2 as measured by optical density. Forth, proteomics data suggested de-repression of Fur during the oxidative stress response. In terms of the intracellular enzymatic H2O2 scavenging, gene expression data suggested that Rdl and Rbr2 may play major roles in the detoxification of H2O2. In addition, induction of thioredoxin reductase and thioredoxin appeared to be independent of PerR and Fur. Considering all data together, D. vulgaris employed a distinctive stress resistance mechanism to defend against increased cellular H2O2, and the temporal gene expression changes were consistent with the slowdown of cell growth at the onset of oxidative stress.« less

Use of flexible ureteroscopy in the clinical practice for the treatment of renal stones: results from a large European survey conducted by the EAU Young Academic Urologists-Working Party on Endourology and Urolithiasis.

PubMed

Sanguedolce, F; Liatsikos, E; Verze, P; Hruby, S; Breda, A; Beatty, J D; Knoll, T

2014-08-01

Treatment of renal stones using flexible ureteroscopy (fURS) is increasingly common despite the poor evidence in literature supporting its use and indications. With this study, we wanted to investigate the current use and indication of fURS for the treatment of renal stones in the clinical practice across the European countries. A survey was conducted using an emailed questionnaire consisting of 21 items; 2,894 recipients were selected via the EAU membership database. The questionnaires were collected through the SurveyMonkey system and the data were processed with the SPSS statistical package. Frequencies, cross tabs and Pearson correlation coefficients were applied as appropriate. 1,168 questionnaires were collected (response rate 40.4%). fURS was performed in 72.9% of the respondents' institutions, and 54.2% of the respondents were performing the procedure. For 95% of the users, fURS was considered first-line treatment, for stone of lower pole stone (45.9%) and <1 cm (44.2%) and 2 cm (43.8%) in size. The ureteral access sheaths were used routinely by more than 70% of the respondents. Lower pole stone repositioning technique was routinely performed by 45.9% of the surgeons. After fragmentation, 47.2% of the responders preferred to retrieve only the bigger fragments. At the end of fURS, lower volume surgeons were more likely to place routinely a double-J stent (p = 0.001). Higher volume surgeons estimated a higher durability of devices, both optical and digital ones (p < 0.001), and were more prone to consider fURS cost-effective when compared to other treatment modalities (p < 0.001). fURS is widely used for the treatment of renal stones and its use and indication can vary according to the age and surgeons' case volume. Higher volume surgeons are more prompt to extend international guidelines indications and to consider the technology cost-effective.

Propeptides are sufficient to regulate organelle-specific pH-dependent activation of furin and proprotein convertase 1/3.

PubMed

Dillon, Stephanie L; Williamson, Danielle M; Elferich, Johannes; Radler, David; Joshi, Rajendra; Thomas, Gary; Shinde, Ujwal

2012-10-12

The proprotein convertases (PCs) furin and proprotein convertase 1/3 (PC1) cleave substrates at dibasic residues along the eukaryotic secretory/endocytic pathway. PCs are evolutionarily related to bacterial subtilisin and are synthesized as zymogens. They contain N-terminal propeptides (PRO) that function as dedicated catalysts that facilitate folding and regulate activation of cognate proteases through multiple-ordered cleavages. Previous studies identified a histidine residue (His69) that functions as a pH sensor in the propeptide of furin (PRO(FUR)), which regulates furin activation at pH~6.5 within the trans-Golgi network. Although this residue is conserved in the PC1 propeptide (PRO(PC1)), PC1 nonetheless activates at pH~5.5 within the dense core secretory granules. Here, we analyze the mechanism by which PRO(FUR) regulates furin activation and examine why PRO(FUR) and PRO(PC1) differ in their pH-dependent activation. Sequence analyses establish that while both PRO(FUR) and PRO(PC1) are enriched in histidines when compared with cognate catalytic domains and prokaryotic orthologs, histidine content in PRO(FUR) is ~2-fold greater than that in PRO(PC1), which may augment its pH sensitivity. Spectroscopy and molecular dynamics establish that histidine protonation significantly unfolds PRO(FUR) when compared to PRO(PC1) to enhance autoproteolysis. We further demonstrate that PRO(FUR) and PRO(PC1) are sufficient to confer organelle sensing on folding and activation of their cognate proteases. Swapping propeptides between furin and PC1 transfers pH-dependent protease activation in a propeptide-dictated manner in vitro and in cells. Since prokaryotes lack organelles and eukaryotic PCs evolved from propeptide-dependent, not propeptide-independent prokaryotic subtilases, our results suggest that histidine enrichment may have enabled propeptides to evolve to exploit pH gradients to activate within specific organelles. Copyright © 2012 Elsevier Ltd. All rights reserved.

Comparison of the efficacy and morbidity of flexible ureterorenoscopy for lower pole stones compared with other renal locations.

PubMed

Jacquemet, Baptiste; Martin, Lucille; Pastori, Julie; Bailly, Vincent; Guichard, Guillaume; Bernardini, Stéphane; Chabannes, Eric; Bittard, Hugues; Kleinclauss, François

2014-10-01

Flexible ureterorenoscopy (f-URS) for lower pole stones (LPS) compared with other renal locations can be challenging because of anatomic and technical considerations. We aimed to compare the stone-free rate (SFR) and surgical complication rate with f-URS for LPS vs other renal locations. We performed a retrospective, single-center study including 371 f-URS for renal stone retrieval performed in our institution between January 2004 and December 2010. Among the 371 procedures included in this analysis, 139 were performed for stones located in a single renal location other than the lower pole (group 1), and 232 for at least one stone located in the lower pole (group 2). We compared the efficacy (SFR) and the morbidity of f-URS between the two groups. The success of the procedure was defined as a complete SFR 6 months after f-URS. Age, sex, history of urolithiasis, body mass index, and preoperative stent placement did not differ between the two groups. No differences in stone characteristics were observed between both groups except stone size under 10 mm that was significantly higher in group 2 (P=0.018). Technical aspects of the procedure did not differ between the groups, except for more frequent use of an access sheath in group 2 (P=0.007). SFR was comparable between groups (P=0.774). The complication rate was similar in both groups, as was the severity of complications. By multivariate analysis, stone size >10 mm (P<0.0001) and multiple stone locations (P=0.001) were associated with f-URS failure, but lower pole location did not impact on SFR. In our study, stone location, in particular LPS, did not have any impact on efficacy and morbidity of f-URS. Only multiple locations and stone size >10 mm seemed to significantly decrease the SFR, without impacting morbidity.

Current prevalence of adult Uncinaria spp. in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, California, with notes on the biology of these hookworms.

PubMed

Lyons, E T; Melin, S R; DeLong, R L; Orr, A J; Gulland, F M; Tolliver, S C

2001-06-28

A prevalence survey for hookworms (Uncinaria spp.) was done in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, CA, in 2000. Intestines of dead pups were examined for adult hookworms in July. These parasites were found in 95% of 20 fur seal pups and 100% of 31 sea lion pups. The number of hookworms varied from 4 to 2142 (mean = 760) in fur seal pups and from 20 to 2634 (mean = 612) in sea lion pups. A direct relationship was evident between body condition and number of hookworms in the pups; that is, pups in poor condition had fewer hookworms than those in good condition. There was a decline in the number of hookworms in sea lion pups in 2000 compared to collections in 1996. Eggs of Uncinaria spp. were found in rectal feces (collected in late September and early October) of none of 35 (0%) live fur seal pups and 41 of 48 (85%) live sea lion pups. Packed cell volume values, determined for most of the same live pups, were essentially normal for C. ursinus but were much lower than normal for most Z. californianus. Hookworm larvae were not found in blubber of fur seal and sea lion pups or in rookery sand in July. Rookery sand, positive for live hookworm larvae when put in a refrigerator, was negative at removal 2.5 years later. The average number of eggs in utero of female hookworms was 285 for three specimens from a fur seal pup and 281 from three specimens from a sea lion pup. One hookworm larva was recovered from milk stripped from the teats of a stranded Z. californianus female at The Marine Mammal Center, Sausalito, CA.

Spatial variation of mercury bioaccumulation in bats of Canada linked to atmospheric mercury deposition.

PubMed

Chételat, John; Hickey, M Brian C; Poulain, Alexandre J; Dastoor, Ashu; Ryjkov, Andrei; McAlpine, Donald; Vanderwolf, Karen; Jung, Thomas S; Hale, Lesley; Cooke, Emma L L; Hobson, Dave; Jonasson, Kristin; Kaupas, Laura; McCarthy, Sara; McClelland, Christine; Morningstar, Derek; Norquay, Kaleigh J O; Novy, Richard; Player, Delanie; Redford, Tony; Simard, Anouk; Stamler, Samantha; Webber, Quinn M R; Yumvihoze, Emmanuel; Zanuttig, Michelle

2018-06-01

Wildlife are exposed to neurotoxic mercury at locations distant from anthropogenic emission sources because of long-range atmospheric transport of this metal. In this study, mercury bioaccumulation in insectivorous bat species (Mammalia: Chiroptera) was investigated on a broad geographic scale in Canada. Fur was analyzed (n=1178) for total mercury from 43 locations spanning 20° latitude and 77° longitude. Total mercury and methylmercury concentrations in fur were positively correlated with concentrations in internal tissues (brain, liver, kidney) for a small subset (n=21) of little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus), validating the use of fur to indicate internal mercury exposure. Brain methylmercury concentrations were approximately 10% of total mercury concentrations in fur. Three bat species were mainly collected (little brown bats, big brown bats, and northern long-eared bats [M. septentrionalis]), with little brown bats having lower total mercury concentrations in their fur than the other two species at sites where both species were sampled. On average, juvenile bats had lower total mercury concentrations than adults but no differences were found between males and females of a species. Combining our dataset with previously published data for eastern Canada, median total mercury concentrations in fur of little brown bats ranged from 0.88-12.78μg/g among 11 provinces and territories. Highest concentrations were found in eastern Canada where bats are most endangered from introduced disease. Model estimates of atmospheric mercury deposition indicated that eastern Canada was exposed to greater mercury deposition than central and western sites. Further, mean total mercury concentrations in fur of adult little brown bats were positively correlated with site-specific estimates of atmospheric mercury deposition. This study provides the largest geographic coverage of mercury measurements in bats to date and indicates that atmospheric mercury deposition is important in determining spatial patterns of mercury accumulation in a mammalian species. Crown Copyright © 2018. Published by Elsevier B.V. All rights reserved.

A colostrum trypsin inhibitor gene expressed in the Cape fur seal mammary gland during lactation.

PubMed

Pharo, Elizabeth A; Cane, Kylie N; McCoey, Julia; Buckle, Ashley M; Oosthuizen, W H; Guinet, Christophe; Arnould, John P Y

2016-03-01

The colostrum trypsin inhibitor (CTI) gene and transcript were cloned from the Cape fur seal mammary gland and CTI identified by in silico analysis of the Pacific walrus and polar bear genomes (Order Carnivora), and in marine and terrestrial mammals of the Orders Cetartiodactyla (yak, whales, camel) and Perissodactyla (white rhinoceros). Unexpectedly, Weddell seal CTI was predicted to be a pseudogene. Cape fur seal CTI was expressed in the mammary gland of a pregnant multiparous seal, but not in a seal in its first pregnancy. While bovine CTI is expressed for 24-48 h postpartum (pp) and secreted in colostrum only, Cape fur seal CTI was detected for at least 2-3 months pp while the mother was suckling its young on-shore. Furthermore, CTI was expressed in the mammary gland of only one of the lactating seals that was foraging at-sea. The expression of β-casein (CSN2) and β-lactoglobulin II (LGB2), but not CTI in the second lactating seal foraging at-sea suggested that CTI may be intermittently expressed during lactation. Cape fur seal and walrus CTI encode putative small, secreted, N-glycosylated proteins with a single Kunitz/bovine pancreatic trypsin inhibitor (BPTI) domain indicative of serine protease inhibition. Mature Cape fur seal CTI shares 92% sequence identity with Pacific walrus CTI, but only 35% identity with BPTI. Structural homology modelling of Cape fur seal CTI and Pacific walrus trypsin based on the model of the second Kunitz domain of human tissue factor pathway inhibitor (TFPI) and porcine trypsin (Protein Data Bank: 1TFX) confirmed that CTI inhibits trypsin in a canonical fashion. Therefore, pinniped CTI may be critical for preventing the proteolytic degradation of immunoglobulins that are passively transferred from mother to young via colostrum and milk. Copyright © 2015 Elsevier B.V. All rights reserved.

29 CFR 780.119 - Employment in the specified operations generally.

Code of Federal Regulations, 2011 CFR

2011-07-01

... STANDARDS ACT General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780... livestock, bees, fur-bearing animals or poultry only if their operations relate to animals of the type named...

29 CFR 780.119 - Employment in the specified operations generally.

Code of Federal Regulations, 2010 CFR

2010-07-01

... STANDARDS ACT General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780... livestock, bees, fur-bearing animals or poultry only if their operations relate to animals of the type named...

29 CFR 780.119 - Employment in the specified operations generally.

Code of Federal Regulations, 2014 CFR

2014-07-01

... STANDARDS ACT General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780... livestock, bees, fur-bearing animals or poultry only if their operations relate to animals of the type named...

29 CFR 780.119 - Employment in the specified operations generally.

Code of Federal Regulations, 2012 CFR

2012-07-01

... STANDARDS ACT General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780... livestock, bees, fur-bearing animals or poultry only if their operations relate to animals of the type named...

29 CFR 780.119 - Employment in the specified operations generally.

Code of Federal Regulations, 2013 CFR

2013-07-01

... STANDARDS ACT General Scope of Agriculture Raising of Livestock, Bees, Fur-Bearing Animals, Or Poultry § 780... livestock, bees, fur-bearing animals or poultry only if their operations relate to animals of the type named...

16 CFR 301.38 - Advertising of furs and fur products.

Code of Federal Regulations, 2010 CFR

2010-01-01

... general nature or kind of business conducted or to the general classification of the types or kinds of... Dyed Persian Lamb Since 1900 or X Company Manufacturers of Fine Muskrat Coats, Capes and Stoles ...

19 CFR 12.62 - Enforcement; duties of Customs officers.

Code of Federal Regulations, 2014 CFR

2014-04-01

...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.62 Enforcement... made pursuant thereto; and shall seize fur seals and sea otters, or the skins thereof, killed, captured...

19 CFR 12.62 - Enforcement; duties of Customs officers.

Code of Federal Regulations, 2011 CFR

2011-04-01

...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.62 Enforcement... made pursuant thereto; and shall seize fur seals and sea otters, or the skins thereof, killed, captured...

19 CFR 12.62 - Enforcement; duties of Customs officers.

Code of Federal Regulations, 2012 CFR

2012-04-01

...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.62 Enforcement... made pursuant thereto; and shall seize fur seals and sea otters, or the skins thereof, killed, captured...

19 CFR 12.62 - Enforcement; duties of Customs officers.

Code of Federal Regulations, 2013 CFR

2013-04-01

...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.62 Enforcement... made pursuant thereto; and shall seize fur seals and sea otters, or the skins thereof, killed, captured...

19 CFR 12.62 - Enforcement; duties of Customs officers.

Code of Federal Regulations, 2010 CFR

2010-04-01

...; DEPARTMENT OF THE TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.62 Enforcement... made pursuant thereto; and shall seize fur seals and sea otters, or the skins thereof, killed, captured...

Mercury in fur of Daubenton's bat (Myotis daubentonii) in Southern Sweden and Comparison to Ecotoxicological Thresholds.

PubMed

Åkerblom, Staffan; de Jong, Johnny

2017-11-01

To characterise mercury (Hg) exposure in Daubenton's bat (Myotis daubentonii, Kuhl 1817) in southern Sweden, 17 specimens were captured in 2013 and back fur samples were taken for analysis to determine Hg concentrations. The fur Hg levels determined [1.15 ± 0.27 (mean ± standard deviation, n = 17) µg Hg g -1 fresh weight (fw)] represent a baseline for comparison in future assessments of Hg exposure in bat populations in northern Europe. Mercury concentrations were close to those reported in fur from other bat species, but were lower than proposed toxicological thresholds in bats (> 30 µg Hg g -1  fw) and mice (5 µg Hg g -1  fw). This is the first study to examine Hg exposure in bats in Scandinavia.

Uptake of selenium and mercury by captive mink: Results of a controlled feeding experiment.

PubMed

Evans, R D; Grochowina, N M; Basu, N; O'Connor, E M; Hickie, B E; Rouvinen-Watt, K; Evans, H E; Chan, H M

2016-02-01

Captive, juvenile, ranch-bred, male mink (Neovison vison) were fed diets containing various concentrations of methyl-mercury (MeHg) and selenium (Se) for a period of 13 weeks and then sacrificed to determine total Hg levels in fur, blood, brain, liver and kidneys and total Se concentrations in brain tissue. As MeHg concentrations in the diet increased, concentrations of total Hg in the tissues also increased with the highest level occurring in the fur > liver = kidney > brain > blood. Concentrations of Hg in the fur were correlated (r(2) > 0.97) with liver, kidney, blood and brain concentrations. The addition of Se to the mink diet did not appear to affect most tissue concentrations of total Hg nor did it affect the partitioning of Hg between the liver:blood, kidney:blood and brain:blood; however, partitioning of Hg between fur and blood was apparently affected. Copyright © 2015 Elsevier Ltd. All rights reserved.

Technology Solutions Case Study: Duct in Conditioned Space in a Dropped Ceiling or Fur-down, Gainesville, Florida

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2014-09-01

Forced-air distribution systems (duct systems) typically are installed out of sight for aesthetic reasons, most often in unconditioned areas such as attics or crawlspaces. Any leakage of air to or from the duct system in unconditioned space not only loses energy, but impacts home and equipment durability and indoor air quality. An obvious solution is to bring the duct system into the interior of the house, either by sealing the area where the ducts are installed (attic or crawlspace) or by building an interior cavity or chase above the ceiling plane (raised ceiling or fur-up chase) or below the ceilingmore » plane (dropped ceiling or fur-down) for the duct system. In this project, Building America Partnership for Improved Residential Construction team partnered with Tommy Williams Homes to implement an inexpensive, quick, and effective method of building a fur-down chase.« less

Flexible ureteroscopy versus laparoscopy for the treatment of patients who initially presented with obstructive pyelonephritis

PubMed Central

Sahin, Selcuk; Resorlu, Berkan; Eksi, Mithat; Aras, Bekir; Atar, Arda; Tugcu, Volkan

2016-01-01

Objective: To compare the safety and effectiveness of flexible ureteroscopy (F-URS) with transperitoneal laparoscopic ureterolithotomy (TPLU) in cases of obstructive pyelonephritis secondary to large proximal ureteral stones. Methods: A series of 42 patients presenting with obstructive pyelonephritis due to proximal ureteral stones larger than 1.5 cm were included from April 2006 to February 2015 in this comparative study. After drainage of pyonephrosis and resolution of sepsis, 22 patients treated with TPLU (Group I), and 20 patients were treated with F-URS (Group II). Preoperative patient and stone characteristics, procedure-related parameters and clinical outcomes were assessed for each group. Results: It was seen that both methods were effective in the treatment of large proximal ureteral stones. However TPLU provided a higher stone- free rate (100% vs 80%. p=0.043) and lower retreatment rate. There was no difference between the groups for the operative time and complication rate. On the other hand, patients treated with F-URS had less postoperative pain (p=0.008), a shorter hospital stay (p<0.001) and a faster return to daily activities (p<0.001). Conclusions: The results of our study show that both F-URS and TPLU are safe and effective surgical procedures for treatment of large proximal ureteral stones after controlling obstructive pyelonephritis. However, TPLU has a higher stone-free rate with comparable operating time and complication rate as compared to F-URS. On the other hand F-URS has the advantages of less postoperative pain, shorter hospital stay and faster return to daily activities. PMID:27375691

50 CFR 223.201 - Guadalupe fur seal.

Code of Federal Regulations, 2010 CFR

2010-10-01

....201 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Restrictions Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The...

50 CFR 223.201 - Guadalupe fur seal.

Code of Federal Regulations, 2012 CFR

2012-10-01

....201 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Restrictions Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The...

50 CFR 223.201 - Guadalupe fur seal.

Code of Federal Regulations, 2013 CFR

2013-10-01

....201 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Restrictions Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The...

50 CFR 223.201 - Guadalupe fur seal.

Code of Federal Regulations, 2011 CFR

2011-10-01

....201 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Restrictions Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The...

50 CFR 223.201 - Guadalupe fur seal.

Code of Federal Regulations, 2014 CFR

2014-10-01

....201 Wildlife and Fisheries NATIONAL MARINE FISHERIES SERVICE, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE MARINE MAMMALS THREATENED MARINE AND ANADROMOUS SPECIES Restrictions Applicable to Threatened Marine and Anadromous Species § 223.201 Guadalupe fur seal. (a) Prohibitions. The...

A decrease in heat insulation of the black-clawed brush-furred rat (Lophuromys melanonyx, Petter) during adaptation to high altitudes.

PubMed

Ivlev, Y F; Lavrenchenko, L A

2016-01-01

The results of the body-surface infrared thermography of rodents of the genus Lophuromys suggest that heat insulation of the black-clawed brush-furred rat L. melanonyx, a large specialized species of the AfroAlpine zone, is worse than that of the related smaller species, the golden-footed (L. chrysopus) and shorttailed (L. brevicaudus) brush-furred rats, that inhabit tropical forest and Erica shrub, respectively. A decrease in heat insulation of the alpine species may facilitate the use of solar radiation for supporting heat balance of these diurnal animals.

The Fur-Iron Complex Modulates Expression of the Quorum-Sensing Master Regulator, SmcR, To Control Expression of Virulence Factors in Vibrio vulnificus

PubMed Central

Kim, In Hwang; Wen, Yancheng; Son, Jee-Soo; Lee, Kyu-Ho

2013-01-01

The gene vvpE, encoding the virulence factor elastase, is a member of the quorum-sensing regulon in Vibrio vulnificus and displays enhanced expression at high cell density. We observed that this gene was repressed under iron-rich conditions and that the repression was due to a Fur (ferric uptake regulator)-dependent repression of smcR, a gene encoding a quorum-sensing master regulator with similarity to luxR in Vibrio harveyi. A gel mobility shift assay and a footprinting experiment demonstrated that the Fur-iron complex binds directly to two regions upstream of smcR (−82 to −36 and −2 to +27, with respect to the transcription start site) with differing affinities. However, binding of the Fur-iron complex is reversible enough to allow expression of smcR to be induced by quorum sensing at high cell density under iron-rich conditions. Under iron-limiting conditions, Fur fails to bind either region and the expression of smcR is regulated solely by quorum sensing. These results suggest that two biologically important environmental signals, iron and quorum sensing, converge to direct the expression of smcR, which then coordinates the expression of virulence factors. PMID:23716618

Mercury contamination in bats from the central United States.

PubMed

Korstian, Jennifer M; Chumchal, Matthew M; Bennett, Victoria J; Hale, Amanda M

2018-01-01

Mercury (Hg) is a highly toxic metal that has detrimental effects on wildlife. We surveyed Hg concentrations in 10 species of bats collected at wind farms in the central United States and found contamination in all species. Mercury concentration in fur was highly variable both within and between species (range: 1.08-10.52 µg/g). Despite the distance between sites (up to 1200 km), only 2 of the 5 species sampled at multiple locations had fur Hg concentrations that differed between sites. Mercury concentrations observed in the present study all fell within the previously reported ranges for bats collected from the northeastern United States and Canada, although many of the bats we sampled had lower maximum Hg concentrations. Juvenile bats had lower concentrations of Hg in fur compared with adult bats, and we found no significant effect of sex on Hg concentrations in fur. For a subset of 2 species, we also measured Hg concentration in muscle tissue; concentrations were much higher in fur than in muscle, and Hg concentrations in the 2 tissue types were weakly correlated. Abundant wind farms and ongoing postconstruction fatality surveys offer an underutilized opportunity to obtain tissue samples that can be used to assess Hg contamination in bats. Environ Toxicol Chem 2018;37:160-165. © 2018 SETAC. © 2017 SETAC.

Appropriate kidney stone size for ureteroscopic lithotripsy: When to switch to a percutaneous approach

PubMed Central

Takazawa, Ryoji; Kitayama, Sachi; Tsujii, Toshihiko

2015-01-01

Flexible ureteroscopy (fURS) has become a more effective and safer treatment for whole upper urinary tract stones. Percutaneous nephrolithotomy (PNL) is currently the first-line recommended treatment for large kidney stones ≥ 20 mm and it has an excellent stone-free rate for large kidney stones. However, its invasiveness is not negligible considering its major complication rates. Staged fURS is a practical treatment for such large kidney stones because fURS has a minimal blood transfusion risk, short hospitalization and few restrictions on daily routines. However, as the stone size becomes larger, the stone-free rate decreases, and the number of operations required increases. Therefore, in our opinion, staged fURS is a practical option for kidney stones 20 to 40 mm. Miniaturized PNL combined with fURS should be considered to be a preferred option for stones larger than 40 mm. Moreover, URS is an effective treatment for multiple upper urinary tract stones. Especially for patients with a stone burden < 20 mm, URS is a favorable option that promises a high stone-free rate after a single session either unilaterally or bilaterally. However, for patients with a stone burden ≥ 20 mm, a staged operation should be considered to achieve stone-free status. PMID:25664253

Genetic Diversity and Population Parameters of Sea Otters, Enhydra lutris, before Fur Trade Extirpation from 1741–1911

PubMed Central

Larson, Shawn; Jameson, Ron; Etnier, Michael; Jones, Terry; Hall, Roberta

2012-01-01

All existing sea otter, Enhydra lutris, populations have suffered at least one historic population bottleneck stemming from the fur trade extirpations of the eighteenth and nineteenth centuries. We examined genetic variation, gene flow, and population structure at five microsatellite loci in samples from five pre-fur trade populations throughout the sea otter's historical range: California, Oregon, Washington, Alaska, and Russia. We then compared those values to genetic diversity and population structure found within five modern sea otter populations throughout their current range: California, Prince William Sound, Amchitka Island, Southeast Alaska and Washington. We found twice the genetic diversity in the pre-fur trade populations when compared to modern sea otters, a level of diversity that was similar to levels that are found in other mammal populations that have not experienced population bottlenecks. Even with the significant loss in genetic diversity modern sea otters have retained historical structure. There was greater gene flow before extirpation than that found among modern sea otter populations but the difference was not statistically significant. The most dramatic effect of pre fur trade population extirpation was the loss of genetic diversity. For long term conservation of these populations increasing gene flow and the maintenance of remnant genetic diversity should be encouraged. PMID:22403635

Symmetry based assembly of a 2 dimensional protein lattice

DOE Office of Scientific and Technical Information (OSTI.GOV)

Poulos, Sandra; Agah, Sayeh; Jallah, Nikardi

2017-04-18

The design of proteins that self-assemble into higher order architectures is of great interest due to their potential application in nanotechnology. Specifically, the self-assembly of proteins into ordered lattices is of special interest to the field of structural biology. Here we designed a 2 dimensional (2D) protein lattice using a fusion of a tandem repeat of three TelSAM domains (TTT) to the Ferric uptake regulator (FUR) domain. We determined the structure of the designed (TTT-FUR) fusion protein to 2.3 Å by X-ray crystallographic methods. In agreement with the design, a 2D lattice composed of TelSAM fibers interdigitated by the FURmore » domain was observed. As expected, the fusion of a tandem repeat of three TelSAM domains formed 21 screw axis, and the self-assembly of the ordered oligomer was under pH control. We demonstrated that the fusion of TTT to a domain having a 2-fold symmetry, such as the FUR domain, can produce an ordered 2D lattice. The TTT-FUR system combines features from the rotational symmetry matching approach with the oligomer driven crystallization method. This TTT-FUR fusion was amenable to X-ray crystallographic methods, and is a promising crystallization chaperone.« less

A syndrome of mutualism reinforces the lifestyle of a sloth

PubMed Central

Pauli, Jonathan N.; Mendoza, Jorge E.; Steffan, Shawn A.; Carey, Cayelan C.; Weimer, Paul J.; Peery, M. Zachariah

2014-01-01

Arboreal herbivory is rare among mammals. The few species with this lifestyle possess unique adaptions to overcome size-related constraints on nutritional energetics. Sloths are folivores that spend most of their time resting or eating in the forest canopy. A three-toed sloth will, however, descend its tree weekly to defecate, which is risky, energetically costly and, until now, inexplicable. We hypothesized that this behaviour sustains an ecosystem in the fur of sloths, which confers cryptic nutritional benefits to sloths. We found that the more specialized three-toed sloths harboured more phoretic moths, greater concentrations of inorganic nitrogen and higher algal biomass than the generalist two-toed sloths. Moth density was positively related to inorganic nitrogen concentration and algal biomass in the fur. We discovered that sloths consumed algae from their fur, which was highly digestible and lipid-rich. By descending a tree to defecate, sloths transport moths to their oviposition sites in sloth dung, which facilitates moth colonization of sloth fur. Moths are portals for nutrients, increasing nitrogen levels in sloth fur, which fuels algal growth. Sloths consume these algae-gardens, presumably to augment their limited diet. These linked mutualisms between moths, sloths and algae appear to aid the sloth in overcoming a highly constrained lifestyle. PMID:24452028

75 FR 31734 - Airworthiness Directives; GROB-WERKE (Type Certificate Previously Held by BURKHART GROB Luft- und...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-04

... manufacturer has received a report of a failed canopy jettison test, during a regular maintenance check. The... jettison test, during a regular maintenance check. The investigation revealed that a cable shroud of the... Information Grob Aircraft AG has issued Service Bulletin No. MSB1078-164, dated July 21, 2009. The actions...

75 FR 12466 - Airworthiness Directives; GROB-WERKE (Type Certificate Previously Held by BURKHART GROB Luft- und...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-16

... failed canopy jettison test, during a regular maintenance check. The investigation revealed that a cable... received by the closing date and may amend this proposed AD because of those comments. We will post all... provide. We will also post a report summarizing each substantive verbal contact we receive about this...

THE EFFECTS OF HYPERTHERMIA ON SPERMATOGENESIS, APOPTOSIS, GENE EXPRESSION AND FERTILITY IN ADULT MALE MICE

EPA Science Inventory

The effects of hyperthermia on spermatogenesis, apoptosis, gene expression and fertility in adult male mice
John C. Rockett1, Faye L. Mapp1, J. Brian Garges1, J. Christopher Luft1, Chisato Mori2 and David J. Dix1.
1Reproductive Toxicology Division, National Health and Envir...

Service Provider Perceptions of Key Factors Related to Postsecondary Success of Students with Learning Disabilities in Institutions of Higher Education

ERIC Educational Resources Information Center

Herring, Nathan L.

2012-01-01

Students with learning disabilities have been attending postsecondary education in increasing numbers since the early 1990's. Improvements in secondary education services, legal rights to reasonable accommodations, and college services for these students have been credited with this increase (Browning, 1997; Flexer, Simmons, Luft, & Baer,…

REPRODUCTIVE EFFECTS OF THE WATER DISINFECTANT BYPRODUCT BROMOCHLOROACETIC ACID (BCA) IN ADULT AND JUVENILE MALE C57BL/6 MICE

EPA Science Inventory

REPRODUCTIVE EFFECTS OF THE WATER DISINFECTANT BYPRODUCT BROMOCHLOROACETIC ACID (BCA) IN ADULT AND JUVENILE MALE C57BL/6 MICE.
JC Rockett, JC Luft, JB Garges and DJ Dix. Reproductive Toxicology Division, USEPA, RTP, NC, USA.
Sponsor: G Klinefelter
The development of wate...

USING DNA MICROARRAYS TO CHARACTERIZE GENE EXPRESSION IN TESTES OF FERTILE AND INFERTILE HUMANS AND MICE

EPA Science Inventory

USING DNA MICROARRAYS TO CHARACTERIZE GENE EXPRESSION
IN TESTES OF FERTILE AND INFERTILE HUMANS AND MICE

John C. Rockett1, J. Christopher Luft1, J. Brian Garges1, M. Stacey Ricci2, Pasquale Patrizio2, Norman B. Hecht2 and David J. Dix1
Reproductive Toxicology Divisio...

The German Interlinguistics Society Gesellschaft fur Interlinguistik.

ERIC Educational Resources Information Center

O Riain, Sean

2003-01-01

Describes the German interlinguistics society Gesellschaft fur Interlinguistik (GIL), which was founded to bring together interlinguistics and esperantology scholars. Highlights GIL's principal fields of activity and discusses its role in the fields of international linguistic communication, language planning, esperantolgy, and the teaching of…

Comparison of miniaturized percutaneous nephrolithotomy and flexible ureterorenoscopy for moderate size renal stones in elderly patients.

PubMed

Ozgor, Faruk; Yanaral, Fatih; Savun, Metin; Ozdemir, Harun; Caglar, Ufuk; Sarilar, Omer

2018-06-01

Life expectancy has become longer, thus the number of elderly people who require treatment for nephrolithiasis has increased. We aimed to analyze the efficacy of flexible ureterorenoscopy (f-URS) and miniaturized percutaneous nephrolithotomy (mPNL) in the management of 10 and 30 mm renal stones in patients aged >60 years. In prospective non-randomized series, the data of patients who underwent f-URS or mPNL for kidney stones between July 2013 and July 2016 were analyzed. The procedure was accepted as successful if the patient was achieved complete stone clearance according to CT imaging between 1-3 months postoperatively. In total 60 patients and 58 patients were underwent f-URS and mPNL, respectively. The mean operation time, fluoroscopy time and hospitalization time were significantly shorter for the f-URS (p < 0.001, p < 0.001, p < 0.001, respectively). According to Clavien classification system, complication rates were not significantly different between the groups (p = 0.673). The stone-free rate was 81.7% for the f-URS group and 77.6% for the mPNL group after a single-session procedure (p = 0.747). Calcium oxalate monohydrate stones were the most common stone type in both groups. In multivariate analysis, multiple stones localization was only independent factor to predict complications. Our study had showed that both f-URS and mPNL are effective treatment modalities for 10-30-mm renal stones in elderly patients. Additionally, presence of stones in multiple location was the only predictive factor for complication development. Copyright © 2017 Kaohsiung Medical University. Published by Elsevier Taiwan. All rights reserved.

Epizoochorous dispersal by ungulates depends on fur, grooming and social interactions.

PubMed

Liehrmann, Océane; Jégoux, Flore; Guilbert, Marie-Alice; Isselin-Nondedeu, Francis; Saïd, Sonia; Locatelli, Yann; Baltzinger, Christophe

2018-02-01

The transport phase of the animal-mediated plant dispersal process is critical to dispersal effectiveness as it determines the spatial distribution of the diaspores released and their chance for further recruitment. Assessing this specific phase of the dispersal process generally requires combining diaspore retention times with the associated distances covered. Here, we specifically tested the effect of grooming behavior, interindividual contacts and ungulate fur on diaspore retention times and associated dispersal distances for the hooked diaspores of Xanthium strumarium L. experimentally attached to tamed individuals of three ungulate species. We used a comparative approach based on differing fur quality on different body zones of these three ungulates. During 6-hr sessions, we monitored for grooming and social interactions that may induce intended or inadvertent diaspore detachment. Additionally, we proposed innovative approaches to directly assessing diaspore dispersal distances by red deer in situ. Fat-tailed functions fitted diaspore retention time, highlighting the potential for long-distance dispersal events. The longer the hair, the higher the retention capacity of diaspores in the animal's fur. As predicted, donkey retained diaspores longer than red deer and dwarf goat; and we also confirmed that diaspores attached to the short hair of the head fell off more quickly than did those on the other body zones. Dwarf goat groomed more often than both red deer and donkey, but also when it carried diaspores. Up to 14% of the diaspores detached from animal fur after specific grooming behavior. We observed, in controlled conditions, for the first time and for each ungulate species, interindividual transfers of diaspores, representing 5% of the diaspores attached to animals' fur. Our results militate for incorporating animal behavior into plant dispersal modeling approaches.

Historic cohort study in Montreal's fur industry.

PubMed

Guay, D; Siemiatycki, J

1987-01-01

A historic cohort mortality study was carried out among two groups of male workers in the Montreal fur industry: 263 dressers and dyers and 599 fur garment manufacturers. The first group is exposed to a very wide variety of chemicals used in tanning, cleaning, and dyeing fur, including substances considered to be carcinogenic and/or mutagenic. The second group is exposed to residue from the dressing and dyeing stage and to respirable fur dust. The cohorts consisted of all active members of two unions as of January 1, 1966. The mean age of the workers was 43.2 and the mean number of years since first employment 14.1. The follow-up period was from January 1, 1966, to December 31, 1981; 95% of the workers were successfully traced. Observed deaths were compared with those expected based on mortality rates of the population of metropolitan Montreal. Standardized mortality ratios (SMRs) for the manufacturers were significantly low, probably because of the ethnic composition of the cohort and a healthy worker effect. SMRs for the dressers and dyers were also low, but not as low as for the manufacturers. When attention was restricted to the French Canadians in the cohort, the observed deaths were close to the expected; there was a noteworthy excess of colorectal cancer (four observed, 0.8 expected) for dressers and dyers. Apart from this weak suggestive evidence, the results did not indicate any excess mortality risks in the fur industry. However, because of the relatively small number of expected and observed deaths in the cohort and especially among the heavily exposed dressers and dyers, the confidence intervals around SMR estimates were wide and excess risks cannot be ruled out.

Uptake mechanism of furosemide-loaded pegylated nanoparticles by cochlear cell lines.

PubMed

Youm, Ibrahima; Youan, Bi-Botti C

2013-10-01

This study tests the hypothesis that pegylated nanoparticles (NPs) could be taken up by the cochlear cells [House Ear Institute-organ of Corti 1 (HEI-OC1) and Stria vascularis K-1 (SVK-1)], through endocytic pathways. Furthermore, the in vitro drug release and the cytotoxicity of Furosemide (FUR)-loaded NPs on these two cochlear cells are investigated. FUR-loaded pegylated NPs are prepared by the emulsion-solvent diffusion method without surfactant. The NPs are characterized for particle mean diameter, polydispersity index (PDI), morphology, percent drug encapsulation efficiency (EE%), and FUR release kinetics. The methyl tetrazolium salt (MTS) and lactate dehydrogenase (LDH) bioassays are used to evaluate in vitro, the cytotoxicity of FUR-loaded NPs and native FUR. The NPs uptake is investigated using confocal microscopy, microplate reader/fluorimetry, and flow cytometry. Spherical NPs with a mean diameter range of 133-210 nm and PDI values varying from 0.037 to 0.41 are produced. The FUR EE% is 86% and the drug is released from the NPs according to the zero-order and Higuchi models. After treatment with blank NPs, the percentage of cell viability and cell death are 95.96% and 8.95%, in HEI-OC1 cells, respectively. The NPs are internalized by HEI-OC1 cells through a clathrin-dependent pathway. In addition, results show that NPs can be taken up via clathrin and cytoskeleton mediated pathways in SVK-1 cells. The internalization of the pegylated NPs can enhance the drug toxicity by necrosis in a dose-dependent and sustained release manner. The formulated NPs provide a promising template for a targeted drug delivery system to the inner ear. Copyright © 2013 Elsevier B.V. All rights reserved.

From the Fur Trade to Acid Rain: A Study of Canadian Natural Resources.

ERIC Educational Resources Information Center

Winans, Linda

1988-01-01

Presents a teaching module for upper elementary students that devotes eight class periods of study to Canadian resources. Includes study of the Canadian fur trade, fishing industry, forestry, and the problems caused by acid rain. Includes the unit evaluation. (DB)

Mercury concentrations in wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada: relationship to age and parasitism.

PubMed

Klenavic, Katherine; Champoux, Louise; Mike, O'Brien; Daoust, Pierre-Y; Evans, R Douglas; Evans, Hayla E

2008-11-01

Total mercury (Hg) concentrations were measured in the fur, brain and liver of wild mink (Mustela vison) and river otters (Lontra canadensis) collected from eastern and Atlantic Canada. Total Hg concentrations in fur were strongly correlated with levels in the brain and liver. There was no difference in tissue concentrations between male and female mink; however, female otters had significantly higher fur, brain and liver Hg levels than males. Similarly, there was not a significant relationship between Hg concentration and age of mink, whereas in otters, Hg concentrations in all three tissues decreased significantly with age. In both species, only a very small percentage of the variability in Hg concentration was explained by age. After adjusting the data for site-to-site differences in Hg levels, Hg concentrations in the fur of mink infected by the parasite, Dioctophyma renale, were found to be significantly higher than Hg levels in uninfected mink.

Preliminary investigation of a possible lung worm (Parafilaroides decorus), fish (Girella nigricans), and marine mammal (Callorhinus ursinus) cycle for San Miguel sea lion virus type 5.

PubMed

Smith, A W; Skilling, D E; Brown, R J

1980-11-01

Colostrum-deprived neonatal Northern fur seal pups (Callorhinus ursinus) were exposed to San Miguel sea lion virus type 5 (SMSV-5) by feeding them fish (Girella nigricans) infected with virus or fish infected with both the sea lion lung worm larvae (Parafilaroides decorus) and virus. Virus infection was demonstrated in 8 of 9 pups, and 1 of these developed a vesicular lesion on the flipper. In this sequence, P decorus larvae exposed to SMSV-5 were fed to G nigricans held at 15 C in a salt water aquarium; 32 days later, these fish were killed, then fed to the fur seal pups. The vesicle developed 22 days subsequent to this and SMSV-5 was reisolated from the lesion. The SMSV-5 was shown to persist for at least 23 days in infected neonatal fur seals. Attempts to establish P decorus infection in Northern fur seal pups were apparently unsuccessful.

Digeneans of northern fur seals Callorhinus ursinus (Pinnipedia: Otariidae) from five subpopulations on St. Paul Island, Alaska.

PubMed

Kuzmina, T A; Tkach, V V; Spraker, T R; Lyons, E T; Kudlai, O

2018-04-01

A parasitological survey of 651 northern fur seals Callorhinus ursinus L. from five subpopulations was conducted on St. Paul Island, Alaska, during July-August 2012-2014. Digenean trematodes were found in 210 of 651 fur seals with a total prevalence of 32.3%. Intensity of infection varied from 1 to 1540 parasites with mean intensity 18.4 ± 111.1 SD and median intensity of 2 specimens per host. Significant differences in prevalence and intensity of infection in northern fur seals between separate rookeries was not observed (Mann-Whitney test; p > 0.05). Four species of digeneans belonging to the families Heterophyidae (Apophallus zalophi Price, 1932, Phocitrema fusiforme Goto and Ozaki, 1930, and Galactosomum ubelakeri (Dailey, 1969)) and Troglotrematidae (Nanophyetus salmincola (Chapin, 1926)) were found. Nanophyetus salmincola is reported from C. ursinus for the first time. We obtained partial 28S rDNA sequences for all digenean species and conducted molecular phylogenetic analysis to demonstrate their phylogenetic relationships.

Building America Case Study: Duct in Conditioned Space in a Dropped Ceiling or Fur-down, Gainesville, Florida (Fact Sheet)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

2014-09-01

Forced air distribution systems (duct systems) typically are installed out of sight for aesthetic reasons, most often in unconditioned areas such as an attic or crawlspace. Any leakage of air to or from the duct system (duct leakage) in unconditioned space not only loses energy, but impacts home and equipment durability and indoor air quality. An obvious solution to this problem is to bring the duct system into the interior of the house, either by sealing the area where the ducts are installed (sealed attic or crawlspace) or by building an interior cavity or chase above the ceiling plane (raisedmore » ceiling or fur-up chase) or below the ceiling plane (dropped ceiling or fur-down) for the duct system. This case study examines one Building America builder partner's implementation of an inexpensive, quick and effective method of building a fur-down or dropped ceiling chase.« less

Pairing ultrasonography with endocrinology to elucidate underlying mechanisms of successful pregnancy in the northern fur seal (Callorhinus ursinus).

PubMed

Shero, Michelle R; Bergfelt, Don R; Testa, J Ward; Adams, Gregg P

2018-01-01

Reproductive success is one of the central tenets of conservation management programs, yet the inability to study underlying physiological processes in a minimally-invasive manner and the unpredictable nature of wild animal populations leaves large gaps in our knowledge of factors critical to successful reproduction in wild species. This study integrated ultrasonography of the reproductive tract and analysis of reproductive hormones in 172 northern fur seals (Callorhinus ursinus) to identify intrinsic factors associated with reinitiating embryonic growth at the end of diapause. Within the first 3-4 weeks of active gestation, pregnant fur seals (n = 126) had a larger corpus luteum and fewer antral follicles than non-pregnant fur seals, or those still in diapause (n = 46). This suggests that the conceptus drives changes in ovarian status to convey its presence to the female. Morphological changes in the reproductive tract associated with pregnancy were not reflected in differences in endocrine profiles (estradiol, estrone, progesterone, and relaxin) between pregnant and non-pregnant individuals. Hormone concentrations correlated more strongly with calendar date than with the presence or size of the conceptus, demonstrating that none of these reproductive hormones were reliable markers for early pregnancy diagnosis. Instead, the northern fur seal's long diestrus may serve to reduce the probability of a temporal mismatch between corpus luteum regression and embryo implantation. Indeed, conception rates were high and confirmed rates of pregnancy loss were relatively low (11%). In this study, minimally-invasive ultrasonography was used in wild pinnipeds to detect very early pregnancy (embryonic vesicles >2 mm) in combination with ovarian and endocrine dynamics at the time of embryo implantation, shedding light on mechanisms for maternal recognition of pregnancy. This study is also the first to track whether these same animals carried the embryo to term, by observing fur seals during the birthing season the following year. Data do not support the notion that decreased pregnancy rates or higher pregnancy loss rates are major contributing factors to the northern fur seal's population decline. Copyright © 2017 Elsevier Inc. All rights reserved.

Retrospective Analysis of Ultrasound-guided Flexible Ureteroscopy in the Management of Calyceal Diverticular Calculi.

PubMed

Zhang, Ji-Qing; Wang, Yong; Zhang, Jun-Hui; Zhang, Xiao-Dong; Xing, Nian-Zeng

2016-09-05

Percutaneous nephrolithotomy (PCNL) is the most widely recommended treatment for calyceal diverticular calculi, providing excellent stone-free results. However, its invasiveness is not negligible considering its major complication rates. Flexible ureteroscopy (FURS) is currently used to treat calyceal diverticula. However, the greatest drawback of FURS is locating the diverticulum since its neck is narrow and concealed. In such a case, the FURS procedure must be converted to PCNL. The aim of this study was to evaluate ultrasound-guided flexible ureteroscopy (UFURS) identifying diverticulum and the management of calyceal diverticular calculi. A retrospective analysis was conducted on 24 patients who had calyceal diverticular calculi. In all 12 patients in the UFURS group, direct FURS failed to find evidence of calyceal diverticula but were confirmed with imaging. The other 12 patients in the PCNL group received PCNL plus fulguration of the diverticular walls. Puncture of calyceal diverticulum was successful in all 12 UFURS patients. Two patients in this group had postoperative residual calculi and two patients developed fever. In the PCNL group, percutaneous renal access and lithotomy were successful in all 12 patients. One patient in this group had residual calculi, one had perirenal hematoma, and two patients developed fever. No significant difference was found in the operating time (UFURS vs. PCNL, 91.8 ± 24.2 vs. 86.3 ± 18.7 min), stone-free rate (UFURS vs. PCNL, 9/12 vs. 10/12), and rate of successful lithotripsy (UFURS vs. PCNL, 10/12 vs. 11/12) between the two groups (all P> 0.05). Postoperative pain scores in the FURS group were significantly lower than that in the PCNL group (2.7 ± 1.2 vs. 6.2 ± 1.5, P< 0.05). Hospital stay in the UFURS group was significantly shorter than that in the PCNL group (3.4 ± 0.8 vs. 5.4 ± 1.0 days, P< 0.05). All patients were symptom-free following surgery (UFURS vs. PCNL, 10/10 vs. 12/12). Ultrasound-guided puncture facilitates identification of calyceal diverticula during FURS and improves the success rate of FURS surgery.

Thermoregulation and the determinants of heat transfer in Colias butterflies.

PubMed

Kingsolver, Joel G; Moffat, Robert J

1982-04-01

As a means of exploring behavioral and morphological adaptations for thermoregulation in Colias butterflies, convective heat transfer coefficients of real and model butterflies were measured in a wind tunnel as a function of wind speed and body orientation (yaw angle). Results are reported in terms of a dimensionless heat transfer coefficient (Nusselt number, Nu) and a dimensionless wind speed (Reynolds number, Re), for a wind speed range typical of that experienced by basking Colias in the field. The resultant Nusselt-Reynolds (Nu-Re) plots thus indicate the rates of heat transfer by forced convection as a function of wind speed for particular model geometries.For Reynolds numbers throughout the measured range, Nusselt numbers for C. eurytheme butterflies are consistently lower than those for long cylinders, and are independent of yaw angle. There is significant variation among individual butterflies in heat transfer coefficients throughout the Re range. Model butterflies without artificial fur have Nu-Re relations similar to those for cylinders. Heat transfer in these models depends upon yaw angle, with higher heat transfer at intermediate yaw angles (30-60°); these yaw effects increase with increasing Reynolds number. Models with artificial fur, like real Colias, have Nusselt numbers which are consistently lower than those for models without fur at given Reynolds numbers throughout the Re range. Unlike real Colias, however, the models with fur do show yaw angle effects similar to those for models without fur.The independence of heat loss from yaw angle for real Colias is consistent with field observations indicating no behavioral orientation to wind direction. The presence of fur on the models reduces heat loss but does not affect yaw dependence. The large individual variation in heat transfer coefficients among butterflies is probably due to differences in fur characteristics rather than to differences in wing morphology.Finally, a physical model of a butterfly was constructed which accurately simulates the body temperatures of basking Colias in the field for a variety of radiation and wind velocity conditions. The success of the butterfly simulator in mimicking Colias thermal characteristics confirms our preliminary understanding of the physical bases for and heat transfer mechanisms underlying thermoregulatory adaptations in these butterflies.

Holocene changes in the trophic ecology of an apex marine predator in the South Atlantic Ocean.

PubMed

Vales, Damián G; Cardona, Luis; Zangrando, Atilio F; Borella, Florencia; Saporiti, Fabiana; Goodall, R Natalie P; de Oliveira, Larissa Rosa; Crespo, Enrique A

2017-02-01

Predators may modify their diets as a result of both anthropogenic and natural environmental changes. Stable isotope ratios of nitrogen and carbon in bone collagen have been used to reconstruct the foraging ecology of South American fur seals (Arctocephalus australis) in the southwestern South Atlantic Ocean since the Middle Holocene, a region inhabited by hunter-gatherers by millennia and modified by two centuries of whaling, sealing and fishing. Results suggest that the isotopic niche of fur seals from Patagonia has not changed over the last two millennia (average for the period: δ 13 C 2200-0BP  = -13.4 ± 0.5‰, δ 15 N 2200-0BP  = 20.6 ± 1.1‰). Conversely, Middle Holocene fur seals fed more pelagically than their modern conspecifics in the Río de la Plata region (δ 13 C 7000BP  = -15.9 ± 0.6‰ vs. δ 13 C PRESENT  = -13.5 ± 0.8‰) and Tierra del Fuego (δ 13 C 6400-4300BP  = -15.4 ± 0.5‰ vs. δ 13 C PRESENT  = -13.2 ± 0.7‰). In the latter region, Middle Holocene fur seals also fed at a higher trophic level than their modern counterparts (δ 15 N 6400-4300BP  = 20.5 ± 0.5‰ vs. δ 15 N PRESENT  = 19.0 ± 1.6‰). Nevertheless, a major dietary shift was observed in fur seals from Tierra del Fuego during the nineteenth century (δ 13 C 100BP  = -17.2 ± 0.3‰, δ 15 N 100BP  = 18.6 ± 0.7‰), when marine primary productivity plummeted and the fur seal population was decimated by sealing. Disentangling the relative roles of natural and anthropogenic factors in explaining this dietary shift is difficult, but certainly the trophic position of fur seals has changed through the Holocene in some South Atlantic regions.

The inability of Bacillus licheniformis perR mutant to grow is mainly due to the lack of PerR-mediated fur repression.

PubMed

Kim, Jung-Hoon; Yang, Yoon-Mo; Ji, Chang-Jun; Ryu, Su-Hyun; Won, Young-Bin; Ju, Shin-Yeong; Kwon, Yumi; Lee, Yeh-Eun; Youn, Hwan; Lee, Jin-Won

2017-06-01

PerR, a member of Fur family protein, is a metal-dependent H 2 O 2 sensing transcription factor that regulates genes involved in peroxide stress response. Industrially important bacterium Bacillus licheniformis contains three PerR-like proteins (PerR BL , PerR2, and PerR3) compared to its close relative Bacillus subtilis. Interestingly, unlike other bacteria including B. subtilis, no authentic perR BL null mutant could be established for B. licheniformis. Thus, we constructed a conditional perR BL mutant using a xylose-inducible promoter, and investigated the genes under the control of PerR BL . PerR BL regulon genes include katA, mrgA, ahpC, pfeT, hemA, fur, and perR as observed for PerR BS . However, there is some variation in the expression levels of fur and hemA genes between B. subtilis and B. licheniformis in the derepressed state. Furthermore, katA, mrgA, and ahpC are strongly induced, whereas the others are only weakly or not induced by H 2 O 2 treatment. In contrast to the B. subtilis perR null mutant which frequently gives rise to large colony phenotype mainly due to the loss of katA, the suppressors of B. licheniformis perR mutant, which can form colonies on LB agar, were all catalase-positive. Instead, many of the suppressors showed increased levels of siderophore production, suggesting that the suppressor mutation is linked to the fur gene. Consistent with this, perR fur double mutant could grow on LB agar without Fe supplementation, whereas perR katA double mutant could only grow on LB agar with Fe supplementation. Taken together, our data suggest that in B. licheniformis, despite the similarity in PerR BL and PerR BS regulon genes, perR is an essential gene required for growth and that the inability of perR null mutant to grow is mainly due to elevated expression of Fur.

THE SHIFTING BASELINE OF NORTHERN FUR SEAL ECOLOGY IN THE NORTHEAST PACIFIC OCEAN

EPA Science Inventory

Historical data provide a baseline against which to judge the significance of recent ecological shifts and guide conservation strategies, especially for species decimated by pre-20th century harvesting. Northern fur seals (NFS; Callorhinus ursinus) are a common pinniped species i...

[Listrophorus gibbus, a fur mite in domestic rabbits (author's transl)].

PubMed

de Vos, J P; Dorrestein, G M

1978-07-01

A case of infection with the fur mite of domestic rabbits, Listrophorus gibbus, is reported. Possible methods of treatment of individual rabbits as well as of colonies of rabbits are reviewed. The presence of Listrophorus gibbus in conjunction with Cheyletiella parasitivorax is also discussed.

The katG mRNA of Mycobacterium tuberculosis and Mycobacterium smegmatis is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation

PubMed Central

Sala, Claudia; Forti, Francesca; Magnoni, Francesca; Ghisotti, Daniela

2008-01-01

Background In Mycobacterium tuberculosis and in Mycobacterium smegmatis the furA-katG loci, encoding the FurA regulatory protein and the KatG catalase-peroxidase, are highly conserved. In M. tuberculosis furA-katG constitute a single operon, whereas in M. smegmatis a single mRNA covering both genes could not be found. In both species, specific 5' ends have been identified: the first one, located upstream of the furA gene, corresponds to transcription initiation from the furA promoter; the second one is the katG mRNA 5' end, located in the terminal part of furA. Results In this work we demonstrate by in vitro transcription and by RNA polymerase Chromatin immunoprecipitation that no promoter is present in the M. smegmatis region covering the latter 5' end, suggesting that it is produced by specific processing of longer transcripts. Several DNA fragments of M. tuberculosis and M. smegmatis were inserted in a plasmid between the sigA promoter and the lacZ reporter gene, and expression of the reporter gene was measured. A polypurine sequence, located four bp upstream of the katG translation start codon, increased beta-galactosidase activity and stabilized the lacZ transcript. Mutagenesis of this sequence led to destabilization of the mRNA. Analysis of constructs, in which the polypurine sequence of M. smegmatis was followed by an increasing number of katG codons, demonstrated that mRNA stability requires translation of at least 20 amino acids. In order to define the requirements for the 5' processing of the katG transcript, we created several mutations in this region and analyzed the 5' ends of the transcripts: the distance from the polypurine sequence does not seem to influence the processing, neither the sequence around the cutting point. Only mutations which create a double stranded region around the processing site prevented RNA processing. Conclusion This is the first reported case in mycobacteria, in which both a polypurine sequence and translation initiation are shown to contribute to mRNA stability. The furA-katG mRNA is transcribed from the furA promoter and immediately processed; this processing is prevented by a double stranded RNA at the cutting site, suggesting that the endoribonuclease responsible for the cleavage cuts single stranded RNA. PMID:18394163

Short-term episodes of imposed fasting have a greater effect on young northern fur seals (Callorhinus ursinus) in summer than in winter

PubMed Central

Rosen, David A. S.; Volpov, Beth L.; Trites, Andrew W.

2014-01-01

An unexpected shortage of food may affect wildlife in a different way depending on the time of year when it occurs. We imposed 48 h fasts on six female northern fur seals (Callorhinus ursinus; ages 6–24 months) to identify times of year when they might be particularly sensitive to interruptions in food supply. We monitored changes in their resting metabolic rates and their metabolic response to thermal challenges, and also examined potential bioenergetic causes for seasonal differences in body mass loss. The pre-fast metabolism of the fur seals while in ambient air or submerged in water at 4°C was higher during summer (June to Sepember) than winter (November to March), and submergence did not significantly increase metabolism, indicating a lack of additional thermoregulatory costs. There was no evidence of metabolic depression following the fasting periods, nor did metabolism increase during the post-fast thermal challenge, suggesting that mass loss did not negatively impact thermoregulatory capacity. However, the fur seals lost mass at greater rates while fasting during the summer months, when metabolism is normally high to facilitate faster growth rates (which would ordinarily have been supported by higher food intake levels). Our findings suggest that summer is a more critical time of year than winter for young northern fur seals to obtain adequate nutrition. PMID:27293642

75 FR 59077 - Airworthiness Directives; GROB-WERKE (Type Certificate Previously Held by BURKHART GROB Luft- und...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-27

... product. The MCAI describes the unsafe condition as: The manufacturer has received a report of a failed... manufacturer has received a report of a failed canopy jettison test, during a regular maintenance check. The... take about 3 work-hours and require parts costing $68, for a cost of $323 per product. We have no way...

EFFECTS OF HEAT AND BROMOCHLOROACETIC ACID ON MALE REPRODUCTION IN HEAT SHOCK FACTOR-1 GENE KNOCKOUT MICE

EPA Science Inventory

Effects of heat and bromochloroacetic acid on male reproduction in heat shock factor-1 gene knockout mice.
Luft JC1, IJ Benjamin2, JB Garges1 and DJ Dix1. 1Reproductive Toxicology Division, USEPA, RTP, NC, 27711 and 2Dept of Internal Medicine, Univ.of Texas Southwestern Med C...

77 FR 64437 - Airworthiness Directives; Burkhart GROB Luft-und Raumfahrt GmbH Sailplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-22

... can remove with metal wool and that has no visible pitting in the base metal. If you cannot remove the corrosion with metal wool or if there is visible pitting in the base metal, we define it as heavy corrosion. (3) If any cracks or heavy corrosion are found during any of the inspections required in paragraph (f...

78 FR 1726 - Airworthiness Directives; Burkhart GROB Luft- und Raumfahrt GmbH Sailplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-09

... purposes of this AD, we define slight corrosion as corrosion you can remove with metal wool and that has no visible pitting in the base metal. If you cannot remove the corrosion with metal wool or if there is visible pitting in the base metal, we define it as heavy corrosion. (3) If any cracks or heavy corrosion...

76 FR 9513 - Airworthiness Directives; BURKHART GROB LUFT-UND Model G 103 C Twin III SL Gliders

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-18

... plate) and tighten it, applying the torque following page 6.12 of the G 103 C TWIN III SL Maintenance...: The torque values and tolerances of the upper pulley wheel grooved nut have been standardized in the..., before further flight, readjust the torque of the upper pulley wheel grooved nut using the updated...

Zeitschrift fur erziehungs--und sozialwissenschaftliche Forschung (Journal for Education and Social Sciences Research), 1984-1988 (11 issues).

ERIC Educational Resources Information Center

Zeitschrift fur erziehungs--und socialwissenschaftliche Forschung (Journal for Education and Social Sciences Research), 1984

1984-01-01

Recognizing a growing globalization of nations and cultures, "Zeitschrift fur erziehungs--und sozialwissenchaftliche Forschung" brings together educational and social science research topics that address the interactions between education and society in their pedagogical, social, physical, economic, legal, and administrative dimensions.…

77 FR 57043 - Regulations Under the Fur Products Labeling Act

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-17

... on purportedly superior European fur-farming practices, which can change and which the Commission... procyonoides is like the common practice of using ``African Lion'' to refer to lions raised in America.\\54\\ \\51... kept for farming purposes. The Convention aims to protect animals against any unnecessary suffering or...

50 CFR 216.74 - Cooperation with Federal officials.

Code of Federal Regulations, 2011 CFR

2011-10-01

... research on the Pribilof Islands who may need assistance in recording tag or other data and collecting tissue or other fur seal samples for research purposes. In addition, Pribilovians who take fur seals for... Pribilof Islands who are responsible for compiling the following information on a daily basis: (a) The...

50 CFR 216.74 - Cooperation with Federal officials.

Code of Federal Regulations, 2010 CFR

2010-10-01

... research on the Pribilof Islands who may need assistance in recording tag or other data and collecting tissue or other fur seal samples for research purposes. In addition, Pribilovians who take fur seals for... Pribilof Islands who are responsible for compiling the following information on a daily basis: (a) The...

Time-of-flight Extreme Environment Diffractometer at the Helmholtz-Zentrum Berlin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Prokhnenko, Oleksandr, E-mail: prokhnenko@helmholtz-berlin.de; Stein, Wolf-Dieter; Bleif, Hans-Jürgen

2015-03-15

The Extreme Environment Diffractometer (EXED) is a new neutron time-of-flight instrument at the BER II research reactor at the Helmholtz-Zentrum Berlin, Germany. Although EXED is a special-purpose instrument, its early construction made it available for users as a general-purpose diffractometer. In this respect, EXED became one of the rare examples, where the performance of a time-of-flight diffractometer at a continuous source can be characterized. In this paper, we report on the design and performance of EXED with an emphasis on the unique instrument capabilities. The latter comprise variable wavelength resolution and wavelength band, control of the incoming beam divergence, themore » possibility to change the angular positions of detectors and their distance to the sample, and use of event recording and offline histogramming. These features combined make EXED easily tunable to the requirements of a particular problem, from conventional diffraction to small angle neutron scattering. The instrument performance is demonstrated by several reference measurements and user experiments.« less

75 FR 21233 - Incidental Takes of Marine Mammals During Specified Activities; Replacement and Repair of Fur...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-23

... intermittent and early season presence through early June. The purpose of the replacement and repair operations... take of northern fur seals hauling out on St. Paul Island during their intermittent and early season... includes both territorial males and non-territorial males. In addition, NMFS estimates intermittent arrival...

76 FR 13550 - Fur Products Labeling Act

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-14

... products of ``relatively small quantity or values from labeling requirements. 15 U.S.C. 69(d). Exercising... things, the economic impact of, and the continuing need for, the Fur Rule provisions; the benefits of the...) Is there a continuing need for the Rules as currently promulgated? Why or why not? (2) What benefits...

How Could a Beaver Start a War?

ERIC Educational Resources Information Center

Millward, Robert

2010-01-01

Students gain a better understanding of war and economics when the variables come alive through stories, artifacts, and paintings. In this article, the author describes a short story about the fur trade which can generate lots of student questions about the fur economics, the Eastern Woodland Indians, trade artifacts, and war. The author also…

50 CFR 216.72 - Restrictions on taking.

Code of Federal Regulations, 2012 CFR

2012-10-01

... disturbance to the rookery or the increased accidental take of female seals. (3) Any taking of adult fur seals or pups, or the intentional taking of subadult female fur seals is prohibited. (4) Only subadult male... debris may only be taken if so directed by NMFS scientists. (d) The scheduling of the harvest is at the...

50 CFR 216.72 - Restrictions on taking.

Code of Federal Regulations, 2013 CFR

2013-10-01

... disturbance to the rookery or the increased accidental take of female seals. (3) Any taking of adult fur seals or pups, or the intentional taking of subadult female fur seals is prohibited. (4) Only subadult male... debris may only be taken if so directed by NMFS scientists. (d) The scheduling of the harvest is at the...

50 CFR 216.72 - Restrictions on taking.

Code of Federal Regulations, 2011 CFR

2011-10-01

... disturbance to the rookery or the increased accidental take of female seals. (3) Any taking of adult fur seals or pups, or the intentional taking of subadult female fur seals is prohibited. (4) Only subadult male... debris may only be taken if so directed by NMFS scientists. (d) The scheduling of the harvest is at the...

50 CFR 216.72 - Restrictions on taking.

Code of Federal Regulations, 2014 CFR

2014-10-01

... disturbance to the rookery or the increased accidental take of female seals. (3) Any taking of adult fur seals or pups, or the intentional taking of subadult female fur seals is prohibited. (4) Only subadult male... debris may only be taken if so directed by NMFS scientists. (d) The scheduling of the harvest is at the...

16 CFR 301.43 - Use of deceptive trade or corporate names, trademarks or graphic representations prohibited.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., trademarks or graphic representations prohibited. 301.43 Section 301.43 Commercial Practices FEDERAL TRADE... Regulations § 301.43 Use of deceptive trade or corporate names, trademarks or graphic representations prohibited. No person shall use in labeling, invoicing or advertising any fur or fur product a trade name...

Cultural and Biological Adaptations to Deprivation: The Northern Ojibwa Case.

ERIC Educational Resources Information Center

Bishop, Charles A.

After the fur trade reached the Ojibwa during the early 17th Century, tribe structure and function rapidly changed. The intensity of social life increased as the Ojibwa and neighboring tribes gathered to exchange fur pelts for European items. Trade became so important that intertribal hostilities arose and an almost unrestrictive slaughter of…

TISSUE DISTRIBUTION OF PCBS AND ORGANOCHLORINE PESTICIDES IN ALASKAN NORTHERN FUR SEALS: COMPARISON OF VARIOUS CONGENER CLASSIFICATION SCHEMES

USDA-ARS?s Scientific Manuscript database

Polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) are believed to adversely affect reproduction and cause health problems in Pinnipeds 1-4. In this study, 145 PCB congeners and OCPs were analyzed in 10 juvenile male northern fur seals, Callorhinus ursinus, collected from Alaskan...

19 CFR 12.60 - Importation prohibited.

Code of Federal Regulations, 2014 CFR

2014-04-01

... TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.60 Importation prohibited. The transportation, importation, sale, or possession of the skins of fur seals or sea otters is prohibited if such skins were taken contrary to the provisions of section 2 of the act of February 26, 1944 (58 Stat. 100...

19 CFR 12.60 - Importation prohibited.

Code of Federal Regulations, 2012 CFR

2012-04-01

... TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.60 Importation prohibited. The transportation, importation, sale, or possession of the skins of fur seals or sea otters is prohibited if such skins were taken contrary to the provisions of section 2 of the act of February 26, 1944 (58 Stat. 100...

19 CFR 12.60 - Importation prohibited.

Code of Federal Regulations, 2011 CFR

2011-04-01

... TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.60 Importation prohibited. The transportation, importation, sale, or possession of the skins of fur seals or sea otters is prohibited if such skins were taken contrary to the provisions of section 2 of the act of February 26, 1944 (58 Stat. 100...

19 CFR 12.60 - Importation prohibited.

Code of Federal Regulations, 2013 CFR

2013-04-01

... TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.60 Importation prohibited. The transportation, importation, sale, or possession of the skins of fur seals or sea otters is prohibited if such skins were taken contrary to the provisions of section 2 of the act of February 26, 1944 (58 Stat. 100...

19 CFR 12.60 - Importation prohibited.

Code of Federal Regulations, 2010 CFR

2010-04-01

... TREASURY SPECIAL CLASSES OF MERCHANDISE Fur-Seal Or Sea-Otter Skins § 12.60 Importation prohibited. The transportation, importation, sale, or possession of the skins of fur seals or sea otters is prohibited if such skins were taken contrary to the provisions of section 2 of the act of February 26, 1944 (58 Stat. 100...

76 FR 72132 - Regulations Under The Fur Products Labeling Act

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-22

... replace it with ``Raccoon Dog.'' HSUS first asserted that the ``true English name'' of an animal should be... name of nyctereutes procyonoidos as ``Raccoon Dog,'' and presented evidence that the scientific... as dog and cat. See Fur Information Council of America Comment at 7-8. Second, the Humane Society of...

Mitogenomics data reveal effective population size, historical bottlenecks, and the effects of hunting on New Zealand fur seals (Arctocephalus forsteri).

PubMed

Emami-Khoyi, Arsalan; Paterson, Adrian M; Hartley, David A; Boren, Laura J; Cruickshank, Robert H; Ross, James G; Murphy, Elaine C; Else, Terry-Ann

2018-05-01

The New Zealand fur seal (Arctocephalus forsteri) passed through a population bottleneck due to commercial sealing during the eighteenth to nineteenth centuries. To facilitate future management options, we reconstructed the demographic history of New Zealand fur seals in a Bayesian framework using maternally inherited, mitochondrial DNA sequences. Mitogenomic data suggested two separate clades (most recent common ancestor 5000 years ago) of New Zealand fur seals that survived large-scale human harvest. Mitochondrial haplotype diversity was high, with 45 singletons identified from 46 individuals although mean nucleotide diversity was low (0.012 ± 0.0061). Variation was not constrained geographically. Analyses of mitogenomes support the hypothesis for a population bottleneck approximately 35 generations ago, which coincides with the peak of commercial sealing. Mitogenomic data are consistent with a pre-human effective population size of approximately 30,000 that first declined to around 10,000 (due to the impact of Polynesian colonization, particularly in the first 100 years of their arrival into New Zealand), and then to 100-200 breeding individuals during peak of commercial sealing.

Rationale for constructing waste-disposal plants at existing enterprises

NASA Astrophysics Data System (ADS)

Strelkov, Alexander; Teplykh, Svetlana; Gorshkalev, Pavel; Proshina, Elizaveta

2017-10-01

The Federal State Statistics Service of the Republic of Tatarstan collected data on registered organizations involved in the fabrication and dyeing of fur. This paper describes wastewater characteristic of an existing enterprise, LLC “Melita”. This enterprise is a factory of a complete technology cycle, with the process starting from fur manufacture and design to implementation. Maximum capacity of the factory is 1800 skins per day, (excluding fur), and its average productivity is 1000 skins per day. Thorough examination of possible methods and schemes for wastewater from fur production purification showed that it is most reasonable to use technology schemes which included the structures of mechanical, physico-chemical and biological purification. As a result, the study provided a new technological scheme for industrial wastewater purification. This scheme offers using chloride barium and sodium hydrocarbons complex as reagents. For LLC “Melita”, the wastewater absorbing pond is the Volga River. Water quality indicators are taken according to the data of the FGBU “Hydrometeorology and Environmental Monitoring Office”. The research also calculates allowable discharge rates and environmental charges in the city sewer networks and ponds.

Radiation pasteurization of mink feed: Effect of irradiated feed on reproductive performance, growth and fur quality of mink

NASA Astrophysics Data System (ADS)

Passey, C. A.; Roy, D.; Savoie, L.; Malo, R.; Wilson, J.

No significant differences were observed in the net birth rate of kits/female between the 7 breeding groups. However, there was reduced incidence (P = 0.05) of kit deaths among the females receiving irradiated feed, and larger kit size (P < 0.0001) at birth particularly for the litter size of 5-8 kits. The second generation minks born to parents receiving feed irradiated to a planned dose of 1 kGy weighed on average about 2.5 % more, and their fur was on average about 1 ± 0.26 cm longer (12 % more males making the top length grade). Moreover, there was no effect of irradiated feed on fur quality. Irradiation of mink feed with subsequent frozen storage of the meat component improved the microbiological quality by decreasing the incidence of Pseudomonas sp. and Salmonella sp. Radiation pasteurization of mink feed (frozen meat to 1 kGy, and dry feed to 2 kGy or more) should therefore help improve feed utilization, keep the animals healthier, and reproducing better without affecting fur quality.

Treatment for residual stones using flexible ureteroscopy and holmium laser lithotripsy after the management of complex calculi with single-tract percutaneous nephrolithotomy.

PubMed

Chen, L; Sha, M-L; Li, D; Zhuo, J; Jiang, C-Y; Zhu, Y-P; Xia, S-J; Lu, J; Shao, Y

2017-04-01

This study validated the effectiveness and safety of the treatment for residual stones using flexible ureteroscopy (fURS) and holmium laser (0.6-1.2 J, 20-30 Hz) lithotripsy via a fiber with a 200-μm core diameter and 0.22 numerical aperture (NA) after the management of complex calculi with single-tract percutaneous nephrolithotomy (PCNL). Between January 2014 and June 2016, 27 consecutive patients with complex calculi underwent fURS and holmium laser lithotripsy after a planned single-tract PCNL. Among the 27 patients with complex calculi, 9 had full staghorn calculi, 7 had partial staghorn calculi, and 11 had multiple calculi. After the first single-tract PCNL session, the mean stone size and mean stone surface area were 18.0 ± 10.7 mm and 181.9 ± 172.2 mm 2 , respectively. Treatment for residual stones with fURS and holmium laser lithotripsy was successfully completed and was performed without intraoperative complications. The mean operative time of the fURS procedure was 69.1 ± 23.6 min, and the mean hospital stay was 5.3 ± 2.4 days. The mean decrease in the hemoglobin level was 7.3 ± 6.5 g/l. After the fURS procedure, the overall stone-free rate was 88.9%. The overall postoperative complication rate was 14.8% (Clavien grade I 11.1%; Clavien grade II 3.7%). The current approach tested here combines the advantages of both PCNL and fURS and effectively manages complex calculi with a high stone-free rate (SFR) (88.9%). This approach also reduced the number of treatment sessions, the number of percutaneous access tracts, and the blood loss and potential morbidity associated with multiple tracts.

Long-Term Seasonal and Interannual Patterns of Marine Mammal Strandings in Subtropical Western South Atlantic

PubMed Central

Prado, Jonatas H. F.; Mattos, Paulo H.; Silva, Kleber G.; Secchi, Eduardo R.

2016-01-01

Understanding temporal patterns of marine mammal occurrence is useful for establishing conservation strategies. We used a 38 yr-long dataset spanning 1976 to 2013 to describe temporal patterns and trends in marine mammal strandings along a subtropical stretch of the east coast of South America. This region is influenced by a transitional zone between tropical and temperate waters and is considered an important fishing ground off Brazil. Generalized Additive Models were used to evaluate the temporal stranding patterns of the most frequently stranded species. Forty species were documented in 12,540 stranding events. Franciscana (n = 4,574), South American fur seal, (n = 3,419), South American sea lion (n = 2,049), bottlenose dolphins (n = 293) and subantarctic fur seal (n = 219) were the most frequently stranded marine mammals. The seasonality of strandings of franciscana and bottlenose dolphin coincided with periods of higher fishing effort and strandings of South American and subantarctic fur seals with post-reproductive dispersal. For South American sea lion the seasonality of strandings is associated with both fishing effort and post-reproductive dispersal. Some clear seasonal patterns were associated with occurrence of cold- (e.g. subantarctic fur seal) and warm-water (e.g. rough-toothed dolphin) species in winter and summer, respectively. Inter-annual increases in stranding rate were observed for franciscana and South American fur seal and these are likely related to increased fishing effort and population growth, respectively. For subantarctic fur seal the stranding rate showed a slight decline while for bottlenose dolphin it remained steady. No significant year to year variation in stranding rate was observed for South American sea lion. The slight decrease in frequency of temperate/polar marine mammals and the increased occurrence of subtropical/tropical species since the late 1990s might be associated with environmental changes linked to climate change. This long-term study indicates that temporal stranding patterns of marine mammals might be explained by either fishing-related or environmental factors. PMID:26814667

Long-Term Seasonal and Interannual Patterns of Marine Mammal Strandings in Subtropical Western South Atlantic.

PubMed

Prado, Jonatas H F; Mattos, Paulo H; Silva, Kleber G; Secchi, Eduardo R

2016-01-01

Understanding temporal patterns of marine mammal occurrence is useful for establishing conservation strategies. We used a 38 yr-long dataset spanning 1976 to 2013 to describe temporal patterns and trends in marine mammal strandings along a subtropical stretch of the east coast of South America. This region is influenced by a transitional zone between tropical and temperate waters and is considered an important fishing ground off Brazil. Generalized Additive Models were used to evaluate the temporal stranding patterns of the most frequently stranded species. Forty species were documented in 12,540 stranding events. Franciscana (n = 4,574), South American fur seal, (n = 3,419), South American sea lion (n = 2,049), bottlenose dolphins (n = 293) and subantarctic fur seal (n = 219) were the most frequently stranded marine mammals. The seasonality of strandings of franciscana and bottlenose dolphin coincided with periods of higher fishing effort and strandings of South American and subantarctic fur seals with post-reproductive dispersal. For South American sea lion the seasonality of strandings is associated with both fishing effort and post-reproductive dispersal. Some clear seasonal patterns were associated with occurrence of cold- (e.g. subantarctic fur seal) and warm-water (e.g. rough-toothed dolphin) species in winter and summer, respectively. Inter-annual increases in stranding rate were observed for franciscana and South American fur seal and these are likely related to increased fishing effort and population growth, respectively. For subantarctic fur seal the stranding rate showed a slight decline while for bottlenose dolphin it remained steady. No significant year to year variation in stranding rate was observed for South American sea lion. The slight decrease in frequency of temperate/polar marine mammals and the increased occurrence of subtropical/tropical species since the late 1990s might be associated with environmental changes linked to climate change. This long-term study indicates that temporal stranding patterns of marine mammals might be explained by either fishing-related or environmental factors.

Management of calyceal diverticular calculi: a comparison of percutaneous nephrolithotomy and flexible ureterorenoscopy.

PubMed

Bas, Okan; Ozyuvali, Ekrem; Aydogmus, Yasin; Sener, Nevzat Can; Dede, Onur; Ozgun, Serhat; Hizli, Fatih; Senocak, Cagri; Bozkurt, Omer Faruk; Basar, Halil; Imamoglu, Abdurrahim

2015-04-01

To compare the outcomes in patients who have been treated with flexible ureterorenoscopy (f-URS) and percutaneous nephrolithotomy (PNL) in managing stone-bearing caliceal diverticula. Between April 2007 and October 2013, we performed a retrospective analysis of 54 evaluable patients (28 women and 26 men) with symptomatic stone-bearing caliceal diverticula, who underwent PNL (n = 29) or F-URS (n = 25) in four referral hospitals in Turkey. The groups were compared with respect to demographics, stone location/size, success rate, stone-free status, symptom-free status, complication rates, and hospital stay. The average stone burden preoperatively was significantly larger in patients who were treated with PNL, with the average size for f-URS being 154 ± 77 mm(2) and that for PNL being 211 ± 97 mm(2) (p = 0.023). Symptom-free rates, success rates, stone-free rates and clinically insignificant residual fragments were similar between the groups (p = 0.880 vs. p = 0.537 vs. p = 0.539, and p = 0.877, respectively). There was no statistical difference between the groups for minor complications (p = 0.521) but no major complication (Clavien III-V) occured in the f-URS group; although there were three major complications (10.3 %) (Clavien III) in the PNL group (p < 0.001). Hospitalization time per patient was 1.04 ± 0.20 days in the f-URS group, while it was 3.86 ± 1.94 days in the PNL group (p < 0.001). Even though this study clearly shows that both techniques have high overall success and symptom-free rates with similar complication rates for stone-bearing calyceal diverticulum, major complication rates may suggest consideration of the invasiveness of PNL. The f-URS procedure is advantageous with respect to a shorter hospital stay and absence of major complications. Therefore, it should be emphasized that the location of the stone and diverticula is an important factor for the selection of the procedure.

Flexible Ureterorenoscopy versus Mini-Percutaneous Nephrolithotomy for the Treatment of Renal Stones.

PubMed

Ergin, Giray; Kirac, Mustafa; Kopru, Burak; Ebiloglu, Turgay; Biri, Hasan

2018-04-22

To compare the pain status and stone free rates of flexible ureterorenoscopy (F-URS) versus mini-percutaneousnephrolithotomy (mini-PNL) for the treatment of 1-to 2-cm renal stones. This study was retrospectively designed with match paired method. Between January 2013 and December 2016, 387 patients underwent stone surgery for renal stones, 45 patients underwent FURS and 45 patients underwent mini-PNL. 90 patients were divided into two groups according to the surgical procedures. Group 1 patients underwent F-URS, and Group 2 patients underwent mini-PNL. During the intraoperative andpostoperative periods, pain management for all patients was standardized. Pain scores were determined using a visual analogue scale (VAS) completed at 2, 6, 12 and 24 hours postoperatively. The stone free status, hemoglobin levels, fluoroscopy time (FT), operation time (OT), hospitalization time (HT), return to work time (RWT), and complications were noted for each patient. Of all patients, the mean age was 41.1 ± 12.1 years and the mean stone size was 13.9 ± 2.9 mm. The VAS scores were significantly higher in the mini-PNL group at 2, 6, 12 and 24 hours (P < .05). The stone-free status and complication rates were similar between the two groups (P > .05); however, the hemoglobin decreases and the fluoroscopy, operation, hospitalization and return to work times were higher in the mini-PNL group than in the F-URS group (P < .05). F-URS is less painful than mini-PNL for the treatment of 1- to 2-cm renal stones. However, the stone free rate is similar between the two procedures while mini-PNL is superior in terms of fluoroscopy, operation, hospitalization and return to work duration. We think that F-URS is more comfortable and less painful than mini-PNL and achieves a similar stone free rate for the treatment of 1- to 2-cm renal stones.

Campylobacter jejuni dsb gene expression is regulated by iron in a Fur-dependent manner and by a translational coupling mechanism

PubMed Central

2011-01-01

Background Many bacterial extracytoplasmic proteins are stabilized by intramolecular disulfide bridges that are formed post-translationally between their cysteine residues. This protein modification plays an important role in bacterial pathogenesis, and is facilitated by the Dsb (disulfide bond) family of the redox proteins. These proteins function in two parallel pathways in the periplasmic space: an oxidation pathway and an isomerization pathway. The Dsb oxidative pathway in Campylobacter jejuni is more complex than the one in the laboratory E. coli K-12 strain. Results In the C. jejuni 81-176 genome, the dsb genes of the oxidative pathway are arranged in three transcriptional units: dsbA2-dsbB-astA, dsbA1 and dba-dsbI. Their transcription responds to an environmental stimulus - iron availability - and is regulated in a Fur-dependent manner. Fur involvement in dsb gene regulation was proven by a reporter gene study in a C. jejuni wild type strain and its isogenic fur mutant. An electrophoretic mobility shift assay (EMSA) confirmed that analyzed genes are members of the Fur regulon but each of them is regulated by a disparate mechanism, and both the iron-free and the iron-complexed Fur are able to bind in vitro to the C. jejuni promoter regions. This study led to identification of a new iron- and Fur-regulated promoter that drives dsbA1 gene expression in an indirect way. Moreover, the present work documents that synthesis of DsbI oxidoreductase is controlled by the mechanism of translational coupling. The importance of a secondary dba-dsbI mRNA structure for dsbI mRNA translation was verified by estimating individual dsbI gene expression from its own promoter. Conclusions The present work shows that iron concentration is a significant factor in dsb gene transcription. These results support the concept that iron concentration - also through its influence on dsb gene expression - might control the abundance of extracytoplasmic proteins during different stages of infection. Our work further shows that synthesis of the DsbI membrane oxidoreductase is controlled by a translational coupling mechanism. The dba expression is not only essential for the translation of the downstream dsbI gene, but also Dba protein that is produced might regulate the activity and/or stability of DsbI. PMID:21787430

Campylobacter jejuni dsb gene expression is regulated by iron in a Fur-dependent manner and by a translational coupling mechanism.

PubMed

Grabowska, Anna D; Wandel, Michał P; Łasica, Anna M; Nesteruk, Monika; Roszczenko, Paula; Wyszyńska, Agnieszka; Godlewska, Renata; Jagusztyn-Krynicka, Elzbieta K

2011-07-25

Many bacterial extracytoplasmic proteins are stabilized by intramolecular disulfide bridges that are formed post-translationally between their cysteine residues. This protein modification plays an important role in bacterial pathogenesis, and is facilitated by the Dsb (disulfide bond) family of the redox proteins. These proteins function in two parallel pathways in the periplasmic space: an oxidation pathway and an isomerization pathway. The Dsb oxidative pathway in Campylobacter jejuni is more complex than the one in the laboratory E. coli K-12 strain. In the C. jejuni 81-176 genome, the dsb genes of the oxidative pathway are arranged in three transcriptional units: dsbA2-dsbB-astA, dsbA1 and dba-dsbI. Their transcription responds to an environmental stimulus - iron availability - and is regulated in a Fur-dependent manner. Fur involvement in dsb gene regulation was proven by a reporter gene study in a C. jejuni wild type strain and its isogenic fur mutant. An electrophoretic mobility shift assay (EMSA) confirmed that analyzed genes are members of the Fur regulon but each of them is regulated by a disparate mechanism, and both the iron-free and the iron-complexed Fur are able to bind in vitro to the C. jejuni promoter regions. This study led to identification of a new iron- and Fur-regulated promoter that drives dsbA1 gene expression in an indirect way. Moreover, the present work documents that synthesis of DsbI oxidoreductase is controlled by the mechanism of translational coupling. The importance of a secondary dba-dsbI mRNA structure for dsbI mRNA translation was verified by estimating individual dsbI gene expression from its own promoter. The present work shows that iron concentration is a significant factor in dsb gene transcription. These results support the concept that iron concentration - also through its influence on dsb gene expression - might control the abundance of extracytoplasmic proteins during different stages of infection. Our work further shows that synthesis of the DsbI membrane oxidoreductase is controlled by a translational coupling mechanism. The dba expression is not only essential for the translation of the downstream dsbI gene, but also Dba protein that is produced might regulate the activity and/or stability of DsbI.

Exploring a Model of Situated Professional Development: Impact on Classroom Practice

ERIC Educational Resources Information Center

Singer, Jonathan; Lotter, Christine; Feller, Robert; Gates, Harry

2011-01-01

A hallmark of current science education reform involves teaching through inquiry. However, the widespread use of inquiry-based instruction in many classrooms has not occurred (Roehrig and Luft in "Int J Sci Educ" 26:3-24, 2004; Schneider et al. in "J Res Sci Teach" 42:283-312, 2005). The purpose of this study was to investigate the impact of a…

Measuring short distance dispersal of Alliaria petiolata and determining potential long distance dispersal mechanisms

PubMed Central

Anderson, Roger C.

2018-01-01

Introduction Alliaria petiolata, an herbaceous plant, has invaded woodlands in North America. Its ecology has been thoroughly studied, but an overlooked aspect of its biology is seed dispersal distances and mechanisms. We measured seed dispersal distances in the field and tested if epizoochory is a potential mechanism for long-distance seed dispersal. Methods Dispersal distances were measured by placing seed traps in a sector design around three seed point sources, which consisted of 15 second-year plants transplanted within a 0.25 m radius circle. Traps were placed at intervals ranging from 0.25–3.25 m from the point source. Traps remained in the field until a majority of seeds were dispersed. Eight probability density functions were fitted to seed trap counts via maximum likelihood. Epizoochory was tested as a potential seed dispersal mechanism for A. petiolata through a combination of field and laboratory experiments. To test if small mammals transport A. petiolata seeds in their fur, experimental blocks were placed around dense A. petiolata patches. Each block contained a mammal inclusion treatment (MIT) and control. The MIT consisted of a wood-frame (31 × 61× 31 cm) covered in wire mesh, except for the two 31 × 31 cm ends, placed over a germination tray filled with potting soil. A pan filled with bait was placed in the center of the tray. The control frame (11 × 31 × 61 cm) was placed over a germination tray and completely covered in wire mesh to exclude animal activity. Treatments were in the field for peak seed dispersal. In March, trays were moved to a greenhouse and A. petiolata seedlings were counted and then compared between treatments. To determine if A. petiolata seeds attach to raccoon (Procyon lotor) and white-tailed deer (Odocoileus virginianus) fur, wet and dry seeds were dropped onto wet and dry fur. Furs were rotated 180 degrees and the seeds that remained attached were counted. To measure seed retention, seeds were dropped on furs and rotated as before, then the furs were agitated for one hour. The seeds retained in the fur were counted. Results For the seed dispersal experiment, the 2Dt function provided the best fit and was the most biologically meaningful. It predicted that seed density rapidly declined with distance from the point source. Mean dispersal distance was 0.52 m and 95% of seeds dispersed within 1.14 m. The epizoochory field experiment showed increased mammal activity and A. petiolata seedlings in germination trays of the MIT compared to control. Laboratory studies showed 3–26% of seeds were attached and retained by raccoon and deer fur. Retention significantly increased if either seed or fur were wet (57–98%). Discussion Without animal seed vectors, most seeds fall within a short distance of the seed source; however, long distance dispersal may be accomplished by epizoochory. Our data are consistent with A. petiolata’s widespread distribution and development of dense clusters of the species in invaded areas. PMID:29576955

An der Schwelle zur Zweisprachigkeit: Fremdsprachenunterricht fur Fortgeschrittene (On the Threshold of Bilingualism: Foreign Language Learning for Advanced Students).

ERIC Educational Resources Information Center

Kubler, Silvia, Ed.; Portmann, Paul R., Ed.

1994-01-01

This collection of articles on Bilingualism includes: "Fremdsprachenunterricht fur Fortgeschrittene: ein Uberblick" (Foreign Language Learning for Advanced Students: An Overview) (Paul R. Portmann); "Never Mind the Width, Feel the Quality: From Quantity to Quality in Language Teaching at Advanced Levels" (Mike Makosch); "Irren ist menschlich: Ein…

Field Study on Moisture Problems in Exterior Walls of Family Housing Units at Naval Air Station Pensacola, Florida.

DTIC Science & Technology

1984-02-01

exterior exposed concrete block walls with 2 inch (nominal) furring, 1 inch cellular board ( expanded polystyrene ) insulation, and gypsum board finish, as...furring strips, and new expanded polystyrene board thermal insu- lation and new gypsum board were installed. The purpose of the coating on the concrete

Tissue Distribution of Polychlorinated Biphenyls and Organochlorine Pesticides and Potential Toxicity to Alaskan Northern Fur Seals Assessed Using PCBs Congener Specific Mode of Action Schemes

USDA-ARS?s Scientific Manuscript database

The concentrations of 145 polychlorinated biphenyl (PCB) congeners were measured using gas chromatography-ion trap mass spectrometry in 8 different tissues (blubber, brain, heart, kidney, liver, lung, muscle, and reproductive tissues) of 10 Alaskan northern fur seals. The mean concentrations of bot...

43 CFR 2916.2-4 - Termination of lease; cancellation.

Code of Federal Regulations, 2014 CFR

2014-10-01

... purpose other than the rearing of fur-bearing animals as authorized. No lease will be canceled until the... property may not be removed from the lands, except fur-bearing animals disposed of in the regular course of...) of this section, which he has a right to remove; if not removed or otherwise disposed of within the...

43 CFR 2916.2-4 - Termination of lease; cancellation.

Code of Federal Regulations, 2012 CFR

2012-10-01

... purpose other than the rearing of fur-bearing animals as authorized. No lease will be canceled until the... property may not be removed from the lands, except fur-bearing animals disposed of in the regular course of...) of this section, which he has a right to remove; if not removed or otherwise disposed of within the...

43 CFR 2916.2-4 - Termination of lease; cancellation.

Code of Federal Regulations, 2013 CFR

2013-10-01

... purpose other than the rearing of fur-bearing animals as authorized. No lease will be canceled until the... property may not be removed from the lands, except fur-bearing animals disposed of in the regular course of...) of this section, which he has a right to remove; if not removed or otherwise disposed of within the...

43 CFR 2916.2-4 - Termination of lease; cancellation.

Code of Federal Regulations, 2011 CFR

2011-10-01

... purpose other than the rearing of fur-bearing animals as authorized. No lease will be canceled until the... property may not be removed from the lands, except fur-bearing animals disposed of in the regular course of...) of this section, which he has a right to remove; if not removed or otherwise disposed of within the...

16 CFR 301.10 - Use of term “Broadtail-processed Lamb” permitted.

Code of Federal Regulations, 2010 CFR

2010-01-01

... “Broadtail-processed Lamb” permitted. The term Broadtail-processed Lamb may be used to describe the skin of a lamb which has been sheared, leaving a moire hair pattern on the pelt having the appearance of the true fur pattern of “Broadtail Lamb”; as for example: Dyed Broadtail-processed Lamb Fur origin: Argentina ...

16 CFR 301.48a - Guaranties not received in good faith.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Guaranties not received in good faith. 301... received in good faith. A guaranty shall not be deemed to have been received in good faith within the... required label, required invoice and advertisement relating to the fur product or fur so guaranteed; (b) If...

Institute for Science Education. Institut fur die Padagogik der Naturwissenschaften an der Universitat Kiel. IPN Report-in-Brief 11. 3rd Edition.

ERIC Educational Resources Information Center

Blansdorf, Klaus, Ed.

The Institut fur die Padagogik der Naturwissenschaften (IPN) is the research institute for science education, with a national function in the Federal Republic of Germany. The IPN consists of biology education, chemistry education, physics education, educational science, research methodology/statistics, and administration/general services…

High-resolution mapping of the NO2 spatial distribution over Belgian urban areas based on airborne APEX remote sensing

NASA Astrophysics Data System (ADS)

Tack, Frederik; Merlaud, Alexis; Iordache, Marian-Daniel; Danckaert, Thomas; Yu, Huan; Fayt, Caroline; Meuleman, Koen; Deutsch, Felix; Fierens, Frans; Van Roozendael, Michel

2017-05-01

We present retrieval results of tropospheric nitrogen dioxide (NO2) vertical column densities (VCDs), mapped at high spatial resolution over three Belgian cities, based on the DOAS analysis of Airborne Prism EXperiment (APEX) observations. APEX, developed by a Swiss-Belgian consortium on behalf of ESA (European Space Agency), is a pushbroom hyperspectral imager characterised by a high spatial resolution and high spectral performance. APEX data have been acquired under clear-sky conditions over the two largest and most heavily polluted Belgian cities, i.e. Antwerp and Brussels on 15 April and 30 June 2015. Additionally, a number of background sites have been covered for the reference spectra. The APEX instrument was mounted in a Dornier DO-228 aeroplane, operated by Deutsches Zentrum für Luft- und Raumfahrt (DLR). NO2 VCDs were retrieved from spatially aggregated radiance spectra allowing urban plumes to be resolved at the resolution of 60 × 80 m2. The main sources in the Antwerp area appear to be related to the (petro)chemical industry while traffic-related emissions dominate in Brussels. The NO2 levels observed in Antwerp range between 3 and 35 × 1015 molec cm-2, with a mean VCD of 17.4 ± 3.7 × 1015 molec cm-2. In the Brussels area, smaller levels are found, ranging between 1 and 20 × 1015 molec cm-2 and a mean VCD of 7.7 ± 2.1 × 1015 molec cm-2. The overall errors on the retrieved NO2 VCDs are on average 21 and 28 % for the Antwerp and Brussels data sets. Low VCD retrievals are mainly limited by noise (1σ slant error), while high retrievals are mainly limited by systematic errors. Compared to coincident car mobile-DOAS measurements taken in Antwerp and Brussels, both data sets are in good agreement with correlation coefficients around 0.85 and slopes close to unity. APEX retrievals tend to be, on average, 12 and 6 % higher for Antwerp and Brussels, respectively. Results demonstrate that the NO2 distribution in an urban environment, and its fine-scale variability, can be mapped accurately with high spatial resolution and in a relatively short time frame, and the contributing emission sources can be resolved. High-resolution quantitative information about the atmospheric NO2 horizontal variability is currently rare, but can be very valuable for (air quality) studies at the urban scale.

Cb-LIKE - Thunderstorm forecasts up to six hours with fuzzy logic

NASA Astrophysics Data System (ADS)

Köhler, Martin; Tafferner, Arnold

2016-04-01

Thunderstorms with their accompanying effects like heavy rain, hail, or downdrafts cause delays and flight cancellations and therefore high additional cost for airlines and airport operators. A reliable thunderstorm forecast up to several hours could provide more time for decision makers in air traffic for an appropriate reaction on possible storm cells and initiation of adequate counteractions. To provide the required forecasts Cb-LIKE (Cumulonimbus-LIKElihood) has been developed at the DLR (Deutsches Zentrum für Luft- und Raumfahrt) Institute of Atmospheric Physics. The new algorithm is an automated system which designates areas with possible thunderstorm development by using model data of the COSMO-DE weather model, which is driven by the German Meteorological Service (DWD). A newly developed "Best-Member- Selection" method allows the automatic selection of that particular model run of a time-lagged COSMO- DE model ensemble, which matches best the current thunderstorm situation. Thereby the application of the best available data basis for the calculation of the thunderstorm forecasts by Cb-LIKE is ensured. Altogether there are four different modes for the selection of the best member. Four atmospheric parameters (CAPE, vertical wind velocity, radar reflectivity and cloud top temperature) of the model output are used within the algorithm. A newly developed fuzzy logic system enables the subsequent combination of the model parameters and the calculation of a thunderstorm indicator within a value range of 12 up to 88 for each grid point of the model domain for the following six hours in one hour intervals. The higher the indicator value the more the model parameters imply the development of thunderstorms. The quality of the Cb-LIKE thunderstorm forecasts was evaluated by a substantial verification using a neighborhood verification approach and multi-event contingency tables. The verification was performed for the whole summer period of 2012. On the basis of a deterministic object comparison with heavy precipitation cells observed by the radar-based thunderstorm tracking algorithm Rad-TRAM, several verification scores like BIAS, POD, FAR and CSI were calculated to identify possible advantages of the new algorithm. The presentation illustrates in detail the concept of the Cb-LIKE algorithm with regard to the fuzzy logic system and the Best-Member-Selection. Additionally some case studies and the most important results of the verification will be shown. The implementation of the forecasts into the DLR WxFUSION system, an user oriented forecasting system for air traffic, will also be included.

Assessment of HRSC Digital Terrain Models Produced for the South Polar Residual Cap

NASA Astrophysics Data System (ADS)

Putri, Alfiah Rizky Diana; Sidiropoulos, Panagiotis; Muller, Jan-Peter

2017-04-01

The current Digital Terrain Models available for Mars consist of NASA MOLA (Mars Orbital Laser Altimeter) Digital Terrain Models with an average resolution of 112 m/ pixel (512 pixels/degree) for the polar region. The ESA/DLR High Resolution Stereo Camera is currently orbiting Mars and mapping its surface, 98% with resolution of ≤100 m/pixel and better and 100% at lower resolution [1]. It is possible to produce Digital Terrain Models from HRSC images using various methods. In this study, the method developed on Kim and Muller [2] which uses the VICAR open source program together with photogrammetry sofrware from DLR (Deutschen Zentrums für Luft- und Raumfahrt) with image matching based on the GOTCHA (Gruen-Otto-Chau) algorithm [3]. Digital Terrain Models have been processed over the South Pole with emphasis on areas around South Polar Residual Cap from High Resolution Stereo Camera images [4]. Digital Terrain Models have been produced for 31 orbits out of 149 polar orbits available. This study analyses the quality of the DTMs including an assessment of accuracy of elevations using the MOLA MEGDR (Mission Experiment Gridded Data Records) which has roughly 42 million MOLA PEDR (Precision Experiment Data Records) points between latitudes of 78 o -90 o S. The issues encountered in the production of Digital Terrain Models will be described and the statistical results and assessment method will be presented. The resultant DTMs will be accessible via http://i-Mars.eu/web-GIS References: [1] Neukum, G. et. al, 2004. Mars Express: The Scientific Payload pp. 17-35. [2] Kim, J.-R. and J.-P. Muller. 2009. PSS vol. 57, pp. 2095-2112. [3] Shin, D. and J.-P. Muller. 2012. Pattern Recognition, 45(10), 3795 -3809. [4] Putri, A.R. D., et al., Int. Arch. Photogramm. Remote Sens. Spatial Inf. Sci., XLI-B4, 463-469 Acknowledgements: The research leading to these results has received partial funding from the STFC "MSSL Consolidated Grant" ST/K000977/1 and partial support from the European Union's Seventh Framework Programme (FP7/2007-2013) under iMars grant agreement n ˚ 607379. The first author would like to acknowledge support for her studies from Indonesia Endowment Fund for Education (LPDP), Ministry of Finance, Republic of Indonesia. The authors would also like to thank Alexander Dumke (Freie Universitaet Berlin) for providing the EXTORI exterior orientation elements which were critical in the production of accuracy geolocations.

Characteristics of an airborne demonstrator for MERLIN

NASA Astrophysics Data System (ADS)

Amediek, A.; Büdenbender, C.; Ehret, G.; Fix, A.; Kiemle, C.; Quatrevalet, M.; Wirth, M.; Dieter, H.; Löhring, J.; Klein, V.

2012-12-01

After three years development time, first test measurements on DLR's (Deutsches Zentrum für Luft- und Raumfahrt) CO2 and CH4 airborne Lidar have started. It is an integrated path differential absorption (IPDA) lidar for the simultaneous measurement of CO2 and CH4 columns, designed for operation onboard the new German research aircraft HALO. In the framework of the project "CHARM-F", funded by the German ministry of education and research, the lidar was developed in collaboration with Fraunhofer Institut für Lasertechnik and Kayser-Threde. Due to the special features of the aircraft, such as the maximum flight altitude of 15 km and its long range, as well as the special design of the lidar, the system is particularly suitable to be an airborne demonstrator for the French-German MERLIN project, a spaceborne IPDA lidar sounder for methane. The layout of the receiver optics allows a large field of view, i.e. a large laser footprint on ground is possible, comparable to the size obtained by a spaceborne system. So, important features that come along with ground reflectivity issues, such as albedo variations on different spatial scales, can be taken into account in the same way and can be investigated in detail. Furthermore, two detector types are used, PIN photodiodes and APDs, each with specially adapted telescopes, to compare their respective properties. The basic design of the transmitter is identical to the one envisaged for MERLIN. Also important subsystems of the presented lidar, like wavelengths stabilization and output power monitoring, can serve as demonstrators for the satellite system. The main features of the airborne system are: Two almost identical laser systems for CH4 and CO2. Nd:YAG lasers serve as the pump sources for optical parametric oscillators (OPO), injection seeded by laser diodes, to generate the desired online and offline wavelengths in single mode operation. The online wavelength is tuned to an absorption line of the measured trace gas, the offline to a wavelength nearby showing much less absorption (DIAL principle). Pulsed operation of the laser transmitters allows proper separation of atmospheric influences (e.g. aerosol and clouds) as well as precise ranging, which is crucial for the retrieval of the column averaged gas mixing ratio. Due to double pulse operation with a short temporal separation (250 μs), the areas on ground illuminated by subsequent online and offline laser pulses show only little spatial shifts. Currently, the system is being set into operation, and in the course of this all relevant specifications are being characterized. Extensive tests and validation missions are planned for 2013.

Combination of a fusogenic glycoprotein, prodrug activation, and oncolytic herpes simplex virus for enhanced local tumor control.

PubMed

Simpson, Guy R; Han, Ziqun; Liu, Binlei; Wang, Yibing; Campbell, Gregor; Coffin, Robert S

2006-05-01

We have previously developed an oncolytic herpes simplex virus-1 based on a clinical virus isolate, which was deleted for ICP34.5 to provide tumor selected replication and ICP47 to increase antigen presentation as well as tumor selective virus replication. A phase I/II clinical trial using a version of this virus expressing granulocyte macrophage colony-stimulating factor has shown promising results. The work reported here aimed to develop a version of this virus in which local tumor control was further increased through the combined expression of a highly potent prodrug activating gene [yeast cytosine deaminase/uracil phospho-ribosyltransferase fusion (Fcy::Fur)] and the fusogenic glycoprotein from gibbon ape leukemia virus (GALV), which it was hoped would aid the spread of the activated prodrug through the tumor. Viruses expressing the two genes individually or in combination were constructed and tested, showing (a) GALV and/or Fcy::Fur expression did not affect virus growth; (b) GALV expression causes cell fusion and increases the tumor cell killing at least 30-fold in vitro and tumor shrinkage 5- to 10-fold in vivo; (c) additional expression of Fcy::Fur combined with 5-fluorocytosine administration improves tumor shrinkage further. These results indicate, therefore, that the combined expression of the GALV protein and Fcy::Fur provides a highly potent oncolytic virus with improved capabilities for local tumor control. It is intended to enter the GALV/Fcy::Fur expressing virus into clinical development for the treatment of tumor types, such as pancreatic or lung cancer, where local control would be anticipated to be clinically advantageous.

Measure Guideline: Summary of Interior Ducts in New Construction, Including an Efficient, Affordable Method to Install Fur-Down Interior Ducts

DOE Office of Scientific and Technical Information (OSTI.GOV)

Beal, D.; McIlvaine , J.; Fonorow, K.

2011-11-01

This document illustrates guidelines for the efficient installation of interior duct systems in new housing, including the fur-up chase method, the fur-down chase method, and interior ducts positioned in sealed attics or sealed crawl spaces. This document illustrates guidelines for the efficient installation of interior duct systems in new housing. Interior ducts result from bringing the duct work inside a home's thermal and air barrier. Architects, designers, builders, and new home buyers should thoroughly investigate any opportunity for energy savings that is as easy to implement during construction, such as the opportunity to construct interior duct work. In addition tomore » enhanced energy efficiency, interior ductwork results in other important advantages, such as improved indoor air quality, increased system durability and increased homeowner comfort. While the advantages of well-designed and constructed interior duct systems are recognized, the implementation of this approach has not gained a significant market acceptance. This guideline describes a variety of methods to create interior ducts including the fur-up chase method, the fur-down chase method, and interior ducts positioned in sealed attics or sealed crawl spaces. As communication of the intent of an interior duct system, and collaboration on its construction are paramount to success, this guideline details the critical design, planning, construction, inspection, and verification steps that must be taken. Involved in this process are individuals from the design team; sales/marketing team; and mechanical, insulation, plumbing, electrical, framing, drywall and solar contractors.« less

Role of the Fur Regulon in Iron Transport in Bacillus subtilis

PubMed Central

Ollinger, Juliane; Song, Kyung-Bok; Antelmann, Haike; Hecker, Michael; Helmann, John D.

2006-01-01

The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding ∼40 genes. We investigated the physiological roles of Fur-regulated genes by the construction of null mutations in 14 transcription units known or predicted to function in siderophore biosynthesis or iron uptake. We demonstrate that ywbLMN, encoding an elemental iron uptake system orthologous to the copper oxidase-dependent Fe(III) uptake system of Saccharomyces cerevisiae, is essential for growth in low iron minimal medium lacking citric acid. 2,3-Dihydroxybenzoyl-glycine (Itoic acid), the siderophore precursor produced by laboratory strains of B. subtilis, is of secondary importance. In the presence of citrate, the YfmCDEF ABC transporter is required for optimal growth. B. subtilis is unable to grow in minimal medium containing the iron chelator EDDHA unless the ability to synthesize the intact bacillibactin siderophore is restored (by the introduction of a functional sfp gene) or exogenous siderophores are provided. Utilization of the catecholate siderophores bacillibactin and enterobactin requires the FeuABC importer and the YusV ATPase. Utilization of hydroxamate siderophores requires the FhuBGC ABC transporter together with the FhuD (ferrichrome) or YxeB (ferrioxamine) substrate-binding proteins. Growth with schizokinen or arthrobactin is at least partially dependent on the YfhA YfiYZ importer and the YusV ATPase. We have also investigated the effects of a fur mutation on the proteome and documented the derepression of 11 Fur-regulated proteins, including a newly identified thioredoxin reductase homolog, YcgT. PMID:16672620

Role of the Fur regulon in iron transport in Bacillus subtilis.

PubMed

Ollinger, Juliane; Song, Kyung-Bok; Antelmann, Haike; Hecker, Michael; Helmann, John D

2006-05-01

The Bacillus subtilis ferric uptake regulator (Fur) protein mediates the iron-dependent repression of at least 20 operons encoding approximately 40 genes. We investigated the physiological roles of Fur-regulated genes by the construction of null mutations in 14 transcription units known or predicted to function in siderophore biosynthesis or iron uptake. We demonstrate that ywbLMN, encoding an elemental iron uptake system orthologous to the copper oxidase-dependent Fe(III) uptake system of Saccharomyces cerevisiae, is essential for growth in low iron minimal medium lacking citric acid. 2,3-Dihydroxybenzoyl-glycine (Itoic acid), the siderophore precursor produced by laboratory strains of B. subtilis, is of secondary importance. In the presence of citrate, the YfmCDEF ABC transporter is required for optimal growth. B. subtilis is unable to grow in minimal medium containing the iron chelator EDDHA unless the ability to synthesize the intact bacillibactin siderophore is restored (by the introduction of a functional sfp gene) or exogenous siderophores are provided. Utilization of the catecholate siderophores bacillibactin and enterobactin requires the FeuABC importer and the YusV ATPase. Utilization of hydroxamate siderophores requires the FhuBGC ABC transporter together with the FhuD (ferrichrome) or YxeB (ferrioxamine) substrate-binding proteins. Growth with schizokinen or arthrobactin is at least partially dependent on the YfhA YfiYZ importer and the YusV ATPase. We have also investigated the effects of a fur mutation on the proteome and documented the derepression of 11 Fur-regulated proteins, including a newly identified thioredoxin reductase homolog, YcgT.

Vasculitis and Thrombosis due to the Sea Lion Lungworm, Parafilaroides decorus, in a Guadalupe Fur Seal ( Arctocephalus philippii townsendi).

PubMed

Seguel, Mauricio; Nadler, Steven; Field, Cara; Duignan, Padraig

2018-05-01

A free-ranging, male, yearling Guadalupe fur seal ( Arctocephalus philippii townsendi) died due to multifocal verminous vasculitis with thrombosis and several embolic infarcts in liver, kidney, and brain. Nematodes extracted from lung blood vessels were identified as Parafilaroides decorus, a parasite normally found in alveoli of California sea lions ( Zalophus californianus).

Theory of Non-First Norman Form Relational Databases

DTIC Science & Technology

1986-01-01

741. [BR] Benn, W. and B. Radig, "Erweiterte Anfragen nach Relationenge- bilden in Form nichtnormlalisierter Relationen." In Datenbank - Systeme fur...Relationenmodells." In Datenbank -Systeme far Biro, Technik und Wissenschaft, A. Blaser, P. Pistor, Eds., Informatik-Fachberichte Nr. 94, Springer...Versionenbe- hafteter, Hierarchisch Strukturierter Tupel." In Datenbank -Systeme fur Biro, Technik und Wissenschaft, A. Blaser, P. Pistor, Eds

The value of wooded draws on the northern high plains for hunting, furs, and woodcutting

Treesearch

Ardell J. Bjugstad; Cindy F. Sorg

1985-01-01

Data from wildlife habitat use, wood production, and values of hunting, trapping, and firewood reflect the contribution to values of wooded draws on the northern High Plains. Values included expenditures and net willingness to pay. Approximate values per annum derived were: deer hunting $26 million; turkey hunting $1 million; fur trapping $4 million; and firewood $7...

Oxygen Transport to Human Tissues.

DTIC Science & Technology

1981-06-01

mountain climbing. Then Dr. Luft’s scientific curiosity turned to the problems encountered by the military fliers. The effect(s) of hypoxia, decompression...instantaneous hypoxia in man. J. .4ppl. Phiwil. 8:444-454. Gioddard. R.F. and U.C.L. Intermittent positive pressure-aerosol therapy in pediatrics . Di...mood and the accompanying realization by many that the conceptual and technical advances associated with military operations in the United States and

BioRef: A versatile time-of-flight reflectometer for soft matter applications at Helmholtz-Zentrum Berlin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Strobl, M.; Kreuzer, M.; Helmholtz-Zentrum Berlin, Hahn-Meitner-Platz 1, 14109 Berlin

2011-05-15

BioRef is a versatile novel time-of-flight reflectometer featuring a sample environment for in situ infrared spectroscopy at the reactor neutron source BER II of the Helmholtz Zentrum Berlin fuer Materialien und Energie (HZB). After two years of design and construction phase the instrument has recently undergone commissioning and is now available for specular and off-specular neutron reflectivity measurements. BioRef is especially dedicated to the investigation of soft matter systems and studies at the solid-liquid interface. Due to flexible resolution modes and variable addressable wavelength bands that allow for focusing onto a selected scattering vector range, BioRef enables a broad rangemore » of surface and interface investigations and even kinetic studies with subsecond time resolution. The instrumental settings can be tailored to the specific requirements of a wide range of applications. The performance is demonstrated by several reference measurements, and the unique option of in situ on-board infrared spectroscopy is illustrated by the example of a phase transition study in a lipid multilayer film.« less

"Rejoicing in the Beauties of Nature": The Image of the Western Landscape during the Fur Trade

ERIC Educational Resources Information Center

Oman, Kerry R.

2009-01-01

While traveling along the Platte River on May 18, 1834, William Marshall Anderson stopped to pick up a human skull bleaching in the prairie sunlight. Anderson was from Louisville, Kentucky, and had been sent west by his physician to accompany a fur-trade caravan to the Rocky Mountains in hopes of regaining lost physical strength. He came west not…

Rv2358 and FurB: Two Transcriptional Regulators from Mycobacterium tuberculosis Which Respond to Zinc

PubMed Central

Canneva, Fabio; Branzoni, Manuela; Riccardi, Giovanna; Provvedi, Roberta; Milano, Anna

2005-01-01

In a previous work, we demonstrated that the Mycobacterium tuberculosis Rv2358-furB operon is induced by zinc. In this study, the orthologous genes from Mycobacterium smegmatis mc2155 were inactivated and mutants analyzed. Rv2358 protein was purified and found to bind upstream of the Rv2358 gene. Binding was inhibited by Zn2+ ions. PMID:16077132

16 CFR 301.1 - Terms defined.

Code of Federal Regulations, 2010 CFR

2010-01-01

... (approved Aug. 8, 1951, Pub. L. 110, 82d Cong., 1st Sess.; 15 U.S.C.A. sec. 69; 65 Stat. 179). (2) The terms... information as may be permitted by the regulations, when and if used. (6) The term cat fur means the pelt or... animal of the species Canis familiaris. (8) The term dog or cat fur product means any item of merchandise...

16 CFR 301.1 - Terms defined.

Code of Federal Regulations, 2014 CFR

2014-01-01

... (approved Aug. 8, 1951, Pub. L. 110, 82d Cong., 1st Sess.; 15 U.S.C.A. sec. 69; 65 Stat. 179). (2) The terms... information as may be permitted by the regulations, when and if used. (6) The term cat fur means the pelt or... animal of the species Canis familiaris. (8) The term dog or cat fur product means any item of merchandise...

16 CFR 301.1 - Terms defined.

Code of Federal Regulations, 2013 CFR

2013-01-01

... (approved Aug. 8, 1951, Pub. L. 110, 82d Cong., 1st Sess.; 15 U.S.C.A. sec. 69; 65 Stat. 179). (2) The terms... information as may be permitted by the regulations, when and if used. (6) The term cat fur means the pelt or... animal of the species Canis familiaris. (8) The term dog or cat fur product means any item of merchandise...

16 CFR 301.1 - Terms defined.

Code of Federal Regulations, 2011 CFR

2011-01-01

... (approved Aug. 8, 1951, Pub. L. 110, 82d Cong., 1st Sess.; 15 U.S.C.A. sec. 69; 65 Stat. 179). (2) The terms... information as may be permitted by the regulations, when and if used. (6) The term cat fur means the pelt or... animal of the species Canis familiaris. (8) The term dog or cat fur product means any item of merchandise...

16 CFR 301.1 - Terms defined.

Code of Federal Regulations, 2012 CFR

2012-01-01

... (approved Aug. 8, 1951, Pub. L. 110, 82d Cong., 1st Sess.; 15 U.S.C.A. sec. 69; 65 Stat. 179). (2) The terms... information as may be permitted by the regulations, when and if used. (6) The term cat fur means the pelt or... animal of the species Canis familiaris. (8) The term dog or cat fur product means any item of merchandise...

The Aggregate National Supply of Job Openings and Firms' Procedures for Filling Positions. IAB Labour Market Research Topics.

ERIC Educational Resources Information Center

Magvas, Emil; Spitznagel, Eugen

Surveys by the Institut fur Arbeitsmarkt- und Berufsforschung (IAB) of German firms' job openings have been combined with job registry data from the Bundesanstalt fur Arbeit on an annual basis since 1989 in order to determine the scope and structure of the aggregate national supply of job openings. The surveys also indicated problems encountered…

Isotopic niche partitioning between two apex predators over time.

PubMed

Drago, Massimiliano; Cardona, Luis; Franco-Trecu, Valentina; Crespo, Enrique A; Vales, Damián G; Borella, Florencia; Zenteno, Lisette; Gonzáles, Enrique M; Inchausti, Pablo

2017-07-01

Stable isotope analyses have become an important tool in reconstructing diets, analysing resource use patterns, elucidating trophic relations among predators and understanding the structure of food webs. Here, we use stable carbon and nitrogen isotope ratios in bone collagen to reconstruct and compare the isotopic niches of adult South American fur seals (Arctocephalus australis; n = 86) and sea lions (Otaria flavescens; n = 49) - two otariid species with marked morphological differences - in the Río de la Plata estuary (Argentina - Uruguay) and the adjacent Atlantic Ocean during the second half of the 20th century and the beginning of the 21st century. Samples from the middle Holocene (n = 7 fur seals and n = 5 sea lions) are also included in order to provide a reference point for characterizing resource partitioning before major anthropogenic modifications of the environment. We found that the South American fur seals and South American sea lions had distinct isotopic niches during the middle Holocene. Isotopic niche segregation was similar at the beginning of the second half of the 20th century, but has diminished over time. The progressive convergence of the isotopic niches of these two otariids during the second half of the 20th century and the beginning of the 21st century is most likely due to the increased reliance of South American fur seals on demersal prey. This recent dietary change in South American fur seals can be explained by at least two non-mutually exclusive mechanisms: (i) the decrease in the abundance of sympatric South American sea lions as a consequence of small colony size and high pup mortality resulting from commercial sealing; and (ii) the decrease in the average size of demersal fishes due to intense fishing of the larger class sizes, which may have increased their accessibility to those eared seals with a smaller mouth gape, that is, South American fur seals of both sexes and female South American sea lions. © 2017 The Authors. Journal of Animal Ecology © 2017 British Ecological Society.

Single use disposable digital flexible ureteroscopes: an ex-vivo assessment and cost analysis.

PubMed

Hennessey, D B; Fojecki, G; Papa, N; Lawrentschuk, N; Bolton, D

2018-04-15

The single use flexible ureteroscope (fURS), the LithoVue is an important recent development. We aim to measure the capability of this instrument and to assess if there is a benefit to switching to single use instruments. The LithoVue was compared to Olympus URF-V and Stortz Flex Xc ex-vivo. An analysis of reusable fURS usage was performed to evaluate damage, durability and maintenance costs. This was then compared to the projected costs of using single use instruments. Flexion, deflection and irrigation flow of the LithoVue was equivalent, if not better than reusable instruments. An analysis of 234 procedures with 7 new Olympus URF-V scopes, revealed 15 scope damages. Staghorn stones and lower pole/midzone stones were significant risk factors for damage, p=0.014. Once damage occurred, it was likely to occur again. Total repair costs were $162,628 (£92,411), the mean cost per case is $695 (£395). Factoring in the purchase cost, cleaning and repair costs, and the cumulative cost of 28 reusable fURS cases is approximately $50,000 (£28,412). If the LithoVue was priced at $1200 AUD, switching to a single use scope would cost approximately $35,000 (£19,888). The LithoVue is analogous to reusable fURS scopes in regard to standard technical metrics. Depending on its purchase cost it may also represent a cost saving for hospitals when compared to the cumulative costs of maintaining reusable fURS. Additionally, urologist may consider to use the scope in cases in which reusable scope damage is anticipated. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

Simulation of RIRS in soft cadavers: a novel training model by the Cadaveric Research On Endourology Training (CRET) Study Group.

PubMed

Huri, Emre; Skolarikos, Andreas; Tatar, İlkan; Binbay, Murat; Sofikerim, Mustafa; Yuruk, Emrah; Karakan, Tolga; Sargon, Mustafa; Demiryurek, Deniz; Miano, Roberto; Bagcioglu, Murat; Ezer, Mehmet; Cracco, Cecilia Maria; Scoffone, Cesare Marco

2016-05-01

The aim of the current study was to evaluate the use of fresh-frozen concurrently with embalmed cadavers as initial training models for flexible ureteroscopy (fURS) in a group of urologists who were inexperienced in retrograde intrarenal surgery (RIRS). Twelve urologists involved in a cadaveric fURS training course were enrolled into this prospective study. All the participants were inexperienced in fURS. Theoretical lectures and step-by-step tips and tricks video presentations on fURS were used to incorporate the technical background of the procedure to the hands-on-training course and to standardize the operating steps of the procedure. An 8-item survey was administered to the participants upon initiation and at the end of the course. Pre- and post-training scores were similar for each question. All the participants successfully completed the hands-on-training tasks. Mean pre-training duration [3.56 ± 2.0 min (range 1.21-7.46)] was significantly higher than mean post-training duration [1.76 ± 1.54 min (range 1.00-6.34)] (p = 0.008). At the end of the day, the trainers checked the integrity of the collecting system both by endoscopy and by fluoroscopy and could not detect any injury of the upper ureteral wall or pelvicalyceal structures. The functionality of the scopes was also checked, and no scope injury (including a reduction in the deflection capacity) was noted. The fURS simulation training model using soft human cadavers has the unique advantage of perfectly mimicking the living human tissues. This similarity makes this model one of the best if not the perfect simulator for an effective endourologic training.

Molecular and morphometric evidence for separate species of Uncinaria (Nematoda: Ancylostomatidae) in California sea lions and northern fur seals: hypothesis testing supplants verification.

PubMed

Nadler, S A; Adams, B J; Lyons, E T; DeLong, R L; Melin, S R

2000-10-01

California sea lions (Zalophus californianus) and northern fur seals (Callorhinus ursinus) are each believed to host distinct hookworm species (Uncinaria spp.). However, a recent morphometric analysis suggested that a single species parasitizes multiple pinniped hosts, and that the observed differences are host-induced. To explore the systematics of these hookworms and test these competing hypotheses, we obtained nucleotide sequences of nuclear ribosomal DNA (D2/D3 28S, D18/D19 28S, and internal transcribed spacer [ITS] regions) from 20 individual hookworms parasitizing California sea lion and northern fur seal pups where their breeding grounds are sympatric. Five individuals from an allopatric population of California sea lions were also sampled for ITS-1 and D18/D19 28S sequences. The 28S D2/D3 sequences showed no diagnostic differences among hookworms sampled from individual sea lions and fur seals, whereas the 28S D18/D19 sequences had one derived (apomorphic) character demarcating hookworms from northern fur seals. ITS sequences were variable for 7 characters, with 4 derived (apomorphic) states in ITS-1 demarcating hookworms from California sea lions. Multivariate analysis of morphometric data also revealed significant differences between nematodes representing these 2 host-associated lineages. These results indicate that these hookworms represent 2 species that are not distributed indiscriminately between these host species, but instead exhibit host fidelity, evolving independently with each respective host species. This evolutionary approach to analyzing sequence data for species delimitation is contrasted with similarity-based methods that have been applied to numerous diagnostic studies of nematode parasites.

Monoamine Release during Unihemispheric Sleep and Unihemispheric Waking in the Fur Seal

PubMed Central

Lyamin, Oleg I.; Lapierre, Jennifer L.; Kosenko, Peter O.; Kodama, Tohru; Bhagwandin, Adhil; Korneva, Svetlana M.; Peever, John H.; Mukhametov, Lev M.; Siegel, Jerome M.

2016-01-01

Study Objectives: Our understanding of the role of neurotransmitters in the control of the electroencephalogram (EEG) has been entirely based on studies of animals with bilateral sleep. The study of animals with unihemispheric sleep presents the opportunity of separating the neurochemical substrates of waking and sleep EEG from the systemic, bilateral correlates of sleep and waking states. Methods: The release of histamine (HI), norepinephrine (NE), and serotonin (5HT) in cortical and subcortical areas (hypothalamus, thalamus and caudate nucleus) was measured in unrestrained northern fur seals (Callorhinus ursinus) using in vivo microdialysis, in combination with, polygraphic recording of EEG, electrooculogram, and neck electromyogram. Results: The pattern of cortical and subcortical HI, NE, and 5HT release in fur seals is similar during bilaterally symmetrical states: highest in active waking, reduced in quiet waking and bilateral slow wave sleep, and lowest in rapid eye movement (REM) sleep. Cortical and subcortical HI, NE, and 5HT release in seals is highly elevated during certain waking stimuli and behaviors, such as being sprayed with water and feeding. However, in contrast to acetylcholine (ACh), which we have previously studied, the release of HI, NE, and 5HT during unihemispheric sleep is not lateralized in the fur seal. Conclusions: Among the studied neurotransmitters most strongly implicated in waking control, only ACh release is asymmetric in unihemispheric sleep and waking, being greatly increased on the activated side of the brain. Commentary: A commentary on this article appears in this issue on page 491. Citation: Lyamin OI, Lapierre JL, Kosenko PO, Kodama T, Bhagwandin A, Korneva SM, Peever JH, Mukhametov LM, Siegel JM. Monoamine release during unihemispheric sleep and unihemispheric waking in the fur seal. SLEEP 2016;39(3):625–636. PMID:26715233

Flexible ureterorenoscopy for lower pole stones: influence of the collecting system's anatomy.

PubMed

Jessen, Jan Peter; Honeck, Patrick; Knoll, Thomas; Wendt-Nordahl, Gunnar

2014-02-01

The impact of renal anatomy on the success rate of flexible ureterorenoscopy (fURS) for lower pole stones is less clear than it is on shock wave lithotripsy, for which it is a recognized influence factor. We analyzed safety and efficiency of fURS using modern endoscopes for lower pole stones dependent on the collecting system's configuration. We retrospectively evaluated a consecutive sample of 111 fURS for lower pole stones at our tertiary care center between January 2010 and September 2012 from our prospectively kept database. All procedures were performed with modern flexible ureterorenoscopes, nitinol baskets, holmium laser lithotripsy, and ureteral access sheaths whenever needed. The infundibular length (IL) and width (IW) and infundibulopelvic angle (IPA) were measured and the data were stratified for stone-free status and complications classified by the Clavien-Dindo scale. Univariate and multifactorial statistical analyses were performed. Correlation of operation time (OR-time) with anatomical parameters was conducted. Ninety-eight (88.3%) of the 111 patients were stone free after a single fURS. On multifactorial analysis, the stone size and IL had significant influence on the stone-free rate (SFR) (p<0.01), whereas IW did not. An acute IPA (<30°) also had significant influence (p=0.01). The incidence of complications and OR-time were not influenced by the pelvicaliceal anatomy. fURS is a safe and efficient treatment option for lower pole kidney stones. A long infundibulum and a very acute IPA (<30°) negatively affect the SFR. However, with second look procedures, a complete stone clearance is achievable even in case of unfavorable anatomic conditions. A narrow infundibulum has no negative effect while using modern endoscopes. The complication rate is not affected by the collecting system's anatomy.

Human Hair, Baltic Grey Seal (Halichoerus grypus) Fur and Herring Gull (Larus argentatus) Feathers as Accumulators of Bisphenol A and Alkylphenols.

PubMed

Nehring, Iga; Staniszewska, Marta; Falkowska, Lucyna

2017-05-01

The purpose of the study was to determine the concentration of bisphenol A (BPA), 4-tert-octylphenol (OP), and 4-nonylphenol (NP), in human hair, the fur of Baltic grey seals and the feathers of herring gulls. Hair was collected from 42 volunteers, while grey seal fur (n = 17) came from the seal centre in Hel (Marine Station of Institute of Oceanography, University of Gdansk) and gull covert feathers (n = 26) were collected from dead herring gulls along the Southern Baltic coast. Assays of phenol derivatives were conducted using the high performance liquid chromatography with fluorescence detection technique. In human hair, the mean BPA concentration amounted to 411.2 ng g -1  dw, OP 131.2 ng g -1  dw, NP 4478.4 ng g -1  dw, in seal fur BPA 67.5 ng g -1  dw, OP 62.8 ng g -1  dw, NP 39.1 ng g -1  dw, and in feathers BPA 145.1 ng g -1  dw, OP 162.0 ng g -1  dw, NP 37.7 ng g -1  dw. The increase of the analysed EDCs in hair was significantly influenced by diet rich in products of marine origin, as well as hair colouring, heating up food in plastic containers, using home cleaning products without protective gloves and wearing newly purchased clothes without washing them first. The concentration of phenol derivatives in seal fur was influenced solely by the uniform diet rich in fish. In birds, the feeding area during molting significantly influenced the concentration of BPA, OP and NP found in covert feathers.

Chemistry & migration mysteries: Fur holds clues to previous journeys

USGS Publications Warehouse

Cryan, Paul M.

2004-01-01

The bat was not only pregnant but downright angry as I snipped a bit of fur from her back. Within a few seconds, however, she flapped her powerful wings, took off from my hand and disappeared into the night, rejoining thousands of female hoary bats (Lasiurus cinereus) on their migration through the mountains of New Mexico.Every spring, hundreds of these expectant mothers pass through this small stream drainage on their way to birthing grounds farther east. Their annual passage was first reported here more than 30 years ago, and it is still one of the few known migration corridors in the area.My task that night was simple: catch hoary bats and snip tiny samples of fur from their thick coats, then let them continue on their way. The explanation, however, is a bit more complicated.

Points of Contact for Oceanographic Institutes in Europe and Russia

DTIC Science & Technology

1993-05-20

Wolfgang dle Initial: t Name: Fennel Title: Dr. 40ompany: Institut fur Ostseeforschung Address 1: Warnemunde an der Universitat Rostock Address 2...Phone: Cellular: Category: physics Notes: fennel~physik iowarnemuende.dbp.de, Telex: 398516 do d Wolfgang Fijnaut Royal Netherlands Meteorological...34Phone: Fax: Home Phone: Cellular: Category: administration Notes: Head Krause AifredWegenerinstitut fur Polar u4p M_.peresforechun +49 (0) 471 4831 0

The East-German Research Landscape in Transition. Part C. Research at East-German Universities

DTIC Science & Technology

1993-03-10

Software-Systemlosungen fUr Aufgaben der Qualit ~ tssicherung und Pr~zisionsmeBtechnik. Beratung zur automatisierten ProzeBsteuerung und rechnergestUtzten... Qualit ~ tssicherung Beratung zu Schnittstellenproblemen und zur Lichtzeichentechnik Beratung zu Auswahl und Einsatz von metrischen Bi...49 (351) 463-2786 with seventeen institutes. C45.wp-09 05 MAR 93 #4350 DEPARTMENT FOR CIVIL ENGINEERING. WATER- AND FOREST TECHNOLOGY Fakult~t fUr Bau

The foods of fur animals of the Patuxent Research Refuge, Maryland

USGS Publications Warehouse

Llewellyn, L.M.; Uhler, F.M.

1952-01-01

Approximately 300 digestive tracts of fur animals obtained mostly during the winter trapping season and 560 scats from animals live-trapped on the Patuxent Research Refuge near Laure!, Maryland, were analyzed. The resulting data are summarized and a brief description of the area and important habitat types is given. The animals studied include the raccoon, red fox, gray fox, mink, New York weasel, skunk, opossum, and house cat.

Lake Erie Wastewater Management Study.

DTIC Science & Technology

1983-06-01

Lake Erie water quality problem which It has been recognized for many years, dating back this program focused on may be succinctly described Ito...mechanisms fo’ detachment and less. As will be discussed , the costs of achieving fur- transport of sediment and phosphorus to the lake. Fur- ther...WETLANDS FOREST MIXED URBAN OTHER WATER TRANSPORTATION I UTILITIES MISSING are extensively grown in the Lake Erie Basin, especial- measurement by U.S

He Who Seeks Shall Find... Or Perhaps Not? Analysis of Firms' Searches for Qualified Personnel, Using Data from the IAB Establishment Panel 2000. IAB Labour Market Research Topics.

ERIC Educational Resources Information Center

Kolling, Arnd

The success of German firms' searches for qualified personnel to fill openings in skilled occupations was examined through a statistical analysis of data from the Institut fur Arbeitsmarkt- und Berufsforschung der Bundesanstalt fur Arbeit's (IAB) establishment panel for 2000. An employer search model was used to explain the current German debate…

Feline Non-repetitive Mitochondrial DNA Control Region Database for Forensic Evidence

PubMed Central

Grahn, R. A.; Kurushima, J. D.; Billings, N. C.; Grahn, J.C.; Halverson, J. L.; Hammer, E.; Ho, C.K.; Kun, T. J.; Levy, J.K.; Lipinski, M. J.; Mwenda, J.M.; Ozpinar, H.; Schuster, R.K; Shoorijeh, S.J.; Tarditi, C. R.; Waly, N.E.; Wictum, E. J.; Lyons, L. A.

2010-01-01

The domestic cat is the one of the most popular pets throughout the world. A by-product of owning, interacting with, or being in a household with a cat is the transfer of shed fur to clothing or personal objects. As trace evidence, transferred cat fur is a relatively untapped resource for forensic scientists. Both phenotypic and genotypic characteristics can be obtained from cat fur, but databases for neither aspect exist. Because cats incessantly groom, cat fur may have nucleated cells, not only in the hair bulb, but also as epithelial cells on the hair shaft deposited during the grooming process, thereby generally providing material for DNA profiling. To effectively exploit cat hair as a resource, representative databases must be established. This study evaluates 402 bp of the mtDNA control region (CR) from 1,394 cats, including cats from 25 distinct worldwide populations and 26 breeds. Eighty-three percent of the cats are represented by 12 major mitotypes. An additional 8.0% are clearly derived from the major mitotypes. Unique sequences were found in 7.5% of the cats. The overall genetic diversity for this data set was 0.8813 ± 0.0046 with a random match probability of 11.8%. This region of the cat mtDNA has discriminatory power suitable for forensic application worldwide. PMID:20457082

Flexible Ureterorenoscopy for Treatment of Kidney Stones: Establishment as Primary Standard Therapy in a Tertiary Stone Center.

PubMed

Ising, Stephan; Labenski, Heike; Baltes, Stefan; Khaffaf, Aso; Thomas, Christian; Wiesner, Christoph

2015-01-01

To analyze the primary stone free rate (pSFR) of flexible ureterorenoscopy (fURS) in the treatment of renal stones and to identify clinical predictors for the primary freedom from renal stones. Two hundred and seventy five patients, who underwent fURS for kidney stones were analyzed. Index stone size was 6 mm. The stone was located in the lower calyx in 48%. Ureteral access sheath was used in 97%. Operation time was 35 min and primary stone clearance was 83%. pSFR increased from 74% in 2012 to 83% in 2013 and 90% in 2014 (p = 0.001). Preoperative stenting, index stone size, cumulative stone size, lithotripsy, ureteral access sheath and operation time were significantly correlated with the pSFR by univariate analysis. Multivariate regression analysis showed index stone size, cumulative stone size, ureteral access sheath and operation time as independent parameters for pSFR. fURS for kidney stones is safe with a high pSFR. Clinical parameters for pSFR are stone size, use of ureteral access sheath and operation time. In future, the effective use of fURS for the removal of kidney stones needs to be checked by prospective randomized trials. © 2015 S. Karger AG, Basel.

Revisiting Caroline Furness's An Introduction to the Study of Variable Stars on its Centenary (Poster abstract)

NASA Astrophysics Data System (ADS)

Larsen, K.

2016-06-01

(Abstract only) A century and one month ago (October 1915) Dr. Caroline Ellen Furness (1869-1936), Director of the Vassar College Observatory, published An Introduction to the Study of Variable Stars. Issued in honor of the fiftieth anniversary of the founding of Vassar College, the work was meant to fill a void in the literature, namely as both an introduction to the topic of variable stars and as a manual explaining how they should be observed and the resulting data analyzed. It was judged to be one of the hundred best books written by an American woman in the last hundred years at the 1933 World's Fair in Chicago. The book covers the relevant history of and background on types of variable stars, star charts, catalogs, and the magnitude scale, then describes observing techniques, including visual, photographic, and photoelectric photometry. The work finishes with a discussion of light curves and patterns of variability, with a special emphasis on eclipsing binaries and long period variables. Furness's work is a valuable snapshot of the state of astronomical knowledge, technology, and observing techniques from a century ago. This presentation will analyze both Furness's book and its reception in the scientific community, and draw parallels to current advice given to beginning variable star observers.

In vivo DNA deletion assay to detect environmental and genetic predisposition to cancer.

PubMed

Reliene, Ramune; Bishop, Alexander J R; Aubrecht, Jiri; Schiestl, Robert H

2004-01-01

Large-scale genomic rearrangements such as DNA deletions play a role in the etiology of cancer. The frequency of DNA deletions can be elevated by exposure to carcinogens or by mutations in genes involved in the maintenance of genomic integrity. The in vivo DNA deletion assay allows a visual detection of deletion events within the pink-eyed unstable (pun) locus in developing mouse embryos. A deletion of one copy of a duplicated 70-kb DNA fragment within the pun locus restores the pink-eyed dilute (p) gene, which encodes a protein responsible for the assembly of a black color melanin complex. Deletion events occurring in premelanocytes cause visible black patches (fur-spots) on the light gray fur of offspring and black pigmented cells (eye-spots) on the unpigmented retinal pigment epithelium (RPE). In the fur-spot assay, 10-d-old pups are observed for black spots on the fur. In the eye-spot assay, mice are sacrificed at d 20, eyes are removed, and the wholemount RPE slides are prepared for eye-spot analysis. The frequency, size, and position relative to the optic nerve of the eye-spots are determined. This assay can be used to study the effect of environmental chemicals and physical agents as well as the genetic control of DNA deletions in vivo.

Optics for Advanced Neutron Imaging and Scattering

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moncton, David E.; Khaykovich, Boris

2016-03-30

During the report period, we continued the work as outlined in the original proposal. We have analyzed potential optical designs of Wolter mirrors for the neutron-imaging instrument VENUS, which is under construction at SNS. In parallel, we have conducted the initial polarized imaging experiment at Helmholtz Zentrum, Berlin, one of very few of currently available polarized-imaging facilities worldwide.

Towards Incorporating Educational Development in the Educational System; An Evolving Model in the German Federal Republique.

ERIC Educational Resources Information Center

Hinst, Klaus

The Center for Educational Technology (Bildungstechnologisches Zentrum -- BTZ) is a research, development, and implementation (R and D) organization set up by the State of Hesse, West Germany. It has the novel (for Germany) function of being a middleman between politics, research, and educational practice in developing curriculum for German pre-,…

Prevalence of fur mites in pet rabbits in South Korea.

PubMed

Kim, Sang-Hun; Jun, Hyung-Kyou; Song, Kun-Ho; Gram, Dunbar; Kim, Duck-Hwan

2008-06-01

The prevalence of fur mites, Cheyletiella parasitovorax and Leporacarus gibbus, in pet rabbits in South Korea was investigated by a diagnostic evaluation of skin surface tape strips and hair coat combings. C. parasitovorax was found in 80 of 140 rabbits (57.1%) and L. gibbus in six of 140 rabbits (4.3%). Clinical signs of pruritus and scaling were observed in 17 of 80 and 76 of 80 infested rabbits, respectively.

Virtual Reality and Online Databases: Will "Look and Feel" Literally Mean "Look" and "Feel"? [and]"Online" Interviews Dr. Thomas A. Furness III, Virtual Reality Pioneer.

ERIC Educational Resources Information Center

Miller, Carmen

1992-01-01

The first of two articles discusses virtual reality (VR) and online databases; the second one reports on an interview with Thomas A. Furness III, who defines VR and explains work at the Human Interface Technology Laboratory (HIT). Sidebars contain a glossary of VR terms and a conversation with Toni Emerson, the HIT lab's librarian. (LRW)

Structure Function Analysis of the Ferric Uptake Regulator (Fur) of Helicobacter pylori

DTIC Science & Technology

2010-03-17

Escherichia coli, Listeria monocytogenes, and Vibrio vulnificus and hemochromatosis (125). In addition, iron overload in haemodialysis patients is...transcriptional regulation of an iron-regulated virulence gene in Vibrio cholerae. Proc Natl Acad Sci U S A 88:1125-9. 89. Goodwin, C. S., J. A...via direct interaction of Fur in a pathogenic bacterium, Vibrio vulnificus. J Bacteriol 189:2629-36. 76 130. Lee, H. J., K. J. Park, A. Y

Adamantyl- and Furanyl-Protected Nanoscale Silver Sulfide Clusters.

PubMed

Bestgen, Sebastian; Yang, Xiaoxun; Issac, Ibrahim; Fuhr, Olaf; Roesky, Peter W; Fenske, Dieter

2016-07-11

The silver salts of 1-adamantanethiol (AdSH) and furan-2-ylmethanethiol (FurCH2 SH) were successfully applied as building blocks for ligand-protected Ag2 S nanoclusters. The reaction of the silver thiolates [AgSAd]x and [AgSCH2 Fur]x with S(SiMe3 )2 and 1,5-bis(diphenylphosphino)pentane (dpppt) afforded three different clusters with 58, 94 and, 190 silver atoms. The intensely colored compounds [Ag58 S13 (SAd)32 ] (1), [Ag94 S34 (SAd)26 (dpppt)6 ] (2), and [Ag190 S58 (SCH2 Fur)74 (dpppt)8 ] (3) were structurally characterized by single-crystal X-ray diffraction and exhibit different cluster core geometries and ligand shells. The diameters of the well-defined sphere-shaped nanoclusters range from 2.2 nm to 3.5 nm. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

In-house zinc SAD phasing at Cu Kα edge.

PubMed

Kim, Min-Kyu; Lee, Sangmin; An, Young Jun; Jeong, Chang-Sook; Ji, Chang-Jun; Lee, Jin-Won; Cha, Sun-Shin

2013-07-01

De novo zinc single-wavelength anomalous dispersion (Zn-SAD) phasing has been demonstrated with the 1.9 Å resolution data of glucose isomerase and 2.6 Å resolution data of Staphylococcus aureus Fur (SaFur) collected using in-house Cu Kα X-ray source. The successful in-house Zn-SAD phasing of glucose isomerase, based on the anomalous signals of both zinc ions introduced to crystals by soaking and native sulfur atoms, drove us to determine the structure of SaFur, a zinc-containing transcription factor, by Zn-SAD phasing using in-house X-ray source. The abundance of zinc-containing proteins in nature, the easy zinc derivatization of the protein surface, no need of synchrotron access, and the successful experimental phasing with the modest 2.6 Å resolution SAD data indicate that inhouse Zn-SAD phasing can be widely applicable to structure determination.

Development of Fracture Mechanics Maps for Composite Materials. Volume 3.

DTIC Science & Technology

1985-12-01

RD-At69 W4 DEVELOPMENT OF FRCTURE ECHNICS NPS FOR COMPOSITE V3jMATERIALS VOLUME 3( ) DEUTSCHE FORSCHUNOS- UND YERSUCHSANSTALT FUER LUFT- UND RAUMF...DEVELOPMENT OF FRACTURE MECHANICS MAPS I FOR COMPOSITE MATERIALS Dr. H. W. Bergmann DFVLR - Institute for Structural Mechanics Braunschweig, West Germany...Brussels, Belgium ELEMENT NO . NO. NO. NO. 11. TITLE (Include Security Classification) Development of N/A N/A N/A N/A Fracture MechanicsMaps for Composite

European Conference on Visual Perception (6th).

DTIC Science & Technology

1983-11-30

Institut fuer Verhaltenswissenschaft, ETH- Zentrum, 8092 Zuerich - Switzerland W. GERBINO: Istituto di Psicologia , Universitk di Padova, Piazza Capitaniato...fuer medizinische Psychologie, Schillerstrasse 42 - 8000 Muenchen 2, West Germany L. JANEZ ESCALADA: Psicologia Matem&tica, Facultad de Psicologla...Norway P. MANCINI: Istituto di Fisiologia clinica del C.N.R., Via Bonanno Pisano - 56100 Pisa, Italy C.A. MARZI: Istituto di Psicologia . Universit

Entanglement of Australian sea lions and New Zealand fur seals in lost fishing gear and other marine debris before and after Government and industry attempts to reduce the problem.

PubMed

Page, Brad; McKenzie, Jane; McIntosh, Rebecca; Baylis, Alastair; Morrissey, Adam; Calvert, Norna; Haase, Tami; Berris, Mel; Dowie, Dave; Shaughnessy, Peter D; Goldsworthy, Simon D

2004-07-01

In recent years, Australian governments and fishing industry associations have developed guiding principles aimed at reducing the impact of fishing on non-target species and the benthos and increasing community awareness of their efforts. To determine whether they reduced seal entanglement in lost fishing gear and other marine debris, we analysed Australian sea lion and New Zealand fur seal entanglement data collected from Kangaroo Island, South Australia. Contrary to our expectations, we found that entanglement rates did not decrease in recent years. The Australian sea lion entanglement rate (1.3% in 2002) and the New Zealand fur seal entanglement rate (0.9% in 2002) are the third and fourth highest reported for any seal species. Australian sea lions were most frequently entangled in monofilament gillnet that most likely originated from the shark fishery, which operates in the region where sea lions forage--south and east of Kangaroo Island. In contrast, New Zealand fur seals were most commonly entangled in loops of packing tape and trawl net fragments suspected to be from regional rock lobster and trawl fisheries. Based on recent entanglement studies, we estimate that 1478 seals die from entanglement each year in Australia. We discuss remedies such as education programs and government incentives that may reduce entanglements.

The Iron-Dependent Regulator Fur Controls Pheromone Signaling Systems and Luminescence in the Squid Symbiont Vibrio fischeri ES114

PubMed Central

Septer, Alecia N.; Lyell, Noreen L.

2013-01-01

Bacteria often use pheromones to coordinate group behaviors in specific environments. While high cell density is required for pheromones to achieve stimulatory levels, environmental cues can also influence pheromone accumulation and signaling. For the squid symbiont Vibrio fischeri ES114, bioluminescence requires pheromone-mediated regulation, and this signaling is induced in the host to a greater extent than in culture, even at an equivalent cell density. Our goal is to better understand this environment-specific control over pheromone signaling and bioluminescence. Previous work with V. fischeri MJ1 showed that iron limitation induces luminescence, and we recently found that ES114 encounters a low-iron environment in its host. Here we show that ES114 induces luminescence at lower cell density and achieves brighter luminescence in low-iron media. This iron-dependent effect on luminescence required ferric uptake regulator (Fur), which we propose influences two pheromone signaling master regulators, LitR and LuxR. Genetic and bioinformatic analyses suggested that under low-iron conditions, Fur-mediated repression of litR is relieved, enabling more LitR to perform its established role as an activator of luxR. Interestingly, Fur may similarly control the LitR homolog SmcR of Vibrio vulnificus. These results reveal an intriguing regulatory link between low-iron conditions, which are often encountered in host tissues, and pheromone-dependent master regulators. PMID:23315731

Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120.

PubMed

Sein-Echaluce, Violeta C; González, Andrés; Napolitano, Mauro; Luque, Ignacio; Barja, Francisco; Peleato, M Luisa; Fillat, María F

2015-06-01

Iron and zinc are necessary nutrients whose homeostasis is tightly controlled by members of the ferric uptake regulator (FUR) superfamily in the cyanobacterium Anabaena sp. PCC7120. Although the link between iron metabolism and oxidative stress management is well documented, little is known about the connection between zinc homeostasis and the oxidative stress response in cyanobacteria. Zinc homeostasis in Anabaena is controlled by Zur, also named FurB. When overexpressed in Escherichia coli, Zur (FurB) improved cell survival during oxidative stress. In order to investigate the possible correlation between Zur and the oxidative stress response in Anabaena, zur deletion and zur-overexpressing strains have been constructed, and the consequences of Zur imbalance evaluated. The lack of Zur increased sensitivity to hydrogen peroxide (H2 O2 ), whereas an excess of Zur enhanced oxidative stress resistance. Both mutants displayed pleiotropic phenotypes, including alterations on the filament surfaces observable by scanning electron microscopy, reduced content of endogenous H2 O2 and altered expression of sodA, catalases and several peroxiredoxins. Transcriptional and biochemical analyses unveiled that the appropriate level of Zur is required for proper control of the oxidative stress response and allowed us to identify major antioxidant enzymes as novel members of the Zur regulon. © 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.

Geophysical surveys and archaeological insights at Fort Pierre Chouteau, a frontier trading post on the Middle Missouri

NASA Astrophysics Data System (ADS)

Patton, Margaret Maurine

Fort Pierre Chouteau in present day South Dakota was the most important fur trading post of the American Fur Company in the 1830s, serving as a regional hub for the fur trade. The Fort was sold to the U.S. Military in 1855 for use as a base in the Sioux Wars but was abandoned in 1856. Geophysical surveys and previous excavations indicate evidence of both occupations. Geophysics is an important tool for determining the extent of archaeological sites, yet the relationships between geophysical anomalies and excavation features may not be readily evident. Initial geophysical surveys (Kvamme 2007) were completed to determine the extent of the fur trading Fort, and additional surveys in August 2012 used magnetometry and electrical resistance to determine if evidence of military structures exists outside of the Fort. This study examines connections between excavation features and geophysical anomalies in order to better interpret anomalies inside the Fort palisade. The palisade builder's trench, adobe pavement, post holes, and unknown structures are characterized through the analysis of the excavations and anomalies. The location of one of the military structures outside of the palisade is also identified. As many sites have histories of excavations prior to any geophysical surveys, combining the two sets of information is important in order to more fully understand site layout and the archaeological causes of geophysical anomalies.

Continental drilling for paleoclimatic records: Recommendations from an international workshop

USGS Publications Warehouse

Colman, Steve M.

1995-01-01

The Workshop, entitled "Continental Drilling for Paleoclimate Records", was sponsored by the Past Global Changes (PAGES) Project, a core project of the International Geosphere-Biosphere Programme (IGBP) and by the GeoForschungsZentrum, Potsdam, Germany, in conjunction with the International Continental Drilling Programme (ICDP). The impetus for the meeting was the need for long continental paleoclimate records that will fill gaps left by the marine and ice-core records and provide information on time and spatial scales that are relevant to human activities. Further impetus came from a perceived need to balance the forecasts and reconstructions of climate models with information on actual behavior of the climate system on the continents. The meeting was organized by Steven M. Colman, Suzanne A.G. Leroy, and Jörg F.W. Negendank and was held at the GeoForschungsZentrum, Potsdam, Germany, June 30-July 2, 1995. Because the Workshop was primarily a working meeting, a relatively small number of participants were invited (Appendix 3). Leaders of the PAGES Pole-Equator-Pole (PEP) transects and existing large-lake drilling programs, along with a mixture of technical experts, were the primary group of attendees.

Role of flexible uretero-renoscopy in management of renal calculi in anomalous kidneys: single-center experience.

PubMed

Singh, Abhishek Gajendra; Chhabra, Jaspreet Singh; Sabnis, Ravindra; Ganpule, Arvind; Jairath, Ankush; Shah, Darshan; Desai, Mahesh

2017-02-01

Flexible uretero-renoscopy (FURS) is an accepted modality for management of renal calculi in orthotopically placed kidney. Though it has been used in management of calculi in anomalous kidneys, the literature is scarce. To define the role of FURS in the management of stones in anomalous kidneys. We performed a retrospective analysis of all the patients with anomalous kidneys who primarily underwent FURS from January 2010 to December 2015 at our institute. In our study, we included patients with anomalies of lie, fusion and rotation. A total of twenty-five patients with twenty-five renal units having renal calculi in anomalous kidneys were evaluated. Indications for FURS included stone size less than or equal to 2 cm, contraindication to PCNL like bleeding tendencies, patients on anticoagulants or patients who refused ESWL and PCNL. Complete clearance of stone was defined as no residual fragment greater than 2 mm at the end of 4 weeks. The parameters evaluated were patient demographics, type of renal anomaly, stone size, location, laterality, patient's presentation, need for preoperative stenting, operative time, need for postoperative DJ stent, hospital stay, analgesic requirement, number of stages or auxiliary procedures required for stone clearance, success rate and complications. Twenty-five patients with calculi in anomalous kidneys were managed with FURS. These 25 patients had a total of 37 stones. Out of 25 patients, 14 had ectopic kidneys with 19 stones, 5 had malrotated kidneys with 6 stones, 5 had horseshoe kidneys with 11 stones and one had a left-to-right crossed fused ectopia with a single stone. Average age of presentation was 38.28 ± 12.59 years. Majority of the patients had the stones located in pelvis (n = 11) or lower calyx (n = 11). Eight stones were in middle calyx (n = 8), five in upper calyx (n = 5) and two in upper ureter (n = 2). Fifteen patients had a single stone, and 10 of them had 2 or more stones. Average size of stone was 14.71 ± 4.11 mm and average density being 1210.8 ± 237.7 Hounsfield units. Five patients had a preplaced DJ stent. Average Operative time was 74 ± 21.2 min, and patients had an average hospital stay of 59.48 ± 17.8 h. DJ stent was placed postoperatively in 21 patients, and four were managed with a ureteric catheter. Complete clearance was achieved in 22 (88 %) patients, three patients required two stages and one required the third stage. Three patients (12 %) could not be managed with FURS and required percutaneous stone clearance. Primary FURS is an effective and less invasive modality for management of renal calculi less than 2 cm in kidneys with anomalies of lie, fusion and rotation. It can offset the low clearance rate and high complication rate of ESWL and PCNL, respectively. Ureteral access sheath is an important tool to overcome anatomical challenges of anomalous kidney. Basket and Laser are indispensable accessories for FURS in anomalous kidneys.

Defensive aggregation (huddling) in Rattus norvegicus toward predator odor: individual differences, social buffering effects and neural correlates.

PubMed

Bowen, Michael T; Kevin, Richard C; May, Matthew; Staples, Lauren G; Hunt, Glenn E; McGregor, Iain S

2013-01-01

Aggregation is a defensive strategy employed by many prey species in response to predatory threat. Our group has characterized defensive aggregation (huddling) in Rattus norvegicus in response to a ball of cat fur. In this situation some rats huddle less, and approach the threatening cue more than others (active vs. passive responders). The present study explored whether active responding is a stable phenotype associated with behaviors outside direct predatory encounters. The neural substrates of active and passive responding under predatory threat were explored using c-Fos immunohistochemistry. Finally, we examined whether the presence of conspecifics during predatory threat biases behavior towards active responding. Active and passive responding styles were found to be stable in individual rats across consecutive group exposures to cat fur, and were predicted by anxiety-like behavior in an open-field emergence test. Active responders displayed less conditioned fear in an environment associated with predatory threat, and had higher post-exposure intake of a weak sucrose solution (a test of "anhedonia"). Active responding was associated with: greater cat fur-induced activation of the accessory olfactory bulb, reflecting greater olfactory stimulation in rats actively approaching the fur; lowered activation of somatosensory cortex, reflecting reduced huddling with conspecifics; and reduced activation in the lateral septum. Social exposure to cat fur promoted active responding relative to individual exposure, and lowered c-Fos expression in the dorsomedial periaqueductal grey, medial caudate putamen and lateral habenula. We conclude that individual differences in anti-predator behavior appear stable traits with active responders having a more resilient phenotype. Social exposure to predatory threat has an acute buffering effect, subtly changing the neural and behavioral response towards threat and encouraging active responding. An association between active responding and lower c-Fos expression in the lateral septum is consistent with previous studies that highlight this region as an important neurobiological substrate of defensive aggregation.

Tailored minimally invasive management of complex calculi in horseshoe kidney.

PubMed

Ding, Jie; Zhang, Yuanyuan; Cao, Qifeng; Huang, Tao; Xu, Wei; Huang, Kai; Fang, Jing; Bai, Qiang; Qi, Jun; Huang, Yunteng

2015-01-01

Complex calculi in horseshoe kidney (HK) present a significant management challenge. Here, we report the clinical efficacy of extracorporeal shock wave lithotripsy (ESWL), minimally invasive percutaneous nephrolithotomy (MPCNL) and flexible ureteroscopy (FURS), combined with holmium laser lithotripsy, in the treatment of calculi in HK. From January 2005 to May 2014, 62 HK patients with renal calculi were reviewed in terms of medical history, treatment modality and therapeutic outcome in a single tertiary care hospital. Among the patients, 11 with a solitary stone ≤ 1.5 cm in diameter received ESWL, leading to overall stone-free rate of 72.7%; 18 with stone diameter ≤ 2-3 cm received retrograde flexible ureteroscopy, with a recorded mean digitized surface area (DSA) of 339.6 ± 103.9 mm2, mean operation time of 93.1 ± 11.5 minutes and overall stone-free rate of 88.9%; and 33 with staghorn or complex calculi (d ≥ 2 cm) had MPCNL or MPCNL-FURS, with a recorded mean DSA of 691.0 ± 329.9 vs. 802.9 ± 333.3 mm2, mean operation time of 106.4 ± 16.6 vs. 124.4 ± 15.1 min and overall stone-free rate of 89.5% vs. 92.9%. For complex calculi (d ≥ 2 cm), MPCNL combined with antegrade FURS was superior in terms of reducing number of tracts, controlling mean hemoglobin drop, but required longer operation time, comparing with MPCNL alone. As minimally invasive treatments, a combination of MPCNL and antegrade FURS provides a safe and effective modality in the management of staghorn or complex calculi (d ≥ 2 cm) in HK with significantly reduced blood loss comparing to MPCNL alone, and retrograde FURS alone is favorable for stones with a diameter ≤ 2-3 cm. ESWL is effective for viable small solitary stones (d ≤ 1.5 cm). Treatment modality should be tailored based on individual condition.

Iron is a signal for Stenotrophomonas maltophilia biofilm formation, oxidative stress response, OMPs expression, and virulence

PubMed Central

García, Carlos A.; Alcaraz, Eliana S.; Franco, Mirta A.; Passerini de Rossi, Beatriz N.

2015-01-01

Stenotrophomonas maltophilia is an emerging nosocomial pathogen. In many bacteria iron availability regulates, through the Fur system, not only iron homeostasis but also virulence. The aim of this work was to assess the role of iron on S. maltophilia biofilm formation, EPS production, oxidative stress response, OMPs regulation, quorum sensing (QS), and virulence. Studies were done on K279a and its isogenic fur mutant F60 cultured in the presence or absence of dipyridyl. This is the first report of spontaneous fur mutants obtained in S. maltophilia. F60 produced higher amounts of biofilms than K279a and CLSM analysis demonstrated improved adherence and biofilm organization. Under iron restricted conditions, K279a produced biofilms with more biomass and enhanced thickness. In addition, F60 produced higher amounts of EPS than K279a but with a similar composition, as revealed by ATR-FTIR spectroscopy. With respect to the oxidative stress response, MnSOD was the only SOD isoenzyme detected in K279a. F60 presented higher SOD activity than the wt strain in planktonic and biofilm cultures, and iron deprivation increased K279a SOD activity. Under iron starvation, SDS-PAGE profile from K279a presented two iron-repressed proteins. Mass spectrometry analysis revealed homology with FepA and another putative TonB-dependent siderophore receptor of K279a. In silico analysis allowed the detection of potential Fur boxes in the respective coding genes. K279a encodes the QS diffusible signal factor (DSF). Under iron restriction K279a produced higher amounts of DSF than under iron rich condition. Finally, F60 was more virulent than K279a in the Galleria mellonella killing assay. These results put in evidence that iron levels regulate, likely through the Fur system, S. maltophilia biofilm formation, oxidative stress response, OMPs expression, DSF production and virulence. PMID:26388863

Intestinal helminth fauna of the South American sea lion Otaria flavescens and fur seal Arctocephalus australis from northern Patagonia, Argentina.

PubMed

Hernández-Orts, J S; Montero, F E; Juan-García, A; García, N A; Crespo, E A; Raga, J A; Aznar, F J

2013-09-01

We report on the intestinal helminth fauna of 56 South American sea lions, Otaria flavescens, and 5 South American fur seals, Arctocephalus australis, from northern Patagonia, Argentina. A total of 97,325 helminth specimens were collected from sea lions. Gravid individuals were represented by 6 species of parasites: 1 digenean (Ascocotyle (Ascocotyle) patagoniensis), 1 cestode (Diphyllobothrium spp.), 3 nematodes (Uncinaria hamiltoni, Contracaecum ogmorhini s.s., Pseudoterranova cattani) and 1 acanthocephalan (Corynosoma australe). In addition, third-stage larvae of 2 nematodes (Contracaecum sp. and Anisakis sp. type I) and 3 juvenile acanthocephalans (Andracantha sp., Profilicollis chasmagnathi and Corynosoma cetaceum) were also collected. Andracantha sp., C. ogmorhini s.s. and P. chasmagnathi represent new host records. A total of 1516 helminth specimens were collected from fur seals. Gravid individuals were represented by three species of parasites, namely, Diphyllobothrium spp., C. ogmorhini s.s. and C. australe. In addition, larvae of Contracaecum sp. and P. cattani, juveniles of C. cetaceum and immature cestodes (Tetrabothriidae gen. sp.) were also collected. Corynosoma australe was the most prevalent and abundant parasite in both hosts, accounting for >90% of all specimens. Sea lions and furs seals from northern Patagonia harbour the intestinal helminth communities that could be predicted for otariids, i.e. the combination of species of the genera Corynosoma, Diphyllobothrium, Pseudoterranova, Contracaecum and, in pups, Uncinaria. Additionally, both species of otariid are apparently unsuitable hosts (i.e. non-hosts) for as many as five parasite taxa. The inclusion or exclusion of these species affects estimation of species richness at both component community (11 versus 6 species in sea lions; 7 versus 3 species in fur seals) and infracommunity (mean: 3.1 versus 2.6 in sea lions; 2.2 versus 1.7 species) levels. Information about the reproductive status of helminth species is often lacking in parasitological surveys on otariids and other marine vertebrates, but it is of significance to improve precision in parascript studies or ecological meta-analyses.

Pet fur or fake fur? A forensic approach

PubMed Central

2014-01-01

Background In forensic science there are many types of crime that involve animals. Therefore, the identification of the species has become an essential investigative tool. The exhibits obtained from such offences are very often a challenge for forensic experts. Indeed, most biological materials are traces, hair or tanned fur. With hair samples, a common forensic approach should proceed from morphological and structural microscopic examination to DNA analysis. However, the microscopy of hair requires a lot of experience and a suitable comparative database to be able to recognize with a high degree of accuracy that a sample comes from a particular species and then to determine whether it is a protected one. DNA analysis offers the best opportunity to answer the question, ‘What species is this?’ In our work, we analyzed different samples of fur coming from China used to make hats and collars. Initially, the samples were examined under a microscope, then the mitochondrial DNA was tested for species identification. For this purpose, the genetic markers used were the 12S and 16S ribosomal RNA, while the hypervariable segment I of the control region was analyzed afterwards, to determine whether samples belonged to the same individual. Results Microscopic examination showed that the fibres were of animal origin, although it was difficult to determine with a high degree of confidence which species they belonged to and if they came from a protected species. Therefore, DNA analysis was essential to try to clarify the species of these fur samples. Conclusions Macroscopic and microscopic analysis confirmed the hypothesis regarding the analyzed hair belonging to real animals, although it failed to prove with any kind of certainty which actual family it came from, therefore, the species remains unknown. Sequence data analysis and comparisons with the samples available in GenBank showed that the hair, in most cases, belonged to the Canidae family, and in one case only to Felidae. PMID:24991403

Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7

PubMed Central

Arenas-Hernández, Margarita M.P.; Rojas-López, Maricarmen; Medrano-López, Abraham; Nuñez-Reza, Karen J.; Puente, José Luis; Martínez-Laguna, Ygnacio; Torres, Alfredo G.

2014-01-01

The molecular mechanisms controlling expression of the Long Polar Fimbriae 2 (Lpf2) of enterohemorrhagic E. coli (EHEC) O157:H7 were evaluated. Primer extension was used to locate the lpfA2 transcriptional start site in EHEC strain EDL933 at 171 bp upstream of the lpfA2 start codon. Semi-quantitative RT-PCR demonstrated that the highest lpfA2 expression occurs between an OD600 of 1.0 and 1.2 in DMEM at pH 6.5 and 37°C. The level of lpfA2 transcription at OD600 1.2 and pH 6.5 was 4-times greater than that at pH 7.2. Although lpfA2 expression was decreased under iron-depleted conditions, its expression was increased in a Ferric-uptake-regulator (Fur) mutant strain. The lpfA2 transcript was 0.7 and 2-times more abundant in wt EHEC grown in DMEM pH 6.5 plus iron and MacConkey broth at 25°C, respectively, than in DMEM at pH 6.5. The lpf2 expression in DMEM pH 6.5 plus iron and bile salts was 2.7-times more abundant and similar to MacConkey. Further, transcription in the EDL933Δfur was 0.6 and 0.8-times higher as compared to the wt strain grown in DMEM pH 6.5 plus iron and MacConkey broth, respectively. Electrophoretic mobility shift assays (EMSA) showed that purified Fur interacts with the lpf2 regulatory region, indicating that Fur-repression is exerted by direct binding to the promoter region. In summary, we demonstrated that the EHEC lpf2 operon is regulated in response to temperature, pH, bile salts and iron, during exponential phase of growth, and controlled by Fur. PMID:24966050

A PerR-like protein involved in response to oxidative stress in the extreme bacterium Deinococcus radiodurans

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liu, Chengzhi; Wang, Liangyan; Li, Tao

2014-07-18

Highlights: • We report a novel PerR-like protein of Fur family in D. radiodurans that is not annotated in the current database. • drperR responses to H{sub 2}O{sub 2} and functions as a negative regulator of katE and dps. • We provided implications on how to utilize sequenced genome data and the importance of genome data mining. • This study adds knowledge to complicated regulatory network that responds to ROS stress in D. radiodurans. - Abstract: Response and defense systems against reactive oxygen species (ROS) contribute to the remarkable resistance of Deinococcus radiodurans to oxidative stress induced by oxidants ormore » radiation. However, mechanisms involved in ROS response and defense systems of D. radiodurans are not well understood. Fur family proteins are important in ROS response. Only a single Fur homolog is predicted by sequence similarity in the current D. radiodurans genome database. Our bioinformatics analysis demonstrated an additional guanine nucleotide in the genome of D. radiodurans that is not in the database, leading to the discovery of another Fur homolog DrPerR. Gene disruption mutant of DrPerR showed enhanced resistance to hydrogen peroxide (H{sub 2}O{sub 2}) and increased catalase activity in cell extracts. Real-time PCR results indicated that DrPerR functions as a repressor of the catalase gene katE. Meanwhile, derepression of dps (DNA-binding proteins from starved cells) gene under H{sub 2}O{sub 2} stress by DrPerR point to its regulatory role in metal ions hemostasis. Thus, DrPerR might function as a Fur homolog protein which is involved in ROS response and defense. These results help clarify the complicated regulatory network that responds to ROS stress in D. radiodurans.« less

The fat and the furriest: morphological changes in harp seal fur with ontogeny.

PubMed

Gmuca, Natalia V; Pearson, Linnea E; Burns, Jennifer M; Liwanag, Heather E M

2015-01-01

Ontogenetic changes in physiological performance often exemplify the development of adaptations to environmental challenges. For mammals in polar regions, the extreme cold of the environment presents a constant challenge to thermal homeostasis. The harp seal (Pagophilus groenlandicus) is an Arctic species that shifts its thermoregulatory strategy with ontogeny. Adult harp seals primarily use blubber for insulation, but newborn harp seals instead rely on their fur coat while their blubber layer develops. Harp seal pups are weaned abruptly, less than 2 wk after birth, and must subsequently learn to swim and dive in frigid waters on their own. This study examined how the morphological characteristics of harp seal fur change with ontogeny. We compared hair length, hair circularity, and hair density for neonates (1 d old; n = 7), early-nursing pups (4 d old; n = 3), late-nursing pups (9 d old; n = 4), newly weaned (molting) pups (2 wk old; n = 5), late-weaned (molted) pups (3 wk old; n = 4), and adult harp seals (n = 4). Hairs were shorter (P < 0.001) and flatter (P < 0.001) in older animals. Additionally, hair density decreased with age (P < 0.001), in terms of both the average number of hair bundles per unit area and the average number of underhairs present in any given bundle. These morphological changes were associated with a reduced thermal resistance of the pelt in late-weaned (molted) pups and adults (P < 0.001). Results are consistent with known evolutionary patterns of fur morphology associated with the transition from fur to blubber in aquatic species, yet this is the first time such morphological differences have been demonstrated across age classes within a single species. Thus, the ontogenetic patterns described here for harp seals recapitulate the convergent phylogenetic patterns observed across secondarily aquatic species. Overall, the timing of these ontogenetic changes may limit the ability of harp seals to adapt to the deterioration of sea ice in the Arctic, as predicted with continued climate change.

Defensive Aggregation (Huddling) in Rattus Norvegicus toward Predator Odor: Individual Differences, Social Buffering Effects and Neural Correlates

PubMed Central

Bowen, Michael T.; Kevin, Richard C.; May, Matthew; Staples, Lauren G.; Hunt, Glenn E.; McGregor, Iain S.

2013-01-01

Aggregation is a defensive strategy employed by many prey species in response to predatory threat. Our group has characterized defensive aggregation (huddling) in Rattus norvegicus in response to a ball of cat fur. In this situation some rats huddle less, and approach the threatening cue more than others (active vs. passive responders). The present study explored whether active responding is a stable phenotype associated with behaviors outside direct predatory encounters. The neural substrates of active and passive responding under predatory threat were explored using c-Fos immunohistochemistry. Finally, we examined whether the presence of conspecifics during predatory threat biases behavior towards active responding. Active and passive responding styles were found to be stable in individual rats across consecutive group exposures to cat fur, and were predicted by anxiety-like behavior in an open-field emergence test. Active responders displayed less conditioned fear in an environment associated with predatory threat, and had higher post-exposure intake of a weak sucrose solution (a test of “anhedonia”). Active responding was associated with: greater cat fur-induced activation of the accessory olfactory bulb, reflecting greater olfactory stimulation in rats actively approaching the fur; lowered activation of somatosensory cortex, reflecting reduced huddling with conspecifics; and reduced activation in the lateral septum. Social exposure to cat fur promoted active responding relative to individual exposure, and lowered c-Fos expression in the dorsomedial periaqueductal grey, medial caudate putamen and lateral habenula. We conclude that individual differences in anti-predator behavior appear stable traits with active responders having a more resilient phenotype. Social exposure to predatory threat has an acute buffering effect, subtly changing the neural and behavioral response towards threat and encouraging active responding. An association between active responding and lower c-Fos expression in the lateral septum is consistent with previous studies that highlight this region as an important neurobiological substrate of defensive aggregation. PMID:23922655

Investigating trophic relationships of pinnipeds in Alaska and Washington using stable isotope ratios of nitrogen and carbon

USGS Publications Warehouse

Hobson, Keith A.; Sease, John L.; Merrick, Richard L.; Piatt, John F.

1997-01-01

We measured stable-nitrogen (δ15N) and stable-carbon (δ13C) isotope ratios in muscle and hair from 7 northern fur seals (Callorhinus ursinus) from the Pribilof Islands, Alaska, and 27 Steller sea lions (Eumetopias jubatus), and 14 harbor seals (Phoca vitulina) from the Gulf of Alaska and coast of Washington State, in order to contrast dietary information derived from isotopic vs. available conventional dietary studies. Stable-nitrogen-isotope analysis of muscle revealed that harbor seals were enriched over sea lions (mean δ15N = 18.6‰vs. 17.5‰) which were in turn enriched over northern fur seals (mean δ15N = 16.6‰). Trophic segregation among these species likely results primarily from differential reliance on herring (Clupea harengus), Atka mackerel (Pleurogrammus monopterygius), and large vs. small walleye pollock (Theregra chalcogramma). According to their δ15N values, adult male Steller sea lions showed a higher trophic position than adult females (mean δ15N: 18.0‰ vs. 17.2‰), whereas adult female northern fur seals were trophically higher than juvenile male fur seals (mean δ15N: 16.5‰vs. 15.0‰). Each of these observed differences likely resulted from differential reliance on squid or differences in the size range of pollock consumed. Three northern fur seal pups showed higher δ15N enrichment over adults (mean 17.7‰vs. 15.8‰) due to their reliance on their mother's milk. Stable-carbon isotope measurements of hair revealed a cline toward more negative values with latitude. Segregation in hair δ13C between Steller sea lions and harbor seals off the coast of Washington (mean δ13C: -13.6‰ vs. -15.0‰) reflected the greater association of harbor seals with freshwater input from the Columbia River. Our study demonstrates the utility of the stable isotope approach to augment conventional dietary analyses of pinnipeds and other marine mammals.

DFVLR/FAA (Deutsche Forschungs-und Versuchsanstalt fuer Luft und Raumfahrt/Federal Aviation Administration) Propeller Noise Tests in the German- Dutch Wind Tunnel DNW

DTIC Science & Technology

1986-01-01

8 2.2 Test-rig Installation ................................ 9 2.3 Test Propellers ...................................... 9 2.4 In-flow...considerations, the maximUm power con- sumption of each propeller had to be limited to approximately 9 250 kW. Propeller-tip geometries as well as the radial...in the streamwise direction and arranged in a "helical" manner around a streamwise-orientated main carrier-tibe. This construct. - n concept (Fig. 9

The Helicobacter pylori Ferric Uptake Regulator (Fur) is Essential for Growth Under Sodium Chloride Stress

PubMed Central

Gancz, Hanan; Merrell, D. Scott

2011-01-01

Epidemiological data and animal models indicate that Helicobacter pylori and dietary NaCl have a synergistic ill effect on gastric maladies. Here we show that the Ferric Uptake Regulator (Fur), which is a crucial regulatory factor required for H. pylori colonization, is essential for growth in the presence of high NaCl concentrations. Moreover, we demonstrate that the transcriptional response induced by sodium chloride stress exhibits similarities to that seen under iron depletion. PMID:21538253

Effects of furan derivatives on biohydrogen fermentation from wet steam-exploded cornstalk and its microbial community.

PubMed

Liu, Zhidan; Zhang, Chong; Wang, Linjun; He, Jianwei; Li, Baoming; Zhang, Yuanhui; Xing, Xin-Hui

2015-01-01

Understanding the role of furan derivatives, furfural (FUR) and 5-hydroxymethyl furfural (HMF), is important for biofuel production from lignocellulosic biomass. In this study, the effects of furan derivatives on hydrogen fermentation from wet steam-exploded cornstalk were investigated. The control experiments with only seed sludge indicated that HMF addition of up to 1g/L stimulated hydrogen production. Similar results were obtained using steam-exploded cornstalk as the feedstock. Hydrogen productivity was increased by up to 40% with the addition of HMF. In addition, over 90% of furan derivatives with an initial concentration below 1g/L were degraded. Pyosequencing showed that the addition of HMF and FUR resulted in different microbial communities. HMF led to a higher proportion of the genera Clostridium and Ruminococcaceae, supporting the increased hydrogen production. This study suggested that hydrogen fermentation could be a detoxifying step for steam-exploded cornstalk, and HMF and FUR exhibited different functions for hydrogen fermentation. Copyright © 2014 Elsevier Ltd. All rights reserved.

Regional differences in plastic ingestion among Southern Ocean fur seals and albatrosses.

PubMed

Ryan, Peter G; de Bruyn, P J Nico; Bester, Marthán N

2016-03-15

We provide data on regional differences in plastic ingestion for two Southern Ocean top predators: Arctocephalus fur seals and albatrosses (Diomedeidae). Fur seals breeding on Macquarie Island in the 1990s excreted small (mainly 2-5 mm) plastic fragments, probably derived secondarily from myctophid fish. No plastic was found in the scats of these seals breeding on three islands in the southwest Indian and central South Atlantic Oceans, despite myctophids dominating their diets at these locations. Compared to recent reports of plastic ingestion by albatrosses off the east coast of South America, we confirm that plastic is seldom found in the stomachs of Thalassarche albatrosses off South Africa, but found no Diomedea albatrosses to contain plastic, compared to 26% off South America. The reasons for such regional differences are unclear, but emphasize the importance of reporting negative as well as positive records of plastic ingestion by marine biota. Copyright © 2016 Elsevier Ltd. All rights reserved.

Growth inhibition of Saccharomyces cerevisiae by the immunosuppressant leflunomide is due to the inhibition of uracil uptake via Fur4p.

PubMed

Fujimura, H

1998-10-01

The immunosuppressant leflunomide inhibits cytokine-stimulated proliferation of lymphoid cells in vitro and also inhibits the growth of the eukaryotic microorganism Saccharomyces cerevisiae. To elucidate the molecular mechanism of action of the drug, two yeast genes which suppress the anti-proliferative effect when present in multiple copies were cloned and designated MLF1 and MLF2 for multicopy suppressor of leflunomide sensitivity. DNA sequencing analysis revealed that the MLF1 gene is identical to the FUR4 gene, which encodes a uracil permease and functions to import uracil efficiently. The MLF2 was found to be identical to the URA3 gene. Excess exogenous uracil also overcomes the anti-proliferative effect of leflunomide on yeast cells. Uracil prototrophy also conferred resistance to leflunomide. Uracil uptake was inhibited by leflunomide. Thus, the growth inhibition by leflunomide seen in a S. cerevisiae ura3 auxotroph is due to the inhibition of the entry of exogenous uracil via the Fur4 uracil permease.

Deeply torpid bats can change position without elevation of body temperature.

PubMed

Bartonička, Tomáš; Bandouchova, Hana; Berková, Hana; Blažek, Ján; Lučan, Radek; Horáček, Ivan; Martínková, Natália; Pikula, Jiri; Řehák, Zdeněk; Zukal, Jan

2017-01-01

Because body temperature is tightly coupled to physiological function, hibernating animals entering deep torpor are typically immobile. We analysed thermal behaviour and locomotory activity of hibernating greater mouse-eared bats Myotis myotis and found two types of movement behaviour related to body temperature, i.e. movement at high fur temperature and at low fur temperatures (Tflow; <5°C). First Tflow movements appeared at the beginning of March and often occurred during long torpor bouts. In most cases, Tflow events represented slow displacements between clusters of bats. In several cases, however, departure or arrivals from and into clusters was also recorded without any elevation in body temperature. Distance travelled, flight duration and speed of locomotion during Tflow events was lower than in high fur temperature events. Such behaviour could allow bats to save energy long-term and prolong torpor bouts. Tflow movement in torpid bats significantly changes our understanding of basic hibernation principles and we strongly recommend further studies on the subject. Copyright © 2016. Published by Elsevier Ltd.

Skin and fur bacterial diversity and community structure on American southwestern bats: effects of habitat, geography and bat traits

PubMed Central

Hathaway, Jennifer J.M.; Kimble, Jason C.; Buecher, Debbie C.; Valdez, Ernest W.; Young, Jesse M.; Read, Kaitlyn J.H.; Northup, Diana E.

2017-01-01

Microorganisms that reside on and in mammals, such as bats, have the potential to influence their host’s health and to provide defenses against invading pathogens. However, we have little understanding of the skin and fur bacterial microbiota on bats, or factors that influence the structure of these communities. The southwestern United States offers excellent sites for the study of external bat bacterial microbiota due to the diversity of bat species, the variety of abiotic and biotic factors that may govern bat bacterial microbiota communities, and the lack of the newly emergent fungal disease in bats, white-nose syndrome (WNS), in the southwest. To test these variables, we used 16S rRNA gene 454 pyrosequencing from swabs of external skin and fur surfaces from 163 bats from 13 species sampled from southeastern New Mexico to northwestern Arizona. Community similarity patterns, random forest models, and generalized linear mixed-effects models show that factors such as location (e.g., cave-caught versus surface-netted) and ecoregion are major contributors to the structure of bacterial communities on bats. Bats caught in caves had a distinct microbial community compared to those that were netted on the surface. Our results provide a first insight into the distribution of skin and fur bat bacteria in the WNS-free environment of New Mexico and Arizona. More importantly, it provides a baseline of bat external microbiota that can be explored for potential natural defenses against pathogens. PMID:29093998

Skin and fur bacterial diversity and community structure on American southwestern bats: effects of habitat, geography and bat traits.

PubMed

Winter, Ara S; Hathaway, Jennifer J M; Kimble, Jason C; Buecher, Debbie C; Valdez, Ernest W; Porras-Alfaro, Andrea; Young, Jesse M; Read, Kaitlyn J H; Northup, Diana E

2017-01-01

Microorganisms that reside on and in mammals, such as bats, have the potential to influence their host's health and to provide defenses against invading pathogens. However, we have little understanding of the skin and fur bacterial microbiota on bats, or factors that influence the structure of these communities. The southwestern United States offers excellent sites for the study of external bat bacterial microbiota due to the diversity of bat species, the variety of abiotic and biotic factors that may govern bat bacterial microbiota communities, and the lack of the newly emergent fungal disease in bats, white-nose syndrome (WNS), in the southwest. To test these variables, we used 16S rRNA gene 454 pyrosequencing from swabs of external skin and fur surfaces from 163 bats from 13 species sampled from southeastern New Mexico to northwestern Arizona. Community similarity patterns, random forest models, and generalized linear mixed-effects models show that factors such as location (e.g., cave-caught versus surface-netted) and ecoregion are major contributors to the structure of bacterial communities on bats. Bats caught in caves had a distinct microbial community compared to those that were netted on the surface. Our results provide a first insight into the distribution of skin and fur bat bacteria in the WNS-free environment of New Mexico and Arizona. More importantly, it provides a baseline of bat external microbiota that can be explored for potential natural defenses against pathogens.

Pelage insulation, litter size, and ambient temperature impact maternal energy intake and offspring development during lactation

PubMed Central

Paul, Matthew J.; Tuthill, Christiana; Kauffman, Alexander S.; Zucker, Irving

2010-01-01

Energy balance during lactation critically influences survival and growth of a mother’s offspring, and hence, her reproductive success. Most experiments have investigated the influence of a single factor (e.g., ambient temperature [Ta] or litter size) on the energetics of lactation. Here, we determined the impact of multiple interventions, including increased conductive heat loss consequent to dorsal fur removal, cold exposure (Ta of 5°C versus 23°C), and differential lactational load from litters of different sizes (2 or 4 pups), on maternal energy balance and offspring development of Siberian hamsters (Phodopus sungorus). Lower Ta, fur removal, and larger litters were associated with increased maternal food consumption. Females exposed to multiple challenges (e.g., both fur loss and lower Ta) ate substantially more food than those exposed to a single challenge, with no apparent ceiling to elevated food intake (increases up to 538%). Thus, energy intake of dams under these conditions does not appear to be limited by feeding behavior or the size of the digestive tract. Housing at 5°C attenuated pup weight gain and increased pup mortality to more than 5 times that of litters housed at 23°C. Increases in the dam’s conductive heat loss induced by fur removal did not affect pup weight gain or survival, suggesting that effects of low Ta on pup weight gain and survival reflect limitations in the pups’ ability to ingest or incorporate energy. PMID:20184907

Skin and fur bacterial diversity and community structure on American southwestern bats: effects of habitat, geography and bat traits

USGS Publications Warehouse

Winter, Ara S.; Hathaway, Jennifer J. M.; Kimble, Jason C.; Buecher, Debbie C.; Valdez, Ernest W.; Porras-Alfaro, Andrea; Young, Jesse M.; Read, Kaitlyn J. H.; Northup, Diana E.

2017-01-01

Microorganisms that reside on and in mammals, such as bats, have the potential to influence their host’s health and to provide defenses against invading pathogens. However, we have little understanding of the skin and fur bacterial microbiota on bats, or factors that influence the structure of these communities. The southwestern United States offers excellent sites for the study of external bat bacterial microbiota due to the diversity of bat species, the variety of abiotic and biotic factors that may govern bat bacterial microbiota communities, and the lack of the newly emergent fungal disease in bats, white-nose syndrome (WNS), in the southwest. To test these variables, we used 16S rRNA gene 454 pyrosequencing from swabs of external skin and fur surfaces from 163 bats from 13 species sampled from southeastern New Mexico to northwestern Arizona. Community similarity patterns, random forest models, and generalized linear mixed-effects models show that factors such as location (e.g., cave-caught versus surface-netted) and ecoregion are major contributors to the structure of bacterial communities on bats. Bats caught in caves had a distinct microbial community compared to those that were netted on the surface. Our results provide a first insight into the distribution of skin and fur bat bacteria in the WNS-free environment of New Mexico and Arizona. More importantly, it provides a baseline of bat external microbiota that can be explored for potential natural defenses against pathogens.

Prevalence and zoonotic risks of Trichophyton mentagrophytes and Cheyletiella spp. in guinea pigs and rabbits in Dutch pet shops.

PubMed

Overgaauw, P A M; Avermaete, K H A van; Mertens, C A R M; Meijer, M; Schoemaker, N J

2017-06-01

Young rabbits and guinea pigs are often purchased as pets for children and may be infected with zoonotic skin infections. To assess the risk of acquiring such an infection from rabbits or guinea pigs, this study investigated the prevalence of the fungus Trichophyton mentagrophytes and the fur mite Cheyletiella parasitovorax in asymptomatic rabbits and guinea pigs in Dutch pet shops. In 91 pet shops a total of 213 rabbits and 179 guinea pigs were sampled using the Mackenzie technique and cultured. Clean cultures were examined microscopically and a PCR was performed on at least one sample from each pet shop. All animals were investigated for fur mite using a flea comb, a magnifying glass and white paper. From the fur of 3.8% (8/213) of the rabbits and 16.8% (30/179) of the guinea pigs, T. mentagrophytes was isolated. From 1 guinea pig (0,6%) Chrysosporium keratinophilum was isolated. Dermatophyte-positive rabbits and guinea pigs originated from 5.6% (5/90) and 27.3% (24/88) of the investigated pet shops, respectively. Fur mites were not found. Pet shops can play an important role in preventing transmission of zoonotic ringworm infections (dermatophytosis) and educating their customers. Specific preventive measures such as routine screening examinations and (prophylactic) treatment of rabbits and guinea pigs are recommended next to regular hygiene when handling animals. Copyright © 2017 Elsevier B.V. All rights reserved.

Comparison of shockwave lithotripsy and flexible ureteroscopy for the treatment of kidney stones in patients with a solitary kidney.

PubMed

Yuruk, Emrah; Binbay, Murat; Ozgor, Faruk; Sekerel, Levent; Berberoglu, Yalcin; Muslumanoglu, Ahmet Yaser

2015-04-01

To compare the outcomes of these minimally invasive procedures in this patient population. The database of our institution has been retrospectively reviewed, and medical records of urolithiasis patients with a solitary kidney who underwent flexible ureteroscopy (F-URS) or extracorporeal shock wave lithotripsy (SWL) between January 2009 and December 2012 were examined. Retreatment rates, complications, changes in estimated glomerular filtration rates (eGFRs), chronic kidney disease (CKD) stages, and stone-free rates were compared between the two groups. Stones of 48 patients (mean age: 48.8±15.4, range: 14-76) with solitary kidneys were treated with SWL (n=30, 62.5%) or F-URS (n=18, 37.5%). Patient demographics and stone related parameters were similar. The most common stone location was the pelvis in the SWL group (36.6%), whereas it was the pelvis and a calix in the F-URS group (38.8%). Complications and success rates were similar in both groups, however, patients in the SWL group needed more sessions to achieve stone clearance (2.2±0.89 vs 1.06±0.24, p=0.0001). Preoperative and postoperative eGFR and CKD stage changes were also similar. Both SWL and F-URS are effective and safe techniques, which can be used for the treatment of stones in patients with solitary kidneys. However, patients treated with SWL need more sessions to achieve stone clearance.

Hydrogen-peroxide-induced oxidative stress responses in Desulfovibrio vulgaris Hildenborough

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhou, A.; He, Z.; Redding-Johanson, A.M.

2010-07-01

To understand how sulphate-reducing bacteria respond to oxidative stresses, the responses of Desulfovibrio vulgaris Hildenborough to H{sub 2}O{sub 2}-induced stresses were investigated with transcriptomic, proteomic and genetic approaches. H{sub 2}O{sub 2} and induced chemical species (e.g. polysulfide, ROS) and redox potential shift increased the expressions of the genes involved in detoxification, thioredoxin-dependent reduction system, protein and DNA repair, and decreased those involved in sulfate reduction, lactate oxidation and protein synthesis. A gene coexpression network analysis revealed complicated network interactions among differentially expressed genes, and suggested possible importance of several hypothetical genes in H{sub 2}O{sub 2} stress. Also, most of themore » genes in PerR and Fur regulons were highly induced, and the abundance of a Fur regulon protein increased. Mutant analysis suggested that PerR and Fur are functionally overlapped in response to stresses induced by H{sub 2}O{sub 2} and reaction products, and the upregulation of thioredoxin-dependent reduction genes was independent of PerR or Fur. It appears that induction of those stress response genes could contribute to the increased resistance of deletion mutants to H{sub 2}O{sub 2}-induced stresses. In addition, a conceptual cellular model of D. vulgaris responses to H{sub 2}O{sub 2} stress was constructed to illustrate that this bacterium may employ a complicated molecular mechanism to defend against the H{sub 2}O{sub 2}-induced stresses.« less

Biochemical characterization of ethanol-dependent reduction of furfural by alcohol dehydrogenases.

PubMed

Li, Qunrui; Metthew Lam, L K; Xun, Luying

2011-11-01

Lignocellulosic biomass is usually converted to hydrolysates, which consist of sugars and sugar derivatives, such as furfural. Before yeast ferments sugars to ethanol, it reduces toxic furfural to non-inhibitory furfuryl alcohol in a prolonged lag phase. Bioreduction of furfural may shorten the lag phase. Cupriavidus necator JMP134 rapidly reduces furfural with a Zn-dependent alcohol dehydrogenase (FurX) at the expense of ethanol (Li et al. 2011). The mechanism of the ethanol-dependent reduction of furfural by FurX and three homologous alcohol dehydrogenases was investigated. The reduction consisted of two individual reactions: ethanol-dependent reduction of NAD(+) to NADH and then NADH-dependent reduction of furfural to furfuryl alcohol. The kinetic parameters of the coupled reaction and the individual reactions were determined for the four enzymes. The data indicated that limited NADH was released in the coupled reaction. The enzymes had high affinities for NADH (e.g., K ( d ) of 0.043 μM for the FurX-NADH complex) and relatively low affinities for NAD(+) (e.g., K ( d ) of 87 μM for FurX-NAD(+)). The kinetic data suggest that the four enzymes are efficient "furfural reductases" with either ethanol or NADH as the reducing power. The standard free energy change (ΔG°') for ethanol-dependent reduction of furfural was determined to be -1.1 kJ mol(-1). The physiological benefit for ethanol-dependent reduction of furfural is likely to replace toxic and recalcitrant furfural with less toxic and more biodegradable acetaldehyde.

Marked dissociation between hypothalamic-pituitary-adrenal activation and long-term behavioral effects in rats exposed to immobilization or cat odor.

PubMed

Muñoz-Abellán, C; Andero, R; Nadal, R; Armario, A

2008-09-01

Exposure of rodents to cats or certain cat odors results in long-term behavioral effects reminiscent of enhanced anxiety that have been considered to model post-traumatic stress disorder. However, other severe stressors such as tail-shock or immobilization in wooden boards (IMO) appear to induce shorter lasting changes in anxiety. In addition, there are controversial results regarding the effects of urine/feces odors. In the present work, we studied in two experiments the relationship between the degree of stress experienced by the animals during exposure to IMO, urine odors or fur odors (as assessed by hypothalamic-pituitary-adrenal activation and plasma glucose) and the short- and long-term behavioral consequences. In the first experiment, rats were individually exposed for 15 min to a novel environment (white large cages) containing either clean cat litter (controls) or litter soiled by cats (urine odors). Half of the rats in each condition were left to freely explore the environment whereas the others were subjected to immobilization (IMO) within the cages. Although ACTH, corticosterone and glucose responses to IMO were much stronger than those to the white cages with clean litter or urine odors (which did not differ from each other), no effect of treatments on anxiety-like behavior in the elevated plus-maze (EPM) were found one week later. However, previous IMO exposure did cause sensitization of the ACTH response to the EPM. In the second experiment, the response to white large cages containing either no odor (controls), litter soiled by cats (urine odor) or a cloth impregnated with cat odor (fur odor) was compared. Urine and fur odors elicited similar ACTH and corticosterone responses that were higher than those of controls, but plasma glucose levels were slightly higher in rats exposed to fur odor. When compared to controls, activity was only diminished in the novel cages containing fur odor. Similarly, fur odor-exposed rats, but not those exposed to urine odor, showed signs of enhanced anxiety in the EPM seven days later, although the ACTH response to the EPM was similar in the three groups. The present data demonstrate: (a) a marked dissociation between the degree of ACTH, corticosterone and glucose responses to stressors and their long-term anxiety-like effects; (b) that the type of cat odor is critical in determining the short-term and long-term physiological and behavioral consequences of exposure; and (c) that plasma ACTH released during brief exposure to the EPM does not appear to reflect anxiety-like behavior.

Aeromagnetic and Gravity Surveys in Afghanistan: A Web Site for Distribution of Data

USGS Publications Warehouse

Sweeney, Ronald E.; Kucks, Robert P.; Hill, Patricia L.; Finn, Carol A.

2006-01-01

Aeromagnetic data were digitized from aeromagnetic maps created from aeromagnetic surveys flown in southeastern and southern Afghanistan in 1966 by PRAKLA, Gesellschaft fur praktische Lagerstattenforschung GmbH, Hannover, Germany, on behalf of the 'Bundesanstalt fur Bodenforschung', Hannover, Germany. The digitization was done along contour lines, followed by interpolation of the data along the original survey flight-lines. Survey and map specifications can be found in two project reports, 'prakla_report_1967.pdf' and 'bgr_report_1968.pdf', made available in this open-file report.

[The parallelisms in of sound signal of domestic sheep and Northern fur seals].

PubMed

Nikol'skiĭ, A A; Lisitsina, T Iu

2011-01-01

The parallelisms in communicative behavior of domestic sheep and Northern fur seals within a herd are accompanied by parallelisms in parameters of sound signal, the calling scream. This signal ensures ties between babies and their mothers at a long distance. The basis of parallelisms is formed by amplitude modulation at two levels: the one being a direct amplitude modulation of the carrier frequency and the other--modulation of the carrier frequency oscillation. Parallelisms in the signal oscillatory process result in corresponding parallelisms in the structure of its frequency spectrum.

Army Personnel Complied with the Berry Amendment but Can Improve Compliance with the Buy American Act

DTIC Science & Technology

2014-11-07

tools; • FSG 52 – measuring tools; • FSG 83 – textiles, leather, furs, apparel , and shoes; • FSG 84 – clothing , individual equipment and insignia; and...include: FSG 51 (Hand tools), 52 (Measuring tools), 83 (Textiles, leather, furs, apparel , and shoes), 84 ( Clothing , individual equipment and insignia...No. DODIG-2015-026 N O V E M B E R 7 , 2 0 1 4 Army Personnel Complied With the Berry Amendment But Can Improve Compliance With the Buy American

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with multidimensional scaling, binary hierarchical cluster tree and selected diagnostic masses improves species identification of Neolithic keratin sequences from furs of the Tyrolean Iceman Oetzi.

PubMed

Hollemeyer, Klaus; Altmeyer, Wolfgang; Heinzle, Elmar; Pitra, Christian

2012-08-30

The identification of fur origins from the 5300-year-old Tyrolean Iceman's accoutrement is not yet complete, although definite identification is essential for the socio-cultural context of his epoch. Neither have all potential samples been identified so far, nor there has a consensus been reached on the species identified using the classical methods. Archaeological hair often lacks analyzable hair scale patterns in microscopic analyses and polymer chain reaction (PCR)-based techniques are often inapplicable due to the lack of amplifiable ancient DNA. To overcome these drawbacks, a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method was used exclusively based on hair keratins. Thirteen fur specimens from his accoutrement were analyzed after tryptic digest of native hair. Peptide mass fingerprints (pmfs) from ancient samples and from reference species mostly occurring in the Alpine surroundings at his lifetime were compared to each other using multidimensional scaling and binary hierarchical cluster tree analysis. Both statistical methods highly reflect spectral similarities among pmfs as close zoological relationships. While multidimensional scaling was useful to discriminate specimens on the zoological order level, binary hierarchical cluster tree reached the family or subfamily level. Additionally, the presence and/or absence of order, family and/or species-specific diagnostic masses in their pmfs allowed the identification of mammals mostly down to single species level. Red deer was found in his shoe vamp, goat in the leggings, cattle in his shoe sole and at his quiver's closing flap as well as sheep and chamois in his coat. Canid species, like grey wolf, domestic dog or European red fox, were discovered in his leggings for the first time, but could not be differentiated to species level. This is widening the spectrum of processed fur-bearing species to at least one member of the Canidae family. His fur cap was allocated to a carnivore species, but differentiation between brown bear and a canid species could not be made with certainty. Copyright © 2012 John Wiley & Sons, Ltd.

Resuspension of biological particles from indoor surfaces: Effects of humidity and air swirl.

PubMed

Salimifard, Parichehr; Rim, Donghyun; Gomes, Carlos; Kremer, Paul; Freihaut, James D

2017-04-01

Human exposure to airborne particles can lead to adverse health outcomes such as respiratory and allergic symptoms. Understanding the transport mechanism of respirable particles in occupied spaces is a first step towards assessing inhalation exposure. Several studies have contributed to the current knowledge of particle resuspension from indoor surfaces; however, few published studies are available on resuspension of biological particles from indoor surfaces. The objective of this study is to investigate the impacts of humidity and air swirl on resuspension of biological particles from floor and duct surfaces. Controlled laboratory experiments were conducted under varying degrees of humidity and airflow conditions. Resuspension rates of five types of particles (quartz, dust mite, cat fur, dog fur, and bacterial spore-Bacillus thuringiensis as an anthrax simulant) were determined for two types of floor surface (carpet and linoleum) and a duct surface (galvanized sheet metal). The results show that the particle property of being hydrophilic or hydrophobic plays an important role in particle resuspension rate. Resuspension rates of hydrophilic dust mite particles increase up to two orders of magnitude as relative humidity (RH) decreased from 80% to 10% at 25°C. However, resuspension rates of cat fur and dog fur particles that are hydrophobic are within the measurement error range (±15%) over 10-80% RH. With regard to resuspension of bacterial spores (Bacillus thuringiensis) from a duct surface, the resuspension rates are substantially affected by air swirl velocity and particle size. However, no discernible increase in particle resuspension was observed with duct vibration. Copyright © 2017 Elsevier B.V. All rights reserved.

Cat odor causes long-lasting contextual fear conditioning and increased pituitary-adrenal activation, without modifying anxiety.

PubMed

Muñoz-Abellán, Cristina; Daviu, Nuria; Rabasa, Cristina; Nadal, Roser; Armario, Antonio

2009-10-01

A single exposure to a cat or cat odors has been reported by some groups to induce contextual and auditory fear conditioning and long-lasting changes in anxiety-like behaviour, but there is no evidence for parallel changes in biological stress markers. In the present study we demonstrated in male rats that exposure to a novel environment containing a cloth impregnated with cat fur odor resulted in avoidance of the odor, lower levels of activity and higher pituitary-adrenal (PA) response as compared to those exposed to the novel environment containing a clean cloth, suggesting increased levels of stress in the former animals. When re-exposed 9 days later to the same environment with a clean cloth, previously cat fur exposed rats again showed avoidance of the cloth area and lower levels of activity, suggesting development of contextual fear conditioning, which again was associated with a higher PA activation. In contrast, unaltered both anxiety-like behaviour and PA responsiveness to an elevated plus-maze were found 7 days after cat odor exposure. It is concluded that: (i) PA activation is able to reflect both the stressful properties of cat fur odor and odor-induced contextual fear conditioning; (ii) development of cat odor-induced contextual fear conditioning is independent of the induction of long-lasting changes in anxiety-like behaviour; and (iii) greater PA activation during exposure to the odor context is not explained by non-specific sensitization of the PA axis caused by previous exposure to cat fur odor.

Deletion of the Uracil Permease Gene Confers Cross-Resistance to 5-Fluorouracil and Azoles in Candida lusitaniae and Highlights Antagonistic Interaction between Fluorinated Nucleotides and Fluconazole

PubMed Central

Gabriel, Frédéric; Sabra, Ayman; El-Kirat-Chatel, Sofiane; Pujol, Sophie; Fitton-Ouhabi, Valérie; Brèthes, Daniel; Dementhon, Karine; Accoceberry, Isabelle

2014-01-01

We characterized two additional membrane transporters (Fur4p and Dal4p) of the nucleobase cation symporter 1 (NCS1) family involved in the uptake transport of pyrimidines and related molecules in the opportunistic pathogenic yeast Candida lusitaniae. Simple and multiple null mutants were constructed by gene deletion and genetic crosses. The function of each transporter was characterized by supplementation experiments, and the kinetic parameters of the uptake transport of uracil were measured using radiolabeled substrate. Fur4p specifically transports uracil and 5-fluorouracil. Dal4p is very close to Fur4p and transports allantoin (glyoxyldiureide). Deletion of the FUR4 gene confers resistance to 5-fluorouracil as well as cross-resistance to triazoles and imidazole antifungals when they are used simultaneously with 5-fluorouracil. However, the nucleobase transporters are not involved in azole uptake. Only fluorinated pyrimidines, not pyrimidines themselves, are able to promote cross-resistance to azoles by both the salvage and the de novo pathway of pyrimidine synthesis. A reinterpretation of the data previously obtained led us to show that subinhibitory doses of 5-fluorocytosine, 5-fluorouracil, and 5-fluorouridine also were able to trigger resistance to fluconazole in susceptible wild-type strains of C. lusitaniae and of different Candida species. Our results suggest that intracellular fluorinated nucleotides play a key role in azole resistance, either by preventing azoles from targeting the lanosterol 14-alpha-demethylase or its catalytic site or by acting as a molecular switch for the triggering of efflux transport. PMID:24867971

Binding of the Zn2+ ion to ferric uptake regulation protein from E. coli and the competition with Fe2+ binding: a molecular modeling study of the effect on DNA binding and conformational changes of Fur

NASA Astrophysics Data System (ADS)

Jabour, Salih; Hamed, Mazen Y.

2009-04-01

The three dimensional structure of Ferric uptake regulation protein dimer from E. coli, determined by molecular modeling, was docked on a DNA fragment (iron box) and Zn2+ ions were added in two steps. The first step involved the binding of one Zn2+ ion to what is known as the zinc site which consists of the residues Cys 92, Cys 95, Asp 137, Asp141, Arg139, Glu 140, His 145 and His 143 with an average metal-Nitrogen distance of 2.5 Å and metal-oxygen distance of 3.1-3.2 Å. The second Zn2+ ion is bound to the iron activating site formed from the residues Ile 50, His 71, Asn 72, Gly 97, Asp 105 and Ala 109. The binding of the second Zn2+ ion strengthened the binding of the first ion as indicated by the shortening of the zinc-residue distances. Fe2+, when added to the complex consisting of 2Zn2+/Fur dimer/DNA, replaced the Zn2+ ion in the zinc site and when a second Fe2+ was added, it replaced the second zinc ion in the iron activating site. The binding of both zinc and iron ions induced a similar change in Fur conformations, but shifted residues closer to DNA in a different manner. This is discussed along with a possible role for the Zn2+ ion in the Fur dimer binding of DNA in its repressor activity.

The Iron-Responsive Fur/RyhB Regulatory Cascade Modulates the Shigella Outer Membrane Protease IcsP ▿ †

PubMed Central

Africa, Lia A. A.; Murphy, Erin R.; Egan, Nicholas R.; Wigley, Amanda F.; Wing, Helen J.

2011-01-01

Actin-based motility is central to the pathogenicity of the intracellular bacterial pathogen Shigella. Two Shigella outer membrane proteins, IcsA and IcsP, are required for efficient actin-based motility in the host cell cytoplasm, and the genes encoding both proteins are carried on the large virulence plasmid. IcsA triggers actin polymerization on the surface of the bacterium, leading to the formation of an actin tail that allows both intra- and intercellular spread. IcsP, an outer membrane protease, modulates the amount and distribution of the IcsA protein on the bacterial surface through proteolytic cleavage of IcsA. Transcription of icsP is increased in the presence of VirB, a DNA-binding protein that positively regulates many genes carried on the large virulence plasmid. In Shigella dysenteriae, the small regulatory RNA RyhB, which is a member of the iron-responsive Fur regulon, suppresses several virulence-associated phenotypes by downregulating levels of virB in response to iron limitation. Here we show that the Fur/RyhB regulatory pathway downregulates IcsP levels in response to low iron concentrations in Shigella flexneri and that this occurs at the level of transcription through the RyhB-dependent regulation of VirB. These observations demonstrate that in Shigella species the Fur/RyhB regulatory pathway provides a mechanism to finely tune the expression of icsP in response to the low concentrations of free iron predicted to be encountered within colonic epithelial cells. PMID:21859852

Benzo(a)pyrene and X-rays induce reversions of the pink-eyed unstable mutation in the retinal pigment epithelium of mice.

PubMed

Bishop, A J; Kosaras, B; Sidman, R L; Schiestl, R H

2000-12-20

The pink-eyed unstable (p(un)) mutation is the result of a 70kb tandem duplication within the murine p gene. Homologous deletion/recombination of the locus to wild-type occurs spontaneously in embryos and results in pigmented spots in the fur and eye that persist for life. Such deletion events are also inducible by a variety of DNA damaging agents, as we have observed previously with the fur spot assay. Here, we describe the use of the retinal pigment epithelium (RPE) of the eye to detect reversion events induced with two differently acting agents. Benzo(a)pyrene (B(a)P) induces a high frequency, and X-ray exposure a more modest increase, of p(un) reversion in both the fur and the eye. The eye-spot assay requires fewer mice for significant results than the fur spot assay. Previous work had elucidated the cell proliferation pattern in the RPE and a position effect variegation phenotype in the pattern of p(un) reversions, which we have confirmed. Acute exposure to B(a)P or X-rays resulted in an increased frequency of reversion events. The majority of the spontaneous reversions lie toward the periphery of the RPE whereas induced events are found more centrally, closer to the optic nerve head. The induced distribution corresponds to the major sites of cell proliferation in the RPE at the time of exposure, and further advocates the proposal that dividing cells are at highest risk to develop deletions.

Effects of a 17q21 chromosome gene variant, tobacco smoke and furred pets on infant wheeze.

PubMed

Bräuner, E V; Loft, S; Raaschou-Nielsen, O; Vogel, U; Andersen, P S; Sørensen, M

2012-01-01

The first common genetic factor identified for pediatric asthma by genome-wide association is the chromosome 17q21 locus, harbouring the ORMDL3 gene. ORMDL3 is involved in facilitation of endoplasmic reticulum-mediated inflammatory responses, believed to underlie its asthma association. We investigated associations between the rs7216389 polymorphism in the 17q21 locus affecting ORMDL3 expression and the risk for recurrent wheeze and interactions with exposure to tobacco smoke and furred pets during pregnancy and infancy using a birth cohort of 101,042 infants. Rs7216389 was significantly associated with recurrent wheeze risk among 18-month-old infants. There was a 1.35-fold higher risk of recurrent wheeze among homozygous variant allele carriers compared with homozygous wild-type allele carriers. There was significant interaction between rs7216389 and domestic furred pets, with a positive association between pets and wheeze among homozygous wild-type carriers and a negative association among homozygous variant allele carriers. There was no interaction between rs7216389 and tobacco smoke exposure.

Lunar cycles in diel prey migrations exert a stronger effect on the diving of juveniles than adult Galápagos fur seals.

PubMed Central

Horning, M; Trillmich, F

1999-01-01

In our study of the development of diving in Galápagos fur seals, we analysed changes in diving activity and body mass trends over the lunar cycle. Based on previously observed lunar cycles in colony attendance patterns, we hypothesized a greater impact of prey migrations of deep scattering layer organisms on younger fur seals. Using electronic dive recorders, we determined that seals dived less and deeper on moonlit nights than at new moon, and incurred body mass losses. These changes in foraging over the lunar cycle correlate with the suppression of the vertical migration of prey by lunar light. All effects were more pronounced in juveniles than adult females, with greater relative mass loss during full moon, which must (i) negatively affect long-term juvenile growth rates, (ii) lengthen periods of maternal dependence, and (iii) contribute to the lowest reproductive rate reported for seals. This underlines the importance of studying ontogeny in order to understand life histories, and for determining the susceptibility of animal populations to fluctuations in food availability. PMID:10406130

Leptin and leptin receptor gene polymorphisms are correlated with production performance in the Arctic fox.

PubMed

Zhang, M; Bai, X J

2015-05-25

The polymerase chain reaction-single-strand conformation polymorphism technique was employed to measure mononucleotide diversity in the coding region of the leptin and leptin receptor genes in the Arctic fox. The relationships between specific genetic mutations and reproductive performance in Arctic foxes were determined to im-prove breeding strategies. We found that a leptin gene polymorphism was significantly associated with body weight (P < 0.01), abdominal circumference (P < 0.01), and fur length (P < 0.01). Furthermore, a polymorphism in the leptin receptor gene was associated with carcass weight and guard hair length (P < 0.01). Leptin and leptin receptor gene combinatorial genotypes were significantly associated with abdominal circumference, fur length (P < 0.01), and body weight (P < 0.05). The leptin gene is thus a key gene affecting body weight, abdominal circumference, and fur length in Arctic foxes, whereas variations in the leptin receptor mainly affect carcass weight and guard hair. The marker loci identified in this study can be used to assist in the selection of Arctic foxes for breeding to raise the production performance of this species.

Keeping warm with fur in cold water: entrainment of air in hairy surfaces

NASA Astrophysics Data System (ADS)

Nasto, Alice; Regli, Marianne; Brun, Pierre-Thomas; Clanet, Christophe; Hosoi, Anette

2015-11-01

Instead of relying on a thick layer of body fat for insulation as many aquatic mammals do, fur seals and otters trap air in their dense fur for insulation in cold water. Using a combination of model experiments and theory, we rationalize this mechanism of air trapping underwater for thermoregulation. For the model experiments, hairy surfaces are fabricated using laser cut molds and casting samples with PDMS. Modeling the hairy texture as a network of capillary tubes, the imbibition speed of water into the hairs is obtained through a balance of hydrostatic pressure and viscous stress. In this scenario, the bending of the hairs and capillary forces are negligible. The maximum diving depth that can be achieved before the hairs are wetted to the roots is predicted from a comparison of the diving speed and imbibition speed. The amount of air that is entrained in hairy surfaces is greater than what is expected for classic Landau-Levich-Derjaguin plate plunging. A phase diagram with the parameters from experiments and biological data allows a comparison of the model system and animals.

Radiative contribution to thermal conductance in animal furs and other woolly insulators.

PubMed

Simonis, Priscilla; Rattal, Mourad; Oualim, El Mostafa; Mouhse, Azeddine; Vigneron, Jean-Pol

2014-01-27

This paper deals with radiation's contribution to thermal insulation. The mechanism by which a stack of absorbers limits radiative heat transfer is examined in detail both for black-body shields and grey-body shields. It shows that radiation energy transfer rates should be much faster than conduction rates. It demonstrates that, for opaque screens, increased reflectivity will dramatically reduce the rate of heat transfer, improving thermal insulation. This simple model is thought to contribute to the understanding of how animal furs, human clothes, rockwool insulators, thermo-protective containers, and many other passive energy-saving devices operate.

Wet-dog shake

NASA Astrophysics Data System (ADS)

Dickerson, Andrew; Mills, Zack; Hu, David

2010-11-01

The drying of wet fur is a critical to mammalian heat regulation. We investigate experimentally the ability of hirsute animals to rapidly oscillate their bodies to shed water droplets, nature's analogy to the spin cycle of a washing machine. High-speed videography and fur-particle tracking is employed to determine the angular position of the animal's shoulder skin as a function of time. We determine conditions for drop ejection by considering the balance of surface tension and centripetal forces on drops adhering to the animal. Particular attention is paid to rationalizing the relationship between animal size and oscillation frequency required to self-dry.

Large volcanic eruptions and the PETM: Geochemistry from the Fur Formation, Denmark

NASA Astrophysics Data System (ADS)

Jones, Morgan; Svensen, Henrik; Tegner, Christian; Planke, Sverre; Willumsen, Pi

2015-04-01

The opening of the North Atlantic Ocean during the early Cenozoic was accompanied by substantial volcanism that resulted in the deposition of numerous ash layers over much of northern Europe. This volcanism was contemporaneous with the extreme greenhouse climate of the Palaeocene-Eocene Thermal Maximum (PETM), and is therefore of particular interest for volcanism-climate interactions. The island of Fur, northern Denmark, contains abundant outcrops of volcanic ash layers deposited in a shallow marine environment. Over 179 distinct ash horizons (those greater than ~1 cm are numbered #-39 to #+140) are found within the ~60 m thick Fur Formation. The ash layers are predominantly black and composed of volcanic glass particles ranging from silt to sand in size. Each bed is normally graded and lacks any evidence of significant reworking. There were no volcanoes in the vicinity of Fur during this time, and the outcrops are >700 km from the break-up axis, indicating that at least some of the ash layers were formed during very large eruptions and transported a long way from the source volcanoes. A few thick grey ash layers (e.g. #-33 and #+19) are believed to have originated from volcanoes in East Greenland. Here we present geochemical data from two key sections within the Fur Formation, a beach section at Stolleklint where ashes #-34 to #-31 are exposed, and a quarry section at Jenshøj that covers ashes #+17 to #+35. The #-33 and #+19 ashes are both prominent marker horizons around 15-20 cm thick. The Stolleklint section is clay rich while the quarry section is dominated by diatomite. At Stolleklint, bulk rock total organic carbon (TOC) δ13C values throughout a 1.5 m section are relatively steady at -30.7 to -31.7 o typical of the carbon isotope excursion values of the PETM at Fur. Just above ash layer #-33, δ13C = -27.9 o characteristic of post-PETM values. The bulk rock TOC is high, 1.5 to 4 wt. %. The post-PETM quarry section is much poorer in organic material (0-0.5 wt. % TOC). Values of δ13C are more varied than at Stolleklint, scatter around -28.0 o in the lower 0.5 m. A small negative δ13C excursion occurs just above the thick #+19 ash layer, followed by a slow recovery to less negative values up section. This suggests a possible causal relation between the ash deposition and the carbon isotope record, as diminished primary productivity is typified by negative δ13C TOC values. These results indicate that North-Atlantic volcanism had significant repercussions and could have played a role in the termination of the PETM.

Flexible ureterorenoscopy is safe and efficient for the treatment of kidney stones in patients with chronic kidney disease.

PubMed

Yuruk, Emrah; Binbay, Murat; Ozgor, Faruk; Erbin, Akif; Berberoglu, Yalcin; Muslumanoglu, Ahmet Y

2014-12-01

To evaluate the outcomes of kidney stone treatment using flexible ureterorenoscopy (f-URS) among patients with chronic kidney disease (CKD). Data of patients who underwent f-URS between January 2009 and December 2012 were collected. Patients were staged according to estimated glomerular filtration rate. Patients with stage ≥ 3 were accepted as having CKD (study group). These patients were matched with a group of patients without CKD (control group). Operative characteristics, complication rates, and third-month success rates were compared. Overall, 339 patients underwent f-URS and 62 (18.28%) had CKD. Control group constituted of 87 patients. Having a solitary kidney (17.4% vs 3.5%; P = .003) and history of stone intervention (51.6% vs 23%; P = .001) were more common in the CKD group. Similarly, access sheath was more commonly used among patients with CKD (87.1% vs 70.22%; P = .015). Both perioperative (19.35% vs 19.54; P = .372) and postoperative (22.6% vs 16.1%; P = .214) complication rates were similar in patients with and without CKD. Hospitalization time was 25.70 ± 25.62 and 24.5 ± 25 hours (P = .871) for patients with and without CKD, respectively. Although mean third postoperative estimated glomerular filtration rate of patients with CKD did not change significantly (48.16 ± 8.72 vs 49.08 ± 9.26; P = .431), CKD stage of 13 patients shifted from 3 to 2. At the third postoperative month, stone free rate in patients with and without CKD was 87.1% vs 86.2% (P = .875). f-URS is a safe and effective procedure in patients with CKD and it is associated with improved overall kidney function. Copyright © 2014 Elsevier Inc. All rights reserved.

Is There A Difference Between Presence of Single Stone And Multiple Stones in Flexible Ureterorenoscopy And Laser Lithotripsy For Renal Stone Burden < 300mm2 ?

PubMed

Ozgor, Faruk; Kucuktopcu, Onur; Ucpinar, Burak; Gurbuz, Zafer Gokhan; Sarilar, Omer; Berberoglu, Ahmet Yalcin; Baykal, Murat; Binbay, Murat

2016-01-01

In this study, we aim to evaluate and compare the effectiveness of flexible ureterorenoscopy (f-URS) for solitary and multiple renal stones with < 300 mm2 stone burden. Patients' charts who treated with f-URS for kidney stone between January 2010 and June 2015 were reviewed, retrospectively. Patients with solitary kidney stones (n:111) were enrolled in group 1. We selected 111 patients with multiple kidney stones to serve as the control group and the patients were matched at a 1:1 ratio with respect to the patient's age, gender, body mass index and stone burden. Additionally, patients with multiple stones were divided into two groups according to the presence or abscence of lower pole stones. Stone free status was accepted as complete stone clearence and presence of residual fragments < 2 mm. According to the study design; age, stone burden, body mass index were comparable between groups. The mean operation time was longer in group 2 (p= 0.229). However, the mean fluoroscopy screening time in group 1 and in group 2 was 2.1±1.7 and 2.6±1.5 min, respectively and significantly longer in patients with multiple renal stones (P=0.043). The stone-free status was significantly higher in patients with solitary renal stones after a single session procedure (p=0.02). After third month follow up, overall success rate was 92.7% in Group 1 and 86.4% in Group 2. Our study revealed that F-URS achieved better stone free status in solitary renal stones < 300 mm2. However, outcomes of F-URS were acceptable in patients with multiple stones. Copyright® by the International Brazilian Journal of Urology.

Epizootiology of Brucella infection in Australian fur seals.

PubMed

Lynch, Michael; Duignan, Pádraig J; Taylor, Trevor; Nielsen, Ole; Kirkwood, Roger; Gibbens, John; Arnould, John P Y

2011-04-01

Novel members of the bacterial genus Brucella have recently emerged as pathogens of various marine mammal species and as potential zoonotic agents. We investigated the epizootiology of Brucella infection in Australian fur seals (Arctocephalus pusillus doriferus) by establishing demographic and temporal variations in antibody prevalence, attempting isolation of the causative agent, and determining whether this potential pathogen is involved in frequent abortions observed in this pinniped species. Two competitive enzyme-linked immunosorbent assays (cELISAs), an indirect ELISA, and a fluorescence polarization assay (FPA) were used to test sera for Brucella antibodies. The FPA and cELISA proved suitable for use in this species. Significant differences in antibody prevalence were found between age classes of seals sampled between 2007 and 2009 at one colony. Pups sampled at this site (n=134) were negative for Brucella antibodies by all serologic tests but 17 of 45 (38%) of juveniles were antibody-positive. Antibody prevalence in adult females was significantly higher than in juveniles (P=0.044). Antibody prevalence for adult females between 2003 and 2009 varied significantly over time (P=0.011), and for individuals sampled between 2003 and 2005, the likelihood of pregnancy was greater in individuals positive for Brucella antibodies (P=0.034). Inflammatory lesions suggestive of infectious agents were found in 14 of 39 aborted Australian fur seal pups, but pathologic changes were not uniformly consistent for Brucella infection. Culture and PCR investigations on fetal tissues were negative for Brucella. Culture and PCR on selected fresh or frozen tissues from 36 juvenile and adult animals were also negative. We suspect that the prevalence of active infection with Brucella in Australian fur seals is low relative to antibody prevalence.

Deletion of the uracil permease gene confers cross-resistance to 5-fluorouracil and azoles in Candida lusitaniae and highlights antagonistic interaction between fluorinated nucleotides and fluconazole.

PubMed

Gabriel, Frédéric; Sabra, Ayman; El-Kirat-Chatel, Sofiane; Pujol, Sophie; Fitton-Ouhabi, Valérie; Brèthes, Daniel; Dementhon, Karine; Accoceberry, Isabelle; Noël, Thierry

2014-08-01

We characterized two additional membrane transporters (Fur4p and Dal4p) of the nucleobase cation symporter 1 (NCS1) family involved in the uptake transport of pyrimidines and related molecules in the opportunistic pathogenic yeast Candida lusitaniae. Simple and multiple null mutants were constructed by gene deletion and genetic crosses. The function of each transporter was characterized by supplementation experiments, and the kinetic parameters of the uptake transport of uracil were measured using radiolabeled substrate. Fur4p specifically transports uracil and 5-fluorouracil. Dal4p is very close to Fur4p and transports allantoin (glyoxyldiureide). Deletion of the FUR4 gene confers resistance to 5-fluorouracil as well as cross-resistance to triazoles and imidazole antifungals when they are used simultaneously with 5-fluorouracil. However, the nucleobase transporters are not involved in azole uptake. Only fluorinated pyrimidines, not pyrimidines themselves, are able to promote cross-resistance to azoles by both the salvage and the de novo pathway of pyrimidine synthesis. A reinterpretation of the data previously obtained led us to show that subinhibitory doses of 5-fluorocytosine, 5-fluorouracil, and 5-fluorouridine also were able to trigger resistance to fluconazole in susceptible wild-type strains of C. lusitaniae and of different Candida species. Our results suggest that intracellular fluorinated nucleotides play a key role in azole resistance, either by preventing azoles from targeting the lanosterol 14-alpha-demethylase or its catalytic site or by acting as a molecular switch for the triggering of efflux transport. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kamps, T; Barday, R; Jankowiak, A

In preparation for a high brightness, high average current electron source for the energy-recovery linac BERLinPro an all superconducting radio-frequency photoinjector is now in operation at Helmholtz-Zentrum Berlin. The aim of this experiment is beam demonstration with a high brightness electron source able to generate sub-ps pulse length electron bunches from a superconducting (SC) cathode film made of Pb coated on the backwall of a Nb SRF cavity. This paper describes the setup of the experiment and first results from beam measurements.

The bald and the beautiful: hairlessness in domestic dog breeds.

PubMed

Parker, Heidi G; Harris, Alexander; Dreger, Dayna L; Davis, Brian W; Ostrander, Elaine A

2017-02-05

An extraordinary amount of genomic variation is contained within the chromosomes of domestic dogs, manifesting as dramatic differences in morphology, behaviour and disease susceptibility. Morphology, in particular, has been a topic of enormous interest as biologists struggle to understand the small window of dog domestication from wolves, and the division of dogs into pure breeding, closed populations termed breeds. Many traits related to morphology, including body size, leg length and skull shape, have been under selection as part of the standard descriptions for the nearly 400 breeds recognized worldwide. Just as important, however, are the minor traits that have undergone selection by fanciers and breeders to define dogs of a particular appearance, such as tail length, ear position, back arch and variation in fur (pelage) growth patterns. In this paper, we both review and present new data for traits associated with pelage including fur length, curl, growth, shedding and even the presence or absence of fur. Finally, we report the discovery of a new gene associated with the absence of coat in the American Hairless Terrier breed.This article is part of the themed issue 'Evo-devo in the genomics era, and the origins of morphological diversity'. © 2016 The Authors.

Colorimetry provides a rapid objective measurement of de novo hair growth rate in mice.

PubMed

Tzung, Tien-Yi; Yang, Chia-Yi; Huang, Yung-Chang; Kao, Fu-Jen

2009-11-01

Depilated mice have been used as a test platform for hair growth-regulating agents. However, currently available assessment tools for hair growth in mice are less than ideal. Tristimulus colorimetry of the fur color of depilated agouti, albino, and black mice with L*, a*, and b* values were performed daily until the full growth of pelage. Using light-emitting diode (LED) irradiation (650 and 890 nm) with a daily dose of 3.5 J/cm(2) as hair growth regulators, the hair growth rates observed by the global assessment were compared with those derived from colorimetry. In contrast to a* and b* values, L* values changed more drastically over time in the anagen phase regardless of fur color. Unlike the inhibitory effect of 650 nm irradiation, LED of 890 nm promoted de novo hair regrowth in mice. The difference in hair growth rates detected by colorimetry paralleled the observation made by the global assessment. The L* value of fur color obtained by tristimulus colorimetry was a sensitive yet quantitative indicator of de novo hair growth, and could be used to project the hair growth rate in mice.

Flexible ureterorenoscopy in position or fusion anomaly: Is it feasible?

PubMed

Astolfi, Rafael Haddad; Freschi, Gustavo; Berti, Fernando Figueiredo; Gattas, Nelson; Molina, Wilson Rica; Meller, Alex

2017-08-01

To analyze the results of flexible ureterorenoscopy (F-URS) with holmium laser in the treatment of kidney stones with ectopic and fusion anomalies (horseshoe kidney and rotation anomalies). We reviewed data from 13 patients with fusion and ectopic renal anomalies that underwent F-URS from April 2011 to April 2017. We analyzed demographic and clinical data (age, gender, BMI, anatomical abnormality, location and dimension of the renal calculi) and perioperative data (method of treatment, stone-free rate, number of days with DJ catheter and perioperative complications). The mean stone size was 12.23 +/- 5.43 mm (range 6-22mm), located in the inferior (58.33%) and middle (16.76%) calyceal units, renal pelvis (16.67%) and multiple locations (8.33%). All 13 patients were treated with Ho-Yag laser, using dusting technique (25%), fragmentation and extraction of the calculi (58.33%) and mixed technique (16.67%). We did not have any severe perioperative complication. After 90 days, nine patients (75%) were considered stone free. Our data suggest that F-URS is a safe and feasible choice for the treatment of kidney stones in patients with renal ectopic and fusion anomalies.

Cladding Attachment Over Thick Exterior Insulating Sheathing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baker, P.; Eng, P.; Lepage, R.

The addition of insulation to the exterior of buildings is an effective means of increasing the thermal resistance of both wood framed walls as well as mass masonry wall assemblies. For thick layers of exterior insulation (levels greater than 1.5 inches), the use of wood furring strips attached through the insulation back to the structure has been used by many contractors and designers as a means to provide a convenient cladding attachment location (Straube and Smegal 2009, Pettit 2009, Joyce 2009, Ueno 2010). The research presented in this report is intended to help develop a better understanding of the systemmore » mechanics involved and the potential for environmental exposure induced movement between the furring strip and the framing. BSC sought to address the following research questions: 1.What are the relative roles of the mechanisms and the magnitudes of the force that influence the vertical displacement resistance of the system? 2.Can the capacity at a specified deflection be reliably calculated using mechanics based equations? 3.What are the impacts of environmental exposure on the vertical displacement of furring strips attached directly through insulation back to a wood structure?« less

Cladding Attachment Over Thick Exterior Insulating Sheathing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baker, P.; Eng, P.; Lepage, R.

The addition of insulation to the exterior of buildings is an effective means of increasing the thermal resistance of both wood framed walls as well as mass masonry wall assemblies. For thick layers of exterior insulation (levels greater than 1.5 inches), the use of wood furring strips attached through the insulation back to the structure has been used by many contractors and designers as a means to provide a convenient cladding attachment location (Straube and Smegal 2009, Pettit 2009, Joyce 2009, Ueno 2010). The research presented in this report is intended to help develop a better understanding of the systemmore » mechanics involved and the potential for environmental exposure induced movement between the furring strip and the framing. BSC sought to address the following research questions: 1. What are the relative roles of the mechanisms and the magnitudes of the force that influence the vertical displacement resistance of the system? 2. Can the capacity at a specified deflection be reliably calculated using mechanics based equations? 3. What are the impacts of environmental exposure on the vertical displacement of furring strips attached directly through insulation back to a wood structure?« less

The fur of mammals in exposed environments; do crypsis and thermal needs necessarily conflict? The polar bear and marsupial koala compared.

PubMed

Dawson, Terence J; Webster, Koa N; Maloney, Shane K

2014-02-01

The furs of mammals have varied and complex functions. Other than for thermoregulation, fur is involved in physical protection, sensory input, waterproofing and colouration, the latter being important for crypsis or camouflage. Some of these diverse functions potentially conflict. We have investigated how variation in cryptic colouration and thermal features may interact in the coats of mammals and influence potential heat inflows from solar radiation, much of which is outside the visible spectral range. The coats of the polar bear (Ursus maritimus) and the marsupial koala (Phascolarctus cinereus) have insulative similarities but, while they feature cryptic colouration, they are of contrasting colour, i.e. whitish and dark grey. The reflectance of solar radiation by coats was measured across the full solar spectrum using a spectroradiometer. The modulation of incident solar radiation and resultant heat flows in these coats were determined at a range of wind speeds by mounting them on a heat flux transducer/temperature-controlled plate apparatus in a wind tunnel. A lamp with a spectral distribution of radiation similar to the solar spectrum was used as a proxy for the sun. Crypsis by colour matching was apparent within the visible spectrum for the two species, U. maritimus being matched against snow and P. cinereus against Eucalyptus forest foliage. While reflectances across the full solar spectrum differed markedly, that of U. maritimus being 66 % as opposed to 10 % for P. cinereus, the heat influxes from solar radiation reaching the skin were similar. For both coats at low wind speed (1 m s(-1)), 19 % of incident solar radiation impacted as heat at the skin surface; at higher wind speed (10 m s(-1)) this decreased to approximately 10 %. Ursus maritimus and P. cinereus have high and comparable levels of fur insulation and although the patterns of reflectance and depths of penetrance of solar radiation differ for the coats, the considerable insulation limited the radiant heat reaching the skin. These data suggest that generally, if mammal coats have high insulation then heat flow from solar radiation into an animal is much restricted and the impact of coat colour is negligible. However, comparisons with published data from other species suggest that as fur insulation decreases, colour increasingly influences the heat inflow associated with solar radiation.

From consumption to harvest: Environmental fate prediction of excreted ionizable trace organic chemicals.

PubMed

Polesel, Fabio; Plósz, Benedek Gy; Trapp, Stefan

2015-11-01

Excreted trace organic chemicals, e.g., pharmaceuticals and biocides, typically undergo incomplete elimination in municipal wastewater treatment plants (WWTPs) and are released to surface water via treated effluents and to agricultural soils through sludge amendment and/or irrigation with freshwater or reclaimed wastewater. Recent research has shown the tendency for these substances to accumulate in food crops. In this study, we developed and applied a simulation tool to predict the fate of three ionizable trace chemicals (triclosan-TCS, furosemide-FUR, ciprofloxacin-CIP) from human consumption/excretion up to the accumulation in soil and plant, following field amendment with sewage sludge or irrigation with river water (assuming dilution of WWTP effluent). The simulation tool combines the SimpleTreat model modified for fate prediction of ionizable chemicals in a generic WWTP and a recently developed dynamic soil-plant uptake model. The simulation tool was tested using country-specific (e.g., consumption/emission rates, precipitation and temperature) input data. A Monte Carlo-based approach was adopted to account for the uncertainty associated to physico-chemical and biokinetic model parameters. Results obtained in this study suggest significant accumulation of TCS and CIP in sewage sludge (1.4-2.8 mg kgDW(-1)) as compared to FUR (0.02-0.11 mg kgDW(-1)). For the latter substance, more than half of the influent load (60.1%-72.5%) was estimated to be discharged via WWTP effluent. Specific emission rates (g ha(-1) a(-1)) of FUR to soil via either sludge application or irrigation were up to 300 times lower than for TCS and CIP. Nevertheless, high translocation potential to wheat was predicted for FUR, reaching concentrations up to 4.3 μg kgDW(-1) in grain. Irrigation was found to enhance the relative translocation of FUR to plant (45.3%-48.9% of emission to soil), as compared to sludge application (21.9%-27.6%). A comparison with peer-reviewed literature showed that model predictions were close to experimental data for elimination in WWTP, concentrations in sewage and sludge and bioconcentration factors (BCFs) in plant tissues, which showed however a large variability. The simulation tool presented here can thus be useful for priority setting and for the estimation of human exposure to trace chemicals via intake of food crops. Copyright © 2015 Elsevier Ltd. All rights reserved.

Ökophysik: Plaudereien über das Leben auf dem Land, im Wasser und in der Luft

NASA Astrophysics Data System (ADS)

Nachtigall, W.

Prof. em. Dr. rer. nat. Werner Nachtigall, geb. 1934, war als Zoophysiologe und Biophysiker Leiter des Zoologischen Instituts der Universität des Saarlandes in Saarbrücken. In Forschung und Ausbildung hat er sich insbesondere mit Aspekten der Technischen Biologie und Bionik befasst und mit seinen Forschergruppen viele Basisdaten insbesondere zur Ökologie, Physiologie und Physik des Fliegens und Schwimmens aber auch zur Stabilität beispielsweise der Gräser erarbeitet. Lebewesen überraschen immer wieder durch ihre "Biodiversität", ihre hochspezifischen Ausgestaltungen und Anpassungen.

5-FU resistant EMT-like pancreatic cancer cells are hypersensitive to photochemical internalization of the novel endoglin-targeting immunotoxin CD105-saporin.

PubMed

Lund, Kaja; Olsen, Cathrine Elisabeth; Wong, Judith Jing Wen; Olsen, Petter Angell; Solberg, Nina Therese; Høgset, Anders; Krauss, Stefan; Selbo, Pål Kristian

2017-12-19

Development of resistance to 5-fluorouracil (5-FU) is a major problem in treatment of various cancers including pancreatic cancer. In this study, we reveal important resistance mechanisms and photochemical strategies to overcome 5-FU resistance in pancreatic adenocarcinoma. 5-FU resistant (5-FUR), epithelial-to-mesenchymal-like sub-clones of the wild type pancreatic cancer cell line Panc03.27 were previously generated in our lab. We investigated the cytotoxic effect of the endosomal/lysosomal-localizing photosensitizer TPCS 2a (fimaporfin) combined with light (photochemical treatment, PCT) using MTS viability assay, and used fluorescence microscopy to show localization of TPCS 2a and to investigate the effect of photodamage of lysosomes. Flow cytometric analysis was performed to investigate uptake of photosensitizer and to assess intracellular ROS levels. Expression and localization of LAMP1 was assessed using RT-qPCR, western blotting, and structured illumination microscopy. MTS viability assay was used to assess the effect of combinations of 5-FU, chloroquine (CQ), and photochemical treatment. Expression of CD105 was investigated using RT-qPCR, western blotting, flow cytometry, and fluorescence microscopy, and co-localization of TPCS 2a and anti-CD105-saporin was assessed using microscopy. Lastly, the MTS assay was used to investigate cytotoxic effects of photochemical internalization (PCI) of the anti-CD105-immunotoxin. The 5-FUR cell lines display hypersensitivity to PCT, which was linked to increased uptake of TPCS 2a , altered lysosomal distribution, lysosomal photodamage and increased expression of the lysosomal marker LAMP-1 in the 5-FUR cells. We show that inhibition of autophagy induced by either chloroquine or lysosomal photodamage increases the sensitivity to 5-FU in the resistant cells. The three 5-FUR sub-clones overexpress Endoglin (CD105). Treatment with the immunotoxin anti-CD105-saporin alone significantly reduced the viability of the CD105-expressing 5-FUR cells, whereas little effect was seen in the CD105-negative non-resistant parental cancer cell lines. Strikingly, using the intracellular drug delivery method photochemical internalization (PCI) by combining light-controlled activation of the TPCS 2a with nanomolar levels of CD105-saporin resulted in strong cytotoxic effects in the 5-FUR cell population. Our findings suggested that autophagy is an important resistance mechanism against the chemotherapeutic drug 5-FU in pancreatic cancer cells, and that inhibition of the autophagy process, either by CQ or lysosomal photodamage, can contribute to increased sensitivity to 5-FU. For the first time, we demonstrate the promise of PCI-based targeting of CD105 in site-specific elimination of 5-FU resistant pancreatic cancer cells in vitro. In conclusion, PCI-based targeting of CD105 may represent a potent anticancer strategy and should be further evaluated in pre-clinical models.

First results with the novel petawatt laser acceleration facility in Dresden

NASA Astrophysics Data System (ADS)

Schramm, U.; Bussmann, M.; Irman, A.; Siebold, M.; Zeil, K.; Albach, D.; Bernert, C.; Bock, S.; Brack, F.; Branco, J.; Couperus, JP; Cowan, TE; Debus, A.; Eisenmann, C.; Garten, M.; Gebhardt, R.; Grams, S.; Helbig, U.; Huebl, A.; Kluge, T.; Köhler, A.; Krämer, JM; Kraft, S.; Kroll, F.; Kuntzsch, M.; Lehnert, U.; Loeser, M.; Metzkes, J.; Michel, P.; Obst, L.; Pausch, R.; Rehwald, M.; Sauerbrey, R.; Schlenvoigt, HP; Steiniger, K.; Zarini, O.

2017-07-01

We report on first commissioning results of the DRACO Petawatt ultra-short pulse laser system implemented at the ELBE center for high power radiation sources of Helmholtz-Zentrum Dresden-Rossendorf. Key parameters of the laser system essential for efficient and reproducible performance of plasma accelerators are presented and discussed with the demonstration of 40 MeV proton acceleration under TNSA conditions as well as peaked electron spectra with unprecedented bunch charge in the 0.5 nC range.

Perfluorinated contaminants in fur seal pups and penguin eggs from South Shetland, Antarctica.

PubMed

Schiavone, A; Corsolini, S; Kannan, K; Tao, L; Trivelpiece, W; Torres, D; Focardi, S

2009-06-01

Perfluorinated compounds (PFCs) have emerged as a new class of global environmental pollutants. In this study, the presence of perfluorochemicals (PFCs) in penguin eggs and Antarctic fur seals was reported for the first time. Tissue samples from Antarctic fur seal pups and penguin eggs were collected during the 2003/04 breeding season. Ten PFC contaminants were determined in seal and penguin samples. The PFC concentrations in seal liver were in the decreasing order, PFOS>PFNA>PFHpA>PFUnDA while in Adélie penguin eggs were PFHpA>PFUnDA>PFDA>PFDoDA, and in Gentoo penguin eggs were PFUnDA>PFOS>PFDoDA>PFHpA. The PFC concentrations differed significantly between seals and penguins (p<0.005) and a species-specific difference was found between the two species of penguins (p<0.005). In our study we found a mean concentration of PFOS in seal muscle and liver samples of 1.3 ng/g and 9.4 ng/g wet wt, respectively, and a mean concentration in Gentoo and Adélie penguin eggs of 0.3 ng/g and 0.38 ng/g wet wt, respectively. PFCs detected in penguin eggs and seal pups suggested oviparous and viviparous transfer of PFOS to eggs and off-springs.

Characterization of a Vibrio vulnificus LysR homologue, HupR, which regulates expression of the haem uptake outer membrane protein, HupA.

PubMed

Litwin, C M; Quackenbush, J

2001-12-01

In Vibrio vulnificus, the ability to acquire iron from the host has been shown to correlate with virulence. Here, we show that the DNA upstream of hupA (haem uptake receptor) in V. vulnificus encodes a protein in the inverse orientation to hupA (named hupR). HupR shares homology with the LysR family of positive transcriptional activators. A hupA-lacZ fusion contained on a plasmid was transformed into Fur(-), Fur(+)and HupR(-)strains of V. vulnificus. The beta-galactosidase assays and Northern blot analysis showed that transcription of hupA is negatively regulated by iron and the Fur repressor in V. vulnificus. Under low-iron conditions with added haemin, the expression of hupA in the hupR mutant was significantly lower than in the wild-type. This diminished response to haem was detected by both Northern blot and hupA-lacZ fusion analysis. The haem response of hupA in the hupR mutant was restored to wild-type levels when complemented with hupR in trans. These studies suggest that HupR may act as a positive regulator of hupA transcription under low-iron conditions in the presence of haemin. Copyright 2001 Academic Press.

Iron-regulated biofilm formation in Staphylococcus aureus Newman requires ica and the secreted protein Emp.

PubMed

Johnson, Miranda; Cockayne, Alan; Morrissey, Julie A

2008-04-01

Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface and have previously been implicated in a number of aspects of S. aureus pathogenesis. We showed here that the transcription of these important virulence factors is induced by growth in low-iron medium, reflective of the in vivo environment. Our results show that iron regulation of Eap and Emp is Fur independent. However, Fur is required for full induction of eap and emp expression in low-iron conditions. In this study, we demonstrated that in addition to Fur, low-iron-induced biofilm formation requires Sae, Agr, and SarA. In iron-restricted growth conditions, Sae and Agr are essential for Emp and Eap expression and hence for biofilm formation, whereas SarA appears to have a less-significant role. We also showed that expression of the ica operon is required for biofilm formation in iron-restricted growth conditions. We demonstrated that in fact, ica is required for the expression of the important multifunctional virulence determinants eap and emp.

Iron-Regulated Biofilm Formation in Staphylococcus aureus Newman Requires ica and the Secreted Protein Emp▿

PubMed Central

Johnson, Miranda; Cockayne, Alan; Morrissey, Julie A.

2008-01-01

Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface and have previously been implicated in a number of aspects of S. aureus pathogenesis. We showed here that the transcription of these important virulence factors is induced by growth in low-iron medium, reflective of the in vivo environment. Our results show that iron regulation of Eap and Emp is Fur independent. However, Fur is required for full induction of eap and emp expression in low-iron conditions. In this study, we demonstrated that in addition to Fur, low-iron-induced biofilm formation requires Sae, Agr, and SarA. In iron-restricted growth conditions, Sae and Agr are essential for Emp and Eap expression and hence for biofilm formation, whereas SarA appears to have a less-significant role. We also showed that expression of the ica operon is required for biofilm formation in iron-restricted growth conditions. We demonstrated that in fact, ica is required for the expression of the important multifunctional virulence determinants eap and emp. PMID:18268030

Utilization of waste of chicken feathers and waste of cardboard as the material of acoustic panel maker

NASA Astrophysics Data System (ADS)

Ansarullah; Rahim, Ramli; Kusno, Asniawaty; Baharuddin; Jamala, Nurul

2018-03-01

In The existence of chicken fur is a waste of chicken slaughterhouse Which is produced daily and still not widely used. Likewise cartons everywhere we can see its being because its function is so great in all human activities In the fulfillment of the need for storage and packaging of goods for human purposes such as electronic goods, commodity, Because it has a relatively large thickness of paper. Several studies have proven that Quill and cardboard can be used for acoustic materials. This study aims to identify the potential of chicken fur and cardboard to be created as panel materials Which acts as an acoustic panel. . This study uses an experimental method by combining two materials, Including waste Quill and carton waste by performing several stages in the formation of panels, Such as the selection of chicken fur material and cardboard cleaning process, drying process, enumeration process, panel modeling process. The result of this research is acoustic panel model with size 20x20cm2 with thickness 9 and 18 mm, The study also produced a Ø9,8 cm diameter-shaped panel model with 1.5cm, 2.5cm, and 5cm thickness for use in testing absorption coefficients using impedance tubes.

Food of the Pacific white-sided dolphin, Lagenorhynchus obliquidens, Dall's porpoise, Phocoenoides dalli, and northern fur seal, Callorhinus ursinus, off California and Washington

USGS Publications Warehouse

Stroud, Richard K.; Fiscus, Clifford H.; Kajimura, Hiroshi

1981-01-01

Our knowledge of the feeding habits of the Pacific white-sided dolphin, Lagenorhynchus obliquidens, and the Dall's porpoise, Phocoenoides dalli, is based on examination of the stomach contents of stranded animals, animals accidentally taken in commercial fishing gear, those taken in the western Pacific commercial fishery, and animals that died during capture attempts. Of these only a few were normally feeding animals taken at sea, whose stomach contents were thoroughly examined. Fished and squids previously identified from stomachs of dolphins and porpoises by various investigators are listed in Table 1.This paper documents the stomach contents of 44 Pacific white-sided dolphin and 9 Dall's porpoise collected at sea off California and Washington. All animals were collected by the authors during pelagic fur seal studies with the exception of three dolphins which were collected by a staff biologist during whale research voyages off California. Comparisons of stomach contents are made between the Pacific white-sided dolphins, Dall's porpoise, and northern fur seal, Callorhinus ursinus, collected near the same locations and usually on the same day. Mention of the dolphin, porpoise , and seal in this paper refers to the above-named species unless noted otherwise.

Cupriavidus necator JMP134 rapidly reduces furfural with a Zn-dependent alcohol dehydrogenase.

PubMed

Li, Qunrui; Metthew Lam, L K; Xun, Luying

2011-11-01

Ethanol is a renewable biofuel, and it can be produced from lignocellulosic biomass. The biomass is usually converted to hydrolysates that consist of sugar and sugar derivatives, such as furfural. Yeast ferments sugar to ethanol, but furfural higher than 3 mM is inhibitory. It can take several days for yeast cells to reduce furfural to non-inhibitory furfuryl alcohol before producing ethanol. Bioreduction of furfural to furfuryl alcohol before fermentation may relieve yeast from furfural toxicity. We observed that Cupriavidus necator JMP134, a strict aerobe, rapidly reduced 17 mM furfural to less than 3 mM within 14 min with cell turbidity of 1.0 at 600 nm at 50°C. The rapid reduction consumed ethanol. The "furfural reductase" (FurX) was purified, and it oxidized ethanol to acetaldehyde and reduced furfural to furfuryl alcohol with NAD(+) as the cofactor. The protein was identified with mass spectrometry fingerprinting to be a hypothetical protein belonging to Zn-dependent alcohol dehydrogenase family. The furX-inactivation mutant of C. necator JMP134 lost the ability to rapidly reduce furfural, and Escherichia coli producing recombinant FurX gained the ability. Thus, an alcohol dehydrogenase enabled bacteria to rapidly reduce furfural with ethanol as the reducing power.

Transmission FTIR derivative spectroscopy for estimation of furosemide in raw material and tablet dosage form

PubMed Central

Gallignani, Máximo; Rondón, Rebeca A.; Ovalles, José F.; Brunetto, María R.

2014-01-01

A Fourier transform infrared derivative spectroscopy (FTIR-DS) method has been developed for determining furosemide (FUR) in pharmaceutical solid dosage form. The method involves the extraction of FUR from tablets with N,N-dimethylformamide by sonication and direct measurement in liquid phase mode using a reduced path length cell. In general, the spectra were measured in transmission mode and the equipment was configured to collect a spectrum at 4 cm−1 resolution and a 13 s collection time (10 scans co-added). The spectra were collected between 1400 cm−1 and 450 cm−1. Derivative spectroscopy was used for data processing and quantitative measurement using the peak area of the second order spectrum of the major spectral band found at 1165 cm−1 (SO2 stretching of FUR) with baseline correction. The method fulfilled most validation requirements in the 2 mg/mL and 20 mg/mL range, with a 0.9998 coefficient of determination obtained by simple calibration model, and a general coefficient of variation <2%. The mean recovery for the proposed assay method resulted within the (100±3)% over the 80%–120% range of the target concentration. The results agree with a pharmacopoeial method and, therefore, could be considered interchangeable. PMID:26579407

Facilities for macromolecular crystallography at the Helmholtz-Zentrum Berlin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mueller, Uwe; Darowski, Nora; Fuchs, Martin R.

2012-03-20

Three macromolecular crystallography (MX) beamlines at the Helmholtz-Zentrum Berlin (HZB) are available for the regional, national and international structural biology user community. The state-of-the-art synchrotron beamlines for MX BL14.1, BL14.2 and BL14.3 are located within the low-[beta] section of the BESSY II electron storage ring. All beamlines are fed from a superconducting 7 T wavelength-shifter insertion device. BL14.1 and BL14.2 are energy tunable in the range 5-16 keV, while BL14.3 is a fixed-energy side station operated at 13.8 keV. All beamlines are equipped with CCD detectors. BL14.1 and BL14.2 are in regular user operation providing about 200 beam days permore » year and about 600 user shifts to approximately 50 research groups across Europe. BL14.3 has initially been used as a test facility and was brought into regular user mode operation during the year 2010. BL14.1 has recently been upgraded with a microdiffractometer including a mini-[kappa] goniometer and an automated sample changer. Other user facilities include office space adjacent to the beamlines, a sample preparation laboratory, a biology laboratory (safety level 1) and high-end computing resources. In this article the instrumentation of the beamlines is described, and a summary of the experimental possibilities of the beamlines and the provided ancillary equipment for the user community is given.« less

Facilities for macromolecular crystallography at the Helmholtz-Zentrum Berlin

PubMed Central

Mueller, Uwe; Darowski, Nora; Fuchs, Martin R.; Förster, Ronald; Hellmig, Michael; Paithankar, Karthik S.; Pühringer, Sandra; Steffien, Michael; Zocher, Georg; Weiss, Manfred S.

2012-01-01

Three macromolecular crystallography (MX) beamlines at the Helmholtz-Zentrum Berlin (HZB) are available for the regional, national and international structural biology user community. The state-of-the-art synchrotron beamlines for MX BL14.1, BL14.2 and BL14.3 are located within the low-β section of the BESSY II electron storage ring. All beamlines are fed from a superconducting 7 T wavelength-shifter insertion device. BL14.1 and BL14.2 are energy tunable in the range 5–16 keV, while BL14.3 is a fixed-energy side station operated at 13.8 keV. All three beamlines are equipped with CCD detectors. BL14.1 and BL14.2 are in regular user operation providing about 200 beam days per year and about 600 user shifts to approximately 50 research groups across Europe. BL14.3 has initially been used as a test facility and was brought into regular user mode operation during the year 2010. BL14.1 has recently been upgraded with a microdiffractometer including a mini-κ goniometer and an automated sample changer. Additional user facilities include office space adjacent to the beamlines, a sample preparation laboratory, a biology laboratory (safety level 1) and high-end computing resources. In this article the instrumentation of the beamlines is described, and a summary of the experimental possibilities of the beamlines and the provided ancillary equipment for the user community is given. PMID:22514183

Brillouin Spectroscopy at the GeoForschungsZentrum-Potsdam: Applications in Geosciences and Materials Science

NASA Astrophysics Data System (ADS)

Marquardt, H.; Speziale, S.; Reichmann, H. J.; Schmidt, C.; Schilling, F.

2007-12-01

Knowledge of the elastic properties of earth materials at relevant pressures and temperatures is prerequisite to adequately interpret seismic data and improve our understanding of the composition and mineralogy of the earth's interior. The study of pressure and temperature dependence of acoustic velocity in geo-fluids gives us information about their density with implications for the thermodynamic modelling of diagenetic and metamorphic processes. Furthermore elasticity of synthetic materials is of major interest for several industrial applications. We have recently completed the construction of a new Brillouin System at the GeoForschungsZentrum Potsdam. The new system has been designed to study elasticity of various geological as well as industrial materials at different pressure and temperature conditions. Our Brillouin system consists of a Nd:YVO4 solid state laser operating at a wavelength of 532 nm as light source and a Sandercock-type tandem multipass Fabry-Perot interferometer equipped with a photomultiplier tube for signal detection. Measurements can be performed in 60 or 90 degree symmetric forward scattering or in backscattering geometry. The large positioning system also allows for high temperature measurements in an externally heated diamond anvil cell. We present first results of elasticity measurements both on Earth's materials, such as silicates garnets and oxides, as well as hydroxides and commercially used garnet aluminates, and silicate and borate glasses as proxies for melts. Brillouin scattering has been measured to constrain the density of water-NaCl solutions, for the precise thermodynamic modelling of fluids in metamorphic systems.

Molecular systematics of pinniped hookworms (Nematoda: Uncinaria): species delimitation, host associations and host-induced morphometric variation.

PubMed

Nadler, Steven A; Lyons, Eugene T; Pagan, Christopher; Hyman, Derek; Lewis, Edwin E; Beckmen, Kimberlee; Bell, Cameron M; Castinel, Aurelie; Delong, Robert L; Duignan, Padraig J; Farinpour, Cher; Huntington, Kathy Burek; Kuiken, Thijs; Morgades, Diana; Naem, Soraya; Norman, Richard; Parker, Corwin; Ramos, Paul; Spraker, Terry R; Berón-Vera, Bárbara

2013-12-01

Hookworms of the genus Uncinaria have been widely reported from juvenile pinnipeds, however investigations of their systematics has been limited, with only two species described, Uncinaria lucasi from northern fur seals (Callorhinus ursinus) and Uncinaria hamiltoni from South American sea lions (Otaria flavescens). Hookworms were sampled from these hosts and seven additional species including Steller sea lions (Eumetopias jubatus), California sea lions (Zalophus californianus), South American fur seals (Arctocephalus australis), Australian fur seals (Arctocephalus pusillus), New Zealand sea lions (Phocarctos hookeri), southern elephant seals (Mirounga leonina), and the Mediterranean monk seal (Monachus monachus). One hundred and thirteen individual hookworms, including an outgroup species, were sequenced for four genes representing two loci (nuclear ribosomal DNA and mitochondrial DNA). Phylogenetic analyses of these sequences recovered seven independent evolutionary lineages or species, including the described species and five undescribed species. The molecular evidence shows that U. lucasi parasitises both C. ursinus and E. jubatus, whereas U. hamiltoni parasitises O. flavescens and A. australis. The five undescribed hookworm species were each associated with single host species (Z. californianus, A. pusillus, P. hookeri, M. leonina and M. monachus). For parasites of otarids, patterns of Uncinaria host-sharing and phylogenetic relationships had a strong biogeographic component with separate clades of parasites from northern versus southern hemisphere hosts. Comparison of phylogenies for these hookworms and their hosts suggests that the association of U. lucasi with northern fur seals results from a host-switch from Steller sea lions. Morphometric data for U. lucasi shows marked host-associated size differences for both sexes, with U. lucasi individuals from E. jubatus significantly larger. This result suggests that adult growth of U. lucasi is reduced within the host species representing the more recent host-parasite association. Intraspecific host-induced size differences are inconsistent with the exclusive use of morphometrics to delimit and diagnose species of Uncinaria from pinnipeds. Copyright © 2013 Australian Society for Parasitology Inc. All rights reserved.

Having older siblings is associated with gut microbiota development during early childhood.

PubMed

Laursen, Martin Frederik; Zachariassen, Gitte; Bahl, Martin Iain; Bergström, Anders; Høst, Arne; Michaelsen, Kim F; Licht, Tine Rask

2015-08-01

Evidence suggests that early life infections, presence of older siblings and furred pets in the household affect the risk of developing allergic diseases through altered microbial exposure. Recently, low gut microbial diversity during infancy has also been linked with later development of allergies. We investigated whether presence of older siblings, furred pets and early life infections affected gut microbial communities at 9 and 18 months of age and whether these differences were associated with the cumulative prevalence of atopic symptoms of eczema and asthmatic bronchitis at 3 years of age. Bacterial compositions and diversity indices were determined in fecal samples collected from 114 infants in the SKOT I cohort at age 9 and 18 months by 16S rRNA gene sequencing. These were compared to the presence of older siblings, furred pets and early life infections and the cumulative prevalence of diagnosed asthmatic bronchitis and self-reported eczema at 3 years of age. The number of older siblings correlated positively with bacterial diversity (p = 0.030), diversity of the phyla Firmicutes (p = 0.013) and Bacteroidetes (p = 0.004) and bacterial richness (p = 0.006) at 18 months. Further, having older siblings was associated with increased relative abundance of several bacterial taxa at both 9 and 18 months of age. Compared to the effect of having siblings, presence of household furred pets and early life infections had less pronounced effects on the gut microbiota. Gut microbiota characteristics were not significantly associated with cumulative occurrence of eczema and asthmatic bronchitis during the first 3 years of life. Presence of older siblings is associated with increased gut microbial diversity and richness during early childhood, which could contribute to the substantiation of the hygiene hypothesis. However, no associations were found between gut microbiota and atopic symptoms of eczema and asthmatic bronchitis during early childhood and thus further studies are required to elucidate whether sibling-associated gut microbial changes influence development of allergies later in childhood.

Identification of a lineage specific zinc responsive genomic island in Mycobacterium avium ssp. paratuberculosis.

PubMed

Eckelt, Elke; Jarek, Michael; Frömke, Cornelia; Meens, Jochen; Goethe, Ralph

2014-12-06

Maintenance of metal homeostasis is crucial in bacterial pathogenicity as metal starvation is the most important mechanism in the nutritional immunity strategy of host cells. Thus, pathogenic bacteria have evolved sensitive metal scavenging systems to overcome this particular host defence mechanism. The ruminant pathogen Mycobacterium avium ssp. paratuberculosis (MAP) displays a unique gut tropism and causes a chronic progressive intestinal inflammation. MAP possesses eight conserved lineage specific large sequence polymorphisms (LSP), which distinguish MAP from its ancestral M. avium ssp. hominissuis or other M. avium subspecies. LSP14 and LSP15 harbour many genes proposed to be involved in metal homeostasis and have been suggested to substitute for a MAP specific, impaired mycobactin synthesis. In the present study, we found that a LSP14 located putative IrtAB-like iron transporter encoded by mptABC was induced by zinc but not by iron starvation. Heterologous reporter gene assays with the lacZ gene under control of the mptABC promoter in M. smegmatis (MSMEG) and in a MSMEG∆furB deletion mutant revealed a zinc dependent, metalloregulator FurB mediated expression of mptABC via a conserved mycobacterial FurB recognition site. Deep sequencing of RNA from MAP cultures treated with the zinc chelator TPEN revealed that 70 genes responded to zinc limitation. Remarkably, 45 of these genes were located on a large genomic island of approximately 90 kb which harboured LSP14 and LSP15. Thirty-five of these genes were predicted to be controlled by FurB, due to the presence of putative binding sites. This clustering of zinc responsive genes was exclusively found in MAP and not in other mycobacteria. Our data revealed a particular genomic signature for MAP given by a unique zinc specific locus, thereby suggesting an exceptional relevance of zinc for the metabolism of MAP. MAP seems to be well adapted to maintain zinc homeostasis which might contribute to the peculiarity of MAP pathogenicity.

Comparison of Flexible Ureterorenoscope Quality of Vision: An In Vitro Study.

PubMed

Talso, Michele; Proietti, Silvia; Emiliani, Esteban; Gallioli, Andrea; Dragos, Laurian; Orosa, Andrea; Servian, Pol; Barreiro, Aaron; Giusti, Guido; Montanari, Emanuele; Somani, Bhaskar; Traxer, Olivier

2018-06-01

Flexible ureterorenoscopy (fURS) is one of the best solutions for treatment of renal calculi <2 cm and for upper urinary tract urothelial carcinoma conservative treatment. An adequate quality of vision is mandatory to help surgeon get better outcomes. No studies have been done, to our knowledge, about what fURS in the market has the best quality of vision. Seven different fURS were used to compare the image quality (Lithovue, Olympus V, Olympus V2, Storz Flex XC-in White Light and in Clara+Chroma mode-Wolf Cobra Vision, Olympus P6, and Storx Flex X2). Two standardized grids to evaluate contrast and image definition and three stones of different composition were filmed in four standardized different scenarios. These videos were shown to 103 subjects (51 urologists and 52 nonurologists) who had to evaluate them with a rating scale from 1 (very bad) to 5 (very good). No difference in terms of scores was observed for sex of the participants. Digital (D) ureterorenoscopes were rated better than fiber optics (FOs) ureterorenoscopes. Overall, Flex XC White Light and XC Clara+Chroma image quality resulted steadily better than other fURS (p < 0.0001). Olympus V generally provided a vision better than Lithovue. Cobra Vision and Olympus V2 had superimposable values that were significantly lower than Lithovue's ones. Olympus P6 and Storz X2 offered a low quality of vision compared to the others. In the medium simulating bleeding, Olympus V and V2 significantly improved their scores of 12% and 8.1%, contrary to rest of the ureterorenoscopes. D ureterorenoscopes have a better image quality than FO ones. The only disposable ureterorenoscope tested was comparable to the majority of other D ureterorenoscopes. The best image quality was provided by Storz D ureterorenoscopes, being Clara Chroma the favorite Spies Mode, according to literature.

Benefits of a Ball and Chain: Simple Environmental Enrichments Improve Welfare and Reproductive Success in Farmed American Mink (Neovison vison)

PubMed Central

Meagher, Rebecca K.; Ahloy Dallaire, Jamie; Campbell, Dana L. M.; Ross, Misha; Møller, Steen H.; Hansen, Steffen W.; Díez-León, María; Palme, Rupert; Mason, Georgia J.

2014-01-01

Can simple enrichments enhance caged mink welfare? Pilot data from 756 sub-adults spanning three colour-types (strains) identified potentially practical enrichments, and suggested beneficial effects on temperament and fur-chewing. Our main experiment started with 2032 Black mink on three farms: from each of 508 families, one juvenile male-female pair was enriched (E) with two balls and a hanging plastic chain or length of hose, while a second pair was left as a non-enriched (NE) control. At 8 months, more than half the subjects were killed for pelts, and 302 new females were recruited (half enriched: ‘late E’). Several signs of improved welfare or productivity emerged. Access to enrichment increased play in juveniles. E mink were calmer (less aggressive in temperament tests; quieter when handled; less fearful, if male), and less likely to fur-chew, although other stereotypic behaviours were not reduced. On one farm, E females had lower cortisol (inferred from faecal metabolites). E males tended to copulate for longer. E females also weaned more offspring: about 10% more juveniles per E female, primarily caused by reduced rates of barrenness (‘late E’ females also giving birth to bigger litters on one farm), effects that our data cautiously suggest were partly mediated by reduced inactivity and changes in temperament. Pelt quality seemed unaffected, but E animals had cleaner cages. In a subsidiary side-study using 368 mink of a second colour-type (‘Demis’), similar temperament effects emerged, and while E did not reduce fur-chewing or improve reproductive success in this colour-type, E animals were judged to have better pelts. Overall, simple enrichments were thus beneficial. These findings should encourage welfare improvements on fur farms (which house 60-70 million mink p.a.) and in breeding centres where endangered mustelids (e.g. black-footed ferrets) often reproduce poorly. They should also stimulate future research into more effective practical enrichments. PMID:25386726

Benefits of a ball and chain: simple environmental enrichments improve welfare and reproductive success in farmed American mink (Neovison vison).

PubMed

Meagher, Rebecca K; Ahloy Dallaire, Jamie; Campbell, Dana L M; Ross, Misha; Møller, Steen H; Hansen, Steffen W; Díez-León, María; Palme, Rupert; Mason, Georgia J

2014-01-01

Can simple enrichments enhance caged mink welfare? Pilot data from 756 sub-adults spanning three colour-types (strains) identified potentially practical enrichments, and suggested beneficial effects on temperament and fur-chewing. Our main experiment started with 2032 Black mink on three farms: from each of 508 families, one juvenile male-female pair was enriched (E) with two balls and a hanging plastic chain or length of hose, while a second pair was left as a non-enriched (NE) control. At 8 months, more than half the subjects were killed for pelts, and 302 new females were recruited (half enriched: 'late E'). Several signs of improved welfare or productivity emerged. Access to enrichment increased play in juveniles. E mink were calmer (less aggressive in temperament tests; quieter when handled; less fearful, if male), and less likely to fur-chew, although other stereotypic behaviours were not reduced. On one farm, E females had lower cortisol (inferred from faecal metabolites). E males tended to copulate for longer. E females also weaned more offspring: about 10% more juveniles per E female, primarily caused by reduced rates of barrenness ('late E' females also giving birth to bigger litters on one farm), effects that our data cautiously suggest were partly mediated by reduced inactivity and changes in temperament. Pelt quality seemed unaffected, but E animals had cleaner cages. In a subsidiary side-study using 368 mink of a second colour-type ('Demis'), similar temperament effects emerged, and while E did not reduce fur-chewing or improve reproductive success in this colour-type, E animals were judged to have better pelts. Overall, simple enrichments were thus beneficial. These findings should encourage welfare improvements on fur farms (which house 60-70 million mink p.a.) and in breeding centres where endangered mustelids (e.g. black-footed ferrets) often reproduce poorly. They should also stimulate future research into more effective practical enrichments.

Effects of Dietary Copper and Zinc Supplementation on Growth Performance, Tissue Mineral Retention, Antioxidant Status, and Fur Quality in Growing-Furring Blue Foxes (Alopex lagopus).

PubMed

Liu, Zhi; Wu, Xuezhuang; Zhang, Tietao; Guo, Jungang; Gao, Xiuhua; Yang, Fuhe; Xing, Xiumei

2015-12-01

A 4×2 factorial experiment with four supplemental levels of copper (0, 20, 40, or 60 mg copper per kg dry matter) from copper sulfate and two supplemental levels of zinc (40 or 200 mg zinc per kg dry matter) from zinc sulfate was conducted to investigate the effects of dietary copper and zinc supplementation on growth performance, tissue mineral retention, antioxidant status, and fur quality in growing-furring blue foxes. One hundred and twenty healthy 15-week-old male blue foxes were randomly allocated to eight dietary treatments with 15 replicates per treatment for a 70-day trial from mid-September to pelting in December. The average daily gain and feed conversion ratio were increased with copper supplementation in the first 35 days as well as the overall period (P<0.05). In addition, copper supplementation tended to increase feed intake during the first 35 days (P<0.10). Diets supplemented with 200 mg/kg zinc did not affect body gain (P>0.10) and feed intake (P>0.10) but improved feed conversion (P<0.05) compared with those supplemented 40 mg/kg zinc throughout the experiment. No copper×zinc interaction was observed for growth performance except that a tendency (P=0.09) was found for feed intake in the first 35 days. Supplementation of copper or zinc improved crude fat digestibility (P<0.01) but had no effects on the digestibility of other nutrients. Fecal copper was increased with both copper (P<0.01) and zinc addition (P<0.05). However, fecal zinc was affected only by dietary zinc addition (P<0.01). Mineral contents in serum and kidney were not affected by dietary treatments (P>0.05). However, the level of copper in the liver was increased with copper supplementation (P<0.05) and tended to decrease with zinc supplementation (P=0.08). Dietary zinc addition tended to increase the activity of alkaline phosphatase (P=0.07). The activities of copper-zinc superoxide dismutase and catalase tended to increase by copper (P=0.08) and zinc addition (P=0.05). Moreover, a copper×zinc interaction was observed for catalase in the experiment (P<0.05). Serum malondialdehyde concentration decreased with the increasing of dietary copper and zinc levels (P<0.05). The activity of glutathione peroxidase tended to increase by copper addition (P=0.09). For fur quality, foxes fed diets supplemented with high copper had larger skin length and darker pelts than those fed the basal diet without copper addition (P<0.05). In conclusion, this study demonstrated that dietary copper and zinc supplementation can improve growth by increasing feed intake and improving fat digestibility. Additionally, copper and zinc can enhance the antioxidant capacity of blue foxes. This study also indicates that additional zinc up to 200 mg/kg did not exert significant adverse effects on the copper metabolism of growing-furring blue foxes.

48 CFR 25.104 - Nonavailable articles.

Code of Federal Regulations, 2013 CFR

2013-10-01

.... Bananas. Bauxite. Beef, corned, canned. Beef extract. Bephenium hydroxynapthoate. Bismuth. Books, trade..., or cast bars. Pyrethrum flowers. Quartz crystals. Quebracho. Quinidine. Quinine. Rabbit fur felt...

48 CFR 25.104 - Nonavailable articles.

Code of Federal Regulations, 2011 CFR

2011-10-01

.... Bananas. Bauxite. Beef, corned, canned. Beef extract. Bephenium hydroxynapthoate. Bismuth. Books, trade..., or cast bars. Pyrethrum flowers. Quartz crystals. Quebracho. Quinidine. Quinine. Rabbit fur felt...

48 CFR 25.104 - Nonavailable articles.

Code of Federal Regulations, 2014 CFR

2014-10-01

.... Bananas. Bauxite. Beef, corned, canned. Beef extract. Bephenium hydroxynapthoate. Bismuth. Books, trade..., or cast bars. Pyrethrum flowers. Quartz crystals. Quebracho. Quinidine. Quinine. Rabbit fur felt...

48 CFR 25.104 - Nonavailable articles.

Code of Federal Regulations, 2012 CFR

2012-10-01

.... Bananas. Bauxite. Beef, corned, canned. Beef extract. Bephenium hydroxynapthoate. Bismuth. Books, trade..., or cast bars. Pyrethrum flowers. Quartz crystals. Quebracho. Quinidine. Quinine. Rabbit fur felt...

48 CFR 25.104 - Nonavailable articles.

Code of Federal Regulations, 2010 CFR

2010-10-01

.... Bananas. Bauxite. Beef, corned, canned. Beef extract. Bephenium hydroxynapthoate. Bismuth. Books, trade..., or cast bars. Pyrethrum flowers. Quartz crystals. Quebracho. Quinidine. Quinine. Rabbit fur felt...

S-Layer Based Bio-Imprinting - Synthetic S-Layer Polymers

DTIC Science & Technology

2015-07-09

PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ORGANIZATION . 1.  REPORT DATE (DD-MM-YYYY)      14-07-2015 2.  REPORT TYPE      Final Performance 3.  DATES...NUMBER 7.  PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) ZENTRUM FUER NANOBIOTECHNOLOGIE GREGOR-MENDEL-STRASSE 33 WIEN, 1180 AT 8.  PERFORMING... ORGANIZATION      REPORT NUMBER 9.  SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES) AF Office of Scientific Research 875 N. Randolph St. Room 3112

Recent and future liquid metal experiments on homogeneous dynamo action and magnetic instabilities

NASA Astrophysics Data System (ADS)

Stefani, Frank; Gerbeth, Gunter; Giesecke, Andre; Gundrum, Thomas; Kirillov, Oleg; Seilmayer, Martin; Gellert, Marcus; Rüdiger, Günther; Gailitis, Agris

2011-10-01

The present status of the Riga dynamo experiment is summarized and the prospects for its future exploitation are evaluated. We further discuss the plans for a large-scale precession driven dynamo experiment to be set-up in the framework of the new installation DRESDYN (DREsden Sodium facility for dynamo and thermohydraulic studies) at Helmholtz-Zentrum Dresden-Rossendorf. We report recent investigations of the magnetorotational instability and the Tayler instability and sketch the plans for another large-scale liquid sodium facility devoted to the combined study of both effects.

Kelp and dolphin gulls cause perineal wounds in South American fur seal pups (Arctocephalus australis) at Guafo Island, Chilean Patagonia

PubMed Central

Muñoz, Francisco; Montalva, Felipe; Perez-Venegas, Diego; Pavés, Héctor; Gottdenker, Nicole

2017-01-01

During five reproductive seasons, we documented the presence, extent and origin of perineal wounds in South American fur seal pups (Arctocephalus australis) on Guafo Island, Northern Chilean Patagonia. The seasonal prevalence of perineal wounds ranged from 5 to 9%, and new cases were more common at the end of the breeding season (February), when pups were on average two months old and were actively expelling hookworms (Uncinaria sp). Histologically, wounds corresponded to marked ulcerative lymphoplasmacytic and histiocytic dermatitis with granulation tissue and mixed bacterial colonies. In 2015 and 2017, kelp gulls (Larus dominicanus) and dolphin gulls (Leucophaeus scoresbii) were observed picking and wounding the perineal area of marked pups. This behaviour occurred more frequently after the pups' defecation, when sea gulls engaged in consumption of pups' faeces. The affected pups usually had moderate to marked hookworm infections along with bloody diarrhoea and anaemia. Pups with severe wounds (23% of affected animals) had swollen perineal areas and signs of secondary systemic bacterial infection. We propose that seagulls on Guafo Island have learned to consume remains of blood and parasites in the faeces of pups affected by hookworm infection, causing perineal wounds during this process. We conclude that this perineal wounding is an unintentional, occasional negative effect of an otherwise commensal gull–fur seal relationship. PMID:28791178

Kelp and dolphin gulls cause perineal wounds in South American fur seal pups (Arctocephalus australis) at Guafo Island, Chilean Patagonia.

PubMed

Seguel, Mauricio; Muñoz, Francisco; Montalva, Felipe; Perez-Venegas, Diego; Pavés, Héctor; Gottdenker, Nicole

2017-07-01

During five reproductive seasons, we documented the presence, extent and origin of perineal wounds in South American fur seal pups ( Arctocephalus australis ) on Guafo Island, Northern Chilean Patagonia. The seasonal prevalence of perineal wounds ranged from 5 to 9%, and new cases were more common at the end of the breeding season (February), when pups were on average two months old and were actively expelling hookworms ( Uncinaria sp). Histologically, wounds corresponded to marked ulcerative lymphoplasmacytic and histiocytic dermatitis with granulation tissue and mixed bacterial colonies. In 2015 and 2017, kelp gulls ( Larus dominicanus ) and dolphin gulls ( Leucophaeus scoresbii ) were observed picking and wounding the perineal area of marked pups. This behaviour occurred more frequently after the pups' defecation, when sea gulls engaged in consumption of pups' faeces. The affected pups usually had moderate to marked hookworm infections along with bloody diarrhoea and anaemia. Pups with severe wounds (23% of affected animals) had swollen perineal areas and signs of secondary systemic bacterial infection. We propose that seagulls on Guafo Island have learned to consume remains of blood and parasites in the faeces of pups affected by hookworm infection, causing perineal wounds during this process. We conclude that this perineal wounding is an unintentional, occasional negative effect of an otherwise commensal gull-fur seal relationship.

Foraging-Based Enrichment Promotes More Varied Behaviour in Captive Australian Fur Seals (Arctocephalus pusillus doriferus)

PubMed Central

Hocking, David P.; Salverson, Marcia; Evans, Alistair R.

2015-01-01

During wild foraging, Australian fur seals (Arctocephalus pusillus doriferus) encounter many different types of prey in a wide range of scenarios, yet in captive environments they are typically provided with a narrower range of opportunities to display their full repertoire of behaviours. This study aimed to quantitatively explore the effect of foraging-based enrichment on the behaviour and activity patterns displayed by two captive Australian fur seals at Melbourne Zoo, Australia. Food was presented as a scatter in open water, in a free-floating ball device, or in a static box device, with each treatment separated by control trials with no enrichment. Both subjects spent more time interacting with the ball and static box devices than the scatter feed. The total time spent pattern swimming was reduced in the enrichment treatments compared to the controls, while the time spent performing random swimming behaviours increased. There was also a significant increase in the total number of bouts of behaviour performed in all three enrichment treatments compared to controls. Each enrichment method also promoted a different suit of foraging behaviours. Hence, rather than choosing one method, the most effective way to increase the diversity of foraging behaviours, while also increasing variation in general activity patterns, is to provide seals with a wide range of foraging scenarios where food is encountered in different ways. PMID:25946412

Colony-level assessment of Brucella and Leptospira in the Guadalupe fur seal, Isla Guadalupe, Mexico.

PubMed

Ziehl-Quirós, E Carolina; García-Aguilar, María C; Mellink, Eric

2017-01-24

The relatively small population size and restricted distribution of the Guadalupe fur seal Arctocephalus townsendi could make it highly vulnerable to infectious diseases. We performed a colony-level assessment in this species of the prevalence and presence of Brucella spp. and Leptospira spp., pathogenic bacteria that have been reported in several pinniped species worldwide. Forty-six serum samples were collected in 2014 from pups at Isla Guadalupe, the only place where the species effectively reproduces. Samples were tested for Brucella using 3 consecutive serological tests, and for Leptospira using the microscopic agglutination test. For each bacterium, a Bayesian approach was used to estimate prevalence to exposure, and an epidemiological model was used to test the null hypothesis that the bacterium was present in the colony. No serum sample tested positive for Brucella, and the statistical analyses concluded that the colony was bacterium-free with a 96.3% confidence level. However, a Brucella surveillance program would be highly recommendable. Twelve samples were positive (titers 1:50) to 1 or more serovars of Leptospira. The prevalence was calculated at 27.1% (95% credible interval: 15.6-40.3%), and the posterior analyses indicated that the colony was not Leptospira-free with a 100% confidence level. Serovars Icterohaemorrhagiae, Canicola, and Bratislava were detected, but only further research can unveil whether they affect the fur seal population.

Failure to produce conditioning with low-dose trimethylthiazoline or cat feces as unconditioned stimuli.

PubMed

Blanchard, D Caroline; Markham, Chris; Yang, Mu; Hubbard, David; Madarang, Eric; Blanchard, Robert J

2003-04-01

Trimethylthiazoline (TMT), a derivative of fox feces, has been reported to fail to produce aversive conditioning as an unconditioned stimulus (UCS) when presented in large amounts (I. S. McGregor, L. Schrama, P. Ambermoon, & R. A. Dielenberg, 2002). Experiment I evaluated very low TMT levels that nonetheless produced defensive behaviors in rats during exposure. Although each level (0.01, 0.05, and 0.10 microl TMT) produced significant change in defensiveness, none resulted in significant changes the following day in the absence of TMT. Experiment 2 evaluated cat urine, cat feces, and cat fur/skin odor against a no-odor control. Urine produced no significant changes, but feces and fur/skin odors elicited virtually identical changes in defensive behaviors during exposure. When tested the next day in the absence of odor, the fur/skin odor-exposed group showed significant differences on the same behaviors as during exposure, but the feces-exposed group showed no differences on any measure. Results suggest that lack of conditioning to TMT may relate to the type of predator odor rather than the amount, predator species, or possible lack of odor components in TMT that are present in natural feces. Predator feces may also be less effective as a UCS because they are poorly predictive of the actual presence of the predator, suggesting the need for a reevaluation of UCS functions in aversive conditioning.

PCR Testing of a Ventilated Caging System to Detect Murine Fur Mites

PubMed Central

Jensen, Eric S; Allen, Kenneth P; Henderson, Kenneth S; Szabo, Aniko; Thulin, Joseph D

2013-01-01

Rodents housed in microisolation caging are commonly monitored for infectious agents by the use of soiled bedding sentinels. This strategy relies on the successful transmission of rodent pathogens from the index rodents via soiled bedding to sentinel cages and the subsequent infection or colonization of sentinel rodents. When the prevalence of a pathogen is low or the target agent is not readily transmitted by soiled bedding, alternative testing methodologies should be used. Given the continued prevalence of institutions self-reporting murine fur mites and with the advent of a new sensitive and specific PCR assay for mites, we sought to determine whether the exhaust system of an individual ventilated caging (IVC) system could be used for monitoring the rack's rodent population for mites rather than relying on the responses of sentinels. We deployed single cages of mice (Mus musculus) that were known to be infested with either Radfordia affinis or Myobia musculi on a 70-cage rack, sampled the horizontal exhaust manifolds weekly, and used the new PCR assay to test these samples for mite DNA. We detected the presence of fur mites at a 94.1% probability of detection within 4 wk of placement. Therefore, we recommend swabbing and testing the shelf exhaust manifolds of IVC racks rather than relying on soiled-bedding sentinels as an indicator of the mite status of the rodents on that rack. PMID:23562030

Impairment of ntcA gene revealed its role in regulating iron homeostasis, ROS production and cellular phenotype under iron deficiency in cyanobacterium Anabaena sp. PCC 7120.

PubMed

Kaushik, Manish Singh; Srivastava, Meenakshi; Singh, Anumeha; Mishra, Arun Kumar

2017-08-01

Iron deficiency ends up into several unavoidable consequences including damaging oxidative stress in cyanobacteria. NtcA is a global nitrogen regulator controls wide range of metabolisms in addition to regulation of nitrogen metabolism. In present communication, NtcA based regulation of iron homeostasis, ROS production and cellular phenotype under iron deficiency in Anabaena 7120 has been investigated. NtcA regulates the concentration dependent iron uptake by controlling the expression of furA gene. NtcA also regulated pigment synthesis and phenotypic alterations in Anabaena 7120. A significant increase in ROS production and corresponding reduction in the activities of antioxidative enzymes (SOD, CAT, APX and GR) in CSE2 mutant strain in contrast to wild type Anabaena 7120 also suggested the possible involvement of NtcA in protection against oxidative stress in iron deficiency. NtcA has no impact on the expression of furB and furC in spite of presence of consensus NtcA binding site (NBS) and -10 boxes in their promoter. NtcA also regulates the thylakoid arrangement as well as related photosynthetic and respiration rates under iron deficiency in Anabaena 7120. Overall results suggested that NtcA regulates iron acquisition and in turn protect Anabaena cells from the damaging effects of oxidative stress induced under iron deficiency.

50 CFR 218.230 - Specified activity, level of taking, and species.

Code of Federal Regulations, 2012 CFR

2012-10-01

...), northern elephant seal (Mirounga angustirostris), northern fur seal (Callorhinus ursinus), ribbon seal... seal (Arctocephalus australis), South American sea lion (Otaria flavescens), southern elephant seal...

50 CFR 218.230 - Specified activity, level of taking, and species.

Code of Federal Regulations, 2014 CFR

2014-10-01

...), northern elephant seal (Mirounga angustirostris), northern fur seal (Callorhinus ursinus), ribbon seal... seal (Arctocephalus australis), South American sea lion (Otaria flavescens), southern elephant seal...

76 FR 46279 - Marine Mammals; File No. 87-1851

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-02

... (Lobodon carcinophagus), 35 southern elephant seals (Mirounga leonina), 10 leopard seals (Hydrurga leptonyx... elephant seals (Mirounga augustirostris), and northern fur seals (Callorhinus ursinus) in California is...

50 CFR 218.230 - Specified activity, level of taking, and species.

Code of Federal Regulations, 2013 CFR

2013-10-01

...), northern elephant seal (Mirounga angustirostris), northern fur seal (Callorhinus ursinus), ribbon seal... seal (Arctocephalus australis), South American sea lion (Otaria flavescens), southern elephant seal...

16 CFR 301.0 - Fur products name guide.

Code of Federal Regulations, 2014 CFR

2014-01-01

... Insectivora Talpidae Desmana moschata and Galemys pyrenaicus. Dog Carnivora Canidae Canis familiaris. Ermine... Mustelidae Mustela altaica and Mustela rixosa. Wolf ......do Canidae Canis lupus and Canis niger. Wolverine...

16 CFR 301.0 - Fur products name guide.

Code of Federal Regulations, 2013 CFR

2013-01-01

... Insectivora Talpidae Desmana moschata and Galemys pyrenaicus. Dog Carnivora Canidae Canis familiaris. Ermine... Mustelidae Mustela altaica and Mustela rixosa. Wolf ......do Canidae Canis lupus and Canis niger. Wolverine...

16 CFR 301.0 - Fur products name guide.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Insectivora Talpidae Desmana moschata and Galemys pyrenaicus. Dog Carnivora Canidae Canis familiaris. Ermine... Mustelidae Mustela altaica and Mustela rixosa. Wolf ......do Canidae Canis lupus and Canis niger. Wolverine...

16 CFR 301.0 - Fur products name guide.

Code of Federal Regulations, 2012 CFR

2012-01-01

... Insectivora Talpidae Desmana moschata and Galemys pyrenaicus. Dog Carnivora Canidae Canis familiaris. Ermine... Mustelidae Mustela altaica and Mustela rixosa. Wolf ......do Canidae Canis lupus and Canis niger. Wolverine...

16 CFR 301.0 - Fur products name guide.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Insectivora Talpidae Desmana moschata and Galemys pyrenaicus. Dog Carnivora Canidae Canis familiaris. Ermine... Mustelidae Mustela altaica and Mustela rixosa. Wolf ......do Canidae Canis lupus and Canis niger. Wolverine...

Velocity-based movement modeling for individual and population level inference

USGS Publications Warehouse

Hanks, Ephraim M.; Hooten, Mevin B.; Johnson, Devin S.; Sterling, Jeremy T.

2011-01-01

Understanding animal movement and resource selection provides important information about the ecology of the animal, but an animal's movement and behavior are not typically constant in time. We present a velocity-based approach for modeling animal movement in space and time that allows for temporal heterogeneity in an animal's response to the environment, allows for temporal irregularity in telemetry data, and accounts for the uncertainty in the location information. Population-level inference on movement patterns and resource selection can then be made through cluster analysis of the parameters related to movement and behavior. We illustrate this approach through a study of northern fur seal (Callorhinus ursinus) movement in the Bering Sea, Alaska, USA. Results show sex differentiation, with female northern fur seals exhibiting stronger response to environmental variables.

Probiotic Bacteria Induce a ‘Glow of Health’

PubMed Central

Smillie, Christopher; Varian, Bernard J.; Ibrahim, Yassin M.; Lakritz, Jessica R.; Alm, Eric J.; Erdman, Susan E.

2013-01-01

Radiant skin and hair are universally recognized as indications of good health. However, this ‘glow of health’ display remains poorly understood. We found that feeding of probiotic bacteria to aged mice induced integumentary changes mimicking peak health and reproductive fitness characteristic of much younger animals. Eating probiotic yogurt triggered epithelial follicular anagen-phase shift with sebocytogenesis resulting in thick lustrous fur due to a bacteria-triggered interleukin-10-dependent mechanism. Aged male animals eating probiotics exhibited increased subcuticular folliculogenesis, when compared with matched controls, yielding luxuriant fur only in probiotic-fed subjects. Female animals displayed probiotic-induced hyperacidity coinciding with shinier hair, a feature that also aligns with fertility in human females. Together these data provide insights into mammalian evolution and novel strategies for integumentary health. PMID:23342023

Respiratory Allergens from Furred Mammals: Environmental and Occupational Exposure

PubMed Central

Raulf, Monika

2017-01-01

Furry mammals kept as pets, farm and laboratory animals are important allergen sources. The prevalence of sensitization to furred mammals appears to be increasing worldwide. Several mammalian allergens from diverse species are well characterized with regard to their molecular structure and immunogenicity, and some are already available for component-resolved allergy diagnostics. The distribution of various mammalian allergens has been extensively studied during the past few decades. Animal allergens were found to be ubiquitous in the human environment, even in places where no animals reside, with concentrations differing considerably between locations and geographical regions. This review presents an overview of identified mammalian respiratory allergens classified according to protein families, and compiles the results of allergen exposure assessment studies conducted in different public and occupational environments. PMID:29056697

Velocity-Based Movement Modeling for Individual and Population Level Inference

PubMed Central

Hanks, Ephraim M.; Hooten, Mevin B.; Johnson, Devin S.; Sterling, Jeremy T.

2011-01-01

Understanding animal movement and resource selection provides important information about the ecology of the animal, but an animal's movement and behavior are not typically constant in time. We present a velocity-based approach for modeling animal movement in space and time that allows for temporal heterogeneity in an animal's response to the environment, allows for temporal irregularity in telemetry data, and accounts for the uncertainty in the location information. Population-level inference on movement patterns and resource selection can then be made through cluster analysis of the parameters related to movement and behavior. We illustrate this approach through a study of northern fur seal (Callorhinus ursinus) movement in the Bering Sea, Alaska, USA. Results show sex differentiation, with female northern fur seals exhibiting stronger response to environmental variables. PMID:21931584

Gratings for synchrotron and FEL beamlines: a project for the manufacture of ultra-precise gratings at Helmholtz Zentrum Berlin.

PubMed

Siewert, F; Löchel, B; Buchheim, J; Eggenstein, F; Firsov, A; Gwalt, G; Kutz, O; Lemke, St; Nelles, B; Rudolph, I; Schäfers, F; Seliger, T; Senf, F; Sokolov, A; Waberski, Ch; Wolf, J; Zeschke, T; Zizak, I; Follath, R; Arnold, T; Frost, F; Pietag, F; Erko, A

2018-01-01

Blazed gratings are of dedicated interest for the monochromatization of synchrotron radiation when a high photon flux is required, such as, for example, in resonant inelastic X-ray scattering experiments or when the use of laminar gratings is excluded due to too high flux densities and expected damage, for example at free-electron laser beamlines. Their availability became a bottleneck since the decommissioning of the grating manufacture facility at Carl Zeiss in Oberkochen. To resolve this situation a new technological laboratory was established at the Helmholtz Zentrum Berlin, including instrumentation from Carl Zeiss. Besides the upgraded ZEISS equipment, an advanced grating production line has been developed, including a new ultra-precise ruling machine, ion etching technology as well as laser interference lithography. While the old ZEISS ruling machine GTM-6 allows ruling for a grating length up to 170 mm, the new GTM-24 will have the capacity for 600 mm (24 inch) gratings with groove densities between 50 lines mm -1 and 1200 lines mm -1 . A new ion etching machine with a scanning radiofrequency excited ion beam (HF) source allows gratings to be etched into substrates of up to 500 mm length. For a final at-wavelength characterization, a new reflectometer at a new Optics beamline at the BESSY-II storage ring is under operation. This paper reports on the status of the grating fabrication, the measured quality of fabricated items by ex situ and in situ metrology, and future development goals.

Kids' Quest: ADHD

MedlinePlus

... turns interrupt others Return to Steps World-Wide Web Search Fin, Fur and Feather Bureau of Investigation ... outside this website We provide links to other web pages if you want to learn more about ...

76 FR 59663 - Marine Mammals; File No. 16472

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-27

... fur seals (Arctocephalus gazella), southern elephant seals (Mirounga leonina), crabeater seals... carcasses may be imported from the Antarctic. The permit is valid for five years. In compliance with the...

Related Medical Conditions

MedlinePlus

... age of 5 years... Airborne allergens (e.g., pollen) may contribute to eczema flares in older children ... the nose. Most commonly associated with allergies to pollen, dust, and/or furred animals, its symptoms include ...

Luftqualität

NASA Astrophysics Data System (ADS)

Schultz, Martin G.; Klemp, Dieter; Wahner, Andreas

Die Qualität der Luft beeinflusst in besonderer Weise die menschliche Gesundheit und hat auch Auswirkungen auf die Landwirtschaft und Ökosysteme. Viele Luftschadstoffe absorbieren oder streuen zudem die Sonnen- oder Wärmestrahlung und sind daher klimawirksam. Luftchemische Prozesse hängen, ebenso wie die Emissionen, von klimatischen Faktoren wie Sonneneinstrahlung, Temperatur und Niederschlag ab. Deshalb ist zu erwarten, dass die projizierten Klimaänderungen für Deutschland auch die Luftschadstoffkonzentrationen beeinflussen werden, auch wenn dieser Zusammenhang noch nicht gut erforscht ist. Dieses Kapitel vermittelt einen Überblick über die Zusammenhänge und weist zumindest qualitativ auf mögliche künftige Entwicklungen hin. Im Vordergrund stehen die Entwicklungen bei Feinstaub und Ozon.

Translocated sea otter populations off the coasts of Oregon and Washington

USGS Publications Warehouse

Jameson, Ronald J.; Mac, Michael J.; Opler, Paul A.; Puckett Haecker, Catherine E.; Doran, Peter D.

1998-01-01

The historical distribution of sea otters extended from the northern islands of Japan north and east across the Aleutian chain to the mainland of North America then south along the west coast to central Baja California, Mexico (Riedman and Estes 1990). By the beginning of the twentieth century, after 150 years of being intensively hunted for their valuable fur, sea otters had been extirpated from most of their range (Kenyon 1969). In 1911 sea otters were protected by the passage of the International Fur Seal Treaty. Unfortunately, only 13 remnant populations survived the fur-hunting period, and two of those, British Columbia and Mexico, would also ultimately disappear, leaving only a small group of sea otters south of Alaska, along the rugged Big Sur coast of California (Kenyon 1969).The earliest attempts to reestablish sea otters to unoccupied habitat were begun in the early 1950’s by R. D. (Sea Otter) Jones, then manager of the Aleutian National Wildlife Refuge (Kenyon 1969). These early efforts were experimental, and all failed to establish populations. However, the knowledge gained from Jones’s efforts and the seminal work of Kenyon (1969) and others during the 1950’s and early 1960’s ultimately led to the successful efforts to come.During the mid-1960’s the Alaska Department of Fish and Game began translocating sea otters to sites where the species had occurred before the fur-trade period. The first translocations were restricted to Alaska, but beginning in 1969 and continuing through 1972, the effort expanded beyond Alaska. During this period, 241 sea otters were translocated to sites in British Columbia, Washington, and Oregon (Jameson et al. 1982). The work was done cooperatively between state and provincial conservation agencies, with much of the financial support for the Oregon and Washington efforts coming from the Atomic Energy Commission (now ERDA). Followup studies of the Oregon population began in 1971 and continued through 1975. After 1975, surveys in Oregon occurred infrequently. In Washington no follow-up surveys were conducted until 1977, although the population has been monitored closely since then (Jameson et al. 1982, 1986; Jeffries and Jameson 1995).

Temporal Trends and Treatment Outcomes of Flexible Ureteroscopy for Lower Pole Stones in a Tertiary Referral Stone Center.

PubMed

Orywal, Ann Kathrin; Knipper, Ann Sophie; Tiburtius, Christian; Gross, Andreas J; Netsch, Christopher

2015-12-01

To evaluate trends in efficacy and safety of flexible ureteroscopy (fURS) for the treatment of lower pole stones (LPS) over a 6-year period and to compare the annual outcomes after one fURS procedure retrospectively. Four hundred nine patients were treated for pure LPS. An analysis of the annual outcomes regarding patient characteristics, operative parameters, stone-free rates (SFRs), and complication rates (CRs) was performed. The SFR was stratified according to stone size and number of stones per patient. Patient data are expressed as mean±standard deviation. There were no annual differences regarding age, body-mass index, stone size (7.09±4.31 mm), number of stones per patient (1.66±2.86), operative time (52.93±33.58 minutes), use of postoperative stents (59.2%), and the Ho:YAG laser lithotripsy rate (49.4%) during the 6-year period. Total primary SFR (annual range) of 97.6% (88.2%-100%), 89.1% (82.9%-96.3%), 71% (60%-78.6%) for urinary calculi <5 mm (3.43±0.78 mm), 5-9 mm (6.69±1.34 mm), and ≥10 mm (13.09±6.02 mm) could be demonstrated over the 6-year period. The total SFR differed significantly between urinary calculi <5 mm, 5-9 mm, and those ≥10 mm (p<0.0179). The SFR (annual range) decreased from 90% (86.2%-95.4%) in patients with 1 stone to 84.1% (60%-100%) in those with ≥3 stones. Perioperative complications (annual range) occurred in 9.7% (6%-13.6%) of the patients (Clavien I 5.1% [1.2%-8.6%], Clavien II 2% [0%-4.9%], Clavien IIIa 0.9% [0%-3.4%], Clavien IIIb 1.7% [1.2%-4.8%]) without differences in the annual CR. fURS is a safe and efficacious procedure for the treatment of LPS with high primary SFR and low CR. fURS in larger LPS (≥10 mm) is associated with the risk for staged procedures.

All Shook Up

ERIC Educational Resources Information Center

Barnes, Janet Bratton

2011-01-01

In this article, the author describes how second-grade students created animal rattles. For finishing touches, students can add faux fur, cords, beads, feathers, and other objects with glue or hot glue. (Contains 1 online resource.)

29 CFR 780.154 - Delivery “to market.”

Code of Federal Regulations, 2013 CFR

2013-07-01

... * * * to market” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, or poultry to market. It ordinarily refers to the...

29 CFR 780.154 - Delivery “to market.”

Code of Federal Regulations, 2014 CFR

2014-07-01

... * * * to market” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, or poultry to market. It ordinarily refers to the...

29 CFR 780.154 - Delivery “to market.”

Code of Federal Regulations, 2012 CFR

2012-07-01

... * * * to market” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, or poultry to market. It ordinarily refers to the...

77 FR 268 - Marine Mammals; File No. 16998

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-04

... of 13,000 Steller sea lions, 200 harbor seals (Phoca vitulina), 10 killer whales (Orcinus orca), 10 humpback whales (Megaptera novaeangliae), and 10 northern fur seals (Callorhinus ursinus). The permit is...

Space Radar Image of Mammoth, California

NASA Technical Reports Server (NTRS)

1999-01-01

This image is a false-color composite of the Mammoth Mountain area in the Sierra Nevada Mountains, California. The image is centered at 37.6 degrees north latitude and 119.0 degrees west longitude. The area is approximately 11.5 kilometers by 78.3 kilometers (7.2 by 48.7 miles) in size. The image was acquired by the Spaceborne Imaging Radar-C and X-Synthetic Aperture Radar (SIR-C/X-SAR) aboard space shuttle Endeavour on its 40th orbit, April 11, 1994. The city of Mammoth Lakes is visible in the bottom right portion of the scene. In this color representation, red is C-band HV-polarization, green is C-band VV-polarization and blue is the ratio of C-band VV to C-band HV. Blue areas are lakes or slopes facing away from the radar illumination. Yellow represents areas of dry, old snow as well as slopes facing directly the radar illumination. At the time of the SIR-C overflight, the sky conditions were partially cloudy, with low and cold air temperatures. Total snow depth is about 1 to 1.5 meters (3 to 5 feet). The current snow accumulation is only about 40 percent of the average for the season. The most recent snowfall in the area covered the entire area with about 30 centimeters (14 inches) of fresh dry snow. Above 3,000 meters (10,000 feet) elevation the snowpack is dry. Below that elevation, the snowpack has a layered structure. Snow hydrologists are using SIR-C/X-SAR data to determine both the quantity of water held by seasonal snowpack and the amount of snow melting. SIR-C/X-SAR radars illuminate Earth with microwaves allowing detailed observations at any time, regardless of weather or sunlight conditions. SIR-C/X-SAR uses three microwave wavelengths: L-band (24 cm), C-band (6 cm)and X-band (3 cm). The multi-frequency data will be used by the international scientific community to better understand the global environment and how it is changing. The SIR-C/X-SAR data, in conjunction with aircraft and ground studies, will give scientists clearer insights into those environmental changes which are caused by nature and those changes which are induced by human activity. SIR-C was developed by NASA's Jet Propulsion Laboratory. X-SAR was developed by the Dornier and Alenia Spazio companies for the German space agency, Deutsche Agentur fuer Raumfahrtangelegenheiten (DARA), and the Italian space agency, Agenzia Spaziale Italiana (ASI) with the Deutsche Forschungsanstalt fur Luft und Raumfahrt e.v. (DLR), the major partner in science, operation and data processing of X-SAR.

Air traffic simulation in chemistry-climate model EMAC 2.41: AirTraf 1.0

NASA Astrophysics Data System (ADS)

Yamashita, Hiroshi; Grewe, Volker; Jöckel, Patrick; Linke, Florian; Schaefer, Martin; Sasaki, Daisuke

2016-09-01

Mobility is becoming more and more important to society and hence air transportation is expected to grow further over the next decades. Reducing anthropogenic climate impact from aviation emissions and building a climate-friendly air transportation system are required for a sustainable development of commercial aviation. A climate optimized routing, which avoids climate-sensitive regions by re-routing horizontally and vertically, is an important measure for climate impact reduction. The idea includes a number of different routing strategies (routing options) and shows a great potential for the reduction. To evaluate this, the impact of not only CO2 but also non-CO2 emissions must be considered. CO2 is a long-lived gas, while non-CO2 emissions are short-lived and are inhomogeneously distributed. This study introduces AirTraf (version 1.0) that performs global air traffic simulations, including effects of local weather conditions on the emissions. AirTraf was developed as a new submodel of the ECHAM5/MESSy Atmospheric Chemistry (EMAC) model. Air traffic information comprises Eurocontrol's Base of Aircraft Data (BADA Revision 3.9) and International Civil Aviation Organization (ICAO) engine performance data. Fuel use and emissions are calculated by the total energy model based on the BADA methodology and Deutsches Zentrum für Luft- und Raumfahrt (DLR) fuel flow method. The flight trajectory optimization is performed by a genetic algorithm (GA) with respect to a selected routing option. In the model development phase, benchmark tests were performed for the great circle and flight time routing options. The first test showed that the great circle calculations were accurate to -0.004 %, compared to those calculated by the Movable Type script. The second test showed that the optimal solution found by the algorithm sufficiently converged to the theoretical true-optimal solution. The difference in flight time between the two solutions is less than 0.01 %. The dependence of the optimal solutions on the initial set of solutions (called population) was analyzed and the influence was small (around 0.01 %). The trade-off between the accuracy of GA optimizations and computational costs is clarified and the appropriate population and generation (one iteration of GA) sizing is discussed. The results showed that a large reduction in the number of function evaluations of around 90 % can be achieved with only a small decrease in the accuracy of less than 0.1 %. Finally, AirTraf simulations are demonstrated with the great circle and the flight time routing options for a typical winter day. The 103 trans-Atlantic flight plans were used, assuming an Airbus A330-301 aircraft. The results confirmed that AirTraf simulates the air traffic properly for the two routing options. In addition, the GA successfully found the time-optimal flight trajectories for the 103 airport pairs, taking local weather conditions into account. The consistency check for the AirTraf simulations confirmed that calculated flight time, fuel consumption, NOx emission index and aircraft weights show good agreement with reference data.

Seasonal effects on the nucleus of comet 67P revealed by Rosetta/VIRTIS

NASA Astrophysics Data System (ADS)

Tosi, Federico; Capaccioni, Fabrizio; Filacchione, Gianrico; Erard, Stéphane; Rouseeau, Batiste; Combe, Jean-Philippe; Capria, Maria Teresa; Leyrat, Cédric; Longobardo, Andrea; Bockelée-Morvan, Dominique; Kappel, David; Arnold, Gabriele; Fonti, Sergio; Mancarella, Francesca; Kuehrt, Ekkehard; Mottola, Stefano

2016-04-01

We describe thermal effects on the nucleus of comet 67P. Due to the overall low thermal inertia of the nucleus surface, the surface temperature is essentially dominated by the instantaneous value of the solar incidence angle and the heliocentric distance. However, for each location, the smallest achievable value of insolation angle depends on the season and topography. Given the substantial obliquity of comet 67P, seasons are such that the northern hemisphere is mainly illuminated at aphelion while the southern hemisphere receives most insolation soon after perihelion. In addition, the heliocentric distance strongly affects the surface temperature, all other parameters being equal. This is a larger effect in comets than in asteroids, due to the wide range of heliocentric distance values spanned by comets. When Rosetta started its global mapping observation campaign, in early August 2014, hyperspectral images acquired by the VIRTIS imaging spectrometer onboard the Rosetta Orbiter covered only the northern regions of the cometary surface, and the equatorial belt became gradually unveiled, while the southern region has been revealed from 2015 onwards. In parallel, the comet's heliocentric distance has been decreasing from ˜3.6 AU down to 1.24 AU, the distance at which the perihelion passage occurred on 13 August 2015. By relating surface temperatures as measured by VIRTIS to three variables: solar incidence angle, true local solar time and heliocentric distance, we aim to separate the relative contributions due to season and to the heliocentric distance. To do this, we use both VIRTIS-M data (namely data from the mapping spectrometer covering the 1-5 μm range, available up to April 2015, i.e. before the failure of the IR cryocooler) and VIRTIS-H data (namely data from the high-resolution point spectrometer covering the 2-5 μm range), and we focus in particular on three regions: one in the northern hemisphere, one in the equatorial region and one in the southern hemisphere. These three regions are chosen so as to be relatively smooth at the spatial resolution that is achieved from a distance of about 100 km (25 m/px for VIRTIS-M, 50×150 m/px for VIRTIS-H), in order to limit the effects of large-scale surface roughness. Acknowledgements: The authors would like to thank the following institutions and agencies, which supported this work: Italian Space Agency (ASI - Italy), Centre National d'Etudes Spatiales (CNES- France), Deutsches Zentrum für Luft- und Raumfahrt (DLR-Germany), National Aeronautic and Space Administration (NASA-USA) Rosetta Program, Science and Technology Facilities Council (UK). VIRTIS has been built by a consortium, which includes Italy, France and Germany, under the scientific responsibility of the Istituto di Astrofisica e Planetologia Spaziali of INAF, Italy, which guides also the scientific operations. The VIRTIS instrument development has been funded and managed by ASI, with contributions from Observatoire de Meudon financed by CNES, and from DLR. The computational resources used in this research have been supplied by INAF-IAPS through the DataWell project.

76 FR 68719 - Marine Mammals; File No. 14676

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-07

... seals (Phoca vitulina), 1,000 northern elephant seals (Mirounga angustirostris), and 150 northern fur... year, for a total of 15 per year. The amendment would be valid through the expiration date of the...

9 CFR 149.3 - Site audit.

Code of Federal Regulations, 2011 CFR

2011-01-01

... consists of wildlife feces, footprints, fur, or hair observed in or near the stored feed or feed.... The sterile zone must be devoid of any harborage or feed or water sources that could attract rodents...

77 FR 34352 - Marine Mammals; File No. 17178

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-11

... permit has been issued to Virginia Institute of Marine Science [Responsible Party: Elizabeth Canuel, Ph.D... import of fur, blood, and fat biopsies from up to 300 crabeater seals (Lobodon carcinophaga), 200 Weddell...

Technique Selectively Represses Immune System

MedlinePlus

... from attacking myelin in a mouse model of multiple sclerosis. Dr David Furness, Wellcome Images. All rights reserved ... devised a way to successfully treat symptoms resembling multiple sclerosis in a mouse model. With further development, the ...

29 CFR 780.153 - Delivery “to storage.”

Code of Federal Regulations, 2010 CFR

2010-07-01

... storage” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, or poultry to the places where they are to be stored or...

Pet Allergy

MedlinePlus

... chronic) airway inflammation associated with asthma. Cats and dogs Allergens from cats and dogs are found in skin cells the animals shed ( ... can become airborne. So-called hypoallergenic cats and dogs may shed less fur than shedding types, but ...

A Tandetron as proton injector for the eye tumor therapy in Berlin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Roehrich, J.; Damerow, T.; Hahn, W.

2012-02-15

The therapy of eye tumors with fast protons is an excellent tool giving very high local control rates. At the Helmholtz-Zentrum Berlin (HZB) almost 1800 patients were treated since 1998. A 2 MV Tandetron was installed as injector for the k = 132 HZB cyclotron. Using the standard 358 duoplasmatron ion source with direct extraction of negative hydrogen ions an extremely stable proton beam can be delivered, both on the short-term and the long-term scale. The hair-needle filaments made from thoriated tungsten wires have safe operation times of more than 1000 h.

High-flux PGAA for milligram-weight samples

NASA Astrophysics Data System (ADS)

Kudejova, P.; Révay, Z.; Kleszcz, K.; Genreith, C.; Rossbach, M.

2015-05-01

With the high-intensity cold neutron flux available at the Prompt Gamma Activation Analysis (PGAA) instrument of the research reactor FRM II at the Heinz Maier-Leibnitz Zentrum (MLZ), samples with a weight of 1 mg or even less can be investigated for their elemental compositions using the (n,γ) capture reaction. In such cases, the typical sample packing material for PGAA experiments made of 25 μm thick PTFE foil (ca. 80 mg) can be orders of magnitude more massive than the sample weight itself. Proper choice of the packing material and measuring conditions are then of the highest importance [1].

Correction of mouse ornithine transcarbamylase deficiency by gene transfer into the germ line.

PubMed Central

Cavard, C; Grimber, G; Dubois, N; Chasse, J F; Bennoun, M; Minet-Thuriaux, M; Kamoun, P; Briand, P

1988-01-01

The sparse fur with abnormal skin and hair (Spf-ash) mouse is a model for the human X-linked hereditary disorder, ornithine transcarbamylase (OTC) deficiency. In Spf-ash mice, both OTC mRNA and enzyme activity are 5% of control values resulting in hyperammonemia, pronounced orotic aciduria and an abnormal phenotype characterized by growth retardation and sparse fur. Using microinjection, we introduced a construction containing rat OTC cDNA linked to the SV40 early promoter into fertilized eggs of Spf-ash mice. The expression of the transgene resulted in the development of a transgenic mouse whose phenotype and orotic acid excretion are fully normalized. Thus, the possibility of correcting hereditary enzymatic defect by gene transfer of heterologous cDNA coding for the normal enzyme has been demonstrated. Images PMID:3162766

Evaluation of pharmaceuticals in surface water: reliability of PECs compared to MECs.

PubMed

Celle-Jeanton, Hélène; Schemberg, Dimitri; Mohammed, Nabaz; Huneau, Frédéric; Bertrand, Guillaume; Lavastre, Véronique; Le Coustumer, Philippe

2014-12-01

Due to the current analytical processes that are not able to measure all the pharmaceutical molecules and to the high costs and the consumption of time to sample and analyze PhACs, models to calculate Predicted Environmental Concentrations (PECs) have been developed. However a comparison between MECs and PECs, taking into account the methods of calculations and peculiarly the parameters included in the calculation (consumption data, pharmacokinetic parameters, elimination rate in STPs and in the environment), is necessary to assess the validity of PECs. MEC variations of sixteen target PhACs [acetaminophen (ACE), amlodipine (AML), atenolol (ATE), caffeine (CAF), carbamazepine (CAR), doxycycline (DOX), epoxycarbamazepine (EPO), fluvoxamine (FLU), furosemide (FUR), hydrochlorothiazide (HYD), ifosfamide (IFO), losartan (LOS), pravastatin (PRA), progesterone (PROG), ramipril (RAM), trimetazidine (TRI)] have been evaluated during one hydrological cycle, from October 2011 to October 2012 and compared to PECs calculated by using an adaptation of the models proposed by Heberer and Feldmann (2005) and EMEA (2006). Comparison of PECs and MECS has been achieved for six molecules: ATE, CAR, DOX, FUR, HYD and PRA. DOX, FUR and HYD present differences between PECs and MECs on an annual basis but their temporal evolutions follow the same trends. PEC evaluation for these PhACs could then be possible but need some adjustments of consumption patterns, pharmacokinetic parameters and/or mechanisms of (bio)degradation. ATE, CAR and PRA are well modeled; PECs can then be used as reliable estimation of concentrations without any reserve. Copyright © 2014 Elsevier Ltd. All rights reserved.

Drinking behaviour and water turnover rates of Antarctic fur seal pups: implications for the estimation of milk intake by isotopic dilution.

PubMed

Lea, Mary-Anne; Bonadonna, Francesco; Hindell, Mark A; Guinet, Christophe; Goldsworthy, Simon D

2002-06-01

The estimation of milk consumption in free-ranging seals using tritium dilution techniques makes the key assumption that the animals drink no pre-formed water during the experimental period. However, frequent observations of unweaned Antarctic fur seal pups drinking water at Iles Kerguelen necessitated the testing of this assumption. We estimated water flux rates of 30 pups (10.7+/-0.3 kg) in four experimental groups by isotopic dilution over 4 days. The groups were: (1) pups held in an open air enclosure without access to water to estimate fasting metabolic water production (MWP); (2) free-ranging pups not administered additional water; (3) pups held in an open air enclosure and given a total of 300 ml of fresh water to verify technique accuracy; and (4) free-ranging pups given 200 ml of fresh water. Pups without access to water exhibited water flux rates (20.5+/-0.8 ml kg(-1)d(-1)), which were significantly lower than those observed for the free-ranging group (33.0+/-1.7 ml kg(-1) d(-1)). Mean estimated pre-formed water intake for the free-ranging experimental groups was 12.6 ml kg(-1) d(-1). Thus, MWP, measured as total water intake during fasting, may be significantly over-estimated in free-ranging Antarctic fur seal pups at Iles Kerguelen and at other sites and subsequently milk intake rates may be underestimated.

A redescription of Lithornis vulturinus (Aves, Palaeognathae) from the Early Eocene Fur Formation of Denmark.

PubMed

Bourdon, Estelle; Lindow, Bent

2015-10-20

The extinct Lithornithidae include several genera and species of flying palaeognathous birds of controversial affinities known from the Early Paleogene of North America and Europe. An almost complete, articulated skeleton from the Early Eocene marine deposits of the Fur Formation (Denmark) was recently assigned to Lithornis vulturinus Owen, 1840. This study provides a detailed redescription and comparison of this three-dimensionally preserved specimen (MGUH 26770), which is one of the best preserved representatives of the Lithornithidae yet known. We suggest that some new features might be diagnostic of Lithornis vulturinus, including a pterygoid fossa shallower than in other species of Lithornis and the presence of a small caudal process on the os palatinum. We propose that Lithornis nasi (Harrison, 1984) is a junior synonym of Lithornis vulturinus and we interpret minor differences in size and shape among the specimens as intraspecific variation. To date, Lithornis vulturinus is known with certainty from the latest Paleocene-earliest Eocene to Early Eocene of the North Sea Basin (Ølst, Fur and London Clay Formations). Among the four species of the genus Lithornis, the possibility that Lithornis plebius Houde, 1988 (Early Eocene of Wyoming) is conspecific with either Lithornis vulturinus or Lithornis promiscuus Houde, 1988 (Early Eocene of Wyoming) is discussed. The presence of closely related species of Lithornis on either side of the North Atlantic in the Early Eocene reflects the existence of a high-latitude land connection between Europe and North America at that time.

Winter habitat predictions of a key Southern Ocean predator, the Antarctic fur seal (Arctocephalus gazella)

NASA Astrophysics Data System (ADS)

Arthur, Benjamin; Hindell, Mark; Bester, Marthan; De Bruyn, P. J. Nico; Trathan, Phil; Goebel, Michael; Lea, Mary-Anne

2017-06-01

Quantification of the physical and biological environmental factors that influence the spatial distribution of higher trophic species is central to inform management and develop ecosystem models, particularly in light of ocean changes. We used tracking data from 184 female Antarctic fur seals (Arctocephalus gazella) to develop habitat models for three breeding colonies for the poorly studied Southern Ocean winter period. Models were used to identify and predict the broadly important winter foraging habitat and to elucidate the environmental factors influencing these areas. Model predictions closely matched observations and several core areas of foraging habitat were identified for each colony, with notable areas of inter-colony overlap suggesting shared productive foraging grounds. Seals displayed clear choice of foraging habitat, travelling through areas of presumably poorer quality to access habitats that likely offer an energetic advantage in terms of prey intake. The relationships between environmental predictors and foraging habitat varied between colonies, with the principal predictors being wind speed, sea surface temperature, chlorophyll a concentration, bathymetry and distance to the colony. The availability of core foraging areas was not consistent throughout the winter period. The habitat models developed in this study not only reveal the core foraging habitats of Antarctic fur seals from multiple colonies, but can facilitate the hindcasting of historical foraging habitats as well as novel predictions of important habitat for other major colonies currently lacking information of the at-sea distribution of this major Southern Ocean consumer.

The functional requirements of mammalian hair: a compromise between crypsis and thermoregulation?

PubMed

Wacker, Chris B; McAllan, Bronwyn M; Körtner, Gerhard; Geiser, Fritz

2016-08-01

Mammalian fur often shows agouti banding with a proximal dark band near the skin and a lighter distal band. We examined the function of both bands in relation to camouflage, thermal properties of pelts, and thermal energetics of dunnarts (Sminthopsis crassicaudata), which are known to use torpor and basking. Although the distal band of dunnart fur darkened with increasing latitude, which is important for camouflage, it did not affect the thermal properties and the length of the dark band and total hair length were not correlated. In contrast, the length of the proximal dark band of preserved pelts exposed to sunlight was positively correlated (r (2) = 0.59) with the temperature underneath the pelt (T pelt). All dunnarts offered radiant heat basked by exposing the dark band of the hair during both rest and torpor. Basking dunnarts with longer dark bands had lower resting metabolism (r (2) = 0.69), warmed faster from torpor (r (2) = 0.77), required less energy to do so (r (2) = 0.32), and reached a higher subcutaneous temperature (T sub) at the end of rewarming (r (2) = 0.75). We provide the first experimental evidence on the possible dual function of the color banding of mammalian fur. The distal colored band appears to be important for camouflage, whereas the length of the dark proximal hair band facilitates heat gain for energy conservation and allows animals to rewarm quickly and economically from torpor.

WW domains of Rsp5p define different functions: determination of roles in fluid phase and uracil permease endocytosis in Saccharomyces cerevisiae.

PubMed

Gajewska, B; Kamińska, J; Jesionowska, A; Martin, N C; Hopper, A K; Zoładek, T

2001-01-01

Rsp5p, ubiquitin-protein ligase, an enzyme of the ubiquitination pathway, contains three WW domains that mediate protein-protein interactions. To determine if these domains adapt Rsp5p to a subset of substrates involved in numerous cellular processes, we generated mutations in individual or combinations of the WW domains. The rsp5-w1, rsp5-w2, and rsp5-w3 mutant alleles complement RSP5 deletions at 30 degrees. Thus, individual WW domains are not essential. Each rsp5-w mutation caused temperature-sensitive growth. Among variants with mutations in multiple WW domains, only rsp5-w1w2 complemented the deletion. Thus, the WW3 domain is sufficient for Rsp5p essential functions. To determine whether rsp5-w mutations affect endocytosis, fluid phase and uracil permease (Fur4p) endocytosis was examined. The WW3 domain is important for both processes. WW2 appears not to be important for fluid phase endocytosis whereas it is important for Fur4p endocytosis. In contrast, the WW1 domain affects fluid phase endocytosis, but it does not appear to function in Fur4p endocytosis. Thus, various WW domains play different roles in the endocytosis of these two substrates. Rsp5p is located in the cytoplasm in a punctate pattern that does not change during the cell cycle. Altering WW domains does not change the location of Rsp5p.

WW domains of Rsp5p define different functions: determination of roles in fluid phase and uracil permease endocytosis in Saccharomyces cerevisiae.

PubMed Central

Gajewska, B; Kamińska, J; Jesionowska, A; Martin, N C; Hopper, A K; Zoładek, T

2001-01-01

Rsp5p, ubiquitin-protein ligase, an enzyme of the ubiquitination pathway, contains three WW domains that mediate protein-protein interactions. To determine if these domains adapt Rsp5p to a subset of substrates involved in numerous cellular processes, we generated mutations in individual or combinations of the WW domains. The rsp5-w1, rsp5-w2, and rsp5-w3 mutant alleles complement RSP5 deletions at 30 degrees. Thus, individual WW domains are not essential. Each rsp5-w mutation caused temperature-sensitive growth. Among variants with mutations in multiple WW domains, only rsp5-w1w2 complemented the deletion. Thus, the WW3 domain is sufficient for Rsp5p essential functions. To determine whether rsp5-w mutations affect endocytosis, fluid phase and uracil permease (Fur4p) endocytosis was examined. The WW3 domain is important for both processes. WW2 appears not to be important for fluid phase endocytosis whereas it is important for Fur4p endocytosis. In contrast, the WW1 domain affects fluid phase endocytosis, but it does not appear to function in Fur4p endocytosis. Thus, various WW domains play different roles in the endocytosis of these two substrates. Rsp5p is located in the cytoplasm in a punctate pattern that does not change during the cell cycle. Altering WW domains does not change the location of Rsp5p. PMID:11139494

Organohalogen contaminants and vitamins in Northern Fur Seals (Callorhinus ursinus) collected during subsistence hunts in Alaska

PubMed Central

Reiner, Jessica L.; Becker, Paul R.; Gribble, Matthew O.; Lynch, Jennifer M.; Moors, Amanda J.; Ness, Jennifer; Peterson, Danielle; Pugh, Rebecca S.; Ragland, Tamika; Rimmer, Catherine; Rhoderick, Jody; Schantz, Michele M.; Trevillian, Jennifer; Kucklick, John R.

2016-01-01

During native subsistence hunts from 1987 to 2007, blubber and liver samples from 50 subadult male northern fur seals (Callorhinus ursinus) were collected on St. Paul Island, Alaska. Samples were analyzed for legacy persistent organic pollutants (POPs), recently phased out/current-use POPs, and vitamins. The legacy POPs measured from blubber samples included polychlorinated biphenyl congeners, dichlorodiphenyltrichloroethanes (DDT and metabolites), chlorobenzenes, chlordanes, and mirex. Recently phased-out/current-use POPs included in the blubber analysis were the flame retardants, polybrominated diphenyl ethers, and hexabromocyclododecanes. The chemical surfactants, perfluorinated alkyl acids and vitamins A and E were assessed in the liver samples. Overall, concentrations of legacy POPs are similar to levels seen in seal samples from other areas of the North Pacific Ocean and the Bering Sea. Statistically significant correlations were seen between compounds with similar functions (pesticides, flame retardants, vitamins). With sample collection spanning two decades, the temporal trends in the concentrations of POPs and vitamins were assessed. For these animals, the concentrations of the legacy POPs tend to decrease or stay the same with sampling year; however, the concentrations of the current-use POPs increased with sampling year. Vitamin concentrations tended to stay the same across the sampling years. With the population of northern fur seals from St. Paul Island on the decline, a detailed assessment of exposure to contaminants and the correlations with vitamins fills a critical gap for identifying potential population risk factors that might be associated with health effects. PMID:26142120

The functional requirements of mammalian hair: a compromise between crypsis and thermoregulation?

NASA Astrophysics Data System (ADS)

Wacker, Chris B.; McAllan, Bronwyn M.; Körtner, Gerhard; Geiser, Fritz

2016-08-01

Mammalian fur often shows agouti banding with a proximal dark band near the skin and a lighter distal band. We examined the function of both bands in relation to camouflage, thermal properties of pelts, and thermal energetics of dunnarts ( Sminthopsis crassicaudata), which are known to use torpor and basking. Although the distal band of dunnart fur darkened with increasing latitude, which is important for camouflage, it did not affect the thermal properties and the length of the dark band and total hair length were not correlated. In contrast, the length of the proximal dark band of preserved pelts exposed to sunlight was positively correlated ( r 2 = 0.59) with the temperature underneath the pelt ( T pelt). All dunnarts offered radiant heat basked by exposing the dark band of the hair during both rest and torpor. Basking dunnarts with longer dark bands had lower resting metabolism ( r 2 = 0.69), warmed faster from torpor ( r 2 = 0.77), required less energy to do so ( r 2 = 0.32), and reached a higher subcutaneous temperature ( T sub) at the end of rewarming ( r 2 = 0.75). We provide the first experimental evidence on the possible dual function of the color banding of mammalian fur. The distal colored band appears to be important for camouflage, whereas the length of the dark proximal hair band facilitates heat gain for energy conservation and allows animals to rewarm quickly and economically from torpor.

Expression of multidrug resistance efflux pump gene norA is iron responsive in Staphylococcus aureus.

PubMed

Deng, Xin; Sun, Fei; Ji, Quanjiang; Liang, Haihua; Missiakas, Dominique; Lan, Lefu; He, Chuan

2012-04-01

Staphylococcus aureus utilizes efflux transporter NorA to pump out a wide range of structurally dissimilar drugs, conferring low-level multidrug resistance. The regulation of norA expression has yet to be fully understood although past studies have revealed that this gene is under the control of the global transcriptional regulator MgrA and the two-component system ArlRS. To identify additional regulators of norA, we screened a transposon library in strain Newman expressing the transcriptional fusion norA-lacZ for altered β-galactosidase activity. We identify a transposon insertion in fhuB, a gene that encodes a ferric hydroxamate uptake system permease, and propose that the norA transcription is iron responsive. In agreement with this observation, addition of FeCl(3) repressed the induction of norA-lacZ, suggesting that bacterial iron uptake plays an important role in regulating norA transcription. In addition, a fur (ferric uptake regulator) deletion exhibited compromised norA transcription and reduced resistance to quinolone compared to the wild-type strain, indicating that fur functions as a positive regulator of norA. A putative Fur box identified in the promoter region of norA was confirmed by electrophoretic mobility shift and DNase I footprint assays. Finally, by employing a siderophore secretion assay, we reveal that NorA may contribute to the export of siderophores. Collectively, our experiments uncover some novel interactions between cellular iron level and norA regulation in S. aureus.

Expression of Multidrug Resistance Efflux Pump Gene norA Is Iron Responsive in Staphylococcus aureus

PubMed Central

Deng, Xin; Sun, Fei; Ji, Quanjiang; Liang, Haihua; Missiakas, Dominique; Lan, Lefu

2012-01-01

Staphylococcus aureus utilizes efflux transporter NorA to pump out a wide range of structurally dissimilar drugs, conferring low-level multidrug resistance. The regulation of norA expression has yet to be fully understood although past studies have revealed that this gene is under the control of the global transcriptional regulator MgrA and the two-component system ArlRS. To identify additional regulators of norA, we screened a transposon library in strain Newman expressing the transcriptional fusion norA-lacZ for altered β-galactosidase activity. We identify a transposon insertion in fhuB, a gene that encodes a ferric hydroxamate uptake system permease, and propose that the norA transcription is iron responsive. In agreement with this observation, addition of FeCl3 repressed the induction of norA-lacZ, suggesting that bacterial iron uptake plays an important role in regulating norA transcription. In addition, a fur (ferric uptake regulator) deletion exhibited compromised norA transcription and reduced resistance to quinolone compared to the wild-type strain, indicating that fur functions as a positive regulator of norA. A putative Fur box identified in the promoter region of norA was confirmed by electrophoretic mobility shift and DNase I footprint assays. Finally, by employing a siderophore secretion assay, we reveal that NorA may contribute to the export of siderophores. Collectively, our experiments uncover some novel interactions between cellular iron level and norA regulation in S. aureus. PMID:22267518

Fur-dependent detoxification of organic acids by rpoS mutants during prolonged incubation under aerobic, phosphate starvation conditions.

PubMed

Guillemet, Mélanie L; Moreau, Patrice L

2008-08-01

The activity of amino acid-dependent acid resistance systems allows Escherichia coli to survive during prolonged incubation under phosphate (P(i)) starvation conditions. We show in this work that rpoS-null mutants incubated in the absence of any amino acid survived during prolonged incubation under aerobic, P(i) starvation conditions. Whereas rpoS(+) cells incubated with glutamate excreted high levels of acetate, rpoS mutants grew on acetic acid. The characteristic metabolism of rpoS mutants required the activity of Fur (ferric uptake regulator) in order to decrease the synthesis of the small RNA RyhB that might otherwise inhibit the synthesis of iron-rich proteins. We propose that RpoS (sigma(S)) and the small RNA RyhB contribute to decrease the synthesis of iron-rich proteins required for the activity of the tricarboxylic acid (TCA) cycle, which redirects the metabolic flux toward the production of acetic acid at the onset of stationary phase in rpoS(+) cells. In contrast, Fur activity, which represses ryhB, and the lack of RpoS activity allow a substantial activity of the TCA cycle to continue in stationary phase in rpoS mutants, which decreases the production of acetic acid and, eventually, allows growth on acetic acid and P(i) excreted into the medium. These data may help explain the fact that a high frequency of E. coli rpoS mutants is found in nature.

Trace elements bioaccumulation in liver and fur of Myotis myotis from two caves of the eastern side of Sicily (Italy): A comparison between a control and a polluted area.

PubMed

Ferrante, Margherita; Spena, Maria Teresa; Hernout, Béatrice Veronique; Grasso, Alfina; Messina, Andrea; Grasso, Rosario; Agnelli, Paolo; Brundo, Maria Violetta; Copat, Chiara

2018-05-07

Environmental pollution is a topic of great interest because it directly affects the quality of ecosystems and of all living organisms at different trophic and systematic levels. Together with the global climate change, the long-term surviving of many species of plants and animals is threaten, distributional patterns at global and regional levels are altered and it results in local assemblages of species that are quite different from those that currently constitute coevolved communities. .For this study, the species Myotis myotis was used as bioindicator and it was sampled from two caves in the south-east of Sicily, Pipistrelli chosen as control area and Palombara chosen as polluted area, to measure the concentrations of trace elements in fur and liver tissues. Results showed higher content of essential elements in fur in bats sampled from Pipistrelli. Conversely, higher concentrations of toxic metals in liver such as As, Cd, Pb and Hg were measured in bat samples in Palombara cave, where specimens have a hunting area extended within the boundaries of the petrochemical plant. Nevertheless, we cannot consider Palombara population as polluted by metal contamination since their tissue concentrations are overall lower than toxic thresholds values suggested for small mammals. Likewise, we cannot exclude other kind of pollutants as potential stressors of the examined population, contributing with the decreasing of bat colonies in Sicily. Copyright © 2018 Elsevier Ltd. All rights reserved.

The mouse p (pink-eyed dilution) and human P genes, oculocutaneous albinism type 2 (OCA2), and melanosomal pH.

PubMed

Brilliant, M H

2001-04-01

Recessive mutations of the mouse p (pink-eyed dilution) gene lead to hypopigmentation of the eyes, skin, and fur. Mice lacking a functional p protein have pink eyes and light gray fur (if non-agouti) or cream-colored fur (if agouti). The human orthologue is the P protein. Humans lacking a functional P protein have oculocutaneous albinism type 2 (OCA2). Melanocytes from p-deficient mice or OCA2 individuals contain small, minimally pigmented melanosomes. The mouse and human proteins are predicted to have 12 membrane spanning domains and possess significant sequence homology to a number of membrane transport proteins, some of which are involved in the transport of anions. The p protein has been localized to the melanosome membrane. Recently, it has been shown that melanosomes from p protein-deficient melanocytes have an abnormal pH. Melanosomes in cultured melanocytes derived from wild-type mice are typically acidic, whereas melanosomes from p protein-deficient mice are non-acidic. Melanosomes and related endosome-derived organelles (i.e., lysosomes) are thought to have an adenosine triphosphate (ATP)-driven proton pump that helps to generate an acidic lumen. To compensate for the charge of these protons, anions must also be transported to the lumen of the melanosome. In light of these observations, a model of p protein function is presented in which the p protein, together with the ATP-driven proton pump, regulates the pH of the melanosome.

Notes on Current Planning for Higher Education in the Federal Republic of Germany.

ERIC Educational Resources Information Center

Heister, Matthias M.

1985-01-01

Objectives, methods, and problems of higher education planning agencies are described, and the work of the Bund-Lander-Kommission fur Bildungsplanung und Forschungsforderung is given particular emphasis. (Author/RM)

76 FR 28421 - Marine Mammals; File No. 15646

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-17

... Rebecca Dickhut, Ph.D., Virginia Institute of Marine Science, P.O. Box 1346, Route 1208 Greate Road... following archived samples will be imported from the Swedish Museum of Natural History: fur, blood, and fat...

77 FR 19646 - Marine Mammals; File No. 17178

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-02

... Virginia Institute of Marine Science [Responsible Party: Elizabeth Canuel, Ph.D.], P.O. Box 1346, Route... will be imported from the Swedish Museum of Natural History: fur, blood, and fat biopsies from up to...

29 CFR 780.155 - Delivery “to carriers for transportation to market.”

Code of Federal Regulations, 2011 CFR

2011-07-01

... market” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, and poultry to any carrier (including carriers by truck...

29 CFR 780.155 - Delivery “to carriers for transportation to market.”

Code of Federal Regulations, 2014 CFR

2014-07-01

... market” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, and poultry to any carrier (including carriers by truck...

29 CFR 780.155 - Delivery “to carriers for transportation to market.”

Code of Federal Regulations, 2012 CFR

2012-07-01

... market” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, and poultry to any carrier (including carriers by truck...

29 CFR 780.155 - Delivery “to carriers for transportation to market.”

Code of Federal Regulations, 2010 CFR

2010-07-01

... market” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, and poultry to any carrier (including carriers by truck...

29 CFR 780.155 - Delivery “to carriers for transportation to market.”

Code of Federal Regulations, 2013 CFR

2013-07-01

... market” includes taking agricultural or horticultural commodities, dairy products, livestock, bees or their honey, fur-bearing animals or their pelts, and poultry to any carrier (including carriers by truck...

Patterns in prey use among fur seals and seabirds in the Pribilof Islands

NASA Astrophysics Data System (ADS)

Sinclair, E. H.; Vlietstra, L. S.; Johnson, D. S.; Zeppelin, T. K.; Byrd, G. V.; Springer, A. M.; Ream, R. R.; Hunt, G. L., Jr.

2008-08-01

We explored correlation in diet trends for five piscivorous predators that reproduce on the Pribilof Islands as illustrative of the shifting structure of the Bering Sea ecosystem. We evaluated the size and species of prey consumed by adult female and juvenile northern fur seals ( Callorhinus ursinus) and adults and chicks of black-legged kittiwakes ( Rissa tridactyla), red-legged kittiwakes ( Rissa brevirostris), thick-billed murres ( Uria lomvia), and common murres ( Uria aalge) from data collected between July and October 1960-2000. Sample sources included stomachs from seals and seabirds collected on pelagic foraging grounds in the eastern Bering Sea, seal scats from rookeries and seabird regurgitations and whole prey from nest sites on St. Paul and St. George Islands of the Pribilof Island archipelago. Typical prey included small fish and invertebrates (⩽20 cm for seals and ⩽12 cm for seabirds) that concentrate along frontal boundaries of the continental shelf/slope and in the epi-pelagic zone. Squids and fishes including walleye pollock ( Theragra chalcogramma), capelin ( Mallotus villosus), and sand lance ( Ammodytes hexapterus) were variably important in the diet of all five predators. Some prey, such as capelin, were principal in predator diets during the 1960s (seals) and into the early 1980s (seabirds), but declined or disappeared from all predator diets thereafter while others, such as walleye pollock, occurred with increasing frequency from the 1970s forward. As the number of individuals consuming walleye pollock increased, the overall volume of pollock in seabird diets declined. This decline was coincident with a decrease in the age and body size of pollock consumed by both seabirds and fur seals. Squid and pollock were negatively correlated in the diets of their primary consumers, northern fur seals (Pearson's coefficient -0.71, p=0.016) and thick-billed murres (Pearson's coefficient=-0.74, p=0.015) from the 1970s forward. Inter-island variation in diet was evident to varying degrees for all predators, with a prevalence of fish on St. Paul Island and invertebrates on St. George Island. Bayesian time-series analysis of synthesized data described significant temporal cross-correlation in diet among northern fur seals, red- and black-legged kittiwakes, and thick-billed murres. For all correlated predators except common murres, beta-binomial modeling indicated that trends in the occurrence of four of the five primary prey (sand lance, capelin, squid, and pollock) evaluated, were significantly associated with eastern Bering Sea time-series trends in sea surface temperature, ice retreat or a combination of both. Data synthesis highlighted potential competition and a scenario for the effects of an altered prey field on the population stability of predators. The association between correlated diet changes among predators and indices of oceanographic shifts in the 1970s and the 1990s allow scrutiny of hypotheses concerning causal mechanisms in population declines.

Anwendungsorientierte Funktionswerkstoffe mittels Walzplattieren

NASA Astrophysics Data System (ADS)

Reichelt, Stephan; Schmidt, J.-F.; Neubauer, M.; Schade, A.; Andler, G.; Buerkle, G.; Hansen, H.; Hofmann, L.; Stiehler, J.; Janisch, S. D.

Obwohl walzplattierte Werkstoffe in der allgemeinen Öffentlichkeit nur wenig beachtet und behandelt werden, besitzen sie bereits heute eine hohe Bedeutung, um unterschiedliche Güter des täglichen Bedarfs realisieren und produzieren zu können. Durch eine hohe Produktivität und ein umfangreiches technologisches Wissen lassen sich bereits heute sehr anspruchsvolle und aufwändige Funktionswerkstoffe mittels Plattierverfahren herstellen, die maßgeschneiderte Produkteigenschaften für viele Halbzeuge und Fertigprodukte garantieren. Bedingt durch die konstruktiven Trends im Bereich von Automotive, Luft- und Raumfahrt sowie dem allgemeinen konstruktiven und materiellem Leichtbau ist davon auszugehen, dass die Bedeutung maßgeschneiderter Funktionswerkstoffe für spezifische Anwendungsfelder in naher Zukunft weiter wachsen und die Bedeutung einzelner Plattierverfahren für eine wirtschaftliche Produktionskette verschiedener Produkte wachsen wird.

16 CFR 301.7 - Describing furs by certain breed names prohibited.

Code of Federal Regulations, 2010 CFR

2010-01-01

..., species, strain or coloring, irrespective of former usage, such descriptive matter shall not contain the... a name in an adjective form or otherwise which connotes a false geographic origin of the animal. For...

16 CFR 301.7 - Describing furs by certain breed names prohibited.

Code of Federal Regulations, 2014 CFR

2014-01-01

..., species, strain or coloring, irrespective of former usage, such descriptive matter shall not contain the... a name in an adjective form or otherwise which connotes a false geographic origin of the animal. For...

16 CFR 301.7 - Describing furs by certain breed names prohibited.

Code of Federal Regulations, 2013 CFR

2013-01-01

..., species, strain or coloring, irrespective of former usage, such descriptive matter shall not contain the... a name in an adjective form or otherwise which connotes a false geographic origin of the animal. For...

16 CFR 301.7 - Describing furs by certain breed names prohibited.

Code of Federal Regulations, 2011 CFR

2011-01-01

..., species, strain or coloring, irrespective of former usage, such descriptive matter shall not contain the... a name in an adjective form or otherwise which connotes a false geographic origin of the animal. For...

16 CFR 301.7 - Describing furs by certain breed names prohibited.

Code of Federal Regulations, 2012 CFR

2012-01-01

..., species, strain or coloring, irrespective of former usage, such descriptive matter shall not contain the... a name in an adjective form or otherwise which connotes a false geographic origin of the animal. For...

Air

MedlinePlus

... cause outdoor air pollution! Indoor pollutants Outdoor pollutants Animal dander (skin and fur) Ground-level ozone (smog) Dust mites Dust* Cockroaches Dirt* Mold Smoke* Secondhand smoke Liquids* Pesticides (either tracked in from outside or used in the house) Industrial emissions (like smoke and ...

16 CFR 301.44 - Misrepresentation of prices.

Code of Federal Regulations, 2010 CFR

2010-01-01

... “bankrupt stock”, “samples”, “show room models”, “Hollywood Models”, “Paris Models”, “French Models”, “Parisian Creations”, “Furs Worn by Society Women”, “Clearance Stock”, “Auction Stock”, “Stock of a business...

PULSED UV: REALITIES OF ENHANCED DISINFECTION

EPA Science Inventory

Quantitative measurements of the light output from low pressure (LP), medium pressure (MP) and the pulsed UV lamps were made using calibrated spectrometry, chemical actinometry and biodosimetry approaches to compare their relative efficiency in producing germicidal UV energy. Fur...