Science.gov

Sample records for zona centro norte

  1. "El Norte," Deracination and Circularity: An Epic Gone Awry

    ERIC Educational Resources Information Center

    Brakel, Arthur

    2007-01-01

    Early journalistic reviews (e.g., Gold, Ebert, and Kael) of "El Norte" (1983), Gregory Nava's first major film, identify it as an epic. In "El Norte" the siblings Enrique and Rosa, two Guatemalan Amerindians, leave their native village on a quest to what for them is the mythical land in the North. Although "El Norte"…

  2. Ichthyofauna of Ceará-Mirim River basin, Rio Grande do Norte State, northeastern Brazil

    PubMed Central

    da Costa, Nathalia Kaluana Rodrigues; de Paiva, Roney Emanuel Costa; da Silva, Márcio Joaquim; Ramos, Telton Pedro Anselmo; Lima, Sergio Maia Queiroz

    2017-01-01

    Abstract Ichthyological studies in coastal basins of the Mid-Northeastern Caatinga ecoregion were first conducted in the early 20th century, including collections from the Ceará-Mirim River basin, in northeastern Brazil. Besides a few systematics and ecological studies, the knowledge on fishes from this watershed is still considered partial and restricted to the freshwater portion. Thus, the objective of this paper was to conduct a comprehensive ichthyological survey of the entire Ceará-Mirim River basin, from the headwaters to the estuarine area. Fish surveys were conducted from 2011 to 2016 using varied fishing gear, resulting in the record of 63 native species (24 freshwater, 15 estuarine, and 24 marine species) and two introduced species. Four species are putatively endemic to the ecoregion, and 48 consist of new records for the basin. According to the Brazilian’s threatened fish list, three species are currently classified as ‘vulnerable’ (Megalops atlanticus, Hippocampus reidi and Mycteroperca bonaci), four as ‘near threatened’ (Kryptolebias hermaphroditus, Dormitator maculatus, Lutjanus sygnagris and L. jocu) and three as ‘data deficient’ (Cheirodon jaguaribensis, Mugil curema and Sphoeroides testudineus). The Ceará-Mirim River basin does not have any protected areas and has been suffering multiple anthropogenic impacts, however the "Centro Tecnológico de Aquicultura" (Aquaculture Technological Center) of the Universidade Federal do Rio Grande do Norte (CTA/UFRN) at the lower portion of the basin may help in the conservation of the estuarine and estuarine fish species. PMID:29302231

  3. Reproductive biology of the Del Norte salamander (Plethodon elongatus).

    Treesearch

    Clara A. Wheeler; Hartwell H. Welsh Jr.; Lisa M. Ollivier

    2013-01-01

    We examined seasonal reproductive patterns of the Del Norte Salamander, Plethodon elongatus, in mixed conifer and hardwood forests of northwestern California and southwestern Oregon. Seasonal size differences in reproductive structures suggested that maximum spermatogenic activity occurred during the late summer, with spermatozoa transfer to the...

  4. 78 FR 30268 - Del Norte County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-22

    ... Schools and Community Self-Determination Act (Pub. L. 112-141) (the Act) and operates in compliance with... to review prior year project's progress. Should the Secure Rural Schools Act be reauthorized, the.... ADDRESSES: The meetings will be held at the Del Norte County Unified School District, Redwood Room, 301 West...

  5. Mechanics of sperm-egg interaction at the zona pellucida.

    PubMed Central

    Baltz, J M; Katz, D F; Cone, R A

    1988-01-01

    Mammalian sperm traverse several layers of egg vestments before fertilization can occur. The innermost vestment, the zona pellucida, is a glycoprotein shell, which captures and tethers the sperm before they penetrate it. We report here direct measurements of the force required to tether a motile human sperm as well as independent calculations of this force using flagellar beat parameters observed for sperm of several species on their homologous zonae. We have compared these sperm-generated forces with the calculated tensile strength of sperm-zona bonds, and found that a motile sperm can be tethered, at least temporarily, by a single bond. Therefore, sperm can be captured by the first bond formed and tethered permanently by a few. The sperm cannot subsequently penetrate the zona unless the bonds are first eliminated. However, premature elimination would simply allow the sperm to escape. Therefore, not only must the bonds be eliminated, but the timing of this must be regulated so that the sperm is already oriented toward the egg and beginning to penetrate as the bonds are broken. Images FIGURE 6 PMID:3224150

  6. Scintigraphy of normal mouse ovaries with monoclonal antibodies to ZP-2, the major zona pellucida protein

    SciTech Connect

    East, I.J.; Keenan, A.M.; Larson, S.M.

    1984-08-31

    The zona pellucida is an extracellular glycocalyx, made of three sulfated glycoproteins, that surrounds mammalian oocytes. Parenterally administered monoclonal antibodies specific for ZP-2, the most abundant zona protein, localize in the zona pellucida. When labeled with iodine-125, these monoclonal antibodies demonstrate a remarkably high target-to-nontarget tissue ratio and provide clear external radioimaging of ovarian tissue.

  7. Calha Norte: Explaining Brazilian Army Presence in the Amazon

    DTIC Science & Technology

    1993-06-01

    the Yanomami Indians.""’ The potential wealth present in the Calha Norte region has been estimated at over US$134 billion, enough to repay almost all...garimpeiros to remain on Yanomami land was essentially a military decision. The Army’s pro-miner position was highlighted by official statements made by the...Environmental Policy and Military Geopolitics in the Development of the Brazilian Amazon: The Case of the Yanomami ," Development and Change (1992

  8. Modeling visibility in the Paso del Norte (PDN) Region

    NASA Astrophysics Data System (ADS)

    Medina Calderon, Richard

    Poor visibility is a subject of growing public concern throughout the U.S, and an active area of research. Its societal impacts on air quality, aviation transport and traffic are significant. Aerosols play a fundamental role in the attenuation of solar radiation, and also affect visibility. The scattering and extinction coefficients of aerosol particles in the Paso del Norte Region have been calculated using the T- matrix model in conjunction with a laser particle counter. Inter-comparison of the model's results of the scattering and absorption coefficients against the corresponding data from a Photoacustic extinctiometer instrument (which measures in-situ absorption and scattering coefficients of aerosol particles) shows excellent agreement. In addition, the volume-weighted method is used to determine the composite index of refraction which is representative of the aerosols for the Paso del Norte Region to obtain information of the type of aerosol particles present in the Region. The Single Scattering Albedo has also been retrieved using this methodology to obtain further insight into the type of aerosols present on a given day. Finally, the Koschmieder equation has been used to calculate the visual range or visibility, and was correlated with the PM2.5 and PM10 particle concentration present in the Region. Our methodology will allow a better understanding of the size and type of aerosol particles that are most detrimental to the visibility for the Paso Del Norte Region.

  9. The practical side of immunocontraception: zona proteins and wildlife.

    PubMed

    Kirkpatrick, J F; Rowan, A; Lamberski, N; Wallace, R; Frank, K; Lyda, R

    2009-12-01

    With shrinking habitat, the humane control of certain wildlife populations is relevant. The contraceptive vaccine based on native porcine zona pellucida (PZP) has been applied to various wildlife populations for 20 years. Prominent efforts include wild horses, urban deer, zoo animals and African elephants, among others. This approach has been successful in managing entire populations and to date, no significant debilitating short- or long-term health effects have been documented.

  10. Transfer of bovine demi-embryos with and without the zona pellucida.

    PubMed

    Warfield, S J; Seidel, G E; Elsden, R P

    1987-09-01

    Bisected bovine embryos with or without the zona pellucida were transferred to recipients nonsurgically in five field trials. Embryos were collected from superovulated donors 6.5 to 7.5 d after estrus; only embryos of good and excellent quality were bisected. Demi-embryos were transferred either within a zona pellucida, without a zona pellucida, without a zona pellucida, or in the third and fourth trials, without a zona but embedded in 7% gelatin. Pregnancies were diagnosed at 44 to 68 d of gestation. In a preliminary trial, 9/29 zona pellucida-intact demi-embryos developed into fetuses compared with 1/10 zona pellucida-free demi-embryos (P greater than .1). The proportion of zona-free demi-embryos developing to fetuses was not significantly different from the zona-intact group in the second trial either, 24/49 and 5/19, respectively. In trial 3, the proportion of zona pellucida-free demi-embryos developing was 8/25; of zona-enclosed embryos, 29/88; and of zona-free demi-embryos embedded in gelatin, 8/22 (P greater than .1). Similarly, in the fourth trial the rate of development of zona-free demi-embryos to fetuses was 5/12, that of zona-enclosed embryos was 32/81, and that of zona-free demi-embryos embedded in gelatin was 3/12 (P greater than .1). In trial 5, survival of zona-enclosed demi-embryos to fetuses was 40/105, and of zona-free demi-embryos, 46/109 (P greater than .1). Except for trial 2, half of the demi-embryos were twinned, one to each uterine horn; twinning did not significantly affect the proportion developing to fetuses for any of the demi-embryo groups. It is concluded that placing post-compaction demi-embryos into the zona pellucida for transfer does not improve pregnancy rates significantly.

  11. 62. R.L.T., photographer November 1, 1934 DEL NORTE COUNTY, SECTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    62. R.L.T., photographer November 1, 1934 DEL NORTE COUNTY, SECTION D, HIGHWAY 1. REDWOOD CLEARING ON EXISTING LINE, 1-DN-71-A #26, R.L.T. 11-1-34. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  12. 63. G.F.H., photographer July 30, 1932 DEL NORTE COUNTY, SECTION ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    63. G.F.H., photographer July 30, 1932 DEL NORTE COUNTY, SECTION A, HIGHWAY 1. 1-DN A #124, STA. 164=00 SHOWING DRAINAGE CONDITIONS, G.F.H., 7-30-32. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  13. Viscous forces are predominant in the zona pellucida mechanical resistance

    NASA Astrophysics Data System (ADS)

    Papi, Massimiliano; Maiorana, Alessandro; Douet, Cécile; Maulucci, Giuseppe; Parasassi, Tiziana; Brunelli, Roberto; Goudet, Ghylène; De Spirito, Marco

    2013-01-01

    The zona pellucida (ZP) is a multilayer glycoprotein spherical shell surrounding mammalian eggs. The ZP's mechanical response plays a crucial role in mammalian fertilization and is a parameter commonly adopted in "in vitro fertilization" to characterize the oocytes quality. While it is assumed that ZP mechanical response is purely elastic, here we prove that dissipative forces cannot be neglected. Physiologically, this evidence implies that an increase in the spermatozoa motility can induce dramatic changes on the ZP reaction force turning ZP shell in an impenetrable barrier leading to fertility impairments.

  14. Classification of Nortes in the Gulf of Mexico derived from wave energy maps

    NASA Astrophysics Data System (ADS)

    Appendini, C. M.; Hernández-Lasheras, J.

    2016-02-01

    Extreme wave climate in the Gulf of Mexico is determined by tropical cyclones and winds from the Central American Cold Surges, locally referred to as Nortes. While hurricanes can have catastrophic effects, extreme waves and storm surge from Nortes occur several times a year, and thus have greater impacts on human activities along the Mexican coast of the Gulf of Mexico. Despite the constant impacts from Nortes, there is no available classification that relates their characteristics (e.g. pressure gradients, wind speed), to the associated coastal impacts. This work presents a first approximation to characterize and classify Nortes, which is based on the assumption that the derived wave energy synthetizes information (i.e. wind intensity, direction and duration) of individual Norte events as they pass through the Gulf of Mexico. First, we developed an index to identify Nortes based on surface pressure differences of two locations. To validate the methodology we compared the events identified with other studies and available Nortes logs. Afterwards, we detected Nortes from the 1986/1987, 2008/2009 and 2009/2010 seasons and used their corresponding wind fields to derive the wave energy maps using a numerical wave model. We used the energy maps to classify the events into groups using manual (visual) and automatic classifications (principal component analysis and k-means). The manual classification identified 3 types of Nortes and the automatic classification identified 5, although 3 of them had a high degree of similarity. The principal component analysis indicated that all events have similar characteristics, as few components are necessary to explain almost all of the variance. The classification from the k-means indicated that 81% of analyzed Nortes affect the southeastern Gulf of Mexico, while a smaller percentage affects the northern Gulf of Mexico and even less affect the western Caribbean.

  15. Ozone studies in the Paso del Norte region

    NASA Astrophysics Data System (ADS)

    Becerra-Davila, Fernando

    The Paso del Norte region forms the largest contiguous bi-national conglomerate on the US-Mexico border. With a combined population of around 2 million inhabitants, the Paso del Norte region is isolated, more than 500 km away from the nearest urban area of comparable size, thus making it an ideal location for air quality studies of an isolated urban environment. The meteorological conditions leading to a high ozone episode in this region, such as the historical ozone episode of June 2006, are analyzed. It is well known that stagnation and minimal winds, high temperatures, and pressure ridges over the region are conducive to high ozone episodes. In addition, the planetary boundary height is studied to understand its impact on high ozone episodes. Several studies report that ground level ozone non-attainment regulations could be caused not only by local emissions, but also by atmospheric transport. In this work the atmospheric advection of pollutants into the region is analyzed using HYSPLIT backward trajectories. Furthermore, a novel backward trajectory clustering technique is implemented for the summer of 2006. The "ozone weekend effect" (OWE) is a phenomenon by which in some geographical regions ambient ozone concentrations tend to be higher on weekends than on weekdays, despite the lower emissions of ozone precursors during those days. The observed local OWE has never previously been studied in terms of the photolysis rates of four of the main ozone precursors. In this research a novel method that allows the calculation of actinic fluxes, photolysis frequencies and photolysis rates with a high degree of accuracy and reliability has been developed. This method utilizes a combination of the experimental data available for this region in conjunction with a radiative transfer model (TUV model). Three weekend-weekday cases during summers 2006, 2009 and 2010 are studied in this work. In this research, the photolysis impact on the local OWE is studied. The results

  16. El Centro College Strategic Plan, 2000-2005.

    ERIC Educational Resources Information Center

    El Centro Coll., Dallas, TX.

    This is the 2000-2005 strategic plan at El Centro College (Texas). It discusses the college's mission, vision, and core values, and provides information on goals and success indicators. Goals include: (1) preparing students for careers and for transfer to four-year institutions; (2) providing quality continuing/workforce education to enrich…

  17. Targeting the zona pellucida for immunocontraception: a minireview.

    PubMed

    Tesarik, J

    1995-12-01

    This minireview summarizes the main data relevant to the development of contraceptive vaccines based on zona pellucida (ZP) antigens, as well as the pros and the cons of this immunocontraceptive strategy. Even though the antifertility efficacy of anti-ZP antibodies in humans is not corroborated by a clear relationship between spontaneous autoimmunization against the ZP and infertility, passive and active immunization studies in laboratory animals have provided convincing results. The contraceptive action of anti-ZP antibodies, targeting events situated upstream of gamete fusion, is devoid of potential ethical concerns related to the destruction of early embryos. The high protein content of the mammalian ZP, knowledge of the complete amino acid sequence of the major ZP proteins, and the high degree of sequence homology between individual species all favour the rapid advancement of anti-ZP vaccine projects. However, certain sequences of ZP proteins, when incorporated into the vaccine construct, activate CD4+ T cells of the recipient organism to direct a cellular immune attack (autoimmune oophoritis) to other functionally relevant ovarian components (primordial follicles, steroidogenic cells). The search for the optimal combination of B cell and T cell epitopes in the vaccine construct will hopefully overcome this problem.

  18. Characterization of oocyte retrieval cycles with empty zona pellucida.

    PubMed

    Oride, Aki; Kanasaki, Haruhiko; Hara, Tomomi; Ohta, Hiroko; Kyo, Satoru

    2018-01-01

    To identify the factors that characterize cycles with empty zona pellucida (EZP). Thirty-six oocyte retrieval cycles from which EZP were collected and another 36 cycles from which no EZP was collected were compared. The patients were divided into three groups: those with no EZP collected during any cycle, those with EZP collected during all cycles, and those experiencing cycles both with and without EZP. The mean number of oocytes collected per cycle was higher in the cycles with EZP than without EZP. The fertilization rate of the collected oocytes and the rate of good embryo formation were significantly lower in the cycles with EZP. No significant difference was observed between the three groups in terms of age, number of oocytes collected, or hormone levels before and after the oocyte retrieval. The fertilization and pregnancy rates were highest in the patients with no EZP being collected during any cycle, followed by those experiencing cycles both with and without EZP, and then by those with EZP collected during all cycles. The observation of lower fertilization, poor embryo formation, and a low pregnancy rate in the patients with EZP suggests the poor quality of oocytes that were collected with EZP in the same cycle.

  19. Microanatomical diversification of the zona pellucida in aplochelioid killifishes.

    PubMed

    Thompson, A W; Furness, A I; Stone, C; Rade, C M; Ortí, G

    2017-07-01

    This study investigates zona pellucida (ZP) ultrastructure in fertilized eggs of annual killifishes (suborder Aplocheiloidei), a group of highly specialized fishes that are able to survive desiccation for several weeks to months before they hatch. Little is known about ZP or chorionic ultrastructure sustaining these life-history modes, so scanning electron microscopy (SEM) was used to describe this trait in a large number of aplocheiloids with a focus on the family Rivulidae and the genus Hypsolebias. New images of ZP ultrastructure for 52 aplocheiloid species are provided, more than doubling the number characterized thus far. The evolution of chorionic structure within this group is studied using these new data. Characters were coded into a morphological matrix and optimized onto a consensus phylogeny to assess phylogenetic signal and reconstruct ancestral character states. Although ZP characters seem highly homoplastic and exhibit a large amount of structural convergence among lineages, aplocheiloid killifishes have evolved a number of unique structures associated with the chorion. Some annual species seem to have lost long filaments because eggs are deposited in the soil instead of being adhered to aquatic plants. © 2017 The Fisheries Society of the British Isles.

  20. Swallowing Quality of Life After Zona Incerta Deep Brain Stimulation.

    PubMed

    Sundstedt, Stina; Nordh, Erik; Linder, Jan; Hedström, Johanna; Finizia, Caterina; Olofsson, Katarina

    2017-02-01

    The management of Parkinson's disease (PD) has been improved, but management of signs like swallowing problems is still challenging. Deep brain stimulation (DBS) alleviates the cardinal motor symptoms and improves quality of life, but its effect on swallowing is not fully explored. The purpose of this study was to examine self-reported swallowing-specific quality of life before and after caudal zona incerta DBS (cZI DBS) in comparison with a control group. Nine PD patients (2 women and 7 men) completed the self-report Swallowing Quality of Life questionnaire (SWAL-QOL) before and 12 months after cZI DBS surgery. The postoperative data were compared to 9 controls. Median ages were 53 years (range, 40-70 years) for patients and 54 years (range, 42-72 years) for controls. No significant differences were found between the pre- or postoperative scores. The SWAL-QOL total scores did not differ significantly between PD patients and controls. The PD patients reported significantly lower scores in the burden subscale and the symptom scale. Patients with PD selected for cZI DBS showed good self-reported swallowing-specific quality of life, in many aspects equal to controls. The cZI DBS did not negatively affect swallowing-specific quality of life in this study.

  1. Zona-free oocyte fertilized with intracytoplasmic sperm injection and underwent further division: case report and literature review.

    PubMed

    Hsieh, Y Y; Chang, C C; Tsai, H D

    2001-09-01

    The zona pellucida (ZP) plays a protective role during fertilization and early embryonic development. It is related to sperm binding, the acrosome reaction, prevention of polyspermic fertilization, and holding blastomeres together before the morular stage. Zona-free oocytes are accidentally encountered. If these oocytes are healthy, they can be fertilized normally by intracytoplasmic sperm injection (ICSI). We reported on a couple with male infertility undergoing oocyte retrieval after ovarian hyperstimulation. Before the ICSI procedure, cumulus cells surrounding the oocytes were removed, which resulted in one oocyte escaping from its ZP. The zona-free oocyte was fertilized normally with ICSI and developed to the 8-cell stage. We observed that the zona-free zygote had the ability to further divide, despite its loose contact. The zona-free embryo was transferred with other zona-intact embryos, but the implantation failed. We conclude that zona-free oocytes can be rescued, fertilized with ICSI, and cultured for further transfer or cryopreservation.

  2. 75 FR 17561 - Amendment to Restricted Area R-2510A; El Centro, CA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-07

    ...-0346; Airspace Docket No. 10-AWP-3] RIN 2120-AA66 Amendment to Restricted Area R-2510A; El Centro, CA... Restricted Area R-2510A, El Centro, CA. Specifically, the action inadvertently omitted the reference to.... 92-AWP-15 entitled ``Alteration and Subdivision of Restricted Area R-2510A; El Centro, CA''. That...

  3. The anatomy of the caudal zona incerta in rodents and primates

    PubMed Central

    Watson, Charles; Lind, Christopher R P; Thomas, Meghan G

    2014-01-01

    The caudal zona incerta is the target of a recent modification of established procedures for deep brain stimulation (DBS) for Parkinson's disease and tremor. The caudal zona incerta contains a number of neuronal populations that are distinct in terms of their cytoarchitecture, connections, and pattern of immunomarkers and is located at a position where a number of major tracts converge before turning toward their final destination in the forebrain. However, it is not clear which of the anatomical features of the region are related to its value as a target for DBS. This paper has tried to identify features that distinguish the caudal zona incerta of rodents (mouse and rat) and primates (marmoset, rhesus monkey, and human) from the remainder of the zona incerta. We studied cytoarchitecture, anatomical relationships, the pattern of immunomarkers, and gene expression in both of these areas. We found that the caudal zona incerta has a number of histological and gene expression characteristics that distinguish it from the other subdivisions of the zona incerta. Of particular note are the sparse population of GABA neurons and the small but distinctive population of calbindin neurons. We hope that a clearer appreciation of the anatomy of the region will in the end assist the interpretation of cases in which DBS is used in human patients. PMID:24138151

  4. Human sperm degradation of zona pellucida proteins contributes to fertilization.

    PubMed

    Saldívar-Hernández, Analilia; González-González, María E; Sánchez-Tusié, Ana; Maldonado-Rosas, Israel; López, Pablo; Treviño, Claudia L; Larrea, Fernando; Chirinos, Mayel

    2015-09-02

    The mammalian oocyte extracellular matrix known as the zona pellucida (ZP) acts as a barrier to accomplish sperm fusion with the female gamete. Although penetration of the ZP is a limiting event to achieve fertilization, this is one of the least comprehended stages of gamete interaction. Even though previous studies suggest that proteases of sperm origin contribute to facilitate the passage of sperm through the ZP, in human this process is not yet fully understood. The aim of this study was to determine the ability of human sperm to degrade recombinant human ZP (rhZPs) proteins and to characterize the proteases involved in this process. Purified rhZP2, rhZP3 and rhZP4 proteins were incubated with capacitated sperm and the proteolytic activity was determined by Western blot analysis. To further characterize the proteases involved, parallel incubations were performed in the presence of the protease inhibitors o-phenanthroline, benzamidine and MG-132 meant to block the activity of metalloproteases, serine proteases and the proteasome, respectively. Additionally, protease inhibitors effect on sperm-ZP binding was evaluated by hemizona assay. The results showed that rhZPs were hydrolyzed in the presence of capacitated sperm. O-phenanthroline inhibited the degradation of rhZP3, MG-132 inhibited the degradation of rhZP4 and benzamidine inhibited the degradation of the three proteins under investigation. Moreover, hemizona assays demonstrated that sperm proteasome inhibition impairs sperm interaction with human native ZP. This study suggests that sperm proteasomes could participate in the degradation of ZP, particularly of the ZP4 protein. Besides, metalloproteases may be involved in specific degradation of ZP3 while serine proteases may contribute to unspecific degradation of the ZP. These findings suggest that localized degradation of ZP proteins by sperm is probably involved in ZP penetration and may be of help in understanding the mechanisms of fertilization in humans.

  5. Effects of porcine zona pellucida immunocontraceptives in zoo felids.

    PubMed

    Harrenstien, Lisa A; Munson, Linda; Chassy, Lisa M; Liu, Irwin K M; Kirkpatrick, Jay F

    2004-09-01

    Methods of contraception are necessary for management of zoo felids; however, the most commonly used contraceptive (melengestrol acetate implant) is associated with serious adverse reactions with long-term use. Porcine zona pellucida (pZP) vaccines are promising as contraceptives, but their safety in zoo felids has not been tested. pZP vaccine was administered to 27 female felids representing 10 species, including African lion (Panthera leo), Asian leopard (P. pardus), jaguar (P. onca), tiger (P. tigris), snow leopard (P. uncia), cougar (Felis concolor), Siberian lynx (F. lynx), Canada lynx (F. canadensis), serval (F. serval), and bobcat (F. rufus), in 15 facilities. Over 6 wk, each animal received three i.m. injections of 65 microg pZP with Freund's complete adjuvant (FCA), Freund's incomplete adjuvant, or carbopol as the adjuvant. Behavioral signs of estrus were seen in 14 of the vaccinated felids. An unacceptably high incidence of adverse reactions was seen including injection site swelling, lameness, limb swelling, or abscessation (or all) in five felids after injection with FCA as the initial adjuvant. Adverse behavioral signs, including increased irritability and aggression, were seen in four felids. Six of the felids were assayed for antibodies against pZP during the 12 mo after vaccination; all showed antibody production. Antibody levels appeared to peak 1-4 mo after vaccination began, although elevated antibody levels persisted in two animals for > 12 mo after the first injection. All vaccinated felids were ovariohysterectomized 3-13 mo after vaccination. Folliculogenesis was present in all treated animals, and there was no histopathologic evidence of inflammatory damage to ovaries. Contraceptive efficacy was not specifically evaluated in this study; however, two of the three felids housed with an intact male became pregnant during the study, one of which gave birth to healthy cubs.

  6. Epidemiological study of scorpion stings in the Rio Grande do Norte State, Northeastern Brazil

    PubMed Central

    de Araújo, Kaliany Adja Medeiros; Tavares, Aluska Vieira; Marques, Michael Radan de Vasconcelos; Vieira, Alecxandro Alves; Leite, Renner de Souza

    2017-01-01

    ABSTRACT This communication is a retrospective epidemiological study of the scorpion sting cases recorded from 2007 to 2014 in the Rio Grande do Norte State, Northeastern Brazil. The data was collected from the Injury Notification Information System database of the Health Department of Rio Grande do Norte State. A total of 20,555 cases were studied. The cases were distributed over all months of the period studied and occurred mainly in urban areas. Victims were predominantly 20-29 year-old women. Most victims were stung on the foot and received medical care within 1-3 h after being stung. The cases were mostly classified as mild and progressed to cure. Scorpion stings in Rio Grande do Norte State are an environmental public health problem that needs to be monitored and controlled throughout the year. PMID:28793026

  7. DELIVERING TIMELY AIR QUALITY, TRAFFIC, AND WEATHER INFORMATION TO YOUR COMMUNITY/THE PASO DEL NORTE ENVIRONMENTAL MONITORING PROJECT

    EPA Science Inventory

    EPA has developed a technology transfer handbook for the EMPACT Paso del Norte Project. The EMPACT Paso del Norte Environmental Monitoring Project is a mobile vehicle emissions project that involves the international community of El Paso, TX; Sundland Park, NM; and Juarez, Mexico...

  8. Solid waste management in Linamon, Lanao del Norte

    NASA Astrophysics Data System (ADS)

    Paragoso, Glaiza P.; Sapar, Cherine Mae A.; Magsayo, Joy R.; Lahoylahoy, Myrna E.; Guarin, Rica Mae B.

    2018-01-01

    In this modern world, Solid Waste Management is very important in maintaining a high quality of life so humans must manage and store waste efficiently and safely. Almost every day each household generates garbage or wastes. People throw things improperly without knowing the consequences. The main objective of the study was to assess the residents' Solid Waste Management. Specifically, it aimed to answer the following questions: What is the profile of the respondents in terms of age, gender, educational attainment, occupation, monthly income, no. of household member; What is the knowledge of the respondents about Natural Environment?; How do respondents disposed garbage from the household?; What is the level of the respondents concern about proper waste management?; What is the willingness of the respondents to participate in proper Solid Waste Management?; What is the attitude of the respondents towards the Solid Waste Management? The said study was conducted at the Municipality of Linamon, which is a 5th class municipality located at the Eastern Gateway to Lanao del Norte, a south western Province of Northern Mindanao in the Philippines. The study was conducted in the 8 barangays of the Municipality of Linamon, namely: Bosque, Larapan, Mago-ong, Napo, Poblacion, Purakan, Robocon, and Samburon. The Municipality has 3 urban barangays and 4 rural barangays. The study revealed that most of the respondents interviewed were housewives. Out of 313 respondents, 67.10 % did not understand the term natural environment, with major issue currently affecting natural environment as "household garbage'. The respondents dispose the yard trimmings and papers through burning. The plastic and glass is disposed through the garbage truck. The metals and damaged home appliances were disposed by selling it to the junk shop. The respondents disposed their garbage into an open container specifically in a sack, collected by garbage trucks. The study also revealed that the respondents were

  9. Differences between antigenic determinants of pig and cat zona pellucida proteins.

    PubMed

    Jewgenow, K; Rohleder, M; Wegner, I

    2000-05-01

    Despite many efforts, the control of reproduction in feral cat populations is still a problem in urban regions around the world. Immunocontraception is a promising approach; thus the present study examined the suitability of the widely used pig zona pellucida proteins (pZP) for contraception in feral domestic cats. Purified zona pellucida proteins obtained from pig and cat ovaries were used to produce highly specific antisera in rabbits. Antibodies against pZP raised in rabbits or lions were not effective inhibitors of either in vitro sperm binding (cat spermatozoa to cat oocytes) or in vitro fertilization in cats, whereas antibodies against feline zona pellucida proteins (fZP) raised in rabbits showed a dose-dependent inhibition of in vitro fertilization. Immunoelectrophoresis, ELISA and immunohistology of ovaries confirmed these results, showing crossreactivity of anti-fZP sera to fZP and to a lesser extent to pZP, but no interaction of anti-pZP sera with fZP. It is concluded that cat and pig zonae pellucidae express a very small number of shared antigenic determinants, making the use of pZP vaccine in cats questionable. A contraceptive vaccine based on feline zona pellucida determinants will be a better choice for the control of reproduction in feral cats if immunogenity can be achieved.

  10. Urban Impact of Dissolved Metals in the Paso del Norte Segment of the Rio Grande

    ERIC Educational Resources Information Center

    Freiwan, Sumayeh Ahmad

    2013-01-01

    The Paso del Norte segment of the Rio Grande experiences two seasons per year; the (wet) irrigation season and the (dry) non-irrigation season. The goal of this study was to improve the understanding of occurrence and contribution of dissolved metals in this region during the non-irrigation season. The objectives of this study were to (1) evaluate…

  11. ATMOSPHERIC VOLATILE ORGANIC COMPOUND MEASUREMENTS DURING THE 1996 PASO DEL NORTE OZONE STUDY

    EPA Science Inventory

    Ambient air VOC samples were collected at surface air quality monitoring sites, near sources of interest, and aloft on the US (El Paso) and Mexican (Ciudad Juarez) side of the border during a six-week period of the 1996 Paso del Norte Ozone Study. Samples were collected at five...

  12. Assessment of potential shale gas and shale oil resources of the Norte Basin, Uruguay, 2011

    USGS Publications Warehouse

    Schenk, Christopher J.; Kirschbaum, Mark A.; Charpentier, Ronald R.; Cook, Troy; Klett, Timothy R.; Gautier, Donald L.; Pollastro, Richard M.; Weaver, Jean N.; Brownfield, Michael

    2011-01-01

    Using a performance-based geological assessment methodology, the U.S. Geological Survey estimated mean volumes of 13.4 trillion cubic feet of potential technically recoverable shale gas and 0.5 billion barrels of technically recoverable shale oil resources in the Norte Basin of Uruguay.

  13. Determining sex and life stage of Del Norte salamanders from external cues

    Treesearch

    Lisa Ollivier; Hartwell H. Welsh Jr

    2003-01-01

    Life stage determination for many western plethodontids often requires dissection of the specimen. Availability of reliable external measures that could be applied under field conditions would enhance future studies of the genus Plethodon. We examined preserved specimens of the Del Norte Salamander, Plethodon elongatus, taken from...

  14. Diet of the Del Norte Salamander (Plethodon elongatus): Differences by age, gender, and season.

    Treesearch

    Clara A. Wheeler; Nancy E. Karraker; Hartwell H. Welsh; Lisa M. Ollivier

    2007-01-01

    Terrestrial salamanders are integral components of forest ecosystems and the examination of their feeding habits may provide useful information regarding various ecosystem processes. We studied the diet of the Del Norte Salamander (Plethodon elongatus) and assessed diet differences between age classes, genders, and seasons. The stomachs of 309...

  15. The formation of zona radiata in Pseudosciaena crocea revealed by light and transmission electron microscopy.

    PubMed

    Ma, Xiao-Xin; Zhu, Jun-Quan; Zhou, Hong; Yang, Wan-Xi

    2012-02-01

    The egg envelope is an essential structure occurring during oogenesis. It plays an important role during the process of fertilization in the large yellow croaker Pseudosciaena crocea. Elucidation of egg envelope formation helps us to understand fertilization mechanisms in teleosts. In the present work, we studied the formation of egg envelope in P. crocea by light microscopy, as well as by transmission and scanning electron microscopy. Four layers exist outside the oocyte plasmalemma, i.e., theca cell layer, basal membrane, granulosa cell layer and zona radiata. According to our observation, zona radiata is a multilaminar structure just like the same structure reported in teleosts, but the origin of this structure is a little different. Before it is formed, a peripheral space filled with different density of vesicles is the place where zona radiata is formed. Zona radiata (Z1) is secreted only by oocyte itself, it belongs to the primary envelope; zona radiata 2 (Z2) and zona radiata 3 (Z3) belong to the secondary envelope, because the two layers are formed after granulosa cells appear, and microvilli participate this process. It is very interesting that Z2 and Z3 are situated between Z1 and the granulosa cell first, but they translocate to the other side of Z1. This microanatomy difference may due to the participation of microvilli. The new finding about egg envelope formation in P. crocea will help us to do further investigation on fertilization mechanisms and will make artificial breeding possible which may contribute to the resource recovery of this species. Copyright © 2011 Elsevier Ltd. All rights reserved.

  16. Laser assisted zona hatching does not lead to immediate impairment in human embryo quality and metabolism.

    PubMed

    Uppangala, Shubhashree; D'Souza, Fiona; Pudakalakatti, Shivanand; Atreya, Hanudatta S; Raval, Keyur; Kalthur, Guruprasad; Adiga, Satish Kumar

    2016-12-01

    Laser assisted zona hatching (LAH) is a routinely used therapeutic intervention in assisted reproductive technology for patients with poor prognosis. However, results are not conclusive in demonstrating the benefits of zona hatching in improving the pregnancy rate. Recent observations on LAH induced genetic instability in animal embryos prompted us to look into the effects of laser assisted zona hatching on the human preimplantation embryo quality and metabolic uptake using high resolution nuclear magnetic resonance (NMR) technology. This experimental prospective study included fifty embryos from twenty-five patients undergoing intra cytoplasmic sperm injection. Embryo quality assessment followed by profiling of spent media for the non-invasive evaluation of metabolites was performed using NMR spectroscopy 24 hours after laser treatment and compared with that of non-treated sibling embryos. Both cell number and embryo quality on day 3 of development did not vary significantly between the two groups at 24 hours post laser treatment interval. Time lapse monitoring of the embryos for 24 hours did not reveal blastomere fragmentation adjacent to the point of laser treatment. Similarly, principal component analysis of metabolites did not demonstrate any variation across the groups. These results suggest that laser assisted zona hatching does not affect human preimplantation embryo morphology and metabolism at least until 24 hours post laser assisted zona hatching. However, studies are required to elucidate laser induced metabolic and developmental changes at extended time periods. AH: assisted hatching; ART: assisted reproductive technology; DNA: deoxy-ribo nucleic acid; LAH: laser assisted hatching; MHz: megahertz; NMR: nuclear magnetic resonance; PCA: principal component analysis; PGD: preimplantation genetic diagnosis; TLM: time lapse monitoring.

  17. Narrative Report of the El Centro de la Causa Library Project, 1973-1975.

    ERIC Educational Resources Information Center

    Chicago Public Library, IL.

    El Centro de la Causa and the Chicago Public Library cooperated in a program to serve Chicago's Spanish speaking population. The project assessed needs and designed programs to meet those needs by establishing a library in El Centro's community center and some outreach programs. An active acquisitions program for Spanish language materials was…

  18. Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization

    PubMed Central

    Jin, Mayuko; Fujiwara, Eiji; Kakiuchi, Yasutaka; Okabe, Masaru; Satouh, Yuhkoh; Baba, Shoji A.; Chiba, Kazuyoshi; Hirohashi, Noritaka

    2011-01-01

    To fuse with oocytes, spermatozoa of eutherian mammals must pass through extracellular coats, the cumulus cell layer, and the zona pellucida (ZP). It is generally believed that the acrosome reaction (AR) of spermatozoa, essential for zona penetration and fusion with oocytes, is triggered by sperm contact with the zona pellucida. Therefore, in most previous studies of sperm–oocyte interactions in the mouse, the cumulus has been removed before insemination to facilitate the examination of sperm–zona interactions. We used transgenic mouse spermatozoa, which enabled us to detect the onset of the acrosome reaction using fluorescence microscopy. We found that the spermatozoa that began the acrosome reaction before reaching the zona were able to penetrate the zona and fused with the oocyte's plasma membrane. In fact, most fertilizing spermatozoa underwent the acrosome reaction before reaching the zona pellucida of cumulus-enclosed oocytes, at least under the experimental conditions we used. The incidence of in vitro fertilization of cumulus-free oocytes was increased by coincubating oocytes with cumulus cells, suggesting an important role for cumulus cells and their matrix in natural fertilization. PMID:21383182

  19. Fine structure and morphogenesis of spironolactone bodies in the zona glomerulosa of the human adrenal cortex

    PubMed Central

    Kovacs, K.; Horvath, E.; Singer, W.

    1973-01-01

    Numerous spironolactone bodies have been detected in the zona glomerulosa cells of the adrenal cortex of a 36-year-old spironolactone-treated woman whose non-tumorous right adrenal gland was removed surgically because of primary hyperaldosteronism. Electron microscopy revealed spherical laminated whorls which consisted of a central core composed of an amorphous electron-dense material surrounded by numerous smooth-walled concentric membranes. Continuous with and deriving from the endoplasmic reticulum, they were present in viable cells and were not associated with ultrastructural features indicating cellular injury. Cytoplasmic inclusions similar to spironolactone bodies can be detected in other organs after the administration of various compounds. Thus, they can be regarded as neither specific to spironolactone treatment nor exclusively inducible in the zona glomerulosa of the adrenal cortex. Images PMID:4131694

  20. Body mass index is not associated with sperm-zona pellucida binding ability in subfertile males.

    PubMed

    Sermondade, Nathalie; Dupont, Charlotte; Faure, Céline; Boubaya, Marouane; Cédrin-Durnerin, Isabelle; Chavatte-Palmer, Pascale; Sifer, Christophe; Lévy, Rachel

    2013-09-01

    Lifestyle factors, such as weight and nutritional status may affect male fertility, including sperm fertilization ability. The objective of this retrospective study was to evaluate the association between body mass index (BMI) and sperm-zona pellucida binding ability assessed according to the zona binding (ZB) test, which has been described to be a relevant diagnostic tool for the prediction of in vitro fertilization (IVF) ability. Three hundred and six male patients from couples diagnosed with primary idiopathic or mild male factor infertility were included. Correlations between BMI and semen parameters according to ZB test indices were assessed, together with frequencies of positive and negative tests across the BMI categories. In this selected population, BMI was not related to conventional semen parameters or sperm quality assessed according to the ability of spermatozoa to bind to the zona pellucida. The previously described poor outcomes of IVF procedures in cases of male obesity could be due to other sperm defects, such as alterations of sperm capacitation or acrosome reaction. The link between male BMI and biological outcomes during IVF procedures, such as fertilization rates, should be further evaluated.

  1. Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy

    DOE PAGES

    Que, Emily L.; Duncan, Francesca E.; Bayer, Amanda R.; ...

    2017-01-19

    During fertilization or chemically-induced egg activation, the mouse egg releases billions of zinc atoms in brief bursts known as ‘zinc sparks.’ The zona pellucida (ZP), a glycoprotein matrix surrounding the egg, is the first structure zinc ions encounter as they diffuse away from the plasma membrane. Following fertilization, the ZP undergoes changes described as ‘hardening’, which prevent multiple sperm from fertilizing the egg and thereby establish a block to polyspermy. A major event in zona hardening is cleavage of ZP2 proteins by ovastacin; however, the overall physiochemical changes contributing to zona hardening are not well understood. Using x-ray fluorescence microscopy,more » transmission and scanning electron microscopy, and biological function assays, we tested the hypothesis that zinc release contributes to ZP hardening. We found that the zinc content in the ZP increases by 300% following activation and that zinc exposure modulates the architecture of the ZP matrix. Importantly, zinc-induced structural changes of the ZP have a direct biological consequence; namely, they reduce the ability of sperm to bind to the ZP. These results provide a paradigm-shifting model in which fertilization-induced zinc sparks contribute to the polyspermy block by altering conformations of the ZP matrix. Finally, this adds a previously unrecognized factor, namely zinc, to the process of ZP hardening.« less

  2. Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy

    SciTech Connect

    Que, Emily L.; Duncan, Francesca E.; Bayer, Amanda R.

    During fertilization or chemically-induced egg activation, the mouse egg releases billions of zinc atoms in brief bursts known as ‘zinc sparks.’ The zona pellucida (ZP), a glycoprotein matrix surrounding the egg, is the first structure zinc ions encounter as they diffuse away from the plasma membrane. Following fertilization, the ZP undergoes changes described as ‘hardening’, which prevent multiple sperm from fertilizing the egg and thereby establish a block to polyspermy. A major event in zona hardening is cleavage of ZP2 proteins by ovastacin; however, the overall physiochemical changes contributing to zona hardening are not well understood. Using x-ray fluorescence microscopy,more » transmission and scanning electron microscopy, and biological function assays, we tested the hypothesis that zinc release contributes to ZP hardening. We found that the zinc content in the ZP increases by 300% following activation and that zinc exposure modulates the architecture of the ZP matrix. Importantly, zinc-induced structural changes of the ZP have a direct biological consequence; namely, they reduce the ability of sperm to bind to the ZP. These results provide a paradigm-shifting model in which fertilization-induced zinc sparks contribute to the polyspermy block by altering conformations of the ZP matrix. Finally, this adds a previously unrecognized factor, namely zinc, to the process of ZP hardening.« less

  3. Thermal effects in laser-assisted pre-embryo zona drilling

    NASA Astrophysics Data System (ADS)

    Douglas-Hamilton, Diarmaid H.; Conia, Jerome D.

    2001-04-01

    Diode lasers ((lambda) equals 1480 nm) are used with in vitro fertilization to dissect the zone pellucida (shell) of pre- embryos. A focused laser beam is applied in vitro to form a channel or trench in the zona pellucida. The procedure is used to facilitate biopsy or as a promoter of embryo hatching. We present examples and measurements of zona pellucida ablation using animal models. In using the laser it is vital not to damage pre-embryo cells, e.g., by overheating. In order to define safe regimes we have derived some thermal side effects of zona pellucida removal. The temperature profile in the beam and vicinity is predicted as function of laser pulse duration and power. In a crossed- beam experiment a HeNe laser probe is used to detect the temperature-induced change in the refractive index of an aqueous solution, and estimate local thermal gradient. We find that the diode laser beam produces superheated water approaching 200 degree(s)C on the beam axis. Thermal histories during and following the laser pulse are given for regions in the neighborhood of the beam. We conclude that an optimum regime exists with pulse duration

  4. Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep

    PubMed Central

    Liu, Kai; Kim, Juhyun; Kim, Dong Won; Zhang, Yi Stephanie; Bao, Hechen; Denaxa, Myrto; Lim, Szu-Aun; Kim, Eileen; Liu, Chang; Wickersham, Ian R.; Pachnis, Vassilis; Hattar, Samer; Song, Juan; Brown, Solange P.; Blackshaw, Seth

    2017-01-01

    Multiple populations of wake-promoting neurons have been characterized in mammals, but few sleep-promoting neurons have been identified1. Wake-promoting cell types include hypocretin and GABA (γ-aminobutyric-acid)-releasing neurons of the lateral hypothalamus, which promote the transition to wakefulness from non-rapid eye movement (NREM) and rapid eye movement (REM) sleep2,3. Here we show that a subset of GABAergic neurons in the mouse ventral zona incerta, which express the LIM homeodomain factor Lhx6 and are activated by sleep pressure, both directly inhibit wake-active hypocretin and GABAergic cells in the lateral hypothalamus and receive inputs from multiple sleep–wake-regulating neurons. Conditional deletion of Lhx6 from the developing diencephalon leads to decreases in both NREM and REM sleep. Furthermore, selective activation and inhibition of Lhx6-positive neurons in the ventral zona incerta bidirectionally regulate sleep time in adult mice, in part through hypocretin-dependent mechanisms. These studies identify a GABAergic subpopulation of neurons in the ventral zona incerta that promote sleep. PMID:28847002

  5. Laser-assisted zona pellucida thinning does not facilitate hatching and may disrupt the in vitro hatching process: a morphokinetic study in the mouse.

    PubMed

    Schimmel, Tim; Cohen, Jacques; Saunders, Helen; Alikani, Mina

    2014-12-01

    Does laser-assisted zona thinning of cleavage stage mouse embryos facilitate hatching in vitro? No, unlike laser zona opening, zona thinning does not facilitate embryo hatching. Artificial opening of the zona pellucida facilitates hatching of mouse and human embryos. Laser-assisted zona thinning has also been used for the purpose of assisted hatching of human embryos but it has not been properly investigated in an animal model; thinning methods have produced inconsistent clinical results. Time-lapse microscopy was used to study the hatching process in the mouse after zona opening and zona thinning; a control group of embryos was not zona-manipulated but exposed to the same laser energy. Eight-cell CB6F1/J mouse embryos were pooled and allocated to three groups (n = 56 per group): A control group of embryos that were exposed to a dose of laser energy focused outside the zona pellucida (zona intact); one experimental group of embryos in which the zona pellucida was opened by complete ablation using the same total number of pulses as the control group; a second experimental group of embryos in which the zona pellucida was thinned to establish a smooth lased area using the same number of pulses as used in the other two groups. The width of the zona opening was 25 μm and width of the thinned area was 35 μm. Development was monitored by time-lapse microscopy. Overall treatment differences for continuous variables were analyzed by analysis of variance and pairwise comparisons using the Student t-test allowing for unequal variances, while for categorical data, a standard chi-squared test was utilized for all pairwise comparisons. The frequency of complete hatching was 33.9% in the control group, 94.4% after zona opening, and 39.3% after zona thinning (overall group comparison, P < 0.0001). Overall, 60.7% of the zona-thinned embryos did not complete the hatching process and remained trapped within the zona; when they did hatch, they did not necessarily hatch from the zona

  6. Sperm Proteasomes Degrade Sperm Receptor on the Egg Zona Pellucida during Mammalian Fertilization

    PubMed Central

    Zimmerman, Shawn W.; Manandhar, Gaurishankar; Yi, Young-Joo; Gupta, Satish K.; Sutovsky, Miriam; Odhiambo, John F.; Powell, Michael D.; Miller, David J.; Sutovsky, Peter

    2011-01-01

    Despite decades of research, the mechanism by which the fertilizing spermatozoon penetrates the mammalian vitelline membrane, the zona pellucida (ZP) remains one of the unexplained fundamental events of human/mammalian development. Evidence has been accumulating in support of the 26S proteasome as a candidate for echinoderm, ascidian and mammalian egg coat lysin. Monitoring ZP protein degradation by sperm during fertilization is nearly impossible because those few spermatozoa that penetrate the ZP leave behind a virtually untraceable residue of degraded proteins. We have overcome this hurdle by designing an experimentally consistent in vitro system in which live boar spermatozoa are co-incubated with ZP-proteins (ZPP) solubilized from porcine oocytes. Using this assay, mimicking sperm-egg interactions, we demonstrate that the sperm-borne proteasomes can degrade the sperm receptor protein ZPC. Upon coincubation with motile spermatozoa, the solubilized ZPP, which appear to be ubiquitinated, adhered to sperm acrosomal caps and induced acrosomal exocytosis/formation of the acrosomal shroud. The degradation of the sperm receptor protein ZPC was assessed by Western blotting band-densitometry and proteomics. A nearly identical pattern of sperm receptor degradation, evident already within the first 5 min of coincubation, was observed when the spermatozoa were replaced with the isolated, enzymatically active, sperm-derived proteasomes. ZPC degradation was blocked by proteasomal inhibitors and accelerated by ubiquitin-aldehyde(UBAL), a modified ubiquitin protein that stimulates proteasomal proteolysis. Such a degradation pattern of ZPC is consistent with in vitro fertilization studies, in which proteasomal inhibitors completely blocked fertilization, and UBAL increased fertilization and polyspermy rates. Preincubation of intact zona-enclosed ova with isolated active sperm proteasomes caused digestion, abrasions and loosening of the exposed zonae, and significantly reduced

  7. Identification of alpha-enolase as a nuclear DNA-binding protein in the zona fasciculata but not the zona reticularis of the human adrenal cortex.

    PubMed

    Wang, Weiye; Wang, Lishan; Endoh, Akira; Hummelke, Geoffrey; Hawks, Christina L; Hornsby, Peter J

    2005-01-01

    In order to establish whether there are differences in DNA-binding proteins between zona fasciculata (ZF) and zona reticularis (ZR) cells of the human adrenal cortex, we prepared nuclear extracts from separated ZF and ZR cells. The formation of DNA-protein complexes was studied using an element in the first intron of the type I and type II 3beta-hydroxysteroid dehydrogenase genes (HSD3B1 and HSD3B2). Using the element in the HSD3B2 gene as a probe, a complex (C1) was formed with extracts from ZF cells but was formed only at a low level with ZR cell extracts. Another pair of complexes (C2/C3) was formed with both ZF and ZR cell extracts. The ZF-specific protein forming C1 was enriched by column chromatography on DEAE-Sepharose and carboxymethyl-Sepharose. Oligonucleotide competition analysis on the enriched fraction gave results consistent with those obtained on the unfractionated material. A further enrichment was brought about by passing the protein over an oligonucleotide affinity column based on the HSD3B2 element. The protein bound to the column was identified as alpha-enolase by mass spectrometry. Although alpha-enolase is a glycolytic enzyme, it binds to specific DNA sequences and has been found to be present in nuclei of various cell types. We performed immunohistochemistry on sections of adult human adrenal cortex and found alpha-enolase to be located in nuclei of ZF cells but to be predominantly cytoplasmic in ZR cells. Transfection of an alpha-enolase expression vector into NCI-H295R human adrenocortical cells increased HSD3B2 promoter activity, suggesting a possible functional role for this protein in regulation of HSD3B2 expression.

  8. Elevation and mass change of the Echaurren Norte Glacier (Central Andes, Chile) from 1955 to 2015.

    NASA Astrophysics Data System (ADS)

    Farías, David; Vivero, Sebastián; Casassa, Gino; Seehaus, Thorsten; Braun, Matthias H.

    2017-04-01

    The Echaurren Norte Glacier (33°34'S 70°07'W) is a small mountain glacier located at the upper Maipo basin, approximately 80 km to Santiago de Chile. The glacier has the longest surface mass balance record in South America (1975 to 2016). The measurements are carried out by DGA (water directory of Chile) using the direct glaciological method. The surface mass balance show continuous negative values, but exceptional positive mass balances were identified during ENSO periods. The aim of our study is complement the in-situ observations on Echaurren Norte Glacier with the geodetic mass balance measurements for the period 1955 to 2015. Our database comprises digital elevation models (DEM) from historical cartography based on aerial photographs (1955), SRTM (2000) and Lidar data. In addition, we mapped changes in glacier extent using aerial photography and multi-mission satellite data. TanDEM-X (2012-2015) and SRTM data will be used to investigate surrounding glaciers that have not such extensive and detailed coverage as Echaurren Norte Glacier. The aerial photographs from 1955 were scanned from the original negative using a photogrammetric scanner and processed on a digital photogrammetric workstation (DPW) and georeferenced with the aid of GCPs derived from the Lidar dataset. The TanDEM-X data was processed using differential interferometry using SRTM C-band DEM as reference. Differences resulting from X- and C-band penetration are considered comparing X- and C-band SRTM data. All DEMs were laterally and vertically co-registered to each other. Error assessment was done over stable ground. Our preliminary results indicate an elevation change of -42.2 m ± 4 m (1955-2015) for Echaurren Norte Glacier. The estimated averaged annual mass balance is -0.59 m water equivalent for the period 1955-2015 using a density of 0.85 kg/cm3 for volume to mass conversion. Significant changes of the surface cover were identified, with a considerable increase of the debris cover, in

  9. Pentoxifylline increases sperm penetration into zona-free hamster oocytes without increasing the acrosome reaction.

    PubMed

    Morales, P; Llanos, M; Yovich, J L; Cummins, J M; Vigil, P

    1993-01-01

    Several drugs have been used to stimulate human sperm motility, including 3-deoxy-adenosine, caffeine, and pentoxifylline. Pentoxifylline is an inhibitor of the phosphodiesterase and may stimulate sperm motility by increasing the intracellular levels of cAMP. In this study we have evaluated the effect of pentoxifylline in the outcome of the sperm penetration assay into zona-free hamster oocytes. Twenty-seven semen samples, obtained for diagnostic purposes, were used. After the motile sperm were selected by the swim-up technique, the samples were divided into two aliquots. One aliquot was incubated with 1 mg ml-1 of pentoxifylline at 37 degrees C, 5% CO2 for 30 min. The control aliquot was incubated with culture medium. The samples were then washed and resuspended in fresh, pentoxifylline-free medium, at a sperm concentration of 10 x 10(6) cells ml-1. One hundred microlitres of each sperm suspension was then deposited under oil and 30-40 zona-free hamster oocytes were added. After 6 h of gamete coincubation, the percentage of penetrated oocytes and the number of decondensed sperm heads were evaluated. The percentage of acrosome-reacted sperm was evaluated using the Pisum sativum lectin. The percentage of zona-free hamster oocytes penetrated was increased after pentoxifylline-treatment. The percentage of acrosome reacted sperm and the number of decondensed sperm heads per egg were not different between the control and the pentoxifylline-treated groups. The results suggest that the beneficial effect of pentoxifylline upon the sperm cells is not mediated by stimulation of the acrosome reaction.

  10. Human sperm bind to the N-terminal domain of ZP2 in humanized zonae pellucidae in transgenic mice

    PubMed Central

    Baibakov, Boris; Boggs, Nathan A.; Yauger, Belinda; Baibakov, Galina

    2012-01-01

    Fertilization requires taxon-specific gamete recognition, and human sperm do not bind to zonae pellucidae (ZP1–3) surrounding mouse eggs. Using transgenesis to replace endogenous mouse proteins with human homologues, gain-of-function sperm-binding assays were established to evaluate human gamete recognition. Human sperm bound only to zonae pellucidae containing human ZP2, either alone or coexpressed with other human zona proteins. Binding to the humanized matrix was a dominant effect that resulted in human sperm penetration of the zona pellucida and accumulation in the perivitelline space, where they were unable to fuse with mouse eggs. Using recombinant peptides, the site of gamete recognition was located to a defined domain in the N terminus of ZP2. These results provide experimental evidence for the role of ZP2 in mediating sperm binding to the zona pellucida and support a model in which human sperm–egg recognition is dependent on an N-terminal domain of ZP2, which is degraded after fertilization to provide a definitive block to polyspermy. PMID:22734000

  11. Automation and Optimization of Multipulse Laser Zona Drilling of Mouse Embryos During Embryo Biopsy.

    PubMed

    Wong, Christopher Yee; Mills, James K

    2017-03-01

    Laser zona drilling (LZD) is a required step in many embryonic surgical procedures, for example, assisted hatching and preimplantation genetic diagnosis. LZD involves the ablation of the zona pellucida (ZP) using a laser while minimizing potentially harmful thermal effects on critical internal cell structures. Develop a method for the automation and optimization of multipulse LZD, applied to cleavage-stage embryos. A two-stage optimization is used. The first stage uses computer vision algorithms to identify embryonic structures and determines the optimal ablation zone farthest away from critical structures such as blastomeres. The second stage combines a genetic algorithm with a previously reported thermal analysis of LZD to optimize the combination of laser pulse locations and pulse durations. The goal is to minimize the peak temperature experienced by the blastomeres while creating the desired opening in the ZP. A proof of concept of the proposed LZD automation and optimization method is demonstrated through experiments on mouse embryos with positive results, as adequately sized openings are created. Automation of LZD is feasible and is a viable step toward the automation of embryo biopsy procedures. LZD is a common but delicate procedure performed by human operators using subjective methods to gauge proper LZD procedure. Automation of LZD removes human error to increase the success rate of LZD. Although the proposed methods are developed for cleavage-stage embryos, the same methods may be applied to most types LZD procedures, embryos at different developmental stages, or nonembryonic cells.

  12. Dynamic regulation of sperm interactions with the zona pellucida prior to and after fertilisation.

    PubMed

    Gadella, B M

    2012-01-01

    Recent findings have refined our thinking on sperm interactions with the cumulus-oocyte complex (COC) and our understanding of how, at the molecular level, the sperm cell fertilises the oocyte. Proteomic analyses has identified a capacitation-dependent sperm surface reordering that leads to the formation of functional multiprotein complexes involved in zona-cumulus interactions in several mammalian species. During this process, multiple docking of the acrosomal membrane to the plasma membrane takes place. In contrast with the dogma that the acrosome reaction is initiated when spermatozoa bind to the zona pellucida (ZP), it has been established recently that, in mice, the fertilising spermatozoon initiates its acrosome reaction during its voyage through the cumulus before it reaches the ZP. In fact, even acrosome-reacted mouse spermatozoa collected from the perivitelline space can fertilise another ZP-intact oocyte. The oviduct appears to influence the extracellular matrix properties of the spermatozoa as well as the COC. This may influence sperm binding and penetration of the cumulus and ZP, and, in doing so, increase monospermic while decreasing polyspermic fertilisation rates. Structural analysis of the ZP has shed new light on how spermatozoa bind and penetrate this structure and how the cortical reaction blocks sperm-ZP interactions. The current understanding of sperm interactions with the cumulus and ZP layers surrounding the oocyte is reviewed with a special emphasis on the lack of comparative knowledge on this topic in humans, as well as in most farm mammals.

  13. Functional TASK-3-Like Channels in Mitochondria of Aldosterone-Producing Zona Glomerulosa Cells.

    PubMed

    Yao, Junlan; McHedlishvili, David; McIntire, William E; Guagliardo, Nick A; Erisir, Alev; Coburn, Craig A; Santarelli, Vincent P; Bayliss, Douglas A; Barrett, Paula Q

    2017-08-01

    Ca 2+ drives aldosterone synthesis in the cytosolic and mitochondrial compartments of the adrenal zona glomerulosa cell. Membrane potential across each of these compartments regulates the amplitude of the Ca 2+ signal; yet, only plasma membrane ion channels and their role in regulating cell membrane potential have garnered investigative attention as pathological causes of human hyperaldosteronism. Previously, we reported that genetic deletion of TASK-3 channels (tandem pore domain acid-sensitive K + channels) from mice produces aldosterone excess in the absence of a change in the cell membrane potential of zona glomerulosa cells. Here, we report using yeast 2-hybrid, immunoprecipitation, and electron microscopic analyses that TASK-3 channels are resident in mitochondria, where they regulate mitochondrial morphology, mitochondrial membrane potential, and aldosterone production. This study provides proof of principle that mitochondrial K + channels, by modulating inner mitochondrial membrane morphology and mitochondrial membrane potential, have the ability to play a pathological role in aldosterone dysregulation in steroidogenic cells. © 2017 American Heart Association, Inc.

  14. 3 CFR 8946 - Proclamation 8946 of March 25, 2013. Establishment of the Río Grande del Norte National Monument

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... have seasonally passed through the Río Grande del Norte, gathering resources and finding spiritual... covering the mountain's northern slopes, the diversity of both ecosystems and species allows for, and has..., and American avocets. Several species of bats make their home in the gorge, which also provides...

  15. Human migration, railways and the geographic distribution of leprosy in Rio Grande do Norte State – Brazil

    PubMed Central

    Nobre, Mauricio Lisboa; Dupnik, Kathryn Margaret; Nobre, Paulo José Lisboa; De Souza, Márcia Célia Freitas; Dűppre, Nádia Cristina; Sarno, Euzenir Nunes; Jerŏnimo, Selma Maria Bezerra

    2016-01-01

    Summary Introduction Leprosy is a public health problem in Brazil where 31,044 new cases were detected in 2013. Rio Grande do Norte is a small Brazilian state with a rate of leprosy lower than other areas in the same region, for unknown reasons. Objectives We present here a review based on the analysis of a database of registered leprosy cases in Rio Grande do Norte state, comparing leprosy's geographic distribution among municipalities with local socio-economic and public health indicators and with historical documents about human migration in this Brazilian region. Results The current distribution of leprosy in Rio Grande do Norte did not show correlation with socio-economic or public health indicators at the municipal level, but it appears related to economically emerging municipalities 100 years ago, with spread facilitated by railroads and train stations. Drought-related migratory movements which occurred from this state to leprosy endemic areas within the same period may be involved in the introduction of leprosy and with its present distribution within Rio Grande do Norte. Conclusions Leprosy may disseminate slowly, over many decades in certain circumstances, such as in small cities with few cases. This is a very unusual situation currently and a unique opportunity for epidemiologic studies of leprosy as an emerging disease. PMID:26964429

  16. Willingness to Pay for Dog Rabies Vaccine and Registration in Ilocos Norte, Philippines (2012).

    PubMed

    Birhane, Meseret G; Miranda, Mary Elizabeth G; Dyer, Jessie L; Blanton, Jesse D; Recuenco, Sergio

    2016-03-01

    The Philippines is one of the developing countries highly affected by rabies. Dog vaccination campaigns implemented through collaborative effort between the government and NGOs have played an important role in successfully reducing the burden of disease within the country. Nevertheless, rabies vaccination of the domestic animal population requires continuous commitment not only from governments and NGOs, but also from local communities that are directly affected by such efforts. To create such long-term sustained programs, the introduction of affordable dog vaccination and registration fees is essential and has been shown to be an important strategy in Bohol, Philippines. The aim of this study, therefore, was to estimate the average amount of money that individuals were willing to pay for dog vaccination and registration in Ilocos Norte, Philippines. This study also investigated some of the determinants of individuals' willingness to pay (WTP). A cross-sectional questionnaire was administered to 300 households in 17 municipalities (out of a total of 21) selected through a multi-stage cluster survey technique. At the time of the survey, Ilocos Norte had a population of approximately 568,017 and was predominantly rural. The Contingent Valuation Method was used to elicit WTP for dog rabies vaccination and registration. A 'bidding game' elicitation strategy that aims to find the maximum amount of money individuals were willing to pay was also employed. Data were collected using paper-based questionnaires. Linear regression was used to examine factors influencing participants' WTP for dog rabies vaccination and registration. On average, Ilocos Norte residents were willing to pay 69.65 Philippine Pesos (PHP) (equivalent to 1.67 USD in 2012) for dog vaccination and 29.13PHP (0.70 USD) for dog registration. Eighty-six per cent of respondents were willing to pay the stated amount to vaccinate each of their dogs, annually. This study also found that WTP was influenced by

  17. Willingness to Pay for Dog Rabies Vaccine and Registration in Ilocos Norte, Philippines (2012)

    PubMed Central

    Birhane, Meseret G.; Miranda, Mary Elizabeth G.; Dyer, Jessie L.; Blanton, Jesse D.; Recuenco, Sergio

    2016-01-01

    Background The Philippines is one of the developing countries highly affected by rabies. Dog vaccination campaigns implemented through collaborative effort between the government and NGOs have played an important role in successfully reducing the burden of disease within the country. Nevertheless, rabies vaccination of the domestic animal population requires continuous commitment not only from governments and NGOs, but also from local communities that are directly affected by such efforts. To create such long-term sustained programs, the introduction of affordable dog vaccination and registration fees is essential and has been shown to be an important strategy in Bohol, Philippines. The aim of this study, therefore, was to estimate the average amount of money that individuals were willing to pay for dog vaccination and registration in Ilocos Norte, Philippines. This study also investigated some of the determinants of individuals’ willingness to pay (WTP). Methods A cross-sectional questionnaire was administered to 300 households in 17 municipalities (out of a total of 21) selected through a multi-stage cluster survey technique. At the time of the survey, Ilocos Norte had a population of approximately 568,017 and was predominantly rural. The Contingent Valuation Method was used to elicit WTP for dog rabies vaccination and registration. A ‘bidding game’ elicitation strategy that aims to find the maximum amount of money individuals were willing to pay was also employed. Data were collected using paper-based questionnaires. Linear regression was used to examine factors influencing participants’ WTP for dog rabies vaccination and registration. Key Results On average, Ilocos Norte residents were willing to pay 69.65 Philippine Pesos (PHP) (equivalent to 1.67 USD in 2012) for dog vaccination and 29.13PHP (0.70 USD) for dog registration. Eighty-six per cent of respondents were willing to pay the stated amount to vaccinate each of their dogs, annually. This study

  18. Anticipation of somatosensory and motor events increases centro-parietal functional coupling: an EEG coherence study.

    PubMed

    Babiloni, Claudio; Brancucci, Alfredo; Vecchio, Fabrizio; Arendt-Nielsen, Lars; Chen, Andrew C N; Rossini, Paolo M

    2006-05-01

    Does functional coupling of centro-parietal EEG rhythms selectively increase during the anticipation of sensorimotor events composed by somatosensory stimulation and visuomotor task? EEG data were recorded in (1) 'simultaneous' condition in which the subjects waited for somatosensory stimulation at left hand concomitant with a Go (or NoGo) visual stimulus triggering (50%) right hand movements and in (2) 'sequential' condition where the somatosensory stimulation was followed (+1.5 s) by a visuomotor Go/NoGo task. Centro-parietal functional coupling was modeled by spectral coherence. Spectral coherence was computed from Laplacian-transformed EEG data at delta-theta (2-7 Hz), alpha (8-14 Hz), beta 1 (15-21 Hz), beta 2 (22-33 Hz), and gamma (34-45 Hz) rhythms. Before 'simultaneous' sensorimotor events, centro-parietal coherence regions increased in both hemispheres and at all rhythms. In the 'sequential' condition, right centro-parietal coherence increased before somatosensory event (left hand), whereas left centro-parietal coherence increased before subsequent Go/NoGo event (right hand). Anticipation of somatosensory and visuomotor events enhances contralateral centro-parietal coupling of slow and fast EEG rhythms. Predictable somatosensory and visuomotor events are anticipated not only by synchronization of cortical pyramidal neurons generating EEG power in parietal and primary sensorimotor cortical areas (Babiloni C, Brancucci A, Capotosto P, Arendt-Nielsen L, Chen ACN, Rossini PM. Expectancy of pain is influenced by motor preparation: a high-resolution EEG study of cortical alpha rhythms. Behav. Neurosci. 2005a;119(2):503-511; Babiloni C, Brancucci A, Pizzella V, Romani G.L, Tecchio F, Torquati K, Zappasodi F, Arendt-Nielsen L, Chen ACN, Rossini PM. Contingent negative variation in the parasylvian cortex increases during expectancy of painful sensorimotor events: a magnetoencephalographic study. Behav. Neurosci. 2005b;119(2):491-502) but also by functional

  19. Gastrointestinal parasites of sheep, municipality of Lajes, Rio Grande do Norte, Brazil.

    PubMed

    de Souza, Maria de Fátima; Pimentel-Neto, Manoel; da Silva, Rízia Maria; Farias, Albeísa Cleyse Batista; Guimarães, Marcos Pezzi

    2012-01-01

    This study investigated the gastrointestinal parasitism by helminths and protozoa in sheep (Ovis aries) Santa Inês breed, municipality of Lajes, Rio Grande do Norte. Monthly, from April 2005 to August 2007, stool samples were collected from two tracer lambs in the first day of the experiment and performed a necropsy of these animals in 44th day. A total of 64 lambs were sampled, but only 62 lambs were slaughtered. The fecal samples were examined by sedimentation in water. The contents of the abomasum, small intestine and large intestine were examined for the recovery of helminths. The parasitological examination revealed eggs of the following groups of helminths: Strongyloidea, Strongyloides sp., Trichuris sp., and Moniezia sp. Also were found oocysts of Eimeria spp., cysts of Entamoeba ovis and Giardia duodenalis. The helminths identified from examining the contents were: Haemonchus contortus, Cooperia pectinata, Cooperia punctata, Trichostrongylus colubriformis, Moniezia expansa, Oesophagostomum sp. Skrjabinema ovis and Trichuris sp.

  20. [Fragments of history in psychiatric care Rio Grande do Norte, Brazil].

    PubMed

    de Miranda, Francisco Arnoldo Nunes; Santos, Raionara Cristina de Araújo; de Azevedo, Dulcian Medeiros; Fernandes, Rafaella Leite; Costa, Tarciana Sampaio

    2010-09-01

    This article aims to rescue aspects of the performing therapeutic of the Day Hospital (HD) Dr Elger Nunes, Natal, Rio Grande do Norte, Brazil, during its term, and analyze the results regarding to the number of patients assisted from 1996 to 2004. This is an empirical, descriptive and exploratory study, ex post facto with a quantitative approach, carried out through the analysis of the records of 910 people attended in the hospital. The data was submitted to the informational resource software Microsoft Excel and converted into diagrams. The results show a greater accessibility to this treatment modality, decreasing in hospitalization-time length and improving hospital discharge conditions for users, with reduction in number of patients who interrupted treatment. It focus on the importance of the Day Hospital in the process of psychiatric reform, with care grounded on the use of the humanized therapeutic practices, and still not losing the bond with family and society.

  1. Utilization of LANDSAT orbital imagery in the soil survey processes at Rio Grande do Norte state

    NASA Technical Reports Server (NTRS)

    Formaggio, A. R. (Principal Investigator)

    1984-01-01

    Pedologic photointerpretative criteria adapted to LANDSAT orbital imagery were used: drainage (pattern, integration degree, density and uniformity degree); relief (pattern, dissection degree and crest lines); photographic texture, photographic tonnality, and the land use (type, glebas size and intensity of use). The performance of the imagery as an auxiliar tool in the soil survey processes, at Rio Grande do Norte State was evaluated. The drainage and relief elements were easily extracted from the imagery and also ones that provided the greatest deductive possibility about pedologic boundaries. Other analyzed criteria were considered only auxiliaries, corroborating some soil limits in the evidences convergence phase. The principal pedologic dominions of the 30,000 sq km are covered by the same LANDSAT image (WRS 359/16) were delimited with good precision: (1) fluvial plains, beaches, dunes and coastal mangroves; (2) North Coast line Plateau; (3) Acu Sandstone Zone; (4) residual plateaus of the Tertiary; and (6) plains of the embasement.

  2. Geology and depositional environments of the Guadalupian rocks of the northern Del Norte Mountains, West Texas

    USGS Publications Warehouse

    Rudine, S.F.; Wardlaw, B.R.; Rohr, D.M.; Grant, R.E.

    2000-01-01

    The Guadalupian rocks of the northern Del Norte Mountains were deposited in a foreland basin between land of the Marathon orogen and a carbonate shoal established on the geanticline separating the foreland basin from the Delaware basin. Deposition was alternately influenced by coarse clastic input from the orogen and carbonate shoal, which interrupted shallow basinal siltstone depletion. Relatively deeper-water deposition is characterized by carbonate input from the shoal, and relatively shallow-water deposition is characterized by sandstone input from the orogen. Deposition was in five general transgressive-regressive packages that include (1) the Road Canyon Formation and the first siltstone member and first sandstone member of the Word Formation, (2) the second siltstone member, Appel Ranch Member, and limy sandy siltstone member of the Word Formation, (3) the Vidrio Formation, (4) the lower and part of the middle members of the Altuda Formation, and (5) part of the middle and upper members of the Altuda Formation.

  3. Zona pellucida gene mRNA expression in human oocytes is related to oocyte maturity, zona inner layer retardance and fertilization competence.

    PubMed

    Canosa, S; Adriaenssens, T; Coucke, W; Dalmasso, P; Revelli, A; Benedetto, C; Smitz, J

    2017-05-01

    Do the mRNA expression levels of zona pellucida (ZP) genes, ZP1, 2, 3 and 4 in oocyte and cumulus cells (CC) reveal relevant information on the oocyte? The ZP mRNA expression in human oocytes is related to oocyte maturity, zona inner layer (IL) retardance and fertilization capacity. ZP structure and birefringence provide useful information on oocyte cytoplasmic maturation, developmental competence for embryonic growth, blastocyst formation and pregnancy. In order to understand the molecular basis of morphological changes in the ZP, in the current study, the polarized light microscopy (PLM) approach was combined with analysis of the expression of the genes encoding ZP1, 2, 3 and 4, both in the oocytes and in the surrounding CC. This is a retrospective study comprising 98 supernumerary human cumulus oocyte complexes (COC) [80 Metaphase II (MII), 10 Metaphase I (MI) and 8 germinal vesicle (GV)] obtained from 39 patients (median age 33.4 years, range 22-42) after controlled ovarian stimulation. Single oocytes and their corresponding CC were analysed. Oocytes were examined using PLM, and quantitative RT-PCR was performed for ZP1, 2, 3 and 4 in these individual oocytes and their CC. Ephrin-B2 (EFNB2) mRNA was measured in CC as a control. Presence of ZP3 protein in CC and oocytes was investigated using immunocytochemistry. Data were analysed using one-parametric and multivariate analysis and were corrected for the potential impact of patient and cycle characteristics. Oocytes contained ZP1/2/3 and 4 mRNA while in CC only ZP3 was quantifiable. Also ZP3 protein was detected in human CC. When comparing mature (MII) and immature oocytes (MI/GV) or their corresponding CC, ZP1/2 and 4 expression was lower in mature oocytes compared to the expression in immature oocytes (all P < 0.05) and ZP3 expression was lower in the CC of mature oocytes compared to the expression in CC of immature oocytes (P < 0.05). This coincided with a significantly smaller IL-ZP area and thickness in

  4. Rapid binge-like eating and body weight gain driven by zona incerta GABA neuron activation.

    PubMed

    Zhang, Xiaobing; van den Pol, Anthony N

    2017-05-26

    The neuronal substrate for binge eating, which can at times lead to obesity, is not clear. We find that optogenetic stimulation of mouse zona incerta (ZI) γ-aminobutyric acid (GABA) neurons or their axonal projections to paraventricular thalamus (PVT) excitatory neurons immediately (in 2 to 3 seconds) evoked binge-like eating. Minimal intermittent stimulation led to body weight gain; ZI GABA neuron ablation reduced weight. ZI stimulation generated 35% of normal 24-hour food intake in just 10 minutes. The ZI cells were excited by food deprivation and the gut hunger signal ghrelin. In contrast, stimulation of excitatory axons from the parasubthalamic nucleus to PVT or direct stimulation of PVT glutamate neurons reduced food intake. These data suggest an unexpected robust orexigenic potential for the ZI GABA neurons. Copyright © 2017, American Association for the Advancement of Science.

  5. Specific antibodies to porcine zona pellucida detected by quantitative radioimmunoassay in both fertile and infertile women

    SciTech Connect

    Kurachi, H.; Wakimoto, H.; Sakumoto, T.

    1984-02-01

    The specific radioimmunoassay system was developed for the titration of the antibodies to porcine zona pellucida (ZP) in human sera by using /sup 125/I-labeled purified porcine ZP as antigen, which is known to have cross-reactivity with human ZP. The antibodies in human sera were detected in 3 of 11 (27%) women with unexplained infertility, in 16 of 48 (33%) amenorrheic patients, in 4 of 12 (33%) fertile women, and in 3 of 10 (30%) men. Moreover, antibody titers in infertile women were no higher than those in fertile women and in men. These results seem to suggest that the antibodiesmore » in human sera that cross-react with porcine ZP may not be an important factor in causing infertility in women.« less

  6. [Four stages in the history of the Centro Médico Nacional Siglo XXI of the IMSS].

    PubMed

    Fajardo-Ortiz, Guillermo

    2015-01-01

    This document presents four stages in the history of the Centro Médico Nacional Siglo XXI (Centro Médico Nacional XXI Century) of the Instituto Mexicano del Seguro Social. The first stage started at the end of the third decade of the twentieth century and ended in 1961, it corresponded to the conception, planning and construction of what was to be the Centro Médico del Distrito Federal (Centro Médico of the Distrito Federal) belonging to the Secretaría de Salubridad y Asistencia (Ministry of Health and Assistance). The second stage began when the Center was acquired by the Institute, then was known like Centro Médico Nacional (Centro Médico Nacional ), being put into full operation in 1963, more than twenty-two years later, in 1985, an earthquake virtually ended it, immediately began its reconstruction, finishing the second stage. In 1989 began the third stage, different and new buildings complemented or replaced the structures damaged or destroyed by the earthquake which formed the now Centro Médico Nacional Siglo XXI (Centro Médico Nacional XXI Century). In 2004 the fourth stage opened when the four hospitals of the Center were categorized like Unidades Médicas de Alta Especialidad (High Specialized Medical Units).

  7. Structural Analysis of Peptide-Analogues of Human Zona Pellucida ZP1 Protein with Amyloidogenic Properties: Insights into Mammalian Zona Pellucida Formation

    PubMed Central

    Louros, Nikolaos N.; Iconomidou, Vassiliki A.; Giannelou, Polina; Hamodrakas, Stavros J.

    2013-01-01

    Zona pellucida (ZP) is an extracellular matrix surrounding and protecting mammalian and fish oocytes, which is responsible for sperm binding. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. These proteins polymerize into long interconnected filaments, through a common structural unit, known as the ZP domain, which consists of two domains, ZP-N and ZP-C. ZP is related in function to silkmoth chorion and in an evolutionary fashion to the teleostean fish chorion, also fibrous structures protecting the oocyte and embryo, that both have been proven to be functional amyloids. Two peptides were predicted as ‘aggregation-prone’ by our prediction tool, AMYLPRED, from the sequence of the human ZP1-N domain. Here, we present results from transmission electron microscopy, X-ray diffraction, Congo red staining and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FT-IR), of two synthetic peptide-analogues of these predicted ‘aggregation-prone’ parts of the human ZP1-N domain, that we consider crucial for ZP protein polymerization, showing that they both self-assemble into amyloid-like fibrils. Based on our experimental data, we propose that human ZP (hZP) might be considered as a novel, putative, natural protective amyloid, in close analogy to silkmoth and teleostean fish chorions. Experiments are in progress to verify this proposal. We also attempt to provide insights into ZP formation, proposing a possible model for hZP1-N domain polymerization. PMID:24069181

  8. Tiger, Bengal and Domestic Cat Embryos Produced by Homospecific and Interspecific Zona-Free Nuclear Transfer.

    PubMed

    Moro, L N; Jarazo, J; Buemo, C; Hiriart, M I; Sestelo, A; Salamone, D F

    2015-10-01

    The aim of this study was to evaluate three different cloning strategies in the domestic cat (Felis silvestris) and to use the most efficient to generate wild felid embryos by interspecific cloning (iSCNT) using Bengal (a hybrid formed by the cross of Felis silvestris and Prionailurus bengalensis) and tiger (Panthera tigris) donor cells. In experiment 1, zona-free (ZP-free) cloning resulted in higher fusion and expanded blastocyst rates with respect to zona included cloning techniques that involved fusion or injection of the donor cell. In experiment 2, ZP-free iSCNT and embryo aggregation (2X) were assessed. Division velocity and blastocyst rates were increased by embryo aggregation in the three species. Despite fewer tiger embryos than Bengal and cat embryos reached the blastocyst stage, Tiger 2X group increased the percentage of blastocysts with respect to Tiger 1X group (3.2% vs 12.1%, respectively). Moreover, blastocyst cell number was almost duplicated in aggregated embryos with respect to non-aggregated ones within Bengal and tiger groups (278.3 ± 61.9 vs 516.8 ± 103.6 for Bengal 1X and Bengal 2X groups, respectively; 41 vs 220 ± 60 for Tiger 1X and Tiger 2X groups, respectively). OCT4 analysis also revealed that tiger blastocysts had higher proportion of OCT4-positive cells with respect to Bengal blastocysts and cat intracytoplasmic sperm injection blastocysts. In conclusion, ZP-free cloning has improved the quality of cat embryos with respect to the other cloning techniques evaluated and was successfully applied in iSCNT complemented with embryo aggregation. © 2015 Blackwell Verlag GmbH.

  9. Pitch Variability in Patients with Parkinson's Disease: Effects of Deep Brain Stimulation of Caudal Zona Incerta and Subthalamic Nucleus

    ERIC Educational Resources Information Center

    Karlsson, Fredrik; Olofsson, Katarina; Blomstedt, Patric; Linder, Jan; van Doorn, Jan

    2013-01-01

    Purpose: The purpose of the present study was to examine the effect of deep brain stimulation (DBS) of the subthalamic nucleus (STN) and the caudal zona incerta (cZi) pitch characteristics of connected speech in patients with Parkinson's disease (PD). Method: The authors evaluated 16 patients preoperatively and 12 months after DBS surgery. Eight…

  10. INDUCTION OF ZONA RADIATA PROTEINS AND VITELLOGENINS IN ESTRADIOL AND NONYLPHENOL EXPOSED MALE SHEEPSHEAD MINNOWS (CYPRINODON VARIEGATUS)

    EPA Science Inventory

    Knoebl, Iris, Michael J. Hemmer and Nancy D. Denslow. 2004. Induction of Zona Radiata Proteins and Vitellogenins in Estradiol and Nonylphenol Exposed Male Sheepshead Minnows (Cyprinodon variegatus). Mar. Environ. Res. 58(2-5):547-551. (ERL,GB X1059).

    Several genes normall...

  11. The pattern of tyrosine phosphorylation in human sperm in response to binding to zona pellucida or hyaluronic acid.

    PubMed

    Sati, Leyla; Cayli, Sevil; Delpiano, Elena; Sakkas, Denny; Huszar, Gabor

    2014-05-01

    In mammalian species, acquisition of sperm fertilization competence is dependent on the phenomenon of sperm capacitation. One of the key elements of capacitation is protein tyrosine phosphorylation (TP) in various sperm membrane regions. In previous studies performed, the pattern of TP was examined in human sperm bound to zona pellucida of oocytes. In the present comparative study, TP patterns upon sperm binding to the zona pellucida or hyaluronic acid (HA) were investigated in spermatozoa arising from the same semen samples. Tyrosine phosphorylation, visualized by immunofluorescence, was localized within the acrosomal cap, equatorial head region, neck, and the principal piece. Tyrosine phosphorylation has increased in a time-related manner as capacitation progressed, and the phosphorylation pattern was identical within the principal piece and neck, regardless of the sperm bound to the zona pellucida or HA. Thus, the data demonstrated that the patterns of sperm activation-related TP were similar regardless of the spermatozoa bound to zona pellucida or HA. Further, sperm with incomplete development, as detected by excess cytoplasmic retention, failed to exhibit TP.

  12. The Pattern of Tyrosine Phosphorylation in Human Sperm in Response to Binding to Zona Pellucida or Hyaluronic Acid

    PubMed Central

    Sati, Leyla; Cayli, Sevil; Delpiano, Elena; Sakkas, Denny

    2014-01-01

    In mammalian species, acquisition of sperm fertilization competence is dependent on the phenomenon of sperm capacitation. One of the key elements of capacitation is protein tyrosine phosphorylation (TP) in various sperm membrane regions. In previous studies performed, the pattern of TP was examined in human sperm bound to zona pellucida of oocytes. In the present comparative study, TP patterns upon sperm binding to the zona pellucida or hyaluronic acid (HA) were investigated in spermatozoa arising from the same semen samples. Tyrosine phosphorylation, visualized by immunofluorescence, was localized within the acrosomal cap, equatorial head region, neck, and the principal piece. Tyrosine phosphorylation has increased in a time-related manner as capacitation progressed, and the phosphorylation pattern was identical within the principal piece and neck, regardless of the sperm bound to the zona pellucida or HA. Thus, the data demonstrated that the patterns of sperm activation-related TP were similar regardless of the spermatozoa bound to zona pellucida or HA. Further, sperm with incomplete development, as detected by excess cytoplasmic retention, failed to exhibit TP. PMID:24077441

  13. Indicators of Success in Achieving the El Centro College Goals, 1997-2000.

    ERIC Educational Resources Information Center

    El Centro Coll., Dallas, TX.

    This is a report on indicators of success in achieving community college goals at El Centro College (Texas). The report provides statistics from 1997-2000 and focuses on the progress of nine goals: (1) institutionalizing service beyond expectation--according to student satisfaction surveys, campus changes that have occurred between 1996 and 1999…

  14. Centro TORTUGA's Integrated Research and Professional Development Training for Early Stage Hispanic Students in Puerto Rico

    NASA Astrophysics Data System (ADS)

    Moser, F. C.; Allen, M. R.; Barberena-Arias, M.; Clark, J.; Harris, L.; Maldonado, P. M.; Olivo-Delgado, C.; Pierson, J. J.

    2017-12-01

    Over the last five years our multidisciplinary team explored different undergraduate research and professional development (PD) strategies to improve early stage Hispanic student retention in marine science with the objective of interesting them in pursuing degrees that may ultimately lead to geoscience careers. This research led to the 2016 launch of our current project, Centro TORTUGA (Tropical Oceanography Research Training for Undergraduate Academics). Our overarching goal is to increase the number of underrepresented students from minority serving institutions in geoscience-relevant disciplines and careers. Critical to success is building a program rich in both research and PD. Based on qualitative and quantitative evaluations we found students benefited from PD efforts to increase skills in areas such as: 1) speaking and writing English; 2) science communication; 3) teamwork; 4) project management; and 5) completing internship/graduate school applications. To build student self-confidence, networking, and science skills Centro Tortuga involves students' families, bridges cultural gaps across research and non-research institutions inside and outside of Puerto Rico, and provides a gathering place (Centro TORTUGA) for students. With our partners, Universidad del Turabo (UT), Universidad Metropolitana (UMET), and University of Maryland Center for Environmental Sciences, we are now testing a 12-month integrated research and PD curriculum. Initial results suggest areas for improved student training include: 1) science communication (reports and graphs); 2) science ethics; and 3) poster and oral presentations. Students also identified specific preparation they would like included in the Centro TORTUGA curriculum.

  15. Checklist of Mosquito Species (Diptera: Culicidae) in the Rio Grande do Norte State, Brazil-Contribution of Entomological Surveillance.

    PubMed

    Lázaro Silva Inácio, Cássio; Hilário Tavares da Silva, José; César de Melo Freire, Renato; Antonaci Gama, Renata; Brisola Marcondes, Carlos; de Fátima Freire de Melo Ximenes, Maria

    2017-05-01

    The distribution of mosquito species in Rio Grande do Norte state, Brazil, was compiled from published data mid-2016 and a review of specimens deposited in the entomological collection of the Entomology Laboratory of the Federal University of Rio Grande do Norte. The existing records exist for 40 of the 167 municipalities in the state. The specimens in the Entomology Laboratory were collected using Shannon traps and by active search for immature individuals and from aquatic habitats using standard methods, in preserved Atlantic Forest and Caatinga remnants, located in urban and rural areas of the state. In total were recorded 76 species distributed into 25 subgenera, 15 genera, nine tribes, and two subfamilies, in addition to 15 new species records for the state. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  16. The Ilocos Norte Communities against Rabies Exposure Elimination Project in the Philippines: Epidemiological and Economic Aspects.

    PubMed

    Valenzuela, Loida M; Jayme, Sarah I; Amparo, Anna Charinna B; Taylor, Louise H; Dela Cruz, Maria Pinky Z; Licuan, Dianne A; Gamal-Bitao, Rosebelle; Nel, Louis H

    2017-01-01

    As canine rabies control in Africa and Asia transitions from research-led proof-of-concept studies to government-led programs for elimination, experience and evidence of their impact and costs must be shared for the benefit of future programs. The Ilocos Norte Communities against Rabies Exposure project was implemented in April 2012 by the provincial veterinary and health offices and supported by many other partners. It delivered a comprehensive dog vaccination program and increased awareness of the need for postexposure prophylaxis (PEP), aiming to eliminate human and animal rabies cases from Ilocos Norte by 2015. Prior to the intervention, confirmed rabies cases in dogs were between 19 and 50 per year (2008-2011). The primary outcome of the project was a reduction in rabies cases in both dogs and humans to 0 in 2014 and 2015, which has subsequently been maintained. Animal bite consultations increased significantly during the project. Economic data for the dog vaccination and PEP components of the project were collated for two sites: Laoag City (an urban setting) and Dingras Municipality (a rural setting) between 2012 and 2014. The average programmatic cost of vaccinating each dog was $4.54 in Laoag City and $8.65 in Dingras, and costs fell as the project reached more dogs. The average costs of providing PEP were $69.72 per patient and $49.02 per patient for the two sites, respectively, again falling as the project reached more people. External donor contributions contributed less than 20% of dog vaccination costs and less than 1% of PEP costs. The project demonstrated that rabies elimination can be achieved in a short period of time, with concerted effort across multiple sectors. A lack of clear dog population estimates hampered interpretation of some aspects of the programme. From 2016, the provincial government has assumed complete responsibility for the programme and must now continue the vaccination and surveillance efforts. Although safeguards are in place

  17. Intracellular activation of ovastacin mediates pre-fertilization hardening of the zona pellucida.

    PubMed

    Körschgen, Hagen; Kuske, Michael; Karmilin, Konstantin; Yiallouros, Irene; Balbach, Melanie; Floehr, Julia; Wachten, Dagmar; Jahnen-Dechent, Willi; Stöcker, Walter

    2017-09-01

    How and where is pro-ovastacin activated and how does active ovastacin regulate zona pellucida hardening (ZPH) and successful fertilization? Ovastacin is partially active before exocytosis and pre-hardens the zona pellucida (ZP) before fertilization. The metalloproteinase ovastacin is stored in cortical granules, it cleaves zona pellucida protein 2 (ZP2) upon fertilization and thereby destroys the ZP sperm ligand and triggers ZPH. Female mice deficient in the extracellular circulating ovastacin-inhibitor fetuin-B are infertile due to pre-mature ZPH. We isolated oocytes from wild-type and ovastacin-deficient (Astlnull) FVB mice before and after fertilization (in vitro and in vivo) and quantified ovastacin activity and cleavage of ZP2 by immunoblot. We assessed ZPH by measuring ZP digestion time using α-chymotrypsin and by determining ZP2 cleavage. We determined cellular distribution of ovastacin by immunofluorescence using domain-specific ovastacin antibodies. Experiments were performed at least in triplicate with a minimum of 20 oocytes. Data were pre-analyzed using Shapiro-Wilk test. In case of normal distribution, significance was determined via two-sided Student's t-test, whereas in case of non-normal distribution via Mann-Whitney U-test. Metaphase II (MII) oocytes contained both inactive pro-ovastacin and activated ovastacin. Immunoblot and ZP digestion assays revealed a partial cleavage of ZP2 even before fertilization in wild-type mice. Partial cleavage coincided with germinal-vesicle breakdown and MII, despite the presence of fetuin-B protein, an endogenous ovastacin inhibitor, in the follicular and oviductal fluid. Upon exocytosis, part of the C-terminal domain of ovastacin remained attached to the plasmalemma, while the N-terminal active ovastacin domain was secreted. This finding may resolve previously conflicting data showing that ovastacin acts both as an oolemmal receptor termed SAS1B (sperm acrosomal SLLP1 binding protein; SLLP, sperm lysozyme like

  18. Multidrug-resistant pulmonary tuberculosis in Los Altos, Selva and Norte regions, Chiapas, Mexico.

    PubMed

    Sánchez-Pérez, H J; Díaz-Vázquez, A; Nájera-Ortiz, J C; Balandrano, S; Martín-Mateo, M

    2010-01-01

    To analyse the proportion of multidrug-resistant tuberculosis (MDR-TB) in cultures performed during the period 2000-2002 in Los Altos, Selva and Norte regions, Chiapas, Mexico, and to analyse MDR-TB in terms of clinical and sociodemographic indicators. Cross-sectional study of patients with pulmonary tuberculosis (PTB) from the above regions. Drug susceptibility testing results from two research projects were analysed, as were those of routine sputum samples sent in by health personnel for processing (n = 114). MDR-TB was analysed in terms of the various variables of interest using bivariate tests of association and logistic regression. The proportion of primary MDR-TB was 4.6% (2 of 43), that of secondary MDR-TB was 29.2% (7/24), while among those whose history of treatment was unknown the proportion was 14.3% (3/21). According to the logistic regression model, the variables most highly associated with MDR-TB were as follows: having received anti-tuberculosis treatment previously, cough of >3 years' duration and not being indigenous. The high proportion of MDR cases found in the regions studied shows that it is necessary to significantly improve the control and surveillance of PTB.

  19. Dating the Late Archaic occupation of the Norte Chico region in Peru.

    PubMed

    Haas, Jonathan; Creamer, Winifred; Ruiz, Alvaro

    2004-12-23

    The Norte Chico region on the coast of Peru north of Lima consists of four adjacent river valleys--Huaura, Supe, Pativilca and Fortaleza--in which archaeologists have been aware of a number of apparently early sites for more than 40 years (refs 1- 3). To clarify the early chronology in this region, we undertook fieldwork in 2002 and 2003 to determine the dates of occupation of sites in the Fortaleza and Pativilca valleys. Here we present 95 new radiocarbon dates from a sample of 13 of more than 20 large, early sites. These sites share certain basic characteristics, including large-scale monumental architecture, extensive residential architecture and a lack of ceramics. The 95 new dates confirm the emergence and development of a major cultural complex in this region during the Late Archaic period between 3000 and 1800 calibrated calendar years bc. The results help to redefine a broader understanding of the respective roles of agricultural and fishing economies in the beginnings of civilization in South America.

  20. Albedo estimation using near infrared photography at Glaciar Norte of Citlaltepetl Volcano (Mexico).

    NASA Astrophysics Data System (ADS)

    Ontiveros, Guillermo; Delgado-Granados, Hugo

    2015-04-01

    In this work we show preliminary results of the application of the methodology proposed by Corripio (2004) for albedo estimation of a glacial surface using oblique photography. This analysis was performed for the Glaciar Norte of Citlaltepetl volcano (Mexico), using images obtained with a modified digital camera for capturing the portion of the near infrared spectrum starting at 950 nm and a digital elevation model with a grid of 2 m. The main goal was to obtain a picture of the spatial distribution of albedo on the glacier, in order to find out if there was any morphological evidence of the influence of the glacier energy balance. Some of the obtained results show a certain spatial distribution with comparatively higher albedo values at the lower parts of the glacier as compared with higher parts. The higher values may correspond to different metamorphism of snow/ice at different heights due to the effects of lower slope. Corripio, J. G. (2004). Snow surface albedo estimation using terrestrial photography. International journal of remote sensing, 25(24), 5705-5729.

  1. Mapping the potential beverage quality of coffee produced in the Zona da Mata, Minas Gerais, Brazil.

    PubMed

    Silva, Samuel de Assis; de Queiroz, Daniel Marçal; Ferreira, Williams Pinto Marques; Corrêa, Paulo Cesar; Rufino, José Luis Dos Santos

    2016-07-01

    Detailed knowledge of coffee production systems enables optimization of crop management, harvesting and post-harvest techniques. In this study, coffee quality is mapped as a function of coffee variety, altitude and terrain aspect attributes. The work was performed in the Zona da Mata, Minas Gerais, Brazil. A large range of coffee quality grades was observed for the Red Catuai variety. For the Yellow Catuai variety, no quality grades lower than 70 were observed. Regarding the terrain aspect, samples from the southeast-facing slope (SEFS) and the northwest-facing slope (NWFS) exhibited distinct behaviors. The SEFS samples had a greater range of quality grades than did the NWFS samples. The highest grade was obtained from an NWFS point. The lowest quality values and the largest range of grades were observed at lower altitudes. The extracts from the highest-altitude samples did not produce any low-quality coffee. The production site's position and altitude are the primary variables that influenced the coffee quality. The study area has micro-regions with grades ranging from 80 to 94. These areas have the potential for producing specialty coffees. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  2. Population genetic structure of Attalea vitrivir Zona (Arecaceae) in fragmented areas of southeast Brazil.

    PubMed

    Santos, R R M; Cavallari, M M; Pimenta, M A S; Abreu, A G; Costa, M R; Guedes, M L

    2015-06-11

    Attalea vitrivir Zona (synonym Orbignya oleifera) is one of the six species of Arecaceae known as "babassu". This species is used to make cosmetics, food, and detergents due to the high concentration of oil in the seeds. It is found only in fragmented areas of southern Bahia State and northern Minas Gerais State, southeast Brazil, and this fragmentation has affected both its ecological and genetic characteristics. We evaluated the genetic diversity and population genetic structure of A. vitrivir in six areas of two different regions at the extremes of its geographical range, in order to gain a better understanding of the factors that affect the distribution and partitioning of its diversity. Nine inter simple sequence repeat (ISSR) markers amplified 74 polymorphic bands, resulting in large diversity values (Shannon diversity index, 0.37-0.47; intrapopulation genetic diversity, 0.25-0.34). Analysis of molecular variance (AMOVA) revealed considerable differentiation between sampling sites (30.03%) and regions (12.08%), although most of the diversity was observed within sampling sites (69%). Further differentiation between sampling sites was noted more in the northern region than in the southern region, highlighting the genetic connectivity between the sampling sites within Rio Pandeiros Environmental Protection Area (southern region). The identification of two distinct genetic clusters (K = 2) corresponded to the northern and southern regions, and corroborated the AMOVA results. We suggest that the northern area, outside Rio Pandeiros Environmental Protection Area, must be included in future management plans for this species.

  3. Estimating Young's modulus of zona pellucida by micropipette aspiration in combination with theoretical models of ovum

    PubMed Central

    Khalilian, Morteza; Navidbakhsh, Mahdi; Valojerdi, Mojtaba Rezazadeh; Chizari, Mahmoud; Yazdi, Poopak Eftekhari

    2010-01-01

    The zona pellucida (ZP) is the spherical layer that surrounds the mammalian oocyte. The physical hardness of this layer plays a crucial role in fertilization and is largely unknown because of the lack of appropriate measuring and modelling methods. The aim of this study is to measure the biomechanical properties of the ZP of human/mouse ovum and to test the hypothesis that Young's modulus of the ZP varies with fertilization. Young's moduli of ZP are determined before and after fertilization by using the micropipette aspiration technique, coupled with theoretical models of the oocyte as an elastic incompressible half-space (half-space model), an elastic compressible bilayer (layered model) or an elastic compressible shell (shell model). Comparison of the models shows that incorporation of the layered geometry of the ovum and the compressibility of the ZP in the layered and shell models may provide a means of more accurately characterizing ZP elasticity. Evaluation of results shows that although the results of the models are different, all confirm that the hardening of ZP will increase following fertilization. As can be seen, different choices of models and experimental parameters can affect the interpretation of experimental data and lead to differing mechanical properties. PMID:19828504

  4. Detection of angiotensin II binding to single adrenal zona glomerulosa cells by confocal Raman microspectroscopy

    NASA Astrophysics Data System (ADS)

    McCoy, Michael J.; Habermann, Timothy J.; Hanke, Craig J.; Adar, Fran; Campbell, William B.; Nithipatikom, Kasem

    1999-04-01

    We developed a confocal Raman microspectroscopic technique to study ligand-receptor bindings in single cells using Raman-labeled ligands and surface-enhanced Raman scattering (SERS). The adrenal zona glomerulosa (ZG) cells were used as a model in this study. ZG cells have a high density of angiotensin II (AII) receptors on the cellular membrane. There are two identified subtypes of AII receptors,namely AT1 and AT2 receptors. AII is a peptidic hormone, which upon binding to its receptors, stimulates the release of aldosterone from ZG cells. The cellular localization of these receptors subtypes was detected in single ZG cells by using immunocomplexation of receptors with specific antibodies and confocal Raman microspectroscopy. In the binding study, we used biotin-labeled AII to bind to its receptors in ZG cells. Then, avidin and Raman-labeled AII. The binding was measure directly on the single ZG cells. The results showed that the binding was displaced with unlabeled AII and specific AII antagonists. This is a rapid and sensitive technique for detection of cellular ligand bindings as well as antagonists screening in drug discovery.

  5. Capital social de los padres de escolares de una zona vulnerable.

    PubMed

    Cornejo, Susana; Herrera, Ariel; Hilas, Elena; Gigena, Y Pablo

    2018-01-01

    El capital social supone importantes inversiones materiales, simbólicas y de esfuerzos. Conocer el capital social de una comunidad puede facilitar la comprensión del beneficio de las relaciones comunitarias para la promoción de salud. Con el objetivo de reconocer los componentes del capital social de padres de escolares atendiendo una escuela en una zona vulnerable de la ciudad de Córdoba, Argentina, indagamos a través de grupos focales acerca de elementos esenciales del capital social. La asintonía socio-cultural entre los pobladores originarios y los reubicados, la estigmatización policial y el clientelismo político han generado desconfianza del uno hacia el otro en diferentes aspectos convivenciales, siendo la familia la única red de apoyo. La escuela se reconoce como espacio adonde la gente espontáneamente se organiza para invertir en redes sociales, presentándose así con potencialidad para la promoción de conductas saludables, por el lugar simbólico que ocupa para los padres.

  6. Phosphorylation of zona occludens-2 by protein kinase C epsilon regulates its nuclear exportation.

    PubMed

    Chamorro, David; Alarcón, Lourdes; Ponce, Arturo; Tapia, Rocio; González-Aguilar, Héctor; Robles-Flores, Martha; Mejía-Castillo, Teresa; Segovia, José; Bandala, Yamir; Juaristi, Eusebio; González-Mariscal, Lorenza

    2009-09-01

    Here, we have analyzed the subcellular destiny of newly synthesized tight junction protein zona occludens (ZO)-2. After transfection in sparse cells, 74% of cells exhibit ZO-2 at the nucleus, and after 18 h the value decreases to 17%. The mutation S369A located within the nuclear exportation signal 1 of ZO-2 impairs the nuclear export of the protein. Because Ser369 represents a putative protein kinase C (PKC) phosphorylation site, we tested the effect of PKC inhibition and stimulation on the nuclear export of ZO-2. Our results strongly suggest that the departure of ZO-2 from the nucleus is regulated by phosphorylation at Ser369 by novel PKCepsilon. To test the route taken by ZO-2 from synthesis to the plasma membrane, we devised a novel nuclear microinjection assay in which the nucleus served as a reservoir for anti-ZO-2 antibody. Through this assay, we demonstrate that a significant amount of newly synthesized ZO-2 goes into the nucleus and is later relocated to the plasma membrane. These results constitute novel information for understanding the mechanisms that regulate the intracellular fate of ZO-2.

  7. Demi-embryo production from hatching of zona-drilled bovine and rabbit blastocysts.

    PubMed

    Skrzyszowska, M; Smorag, Z; Katska, L

    1997-09-01

    It is known that the pregnancy rate resulting after transfer of bisected embryos is lower than after transfer of whole embryos. The main reason is the reduced cell number in the demi-embryo which is less than 1 2 of that in the intact embryo, since a number of blastomeres is damaged as a result of the procedure used in conventional embryo splitting. The aim of our experiment was to develop a non-invasive procedure which would limit cell losses during microsurgery. The experiment was carried out on bovine IVM-IVF embryos at middle, late and expanded blastocyst stage and rabbit embryos at late blastocyst stage cultured in vitro from in vivo produced zygotes. The zona pellucida of these embryos was drilled on the line between the inner cell mass and the trophoblast using a glass microneedle (

  8. A Flexure-Guided Piezo Drill for Penetrating the Zona Pellucida of Mammalian Oocytes.

    PubMed

    Johnson, Wesley; Dai, Changsheng; Liu, Jun; Wang, Xian; Luu, Devin K; Zhang, Zhuoran; Ru, Changhai; Zhou, Chao; Tan, Min; Pu, Huayan; Xie, Shaorong; Peng, Yan; Luo, Jun; Sun, Yu

    2018-03-01

    Mammalian oocytes such as mouse oocytes have a highly elastic outer membrane, zona pellucida (ZP) that cannot be penetrated without significantly deforming the oocyte, even with a sharp micropipette. Piezo drill devices leverage lateral and axial vibration of the micropipette to accomplish ZP penetration with greatly reduced oocyte deformation. However, existing piezo drills all rely on a large lateral micropipette vibration amplitude ( 20 ) and a small axial vibration amplitude (0.1 ). The very large lateral vibration amplitude has been deemed to be necessary for ZP penetration although it also induces larger oocyte deformation and more oocyte damage. This paper reports on a new piezo drill device that uses a flexure guidance mechanism and a systematically designed pulse train with an appropriate base frequency. Both simulation and experimental results demonstrate that a small lateral vibration amplitude (e.g., 2 ) and an axial vibration amplitude as large as 1.2 were achieved. Besides achieving 100% effectiveness in the penetration of mouse oocytes (n = 45), the new piezo device during ZP penetration induced a small oocyte deformation of 3.4 versus larger than 10 using existing piezo drill devices.

  9. Analysis of ZP1 gene reveals differences in zona pellucida composition in carnivores.

    PubMed

    Moros-Nicolás, C; Leza, A; Chevret, P; Guillén-Martínez, A; González-Brusi, L; Boué, F; Lopez-Bejar, M; Ballesta, J; Avilés, M; Izquierdo-Rico, M J

    2018-01-01

    The zona pellucida (ZP) is an extracellular envelope that surrounds mammalian oocytes. This coat participates in the interaction between gametes, induction of the acrosome reaction, block of polyspermy and protection of the oviductal embryo. Previous studies suggested that carnivore ZP was formed by three glycoproteins (ZP2, ZP3 and ZP4), with ZP1 being a pseudogene. However, a recent study in the cat found that all four proteins were expressed. In the present study, in silico and molecular analyses were performed in several carnivores to clarify the ZP composition in this order of mammals. The in silico analysis demonstrated the presence of the ZP1 gene in five carnivores: cheetah, panda, polar bear, tiger and walrus, whereas in the Antarctic fur seal and the Weddell seal there was evidence of pseudogenisation. Molecular analysis showed the presence of four ZP transcripts in ferret ovaries (ZP1, ZP2, ZP3 and ZP4) and three in fox ovaries (ZP2, ZP3 and ZP4). Analysis of the fox ZP1 gene showed the presence of a stop codon. The results strongly suggest that all four ZP genes are expressed in most carnivores, whereas ZP1 pseudogenisation seems to have independently affected three families (Canidae, Otariidae and Phocidae) of the carnivore tree.

  10. Orexin Gene Transfer into Zona Incerta Neurons Suppresses Muscle Paralysis in Narcoleptic Mice

    PubMed Central

    Liu, Meng; Konadhode, RodaRani; Begum, Suraiya; Pelluru, Dheeraj; Gerashchenko, Dmitry; Sakurai, Takeshi; Yanagisawa, Masashi; van den Pol, Anthony N.

    2011-01-01

    Cataplexy, a sudden unexpected muscle paralysis, is a debilitating symptom of the neurodegenerative sleep disorder, narcolepsy. During these attacks, the person is paralyzed, but fully conscious and aware of their surroundings. To identify potential neurons that might serve as surrogate orexin neurons to suppress such attacks, the gene for orexin (hypocretin), a peptide lost in most human narcoleptics, was delivered into the brains of the orexin-ataxin-3 transgenic mouse model of human narcolepsy. Three weeks after the recombinant adenoassociated virus (rAAV)-mediated orexin gene transfer, sleep–wake behavior was assessed. rAAV-orexin gene delivery into neurons of the zona incerta (ZI), or the lateral hypothalamus (LH) blocked cataplexy. Orexin gene transfer into the striatum or in the melanin-concentrating hormone neurons in the ZI or LH had no such effect, indicating site specificity. In transgenic mice lacking orexin neurons but given rAAV-orexin, detectable levels of orexin-A were evident in the CSF, indicating release of the peptide from the surrogate neurons. Retrograde tracer studies showed that the amygdala innervates the ZI consistent with evidence that strong emotions trigger cataplexy. In turn, the ZI projects to the locus ceruleus, indicating that the ZI is part of a circuit that stabilizes motor tone. Our results indicate that these neurons might also be recruited to block the muscle paralysis in narcolepsy. PMID:21508228

  11. Glycoprotein from the liver constitutes the inner layer of the egg envelope (zona pellucida interna) of the fish, Oryzias latipes

    SciTech Connect

    Hamazaki, T.S.; Nagahama, Y.; Iuchi, I.

    1989-05-01

    A glycoprotein from the liver, which shares epitopes with chorion (egg envelope or zona pellucida) glycoproteins, is present only in the spawning female fish, Oryzias latipes, under natural conditions. This spawning female-specific (SF) substance is distinct from vitellogenin but closely resembles a major glycoprotein component, ZI-3, of the inner layer (zona radiata interna) of the ovarian egg envelope with respect to some biochemical and immunochemical characteristics. Here we report that the (/sup 125/I)SF substance, injected into the abdominal cavity of the spawning female fish, was rapidly transported by the blood circulation into the ovary and incorporated into the inner layermore » of egg envelope of the growing oocytes. The result strongly suggests that the SF substance from the liver is a precursor substance of the major component, ZI-3, of the inner layer of egg envelope in the fish.« less

  12. Development and Dissemination of the El Centro Health Disparities Measures Library.

    PubMed

    Mitrani, Victoria Behar; O'Day, Joanne E; Norris, Timothy B; Adebayo, Oluwamuyiwa Winifred

    2017-09-01

    This report describes the development and dissemination of a library of English measures, with Spanish translations, on constructs relevant to social determinants of health and behavioral health outcomes. The El Centro Measures Library is a product of the Center of Excellence for Health Disparities Research: El Centro, a program funded by the National Institute on Minority Health and Health Disparities of the U.S. National Institutes of Health. The library is aimed at enhancing capacity for minority health and health disparities research, particularly for Hispanics living in the United States and abroad. The open-access library of measures (available through www.miami.edu/sonhs/measureslibrary) contains brief descriptions of each measure, scoring information (where available), links to related peer-reviewed articles, and measure items in both languages. Links to measure websites where commercially available measures can be purchased are included, as is contact information for measures that require author permission. Links to several other measures libraries are hosted on the library website. Other researchers may contribute to the library. El Centro investigators began the library by electing to use a common set of measures across studies to assess demographic information, culture-related variables, proximal outcomes of interest, and major outcomes. The collection was expanded to include other health disparity research studies. In 2012, a formal process was developed to organize, expand, and centralize the library in preparation for a gradual process of dissemination to the national and international community of researchers. The library currently contains 61 measures encompassing 12 categories of constructs. Thus far, the library has been accessed 8,883 times (unique page views as generated by Google Analytics), and responses from constituencies of users and measure authors have been favorable. With the paucity of availability and accessibility of translated

  13. Oocytes with a dark zona pellucida demonstrate lower fertilization, implantation and clinical pregnancy rates in IVF/ICSI cycles.

    PubMed

    Shi, Wei; Xu, Bo; Wu, Li-Min; Jin, Ren-Tao; Luan, Hong-Bing; Luo, Li-Hua; Zhu, Qing; Johansson, Lars; Liu, Yu-Sheng; Tong, Xian-Hong

    2014-01-01

    The morphological assessment of oocytes is important for embryologists to identify and select MII oocytes in IVF/ICSI cycles. Dysmorphism of oocytes decreases viability and the developmental potential of oocytes as well as the clinical pregnancy rate. Several reports have suggested that oocytes with a dark zona pellucida (DZP) correlate with the outcome of IVF treatment. However, the effect of DZP on oocyte quality, fertilization, implantation, and pregnancy outcome were not investigated in detail. In this study, a retrospective analysis was performed in 268 infertile patients with fallopian tube obstruction and/or male factor infertility. In 204 of these patients, all oocytes were surrounded by a normal zona pellucida (NZP, control group), whereas 46 patients were found to have part of their retrieved oocytes enclosed by NZP and the other by DZP (Group A). In addition, all oocytes enclosed by DZP were retrieved from 18 patients (Group B). No differences were detected between the control and group A. Compared to the control group, the rates of fertilization, good quality embryos, implantation and clinical pregnancy were significantly decreased in group B. Furthermore, mitochondria in oocytes with a DZP in both of the two study groups (A and B) were severely damaged with several ultrastructural alterations, which were associated with an increased density of the zona pellucida and vacuolization. Briefly, oocytes with a DZP affected the clinical outcome in IVF/ICSI cycles and appeared to contain more ultrastructural alterations. Thus, DZP could be used as a potential selective marker for embryologists during daily laboratory work.

  14. Sperm Mitochondrial Integrity Is Not Required for Hyperactivated Motility, Zona Binding, or Acrosome Reaction in the Rhesus Macaque1

    PubMed Central

    Hung, Pei-hsuan; Miller, Marion G.; Meyers, Stuart A.; VandeVoort, Catherine A.

    2008-01-01

    Whether the main energy source for sperm motility is from oxidative phosphorylation or glycolysis has been long-debated in the field of reproductive biology. Using the rhesus monkey as a model, we examined the role of glycolysis and oxidative phosphorylation in sperm function by using alpha-chlorohydrin (ACH), a glycolysis inhibitor, and pentachlorophenol (PCP), an oxidative phosphorylation uncoupler. Sperm treated with ACH showed no change in percentage of motile sperm, although sperm motion was impaired. The ACH-treated sperm did not display either hyperactivity- or hyperactivation-associated changes in protein tyrosine phosphorylation. When treated with PCP, sperm motion parameters were affected by the highest level of PCP (200 μM); however, PCP did not cause motility impairments even after chemical activation. Sperm treated with PCP were able to display hyperactivity and tyrosine phosphorylation after chemical activation. In contrast with motility measurements, treatment with either the glycolytic inhibitor or the oxidative phosphorylation inhibitor did not affect sperm-zona binding and zona-induced acrosome reaction. The results suggest glycolysis is essential to support sperm motility, hyperactivity, and protein tyrosine phosphorylation, while energy from oxidative phosphorylation is not necessary for hyperactivated sperm motility, tyrosine phosphorylation, sperm-zona binding, and acrosome reaction in the rhesus macaque. PMID:18480469

  15. Lithologic controls on mineralization at the Lagunas Norte high-sulfidation epithermal gold deposit, northern Peru

    NASA Astrophysics Data System (ADS)

    Cerpa, Luis M.; Bissig, Thomas; Kyser, Kurt; McEwan, Craig; Macassi, Arturo; Rios, Hugo W.

    2013-06-01

    The 13.1-Moz high-sulfidation epithermal gold deposit of Lagunas Norte, Alto Chicama District, northern Peru, is hosted in weakly metamorphosed quartzites of the Upper Jurassic to Lower Cretaceous Chimú Formation and in overlying Miocene volcanic rocks of dacitic to rhyolitic composition. The Dafne and Josefa diatremes crosscut the quartzites and are interpreted to be sources of the pyroclastic volcanic rocks. Hydrothermal activity was centered on the diatremes and four hydrothermal stages have been defined, three of which introduced Au ± Ag mineralization. The first hydrothermal stage is restricted to the quartzites of the Chimú Formation and is characterized by silice parda, a tan-colored aggregate of quartz-auriferous pyrite-rutile ± digenite infilling fractures and faults, partially replacing silty beds and forming cement of small hydraulic breccia bodies. The δ34S values for pyrite (1.7-2.2 ‰) and digenite (2.1 ‰) indicate a magmatic source for the sulfur. The second hydrothermal stage resulted in the emplacement of diatremes and the related volcanic rocks. The Dafne diatreme features a relatively impermeable core dominated by milled slate from the Chicama Formation, whereas the Josefa diatreme only contains Chimú Formation quartzite clasts. The third hydrothermal stage introduced the bulk of the mineralization and affected the volcanic rocks, the diatremes, and the Chimú Formation. In the volcanic rocks, classic high-sulfidation epithermal alteration zonation exhibiting vuggy quartz surrounded by a quartz-alunite and a quartz-alunite-kaolinite zone is observed. Company data suggest that gold is present in solid solution or micro inclusions in pyrite. In the quartzite, the alteration is subtle and is manifested by the presence of pyrophyllite or kaolinite in the silty beds, the former resulting from relatively high silica activities in the fluid. In the quartzite, gold mineralization is hosted in a fracture network filled with coarse alunite

  16. Phlebotomine sandflies (Diptera: Psychodidae) in a sub-Andean forest from the Norte de Santander, Colombia.

    PubMed

    Hoyos-López, Richard; Bolaños, Rafael; Contreras-Gutierrez, MarIa; Carrero-Sarmiento, Diego

    2016-03-01

    The recognition of communities of arthropods with medical importance in natural systems constitutes an important step in the prediction of possible epidemic events and/or emergence of infectious diseases in the human population. This is due to anthropogenic impact in natural areas and landscape modification, which changes the dynamics of pathogenic agents, reservoirs, and vector insects. In this study, an inventory was compiled of species of the genus Lutzomyia present in sub-Andean forest from the confluence of the Pamplonita River basin. CDC-light and Shannon traps were used for collecting adult phlebotomine sandflies during the month of October 2013 in a sub-Andean forest from river basin Pamplonita. All specimens were identified using morphological keys. The epidemiological relevance of each species was reported using a literature review about natural infection or vector incrimination with Leishmania species or other pathogens microorganism. A total of 2755 specimens belonging to eight species of the genus Lutzomyia were collected. Out of the eight species, seven belonged to the group verrucarum (Lutzomyia sp--townsendi series, L. ovallesi, L. spinicrassa, L. serrana, L. townsendi, L. nuneztovari and L. pia), while one belonged to the subgenus Helcocyrtomyia (L. hartmanni). A new registry of L. townsendi was observed for the Norte de Santander department. The appreciable diversity of the verrucarum group observed in this area suggest further investigation on the biogeography and evolution of this group, and epidemiological risk for human populations around this area, as there are reports of Leishmania natural infection and favourable conditions for domestication of phlebotomines in rural towns.

  17. Hydrocarbon source apportionment for the 1996 Paso del Norte Ozone Study.

    PubMed

    Fujita, E M

    2001-08-10

    The 1996 Paso del Norte (PdN) ozone study was conducted to improve current understanding of the significant meteorological and air quality processes that lead to high concentrations of ozone in El Paso, Texas (USA) and Ciudad Juárez, Chihuahua (Mexico). Two-hour canister samples were collected five times daily at 05.00-07.00 h, 07.00-09.00 h, 09.00-11.00 h, 11.00-13.00 h, and 15.00-17.00 h MST during intensive study periods at one urban and one rural site on each side of the border. An automated gas chromatograph was operated at one site in central El Paso. Source profiles (the fractional chemical composition of emissions) from motor vehicles, gasoline, liquefied petroleum gas, and commercial natural gas were combined with source profiles from other studies for input to the Chemical Mass Balance (CMB) receptor model to apportion the measured non-methane hydrocarbons (NMHC) to sources. On-road vehicle emissions accounted for one-half to two-thirds of the NMHC in Ciudad Juárez and El Paso with the highest contributions occurring during the morning and afternoon commute periods. Emissions from diesel exhaust contributed approximately 2-3% of NMHC in Ciudad Juárez and less than 2% in El Paso. The average sum of liquid gasoline and gasoline vapor increased during the day in Ciudad Juárez from 2% at 06.00 h to approximately 12% at 16.00 h. Diurnal and day-of-the-week patterns in the liquid gasoline contributions are essentially identical to the corresponding patterns for motor vehicle exhaust, which suggest that a large fraction of the liquid gasoline contribution may be associated with tailpipe emissions rather than evaporative emissions from motor vehicles or industrial sources. Including the sum of the two sources put the upper limit for tailpipe contributions at 60-70% of NMHC.

  18. Modeling the Crust and Upper Mantle in Northern Beata Ridge (CARIBE NORTE Project)

    NASA Astrophysics Data System (ADS)

    Núñez, Diana; Córdoba, Diego; Cotilla, Mario Octavio; Pazos, Antonio

    2016-05-01

    The complex tectonic region of NE Caribbean, where Hispaniola and Puerto Rico are located, is bordered by subduction zone with oblique convergence in the north and by incipient subduction zone associated to Muertos Trough in the south. Central Caribbean basin is characterized by the presence of a prominent topographic structure known as Beata Ridge, whose oceanic crustal thickness is unusual. The northern part of Beata Ridge is colliding with the central part of Hispaniola along a transverse NE alignment, which constitutes a morphostructural limit, thus producing the interruption of the Cibao Valley and the divergence of the rivers and basins in opposite directions. The direction of this alignment coincides with the discontinuity that could explain the extreme difference between west and east seismicity of the island. Different studies have provided information about Beata Ridge, mainly about the shallow structure from MCS data. In this work, CARIBE NORTE (2009) wide-angle seismic data are analyzed along a WNW-ESE trending line in the northern flank of Beata Ridge, providing a complete tectonic view about shallow, middle and deep structures. The results show clear tectonic differences between west and east separated by Beata Island. In the Haiti Basin area, sedimentary cover is strongly influenced by the bathymetry and its thickness decreases toward to the island. In this area, the Upper Mantle reaches 20 km deep increasing up to 24 km below the island where the sedimentary cover disappears. To the east, the three seamounts of Beata Ridge provoke the appearance of a structure completely different where sedimentary cover reaches thicknesses of 4 km between seamounts and Moho rises up to 13 km deep. This study has allowed to determine the Moho topography and to characterize seismically the first upper mantle layers along the northern Beata Ridge, which had not been possible with previous MCS data.

  19. Meteorological Simulations of Ozone Episode Case Days during the 1996 Paso del Norte Ozone Study

    SciTech Connect

    Brown, M.J.; Costigan, K.; Muller, C.

    1999-02-01

    Meteorological simulations centered around the border cities of El Paso and Ciudad Juarez have been performed during an ozone episode that occurred on Aug. 13,1996 during the 1996 Paso del Norte Ozone Study field campaign. Simulations were petiormed using the HOTMAC mesoscale meteorological model using a 1,2,4, and 8 km horizontal grid size nested mesh system. Investigation of the vertical structure and evolution of the atmospheric boundary layer for the Aug. 11-13 time period is emphasized in this paper. Comparison of model-produced wind speed profiles to rawirisonde and radar profiler measurements shows reasonable agreement. A persistent upper-level jet was capturedmore » in the model simulations through data assimilation. In the evening hours, the model was not able to produce the strong wind direction shear seen in the radar wind profiles. Based on virtual potential temperature profile comparisons, the model appears to correctly simulate the daytime growth of the convective mixed layer. However, the model underestimates the cooling of the surface layer at night. We found that the upper-level jet significantly impacted the turbulence structure of the boundary layer, leading to relatively high turbulent kinetic energy (tke) values aloft at night. The model indicates that these high tke values aloft enhance the mid-morning growth of the boundary layer. No upper-level turbulence measurements were available to verify this finding, however. Radar profiler-derived mixing heights do indicate relatively rapid morning growth of the mixed layer.« less

  20. Outcomes from stimulation of the caudal zona incerta and pedunculopontine nucleus in patients with Parkinson's disease.

    PubMed

    Khan, Sadaquate; Mooney, Lucy; Plaha, Puneet; Javed, Shazia; White, Paul; Whone, Alan L; Gill, Steven S

    2011-04-01

    Axial symptoms including postural instability, falls and failure of gait initiation are some of the most disabling motor symptoms of Parkinson's disease (PD). We performed bilateral deep brain stimulation (DBS) of the pedunculopontine nucleus (PPN) in combination with the caudal zona incerta (cZi) in order to determine their efficacy in alleviating these symptoms. Seven patients with predominant axial symptoms in both the 'on' and 'off' medication states underwent bilateral cZi and PPN DBS. Motor outcomes were assessed using the motor component of the Unified Parkinson's Disease Rating Scale (UPDRS 3) and a composite axial subscore was derived from items 27, 28, 29 and 30 (arising from chair, posture, gait and postural stability). Quality of life was measured using the PDQ39. Comparisons were made between scores obtained at baseline and those at a mean follow-up of 12 months. In both the off and on medication states, a statistically significant improvement in the UPDRS part 3 score was achieved by stimulation of the PPN, cZi and both in combination. In the off medication state, our composite axial subscore of the UPDRS part 3 improved with stimulation of the PPN, cZi and both in combination. The composite axial subscore, in the 'on' medication state, however, only showed a statistically significant improvement when a combination of cZi and PPN stimulation was used. This study provides evidence that a combination of PPN and cZi stimulation can achieve a significant improvement in the hitherto untreatable 'on' medication axial symptoms of PD.

  1. Vitrification of zona-free rabbit expanded or hatching blastocysts: a possible model for human blastocysts.

    PubMed

    Cervera, R P; Garcia-Ximénez, F

    2003-10-01

    The purpose of this study was to test the effectiveness of one two-step (A) and two one-step (B1 and B2) vitrification procedures on denuded expanded or hatching rabbit blastocysts held in standard sealed plastic straws as a possible model for human blastocysts. The effect of blastocyst size was also studied on the basis of three size categories (I: diameter <200 micro m; II: diameter 200-299 micro m; III: diameter >/==" BORDER="0">300 micro m). Rabbit expanded or hatching blastocysts were vitrified at day 4 or 5. Before vitrification, the zona pellucida was removed using acidic phosphate buffered saline. For the two-step procedure, prior to vitrification, blastocysts were pre- equilibrated in a solution containing 10% dimethyl sulphoxide (DMSO) and 10% ethylene glycol (EG) for 1 min. Different final vitrification solutions were compared: 20% DMSO and 20% EG with (A and B1) or without (B2) 0.5 mol/l sucrose. Of 198 vitrified blastocysts, 181 (91%) survived, regardless of the vitrification procedure applied. Vitrification procedure A showed significantly higher re-expansion (88%), attachment (86%) and trophectoderm outgrowth (80%) rates than the two one-step vitrification procedures, B1 and B2 (46 and 21%, 20 and 33%, and 18 and 23%, respectively). After warming, blastocysts of greater size (II and III) showed significantly higher attachment (54 and 64%) and trophectoderm outgrowth (44 and 58%) rates than smaller blastocysts (I, attachment: 29%; trophectoderm outgrowth: 25%). These result demonstrate that denuded expanded or hatching rabbit blastocysts of greater size can be satisfactorily vitrified by use of a two-step procedure. The similarity of vitrification solutions used in humans could make it feasible to test such a procedure on human denuded blastocysts of different sizes.

  2. Foaling rates in feral horses treated with the immunocontraceptive porcine zona pellucida

    USGS Publications Warehouse

    Ransom, J.I.; Roelle, J.E.; Cade, B.S.; Coates-Markle, L.; Kane, A.J.

    2011-01-01

    Locally abundant feral horses (Equus caballus) can rapidly deplete available resources. Fertility control agents present promising nonlethal tools for reducing their population growth rates. We tested the effect of 2 forms of the immunocontraceptive porcine zona pellucida (PZP) on foaling rates in 3 populations of feral horses in the western United States. A liquid form requiring annual boosters was administered at Little Book Cliffs Wild Horse Range, Mesa County (CO), and Pryor Mountain Wild Horse Range, Bighorn County (WY) and Carbon County (MT), and a time-release pellet form designed to produce 2 yr of infertility was administered at McCullough Peaks Herd Management Area, Park County (WY). Average foaling rates (foals born/mare-yr) from direct observation of untreated and treated female horses (mares), 2004-2008, were 60.1% (n = 153 mare-yr) versus 6.6% (n = 91 mare-yr) at Little Book Cliffs, and 62.8% (n = 129 mare-yr) versus 17.7% (n = 79 mare-yr) at Pryor Mountain, respectively. At McCullough Peaks, mean annual foaling rates from 2006 to 2008 were 75.0% (n = 48 mare-yr) for untreated mares and 31.7% (n = 101 mare-yr) for treated mares. Controlling for age of mares and pretreatment differences in fertility, PZP reduced foaling rates in all 3 herds. The pellets used at McCullough Peaks (produced by cold evaporation) were less effective than pellets used in a previous trial and produced by heat extrusion. Immunocontraception with PZP may be a useful tool in reducing fertility rates in some western United States feral horse herds, but population growth reduction will depend on timely access to mares for inoculation and the proportion of mares that can be successfully treated. ?? 2011 The Wildlife Society.

  3. [Molecules involved in sperm-zona pellucida interaction in mammals. Role in human fertility].

    PubMed

    Serres, Catherine; Auer, Jana; Petit, François; Patrat, Catherine; Jouannet, Pierre

    2008-01-01

    Fertilization in mammals requires an initial interaction of sperm with the oocyte envelope, the zona pellucida (ZP), before it reaches the oocyte. ZP is a highly glycosylated structure, composed of three (mouse) or four (rabbit, boar, bovine, humans...) glycoproteins. The presence of ZP around the oocyte does not allow heterospecific fertilization. This barrier is principally due to the presence of species-specific glycosylations on ZP proteins. Sperm bind ZP by means of membrane receptors which recognize carbohydrate moieties on ZP glycoproteins according to a well-precised sequential process. Upon initial attachment, spermatozoa bind ZP3/ZP4 which induces the sperm acrosome exocytosis followed by a secondary binding of acrosome reacted spermatozoa to ZP2 and by ZP penetration. The sperm receptors are adhesive proteins or integral plasma membrane proteins linked to intraspermatic signalling pathways activating the acrosome reaction. Over the last twenty years, numerous studies have been carried out to identify sperm receptors to ZP in several species, but the data in humans are still incomplete. Work initiated in our research group has identified several proteins interacting with recombinant human ZP2, ZP3 and ZP4, among which are glycolytic enzymes. These enzymes are involved in the gamete interaction by means of their affinity to sugars and not by their catalytic properties. From a clinical point of view, an observed lack or weak expression of some sperm receptors to ZP3 in cases of idiopathic infertility associated with in vitro fertilization failure suggests that knowing the molecular mechanism driving the gamete recognition can be important at the diagnostic level. Furthermore, it has been shown that proteins that mediate gamete recognition diverge rapidly, as a result of positive darwinian selection. A sexual conflict can drive co-evolution of reproductive molecules in both sexes resulting in reproductive isolation and species emergence.

  4. Effects of acrolein on aldosterone release from zona glomerulosa cells in male rats.

    PubMed

    Wang, Kai-Lee; Huang, Wen-Ching; Chou, Jou-Chun; Weng, Ting-Chun; Hu, Sindy; Lieu, Fu-Kong; Lai, Wei-Ho; Idova, Galina; Wang, Paulus S; Wang, Shyi-Wu

    2016-07-01

    A positive correlation between smoking and hypertension has been well established. Acrolein is a major toxic volatile compound found in cigarette smoke. Human exposure to low levels of acrolein is unavoidable due to its production in daily activities, such as smoke from industrial, hot oil cooking vapors, and exhaust fumes from vehicles. The toxicity and the action mechanism of acrolein to induce apoptosis have been extensively studied, but the effects of acrolein on hypertension are still unknown. The present study aimed to examine the effects of acrolein on aldosterone release both in vivo and in vitro. Male rats were divided into three groups, and intraperitoneally injected with normal saline, or acrolein (2mg/kg) for 1 (group A-1) or 3 (group A-3) days, respectively. After sacrificing, rat blood samples were obtained to measure plasma aldosterone and angiotensin II (Ang II) levels. Zona glomerulosa (ZG) cells were prepared from rat adrenal cortex, and were incubated with or without stimulants. We found that the serum aldosterone was increased by 1.2-fold (p<0.05) in A-3 group as compared to control group. Basal aldosterone release from ZG cells in A-3 group was also increased significantly. Moreover, acrolein enhanced the stimulatory effects of Ang II and 8-bromo-cyclic AMP on aldosterone secretion from ZG cells prepared in both A-1 and A-3 groups. Furthermore, the enzyme activity of P450scc, the rate-limiting step of aldosterone synthesis, was elevated after acrolein injection. Plasma level of Ang II was increased in both A-1 and A-3 groups. These results suggested that acrolein exposure increased aldosterone production, at least in part, through elevating the level of plasma Ang II and stimulating steroidogenesis pathways. Copyright © 2016 Elsevier Inc. All rights reserved.

  5. Enhanced contraception of canine zona pellucida 3 DNA vaccine via targeting DEC-205 in mice.

    PubMed

    Wang, Ying; Zhang, Beibei; Li, Jinyao; Aipire, Adila; Li, Yijie; Zhang, Fuchun

    2018-06-01

    Zona pellucida 3 (ZP3) is a potential antigen for the development of contraceptive vaccines to control animal population. In this study, we designed a canine ZP3 (CZP3) DNA vaccine through targeting DEC-205 (named as pcD-scFv-CZP3c) and investigated its contraceptive effect in mice. Female BALB/c mice were intramuscularly immunized 3 times at 2 weeks intervals. After immunization, humoral and cellular immune responses were detected by ELISA and flow cytometry. The results showed that pcD-CZP3 and pcD-scFv-CZP3c induced CZP3-specific antibody (Ab) responses both in serum and vaginal secretions compared to pcDNA3.1. Additionally, compared to pcD-CZP3, pcD-scFv-CZP3c increased the levels of CZP3-specific Abs after a third immunization. Abs induced by these two DNA vaccines could bind with mice and dogs oocytes. Moreover, pcD-scFv-CZP3c enhanced the activation of CD4 + T cells characterized by the increased frequencies of CD4 + CD44 + T cells. Finally, the contraceptive effect was evaluated in the immunized mice. These two DNA vaccines significantly decreased a mean litter size of mice compared to pcDNA3.1, but pcD-scFv-CZP3c group showed the smallest mean litter size. The mean litter size of pcD-scFv-CZP3 were 3.2 ± 0.742 and 4.6 ± 1.118 in two mating tests, which were significantly lower than pcDNA3.1(P < 0.001 and P < 0.05). Our results suggest that the CZP3 DNA vaccine targeted with DEC-205 may be a potential strategy for developing a contraceptive DNA vaccine. Copyright © 2018 Elsevier Inc. All rights reserved.

  6. Left centro-parieto-temporal response to tool-gesture incongruity: an ERP study.

    PubMed

    Chang, Yi-Tzu; Chen, Hsiang-Yu; Huang, Yuan-Chieh; Shih, Wan-Yu; Chan, Hsiao-Lung; Wu, Ping-Yi; Meng, Ling-Fu; Chen, Chen-Chi; Wang, Ching-I

    2018-03-13

    Action semantics have been investigated in relation to context violation but remain less examined in relation to the meaning of gestures. In the present study, we examined tool-gesture incongruity by event-related potentials (ERPs) and hypothesized that the component N400, a neural index which has been widely used in both linguistic and action semantic congruence, is significant for conditions of incongruence. Twenty participants performed a tool-gesture judgment task, in which they were asked to judge whether the tool-gesture pairs were correct or incorrect, for the purpose of conveying functional expression of the tools. Online electroencephalograms and behavioral performances (the accuracy rate and reaction time) were recorded. The ERP analysis showed a left centro-parieto-temporal N300 effect (220-360 ms) for the correct condition. However, the expected N400 (400-550 ms) could not be differentiated between correct/incorrect conditions. After 700 ms, a prominent late negative complex for the correct condition was also found in the left centro-parieto-temporal area. The neurophysiological findings indicated that the left centro-parieto-temporal area is the predominant region contributing to neural processing for tool-gesture incongruity in right-handers. The temporal dynamics of tool-gesture incongruity are: (1) firstly enhanced for recognizable tool-gesture using patterns, (2) and require a secondary reanalysis for further examination of the highly complicated visual structures of gestures and tools. The evidence from the tool-gesture incongruity indicated altered brain activities attributable to the N400 in relation to lexical and action semantics. The online interaction between gesture and tool processing provided minimal context violation or anticipation effect, which may explain the missing N400.

  7. Evaluation of zona pellucida birefringence intensity during in vitro maturation of oocytes from stimulated cycles.

    PubMed

    Petersen, Claudia G; Vagnini, Laura D; Mauri, Ana L; Massaro, Fabiana C; Silva, Liliane F I; Cavagna, Mario; Baruffi, Ricardo L R; Oliveira, Joao B A; Franco, José G

    2011-04-23

    This study evaluated whether there is a relationship between the zona pellucida birefringence (ZP-BF) intensity and the nuclear (NM) and cytoplasmic (CM) in vitro maturation of human oocytes from stimulated cycles. The ZP-BF was evaluated under an inverted microscope with a polarizing optical system and was scored as high/positive (when the ZP image presented a uniform and intense birefringence) or low/negative (when the image presented moderate and heterogeneous birefringence). CM was analyzed by evaluating the distribution of cortical granules (CGs) throughout the ooplasm by immunofluorescence staining. CM was classified as: complete, when CG was localized in the periphery; incomplete, when oocytes presented a cluster of CGs in the center; or in transition, when oocytes had both in clusters throughout cytoplasm and distributed in a layer in the cytoplasm periphery Nuclear maturation: From a total of 83 germinal vesicle (GV) stage oocytes, 58 of oocytes (69.9%) reached NM at the metaphase II stage. From these 58 oocytes matured in vitro, the high/positively scoring ZP-BF was presented in 82.7% of oocytes at the GV stage, in 75.8% of oocytes when at the metaphase I, and in 82.7% when oocytes reached MII. No relationship was observed between NM and ZP-BF positive/negative scores (P = 0.55). These variables had a low Pearson's correlation coefficient (r = 0.081). Cytoplasmic maturation: A total of 85 in vitro-matured MII oocytes were fixed for CM evaluation. Forty-nine oocytes of them (57.6%) showed the complete CM, 30 (61.2%) presented a high/positively scoring ZP-BF and 19 (38.8%) had a low/negatively scoring ZP-BF. From 36 oocytes (42.3%) with incomplete CM, 18 (50%) presented a high/positively scoring ZPBF and 18 (50%) had a low/negatively scoring ZP-BF. No relationship was observed between CM and ZP-BF positive/negative scores (P = 0.42). These variables had a low Pearson's correlation coefficient (r = 0.11). The current study demonstrated an absence of

  8. Pronuclear formation by ICSI using chemically activated ovine oocytes and zona pellucida bound sperm.

    PubMed

    Hernández-Pichardo, J E; Ducolomb, Y; Romo, S; Kjelland, M E; Fierro, R; Casillas, F; Betancourt, M

    2016-01-01

    In order to improve ICSI, appropiate sperm selection and oocyte activation is necessary. The objective of the present study was to determine the efficiency of fertilization using ICSI with chemically activated ovine oocytes and sperm selected by swim up (SU) or swim up + zona pellucida (SU + ZP) binding. Experiment 1, 4-20 replicates with total 821 in vitro matured oocytes were chemically activated with ethanol, calcium ionophore or ionomycin, to determine oocyte activation (precense of one PN). Treatments showed similar results (54, 47, 42 %, respectively) but statistically differents ( P  < 0.05) than mechanical activated oocytes in sham, ICSI and sham injection (13, 25, 32 %, respectively) (10-17 replicates; n  = 429). Experiment 2: Twelve ejaculates and 28 straws of semen were used (11-19 replicates). Sperm were selected by SU in BSA-TCM 199-H medium. A total of 2,294 fresh sperm and 2,760 from frozen-thawed semen were analyzed after SU or SU + ZP binding. Fresh sperm selected by SU showed acrosome reaction (AR) of 59 %, the sperm selected by SU + ZP binding increased AR to 91 %. In comparison, the AR of frozen-thawed sperm using SU or SU + ZP binding was 77 and 86 %, respectively ( P  < 0.05). Experiment 3: fertilization in 200 mechanical activativated oocytes (17 replicates) was 4 %, but fertilization increased in ethanol activated oocytes after ICSI (12-28 %) (5-6 replicates). When fresh sperm only selected by SU were injected to 123 oocytes, a fertilization rate (28 %) was achieved; in sperm selected by SU + ZP was 25 % (73 oocytes). In comparison, in frozen-thawed sperm selected by SU, fertilization was 13 % (70 oocytes), whereas sperm from SU + ZP binding displayed 12 % (51 oocytes) ( P  > 0.05). Chemical activation induces higher ovine oocyte activation than mechanical activation. Ethanol slightly displays higher oocyte activation than calcium ionophore and ionomicine. Sperm selection with SU

  9. Two subunits of the 55 K porcine zona pellucida glycoprotein family are immunologically distinct

    SciTech Connect

    Subramanian, M.G.; Yurewicz, E.C.; Sacco, A.G.

    1986-03-01

    The 55K glycoprotein family (ZP3) of the porcine zona pellucida is comprised of two subunits of 46 K and 45 K which can be resolved by endo-..beta..-galactosidase digestion of ZP3 followed by reversed phase HPLC on Vydac C4 resin. Gel electrophoresis revealed that the 46 K component (EBDG..cap alpha..) is approx. 95% pure and the 45 K component (EBGD..beta..) is 100% pure. In the present study, these two subunits were evaluated immunologically by RIA. Under similar reaction protocols (chloramine-T iodination procedure) comparable specific activities were obtained for EBGD..cap alpha.. (33.06 +/- 7.5 ..mu..ci/..mu..gm), EBGD..beta.. (30.45 +/- 1.6) and ZP3 (26.3more » +/- 1.3). Antibody (Ab) titration studies revealed that EBGD..cap alpha.. and ..beta.. are potent immunogens and /sup 125/I-EBGD..cap alpha.. showed minimal cross reactivity to EBGD..beta..-Ab (8% bound at 1:500 dilution), whereas, /sup 125/I-EBGD..beta.. showed a greater degree of cross reactivity to EBGD..cap alpha..-Ab (23% bound at 1:500 dilution). Maximum binding for the two labeled antigens against homologous Abs (1:500) was > 60%. Dose response studies revealed that in the /sup 125/I-EBGD..cap alpha.. vs EBGD..cap alpha.. -Ab system, the 50% intercept was 3.25 +/- 0.32 ng for EBGD..cap alpha.. and 472.43 +/- 30.26 ng for EBGD..beta.. (p < 0.01), whereas, in the /sup 125/I-EBGD..beta.. vs EBGD..beta..-Ab system the 50% intercept was 3.51 +/- 0.58 for EBGD..beta.. and 166.77 +/- 49.20 for EBGD..cap alpha.. (p < 0.01). No significant differences were observed in the slopes of the dose response curves. It is concluded that the two subunits of ZP3 possess distinct immunologic characteristics as evaluated by RIA.« less

  10. Surface alterations of the mouse zona pellucida and ovum following in vivo fertilization: correlation with the cell cycle

    SciTech Connect

    Jackowski, S.; Dumont, J.N.

    1979-01-01

    The zona pellucida and cell surface of in vivo fertilized mouse ova exhibit time dependent changes which can be detected with the scanning electron microscope. The periods of ovulation, fertilization and first cleavage in superovulated C3D2/F/sub 1/ hybrids were determined and times corresponding to G/sub 1/, S, G/sub 2/, and M were calculated. The zona of a mature unfertilized ovum has a rough texture with deep furrows; at fertilization and thereafter the zona develops a smoother, ropy and seemingly porous surface. The cell surface of the unfertilized ovum is characterized by uniform microvilli, small blebs and rounded, mound-like elevations. Aftermore » fertilization and development to G/sub 1/, the ovum loses its blebs but retains the mound-like elevations and microvilli which are now less uniform. As the ovum progresses toward S, it loses the mound-like elevations but retains microvilli in the same density as found in G/sub 1/. The ovum in G/sub 2/ exhibits smaller but more numerous microvilli which vary considerably in length. Some appear to bifurcate. The fertilized ovum developing through M and G/sub 1/ of the 2 cell stage exhibits a less dense population of relatively uniform microvilli, periodic blebs and, again, rounded elevations. The data are reminiscent of surface changes associated with the cell cycle in tissue culture cells and indicate a cyclic progression of the in vivo fertilized mouse ovum through the first cleavage division to the 2 cell stage.« less

  11. Modified Team-Based Learning Strategy to Improve Human Anatomy Learning: A Pilot Study at the Universidad Del Norte in Barranquilla, Colombia

    ERIC Educational Resources Information Center

    Martínez, Emilio G.; Tuesca, Rafael

    2014-01-01

    As part of an institutional program sponsored by the Centre for Teaching Excellence at the Universidad del Norte, Barranquilla, Colombia, we developed an educational research study on two sessions of human anatomy in which we combined team-based learning (TBL) and the use of iPads. Study data included the TBL, assessments applied during the…

  12. Assessment of Mouse Germinal Vesicle Stage Oocyte Quality by Evaluating the Cumulus Layer, Zona Pellucida, and Perivitelline Space

    PubMed Central

    Liu, Ying-Lei; Chen, Ying; Zhou, Cheng-Jie; Wu, Sha-Na; Shen, Jiang-Peng; Liang, Cheng-Guang

    2014-01-01

    To improve the outcome of assisted reproductive technology (ART) for patients with ovulation problems, it is necessary to retrieve and select germinal vesicle (GV) stage oocytes with high developmental potential. Oocytes with high developmental potential are characterized by their ability to undergo proper maturation, fertilization, and embryo development. In this study, we analyzed morphological traits of GV stage mouse oocytes, including cumulus cell layer thickness, zona pellucida thickness, and perivitelline space width. Then, we assessed the corresponding developmental potential of each of these oocytes and found that it varies across the range measured for each morphological trait. Furthermore, by manipulating these morphological traits in vitro, we were able to determine the influence of morphological variation on oocyte developmental potential. Manually altering the thickness of the cumulus layer showed strong effects on the fertilization and embryo development potentials of oocytes, whereas manipulation of zona pellucida thickness effected the oocyte maturation potential. Our results provide a systematic detailed method for selecting GV stage oocytes based on a morphological assessment approach that would benefit for several downstream ART applications. PMID:25144310

  13. GABA release in the zona incerta of the sheep in response to the sight and ingestion of food and salt.

    PubMed

    Kendrick, K M; Hinton, M R; Baldwin, B A

    1991-05-31

    In order to establish which neurotransmitters may influence the activity of zona incerta neurones in the sheep which respond selectively to the sight or ingestion of food, we have measured the release of amino acid and monoamine neurotransmitters from this region using microdialysis sampling. Co-ordinates for the placement of microdialysis probes in regions of the zona incerta where cells respond to the sight or ingestion of food were first established by making single-unit extracellular recordings. When animals were food-deprived results showed that release of gamma-aminobutyric acid (GABA) was increased in response to the sight and ingestion of food but not of aspartate, glutamate, taurine, noradrenaline, dopamine or serotonin. This release of GABA was absent when the animals were shown non-food objects or saw or ingested salt solutions. When the same animals were physiologically sodium-depleted GABA release was evoked by the sight and ingestion of salt solutions and release following the sight and ingestion of food was significantly reduced. These results provide further evidence that GABA is an important neurotransmitter in neural circuits controlling the regulation of food intake.

  14. Incentivos para atraer y retener personal de salud de zonas rurales del Perú: un estudio cualitativo

    PubMed Central

    Huicho, Luis; Canseco, Francisco Díez; Lema, Claudia; Miranda, J. Jaime; Lescano, Andrés G.

    2014-01-01

    El objetivo fue identificar incentivos de atracción y retención en zonas rurales y distantes de Ayacucho, Perú. Fueron realizadas entrevistas en profundidad con 80 médicos, enfermeras, obstetras y técnicos (20 por grupo) de las zonas más pobres y con 11 funcionarios. No existen políticas sistemáticas de atracción y retención de personal de salud en Ayacucho. Los principales incentivos, en orden de importancia, fueron mejoras salariales, oportunidades de formación y capacitación, estabilidad laboral y nombramiento, mejoras en infraestructura y equipos, e incremento del personal. Se mencionaron también mejoras en la vivienda y alimentación, mayor cercanía con la familia y reconocimiento por el sistema de salud. Existen coincidencias y singularidades entre los distintos grupos sobre los incentivos clave para estimular el trabajo rural, que deben considerarse al diseñar políticas públicas. Las iniciativas del Estado deben comprender procesos rigurosos de monitoreo y evaluación, para asegurar que las mismas tengan el impacto deseado. PMID:22488318

  15. Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona binding.

    PubMed

    Hou, S T; Ma, A; Jones, R; Hall, L

    1996-10-01

    The rat sperm surface antigen, 2B1, that has been proposed to play a key role in sperm adhesion to the zona pellucida, has been cloned and its entire cDNA sequenced. Northern blot analysis indicates that 2B1 is encoded by a 2.2-kb RNA transcript that is abundantly expressed in the testis. The deduced protein sequence contains 512 amino-acid residues with a strong candidate signal sequence and C-terminal transmembrane domain. Data base searches reveal a high degree of sequence similarity to guinea pig, rabbit, monkey, and human PH20 sperm surface antigens, and a lower degree of similarity to honey bee and whiteface hornet venom hyaluronidases. Rat 2B1 antigen also possesses hyaluronidase activity, suggesting that it is a bifunctional protein with putative roles in the dispersion of cumulus oophorus cells as well as zona adhesion. However, while it would appear that 2B1 is the rat homologue of the guinea pig PH20 antigen, they differ in a number of important biochemical respects (including their mode of attachment to the sperm membrane and distribution between soluble and membrane-bound fractions), as well as in their localization on the sperm membrane. Expression of regions of the 2B1 protein in recombinant bacterial cells has allowed a preliminary mapping of the 2B1 epitope, and has provided more definitive information on the endoproteolytic processing of 2B1 during epididymal transit.

  16. Animal-based folk remedies sold in public markets in Crato and Juazeiro do Norte, Ceará, Brazil.

    PubMed

    Ferreira, Felipe S; Brito, Samuel V; Ribeiro, Samuel C; Saraiva, Antônio A F; Almeida, Waltécio O; Alves, Rômulo R N

    2009-06-03

    Human communities consistently develop a detailed knowledge of the therapeutical and medicinal properties of the local flora and fauna, and these folk remedies often substitute medicines produced by the pharmaceutical industry. Animals (and their derived products) are essential ingredients in the preparation of many traditional remedies. The present work prepared an inventory of the animals sold in public markets in the cities of Crato and Juazeiro do Norte, Ceará State, Brazil. Information was obtained through the use of semi-structured questionnaires in interviews held with 27 merchants of medicinal animals (18 in the municipality of Juazeiro do Norte [11 men and 7 women] and 9 people in the municipality of Crato [6 men and 3 women]). We calculated the Informant Consensus Factor (ICF) to determine the consensus over which species are effective for particular ailments, as well as the species Use Value (UV) to determine the extent of utilization of each species. A total of 31 animal species, distributed among 21 families were identified as being used medicinally. The taxa most represented were: insects (8 species), mammals (7), fish (5), reptiles (5) and birds (4). The animals sold in these markets are used to treat a total of 24 ailments, with rheumatism, asthma, and inflammations having the largest numbers of citations. Three species not previously reported as having medicinal use were encountered: Leporinus steindachneri (utilized for treating cholesterol problems), Gryllus assimilis (utilized in treating urinary infections), and Phrynops tuberosus (used to treat asthma, rheumatism and bruises). The composition of the local fauna, the popular culture, and commercial considerations are factors that maintain and drive the market for therapeutic animal products - and the lack of monitoring and regulation of this commerce is worrisome from a conservationist perspective. A detailed knowledge of the fauna utilized in alternative medicine is fundamental to the

  17. Animal-based folk remedies sold in public markets in Crato and Juazeiro do Norte, Ceará, Brazil

    PubMed Central

    Ferreira, Felipe S; Brito, amuel V; Ribeiro, Samuel C; Saraiva, Antônio AF; Almeida, Waltécio O; Alves, Rômulo RN

    2009-01-01

    Background Human communities consistently develop a detailed knowledge of the therapeutical and medicinal properties of the local flora and fauna, and these folk remedies often substitute medicines produced by the pharmaceutical industry. Animals (and their derived products) are essential ingredients in the preparation of many traditional remedies. The present work prepared an inventory of the animals sold in public markets in the cities of Crato and Juazeiro do Norte, Ceará State, Brazil. Methods Information was obtained through the use of semi-structured questionnaires in interviews held with 27 merchants of medicinal animals (18 in the municipality of Juazeiro do Norte [11 men and 7 women] and 9 people in the municipality of Crato [6 men and 3 women]). We calculated the Informant Consensus Factor (ICF) to determine the consensus over which species are effective for particular ailments, as well as the species Use Value (UV) to determine the extent of utilization of each species. Results A total of 31 animal species, distributed among 21 families were identified as being used medicinally. The taxa most represented were: insects (8 species), mammals (7), fish (5), reptiles (5) and birds (4). The animals sold in these markets are used to treat a total of 24 ailments, with rheumatism, asthma, and inflammations having the largest numbers of citations. Three species not previously reported as having medicinal use were encountered: Leporinus steindachneri (utilized for treating cholesterol problems), Gryllus assimilis (utilized in treating urinary infections), and Phrynops tuberosus (used to treat asthma, rheumatism and bruises). Conclusion The composition of the local fauna, the popular culture, and commercial considerations are factors that maintain and drive the market for therapeutic animal products – and the lack of monitoring and regulation of this commerce is worrisome from a conservationist perspective. A detailed knowledge of the fauna utilized in

  18. An Evaluation of the El Centro de la Causa Library and Information Center: August 1973 through July 1974. Final Report.

    ERIC Educational Resources Information Center

    Michael, Mary Ellen; Encarnacion, Leticia

    An evaluation of Chicago's El Centro de la Causa Library and Information Center was undertaken by the University of Illinois Library Research Center in 1974. Evaluation methods included: (1) a survey of user and nonuser characteristics and attitudes concerning library services; (2) a survey of the needs and information-seeking behavior of people…

  19. Resveratrol reverses the adverse effects of a diet-induced obese murine model on oocyte quality and zona pellucida softening.

    PubMed

    Jia, Zhenzhen; Feng, Zeyang; Wang, Lining; Li, Hao; Wang, Hongyu; Xu, Dingqi; Zhao, Xin; Feng, Daofu; Feng, Xizeng

    2018-05-23

    Reproductive dysfunction associated with obesity is increasing among women of reproductive age, including infertility and increasing risk of miscarriage. In females, reproductive disorders are linked to declining quality of oocytes. Using a model of diet-induced obesity, we have investigated the possible effects of obesity on oocyte quality, including metabolism, lipid accumulation, ROS levels, meiosis and changes in spindle structure in Metaphase II. Our study showed that obesity induced by a high fat diet can impair oocyte meiosis, destroy spindle assembly, and promote oxidative stress and abnormal mitochondrial distribution. With the addition of resveratrol, the negative impact of diet-induced obesity on the quality of oocytes was alleviated to some extent. In addition, we found that obesity causes mouse oocytes to soften, and resveratrol can restore the zona pellucida of oocytes to the same state as the control group. In conclusion, resveratrol can reverse the adverse effects of obesity on oocytes, which is beneficial for subsequent embryonic development.

  20. A common 'aggregation-prone' interface possibly participates in the self-assembly of human zona pellucida proteins.

    PubMed

    Louros, Nikolaos N; Chrysina, Evangelia D; Baltatzis, Georgios E; Patsouris, Efstratios S; Hamodrakas, Stavros J; Iconomidou, Vassiliki A

    2016-03-01

    Human zona pellucida (ZP) is composed of four glycoproteins, namely ZP1, ZP2, ZP3 and ZP4. ZP proteins form heterodimers, which are incorporated into filaments through a common bipartite polymerizing component, designated as the ZP domain. The latter is composed of two individually folded subdomains, named ZP-N and ZP-C. Here, we have synthesized six 'aggregation-prone' peptides, corresponding to a common interface of human ZP2, ZP3 and ZP4. Experimental results utilizing electron microscopy, X-ray diffraction, ATR FT-IR spectroscopy and polarizing microscopy indicate that these peptides self-assemble forming fibrils with distinct amyloid-like features. Finally, by performing detailed modeling and docking, we attempt to shed some light in the self-assembly mechanism of human ZP proteins. © 2016 Federation of European Biochemical Societies.

  1. Ground-water resources of the Acu Valley, Rio Grande Norte, Brazil

    USGS Publications Warehouse

    Rodis, Harry G.; de Castro Araujo, Jonas Maria.

    1968-01-01

    The Acu Valley is the lower part of the Rio Piranhas valley in the northwestern part of the State of Rio Grande do Norte, Brazil. It begins where the Rio Piranhas leaves the crystalline Precambrian rocks to flow across the outcrop of sedimentary rocks. The area considered in this report extends northward for about 45 kilometers; it is terminated arbitrarily where encroachment by sea water has contaminated the aquifer and imparted a disagreeable saline taste to the water in it. The boundary was not determined in the field, however, for lack of special equipment. Part of the extensive uplands on either side of the valley are included. This makes the total area approximately 2,500 square kilometers. The largest town, Acu, had a population of about 8,000 in 1960. The area is considered to be part of the Drought Polygon of northeast Brazil because the precipitation, although averaging 448 millimeters annually at Acu, varies widely from year to year and often is deficient for many months. The precipitation has been supplemented by use of irrigation wells, but irrigated agriculture is not yet far advanced, and the quantities of water used in irrigation are small. Geologically, the area consists of basement crystalline rocks (Precambrian), a wedge of sedimentary rocks thickening northward (Cretaceous), and alluvial sediments constituting a narrow band in the bottom of the valley (Alluvium and terrace deposits). The crystalline rocks contain water mainly in fractures and, in general, are impermeable. The sedimentary rocks of Cretaceous age comprise two units: a thick but fine-grained sandstone grading upward into siltstone and shale (Acu Sandstone), and limestone and dolomite with an included shale zone (Jandaira Limestone). The sandstone especially and the limestone to a lesser degree are ground-water reservoirs of large capacity. The limestone has been tapped at several places, but the sandstone and its contained water are practically untested and, hence, imperfectly

  2. Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm

    PubMed Central

    Patrat, Catherine; Auer, Jana; Fauque, Patricia; Leandri, Roger L; Jouannet, Pierre; Serres, Catherine

    2006-01-01

    Background The functions of three zona glycoproteins, ZP1, ZP2 and ZP3 during the sperm-zona pellucida (ZP) interaction are now well established in mice. The expression of an additional zona glycoprotein, ZPB/4, in humans, led us to reconsider the classical mouse model of gamete interaction. We investigated the various functions of human ZP (hZP) during the interaction of spermatozoa with fertilised and unfertilised oocytes. Results The hZP of fertilised oocytes retained their ability to bind sperm (albeit less strongly than that from unfertilised oocytes), to induce an intraspermatic calcium influx through voltage-dependent channels similar to that observed with hZP from unfertilised oocytes and to promote the acrosome reaction at a rate similar to that induced by the ZP of unfertilised oocytes (61.6 ± 6.2% vs60.7 ± 9.1% respectively). Conversely, the rate of hZP penetrated by sperm was much lower for fertilised than for unfertilised oocytes (19% vs 57% respectively, p < 0.01). We investigated the status of ZP2 in the oocytes used in the functional tests, and demonstrated that sperm binding and acrosome reaction induction, but not ZP penetration, occurred whether or not ZP2 was cleaved. Conclusion The change in ZP function induced by fertilisation could be different in human and mouse species. Our results suggest a zona blocking to polyspermy based at the sperm penetration level in humans. PMID:17147816

  3. Regulation of acrosomal exocytosis. II. The zona pellucida-induced acrosome reaction of bovine spermatozoa is controlled by extrinsic positive regulatory elements.

    PubMed

    Florman, H M; First, N L

    1988-08-01

    The effects of accessory sex gland secretions on the zona pellucida-induced acrosome reaction of bovine spermatozoa were investigated. Soluble extracts of zonae pellucidae initiated exocytosis in ejaculated spermatozoa. This process had an ED50 of 20 ng/microliter zona pellucida protein and saturated at 50 ng/microliter (Florman and First, 1988. Dev. Biol. 128, 453-463). In epididymal sperm this dose-response relationship was shifted toward greater agonist concentrations by at least a factor of 10(3). Reconstitution of high potency agonist response was achieved in vitro by incubation of epididymal sperm with bovine seminal plasma. Reconstitution was dependent on the seminal plasma protein concentration. The ED50 of this process was 62 micrograms protein/10(8) sperm and saturation was observed with 124 micrograms protein/10(8) sperm. Agonist responses in reconstituted epididymal sperm and in ejaculated sperm were indistinguishable with regard to dependence on the zona pellucida protein concentration and the kinetics of induced acrosome reactions. Kinetic studies suggest that reconstitution is due to adsorption of regulatory factors from seminal plasma. In addition to the positive regulatory elements responsible for reconstituting activity, seminal plasma also contains negative regulatory elements which inhibit agonist response. These negative factors are inactivated during sperm capacitation, permitting the expression of positive regulators. Acting together, these regulatory elements could coordinate high affinity agonist response with the availability of eggs in vivo.

  4. Articulatory Closure Proficiency in Patients with Parkinson's Disease Following Deep Brain Stimulation of the Subthalamic Nucleus and Caudal Zona Incerta

    ERIC Educational Resources Information Center

    Karlsson, Fredrik; Olofsson, Katarina; Blomstedt, Patric; Linder, Jan; Nordh, Erik; van Doorn, Jan

    2014-01-01

    Purpose: The present study aimed at comparing the effects of deep brain stimulation (DBS) treatment of the subthalamic nucleus (STN) and the caudal zona incerta (cZi) on the proficiency in achieving oral closure and release during plosive production of people with Parkinson's disease. Method: Nineteen patients participated preoperatively and…

  5. [Effects of infrasound on activities of 3beta hydroxysteroid dehydrogenase and acid phosphatase of polygonal cells in adrenal cortex zona fasciculate in mice].

    PubMed

    Dang, Wei-min; Wang, Sheng; Tian, Shi-xiu; Chen, Bing; Sun, Fei; Li, Wei; Jiao, Yan; He, Li-hua

    2007-02-01

    To explore the biological effects of infrasound on the polygonal cells in adrenal cortex zona fasciculation in mice. The biological effects of infrasound on the activities of 3beta hydroxysteroid dehydrogenase (3-betaHSDH) and acid phosphatase(ACP) of the polygonal cells in adrenal cortex zona fasciculate were observed when exposure to 8 and 16 Hz infrasound at 80, 90, 100, 110, 120 and 130 dB for 1 day, 7 days and 14 days or 14 days after the exposure. When exposure to 8 Hz infrasound, the enzyme activities of 3-betaHSDH increase as the sound pressure levels increase. Only when the sound pressure levels reach 130 dB, the enzyme activities began to decrease exceptionally. When exposure to 16 Hz, 80 dB infrasound, no significant difference between the treatment and control group in the activities of 3-betaHSDH could be observed, but the injury of the polygonal cells had appeared. When exposure to 16 Hz, 100 dB infrasound, the activities of 3-betaHSDH started to increase. The cell injury still existed. When exposed to 16 Hz, 120 dB infrasound, the local tissue damage represented. Fourteen days after the mice exposure to 8 Hz, 90 dB and 130 dB infrasound for 14 days continuously, the local tissue injury of the adrenal cortex zona fasciculation began to recover at certain extent, but the higher the exposure sound pressure level, the poorer the tissue recovery. The biological effects of infrasound on the polygonal cells in adrenal cortex zona fasciculation response to the frequency of the infrasound are found at certain action strength range, but this characteristic usually is covered by the severe tissue injury. When exposure to infrasound is stopped for a period of time, the local tissue injury of the adrenal cortex zona fasciculation could recovers at certain extent, but the higher the exposure sound pressure level, the more poorer the tissue recovery.

  6. Determination of βS haplotypes in patients with sickle-cell anemia in the state of Rio Grande do Norte, Brazil

    PubMed Central

    Cabral, Cynthia Hatsue Kitayama; Serafim, Édvis Santos Soares; de Medeiros, Waleska Rayane Dantas Bezerra; de Medeiros Fernandes, Thales Allyrio Araújo; Kimura, Elza Miyuki; Costa, Fernando Ferreira; de Fátima Sonati, Maria; Rebecchi, Ivanise Marina Moretti; de Medeiros, Tereza Maria Dantas

    2011-01-01

    βS haplotypes were studied in 47 non-related patients with sickle-cell anemia from the state of Rio Grande do Norte, Brazil. Molecular analysis was conducted by PCR/RFLP using restriction endonucleases XmnI, HindIII, HincII and HinfI to analyze six polymorphic sites from the beta cluster. Twenty-seven patients (57.5%) were identified with genotype CAR/CAR, 9 (19.1%) CAR/BEN, 6 (12.8%) CAR/CAM, 1 (2.1%) BEN/BEN, 2 (4.3%) CAR/Atp, 1 (2.1%) BEN/Atp and 1 (2.1%) with genotype Atp/Atp. The greater frequency of Cameroon haplotypes compared to other Brazilian states suggests the existence of a peculiarity of African origin in the state of Rio Grande do Norte. PMID:21931513

  7. Involvement of adenosine monophosphate activated kinase in interleukin-6 regulation of steroidogenic acute regulatory protein and cholesterol side chain cleavage enzyme in the bovine zona fasciculata and zona reticularis.

    PubMed

    De Silva, Matharage S I; Dayton, Adam W; Rhoten, Lance R; Mallett, John W; Reese, Jared C; Squires, Mathieu D; Dalley, Andrew P; Porter, James P; Judd, Allan M

    2018-06-01

    In bovine adrenal zona fasciculata (ZF) and NCI-H295R cells, interleukin-6 (IL-6) increases cortisol release, increases expression of steroidogenic acute regulatory protein (StAR), cholesterol side chain cleavage enzyme (P450scc), and steroidogenic factor 1 (SF-1) (increases steroidogenic proteins), and decreases the expression of adrenal hypoplasia congenita-like protein (DAX-1) (inhibits steroidogenic proteins). In contrast, IL-6 decreases bovine adrenal zona reticularis (ZR) androgen release, StAR, P450scc, and SF-1 expression, and increases DAX-1 expression. Adenosine monophosphate (AMP) activated kinase (AMPK) regulates steroidogenesis, but its role in IL-6 regulation of adrenal steroidogenesis is unknown. In the present study, an AMPK activator (AICAR) increased (P < 0.01) NCI-H295R StAR promoter activity, StAR and P450scc expression, and the phosphorylation of AMPK (PAMPK) and acetyl-CoA carboxylase (PACC) (indexes of AMPK activity). In ZR (decreased StAR, P450scc, SF-1, increased DAX-1) (P < 0.01) and ZF tissues (increased StAR, P450scc, SF-1, decreased DAX-1) (P < 0.01), AICAR modified StAR, P450scc, SF-1 and DAX-1 mRNAs/proteins similar to the effects of IL-6. The activity (increased PAMPK and PACC) (P < 0.01) of AMPK in the ZF and ZR was increased by AICAR and IL-6. In support of an AMPK role in IL-6 ZF and ZR effects, the AMPK inhibitor compound C blocked (P < 0.01) the effects of IL-6 on the expression of StAR, P450scc, SF-1, and DAX-1. Therefore, IL-6 modification of the expression of StAR and P450scc in the ZF and ZR may involve activation of AMPK and these changes may be related to changes in the expression of SF-1 and DAX-1. Copyright © 2018 Elsevier Inc. All rights reserved.

  8. [Clinical study of fire acupuncture with centro-square needles for knee osteoarthritis].

    PubMed

    Wang, Bing; Hu, Jing; Zhang, Ning; Wang, Jingjing; Chen, Zhongjie; Wu, Zhongchao

    2017-05-12

    To compare the efficacy difference between fire acupuncture with centro-square needles (FACSN) and filiform needling (FN) for knee osteoarthritis (KOA). Seventy-two patients were randomly assigned into an FACSN group and an FN group, 36 cases in each one. Ashi points, Xuehai (SP 10), Liangqiu (ST 34), Neixiyan (EX-LE 4), Dubi (ST 35), Zusanli (ST 36), Yanglingquan (GB 34) and Yinlingquan (SP 9) were selected in the two groups. The FACSN group was treated with FACSN, and three acupoints were selected for each treatment; the FN group was treated with FN, and all the acupoints were selected for each treatment. The cupping treatment was given after acupuncture in the two groups. The treatment was given once every other day, without treatment on Sundays. The treatment was given three times a week, 6 times as one course; totally 2 courses were provided. The visual analogue scale (VAS) and Western Ontario and McMaster Universities Arthritis Index (WOMAC) were observed in the two groups before treatment, two weeks, four weeks into treatment and at one-month follow-up visit. In addition, the comprehensive efficacy was compared between the two groups. Compared before treatment, the score of VAS and the total score of WOMAC were improved in the two groups at each time point after treatment (all P <0.01); the scores of VAS at each time point after treatment in FACSN group were lower than those in the FN group (all P <0.05); four weeks into treatment and at one-month follow-up visit, the total score of WOMAC in the FACSN group was lower than that in the FN group (both P <0.05). Two weeks into treatment, the total effective rate was 88.9% (32/36) in the FACSN group, which was higher than 61.1% (22/36) in the FN group ( P <0.01); four weeks into treatment and at one-month follow-up visit, the cured and remarkable effective rates were 66.7% (24/36) and 83.3% (30/36) in the FACSN group, which were higher than 41.7% (15/36) and 44.4% (16/36) in the FN group ( P <0.05, P <0

  9. Direct embryo tagging and identification system by attachment of biofunctionalized polysilicon barcodes to the zona pellucida of mouse embryos.

    PubMed

    Novo, Sergi; Penon, Oriol; Barrios, Leonardo; Nogués, Carme; Santaló, Josep; Durán, Sara; Gómez-Matínez, Rodrigo; Samitier, Josep; Plaza, José Antonio; Pérez-García, Luisa; Ibáñez, Elena

    2013-06-01

    Is the attachment of biofunctionalized polysilicon barcodes to the outer surface of the zona pellucida an effective approach for the direct tagging and identification of cultured embryos? The results achieved provide a proof of concept for a direct embryo tagging system using biofunctionalized polysilicon barcodes, which could help to minimize the risk of mismatching errors (mix-ups) in human assisted reproduction technologies. Even though the occurrence of mix-ups is rare, several cases have been reported in fertility clinics around the world. Measures to prevent the risk of mix-ups in human assisted reproduction technologies are therefore required. Mouse embryos were tagged with 10 barcodes and the effectiveness of the tagging system was tested during fresh in vitro culture (n=140) and after embryo cryopreservation (n = 84). Finally, the full-term development of tagged embryos was evaluated (n =105). Mouse pronuclear embryos were individually rolled over wheat germ agglutinin-biofunctionalized polysilicon barcodes to distribute them uniformly around the ZONA PELLUCIDA surface. Embryo viability and retention of barcodes were determined during 96 h of culture. The identification of tagged embryos was performed every 24 h in an inverted microscope and without embryo manipulation to simulate an automatic reading procedure. Full-term development of the tagged embryos was assessed after their transfer to pseudo-pregnant females. To test the validity of the embryo tagging system after a cryopreservation process, tagged embryos were frozen at the 2-cell stage using a slow freezing protocol, and followed in culture for 72 h after thawing. Neither the in vitro or in vivo development of tagged embryos was adversely affected. The tagging system also proved effective during an embryo cryopreservation process. Global identification rates higher than 96 and 92% in fresh and frozen-thawed tagged embryos, respectively, were obtained when simulating an automatic barcode reading

  10. Deaths on board ships assisted by the Centro Internazionale Radio Medico in the last 25 years.

    PubMed

    Grappasonni, Iolanda; Petrelli, Fabio; Amenta, Francesco

    2012-07-01

    Data on occupational diseases of seafarers and of causes of death during their career are sparse. The causes of deaths on board ships assisted by Centro Internazionale Radio Medico (CIRM), the Italian Telemedical Maritime Assistance Service (TMAS) were reviewed by examining 29,146 files of patients treated from 1986 to 2010. In the 25 years, 383 deaths occurred (1.31%). Diseases of the circulation were the most frequent, followed by external causes such as accidents and violence, infectious and parasitic diseases, alcohol and drug addiction, respiratory system diseases. Cardiovascular and external causes were the principal causes of deaths among seafarers. This investigation is the first study on the causes of death on board ships obtained from data of a maritime telemedical centre, that has assisted seafarers when they were alive or immediately after their death. The fact that diseases of the circulatory system are the first cause of death of sailing seafarers deserves specific initiatives. They should include campaigns for adequate lifestyles and the availability on ships of medical devices useful for diagnostic purposes, resuscitation as well as for verification of death. Copyright © 2012 Elsevier Ltd. All rights reserved.

  11. The transplantation donation process in the Centro de Investigaciones Medico Quirurgicas of Cuba: 1999-2002.

    PubMed

    Abdo, A; Ugarte, J C; Castellanos, R; González, L; López, O; Hernández, J C; Valdivia, J; Almora, E; Suárez, O; Diaz, J; Collera, S; Enamorado, A; Vázquez, A; Beníte, P; Dominguez, J; Wilford, M; Falcon, J

    2003-08-01

    In 1998 in the Centro de Investigaciones Medico Quirurgicas the Transplant Coordination Office (TCO) was created, with the aim to organize a system to support a hepatic transplantation program. This organization, which changed the transplantation-donation process not only in our center but in the whole country, is described in this article. The files of donors generated in our hospital were studied together with the transplant coordination records, from 1999 till the first half of 2002. In the period studied, 21 potential donors were diagnosed with brain death, yielding a donation rate of 71.4%. Brain death was most frequently caused by vascular brain disease; however, in the realized donor group, the cranioencephalic trauma predominated. The typical donor was a man of average age 39.2 years (range, 18-86 years). Among the potential donors, 24% were excluded based on medical criteria, and 5% due to family objections. Forty liver transplantation were performed in 36 patients including 1 liver-kidney simultaneous procedure. The principal etiologies for transplant included hepatitis C virus cirrhosis, 22%; alcoholic, 19%; and acute hepatic failure, 13%. Kidney transplantations were performed in 70 patients, including 41 from cadaveric donors (53.6%) and 29 from living related donors (41.4%). In 2001, a pancreas-kidney transplantation program was started. The creation of the TCO has been of paramount importance to optimize transplantation program functions.

  12. Design of a Heliostat for Centro de Investigaciones de Astronomía (CIDA)

    NASA Astrophysics Data System (ADS)

    Dávila, L. E.; Barboza, S. J.; Sánchez, G. A.; Della Prugna, F.; Cova, J.; Provenzano, S. E.; Chacón, R. D.

    2009-05-01

    This paper explains the procedure used in the design of a heliostat to be installed at the headquarters of the Centro de Investigaciones de Astronomía, located in the city of Mérida, Venezuela. The heliostat will be used mainly for educational and public outreach programs, so the design specifications did not require compensation for rotation of the Sun's image at the focal point. The engineering computational tools CAD-CAE were used in order to allow a seamless interaction between the two disciplines involved, Mechanical Engineering and Astronomy. It has also been taken into consideration, as a starting requirement, that all materials be easily available and that all parts, excluding the optics, could be manufactured with the equipment available at the CIDA workshop. These considerations were intended to reduce the cost of the device and to increase the feasibility of construction with limited technological facilities and financial resources. The results obtained by means of the computing tools used were validated through comparison against the analytical calculations. As a result, a robust but low cost heliostat was designed which in the near future will be used to project the Sun's image on a screen for public viewing and student research projects.

  13. Accidents on board merchant ships. Suggestions based on Centro Internazionale Radio Medico (CIRM) experience.

    PubMed

    Napoleone, Paolo

    2016-01-01

    This statistical study was performed to find out the occurrence of accidents on board ships assisted by Centro Internazionale Radio Medico (CIRM) during the years 2010-2015, with the aim of providing suggestions in accident prevention, based on such a wide experience. The case histories of CIRM in the years 2010-2015 were examined. The total number of accidents per year was calculated and compared as a percentage with the total number of cases assisted by CIRM per year. The incidence of accidents on board in these years ranged between 14.4% and 18.4% of total cases assisted per year, which is constantly increasing. The most common injuries on board among cases treated by CIRM were contusions and wounds. Also burns and eye injuries were significantly represented. Multiple injuries and head injuries were found to be the most frequent cause of death on board due to an accident. More information on the occurrence and type of accidents and on the body injured areas should represent the basis for developing strategies and campaigns for their prevention.

  14. Surf Zone Sediment Size Variation, Morphodynamics, and Hydrodynamics During Sea/Land Breeze and El-Norte Storm in Sisal, Yucatan, Mexico

    NASA Astrophysics Data System (ADS)

    Alrushaid, T.; Figlus, J.; Torres-Freyermuth, A.; Puleo, J. A.; Dellapenna, T. M.

    2016-02-01

    Coastlines around the world are under ever-increasing pressure due to population trends, commerce, and geophysical processes like tropical storms and erosion. This multi-institutional field campaign was conducted to improve our understanding of complex nearshore processes under varying forcing conditions on a microtidal, sandy beach located in Sisal, Yucatan from 3/27 to 4/12/2014. Hydrodynamics, morphodynamics, and textural variability were investigated during: (1) a cold front event (referred to as El-Norte); (2) land breeze (LB); and (3) sea breeze (SB). The instrumentation layout included three surf/swash zone cross-shore transects where water elevation, suspended sediment concentration, bed load, and current velocities were measured, as well as several offshore ADCP for hydrodynamic measurements. TKE, τb, ɛ and were estimated using the data obtained from surf zone ADV. In addition, Hs and Tsin the surf zone were computed using measurements from ADV pressure sensors, while a separate pressure transducer was used to obtain water free-surface elevation within the swash zone. During SB cycles the study area experienced wind velocities reaching up to 12ms-1, and 15ms-1 during El-Norte. Elevated wind stress during El-Norte resulted in Hs of 1.5m and 0.6m in water depths of 10m and 0.4m, respectively. Surface sediment grab samples during SB/LB cycles showed that the swash zone had a moderately well sorted distribution with a mean grain size of 0.5mm, while poor sorting and a mean grain size of 0.7mm were found during El-Norte. Additionally, measured bathymetry data showed evidence for offshore sandbar migration during strong offshore currents (0.4ms-1) during El-Norte, while onshore sandbar migration was evident during SB/LB periods (0.3ms-1 and 0.1ms-1, respectively). This study highlights how different weather forcing conditions affect hydrodynamics, morphodynamics, and textural variability on a sandy beach. Aside from furthering our knowledge on these complex

  15. School of Medicine of Federal University of Rio Grande Do Norte: A traditional curriculum with innovative trends in medical education.

    PubMed

    De Oliveira, Daniel Fernandes Mello; Simas, Breno C C; Guimarães Caldeira, Adrian Lucca; Medeiros, Augusto De Galvão E Brito; Freitas, Marise Reis; Diniz, José; Diniz, Rosiane

    2018-02-28

    The Medical School of the Federal University of Rio Grande do Norte (UFRN) is one of the biggest public medical schools in Northeast Brazil. In the last decade, significant investment in faculty development, innovative learning methodologies and student engagement has been key milestones in educational improvement at this medical school, harnessed to recent political changes that strengthened community-based and emergency education. This study describes how curriculum changes in UFRN Medical School have been responsible for major improvements in medical education locally and which impacts such transformations may have on the educational community. A group of students and teachers revised the new curriculum and established the key changes over the past years that have been responsible for the local enhancement of medical education. This information was compared and contrasted to further educational evidences in order to define patterns that can be reproduced in other institutions. Improvements in faculty development have been fairly observed in the institution, exemplified by the participation of a growing number of faculty members in programs for professional development and also by the creation of a local masters degree in health education. Alongside, strong student engagement in curriculum matters enhanced the teaching-learning process. Due to a deeper involvement of students and teachers in medical education, it has been possible to implement innovative teaching-learning and assessment strategies over the last ten years and place UFRN Medical School at a privileged position in relation to undergraduate training, educational research and professional development of faculty staff.

  16. [Community perception on the quality of public health services delivery in the Norte and Ichilo districts of Bolivia].

    PubMed

    Gattinara, B C; Lbacache, J; Puente, C T; Giaconi, J; Caprara, A

    1995-01-01

    The current study identifies the people perception on the quality of public health services in Norte and Ichilo health districts (Bolivia), with a particular emphasis on the relationship between heath workers and patients. Both qualitative and quantitative methods were utilised such as: survey, focus groups, participatory observation, unstructured interviews to Key informants and open discussions with the community. Results show that the public health services reputation is affected by elements as discriminatory attitude towards people belonging to a low socio-economic strata, lack of communication between health workers and patients, perceived insufficient technical skills and recognition of a low therapeutic efficacy. The people who are coming from highlands (collas) appear as the most disappointed by the health service provision structured on the western predominant pattern with a top-bottom approach ignoring its potential users' expectations and needs. A low capability of the public health services to accept and recognise the inter-cultural features of the region, makes that the challenge to be undertaken by the district health personnel should focus on the establishment of intercultural bridges between the public health service providers and the potential patients, as well as among the different co-existing medical systems.

  17. Occurrence of anti-Toxoplasma gondii antibodies in meat and dairy goat herds in Rio Grande do Norte, Brazil.

    PubMed

    Medeiros, Andréa Dantas de; Andrade, Milena de Medeiros Clementino; Vítor, Ricardo Wagner de Almeida; Andrade-Neto, Valter Ferreira de

    2014-01-01

    Toxoplasmosis is caused by Toxoplasma gondii, which is the main causative agent of abortion in small ruminants. Goats are among the animals that are most susceptible to this protozoon, and the disease that it causes leads to significant economic losses and has implications for public health, since presence of the parasite in products of goat origin is one of the main sources of human infection. Because of the significant economic impact, there is an urgent need to study the prevalence of T. gondii infection among goats in Sertão do Cabugi, which is the largest goat-producing region in Rio Grande do Norte. In the present study, the ELISA assay was used to test 244 serum samples from nine farms, located in four different municipalities in the Sertão do Cabugi region, which is an important goat-rearing region. The results showed that the prevalence of anti-T. gondii antibodies was 47.1% and that there was a significant association between positivity and the variables of age (≥ 34 months), location (Lajes, Angicos and Afonso Bezerra) and farm (all the farms). The avidity test was applied to all the 115 ELISA-positive samples to distinguish between acute and chronic infection. One hundred and three samples (89.6%) displayed high-avidity antibodies, thus indicating that most of the animals presented chronic infection, with a consequent great impact on the development of the goat production system and a risk to human health.

  18. Copper uptake by Pteris melanocaulon Fée from a Copper-Gold mine in Surigao del Norte, Philippines.

    PubMed

    De la Torre, Joseph Benjamin B; Claveria, Rene Juna R; Perez, Rubee Ellaine C; Perez, Teresita R; Doronila, Augustine I

    2016-01-01

    The ability of some plants to take up metal contaminants in the soil has been of increasing interest as an environmental approach to pollution clean-up. This study aimed to assess the ability of Pteris melanocaulon for copper(Cu) uptake by determining the Cu levels in the fern vis-à-vis surrounding soil and the location of Cu accumulation within its biomass. It also aimed to add information to existing literature as P. melanocaulon are found to be less documented compared to other fern metal accumulators, such as P. vittata. The P. melanocaulon found in the Suyoc Pit of a Copper-Gold mine in Placer, Surigao del Norte, Philippines exhibited a high Bioaccumulation Factor(BF) of 4.04 and a low Translocation Factor(TF) of 0.01, suggesting more Cu accumulation in the roots (4590.22 ± 385.66 µg g(-1) Cu). Noteworthy was the Cu concentration in the rhizome which was also high (3539.44 ± 1696.35 µg g(-1) Cu). SEM/EDX analyses of the Cu content in the roots indicated high elemental %Cu in the xylem (6.95%) than in the cortex (2.68%). The high Cu content in the roots and rhizomes and the localization of Cu in the xylem manifested a potential utilization of the fern as a metallophyte for rhizofiltration and phytostabilization.

  19. Capacitation in the presence of methyl-β-cyclodextrin results in enhanced zona pellucida-binding ability of stallion spermatozoa.

    PubMed

    Bromfield, Elizabeth G; Aitken, R John; Gibb, Zamira; Lambourne, Sarah R; Nixon, Brett

    2014-02-01

    While IVF has been widely successful in many domesticated species, the development of a robust IVF system for the horse remains an elusive and highly valued goal. A major impediment to the development of equine IVF is the fact that optimised conditions for the capacitation of equine spermatozoa are yet to be developed. Conversely, it is known that stallion spermatozoa are particularly susceptible to damage arising as a consequence of capacitation-like changes induced prematurely in response to semen handling and transport conditions. To address these limitations, this study sought to develop an effective system to both suppress and promote the in vitro capacitation of stallion spermatozoa. Our data indicated that the latter could be achieved in a bicarbonate-rich medium supplemented with a phosphodiesterase inhibitor, a cyclic AMP analogue, and methyl-β-cyclodextrin, an efficient cholesterol-withdrawing agent. The populations of spermatozoa generated under these conditions displayed a number of hallmarks of capacitation, including elevated levels of tyrosine phosphorylation, a reorganisation of the plasma membrane leading to lipid raft coalescence in the peri-acrosomal region of the sperm head, and a dramatic increase in their ability to interact with heterologous bovine zona pellucida (ZP) and undergo agonist-induced acrosomal exocytosis. Furthermore, this functional transformation was effectively suppressed in media devoid of bicarbonate. Collectively, these results highlight the importance of efficient cholesterol removal in priming stallion spermatozoa for ZP binding in vitro.

  20. Rapidly evolving zona pellucida domain proteins are a major component of the vitelline envelope of abalone eggs

    PubMed Central

    Aagaard, Jan E.; Yi, Xianhua; MacCoss, Michael J.; Swanson, Willie J.

    2006-01-01

    Proteins harboring a zona pellucida (ZP) domain are prominent components of vertebrate egg coats. Although less well characterized, the egg coat of the non-vertebrate marine gastropod abalone (Haliotis spp.) is also known to contain a ZP domain protein, raising the possibility of a common molecular basis of metazoan egg coat structures. Egg coat proteins from vertebrate as well as non-vertebrate taxa have been shown to evolve under positive selection. Studied most extensively in the abalone system, coevolution between adaptively diverging egg coat and sperm proteins may contribute to the rapid development of reproductive isolation. Thus, identifying the pattern of evolution among egg coat proteins is important in understanding the role these genes may play in the speciation process. The purpose of the present study is to characterize the constituent proteins of the egg coat [vitelline envelope (VE)] of abalone eggs and to provide preliminary evidence regarding how selection has acted on VE proteins during abalone evolution. A proteomic approach is used to match tandem mass spectra of peptides from purified VE proteins with abalone ovary EST sequences, identifying 9 of 10 ZP domain proteins as components of the VE. Maximum likelihood models of codon evolution suggest positive selection has acted among a subset of amino acids for 6 of these genes. This work provides further evidence of the prominence of ZP proteins as constituents of the egg coat, as well as the prominent role of positive selection in diversification of these reproductive proteins. PMID:17085584

  1. Conserved developmental expression of Fezf in chordates and Drosophila and the origin of the Zona Limitans Intrathalamica (ZLI) brain organizer

    PubMed Central

    2010-01-01

    Background The zona limitans intrathalamica (ZLI) and the isthmus organizer (IsO) are two major secondary organizers of vertebrate brain development. These organizers are located at the interface of the expression domains of key patterning genes (Fezf-Irx and Otx-Gbx, respectively). To gain insights into the evolutionary origin of the ZLI, we studied Fezf in bilaterians. Results In this paper, we identified a conserved sequence motif (Fezf box) in all bilaterians. We report the expression pattern of Fezf in amphioxus and Drosophila and compare it with those of Gbx, Otx and Irx. We found that the relative expression patterns of these genes in vertebrates are fully conserved in amphioxus and flies, indicating that the genetic subdivisions defining the location of both secondary organizers in early vertebrate brain development were probably present in the last common ancestor of extant bilaterians. However, in contrast to vertebrates, we found that Irx-defective flies do not show an affected Fezf expression pattern. Conclusions The absence of expression of the corresponding morphogens from cells at these conserved genetic boundaries in invertebrates suggests that the organizing properties might have evolved specifically in the vertebrate lineage by the recruitment of key morphogens to these conserved genetic locations. PMID:20849572

  2. Inhibition of endothelin- and phorbol ester-stimulated tyrosine kinase activity by corticotrophin in the rat adrenal zona glomerulosa.

    PubMed Central

    Kapas, S; Hinson, J P

    1996-01-01

    1. The experiments described in this study were carried out to investigate the role of tyrosine kinase in the acute adrenal response to peptide hormone stimulation, and to determine whether the activity of this kinase may be subject to regulation by other intracellular signalling mechanisms in the adrenal zona glomerulosa. 2. Previous studies from this laboratory have shown that angiotensin II stimulates tyrosine kinase activity in the rat adrenal cortex. This study has shown, for the first time, that endothelin-1 also stimulates tyrosine kinase activity in this tissue. 3. Using the specific inhibitor of protein kinase C (PKC) activity, Ro 31-8220, we have shown that stimulation of tyrosine kinase activity, in response to endothelin-1, angiotensin II or the phorbol ester phorbol 12-myristate 13-acetate, is at least partly dependent on increased PKC activity. 4. The data presented also provide further evidence of cross-talk between signalling systems in the adrenal cortex. Corticotrophin and its intracellular second messenger, cyclic AMP, significantly attenuate the increment in tyrosine kinase activity seen in response to each of the effectors used. 5. The results of this study provide important new evidence for the regulation of protein kinases by other intracellular second messenger systems. PMID:8611168

  3. Pertussis toxin treatment does not block inhibition by atrial natriuretic factor of aldosterone secretion in cultured bovine zona glomerulosa cells

    SciTech Connect

    De Lean, A.; Cantin, M.

    1986-03-05

    The authors have previously reported that atrial natriuretic factor (ANF) potently inhibits PGE or forskolin-stimulation aldosterone secretion in bovine zona glomerulosa (ZG) by acting through specific high affinity receptors. In order to evaluate the functional role of the regulatory protein N/sub i/ and the inhibition of adenylate cyclase activity (AC) in ZG, the authors have studied the effect of treatment with PT on inhibition by ANF of aldosterone production. Primary cultures of ZG were treated for 18 hours in serum-free F12 medium with (0-100 ng/ml PT). No effect of PT pretreatment was observed either on basal, PGE-stimulated or ANF-inhibited levelsmore » of steroidogenesis. When membranes prepared from control ZG were ADP-ribosylated with (/sup 32/P) NAD in the presence of PT, two toxin-specific bands with 39 Kd and 41 Kd were documented on SDS gel. Cell pretreatment with as low as 1 ng/ml drastically reduced further labelling of these two bands while higher doses completely abolished them. Since PT treatment covalently modifies completely the toxin substrate without altering ANF inhibition of adrenal steroidogenesis, the authors conclude that N/sub i/ is not involved in the mode of action of ANF on aldosterone production.« less

  4. Human spermatozoa selected by Percoll gradient or swim-up are equally capable of binding to the human zona pellucida and undergoing the acrosome reaction.

    PubMed

    Morales, P; Vantman, D; Barros, C; Vigil, P

    1991-03-01

    Several techniques have been used for selecting motile spermatozoa including Percoll and albumin gradients, swim-up, and glass wool filtration. A high yield of motile spermatozoa as well as an enhancement of motility are the most desirable features of a practical method. An equally important consideration is whether or not these techniques select functionally normal spermatozoa. In this study we have compared two methods for separation of motile cells, swim-up and Percoll gradient. Normal semen samples from 12 different men were used in this study. Each sample was simultaneously processed by swim-up and Percoll gradient using modified Tyrode's medium. After the sperm concentration was adjusted to 1 x 10(7) spermatozoa/ml, the suspensions were incubated at 37 degrees C, 5% CO2 in air. In each suspension the percentage of sperm recovery, percentage of motile spermatozoa, percentage of acrosome reacted spermatozoa (either spontaneously or stimulated with human follicular fluid), percentage of zona-free hamster oocytes penetrated, and number of spermatozoa bound to the human zona pellucida were determined. The results obtained indicated that the percentage of sperm recovery was higher with the Percoll gradient than with the swim-up procedure (P less than 0.001). However, no significant differences were found between these two sperm populations in the percentage of motile cells, in the percentage of acrosome reacted spermatozoa, and in the percentage of zona-free hamster oocytes penetrated. In addition, the number of spermatozoa bound per zona pellucida was similar for spermatozoa selected by Percoll or swim-up. We conclude that there were no functional differences between the spermatozoa selected by either method.

  5. Time-lapse monitoring of zona pellucida-free embryos obtained through in vitro fertilization: a retrospective case series.

    PubMed

    Bodri, Daniel; Kato, Ryutaro; Kondo, Masae; Hosomi, Naoko; Katsumata, Yoshinari; Kawachiya, Satoshi; Matsumoto, Tsunekazu

    2015-05-01

    To report time-lapse monitoring of human oocytes in which the damaged zona pellucida was removed, producing zona-free (ZF) oocytes that were cultured until the blastocyst stage in time-lapse incubators. Retrospective case series. Private infertility clinic. Infertile patients (n = 32) undergoing minimal ovarian stimulation or natural cycle IVF treatment between October 2012 and June 2014. Intracytoplasmic sperm injection (ICSI) fertilization of ZF oocytes, prolonged embryo culture in time-lapse incubators, elective vitrification, and subsequent single vitrified-thawed blastocyst transfer (SVBT). Rate of fertilization, cleavage and blastocyst development, live-birth rate per SVBT cycle. In spite of advanced maternal age (39 ± 4.2; range, 30-46 years), good fertilization (94%), cleavage (94%), and blastocyst development rates (38%) were reached after fertilization and culturing of ZF oocytes/embryos. All thawed ZF blastocysts survived, and up to this date seven SVBT transfers were performed, yielding three (43%) term live births with healthy newborns. Time-lapse imagery gives a unique insight into the dynamics of embryo development in ZF embryos. Moreover, our case series demonstrate that an oocyte with a damaged zona pellucida that has been removed could be successfully fertilized with ICSI, cultured until blastocyst stage in a time-lapse incubator and vitrified electively for subsequent use. Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  6. Morbidity, utilization of curative care and service entry point preferences in metropolitan Centro Habana, Cuba.

    PubMed

    Rodríguez, Armando; Díaz, Addys; Balcindes, Susana; García, René; Vos, Pol De; Stuyft, Patrick Van der

    2016-11-03

    First-line health services with a primary health care approach are a strong trigger for adequate health-care-seeking behavior. Research on the association between prevalence of chronic diseases and acute illnesses and use of health services emphasizes the importance of socioeconomic determinants in such patterns of utilization. In a cross-sectional study of 408 families in Centro Habana, Cuba, home interviews were conducted between April and June 2010 to analyze socio-demographic determinants of acute and chronic health problems and use of formal health services. Bivariate and logistic regression models were used. 529 persons reported a chronic disease. During the previous month, 155 of the latter reported an exacerbation and 50 experienced an unrelated acute health problem. 107 persons without chronic diseases reported acute health problems. Age was the strongest determinant of chronic disease prevalence. Adult women and the elderly were more likely to report acute problems. Acute patients with underlying chronic disease used formal services more often. No socio-demographic variable was associated with services use or consultation with the family physician. While the family physician is defined as the system's entry-point, this was the case for only 54% of patients that had used formal services, thus compromising the physician's role in counseling patients and summarizing their health issues. The importance of chronic diseases highlights the need to strengthen the family physician's pivotal role. New economic policies in Cuba, stimulating self-employment and private initiative, may increase the strain on the exclusively public health care system. Still, the Cuban health system has demonstrated its ability to adapt to new challenges, and the basic premises of Cuba's health policy are expected to be preserved.

  7. [Food habits of the school population from La Mancha-Centro Health Area (Ciudad Real)].

    PubMed

    González-González, A; Falero-Gallego, M P; Redondo-González, O; Muñoz-Serrano, A

    2016-03-01

    To assess the dietary pattern of the school population from La Mancha-Centro Health Area (Ciudad Real). A cross-sectional study conducted on a representative sample of schoolchildren aged 6 to 12 years, using a questionnaire to determine their general dietary habits and a record of their 24-hour intake. A total of 1142 schoolchildren were included, with 612 boys (53.6%) and 530 girls (46.4%), and a mean age of 9.3 ± 1.7 years of age. The frequency of food intake was 4.62 ± 0.6 times a day, which decreased with the age of the schoolchildren (P=.044), and increased with the educational level of parents (P=.004). Food preference influenced the choice in the meals consumed. The level of appetite was related directly with weight and body mass index (BMI) (P<.001), age (P=.02), and number of daily food intakes by the children (P=.038). The food groups most frequently consumed were cereals and their derivatives (92.8%), milk and dairy products (90.45%), while vegetables were the least consumed (35.46%). Over 70% of the sample usually consumed olive oil. The dietary pattern of the school population maintains some of the features of traditional Mediterranean dietary pattern, such as the habit of daily breakfast, the greater consumption of olive oil and cereals. On the other hand, other characteristics are remarkable, such as the low consumption of fruit and vegetables and the high consumption of meat. Copyright © 2015 Asociación Española de Pediatría. Published by Elsevier España, S.L.U. All rights reserved.

  8. Fluctuations of Glaciar Esperanza Norte in the North Patagonian Andes of Argentina during the past 400 yr

    NASA Astrophysics Data System (ADS)

    Ruiz, L.; Masiokas, M. H.; Villalba, R.

    2011-11-01

    The number of studies of Little Ice Age (LIA) glacier fluctuations in Southern South America has increased in recent years but is largely biased towards sites in the South Patagonian Andes. In this paper we present a detailed record of length and areal fluctuations of Glaciar Esperanza Norte (GEN), in the North Patagonian Andes of Argentina, during the past four centuries. The GEN record was reconstructed through the dendro-geomorphological dating of moraines and the analysis of satellite imagery, aerial photographs and documentary material complemented with extensive field surveys. The maximum LIA extent at GEN was associated with an outer moraine dated to the mid 17th century. At least 19 subsequent readvances or standstills evidenced by morainic ridges were identified inside the most extensive LIA moraine. The dating and spacing of these moraines and the additional information available indicate that the ice front retreated much more rapidly during the 20th century than during earlier centuries. Comparison with the record of LIA fluctuations of Glaciar Frías, an ice mass of similar characteristics located 110 km to the north of GEN, shows a similar pattern of recession over the past 400 yr. Both glacier records have the peak LIA event occurring roughly during the same interval and show a minor readvance during the 1970s, but there are still a few discrepancies in the dating of some inner moraines. These differences may be due to local, specific factors or associated with the inherent uncertainties in the dating of the moraines. The chronologies of GEN and Frías are among the most detailed currently available in Patagonia, but a larger number of study sites is needed to develop robust, regionally representative glacier chronologies. Detailed glaciological, geomorphological and meteorological data are also needed to understand the glacier-climate relationships in this region and develop reliable paleoclimatic reconstructions.

  9. Fluctuations of Glaciar Esperanza Norte in the north Patagonian Andes of Argentina during the past 400 yr

    NASA Astrophysics Data System (ADS)

    Ruiz, L.; Masiokas, M. H.; Villalba, R.

    2012-06-01

    The number of studies of Little Ice Age (LIA) glacier fluctuations in southern South America has increased in recent years but is largely biased towards sites in the south Patagonian Andes. In this paper we present a detailed record of length and areal fluctuations of Glaciar Esperanza Norte (GEN) in the north Patagonian Andes of Argentina during the past four centuries. The GEN record was reconstructed through the dendro-geomorphological dating of moraines and the analysis of satellite imagery, aerial photographs and documentary material complemented with extensive field surveys. The maximum LIA extent at GEN was associated with an outer moraine dated to the mid 17th century. At least 19 subsequent readvances or standstills evidenced by morainic ridges were identified inside the most extensive LIA moraine. The dating and spacing of these moraines and the additional information available indicate that the ice front retreated much more rapidly during the 20th century than during earlier centuries. Comparison with the record of LIA fluctuations of Glaciar Frías, an ice mass of similar characteristics located 110 km to the north of GEN, shows a similar pattern of recession over the past 400 yr. Both glacier records have the peak LIA event occurring roughly during the same interval (early-mid 17th century) and show a minor readvance during the 1970s, but there are still a few discrepancies in the dating of some inner moraines. These differences may be due to local, specific factors or associated with the inherent uncertainties in the dating of the moraines. The chronologies of GEN and Frías are among the most detailed currently available in Patagonia, but a larger number of study sites is needed to develop robust, regionally representative glacier chronologies. Detailed glaciological, geomorphological and meteorological data are also needed to understand the glacier-climate relationships in this region and develop reliable paleoclimatic reconstructions.

  10. Ability of spermatozoa to bind to the zona pellucida during oligozoospermia induced with testosterone during a male contraceptive trial.

    PubMed

    Liu, D Y; Johnston, R; Baker, H W

    1995-06-01

    To determine the ability of spermatozoa to bind to the zona pellucida (ZP) in testosterone-induced oligozoospermia, previously fertile men participating in the World Heath Organization (WHO) male contraceptive trial in Melbourne were studied while oligozoospermic to various degrees. Semen analysis were performed according to WHO methods. One or two ejaculates from each subject were cryopreserved before commencing weekly intramuscular injections of 200 mg testosterone enanthate. The frozen spermatozoa were used as controls for ZP-binding tests of spermatozoa obtained during testosterone-induced oligozoospermia (< 10 x 10(6)/ml) in either the suppression or efficacy (n = 6) and recovery (n = 3) phases. Two other subjects in the recovery phase with normozoospermia were also tested. Human oocytes that failed to fertilized in vitro from infertile patients were used for the sperm-ZP binding test. Control (frozen) spermatozoa were labelled with fluorescein isothiocyanate and test (oligozoospermic semen) spermatozoa were labelled with tetramethylrhodamine B isothiocyanate. A mixture of equal numbers of labelled motile control and test spermatozoa were incubated with 4-6 ZP. There was a significantly (p < 0.01) lower number of spermatozoa bound per ZP in oligozoospermic samples (65 +/- 7, mean +/- SEM) than in controls (80 +/- 7). However, there were still large numbers of spermatozoa bound to the ZP for all the oligozoospermic samples. Five subjects had similar numbers of spermatozoa bound to the ZP for both control and oligozoospermic samples. Overall, the ZP-binding ratio of test and control spermatozoa averaged 0.82 (range 0.51-1.13).(ABSTRACT TRUNCATED AT 250 WORDS)

  11. Uncontacted Waorani in the Yasuní Biosphere Reserve: Geographical Validation of the Zona Intangible Tagaeri Taromenane (ZITT)

    PubMed Central

    Pappalardo, Salvatore Eugenio; De Marchi, Massimo; Ferrarese, Francesco

    2013-01-01

    The Tagaeri Taromenane People are two indigenous groups belonging to the Waorani first nation living in voluntary isolation within the Napo region of the western Amazon rainforest. To protect their territory the Ecuadorean State has declared and geographically defined, by Decrees, the Zona Intangible Tagaeri Taromenane (ZITT). This zone is located within the UNESCO Yasuní Biosphere Reserve (1989), one of the most biodiverse areas in the world. Due to several hydrocarbon reserve exploitation projects running in the area and the advancing of a large-scale deforestation front, the survival of these groups is presently at risk. The general aim was to validate the ZITT boundary using the geographical references included in the Decree 2187 (2007) by analyzing the geomorphological characteristics of the area. Remote sensing data such as Digital Elevation Models (DEM), Landsat imagery, topographic cartography of IGM-Ecuador, and fieldwork geographical data have been integrated and processed by Geographical Information System (GIS). The ZITT presents two levels of geographic inconsistencies. The first dimension is about the serious cartographical weaknesses in the perimeter delimitation related to the impossibility of linking two rivers belonging to different basins while the second deals with the perimeter line not respecting the hydrographic network. The GIS analysis results clearly show that ZITT boundary is cartographically nonsense due to the impossibility of mapping out the perimeter. Furthermore, GIS analysis of anthropological data shows presence of Tagaeri Taromenane clans outside the ZITT perimeter, within oil production areas and in nearby farmer settlements, reflecting the limits of protection policies for non-contacted indigenous territory. The delimitation of the ZITT followed a traditional pattern of geometric boundary not taking into account the nomadic characteristic of Tagaeri Taromenane: it is necessary to adopt geographical approaches to recognize the

  12. Effects of milk proteins on sperm binding to the zona pellucida and intracellular Ca(2+) concentration in stallion sperm.

    PubMed

    Coutinho da Silva, Marco A; Seidel, George E; Squires, Edward L; Graham, James K; Carnevale, Elaine M

    2014-11-10

    Objectives were to determine the effects of extracellular Ca(2+) and milk proteins on intracellular Ca(2+) concentrations in stallion sperm; and to determine the effects of single caseins on sperm binding to the zona pellucida (ZP). In Experiment I, sperm were incubated in media containing 2 or 4mM Ca(2+) and intracellular Ca(2+) concentration was determined after ionomycin treatment and long-term incubation (3h). Extracellular Ca(2+) concentrations (2 compared with 4mM) did not affect baseline intracellular Ca(2+) concentration of sperm. However, incubating sperm in a medium containing 4 compared with 2mM Ca(2+) resulted in greater (P<0.05) influx of Ca(2+) into sperm. In Experiment II, sperm incubated in media containing 1mg/mL of native phosphocaseinate (NP) or sodium caseinate (SC) showed similar baseline intracellular Ca(2+) and influx of Ca(2+) than control (TALP). In Experiment III, sperm-ZP binding assays were performed in TALP medium containing: no additions (TALP); 1mg/mL SC; 1 or 3mg/mL of α-casein; 1 or 3mg/mL of β-casein; and 1 or 3mg/mL of κ-casein. The number of stallion sperm bound to bovine ZP was greatest (P<0.05) when SC was used. Co-incubation in media containing single caseins (α-, β- or κ-casein) resulted in similar results to TALP; however, a dose effect (P<0.05) was observed for β- and κ-caseins. In conclusion, extracellular Ca(2+) concentration and milk proteins did not affect baseline intracellular calcium in stallion sperm. It appears that β- and κ-caseins may be responsible for enhancing sperm binding to ZP, but the mechanism remains unknown. Copyright © 2014 Elsevier B.V. All rights reserved.

  13. Uncontacted Waorani in the Yasuní Biosphere Reserve: Geographical Validation of the Zona Intangible Tagaeri Taromenane (ZITT).

    PubMed

    Pappalardo, Salvatore Eugenio; De Marchi, Massimo; Ferrarese, Francesco

    2013-01-01

    The Tagaeri Taromenane People are two indigenous groups belonging to the Waorani first nation living in voluntary isolation within the Napo region of the western Amazon rainforest. To protect their territory the Ecuadorean State has declared and geographically defined, by Decrees, the Zona Intangible Tagaeri Taromenane (ZITT). This zone is located within the UNESCO Yasuní Biosphere Reserve (1989), one of the most biodiverse areas in the world. Due to several hydrocarbon reserve exploitation projects running in the area and the advancing of a large-scale deforestation front, the survival of these groups is presently at risk. The general aim was to validate the ZITT boundary using the geographical references included in the Decree 2187 (2007) by analyzing the geomorphological characteristics of the area. Remote sensing data such as Digital Elevation Models (DEM), Landsat imagery, topographic cartography of IGM-Ecuador, and fieldwork geographical data have been integrated and processed by Geographical Information System (GIS). The ZITT presents two levels of geographic inconsistencies. The first dimension is about the serious cartographical weaknesses in the perimeter delimitation related to the impossibility of linking two rivers belonging to different basins while the second deals with the perimeter line not respecting the hydrographic network. The GIS analysis results clearly show that ZITT boundary is cartographically nonsense due to the impossibility of mapping out the perimeter. Furthermore, GIS analysis of anthropological data shows presence of Tagaeri Taromenane clans outside the ZITT perimeter, within oil production areas and in nearby farmer settlements, reflecting the limits of protection policies for non-contacted indigenous territory. The delimitation of the ZITT followed a traditional pattern of geometric boundary not taking into account the nomadic characteristic of Tagaeri Taromenane: it is necessary to adopt geographical approaches to recognize the

  14. Voice Tremor Outcomes of Subthalamic Nucleus and Zona Incerta Deep Brain Stimulation in Patients With Parkinson Disease.

    PubMed

    Karlsson, Fredrik; Malinova, Elin; Olofsson, Katarina; Blomstedt, Patric; Linder, Jan; Nordh, Erik

    2018-01-17

    We aimed to study the effect of deep brain stimulation (DBS) in the subthalamic nucleus (STN) and caudal zona incerta (cZi) on level of perceived voice tremor in patients with Parkinson disease (PD). This is a prospective nonrandomized design with consecutive patients. Perceived voice tremor was assessed in patients with PD having received either STN-DBS (8 patients, 5 bilateral and 3 unilateral, aged 43.1-73.6 years; median = 61.2 years) or cZi-DBS (14 bilateral patients, aged 39.0-71.9 years; median = 56.6 years) 12 months before the assessment. Sustained vowels that were produced OFF and ON stimulation (with simultaneous l-DOPA medication) were assessed perceptually in terms of voice tremor by two raters on a four-point rating scale. The assessments were repeated five times per sample and rated in a blinded and randomized procedure. Three out of the 22 patients (13%) were concluded to have voice tremor OFF stimulation. Patients with PD with STN-DBS showed mild levels of perceived voice tremor OFF stimulation and a group level improvement. Patients with moderate/severe perceived voice tremor and cZi-DBS showed marked improvements, but there was no overall group effect. Six patients with cZi-DBS showed small increases in perceived voice tremor severity. STN-DBS decreased perceived voice tremor on a group level. cZi-DBS decreased perceived voice tremor in patients with PD with moderate to severe preoperative levels of the symptom. Copyright © 2017 The Voice Foundation. Published by Elsevier Inc. All rights reserved.

  15. Centro de Divulgação da Astronomia - o Observatório de São Carlos

    NASA Astrophysics Data System (ADS)

    Dias, W. S.; Hönel, J.; Líbero, V. L.; Anderlini, A.; de Andrade, D.; Scatena, E.; Lazo, M. J.; Trevisan, M.; de Castro, V.

    2005-07-01

    Apresentamos as principais características do Centro de Divulgação da Astronomia, que é constituído e um observatório cujo objetivo é a divulgação da Astronomia junto à comunidade, através de palestras e de observação direta dos astros. Descrevemos, neste trabalho, as atividades desenvolvidas desde sua fundação em 1986, e mostramos sua importância na região através de números anuais das atividades realizadas.

  16. Attic Dust Analysis Approach for Evaluation of Heavy Metal Deposition on the El Paso Del Norte Region

    NASA Astrophysics Data System (ADS)

    Shekhter, E. G.; van Pelt, S.; Pannell, K.; Gill, T. E.; Barnes, M. A.

    2010-12-01

    The El Paso del Norte region in the United States is a region of complex topography that is the home to more than 2 million people who share the same air. A large non-ferrous smelter (primary lead-copper smelter),owned by the American Smelting and Refining Company (ASARCO), was functioning in the Rio Grande River valley on the west side of the Franklin Mountains that divide the city of El Paso and across the river from Juarez, Mexico’s largest city on the United States border. During the more than 100 years of operation, beginning in 1887, the ASARCO smelter emissions considerably deteriorated the already complex environmental condition on the US-Mexican border. In order to identify the sources and patterns of heavy metal dispersion, we used an attic dust analyses approach which has not been previously been applied in this area. Undisturbed attic dust can provide an integrated history of atmospheric loading of particulates emanating from geological, biological and anthropogenic sources and can help to reconstruct the air pollution history on a local scale. We sampled attic dust and dust from undisturbed surfaces in 15 buildings of varying ages from three neighborhoods in the cities of El Paso and Juarez. The dust samples and samples of unpolluted buried soil horizons in the area were extracted with aqua regia and analyzed by ICP-AES and ICP-MS. Enrichment ratios (ER) were calculated by dividing the concentration of a trace metal in the attic dust by the mean concentration of the same element in the buried soil horizons. Mean ER for Pb were 263.3, 95.4, and 70.2 for dust collected in a neighborhood near the smelter, downtown Juarez, and an El Paso neighborhood on the other side of the Franklin Mountains from the smelter. Greater ER for As, Cd, Zn, Sb, and Cu followed the same trends, by neighborhood, noted for Pb. In addition, dust collected from attics surfaces (pipes, ducts, storage containers) post-dating smelter disclosure had lower ER for the trace metals

  17. Fecal 20-oxo-pregnane concentrations in free-ranging African elephants (Loxodonta africana) treated with porcine zona pellucida vaccine.

    PubMed

    Ahlers, M J; Ganswindt, A; Münscher, S; Bertschinger, H J

    2012-07-01

    Because of overpopulation of African elephants in South Africa and the consequent threat to biodiversity, the need for a method of population control has become evident. In this regard, the potential use of the porcine zona pellucida (pZP) vaccine as an effective means for population control is explored. While potential effects of pZP treatment on social behavior of African elephants have been investigated, no examination of the influence of pZP vaccination on the endocrine correlates in treated females has been undertaken. In this study, ovarian activity of free-ranging, pZP-treated African elephant females was monitored noninvasively for 1 yr at Thornybush Private Nature Reserve, South Africa, by measuring fecal 5α-pregnan-3β-ol-20-on concentrations via enzyme immunoassay. A total of 719 fecal samples from 19 individuals were collected over the study period, averaging 38 samples collected per individual (minimum, maximum: 16, 52). Simultaneously, behavioral observations were made to record the occurrence of estrous behavior for comparison. Each elephant under study showed 5α-pregnan-3β-ol-20-on concentrations rising above baseline at some period during the study indicating luteal activity. Average 5α-pregnan-3β-ol-20-on concentrations were 1.61 ± 0.46 μg/g (mean ± SD). Within sampled females, 42.9% exhibited estrous cycles within the range reported for captive African elephants, 14.3% had irregular cycles, and 42.9% did not appear to be cycling. Average estrous cycle duration was 14.72 ± 0.85 wk. Estrous behavior coincided with the onset of the luteal phase and a subsequent rise in 5α-pregnan-3β-ol-20-on concentrations. Average 5α-pregnan-3β-ol-20-on levels positively correlated with rainfall. No association between average individual 5α-pregnan-3β-ol-20-on concentrations or cyclicity status with age or parity were detected. Earlier determination of efficacy was established via fecal hormone analysis with no pregnancies determined 22 mo post

  18. Inoculation of female American black bears (Ursus americanus) with partially purified porcine zona pellucidae limits cub production.

    PubMed

    Lane, V M; Liu, I K M; Casey, K; vanLeeuwen, E M G; Flanagan, D R; Murata, K; Munro, C

    2007-01-01

    The present 2-year study investigated the feasibility of using porcine zona pellucidae (pZP) as antigen for immunocontraception in American black bears. Sows, 3-6 years of age, were administered either two doses of 250 microg pZP with Freund's adjuvant (n = 10) or adjuvant alone (n = 5), one in April and one in May, and were kept away from the boars until June. Serum samples were collected before injections and before denning (November). The presence of sows with cubs at side was observed during premature emergence from denning. First-year results indicated that anti-pZP antibody titres in vaccinated sows were 2.5-9.0-fold (range) higher compared with non-vaccinated sows and that the vaccinated sows were threefold less likely to become pregnant (P = 0.167). Control and vaccinated bears produced 1.6 and 0.2 cubs per sow, respectively (P = 0.06). The second-year study investigated the feasibility of using pZP sequestered in a controlled-release pellet and a water-soluble adjuvant (QS-21) to avoid regulatory problems associated with Freund's adjuvant. Sows in the treatment group (n = 22) were administered a single dose of an emulsion of 250 microg pZP and 150 microg QS-21 plus a pellet containing 70-90 microg pZP for delayed release as booster dose. Control sows (n = 5) received the QS-21 adjuvant in pellet alone. Serum samples were collected before inoculations (April) and before denning (November). Seven cubs were born to the five control sows, but none was born to the 22 vaccinated sows (P < 0.001). Anti-pZP antibody mean absorbance ratios in control sows remained at background levels, whereas vaccinated sows had ratios fourfold higher than controls. Two-dimensional polyacrylamide gel electrophoresis and immunohistochemical localisation confirmed immunoreactivity of sera from inoculated bears. We conclude that cub production in the American black bear can be effectively limited with either two injections of 250 microg pZP or a single inoculation of partially

  19. Do age and extended culture affect the architecture of the zona pellucida of human oocytes and embryos?

    PubMed

    Kilani, Suha S; Cooke, Simon; Kan, Andrew K; Chapman, Michael G

    2006-02-01

    Advanced female age and extended in vitro culture have both been implicated in zona pellucida (ZP) hardening and thickening. This study aimed to determine the influence of (i) the woman's age and (ii) prolonged in vitro culture of embryos on ZP thickness and density using non-invasive polarized light (LC-PolScope) microscopy. ZP thickness and density (measured as retardance) were determined in oocytes, embryos and blastocysts in women undergoing intracytoplasmic sperm injection (ICSI) in two age groups (older, > 38 years; younger, < or = 38 years). A total of 193 oocytes from 29 patients were studied. The younger group contained 100 oocytes and the older group 93 oocytes. The ZP was significantly thicker in metaphase II oocytes in the older group compared with the younger group (mean +/- SD: 24.1 +/- 2.5 microm vs 23.1 +/- 3.3 microm; p = 0.01) but ZP density was equal (2.8 +/- 0.7 nm). By day 2 of culture, embryos from the two groups had similar ZP thickness (22.2 +/- 2.2 microm vs 21.7 +/- 1.6 microm; p = 0.28) and density (2.9 +/- 0.7 nm vs 2.8 +/- 0.8 nm; p = 0.57). For the embryos cultured to blastocyst (older: n = 20; younger: n = 18) ZP thickness was similar in the two groups (19.2 +/- 2.7 microm vs 19.1 +/- 5.0 microm; p = 0.8) but thinner than on day 2. The older group had significantly denser ZP than the younger group (4.2 +/- 0.5 nm vs 3.3 +/- 1.0 nm, p < 0.01). Blastocysts from both groups had significantly denser ZP than their corresponding day 2 embryos (older: 4.2 +/- 0.5 nm vs 2.9 +/- 0.7 nm, p < 0.001; younger: 3.3 +/- 1.0 nm vs 2.8 +/- 0.8 nm, p = 0.013). It is concluded that there is little relationship between ZP thickness and its density as measured by polarized light microscopy. While ZP thickness decreases with extended embryo culturing, the density of the ZP increases. ZP density increases in both age groups with extended culture and, interestingly, more in embryos from older compared with younger women.

  20. The preclinical evaluation of immunocontraceptive vaccines based on canine zona pellucida 3 (cZP3) in a mouse model.

    PubMed

    Wang, Ying; Li, Yijie; Zhang, Beibei; Zhang, Fuchun

    2018-05-11

    Stray dogs are the reservoirs and carriers of rabies and are definitive hosts of echinococcosis. To control the overpopulation of stray dogs, zona pellucida 3 (ZP3), a primary receptor for sperm, is a potential antigen for developing contraceptive vaccines. To enhance the immune responses and contraceptive effects of canine ZP3 (cZP3), dog gonadotropin-releasing hormone (GnRH) and a T cell epitope of chicken ovalbumin (OVA) were selected to construct two fusion proteins with cZP3, ovalbumin-GnRH-ZP3 (OGZ) and ovalbumin-ZP3 (OZ), and their contraceptive effects were evaluated in mice. The synthesized DNA sequences of OGZ and OZ were cloned into plasmid pET-28a respectively. The fusion proteins OGZ and OZ were identified by SDS-PAGE and Western blot. Mice were immunized with OGZ, OZ and cZP3, and the infertility rates were monitored. Mice immunized with mouse ZP3 (mZP3) or adjuvant alone were used as positive control and negative control, respectively. cZP3- and GnRH-specific antibodies (Abs) were detected by ELISA. The bindings of the Abs to oocytes were detected by indirect immunofluorescence assay. The paraffin sections of mice ovaries were observed under microscope for analyzing pathological characteristics. SDS-PAGE and Western blot analyses showed that the two fusion proteins OGZ and OZ were correctly expressed. ELISA results showed that OGZ vaccine induced both cZP3- and GnRH-specific Abs, and OZ vaccine induced cZP3-specific Ab, which lasted for up to 168 days. The levels of follicle stimulating hormone (FSH) and estradiol (E2) in sera were significantly decreased in OGZ immunized mice. Indirect immunofluorescence results showed that Abs induced by cZP3 and mZP3 could bind to the mouse ZP and dog ZP each other. Compared with the adjuvant group, all vaccine immunized groups significantly decreased the fertility rate and mean litter size. Interestingly, the fertility rate in OGZ-immunized group is the lowest, and only 1 mouse out of 10 mice is fertile

  1. Single-treatment porcine zona pellucida immunocontraception associated with reduction of a population of white-tailed deer (Odocoileus virginianus).

    PubMed

    Rutberg, Allen T; Naugle, Ricky E; Verret, Frank

    2013-12-01

    Previous reports have demonstrated gradual reductions of white-tailed deer (Odocoileus virginianus) populations through immunocontraception, with stabilization occurring after 2-4 yr of treatment, and subsequent reductions of 6-10% annually. These studies employed porcine zona pellucida (PZP) vaccines that required two initial treatments and annual retreatments. From 2005 to 2010, 258 adult and yearling female deer on Fripp Island, South Carolina, were treated with one of several PZP preparations designed to produce 2+ yr of effective contraception with a single treatment. These included several preparations of SpayVac and of native PZP-adjuvant emulsion plus PZP and QA-21 in timed-release pellets. Deer were chemically immobilized, ear-tagged, and administered initial treatments by hand in February-March. Some treated deer were boosted remotely with PZP-adjuvant emulsion 1.5 - 4.5 yr after initial treatments. Ground-based distance sampling was used to estimate deer population density at Fripp Island, a resort community, and at a relatively undeveloped neighboring control site, Hunting Island. Most vaccine preparations tested reduced fawning rates by 75% to 95% for at least 1 yr. From 2005 to 2011, deer density on Fripp Island declined by 50%, from 72 deer/km(2) to 36 deer/km(2), an average annual reduction of 11%. In contrast, population density on the Hunting Island control site fluctuated between 2005 and 2011, averaging 23 deer/km(2) (range, 19-28 deer/km(2)). Population declines on Fripp Island were associated with an increase in the proportion of treated females and with a progressive decrease in winter fawn:doe ratios, from 1.21 fawns/doe in 2005 to 0.19 fawns/doe in 2010. Winter fawn:doe ratios averaged 1.36 fawns/doe (range, 0.84 - 1.62 fawns/doe) at the Hunting Island control site. Annual survivorship averaged approximately 79% among ear-tagged females. The rate at which deer populations diminished in association with PZP treatments on Fripp Island was

  2. Use of Both Cumulus Cells’ Transcriptomic Markers and Zona Pellucida Birefringence to Select Developmentally Competent Oocytes in Human Assisted Reproductive Technologies

    PubMed Central

    2015-01-01

    Background Selection of the best oocyte for subsequent steps of fertilization and embryo transfer was shown to be the crucial step in human infertility treatment procedure. Oocyte selection using morphological criteria mainly Zona pellucida (ZP) has been the gold standard method in assisted reproductive technologies (ART) clinics, but this selection approach has limitations in terms of accuracy, objectivity and constancy. Recent studies using OMICs-based approaches have allowed the identification of key molecular markers that quantitatively and non-invasively predict the oocyte quality for higher pregnancy rates and efficient infertility treatment. These biomarkers are a valuable reinforcement of the morphological selection criteria widely used in in vitro fertilization (IVF) clinics. In this context, this study was designed to investigate the relationship between transcriptomic predictors of oocyte quality found by our group and the conventional morphological parameters of oocyte quality mainly the ZP birefringence. Results Microarray data revealed that 48 and 27 differentially expressed candidate genes in cumulus cells (CCs) were respectively overexpressed and underexpressed in the ZGP (Zona Good Pregnant) versus ZBNP (Zona Bad Non Pregnant) groups. More than 70% of previously reported transcriptomic biomarkers of oocyte developmental competence were confirmed in this study. The analysis of possible association between ZP birefringence versus molecular markers approach showed an absence of correlation between them using the current set of markers. Conclusions This study suggested a new integrative approach that matches morphological and molecular approaches used to select developmentally competent oocytes able to lead to successful pregnancy and the delivery of healthy baby. For each ZP birefringence score, oocytes displayed a particular CCs' gene expression pattern. However, no correlations were found between the 7 gene biomarkers of oocyte developmental

  3. Análisis de los determinantes socioeconómicos del gasto de bolsillo en medicamentos en seis zonas geográficas de Panamá.

    PubMed

    Herrera-Ballesteros, Victor H; Castro, Franz; Gómez, Beatriz

    2018-04-27

    Caracterizar el gasto de bolsillo privado en medicamentos en función de los determinantes sociodemográficos y socioeconómicos. MATERIALES Y MéTODOS: La fuente de datos es la Encuesta de Gasto de Bolsillo en Medicamentos de 2014. Se caracterizó el gasto de bolsillo privado mediante variables explicativas sociodemográficas (SOD) y socioeconómicas (SES). Se hizo análisis factorial por componentes principales, regresión logística y lineal simple. Los Odds Ratio demuestran que la educación y la zona geográfica son determinantes fundamentales que inciden en el gasto de bolsillo. Los medicamentos son productos necesarios, en adición a que el gasto de bolsillo aumenta a un promedio del 2% por cada año de vida cronológica adicional. Existe mayor vulnerabilidad en las zonas más pauperizadas respecto del acceso a medicamentos, en especial en las indígenas e implica un mayor riesgo de gasto catastrófico a menor ingreso ante la mayor prevalencia de enfermedades crónicas. Copyright © 2018. Published by Elsevier Inc.

  4. Angio-OCT de la zona avascular foveal en ojos con oclusión venosa de la retina.

    PubMed

    Wons, Juliana; Pfau, Maximilian; Wirth, Magdalena A; Freiberg, Florentina J; Becker, Matthias D; Michels, Stephan

    2017-07-11

    Objetivo: El objetivo del estudio comprendía visualizar y cuantificar las alteraciones patológicas de la zona avascular foveal (ZAF) mediante angio-OCT en ojos con oclusión venosa de la retina (OVR) en comparación con el ojo contralateral sano. Procedimientos: La angio-OCT se llevó a cabo mediante el sistema Avanti® RTVue 100 XR (Optovue Inc., Fremont, Calif., EE. UU.). Los bordes de la capa vascular superficial (CVS) se definieron como 3 μm por debajo de la membrana limitante interna y 15 μm por debajo de la capa plexiforme interna y, para la capa vascular profunda (CVP), como 15 y 70 μm por debajo de la membrana limitante interna y de la capa plexiforme interna, respectivamente. La longitud de la ZAF horizontal, vertical y máxima de la CVS y la CVP en cada ojo se midió de forma manual. Además, se midió el ángulo entre el diámetro máximo de la ZAF y el plano papilomacular. Resultados: La angio-OCT representó los defectos dentro de la vasculatura en el área perifoveal en ojos con oclusión de rama venosa de la retina (ORVR; n = 11) y con oclusión de la vena central de la retina (OVCR; n = 8). Esto resultó en un crecimiento del diámetro máximo de la ZAF en ojos con OVR (n = 19) en comparación con el ojo contralateral (n = 19; 921 ± 213 frente a 724 ± 145 µm; p = 0,008). Además, se observó una correlación significativa entre la mejor agudeza visual corregida (MAVC) y el diámetro máximo de la ZAF en la CVP (ρ de Spearman = -0,423, p < 0,01). Por último, en los ojos con OVR, el ángulo entre el plano papilomacular y el diámetro máximo de la ZAF se dio tan solo en el 21,05% (CVS) y en el 15,79% (CVP) de los casos a 0 ± 15 ó 90 ± 15°, respectivamente. En ojos sanos, estos ángulos (que supuestamente representan una configuración de la ZAF regular) fueron más prevalentes (CVS 68,42 frente a 21,05%, p = 0,003; CVP 73,68 frente a 15,79%, p < 0,001). Conclusiones: La angio-OCT muestra alteraciones morfológicas de la ZAF en ojos con

  5. Evidence for maize (Zea mays) in the Late Archaic (3000–1800 B.C.) in the Norte Chico region of Peru

    PubMed Central

    Haas, Jonathan; Creamer, Winifred; Huamán Mesía, Luis; Goldstein, David; Reinhard, Karl; Rodríguez, Cindy Vergel

    2013-01-01

    For more than 40 y, there has been an active discussion over the presence and economic importance of maize (Zea mays) during the Late Archaic period (3000–1800 B.C.) in ancient Peru. The evidence for Late Archaic maize has been limited, leading to the interpretation that it was present but used primarily for ceremonial purposes. Archaeological testing at a number of sites in the Norte Chico region of the north central coast provides a broad range of empirical data on the production, processing, and consumption of maize. New data drawn from coprolites, pollen records, and stone tool residues, combined with 126 radiocarbon dates, demonstrate that maize was widely grown, intensively processed, and constituted a primary component of the diet throughout the period from 3000 to 1800 B.C. PMID:23440194

  6. Aryl hydrocarbon receptor-mediated activity of particulate organic matter from the Paso del Norte airshed along the U.S.-Mexico border.

    PubMed Central

    Arrieta, Daniel E; Ontiveros, Cynthia C; Li, Wen-Whai; Garcia, Jose H; Denison, Michael S; McDonald, Jacob D; Burchiel, Scott W; Washburn, Barbara Shayne

    2003-01-01

    In this study, we determined the biologic activity of dichloromethane-extracted particulate matter < 10 micro m in aerodynamic diameter (PM10) obtained from filters at three sites in the Paso del Norte airshed, which includes El Paso, Texas, USA; Juarez, Chihuahua, Mexico, and Sunland Park, New Mexico, USA. The extracts were rich in polycyclic aromatic hydrocarbons (PAHs) and had significant biologic activity, measured using two in vitro assay systems: ethoxyresorufin-(O-deethylase (EROD) induction and the aryl hydrocarbon-receptor luciferase reporter system. In most cases, both EROD (5.25 pmol/min/mg protein) and luciferase activities (994 relative light units/mg) were highest in extracts from the Advance site located in an industrial neighborhood in Juarez. These values represented 58% and 55%, respectively, of induction associated with 1 micro M ss-naphthoflavone exposures. In contrast, little activity was observed at the Northeast Clinic site in El Paso, the reference site. In most cases, luciferase and EROD activity from extracts collected from the Tillman Health Center site, situated in downtown El Paso, fell between those observed at the other two sites. Overall, a statistically significant correlation existed between PM10 and EROD and luciferase activities. Chemical analysis of extracts collected from the Advance site demonstrated that concentrations of most PAHs were higher than those reported in most other metropolitan areas in the United States. Calculations made with these data suggest a cancer risk of 5-12 cases per 100,000 people. This risk estimate, as well as comparisons with the work of other investigators, raises concern regarding the potential for adverse health effects to the residents of this airshed. Further work is needed to understand the sources, exposure, and effects of PM10 and particulate organic material in the Paso del Norte airshed. PMID:12896850

  7. Speciation and quantification of Hg in sediments contaminated by artisanal gold mining in the Gualaxo do Norte River, Minas Gerais, SE, Brazil.

    PubMed

    da Penha Rhodes, Valdilene; de Lena, Jorge Carvalho; Santolin, Camila Vidal Alves; da Silva Pinto, Thais; Mendes, Louise Aparecida; Windmöller, Cláudia Carvalhinho

    2017-12-28

    The Iron Quadrangle in SE Brazil was, in the eighteenth century, one of the most important Au producing regions of Brazil. In this region, gold is produced, even today, by artisanal methods that use Hg to increase the extraction efficiency with no control of Hg release to water systems and the atmosphere. In this context, the Gualaxo do Norte River is of particular interest; its springs are located in the Doce River basin, an important Brazilian basin that supplies water for 3.5 million people. The main goal of this work was to quantify and speciate the Hg in the sediments of the Gualaxo do Norte River using a direct mercury analyzer and gas chromatography-pyrolysis-atomic fluorescence detection system. Statistical analyses consisted of principal component analysis, aiming to assess interactions among elements and species and to group the variables in factors affecting the properties of sediment. The results show that total Hg (THg) and methylmercury (CH 3 Hg + ) concentrations in samples ranged from 209 to 1207 μg kg -1 and from 0.07 to 1.00 μg kg -1 , respectively (methylation percentages from 0.01 to 0.27%). Thermal desorption analysis showed that mercury is mainly present in the oxidized form, and correlation analyses pointed to a relationship between THg and MnO, indicating that manganese can oxidize and/or adsorb Hg. Together, MO and CH 3 Hg + are important parameters in the third principal component, indicating the influence of OM on the methylation process. This first investigation on Hg methylation in this small-scale gold mining area points to the possibility of Hg bioaccumulation and to the need of better understanding the biogeochemical cycle of Hg in this area. Samples were collected in 2012, prior to the 2015 Fundão Dam disaster. The results are also a record of the characteristics of the sediment prior to that event.

  8. Inhibitors of serine proteases decrease sperm penetration during porcine fertilization in vitro by inhibiting sperm binding to the zona pellucida and acrosome reaction.

    PubMed

    Beek, J; Nauwynck, H; Appeltant, R; Maes, D; Van Soom, A

    2015-11-01

    Serine proteases are involved in mammalian fertilization. Inhibitors of serine proteases can be applied to investigate at which point these enzymes exert their action. We selected two serine protease inhibitors, 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF, 100 μM) and soybean trypsin inhibitor (STI, 5 μM) from Glycine max, via previous dose-response IVF experiments and sperm toxicity tests. In the present study, we evaluated how these inhibitors affect porcine fertilization in vitro as calculated on total fertilization rate, polyspermy rate, and the sperm number per fertilized oocyte of cumulus-intact, cumulus-free, and zona-free oocytes. In the control group (no inhibitor), these parameters were 86%, 49%, and 2.2 for cumulus-intact oocytes and 77%, 43%, and 2.2 for cumulus-free oocytes (6-hour gamete incubation period, 1.25 × 10(5) spermatozoa/mL). 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride and STI significantly reduced total fertilization and polyspermy rate in cumulus-intact and cumulus-free oocytes (P < 0.05). Total fertilization rates were respectively 65% and 53% (AEBSF) and 36% and 17% (STI). Inhibition rates were higher in cumulus-free oocytes than in cumulus-intact oocytes, indicating that inhibitors exerted their action after sperm passage through the cumulus. 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride but not STI reduced sperm binding to the ZP. The acrosome reaction was significantly inhibited by both inhibitors. Only 40.4% (AEBSF) and 11.4% (STI) of spermatozoa completed a calcium-induced acrosome reaction compared to 86.7% of spermatozoa in the control group. There was no effect on sperm binding or fertilization parameters in zona-free oocytes. In conclusion, sperm-zona binding and acrosome reaction were inhibited by serine protease inhibitors during porcine IVF. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. MMP2 and acrosin are major proteinases associated with the inner acrosomal membrane and may cooperate in sperm penetration of the zona pellucida during fertilization.

    PubMed

    Ferrer, Marvin; Rodriguez, Hilma; Zara, Lindsay; Yu, Yang; Xu, Wei; Oko, Richard

    2012-09-01

    Sperm-zona pellucida (ZP) penetration during fertilization is a process that most likely involves enzymatic digestion of this extracellular coat by spermatozoa. Since the inner acrosomal membrane (IAM) is the leading edge of spermatozoa during penetration and proteins required for secondary binding of sperm to the zona are present on it, the IAM is the likely location of these enzymes. The objectives of this study were to identify and characterize proteinases present on the IAM, confirm their localization and provide evidence for their role in fertilization. Gelatin zymography of detergent extracts of the IAM revealed bands of enzymatic activity identified as serine and matrix metallo-proteinases (MMPs). Specific inhibitors to MMPs revealed that MMP activity was due to MMP2. Immunoblotting determined that the serine protease activity on the zymogram was due to acrosin and also confirmed the MMP2 activity. Immunogold labeling of spermatozoa at the electron microscope level showed that acrosin and MMP2 were confined to the apical and principal segments of the acrosome in association with the IAM, confirming our IAM isolation technique. Immunohistochemical examination of acrosin and MMP2 during spermiogenesis showed that both proteins originate in the acrosomic granule during the Golgi phase and later redistribute to the acrosomal membrane. Anti-MMP2 antibodies and inhibitors incorporated into in vitro fertilization media significantly decreased fertilization rates. This is the first study to demonstrate that MMP2 and acrosin are associated with the IAM and introduces the possibility of their cooperation in enzymatic digestion of the ZP during penetration.

  10. Identification of a Novel TGF-β-Binding Site in the Zona Pellucida C-terminal (ZP-C) Domain of TGF-β-Receptor-3 (TGFR-3)

    PubMed Central

    Diestel, Uschi; Resch, Marcus; Meinhardt, Kathrin; Weiler, Sigrid; Hellmann, Tina V.; Mueller, Thomas D.; Nickel, Joachim; Eichler, Jutta; Muller, Yves A.

    2013-01-01

    The zona pellucida (ZP) domain is present in extracellular proteins such as the zona pellucida proteins and tectorins and participates in the formation of polymeric protein networks. However, the ZP domain also occurs in the cytokine signaling co-receptor transforming growth factor β (TGF-β) receptor type 3 (TGFR-3, also known as betaglycan) where it contributes to cytokine ligand recognition. Currently it is unclear how the ZP domain architecture enables this dual functionality. Here, we identify a novel major TGF-β-binding site in the FG loop of the C-terminal subdomain of the murine TGFR-3 ZP domain (ZP-C) using protein crystallography, limited proteolysis experiments, surface plasmon resonance measurements and synthetic peptides. In the murine 2.7 Å crystal structure that we are presenting here, the FG-loop is disordered, however, well-ordered in a recently reported homologous rat ZP-C structure. Surprisingly, the adjacent external hydrophobic patch (EHP) segment is registered differently in the rat and murine structures suggesting that this segment only loosely associates with the remaining ZP-C fold. Such a flexible and temporarily-modulated association of the EHP segment with the ZP domain has been proposed to control the polymerization of ZP domain-containing proteins. Our findings suggest that this flexibility also extends to the ZP domain of TGFR-3 and might facilitate co-receptor ligand interaction and presentation via the adjacent FG-loop. This hints that a similar C-terminal region of the ZP domain architecture possibly regulates both the polymerization of extracellular matrix proteins and cytokine ligand recognition of TGFR-3. PMID:23826237

  11. NEFM (Neurofilament Medium) Polypeptide, a Marker for Zona Glomerulosa Cells in Human Adrenal, Inhibits D1R (Dopamine D1 Receptor)-Mediated Secretion of Aldosterone.

    PubMed

    Maniero, Carmela; Garg, Sumedha; Zhao, Wanfeng; Johnson, Timothy Isaac; Zhou, Junhua; Gurnell, Mark; Brown, Morris J

    2017-08-01

    Heterogeneity among aldosterone-producing adenomas (APAs) has been highlighted by the discovery of somatic mutations. KCNJ5 mutations predominate in large zona fasciculata (ZF)-like APAs; mutations in CACNA1D , ATP1A1, ATP2B3 , and CTNNB1 are more likely to be found in small zona glomerulosa (ZG)-like APAs. Microarray comparison of KCNJ5 mutant versus wild-type APAs revealed significant differences in transcriptomes. NEFM , encoding a neurofilament subunit which is a D1R (dopamine D1 receptor)-interacting protein, was 4-fold upregulated in ZG-like versus ZF-like APAs and 14-fold more highly expressed in normal ZG versus ZF. Immunohistochemistry confirmed selective expression of NEFM (neurofilament medium) polypeptide in ZG and in ZG-like APAs. Silencing NEFM in adrenocortical H295R cells increased basal aldosterone secretion and cell proliferation; silencing also amplified aldosterone stimulation by the D1R agonist, fenoldopam, and inhibition by the D1R antagonist, SCH23390. NEFM coimmunoprecipitated with D1R, and its expression was stimulated by fenoldopam. Immunohistochemistry for D1R was mainly intracellular in ZG-like APAs but membranous in ZF-like APAs. Aldosterone secretion in response to fenoldopam in primary cells from ZF-like APAs was higher than in cells from ZG-like APAs. Transfection of mutant KCNJ5 caused a large reduction in NEFM expression in H295R cells. We conclude that NEFM is a negative regulator of aldosterone production and cell proliferation, in part by facilitating D1R internalization from the plasma membrane. Downregulation of NEFM in ZF-like APAs may contribute to a D1R/D2R imbalance underlying variable pharmacological responses to dopaminergic drugs among patients with APAs. Finally, taken together, our data point to the possibility that ZF-like APAs are in fact ZG in origin. © 2017 American Heart Association, Inc.

  12. Diversity, origin and tectonic significance of the Mesoarchean granitoids of Ourilândia do Norte, Carajás province (Brazil)

    NASA Astrophysics Data System (ADS)

    da Silva, Luciano Ribeiro; de Oliveira, Davis Carvalho; dos Santos, Maria Nattânia Sampaio

    2018-03-01

    This study investigates the diversity, origin and tectonic significance of the Ourilândia do Norte Mesoarchean granitoids, emplaced near Rio Maria-Carajás domains boundary, southeastern Amazonian Craton (Brazil). In this area, previous works has identified sanukitoids (∼2.87 Ga), (quartz) diorites of BADR affinity and undifferentiated leucogranites, with charnockites cross-cutting the other granitoids. New geological mapping data allowed to differentiate three new groups of granitoids: (i) biotite monzogranites (BMzG); (ii) epidote-biotite granodiorites (EBGd); and (iii) porphyritic granitoids (pGrt). Thus, this paper aims to define their classification, nature, formation processes and deformation aspects, and discuss the relations between plutonism and deformation for the Ourilandia do Norte granitoids. The petrographic data showed that each one of these groups can be subdivided into two facies. The BMzG is differentiated into equigranular (eBMzG) and heterogranular (hBMzG) and the EBGd into heterogranular (hEBGd) and sparsely porphyritic (spTEBGd). These granitoids constitute two batholiths separated by a rock strip of sanukitoid and BADR affinities. Both are largely dominated by BMzG rocks, with less abundant EBGd lenses. The pGrt is individualized in porphyritic granodiorites (pBHGd) and trondhjemites (pEBTd), which occur as smaller bodies. Structurally, the central portions these plutons represent lower strain domains, while their borders are marked by large-scale shear zones, where occur submagmatic and mylonitic fabrics of ENE-WSW main direction and subvertical dip, respectively. The meso- and microstructural data indicate that the rocks studied are syn-to late-tectonic and were affected by high temperature deformation (>500 °C) and low differential stress, in a sinistral transpression regime, indicating that both fabrics are related to the a same deformational event. Geochemically, except the pEBTd facies that has sodic affinity, the Ourilândia do

  13. [Prevalence of hepatitis C virus in the blood bank at Centro Médico Nacional La Raza].

    PubMed

    Benítez-Arvizu, Gamaliel; Cortez-Gómez, Rudyard; Novelo-Garza, Bárbara Alicia; Malagón-Martínez, Araceli; Guerra-Márquez, Angel; Alvarado-Maldonado, María Del Consuelo; Rodríguez-Bartolo, Mireya; Argüelles-Pimentel, Rosa María; Sánchez-Barrera, Reyna Gabriela

    2006-01-01

    There are no records on the prevalence of infection by HCV in Mexican population. The central area of Mexico is a highly dense demographic zone and is the influence area of the second blood bank in Latin America in terms of affluence. We prospectively studied the prevalence and genotypes of HCV infection in 5105 individuals attending the Central Blood Bank of Centro Médico Nacional La Raza regardless if they were accepted or rejected as donors. We applied a quimiolumiscence assay as a screening test. A recombinant immunoassay (RIBA) and a qualitative polymerase chain reaction (PCR) were performed as confirmatory tests and to detect viremia, respectively. Virus genotype was identified by means of a Line Immuno Probe Assay in PCR positive samples. The overall prevalence of HCV infection was 0.195% (10/5105). Viremia was detected in 90% of the subjects. The prevalence of accepted donors (0.087%) was significantly lower (p = 0.017) than that of the rejected ones (0.421%). Among viremic subjects, 60 % were infected with genotype 2 and 40% with a subtype combination (a/b) of genotype 1. The prevalence of HCV infection in our population was significantly lower than the world mean prevalence estimated in 3 %. A higher prevalence of genotype 2 in asymptomatic individuals contrasts with previous studies with a selected population where genotype 1 prevailed.

  14. Three-dimensional geologic modeling to determine the spatial attributes of hydrocarbon contamination, Noval Facility Fuel Farm, El Centro, California

    SciTech Connect

    Johnson, C.; Mutch, S.; Padgett, D.

    An investigation was conducted at the Naval Air Facility located in El Centro (NAFEC), to determine the vertical and horizontal extent of hydrocarbon contamination at the facilities fuel farm. The fuel products are the result of tank and pipeline leakage, past tank cleaning, and past disposal of fuel dispensing and filter cleaning practices. Subsurface soil and groundwater data was collected via soil borings, monitoring wells, and CPT probes. Soil, groundwater, and analytical data were integrated using the LYNX geoscience modeling system (GMS). Interactive sessions with the data visualizer helped guide the modeling and identify data gaps. Modeling results indicate amore » continuous surface confining clay layer to a depth of about 12 to 15 ft. Groundwater is confined beneath this clay layer and monitoring wells indicate about 3 to 5 ft of artesian head. Hydrocarbon contamination is concentrated within this clay layer from about 5 to 12 ft below the ground surface. Residual fuel products located in the groundwater are attributed to slow leakage through the confirming clay layer. LYNX was also used to compute volumes of contaminated soil to aid in remediation cost analysis. Preliminary figures indicate about 60,000 yards[sup 3] of contaminated soil. Since the contamination is primarily confined to relatively impermeable clayey soils, site remediation will likely be ex-situ land farming.« less

  15. Benign childhood epilepsy with centro-temporal spikes: quantitative EEG and the Wechsler intelligence scale for children (WISC-III).

    PubMed

    Tedrus, Gloria M A S; Fonseca, Lineu C; Tonelotto, Josiane M F; Costa, Rebeca M; Chiodi, Marcelo G

    2006-07-01

    Benign childhood epilepsy with centro-temporal spikes (BECTS) is a form of focal idiopathic epilepsy, with seizure remission by the age of 18. Recent studies have suggested that some children with BECTS can suffer from deficits of memory, attention and learning ability and in auditory-verbal and performance sub-tests. On the other hand, alterations in the baseline brain electrical activity determined by using the quantitative electroencephalogram (qEEG) have been described. The objective of this study was to evaluate the absolute and relative powers in the delta, theta, alpha and beta bands of the qEEG in children with BECTS, and their relation to IQ measurements (WISC-III). Twenty-six 8 to 11-year-old children with BECTS were studied, paired with a control group of healthy children according to age and gender. It was shown that the absolute delta and theta powers were statistically greater in the children with BECTS than in the control group, at almost all the electrodes. In the children with BECTS, a negative correlation (Pearson's correlation test) was observed at various electrodes between the absolute delta and theta powers and the performance IQ. These data indicate a possible relationship between maturational disturbance in the brain electrical activity development and the tendency for inferior cognitive performance in children with BECTS.

  16. Epidemiologic Profile of Patients Transplanted With Hematopoietic Stem Cells in a Reference Service in the State of Rio Grande do Norte, Brazil.

    PubMed

    Campos de Azevedo, I; Ferreira Júnior, M A; Pereira de Aquino, L A; de Oliveira, A A; Cruz, G K P; de Queiroz Cardoso, A I; Ivo, M L; Santos, V E P

    2018-04-01

    Hematopoietic stem cell transplantation (HSCT) consists of the intravenous infusion of healthy hematopoietic stem cells to restore the medullary and immunologic function of patients affected by a series of hematologic, oncologic, immunologic, malignant and nonmalignant inherited or acquired diseases, with the possibility of cure or increase of disease-free survival. To characterize the epidemiologic profile and the cases of death of patients who underwent HSCT. This is a cohort quantitative study, nested with a retrospective, descriptive, and analytical study of a hospital-based cohort that included the patients who underwent HSCT at a referral service in the state of Rio Grande do Norte, a region of northeastern Brazil. There was a slight male prevalence (52.94%), the age of the patients ranged from 2 to 73 years old, 18.38% were brown, 47.06% were married, 15.07% were students, 78.31% had a diagnosis of multiple myeloma, 93.38% developed gastrointestinal toxicities, all patients received chemotherapeutic treatment, 54.78% had allogeneic HSCT, and the cause of the most recorded deaths was septic shock (48.19%). This study showed relevant scientific evidence on the clinical and epidemiologic profile of patients who underwent HSCT. In general, sociodemographic data are similar to national and international research results. Copyright © 2018 Elsevier Inc. All rights reserved.

  17. Modified team-based learning strategy to improve human anatomy learning: A pilot study at the Universidad del Norte in Barranquilla, Colombia.

    PubMed

    Martínez, Emilio G; Tuesca, Rafael

    2014-01-01

    As part of an institutional program sponsored by the Centre for Teaching Excellence at the Universidad del Norte, Barranquilla, Colombia, we developed an educational research study on two sessions of human anatomy in which we combined team-based learning (TBL) and the use of iPads. Study data included the TBL, assessments applied during the course, student's grades on mid-term examinations and students' perceptions of their experiences. Students reported a positive attitude toward the use of the TBL sessions, and the results showed a significant improvement in their learning between the first and second sessions. Significantly positive correlations (P < 0.05) were obtained between (a) the individual students' readiness test performance 1 and mid-term examination 1, (b) the individual readiness test performances from Session 1 to Session 2, and (c) the group readiness test performances from the first and second sessions. These results point to positive learning experiences for these students. Analyses of the students' reflections on their activities also pointed toward future challenges. © 2014 American Association of Anatomists.

  18. Vigilando la Calidad del Agua de los Grandes Rios de la Nacion: El Programa NASQAN del Rio Grande (Rio Bravo del Norte)

    USGS Publications Warehouse

    Lurry, Dee L.; Reutter, David C.; Wells, Frank C.; Rivera, M.C.; Munoz, A.

    1998-01-01

    La Oficina del Estudio Geologico de los Estados Unidos (U.S. Geological Survey, 0 USGS) ha monitoreado la calidad del agua de la cuenca del Rio Grande (Rio Bravo del Norte) desde 1995 como parte de la rediseiiada Red Nacional para Contabilizar la Calidad del Agua de los Rios (National Stream Quality Accounting Network, o NASOAN) (Hooper and others, 1997). EI programa NASOAN fue diseiiado para caracterizar las concentraciones y el transporte de sedimento y constituyentes quimicos seleccionados, encontrados en los grandes rios de los Estados Unidos - incluyendo el Misisipi, el Colorado y el Columbia, ademas del Rio Grande. En estas cuatro cuencas, el USGS opera actualmente (1998) una red de 40 puntos de muestreo pertenecientes a NASOAN, con un enfasis en cuantificar el flujo en masa (la cantidad de material que pasa por la estacion, expresado en toneladas por dial para cada constituyente. Aplicacando un enfoque consistente, basado en la cuantificacion de flujos en la cuenca del Rio Grande, el programa NASOAN esta generando la informacion necesaria para identificar fuentes regionales de diversos contaminantes, incluyendo sustancias qui micas agricolas y trazas elementos en la cuenca. EI efecto de las grandes reservas en el Rio Grande se puede observar segun los flujos de constituyentes discurren a 10 largo del rio. EI analisis de los flujos de constituyentes a escala de la cuenca proveera los medios para evaluar la influencia de la actividad humana sobre las condiciones de calidad del agua del Rio Grande.

  19. fMRI brain response during sentence reading comprehension in children with benign epilepsy with centro-temporal spikes.

    PubMed

    Malfait, D; Tucholka, A; Mendizabal, S; Tremblay, J; Poulin, C; Oskoui, M; Srour, M; Carmant, L; Major, P; Lippé, S

    2015-11-01

    Children with benign epilepsy with centro-temporal spikes (BECTS) often have language problems. Abnormal epileptic activity is found in central and temporal brain regions, which are involved in reading and semantic and syntactic comprehension. Using functional magnetic resonance imaging (fMRI), we examined reading networks in BECTS children with a new sentence reading comprehension task involving semantic and syntactic processing. Fifteen children with BECTS (age=11y 1m ± 16 m; 12 boys) and 18 healthy controls (age=11 y 8m ± 20 m; 11 boys) performed an fMRI reading comprehension task in which they read a pair of syntactically complex sentences and decided whether the target sentence (the second sentence in the pair) was true or false with respect to the first sentence. All children also underwent an exhaustive neuropsychological assessment. We demonstrated weaknesses in several cognitive domains in BECTS children. During the sentence reading fMRI task, left inferior frontal regions and bilateral temporal areas were activated in BECTS children and healthy controls. However, additional brain regions such as the left hippocampus and precuneus were activated in BECTS children. Moreover, specific activation was found in the left caudate and putamen in BECTS children but not in healthy controls. Cognitive results and accuracy during the fMRI task were associated with specific brain activation patterns. BECTS children recruited a wider network to perform the fMRI sentence reading comprehension task, with specific activation in the left dorsal striatum. BECTS cognitive performance differently predicted functional activation in frontal and temporal regions compared to controls, suggesting differences in brain network organisation that contribute to reading comprehension. Crown Copyright © 2015. Published by Elsevier B.V. All rights reserved.

  20. Study on the zona pellucida 4 (ZP4) gene sequence and its expression in the ovaries of patients with polycystic ovary syndrome.

    PubMed

    Meczekalski, B; Nawrot, R; Nowak, W; Czyzyk, A; Kedzia, H; Gozdzicka-Jozefiak, A

    2015-07-01

    Polycystic ovary syndrome (PCOS) is a common endocrine disorder of unknown pathology, involving reproductive and metabolic abnormalities. Oocyte-specific genes are a group of genes expressed exclusively in ovarian tissue; therefore, they can play an important role in ovarian pathologies such as PCOS. The zona pellucida 4 (ZP4) gene encodes glycoprotein which is a part of the extracellular matrix of oocyte. We analyzed 87 patients with PCOS, which were divided into four groups depending on their phenotype. In each patient, we performed profound clinical and biochemical analysis, including the measurement of serum androgens. The ovarian tissue samples were used to perform a real-time polymerase chain reaction and immunohistochemical staining using anti-ZP4 monoclonal antibodies. The ZP4 gene was sequenced from peripheral lymphocytes. The expression of ZP4 was present in early antral follicles and was stronger in mature follicles. The subgroup of patients with eumenorrhea and without hyperandrogenism presented the highest expression of ZP4 in ovarian tissue. In one case, we found a mutation of the ZP4 gene. No correlations were found between the ZP4 expression level and biochemical or clinical indices. Data from this and animal studies suggest a possible relationship between androgens and ZP4 expression. ZP4 expression is highest among patients with PCOS and a regular cycle, and this is a consequence of the presence of mature follicles in this group. In some patients with PCOS and infertility, ZP4 mutation can be found.

  1. In-vitro developmental potential of individual mouse blastomeres cultured with and without zona pellucida: future implications for human assisted reproduction.

    PubMed

    Illmensee, K; Kaskar, K; Zavos, P M

    2006-08-01

    This study was designed to compare the developmental potential of individual blastomeres derived from 2-, 4-, 6- and 8-cell mouse embryos cultured with and without zona pellucida (ZP). In the first series, one, three, five and seven blastomeres were biopsied from 2-, 4-, 6- and 8-cell embryos respectively, and inserted individually into empty ZP recipients, leaving the remaining blastomere within its original ZP. In the second series, the same protocol was used except that the biopsied blastomeres were cultured without ZP and compared with the remaining blastomere within its original ZP. For the first series, individual blastomeres derived from 2-, 4-, 6- and 8-cell embryos cultured with ZP showed blastocyst development of 82.4, 68.6, 44.4 and 23.1% respectively, with corresponding hatching rates of 70.6, 60.0, 25.9 and 7.7%. For the second series, individual blastomeres cultured without ZP progressed with blastocyst development of 73.3, 64.5, 35.7 and 22.7% respectively. Blastocyst multiplication was achieved most efficiently when using individual blastomeres from 4- and 6-cell embryos. This is the first report on comparative in-vitro propagation of single blastomeres derived from various cleavage stages in a mammalian species. Blastomere cloning with its multiple applications may be envisaged for human assisted reproductive technologies.

  2. [Sexuality and STD/AIDS prevention: social representations by rural men in a county in the Zona da Mata region in Pernambuco, Brazil].

    PubMed

    Alves, Maria de Fátima Paz

    2003-01-01

    This study analyzes the concepts displayed by rural men in the Zona da Mata region in the State of Pernambuco, Brazil, concerning their sexual practices and STD/AIDS prevention. The study adopts a qualitative methodology, having interviewed 22 men According to the interviews, their first sexual intercourse is characterized as a learning experience and is sometimes marked by violence. They make a distinction between the "woman at home" and "street women"; they acknowledge women's sexual desire and value reciprocity in sexual relations, differentiating between the kinds of sex they have with different categories of women. Seven men report homoerotic experiences during adolescence, which they ascribe to immaturity, not affecting their heterosexual identity. Condom use, perceived in a negative light, is inconstant and irregular, inversely proportional to knowing the female partner. STDs in general inspire little fear, while AIDS is associated with death; the interviewees do not see themselves at risk of acquiring HIV. Ambiguities in the men's discourse, together with a basically ineffective approach by health services and preventive campaigns, reveal a high level of exposure to the risk of contracting STDs/AIDS among the interviewees and their female or male partners.

  3. Roles of the zona pellucida and functional exposure of the sperm-egg fusion factor 'IZUMO' during in vitro fertilization in pigs.

    PubMed

    Tanihara, Fuminori; Nakai, Michiko; Men, Nguyen Thi; Kato, Noriko; Kaneko, Hiroyuki; Noguchi, Junko; Otoi, Takeshige; Kikuchi, Kazuhiro

    2014-04-01

    The zona pellucida (ZP) is considered to play important roles in the prevention of polyspermy in mammalian oocytes. However, in pigs we have shown that the presence of the ZP accelerates sperm penetration into the ooplasm during in vitro fertilization (IVF). In the present study, we investigated the effects of the ZP on sperm binding, acrosomal status, and functional exposure of IZUMO, a critical factor involved in sperm-egg fusion, during IVF in pigs. We evaluated the numbers and acrosomal statuses of sperm binding to the ZP and oolemma, and being present in the ZP and perivitelline space (PVS) using ZP-intact and ZP-free oocytes. More sperm bound to the ZP than to the oolemma. The average number of sperm present in the PVS was 0.44-0.51 per oocyte, and all sperm had lost their acrosomes. The proportion of sperm that were immunopositive for anti-IZUMO antibody was significantly higher after they were passing or had passed through the ZP. Furthermore, addition of anti-IZUMO antibody to the fertilization medium significantly inhibited the penetration of sperm into ZP-free oocytes. These results suggest that, in pigs, the ZP induces the acrosome reaction, which is associated with the functional exposure of IZUMO, resulting in completion of fertilization. © 2014 Japanese Society of Animal Science.

  4. Production of tag-free recombinant fusion protein encompassing promiscuous T cell epitope of tetanus toxoid and dog zona pellucida glycoprotein-3 for contraceptive vaccine development.

    PubMed

    Gupta, Neha; Shrestha, Abhinav; Panda, Amulya Kumar; Gupta, Satish Kumar

    2013-07-01

    Affinity tags can interfere in various physicochemical properties and immunogenicity of the recombinant proteins. In the present study, tag-free recombinant fusion protein encompassing promiscuous T cell epitope of tetanus toxoid [TT; amino acid (aa) residues 830-844] followed by dilysine linker and dog zona pellucida glycoprotein-3 (ZP3; aa residues 23-348) (TT-KK-ZP3) was expressed in Escherichia coli. The recombinant protein, expressed as inclusion bodies (IBs), was purified by isolation of IBs, processed to remove host cell proteins, followed by solubilization and refolding. A specific 39 kDa protein including ZP3 was identified by SDS-PAGE. CD spectra showed the presence of α-helices and β-sheets, and fluorescent spectroscopy revealed emission maxima of 265 A.U. at 339 nm for refolded protein and showed red shift in the presence of 6 M guanidine hydrochloride. Immunization of inbred FvB/J female mice with purified recombinant TT-KK-ZP3 (25 μg/animal) led to generation of high antibody titers against the recombinant protein. The antibodies reacted specifically with ZP matrix surrounding mouse oocytes. Immunized mice showed significant reduction in fertility as compared to the control group. The studies described herein provide a simple method to produce and purify tag-free recombinant protein for the development of a contraceptive vaccine.

  5. Caudal Zona Incerta/VOP Radiofrequency Lesioning Guided by Combined Stereotactic MRI and Microelectrode Recording for Posttraumatic Midbrain Resting-Kinetic Tremor.

    PubMed

    Contreras Lopez, William Omar; Azevedo, Angelo R; Cury, Rubens G; Alencar, Francisco; Neville, Iuri S; Reis, Paul R; Navarro, Jessie; Monaco, Bernardo; da Silva, Fabio E Fernandes; Teixeira, Manoel J; Fonoff, Erich T

    2016-02-01

    Reporting the outcome of two patients who underwent unilateral ablative stereotactic surgery to treat pharmacologic resistant posttraumatic tremor (PTT). We present two patients (31 and 47 years old) with refractory PTT severely affecting their quality of life. Under stereotactic guidance, refined by T2-weighted magnetic resonance imaging and double-channel multiunit microelectrode recording (MER), three sequential radiofrequency lesions were performed in the caudal zona incerta (cZi) up to the base of thalamus (VOP). Effects of cZi/VOP lesion were prospectively rated with a tremor rating scale. Both patients demonstrated intraoperative tremor suppression with sustained results up to 18 months follow-up, with improvement of 92% and 84%, respectively, on the tremor rating scale. Tremor improvement was associated with enhancement functionality and quality of life for the patients. The patients returned to their work after the procedure. No adverse effects were observed up to the last follow-up. Radiofrequency lesion of the cZi/VOP target was effective for posttraumatic tremor in both cases. The use of T2-weighted images and MER was found helpful in increasing the precision and safety of the procedure, because it leads the RF probe by relying on neighbor structures based on thalamus and subthalamic nucleus. Copyright © 2016 Elsevier Inc. All rights reserved.

  6. The Morphologic Evolution of the Amazon Coastal Plain, Cabo Norte, Amapa, Brazil: The Need for Integrated Investigation on the Internal Continental Shelf.

    NASA Astrophysics Data System (ADS)

    Silveira, O.; Santos, V. F.; Takiyama, L. R.

    2007-05-01

    refers to the coastal zone between the Amapa Grande River and Araguari River, including Maraca Island and the Oriental lacustrine Belt. Westward the island, at least three paleolevels of clays with roots in life position suggests regressive/transgressive events. Extraordinaty paleodrainage network beginnig at the continent and recognized at the insular portion suggests links with the paleochannels found at the continental shelf. The Oriental Belt of lakes is located close to the coastline, at Cabo Norte. It main feature is a mud lump approximately 10 Km ratio, well recognized at the remote sensing. It shows similar evolutionary processes with Araguari River, dating from XIX century, when this river had two mouths defined by the Carpori Island. The reasons of the deactivation are still unknowed, but, this rapid morphological evolution indicates short time colmatation processes that can be linked to tectonic regional processes. On the other hand, the Cabo Norte feature consolidation may impose changes in the sedimentation processes yielding space reduction over the coastal plain accumulation, diminishing of the solid and liquid fluvial discharge and promoting the availability of the local sediment transport over the littoral. The investigation of these processes requires an integrated coastal plain-continental shelf morphological study applying adequate techniques for modification studies and dating ages over short geological time frame, in century scale level.

  7. Integrative research identifies 71 new plant species records in the state of Rio Grande do Norte (Brazil) and enhances a small herbarium collection during a funding shortage

    PubMed Central

    Versieux, Leonardo M.; Dávila, Nállarett; Delgado, Geadelande C.; de Sousa, Valdeci F.; de Moura, Edweslley Otaviano; Filgueiras, Tarciso; Alves, Marccus V.; Carvalho, Eric; Piotto, Daniel; Forzza, Rafaela C.; Calvente, Alice; Jardim, Jomar G.

    2017-01-01

    Abstract A National Forest Inventory (NFI) encompassing the entire territory of Brazil is in progress. It is coordinated and promoted by the Brazilian Forest Service of the Ministry of Environment. In each state, the NFI collaborates with local herbaria by receiving collected plant material and performing species identification. Consultants are hired by the NFI and work at the local herbaria under the supervision of a curator. In exchange for curatorial assistance, the NFI provides equipment and consumables for the herbarium. Other public projects collaborating with NFI are Reflora and the Brazilian Biodiversity Information System (SiBBr). Both projects have online platforms that seek to connect herbaria and make all their data freely available, including high quality digital images of specimens. Through inter-institutional collaboration, the joint interests of NFI, Reflora, SiBBr and local herbaria have improved collections, expanded the online Reflora database, and provided the NFI with verified species lists. These strategic uses of public funding are positively affecting Botany, particularly during a period of economic crisis and cuts in research. Here, we illustrate the increase in floristic knowledge through the improvement of a herbarium collection in Rio Grande do Norte (RN) – the Brazilian state with the lowest levels of plant richness. We report 71 new occurrences of vascular plants for RN, belonging mainly to the Poaceae, Fabaceae and Malvaceae. Most of the species with new occurrences have a Neotropical distribution (21 spp.) and only seven are restricted to the Brazilian Northeast. Our findings highlight previous gaps in RN’s floristic knowledge. The partnership NFI, Reflora, SiBBr and the UFRN herbarium improved herbarium curation, digital collection, and quality of data. Finally, a fellowship provided by Reflora and SiBBr allowed improving curation by distributing duplicates and incorporating the Herbarium of Câmara Cascudo Museum. PMID:29033668

  8. Mapping of High Value Crops Through AN Object-Based Svm Model Using LIDAR Data and Orthophoto in Agusan del Norte Philippines

    NASA Astrophysics Data System (ADS)

    Candare, Rudolph Joshua; Japitana, Michelle; Cubillas, James Earl; Ramirez, Cherry Bryan

    2016-06-01

    This research describes the methods involved in the mapping of different high value crops in Agusan del Norte Philippines using LiDAR. This project is part of the Phil-LiDAR 2 Program which aims to conduct a nationwide resource assessment using LiDAR. Because of the high resolution data involved, the methodology described here utilizes object-based image analysis and the use of optimal features from LiDAR data and Orthophoto. Object-based classification was primarily done by developing rule-sets in eCognition. Several features from the LiDAR data and Orthophotos were used in the development of rule-sets for classification. Generally, classes of objects can't be separated by simple thresholds from different features making it difficult to develop a rule-set. To resolve this problem, the image-objects were subjected to Support Vector Machine learning. SVMs have gained popularity because of their ability to generalize well given a limited number of training samples. However, SVMs also suffer from parameter assignment issues that can significantly affect the classification results. More specifically, the regularization parameter C in linear SVM has to be optimized through cross validation to increase the overall accuracy. After performing the segmentation in eCognition, the optimization procedure as well as the extraction of the equations of the hyper-planes was done in Matlab. The learned hyper-planes separating one class from another in the multi-dimensional feature-space can be thought of as super-features which were then used in developing the classifier rule set in eCognition. In this study, we report an overall classification accuracy of greater than 90% in different areas.

  9. An analysis of the meanings of pre-eclampsia for pregnant and postpartum women and health professionals in Rio Grande do Norte, Brazil.

    PubMed

    de Azevedo, Daniela Vasconcelos; de Araújo, Ana C P F; Clara Costa, Iris C

    2011-12-01

    To understand the meanings of pre-eclampsia for pregnant and postpartum women and health-care professionals. a word-association test and semi-structured interviews. A maternity hospital located in Natal, Rio Grande do Norte, Brazil that serves pregnant and postpartum women considered to be at high risk. 51 Pregnant women, 10 postpartum women and 87 health-care professionals completed a word-association test; 18 pregnant women, two postpartum women and 20 health-care professionals for the interviews. Thematic categories based on the word-association test and the interviews were created to help the data analysis. χ(2) test was used to compare the categories raised by both groups in the word-association test to determine the association between the frequencies of these categories. The meanings of pre-eclampsia to pregnant and postpartum women were fear, risk, care and lack of information. To the health-care professionals, the meanings were care, fear, risk, high blood pressure, oedema and proteinuria. The frequencies of the categories 'fear', 'care' and 'risk' were statistically different (χ(2) = 31.84, 14.5, 38.19, respectively; df = 2, p < 0.001) between the group of pregnant and postpartum women and the group of health-care professionals. For the first group, the most significant meanings were fear and risk, compared with care for the second group. The analysis of the interviews confirmed and deepened the results of the word-association test, and also demonstrated that the pregnant and postpartum women had no information about pre-eclampsia. There is a gap between how women and health professionals view their experiences of pre-eclampsia. A warm welcome to the health-care facility, with clear explanations about the disease and the procedures to be performed during the visits and hospitalisation would be important aspects for the physical and mental well-being of pregnant and postpartum women. Copyright © 2010 Elsevier Ltd. All rights reserved.

  10. Integrative research identifies 71 new plant species records in the state of Rio Grande do Norte (Brazil) and enhances a small herbarium collection during a funding shortage.

    PubMed

    Versieux, Leonardo M; Dávila, Nállarett; Delgado, Geadelande C; de Sousa, Valdeci F; de Moura, Edweslley Otaviano; Filgueiras, Tarciso; Alves, Marccus V; Carvalho, Eric; Piotto, Daniel; Forzza, Rafaela C; Calvente, Alice; Jardim, Jomar G

    2017-01-01

    A National Forest Inventory (NFI) encompassing the entire territory of Brazil is in progress. It is coordinated and promoted by the Brazilian Forest Service of the Ministry of Environment. In each state, the NFI collaborates with local herbaria by receiving collected plant material and performing species identification. Consultants are hired by the NFI and work at the local herbaria under the supervision of a curator. In exchange for curatorial assistance, the NFI provides equipment and consumables for the herbarium. Other public projects collaborating with NFI are Reflora and the Brazilian Biodiversity Information System (SiBBr). Both projects have online platforms that seek to connect herbaria and make all their data freely available, including high quality digital images of specimens. Through inter-institutional collaboration, the joint interests of NFI, Reflora, SiBBr and local herbaria have improved collections, expanded the online Reflora database, and provided the NFI with verified species lists. These strategic uses of public funding are positively affecting Botany, particularly during a period of economic crisis and cuts in research. Here, we illustrate the increase in floristic knowledge through the improvement of a herbarium collection in Rio Grande do Norte (RN) - the Brazilian state with the lowest levels of plant richness. We report 71 new occurrences of vascular plants for RN, belonging mainly to the Poaceae, Fabaceae and Malvaceae. Most of the species with new occurrences have a Neotropical distribution (21 spp.) and only seven are restricted to the Brazilian Northeast. Our findings highlight previous gaps in RN's floristic knowledge. The partnership NFI, Reflora, SiBBr and the UFRN herbarium improved herbarium curation, digital collection, and quality of data. Finally, a fellowship provided by Reflora and SiBBr allowed improving curation by distributing duplicates and incorporating the Herbarium of Câmara Cascudo Museum.

  11. Survey infravermelho do braço espiral de scuttum e da barra no centro galáctico

    NASA Astrophysics Data System (ADS)

    Amôres, E. B.; Lépine, J. R. D.

    2003-08-01

    O survey infravermelho consiste de observações utilizando a CAMIV no telescópio de 60 cm do LNA. Nosso principal objetivo é melhor caracterizar a população estelar dos braços espirais e de uma estrutura existente no centro galáctico que acreditamos ser devido à existência de uma barra, cujas extremidades estariam localizadas em longitudes galácticas l~23° e l~14°. As observações realizadas em vários filtros (imagens J, H e K) são particularmente importantes nas direções que tangenciam os braços espirais, desta forma, comparamos uma direção que atravessa um braço a uma direção próxima que não passa pelo braço, sendo que ambas possuem a mesma contribuição de contagens foreground. Nas missões realizadas até o presente, observamos a região na direção tangencial ao braço de Scuttum, com observações de l = 30.04° até l = 31.04°, a cada intervalo de longitude de 0.1°, para latitude galáctica de 0.0°, 0.25°, 0.50°. Para as regiões que acreditamos serem as extremidades da barra, observamos as faixas de longitude compreendidas entre -15° £ l £ -13° e 24° £ l £ 22° a cada intervalo de 0.25°, para latitude galáctica de 0.0° e 0.25°. As observações nos filtros J e H foram feitas com tempo de integração de 60s, na banda K, as observações foram feitas utilizando o filtro C1 com tempo de integração de aproximadamente 210 s (12x15s). Elaboramos diagramas cor-cor e cor-magnitude onde podemos verificar a existência de regiões com diferentes contagens e população estelar à medida que um braço espiral é atravessado.

  12. Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen.

    PubMed

    Liu, De Yi; Liu, Ming Li; Garrett, Claire; Baker, H W Gordon

    2007-07-01

    The aim of this study was to compare the frequency of defective sperm-zona pellucida (ZP) binding (DSZPB) and defective ZP-induced acrosome reaction (DZPIAR) in subfertile men (i.e. male partners of infertile couples) with normal and abnormal semen analyses. A total of 1030 subfertile men with normal semen analysis (n=255), oligozoospermia (count<20x10(6)/ml, n=136), severe teratozoospermia (strict normal morphology

  13. Evaluation of tight junction protein 1 encoding zona occludens 1 as a candidate gene for albuminuria in a Mexican American population.

    PubMed

    Lehman, D M; Leach, R J; Johnson-Pais, T; Hamlington, J; Fowler, S; Almasy, L; Duggirala, R; Stern, M P; Abboud, H E

    2006-09-01

    Albuminuria, a hallmark of diabetic nephropathy, has been shown to be significantly heritable in multiple studies. Therefore, the identification of genes that affect susceptibility to albuminuria may lead to novel avenues of intervention. Current evidence suggests that the podocyte and slit diaphragm play a key role in controlling the selective sieve of the glomerular filtration barrier, and podocyte-specific genes have been identified that are necessary for maintaining its integrity. We therefore investigated the role of gene variants of tight junction protein (TJP1) which encodes another slit diaphragm-associated protein zona occludens 1 as risk factors for albuminuria in the San Antonio Family Diabetes/Gallbladder Study (SAFDGS), which consists of extended Mexican-American families with a high prevalence of type 2 diabetes. Albuminuria, defined as an albumin (mg/dl) to creatinine (mg/dl) ratio (ACR) of 0.03, which is approximately equivalent to a urinary albumin excretion (UAE) >30 mg/day, was present in a total of 14.9% of participants, and 31% had type 2 diabetes. The TJP1 exons, flanking intronic sequence, and putative proximal promoter regions were investigated in this population. Twentynine polymorphisms, including 7 nonsynonymous SNPs, were identified and genotyped in all subjects of this study for association analysis. Three sets of correlated SNPs, which include 3 exonic SNPs, were nominally associated with ACR (p value range 0.007-0.049); however, the association with the discrete trait albuminuria was not significant (p value range 0.094-0.338). We conclude that these variants in TJP1 do not appear to be major determinants for albuminuria in the SAFDGS; however, they may play a minor role in its severity in this Mexican-American population. Further examination of the TJP1 gene region in this and other cohorts will be useful to determine whether ZO-1 plays a significant role in glomerular permselectivity.

  14. Localization of vascular endothelial growth factor in the zona pellucida of developing ovarian follicles in the rat: a possible role in destiny of follicles.

    PubMed

    Celik-Ozenci, Ciler; Akkoyunlu, Gokhan; Kayisli, Umit Ali; Arici, Aydin; Demir, Ramazan

    2003-11-01

    There is increasing evidence that in many species angiogenic factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), may have important roles in folliculogenesis. The aim of this study is to determine the localization of VEGF and its receptors, Flt-1 and KDR, and bFGF expression in the rat ovary and to evaluate their distributions throughout the different follicular stages. Out of 20 virginal female rats, 10 were studied during the natural ovarian cycle without any ovulation induction. The other 10 were superovulated and their ovaries were studied by western analysis and immunohistochemistry. Granulosa cells (GC) and oocytes of primordial follicles were negative for VEGF. In early primary follicles, VEGF was present in the oocyte but its immunoreactivity was weak, while newly developing zona pellucida (ZP) of primary follicles was negative for VEGF. Subsequently, with the commencement of antral spaces between GC of the secondary follicle, ZP of some secondary follicles became strongly positive for VEGF, forming a continuous ring around the oocyte. In preovulatory mature follicles granulosa and theca interna (TI) cells showed a weak immunoreactivity for VEGF. Western blot analyses have also demonstrated that VEGF, a 26-kDa protein, was present in follicles. Moreover, in ovulated cumulus-oocyte complex we observed a halo-like immunoreactivity of VEGF around the fully mature oocyte. The immunoreactivity for Flt-1 and KDR receptors in growing follicles was mostly limited to GC and TI cells. Anti-bFGF did not exhibit any immunoreactivity in ZP of follicles at any stage. Its expression was weak in GC of the follicles at different stages, whereas, it could be localized to some extent in the blood capillaries of TI of antral follicles and in blood vessels localized in the stroma. Interestingly, VEGF immunoreactivity in the ZP of some secondary follicles is very striking. Accordingly, the possibility that VEGF may be an

  15. La contribución de los Centros Colaboradores de la OMS/OPS en los avances de la promoción de la salud en América Latina.

    PubMed

    Arroyo, Hiram V; Mantilla Uribe, Blanca P; Contreras Rengifo, Adolfo; Westphal, Marcia F; Mendes, Rosilda

    2016-09-01

    Los "Centros Colaboradores" de la Organización Mundial de la Salud (OMS) son instituciones designadas para prestar apoyo estratégico en el desarrollo de los objetivos y la ejecución de las actividades de la OMS, y para generar capacidad institucional en países y regiones. En las Américas existen ocho Centros Colaboradores directamente relacionados a la promoción de la salud. Cuatro centros se ubican en Norteamérica y los otros cuatro en América Latina. El propósito de este artículo es describir los Centros Colaboradores de la Región de América Latina. Los centros han sido importantes en el desarrollo de la agenda de promoción de la salud de la región, la consolidación de la estrategia de entornos saludables y el fortalecimiento de las redes académicas y profesionales de promoción de la salud. © The Author(s) 2015.

  16. CONFERENCE NOTE: CETO—Centro de Ciências e Tecnologias Opticas, Trends in Optical Fibre Metrology and Standards

    NASA Astrophysics Data System (ADS)

    1994-01-01

    requirements of the new generation of analogue and digital fibre optical systems, which require sophisticated measurement techniques employing complex instruments unique to optical measurements. The school will foster and enhance the interaction between material, devices, systems, and standards-oriented R&D communities, as well as between engineers concerned with design and manufacturers of systems and instrumentation. Topics Review of optical fibre communication technology and systems Measurement techniques for fibre characterization: Reliability and traceability Reference fibres and calibration artefacts Ribbon fibres Mechanical and environmental testing Fibre reliability Polarimetric measurements Passive components characterization: Splices and connectors Couplers, splitters, taps and WDMs Optical fibres and isolators WDM technologies and applications: WDM technologies Tunable optical filters Fibre amplifiers and sources: Performances and characterization Design and standards Nonlinear effects Subsystem design and standards: Design and fabrication techniques Performance degradation and reliability Evaluation of costs/performance/technology Sensors IR - optical fibres Plastic fibres Instrumentation Registration Participation free of charge for postgraduate students, with some grants available for travel and lodging expenses. All correspondence should be addressed to: Secretariat, Trends in Optical Fibre Metrology and Standards, a/c Prof. Olivério D D Soares, Centro de Ciências e Tecnologias Opticas, Lab. Fisica - Faculdade de Ciências, Praça Gomes Teixeira, P-4000 Porto, Portugal. Tel: 351-2-310290, 351-2-2001648; Fax: 351-2-319267.

  17. Investigating the relationship between climate teleconnection patterns and soil moisture variability in the Rio Grande/Río Bravo del Norte basin using the NOAH land surface model

    NASA Astrophysics Data System (ADS)

    Khedun, C. P.; Mishra, A. K.; Bolten, J. D.; Giardino, J. R.; Singh, V. P.

    2010-12-01

    Soil moisture is an important component of the hydrological cycle. Climate variability patterns, such as the Pacific Decadal Oscillation (PDO), El Niño Southern Oscillation (ENSO), and Atlantic Multidecadal Oscillation (AMO) are determining factors on surface water availability and soil moisture. Understanding this complex relationship and the phase and lag times between climate events and soil moisture variability is important for agricultural management and water planning. In this study we look at the effect of these climate teleconnection patterns on the soil moisture across the Rio Grande/Río Bravo del Norte basin. The basin is transboundary between the US and Mexico and has a varied climatology - ranging from snow dominated in its headwaters in Colorado, to an arid and semi-arid region in its middle reach and a tropical climate in the southern section before it discharges into the Gulf of Mexico. Agricultural activities in the US and in northern Mexico are highly dependent on the Rio Grande and are extremely vulnerable to climate extremes. The treaty between the two countries does not address climate related events. The soil moisture is generated using the community NOAH land surface model (LSM). The LSM is a 1-D column model that runs in coupled or uncoupled mode, and it simulates soil moisture, soil temperature, skin temperature, snowpack depth, snow water equivalent, canopy water content, and energy flux and water flux of the surface energy and water balance. The North American Land Data Assimilation Scheme 2 (NLDAS2) is used to drive the model. The model is run for the period 1979 to 2009. The soil moisture output is validated against measured values from the different Soil Climate Analysis Network (SCAN) sites within the basin. The spatial and temporal variability of the modeled soil moisture is then analyzed using marginal entropy to investigate monthly, seasonal, and annual variability. Wavelet transform is used to determine the relation, phase

  18. Hydrogen-rich Water Exerting a Protective Effect on Ovarian Reserve Function in a Mouse Model of Immune Premature Ovarian Failure Induced by Zona Pellucida 3

    PubMed Central

    He, Xin; Wang, Shu-Yu; Yin, Cheng-Hong; Wang, Tong; Jia, Chan-Wei; Ma, Yan-Min

    2016-01-01

    Background: Premature ovarian failure (POF) is a disease that affects female fertility but has few effective treatments. Ovarian reserve function plays an important role in female fertility. Recent studies have reported that hydrogen can protect male fertility. Therefore, we explored the potential protective effect of hydrogen-rich water on ovarian reserve function through a mouse immune POF model. Methods: To set up immune POF model, fifty female BALB/c mice were randomly divided into four groups: Control (mice consumed normal water, n = 10), hydrogen (mice consumed hydrogen-rich water, n = 10), model (mice were immunized with zona pellucida glycoprotein 3 [ZP3] and consumed normal water, n = 15), and model-hydrogen (mice were immunized with ZP3 and consumed hydrogen-rich water, n = 15) groups. After 5 weeks, mice were sacrificed. Serum anti-Müllerian hormone (AMH) levels, granulosa cell (GC) apoptotic index (AI), B-cell leukemia/lymphoma 2 (Bcl-2), and BCL2-associated X protein (Bax) expression were examined. Analyses were performed using SPSS 17.0 (SPSS Inc., Chicago, IL, USA) software. Results: Immune POF model, model group exhibited markedly reduced serum AMH levels compared with those of the control group (5.41 ± 0.91 ng/ml vs. 16.23 ± 1.97 ng/ml, P = 0.033) and the hydrogen group (19.65 ± 7.82 ng/ml, P = 0.006). The model-hydrogen group displayed significantly higher AMH concentrations compared with that of the model group (15.03 ± 2.75 ng/ml vs. 5.41 ± 0.91 ng/ml, P = 0.021). The GC AI was significantly higher in the model group (21.30 ± 1.74%) than those in the control (7.06 ± 0.27%), hydrogen (5.17 ± 0.41%), and model-hydrogen groups (11.24 ± 0.58%) (all P < 0.001). The GC AI was significantly higher in the model-hydrogen group compared with that of the hydrogen group (11.24 ± 0.58% vs. 5.17 ± 0.41%, P = 0.021). Compared with those of the model group, ovarian tissue Bcl-2 levels increased (2.18 ± 0.30 vs. 3.01 ± 0.33, P = 0.045) and the Bax

  19. Cerros del Norte Conservation Act

    THOMAS, 113th Congress

    Sen. Udall, Tom [D-NM

    2013-02-07

    Senate - 06/27/2013 Placed on Senate Legislative Calendar under General Orders. Calendar No. 108. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  20. PASO DEL NORTE ENVIRONMENTAL MONITOR

    EPA Science Inventory

    This project is developing an Internet web site and associated community linkages to provide relevant-time air quality and local traffic and transit information, in English and Spanish, to the international communities of El Paso, Texas, Sunland Park, New Mexico and Ciud...

  1. [Prevention rather than cure: the emergence and first stage of the Centros de Higiene Infantil in Mexico City, 1922-1932].

    PubMed

    Alanís, Mercedes

    2015-01-01

    This article deals with the main features of the emergence and first ten years of the Centros de Higiene Infantil, facilities run by the Departamento de Salubridad Pública from 1922 on in Mexico City with the goal of providing care for mothers from pregnancy onwards and children from birth to two years of age. It reviews the actions that gave rise to this project and how it became established. It analyzes the structure of these centers, the characteristics of the mothers and children seen there and the functions performed by doctors and nurses, stressing the notion of preventing childhood illnesses, and ends with a first assessment of the effects and limitations of these centers.

  2. Centro-Apical Self-Organization of Organic Semiconductors in a Line-Printed Organic Semiconductor: Polymer Blend for One-Step Printing Fabrication of Organic Field-Effect Transistors

    PubMed Central

    Jin Lee, Su; Kim, Yong-Jae; Young Yeo, So; Lee, Eunji; Sun Lim, Ho; Kim, Min; Song, Yong-Won; Cho, Jinhan; Ah Lim, Jung

    2015-01-01

    Here we report the first demonstration for centro-apical self-organization of organic semiconductors in a line-printed organic semiconductor: polymer blend. Key feature of this work is that organic semiconductor molecules were vertically segregated on top of the polymer phase and simultaneously crystallized at the center of the printed line pattern after solvent evaporation without an additive process. The thickness and width of the centro-apically segregated organic semiconductor crystalline stripe in the printed blend pattern were controlled by varying the relative content of the organic semiconductors, printing speed, and solution concentrations. The centro-apical self-organization of organic semiconductor molecules in a printed polymer blend may be attributed to the combination of an energetically favorable vertical phase-separation and hydrodynamic fluids inside the droplet during solvent evaporation. Finally, a centro-apically phase-separated bilayer structure of organic semiconductor: polymer blend was successfully demonstrated as a facile method to form the semiconductor and dielectric layer for OFETs in one- step. PMID:26359068

  3. Centro-Apical Self-Organization of Organic Semiconductors in a Line-Printed Organic Semiconductor: Polymer Blend for One-Step Printing Fabrication of Organic Field-Effect Transistors.

    PubMed

    Lee, Su Jin; Kim, Yong-Jae; Yeo, So Young; Lee, Eunji; Lim, Ho Sun; Kim, Min; Song, Yong-Won; Cho, Jinhan; Lim, Jung Ah

    2015-09-11

    Here we report the first demonstration for centro-apical self-organization of organic semiconductors in a line-printed organic semiconductor: polymer blend. Key feature of this work is that organic semiconductor molecules were vertically segregated on top of the polymer phase and simultaneously crystallized at the center of the printed line pattern after solvent evaporation without an additive process. The thickness and width of the centro-apically segregated organic semiconductor crystalline stripe in the printed blend pattern were controlled by varying the relative content of the organic semiconductors, printing speed, and solution concentrations. The centro-apical self-organization of organic semiconductor molecules in a printed polymer blend may be attributed to the combination of an energetically favorable vertical phase-separation and hydrodynamic fluids inside the droplet during solvent evaporation. Finally, a centro-apically phase-separated bilayer structure of organic semiconductor: polymer blend was successfully demonstrated as a facile method to form the semiconductor and dielectric layer for OFETs in one- step.

  4. Evaluation of emission control strategies to reduce ozone pollution in the Paso del Norte region using a photochemical air quality modeling system

    NASA Astrophysics Data System (ADS)

    Valenzuela, Victor Hugo

    variability of the PREDICTED to OBSERVED ozone concentrations of both BASELINE model and simulations with modified emissions assessed by the sensitivity analysis. All simulations were found to vary within acceptable ranges of these two criteria variables. Simulation results indicate ozone formation in the PdN region is VOC-limited. Under VOC-limited conditions, modifications to NOx emissions do not produce a marked increase or decrease in ozone concentrations. Modifications to VOC emissions generated the highest variability in ozone concentrations. Increasing VOC emissions by 75% produced results which minimized model bias and error when comparing PREDICTED and OBSERVED ozone concentrations. Increasing VOC emissions by 75% either alone or in combination with a 75% increase in NOx emissions generated PREDICTED ozone concentrations very near to OBSERVED ozone. By evaluating the changes in ambient ozone concentrations through photochemical modeling, air quality planners may identify the most efficient or effective VOC emissions control strategies for area sources. Among the strategies to achieve emissions reductions are installation of gasoline vapor recovery systems, replacing high-pressure low-volume surface coating paint spray guns with high-volume low-pressure spray paint guns, requiring emissions control booths for surface coating operations as well as undertaking solvent management practices, requiring the sale of low VOC paint solvents in the surface-coating industry, and requiring low-VOC solvents in the dry cleaning industry. Other strategies to reduce VOC emissions include initiating Eco-Driving strategies to reduce fuel consumption from mobile sources and minimize vehicle idling at the international ports of entry by reducing bridge wait times. This dissertation depicts a tool for evaluating impacts of emissions on regional air quality by addressing the highly unresolved fugitive emissions in the Paso del Norte region. It provides a protocol for decision makers to

  5. Use of geostatistics for assessing the concentration of heavy metals in a stretch of the River Apodi-Mossoro (Rio Grande do Norte State, Brazil).

    NASA Astrophysics Data System (ADS)

    Bezerra, J. M.; Siqueira, G. M.; Montenegro, A. A. A.; Silva, P. C. M.; Batista, R. O.

    2012-04-01

    The objective of this study was to assess the environmental changes with respect to the concentration of heavy metals in the sediment contained a stretch of the River Apodi-Mossoró (Rio Grande do Norte State, Brazil), considering changes in land use and soil. The sediment samples were collected at 30 points in the bed Apodi- Mossoró River in a section with features urban-rural town of Mossoró. The concentration of heavy metals in the sediment was determined using composite samples of surface sediments from the bottom with a depth of 20 cm, according to the methodology of APHAAWWA-WPCF (1998), where he subsequently held to determine the presence and quantity of metal concentration total by the technique of atomic absorption spectrometry, and analyzed the following heavy metals: aluminum(Al), cádmium (Cd), chromium (Cr), copper (Cu), iron (Fe), manganese (Mn), nickel (Ni), lead (Pb) and zinc (Zn). Data were analyzed using statistical and geostatistical. The geostatistical analysiswas performed by the construction of experimental semivariogramas self-assessment and adjustment by using the technique of Jack-kinifing. The elemento Cd was absent in the samples, which reduces the possibility of environmental contamination events. The average concentrations of the elements under study are within the limits proposed by the environmental legislation (National Environmental Council). However, for the elements Fe, Al and Mn no threshold values, because these are associated with the rocky material of geochemical origin. The elemento Fe had the highest range of values than the other, and all elements except for Zn and Cd showed the presence of outliers, suggesting the possibility that these points are listed as points liable to contribution by human activities. It was verified the presence of human influence, because the elements undergo an increase of concentration values from the point 11, which is located downstream of the urban bus consolidated. The experimental

  6. The human is an exception to the evolutionarily-conserved phenomenon of pre-fertilization zona pellucida resistance to proteolysis induced by oviductal fluid.

    PubMed

    Mondéjar, I; Avilés, M; Coy, P

    2013-03-01

    Is zona pellucida (ZP) resistance to proteolysis, induced by oviductal fluid (OF), a mechanism common to species other than the pig and cow? ZP resistance to proteolysis induced by OF was observed in the mouse, rat, hamster, rabbit, sheep, goat, pig and cow, but not in humans. Oviductal ZP resistance to proteolysis occurs in the pig and cow where it influences the incidence of fertilization and polyspermy. The effect is observed after incubation of ZP in OFs from pig (pOF), cow (cOF), rabbit (rOF) and sheep (sOF). Oocytes from nine different species, including ungulates, rodents, lagomorphs and primates were incubated in rOF, sOF, gOF, cOF, pOF and human oviductal fluid (hOF). ZP digestion times for the matured oocytes of these nine species, without any treatment or incubated in 5 (mouse, rat, hamster, rabbit, cow, ewe and goat) or 6 (pig and humans) of the OFs collected were compared using three replicates per treatment and at least three oocytes per replicate. In vivo matured oocytes from rat, hamster, mouse, rabbit and humans, in vitro matured oocytes from cow, goat, ewe and pig and rOF, cOF, gOF, sOF, pOF and human (hOF) were collected and processed for the study. Oocytes from each species were incubated in the different OFs for 30 min. The resistance of the ZP of the oocytes to enzymatic digestion in a pronase solution (0.5% in PBS) was measured and registered as ZP digestion time. rOF increased ZP resistance to proteolytic digestion in the range of between 96 and 720 h for any of the species tested, whereas the corresponding increase in human ZP was only 1 min. OFs from the remaining species also had a significant effect, with variations among the cross-species experiments (P < 0.05). hOF, which was only tested on human and porcine oocytes, had no effect on ZP chemical hardening. Measurements of ZP digestion times are not of extreme accuracy and errors of a few seconds can be assumed in the experimental data. However, when differences are in the range of

  7. The roles of protein disulphide isomerase family A, member 3 (ERp57) and surface thiol/disulphide exchange in human spermatozoa-zona pellucida binding.

    PubMed

    Wong, Chi-Wai; Lam, Kevin K W; Lee, Cheuk-Lun; Yeung, William S B; Zhao, Wei E; Ho, Pak-Chung; Ou, Jian-Ping; Chiu, Philip C N

    2017-04-01

    Are multimeric sperm plasma membrane protein complexes, ERp57 and sperm surface thiol content involved in human spermatozoa-zona pellucida (ZP) interaction? ERp57 is a component of a multimeric spermatozoa-ZP receptor complex involved in regulation of human spermatozoa-ZP binding via up-regulation of sperm surface thiol content. A spermatozoon acquires its fertilization capacity within the female reproductive tract by capacitation. Spermatozoa-ZP receptor is suggested to be a composite structure that is assembled into a functional complex during capacitation. Sperm surface thiol content is elevated during capacitation. ERp57 is a protein disulphide isomerase that modulates the thiol-disulphide status of proteins. The binding ability and components of protein complexes in extracted membrane protein fractions of spermatozoa were studied. The roles of capacitation, thiol-disulphide reagent treatments and ERp57 on sperm functions and sperm surface thiol content were assessed. Spermatozoa were obtained from semen samples from normozoospermic men. Human oocytes were obtained from an assisted reproduction programme. Blue native polyacrylamide gel electrophoresis, western ligand blotting and mass spectrometry were used to identify the components of solubilized ZP/ZP3-binding complexes. The localization and expression of sperm surface thiol and ERp57 were studied by immunostaining and sperm surface protein biotinylation followed by western blotting. Sperm functions were assessed by standard assays. Several ZP-binding complexes were isolated from the cell membrane of capacitated spermatozoa. ERp57 was a component of one of these complexes. Capacitation significantly increased the sperm surface thiol content, acrosomal thiol distribution and ERp57 expression on sperm surface. Sperm surface thiol and ERp57 immunoreactivity were localized to the acrosomal region of spermatozoa, a region responsible for ZP-binding. Up-regulation of the surface thiol content or ERp57 surface

  8. Zona pellucida-binding protein 2 (ZPBP2) and several proteins containing BX7B motifs in human sperm may have hyaluronic acid binding or recognition properties.

    PubMed

    Torabi, F; Bogle, O A; Estanyol, J M; Oliva, R; Miller, D

    2017-12-01

    Are there novel hyaladherins in human sperm? Zona pellucida-binding protein 2 (ZPBP2), containing a Link-like hyaluronic acid (HA)-binding domain, and several other proteins containing BX7B motifs, such as ADAM32 and Midkine, may be novel hyaladherins with HA-binding properties. HA-binding proteins (hyaladherins), which can bind HA surrounding the cumulus-oophorus complex, are distinct from hyases such as PH 20 (SPAM1) and are expressed by mature spermatozoa. Although HABP1 and CD44 are reasonably well characterized hyaladherins and the former has been implicated in sperm-oocyte interactions, the overall significance of sperm hyaladherins for male fertility is still poorly understood. This was a laboratory-based investigation into human sperm hyaladherins undertaken as part of a three year PhD programme sponsored by the EU Marie Curie Training network, Reprotrain. Protein homogenates of sperm obtained from young men of unknown fertility (N = 4) were partitioned into HA-binding and non-binding fractions by a protein affinity 'panning' method; their subsequent characterization was by liquid chromatography-tandem mass spectrometry (LC-MS-MS) and partitioning behaviour was confirmed by western blotting. Sequences of proteins from both fractions were submitted to PDBsum to look for orthologous entries (PDB codes) and all returned codes were queried against the matching protein using SAS (Sequences Annotated by Structure) looking for structural similarities between them. A systematic search for other common features of hyaladherins was also undertaken. The presence of BX7B sequence motifs found in several well-described hyaladherins including RHAMM was used to assess efficacy of potential hyaladherin partitioning by the HA substrate. The data showed that 50% (14/28) and 34.5% (28/81) of proteins in the bound and unbound fractions, respectively, contained these motifs (one-tailed Z-score = 1.45; P = 0.074), indicating weak discrimination by the substrate. Querying PDBsum

  9. On the occurrence of Cebus flavius (Schreber 1774) in the Caatinga, and the use of semi-arid environments by Cebus species in the Brazilian state of Rio Grande do Norte.

    PubMed

    Ferreira, Renata G; Jerusalinsky, Leandro; Silva, Thiago César Farias; de Souza Fialho, Marcos; de Araújo Roque, Alan; Fernandes, Adalberto; Arruda, Fátima

    2009-10-01

    Cebus flavius is a recently rediscovered species and a candidate for the 25 most endangered primate species list. It was hypothesized that the distribution of C. flavius was limited to the Atlantic Forest, while the occurrence of C. libidinosus in the Rio Grande do Norte (RN) Caatinga was inferred, given its occurrence in neighboring states. As a result of a survey in ten areas of the RN Caatinga, this paper reports on four Cebus populations, including the first occurrence of C. flavius in the Caatinga, and an expansion of the northwestern limits of distribution for the species. This C. flavius population may be a rare example of a process of geographic distribution retraction, and is probably the most endangered population of this species. New areas of occurrence of C. libidinosus are also described. Tool use sites were observed in association with reports of the presence of both capuchin species.

  10. Evaluacion de los recursos potenciales del petroleo y gas, en Centro y Suramerica [Evaluation of potential petroleum and gas resources in Central and South America

    USGS Publications Warehouse

    Schenk, C.S.

    2001-01-01

    El Servicio Geológico de los Estados Unidos (USGS, por sus siglas en inglés) completó recientemente un estudio evaluativo de recursos potenciales de petróleo y gas en 130 provincias de petróleo seleccionadas en diferentes partes del mundo (USGS, 2000). De estas 130 provincias, 23 se encuentran en Suramérica, Centroamérica, y la región del Caribe (fig. 1). El estudio comprendió desde las provincias de petróleo establecidas con un largo historial de producción, como la Cuenca de Maracaibo, hasta las provincias fronterizas de poca o ninguna producción, como la Cuenca de Guyana-Suriname. No todas las provincias con historial de producción o con potencial de producción fueron evaluadas en el Estudio Evaluativo USGS 2000. Al presente, el USGS está evaluando muchas de las provincias restantes de petróleo y gas, en Centro y Suramérica. En cada provincia hemos (1) definido geológicamente el total de los sistemas de petróleo, (2) definido las unidades evaluadas que forman parte de todos los sistemas de petróleo, y (3) evaluado el volumen potencial de petróleo y gas convencional en cada unidad evaluada. Definimos un total de 26 sistemas de petróleo y 55 unidades evaluadas en las 23 provincias

  11. Factors associated with failure of clinical screening among blood donors who have altered serological results in the Centro Regional de Hemoterapia de Ribeirão Preto

    PubMed Central

    Ferreira, Oranice; Passos, Afonso Dinis Costa

    2012-01-01

    Objective This study aimed to investigate the frequency of positive results for hepatitis B and C, HIV and syphilis in blood donations at the Centro Regional de Hemoterapia de Ribeirão Preto, to describe donors with positive results according to some demographic and socioeconomic variables, to identify risk factors associated to these donors and the reasons that they were not detected during clinical screening. Methods A descriptive study was performed between July 1st 2005 and July 31st 2006 by interviewing 106 donorsafter medical consultations where they were informed of positive results for hepatitis B, hepatitis C, HIV or syphilis. Results There was a predominance of first-time donors, males, under 50-year olds, married individuals, from Ribeirão Preto, with elementary education, low economic status and of people who donated at the request of friends or relatives. Hepatitis C was the most frequently detected infection (56.6%), followed by hepatitis B (20.7%), HIV (12.3%) and syphilis(10.4%). About 40% of donors had omitted risk factors for different reasons: because they trusted the results of serological tests, did not feel comfortable about talking of risk factors or did not consider them relevant. Other justifications were the duration of the interview, the interviewer was unskilled, embarrassment and doubts about confidentiality. Conclusion The results indicate the need for changes in the approach to clinical screening and a review of methods to attract and guide potential donors. PMID:23323063

  12. Activities of the International Radio Medical Centre (C.I.R.M.) in Rome during the last five years (1996-2000). Centro Internazionale Radio Medico.

    PubMed

    Amenta, F; Dauri, A

    2001-01-01

    This paper reviews medical activity provided from 1996 to 2000 by the International Radio Medical Centre (Centro Internazionale Radio Medico, C.I.R.M.). C.I.R.M. is a non-profit organization headquartered in Rome and providing freely telemedical advice to ships flying of any flag navigating on all seas of the world, to civil airplanes and to small Italian islands. From 1996 to 2000 C.I.R.M. has assisted 4,982 patients, 4,686 of which (94%) on board ships, 85 on airplanes and 206 on small Italian islands. More than 65% of requests of telemedical advice received by C.I.R.M. were from non-Italian ships. This indicates the really international nature of C.I.R.M.'s activity. The largest number of medical requests come from the Atlantic Ocean, followed by the Mediterranean sea, Indian Ocean and Pacific Ocean. In terms of pathologies assisted, accidents took the first place, followed as main pathologies by gastrointestinal disorders, cardiovascular pathologies, respiratory disorders, infectious and parasitic diseases and nervous system complaints. Analysis of the outcome of C.I.R.M.'s medical activity showed that more than 50% of patients assisted were recovered or improved while assisted by the Centre.

  13. Misura della sezione d'urto totale e Dello scattering elastico all'energia Nel centro di massa di $$\\sqrt{s}=1.8$$ TeV (in Italian)

    SciTech Connect

    Paoletti, Riccardo

    La produzione di eventi elastici è stata misurata al Collider Tevatron del Fermi National Accelerator Laboratory (Batavia. 11.~ U .S.A.) alla energia nel centro di massa. di √s = 1800 GeV, nell'intervallo 0.06 < I t I< 0.24. Sono forniti dettagli sull'esperimento CDF che ha compiuto la misura e sui criteri di selezione degli eventi.

  14. [Prevalence of American trypanosomiasis, syphilis, toxoplasmosis, rubella, hepatitis B, hepatitis C, human immunodeficiency virus infection, assayed through serological tests among pregnant patients, from 1996 to 1998, at the Regional University Hospital Norte do Paraná].

    PubMed

    Reiche, E M; Morimoto, H K; Farias, G N; Hisatsugu, K R; Geller, L; Gomes, A C; Inoue, H Y; Rodrigues, G; Matsuo, T

    2000-01-01

    In order to evaluate the seroprevalence of the american trypanosomiasis, syphilis, toxoplasmosis, rubella, hepatitis B infection, hepatitis C infection and human immunodeficiency virus infection among pregnant women attended at the Hospital Universitário Regional Norte do Paraná, Londrina State University, Paraná, a retrospective study of the serologic results performed in the prenatal routine during the period of June 1996 to June 1998 was carried out. The rates of seropositivity were as follows: american trypanosomiasis = 0.9%, syphilis = 1.6%, toxoplasmosis = 67% (IgG) and 1.8% (IgM), rubella = 89% (IgG) and 1.2% (IgM), hepatitis B surface antigen = 0.8%, hepatitis C virus = 0.8% and human immunodeficiency virus infection = 0.6%. An association between the increase in the seroprevalence of Chagas' disease and patient age was detected (p=0.006). The results underscore the importance of the serological tests in perinatal care, to prevent both the congenital and perinatally transmitted forms of theses infectious diseases.

  15. Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 3: developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane.

    PubMed

    Hermo, Louis; Pelletier, R-Marc; Cyr, Daniel G; Smith, Charles E

    2010-04-01

    Spermiogenesis constitutes the steps involved in the metamorphosis of spermatids into spermatozoa. It involves modification of several organelles in addition to the formation of several structures including the flagellum and cytoplasmic droplet. The flagellum is composed of a neck region and middle, principal, and end pieces. The axoneme composed of nine outer microtubular doublets circularly arranged to form a cylinder around a central pair of microtubules is present throughout the flagellum. The middle and principal pieces each contain specific components such as the mitochondrial sheath and fibrous sheath, respectively, while outer dense fibers are common to both. A plethora of proteins are constituents of each of these structures, with each playing key roles in functions related to the fertility of spermatozoa. At the end of spermiogenesis, a portion of spermatid cytoplasm remains associated with the released spermatozoa, referred to as the cytoplasmic droplet. The latter has as its main feature Golgi saccules, which appear to modify the plasma membrane of spermatozoa as they move down the epididymal duct and hence may be partly involved in male gamete maturation. The end product of spermatogenesis is highly streamlined and motile spermatozoa having a condensed nucleus equipped with an acrosome. Spermatozoa move through the female reproductive tract and eventually penetrate the zona pellucida and bind to the egg plasma membrane. Many proteins have been implicated in the process of fertilization as well as a plethora of proteins involved in the development of spermatids and sperm, and these are high lighted in this review. Copyright 2009 Wiley-Liss, Inc.

  16. Evaluation of surrogate markers for human immunodeficiency virus infection among blood donors at the blood bank of "Hospital Universitário Regional Norte do Paraná", Londrina, PR, Brazil.

    PubMed

    Reiche, Edna Maria Vissoci; Vogler, Ingridt Hildegard; Morimoto, Helena Kaminami; Bortoliero, André Luis; Matsuo, Tiemi; Yuahasi, Kátia Kioko; Cancian, Sanderson Júnior; Koguichi, Roberto Setsuo

    2003-01-01

    This study evaluated the usefulness of the anti-HBc, hepatitis C virus antibodies (anti-HCV), human T cell lymphotropic virus I and II antibodies (anti-HTLV I/II), serologic tests for syphilis, and surface antigen of hepatitis B virus (HBsAg) as surrogate markers for the risk for HIV infection in 80,284 serum samples from blood donors from the Blood Bank of "Hospital Universitário Regional Norte do Paraná", Londrina, Paraná State, Brazil, analyzed from July 1994 to April 2001. Among 39 blood donors with positive serology for HIV, 12 (30.8%) were anti-HBc positive, 10 (25.6%) for anti-HCV, 1 (2.6%) for anti-HTLV I/I, 1 (2.6%) was positive for syphilis, and 1 (2.6%) for HBsAg. Among the donors with negative serology for HIV, these markers were detected in 8,407 (10.5%), 441 (0.5%), 189 (0.2%), 464 (0.6%), and 473 (0.6%) samples, respectively. The difference was statistically significant (p < 0.001) for anti-HBc and anti-HCV. Although the predictive positive values for these surrogate markers were low for HIV infection, the results confirmed the anti-HBc and anti-HCV as useful surrogate markers for HIV infection thus reinforcing the maintenance of them in the screening for blood donors contributing to the prevention of the small number of cases in which HIV is still transmitted by transfusion.

  17. [A study on the construction, expression and immunosterility of Lagurus laguru zona pellucida 3 DNA vaccine pVAX1-sig-LTB-lZP3-C3d3].

    PubMed

    Li, Chen-Chen; Yu, Ji-Yun; Jiang, Min; Tu, Yi-Xian; Ma, Xiao-Lin; Zhang, Fu-Chun

    2011-09-01

    To enhance the immunocontraceptive effect of Lagurus lagurus zona pellucida 3 DNA vaccine, and to achieve the prospect of application through the pVAX1-sig-LTB-lZP3-C3d3 different immunity pathway. Two adjuvant molecules were constructed into the recombinant plasmid pVAX1-sig-LTB-lZP3-C3d3 as DNA vaccine which contains Escherichia coli heat-labile enterotoxin B subunit and the molecular adjuvant 3 copies of C3d. The results of RT-PCR and western blot showed that the DNA vaccine was expressed in mRNA and protein level. The female C57BL/6 mice were immunized by three ways: intramuscular injection, intranasal or oral route.Antibody levels and types were detected by ELISA. ELISA results showed that recombinant plasmid pVAX1-sig-LTB-lZP3-C3d3 immunization induced specific IgG, IgA levels were significantly different comparing with control (P<0.01). Antifertility experiment showed that the experimental group reduced the average fertility significantly different compared with the control group (P<0.01). Restriction analysis, RT-PCR and Western blot showed that the recombinant plasmid constructed correctly and can be the expression of mRNA and protein levels.It resulted that the recombinant plasmid pVAX1-sig-LTB-lZP3-C3d3 can induce the specific immune response efficiently and enhance the immunocontraceptive effects.

  18. Melanin-concentrating hormone and neuropeptide EI projections from the lateral hypothalamic area and zona incerta to the medial septal nucleus and spinal cord: a study using multiple neuronal tracers.

    PubMed

    Bittencourt, J C; Elias, C F

    1998-09-14

    The projection pathways of neurons containing melanin-concentrating hormone (MCH) and neuropeptide EI (NEI), two peptides colocalized in the lateral hypothalamic area (LHA) of the rat, were mapped using the retrogradely transported fluorescent dyes, true blue (TB) and diamidino yellow (DY). TB and DY were injected into the medial septum/diagonal band complex (MS/DBC) and the thoracic level of the spinal cord (SpCd), respectively. Brains from rats receiving only one or both tracer injections were immunohistochemically stained for MCH in the spinal cord and NEI in the forebrain. In the MS/DBC, NEI-immunoreactive (-ir) fibers are concentrated in the MS and in the vertical and horizontal limbs of the DBC. In the SpCd, MCH-ir fibers are concentrated primarily in lamina X. Of the diencephalic NEI-ir neurons, 37.15% project to the MS/DBC and reside in the rostromedial zona incerta (ZIm), in the LHAt and LHAp, and in the perifornical region. Of the diencephalic MCH-ir neurons, 20.2% project to the SpCd and reside in the LHAt and LHAp. In addition, 2. 2% of the MCH-ir cells and 8.7% of the NEI-ir cells in the hypothalamus were labeled with both retrograde tracers and thus project to both the MS/DBC and SpCd. These dual projection neurons are located mainly in the LHAt and LHAp. Anterograde injections of the tracer Phaseolus vulgaris leucoagglutinin into the LHAt and ZIm corroborated our findings in the retrograde studies. Potential autonomic and behavioral roles of the NEI and MCH systems in the MS/DBC and the SpCd are discussed. Copyright 1998 Elsevier Science B. V.

  19. [In Process Citation].

    PubMed

    Cuerda, Cristina; Álvarez, Julia; Ramos, Primitivo; Abánades, Juan Carlos; García-de-Lorenzo, Abelardo; Gil, Pedro; De-la-Cruz, Juan José

    2016-03-25

    Introducción: la desnutrición relacionada con la enfermedad (DRE) es un problema sociosanitario frecuente que afecta preferentemente a los mayores de 65 años, que aumenta la morbimortalidad y disminuye la calidad de vida.  Objetivo: estudiar la prevalencia de DRE en mayores de 65 años en diferentes centros sociosanitarios del Servicio Regional de Bienestar Social de la Comunidad de Madrid. Métodos: estudio transversal en 33 centros sociosanitarios de Madrid (6 centros de atención primaria [AP], 9 centros de mayores [CM], 9 hospitales [H] y 9 residencias [R]) seleccionados mediante muestreo polietápico. Las variables estudiadas fueron edad, sexo, nivel de dependencia según la escala de incapacidad de la Cruz Roja, motivo de ingreso y enfermedad de base, hábitat (urbano-periurbano-rural) y distribución geográfica (norte centro-sur). Como herramienta de cribado nutricional se utilizó el Mini Nutritional Assessment (MNA-cribaje) en todos los centros. En los pacientes con cribado positivo (en riesgo-desnutrición) se realizó el MNA-evaluación. El estudio estadístico se realizó con el paquete SSS 21.0 e incluyó estadística descriptiva, test de Chi-cuadrado y prueba exacta de Fisher, ANOVA de un factor, Kruskal-Wallis y análisis de regresión logística (RL) binaria univariante y multivariante. Se consideró significación estadística p < 0,05. Resultados: se reclutaron 1.103 sujetos (275 AP, 278 CM, 281 H, 269 R), edad media de 79,5 ± 8,4 años (41,2% varones, 58,8% mujeres). Los sujetos procedentes de H y R tuvieron un mayor grado de incapacidad (p < 0,001). La prevalencia global de DRE fue del 10%, encontrándose un 23,3% en riesgo de desnutrición, con diferencias entre los cuatro tipos de centros sociosanitarios (p < 0,001). El análisis univariante de RL mostró diferencia significativas en la prevalencia de desnutrición según la edad, sexo, grado de dependencia, tipo de centro sociosanitario, hábitat y zona geográfica. Sin embargo, en el

  20. Streamflow gains and losses and selected water-quality observations in five subreaches of the Rio Grande/Rio Bravo del Norte from near Presidio to Langtry, Texas, Big Bend area, United States and Mexico, 2006

    USGS Publications Warehouse

    Raines, Timothy H.; Turco, Michael J.; Connor, Patrick J.; Bennett, Jeffery B.

    2012-01-01

    Few historical streamflow and water-quality data are available to characterize the segment of the Rio Grande/Rio Bravo del Norte (hereinafter Rio Grande) extending from near Presidio to near Langtry, Texas. The U.S. Geological Survey, in cooperation with the National Park Service and the Texas Commission on Environmental Quality, collected water-quality and streamflow data from the Rio Grande from near Presidio to near Langtry, Texas, to characterize the streamflow gain and loss and selected constituent concentrations in a 336.3-mile reach of the Rio Grande from near Presidio to near Langtry, Texas. Streamflow was measured at 38 sites and water-quality samples were collected at 20 sites along the Rio Grande in February, March, and June 2006. Streamflow gains and losses over the course of the stream were measured indirectly by computing the differences in measured streamflow between sites along the stream. Water-quality data were collected and analyzed for salinity, dissolved solids, major ions, nutrients, trace elements, and stable isotopes. Selected properties and constituents were compared to available Texas Commission on Environmental Quality general use protection criteria or screening levels. Summary statistics of selected water-quality data were computed for each of the five designated subreaches. Streamflow gain and loss and water-quality constituent concentration were compared for each subreach, rather than the entire segment because of the temporal variation in sample collection caused by controlled releases upstream. Subreach A was determined to be a losing reach, and subreaches B, C, D, and E were determined to be gaining reaches. Compared to concentrations measured in upstream subreaches, downstream subreaches exhibited evidence of dilution of selected constituent concentrations. Subreaches A and B had measured total dissolved solids, chloride, and sulfate exceeding the Texas Commission on Environmental Quality general use protection criteria

  1. Node Ranking Tool - NoRT

    DTIC Science & Technology

    2018-03-23

    Unclassified Unlimited Unclassified Unlimited Unclassified Unlimited 23 Ira S. Moskowitz (202) 404-7930 This paper gives a description of the Node Ranking Tool...Disease, Virus, Expectation, Pandemic, Close- ness, Graph, Degree, Spectrum. I. INTRODUCTION THis paper gives a description of the Node Ranking Tool...is very much dependent upon which centrality measure we use. Therefore, following [6] and [3], we use TOPSIS to evaluate our decisions about the

  2. Medicina integrativa en América: De qué forma se está practicando la medicina integrativa en los centros clínicos en los Estados Unidos

    PubMed Central

    Horrigan, Bonnie; Lewis, Sheldon; Abrams, Donald I.; Pechura, Constance

    2012-01-01

    RESUMEN EJECUTIVO El impulso para desarrollar e implementar estrategias de medicina integrativa está enraizado en el deseo de mejorar la atención al paciente. The Bravewell Collaborative, una organización sin ánimo de lucro dedicada a la mejora de la atención sanitaria, define la medicina integrativa como “un enfoque de la medicina que coloca al paciente en el centro y se dirige al conjunto completo de influencias físicas, emocionales, mentales, sociales, espirituales y ambientales que afectan a la salud de la persona. Con una estrategia personalizada que considera las condiciones, necesidades y circunstancias únicas del paciente, utiliza las intervenciones más apropiadas de una variedad de disciplinas científicas para curar la afección y la enfermedad y ayudar a las personas a recobrar y mantener una salud óptima”. En las pasadas dos décadas, se ha documentado un número creciente de centros clínicos que proporcionan medicina integrativa, el número de facultades y escuelas médicas que enseñan estrategias integrativas, el número de investigadores que estudian intervenciones integrativas, y el número de pacientes que solicitan cuidados integrativos. Pero se desconocía si la medicina integrativa se estaba ofreciendo de manera igual, similar, o dispar. Además, mientras que los estudios anteriores se centraban en la prevalencia y el uso de la medicina complementaria o alternativa (MCA) por parte de los pacientes1,2 o de los médicos en hospitales3, enumerando la utilización de terapias MCA individuales, se había recogido muy poca información con respecto a la práctica real de la medicina integrativa que, por definición, trata a la persona en su conjunto. En 2011, The Bravewell Collaborative encargó una encuesta para determinar la forma en que la medicina integrativa se estaba practicando en los Estados Unidos: (1) describiendo las poblaciones de pacientes y las afecciones sanitarias tratadas más habitualmente; (2) definiendo las pr

  3. The new emergency structure of the Istituto Nazionale di Geofisica e Vulcanologia during the L’Aquila 2009 seismic sequence: the contribution of the COES (Seismological Emergency Operation Center - Centro Operativo Emergenza Sismica)

    NASA Astrophysics Data System (ADS)

    Moretti, M.; Govoni, A.; Nostro, C.; La Longa, F.; Crescimbene, M.; Pignone, M.; Selvaggi, G.; Working Group, C.

    2009-12-01

    The Centro Nazionale Terremoti (CNT - National Earthquake Center), departement of Istituto Nazionale di Geofisica e Vulcanologia (INGV), has designed and setup a rapid response emergency structure to face the occurrence of strong earthquakes. This structure is composed by a real time satellite telemetered temporary seismic network (see Abruzzese et al., 2009 Fall AGU) used to improve the hypocentral locations of the INGV National Seismic Network, a stand alone temporary seismic network whose goal is primarily the high dynamic/high resolution data acquisition in the epicentral area and a mobile operational center, the COES (Centro Operativo Emergenza Sismica, Seismological Emergency Operational Center). The COES structure is a sort of mobile office equipped with satellite internet communication that can be rapidly installed in the disaster area to support all the INGV staff operative needs and to cooperate with the Civil Protection department (DPC) aggregating all the scientific information available on the seismic sequence and providing updated information to Civil Protection for the decision making stage during the emergency. The structure is equipped with a heavy load trolley that carries a 6x6 inflatable tent, a satellite router, an UPS, computers, monitors and furniture. The facility can be installed in a couple of hours in the epicentral area and provides a full featured office with dedicated internet connection and VPN access to the INGV data management center in Rome. Just after the April 6 2009 Mw 6.3 earthquake in L’Aquila (Central Italy) the COES has been installed upon request of the Italian Civil Protection (DPC) in the DICOMAC (Directorate of Command and Control - which is the central structure of the DPC that coordinates the emergency activities in the areas affected by the earthquake) located in the Guardia di Finanza headquarters in Coppito nearby L'Aquila (the same location that hosted the G8 meeting). The COES produces real time reports on the

  4. FREQUENCY OF ANTI- Toxocara spp. ANTIBODIES IN INDIVIDUALS ATTENDED BY THE CENTRO DE SALUD FAMILIAR AND ENVIRONMENTAL CONTAMINATION WITH Toxocara canis EGGS IN DOG FECES, IN THE COASTAL NIEBLA TOWN, CHILE.

    PubMed

    Vargas, Catalina; Torres, Patricio; Jercic, María Isabel; Lobos, Marta; Oyarce, Alan; Miranda, Juan Carlos; Ayala, Salvador

    2016-09-22

    The frequency of anti-Toxocara spp. antibodies in individuals attended by the Centro de Salud Familiar in the coastal Niebla town, Chile, was related to the host and to environmental factors. IgG anti- Toxocara antibodies were detected with a commercial ELISA kit (SCIMEDX Corporation, USA). Samples with undetermined absorbance values were subjected to an additional ELISA standardized by the Instituto de Salud Pública, Chilean Health Ministry, a commercial ELISA (NOVATEC, Germany), and a commercial Western blot kit (LDBio Diagnostics, France). Hematological exams were performed using an automated blood counter and blood smears. Dog feces were collected from the ground along the main road in Niebla, including rural and urban locations. Ninety (25.4%) of the 355 examined individuals were positive by the ELISA test. The frequency of anti-Toxocara antibodies and the infection risk were significantly higher (p < 0.05) among those individuals ≥ 40 years old with respect to the 20-39 years old group, in individuals from rural locations, those who did not have a safe drinking water supply in the house or who presented blood eosinophilia. The proportion of positive samples of dog feces and the mean number of Toxocara canis eggs/g of feces in urban and rural areas were similar (p > 0.05).

  5. FREQUENCY OF ANTI- Toxocara spp. ANTIBODIES IN INDIVIDUALS ATTENDED BY THE CENTRO DE SALUD FAMILIAR AND ENVIRONMENTAL CONTAMINATION WITH Toxocara canis EGGS IN DOG FECES, IN THE COASTAL NIEBLA TOWN, CHILE

    PubMed Central

    VARGAS, Catalina; TORRES, Patricio; JERCIC, María Isabel; LOBOS, Marta; OYARCE, Alan; MIRANDA, Juan Carlos; AYALA, Salvador

    2016-01-01

    SUMMARY The frequency of anti-Toxocara spp. antibodies in individuals attended by the Centro de Salud Familiar in the coastal Niebla town, Chile, was related to the host and to environmental factors. IgG anti- Toxocara antibodies were detected with a commercial ELISA kit (SCIMEDX Corporation, USA). Samples with undetermined absorbance values were subjected to an additional ELISA standardized by the Instituto de Salud Pública, Chilean Health Ministry, a commercial ELISA (NOVATEC, Germany), and a commercial Western blot kit (LDBio Diagnostics, France). Hematological exams were performed using an automated blood counter and blood smears. Dog feces were collected from the ground along the main road in Niebla, including rural and urban locations. Ninety (25.4%) of the 355 examined individuals were positive by the ELISA test. The frequency of anti-Toxocara antibodies and the infection risk were significantly higher (p < 0.05) among those individuals ≥ 40 years old with respect to the 20-39 years old group, in individuals from rural locations, those who did not have a safe drinking water supply in the house or who presented blood eosinophilia. The proportion of positive samples of dog feces and the mean number of Toxocara canis eggs/g of feces in urban and rural areas were similar (p > 0.05). PMID:27680167

  6. Asociación de Hemato-Oncología Pediátrica de Centro América (AHOPCA): a model for sustainable development in pediatric oncology.

    PubMed

    Barr, Ronald D; Antillón Klussmann, Federico; Baez, Fulgencio; Bonilla, Miguel; Moreno, Belgica; Navarrete, Marta; Nieves, Rosa; Peña, Armando; Conter, Valentino; De Alarcón, Pedro; Howard, Scott C; Ribeiro, Raul C; Rodriguez-Galindo, Carlos; Valsecchi, Maria Grazia; Biondi, Andrea; Velez, George; Tognoni, Gianni; Cavalli, Franco; Masera, Giuseppe

    2014-02-01

    Bridging the survival gap for children with cancer, between those (the great majority) in low and middle income countries (LMIC) and their economically advantaged counterparts, is a challenge that has been addressed by twinning institutions in high income countries with centers in LMIC. The long-established partnership between a Central American consortium--Asociación de Hemato-Oncología Pediátrica de Centro América (AHOPCA)--and institutions in Europe and North America provides a striking example of such a twinning program. The demonstrable success of this endeavor offers a model for improving the health outcomes of children with cancer worldwide. As this remarkable enterprise celebrates its 15th anniversary, it is appropriate to reflect on its origin, subsequent growth and development, and the lessons it provides for others embarking on or already engaged in similar journeys. Many challenges have been encountered and not all yet overcome. Commitment to the endeavor, collaboration in its achievements and determination to overcome obstacles collectively are the hallmarks that stamp AHOPCA as a particularly successful partnership in advancing pediatric oncology in the developing world. © 2013 Wiley Periodicals, Inc.

  7. Construction of a manual of work processes and techniques from Centro de Dispensação de Medicamentos de Alto Custo (CEDMAC), Hospital de Clínicas, Unicamp.

    PubMed

    Bertolo, Manoel Barros; Ferreira, Bruno Silva de Araújo; Marchiore, Adriana G Mucke; Carvalho, Glaucia Pereira do Amaral; de Souza, Débora Pessoa; Psaltikidis, Eliane Molina

    2014-01-01

    The Centers for High Cost Medication (Centros de Medicação de Alto Custo, CEDMAC), Health Department, São Paulo were instituted by project in partnership with the Clinical Hospital of the Faculty of Medicine, USP, sponsored by the Foundation for Research Support of the State of São Paulo (Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP) aimed at the formation of a statewide network for comprehensive care of patients referred for use of immunobiological agents in rheumatological diseases. The CEDMAC of Hospital de Clínicas, Universidade Estadual de Campinas (HC-Unicamp), implemented by the Division of Rheumatology, Faculty of Medical Sciences, identified the need for standardization of the multidisciplinary team conducts, in face of the specificity of care conducts, verifying the importance of describing, in manual format, their operational and technical processes. The aim of this study is to present the methodology applied to the elaboration of the CEDMAC/HC-Unicamp Manual as an institutional tool, with the aim of offering the best assistance and administrative quality. In the methodology for preparing the manuals at HC-Unicamp since 2008, the premise was to obtain a document that is participatory, multidisciplinary, focused on work processes integrated with institutional rules, with objective and didactic descriptions, in a standardized format and with electronic dissemination. The CEDMAC/HC-Unicamp Manual was elaborated in 10 months, with involvement of the entire multidisciplinary team, with 19 chapters on work processes and techniques, in addition to those concerning the organizational structure and its annexes. Published in the electronic portal of HC Manuals in July 2012 as an e-Book (ISBN 978-85-63274-17-5), the manual has been a valuable instrument in guiding professionals in healthcare, teaching and research activities.

  8. Assessment of water resources potential of Ceará state (Brazil)

    NASA Astrophysics Data System (ADS)

    Araujo, Angelo; Pereira, Diamantino; Pereira, Paulo

    2016-04-01

    A methodological approach and results on water resources assessment in large areas are described with the case study of Ceará State (148,016 km2, northeast Brazil), where the scarceness of water resources is one of the main challenges in territorial planning and development. This work deals with the quantification and the mapping of water resources potential, being part of methodological approaches applied to the quantification of hydric diversity and geodiversity. Water resources potential is here considered as the sum of the hydric elements rainfall, groundwater specific discharge, water reservoirs, and river hierarchy. The assessment was based in a territorial organization by drainage sub-basins and in vector maps generated and treated with GIS software. Rainfall, groundwater specific discharge and hydrographical data were obtained in official institutions and allowed the construction of the annual mean rainfall map for a forty year period (1974-2014), the annual mean groundwater specific discharge map for a thirty-four year period, and the river and drainage basin hierarchy maps. These delivered rainfall, groundwater specific discharge, water reservoirs and river hierarchy partial indices expressed on quantitative maps with normalized values distributed by level 3 drainage basins. The sum of the partial indices originated the quantitative map of water resources potential index and by the Gaussian interpolation of this quantitative data a map of hydric diversity in Ceará state was created. Therefore, the water resources potential index is higher in 4 regions of the state (Noroeste Cearense, Zona Metropolitana de Fortaleza e da Zona Norte, Vale do Jaguaribe and Zonas Centro-sul e Sul Cearense). The index is low or very low in the whole region of Sertões Cearenses, confirming the important role of climatic features in hydrological diversity. Water resources management must consider technical tools for water resources assessment, in the line of other methods for

  9. Perfil das alterações vasculares periféricas em dependentes de crack acompanhados em Centro de Atenção Psicossocial para Álcool e Drogas (CAPS-AD)

    PubMed Central

    da Costa, Antônio Fagundes; Baldaçara, Leonardo Rodrigo; da Silva, Sílvio Alves; Tavares, Ana Célia de Freitas Ramos; Orsolin, Ederson de Freitas; Prehl, Vinícius Barros; Gondo, Fernando Hirohito Beltran; Santana, Hernani Lopes

    2016-01-01

    Resumo Contexto O consumo de crack é um dos grandes desafios em saúde pública, e o uso dessa droga tem efeitos diretos na saúde de seus usuários. Objetivos Avaliar o perfil das alterações vasculares em pacientes com dependência de crack em Centro de Atenção Psicossocial para Álcool e Drogas (CAPS-AD) e observar os possíveis efeitos vasculares periféricos. Métodos Trata-se de um estudo observacional, descritivo, de corte transversal. Os pacientes da amostra foram submetidos a um questionário objetivo para avaliar questões demográficas, padrão de uso da droga, coexistência de diabetes melito, hipertensão arterial ou tabagismo, exame físico e ecográfico. Os dados foram sumarizados e analisados estatisticamente com teste qui-quadrado ou teste exato de Fisher. Resultados A média de idade da amostra foi de 33,29 (±7,15) anos, e 74% eram do gênero masculino. A média de idade de início de uso da droga foi de 23,4 (±7,78) anos, com tempo médio de uso de 9,58 (±5,64) anos. O consumo médio diário de pedras de crack foi de 21,45 (±8,32) pedras. A alteração de pulsos em membros inferiores foi mais frequente em mulheres. A prevalência do espessamento da parede arterial nos membros inferiores foi de 94,8%. O tempo de uso da droga apresentou associação estatística (p = 0,0096) com alteração do padrão de curva espectral das artérias dos membros inferiores. Conclusões Há alterações vasculares periféricas em usuários de crack. O tempo de uso da droga exerceu um maior impacto nesse sistema, o que sugere associação entre o uso do crack e a diminuição de fluxo arterial.

  10. Prevalencia de síntomas depresivos e invarianza factorial de la Escala de Depresión del Centro de Estudios Epidemiológicos (CES-D) en población indígena mexicana.

    PubMed

    Franco-Díaz, Karen Lizbeth; Fernández-Niño, Julián Alfredo; Astudillo-García, Claudia Iveth

    2018-05-01

    Introducción. La versión breve de la Escala de Depresión del Centro de Estudios Epidemiológicos (CESD) es un recurso factible para la tamización de los síntomas de depresión en la población general, pero no se ha reportado la prevalencia en la población indígena, ni su invarianza factorial en Latinoamérica.Objetivo. Describir la prevalencia de los síntomas de depresión y la invarianza factorial de la versión breve de la escala CES-D en población indígena mexicana.Materiales y métodos. Se hizo un estudio transversal en una muestra representativa de 37.165 adultos mexicanos de 20 a 59 años de edad. La identidad indígena se determinó mediante el propio reporte de la persona como hablante de una lengua indígena. Se conformaron ocho grupos de análisis según el sexo, el alfabetismo y el ser indígena. Se describió la prevalencia de los síntomas depresivos en cada grupo, así como la invarianza factorial de la configuración de los perfiles mediante un análisis factorial exploratorio. Las matrices de varianza y covarianza se compararon entre pares de perfiles usando el test modificado de Mantel.Resultados. La prevalencia de síntomas depresivos en mujeres indígenas que sabían leer fue de 16,8 % (IC95%: 13,4-20,3); en mujeres indígenas que no sabían leer, de 21,3 % (IC95%: 15,5-27,1); en hombres indígenas que sabían leer de 8,5 % (IC95%: 6,0-11,1), y en hombres indígenas que no sabían leer de 10,4 % (IC95%: 5,2-15,6). No se encontraron diferencias significativas en las cargas factoriales entre los perfiles.Conclusión. Se reportó una menor prevalencia de síntomas depresivos en indígenas que en la población no indígena. La escala CES-D en su versión breve mostró invarianza factorial al emplearla en la población indígena.

  11. Combined experimental and numerical approach to evaluate impact scaling relations and reproducibility of craters produced at the Experimental Projectile Impact Chamber (E.P.I.C., Centro de Astrobiología, Spain.)

    NASA Astrophysics Data System (ADS)

    Ormö, J.; Wünnemann, K.; Collins, G.; Melero Asensio, I.

    2012-04-01

    The Experimental Projectile Impact Chamber at Centro de Astrobiología, Spain, consists of a 7m wide, funnel-shaped test bed, and a 20.5mm caliber compressed N2 gas gun. The test bed can be filled with any type of target material, but is especially designed for wet target experiments. The shape and size aim to decrease disturbance from reflected surface waves in wet target experiments. Experiments are done under 1Atm pressure. The gas gun can launch projectiles of any material and dimensions <20mm (smaller diameters using sabots), and at any angle from vertical to near horizontal. The projectile velocities are of the order of a few hundreds of meters per second depending mainly on the gas pressure, as well as projectile diameter and density. When using a dry sand target a transient crater about 30cm wide is produced. Wet target experiments have not yet been performed in this newly installed test chamber, but transient cavities in water are expected to be in the order of 50-70cm wide. The large scale allows for detailed study of the dynamics of cratering motions during the stages of crater growth and subsequent collapse, especially in wet targets. These observations provide valuable benchmark data for numerical simulations and for comparison with field studies. Here we describe the results of ten impact experiments using three different gas pressures (100bar, 180bar, 200bar), two projectile compositions (20mm, 5.7g delrin; 20mm, 16.3g Al2O3), and two different impact angles (90˚ and 53˚ over the horizontal plane). Nine of the experiments were done in a quarter-space geometry using a specially designed camera tank with a 45mm thick glass window. One experiment was done in half-space geometry as reference. The experiments were recorded with a high-speed digital video camera, and the resulting craters were documented with a digital still frame camera. Projectile velocities are estimated with a combination of tracking software and a Shooting Chrony Alpha M-1

  12. [Comparing the Application of Hema-Obs RSS to 250 Pregnancies from Obstetrics/Hematology Consultation in Centro Hospitalar São João, Portugal with the Application of Galit Sarig RSS to 90 Pregnancies from Rambam Health Care Campus, Israel].

    PubMed

    Salselas, Ana; Pestana, Inês; Bischoff, Francisco; Guimarães, Mariana; Andrade, Joaquim Aguiar

    2015-01-01

    Pregnant women with thromboembolic diseases, previous thrombotic episodes or thrombophilia family history were supervised in a multidisciplinary Obstetrics/ Hematology consultation in Centro Hospitalar São João EPE, Porto, Portugal. For the evaluation and medication of these women, a risk stratification scale was used. The aim of this study was to validate a Risk Stratification Scale and thromboprophylaxis protocol by means of comparing it with a similar scale, developed and published by Sarig. We have compared: The distribution, by risk groups, obtained through the application of the two scales on pregnant women followed at Centro Hospitalar São João, Porto, Portugal, consultation; the sensibility and specificity for each one of the scales (DeLong scale, applied to Receiver Operating Characteristic) curves; the outcomes in pregnancies followed in Hospital São João, Porto, PortugalResults: According to our Hema-Obs risk stratification scale, 29% were allocated to low-risk, 47% to high-risk and 24% to very-high-risk groups. According to Galit Sarig risk stratification scale, 24% were considered low-risk, 53% moderate, 16% high-risk and 7% as very high-risk group. In our study we observed 9% of spontaneous abortions, in comparison with 18% in the Galit Sarig cohort. From the application of Receiver Operating Characteristic curve to both risk stratification scales, the results of the calculated areas were 58,8% to our Hema-Obs risk stratification scale and 38,7% to Galit Sarig risk stratification scale, with a Delong test significancie of p = 0.0006. We concluded that Hema-Obs risk stratification scale is an effective support for clinical monitoring of therapeutic strategies.

  13. Language as Cultural Practice: Mexicanos en el Norte.

    ERIC Educational Resources Information Center

    Schecter, Sandra R.; Bayley, Robert

    This book offers an ethnographic account of language socialization practices within Mexican-background families residing in California and Texas. It illustrates cases where language is used by speakers to choose between alternative self-definitions and where language interacts differentially with other defining categories (ethnicity, gender, and…

  14. 76 FR 13124 - Del Norte Resource Advisory Committee (RAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-10

    ...: Adam Dellinger, Committee Coordinator, Six Rivers National Forest, at (707) 441-3569; e-mail adellinger... recommended projects and will vote on projects to recommend for funding. The meeting is open to the public and...

  15. 75 FR 78966 - Del Norte Resource Advisory Committee (RAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-17

    ... Washington Boulevard, Crescent City, California 95531. FOR FURTHER INFORMATION CONTACT: Adam Dellinger..., the public will present clarification on previously submitted Title II project proposals to the RAC. The RAC will vote on projects to recommend for funding. There will also be a public comment...

  16. 76 FR 8333 - Del Norte Resource Advisory Committee (RAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-14

    ..., 301 West Washington Boulevard, Crescent City, California 95531. FOR FURTHER INFORMATION CONTACT: Adam... solicitations and will vote on projects to recommend for funding. The meeting is open to the public and there...

  17. Tectonica activa y geodinamica en el norte de centroamerica

    NASA Astrophysics Data System (ADS)

    Alvarez Gomez, Jose Antonio

    The rampant success of quantum theory is the result of applications of the 'new' quantum mechanics of Schrodinger and Heisenberg (1926-7), the Feynman-Schwinger-Tomonaga Quantum Electro-dynamics (1946-51), the electro-weak theory of Salaam, Weinberg, and Glashow (1967-9), and Quantum Chromodynamics (1973-); in fact, this success of 'the' quantum theory has depended on a continuous stream of brilliant and quite disparate mathematical formulations. In this carefully concealed ferment there lie plenty of unresolved difficulties, simply because in churning out fabulously accurate calculational tools there has been no sensible explanation of all that is going on. It is even argued that such an understanding is nothing to do with physics. A long-standing and famous illustration of this is the paradoxical thought-experiment of Einstein, Podolsky and Rosen (1935). Fundamental to all quantum theories, and also their paradoxes, is the location of sub-microscopic objects; or, rather, that the specification of such a location is fraught with mathematical inconsistency. This project encompasses a detailed, critical survey of the tangled history of Position within quantum theories. The first step is to show that, contrary to appearances, canonical quantum mechanics has only a vague notion of locality. After analysing a number of previous attempts at a 'relativistic quantum mechanics', two lines of thought are considered in detail. The first is the work of Wan and students, which is shown to be no real improvement on the iisu.al 'nonrelativistic' theory. The second is based on an idea of Dirac's - using backwards-in-time light-cones as the hypersurface in space-time. There remain considerable difficulties in the way of producing a consistent scheme here. To keep things nicely stirred up, the author then proposes his own approach - an adaptation of Feynman's QED propagators. This new approach is distinguished from Feynman's since the propagator or Green's function is not obtained by Feynman's rule. The type of equation solved is also different: instead of an initial-value problem, a solution that obeys a time-symmetric causality criterion is found for an inhomogeneous partial differential equation with homogeneous boundary conditions. To make the consideration of locality more precise, some results of Fourier transform theory are presented in a form that is directly applicable. Somewhat away from the main thrust of the thesis, there is also an attempt to explain, the manner in which quantum effects disappear as the number of particles increases in such things as experimental realisations of the EPR and de Broglie thought experiments.

  18. Rio Grande del Norte National Conservation Area Establishment Act

    THOMAS, 111th Congress

    Sen. Bingaman, Jeff [D-NM

    2009-04-23

    Senate - 03/02/2010 Placed on Senate Legislative Calendar under General Orders. Calendar No. 285. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  19. Rio Grande del Norte National Conservation Area Establishment Act

    THOMAS, 112th Congress

    Sen. Bingaman, Jeff [D-NM

    2011-03-29

    Senate - 01/13/2012 Placed on Senate Legislative Calendar under General Orders. Calendar No. 275. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  20. 75 FR 11104 - Del Norte Resource Advisory Committee (RAC)

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-10

    ... Schools and Community Self-Determination Act (Pub. L. 110-343) and in compliance with the Federal Advisory...; (4) discussion of Committee member and Designated Federal Official roles and (5) review operational...

  1. 78 FR 50025 - Del Norte County Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-16

    ... authorized under the Secure Rural Schools and Community Self-Determination Act (Act) (Pub. L. 112-141) and... Service concerning projects and funding consistent with the Title II of the Act. The meetings are open to the public. The purpose of the meetings are to review prior year project's progress and to review and...

  2. 77 FR 47360 - Del Norte Resource Advisory Committee

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-08

    ... Community Self-Determination Act (Pub. L. 112-141) (the Act) and operates in compliance with the Federal... provide advice and recommendations to the Forest Service concerning projects and funding consistent with... 2011 project status and to discuss process the Committee will use to review and recommend fiscal year...

  3. PIA Program | CTIO

    Science.gov Websites

    se cuenta con los fondos necesarios para efectuar el Programa PIA para el 2018. We do, on occasion Observatorio Inter-Americano de Cerro Tololo es el centro nacional de los Estados Unidos para el desarrollo de unos 400 kms al norte de Santiago. Los telescopios se ubican en la cima de Cerro Tololo, a 90 kms al

  4. Servicos Do Centro De Solucoes De Energia Limpa (Fact Sheet)

    SciTech Connect

    Not Available

    2014-06-01

    This is the Portuguese translation of the Clean Energy Solutions Center Services fact sheet. The Solutions Center helps governments, advisors and analysts create policies and programs that advance the deployment of clean energy technologies. The Solutions Center partners with international organizations to provide online training, expert assistance, and technical resources on clean energy policy.

  5. CNEA - Centro Atómico Bariloche

    Science.gov Websites

    ³mica (CNEA), a través de la Sección de... EL RA-6, RENOVADO EN EL AÑO DE SU 35º ANIVERSARIO EL RA-6 DIRIGIDAS A DOCENTES REALIZAN EN EL CAB CAPACITACIONES DIRIGIDAS A... La Comisión Nacional de Energía Atà ... RECONOCIMIENTO PARA LA SECCIÓN DE DIVULGACIÓN CIENTÍFICA Y TECNOLÓGICA DEL CAB RECONOCIMIENTO PARA LA SECCIÃ

  6. Papers Selected for Presentation at the International Symposium on Remote Sensing of Environment (16th) Held at Buenos Aires, Argentina on 2-9 June 1982. Volume 2.

    DTIC Science & Technology

    1982-06-01

    ARGENTINA Oscar Dominguez and Stella Carballo .............. 355 STUDIES ON SOME URBAN PROBLEMS USING AIRBORNE REMOTE SENSORS IN SANTIAGO, CHILE ...SAN JUAN ARGENTINA) (1) Silvia Lendaro de Gianni (2,3) Enrique Uliarte (I) Centro Regional de Agua Subterrgnea (2) Universidad Nacional de San Juan act...Hidrogeol6gico para refuerzo do la provisi6n de agua " a Puerto Deseado. In4dito. - Pezzuchi, Hugo Daniel (1978) "studio Geol6gico de la zona de Lstancia Dos

  7. Teledermatology in Tijuana, Mexico.

    PubMed

    Brown, Megan

    2016-12-01

    The Health Frontiers in Tijuana (HFiT) clinic is a binational partnership between the University of California, San Diego School of Medicine (San Diego, California); the Universidad Autónoma de Baja California School of Medicine (Tijuana, Mexico); and Desayunador Salesiano Padre Chava, a community grassroots organization in Tijuana, Mexico. Health Frontiers in Tijuana provides accessible quality health care for the underserved in Tijuana's Zona Norte. This article is a narrative meant to share my clinical experience as a dermatology resident who worked with HFiT to establish teledermatology services at this clinic.

  8. Pathophysiology of white-tailed deer vaccinated with porcine zona pellucida immunocontraceptive

    USGS Publications Warehouse

    Curtis, P.D.; Richmond, M.E.; Miller, L.A.; Quimby, F.W.

    2007-01-01

    White-tailed deer (n = 14 treated, n = 7 control) were examined postmortem to identify any possible pathophysiology resulting from PZP immunocontraception vaccination. Deer were treated twice in 1997; given a booster in 1998, with six being revaccinated in September 2000. Granulomas were found at injection sites of most deer, even 2 years post-treatment. Eosinophilic oophoritis occurred in 6 of 8 (75%) deer vaccinated in 1998, and 3 of 6 (50%) revaccinated in 2000. The 2000 revaccinates without oophoritis, had significantly fewer normal secondary follicles than control females (P = 0.03), and deer in the1998 treatment group (P = 0.04). PZP immunocontraceptive vaccine elicited ovarian pathologies in deer similar to those observed in other species. ?? 2007 Elsevier Ltd. All rights reserved.

  9. Age-related effects of dexamethasone administration in adrenal zona reticularis.

    PubMed

    Almeida, Henrique; Matos, Liliana; Ferreira, Jorge; Neves, Delminda

    2006-05-01

    Suppression of adrenocorticotropic hormone results in reduced adrenal steroid output, adrenocortical cell atrophy, and apoptosis in young rats. To verify such effects during aging, dexamethasone was injected into rats for 3 days at five different ages; at day 4, adrenals and blood were collected for morphologic and corticosterone assay. Adrenal structure was similar at all ages, but in dexamethasone-injected animals there were ultrastructural features of apoptosis and a higher percentage of TUNEL and caspase-3-labeled nuclei and cytoplasm; their corticosterone decreased significantly. In both groups, there was age-related decrease in the percentage of apoptotic cells, significant only in dexamethasone-injected rats. The data suggest that aged adrenocortical cells are less susceptible to the lack of adrenocorticotropic hormone (ACTH), possibly as a result of their decreased functional ability.

  10. Identification of ZASP, a novel protein associated to Zona occludens-2.

    PubMed

    Lechuga, Susana; Alarcón, Lourdes; Solano, Jesús; Huerta, Miriam; Lopez-Bayghen, Esther; González-Mariscal, Lorenza

    2010-11-15

    With the aim of discovering new molecular interactions of the tight junction protein ZO-2, a two-hybrid screen was performed on a human kidney cDNA library using as bait the middle segment of ZO-2. Through this assay we identified a 24-kDa novel protein herein named ZASP for ZO-2 associated speckle protein. ZO-2/ZASP interaction further confirmed by pull down and immunoprecipitation experiments, requires the presence of the intact PDZ binding motif SQV of ZASP and the third PDZ domain of ZO-2. ZASP mRNA and protein are present in the kidney and in several epithelial cell lines. Endogenous ZASP is expressed primarily in nuclear speckles in co-localization with splicing factor SC-35. Nocodazole treatment and wash out reveals that ZASP disappears from the nucleus during mitosis in accordance with speckle disassembly during metaphase. ZASP amino acid sequence exhibits a canonical nuclear exportation signal and in agreement the protein exits the nucleus through a process mediated by exportin/CRM1. ZASP over-expression blocks the inhibitory activity of ZO-2 on cyclin D1 gene transcription and protein expression. The identification of ZASP helps to unfold the complex nuclear molecular arrays that form on ZO-2 scaffolds. Copyright © 2010 Elsevier Inc. All rights reserved.

  11. Epigallocatechin-3-Gallate (EGCG) Reduces Rotenone Effect on Stallion Sperm-Zona Pellucida Heterologous Binding.

    PubMed

    Plaza Dávila, M; Bucci, D; Galeati, G; Peña, F J; Mari, G; Giaretta, E; Tamanini, C; Spinaci, M

    2015-12-01

    Stallion spermatozoa are highly dependent on oxidative phosphorylation for ATP production to achieve normal sperm function and to fuel the motility. The aim of this study was to evaluate the response of equine sperm under capacitating conditions to the inhibition of mitochondrial complex I by rotenone and to test whether epigallocatechin-3-gallate (EGCG), a natural polyphenol component of green tea, could counteract this effect. After 2-h incubation of stallion spermatozoa in modified Tyrode's medium, rotenone (100 nm, 500 nm and 5 μm) and EGCG (10, 20 and 60 μm), alone or in combination, did not induce any significant difference on the percentage of viable cells, live sperm with active mitochondria and spermatozoa with intact acrosome. The inhibition of complex I of mitochondrial respiratory chain of stallion sperm with rotenone exerted a negative effect on heterologous ZP binding ability. EGCG at the concentrations of 10 and 20 μm (but not of 60 μm) induced a significant increase in the number of sperm bound to the ZP compared with that for control. Moreover, when stallion sperm were treated with rotenone 100 nm, the presence of EGCG at all the concentrations tested (10, 20 and 60 μm) significantly increased the number of sperm bound to the ZP up to control levels, suggesting that this green tea polyphenol is able to reduce the toxicity of rotenone. © 2015 Blackwell Verlag GmbH.

  12. "VAMONOS PAL NORTE" (LET'S GO NORTH), A SOCIAL PROFILE OF THE SPANISH SPEAKING MIGRATORY FARM LABORER.

    ERIC Educational Resources Information Center

    A YEARLY INCREASE OF MIGRATORY WORKERS TO OREGON RESULTED IN HIGHER WAGES IN THE AREA. IN 1957 THE SPANISH SPEAKING LABORERS IN OREGON NUMBERED 11,000 TO 12,000, 10 PERCENT OF WHOM WERE PERMANENT RESIDENTS. RECRUITMENT WAS CARRIED OUT BY CONTRACTORS. THEY OPERATED THROUGH SUBCONTRACTORS WHO IN TURN ACTED AS CONTACT MEN IN STRATEGIC LOCATIONS…

  13. 78 FR 18783 - Establishment of the R[iacute]o Grande del Norte National Monument

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-28

    ... the landscape by the remnants of homes, root cellars, cistern-style water catchments, and cast metal... management of the objects to be protected; WHEREAS it is in the public interest to preserve the objects of... approximately 242,555 acres, which is the smallest area compatible with the proper care and management of the...

  14. Pathways to El Norte: Origins, Destinations, and Characteristics of Mexican Migrants to the United States

    PubMed Central

    Riosmena, Fernando; Massey, Douglas S.

    2013-01-01

    The geography Mexican migration to the U.S. has experienced deep transformations in both its origin composition and the destinations chosen by migrants. To date, however, we know little about how shifting migrant origins and destinations may be linked to one another geographically and, ultimately, structurally as relatively similar brands of economic restructuring have been posited to drive the shifts in origins and destinations. In this paper, we describe how old and new migrant networks have combined to fuel the well-documented geographic expansion of Mexican migration. We use data from the 2006 Mexican National Survey of Population Dynamics, a nationally representative survey that for the first time collected information on U.S. state of destination for all household members who had been to the United States during the five years prior to the survey. We find that the growth in immigration to southern and eastern states is disproportionately fueled by undocumented migration from non-traditional origin regions located in Central and Southeastern Mexico and from rural areas in particular. We argue that economic restructuring in the U.S. and Mexico had profound consequences not only for the magnitude but also for the geography of Mexican migration, opening up new region-to-region flows. PMID:22666876

  15. Border Environmental Education Resource Guide: Southern New Mexico, South Texas, Northern Chihuahua, Northern Coahuila, Northern Nuevo Leon, Northern Tamaulipas = Guia de Recursos de Educacion Ambiental en la Frontera: Sur de Nuevo Mexico, Sur de Texas, Norte de Chihuahua, Norte de Coahuila, Norte de Nuevo Leon, Norte de Tamaulipas.

    ERIC Educational Resources Information Center

    Meissner, David, Comp.

    This guide provides educators and residents of the border with useful information about environmental education program offerings along the eastern half of the United States-Mexico border. The programs listed in the guide represent a broad range of educational efforts focused on understanding the environment and solving environmental problems in…

  16. Pathways to El Norte: origins, destinations, and characteristics of Mexican migrants to the United States.

    PubMed

    Riosmena, Fernando; Massey, Douglas S

    2012-01-01

    The geography Mexican migration to the U.S. has experienced deep transformations in both its origin composition and the destinations chosen by migrants. To date, however, we know little about how shifting migrant origins and destinations may be linked to each another geographically and, ultimately, structurally as relatively similar brands of economic restructuring have been posited to drive the shifts in origins and destinations. In this paper, we describe how old and new migrant networks have combined to fuel the well-documented geographic expansion of Mexican migration. We use data from the 2006 Mexican National Survey of Population Dynamics, a nationally representative survey that for the first time collected information on U.S. state of destination for all household members who had been to the U.S. during the 5 years prior to the survey. We find that the growth in immigration to southern and eastern states is disproportionately fueled by undocumented migration from non-traditional origin regions located in Central and Southeastern Mexico and from rural areas in particular. We argue that economic restructuring in the U.S. and Mexico had profound consequences not only for the magnitude but also for the geography of Mexican migration, opening up new region-to-region flows.

  17. Historia Verdadera del Chicano del Norte. [True History of the Chicano of the North].

    ERIC Educational Resources Information Center

    Herrera, David; Bryan, Clifford E.

    Very little is known about the Chicanos in the northern U.S., especially those in the Northwest. Research and writing on Chicanos have concentrated on the Southwest or large urban settings, thus excluding those Chicanos residing in rural settings except for those in the migrant stream. These rural residents have become a forgotten people,…

  18. Diversidad de aves en agropaisajes en la region norte de Nicaragua

    Treesearch

    Wayne J. Arendt; Marvin Tórrez; Sergio Vílchez

    2012-01-01

    Avian diversity in agroscapes in Nicaragua’s north highlands. – Nicaragua’s highland forests are threatened by continual wood extraction and encroaching agriculture. Still, the effects of forest loss and fragmentation on avian communities remain little known. We used fixed-width point counts (distance sampling: 4843 detections during 86 h of observation) to...

  19. Parental Strategies in Contrasting Cultural Settings: Families in Mexico and "El Norte."

    ERIC Educational Resources Information Center

    Reese, Leslie

    2002-01-01

    Compared the child-rearing practices and values of low-income Mexican immigrants raising their children in the United States with those of their siblings raising children in Mexico, discussing family accommodation within the ecocultural niche, variation in ecocultural contexts, effects of immigration, cultural change of different types, and…

  20. Carbon isotope geochemistry of hydrocarbons in the Cerro Prieto geothermal field, Baja California Norte, Mexico

    NASA Technical Reports Server (NTRS)

    Des Marais, D. J.; Stallard, M. L.; Nehring, N. L.; Truesdell, A. H.

    1988-01-01

    Hydrocarbon abundances and stable-isotopic compositions were measured in wells M5, M26, M35 and M102, which represent a range of depths (1270-2000 m) and temperatures (275-330 degrees C) in the field. In order to simulate the production of the geothermal hydrocarbons, gases were collected from the pyrolysis of lignite in the laboratory. This lignite was obtained from a well which sampled rock strata which are identical to those occurring in the field, but which have experienced much lower subsurface temperatures. In both the well and the laboratory observations, high-temperature environments favored higher relative concentrations of methane, ethane and benzene and generally higher delta 13C-values in the individual hydrocarbons. The best correlation between the laboratory and well data is obtained when laboratory-produced gases from experiments conducted at lower (400 degrees C) and higher (600 degrees C) temperatures are mixed. This improved correlation suggests that the wells are sampling hydrocarbons produced from a spectrum of depths and temperatures in the sediments.

  1. Mineral resources of the southern half of Zone III Santander, Norte de Santander and Boyaca, Colombia

    USGS Publications Warehouse

    Ward, Dwight Edward; Goldsmith, Richard; Cruz, Bruna B.; Restrepo, Jaime; Hernan, A.

    1970-01-01

    The areas covered by this report lies in the eastern Cordillera of the Colombian Andes in the region around Bucaramanga. This part of the eastern Cordillera consists of a structurally complex core of metamorphic and igneous rocks of Precambrian to Mesozoic age, flanked to east and west by faulted and folded sedimentary strata of late Paleozoic to Tertiary age. Infaulted blocks of sedimentary rocks are locally present in the massif. Unconsolidated deposits of Quaternary age, primarily terraced alluvium, are 10cally extensive in valleys on the flanks of the range. The crystalline central core of the range is called the Santander massif. In it are located the principal sold deposits and scattered deposits of copper, lead, zinc, and fluorite. The sedimentary rocks flanking the massif contain significant deposits of phosphate rock and gypsum, as well as other nonmetallic industrial minerals such as limestone, barite, glass sand, and coal. A belt of lead-zinc prospects in carbonate and sandstone beds of Cretaceous age on the east side of the range warrants further investigation. Gold and silver are the only important metallic minerals that have been produced in the Santander massif. Mining dates back to colonial and possibly to pre-colonial times and continues on a small scale at present. The California and Vetas district was the main area of investigation of metallic minerals during the present project. Results of geochemical sampling of stream sediments and assays of vein material indicate that the main potential of the area is in gold with lesser potentials in copper, lead, zinc, and silver. Mineralization of the district is probably younger than Early Cretaceous. Although no copper minerals have been mined elsewhere in the massif, small amounts of copper minerals in various rocks in scattered areas is revealed by green and blue stains of copper carbonates and sulfates. Deposits of greatest areal extent are in arkosic conglomeratic beds of the Giron Formation. These are being explored and sampled at the present time (1969). A little lead has been mined and smelted in the past but operations were on a very small scale and of short duration. Small amounts of lead, zinc, and copper minerals accompany dolomite replacement of Cretaceous limestone in a few scattered places, and several promising prospects are being investigated by means of trenches and drilling. One magnetite and several hematite prospects were examined but none offers any potential for economic development. Thick beds of gypsum in Lower Cretaceous limestone on Mesa de Los Santos, south of Bucaramanga are being quarried from outcrops for use in cement manufacture. The deposit was discovered shortly before the present project began, and although its extent beneath overlying strata is not yet determined by drilling, it appears to be in a small evaporite basin of about three kilometers in radius. Reserves of gypsum are large, but future development will have to be by underground mining. Outcrops of Cretaceous limestone of high purity are widespread and are more than adequate to meet all demands, which at present are for cement and calcined lime, road construction material, and to a small extent for agricultural lime and polished decorative stone. Upper Paleozoic limestone of the Diamante Formation crops out in a few places; it has been used near Bucaramanga for cement manufacture. Marble is present in several localities of the Santander massif in Lower Paleozoic and Devonian rocks. Impurities, fractures, and solution cavities render most of it unsuitable for decorative purposes, but selected parts are used in floor tile and terrazo. Recrystallized limestone of the Diamante Formation in the same area, usually referred to as marble, is of uniform high purity throughout a thick and uninterrupted section, and offers a good source of limestone raw material. A little is now used for agricultural lime. The potential of this resource has not been fully evalua

  2. Evaluating outcome-correlated recruitment and geographic recruitment bias in a respondent-driven sample of people who inject drugs in Tijuana, Mexico

    PubMed Central

    Rudolph, Abby E.; Gaines, Tommi L.; Lozada, Remedios; Vera, Alicia; Brouwer, Kimberly C.

    2015-01-01

    Respondent-driven sampling’s (RDS) widespread use and reliance on untested assumptions suggests a need for new exploratory/diagnostic tests. We assessed geographic recruitment bias and outcome-correlated recruitment among 1048 RDS-recruited people who inject drugs (Tijuana, Mexico). Surveys gathered demographics, drug/sex behaviors, activity locations, and recruiter-recruit pairs. Simulations assessed geographic and network clustering of active syphilis (RPR titers≥1:8). Gender-specific predicted probabilities were estimated using logistic regression with GEE and robust standard errors. Active syphilis prevalence was 7% (crude: men=5.7% and women=16.6%; RDS-adjusted: men=6.7% and women=7.6%). Syphilis clustered in the Zona Norte, a neighborhood known for drug and sex markets. Network simulations revealed geographic recruitment bias and non-random recruitment by syphilis status. Gender-specific prevalence estimates accounting for clustering were highest among those living/working/injecting/buying drugs in the Zona Norte and directly/indirectly connected to syphilis cases (men:15.9%, women:25.6%) and lowest among those with neither exposure (men:3.0%, women:6.1%). Future RDS analyses should assess/account for network and spatial dependencies. PMID:24969586

  3. Fotometría superficial de la galaxia irregular NGC 1427A

    NASA Astrophysics Data System (ADS)

    Cellone, S. A.; Forte, J. C.

    Se presenta fotometría superficial multicolor de la galaxia irregular NGC 1427A. Esta galaxia, un miembro del Cúmulo de Fornax que no ha sido estudiado previamente, muestra una morfología muy particular: una distribución estelar subyacente de bajo brillo superficial (LSB) con varias manchas brillantes dispuestas en una estructura anular deformada. Por sus colores muy azules se infiere que estas son zonas de formación estelar reciente. Al norte del cuerpo principal de la galaxia, y conectado con éste mediante filamentos LSB, hay un objeto de isofotas aproximadamente circulares. Este objeto tiene en sí mismo algunas zonas brillantes, pero su población estelar subyacente es significativamente más azul que la del cuerpo principal de la galaxia. Esto indica que ambos objetos han tenido distintas historias evolutivas. Estructuralmente, el objeto del norte es muy similar a algunas de las galaxias elípticas enanas más débiles que pueblan el Cúmulo de Fornax. Se discute la posible relación entre este objeto y la galaxia en si.

  4. Evaluating outcome-correlated recruitment and geographic recruitment bias in a respondent-driven sample of people who inject drugs in Tijuana, Mexico.

    PubMed

    Rudolph, Abby E; Gaines, Tommi L; Lozada, Remedios; Vera, Alicia; Brouwer, Kimberly C

    2014-12-01

    Respondent-driven sampling's (RDS) widespread use and reliance on untested assumptions suggests a need for new exploratory/diagnostic tests. We assessed geographic recruitment bias and outcome-correlated recruitment among 1,048 RDS-recruited people who inject drugs (Tijuana, Mexico). Surveys gathered demographics, drug/sex behaviors, activity locations, and recruiter-recruit pairs. Simulations assessed geographic and network clustering of active syphilis (RPR titers ≥1:8). Gender-specific predicted probabilities were estimated using logistic regression with GEE and robust standard errors. Active syphilis prevalence was 7 % (crude: men = 5.7 % and women = 16.6 %; RDS-adjusted: men = 6.7 % and women = 7.6 %). Syphilis clustered in the Zona Norte, a neighborhood known for drug and sex markets. Network simulations revealed geographic recruitment bias and non-random recruitment by syphilis status. Gender-specific prevalence estimates accounting for clustering were highest among those living/working/injecting/buying drugs in the Zona Norte and directly/indirectly connected to syphilis cases (men: 15.9 %, women: 25.6 %) and lowest among those with neither exposure (men: 3.0 %, women: 6.1 %). Future RDS analyses should assess/account for network and spatial dependencies.

  5. The Role of Sex and Mating Status in the Expansion Process of Arhopalus rusticus (Coleoptera: Cerambycidae)-an Exotic Cerambycid in Argentina.

    PubMed

    Grilli, Mariano Pablo; Fachinetti, Romina

    2017-06-01

    ón potencial de esta especie. Utilizamos individuos recolectados de los bosques de pino implantados en la región centro oeste de la provincia de Córdoba (Argentina). La capacidad de dispersión de Arhopalus rusticus se determinó haciendo volar individuos recién emergidos en un molino de vuelo. Se registró el peso previo y posterior al vuelo, el largo del cuerpo y el tamaño del élitro. Las hembras fueron mejores voladoras, más largas, más pesadas, con élitros más largos que los machos. Observamos diferencias entre el potencial de dispersión dependiendo del estado de apareamiento de los individuos. Los individuos apareados volaron más rápido y mayores distancias que los individuos no apareados y consumieron una menor fracción de su peso corporal. Se observó una relación positiva entre el tamaño del élitro y la velocidad de vuelo. Se generó un mapa de las distancias de dispersión potencial de los machos y de las hembras para la región de estudio utilizando las distribuciones de las distancias obtenidas del molino de vuelo. La dispersión post copulación incrementa las chances de que la especie se establezca en zonas no ocupadas. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  6. Suitability Measurement and Analysis for El Centro Naval Air Facility OLS. Opportune Landing Site Program

    DTIC Science & Technology

    2008-10-01

    western corner and at a few other sample locations. For most of the RAS the surface layer CBR values are in the teens , with a few less than 10 and...from vandalism . Carl offered that we can tie in with weather station near the Range Control, Building 5000, and weather station in Target Area 103. We...to some degree. We noted that if we were to select this OLS, the instrumenta- tion may be destroyed or vandalized . This was a potential site and

  7. Energia Renovable para Centros de Salud Rurales (Renewable Energy for Rural Health Clinics) (in Spanish)

    SciTech Connect

    Jimenez, T.; Olson, K.

    Esta es la primera de una serie de guias de aplicaciones que el Programa de Energia de Villas de NREL esta comisionando para acoplar sistemas comerciales renovables con aplicaciones rurales, incluyendo agua, escuelas rurales y micro empresas. La guia esta complementada por las actividades de desarrollo del Programa de Energia de Villas de NREL, proyectos pilotos internacionales y programas de visitas profesionales.

  8. Servicios del Centro de Soluciones Para la Energia Limpia (Fact Sheet)

    SciTech Connect

    Not Available

    2014-05-01

    This is the Spanish translation of the Clean Energy Solutions Center Services fact sheet. The Clean Energy Solutions Center (Solutions Center) helps governments, advisors and analysts create policies and programs that advance the deployment of clean energy technologies. The Solutions Center partners with international organizations to provide online training, expert assistance, and technical resources on clean energy policy.

  9. La Communicacion entre los Centros de Investigacion en Educacion (Communication among Educational Research Centers).

    ERIC Educational Resources Information Center

    Schiefelbein, Ernesto

    1972-01-01

    In Latin America there is a lack of communication concerning educational research. This lack has been underlined in many regional meetings, but no action has been taken. Possible steps that would lead to improvement include circulation of research summaries, both for completed and current works, efforts by research centers to organize meetings,…

  10. Centro de Biologia Molecular "Severo Ochoa": a center for basic research into Alzheimer's disease.

    PubMed

    Avila, Jesus; Hernandez, Felix; Wandosell, Francisco; Lucas, Jose J; Esteban, Jose A; Ledesma, M Dolores; Bullido, Maria J

    2010-01-01

    One important aspect of studies carried out at the Center for Molecular Biology "Severo Ochoa" is focused on basic aspects of Alzheimer's disease, mainly the search for suitable therapeutic targets for this disorder. Several groups at the Center are involved in these studies, and, in this spotlight, the work they are carrying out will be described.

  11. Summary Report on Visit to Departamento de Investigation Centro Ramon Y Cajal Madrid, Spain.

    DTIC Science & Technology

    1984-10-30

    Over one hundred colonies of Gram negative bacteria ( Lactobacillus acidophilus ) were cultured at 380 C in Petri dishes for 72 hours. The inoculum...within the various laboratories. He was particularly excited about studies using Lactobacillus bacteria and the work involving Drosophila melanogaster...therapy (0-40 Gauss) caUsed a 40 %reduction in the number of Lactobacillus colonies. ........................ . . .. . .. . .. . .. . . RAT MODEL TO

  12. [Rural women. Public health workshop of the Centro de Investigaciones en Psicologia Social].

    PubMed

    Juarez Bartola, M; Garcia Ventura, H; De Jesus Leon, M J

    1993-04-01

    Mexico's peasant sector is undergoing great change as a result of the insertion of capitalist relations into areas that were previously remote. The traditional peasant subsistence economy has been displaced, and peasants have been forced to adopt a series of new strategies for survival, entailing proletarianization or selling of labor. Women in the community of San Nicolas Zoyapetlayoca, in the municipio of Tepeaca, have lived in a situation of poverty and precariousness for some time. Most of the population historically depended on the neighboring haciendas for their livelihood. Women and children also worked there, mistreated and without social protection. The lands that were distributed after the Revolution are insufficient to support the community, and most families must look elsewhere for employment. Incomes are insufficient to cover all the necessities of education, health, nutrition, and other aspects of life. Most women are educated no farther than the primary level. They spend most of their time preparing food, carrying water, washing clothes, caring for their children, and in other domestic chores. Some women work for wages as laborers, factory workers, or domestics. Rural women are disadvantaged by poor working conditions, insufficient education, low levels of health and nutrition, limited participation in social and political life, poor housing and lack of services, and frequently by their own attitudes of traditionalism or fatalism.

  13. [Family. Public health workshop of the Centro de Investigaciones en Psicologia Social].

    PubMed

    Sanchez Maria Felix, B; Leon, M J

    1993-04-01

    An abbreviated primer on the sociology of the family is presented. The work defines family, describes different types of family structure, outlines the family life cycle, and discusses the functioning of families. Human beings in all societies require membership in a family of some type for reproduction and socialization. One of the most basic human needs is for a mother who will feed, protect, and instruct her offspring. Families have always changed in response to societal changes. The functions of a family are devoted to two different sets of objectives, the internal ones of psychosocial protection of the members and the external ones of accommodation to and transmission of a culture. Families in all cultures provide their members with a sense of identity. Families must respond to internal and external changes and should be capable of transforming themselves to adjust to new circumstances without losing continuity. Family systems differentiate themselves to carry out their functions through their subsystems. Individuals and dyads such as maternal-child or husband-wife are the subsystems. The family life cycle entails a series of stages: courtship, marriage, the birth and growth of children, separation of the children, retirement, old age. Each stage has its own pitfalls and challenges. Each family has a hierarchy of status and power, although in some cases the proper hierarchies are inverted, and the children carry out functions that should be completed by the parents. A series of explicit and implicit, rigid and flexible rules guide the functioning of the family. Various roles are carried out, including parental, marital, and sibling roles, as well as roles of authority, administration, and messenger.

  14. Servicios del Centro de Soluciones Para la Energia Limpia (Spanish Translation)

    SciTech Connect

    This is a Spanish translation of the Clean Energy Solutions Center fact sheet. The Solutions Center offers no-cost expert policy assistance, webinars and training forums, clean energy policy reports, data, and tools provided in partnership with more than 35 leading international and regional clean energy organizations.

  15. Renewable Energy for Rural Health Clinics (Energia Removable para Centros de Salud Rurales)

    SciTech Connect

    Jimenez, A. C.; Olson, K.

    This guide provides a broad understanding of the technical, social, and organizational aspects of health clinic electrification, especially through the use of renewable energy sources. It is intended to be used primarily by decision makers within governments or private agencies to accurately assess their health clinic's needs, select appropriate and cost-effective technologies to meet those needs, and to put into place effective infrastructure to install and maintain the hardware. This is the first in a series of rural applications guidebooks that the National Renewable Energy Laboratory (NREL) Village Power Program is commissioning to couple commercial renewable systems with rural applications.more » The guidebooks are complemented by NREL's Village Power Program's development activities, international pilot projects, and visiting professionals program. For more information on the NREL Village Power Program, visit the Renewables for Sustainable Village Power web site at http://www.rsvp.nrel .gov/rsvp/.« less

  16. Centro Nacional de Información de Pesticidas - Portada

    Science.gov Websites

    a.m. a 12:00 p.m. PT, de lunes a viernes A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Índice acuerdo de cooperación entre la Universidad Estatal de Oregón y la Agencia de Protección Ambiental de los Estados Unidos (acuerdo de cooperación # X8-83560101). La información contenida en esta

  17. The acoustic design of the Centro Nacional de las Artes in Mexico City

    NASA Astrophysics Data System (ADS)

    Cooper, Rusell

    2002-11-01

    In this paper the acoustic design of the separate buildings housing the school of music, school of drama, and school of dance that opened in 1996 will be described. Spaces that JHA designed included practice rooms, studios, rehearsal rooms, black box, and concert hall. Details of room acoustic treatments, sound isolation measures, and venturi air flow will be illustrated. An overview of the entire project will also include the 500 seat multipurpose theater (with variable absorption systems) and the Alla Magna. Differences between the American and Mexican styles of consulting, importing of materials, installation, and commissioning will also be discussed.

  18. Birth of piglets from in vitro-produced, zona-intact porcine embryos vitrified in a closed system

    PubMed Central

    Men, Hongsheng; Zhao, Chongbei; Wei, Si; Murphy, Clifton N.; Spate, Lee; Liu, Yang; Walters, Eric M.; Samuel, Melissa S.; Prather, Randall S.; Critser, John K.

    2011-01-01

    As the importance of swine models in biomedical research increases, it is essential to develop low-cost, high-throughput systems to cryopreserve swine germplasm for maintenance of these models. However, porcine embryos are exceedingly sensitive to low temperature and successful cryopreservation is generally limited to the use of vitrification in open systems that allow direct contact of the embryos with liquid nitrogen (LN2). This creates a high risk of pathogen transmission. Therefore, cryopreservation of porcine embryos in a “closed” system is of very high importance. In this study, in vitro-produced (IVP) porcine embryos were used to investigate cryosurvival and developmental potential of embryos cryopreserved in a closed system. Optimal centrifugal forces to completely disassociate intracellular lipids from blastomeres were investigated using Day-4 embryos. Cryosurvival of delipidated embryos was investigated by vitrifying the embryos immediately after centrifugation, or after development to blastocysts. In this study, centrifugation for 30 min at 13,000 g was adequate to completely delipidate the embryos; furthermore, these embryos were able to survive cryopreservation at a rate comparable to those centrifuged for only 12 min. When delipidated embryos were vitrified at the blastocyst stage, there was no difference in survival between embryos vitrified using OPS and 0.25 mL straws. Some embryos vitrified by each method developed to term. These experiments demonstrated that porcine embryos can be cryopreserved in a closed system after externalizing their intracellular lipids. This has important implications for banking swine models of human health and disease. PMID:21458047

  19. Medición de posiciones astrométricas con CCD en la zona de Rup 21

    NASA Astrophysics Data System (ADS)

    Bustos Fierro, I. H.; Calderón, J. H.

    It is shown the utilization of the block adjustment method for the measurement of astrometric positions from a mosaic of sixteen CCD images with partial overlap, which were taken with the Telescope Jorge Sahade of CASLEO. The observations cover an area of 25' x 25' around the open cluster Rup21. The source of reference positions was ACT Reference Catalog. The internal error of the measured positions is analyzed, and the external error is estimated from the comparison with the catalog USNO-A. In this comparison it is found that the direct CCD images taken with focal reducer could be distorted by severe field curvature. The effect of the distortion presumably introduced by the optics is eliminated with the suitable corrections of the stellar positions measured on every frame, but a new systematic effect on scales of the entire field is observed, which could be due to the distribution of the reference stars.

  20. Delimitacion de las Zonas Dialectales de Mexico: Objetivos y Problemas (Delimitation of Dialect Zones in Mexico: Objectives and Problems)

    ERIC Educational Resources Information Center

    Blanch, Juan M. Lope

    1975-01-01

    Before creating a linguistic map of Mexican dialects, it is necessary to determine the dialect regions of the country. An extensive questionnaire must be written and distributed to collect data from a representative sample of the population for an accurate picture of the language. (CK) (Text in Spanish.)

  1. High resolution 40AR/39AR chronostratigraphy of the Late Cretaceous El Gallo Formation, Baja California del Norte, Mexico

    NASA Astrophysics Data System (ADS)

    Renne, Paul R.; Fulford, Madeleine M.; Busby-Spera, Cathy

    1991-03-01

    Laser probe 40Ar/39Ar analyses of individual sanidine grains from four tuffs in the alluvial Late Cretaceous (Campanian) El Gallo Formation yield statistically distinct mean dates ranging from 74.87±0.05 Ma to 73.59±0.09 Ma. The exceptional precision of these dates permits calculation of statistically significant sediment accumulation rates that are much higher than passive sediment loading would cause, implying rapid tectonically induced subsidence. The dates bracket tightly the age of important dinosaur and mammalian faunas previously reported from the El Gallo Formation. The dates support an age less than 73 Ma for the Campanian/Maastrichtian stage boundary, younger than indicated by several currently used time scales. Further application of the single grain 40Ar/39Ar technique may be expected to greatly benefit stratigraphic studies of Mesozoic sedimentary basins and contribute to calibration of biostratigraphic and magnetostratigraphic time scales.

  2. A legacy of change: The lower Colorado River, Arizona-California-Nevada, USA, and Sonora-Baja California Norte, Mexico

    USGS Publications Warehouse

    Mueller, G.A.; Marsh, P.C.; Minckley, W.L.

    2005-01-01

    The lower Colorado is among the most regulated rivers in the world. It ranks as the fifth largest river in volume in the coterminous United States, but its flow is fully allocated and no longer reaches the sea. Lower basin reservoirs flood nearly one third of the river channel and store 2 years of annual flow. Diverted water irrigates 1.5 million ha of cropland and provides water for industry and domestic use by 22 million people in the southwestern United States and northern Mexico. The native fish community of the lower Colorado River was among the most unique in the world, and the main stem was home to nine freshwater species, all of which were endemic to the basin. Today, five are extirpated, seven are federally endangered, and three are being reintroduced through stocking. Decline of the native fauna is attributed to predation by nonnative fishes and physical habitat degradation. Nearly 80 alien species have been introduced, and more than 20 now are common. These nonnative species thrived in modified habitats, where they largely eliminated the native kinds. As a result, the lower Colorado River has the dubious distinction of being among the few major rivers of the world with an entirely introduced fish fauna. ?? 2005 by the American Fisheries Society.

  3. A new derived and highly polymorphic chromosomal race of Liolaemus monticola (Iguanidae) from the 'Norte Chico' of Chile.

    PubMed

    Lamborot, M

    1998-06-01

    A multiple Robertsonian fission chromosomal race of the Liolaemus monticola complex in Chile is described and is shown to be the most derived and the most complex among the Liolaemus examined thus far. The 29 karyotyped lizards analysed from the locality of Mina Hierro Viejo, Petorca, Provincia de Valparaiso, Chile, exhibited a diploid chromosomal number ranging from 42 to 44, and several polymorphisms. The polymorphisms included: a pair 1 fission; a pair 2 fission plus a pericentric inversion in one of the fission products, which moved the NOR and satellite from the tip of the long arm of the metacentric 2 to the short arm of the fission product; a fission in pair 3; a polymorphism for an enlarged chromosome pair 6; and a polymorphism for a pericentric inversion in pair 7. This population is fixed for a fission of chromosome pair 4. A total of 76% of the lizards analysed were polymorphic for one or more pairs of chromosomes. We have compared these data with other Liolaemus monticola chromosomal races and calculated the Hardy-Weinberg ratios for the polymorphic chromosome pairs in this Multiple-Fission race. Karyotypic differences between the Northern (2n = 38-40) and the Multiple-Fission (2n = 42-44) races were attributed mainly to Robertsonian fissions, an enlarged chromosome and pericentric inversions involving the macrochromosomes and one microchromosome pair.

  4. [Opinion of medical and law students of Federal University of Rio Grande do Norte about abortion in Brazil].

    PubMed

    Medeiros, Robinson Dias de; Azevedo, George Dantas de; Oliveira, Emilly Auxiliadora Almeida de; Araújo, Fábio Aires; Cavalcanti, Francisco Jakson Benigno; Araújo, Gabriela Lucena de; Castro, Igor Rebouças

    2012-01-01

    To analyze and compare the knowledge and opinions of Law and Medical students regarding the issue of abortion in Brazil. This was a cross-sectional study involving 125 graduate students from the class of 2010. Of these, 52 were medical students (MED group) and 73 law students (LAW group). A questionnaire was applied based on published research about the topic. Dependent variables were: monitoring the abortion debate, knowledge concerning situations where abortion is permitted under Brazilian law, opinion about situations that agree with extending legal permission to terminate pregnancy and prior knowledge of someone who has undergone induced abortion. Independent variables were: sex, age, household income and graduation course. χ² and Fisher's exact tests, with the level of significance set at 5%. Most interviewees reported monitoring the debate on abortion in Brazil (67.3% of the MED group and 70.2% of the LAW group, p>0.05). When assessing knowledge on the subject, medical students had a significantly higher percentage of correct answers than law students (100.0 and 87.5%, respectively; p=0.005) regarding the legality of abortion for pregnancies resulting from rape. Elevated percentages of correct responses were also recorded for both groups in relation to pregnancies that threaten the life of the mother (94.2 and 87.5% for MED and LAW groups, respectively), but without statistical significance. A significant percentage of respondents declared they were in favor of extending legal abortion to other situations, primarily in cases of anencephaly (68%), pregnancy severely harming the mother's physical health (42.1%) or that of the fetus in cases of severe congenital malformation (33.7%). Results showed a satisfactory knowledge on the part of law and medical school graduate students regarding the legality of abortion in Brazil, combined with a favorable trend towards extending legal permission to other situations not covered by the law. It is important to underscore the inclusion of this topic in the undergraduate curriculum and the development of inter-professional teaching strategies.

  5. Grasslands of Mexico: A perspective on their conservation (Los pastizales del norte de Mexico: Una perspectiva para su conservacion)

    Treesearch

    Patricia Manzano; Rurik List

    2006-01-01

    Grasslands are areas dominated by grasses and herbs with few or no trees. Grasslands receive too much rain for a desert environment and too little for a forest. Temperate North American grasslands, especially, have undergone changes on a continental level. Their high productivity and fertility, added to their level topography and lack of trees, make them ideal sites...

  6. 76 FR 62060 - NortHydro, LLC.; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ... with a powerhouse; (3) two turbine/generator units with a combined capacity of 1,600 kilowatts; (4) a 7.... The wasteway functions as a diversion of surplus water from the irrigation system. The sole purpose of.... The following new facilities are proposed: (1) A diversion structure that would collect water from the...

  7. 76 FR 62060 - NortHydro, LLC; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ...-long underground penstock that would collect water from the Scooteney wasteway; (2) a powerhouse containing one turbine/generator unit with a capacity of 1,110 kilowatts; (3) a 4.2- mile-long, 115-kilovolt.... The wasteway functions as a diversion of surplus water from the irrigation system to the existing...

  8. 76 FR 62061 - NortHydro, LLC; Notice of Preliminary Permit Application Accepted for Filing and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-06

    ... powerhouse; (3) three turbine/generator units with a combined capacity of 1,750 kilowatts; (4) a 7.5-mile... wasteway functions as a diversion of surplus water from the irrigation system. The sole purpose of a... facilities are proposed: (1) A diversion structure that would collect water from the wasteway; (2) three 63...

  9. Geological and geochemical reconnaissance in the central Santander Massif, Departments of Santander and Norte de Santander, Colombia

    USGS Publications Warehouse

    Evans, James George

    1976-01-01

    The central Santander Massif is composed of Precambrian Bucaramanga Gneiss and pre-Devonian Silgara Formation intruded by Mesozoic quartz diorite, quartz monzonite, and alaskite and Cretaceous or younger porphyry. Triassic (Bocas Formation), Jurassic (Jordan and Giron Formations).and Cretaceous (Tambor, Rosa Blanca, Paja, Tablazo, Simiti, La Luna, and Umir Formations) sedimentary rocks overlie the metamorphic rocks and are younger than most of the intrusions. A geological and geochemical reconnaissance of part of the central Santander Massif included the Vetas and California gold districts. At Vetas the gold is generally in brecciated aphanitic quartz and phyllonite. Dark-gray material in the ore may be graphite. The ore veins follow steep west-northwest- and north-northeast-striking fracture zones. No new gold deposits were found. Additional geochemical studies should concentrate on western Loma Pozo del Rey and on improvement of the gold extraction process. At California the gold is in pyritiferous quartz veins and quartz breccia. Ore containing black sooty material (graphite?) is highly radioactive. Some of the mineralization is post-Lower Cretaceous. Soil samples indicate that gold deposits lie under the thick blanket of soil on the ridges above the zone of mining. Three principal gold targets are outlined by gold and associated minerals in pan concentrates. The close relation of gold and copper anomalies suggests that copper may be useful as a pathfinder for gold elsewhere in the region. Based on occurrences of gold or high concentrations of pyrite or chalcopyrite in pan concentrates and on analytical data, eight potential gold targets are outlined in the central massif. Reconnaissance of the surrounding region is warranted.

  10. [Density, size structure and reproductive activity of the pink conch Eustrombus gigas (Mesogastropoda: Strombidae) in Banco Chinchorro, Mexico].

    PubMed

    Cala, Yuself R; Navarrete, Alberto de Jesús; Ocaña, Frank A; Rivera, José Oliva

    2013-12-01

    The pink conch Eustrombus gigas is an important fisheries resource. At the regional level in the Caribbean, over-exploitation and habitat destruction have caused a decrease in the abundance of this resource. In order to provide necessary information for the species management in Mexico, this work aimed to analyze the total density, adult density, size structure and reproductive behavior of pink conch population at Banco Chinchorro during 2009-2010. Data from three seasons were obtained (rainy, dry and cold fronts periods) in three areas: Norte (North), Centro (Center) and Sur (South). The organisms were separated into two groups: (a) the criteria based upon legal harvest in Mexico: legal size conchs (siphonal length > 200 mm) and illegal size conchs (siphonal length < 200 mm), and (b) the criteria based upon sexual maturity using the 15 mm lip thickness standard: lip < 15 mm as juvenile conch and lip > or = 15 mm as adult conch. Copulation, spawning, egg masses and aggregations were evaluated as reproductive evidences. The highest total density was observed during the dry season with 384ind./ha, and the lowest during the rainy season with 127ind./ha. The highest density was reported at Sur (385ind./ha) and the lowest at Norte (198ind./ ha). The highest adult density was observed during the rainy season (8.33ind./ha), and the lowest occurred in the dry season (6.1 ind./ha). Adult density values were 5.55, 7.05 and 8.33ind./ha for Centro, Sur and Norte areas, respectively. Adult densities were lower than the threshold needed for reproduction, and 42% of the population may be vulnerable to fishing, as they had the minimum size for catch (Lsi 200 mm). Furthermore, only 2.2% of the population reached a Gl > 15 mm as sexual maturity indicator. During the study period, only six evidences of reproductive activity were observed. The smaller densities reported at Banco Chinchorro may cause reproduction events to be almost absent which in turn is sufficient evidence to show

  11. The Experimental Projectile Impact Chamber (EPIC) at Centro de Astrobiología, Spain: Reproducibility and verification of scaling relations.

    NASA Astrophysics Data System (ADS)

    Ormö, J.; Wünnemann, K.; Collins, G.; Melero Asensio, I.

    2012-09-01

    The Experimental Projectile Impact Chamber (EPIC) consists of a 20.5mm caliber, compressed gas gun and a 7m wide test bed. It is possible to vary the projectile size and density, the velocity up to about 5001n/"s, the impact angle. and the target composition. The EPIC is especially designed for the analysis of impacts into unconsolidated and liquid targets. i.e. allowing the use of gravity scaling. The general objective with the EPIC is to analyze the cratering and modification processes at wet-target (e.g. marinle) impacts. We have carried out 14 shots into dry sand targets with two projectile compositions (light and weak; heavy and strong), at two impact angles. at three impact velocities, and in both quarter-space and half- space geometries. We recorded the impacts with a high-speed camera and compared the results with numerical simulations using iSALE. The evaluation demonstrated that there are noticeable differences between the results from the two projectile types, but that the crater dimensions are consistent with scaling laws based on other impact experiments [1]. This proves the usefulness of the EPIC in the analysis of natural impacts.

  12. Highly Sensitive Flexible Pressure Sensors Based on Printed Organic Transistors with Centro-Apically Self-Organized Organic Semiconductor Microstructures.

    PubMed

    Yeo, So Young; Park, Sangsik; Yi, Yeon Jin; Kim, Do Hwan; Lim, Jung Ah

    2017-12-13

    A highly sensitive pressure sensor based on printed organic transistors with three-dimensionally self-organized organic semiconductor microstructures (3D OSCs) was demonstrated. A unique organic transistor with semiconductor channels positioned at the highest summit of printed cylindrical microstructures was achieved simply by printing an organic semiconductor and polymer blend on the plastic substrate without the use of additional etching or replication processes. A combination of the printed organic semiconductor microstructure and an elastomeric top-gate dielectric resulted in a highly sensitive organic field-effect transistor (FET) pressure sensor with a high pressure sensitivity of 1.07 kPa -1 and a rapid response time of <20 ms with a high reliability over 1000 cycles. The flexibility and high performance of the 3D OSC FET pressure sensor were exploited in the successful application of our sensors to real-time monitoring of the radial artery pulse, which is useful for healthcare monitoring, and to touch sensing in the e-skin of a realistic prosthetic hand.

  13. Birth of cloned calves from vitrified-warmed zona-free buffalo (Bubalus bubalis) embryos produced by hand-made cloning.

    PubMed

    Saha, Ambikaprasanna; Panda, Sudeepta K; Chauhan, Manmohan S; Manik, Radhey S; Palta, Prabhat; Singla, Suresh K

    2013-01-01

    The availability of techniques for the vitrification of cloned blastocysts can improve their effective use. The present study compared the developmental competence of buffalo cloned embryos derived from adult (BAF), newborn (BNF) and fetal fibroblast (BFF) before and after vitrification. Despite similar cleavage rates among the three groups, the blastocyst rate was lower for BAF- than BNF- and BFF-derived embryos (30.2±2.2% vs 41.7±1.7% and 39.1±2.1%, respectively; P<0.01). The total cell number of BNF-derived blastocysts was significantly higher (P<0.01) than that of BFF-derived blastocysts, which, in turn, was higher (P<0.01) than that of BAF-derived blastocysts. Following transfer of vitrified-warmed blastocysts to recipients, no pregnancy was obtained with fresh (n=8) or vitrified-warmed (n=18) BAF-derived blastocysts, whereas transfer of fresh BNF- (n=53) and BFF-derived (n=32) blastocysts resulted in four and three pregnancies, respectively, which aborted within 90 days of gestation. The transfer of vitrified-warmed BNF-derived blastocysts (n=39) resulted in the live birth of a calf weighing 41kg, which is now 23 months old and has no apparent abnormality, whereas the transfer of vitrified-warmed BFF-derived blastocysts (n=18) resulted in one live birth of a calf that died within 6h. These results demonstrate that cloned buffalo embryos cryopreserved by vitrification can be used to obtain live offspring.

  14. Gender and Gonadal Status Differences in Zona Reticularis Expression in Marmoset Monkey Adrenals: Cytochrome b5 Localization With Respect To Cytochrome P450 17,20-Lyase Activity.

    PubMed Central

    Pattison, J Christina; Saltzman, Wendy; Abbott, David H; Hogan, Brynn K; Nguyen, Ann D; Husen, Bettina; Einspanier, Almuth; Conley, Alan J; Bird., Ian M

    2007-01-01

    Neonatal marmosets express an adrenal fetal zone comparable to humans. While adult males fail to express a functional ZR, with barely detectable blood DHEA levels, females produce higher levels of DHEA than males in adulthood. We investigated the presence of a putative functional ZR in adult female marmosets. In contrast to males, immunohistochemical analysis showed the ZR marker cytochrome b5 was elevated in the innermost zone in cycling females (compared to testis-intact males), further elevated in the adrenals from anovulatory females, and substantially elevated and continuous in ovariectomized females. As a functional test in vivo, following overnight dexamethasone treatment, cycling and anovulatory females showed higher levels of DHEA relative to males, but DHEA failed to increase in response to ACTH. In direct contrast, while ovariectomized females exhibited lower initial DHEA levels, clear increases were detectable after ACTH administration (p<0.05), suggesting an adrenal origin. The apparent differences in cytochrome b5 expression between groups were also further verified by western blotting of adrenal microsomes, and compared to 17,20-lyase activity; the two parameters were positively correlated (p<0.01) across multiple treatment groups. We conclude that the cycling female marmoset expresses a rudimentary ZR with at least a capacity for DHEA production that becomes significantly ACTH-responsive after anovulation. Expression of cytochrome b5 in this region may be directly or indirectly controlled by gonadal function, and is, at least in part, a critical determinant in the development of an adrenal ZR that is more defined and significantly ACTH-responsive. PMID:17222503

  15. Detection of antisperm antibodies: their localization to human sperm antigens that are transferred to the surface of zona-free hamster oocytes during the sperm penetration assay.

    PubMed

    Wiley, L M; Obasaju, M F; Overstreet, J W; Cross, N L; Hanson, F W; Chang, R J

    1987-08-01

    The authors have developed an extension of the sperm penetration assay for detecting serum immunoglobulins to sperm antigens that are transferred to the plasma membrane of a sperm-penetrated hamster oocyte. After the hamster oocytes have been scored for sperm penetration by observing for the presence of swollen sperm heads, they are incubated in serum followed by either a 20-minute treatment with rhodamine-conjugated protein A (which binds to most subclasses of IgA, IgG, and IgM) or a 2-hour incubation in guinea pig serum (complement). Positive fluorescence indicates that the serum contains antibodies to sperm antigens that were transferred to the surface of an oocyte during gamete fusion. Complement-mediated lysis indicates that the immunoglobulin that is bound can also fix complement. The advantages of this assay for detection of serum antisperm antibodies are that it is an extension of a widely used assay, is rapid and requires readily available reagents and equipment, can detect most subclasses of IgA, IgG, and IgM, detects antibodies to those sperm antigens that may be transferred to the oocyte during fertilization, and indicates whether the detected antisperm antibodies can mediate complement-dependent lysis of the fertilized oocyte.

  16. Descripción de la zona de alta difusión en un modelo bidimensional para hojas de corriente

    NASA Astrophysics Data System (ADS)

    Montero, M. F.; Paola, C. A.; Platzeck, A. M.

    Los modelos bidimensionales para describir hojas de corriente de alta simetría no suministran una descripción detallada de las regiones donde la difusión es considerablemente importante. Tal es el caso de los modelos desarrollados en la década del '60 por Sweet, Parker, Petschek y Sonnerup. En esta comunicación presentamos una solución de las ecuaciones MHD en el entorno del origen, construyendo series bidimensionales para todas las magnitudes físicas involucradas. Valiéndonos de la alta simetría y de las características del problema, logramos reducir el número de coeficientes a calcular. Utilizamos un programa autoconsistente para extender el cálculo a regiones vecinas con difusión moderada o despreciable.

  17. Use of medicines and other products for therapeutic purposes among children in Brazil.

    PubMed

    Pizzol, Tatiane da Silva Dal; Tavares, Noemia Urruth Leão; Bertoldi, Andréa Dâmaso; Farias, Mareni Rocha; Arrais, Paulo Sergio Dourado; Ramos, Luiz Roberto; Oliveira, Maria Auxiliadora; Luiza, Vera Lucia; Mengue, Sotero Serrate

    2016-12-01

    átrica brasileira e testar se fatores demográficos, socioeconômicos e médicos estão associados ao uso. Estudo transversal de base populacional (Pesquisa Nacional sobre Acesso, Utilização e Promoção do Uso Racional de Medicamentos - PNAUM), incluindo 7.528 crianças com 12 anos ou menos de idade, residentes na zona urbana do território brasileiro. O uso de medicamentos para tratar doenças crônicas ou agudas foi referido pelo principal cuidador da criança presente na entrevista domiciliar. Associações entre as variáveis independentes e o uso de medicamentos foram investigadas por meio de regressão de Poisson. A prevalência de uso global de medicamentos foi de 30,7% (IC95% 28,3-33,1). A prevalência de uso de medicamentos para doenças crônicas foi de 5,6% (IC95% 4,7-6,7) e para condições agudas, 27,1% (IC95% 24,8-29,4). Os fatores significativamente associados com o uso global foram ter no máximo cinco anos de idade, residir na região Nordeste, ter plano de saúde e utilizar serviços de saúde nos últimos 12 meses (consultas de emergência e internações). Associaram-se ao uso de medicamentos para doenças crônicas: idade ≥ 2 anos, regiões Sudeste e Sul e utilização de serviços de saúde. Para o uso de medicamentos em condições agudas, foram identificados os seguintes fatores associados: ≤ 5 anos, Norte, Nordeste ou Centro-Oeste, plano de saúde e uma ou mais consultas de emergência. Os medicamentos com maior prevalência de uso pelas crianças menores de dois anos foram paracetamol, ácido ascórbico e dipirona; nas crianças com 2 ou mais anos, foram dipirona, paracetamol e amoxicilina. O uso de medicamentos na população infantil é substancial, principalmente no tratamento de condições médicas agudas. As crianças usuárias de medicamentos para doenças crônicas apresentam perfil demográfico diferente das usuárias de medicamentos para condições agudas, em relação ao sexo, à idade e à região geográfica.

  18. Nutrition Knowledge Predicts Eating Behavior of All Food Groups "except" Fruits and Vegetables among Adults in the Paso del Norte Region: Que Sabrosa Vida

    ERIC Educational Resources Information Center

    Sharma, Shreela V.; Gernand, Alison D.; Day, R. Sue

    2008-01-01

    Objective: To examine the association between nutrition knowledge and eating behavior in a predominantly Mexican American population on the Texas-Mexico border. Design: Cross-sectional using data from the baseline survey of the Que Sabrosa Vida community nutrition initiative. Setting: El Paso and surrounding counties in Texas. Participants: Data…

  19. Comportamiento mecanico de la interfase de subduccion durante el ciclo sismico: Estudio mediante la geodesia espacial en el norte de Chile

    NASA Astrophysics Data System (ADS)

    Bejar Pizarro, Marta

    The rampant success of quantum theory is the result of applications of the 'new' quantum mechanics of Schrodinger and Heisenberg (1926-7), the Feynman-Schwinger-Tomonaga Quantum Electro-dynamics (1946-51), the electro-weak theory of Salaam, Weinberg, and Glashow (1967-9), and Quantum Chromodynamics (1973-); in fact, this success of 'the' quantum theory has depended on a continuous stream of brilliant and quite disparate mathematical formulations. In this carefully concealed ferment there lie plenty of unresolved difficulties, simply because in churning out fabulously accurate calculational tools there has been no sensible explanation of all that is going on. It is even argued that such an understanding is nothing to do with physics. A long-standing and famous illustration of this is the paradoxical thought-experiment of Einstein, Podolsky and Rosen (1935). Fundamental to all quantum theories, and also their paradoxes, is the location of sub-microscopic objects; or, rather, that the specification of such a location is fraught with mathematical inconsistency. This project encompasses a detailed, critical survey of the tangled history of Position within quantum theories. The first step is to show that, contrary to appearances, canonical quantum mechanics has only a vague notion of locality. After analysing a number of previous attempts at a 'relativistic quantum mechanics', two lines of thought are considered in detail. The first is the work of Wan and students, which is shown to be no real improvement on the iisu.al 'nonrelativistic' theory. The second is based on an idea of Dirac's - using backwards-in-time light-cones as the hypersurface in space-time. There remain considerable difficulties in the way of producing a consistent scheme here. To keep things nicely stirred up, the author then proposes his own approach - an adaptation of Feynman's QED propagators. This new approach is distinguished from Feynman's since the propagator or Green's function is not obtained by Feynman's rule. The type of equation solved is also different: instead of an initial-value problem, a solution that obeys a time-symmetric causality criterion is found for an inhomogeneous partial differential equation with homogeneous boundary conditions. To make the consideration of locality more precise, some results of Fourier transform theory are presented in a form that is directly applicable. Somewhat away from the main thrust of the thesis, there is also an attempt to explain, the manner in which quantum effects disappear as the number of particles increases in such things as experimental realisations of the EPR and de Broglie thought experiments.

  20. Spatial and temporal distribution of the Asian fish tapeworm Bothriocephalus acheilognathi (Cestoda: Bothriocephalidea) in the Rio Grande (Río Bravo del Norte).

    PubMed

    Bean, Megan G; Bonner, Timothy H

    2010-09-01

    Recent collections of the Asian fish tapeworm Bothriocephalus acheilognathi in the Rio Grande have raised concern about the potential impacts on Rio Grande endemic and imperiled fishes. The objectives of this study were to determine distribution and definitive hosts of the Asian fish tapeworm within the Rio Grande drainage and to quantify occurrences and abundances. In total, 1,992 fish spanning 11 families were collected and examined for Asian fish tapeworms in the Rio Grande and the Pecos and Devils rivers. The parasite was collected from red shiners Cyprinella lutrensis, Tamaulipas shiners Notropis braytoni, sand shiners N. stramineus, river carpsuckers Carpiodes carpio, plains killifish Fundulus zebrinus, western mosquitofish Gambusia affinis, blue suckers Cycleptus elongatus, blacktail shiners Cyprinella venusta, proserpine shiners Cyprinella proserpina, and Manantial roundnose minnow Dionda argentosa, with the latter four species being new host records. Monthly collections of red shiners from Big Bend National Park exhibited prevalence levels above 15% in January-March and December and below 10% during April-June and October. With over 50% of the Rio Grande icthyofauna in Texas considered imperiled, the occurrence and pathological effects of the Asian fish tapeworm in combination with reduced water quantity and quality and increased habitat fragmentation are of concern for these taxa.

  1. Estructura y química del suelo en un bosque de Castilla elastica en el carso del norte de Puerto Rico: resultados de una calicata

    Treesearch

    Christian A. Viera Martinez; Oscar Abelleira; Ariel E. Lugo

    2008-01-01

    We dug a soil pit of 1m x 1m x 1m in a forest dominated by Castilla elastica, a tree for shade coffee introduced in the karst of northern Puerto Rico. We found four soil horizons (designation notes in parenthesis) (A) organic soil matter (E) mineral soil leachate (B) aerobic mineral soil, and (C) saturated soil. The total storage of soil organic matter was 143 Mg/ha....

  2. Utilização Do Sensor Airdas (Airborne Infrared Disaster Assessment System) no monitoramento de desflorestamentos no norte do estado do mato grosso - Brasil

    Treesearch

    Geraldo José Lucatelli Dória de Araújo; João Antônio Raposo Pereira; Tânia Maria Vieira da Silva; Helvécio Mafra; James A. Brass; Robert N. Lockwood; Robert G. Higgins; Philip J. Riggan

    2008-01-01

    This study has as objective to assess AIRDAS (Airborne Infrared Disaster Assessment System) sensor for the monitoring of deforestation in the northern area of Mato Grosso State, between the latitudes 10° and 12° south and longitudes 54° and 56° west, within the area known as deforestation arch. The results show that the main advantage of...

  3. [Atypical mucocutaneous manifestations in neonates and infants with chikungunya fever in the municipalities of Cúcuta, Los Patios and Villa del Rosario, Norte de Santander, Colombia, 2014].

    PubMed

    Muñoz, Claudia Marcela; Castillo, José Orlando; Salas, Daniela; Valderrama, Milena Alexandra; Rangel, Claudia Teresa; Vargas, Heiddy Patricia; Silva, Diana Carolina

    2016-09-01

    Atypical clinical manifestations have been observed in newborns and infants suffering from fever caused by the chikungunya virus. Objective: To describe the cases of fever caused by the chikungunya virus in newborns and infants with atypical mucocutaneous lesions. Materials and methods: We reviewed the clinical records, as well as lab tests and histopathological results, of newborns and infants diagnosed with Chikungunya virus and atypical mucocutaneous lesions in three regional hospitals. Results: Out of 18 suspected cases of chikungunya virus in newborns and infants, 11 were positive and presented atypical mucocutaneous manifestations. Six of the eleven confirmed cases corresponded to children under five months of age. The most common symptoms were fever, skin rash, irritability, and diarrhea. Three of the patients were infected with both dengue and chikungunya viruses. The ulcers occurred in the scalp, abdomen, genital and perianal region. We report mucocutaneous manifestations in newborns and infants diagnosed with fever caused by the chikungunya virus in Colombia. The rapid development of ulcers is most likely due to the immune response to the virus. Special attention should be given to pregnant women presenting symptoms of chikungunya virus infection prior to delivery, and their offspring should be followed-up in order to monitor possible complications.

  4. Decrease of the incidence of human and canine visceral leishmaniasis after dog vaccination with Leishmune in Brazilian endemic areas.

    PubMed

    Palatnik-de-Sousa, Clarisa B; Silva-Antunes, Ilce; Morgado, Adilson de Aguiar; Menz, Ingrid; Palatnik, Marcos; Lavor, Carlile

    2009-06-02

    Leishmune, the first prophylactic vaccine licensed against canine visceral leishmaniasis (CVL), has been used in Brazil since 2004, where seropositive dogs are sacrificed in order to control human visceral leishmaniasis (VL). We demonstrate here that vaccination with Leishmune does not interfere with the serological control campaign (110,000 dogs). Only 1.3% of positivity (76 among 5860) was detected among Leishmune uninfected vaccinees. We also analyzed the possible additive effect of Leishmune vaccination over dog culling, on the decrease of the incidence of CVL and VL in two Brazilian endemic areas, from 2004 to 2006. In Araçatuba, a 25% of decline was seen in CVL with a 61% decline in human cases, indicating the additive effect of Leishmune vaccination of 5.7% of the healthy dogs (1419 dogs), on regular dog culling. In Belo Horizonte (BH), rising curves of canine and human incidence were observed in the districts of Barreiro, Venda Nova and Noroeste, while the canine and human incidence of Centro Sul, Leste, Nordeste, Norte, Pampulha and Oeste, started to decrease or maintained a stabilized plateau after Leishmune vaccination. Among the districts showing a percent decrease of human incidence (-36.5%), Centro Sul and Pampulha showed the highest dog vaccination percents (63.27% and 27.27%, respectively) and the lowest dog incidence (-3.36% and 1.89%, respectively). They were followed by Oeste, that vaccinated 25.30% of the animals and experienced an increase of only 12.86% of dog incidence and by Leste and Nordeste, with lower proportions of vaccinees (11.72% and 10.76%, respectively) and probably because of that, slightly higher canine incidences (42.77% and 35.73%). The only exception was found in Norte district where the reduced human and canine incidence were not correlated to Leishmune vaccination. Much lower proportions of dogs were vaccinated in Venda Nova (4.35%), Noroeste (10.27%) and Barreiro (0.09%) districts, which according to that exhibited very

  5. Negligent and intentional fires in Portugal: the role of human and biophysical drivers on the temporal distribution

    NASA Astrophysics Data System (ADS)

    Parente, Joana; Pereira, Mário; Amraoui, Malik; Tedim, Fantina

    2017-04-01

    Portugal is the European country with higher number of fires (NF) and burnt area (BA) per unit of land area. The annual number of fires for which the cause of fire is known is not constant and relatively small (typically less than 50% of annual number of records). Nevertheless, the analysis of the fire causes reveals that the vast majority (99%) of the fires in Portugal are of human origin and only a small fraction are of natural origin (1% caused by lightning). The study period will be the recent years of 2012 - 2014, when fire recording procedures are more reliable and the cause of ignition was assessed for more than 50% (19376) of the fires. The fires with approximately seventy different causes of fire defined/recognized by the Portuguese Forest Service (ICNF) were grouped into negligent, intentional and natural fires. For this study the authors proposes the use of the Nomenclature of Territorial Units for Statistics level II, which divides Portugal in 5 basic economic regions, namely Norte, Centro, Área Metropolitana de Lisboa, Alentejo, and Algarve. Most of the fires (54%) occur in the so-called critical period defined between July and September, but high wildfire activity may also occur in few periods of the remaining months (especially in February and March). The intentional fires represent 40% of total NF but accounts for 53% of total BA during the study period. The temporal distribution are described and interpreted in terms of the climate, fire weather, land use land cover (LULC), distance to communication routes (roads and railways) and topographic variables (altitude, slope) using statistical analysis and GIS techniques. Results points to: a) higher number of negligent than intentional fires; b) higher BA on critical period in years 2012 and 2013; c) decrease in time and decrease from critical period to non-critical period of the number of fires, in all regions; and d) the dominant role of fire weather in the observed temporal patterns. We strongly

  6. Negligent and intentional fires in Portugal: the role of human and biophysical drivers on the spatial distribution

    NASA Astrophysics Data System (ADS)

    Parente, Joana; Pereira, Mário; Amraoui, Malik; Tedim, Fantina

    2017-04-01

    The European Mediterranean countries, such as Portugal, Spain, France, Italy and Greece, have the higher incidence of fire. Of these countries, Portugal present the highest average number of fires (NF) and one of the highest burnt area (BA), in spite of its relatively smaller land area. The study period is focused in the recent years of 2012 - 2014, when a total of 59 257 fires were recorded and the fire cause is known for more than 50% of the fire records. All fires with known causes were then classified into intentional (40% of the total number of fires) and negligent (60%), leading to a total of 45% of fires related with human factors and activities. Taking into account these values the authors believe it's necessary to better understand the fire regime of this type of fires for a better fire prevention, firefighting and crisis management. Accordingly, the use of statistical analysis and GIS techniques were used to assess the spatial distribution of the human caused fires in each of the NUTS (Nomenclature of Territorial Units for Statistics level I, which divides Portugal in 5 basic economic regions, namely Norte, Centro, Area Metropolitana de Lisboa, Alentejo, and Algarve. The number of fires distribution increases with latitude, making north of Portugal the region with the highest number of fires. The analysis will also aims to assess the role of the most important human and biophysical drivers of the spatial distribution, namely the population density, land use land cover (LULC), distance to communication routes (roads and railways) and topographic variables (altitude, slope). The results show that: a) population density is highly and positively correlated with the agglomeration of fire ignitions, but doesn't imply highest burned area; b) burnt area increase with the distance to roads and altitude; and, c) 58% of the fires occurred on agriculture areas and 33% of fires occurred in forest and scrubs areas. Acknowledgements: This work was supported by: (i) the

  7. Short report: screening for Trypanosoma cruzi in the blood supply by the Red Cross blood bank in Quito, Ecuador.

    PubMed

    Grijalva, M J; Chiriboga, R; Racines, J R; Escalante, L; Rowland, E C

    1997-12-01

    The status of Chagas' disease in Ecuador is not clear. In response to reports suggesting the possibility of transfusion-associated transmission of Chagas' disease in the blood bank in Quito, the Ecuadorian Red Cross in collaboration with the Instituto Nacional de Higiene, Zona Norte and the Tropical Disease Institute of Ohio University implemented a pilot Chagas' disease screening of the donated blood in the Quito blood bank. The results of the screening showed a low incidence of seropositivity among the donors (0.01% in 1994, 0.04% in 1995, and 0.02% in 1996) to the Quito blood bank and a higher seropositivity in samples donated to smaller blood banks (0.4% in 1994, 0.28% in 1995, and 0.13% in 1996) located in areas considered endemic, as well as from at least two areas previously considered nonendemic for Chagas' disease. This report highlights the need for a comprehensive evaluation of the prevalence and distribution of Chagas' disease in Ecuador.

  8. Prevalência de tromboembolismo pulmonar incidental em pacientes oncológicos: análise retrospectiva em grande centro

    PubMed Central

    Carneiro, Renata Mota; van Bellen, Bonno; Santana, Pablo Rydz Pinheiro; Gomes, Antônio Carlos Portugal

    2017-01-01

    Resumo Contexto Devido à maior aplicação de exames de imagem rotineiros, especialmente nos pacientes com neoplasia para controle da doença, vem aumentando o diagnóstico de tromboembolismo pulmonar (TEP) incidental, importante fator de morbimortalidade associado. Objetivo Identificar os casos de TEP incidental em pacientes oncológicos submetidos a tomografia computadorizada (TC) de tórax, correlacionando aspectos clínicos e fatores de risco associados. Métodos Estudo retrospectivo de todos os episódios de TEP ocorridos de janeiro de 2013 a junho de 2016, com seleção dos pacientes oncológicos e divisão deles em dois grupos: com suspeita clínica e sem suspeita clínica (incidentais) de embolia pulmonar. Resultados Foram avaliados 468 pacientes com TEP no período citado. Destes, 23,1% eram oncológicos, entre os quais 44,4% apresentaram achado incidental de embolia pulmonar na TC de tórax. Não houve diferença estatística entre os grupos para sexo, idade e tabagismo. Quanto à procedência, 58,3% dos pacientes sem suspeita clínica eram de origem ambulatorial e 41,7% com suspeita de TEP vinham do pronto-socorro (p < 0,001). As neoplasias mais prevalentes foram de pulmão (17,6%), intestino (15,7%) e mama (13,0%). Aqueles com achado incidental apresentaram significativamente mais metástases, sem diferença entre os grupos para realização de quimioterapia, radioterapia ou cirurgia recente. Quanto aos sintomas apresentados, 41,9% daqueles sem suspeita clínica tinham queixas sugestivas de TEP quando realizaram o exame. Conclusão TEP incidental é frequente em pacientes oncológicos, especialmente naqueles provenientes de seguimento ambulatorial e em estágios avançados da doença. Sintomas sugestivos de TEP estavam presentes em pacientes sem suspeita clínica ao realizarem a TC de tórax. PMID:29930652

  9. [Predictive factors of neurological complications in the period immediately after liver transplant: experiences in the Centro de Investigaciones Médico Quirúrgicas in Cuba].

    PubMed

    Abdo-Cuza, A; González-Rapado, L; López-Cruz, O; Pérez-Bernal, J; Castellanos-Gutiérrez, R; Gómez-Peyre, F; Hinojosa-Pérez, R; Lage-Dávila, J; Alvarez-Rodríguez, A; Fernández-Valle, A; Fernández-Maderos, I; Samada-Suárez, M; Hernández-Perera, J C; Bernardos-Rodríguez, A

    Liver transplant (LT) is today a first choice procedure in a group of hepatic diseases in their acute and chronic terminal stages. It is not, however, a technique that is completely free of complications and those of a neurological nature constitute between 8 47% of those reported. AIMS. The purpose of this study is to present the immediate neurological complications (NC) found in our patients, as well as to determine the predictive factors and their relation to the mortality rate. From the medical records of 26 patients who received LT at the CIMEQ (July 1999 December 2001), we collected a group of variables related to the donor, the surgical procedure and the post operative period and associated them to the occurrence of NC while these patients were in the ICU. NC were found in 16 patients (61.5%), the most frequent being encephalopathy (30.8%), tremor (26.9%), and convulsions (19.2%). No relation was found between the presence of NC and prior hepatic encephalopathy, the use of a suboptimal donor, nor did it represent a significant increase in the mortality rate. There was a significant relation with LT to recipients rated as grade C on the Child Pugh classification system, the presence of intraoperative hypotension (p= 0.0164) and primary dysfunction of the liver graft (p= 0.041). NC represented a significant cause of morbidity in the period following a liver transplant in our series, although they had no significant repercussion on the mortality rate. Their presence is related to variables concerning the recipient, the surgical procedure itself and the immediate post operative period.

  10. Directrices para la Creacion del Centro Nacional de Informacion y Documentacion (Guidelines for the Creation of a National Information and Documentation Center).

    ERIC Educational Resources Information Center

    Menda Behrens, Eduardo

    This document discusses background information and guidelines for the creation of a national information and documentation center for the dissemination of scientific and technological information. The study first reports on information services in several other countries and the need for an information center in Venezuela; included are…

  11. Summary Report for National Aeronautics Space Administration (NASA) and Centro Para Prevencao da Poluicao (C3P) 2011 International Workshop on Environment and Alternative Energy

    NASA Technical Reports Server (NTRS)

    Greene, Brian

    2011-01-01

    The C3P &. NASA International Workshop on Environment and Alternative Energy was held on November 15-18, 2011 at the European Space Agency (ESA)'s Research and Technology Centre (ESTEC) in Noordwijk, The Netherlands. The theme of the workshop was "Global Collaboration in Environmental and Alternative Energy Strategies". The workshop was held at ESTEC's conference center. More than 110 individuals from eleven countries attended the workshop. For the first time since the inception of NASA-C3P workshops, a full day was dedicated to a student session. Fifteen students from around the globe gave oral presentations along with poster displays relating to the latest technologies in environmental and alternative energy strategies. Judges from NASA, C3P and ESA awarded plaques to the top three students. In addition to the students, thirty eight U.S. and international subject matter experts presented on the following general environmental-related topics: (1) Hazardous materials management and substitution in support of space operations (2) Emerging renewable and alternative energy technologies (3) Sustainable development and redevelopment (4) Remediation technologies and strategies The workshop also included a panel discussion on the topic of the challenges of operating installations across borders. Throughout the workshop, attendees heard about the scope of environmental and energy challenges that industry and governments face. They heard about technologies for increasing energy efficiency and increasing use of renewable energy. They learned about ways companies and government agencies are using materials, processes, goods and services in a manner more respectful with the environment and in compliance with health and safety rules. The concept of partnerships and their inherent benefits was evidenced throughout the workshop. Partnering is a key aspect of sustainability because sustainable development is complicated. Through formal presentations and side discussions, attendees commented on the need for continued exploration of joint projects of mutual interest.

  12. [Evolution of residual risk for HIV, HCV and HBV, from 1999 to 2010, in blood donations of the Centro Hospitalar S. João, EPE, Porto, Portugal].

    PubMed

    Koch, Carmo; Araújo, Fernando

    2013-01-01

    Monitoring the residual risk of transfusion-transmitted viral infections is important to evaluate the improvement achieved in the blood donation safety and to adopt policies to reduce risks. The present study calculates the incidence of the key infectious diseases, human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV) as well as the residual risk of transfusion-transmitted viral infections, during twelve years, 1999 through 2010. Data were analyzed over 3 periods of 4 years (1999-2002, 2003-2006 and 2007-2010). The risk estimates were compared to those previously obtained for blood donations occurred between 1991 and 1998. The study included 209 640 blood donations, from 42 634 regular, volunteers and unpaid donors. The residual risk of transfusion-transmitted infection per million donations was calculated, for each virus, through mathematical model "Incidence rate/window period", described by Schreiber et al. All donations were screened according to Portuguese legislation. In January 2001, the nucleic acid testing in minipool was implemented on all blood donations, for screening simultaneously HIV-1 and HCV ribonucleic acid (RNA) (Cobas Amplicor Ampliscreen-Roche©). This test was replaced, in January 2007, by the simultaneous screening of HBV deoxyribonucleic acid, HCV RNA and HIV-1/HIV-2 RNA, in minipool (Cobas Taqscreen MPX Test-Roche©). The residual risk of transmitting viral infections during the transfusion of blood components is very small and has declined over the years. After the implementation of the nucleic acid testing in minipool for the three viruses, the risk of giving blood during an infectious window period was estimated as follows: for human immunodeficiency virus, 1 in 1.67 million, for hepatitis C virus 1 in 3.33 million and for hepatitis B virus 1 in 526 000. During the 12 years under study, we found a decrease in residual risk for the three viruses, by a factor around five for human immunodeficiency virus and hepatitis B virus, and 32 for hepatitis C virus. If we compare the estimates previously calculated for 1991-1998 period to 2007-2010 period (over 20 years), the decrease is still more relevant with a residual risk of human immunodeficiency virus, hepatitis B virus and hepatitis C virus respectively 19-fold, 6-fold and 54-fold lower.

  13. Aeromagnetic maps of the Colorado River region including the Kingman, Needles, Salton Sea, and El Centro 1 degree by 2 degrees quadrangles, California, Arizona, and Nevada

    USGS Publications Warehouse

    Mariano, John; Grauch, V.J.

    1988-01-01

    Aeromagnetic anomalies are produced by variations in the strength and direction of the magnetic field of rocks that include magnetic minerals, commonly magnetite. Patterns of anomalies on aeromagnetic maps can reveal structures - for example, faults which have juxtaposed magnetic rocks against non-magnetic rocks, or areas of alteration where magnetic minerals have been destroyed by hydrothermal activity. Tectonic features of regional extent may not become apparent until a number of aeromagnetic surveys have been compiled and plotted at the same scale. Commonly the compilation involves piecing together data from surveys that were flown at different times with widely disparate flight specifications and data reduction procedures. The data may be compiled into a composite map, where all the pieces are plotted onto one map without regard to the difference in flight elevation and datum, or they may be compiled into a merged map, where all survey data are analytically reduced to a common flight elevation and datum, and then digitally merged at the survey boundaries. The composite map retains the original resolution of all the survey data, but computer methods to enhance regional features crossing the survey boundaries may not be applied. On the other hand, computer methods can be applied to the merged data, but the accuracy of the data may be slightly diminished.

  14. The Expanding Context of European and Mediterranean Security: A Joint Project Between the Center for Naval Analyses (CNA) and Centro Militare di Studi Strategici (CeMiSS)

    DTIC Science & Technology

    2007-04-01

    has deployed several police-type assistance missions. The UN’s Food and Agriculture Organization (FAO), has, in the broadest of senses, a "security...34 mandate, inasmuch as food shortages in the world ineluctably are a direct cause of insecurity, which, in turn, is likely to require response by...failure of international institutions, like the International Maritime Organization, to address the environmental and safety impacts of shipping on coastal

  15. The Expanding Context of European and Mediterranean Security: A Joint Project Between the Center for Naval Analyses (CNA) and Centro Militare di Studi Strategici (CeMiSS)

    DTIC Science & Technology

    2007-07-01

    deployed several police-type assistance missions. The UN’s Food and Agriculture Organization (FAO), has, in the broadest of senses, a "security" mandate...inasmuch as food shortages in the world ineluctably are a direct cause of insecurity, which, in turn, is likely to require response by "security... safety impacts of shipping on coastal states 5. Yale University Press (1962) 57 6.3 Modern challenges preventing achievement of desired end states * Flag

  16. Tão perto de casa, tão longe de nós: etnografia das novas margens no centro da urbe

    PubMed Central

    Fernandes, Por Luís

    2011-01-01

    Philippe Bourgois é, desde 2007, “Richard Perry University professor” no Departamento de Antropologia e de Medicina Familiar e de Práticas Comunitárias na Universidade da Pensilvânia. Esteve durante largos anos ligado ao Departamento de Antropologia, História e Medicina Social da Universidade da Califórnia, São Francisco. A publicação, em 1995, de In Search of Respect: Selling Crack in El Barrio1 projectaria o seu nome muito para além dos Estados Unidos: uma etnografia no coração porto-riquenho do Harlem, em torno dos actores e dos ambientes da venda de crack. Seguir-se-ia um longo trabalho de terreno em acampamentos de dependentes de heroína em São Francisco, orientando o seu trabalho para as formas mais radicais da pobreza e da marginalidade nos EUA. É deste trabalho de terreno que sai o seu último livro, Righteous Dopefiend2. Em Junho de 2007 esteve em Lisboa para participar na 3.a edição do “Ethnografeast”. Foi então que aproveitámos a oportunidade para ouvir um percurso invulgar contado pelo próprio: uma longa conversa no Hotel Zurique, cujo nome só vem ao caso por evocar o país onde passou uma parte da infância. PMID:22013286

  17. Are there any advantages in using a distal aiming device for tibial nailing? Comparing the Centro Nailing System with the Unreamed Tibia Nail.

    PubMed

    Veen, Egbert J D; Ettema, Harmen B; Zuurmond, Rutger G; Mostert, Adriaan K

    2011-10-01

    The distal locking of an intramedullary tibial nail can be challenging and time consuming when performed freehand. This study was conducted to evaluate if a distal aiming device would reduce surgical time. A case-controlled study was performed between 2007 and 2009 with 30 patients receiving a reamed tibial nail (Centronail) with the use of a distal aiming device and 30 patients who were treated with an Unreamed Tibia Nail (UTN), with freehand distal locking, in the same period. The primary outcome in this study was operative time. Secondary outcomes were the need for fluoroscopy, time to consolidation and complications. Operation time was longer in the Centronail group compared with the UTN group (126 min vs. 96 min, p=0.000). Use of fluoroscopy for distal locking was needed in half of the cases (n=16) using a distal aiming device. No differences were found regarding time to consolidation, time to removal of the nail and complications. The use of an aiming device for distal locking of a tibia nail lengthens operation time rather than reducing it. Fluoroscopy was still needed in about half of the cases. No difference was seen in clinical outcomes. The use of a distal aiming device to lock a tibial nail appears to have no benefit. Copyright © 2011 Elsevier Ltd. All rights reserved.

  18. La Desercion Escolar en los Niveles de Sexto y Noveno Grado: Una Comparacion Entre Zonas Rurales y Urbanas. Boletin 219 (The Educational Desertion in the 6th and 9th Grades: A Comparison Between Rural and Urban Zones. Bulletin 219).

    ERIC Educational Resources Information Center

    Lopez, Maria I. Hernandez; And Others

    In 1962 and again in 1965, a group of sixth and ninth grade students in selected schools in four Puerto Rican communities were interviewed to investigate the factors influencing students' decision to withdraw from school before completing twelfth grade and to identify some characteristics of dropouts. Of 616 students interviewed in 1962, 20.9% of…

  19. Understanding Changes in Water Availability in the Rio Grande/Rio Bravo del Norte Basin Under the Influence of Large-Scale Circulation Indices Using the Noah Land Surface Model

    NASA Technical Reports Server (NTRS)

    Khedun, C. Prakash; Mishra, Ashok K.; Bolten, John D.; Beaudoing, Hiroko K.; Kaiser, Ronald A.; Giardino, J. Richard; Singh, Vijay P.

    2012-01-01

    Water availability plays an important role in the socio-economic development of a region. It is however, subject to the influence of large-scale circulation indices, resulting in periodic excesses and deficits. An assessment of the degree of correlation between climate indices and water availability, and the quantification of changes with respect to major climate events is important for long-term water resources planning and management, especially in transboundary basins as it can help in conflict avoidance. In this study we first establish the correlation of the Pacific Decadal Oscillation (PDO) and El Nino-Southern Oscillation (ENSO) with gauged precipitation in the Rio Grande basin, and quantify the changes in water availability using runoff generated from the Noah land surface model. Both spatial and temporal variations are noted, with winter and spring being most influenced by conditions in the Pacific Ocean. Negative correlation is observed at the headwaters and positive correlation across the rest of the basin. The influence of individual ENSO events, classified using four different criteria, is also examined. El Ninos (La Ninas) generally cause an increase (decrease) in runoff, but the pattern is not consistent; percentage change in water availability varies across events. Further, positive PDO enhances the effect of El Nino and dampens that of La Nina, but during neutral/transitioning PDO, La Nina dominates meteorological conditions. Long El Ninos have more influence on water availability than short duration high intensity events. We also note that the percentage increase during El Ninos significantly offsets the drought-causing effect of La Ninas.

  20. Mesohabitats, fish assemblage composition, and mesohabitat use of the Rio Grande silvery minnow over a range of seasonal flow regimes in the Rio Grande/Rio Bravo del Norte, in and near Big Bend National Park, Texas, 2010-11

    USGS Publications Warehouse

    Moring, J. Bruce; Braun, Christopher L.; Pearson, Daniel K.

    2014-01-01

    There were no statistically significant differences between the stream velocities associated with seine hauls of the Rio Grande silvery minnow and Tamaulipas shiner. Stream velocities associated with the seine hauls that included Rio Grande silvery minnow indicate that this species is predominantly found in low-velocity mesohabitats. Velocities associated with seine hauls that included the Tamaulipas shiner represented a much broader overall range of velocities than those associated with Rio Grande silvery minnow collections. No statistically significant differences were found between the depths for seine hauls that included Rio Grande silvery minnow or Tamaulipas shiner. The Rio Grande silvery minnow was more commonly collected in seine hauls from mesohabitats dominated by cobble substrates and less frequently collected in mesohabitats with substrates dominated by fine-sized silt and clay particles, gravels, and sands, in that order. In contrast, the Tamaulipas shiner was broadly distributed among mesohabitats characterized as having gravel, cobble, and silt and clay.

  1. Emergent and Reemergent Arboviruses in South America and the Caribbean: Why So Many and Why Now?

    PubMed

    Marcondes, Carlos Brisola; Contigiani, Marta; Gleiser, Raquel Miranda

    2017-05-01

    Varios arbovirus han emergido y/o reemergido en el Nuevo Mundo en las últimas décadas. Los virus Zika y chikungunya, anteriormente restringidos a África y quizás Asia, invadieron el continente, causando gran preocupación; además siguen ocurriendo brotes causados por el virus dengue en casi todos los países, con millones de casos por año. El virus West Nile invadió rápidamente América del Norte, y ya se han encontrado casos en América Central y del Sur. Otros arbovirus, como Mayaro y el virus de la encefalitis equina del este han aumentado su actividad y se han encontrado en nuevas regiones. Se han documentado cambios en la patogenicidad de algunos virus que conducen a enfermedades inesperadas. Una fauna diversa de mosquitos, cambios climáticos y en la vegetación, aumento de los viajes, y urbanizaciones no planificadas que generan condiciones adecuadas para la proliferación de Aedes aegypti (L.), Culex quinquefasciatus Say y otros mosquitos vectores, se han combinado para influir fuertemente en los cambios en la distribución y la incidencia de varios arbovirus. Se enfatiza la necesidad de realizar estudios exhaustivos de la fauna de mosquitos y modificaciones de las condiciones ambientales, sobre todo en las zonas urbanas fuertemente influenciadas por factores sociales, políticos y económicos. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  2. Astronomía Gamma desde El Leoncito

    NASA Astrophysics Data System (ADS)

    Rovero, A. C.; Colombo, E.; Sahade, J.; Weekes, T. C.

    La astronomía gamma, en el extremo de energías detectadas hasta el presente, se encuentra en desarrollo en muchas partes del mundo y proporciona resultados sorprendentes a medida que la tecnología avanza en el desarrollo de nuevos detectores. La técnica Cherenkov Atmosférica es la utilizada para la detección, mediante telescopios en tierra, del efecto secundario causado por la radiación gamma de muy altas energías (E ≈ TeV). Por medio de telescopios o arreglos de detectores y utilizando la técnica mencionada, se han podido detectar algunas fuentes puntuales de mucha importancia astrofísica como la Nebulosa del Cangrejo y la galaxia de núcleo activo Markarian 421. El sistema instalado en San Juan, en cooperación con el grupo de Radiación Gamma del Observatorio Whipple, está constituído por tres colectores de 1.5m que registran pulsos de luz generados por las cascadas de partículas producidas por rayos cósmicos de todo tipo al entrar en la atmósfera terrestre. El sistema opera por barrido en ascención recta de la fuente observada que, para esta etapa del experimento, es el Centro Galáctico. Esta fuente extensa es conocida como emisor gamma de energías inferiores al TeV y ha sido observada anteriormente en este rango de energía desde el hemisferio norte pero con elevaciones bajas, situación no óptima para este tipo de técnica. Se presentan los primeros resultados del análisis de los datos obtenidos observando la región del Centro Galáctico, luego de dos años de observaciones, así como una descripción de la técnica Cherenkov Atmosférica y del equipo utilizado en la experiencia.

  3. The AstraLux Large M-dwarf Multiplicity Survey

    NASA Astrophysics Data System (ADS)

    Janson, Markus; Hormuth, Felix; Bergfors, Carolina; Brandner, Wolfgang; Hippler, Stefan; Daemgen, Sebastian; Kudryavtseva, Natalia; Schmalzl, Eva; Schnupp, Carolin; Henning, Thomas

    2012-07-01

    We present the results of an extensive high-resolution imaging survey of M-dwarf multiplicity using the Lucky Imaging technique. The survey made use of the AstraLux Norte camera at the Calar Alto 2.2 m telescope and the AstraLux Sur camera at the ESO New Technology Telescope in order to cover nearly the full sky. In total, 761 stars were observed (701 M-type and 60 late K-type), among which 182 new and 37 previously known companions were detected in 205 systems. Most of the targets have been observed during two or more epochs, and could be confirmed as physical companions through common proper motion, often with orbital motion being confirmed in addition. After accounting for various bias effects, we find a total M-dwarf multiplicity fraction of 27% ± 3% within the AstraLux detection range of 0farcs08-6'' (semimajor axes of ~3-227 AU at a median distance of 30 pc). We examine various statistical multiplicity properties within the sample, such as the trend of multiplicity fraction with stellar mass and the semimajor axis distribution. The results indicate that M-dwarfs are largely consistent with constituting an intermediate step in a continuous distribution from higher-mass stars down to brown dwarfs. Along with other observational results in the literature, this provides further indications that stars and brown dwarfs may share a common formation mechanism, rather than being distinct populations. Based on observations collected at the European Southern Observatory, Chile, under observing programs 081.C-0314(A), 082.C-0053(A), and 084.C-0812(A), and on observations collected at the Centro Astronómico Hispano Alemán (CAHA) at Calar Alto, operated jointly by the Max-Planck Institute for Astronomy and the Instituto de Astrofísica de Andalucía (CSIC).

  4. Analysis of admixture proportions in seven geographical regions of the state of Guerrero, Mexico

    PubMed Central

    Cahua‐Pablo, José Ángel; Cruz, Miguel; Tello‐Almaguer, Pedro Vidal; del Alarcón‐Romero, Luz Carmen; Parra, Esteban Juan; Villerías‐Salinas, Salvador; Valladares‐Salgado, Adán; Tello‐Flores, Vianet Argelia; Méndez‐Palacios, Abigail; Pérez‐Macedonio, Claudia Paola

    2017-01-01

    Abstract Objective Mexico's current population structure has been defined by admixture between European, Native American, and to some extent African, groups that started in the sixteenth century. The aim of this research was to analyze the relative contributions of these continental population groups to the seven regions of the state of Guerrero, Mexico. Methods A total of 104 ancestry informative markers were analyzed in 480 unrelated women from the seven regions of the state of Guerrero. The individual ancestry proportions were estimated using the software ADMIXMAP v3.2. Results The relative Native American, European and African ancestral contributions to the whole sample were estimated to be 69%, 27%, and 1.9%, respectively. We observed significant differences in admixture proportions across the regions. The highest average Native American ancestry was found in the Montaña region and the lowest in Costa Grande. Conversely, the highest European contribution was observed in Costa Grande. The highest African contributions were observed in the regions of Costa Chica and Costa Grande. Conclusions The genetic structure of the population of Guerrero reflects quite well the historical processes that have occurred in this state. Native American population settlements were mainly in the regions of Montaña, Norte, and Centro, where the highest indigenous genetic contribution is observed today. European settlers came from the center of the state to regions with significant agricultural and mining activities. The highest African contributions are observed in coastal regions, in agreement with historical evidence about slave trade routes in the Americas. PMID:28675593

  5. Urinary retention, erectile dysfunction and meningitis due to sacral herpes zoster: a case report and review of the literature.

    PubMed

    Erol, B; Avci, A; Eken, C; Ozgok, Y

    2009-01-01

    Zona zoster infection is often associated with painful erythematous vesicular eruptions of the skin or mucous membranes. Varicella zoster virus which stays latent in the sensorial root ganglia causes zona zoster infection. The most recognized feature of zona zoster is the dermatomal distribution of vesicular rashes. In the present case report, we state an unusual presentation of sacral zona zoster with urinary retention, erectile dysfunction and meningitis. Copyright 2009 S. Karger AG, Basel.

  6. Cancer immunotherapy without frontiers: 2nd Annual Immuno-Oncology Meeting of the Centro de Investigación de Cancer en Sonora (CICS), Ciudad Obregón, Sonora México, Dec 2-4, 2016.

    PubMed

    Gallardo-Rincón, Dolores; Marquez, Juan Pablo; Celis, Esteban

    2017-09-01

    This meeting in immuno-oncology brought together clinicians and scientists from United States, Canada, and México with the goal of breaking down international walls and establishing new collaborations.

  7. Instituto Nacional de Electrification, Guatemala Load Dispatch Center and Global Communications Center. Feasibility report (Instituto Nacional de Electrificacion, Guatemala Centro Nacional de Despacho de Carga y Sistema Global de Comunicaciones). Export trade information

    SciTech Connect

    Not Available

    1985-07-01

    The report presents the results of a feasibility study for the National Load Dispatch Center and Global Communications System Project in Guatemala. The project consists of a communication system which will provide Institute Nacional de Electrificacion (INDE) operations personnel direct voice access to all major power system facilities. In addition, a modern computer based load dispatch center has been configured on a secure and reliable basis to provide automatic generation control of all major interconnected generating plants within Guatemala.

  8. Mexico: Centro de Documentacion e Informacion Educativa. Seminario de Documentacion e Informacion Pedagogica en America Latina (The Mexican Center for Educational Documentation and Information. Working Paper for the Seminar on Educational Documentation and Information in Latin America).

    ERIC Educational Resources Information Center

    Secretaria de Educacion Publica, Mexico City (Mexico). Centro de Documentacion e Informacion Educativa.

    This document describes the Center for Documentation and Educational Information in Mexico City. Background information on similar services in Mexico is provided and a detailed description of the Center is presented, covering organization, objectives, administration, users, activities, services, and publications. (VM)

  9. Impact of the implementation of the Infarction Code strategy in patients with acute myocardial infarction diagnosis in the Hospital de Cardiología of the Centro Médico Nacional Siglo XXI

    PubMed

    Sánchez, Gabriela Borrayo; Covarrubias, Héctor Álvarez; Rodríguez, Gilberto Pérez; Uribe, Efraín Arizmendi; Ramírez-Arias, Erick; Peralta, Martín Rosas; Dávila, Jesús Arriaga

    2017-01-01

    To evaluate the impact of the implementation of the Infarction Code strategy in patients with acute myocardial infarction diagnosis. Consecutive patients with ST-elevation acute myocardial infarction ≤12 hours of evolution, were included in the infarction code strategy, before (Group I) and after (Group II). Times of medical attention and major cardiovascular events during hospitalization were analyzed. 1227 patients were included, 919 men (75%) and 308 women (25%) with an average age of 62 ± 11 years. Among Group I and Group II, percutaneous coronary intervention reperfusion therapy changed (16.6% to 42.6%), fibrinolytic therapy (39.3% to 25%), and patients who did not receive any form of reperfusion therapy (44% to 32.6%; p < 0.0001). Times of medical attention decreased significantly (door-to-needle time decreased from 92 to 72 minutes, p = 0.004; door-to-balloon time decreased from 140 to 92 minutes, p < 0.0001). Kidney failure (24.6% vs. 17.9%; p = 0.006), major complications (35.3% to 29.3%), and death (21% vs. 12%; odds ratio: 0.52; 95% confidence interval: 0.38-0.71; p = 0.004). also decreased. The Infarction Code strategy improved treatment, times of medical attention and decreased complications and death in these patients.

  10. El Instituto de Nutrición de Centro América y Panamá: Servicios de Información Científica en Una Región en Vía de Desarrollo *

    PubMed Central

    Flores, Raquel; Corado, Adelina; Girón, Arturo

    1964-01-01

    Pioneering scientific information in underdeveloped areas poses several problems, such as (1) lack of understanding on the part of key functionaries as to the why, what, and when of information services; (2) lack of trained personnel to assume the responsibility for such services; (3) lack of economic resources; and (4) lack of information pertaining to available bibliographical sources in the particular area or region. The Bibliographic and Documentation Service of the Institute of Nutrition of Central America and Panama (INCAP) provides, in addition to the customary services, technical assistance for the organization or reorganization of biomedical libraries and makes INCAP library materials available to member countries, either by loan or photocopy. Although the program was designed for the member countries, INCAP tries to make its services available to other Latin American nations, Africa, and Asia, where similar nutritional problems are being studied. PMID:14119292

  11. [Indicators, variables and obstacles to the life cycle of scientific and technical information in health. Experiences of the Centro Nacional de Información y Documentación en Salud in Mexico].

    PubMed

    Macías-Chapula, C A

    1987-01-01

    Indicators, variables and barriers to scientific and technical health information have been detected and described by CENIDS in Mexico within the life cycle of the information production model, covering the phases of: a) generation; b) institutionalization; c) maintenance/development; and d) distribution/dissemination. Emphasis is placed on the important role that play social, economic, demographic, and political factors of each developing country to conform their ideal information life cycle. After an analysis of the existing Mexican situation, and a short description of CENIDS' structure, organization, functions and services, CENIDS' short-term and mid-term projects, and suggestions for further research lines to improve the current situation in Mexico are also given.

  12. Attivita del Centro di Linguistica Applicata nel campo della lingua e della cultura italiana all'estero (Activities of the Italian Center for Applied Linguistics concerning Italian Language and Culture Abroad).

    ERIC Educational Resources Information Center

    Boriosi Katerinov, Maria Clotilde

    1979-01-01

    Outlines the activities of a recently created section of the Italian Center for Applied Linguistics (CILA), dealing with "Teaching Italian Abroad." Describes these activities as encompassing four areas: research, teaching methodology, consultation, teachers' training and bibliographical information. Lists statistical surveys, research papers, and…

  13. Puerto Rico, humedales [Puerto Rico, wetlands

    USGS Publications Warehouse

    Adams, D. Briane; Hefner, John M.; Dopazo, Teresa

    1999-01-01

    La isla de Puerto Rico, localizada al noreste del Mar Caribe y sus islas principales, Vieques, Culebra e Isla de Mona, poseen humedales en abundancia . El clima subtropical, la lluvia abundante y las complejas formas topográficas y geológicas de estas islas dan origen a los humedales, que varían desde los raros e inusuales bosques cubiertos por nubes en las tierras altas, hasta los extensos manglares, yerbas marinas y arrecifes de coral a lo largo de las costas Norte y Sur. Sin embargo, los humedales en Puerto Rico han disminuido en los últimos siglos como resultado del aumento en el desarrollo agrícola, poblacional y turístico. Algunos tipos de humedales como los bosques de palo de pollo (Pterocarpus officinalis) se han reducido a sólo unos pocos remanentes (figura 1).Biológicamente hablando, los humedales de las islas están entre las áreas más productivas. Los humedales asociados con el bosque pluvial en las tierras altas del interior de Puerto Rico contienen varias plantas raras y especies de animales que no se encuentran en otras partes de la Isla. El agua de escorrentía proveniente de los humedales en las partes altas de la Isla proveen una fuente de agua que utilizan varias ciudades para abasto público. Los humedales costeros como los mangles, los colchones de yerbas marinas y los arrecifes de coral proveen áreas para la reproducción y crianza de varios peces, crustáceos y otras especies en la cadena alimenticia (López y otros, 1988). De esta manera, los humedales costeros contribuyen a la productividad biológica de las aguas llanas del mar alrededor de las islas . Los humedales también estabilizan las costas atrapando y reteniendo sedimentos no consolidados y amortiguan la acción de las olas y de las tormentas que tienen el potencial de causar daños en la zona.El valor de los humedales de Puerto Rico para la vida silvestre está muy bien documentado . Por ejemplo, las salinas de Cabo Rojo, en la costa suroeste, proveen áreas para el

  14. Self-perception of oral health in older adults from an urban population in Lisbon, Portugal.

    PubMed

    Carvalho, Catarina; Manso, Ana Cristina; Escoval, Ana; Salvado, Francisco; Nunes, Carla

    2016-08-22

    To analyze if the self-perception of oral health in the urban context is associated with sociodemographic factors that interfere in the life quality of oral health. Cross-sectional study with convenience sample of older individuals (65 years old or more) enrolled in the Agrupamento de Centros de Saúde de Lisboa Norte (ACES Lisboa Norte - Health Centers Groupings North Lisbon). The self-perception of oral health and associated life quality was evaluated by the Geriatric Oral Health Assessment Index and the individuals were classified according to sociodemographic characteristics. The internal consistency of the questionnaire was evaluated by Cronbach's alpha (α). Later, we used binary logistic regression models to characterize the factors associated with the self-perception of oral health, considering the sociodemographic variables and the older adults' clinical conditions of oral health and establishing the crude and adjusted (to age) odds ratios and their 90% confidence intervals. A total of 369 older adults participated in this study, with an average age of 74.2 years (SD = 6.75); 62.9% were female. On average, the index was moderated, with tendency to be high: 32.9 (SD = 3.6; 12-36 interval). The Cronbach's alpha was high: 0.805. Age, marital status, and the last dental appointment were the factors significantly associated with self-perception of oral health. The study shows that these individuals have a moderate, with tendency to high, self-perception of oral health. The self-perception of oral health assessment allowed us to identify the main associated sociodemographic factors. This instrument can help guiding planning strategies and oral health promotion directed toward a better life quality for this population group. Analisar se a autopercepção de saúde bucal em contexto urbano está associada aos factores sociodemográficos que interferem na qualidade de vida da saúde bucal. Estudo transversal com amostra de conveniência de indivíduos idosos (65

  15. 76 FR 28181 - Approval and Promulgation of Air Quality Implementation Plans; New Mexico; Sunland Park Section...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-16

    ... Santa Teresa are located along the border region of New Mexico and are adjacent to El Paso, Texas, and Ciudad Juarez, Mexico, or what is commonly referred to as the Paso del Norte Airshed. New Mexico... due to airshed contributions from Mexico and Texas. Air quality within the Paso del Norte Airshed has...

  16. 60. C.J.T., photographer December 23, 1955 KLAMATH RIVER BRIDGE, DEL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    60. C.J.T., photographer December 23, 1955 KLAMATH RIVER BRIDGE, DEL NORTE COUNTY, SECTION A, HIGHWAY 1. DN-1-A #538, KLAMATH RIVER BR. FROM SO. END, 12/23/55, C.J.T. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  17. Paleoseismic investigations at the Cal thrust fault, Mendoza, Argentina

    NASA Astrophysics Data System (ADS)

    Salomon, Eric; Schmidt, Silke; Hetzel, Ralf; Mingorance, Francisco

    2010-05-01

    determine the recurrence interval of these seismic events and the long-term slip rate of the fault is in progress. References Mingorance, F. (2006): Morfometría de la escarpa de falla historica identificada al norte del cerro La Cal, zona de falla La Cal, Mendoza. Revista de la Asociación Geológica Argentina, 61 (4), 620-638.

  18. Low-dose, Chronic Exposure to Silver Nanoparticles Causes Mild Mitochondrial Alterations in the Liver of Sprague-Dawley Rat

    DTIC Science & Technology

    2014-05-10

    of Sprague-Dawley rat CARLOS PALMEIRA CENTRO DE NEUROCIENCIAS E BIOLOGIA CELULAR (CNC) DEPARTAMENTO DE ZOOLOGIA COIMBRA 3000 - 329...CENTRO DE NEUROCIENCIAS E BIOLOGIA CELULAR (CNC) DEPARTAMENTO DE ZOOLOGIA COIMBRA 3000 - 329 PORTUGAL 8. PERFORMING ORGANIZATION REPORT NUMBER

  19. Characterisation of gas and particle emissions from wildfires =

    NASA Astrophysics Data System (ADS)

    Vicente, Ana Margarida Proenca

    Os incendios florestais sao uma importante fonte de emissao de compostos gasosos e de aerossois. Em Portugal, onde a maioria dos incendios ocorre no norte e centro do pais, os incendios destroem todos os anos milhares de hectares, com importantes perdas em termos economicos, de vidas humanas e qualidade ambiental. As emissoes podem alterar consideravelmente a quimica da atmosfera, degradar a qualidade do ar e alterar o clima. Contudo, a informacao sobre as carateristicas das emissoes dos incendios florestais nos paises do Mediterrâneo e limitada. Tanto a nivel nacional como internacional, existe um interesse crescente na elaboracao de inventarios de emissoes e de regulamentos sobre as emissoes de carbono para a atmosfera. Do ponto de vista atmosferico da monitorizacao atmosferica, os incendios sao considerados um desafio, dada a sua variabilidade temporal e espacial, sendo de esperar um aumento da sua frequencia, dimensao e severidade, e tambem porque as estimativas de emissoes dependem das carateristicas dos biocombustiveis e da fase de combustao. O objetivo deste estudo foi quantificar e caraterizar as emissoes de gases e aerossois de alguns dos mais representativos incendios florestais que ocorreram no centro de Portugal nos veroes de 2009 e de 2010. Efetuou-se a colheita de amostras de gases e de duas fracoes de particulas (PM2.5 e PM2.5-10) nas plumas de fumo em sacos Tedlar e em filtros de quartzo acoplados a um amostrador de elevado volume, respetivamente. Os hidrocarbonetos totais (THC) e oxidos de carbono (CO e CO2) nas amostras gasosas foram analisados em instrumentos automaticos de ionizacao de chama e detetores nao dispersivos de infravermelhos, respetivamente. Para algumas amostras, foram tambem quantificados alguns compostos de carbonilo apos reamostragem do gas dos sacos Tedlar em cartuchos de silica gel revestidos com 2,4-dinitrofenilhidrazina (DNPH), seguida de analise por cromatografia liquida de alta resolucao. Nas particulas, analisou-se o

  20. Hydrochemical tracers in the middle Rio Grande Basin, USA: 1. Conceptualization of groundwater flow

    NASA Astrophysics Data System (ADS)

    Plummer, L. Niel; Bexfield, Laura M.; Anderholm, Scott K.; Sanford, Ward E.; Busenberg, Eurybiades

    zones hydro-chimiques reflète principalement la distribution moderne des charges hydrauliques mais est incohérente avec une dépression dans le niveau d'eau dans la partie centre-ouest du bassin, ce qui indique que cette dépression est un élément transitoire du système aquifère plutôt qu'un élément à long terme. Les âges radiocarbones ajustés aux réactions géochimiques et aux processus de mélange et d'évapotranspiration/dilution qui ont lieu dans l'aquifère sont presque identiques aux âges non ajustés et varient de la période moderne jusqu'à 30 ka. Les gradients d'âge établis à partir des nids de piézomètres s'étendent de 0.1 à 2 a cm-1 et suggèrent un taux de recharge d'environ 3 cm a-1 le long du front des montagnes à l'est et pour l'infiltration provenant du Rio Grande près d'Albuquerque. Il y a eu substantiellement moins de recharge le long du front des montagnes à l'est, au nord et au sud d'Albuquerque. Se utilizaron datos químicos e isotópicos de agua subterránea a lo largo de la cuenca central del río Grande, Nuevo México, EEUU, para identificar y mapear el flujo de agua subterránea de 12 fuentes de agua a la cuenca para evaluar edades por medio de radio carbon y para refinar el modelo conceptual del sistema acuífero del Grupo Santa Fé. Se puede establecer zonas hidrotérmicas que representan el flujo de agua subterránea a lo largo de miles a miles de decenas de años en grandes distancias a través del sistema acuífero principalmente siliclástico. Las ubicaciones de las zonas hidroquímicas mayormente reflejan la distribucion de la cabeza hidráulica pre-desarollo moderna pero son inconsistentes con una depresión en los niveles de agua pre-desarollo en la zona central oeste de la cuenca. Esto indica que esta depresión es un rasgo transitorio y no un rasgo de largo plazo del sistema acuífero. Las edades de radio carbon ajustadas para los procesos de reaciones geoquímicas, de mezclado y de evapotranspiraci

  1. Geology and hydrogeology of the Dammam Formation in Kuwait

    NASA Astrophysics Data System (ADS)

    Al-Awadi, E.; Mukhopadhyay, A.; Al-Senafy, M. N.

    différence de niveau piézométrique est observée entre la formation de Damman et le groupe de Koweit qui la recouvre en discordance; cette différence est due à la présence d'un niveau imperméable qui la maintient captive. Resumen La Formación Damman, del Eoceno Medio, es uno de los mayores acuíferos de agua salobre aprovechable en Kuwait. Además de una zona de paleokarst en la parte superior, la Formación Damman en Kuwait consiste en 150-200m de caliza dolomitizada, que se subdivide en tres zonas en función de la litología y la biofacies. La parte superior está formada por dolomicrita yesífera friable y dolomita. La parte central es básicamente biomicrita laminada y biodolomicrita. La inferior es caliza nummulítica, con intercalaciones de pizarra en la base. Los marcadores geofísicos reflejan claramente estas subdivisiones. Los análisis de testigos revelan que la parte superior es la más porosa y permeable de las tres unidades. Esto queda confirmado con la distribución de zonas de circulación perdidas. La calidad del agua en el acuífero se deteriora hacia el norte y el este. Sobre la Formación Damman, y de manera no conforme, suprayace otra formación, que se conoce como Grupo Kuwaití. Existe una diferencia de niveles piezométricos entre ambas formaciones, la cual se mantiene por la presencia de una capa semiconfinante.

  2. Morphology and ultrastructure of the adrenal gland in Bactrian camels (Camelus bactrianus).

    PubMed

    Ye, Wen-Ling; Wang, Feng-Ling; Wang, Hong-Ju; Wang, Jian-Lin

    2017-04-01

    In the present study, we examined the morphological features of the adrenal gland in Bactrian camel by means of digital anatomy, light and electron microscopy. Our findings testified that the gland was divided into three parts, capsule, cortex and medulla from outside to inside as other mammals, and the cortex itself was further distinguished into four zones: zona glomerulosa, zona intermedia, zona fasciculate and zona reticularis. Notably, the zona intermedia could be seen clearly in the glands from females and castrated males, whereas it was not morphologically clear in male. There was a great deal of lipid droplets in the zona fasciculate, while it was fewer in the zona glomerulosa and zona reticularis. The cytoplasm of adrenocortical cell contained rich mitochondria and endoplasmic reticulum. The adrenal medulla was well-developed with two separations of external and internal zones. The most obvious histological property of adrenal medulla cells were that they contained a huge number of electron-dense granules enveloped by the membrane, and so medulla cells could be divided into norepinephrine cells and epinephrine cells. Moreover, the cortical cuffs were frequently present in adrenal gland. Results of this study provides a theoretical basis necessary for ongoing investigations on Bactrian camels and their good adaptability in arid and semi-arid circumstances. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Mapa de Vulnerabilidad en Honduras

    NASA Astrophysics Data System (ADS)

    Sánchez, A. A.

    2013-05-01

    Dado que Honduras geográficamente se encuentra en una zona que esta expuesta a diferentes fenómenos sísmicos y climatológicos. Investigamos cuales son las zonas mas propensas a desastres naturales, la vulnerabilidad y el factor de resiliencia de la población de acuerdo a diferentes parámetros de estudio.

  4. Chemotherapeutic Studies on Schistosomiasis and Clinical, Epidemiological and Immunological Studies on Malaria in Amazonas, Brazil, along the Ituxi River.

    DTIC Science & Technology

    1982-09-01

    enfermedades infecciosas en zonas colindantes con la Carretera Transamazonica en Brasil. II. Epidemiologia de las infecciones por arbovirus. Bol. Of...F.P. 1981. Programa multidisciplinario de vigilancia de las enfermedades , infecciosas en zonas colindantes con la Carretera Transamazonica en Brasil

  5. Heterogeneous levels of oxidative phosphorylation enzymes in rat adrenal glands.

    PubMed

    Ogawa, Koichi; Harada, Keita; Endo, Yutaka; Sagawa, Sueko; Inoue, Masumi

    2011-01-01

    Mitochondria are organelles that produce ATP and reactive oxygen species, which are thought to be responsible for a decline in physiological function with aging. In this study, we morphologically and biochemically examined mitochondria in the rat adrenal gland. Immunohistochemistry showed that the rank order for intensity of immunolabelling for complex IV was zona reticularis > zona fasciculata > adrenal medulla, whereas for complex V α and β subunits, it was zona fasciculata > zona reticularis and adrenal medulla. The immunolabelling for complex I was homogeneous in the adrenal gland. The difference in immunolabelling between complexes I and IV indicates that the ratio of levels of complex I to that of complex IV in the zona reticularis was smaller than that in the zona fasciculata and the adrenal medulla. Electron microscopy revealed that aging rats had zona reticularis cells with many lysosomes and irregular nuclei. The result suggests that the level of proteins involved in oxidative phosphorylation is coordinated within the complex, but differs between the complexes. This might be responsible for degeneration of zona reticularis cells with aging. Copyright © 2009 Elsevier GmbH. All rights reserved.

  6. Journal of Transportation and Statistics : special issue on the Northridge earthquake

    DOT National Transportation Integrated Search

    1998-09-01

    This is special issue on the Northridge Earthquake. Contents: Impacts of the Northridge Earthquake on Transit and Highway Use by Genevieve Giuliano; Transport Related Impacts on the Northridge Earthquake by Peter Gordon; Goods Movement After the Nort...

  7. Evaluation of continuity detail for precast prestressed girders : research project capsule.

    DOT National Transportation Integrated Search

    2008-03-01

    The construction of a new bridge : crossing the Mississippi River north of : Baton Rouge is currently underway. The : project, named the John James : Audubon Bridge, is a true landmark. Its : main span will be the longest cablestayed : bridge in Nort...

  8. 27. AVENUE OF THE GIANTS SIGN AT NORTH END OF ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    27. AVENUE OF THE GIANTS SIGN AT NORTH END OF ROAD. PEPPERWOOD, HUMBOLDT COUNTY, CALIFORNIA. LOOKING S. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  9. 2. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  10. 67. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    67. WILLITS SIGN. GATEWAY TO THE REDWOODS. WILLITS, MENDOCINO COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  11. 37. BRIDGE 115, SMITH RIVER MIDDLE FORK OREGON STATE HIGHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    37. BRIDGE 1-15, SMITH RIVER MIDDLE FORK OREGON STATE HIGHWAY 199. JOSEPHINE COUNTY, OREGON. LOOKING SSW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  12. 40. CAVEMAN BRIDGE, ROGUE RIVER, OREGON STATE HIGHWAY 199. GRANTS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    40. CAVEMAN BRIDGE, ROGUE RIVER, OREGON STATE HIGHWAY 199. GRANTS PASS, JOSEPHINE COUNTY, OREGON. LOOKING S. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  13. 39. CAVEMAN BRIDGE, ROGUE RIVER, OREGON STATE HIGHWAY 199. GRANTS ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    39. CAVEMAN BRIDGE, ROGUE RIVER, OREGON STATE HIGHWAY 199. GRANTS PASS, JOSEPHINE COUNTY, OREGON. LOOKING SW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  14. 36. MYRTLE CREEK BRIDGE, OREGON STATE HIGHWAY 199, AT END ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    36. MYRTLE CREEK BRIDGE, OREGON STATE HIGHWAY 199, AT END OF STOUT GROVE ROAD. JOSEPHINE COUNTY, OREGON LOOKING WNW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  15. 71. MYRTLE CREED BRIDGE, OREGON STATE HIGHWAY 199, AT END ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    71. MYRTLE CREED BRIDGE, OREGON STATE HIGHWAY 199, AT END OF STOUT GROVE ROAD. JOSEPHINE COUNTY, OREGON. LOOKING WNW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  16. Remediation System Evaluation, Summitville Mine Superfund Site

    EPA Pesticide Factsheets

    The Summitville Mine Superfund Site is located in the southeastern portion of the San Juan Mountains,in Rio Grande County, approximately 60 miles west of Alamosa, Colorado and 10 to 15 miles south ofDel Norte, Colorado.

  17. 22. EXTERIOR DETAIL VIEW OF SOUTHEAST CORNER OF BRIDGE, 1932 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. EXTERIOR DETAIL VIEW OF SOUTHEAST CORNER OF BRIDGE, 1932 SECTION, SOUTH BANK, LOOKING NORTHEAST TO SOUTHWEST - Rowdy Creek Bridge, Spanning Rowdy Creek at Fred Haight Drive, Smith River, Del Norte County, CA

  18. 5. TUNNEL TREE AT DRIVETHROUGHTREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. TUNNEL TREE AT DRIVE-THROUGH-TREE PARK. LEGGETT, HUMBOLDT COUNTY, CALIFORNIA. LOOKING NE. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  19. Incorporating weather impacts in traffic estimation and prediction systems (TREPS)

    DOT National Transportation Integrated Search

    2009-09-01

    This document provides quantitative benefits of using Intelligent Transportation Systems in highway construction and maintenance work zones. The technical report covers case study sites in the District of Columbia, Texas, Michigan, Arkansas, and Nort...

  20. Evaluation of LIDAR for landslide mapping.

    DOT National Transportation Integrated Search

    2006-06-01

    The Caltrans GeoResearch Group, in collaboration with the Department of Conservation, successfully : used LIDAR technology to map landslides along two heavily forested highway corridors in Humboldt : and Del Norte Counties. LIDAR (Light Detection ...

  1. 52. G.E.H. photographer August 1930 1HUM1B #59, OILING OPERATIONS, GEH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    52. G.E.H. photographer August 1930 1-HUM-1-B #59, OILING OPERATIONS, GEH 8-30. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  2. 48. Photographer unknown February 1925 MENDOCINO COUNTY, SECTION K, HIGHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    48. Photographer unknown February 1925 MENDOCINO COUNTY, SECTION K, HIGHWAY 1. 1-MEN-1-K #39, LOG GUARD RAIL, 2-25. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  3. 1. HEALDSBURG BRIDGE, OLD HIGHWAY 101, ACROSS THE RUSSIAN RIVER. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. HEALDSBURG BRIDGE, OLD HIGHWAY 101, ACROSS THE RUSSIAN RIVER. HEALDSBURG, MEDDOCINO COUNTY, CALIFORNIA. LOOKING NW. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  4. Estimating pothole wetland connectivity to Pipestem Creek, North Dakota: an isotopic approach

    EPA Science Inventory

    Understanding hydrologic connectivity between wetlands and perennial streams is critical to understanding how reliant stream flow is on wetlands within their watershed. We used the isotopic evaporation signal in water to examine hydrologic connectivity within Pipestem Creek, Nort...

  5. 12. VIEW, LOOKING NORTHWEST, ALONG CENTERLINE FROM NORTH CANTILEVER TOWER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    12. VIEW, LOOKING NORTHWEST, ALONG CENTERLINE FROM NORTH CANTILEVER TOWER TOWARD SOUTH PORTAL. As with previous photo, note vertical displacement in deck. - Smith River Bridge, CA State Highway 199 Spanning Smith River, Crescent City, Del Norte County, CA

  6. Effect of climate change on wind waves generated by anticyclonic cold front intrusions in the Gulf of Mexico

    NASA Astrophysics Data System (ADS)

    Appendini, Christian M.; Hernández-Lasheras, Jaime; Meza-Padilla, Rafael; Kurczyn, Jorge A.

    2018-01-01

    Anticyclonic cold surges entering the Gulf of Mexico (Nortes) generate ocean waves that disrupt maritime activities. Norte derived waves are less energetic than the devastating waves from tropical cyclones, but more frequent ( 22 events/year) and with larger spatial influence. Despite their importance, few studies characterize Nortes derived waves and assess the effects of climate change on their occurrence. This study presents a method to identify and characterize Nortes with relation to their derived waves in the Gulf of Mexico. We based the identification of Nortes on synoptic measurements of pressure differences between Yucatan and Texas and wind speed at different buoy locations in the Gulf of Mexico. Subsequently, we identified the events in the CFSR reanalysis (present climate) and the CNRM-M5 model for the present climate and the RCP 8.5 scenario. We then forced a wave model to characterize the wave power generated by each event, followed by a principal component analysis and classification by k-means clustering analysis. Five different Nortes types were identified, each one representing a characteristic intensity and area of influence of the Norte driven waves. Finally, we estimated the occurrence of each Norte type for the present and future climates, where the CNRM-M5 results indicate that the high-intensity events will be less frequent in a warming climate, while mild events will become more frequent. The consequences of such changes may provide relief for maritime and coastal operations because of reduced downtimes. This result is particularly relevant for the operational design of coastal and marine facilities.

  7. Mitomycin C plus vindesine plus etoposide (MEV) versus mitomycin C plus vindesine plus cisplatin (MVP) in stage IV non-small-cell lung cancer: A phase III multicentre randomised trial. The "Gruppo Oncologico Centro-Sud-Isole' (G.O.C.S.I.).

    PubMed

    Gridelli, C; Perrone, F; Palmeri, S; D'Aprile, M; Cognetti, F; Rossi, A; Gebbia, V; Pepe, R; Veltri, E; Airoma, G; Russo, A; Incoronato, P; Scinto, A F; Palazzolo, G; Natali, M; Leonardi, V; Gallo, C; De Placido, S; Bianco, A R

    1996-10-01

    To compare mitomycin C plus vindesine plus etoposide (MEV) vs. mitomycin C plus vindesine plus cisplatin (MVP) in the treatment of stage IV non-small-cell lung cancer. 204 patients were entered in a phase III multicentre randomised trial from June 1990 to December 1994 and stratified according to the ECOG performance status (0-1 vs. 2). MVP was given in the following dosages: mitomycin C 8 mg/m2+vindesine 3 mg/m2+cisplatin 100 mg/m2 i.v. day 1 and vindesine 3 mg/m2 i.v. day 8 with cycles repeated every 4 weeks. MEV was given in the following dosages: mitomycin C 8 mg/m2+vindesine 3 mg/ m2 i.v. day 1 and etoposide 100 mg/m2 i.v. days 1 to 3 with cycles repeated every 3 weeks. For both treatments a maximum of 6 cycles was planned. Response and toxicity were evaluated according to WHO. Subjective responses were assessed by numerical scales. Analyses were made on the basis of intent to treat. The objective response rate was 21.4% (1 CR + 21 PR among 103 patients) in the MEV and 28.7% (1 CR + 28 PR among 101 patients) in the MVP arm (P = 0.48). Symptoms were similar in the two arms. 196 patients progressed and 182 died. The median times to progression were 10 weeks (95% CI 9-12) and 12 weeks (95% CI 10-15) and median survivals were 29 weeks (95% CI 25-36) and 28 weeks (95% CI 25-35) in the MEV and MVP arms, respectively. The relative risks of progressing and of dying were 0.89 (95% CL 0.66-1.20) and 0.96 (95% CL 0.71-1.30), respectively, for patients receiving MVP as compared with those receiving MEV at multivariate analysis adjusted by sex, age, histologic type, number of metastatic sites, performance status at entry, and centre. In the present study, no significant differences were observed in response rate, survival or palliation of symptoms between the MEV and MVP regimens, while toxicity was significantly more frequent and severe with MVP. Thus, MEV should be considered a reasonable alternative to the MVP regimen in the treatment of stage IV NSCLC.

  8. [Epidemiologic aspects of the dialysis treatment in Grande São Paulo. Comissão Regional de Nefrologia da Secretaria da Saúde de São Paulo and Centro de Informática da Escola Paulista de Medicina].

    PubMed

    Sesso, R; Anção, M S; Madeira, S A

    1994-01-01

    Epidemiological data about the treatment of patients with end-stage renal disease in the Great Sao Paulo, Brazil, are presented. Patients on dialysis in the city of Sao Paulo and surroundings, distributed in 15 Regional Offices of Health (ERSAs), during 1991, were studied. Data were collected by the Secretary of Health of the State of Sao Paulo. There was an increase of 18.6% in the number of alive patients on dialysis from January 1 to December 31 (n = 2,425 to 2,875). Patients were treated in 40 dialysis centers, of which 24 were located in the ERSAs 1, 2 and 3. Depending on the ERSA, a percentage of patients varying from 37% to 88% did not live in the same region where they received treatment. At the end of the year, 79% of the patients were on hemodialysis, 15% on continuous ambulatory peritoneal dialysis and 6% on intermittent peritoneal dialysis. The diagnoses more frequently reported of primary disease were non-determined, glomerulonephritis, hypertension and diabetes (36%, 27%, 17% and 8%, respectively). New cases (1,483) initiated dialysis during the year, corresponding to an incidence rate of 83 patients per million population (pmp). The prevalence of patients on dialysis was 148 pmp. The annual fatality rate was 17.2% (range in the ERSAs: 12.0-3.5). The actuarial one year survival for the patients who started treatment in 1991 was 80.2%. 246 patients received transplantation, corresponding to 14 patients pmp. Dialysis treatment provided in the Great Sao Paulo is satisfactory.(ABSTRACT TRUNCATED AT 250 WORDS)

  9. Students and Teachers' Reasons for Using the First Language within the Foreign Language Classroom (French and English) in Central Mexico (Razones de alumnos y maestros sobre el uso de la primera lengua en el salón de lenguas extranjeras (francés e inglés) en el centro de México)

    ERIC Educational Resources Information Center

    Mora Pablo, Irasema; Lengeling, M. Martha; Rubio Zenil, Buenaventura; Crawford, Troy; Goodwin, Douglas

    2011-01-01

    The present study explores the use of the first language in a context of foreign language teaching. This qualitative research presents the classroom practice and points of view of French and English teachers and students within a public educational institute in central Mexico using the techniques of questionnaires and semi-structured interviews.…

  10. [Speech by Oscar Julian Bardeci, director of the Centro Latinoamericano de Demografia (CELADE), at the Latinamerican Regional Meeting prior to the International Conference on Population in recognition of the Second Meeting on Population by the Committee of Upper-Level Government Experts (CEGAN), Havana, Cuba, November 16-19, 1983].

    PubMed

    Bardeci, O J

    1983-12-01

    This work examines the relationship between population growth and economic development in Latin America and assesses progress in the 10 years since the Bucharest World Population Conference. The Latin American population increased from about 159 million in 1950 to 275 million in 1970 and around 325 million in 1980. The rate of growth reached a maximum of 2.8%/year in the early 1970s and has now declined to about 2.3%/year. The regional growth rate is a product of population dynamics that differ greatly in individual countries. Crude birth rates declined in every country of Latin America between 1975-80, but still exceeded 40/1000 in 1980-85 in Bolivia, Ecuador, El Salvador, Haiti, Honduras, and Nicaragua. Current fertility is the result of the different trajectories of the demographic transition in different countries. While fertility in Argentina, Cuba, and Uruguay underwent a slow but sustained decline that began prior to 1960, other countries including Colombia, Costa Rica, Chile, and Venezuela began an accelerated fertility decline in the 1960s that diffused rapidly through all age and social groups. Other countries have still not entered a definite phase of fertility decline. Mortality rates have declined appreciably in Latin America in the past few decades although they remain high in some countries. After the end of World War II and until the mid-1970s, most countries of the region experienced rapid economic growth coupled with profound changes in the productive structure. The industrial labor force grew in almost all countries along with urbanization, the decline of agricultural employment, and the increase of the tertiary sector. These and other important economic advances through the mid-1970s occurred despite rapid population growth, and the beginning of the fertility decline coincided with slowing economic growth that saw negative rates in 1981-82. Various studies have shown that not all population sectors were incorporated in the process of economic and social development. The proportion of the population in active age groups has increased dramatically, but no corresponding increase in employment opportunities has occurred. The informal sector has absorbed most of the excess growth, a trend reflected in the exclusion of a large segment of the population from the fruits of economic progress. Population growth and increased life expectancy have created demands for more retirement benefits, health and educational services, transportation, recreation, and housing. Nevertheless, it cannot be concluded that rapid population growth necessarily has negative consequences for economic development or social welfare. There are indications that the problem of poverty in Latin America could be solved if the political will to do so existed; obstacles to overcoming poverty are not primarily material or population-related. Urbanization, settlement of marginal areas, and relative decline of agricultural areas are 3 trends in spatial distribution of varying intensity and repercussions in different countries.

  11. Para Candidatos en Programas de Centros de Cuidado y Educacion Infantil con Ninos de Edad Pre-escolar: Asociado en Desarrollo Infantil Sistema de Evaluacion y Normas de Competencia CDA (Preschool Caregivers in Center-Based Programs: The Child Development Associate Assessment System and Competency Standards).

    ERIC Educational Resources Information Center

    Council for Early Childhood Professional Recognition, Washington, DC.

    This Spanish-language booklet outlines the requirements of the Child Development Associate (CDA) credential for preschool teachers or caregivers who work in center-based preschool day care programs. Part 1 provides an overview of the CDA credentialing system and the various options, settings, standards, and stages of the CDA assessment system.…

  12. Para Candidatos en Programas de Centros de Cuidado y Educacion Infantil con Bebes y "Toddlers": Asociado en Desarrollo Infantil Sistema de Evaluacion y Normas de Competencia CDA (Infant/Toddler Caregivers in Center-Based Programs: The Child Development Associate Assessment System and Competency Standards).

    ERIC Educational Resources Information Center

    Council for Early Childhood Professional Recognition, Washington, DC.

    This Spanish-language booklet outlines the requirements of the Child Development Associate (CDA) credential for caregivers working in center-based infant and toddler day care programs. Part 1 provides an overview of the CDA credentialing system and the various options, settings, standards, and stages of the CDA assessment system. Part 2 explains…

  13. Negative pressure of the environmental air in the cleaning area of the materials and sterilization center: a systematic review.

    PubMed

    Ciofi-Silva, Caroline Lopes; Hansen, Lisbeth Lima; Almeida, Alda Graciele Claudio Dos Santos; Kawagoe, Julia Yaeko; Padoveze, Maria Clara; Graziano, Kazuko Uchikawa

    2016-09-01

    , porém não foram encontrados artigos científicos sobre o impacto dessa intervenção. Os quatro artigos incluídos trataram dos aerossóis formados após uso de lavadora ultrassônica (aumento da contaminação principalmente durante o uso) e do jato de água pressurizado (formação de aerossóis menores que 5µm). Em um estudo foram avaliados aerossóis formados a partir de torneira de água quente contaminada com Legionella pneumophila. há evidências sobre formação de aerossóis durante atividades de limpeza em CME. Estudos sobre doenças ocupacionais de origem respiratória dos trabalhadores que atuam em CME devem ser realizados. examinar la evidencia científica sobre los aerosoles generados durante las actividades de limpieza de productos de salud en el Centro de Materiales y Esterilización (CME) y el impacto de la presión negativa del aire ambiente en la zona de limpieza para controlar la dispersión de aerosoles a las zonas adyacentes. para esta revisión sistemática de literatura se llevaron a cabo: búsqueda de directrices, manuales o normas técnicas nacionales e internacionales impartidas por expertos; búsqueda en las bases de datos PUBMED, SCOPUS, Cinahl y Web of Science; y búsqueda manual de artículos científicos. Los cinco documentos técnicos analizados recomiendan que el área de limpieza del CME tenga un diferencial negativo de presión en el aire ambiente, aunque no se han encontrado artículos científicos sobre el impacto de esta intervención. Los cuatro artículos incluidos fueron sobre los aerosoles formados después del uso de la lavadora ultrasónica (aumento de la contaminación, especialmente durante el uso) y chorro de agua a presión (formación de aerosoles menores a 5µm). De esta forma, en un solo estudio se evaluaron los aerosoles formados a partir de un grifo de agua caliente contaminada con Legionella pneumophila. hay evidencia de formación de aerosoles durante las actividades de limpieza en CME. Sin embargo, es

  14. The involvement of beta-1,4-Galactosyltransferase and N-Acetylglucosamine residues in fertilization has been lost in the horse.

    PubMed

    Mugnier, Sylvie; Boittin, Stéphane; Douet, Cécile; Monget, Philippe; Magistrini, Michèle; Goudet, Ghylène

    2008-11-14

    In human and rodents, sperm-zona pellucida binding is mediated by a sperm surface Galactosyltransferase that recognizes N-Acetylglucosamine residues on a glycoprotein ZPC. In large domestic mammals, the role of these molecules remains unclear: in bovine, they are involved in sperm-zona pellucida binding, whereas in porcine, they are not necessary. Our aim was to clarify the role of Galactosyltransferase and N-Acetylglucosamine residues in sperm-zona pellucida binding in ungulates. For this purpose, we analyzed the mechanism of sperm-zona pellucida interaction in a third ungulate: the horse, since the Galactosyltransferase and N-Acetylglucosamine residues have been localized on equine gametes. We masked the Galactosyltransferase and N-Acetylglucosamine residues before the co-incubation of gametes. Galactosyltransferase was masked either with an anti-Galactosyltransferase antibody or with the enzyme substrate, UDP Galactose. N-Acetylglucosamine residues were masked either with a purified Galactosyltransferase or with an anti-ZPC antibody. The number of spermatozoa bound to the zona pellucida did not decrease after the masking of Galactosyltransferase or N-Acetylglucosamine. So, these two molecules may not be necessary in the mechanism of in vitro sperm-zona pellucida interaction in the horse. The involvement of Galactosyltransferase and N-Acetylglucosamine residues in sperm-zona pellucida binding may have been lost during evolution in some ungulates, such as porcine and equine species.

  15. Distribution of stable isotopes in arid storms . II. A double-component model of kinematic wave flow and transport

    NASA Astrophysics Data System (ADS)

    Yakirevich, Alexander; Dody, Avraham; Adar, Eilon M.; Borisov, Viacheslav; Geyh, Mebus

    édible par rapport aux observations a été obtenu après un ajustement des paramètres par une méthode d'essais et d'erreurs. L'analyse de sensibilité et l'application du modèle ont ensuite été réalisés. Le modèle est plutôt sensible aux changements des paramètres caractérisant les zones de stockage dans les dépressions. Le modèle rend compte de l'effet de mémoire isotopique dans l'eau dans le stockage des dépressions entre les événements séquentiels de pluie. L'utilisation d'un modèle à double composante d'écoulement et de transport par onde cinématique permet un ajustement qualitatif et quantitatif adapté entre les distributions des δ18O calculées et observées dans l'écoulement de surface. Resumen Un nuevo método matemático basado en un modelo de doble componente de onda cinemática de flujo y transporte permite caracterizar la distribución isotópica dinámica de las tormentas en zonas áridas y la escorrentía. Este modelo describe el transporte y la evolución del δ18O en la lluvia, flujo superficial y escorrentía en una cuenca rocosa de clima árido con detención superficial distribuida uniformemente. El modelo se calibró numéricamente utilizando un conjunto de datos de descarga temporal y de distribución de δ18O para lluvia y escorrentía recogida en una pequeña cuenca rocosa en el Centro de Experimentación de Sede Boker, Israel. Se obtuvo un buen ajuste a los datos tras un ajuste de parámetros mediante prueba y error. Se realizó un análisis de sensibilidad que indicó que el modelo resulta ser bastante sensible a cambios en los parámetros que caracterizan las zonas de baja detención superficial. El modelo también refleja el efecto de la memoria isotópica en el agua de estas zonas de detención entre los distintos periodos de lluvias. El uso de un modelo de doble componente de onda cinemática de flujo y transporte proporciona un buen ajuste cualitativo y cuantitativo entre los datos medidos y calculados de δ18O en la

  16. Transcriptome Pathway Analysis of Pathological and Physiological Aldosterone-Producing Human Tissues.

    PubMed

    Zhou, Junhua; Lam, Brian; Neogi, Sudeshna G; Yeo, Giles S H; Azizan, Elena A B; Brown, Morris J

    2016-12-01

    Primary aldosteronism is present in ≈10% of hypertensives. We previously performed a microarray assay on aldosterone-producing adenomas and their paired zona glomerulosa and fasciculata. Confirmation of top genes validated the study design and functional experiments of zona glomerulosa selective genes established the role of the encoded proteins in aldosterone regulation. In this study, we further analyzed our microarray data using AmiGO 2 for gene ontology enrichment and Ingenuity Pathway Analysis to identify potential biological processes and canonical pathways involved in pathological and physiological aldosterone regulation. Genes differentially regulated in aldosterone-producing adenoma and zona glomerulosa were associated with steroid metabolic processes gene ontology terms. Terms related to the Wnt signaling pathway were enriched in zona glomerulosa only. Ingenuity Pathway Analysis showed "NRF2-mediated oxidative stress response pathway" and "LPS (lipopolysaccharide)/IL-1 (interleukin-1)-mediated inhibition of RXR (retinoid X receptor) function" were affected in both aldosterone-producing adenoma and zona glomerulosa with associated genes having up to 21- and 8-fold differences, respectively. Comparing KCNJ5-mutant aldosterone-producing adenoma, zona glomerulosa, and zona fasciculata samples with wild-type samples, 138, 56, and 59 genes were differentially expressed, respectively (fold-change >2; P<0.05). ACSS3, encoding the enzyme that synthesizes acetyl-CoA, was the top gene upregulated in KCNJ5-mutant aldosterone-producing adenoma compared with wild-type. NEFM, a gene highly upregulated in zona glomerulosa, was upregulated in KCNJ5 wild-type aldosterone-producing adenomas. NR4A2, the transcription factor for aldosterone synthase, was highly expressed in zona fasciculata adjacent to a KCNJ5-mutant aldosterone-producing adenoma. Further interrogation of these genes and pathways could potentially provide further insights into the pathology of primary

  17. Exploration of an alluvial aquifer in Oman by time-domain electromagnetic sounding

    NASA Astrophysics Data System (ADS)

    Young, M. E.; de Bruijn, R. G. M.; Al-Ismaily, A. Salim

    sont grossièrement caractérisés par des différentes gammes de résistivitéélectrique se recouvrant, dépendant surtout de la teneur en argiles et du degré de cimentation. Les résistivités près de la côte diminuent du fait de l'intrusion marine. Ces variations de résistivité ont été cartographiées à partir de 400km de profils électromagnétiques, dans le domaine temporel ; trois zones ont été distinguées dans l'aquifère alluvial. Le biseau salé a aussi été délimité, jusqu'à 10km de la côte. L'épaisseur de la partie saturée des graviers va de 20-160m sur une étendue supérieure à 600km2. Resumen Un tercio de la población de Omán depende de las aguas subterráneas extraídas del aluvial de la Llanura de Batinah, en la costa del Golfo de Omán. Para determinar la profundidad y naturaleza del aluvial, así como los límites del acuífero, se han usado técnicas de exploración geofísica profunda. El basamento y los límites estructurales del aluvial en su contacto con los sedimentos marinos del Terciario y la ofiolita cretácica se obtuvieron mediante sísmica de reflexión, registrada previamente en campañas de exploración petrolífera. La base del aluvial buza hacia el norte desde el pie de las Montañas del Norte de Omán, alcanzando una profundidad máxima de 2000m en la costa. Las distintas facies del aluvial están caracterizadas por rangos distintos, pero superpuestos, de resistividad eléctrica, dependiendo del contenido en arcillas y del grado de cementación. Las resistividades cerca de la costa se reducen por la intrusión salina. Estas variaciones de resistividad se obtuvieron con registros electromagnéticos a lo largo de un perfil de 400km, con el objetivo de distinguir las tres zonas del acuífero aluvial. También se pudo obtener la geometría de la cuña de intrusión, que llega hasta 10km de la costa. El espesor de las gravas saturadas que forman el acuífero oscila entre los 20-160m en un área superior a 600km2.

  18. The origin of increased salinity in the Cambrian-Vendian aquifer system on the Kopli Peninsula, northern Estonia

    NASA Astrophysics Data System (ADS)

    Karro, Enn; Marandi, Andres; Vaikmäe, Rein

    , qui renferme des eaux souterraines salines dans sa partie supérieure altérée et fissurée, et est hydrauliquement connecté avec l'aquifère supérieur du Cambrien-Vendien, est la deuxième importante source d'ions. Le soubassement fracturé et le matériel argileux de l'altération, renferme l'eau souterraine de type Ca-Cl, caractérisée par un haut TDS (2-20 g/l). A cause de la mobilisation intensive de l'eau les échanges d'eau souterraine est sont accélérés et la zone d'influence des pompages augmentent. Les études chimiques et isotopiques indiquent une contribution croissante du drainage des eaux du soubassement cristallin. L'intrusion d'eaux salées de la mer dans le système aquifère n'est pas un phénomène évident. El origen del incremento en salinidad en un sistema de acuíferos Cámbrico-Vendiano en la Península Kopli, norte de Estonie Monitoreo a largo plazo de un sistema de acuíferos confinados, de edad Cámbrico-Vendiano, que se utiliza como fuente de abastecimiento industrial en la Península Kopli, al norte de Estonie, revela cambios notables en la composición química del agua subterránea. Un incremento de 1.5 a 3 veces en TDS y en concentraciones de iones mayores en agua subterránea explotada ha sido ocasionado por bombeo fuerte. Las fuentes principales de carga disuelta en el agua subterránea Cámbrico-Vendiano son la lixiviación de la roca encajonante y los procesos geoquímicos que ocurren en la zona saturada. Basamento cristalino subyacente, que aloja agua subterránea salada en la parte superior intemperizada y fisurada, y está conectado hidráulicamente con el sistema acuífero Cámbrico-Vendiano sobreyacente, es la segunda fuente importante de iones. El basamento fracturado y su corteza de intemperismo arcillosa alojan agua subterránea de tipo Ca-Cl la cual se caracteriza por valores altos de TDS (2-20 g/l). Debido a extracción intensiva se ha acelerado el intercambio de agua subterránea y se ha incrementado el área de

  19. Perceptional gaps among women, husbands and family members about intentions for birthplace: a cross-sectional study.

    PubMed

    Shimpuku, Yoko; Madeni, Frida Elikana; Horiuchi, Shigeko; Leshabari, Sebalda Charles

    2017-01-30

    (BIQ), medindo o conhecimento, atitude, percepcão de controle comportamental, normas subjetivas e intenção para lugar de parto. Análise descritivas, ANOVA, Qui-quadrado e regressão linear múltipla foram utilizados para analisar os dados. o modelo de regressão mostrou que o conhecimento, percepcão de controle comportamental e normas subjetivas previram intenção de lugar de parto (R2 = 0,28). Enquanto 81% das mulheres grávidas pensavam que seus maridos eram os decisores para seu parto, apenas 38% dos maridos e 37% dos membros da família concordaram. As mulheres grávidas tiveram escores significativamente mais baixos sobre o item "Vou me preparar para o parto com a minha família", em comparação com os maridos (p < 0,01) e outros membros da família (p < 0,001). Proporcionar preparação para o parto baseada em evidencias e a redução das lacunas de percepção identificadas podem melhorar a intenção das mulheres para dar à luz em unidades de saúde. las mujeres son más propensas a dar a luz en un centro de salud cuando sus familias están de acuerdo con el lugar de parto. Sin embargo, en las zonas rurales de Tanzania, las mujeres son a menudo marginadas de la toma de decisiones. Este estudio predijo la intención del lugar de parto y los factores identificados para reducir la brecha de percepción entre las mujeres embarazadas, esposos y miembros de la familia. encuesta transversal explicativa llevada a cabo en tres aldeas en el noreste de Tanzania. Los participantes fueron 138 mujeres embarazadas y sus familias, que respondieron al Cuestionario sobre Intención del Parto (BIQ), que mide el conocimiento, la actitud, percepción de control del comportamiento, las normas subjetivas y la intención de lugar de parto. Se utilizaron análisis descriptivo, ANOVA, Chi-cuadrado y regresión lineal múltiple para analizar los datos. el modelo de regresión mostró que el conocimiento, la percepción de control del comportamiento, y las normas subjetivas

  20. Book of Abstracts (Plasma-Surface Interaction and Processing of Materials)

    DTIC Science & Technology

    1989-09-22

    Capuj* and M. N. Jakas** Centro Atomico Bariloche , C.N.E.A. 8400 Bariloche, Argentina Analytical calculations have been done in order to evaluate...CHARACTERIZATION BY ION BEAM TECHNIQUE N. E. Capuj*, N. R. Arista, G. H. Lantchsner, J. C. Eckardt, and M. M. Jakas** Centro Atomico Bariloche , C.N.E.A. 8400

  1. 78 FR 13645 - Notice of Public Meetings for the Draft Environmental Impact Statement and Draft Clean Air Act...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-28

    ... Conformity Determination in an open house format. DoN representatives will be available at informational... Determination are available for public review at the following libraries: 1. City of El Centro Public Library, 1140 North Imperial Avenue, El Centro, California 92243. 2. City of Imperial Public Library, 200 West...

  2. Defense Technical Information Center Cataloging Guidelines

    DTIC Science & Technology

    1994-02-01

    24 Field 10 - Personal Authors ............................................................ 27 Field 11 - Date of...Stanford, Calif. 24 . If the subordinate element of a heading begins with a personal name, that name is written without rearrangement. APPEARS AS...country is not repeated if ’ncluded in the heading. APPEARS AS: ENTER AS: Centro Brasileiro de Pesquisas Centro Brasileiro de Fisicas, Rio de Janeiro

  3. Differential Dengue Tropism & Neutralization: Potential Mechanisms of Pathogenesis

    DTIC Science & Technology

    2006-01-04

    Dr. Guillermo Comach and Dr. Daria Camacho, Laboratorio Regional de Diagnostico e Investigacion del Dengue y otras Enfermedades Virales, Centro de... Enfermedades Virales, Centro de Investigaciones Biomedicas de la Universidad de Carabobo (LARDIDEV/BIOMED- UC), Maracay, Venezuela. 4Naval Medical Research

  4. 47 CFR 73.622 - Digital television table of allotments.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 44c Coalinga *22 Concord 63c Corona 39 Cotati *23c El Centro 22, 48 Eureka *11, 16, 17, 28 Fort Bragg..., 43 Clovis 43 Concord 14 Corona 39 Cotati *23 El Centro 9, 22 Eureka 3, *11, 17, 28 Fort Bragg 8...

  5. 47 CFR 73.622 - Digital television table of allotments.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 44c Coalinga *22 Concord 63c Corona 39 Cotati *23c El Centro 22, 48 Eureka *11, 16, 17, 28 Fort Bragg... Clovis 43 Concord 14 Corona 39 Cotati *23 El Centro 9, 22 Eureka 3, *11, 17, 28 Fort Bragg 8 Fresno 7, 30...

  6. 47 CFR 73.622 - Digital television table of allotments.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 44c Coalinga *22 Concord 63c Corona 39 Cotati *23c El Centro 22, 48 Eureka *11, 16, 17, 28 Fort Bragg... Clovis 43 Concord 14 Corona 39 Cotati *23 El Centro 9, 22 Eureka 3, *11, 17, 28 Fort Bragg 8 Fresno 7, 30...

  7. 47 CFR 73.622 - Digital television table of allotments.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 44c Coalinga *22 Concord 63c Corona 39 Cotati *23c El Centro 22, 48 Eureka *11, 16, 17, 28 Fort Bragg..., 43 Clovis 43 Concord 14 Corona 39 Cotati *23 El Centro 9, 22 Eureka 3, *11, 17, 28 Fort Bragg 8...

  8. Proceedings of the 30th Annual Precise Time and Time Interval (PTTI) Systems and Applications Meeting

    DTIC Science & Technology

    1999-01-01

    roa.es C. Tomis RedIRIS, Centro Comunicaciones CSIC RedIRIS Serrano 142, 28006 Madrid, Spain tel + 34-91-585-5150 fax +34-91-564-7421 e-mail...I&D) and managed by the Centro de Comunicaciones CSIC RedIRIS, which depends on the Scientific Research Council (Consejo Superior de Investigaciones

  9. South Atlantic Conflict of 1982: A Case Study in Military Cohesion

    DTIC Science & Technology

    1988-04-01

    Level Technical School); Centro de Instruction de Inteligencia (Center for Intelligence Instruction) and Centro de Altos Estudios (The Center for...pero plenos de emocion y grandeza. (1987). La Semana, 12-15. Gal, R. (1985). Committment and obedience in the military: An Israeli case study. Armed

  10. Single stars in the Hyades open cluster. Fiducial sequence for testing stellar and atmospheric models

    NASA Astrophysics Data System (ADS)

    Kopytova, Taisiya G.; Brandner, Wolfgang; Tognelli, Emanuele; Prada Moroni, Pier Giorgio; Da Rio, Nicola; Röser, Siegfried; Schilbach, Elena

    2016-01-01

    Context. Age and mass determinations for isolated stellar objects remain model-dependent. While stellar interior and atmospheric theoretical models are rapidly evolving, we need a powerful tool to test them. Open clusters are good candidates for this role. Aims: We aim to create a fiducial sequence of stellar objects for testing stellar and atmospheric models. Methods: We complement previous studies on the Hyades multiplicity by Lucky Imaging observations with the AstraLux Norte camera. This allows us to exclude possible binary and multiple systems with companions outside a 2-7 AU separation and to create a single-star sequence for the Hyades. The sequence encompasses 250 main-sequence stars ranging from A5V to M6V. Using the Tool for Astrophysical Data Analysis (TA-DA), we create various theoretical isochrones applying different combinations of interior and atmospheric models. We compare the isochrones with the observed Hyades single-star sequence on J vs. J-Ks, J vs. J-H, and Ks vs. H-Ks color-magnitude diagrams. As a reference we also compute absolute fluxes and magnitudes for all stars from X-ray to mid-infrared based on photometric measurements available in the literature(ROSAT X-ray, GALEX UV, APASS gri, 2MASS JHKs, and WISE W1 to W4). Results: We find that combinations of both PISA and DARTMOUTH stellar interior models with BT-Settl 2010 atmospheric models describe the observed sequence well. We use PISA in combination with BT-Settl 2010 models to derive theoretical predictions for physical parameters (Teff, mass, log g) of 250 single stars in the Hyades. The full sequence covers the mass range of 0.13-2.30 M⊙, and effective temperatures between 3060 K and 8200 K. Conclusions: Within the measurement uncertainties, the current generation of models agree well with the single-star sequence. The primary limitations are the uncertainties in the measurement of the distances to individual Hyades members, and uncertainties in the photometry. Gaia parallaxes

  11. Aging and health: Self-efficacy for Self-direction in Health Scale.

    PubMed

    Oliveira, Albertina L; Silva, José T; Lima, Margarida P

    2016-07-04

    To validate the Escala de Autoeficácia para a Autodireção na Saúde (EAAS - Self-efficacy for Self-direction in Health Scale). Non-experimental quantitative study of EAAS validation, by confirmatory factorial analyses, evaluating a sample of 508 older adults from the north and the center of Portugal with mean age of 71.67 (from 51 to 96 years), to whom the Self-efficacy for Self-direction in Health Scale, the Rosenberg Self-esteem Scale, the Positive and Negative Affect Schedule, the Satisfaction with Life Scale, and the Instrumental Activities of Daily Living Scale were applied. The EAAS was developed from the theoretical constructs of self-efficacy and from self-directed learning within the PALADIN European project framework, aiming to develop an instrument able to assess the extent to which older adults take good care of their health. The internal consistency was 0.87 (Cronbach's alpha) and confirmatory factorial analyses enabled to find a model near the one theoretically proposed, indicating a structure consisting of four dimensions: physical exercise, healthy diet, engaging in health-related learning, and visits to health professionals. From the psychometric point of view, the model in four factors showed quite satisfactory fit indicators. The Self-efficacy for Self-direction in Health Scale, with 16 items, is adequate to evaluate to what extent older adults have confidence in their ability to take care of their own health, with high degree of autonomy. Validar a Escala de Autoeficácia para a Autodireção no domínio da Saúde (EAAS). Estudo quantitativo não experimental de validação da EAAS, por meio de análises fatoriais confirmatórias, avaliando amostra de 508 seniores e idosos provenientes das regiões Norte e Centro de Portugal com média etária de 71.67 (51 a 96 anos), a quem foram aplicadas a Escala de Autoeficácia para a Autodireção na Saúde, a Escala de Autoestima de Rosenberg, a Escala de Afeto Positivo e Afeto Negativo, a Escala de

  12. Groundwater-flow modeling in the Yucatan karstic aquifer, Mexico

    NASA Astrophysics Data System (ADS)

    González-Herrera, Roger; Sánchez-y-Pinto, Ismael; Gamboa-Vargas, José

    2002-09-01

    aquifère. La zone karstifiée a été modélisée en posant l'hypothèse qu'il fonctionne hydrauliquement comme un milieu poreux granulaire. Au cours de la calibration, les hypothèses suivantes ont été testées: (1) les phénomènes karstiques jouent un rôle important dans le système aquifère, (2) un anneau ou une ceinture de dépressions dans la région est la manifestation d'une zone à forte transmissivité qui permet l'écoulement en conduits de l'eau souterraine en direction du Golfe du Mexique, et (3) la situation géologique dans la partie sud du Yucatan détermine les écoulements souterrains. Le modèle montre que la faille de la Sierrita de Ticul, dans la partie sud-ouest de la région étudiée, joue le rôle de barrière et que les valeurs de la piézométrie décroissent en direction du nord-est. La modélisation montre également que la dynamique du système aquifère à l'échelle régionale n'a pas été modifiée malgré le grand nombre de puits de pompage, parce que le volume pompé est faible en comparaison du volume de recharge; en outre, le réseau karstique très bien développé dans cette région possède une très forte conductivité hydraulique. Resumen. El modelo conceptual actual del acuífero cárstico no confinado de la Península de Yucatán (México) es el de un lentejón de agua dulce flotando sobre agua salada, más densa, la cual penetra más de 40 kilómetros tierra adentro. Debido a la alta conductividad hidráulica del acuífero, existe un gradiente hidráulico muy bajo cuyo rango está entre 7 y 10 milímetros por kilómetro en la porción norte de la península. Se utilizó el código AQUIFER para investigar el sistema de flujo de las aguas subterráneas a escala regional en el acuífero. La zona carstificada se modeló suponiendo que actúa hidráulicamente como un medio poroso granular. Como parte de la calibración, se probaron las siguientes hipótesis: (1) las características cársticas desempeñan un papel importante

  13. Sociodemographic profile of medicines users in Brazil: results from the 2014 PNAUM survey.

    PubMed

    Bertoldi, Andréa Dâmaso; Pizzol, Tatiane da Silva Dal; Ramos, Luiz Roberto; Mengue, Sotero Serrate; Luiza, Vera Lucia; Tavares, Noemia Urruth Leão; Farias, Mareni Rocha; Oliveira, Maria Auxiliadora; Arrais, Paulo Sergio Dourado

    2016-12-01

    , Utilização e Promoção do Uso Racional de Medicamentos (PNAUM), pesquisa nacional de base populacional, realizada entre setembro de 2013 e fevereiro de 2014 em residências de municípios urbanos. Avaliou-se o uso de todos os medicamentos (global), uso de medicamentos para doenças crônicas e uso de medicamentos para doenças agudas. As variáveis independentes utilizadas foram sexo, idade, classificação econômica e região do País. Foram calculados prevalências e intervalos de confiança de 95% (IC95%) e aplicado teste Qui-quadrado de Pearson para avaliação das diferenças entre os grupos, considerando o nível de significância de 5%. A prevalência global de uso de medicamentos foi de 50,7% (IC95% 49,3-52,2), sendo 39,3% (IC95% 37,5-41,1) no sexo masculino e 61,0% (IC95% 59,3-62,6) no sexo feminino. Observou-se aumento nas prevalências de uso com a idade (exceto de zero a quatro anos). As menores prevalências de uso ocorreram no grupo mais pobre e na região Norte do País. A prevalência de uso de medicamentos para doenças crônicas foi de 24,3% (IC95% 23,3-25,4) e para doenças agudas foi de 33,7% (IC95% 32,1-35,4). Existe grande variabilidade nas prevalências globais de uso de medicamentos por regiões brasileiras. As regiões consideradas mais pobres (Norte, Nordeste e Centro-Oeste) apresentam menores prevalências de uso de medicamentos para doenças crônicas, o que indica a necessidade de minimizar as desigualdades no acesso aos medicamentos dentro do País.

  14. Assessment of unconventional tight-gas resources of the Magallanes Basin Province, Chile, 2015

    USGS Publications Warehouse

    Schenk, Christopher J.; Charpentier, Ronald R.; Pitman, Janet K.; Tennyson, Marilyn E.; Brownfield, Michael E.; Gaswirth, Stephanie B.; Le, Phuong A.; Leathers-Miller, Heidi M.; Marra, Kristen R.

    2016-01-20

    Using a geology-based assessment methodology, the U.S. Geological Survey assessed a technically recoverable mean resource of 8.3 trillion cubic feet of unconventional tight gas in the Zona Glauconitica of the Magallanes Basin Province, Chile.

  15. 77 FR 33766 - Notice of Public Meeting; Wyoming Resource Advisory Council

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-07

    ... the BLM's wild horse partnership with Friends of a Legacy and Marathon Oil, water projects, porcine zona pellucida and the overall wild horse program; a RAC business session; and a presentation on fire...

  16. A Modular, Reconfigurable Surveillance UAV Architecture

    DTIC Science & Technology

    2003-09-02

    Una Società Galileo Avionica A Modular, Reconfigurable Surveillance UAV Architecture METEOR, Finmeccanica Group Zona Industriale di Soleschiano Via...ES) METEOR, Finmeccanica Group Zona Industriale di Soleschiano Via Mario Stoppani 21 34077 Ronchi dei Legionari (GO) ITALY 8. PERFORMING...PMSFMS RS1Backup FMS NSU Payload Control Actuators Router Router RS2 Recovery Devices Una Società Galileo Avionica • Daylight TV Camera • IR Sensor • HR

  17. Effects of acute administration of ethanol on the rat adrenal cortex.

    PubMed

    Milovanović, Tatjana; Budec, Mirela; Balint-Perić, Ljiljana; Koko, Vesna; Todorović, Vera

    2003-09-01

    The purpose of this study was to investigate the effect of a single dose of ethanol on rat adrenal cortex and to determine whether the estrous cycle can influence this effect of ethanol. Adult female Wistar rats showing proestrus or diestrus Day 1 (n = 12) were treated intraperitoneally with ethanol (4 g/kg body weight). Untreated (n = 15) and saline-injected (n = 14) rats were used as controls. The animals were sacrificed by decapitation 0.5 hour after ethanol administration. Stereological analysis was performed on paraffin sections of adrenal glands stained with AZAN, and the following parameters were determined: absolute volume of the zona glomerulosa, the zona fasciculata and the zona reticularis, numerical density, volume and the mean diameter of adrenocortical cells and of their nuclei, and diameter and length of capillaries. The diameter and volume of adrenocortical cells in the zona fasciculata and the zona reticularis were significantly increased by acute ethanol treatment at proestrus. In the same group of animals, a single dose of ethanol induced significant decrease in numerical density of adrenocortical cells and of their nuclei in all three zones. Increased length of capillaries of the zona fasciculata as well as enhanced level of serum corticosterone was found in ethanol-treated rats at both phases of the estrous cycle, proestrus and diestrus Day 1. The obtained results indicate that a single dose of ethanol activates adrenal cortex in female rats and that the effect is more pronounced on morphometric parameters at proestrus.

  18. The ants of North and Central America: the genus Mycocepurus (Hymenoptera: Formicidae)

    PubMed Central

    Mackay, William P.; Maes, Jean-Michel; Fernández, Patricia Rojas; Luna, Gladys

    2004-01-01

    Abstract We provide a review of the North American ants (north of Colombia) of the ant genus Mycocepurus, including keys to the workers and females, illustrations and distribution maps. The distribution of M. tardus is extended to Nicaragua and Costa Rica. The female of M. curvispinosus is described. Resumen Se revisan las especies del género Mycocepurus de Norte América (al norte de Colombia). Se incluyen claves para la identificación de las obreras y las hembras, ilustraciones y mapas de distribución. Se amplia hacia el norte la distribución de M. tardus, incluyendo ahora Nicaragua y Costa Rica y se describe la hembra de M. curvispinosus. PMID:15861242

  19. Species profiles: Life histories and environmental requirements of coastal fishes and invertebrates (Pacific Northwest)--ghost and blue mud shrimp

    USGS Publications Warehouse

    Hornig, S.; Sterling, A.; Smith, Styles

    1989-01-01

    Geographic range: The ghost shrimp is found in intertidal areas along the west coast of North America from Mutiny Bay, Alaska, to the mouth of the Tijuana River, San Diego County, California; MacGinitie (1934) and Ricketts and Calvin (1968) reported finding specimens as far south as El Estuario de Punto Banda, Baja California Norte, Mexico. The blue mud shrimp is found from southeastern Alaska to San Quentin Bay (Bahia de San Quentin) in Baja California Norte. The general distribution of the two species in the Pacific Northwest is identical (Figure 3).

  20. Downstream of downtown: urban wastewater as groundwater recharge

    NASA Astrophysics Data System (ADS)

    Foster, S. S. D.; Chilton, P. J.

    Wastewater infiltration is often a major component of overall recharge to aquifers around urban areas, especially in more arid climates. Despite this, such recharge still represents only an incidental (or even accidental) byproduct of various current practices of sewage effluent handling and wastewater reuse. This topic is reviewed through reference to certain areas of detailed field research, with pragmatic approaches being identified to reduce the groundwater pollution hazard of these practices whilst attempting to retain their groundwater resource benefit. Since urban sewage effluent is probably the only `natural resource' whose global availability is steadily increasing, the socioeconomic importance of this topic for rapidly developing urban centres in the more arid parts of Asia, Africa, Latin America and the Middle East will be apparent. L'infiltration des eaux usées est souvent la composante essentielle de toute la recharge des aquifères des zones urbaines, particulièrement sous les climats les plus arides. Malgré cela, une telle recharge ne constitue encore qu'un sous-produit incident, ou même accidentel, de pratiques courantes variées du traitement de rejets d'égouts et de réutilisation d'eaux usées. Ce sujet est passé en revue en se référant à certaines régions étudiées en détail, par des approches pragmatiques reconnues pour permettre de réduire les risques de pollution des nappes dues à ces pratiques tout en permettant d'en tirer profit pour leur ressource en eau souterraine. Puisque les effluents d'égouts urbains sont probablement la seule « ressource naturelle » dont la disponibilité globale va croissant constamment, l'importance socio-économique de ce sujet est évidente pour les centres urbains à développement rapide de l'Asie, de l'Afrique, de l'Amérique latine et du Moyen-Orient. La infiltración de aguas residuales es a menudo un componente principal de la recarga total en acuíferos ubicados en torno a zonas urbanas

  1. Petrography and Geochemistry of the Zamora Batholith in the south of the sub-Andean zone (Ecuador)

    NASA Astrophysics Data System (ADS)

    Villares, F. M.

    2013-05-01

    The Zamora Batholith is an intrusive complex that is located in the extreme south-east of Ecuador. It has dimensions of 200 x 50 km approximately. It is mainly located in the Zamora Chinchipe province from which it takes its name. This study consisted in the petrographic and geochemical characterization of the Zamora Batholith in the area covered by 1: 50,000 geological maps of Centro Shaime, Guayzimi, Paquisha, Los Encuentros and El Pangui. Fieldwork was done by the "Proyecto Mapeo Geológico escala 1:50.000 (zonas prospectivas mineras)" of the Instituto Nacional de Investigación Geológico, Minero, Metalúrgico of Ecuador. This research was performed with 59 thin sections and 10 whole - rock chemical analysis done in the C.I.C of the Granada University. The Zamora Batholith intrudes Triassic to Jurassic volcanic rocks. It is overlaid by sandstones of the Hollin Formation of the Upper Aptian age and shale and limestone from the Napo Formation. Post-cretaceous deposits of ash and lava flows of andesitic to rhyolitic compositions cover the batholith. The petrography of the Zamora Batholith ranges from tonalite to monzogranite with the same qualitative mineralogy. The rocks are composed by different proportions of plagioclase, amphibole, feldspar K, quartz, biotite, opaque, pyroxene and epidote, as accessory minerals has zircon, sphene and apatite. To the south of the Conguime and Guayzimi towns, the dominant petrography is medium to coarse grained amphibole granodiorite with tonalitic and monzogranitic subordinates. To the north monzogranites are dominant rocks and subordinate granodiorites. To the East of Santa Elena the sienogranites are associated with El Hito porphyritic granite that intrudes to Zamora Batholith. Frequently the batholith has propylitic alteration; which produces a primary association of chlorite, epidote, calcite and pyrite. The granitoids have dioritic to granitic compositions (60.09 to 73.6 wt.% SiO2) and are I - type, medium to high-K calc

  2. Recombinant mouse sperm ZP3-binding protein (ZP3R/sp56) forms a high order oligomer that binds eggs and inhibits mouse fertilization in vitro.

    PubMed

    Buffone, Mariano G; Zhuang, Tiangang; Ord, Teri S; Hui, Ling; Moss, Stuart B; Gerton, George L

    2008-05-02

    Many candidates have been proposed as zona pellucida-binding proteins. Without precluding a role for any of those candidates, we focused on mouse sperm protein ZP3R/sp56, which is localized in the acrosomal matrix. The objective of this study was to analyze the role of ZP3R/sp56 in mouse fertilization. We expressed recombinant ZP3R/sp56 as a secreted protein in HEK293 cells and purified it from serum-free, conditioned medium. In the presence of reducing agents, the recombinant ZP3R/sp56 exhibited a molecular weight similar to that observed for the native ZP3R/sp56. Reminiscent of the native protein, recombinant ZP3R/sp56 formed a high molecular weight, disulfide cross-linked oligomer consisting of six or more monomers under non-reducing conditions. Recombinant ZP3R/sp56 bound to the zona pellucida of unfertilized eggs but not to 2-cell embryos, indicating that the changes that take place in the zona pellucida at fertilization affected the interaction of this protein with the zona pellucida. The extent of in vitro fertilization was reduced in a dose-dependent manner when unfertilized eggs were preincubated with recombinant ZP3R/sp56 (74% drop at the maximum concentrations assayed). Eggs incubated with the recombinant protein showed an absence of or very few sperm in the perivitelline space, suggesting that the reduction in the fertilization rate is caused by the inhibition of sperm binding and/or penetration through the zona pellucida. These results indicate that sperm ZP3R/sp56 is important for sperm-zona interactions during fertilization and support the concept that the acrosomal matrix plays an essential role in mediating the binding of sperm to the zona pellucida.

  3. 77 FR 20690 - Environmental Impact Statement: Albuquerque, New Mexico

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-05

    ...: Albuquerque, New Mexico AGENCY: Federal Highway Administration (FHWA), USDOT. ACTION: Rescind Notice of Intent... proposed improvements to the Interstate 25 and Paseo del Norte Interchange in Albuquerque, New Mexico. FOR... Mexico Division, 4001 Office Court Drive, Suite 801, Santa Fe, New Mexico 87507, Telephone (505) 820-2027...

  4. Hydrologic and Water Quality Assessment from Managed Turf

    USDA-ARS?s Scientific Manuscript database

    The potential for nutrients and pesticides to be transported to surface water from turf systems (especially golf courses) is often debated because of limited information on water quality exiting these systems. This four year study quantified the amount and quality of water draining from part of Nort...

  5. Keeping Score without the Body Count: Measuring Counterinsurgent Effectiveness According to Insurgent Tangible Resources

    DTIC Science & Technology

    2013-05-23

    down tanks. The tank’s advantage is so obvious that the image is almost comical . An insurgent also typically cannot penetrate the base perimeter of a......92. 32 Ibid., 93. 33 Mao Zedong, The Red Book of Guerrilla Warfare, trans. Chen Song, ed. Shawn Conners (El Paso, TX: Norte Press, 2008) Kindle

  6. 45. C.P.S., photographer January 1936 MENDOCINO COUNTY, SECTION, HIGHWAY 1. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    45. C.P.S., photographer January 1936 MENDOCINO COUNTY, SECTION, HIGHWAY 1. MEN-I-J #132, 388+00 81TTC2, LOOKING SOUTH LANES FLAT, C.P.S., 1-36. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  7. Programa de conservacion para aves migratorias neotropicales

    Treesearch

    Deborah Finch; Marcia Wilson; Roberto Roca

    1992-01-01

    Mas de 250 especies de aves terrestres migran a Norte America durante la epoca reproductiva para aprovechar los sistemas templados. No obstante, las aves migratorias neotropicales pasan la mayor parte de su ciclo de vida en los habitat tropicales y subtropicales de paises latinoamericanos y caribefios donde viven en una asociacion cercana con las aves residentes. Para...

  8. Adolescent Drug Use in Mexico and among Mexican American Adolescents in the United States: Environmental Influences and Individual Characteristics.

    ERIC Educational Resources Information Center

    Felix-Ortiz, Maria; Velazuez, Jorge A Villatoro; Medina-Mora, Maria Elena; Newcomb, Michael D.

    2001-01-01

    Compares cigarette, alcohol, and illegal drug use among high school students in Baja California Norte (BCN), Mexico with Mexican American students in Los Angeles (LA), California (N=516). Demographic variables, individual characteristics, and environmental influences were considered. Reports that more BCN students used alcohol and more LA students…

  9. Two new species of Aulospongus Norman, 1878 with a key to the Atlantic species (Poecilosclerida; Demospongiae; Porifera).

    PubMed

    Cavalcanti, Thaynã; Santos, George Garcia; Pinheiro, Ulisses

    2014-07-03

    We describe two new species: Aulospongus trirhabdostylus sp. nov. and Aulospongus mandela sp. nov. from Potiguar Basin (Rio Grande do Norte State, Northeastern Brazil). Both species were compared with their congeners and an identification key for the Atlantic species of Aulospongus is provided. The genus Aulospongus now contains 16 species.

  10. Astronomy in Syria

    NASA Astrophysics Data System (ADS)

    Al-Mousli, A. T.

    2006-11-01

    Syria has been involved in the field of astronomy since 1997, when Prof. F.R. QUERCI, France, visited Syria and made a presentation on the International NORT project; (NORT: the Network of Oriental Robotic Telescope), which was a selected project of the sixth United Nations/ European Space Agency Workshop on Basic Space Science (document no. A/AC.105/657 dated 13/12/1996). NORT aims to establish a robotic telescope network on high mountain peaks around the Tropic of Cancer, from Morocco in the west to the desert of China in the east. The purposes for establishing this network are technical and educational. The General Organization of Remote Sensing (GORS) has carried out a pilot study using remote sensing techniques and has selected four sites in order to determine the best location for the astronomical observatory the within NORT programme. Following this project, GORS decided to establish an office for astronomical studies, one of the earliest works of GORS in astronomy was an initiative to establish a planetarium within the GORS campus, to accommodate approximately 120 observers. A contest to choose the best planetarium design, for the Arab World, took place at GORS.

  11. Timber resource statistics for the North Coast resource area of California 1994.

    Treesearch

    Karen L. Waddell; Patricia M. Bassett

    1996-01-01

    This report is a summary of timber resource statistics for the North Coast Resource Area of California, which includes Del Norte, Humboldt, Mendocino, and Sonoma Counties. Data were collected by the Pacific Northwest Research Station as part of a State-wide multi-resource inventory. The inventory sampled private and public lands except reserved areas and National...

  12. Final Supplemental Environmental Assessment: Falcon I Launch Vehicle Program from SLC-4W Vandenberg Air Force Base, California

    DTIC Science & Technology

    2005-09-06

    affected surface water, 3) adversely affected groundwater quantity or quality, or 4) caused a need that exceeded the existing potable supply or...goby is from Tillas Slough (mouth of the Smith River) in Del Norte County, south to Colonel Louis D. Van Mullem, Jr. (1-8-96-F/C-29) 5 Agua Hedionda

  13. 77 FR 77007 - Transfer of Land to the Department of Interior

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ... Secretary of the Interior, to be held in trust for the Yurok Tribe. The lands are described as follows: Six...., approved June 24, 1971, and (1) excepting from lands in Sec. 10, a piece or parcel of land conveyed to the..., on pages 92 to 95 of Book 54 of Deeds, Records of Del Norte County, California, said piece or parcel...

  14. The timber resources of Humboldt County, California.

    Treesearch

    Daniel D. Oswald

    1968-01-01

    This report presents the first complete inventory of Humboldt County's timber resources. Past Forest Survey inventories have included Humboldt County, but they were not designed to obtain volume estimates for an individual county. Humboldt County is part of a survey unit which also includes Del Norte County. There are eight such inventory units in California; and...

  15. Descripción y distribución de Hydnotrya cerebriformis (Discinaceae: Pezizales) en México. Description of Hydnotrya cerebriformis (Discinaceae: Pezizales) from Mexico

    Treesearch

    Carolina Piña-Páez; Roberto Garibay-Orijel; Gonzalo Guevara-Guerrero; Michael A. Castellano

    2017-01-01

    El género Hydnotrya comprende alrededor de 13 especies de Ascomicetos hipogeos, secuestrados ectomicorrízicos distribuidos exclusivamenteen bosques templados del hemisferio norte. En este trabajo se describe e ilustra por primera vez una especie de este género, Hydnotrya cerebriformis,para México. Esta especie se distribuye en...

  16. 38. LANDSCAPE HIGHWAY VIEW, OREGON STATE HIGHWAY 199. JOSPHINE COUNTY, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    38. LANDSCAPE HIGHWAY VIEW, OREGON STATE HIGHWAY 199. JOSPHINE COUNTY, OREGON. REEVES CREEK (WHERE ROAD WIDENS) 4 MILES NE OF CAVE JUNCTION. LOOKING S. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  17. Do the Factors Affecting Academic Achievement Differ by the Socio-Economic Status or Sex of the Student? A Puerto Rican Secondary School Sample. Final Report.

    ERIC Educational Resources Information Center

    Nuttall, Ronald L.

    Variables expected to be associated with academic achievement were examined in a sample (generally exceeding 2500) from eight secondary schools in Baymon Norte, Puerto Rico. Concern was whether variables associated with academic achievement differed by sex or by socioeconomic status (SES). Multivariate analyses of variance with three factors of…

  18. Prioritizing treatment of second-growth forests using LiDAR

    Treesearch

    Lathrop P. Leonard; Daryl Van Dyke

    2012-01-01

    We used multi-return light detecting and ranging (LiDAR) to develop a costeffective method for describing forest conditions and prioritizing stands for treatment in over 14,000 ha of second-growth forests (11 to 85 years old) in Del Norte Coast Redwoods State Park (DNCRSP). DNCRSP consists primarily of redwood and Douglas-fir dominated forests with scattered tanoak...

  19. Philippine Classroom Teachers as Researchers: Teachers' Perceptions, Motivations, and Challenges

    ERIC Educational Resources Information Center

    Ulla, Mark B.; Barrera, Kenneth Ian B.; Acompanado, Meller M.

    2017-01-01

    This study explores teachers' perceptions and motivations, challenges, and needs of 50 teachers in Agusan del Norte, Philippines with regards to doing research. Methodologies used were survey questionnaire, and group and individual interviews. Findings revealed that teacher-respondents had a positive perceptions towards doing research and its…

  20. 46. C.P.S., photographer March 1936 MENDOCINO COUNTY, SECTION, HIGHWAY 1. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    46. C.P.S., photographer March 1936 MENDOCINO COUNTY, SECTION, HIGHWAY 1. MEN-1-J#135, 384+50 81TTC2, LOOKING SOUTH LANES FLAT, C.P.S., 36. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  1. "Mexico in Transition." Curriculum Units.

    ERIC Educational Resources Information Center

    Oregon Univ., Eugene. Foreign Language Resource Center.

    These curriculum units were developed in a National Endowment for the Humanities 1994 summer seminar "Mexico in Transition." The 23 lessons are written in Spanish. Lessons are entitled: (1) "La Migracion Mexicana Vista a Traves del Cuento 'Paso del Norte' de Juan Rulfo" (Jose Jorge Armendariz); (2) "Los Grupos Indigenas de…

  2. DRINKING WATER ISSUES

    EPA Science Inventory

    According to recent reports by the California Department of Health Services, the State of Maine, and the United State Geological Survey (USGS); the fuel oxygenate methyl teri-butyl ether (MTBE) is present in 5 to 20 percent of the drinking water sources in California and the nort...

  3. 1. CONTEXTUAL VIEW OF BRIDGE IN SETTING, LOOKING SOUTHWEST, FROM ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. CONTEXTUAL VIEW OF BRIDGE IN SETTING, LOOKING SOUTHWEST, FROM DOWNSTREAM. Crew, vehicles, boats, and equipment are from the California Department of Transportation's Transportation Laboratory conducting test borings for the replacement bridge. - Smith River Bridge, CA State Highway 199 Spanning Smith River, Crescent City, Del Norte County, CA

  4. Schizopolis: Border Cinema and the Global City (of Angels)

    ERIC Educational Resources Information Center

    Fojas, Camilla

    2006-01-01

    The films "Star Maps" and "El Norte" reveal the real political, socioeconomic, and psychic costs of divided cities by bringing the simulated realities of mass media to their logical end, thereby sacrificing each character to the disorder of the city. In "Star Maps", Carlos dreams of becoming the next major Latino…

  5. Biology and population dynamics of the cactus moth, Cactoblastis cactorum

    USDA-ARS?s Scientific Manuscript database

    The cactus moth, Cactoblastis cactorum, was a successful biological control agent against prickly pear cacti in Australia in the 1920’s. Since then, it was introduced to other countries including the Carribean islands. In 1989, the cactus moth was reported in Florida and has continued to spread nort...

  6. RAPID ASSESSMENT OF URBAN WETLANDS: FUNCTIONAL ASSESSMENT MODEL DEVELOPMENT AND EVALUATION

    EPA Science Inventory

    The objective of this study was to test the ability of existing hydrogeomorphic (HGM) functional assessment models and our own proposed models to predict rates of nitrate production and removal, functions critical to water quality protection, in forested riparian wetlands in nort...

  7. 57. G.M.W., photographer March 26, 1940 HUMBOLDT COUNTY, SECTION K, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    57. G.M.W., photographer March 26, 1940 HUMBOLDT COUNTY, SECTION K, HIGHWAY 1. HUM-1-K #93, STA. 255, 3-26-40, G.M.W. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  8. 47. N.R.P., photographer January 14, 1938 MENDOCINO COUNTY, SECTION, HIGHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    47. N.R.P., photographer January 14, 1938 MENDOCINO COUNTY, SECTION, HIGHWAY 1. 1-MEN-1-J #144, ROCK CREEK BRIDGE. SO., 1-14-38, N.R.P. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  9. PCR reveals high prevalence of non-sporulating Nosema bombi(Microsporidia) infections in bumble bees (Bombus)in northern Arizona

    USDA-ARS?s Scientific Manuscript database

    About 20% of bumble bee species are in decline in North America, and the microsporidian pathogen, Nosema bombi, may be a factor in these declines. We performed a comprehensive survey of N. bombi infections in the bumble bee communities throughout the flight season along an elevation gradient in Nort...

  10. Individual-based model formulation for cutthroat trout, Little Jones Creek, California

    Treesearch

    Steven F. Railsback; Bret C. Harvey

    2001-01-01

    This report contains the detailed formulation of an individual-based model (IBM) of cutthroat trout developed for three study sites on Little Jones Creek, Del Norte County, in northwestern California. The model was designed to support research on relations between habitat and fish population dynamics, the importance of small tributaries to trout populations, and the...

  11. Maritime Factors Affecting Iberian Security,

    DTIC Science & Technology

    1980-10-01

    afirme/ que para las sovie’ticas una campaiia en gran escala dirigida contra las comunicaciones marftimas del Atlantica Norte durante el perfada inicial...defender sus l ’neas de comunicacion maritima; y cuarta -- y a mi parecer la consideracion mas importante -- las otras tareas que la marina de guerra

  12. Managing the Environmental Impacts of Growth Under Climate Change: A Workshop for State and Local Decision-Makers--Workshop Summary

    EPA Science Inventory

    From November 8/9, 2011, the U.S. Environmental Protection Agency (EPA) hosted a workshop titled "Managing the Environmental Impacts of Growth Under Climate Change." The Office of Research and Development (ORD) organized the meeting, which was held in Research Triangle Park, Nort...

  13. 59. V.H.G., photographer October 27, 1948 EUREKA, HUMBOLDT COUNTY, HIGHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    59. V.H.G., photographer October 27, 1948 EUREKA, HUMBOLDT COUNTY, HIGHWAY 1. HUM-1-EUR #59, LOOKING NORTH BROADWAY & CEDAR, 10-27-48, V.H.G. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  14. 58. V.H.G., photographer October 27, 1948 EUREKA, HUMBOLDT COUNTY, HIGHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    58. V.H.G., photographer October 27, 1948 EUREKA, HUMBOLDT COUNTY, HIGHWAY 1. HUM-1-EUR #60, LOOKING SOUTH, BROADWAY & CLARK, 10-27-48, V.H.G. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  15. 75 FR 71732 - Proposed Finding Against Federal Acknowledgment of the Tolowa Nation

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-24

    ... ancestors were involved in interaction indicative of a social community, but does not to show that they... social interaction. Researchers also consulted BIA enrollments conducted by Henry Roe Cloud in 1939. The... Rancherias in 1906, 1908, and 1938 respectively; and Federal interaction with the Del Norte Indian Welfare...

  16. Algunos Resumenes

    NASA Astrophysics Data System (ADS)

    1980-12-01

    EI "Palomar Observatory Sky Survey" es un medio auxiliar bien conocido y ütil para los astronomos. Todo el cielo dei hemisferio Norte esta captado en lotografias, cuyas reproducciones se encuentran archivadas en las bibliotecas de casi todos los observatorios importantes dei mundo.

  17. Environmentally friendly elimination of moss from open-graded asphalt pavement.

    DOT National Transportation Integrated Search

    2011-04-01

    Caltrans has discovered moss growing on U.S. Highway 101 in Del Norte and Humboldt counties, U.S. : Highway 199 near the Smith River and other roadways in Caltrans District 1. These areas are heavily : forested, and the heavy tree canopies shielding ...

  18. 77 FR 39413 - Safety Zone: Crescent City Fourth of July Fireworks Event, Crescent City, CA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-03

    ...-AA00 Safety Zone: Crescent City Fourth of July Fireworks Event, Crescent City, CA AGENCY: Coast Guard... the navigable waters near Crescent City Harbor in Crescent City, CA in support of the Crescent City... establish safety zones (33 U.S.C sections 1221 et seq.). Crescent City-Del Norte County Chamber of Commerce...

  19. 56. Photographer unknown February 1925 HUMBOLDT COUNTY, SECTION J, HIGHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    56. Photographer unknown February 1925 HUMBOLDT COUNTY, SECTION J, HIGHWAY 1. HUM-1-J #36, SINKS ALONG OCEAN SHORE, 2-25. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  20. 54. Photographer unknown February 1925 HUMBOLDT COUNTY, SECTION J, HIGHWAY ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    54. Photographer unknown February 1925 HUMBOLDT COUNTY, SECTION J, HIGHWAY 1. HUM-1-J #34, SINKS ALONG HIGH. NEAR OCEAN, 2-25. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  1. 11. VIEW, LOOKING SOUTHEAST, ALONG CENTERLINE FROM SOUTH CANTILEVER TOWARD ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. VIEW, LOOKING SOUTHEAST, ALONG CENTERLINE FROM SOUTH CANTILEVER TOWARD TOWARD NORTH PORTAL. Note vertical displacement in deck caused by partial collapse at point of connection between south cantilever arm and suspended span. - Smith River Bridge, CA State Highway 199 Spanning Smith River, Crescent City, Del Norte County, CA

  2. Examining the Impact of Academic Development in the Engineering Faculties in Chile: Changes in Teaching Philosophy and Teachers' Competencies

    ERIC Educational Resources Information Center

    Acosta Peña, Roxana; Tomás-Folch, Marina; Feixas, Mònica

    2017-01-01

    The Faculties of Engineering Sciences at Universidad Católica del Norte in Chile regard teacher training as a necessary tool for its academics' professional development and as a fundamental way to improve their teaching quality. The Teaching Unit for Innovation in Engineering (UIDIN) has developed a new curriculum and training programme which…

  3. Identification of novel QTL for sawfly resistance in wheat

    Treesearch

    J. D. Sherman; D. K. Weaver; M. L. Hofland; S. E. Sing; M. Buteler; S. P. Lanning; Y. Naruoka; F. Crutcher; N. K. Blake; J. M. Martin; P. F. Lamb; G. R. Carlson; L. E. Talbert

    2010-01-01

    The wheat stem sawfly (WSS) (Cephus cinctus Nort.) is an important pest of wheat (Triticum aestivum L. em. Thell.) in the Northern Great Plains. This paper reports the genetic analysis of antixenosis for egg-laying WSS females in recombinant inbred lines (RIL) of hard red spring wheat. Female WSS preferentially choose certain wheat genotypes for egg-laying, with the...

  4. Timber resource statistics of the north coast resource area of California.

    Treesearch

    J.D. Lloyd; Joel Moen; Charles L. Bolsinger

    1986-01-01

    This report is one of five that provide timber resource statistics for 57 of the 58 counties in California (San Francisco is excluded). This report presents statistics from a 1981-84 inventory of the timber resources of Del Norte, Humboldt, Mendocino, and Sonoma Counties. Tables presented are of forest area and of timber volume, growth, and mortality. The north coast...

  5. Hazardous Waste Cleanup: HQ 10th MTN Division & Fort Drum in Fort Drum, New York

    EPA Pesticide Factsheets

    HQ 10th MTN Division & Fort Drum facility is located at Jones Street and Off North Memorial Drive, in Fort Drum, in the northern portion of New York State approximately 10 miles northeast of Watertown. Fort Drum is the largest Army installation in the nort

  6. Breeding distribution of the marbled murrelet in Redwood National Park and vicinity during 1988

    Treesearch

    C. John Ralph; Peter W. C. Paton; Aivars Zakis; Gary Strachan

    1990-01-01

    We report on an intensive research effort to determine the present status of the marbled murrelet (Brachyramphus marmoratus) in the vicinity of Redwood National Park, in Del Norte and northern Humboldt Counties, California. This seabird is primarily an inhabitant of the nearshore waters and adjacent coastal redwood forests of the northern half of the...

  7. Monozygotic Twin Pregnancy Associated with In Vitro Fertilization: Report and Revision of Three Cases Presented in a Clinic at 10,925 Feet Above Sea Level.

    PubMed

    Tominaga, Luis Vargas; Cáceres, Ricardo Pella; Lechuga, Alberto Vargas; Durán, Livia Bartolo; Vargas, Mariela Serrano

    2015-08-01

    3 cases of monozygotic twin pregnancies associated with IVF presented at Centro de Fertilidad y Ginecología del Sur. In this study, we describe the cases and review the literature, noting possible related factors.

  8. Survival of sheep demi-embryos in vivo and in vitro.

    PubMed

    Shelton, J N; Szell, A

    1988-01-01

    Sheep embryos (morulae and blastocysts) were bisected either by microscalpel or by microneedle after dissolving the zona pellucida with acidified Tyrode's solution. Fourteen and 11 cryopreserved demi-embryos failed to develop when transferred to recipients or placed in culture, respectively. When fresh demi-embryos were cultured in Dulbecco's phosphate buffered saline (DPBS) plus fetal calf serum (FCS) or Whitten's medium, the survival rate was 26% compared to 68% for whole embryos (P<0.01), and there was a suggestion that the presence of a zona pellucida was beneficial to survival. When two demi-embryos each within a zona pellucida were transferred into each of 10 ewes, six of them lambed to produce a total of eight lambs, including two sets of identical twins. Of 10 ewes receiving two demi-embryos without zonae pellucidae, three lambed to produce a total of four lambs, including one set of identical twins. Of 10 ewes that each received two whole embryos, 10 lambed to produce a total of 16 lambs. There was a suggestion that the zona pellucida might enhance the survival of demi-morulae but not demi-blastocysts.

  9. Possible mechanism of polyspermy block in human oocytes observed by time-lapse cinematography.

    PubMed

    Mio, Yasuyuki; Iwata, Kyoko; Yumoto, Keitaro; Kai, Yoshiteru; Sargant, Haruka C; Mizoguchi, Chizuru; Ueda, Minako; Tsuchie, Yuka; Imajo, Akifumi; Iba, Yumiko; Nishikori, Kyoko

    2012-09-01

    To analyze the fertilization process related to polyspermy block in human oocytes using an in vitro culturing system for time-lapse cinematography. We had 122 oocytes donated for this study from couples that provided informed consent. We recorded human oocytes at 2,000 to 2,800 frames every 10 s during the fertilization process and thereafter every 2 min using a new in vitro culture system originally developed by the authors for time-lapse cinematography. We displayed 30 frames per second for analysis of the polyspermy block during fertilization. Three oocytes showed the leading and following sperm within the zona pellucida in the same microscopic field. The dynamic images obtained during the fertilization process using this new system revealed that once a leading sperm penetrated the zona pellucida and attached to the oocyte membrane, a following sperm was arrested from further penetration into the zona pellucida within 10 s. The present results strongly suggest the existence of a novel mechanism of polyspermy block that takes place at the zona pellucida immediately after fertilization. These findings are clearly different from previous mechanisms describing polyspermy block as the oocyte membrane block to sperm penetration and the zona reaction. The finding presented herein thus represents a novel discovery about the highly complicated polyspermy block mechanism occurring in human oocytes.

  10. Determination of the reactivity of cytotoxic immune cells with preimplantation mouse embryos

    SciTech Connect

    Ewoldsen, M.A.

    1987-01-01

    Cytotoxic immune cells were used in an assay, MELIA (mixed embryo leukocyte interaction assay) to test the ability of the cells to kill blastocyst stage embryos. The cytotoxic immune cells generated for use in this study, cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, and lymphokine activated killer (LAK) cells were shown to have phenotypic and cytolytic characteristics similar to those reported by other investigators. The lysis of the blastocysts in the MELIA was determined by measuring the inhibition of blastocoel retention and/or by the inhibition of incorporation of tritiated thymidine (/sup 3/H-TdR) into embryonic DNA. Blastocysts which possess ormore » lack their zonae pellucidae were tested to determine whether the zona pellucida plays an immunoprotective role in preimplantation development. The results indicated that CTLs only lysed embryonic cells when the zona pellucida was absent, but NK and LAK cells lysed embryonic cells whether the zona pellucida was present or absent. The results suggest that the zona pellucida may protect the preimplantation mouse embryo from lysis by CTLs but what protects the embryo from lysis by NK and LAK cells is unclear.« less

  11. Wave Attenuation on Muddy Bottoms - A Multidisciplinary Field Study Offshore Cassino Beach, Southern Brazil

    DTIC Science & Technology

    2005-09-30

    Rio de Janeiro Ocean Engineering Program / COPPE CP 68508 - Centro de Tecnologia - C203 21945-970 Rio de Janeiro , Brazil Phone/fax...NAME(S) AND ADDRESS(ES) Federal University of Rio de Janeiro ,Ocean Engineering Program / COPPE, CP 68508 - Centro de Tecnologia - C203,21945-970 Rio ...activities of the project: From Brazilian Institutions: 1- Federal University of Rio de Janeiro (UFRJ) / Laboratory for

  12. Latin American Security Challenges: A Collaborative Inquiry from North and South

    DTIC Science & Technology

    2004-01-01

    Jorge Eduardo Malena, and Gabriela E. Porn Conclusion 103 Bibliography 109 Abbreviations 115 Contributors 117 Collaborating Organizations 119 Titles...Malena of the Catholic University, Bue- nos Aires; and Gabriela E. Porn of the Centro de Estudios Hemisféricos on their chapter. With good fortune and...Malena of the Catholic University, Buenos Aires; and Gabriela E. Porn of the Centro de Estudios Hemisféricos assess “Chinese Interests in Latin

  13. Power Pack: U.S. Intervention in the Dominican Republic, 1965-1966 (Leavenworth Papers, Number 15)

    DTIC Science & Technology

    1988-07-01

    Center (Centro de Entrenamiento de las Fuerzas 22 Elias Wessin y Wessin Armadas, or CEFA), an elite group of nearly 2,000 specially trained infan- try...battalion combat team battalion landing team Centro de Entrenamiento de las Fuerzas Armadas (Armed Forces Training Cen- ter) Central Intelligence...operations. As with Lebanon in 1958, the Domin- ican intervention was a joint operation. In general terms, many of the de - ficiencies revealed in joint

  14. Aluminum Alloys Reinforced by Nano-Particles Dispersion

    DTIC Science & Technology

    2010-03-01

    C. Mendoza Ruiz, R. Martinez-Sanchez. Propiedades mecdnicas de compositos aluminio- nanotubos de carbono . Primer Encuentro Internacional ICM 2007...UNIT NUMBER N/A 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Centro de Investigacion en Materiales Avanzados, S.C. Miguel de Cervantes # 120...Principal Investigator: Dr. Roberto Martinez Sanchez Institution: Centro de Investigation en Materiales Avanzados, S.C. Address: Miguel de Cervantes

  15. Research Program In Tropical Infectious Diseases

    DTIC Science & Technology

    1991-12-15

    Central America at the base of the Yucatan Peninsula, surrounded on the west and north by Guatemala and Mexico and on the east by the Caribbean Sea...inferred that in Belize, 2 tropical infectious diseases are common. Yellow fever has been known to occur in the Yucatan ,1 dengue and malaria are...Centro Americano) representatives in Belize City. Two ERC technologists and two CML technicians attended an INCAP (Instituto de Nutricion de Centro

  16. Bibliography of Documents Related to the Theory, Operation, Performance and Applications of Coaxial Plasma Guns. Revision

    DTIC Science & Technology

    1990-11-01

    V., Grunberger, L. and Prior, W., "Observation of Solar Flare Type Processes in the Laboratory," in Solar Magnetic Fields, Symposium No. 43 of the...RF/FUS/84/6, Associazione EURATOM--Comitato Nazionale Energia Nucleare sulla Fusione, Centro di Frascati, Rome, Italy, September 1984. Brzosko, J. S...Energy Deuteron Beam Generation in Plasma Focus," Report No. 80.5, Associazione EURATOM--Comitato Nazionale Energia Nucleare sulla Fusione, Centro di

  17. Contributions of groundwater conditions to soil and water salinization

    NASA Astrophysics Data System (ADS)

    Salama, Ramsis B.; Otto, Claus J.; Fitzpatrick, Robert W.

    piézométrique. Le ou les ensembles salins se sont formés par évaporation couplée à la précipitation d'un carbonate alcalino-terreux et à la dissolution de sels par capillarité. Resumen La salinización es el proceso por el cual la concentración de sales disueltas en el agua o el suelo aumenta por causas naturales o antrópicas. El contenido de agua se reduce por uno o una combinación de los siguientes mecanismos: evaporación, evapotranspiración, hidrólisis y goteo entre acuíferos. La salinidad aumenta en la dirección del flujo subterráneo, desde los puntos de recarga hacia los de descarga. La salinización se puede explicar por dos modelos químicos que se pueden definir como erosión y deposición y que están de acuerdo con los clásicos procesos geológicos que tienen los mismos nombres. Se conocen cinco procesos de salinización de suelos ligados al agua o a la extracción de la misma en climas áridos o semiáridos. En todos los casos que se presentan el agua subterránea es el principal agente geológico de transmisión, acumulación y descarga de sales. A escala de pequeña cuenca, en Australia Occidental y del Sur, el agua se pierde mediante evapotranspiración e hidrólisis. El agua subterránea salina fluye a lo largo de los cauces de los arroyos y se acumula en los paleocanales, que actúan como depósitos, hasta que finalmente descarga en los lagos, donde se acumula la mayor parte de la salinidad. En los hummocks de la Zona Norte de los Grandes Llanos de Canadá y EEUU, la recarga localizada y el tipo de descarga provocan que la salinización tenga lugar fundamentalmente en las depresiones. A escala regional y en cuencas cerradas este mismo mecanismo provoca playas o lagos salinos. En los acuíferos continentales de los rift del Sudán, la salinidad se incrementa a lo largo de las líneas de flujo subterráneo y se concentra en el límite distal. La zona salina en cada rift forma una cresta cerrada que se manifiesta en los mapas piezom

  18. The behaviour of cow blastocyst in vitro: cinematographic and morphometric analysis.

    PubMed Central

    Massip, A; Mulnard, J; Vanderzwalmen, P; Hanzen, C; Ectors, F

    1982-01-01

    The behaviour of the cow blastocyst in vitro was studied by time-lapse cinematography and analysed by morphometry. Three types of behaviour were observed: continuous expansion followed by hatching; discontinuous expansion interrupted by few contractions and followed by hatching; discontinuous expansion interrupted by several rapid contractions without hatching. This demonstrated that the pulsatile activity of the blastocyst is not a necessary condition of hatching but also that only a moderate pulsatile activity is compatible with normal hatching. The time of hatching in vitro corresponded approximately with the time of zona loss in vivo (9-10 days). Rupture of the zona occurred at any point of the trophoblast layer. Hatching by herniation through a reduced opening of the zona was occasionally observed. The behavior of the embryos from a particular animal was very similar but differences were noted between embryos from different animals. Images Fig. 3 PMID:7076563

  19. [Polarized light microscopy for evaluation of oocytes as a prognostic factor in the evolution of a cycle in assisted reproduction].

    PubMed

    González-Ortega, C; Cancino-Villarreal, P; Alonzo-Torres, V E; Martínez-Robles, I; Pérez-Peña, E; Gutiérrez-Gutiérrez, A M

    2016-04-01

    Identification of the best embryos to transfer is a key element for success in assisted reproduction. In the last decade, several morphological criteria of oocytes and embryos were evaluated with regard to their potential for predicting embryo viability. The introduction of polarization light microscopy systems has allowed the visualization of the meiotic spindle and the different layers of the zona pellucida in human oocytes on the basis of birefringence in a non-destructive way. Conflicting results have been reported regarding the predictive value in ICSI cycles. To assess the predictive ability of meiotic spindle and zona pellucida of human oocytes to implant by polarized microscopy in ICSI cycles. Prospective and observational clinical study. 903 oocytes from 94 ICSI cycles were analyzed with polarized microscopy. Meiotic spindle visualization and zona pellucida birefringence values by polarized microscopy were correlated with ICSI cycles results. Meiotic spindle visualization and birefringence values of zona pellucida decreased in a direct basis with increasing age. In patients aged over the 35 years, the percentage of a visible spindle and mean zona pellucida birefringence was lower than in younger patients. Fertilization rate were higher in oocytes with visible meiotic spindle (81.3% vs. 64%; p < 0.0001), as well as embryo quality (47.4% vs. 39%; p=0.01). Fertilization rate was higher in oocytes with positive values of birefringence (77.5 % vs. 68.5% p=0.005) with similar embryo quality. Conception cycles showed oocytes with higher mean value of zona birefringence and visible spindle vs. no-conception cycles (p<0.05). Polarized light microscopy improves oocyte selection, which significantly impacts in the development of embryos with greater implantation potential. The use of polarized light microscopy with sperm selection methods, blastocyst culture and deferred embryo transfers will contribute to transfer fewer embryos without diminishing rates of live

  20. The Dammam aquifer in Bahrain - Hydrochemical characterization and alternatives for management of groundwater quality

    NASA Astrophysics Data System (ADS)

    Zubari, Waleed K.

    orientale. Quatre types de salinisation de l'eau souterraine ont été identifiés : une drainance verticale en provenance d'eaux saumâtres sous-jacentes dans les régions nord-centrales, ouest et est, une intrusion marine dans la région est, une intrusion d'eau de sebkha dans la région sud-ouest, et le retour d'irrigation dans un secteur localisé de la région sud-ouest. Quatre démarches alternatives de gestion de la qualité des eaux souterraines, acceptables par les services chargés de l'eau à Bahreïn, sont discutées et des zones d'action prioritaire sont proposées, selon le type et l'étendue de chacune des sources de salinisation et en fonction des usages de les eaux souterraines dans chaque secteur. L'efficacité des options de gestion proposées pour le contrôle de la dégradation de la qualité de l'aquifère de Damman devrait être ensuite évaluée au moyen d'un modèle de simulation. Resumen El acuífero de Damman, el más importante de Bahrein, se explota para usos agrícolas y domésticos. La sobreexplotación del mismo ha provocado su salinización por cuerpos de agua salada y salobre cercanos. Un estudio hidroquímico identificó las fuentes de salinización y ayudó a dibujar sus áreas de influencia. La calidad del agua subterránea se modifica significativamente en la dirección del flujo, desde el noroeste del país, donde las entradas son laterales y procedentes de Arabia Saudí, hacia el sur y el sudeste. Se han identificado cuatro tipos de salinización: flujo ascendente de agua salobre en las regiones norte-central, este y oeste; intrusión de agua marina en la región este; intrusión de agua salobre al sudoeste y, finalmente, retorno de riego en un área local de la región oeste. Se discuten cuatro alternativas para la gestión del agua y se proponen áreas prioritarias basadas en el tipo y la extensión de cada fuente de salinización y en los usos de agua subterránea en cada zona. La efectividad de las opciones de gestión propuestas

  1. In vitro studies on normal and pathological preimplantation development. I. Events of normal mouse preimplantation development as revealed by microcinematography.

    PubMed

    Checiu, M; Schlechta, B; Checiu, I; Sandor, S

    1990-01-01

    After briefly presenting the main historical data of in vitro culture of preimplantation mouse embryos and their filming, the first own observations on normal preimplantation development made by using microcinematography are presented: development from two-cell to eight-cell embryos; compaction and cavitation. The timing and the duration of various developmental events were recorded. Own observations were compared with previous cinematographic data reported by other authors. Some processes needing further investigations are evidenced: rotation within the zona pellucida, penetration of cytoplasmic emissions through the zona, contraction and reexpansion.

  2. Relation of runoff and soil erosion to weather types in the Mediterranean basin

    NASA Astrophysics Data System (ADS)

    Nadal-Romero, Estela; Peña-Angulo, Dhais

    2017-04-01

    Institute of Catelo Branco, School of Agriculture (ESACB), Portugal (10) Departamento de Agronomía, Universidad de Almería, Almería, Spain (11) Center for Research and Advanced Studies of the Nactional Polytechnic Institute, Mexico City, DF, Mexico (12) Centro Nacional de Supercomputacion, Department of Earth Sciences, Barcelona, Spain (13) Departamento de Ciencias de la Tierra, Universidad de Zaragoza, Zaragoza, Spain (14) Centro de Estudos de Recursos Naturais, Ambiente e Sociedade (CERNAS), Coimbra Agrarian Technical School, Polytechnic Institute of Coimbra, Coimbra, Portugal (15) Instituto de Agricultura Sostenible—CSIC, Córdoba, Spain (16) Grupo de Investigación GeoAmbiental (GIGA), Área de Geografía Física, Facultad de Filosofía y Letras. Universidad de Extremadura, Cáceres, Spain (17) Department of Waters, National Research Institute of Science and Technology for Environment and Agriculture, France (18) Department of Natural Resources Management and Agricultural Engineering, Agricultural University of Athens, Athens, Greece (19) Area of Physical Geography, Luis Vives Building, Luis de Ulloa Street, University of La Rioja, 26006 Logroño, Spain (20) Instituto Pirenaico de Ecología, IPE-CSIC, Zaragoza, Spain (21) Estación Experimental de Zonas Áridas, EEZA-CSIC, Almería, Spain (22) IRD, LISAH, Montpellier, France (23) Department of Agri-Food and Environmental Systems Management, University of Catania, Catania, Italy (24) Institute of Earth and Environmental Science, University of Potsdam, Potsdam, Germany (25) Center for Applied Geosciences. Faculty of Science, Eberhard Karls Universität Tübingen, Germany (26) Departamento de Geología y Geoquímica, Universidad Autónoma Madrid, Madrid, Spain (27) Departamento de Geografía, Universidad de Salamanca, Salamanca, Spain (28) Departamento de Geografía, Grupo de Investigación Geografía Física y Territorio—RNM279, Andalucía Tech, Universidad de Málaga, Málaga, Spain (29) Grup de Recerca Ambiental

  3. PREFACE: International Symposium on Ultrasound in the Control of Industrial Processes (UCIP 2012)

    NASA Astrophysics Data System (ADS)

    Segura, Luis Elvira; Resa López, Pablo; Salazar, Jordi; Benedito Fort, José Javier; Martínez Graullera, Óscar

    2012-12-01

    The following describes most of the presentations (both oral and poster) given at the International Symposium of Ultrasound in the Control of Industrial Processes (UCIP 2012) celebrated in Madrid between 18 and 20 April 2012. This event was intended to be a meeting point for scientists, engineers and professionals from all over the world in the field of ultrasonics applied to the characterization and control of materials and processes in the industry. More precisely, the topics included were: 1. Novel applications of ultrasound in the industry (including high-power ultrasound) Food science Biotechnology and microbiology Pharmaceutics and cosmetics Petrochemistry and civil engineering 2. New insights in the ultrasonic characterization of media: Fluids and emulsions Nano- and micro-particle dispersions Soft materials Porous bodies and inhomogeneous materials 3. New developments in ultrasonic measuring techniques: Acoustic microscopy Piezoelectric sensors Ultrasonic imaging Signal processing The symposium was organized by the Centro de Acústica Aplicada y Evaluación No Destructiva (CAEND, UPM-CSIC) in collaboration with the Universidad Politécnica de Cataluña, the Universidad Politécnica de Valencia and the University of Leeds. During the conference, 32 posters and 33 oral communications were presented. In addition, 4 invited lectures were imparted: 'Acoustic microscopy, spectroscopy and nanoparticle detection' by Dr Malcolm Povey; 'Acoustic and electroacoustic spectroscopy' by Dr Andrei Dukhin; 'High-Resolution Ultrasonic Spectroscopy and its application for material analysis by Dr Vitaly Buckin; 'Ultrasonic sensors for process applications - state of the art' by Dr Bern Henning; and three tutorials were given: 'PZFlex - Finite Element Analysis for Virtual Prototyping' by Weidlinger Associates; 'SITAU - A flexible architecture controlled by MATLAB for the development of ultrasonic applications' by DASEL; 'Ultra-SCATTERERTM (Acoustics Suite) - The R&D Tool for

  4. Prevalence of self-medication in Brazil and associated factors.

    PubMed

    Arrais, Paulo Sérgio Dourado; Fernandes, Maria Eneida Porto; Pizzol, Tatiane da Silva Dal; Ramos, Luiz Roberto; Mengue, Sotero Serrate; Luiza, Vera Lucia; Tavares, Noemia Urruth Leão; Farias, Mareni Rocha; Oliveira, Maria Auxiliadora; Bertoldi, Andréa Dâmaso

    2016-12-01

    ), coletados de setembro de 2013 a fevereiro de 2014, por meio de entrevistas em domicílio. Todas as pessoas que referiram usar qualquer medicamento sem prescrição por médico ou dentista foram classificadas como praticantes de automedicação. Foram calculadas razões de prevalência bruta e ajustada (regressão de Poisson) e seus respectivos intervalos de confiança de 95% na investigação dos fatores associados ao consumo de medicamentos por automedicação. As variáveis independentes foram: aspectos sociodemográficos, de condições de saúde e de acesso e utilização de serviços de saúde. Adicionalmente, foram identificados os medicamentos mais consumidos por automedicação. A prevalência da automedicação no Brasil foi de 16,1% (IC95% 15,0-17,5), sendo maior na região Nordeste (23,8%; IC95% 21,6-26,2). Após análise ajustada, automedicação mostrou-se associada a ser do sexo feminino, pertencer às faixas etárias 10-19 anos, 20-29 anos, 40-59 anos e 60 anos ou mais, residir na região Norte, Nordeste ou Centro-Oeste, e ter uma ou duas ou mais doenças crônicas. Os analgésicos e os relaxantes musculares foram os grupos terapêuticos mais utilizados por automedicação, sendo a dipirona o fármaco mais consumido. No geral, a maioria dos medicamentos usados por automedicação foram classificados como isentos de prescrição (65,5%). A automedicação é prática corrente no Brasil e envolve, principalmente, o uso de medicamentos isentos de prescrição, devendo os usuários ficarem atentos aos seus possíveis riscos.

  5. CURRENT SITUATION OF MEDICINE III AND CHALLENGES.

    PubMed

    Ferreira, Lydia Masako

    2015-01-01

    tradition and history. For the maintenance and advancement of the area some challenges and goals were established to be developed in the period from 2013 to 2016. Descrever a situação atual da área Medicina III da Capes e detectar os desafios para o próximo quadriênio de avaliação. Os documentos e relatórios de reuniões de área a partir de 2004 até 2013 da Medicina III da Capes foram lidos assim como os relatórios e as fichas de avaliação de cada Programa de Pós Graduação (PPG) da área e a subpágina da área no site da Capes. Os dados referentes ao Processo de Avaliação, à Ficha de Avaliação e aos dados do Corpo Docente, Discente e Produção científica de todos os PPG da Medicina III foram computados e analisados. A partir desses dados foram detectados os desafios da área para o próximo quadriênio (2013-2016). Dentre os 3.806 PPG, a Medicina III apresentava 41 PPG durante última avaliação trienal e evoluiu de 18% para 43% de PPG > conceito muito bom (Triênios 2001-2003 e 2010-2012). A maioria dos PPG se localizavam na Região Sudeste (32), três no Sul e dois no Nordeste. Não havia nenhum PPG no Norte ou Centro-Oeste. Em 2013 e 2014 foram aprovadas quatro mestrados profissionais e um mestrado e doutorado acadêmico. A média de docente permanente no triênio foi de 558 docentes com cerca de três alunos titulados/docente. O número de doutores titulados tem aumentado assim como a razão Doutorado/Mestrado. A proporção de publicações em periódicos de maior impacto (A1, A2, B1 e B2) saltou de 30% para 50% demonstrando resposta positiva da comunidade à política da área. Os desafios detectados foram: diminuir assimetria regional, elevar o número de mestres e doutores de excelência, reavaliação das revistas nacionais, estimular e definir indicadores de internacionalização, inclusão de pós-doutores e definição de seus indicadores, análise da nucleação do PPG, PPG 3x3, inclusão ensino médio e fundamental, mestrado

  6. Cardiovascular Risk Stratification and Statin Eligibility Based on the Brazilian vs. North American Guidelines on Blood Cholesterol Management.

    PubMed

    Cesena, Fernando Henpin Yue; Laurinavicius, Antonio Gabriele; Valente, Viviane A; Conceição, Raquel D; Santos, Raul D; Bittencourt, Marcio S

    2017-06-01

    íduos saudáveis em prevenção primária: avaliar a relação entre o risco cardiovascular segundo a V Diretriz Brasileira de Dislipidemias e o risco calculado pelas pooled cohort equations (PCE); comparar a proporção de indivíduos elegíveis para estatinas, de acordo com diferentes critérios. Em indivíduos de 40 a 75 anos submetidos consecutivamente a avaliação rotineira de saúde em um único centro, quatro critérios de elegibilidade para estatina foram definidos: BR-1, BR-2 (LDL-c acima ou pelo menos 30 mg/dL acima da meta preconizada pela diretriz brasileira, respectivamente), EUA-1 e EUA-2 (risco estimado pelas PCE em 10 anos ≥ 5,0% ou ≥ 7,5%, respectivamente). Foram estudados 13.947 indivíduos (48 ± 6 anos, 71% homens). A maioria dos indivíduos de risco intermediário ou alto pela V Diretriz apresentou risco calculado pelas PCE baixo e mais de 70% daqueles considerados de alto risco o foram devido à presença de fator agravante. Foram elegíveis para estatina 24%, 17%, 4% e 2% das mulheres pelos critérios BR-1, BR-2, EUA-1 e EUA-2, respectivamente (p < 0,01). Os respectivos valores para os homens foram 75%, 58%, 31% e 17% (p < 0,01). Oitenta e cinco por cento das mulheres e 60% dos homens elegíveis para estatina pelo critério BR-1 não seriam candidatos pelo critério EUA-1. Comparada à diretriz norte-americana, a V Diretriz Brasileira considera uma proporção substancialmente maior da população como elegível para estatina em prevenção primária. Isso se relaciona com discrepâncias entre o risco estratificado pela diretriz brasileira e o calculado pelas PCE, particularmente devido à reclassificação de risco baseada em fatores agravantes.

  7. Radiocarbon dating of dissolved inorganic carbon in groundwater from confined parts of the Upper Floridan aquifer, Florida, USA

    NASA Astrophysics Data System (ADS)

    Plummer, Niel; Sprinkle, Craig

    2001-03-01

    édolomitisation), (2) la réduction des sulfates accompagnant une dégradation microbienne du carbone organique, (3) la recristallisation de la calcite (échange isotopique), et (4) le mélange d'eau douce avec de l'eau salée, jusqu'à 7%, dans certaines zones côtières. Les transferts minéraux nets calculés sont extrêmement faibles dans les parties situées dans l'amont de l'aquifère ils augmentent significativement dans les zones de l'aval, montrant en partie au moins l'existence d'une drainance ascendante depuis l'aquifère inférieur de Floride et une circulation qui met en relation les unités captives du système aquifère de Floride. Les âges radiocarbone corrigés sont indépendants des trajets d'écoulement et représentent des temps de transit de l'eau depuis la zone de recharge vers le point de prélèvement dans l'aquifère. En aval de Polk City (âge corrigé 1,7 ka) et de Keystone Heighats (âge corrigé 0,4 ka), 14 des 22 échantillons d'eau présentent des âges corrigés compris entre 20 et 30 ka, ce qui montre que la plus grande partie des ressources actuelles en eau douce de la nappe supérieure de Floride provient d'une recharge effectuée au cours de la dernière période glaciaire. Toutes ces eaux anciennes sont enrichies en 18O et en 2H par rapport à l'infiltration actuelle, avec un enrichissement maximal de δ18O d'environ 2.0‰. Resumen. Se han evaluado varios modelos geoquímicos con el fin de mejorar la datación del carbono inorgánico disuelto (CID) en las aguas subterráneas de las zonas confinadas del acuífero Superior de Florida, que ocupa el centro y nordeste de Florida (Estados Unidos). Las reacciones geoquímicas dominantes en cuanto a la actividad del 14C del CID incluyen: (1) disolución de dolomita y anhidrita, con precipitación de calcita (o de-dolomitización), (2) reducción de sulfato, acompañada por degradación microbiana de carbón orgánico, (3) recristalización de calcita (intercambio isotópico), y (4) mezcla de agua dulce

  8. Unidades del paisaje de Puerto Rico: la influencia del clima, el substrato y la topografia

    Treesearch

    William Gould; Michael E. Jimenez; Gary Potts; Maya Quinones; Sebastian Martinuzzi

    2008-01-01

    El mapa de unidades del paisaje de Puerto Rico representa variaciones climaticas, topograficas y del substrato mediante la integracion de seis zonas climaticas (Ewel y Whitmore, 1973), seis substratos (Bawiec, 2001; USGS, 2005), cinco posiciones topograficas, o topoformas (Martinuzzi et al. 2007), y cuerpos de agua (USGS 2005). Los substratos representan el conjunto...

  9. Function and culture requirements of snow leopard (Panthera uncia) spermatozoa in vitro.

    PubMed

    Roth, T L; Howard, J G; Donoghue, A M; Swanson, W F; Wildt, D E

    1994-08-01

    Electroejaculates from eight snow leopards were used to determine how the motility of spermatozoa was influenced by (i) type of media (Ham's F10, PBS, human tubal fluid or RPMI-1640); (ii) holding temperature (23 degrees C versus 37 degrees C); (iii) washing of spermatozoa and (iv) a sperm metabolic enhancer, pentoxifylline. The duration of sperm motility was assessed by evaluating samples in each treatment every hour for 6 h and a sperm motility index (a value combining percentage sperm motility and rate of forward progression) calculated. Spermatozoa from the Ham's F10, PBS and PBS plus pentoxifylline treatments were also co-incubated with zona-intact, domestic cat eggs that were fixed and evaluated for spermatozoa bound to the zona pellucida, penetrating the outer and inner layers of the zona pellucida and within the perivitelline space. During the 6 h co-incubation, the sperm motility index in PBS with pentoxifylline was greater (P < 0.05) than in PBS alone which, in turn, was greater (P < 0.05) than in the other three test media. Washing the spermatozoa enhanced (P < 0.05) motility in both PBS and PBS plus pentoxifylline relative to unwashed samples, but there was no effect (P > 0.05) of holding temperature. Pentoxifylline supplementation enhanced (P < 0.05) the proportion of cat eggs with bound, but not penetrated, snow leopard spermatozoa in the inner layer of the zona pellucida, and there were no spermatozoa in the perivitelline space.(ABSTRACT TRUNCATED AT 250 WORDS)

  10. Degradación de Marismas Costera: el Impacto de la Eutrofización en la Supervivencia del las Marismas Costeras en New England y Central California, USA. (Salt Marsh Deterioration in New England and Central California: Impacts of Eutrophication on Salt Marsh Survival.)

    EPA Science Inventory

    Las marismas costeras proporcionan numerosos beneficios a los ecosistemas, incluyendo la proporción del hábitat de la flora y fauna, la protección de las zonas costeras contra inundaciones durante eventos extremos, mejoran la calidad del agua para las almejas y ostras a través de...

  11. 76 FR 58562 - Additional Designations, Foreign Narcotics Kingpin Designation Act

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-21

    ... controlled by significant foreign narcotics traffickers as identified by the President. In addition, the..., Jalisco, Mexico; Plaza Del Sol Local 28, Zona R, Guadalajara, Jalisco, Mexico; Paseo Del Heliotropo 3426, Monraz, Guadalajara, Jalisco, Mexico; DOB 09 Aug 1955; POB Jalisco, Mexico; Citizen Mexico; Nationality...

  12. Autoradiographic visualization of the mouse egg's sperm receptor bound to sperm

    SciTech Connect

    Bleil, J.D.; Wassarman, P.M.

    1986-04-01

    The extracellular coat, or zona pellucida, of mammalian eggs contains species-specific receptors to which sperm bind as a prelude to fertilization. In mice, ZP3, one of only three zona pellucida glycoproteins, serves as sperm receptor. Acrosome-intact, but not acrosome-reacted, mouse sperm recognize and interact with specific O-linked oligosaccharides of ZP3 resulting in sperm-egg binding. Binding, in turn, causes sperm to undergo the acrosome reaction; a membrane fusion event that results in loss of plasma membrane at the anterior region of the head and exposure of inner acrosomal membrane with its associated acrosomal contents. Bound, acrosome-reacted sperm are able to penetratemore » the zona pellucida and fuse with the egg's plasma membrane (fertilization). In the present report, we examined binding of radioiodinated, purified, egg ZP3 to both acrosome intact and acrosome reacted sperm by whole-mount autoradiography. Silver grains due to bound 125I-ZP3 were found localized to the acrosomal cap region of heads of acrosome-reacted sperm. Under the same conditions, 125I-fetuin bound at only background levels to heads of both acrosome-intact and -reacted sperm, and 125I-ZP2, another zona pellucida glycoprotein, bound preferentially to acrosome-reacted sperm. These results provide visual evidence that ZP3 binds preferentially and specifically to heads of acrosome intact sperm; properties expected of the mouse egg's sperm receptor.« less

  13. ¿Qué está haciendo la EPA para proteger a los arrecifes de coral?

    EPA Pesticide Factsheets

    La EPA protege a los arrecifes de coral mediante la implementación de programas de la Ley de Agua Limpia que protegen la calidad del agua en cuencas y zonas costeras de las áreas con arrecifes de coral.

  14. 9 CFR 98.17 - Procedures.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., and sterilization. (1) All equipment that comes in contact with embryos or with media used for their... found to be intact and free from any adherent material. (5) After washing and examination of the zona... be moved to a U.S. Department of Agriculture-operated animal import center in either New York, Hawaii...

  15. 9 CFR 98.17 - Procedures.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., and sterilization. (1) All equipment that comes in contact with embryos or with media used for their... found to be intact and free from any adherent material. (5) After washing and examination of the zona... be moved to a U.S. Department of Agriculture-operated animal import center in either New York, Hawaii...

  16. Army Installations Water Sustainability Assessment: An Evaluation of Vulnerability to Water Supply

    DTIC Science & Technology

    2009-09-01

    del Norte Water Task Force PM particulate matter PNNL Pacific Northwest National Laboratory PWC Public Works Commission PWD RFTA RSC Regional...ct io n E n gi n ee ri n g R es ea rc h L ab or at or y Approved for public release; distribution is unlimited. ERDC/CERL TR-09-38...and Ryan Holmes University of Illinois at Urbana-Champaign Final Report Approved for public release; distribution is unlimited. Prepared for U.S

  17. Inventorying and Monitoring of Tropical Dry Forests Tree Diversity in Jalisco, Mexico Using a Geographical Information System

    Treesearch

    Efren Hernandez-Alvarez; Dieter R. Pelz; Carlos Rodriguez Franco

    2006-01-01

    Tropical dry forests in Mexico are an outstanding natural resource, due to the large surface area they cover. This ecosystem can be found from Baja California Norte to Chiapas on the eastern coast of the country. On the Gulf of Mexico side it grows from Tamaulipas to Yucatan. This is an ecosystem that is home to a wide diversity of plants, which include 114 tree...

  18. Phylogenetic and morphologic evidence confirm the presence of a new montane cloud forest associated bird species in Mexico, the Mountain Elaenia (Elaenia frantzii; Aves: Passeriformes: Tyrannidae)

    PubMed Central

    Ortiz-Ramírez, Marco F.; Ríos-Muñoz, César A.; Cayetano-Rosas, Héctor; Bowie, Rauri C. K.; Navarro-Sigüenza, Adolfo G.

    2016-01-01

    Here we provide evidence to support an extension of the recognized distributional range of the Mountain Elaenia (Elaenia frantzii) to include southern Mexico. We collected two specimens in breeding condition in northwestern Sierra Norte de Chiapas, Mexico. Morphologic and genetic evidence support their identity as Elaenia frantzii. We compared environmental parameters of records across the entire geographic range of the species to those at the northern Chiapas survey site and found no climatic differences among localities. PMID:26855860

  19. Biology of the Rio Grande border region : a bibliography

    USGS Publications Warehouse

    Johnson, Lynne E.; Jacobs, Linda J.; Papoulias, Diana

    1997-01-01

    This bibliography includes 1,913 references to the literature of the Rio Grande (Rio Bravo del Norte). The specific geographic area covered extends 100 km on either side of the river from Elephant Butte Dam in New Mexico to the Gulf of Mexico. The bibliography focuses on the biological literature, divided into major subject areas, and also includes supporting literature from the physical and environmental sciences.

  20. Use by bats of old-growth redwood hollows on the north coast of California

    Treesearch

    Steven T. Gellman; William J. Zielinski

    1996-01-01

    Use by bats of basal hollows in old-growth redwoods was indexed using the weight of guano collected on water-permeable screens (guano traps) installed inside the trees. Twenty-six trees were systematically selected from a stand that flanks both sides of an interstate highway in Del Norte Co., California. Traps were checked for guano once a month for 18 months. The...

  1. Field Trial of Attenuated Salmonella Typhi Live Oral Vaccine TY21A in Liquid and Enteric-Coated Formulations and Epidemiological Survey for Incidence of Diarrhea due to Shigella Species

    DTIC Science & Technology

    1989-03-01

    and absolute efficacy of three doses of Ty2la vaccine given in enteric-coated capsule or liquid formulation. Intensive clinical and bacteriologic...TABLES Table 1. Evaluation of the efficacy of three doses of the enteric-coated capsule formulation of Ty2la live oral vaccine given within one week in...November, 1986 thzough February, 1989 of a field trial in Area Sur Oriente and Area Norte assessing the efficacy of Ty21a vaccine in liquid or enteric

  2. Field Trial of Attentuated Salmonella Typhi Live Oral Vaccine Ty21a in Liquid and Enteric-Coated Formulations and Epidemiological Survey for Incidence of Diarrhea due to Shigella Species

    DTIC Science & Technology

    1988-05-18

    trial was initiated in Area Sur Oriente and Area Norte, Santiago, Chile,. to compare the relative and absolute’ efficacy of three doses of Ty2la vaccine ...neighboirhood of Area Oriente, Santiago with the broad objective of pr3paring a field area uhere the efficacy of vaccines against Shigella...The high incidence rates of Shisella, ETEC, and EPEC infection make Santa Julia a suitable place for testing the efficacy of vaccines against these

  3. Patterns of Global Terrorism: 1997.

    DTIC Science & Technology

    1998-04-01

    four Colombian Coca - Cola employees at a roadblock in Norte de Santander. The rebels seek individual ransoms and a payoff from Coca - Cola to prevent...further kidnappings and have approached other Coca - Cola officials demanding a protection payoff. 23 December Pakistan Unidentified assailants...economic sanctions on Sudan for its sponsorship of international terrorism, its efforts to destabilize neighboring countries, and its abysmal human

  4. CTIO History | CTIO

    Science.gov Websites

    Preserving the Dark Skies La Oficina de Protección de la Calidad del Cielo del Norte de Chile - OPCC Light Pollution StarLight Universe The World at Night (TWAN) International Dark-Sky Association (IDA) Students REU ‹› You are here CTIO Home » About » CTIO History CTIO History Brief History of THE CERRO

  5. World Epidemiology Review, Number 90

    DTIC Science & Technology

    1978-01-12

    Bom Jesus and Aurora do Norte. This was reported to Agriculture Minister Alysson Paulinelli in Brasilia by Antonio Augusto Azeredo Coutinho, the...municipios of Dianopolis, Ponte Alta de Bom Jesus , Almas and Alvorada to observe the situation. He confirmed that the rabies outbreak has affected...department has established a system of epidemiological vigilance, which consists of receiving, every 7 days, information from all municipal hospitals

  6. A Numerical Study of the Plata River Plume Along the Southeastern South American Continental Shelf

    DTIC Science & Technology

    2005-01-01

    Aires Depto. de Oceanografia, Servicio de Hidrografia Naval and Depto. CiEncias de la Atm6sfera y los Ocdanos, Buenos Aires, Argentina ABSTRACT The...descarrega no oceano Aguas de uma bacia de drenagem que cobre uma ampla Area da Amdrica do Sul. Sua pluma extende-se ao longo do norte da Argentina ...Instituto Nacional de Investigaci6n y Orlanski, I. 1976. A simple boundary condition for Desarrollo Pesquero, 109pp. unbounded hyperbolic flows. J

  7. The Challenge of Violent Drug-Trafficking Organizations: An Assessment of Mexican Security Based on Existing RAND Research on Urban Unrest, Insurgency, and Defense-Sector Reform

    DTIC Science & Technology

    2011-01-01

    Bad Factors Outcome Afghanistan (post-Soviet) 0 10 –10 Loss Somalia 1 10 –9 Loss Chechnya I 2 10 –8 Loss Rwanda 2 10 –8 Loss Zaire (anti-Mobutu) 0...most of Baha California (Norte/Sur), Sonora, Chihuahua, Durango, Sinaloa, Nayarit, Jalisco, Colima, Quintana Roo, Yucatan, Campeche, Zacatecas...Sources of Success in Counterinsurgency and Victory Has a Thousand Fathers: Detailed Counterinsurgency Case Stud- ies . For that study, the research

  8. JPRS Report, Science & Technology, Europe & Latin America, Brazil: Selections from the 1987 Federal S&T Budget.

    DTIC Science & Technology

    1988-02-24

    of Goias 67 . Federal University of the State of Rio de Janeiro 68 . Federal University of Juiz de Fora 68 . Federal University of Minas Gerais...Federal University of Rio Grande do Norte 72 . Federal University of Rio Grande do Sul • 72 . Federal University of Rio de Janeiro 73...University of Rio de Janeiro 75 . School of Agrarian Sciences of Para 76 . School of Medicine of the Minas Triangle 77 . Federal Technological Education

  9. A new six-pored Amphisbaena (Squamata: Amphisbaenidae) from the coastal zone of northeast Brazil.

    PubMed

    Roberto, Igor Joventino; Brito, Lucas B M; Avila, Robson W

    2014-01-06

    We describe a new Amphisbaena from the Brazilian coastal zone at the municipalities of Guamaré and Macau, state of Rio Grande do Norte. The new species, Amphisbaena littoralis sp. nov., is characterized by six precloacal pores, 252-264 body annuli, 30-34 tail annuli with autotomy on the 6th tail annuli, 20-22 dorsal and 21-24 ventral segments to the midbody annulus.

  10. Southeast Asia Report

    DTIC Science & Technology

    1987-03-25

    Karya, directorate general, in the introduction of processing a system to keep water free of bacterias by benefiting the power of the sunshine. A...Kalinga-Apayao, Quirino, Quezon, Nueva Ecija, Albay, Mindoro Oriental, Aklan, Lanao del Norte and Davao. The biggest clash was in Mandog, Davao...Isabela. One insurgent was killed in a clash with NPA rebels in Kabugao, Kalinga-Apayao; and other casualties were in Quirino; Nueva Ecija; Sariyaya

  11. 76 FR 64995 - Endangered and Threatened Wildlife and Plants; Designation of Revised Critical Habitat for the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-19

    ...We, the U.S. Fish and Wildlife Service (Service), propose to revise critical habitat for the tidewater goby (Eucyclogobius newberryi) under the Endangered Species Act of 1973, as amended (Act). In total, approximately 12,157 acres (4,920 hectares) are being proposed for designation as critical habitat. The proposed revised critical habitat is located in Del Norte, Humboldt, Mendocino, Sonoma, Marin, San Mateo, Santa Cruz, Monterey, San Luis Obispo, Santa Barbara, Ventura, Los Angeles, Orange, and San Diego Counties, California.

  12. 78 FR 8745 - Endangered and Threatened Wildlife and Plants; Designation of Critical Habitat for Tidewater Goby

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-06

    ...We, the U.S. Fish and Wildlife Service, designate critical habitat for the tidewater goby (Eucyclogobius newberryi) under the Endangered Species Act of 1973, as amended (Act). In total, approximately 12,156 acres (4,920 hectares) in Del Norte, Humboldt, Mendocino, Sonoma, Marin, San Mateo, Santa Cruz, Monterey, San Luis Obispo, Santa Barbara, Ventura, Los Angeles, Orange, and San Diego Counties, California, fall within the boundaries of the critical habitat designation.

  13. Translations on Narcotics and Dangerous Drugs No. 298

    DTIC Science & Technology

    1977-05-06

    Cabecao was arrested, and detectives Dagomar Ruas Silva and Vitor Alves da Mota, both of whom were working at the time at the now-closed Vigilancia-Norte...Carvajal. It is believed that Mario Gil Ramirez , husband of Bercey Espinosa de Gil, and Miguel Alberto Lopez, who was recently arrested by F-2, are...clandestine laboratory in that city. Arrested were Segundo Gonzaga Granda, Ocamidoro Gonzaga Diaz and Epaminondas Ramirez Acosta. The traffickers were

  14. Preliminary Observations on the Changing Roles of Malaria Vectors in Southern Belize

    DTIC Science & Technology

    1993-01-01

    darlingi (Diptera: Cu- licidae) de la Ceiba, Atlantida, Honduras. Thesis. Maestria en Entomologia. Universidad de Panama, Panama City, Panama. 456...Brown and C. Cordon-Rosales. 1992. Potential malaria vectors in northern Guatemala (Vectores potenciales de ma- laria in la region norte de Guatemala...Serra de Aqua in June 1946 (Linthicum 1988). We initiated a malaria vector research pro- gram in Belize in 1990 and conducted extensive larval

  15. You can see galaxies from your computer | CTIO

    Science.gov Websites

    Calendar Activities NOAO-S EPO Programs CADIAS Astro Chile Hugo E. Schwarz Telescope Dark Sky Education Preserving the Dark Skies La Oficina de Protección de la Calidad del Cielo del Norte de Chile - OPCC Light Pollution StarLight Universe The World at Night (TWAN) International Dark-Sky Association (IDA) Students REU

  16. 55. E.M.C., photographer July 3, 1936 HUMBOLDT COUNTY, SECTION J, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    55. E.M.C., photographer July 3, 1936 HUMBOLDT COUNTY, SECTION J, HIGHWAY 1. 1-HUM-I-J #80, BITUMULS PORTION OF EXPERIMENTAL SECTION, E.M.C, 7-3-36. BACK READS: C/PROCESSING STABILIZING BITUMULS WITH BLADE AFTER BEING PROCESSED WITH SPRING TOOTH HARROW. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

  17. Registros del Cuco Hormiguero (Neomorphus geoffroyi) en la Reserva de Biosfera de Bosawas, Nicaragua

    Treesearch

    H.M. Herrera-Rosales; M. Tórrez; W.J. Arendt

    2014-01-01

    El cuco hormiguero, Neomorphus geoffroyi, se registró en la Reserva de Biósfera de Bosawás y representa el primer gráfico de la especie para el Norte y para el país. Esta especie es rara y es sensible a la deforestación, se observó en compañía de bandadas mixtas, con su comportamiento...

  18. Microheater as an alternative to lasers for in-vitro fertilization applications

    NASA Astrophysics Data System (ADS)

    Palanker, Daniel V.; Turovets, Igor; Glazer, Rima; Reubinoff, Benjamin E.; Hilman, Dalia; Lewis, Aaron

    1999-06-01

    During the last decade various lasers have been applied to drilling of the micrometer-sized holes in the zona pellucida of oocytes for in-vitro fertilization applications. In this paper we describe an alternative approach to laser instrumentation based on microfabricated device capable of precise drilling of uniform holes in the zona pellucida of oocytes. This device consists of a thin (1 micrometer) film microheater built on the tip of glass capillary with a diameter varying between a few to a few tens of micrometers. Duration of the pulse of heat produced by this microheater determines the spatial confinement of the heat wave in the surrounding liquid medium. We have demonstrated that gradual microdrilling of the zona pellucida can be accomplished using a series of pulses with duration of about 300 microseconds when the microheater was held in contact with the zona pellucida. Pulse energy applied to 20 micrometer tip was about 4 (mu) J. In vitro development and hatching of 127 micromanipulated embryos was compared to 103 non-drilled control embryos. The technique was found to be highly efficient in creating round, uniform, well defined holes with a smooth wall surface, matching the size of the heating source. The architecture of the surrounding zona pellucida was unaffected by the drilling, as demonstrated by scanning electron microscopy. Micromanipulated embryos presented no signs of thermal damage under light microscopy. The rate of blastocyst formation and hatching was similar in the micromanipulated and control groups. Following further testing in animal models, this methodology may be used as a cost- effective alternative to laser-based instrumentation in clinical applications such as assisted hatching and embryo biopsy.

  19. Culture of bovine embryos on a polydimethylsiloxane (PDMS) microwell plate.

    PubMed

    Akagi, Satoshi; Hosoe, Misa; Matsukawa, Kazutsugu; Ichikawa, Akihiko; Tanikawa, Tamio; Takahashi, Seiya

    2010-08-01

    We fabricated a polydimethylsiloxane (PDMS)-based microwell plate (PDMS-MP) containing 100 microwells with a rounded bottom and examined whether it can be used for culture of individual in vitro fertilized (IVF) embryos or parthenogenetically activated zona-free embryos in cattle. In Experiment 1, we examined the in vitro developmental ability of IVF embryos cultured individually on PDMS-MP. After IVF, 20 embryos were transferred into 100 microl drops on PDMS-MP and cultured individually in each well of PDMS-MP (PDMS group). After 7 days of culture, the embryos in the PDMS group developed to the blastocyst stage at the same rate of those in the control group cultured in a group of 20 embryos without PDMS-MP. There were no differences in total number of cells and the ratio of inner cell mass to total cells between the PDMS and control groups. In Experiment 2, we examined the in vitro developmental ability of parthenogenetically activated zona-free bovine embryos cultured individually on PDMS-MP. The zona-free embryos were cultured individually in each well of a PDMS-MP or in each well produced by pressing a darning needle onto the bottom of a culture dish (WOW group). After 7 days of culture, the blastocyst formation rate and cell number of blastocysts in the PDMS group did not differ from those of the zona-intact embryos in the control group. Also, there were no differences in the blastocyst formation rate and cell number of blastocysts between the WOW and PDMS groups. These results suggest that the culture system using PDMS-MP is useful for individual embryos or zona-free embryos in cattle.

  20. The Gran Telescopio Canarias and Calar Alto Virtual Observatory Compliant Archives

    NASA Astrophysics Data System (ADS)

    Alacid, J. M.; Solano, E.; Jiménez-Esteban, F. M.; Velasco, A.

    2014-05-01

    The Gran Telescopio Canarias and Calar Alto archives are the result of the collaboration agreements between the Centro de Astrobiología and two entities: GRANTECAN S.A. and the Centro Astronómico Hispano Alemán (CAHA). The archives have been developed in the framework of the Spanish Virtual Observatory and are maintained by the Data Archive Unit at Centro de Astrobiología. The archives contain both raw and science ready data and have been designed in compliance with the standards defined by the International Virtual Observatory Alliance, which guarantees a high level of data accessibility and handling. In this paper we describe the main characteristics and functionalities of both archives.