EnviroAtlas - Memphis, TN - Ecosystem Services by Block Group

EPA Pesticide Factsheets

This EnviroAtlas dataset presents environmental benefits of the urban forest in 703 block groups in Memphis, Tennessee. Carbon attributes, temperature reduction, pollution removal and value, and runoff effects are calculated for each block group using i-Tree models (www.itreetools.org), local weather data, pollution data, EPA provided city boundary and land cover data, and U.S. Census derived block group boundary data. This dataset was produced by the US Forest Service to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

76 FR 31885 - Prevailing Rate Systems; Redefinition of the Northern Mississippi and Memphis, TN, Appropriated...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-02

... OFFICE OF PERSONNEL MANAGEMENT 5 CFR Part 532 RIN 3206-AM37 Prevailing Rate Systems; Redefinition... Prevailing Rate Advisory Committee (FPRAC) to best match the county proposed for redefinition to a nearby FWS... Memphis wage area. The Federal Prevailing Rate Advisory Committee (FPRAC), the national labor-management...

Youth Violence: Developing Local and State Solutions. Hearings before the Subcommittee on Youth Violence of the Committee on the Judiciary. United States Senate, One Hundred Fourth Congress, Second Session on Focusing on Youth Violence and Developing Local and State Solutions (Memphis and Nashville, TN, February 15 and 16, 1996).

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. Senate Committee on the Judiciary.

This hearing focused on youth violence and the importance of developing local and state solutions. Senator Fred Thompson made an introductory statement. This was followed by four panels on each of 2 days of testimony. Day one's first panel included involved students and business people from Memphis, TN. The second panel included two Tennessee…

Racial disparities in survival outcomes by breast tumor subtype among African American women in Memphis, Tennessee.

PubMed

Vidal, Gregory; Bursac, Zoran; Miranda-Carboni, Gustavo; White-Means, Shelley; Starlard-Davenport, Athena

2017-07-01

Racial disparities in survival among African American (AA) women in the United States have been well documented. Breast cancer mortality rates among AA women is higher in Memphis, Tennessee as compared to 49 of the largest US cities. In this study, we investigated the extent to which racial/ethnic disparities in survival outcomes among Memphis women are attributed to differences in breast tumor subtype and treatment outcomes. A total of 3527 patients diagnosed with stage I-IV breast cancer between January 2002 and April 2015 at Methodist Health hospitals and West Cancer Center in Memphis, TN were included in the analysis. Kaplan-Meier survival curves were generated and Cox proportional hazards regression were used to compare survival outcomes among 1342 (38.0%) AA and 2185 (62.0%) non-Hispanic White breast cancer patients by race and breast tumor subtype. Over a mean follow-up time of 29.9 months, AA women displayed increased mortality risk [adjusted hazard ratio (HR), 1.65; 95% confidence interval (CI), 1.35-2.03] and were more likely to be diagnosed at advanced stages of disease. AA women with triple-negative breast cancer (TNBC) had the highest death rate at 26.7% compared to non-Hispanic White women at 16.5%. AA women with TNBC and luminal B/HER2- breast tumors had the highest risk of mortality. Regardless of race, patients who did not have surgery had over five times higher risk of dying compared to those who had surgery. These findings provide additional evidence of the breast cancer disparity gap between AA and non-Hispanic White women and highlight the need for targeted interventions and policies to eliminate breast cancer disparities in AA populations, particularly in Memphis, TN. © 2017 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

EPA Announces the Availability of the Removal Administrative Record for the American Drum and Pallet Site in Memphis, Shelby County, Tennessee, the Daily News - Memphis, TN

EPA Pesticide Factsheets

Contains proof of publication of announcement of availability of the removal administrative record for the American Drum and Pallet Site in Memphis, Shelby County, Tennessee, in the Daily News newspaper Region ID: 04 DocID: 10547927, DocDate: 12-24-2007

Suburban School Opens Elementary Campus in the Heart of Memphis: St. George's Independent School, Memphis, Tennessee

ERIC Educational Resources Information Center

Stewart, Sarah

2016-01-01

St. George's has nearly 1,150 students on three campuses: an elementary campus in Germantown and a middle/upper school campus in Collierville, both suburbs of Memphis, and a second elementary campus in Memphis. The Memphis campus serves 140 students in pre-K-5th grade. All Memphis campus students receive financial aid based on need, and…

75 FR 74002 - Foreign-Trade Zone 148-Knoxville, TN, Application for Subzone Toho, Tenax America, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-30

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 57-2010] Foreign-Trade Zone 148--Knoxville, TN, Application for Subzone Toho, Tenax America, Inc., Extension of Comment Period The comment period for the application for subzone status at the Toho Tenax America, Inc., facility in Rockwood...

76 FR 52733 - Notice of Intent To Rule on Change in Use of Aeronautical Property at Bowling Green-Warren County...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-23

... the FAA Memphis Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN 38118..., Memphis Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN 38118. In addition, a... Administration, Memphis Airports District Office, 2862 Business Park Drive, Building G, Memphis, TN 38118. The...

Regional Vp, Vs, Vp/Vs, and Poisson's ratios across earthquake source zones from Memphis, Tennessee, to St. Louis, Missouri

USGS Publications Warehouse

Catchings, R.D.

1999-01-01

Models of P- and S-wave velocity, Vp/Vs ratios, Poisson's ratios, and density for the crust and upper mantle are presented along a 400-km-long profile trending from Memphis, Tennessee, to St. Louis, Missouri. The profile crosses the New Madrid seismic zone and reveals distinct regional variations in the crustal velocity structure north and south of the latitude of New Madrid. In the south near Memphis, the upper few kilometers of the crust are dominated by upper crustal sedimentary basins or graben with P-wave velocities less than 5 km/sec and S-wave velocities of about 2 km/sec. P-wave velocities of the upper and middle crust range from 6.0 to 6.5 km/sec at depths above 25 km, and corresponding S-wave velocities range from 3.5 to 3.7 km/sec. The lower crust consists of a high-velocity layer (Vp = 7.4 km/sec; Vs ~4.2 km/sec) that is up to 20-km thick at the latitude of New Madrid but thins to about 15 km near Memphis. To the north, beneath the western-most Illinois basin, low-velocity (Vp < 5 km/sec; Vs < 2.3 km/sec) sedimentary basins are less than 1-km deep. The average velocities (Vp = 6.0 km/sec; Vs = 3.5 km/sec) of the underlying, near-surface rocks argue against large thickness of unconsolidated noncarbonate sediments within 50 km of the western edge of the Illinois basin. Most of the crust beneath the Illinois basin is modeled as one layer, with velocities up to 6.8 km/sec (Vs = 3.7 km/sec) at 37-km depth. The thick, high-velocity (Vp = 7.4 km/sec; Vs ~4.2 km/sec) lower crustal layer thins from about 20 km near New Madrid to about 6 km beneath the western Illinois basin. Refractions from the Moho and upper mantle occur as first arrivals over distances as a great as 160 km and reveal upper mantle layering to 60 km depth. Upper mantle layers with P-wave velocities of 8.2 km/sec (Vs = 4.5 km/sec) and 8.4 km/sec (Vs = 4.7 km/sec) are modeled at 43 and 60 km depth, respectively. Crustal Vp/Vs ratios range between 1.74 and 1.83, and upper mantle Vp/V s ratios

Hydrogeology and ground-water flow in the Memphis and Fort Pillow aquifers in the Memphis area, Tennessee

USGS Publications Warehouse

Brahana, J.V.; Broshears, R.E.

2001-01-01

On the basis of known hydrogeology of the Memphis and Fort Pillow aquifers in the Memphis area, a three-layer, finite-difference numerical model was constructed and calibrated as the primary tool to refine understanding of flow in the aquifers. The model was calibrated and tested for accuracy in simulating measured heads for nine periods of transient flow from 1886-1985. Testing and sensitivity analyses indicated that the model accurately simulated observed heads areally as well as through time. The study indicates that the flow system is currently dominated by the distribution of pumping in relation to the distribution of areally variable confining units. Current withdrawal of about 200 million gallons per day has altered the prepumping flow paths, and effectively captured most of the water flowing through the aquifers. Ground-water flow is controlled by the altitude and location of sources of recharge and discharge, and by the hydraulic characteristics of the hydrogeologic units. Leakage between the Fort Pillow aquifer and Memphis aquifer, and between the Memphis aquifer and the water-table aquifers (alluvium and fluvial deposits) is a major component of the hydrologic budget. The study indicates that more than 50 percent of the water withdrawn from the Memphis aquifer in 1980 is derived from vertical leakage across confining units, and the leakage from the shallow aquifer (potential source of contamination) is not uniformly distributed. Simulated leakage was concentrated along the upper reaches of the Wolf and Loosahatchie Rivers, along the upper reaches of Nonconnah Creek, and the surficial aquifer of the Mississippi River alluvial plain. These simulations are supported by the geologic and geophysical evidence suggesting relatively thin or sandy confining units in these general locations. Because water from surficial aquifers is inferior in quality and more susceptible to contamination than water in the deeper aquifers, high rates of leakage to the Memphis

75 FR 6636 - Foreign-Trade Zone 77-Memphis, TN Application for Subzone Cummins, Inc. (Engine Components...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-10

..., gears, pulleys, oil coolers, water pumps, cable, motors, thermostats, electrical components, wiring... merchandise is shipped from the facility and entered for U.S. consumption. Subzone status would further allow Cummins to realize logistical benefits through the use of weekly customs entry procedures. The application...

Geology and ground-water resources of the Memphis Sand in western Tennessee

USGS Publications Warehouse

Parks, William Scott; Carmichael, J.K.

1990-01-01

The Memphis Sand of the Claiborne Group of Tertiary age underlies approximately 7,400 square miles in western Tennessee. The formation primarily consists of a thick body of veryfine to very coarse sand that includes subordinate lenses or beds of clay and silt at various horizons. The Memphis Sand ranges from 0 to about 900 feet in thickness, but where the original thickness is preserved, it is about 400 to 900 feet thick. The Memphis Sand yields water to wells in most of the area of occurrence in western Tennessee and, where saturated, makes up the Memphis aquifer. Recharge to the Memphis aquifer is from precipitation on the outcrop, which is a broad belt across western Tennessee, or by downward infiltration of water from the overlying fluvial deposits of Tertiary(?) and Quatemary age and alluvium of Quatemary age. Long-term data from five observation wells indicate that water levels have declined at average rates rangingfrom less than 0.1 to 1.3 feet per year during the period 1928-83. The largest declines have been in the Memphis area. Water from the Memphis aquifer generally is a calcium bicarbonate type, but locally is a sodium bicarbonate or mixed type. The water contains low concentrations of most major constituents and generally is suitable for most uses. Dissolved-solids concentrations range from 19 to 333 milligrams per liter. The results from 76 aquifer tests made in the Memphis area and western Tennessee during the period 1949-62 indicate that transmissivities range from 2,700 to 53,500 feet squared per day, and storage coefficients range from 0.0001 to 0.003. The Memphis aquifer provides moderate to large quantities of water for many public and industrial water supplies in western Tennessee and small quantities to numerous domestic and farm wells. Withdrawals for public and industrial supplies in 1983 averaged about 227 million gallons per day, of which 183 million gallons per day were in the Memphis area. The Memphis aquifer has much potential for

77 FR 40489 - Amendment of Class E Airspace; Memphis, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

... geographic coordinates of General DeWitt Spain Airport and makes a minor correction to the regulatory text... adjusts the geographic coordinates of General DeWitt Spain Airport to be in concert with the FAAs... Airport (Lat. 34[deg]58'44'' N., long. 89[deg]47'13'' W.) General DeWitt Spain Airport [[Page 40490

Educational Assessment - Memphis.

ERIC Educational Resources Information Center

Memphis Area Chamber of Commerce, TN. Education Committee.

The result of a plan evolved in order to assess the educational potentials of Memphis with a view toward their exploitation to the fullest to meet the manpower demands of the industrial and commercial community today. An analysis was made of present and projected job vacancies and the character of these positions was determined with respect to…

77 FR 51100 - Tennessee Disaster #TN-00068

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-23

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13215 and 13216] Tennessee Disaster TN-00068 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of TENNESSEE dated. 08/16/2012. Incident: Severe storms...

Novel Small Molecule Antagonists of the Interaction of the Androgen Receptor and Transcriptional Co-regulators

DTIC Science & Technology

2009-01-01

ORGANIZATION: St. Jude Research Children’s Hospital Memphis, TN 38105 REPORT DATE...January 2009 TYPE OF REPORT: Annual Summary PREPARED FOR: U.S. Army Medical Research and Materiel Command...PERFORMING ORGANIZATION REPORT NUMBER St. Jude Research Children’s Hospital Memphis, TN

Wake Vortex Field Measurement Program at Memphis, Tennessee: Data Guide

NASA Technical Reports Server (NTRS)

Campbell, S. D.; Dasey, T. J.; Freehart, R. E.; Heinrichs, R. M.; Mathews, M. P.; Perras, G. H.; Rowe, G. S.

1997-01-01

Eliminating or reducing current restrictions in the air traffic control system due to wake vortex considerations would yield increased capacity, decreased delays, and cost savings. Current wake vortex separation standards are widely viewed as very conservative under most conditions. However, scientific uncertainty about wake vortex behavior under different atmospheric conditions remains a barrier to development of an adaptive vortex spacing system. The objective of the wake vortex field measurement efforts during December, 1994 and August, 1995 at Memphis, TN were to record wake vortex behavior for varying atmospheric conditions and types of aircraft. This effort is part of a larger effort by the NASA Langley Research Center to develop an Aircraft Vortex Spacing System (AVOSS) as an element of the Terminal Area Productivity (TAP) program. The TAP program is being performed in concert with the FAA Terminal Air Traffic Control Automation (TATCA) program and ATC Automation. Wake vortex behavior was observed using a mobile continuous-wave (CW) coherent laser Doppler radar (lidar) developed at Lincoln Laboratory. This lidar features a number of improvements over previous systems, including the first-ever demonstration of an automatic wake vortex detection and tracking algorithm.

Gay activism behind the magnolia curtain: the Memphis Gay Coalition, 1979-1991.

PubMed

Buring, D

1996-01-01

In 1979, a full decade after the burgeoning and rapid expansion of the gay liberation movement, a handful of gay men organized the Memphis Gay Coalition and Tennessee's largest city entered the activist mainstream. The Memphis Gay Coalition remained active for over a decade sponsoring political, educational, and social events. After twelve years the Coalition disbanded amid accusations of racism, sexism, and elitism, having achieved some of its original purposes in providing services to the Memphis gay community. Documentary sources and community interviews reveal that personality conflicts, organizational problems, and the forces of Southern culture played a role in the disintegration of the Memphis Gay Coalition, which left the city and its neighboring region without a broad gay activist alliance.

40 CFR 81.44 - Metropolitan Memphis Interstate Air Quality Control Region.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of Air Quality Control Regions § 81.44 Metropolitan Memphis Interstate Air Quality Control Region. The Metropolitan Memphis Interstate Air Quality Control Region (Arkansas-Mississippi-Tennessee) consists of the...

40 CFR 81.44 - Metropolitan Memphis Interstate Air Quality Control Region.

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of Air Quality Control Regions § 81.44 Metropolitan Memphis Interstate Air Quality Control Region. The Metropolitan Memphis Interstate Air Quality Control Region (Arkansas-Mississippi-Tennessee) consists of the...

40 CFR 81.44 - Metropolitan Memphis Interstate Air Quality Control Region.

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of Air Quality Control Regions § 81.44 Metropolitan Memphis Interstate Air Quality Control Region. The Metropolitan Memphis Interstate Air Quality Control Region (Arkansas-Mississippi-Tennessee) consists of the...

40 CFR 81.44 - Metropolitan Memphis Interstate Air Quality Control Region.

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of Air Quality Control Regions § 81.44 Metropolitan Memphis Interstate Air Quality Control Region. The Metropolitan Memphis Interstate Air Quality Control Region (Arkansas-Mississippi-Tennessee) consists of the...

40 CFR 81.44 - Metropolitan Memphis Interstate Air Quality Control Region.

Code of Federal Regulations, 2011 CFR

2011-07-01

... (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF AREAS FOR AIR QUALITY PLANNING PURPOSES Designation of Air Quality Control Regions § 81.44 Metropolitan Memphis Interstate Air Quality Control Region. The Metropolitan Memphis Interstate Air Quality Control Region (Arkansas-Mississippi-Tennessee) consists of the...

77 FR 17360 - Proposed Amendment of Class E Airspace; Memphis, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-26

... invited on the overall regulatory, aeronautical, economic, environmental, and energy-related aspects of..., when promulgated, would not have a significant economic impact on a substantial number of small... Branch, MS, Olive Branch Airport (Lat. 34[deg]58'44'' N., long. 89[deg]47'13'' W.) General DeWitt Spain...

Novel Small Molecule Antagonists of the Interaction of the Androgen Receptor and Transcriptional Co-regulators

DTIC Science & Technology

2008-01-31

CONTRACTING ORGANIZATION: St. Jude Research Children’s Hospital Memphis, TN 38105 REPORT DATE: January 2008 TYPE OF REPORT: Annual...Summary PREPARED FOR: U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012... Research Children’s Hospital Memphis, TN 38105 9. SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S

Molecular dynamics studies on troponin (TnI-TnT-TnC) complexes: insight into the regulation of muscle contraction.

PubMed

Varughese, Jayson F; Chalovich, Joseph M; Li, Yumin

2010-10-01

Mutations of any subunit of the troponin complex may lead to serious disorders. Rational approaches to managing these disorders require knowledge of the complex interactions among the three subunits that are required for proper function. Molecular dynamics (MD) simulations were performed for both skeletal (sTn) and cardiac (cTn) troponin. The interactions and correlated motions among the three components of the troponin complex were analyzed using both Molecular Mechanics-Generalized Born Surface Area (MMGBSA) and cross-correlation techniques. The TnTH2 helix was strongly positively correlated with the two long helices of TnI. The C domain of TnC was positively correlated with TnI and TnT. The N domain of TnC was negatively correlated with TnI and TnT in cTn, but not in sTn. The two C-domain calcium-binding sites of TnC were dynamically correlated. The two regulatory N-domain calcium-binding sites of TnC were dynamically correlated, even though the calcium-binding site I is dysfunctional. The strong interaction residue pairs and the strong dynamically correlated residues pairs among the three components of troponin complexes were identified. These correlated motions are consistent with the idea that there is a high degree of cooperativity among the components of the regulatory complex in response to Ca(2+) and other effectors. This approach may give insight into the mechanism by which mutations of troponin cause disease. It is interesting that some observed disease causing mutations fall within regions of troponin that are strongly correlated or interacted.

77 FR 26667 - Standard Instrument Approach Procedures, and Takeoff Minimums and Obstacle Departure Procedures...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-07

.... 97.20 of Title 14 of the Code of Federal Regulations. The large number of SIAPs, their complex nature...-12 MN Mankato Mankato Rgnl........ 2/1869 4/20/12 RNAV (GPS) RWY 22, Orig 31-May-12 TN Memphis... IL Chicago Chicago O'Hare Intl. 2/6031 4/11/12 ILS OR LOC RWY 4R, Amdt 6L 31-May-12 MN Cloquet...

TREATMENT OF GROUND WATER WITH ZERO VALENT IRON (ZVI)

EPA Science Inventory

A presentation on the use of zero valent iron (ZVI) for groundwater remediation at the Memphis Defense Depot Site in Memphis, TN, will be given at a public meeting in Memphis on February 24. The presentation is being given in response to a request by a citizen's group associated...

9 CFR 77.28 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... TUBERCULOSIS Captive Cervids § 77.28 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.11 - Modified accredited States or zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... TUBERCULOSIS Cattle and Bison § 77.11 Modified accredited States or zones. (a) The following are modified... Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is incorporated by reference... accredited States or zones and its being reclassified as accreditation preparatory. (d) If tuberculosis is...

9 CFR 77.11 - Modified accredited States or zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... TUBERCULOSIS Cattle and Bison § 77.11 Modified accredited States or zones. (a) The following are modified... Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is incorporated by reference... accredited States or zones and its being reclassified as accreditation preparatory. (d) If tuberculosis is...

9 CFR 77.28 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... TUBERCULOSIS Captive Cervids § 77.28 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.11 - Modified accredited States or zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... TUBERCULOSIS Cattle and Bison § 77.11 Modified accredited States or zones. (a) The following are modified... Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is incorporated by reference... accredited States or zones and its being reclassified as accreditation preparatory. (d) If tuberculosis is...

9 CFR 77.28 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... TUBERCULOSIS Captive Cervids § 77.28 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.28 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... TUBERCULOSIS Captive Cervids § 77.28 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.28 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... TUBERCULOSIS Captive Cervids § 77.28 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

77 FR 13195 - Amendment of Class E Airspace; Springfield, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-06

..., Georgia 30320; telephone (404) 305-6364. SUPPLEMENTARY INFORMATION: History On September 22, 2011, the FAA... areas extending upward from 700 feet or more above the surface of the earth. * * * * * ASO TN E5...

9 CFR 77.13 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... TUBERCULOSIS Cattle and Bison § 77.13 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.13 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... TUBERCULOSIS Cattle and Bison § 77.13 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.13 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... TUBERCULOSIS Cattle and Bison § 77.13 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.13 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... TUBERCULOSIS Cattle and Bison § 77.13 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.13 - Accreditation preparatory States or zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... TUBERCULOSIS Cattle and Bison § 77.13 Accreditation preparatory States or zones. (a) The following are... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition), which is... tuberculosis is diagnosed within an accreditation preparatory State or zone in an animal not specifically...

9 CFR 77.11 - Modified accredited States or zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... TUBERCULOSIS Cattle and Bison § 77.11 Modified accredited States or zones. (a) The following are modified... the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition), which is...) If tuberculosis is diagnosed within a modified accredited State or zone in an animal not specifically...

Estimation of earthquake effects associated with a great earthquake in the New Madrid seismic zone

USGS Publications Warehouse

Hopper, Margaret G.; Algermissen, Sylvester Theodore; Dobrovolny, Ernest E.

1983-01-01

Estimates have been made of the effects of a large Ms = 8.6, Io = XI earthquake hypothesed to occur anywhere in the New Madrid seismic zone. The estimates are based on the distributions of intensities associated with the earthquakes of 1811-12, 1843 and 1895 although the effects of other historical shocks are also considered. The resulting composite type intensity map for a maximum intensity XI is believed to represent the upper level of shaking likely to occur. Specific intensity maps have been developed for six cities near the epicentral region taking into account the most likely distribution of site response in each city. Intensities found are: IX for Carbondale, IL; VIII and IX for Evansville, IN; VI and VIII for Little Rock, AR; IX and X for Memphis, TN; VIII, IX, and X for Paducah, KY; and VIII and X for Poplar Bluff, MO. On a regional scale, intensities are found to attenuate from the New Madrid seismic zone most rapidly to the west and southwest sides of the zone, most slowly to the northwest along the Mississippi River, on the northeast along the Ohio River, and on the southeast toward Georgia and South Carolina. Intensities attenuate toward the north, east, and south in a more normal fashion. Known liquefaction effects are documented but much more research is needed to define the liquefaction potential.

9 CFR 77.26 - Modified accredited States or zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... TUBERCULOSIS Captive Cervids § 77.26 Modified accredited States or zones. (a) States listed in paragraph (b) of... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition... preparatory. (e) If tuberculosis is diagnosed within a modified accredited State or zone in an animal not...

9 CFR 77.26 - Modified accredited States or zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... TUBERCULOSIS Captive Cervids § 77.26 Modified accredited States or zones. (a) States listed in paragraph (b) of... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition... preparatory. (e) If tuberculosis is diagnosed within a modified accredited State or zone in an animal not...

9 CFR 77.26 - Modified accredited States or zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... TUBERCULOSIS Captive Cervids § 77.26 Modified accredited States or zones. (a) States listed in paragraph (b) of... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition... preparatory. (e) If tuberculosis is diagnosed within a modified accredited State or zone in an animal not...

9 CFR 77.26 - Modified accredited States or zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... TUBERCULOSIS Captive Cervids § 77.26 Modified accredited States or zones. (a) States listed in paragraph (b) of... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition... preparatory. (e) If tuberculosis is diagnosed within a modified accredited State or zone in an animal not...

9 CFR 77.26 - Modified accredited States or zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... TUBERCULOSIS Captive Cervids § 77.26 Modified accredited States or zones. (a) States listed in paragraph (b) of... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999, edition... preparatory. (e) If tuberculosis is diagnosed within a modified accredited State or zone in an animal not...

Memphis District Reels from Operations Woes

ERIC Educational Resources Information Center

Gewertz, Catherine

2008-01-01

The Memphis, Tennessee, school district is trying to steady itself in the wake of controversies that have rocked its operations side, potentially complicating its search for a new superintendent and renewing talk about putting the city's mayor in charge of the system. In the past few months, the district has been contending with official probes of…

Compound 49b Reduces Inflammatory Markers and Apoptosis after Ocular Blast Injury

DTIC Science & Technology

2018-02-01

Vanessa M. Morales-Tirado MS, PhD CONTRACTING ORGANIZATION: University of Tennessee Health Science Center Memphis, TN 38163 REPORT DATE: February...which are critical for the visual output from the eye to the brain in health and ocular injury. 15. SUBJECT TERMS 16. SECURITY CLASSIFICATION OF: 17...February 2018 15Aug2012 - 14Aug2015 Dr. Vanessa Morales vmorale1@uthsc.edu University of Tennessee Health Science Center Memphis, TN 38163 W81XWH

Mitigating Security Issues: The University of Memphis Case.

ERIC Educational Resources Information Center

Jackson, Robert; Frolick, Mark N.

2003-01-01

Studied a server security breach at the University of Memphis, Tennessee, to highlight personnel roles, detection of the compromised server, policy enforcement, forensics, and the proactive search for other servers threatened in the same way. (SLD)

9 CFR 77.4 - Application for and retention of zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Application for and retention of zones. 77.4 Section 77.4 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS...

9 CFR 77.4 - Application for and retention of zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Application for and retention of zones. 77.4 Section 77.4 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS...

9 CFR 77.4 - Application for and retention of zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Application for and retention of zones. 77.4 Section 77.4 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS...

9 CFR 77.4 - Application for and retention of zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Application for and retention of zones. 77.4 Section 77.4 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS...

9 CFR 77.4 - Application for and retention of zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Application for and retention of zones. 77.4 Section 77.4 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS...

Hydrogeology, ground-water quality, and source of ground water causing water-quality changes in the Davis well field at Memphis, Tennessee

USGS Publications Warehouse

Parks, William S.; Mirecki, June E.; Kingsbury, James A.

1995-01-01

NETPATH geochemical model code was used to mix waters from the alluvial aquifer with water from the Memphis aquifer using chloride as a conservative tracer. The resulting models indicated that a mixture containing 3 percent alluvial aquifer water mixed with 97 percent unaffected Memphis aquifer water would produce the chloride concentration measured in water from the Memphis aquifer well most affected by water-quality changes. NETPATH also was used to calculate mixing percentages of alluvial and Memphis aquifer Abstract waters based on changes in the concentrations of selected dissolved major inorganic and trace element constituents that define the dominant reactions that occur during mixing. These models indicated that a mixture containing 18 percent alluvial aquifer water and 82 percent unaffected Memphis aquifer water would produce the major constituent and trace element concentrations measured in water from the Memphis aquifer well most affected by water-quality changes. However, these model simulations predicted higher dissolved methane concentrations than were measured in water samples from the Memphis aquifer wells.

9 CFR 77.24 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... TUBERCULOSIS Captive Cervids § 77.24 Modified accredited advanced States or zones. (a) The following are... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition... modified accredited. (d) If tuberculosis is diagnosed within a modified accredited advanced State or zone...

9 CFR 77.24 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... TUBERCULOSIS Captive Cervids § 77.24 Modified accredited advanced States or zones. (a) The following are... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition... modified accredited. (d) If tuberculosis is diagnosed within a modified accredited advanced State or zone...

9 CFR 77.24 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... TUBERCULOSIS Captive Cervids § 77.24 Modified accredited advanced States or zones. (a) The following are... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition... modified accredited. (d) If tuberculosis is diagnosed within a modified accredited advanced State or zone...

9 CFR 77.24 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... TUBERCULOSIS Captive Cervids § 77.24 Modified accredited advanced States or zones. (a) The following are... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition... modified accredited. (d) If tuberculosis is diagnosed within a modified accredited advanced State or zone...

9 CFR 77.24 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... TUBERCULOSIS Captive Cervids § 77.24 Modified accredited advanced States or zones. (a) The following are... contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999 edition... modified accredited. (d) If tuberculosis is diagnosed within a modified accredited advanced State or zone...

9 CFR 77.9 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... TUBERCULOSIS Cattle and Bison § 77.9 Modified accredited advanced States or zones. (a) The following are... requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999... modified accredited. (d) If tuberculosis is diagnosed within a modified accredited advanced State or zone...

9 CFR 77.3 - Tuberculosis classifications of States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Tuberculosis classifications of States... TUBERCULOSIS General Provisions § 77.3 Tuberculosis classifications of States and zones. The Administrator shall classify each State for tuberculosis in accordance with this part. A zone comprising less than an...

9 CFR 77.9 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... TUBERCULOSIS Cattle and Bison § 77.9 Modified accredited advanced States or zones. (a) The following are... “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999), which is incorporated by... accredited advanced States or zones and its being reclassified as modified accredited. (d) If tuberculosis is...

9 CFR 77.3 - Tuberculosis classifications of States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Tuberculosis classifications of States... TUBERCULOSIS General Provisions § 77.3 Tuberculosis classifications of States and zones. The Administrator shall classify each State for tuberculosis in accordance with this part. A zone comprising less than an...

9 CFR 77.3 - Tuberculosis classifications of States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Tuberculosis classifications of States... TUBERCULOSIS General Provisions § 77.3 Tuberculosis classifications of States and zones. The Administrator shall classify each State for tuberculosis in accordance with this part. A zone comprising less than an...

9 CFR 77.3 - Tuberculosis classifications of States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Tuberculosis classifications of States... TUBERCULOSIS General Provisions § 77.3 Tuberculosis classifications of States and zones. The Administrator shall classify each State for tuberculosis in accordance with this part. A zone comprising less than an...

9 CFR 77.3 - Tuberculosis classifications of States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Tuberculosis classifications of States... TUBERCULOSIS General Provisions § 77.3 Tuberculosis classifications of States and zones. The Administrator shall classify each State for tuberculosis in accordance with this part. A zone comprising less than an...

Genetic and molecular studies of a composite chromosomal element (Tn3705) containing a Tn916-modified structure (Tn3704) in Streptococcus anginosus F22.

PubMed

Clermont, D; Horaud, T

1994-01-01

The plasmid-free Streptococcus anginosus F22 contained a conjugative element, Tn3705, encoding resistance to erythromycin (Emr) and tetracycline-minocycline (Tcr-Mnr). We mapped a chromosomal region (> 52 kb) of F22, corresponding to the internal part of Tn3705. Molecular analysis of Tn3705 revealed it to be a composite structure: it included in its central part a transposon designated Tn3704 (20.3 kb +/- 0.5 kb), which had a modified structure in comparison with that of Tn916 and on which the Emr Tcr-Mn4 markers were localized. Tn3705 inserted from F22 into the chromosome of various streptococcal transconjugants as well as that of Enterococcus faecalis transconjugants without changing its structure. In contrast, from the chromosome of an E. faecalis::Tn3705 transconjugant only Tn3704 inserted, at various sites, into another E. faecalis chromosome. Sugar fermentations occurred after the insertion of Tn3704 into the chromosome of an asaccharolytic E. faecalis strain. Transposition of only Tn3704 from the chromosome of E. faecalis::Tn3705 onto pIP964, an E. faecalis hemolysin plasmid, yielded two different pIP964 derivatives. The size of the entire element Tn3705 was estimated to be about 70.0 kb by pulsed-field electrophoresis.

Chemical character of ground water in the shallow water-table aquifer at selected localities in the Memphis area, Tennessee

USGS Publications Warehouse

Parks, William Scott; Graham, D.D.; Lowery, J.F.

1981-01-01

Eight deep wells are being monitored in the Memphis, Tenn., area to detect any changes in the chemical character of water moving through the Memphis Sand towards major pumping centers. These wells are strategically located so as to intercept groundwater enroute through the Memphis Sand from the outcrop-recharge area. Although water quality analyses are available for many wells in the shallow water-table aquifer, no specific investigation has been made to characterize the quality of the water in this aquifer from which the Memphis Sand also receives part of its recharge. This investigation is to determine the chemical character of groundwater in the shallow water-table aquifer at selected localities in the Memphis area. Methods used to install eight shallow wells at abandoned dump sites containing chemical and/or industrial waste are described. Water samples from the eight shallow wells and two deep wells in the Memphis Sand were collected and analyzed. Results of the analysis are presented and the locations of the wells and dumps are shown on maps. (USGS)

A preliminary report on the artesian water supply of Memphis, Tennessee

USGS Publications Warehouse

Wells, F.G.

1932-01-01

Memphis is located in the part of the Gulf Coastal Plain known as the Mississippi embayment. It is underlain by unconsolidated sand and clay formations of Tertiary and Cretaceous age. The Wilcox group, of Tertiary age, and the Ripley formation, of Cretaceous age, are excellent aquifers, and all the water consumed in Memphis is derived from them. The maximum pumpage from the Wilcox group was reached about 1920; in that year an estimated average of 37,575,000 gallons a day was pumped. In 1928 the average daily pumpage from the Wilcox group was about 33,984,000 gallons, and in addition to this the Memphis Artesian Water Department pumped an average of 4,616,000 gallons a day from the Ripley formation. The static level at Memphis varies with the pumpage and the stage of the Mississippi River. The original static level was about 235 feet above mean sea level. In 1928 the average static level at the Auction Avenue plant was 202 feet above mean sea level, which was about 33 feet lower than the original level. The yield is therefore about a million gallons a day for each foot of drawdown. The drawdown is not excessive, and additional pumpage can be developed without undue lowering of head. The water from both the Wilcox group and the Ripley formation is fairly soft and has a moderately low content of dissolved mineral matter. The iron content is sufficiently high to be objectionable, but the iron is easily removed by aeration followed by either settling or filtration for removal of sediment.

Feasibility study of transportation management strategies in the Poplar Corridor, Memphis, Tennessee

DOE Office of Scientific and Technical Information (OSTI.GOV)

Siniard, D.

1990-02-01

This report documents the development and implementation of various transportation management strategies aimed at alleviating traffic congestion problems in the Poplar Corridor, a major transportation corridor located in a rapidly growing suburban area of Memphis, Tennessee. The project provided the opportunity for local governments to work with the private sector in a joint venture to address traffic congestion problems and to promote more efficient use of the area's transportation network. The project was carried out by the staff of Memphis Area Rideshare, a joint city/county agency which provides transit information and free carpool/vanpool computer matching services to area commuters. Publicmore » sector participants in the planning process included transportation and land use planners from the Office of Planning and Development, city traffic engineers, and representatives from the Memphis Area Transit Authority (MATA). Private sector input came from major developers and employers in the Poplar Corridor and from officials of schools located in the area.« less

Predicted hydrologic effects of pumping from the Lichterman Well Field in the Memphis Area, Tennessee

USGS Publications Warehouse

Nyman, Dale J.

1965-01-01

The Lichterman well field is scheduled to go into operation early in 1965 to supplement the municipal water-supply system for the city of Memphis, Tenn. Although the initial rate of withdrawal from the well field will be about 8 mgd (million gallons per day), the ultimate design capacity of the field is 20 mgd. A study of sand samples, drillers' logs, and geophysical logs collected during preliminary test drilling at the site for the Lichterman well field was used as a basis for defining three zones of sand favorable for the construction of high-capacity (1,000 gallons per minute or more) water wells. The three zones occur in the '500-foot' sand and are here designated (in descending order) as zone A, zone B, and zone C. The depth to the top of these zones below land surface has the following ranges: zone A, 125 to 225 feet; zone B, 200 to 350 feet; and zone C, 700 to 775 feet. Zones A and B range from 0 to 100 feet in thickness, and zone C ranges from 10 to 100 feet in thickness. Within the well field proper these zones are expected to react to the stress of pumping as separate hydrologic units, but outside the well field the three zones are expected to react as a single hydrologic unit. The '500-foot' sand in the Germantown-Collierville area is recharged chiefly by precipitation on the outcrop area of the sand to the east, but the evidence indicates that additional recharge is entering the aquifer from the Wolf River. In spite of this additional recharge, water levels in the '500-foot' sand are declining at an average rate of about two-thirds of a foot per year, owing to municipal and industrial pumpage in the Memphis area. However, this decline is not expected to alter the excellent quality of the water in the '500-foot' sand at the site of the Lichterman well field. Pumping in the Lichterman well field will create a cone of depression in the free-water (piezometric) surface of the '500-foot' sand. The decline in water levels will be directly proportional to the

9 CFR 77.8 - Interstate movement from accredited-free States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.8 Interstate movement from accredited-free States and zones. Cattle or bison that originate in an accredited-free State or zone may be moved interstate without...

78 FR 76171 - Endangered Species; Receipt of Applications for Permit

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-16

... notification covers activities to be conducted by the applicant over a 5-year period. Applicant: Theresa.... This notification covers activities to be conducted by the applicant over a 5-year period. Applicant...-year period. Applicant: Memphis Zoological Garden, Memphis, TN; PRT-671021 The applicant requests...

Mississippi River, Yazoo Basin, Memphis, TN

NASA Image and Video Library

1973-06-22

SL2-05-422 (22 June 1973) --- This section of the lower Mississippi River (34.0N, 90.0W) known as the Yazoo Basin, is characterized by a wide expanse of rich river bottomland with many oxbow lakes, the remains of the many changes in the riverbed over the course of many thousands of years. This soil is very fertile and productive but the region is prone to flooding. In this view, some of the back areas around the Delta National Forest show the effects of heavy spring rains. Photo credit: NASA

Central US earthquake catalog for hazard maps of Memphis, Tennessee

USGS Publications Warehouse

Wheeler, R.L.; Mueller, C.S.

2001-01-01

An updated version of the catalog that was used for the current national probabilistic seismic-hazard maps would suffice for production of large-scale hazard maps of the Memphis urban area. Deaggregation maps provide guidance as to the area that a catalog for calculating Memphis hazard should cover. For the future, the Nuttli and local network catalogs could be examined for earthquakes not presently included in the catalog. Additional work on aftershock removal might reduce hazard uncertainty. Graphs of decadal and annual earthquake rates suggest completeness at and above magnitude 3 for the last three or four decades. Any additional work on completeness should consider the effects of rapid, local population changes during the Nation's westward expansion. ?? 2001 Elsevier Science B.V. All rights reserved.

Memphis Belle and Crew Visit the Aircraft Engine Research Laboratory

NASA Image and Video Library

1943-07-21

Captain Robert Morgan and the rest of the Memphis Belle crew arrive in Cleveland on a rainy July 7, 1943, for three-day publicity visit. This B–17 Flying Fortress had recently become the first U.S. bomber to complete 25 missions over Germany and France. The lack of long distance escort fighters made the feat even more remarkable. The Memphis Belle and its crew returned to the United States in June and were immediately thrown into a three-month-long war bond tour. While in Cleveland the crew toured the National Advisory Committee for Aeronautics (NACA) Aircraft Engine Research Laboratory, the Cleveland Bomber Plant, and Thompson Products. In the evenings they were feted downtown by the Chamber of Commerce at the Hotel Cleveland. A local company brought Morgan’s family and his fiancé—the Memphis Belle’s inspiration—to Cleveland to participate in the activities. The bomber was on display to the public near the airport’s fenceline and stored in the NACA’s hangar overnight. Pictured in this photograph from left to right: Robert Hanson, Vincent Evans, Charles Leighton, NACA Manager Raymond Sharp, Robert Morgan, William Holliday of the Chamber of Commerce, Army Liaison Officer Colonel Edwin Page, Airport Commissioner Jack Berry, Cecil Scott, John Quinlan and James Verinis. Kneeling are Harold Loch, Casimer Nastal and Charles Wichell.

Increasing Retention in STEM: Results from a STEM Talent Expansion Program at the University of Memphis

ERIC Educational Resources Information Center

Windsor, Alistair; Bargagliotti, Anna; Best, Rachel; Franceschetti, Donald; Haddock, John; Ivey, Stephanie; Russomanno, David

2015-01-01

MemphiSTEP is a five-year STEM Talent Expansion Program (STEP) at the University of Memphis sponsored by the National Science Foundation. The project focuses on retention and persistence to graduation to increase the number of STEM majors and graduates. The project includes a range of student retention programs, including a Summer Mathematics…

Altitude of the top of the Sparta Sand and Memphis Sand in three areas of Arkansas

USGS Publications Warehouse

Pugh, Aaron L.; Westerfield, Paul W.; Gonthier, Gerard; Poynter, David T.

1998-01-01

The Sparta Sand and Memphis Sand form the second most productive aquifer in Arkansas. The Sparta Sand and Memphis Sand range in thick- ness from 0 to 900 feet, consisting of fine- to medium-grained sands interbedded with layers of silt, clay, shale, and minor amounts of lignite. Within the three areas of interest, the top surface of the Sparta Sand and Memphis Sand dips regionally east and southeast towards the axis of the Mississippi Embayment syncline and Desha Basin. Local variations in the top surface may be attributed to a combination of continued development of structural features, differential compaction, localized faulting, and erosion of the surface prior to subsequent inundation and deposition of younger sediments.

9 CFR 77.29 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Interstate movement from accreditation preparatory States and zones. 77.29 Section 77.29 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.12 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Interstate movement from modified accredited States and zones. 77.12 Section 77.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.14 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Interstate movement from accreditation preparatory States and zones. 77.14 Section 77.14 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.29 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Interstate movement from accreditation preparatory States and zones. 77.29 Section 77.29 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.14 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Interstate movement from accreditation preparatory States and zones. 77.14 Section 77.14 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.12 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Interstate movement from modified accredited States and zones. 77.12 Section 77.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.14 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Interstate movement from accreditation preparatory States and zones. 77.14 Section 77.14 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.29 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Interstate movement from accreditation preparatory States and zones. 77.29 Section 77.29 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.27 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Interstate movement from modified accredited States and zones. 77.27 Section 77.27 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.29 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate movement from accreditation preparatory States and zones. 77.29 Section 77.29 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.12 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Interstate movement from modified accredited States and zones. 77.12 Section 77.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.27 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Interstate movement from modified accredited States and zones. 77.27 Section 77.27 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.14 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate movement from accreditation preparatory States and zones. 77.14 Section 77.14 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.27 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Interstate movement from modified accredited States and zones. 77.27 Section 77.27 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.12 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Interstate movement from modified accredited States and zones. 77.12 Section 77.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.29 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Interstate movement from accreditation preparatory States and zones. 77.29 Section 77.29 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.12 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate movement from modified accredited States and zones. 77.12 Section 77.12 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.27 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate movement from modified accredited States and zones. 77.27 Section 77.27 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.27 - Interstate movement from modified accredited States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Interstate movement from modified accredited States and zones. 77.27 Section 77.27 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.14 - Interstate movement from accreditation preparatory States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Interstate movement from accreditation preparatory States and zones. 77.14 Section 77.14 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

EMCS Modules/Intelligent Time Clock (ITC).

DTIC Science & Technology

1980-09-01

scale, smaller activities where a large EMCS would not be economically feasible, have had to look elsewhere to achieve their conservation goals. In this...DAEN- MPU . Washington DC: ERADCOM Tech Supp Dir. (DELSD-L) Ft. Monmouth, NJ: Engr District (Memphis) Library. Memphis TN: Natick (’en (Kwvoh flu

9 CFR 77.11 - Modified accredited States or zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... TUBERCULOSIS Cattle and Bison § 77.11 Modified accredited States or zones. (a) The following are modified... herd test requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication... reclassified as accreditation preparatory. (d) If tuberculosis is diagnosed within a modified accredited State...

[Construction of cTnC-linker-TnI (P) Genes, Expression of Fusion Protein and Preparation of Lyophilized Protein].

PubMed

Song, Xiaoli; Liu, Xiaoyun; Cai, Lei; Wu, Jianwei; Wang, Jihua

2015-12-01

In order to construct and express human cardiac troponin C-linker-troponin I(P) [ cTnC-linker-TnI(P)] fusion protein, detect its activity and prepare lyophilized protein, we searched the CDs of human cTnC and cTnI from GenBank, synthesized cTnC and cTnI(30-110aa) into cloning vector by a short DNA sequence coding for 15 neutral amino acid residues. pCold I-cTnC-linker-TnI(P) was constructed and transformed into E. coli BL21(DE3). Then, cTnC-linker-TnI(P) fusion protein was induced by isopropyl-β-D-thiogalactopyranoside (IPTG). Soluable expression of cTnC-linker-TnI(P) in prokaryotic system was successfully obtained. The fusion protein was purified by Ni²⁺ Sepharose 6 Fast Flow affinity chromatography with over 95% purity and prepared into lyophilized protein. The activity of purified cTnC-linker-TnI(P) and its lyophilized protein were detected by Wondfo Finecare™ cTnI Test. Lyophilized protein of cTnC-linker-TnI(P) was stable for 10 or more days at 37 °C and 4 or more months at 25 °C and 4 °C. The expression system established in this research is feasible and efficient. Lyophilized protein is stable enough to be provided as biological raw materials for further research.

An Abstract of the Report on the Public School System of Memphis, Tennessee. Bulletin, 1919, No. 72

ERIC Educational Resources Information Center

Bureau of Education, Department of the Interior, 1919

1919-01-01

During May and June, 1919, the United States Bureau of Education, under the direction of the Commissioner of Education, made a survey of the public-school system of Memphis, Tennessee. This survey included a study of the social and industrial conditions of Memphis, recommendations in regard to the organization, supervision, and financing of the…

Hydrogeology, ground-water quality, and potential for water-supply contamination near the Shelby County landfill in Memphis, Tennessee

USGS Publications Warehouse

Parks, W.S.; Mirecki, J.E.

1992-01-01

An investigation was conducted from 1989 to 1991 to collect and interpret hydrogeologic and ground-water-quality data specific to the Shelby County landfill in east Memphis, Tennessee. Eighteen wells were installed in the alluvial and Memphis aquifers at the landfill. Hydrogeologic data collected showed that the confining unit separating the alluvial aquifer from the Memphis aquifer was thin or absent just north of the landfill and elsewhere consists predominantly of fine sand and silt with lenses of clay. A water-table map of the landfill vicinity confirms the existence of a depression in the water table north and northeast of the landfill and indicates that ground water flows northeast from the Wolf River passing beneath the landfill toward the depression in the water table. A map of the potentiometric surface of the Memphis aquifer shows that water levels were anomalously high just north of the landfill, indicating downward leakage of water from the alluvial aquifer to the Memphis aquifer. An analysis of water-quality data for major and trace inorganic constituents and nutrients confirms that leachate from the landfill has migrated northeastward in the alluvial aquifer toward the depression in the water table and that contaminants in the alluvial aquifer have migrated downward into the Memphis aquifer. The leachate plume can be characterized by concentrations of certain major and trace inorganic constituents that are 2 to 20 times higher than samples from upgradient and background alluvial aquifer wells. The major and trace constituents that best characterize the leachate plume are total organic carbon, chloride, dissolved solids, iron, ammonia nitrogen, calcium, sodium, iodide, barium, strontium, boron, and cadmium. Several of these constituents (specifically dissolved solids, calcium, sodium, and possibly ammonia nitrogen, chloride, barium, and strontium) were detected in elevated concentrations in samples from certain Memphis aquifer wells. Elevated

Potentiometric Surface in the Sparta-Memphis Aquifer of the Mississippi Embayment, Spring 2007

USGS Publications Warehouse

Schrader, T.P.

2008-01-01

The most widely used aquifer for industry and public supply in the Mississippi embayment in Arkansas, Louisiana, Mississippi, and Tennessee is the Sparta-Memphis aquifer. Decades of pumping from the Sparta-Memphis aquifer have affected ground-water levels throughout the Mississippi embayment. Regional assessments of water-level data from the aquifer are important to document regional water-level conditions and to develop a broad view of the effects of ground-water development and management on the sustainability and availability of the region's water supply. This information is useful to identify areas of water-level declines, identify cumulative areal declines that may cross State boundaries, evaluate the effectiveness of ground-water management strategies practiced in different States, and identify areas with substantial data gaps that may preclude effective management of ground-water resources. A ground-water flow model of the northern Mississippi embayment is being developed by the Mississippi Embayment Regional Aquifer Study (MERAS) to aid in answering questions about ground-water availability and sustainability. The MERAS study area covers parts of eight states including Alabama, Arkansas, Illinois, Kentucky, Louisiana, Mississippi, Missouri, and Tennessee and covers approximately 70,000 square miles. The U.S. Geological Survey (USGS) and the Mississippi Department of Environmental Quality Office of Land and Water Resources measured water levels in wells completed in the Sparta-Memphis aquifer in the spring of 2007 to assist in the MERAS model calibration and to document regional water-level conditions. Measurements by the USGS and the Mississippi Department of Environmental Quality Office of Land and Water Resources were done in cooperation with the Arkansas Natural Resources Commission; the Arkansas Geological Survey; Memphis Light, Gas and Water; Shelby County, Tennessee; and the city of Germantown, Tennessee. In 2005, total water use from the Sparta-Memphis

Flow pattern and related chemical quality of ground water in the "500-foot" sand in the Memphis area, Tennessee

USGS Publications Warehouse

Bell, Edwin Allen; Nyman, Dale J.

1968-01-01

The '500-foot' sand is the major source of water supply for the Memphis area. Thick layers of impervious clay above and below the sand confine the water in the aquifer under artesian pressure and also protect the aquifer from contamination. Recharge from rainfall enters the '500-foot' sand in the outcrop, or intake area south and east of Memphis. Recharge from other aquifers enters the sand wherever the confining beds are breached or absent. Some of the recharge that enters the '500-foot' sand in eastern Arkansas moves down the gradients created by pumping in the Memphis area. All discharge from the '500-foot' sand in the Memphis area results from well pumping. Since 1886 continuous withdrawals at gradually increasing rates of pumping have lowered water levels and altered hydraulic gradients in the area. These withdrawals have resulted in changes in direction and velocity of movement of water through the '500-foot' sand. Water in the sand in the southeaster n part of the Memphis area normally moves from the (outcrop area east and south of Memphis northwestward toward points of withdrawal. In the northwestern part of the area, water moves southeastward toward points of withdrawal. A flow-net analysis of the aquifer shows that the rate of water movement through the '500-foot' sand in 1964, toward the major cones of depression in the Memphis area, was about 350 feet per year, or 1 mile in 15 years. A flow-net analysis projected for the year 1975 indicates the rate will increase by about 20 percent in the 12-year period 1964-75. Water in the '500-foot' sand in the Memphis area is generally a calcium magnesium sodium bicarbonate type. It is soft, low in dissolved solids, high in concentrations of iron and carbon dioxide, and slightly to moderately corrosive. The softest and least mineralized water occurs in the southeastern part of the area, and the water becomes slightly harder and more mineralized as it moves downdip toward Memphis. The hardest and most mineralized

9 CFR 77.10 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Interstate movement from modified accredited advanced States and zones. 77.10 Section 77.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.10 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Interstate movement from modified accredited advanced States and zones. 77.10 Section 77.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.10 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Interstate movement from modified accredited advanced States and zones. 77.10 Section 77.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.10 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Interstate movement from modified accredited advanced States and zones. 77.10 Section 77.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

9 CFR 77.10 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Interstate movement from modified accredited advanced States and zones. 77.10 Section 77.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND...

FHWA mid-south megaregion workshop : Memphis, Tennessee; December 8th, 2016

DOT National Transportation Integrated Search

2016-12-08

This report reviews and highlights key content and outcomes identified at the Mid-South Megaregion Workshop held on December 8, 2016 in Memphis Tennessee. Sponsored by the Federal Highway Administrations (FHWA) Office of Planning, Environment, & R...

In-Depth Portfolio Assessment: Shelby County Schools, Memphis, Tennessee

ERIC Educational Resources Information Center

Campbell, Christine; Binder, Libuse

2014-01-01

The 2013 merger of Memphis City Schools (with 103,000 students) and Shelby County Schools (with 47,000 students) was the largest school district consolidation in American history. In its first year of operation, the new Shelby County Schools (SCS) commissioned CRPE researchers to perform a critical review of the district's readiness to implement a…

9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

9 CFR 77.16 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... PRODUCTS TUBERCULOSIS Cattle and Bison § 77.16 Interstate movement from nonaccredited States and zones... with or exposed to tuberculosis, may be moved interstate only if the cattle or bison are accompanied by...

9 CFR 77.31 - Interstate movement from nonaccredited States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... PRODUCTS TUBERCULOSIS Captive Cervids § 77.31 Interstate movement from nonaccredited States and zones... or exposed to tuberculosis may not be moved interstate only if they are accompanied by VS Form 1-27...

Challenges in Antibody Development against Tn and Sialyl-Tn Antigens

PubMed Central

Loureiro, Liliana R.; Carrascal, Mylène A.; Barbas, Ana; Ramalho, José S.; Novo, Carlos; Delannoy, Philippe; Videira, Paula A.

2015-01-01

The carbohydrate antigens Tn and sialyl-Tn (STn) are expressed in most carcinomas and usually absent in healthy tissues. These antigens have been correlated with cancer progression and poor prognosis, and associated with immunosuppressive microenvironment. Presently they are used in clinical trials as therapeutic vaccination, but with limited success due to their low immunogenicity. Alternatively, anti-Tn and/or STn antibodies may be used to harness the immune system against tumor cells. Whilst the development of antibodies against these antigens had a boost two decades ago for diagnostic use, so far no such antibody entered into clinical trials. Possible limitations are the low specificity and efficiency of existing antibodies and that novel antibodies are still necessary. The vast array of methodologies available today will allow rapid antibody development and novel formats. Following the advent of hybridoma technology, the immortalization of human B cells became a methodology to obtain human monoclonal antibodies with better specificity. Advances in molecular biology including phage display technology for high throughput screening, transgenic mice and more recently molecularly engineered antibodies enhanced the field of antibody production. The development of novel antibodies against Tn and STn taking advantage of innovative technologies and engineering techniques may result in innovative therapeutic antibodies for cancer treatment. PMID:26270678

9 CFR 77.9 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... TUBERCULOSIS Cattle and Bison § 77.9 Modified accredited advanced States or zones. (a) The following are... herd test requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication... reclassified as modified accredited. (d) If tuberculosis is diagnosed within a modified accredited advanced...

9 CFR 77.9 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... TUBERCULOSIS Cattle and Bison § 77.9 Modified accredited advanced States or zones. (a) The following are... apply the herd test requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis... being reclassified as modified accredited. (d) If tuberculosis is diagnosed within a modified accredited...

9 CFR 77.9 - Modified accredited advanced States or zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... TUBERCULOSIS Cattle and Bison § 77.9 Modified accredited advanced States or zones. (a) The following are... apply the herd test requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis... being reclassified as modified accredited. (d) If tuberculosis is diagnosed within a modified accredited...

Tn5Prime, a Tn5 based 5' capture method for single cell RNA-seq.

PubMed

Cole, Charles; Byrne, Ashley; Beaudin, Anna E; Forsberg, E Camilla; Vollmers, Christopher

2018-06-01

RNA-sequencing (RNA-seq) is a powerful technique to investigate and quantify entire transcriptomes. Recent advances in the field have made it possible to explore the transcriptomes of single cells. However, most widely used RNA-seq protocols fail to provide crucial information regarding transcription start sites. Here we present a protocol, Tn5Prime, that takes advantage of the Tn5 transposase-based Smart-seq2 protocol to create RNA-seq libraries that capture the 5' end of transcripts. The Tn5Prime method dramatically streamlines the 5' capture process and is both cost effective and reliable. By applying Tn5Prime to bulk RNA and single cell samples, we were able to define transcription start sites as well as quantify transcriptomes at high accuracy and reproducibility. Additionally, similar to 3' end-based high-throughput methods like Drop-seq and 10× Genomics Chromium, the 5' capture Tn5Prime method allows the introduction of cellular identifiers during reverse transcription, simplifying the analysis of large numbers of single cells. In contrast to 3' end-based methods, Tn5Prime also enables the assembly of the variable 5' ends of the antibody sequences present in single B-cell data. Therefore, Tn5Prime presents a robust tool for both basic and applied research into the adaptive immune system and beyond.

77 FR 19605 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Salmon

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-02

...-BB77 Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Salmon AGENCY: National Marine... exclusive economic zone (EEZ, 3 to 200 nautical miles) off Alaska are managed under the FMP. The FMP was... bycatch and bycatch mortality, (4) maximize economic and social benefits to the Nation over time, (5...

77 FR 21716 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Salmon

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-11

.... 120330244-2242-01] RIN 0648-BB77 Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Salmon AGENCY... commercial and sport fisheries that occur in the exclusive economic zone (EEZ) off Alaska. The FMP... bycatch and bycatch mortality, (4) maximize economic and social benefits to the Nation over time, (5...

77 FR 59994 - Nuclear Fuel Services, Inc., Erwin, TN; Issuance of License Renewal

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-01

... NUCLEAR REGULATORY COMMISSION [Docket No. 70-143; NRC-2009-0435] Nuclear Fuel Services, Inc., Erwin, TN; Issuance of License Renewal AGENCY: U.S. Nuclear Regulatory Commission. ACTION: Notice of... Nuclear Material Safety and Safeguards, U.S. Nuclear Regulatory Commission, Washington, DC 20555-0001...

77 FR 75570 - Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Salmon

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-21

.... 120330244-2673-02] RIN 0648-BB77 Fisheries of the Exclusive Economic Zone Off Alaska; Pacific Salmon AGENCY... the Pacific salmon that occur in the vast majority of the exclusive economic zone (EEZ) off Alaska...) maximize economic and social benefits to the Nation over time; (5) protect wild stocks and fully utilize...

Decision Making: An Imperative for Language Learning; Highlights of the Annual Language Arts Conference of Memphis State University (7th, Memphis, June 12-14, 1974).

ERIC Educational Resources Information Center

Rakes, Thomas A., Ed.; Brotherton, Sophia, Ed.

This document presents nine papers which were originally prepared for the 1974 Annual Language Arts Conference at Memphis State University. Included are: "Proxemics" by Dale F. Baltus; "Reading and Study Skill Hints for Intermediate and Secondary Teachers" by Stuart W. Bray; "A Reading Game License" by Flora C. Fowler; "Teach Kids to Think" by…

Urban Seismic Hazard Mapping for Memphis, Shelby County, Tennessee

USGS Publications Warehouse

Gomberg, Joan

2006-01-01

Earthquakes cannot be predicted, but scientists can forecast how strongly the ground is likely to shake as a result of an earthquake. Seismic hazard maps provide one way of conveying such forecasts. The U.S. Geological Survey (USGS), which produces seismic hazard maps for the Nation, is now engaged in developing more detailed maps for vulnerable urban areas. The first set of these maps is now available for Memphis, Tennessee.

The Levels and Distribution of TN, TP and TOC in the South China Sea

NASA Astrophysics Data System (ADS)

Wang, H.; Han, D.

2012-04-01

The marine biogeochemistries of carbon, nitrogen and phosphorous have come under increased scrutiny because of their close involvement in climate change and coastal eutrophication. The South China Sea is unique in that located in a subtropical zone, and therefore represents an important regime for biogeochemical studies. However, to our knowledge, few data are available for total nitrogen (TN), total phosphorous (TP) and total organic carbon (TOC) in South Sea, China. The present study aims to contribute to the knowledge of their status through investigating the level and distribution of TN, TP and TOC in South China Sea. A total of 108 seawater samples of 11 sites in south sea, china were collected during August 29- September 4, 2006. An automated and simultaneous method for determination of TN and TOC was applied to all seawater samples. The combined system allowed simultaneous determination for TOC and TN in the same sample using a single injection and provided low detection limits and excellent linear ranges for both TOC and TN. The risk of contamination has been remarkably reduced due to the minimal sample manipulation and automated analyses. And quantitative analyses of TP in seawater were accomplished by a typical chemical method. Concentration ranges of TN and TP were 0.06-0.67, and 0.003-0.071 mg/L, respectively, as well as that of TOC were 0.23-2.51mg/L. The values of TN and TP showed that the status of nutrition is relatively better in south china sea than other marine areas. Moreover, the upright change trend of TN concentration level as well as TP and TOC according to the experimental results at the total 11 sites are simultaneity studied. The concentration of TN initial increases with the increasing of the depth, later the value becomes almost constant. In contrast, the concentration of TOC reduces with the increasing of the depth, later the value becomes almost unchangeable. Compared with the trend of TN and TOC, that of TP appears relatively stable

Segregation by District Boundary Line: The Fragmentation of Memphis Area Schools

ERIC Educational Resources Information Center

Frankenberg, Erica; Siegel-Hawley, Genevieve; Diem, Sarah

2017-01-01

Boundary lines have long served as a mechanism to divide people, determining the quality of available resources, and ultimately, educational opportunity. In recent years, new school district boundaries have proliferated as local communities attempt to secede from larger school districts. In this study of Memphis-Shelby County, Tennessee, we extend…

Administrative Record Introduction, American Drum & Pallet Co. Removal Site, Memphis, Shelby County, Tennessee

EPA Pesticide Factsheets

Introduction to a document that contains the Index to and the Administrative Record for the American Drum & Pallet Co. Removal Site, Memphis, Shelby County, Tennessee. Region ID: 04 DocID: 10517014, DocDate: 08-17-2007

Negro Employment in the South. Volume 2: The Memphis Labor Market.

ERIC Educational Resources Information Center

Manpower Administration (DOL), Washington, DC.

This monograph on Negro employment in the Memphis labor market is part of a federally sponsored project on Negro employment in the South which seeks to present the latest information on both the racial employment patterns and the factors responsible for perpetuating or for changing those patterns. As part of a doctoral dissertation, a comparison…

75 FR 52367 - Notice of Inventory Completion: Memphis Pink Palace Museum, Memphis, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-25

... the Nodena, Parkin and Walls Phases of the Late Mississippian and proto-historic periods (A.D. 1350... culturally affiliated with the cultural assemblages fround on archeological sites related to Nodena, Parkin...

Changes in shallow groundwater quality beneath recently urbanized areas in the Memphis, Tennessee area

USGS Publications Warehouse

Barlow, Jeannie R.; Kingsbury, James A.; Coupe, Richard H.

2012-01-01

Memphis, the largest city in the state of Tennessee, and its surrounding suburbs depend on a confined aquifer, the Memphis aquifer, for drinking water. Concern over the potential for downward movement of water from an overlying shallow aquifer to the underlying Memphis aquifer provided impetus for monitoring groundwater quality within the shallow aquifer. The occurrence of volatile organic compounds (VOCs), nitrate, and pesticides in samples from the shallow well network indicate a widespread affect on water quality from the overlying urban land use. Total pesticide concentration was generally higher in more recently recharged groundwater indicating that as the proportion of recent water increases, the occurrence of pesticides related to the current urban land use also increases. Groundwater samples with nitrate concentrations greater than 1.5 mg/l and detectable concentrations of the pesticides atrazine and simazine also had higher concentrations of chloroform, a VOC primarily associated with urban land use, than in other samples. The age of the water from these wells indicates that these concentrations are most likely not representative of past agricultural use, but of more recent urban use of these chemicals. Given that the median age of water represented by the shallow well network was 21 years, a lag time likely exists between changes in land use and the occurrence of constituents related to urbanization in shallow groundwater.

Into the Second Century: Memphis Engineer District, 1976-1981

DTIC Science & Technology

1983-01-01

stream out of Lake Itasca in central Minnesota, the river begins a 2,340-mile journey to the Gulf of Mexico. In making the long journey, the river...McKellar Lake in honor of the senior Senator from Tennessee, Kenneth D. McKellar. Part of Tennessee Chute was dredged and then used as a slack...Missouri; and the Reelfoot -Obion areas in west Tennessee to monitor flood control structures. Under Phase I operations the Memphis District provided

39 CFR 221.6 - Field organization.

Code of Federal Regulations, 2011 CFR

2011-07-01

.... Pacific San Francisco CA. Southeast Memphis TN. Southwest Dallas TX. Western Denver CO. (c) Area functions...) Reporting relationships. Independent delivery distribution centers and post offices level EAS-24 and below...

Oxidized zirconium on ceramic; Catastrophic coupling.

PubMed

Ozden, V E; Saglam, N; Dikmen, G; Tozun, I R

2017-02-01

Oxidized zirconium (Oxinium™; Smith & Nephew, Memphis, TN, USA) articulated with polyethylene in total hip arthroplasty (THA) appeared to have the potential to reduce wear dramatically. The thermally oxidized metal zirconium surface is transformed into ceramic-like hard surface that is resistant to abrasion. The exposure of soft zirconium metal under hard coverage surface after the damage of oxidized zirconium femoral head has been described. It occurred following joint dislocation or in situ succeeding disengagement of polyethylene liner. We reported three cases of misuse of Oxinium™ (Smith & Nephew, Memphis, TN, USA) heads. These three cases resulted in catastrophic in situ wear and inevitable failure although there was no advice, indication or recommendation for this use from the manufacturer. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

77 FR 75016 - Safety Zone: Gilmerton Bridge Center Span Float-in, Elizabeth River; Norfolk, Portsmouth, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-19

...-AA00 Safety Zone: Gilmerton Bridge Center Span Float-in, Elizabeth River; Norfolk, Portsmouth, and... final rule establishing a safety zone around the Gilmerton Bridge center span barge. Inadvertently, this... Gilmerton Bridge center span barge (77 FR 73541). Inadvertently, this rule included an error in the...

76 FR 72047 - Enhanced-Use Lease (EUL) of Department of Veterans Affairs (VA) Real Property for the Development...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-21

... Hotel and General Use Retail Space in Memphis, TN AGENCY: Department of Veterans Affairs. ACTION: Notice... professional medical office building, a hotel, and general use retail space. FOR FURTHER INFORMATION CONTACT...

10. CUT OF THE CHURCH BUILDING AS IT APPEARED ON ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

10. CUT OF THE CHURCH BUILDING AS IT APPEARED ON THE PRINTED PROGRAM OF THE JANUARY 1, 1893 DEDICATION SERVICE. - Second Presbyterian Church, Pontatoc Avenue & Hernando Street, Memphis, Shelby County, TN

77 FR 5398 - Safety Zone; Atlantic Intracoastal Waterway, Vicinity of Marine Corps Base, Camp Lejeune, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-03

...-AA00 Safety Zone; Atlantic Intracoastal Waterway, Vicinity of Marine Corps Base, Camp Lejeune, NC... zone on the Atlantic Intracoastal Waterway (AICW) adjacent to Marine Corps Base (MCB) Camp Lejeune..., Vicinity of Marine Corps Base, Camp Lejeune, NC in the Federal Register (77 FR 1431). We received no...

The Nutritional Status of Preschool Children in a Memphis Innercity Area. Report.

ERIC Educational Resources Information Center

Zee, Paul; DeLeon, Marina

Preschool children from poor families in a black Memphis community, who had shown considerable nutritional progress since a food program was introduced in 1969, lost some of these gains during the 1974-1976 recession. These losses can be attributed to inflation and the high rate of unemployment among the blacks in this community. The unfavorable…

77 FR 73541 - Safety Zone: Gilmerton Bridge Center Span Float-in, Elizabeth River; Norfolk, Portsmouth, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-11

...-AA00 Safety Zone: Gilmerton Bridge Center Span Float-in, Elizabeth River; Norfolk, Portsmouth, and... Gilmerton Bridge Center Span Float- in and bridge construction of span placement. This action is intended to... rulemaking (NPRM) proposing a safety zone in the Gilmerton Bridge Area (77 FR 43557) on September 5-9, 2012...

Preliminary evaluation of flood frequency relations in the urban areas of Memphis, Tennessee

USGS Publications Warehouse

Boning, Charles W.

1977-01-01

A storm-runoff relation for streams in the urban areas of Memphis was determined by a statistical evaluation of 59 flood discharges from 19 gaging stations. These flood discharges were related to drainage area, percent imperviousness of the drainage basin, and rainfall occuring over 120-minute periods. The defined relation is Q=m3A*777A - .02 tI,,,,P + 1j-227 (1120).539(t120).40 where Q is flood discharge in cfs, A is drainage area in square miles, IMP is percent imperviousness in the basin, and I120 is rainfall in inches, over 120 minute time period. The defined relation was used to synthesize sets of annual flood peaks for drainage basins ranging from .05 square miles to 10 square miles and imperviousness ranging from 0 to 80 percent for the period of rainfall record at Memphis. From these series of flood peaks, frequency relations were defined and presented for 2, 5, 10, 25, 50 and 100 year recurrent intervals.

EnviroAtlas - Memphis, TN - Near Road Tree Buffer

EPA Pesticide Factsheets

This EnviroAtlas dataset addresses the tree buffer along heavily traveled roads. The roads are interstates, arterials, and collectors within the EnviroAtlas community boundary. Forest is defined as Trees & Forest and Woody Wetlands. Sufficient tree bufferage is defined as 25% coverage within the circular moving window with a radius of 14.5m at any given point along the roadway. There are potential negative health effects for those living in a location without a sufficient tree buffer. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

EnviroAtlas - Memphis, TN - Green Space Proximity Gradient

EPA Pesticide Factsheets

In any given 1-square meter point in this EnviroAtlas dataset, the value shown gives the percentage of square meters of greenspace within 1/4 square kilometer centered over the given point. Green space is defined as Trees & Forest, Grass & Herbaceous, Agriculture, Woody Wetlands, and Emergent Wetlands. Water is shown as -99999 in this dataset to distinguish it from land areas with very low green space. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

Holistic Health Care for the Medically Uninsured: The Church Health Center of Memphis.

PubMed

Morris, G Scott

2015-11-05

The Church Health Center (CHC) in Memphis was founded in 1987 to provide quality, affordable health care for working, uninsured people and their families. With numerous, dedicated financial supporters and health care volunteers, CHC has become the largest faith-based health care organization of its type nationally, serving >61,000 patients. CHC embraces a holistic approach to health by promoting wellness in every dimension of life. It offers on-site services including medical care, dentistry, optometry, counseling, social work, and nutrition and fitness education, to promote wellness in every dimension of life. A 2012 economic analysis estimated that a $1 contribution to the CHC provided roughly $8 in health services. The CHC has trained >1200 Congregational Health Promoters to be health leaders and is conducting research on the effectiveness of faith community nurses partnering with congregations to assist in home care for patients recently discharged from Memphis hospitals. The MEMPHIS Plan, CHC's employer-sponsored health care plan for small business and the self-employed, offers uninsured people in lower-wage jobs access to quality, affordable health care. The CHC also conducts replications workshops several times a year to share their model with leaders in other communities. The Institute for Healthcare Improvement (IHI) recently completed a case study that concluded: "The CHC is one of a very few organizations successfully embodying all three components of the IHI Triple Aim by improving population health outcomes, enhancing the individual's health care experience, and controlling costs. All three have been part of the Center's DNA since its inception, and as a transforming force in the community, the model is well worth national attention."

Memphis State University's New Five-year Program For the Initial Preparation of Teachers.

ERIC Educational Resources Information Center

Saunders, Robert L.

A description is presented of a new extended teacher education program at Memphis State University. Instituted in 1985, the program has four objectives: (1) to develop and implement an extended (five-year) program covering all areas of teacher preparation, with institutional recommendation for certification coming only after completion of the…

78 FR 51721 - Notice of Receipt of Requests To Voluntarily Cancel Certain Pesticide Registrations

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-21

... Marine Premium Morpholine, 4,4'-(2-ethyl- Diesel With Bioguard 2-nitro-1,3- Additive. propanediyl)bis-, 4... Triangle Park, NC 27709. 1022 IBC Manufacturing Co., 416 E. Brooks Road, Memphis, TN 38109. 9688 Chemsico...

75 FR 61696 - Foreign-Trade Zone 148-Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-06

...--Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon Fiber and Oxidized..., requesting special-purpose subzone status for the carbon fiber and oxidized polyacrylonitrile fiber (OPF...)--based carbon fiber and OPF (up to 4,000 metric tons combined annually) for export and the domestic...

77 FR 44582 - Reorganization of Foreign-Trade Zone 183 Under Alternative Site Framework; Austin, TX

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-30

... categorized as magnet sites; Whereas, notice inviting public comment was given in the Federal Register (77 FR... the overall general-purpose zone project, and to a five-year ASF sunset provision for magnet sites...

Water Levels and Selected Water-Quality Conditions in the Sparta-Memphis Aquifer (Middle Claiborne Aquifer) in Arkansas, Spring-Summer 2007

USGS Publications Warehouse

Schrader, T.P.

2009-01-01

The U.S. Geological Survey in cooperation with the Arkansas Natural Resources Commission and the Arkansas Geological Survey has monitored water levels in the Sparta Sand of Claiborne Group and Memphis Sand of Claiborne Group (herein referred to as the Sparta Sand and the Memphis Sand, respectively), since the 1920s. Groundwater withdrawals have increased while water levels have declined since monitoring was initiated. Herein, aquifers in the Sparta Sand and Memphis Sand will be referred to as the Sparta-Memphis aquifer throughout Arkansas. During the spring of 2007, 309 water levels were measured in wells completed in the Sparta-Memphis aquifer. During the summer of 2007, 129 water-quality samples were collected and measured for temperature and specific conductance and 102 were collected and analyzed for chloride from wells completed in the Sparta-Memphis aquifer. Water-level measurements collected in wells screened in the Sparta-Memphis aquifer were used to produce a regional potentiometric-surface map. The regional direction of groundwater flow in the Sparta-Memphis aquifer is generally to the south-southeast in the northern half of Arkansas and to the east and south in the southern half of Arkansas, away from the outcrop area except where affected by large ground-water withdrawals. The highest water-level altitude measured in the Sparta-Memphis aquifer was 326 feet above National Geodetic Vertical Datum of 1929, located in Grant County in the outcrop at the western boundary of the study area; the lowest water-level altitude was 161 feet below National Geodetic Vertical Datum of 1929 in Union County near the southern boundary of the study area. Eight cones of depression (generally represented by closed contours) are located in the following counties: Bradley, Drew, and Ashley; Calhoun; Cleveland; Columbia; Crittenden; Arkansas, Jefferson, and Lincoln; Cross and Poinsett; and Union. Two large depressions are shown on the 2007 potentiometric-surface map, centered

78 FR 64938 - Product Cancellation Order for Certain Pesticide Registrations

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-30

..., Premium Diesel 4,4[min]-(2-ethyl- With Bioguard 2-nitro-1,3- Additive. propanediyl)bis-, 4-(2- Nitrobutyl... Triangle Park, NC 27709. 1022 IBC Manufacturing Co., 416 E. Brooks Rd., Memphis, TN 38109. 9688 Chemsico, A...

77 FR 40798 - Safety Zone; Nautical City Festival Air Show, Rogers City MI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-11

...-AA00 Safety Zone; Nautical City Festival Air Show, Rogers City MI AGENCY: Coast Guard, DHS. ACTION...; Nautical City Festival Air Show, Rogers City MI; in the Federal Register (77 FR 29932). We received no... Nautical City Festival will be celebrating Calcite's 100th Anniversary. As part of that celebration, an air...

School Segregation and Its Discontents: Chaos and Community in Post-Civil Rights Memphis

ERIC Educational Resources Information Center

Rushing, Wanda

2017-01-01

Few policies have affected American society as deeply as those related to the landmark 1954 U.S. Supreme Court decision, "Brown v. Board of Education." Now, 60 years later, segregation persists along race and class divisions. This case study analysis of a merger that took place between 2010 and 2013 in Memphis and Shelby County,…

Evidence for Environmental Contamination in Residential Neighborhoods Surrounding the Defense Depot of Memphis, Tennessee

PubMed Central

Greene, Natasha A.; White, Jason D.; Morris, Vernon R.; Roberts, Stephanie; Jones, Kimberly L.; Warrick, Cynthia

2006-01-01

An interdisciplinary environmental assessment team from the Howard University Environmental Justice Partnership (HUEJP) conducted a site visit and assessment of the Defense Depot of Memphis, Tennessee in February of 2000. This depot was built in the late 1940’s for storage of numerous chemicals and munitions. As the years progressed, many Memphis citizens have grown to believe that the activities and chemical stockpile located at this site have negatively affected the health environment of their residents. There is anecdotal evidence and documentation of numerous cancers and other illnesses in those local territories, and specifically, at the Memphis Depot site. Currently, this depot is closed and in remediation by the local government. Particularly, citizens of the Rozelle community have started a campaign to investigate any signs of exposure pathways to noted health risks. The HUEJP was contacted and asked to investigate the community concerns. Obliging to the request, we aimed to sample at three drainage sites and a residential site, talk to local citizens, and gain any additional information that would be helpful in relieving anxiety in the Rozelle community. Soil, sediment, and water samples were collected and analyzed for total organic carbon, inorganic anions, and heavy metals. These data show that for the four sites sampled, the highest concentrations of organic compounds and heavy metals were located either within a residential area or in an area with a direct transport pathway to the community. Atomic absorption analysis revealed detectable amounts of cadmium, lead and chromium metals at all sites with direct transport pathways into the residential community, with chromium concentrations being far in excess of the EPA standard limits. PMID:16968970

77 FR 54558 - Reorganization of Foreign-Trade Zone 189 Under Alternative Site Framework, Kent, Ottawa, and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-05

... categorized as magnet sites; Whereas, notice inviting public comment was given in the Federal Register (77 FR... general-purpose zone project, and to a five-year ASF sunset provision for magnet sites that would...

Microbial respiration and extracellular enzyme activity in sediments from the Gulf of Mexico hypoxic zone

EPA Science Inventory

This study explores the relationship between sediment chemistry (TC, TN, TP) and microbial respiration (DHA) and extracellular enzyme activity (EEA) across the Gulf of Mexico (GOM) hypoxic zone. TC, TN, and TP were all positively correlated with each other (r=0.19-0.68). DHA was ...

Tn552 transposase purification and in vitro activities.

PubMed Central

Rowland, S J; Sherratt, D J; Stark, W M; Boocock, M R

1995-01-01

The Staphylococcus aureus transposon Tn552 encodes a protein (p480) containing the 'D,D(35)E' motif common to retroviral integrases and the transposases of a number of bacterial elements, including phage Mu, the integron-containing element Tn5090, Tn7 and IS3. p480 and a histidine-tagged derivative were overexpressed in Escherichia coli and purified by methods involving denaturation and renaturation. DNase I footprinting and gel binding assays demonstrated that p480 binds to two adjacent, directly repeated 23 bp motifs at each end of Tn552. Although donor strand cleavage by p480 was not detected, in vitro conditions were defined for strand transfer activity with transposon end fragments having pre-cleaved 3' termini. Strand transfer was Mn(2+)-dependent and appeared to join a single left or right end fragment to target DNA. The importance of the terminal dinucleotide CA-3' was demonstrated by mutation. The in vitro activities of p480 are consistent with its proposed function as the Tn552 transposase. Images PMID:7828593

Annotated Administrative Record Site-Specific Document Index, American Drum & Pallet Co. Removal Site, Memphis, Shelby County, Tennessee

EPA Pesticide Factsheets

Contains annotated index of site specific documents for the American Drum & Pallet Co. Removal Site in Memphis, Shelby County, Tennessee, January 9, 2008 Region ID: 04 DocID: 10517016, DocDate: 01-09-2008

Potassium Iodide ("KI"): Instructions to Make Potassium Iodide Solution for Use During a Nuclear Emergency (Liquid Form)

MedlinePlus

... high capacity to mask the saltiness while low fat milk has little capacity to cover the saltiness of KI. This work was completed through a FDA contract with the Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee, Memphis, TN ...

75 FR 63177 - Availability of FY 09 Grantee Performance Evaluation Reports for the Eight States of EPA Region 4...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-14

...-year evaluations of eight state air pollution control programs (Alabama Department of Environmental... Management, FL; Louisville Metro Air Pollution Control District, KY; Forsyth County Environmental Affairs... Regional Air Quality Agency, NC; Chattanooga-Hamilton County Air Pollution Control Bureau, TN; Memphis...

Space Maintenance and New Bone Formation with Polyurethane Biocomposites in a Canine Saddle Defect

DTIC Science & Technology

2014-05-01

Vanderbilt University, Nashville, TN 2. Medtronic Spinal and Biologics, Memphis, TN 3. US Army Institute of Surgical Research, Fort Sam Houston, TX...and 15% hydroxyapatite (HA) that is similar in mineral content to natural bone.3 45S5 Bioactive glass (BG) is a resorbable material that has been... used effectively in a variety of bone regeneration applications.4 In the present study, we investigated the ability of injectable PUR/MG and PUR/BG

Emotional Responses to the Linguistic Landscape in Memphis, Tennessee: Visual Perceptions of Public Spaces in Transition

ERIC Educational Resources Information Center

Garvin, Rebecca Todd

2011-01-01

This qualitative ethnographic Linguistic Landscape (LL) study collected and analyzed ten individual "walking tour" interviews with residents of Memphis, Tennessee, exploring the personal thoughts and feelings about linguistic changes in the communities triggered by the LL. The researcher focused the participants' attention on…

Water levels and water quality in the Sparta-Memphis aquifer (middle Claiborne aquifer) in Arkansas, spring-summer 2009

USGS Publications Warehouse

Schrader, T.P.

2013-01-01

The U.S. Geological Survey in cooperation with the Arkansas Natural Resources Commission and the Arkansas Geological Survey has monitored water levels in the Sparta Sand of Claiborne Group and Memphis Sand of Claiborne Group (herein referred to as the Sparta Sand and the Memphis Sand, respectively) since the 1920s. Groundwater withdrawals have increased while water levels have declined since monitoring was initiated. Herein, aquifers in the Sparta Sand and Memphis Sand will be referred to as the Sparta-Memphis aquifer throughout Arkansas. During the spring of 2009, 324 water levels were measured in wells completed in the Sparta-Memphis aquifer and used to produce a regional potentiometric-surface map. During the summer of 2009, 64 water-quality samples were collected and measured for specific conductance, temperature, and pH from wells completed in the Sparta-Memphis aquifer. The regional direction of groundwater flow in the Sparta-Memphis aquifer is generally to the south-southeast in the northern half of Arkansas and to the east and south in the southern half of Arkansas, away from the outcrop area except where affected by large groundwater withdrawals. The highest and lowest water-level altitudes measured in the Sparta-Memphis aquifer were 325 feet above and 157 feet below National Geodetic Vertical Datum of 1929, respectively. Eight depressions (generally represented by closed contours) are located in the following counties: Bradley; Ashley; Calhoun; Cleveland; Columbia; Arkansas, Jefferson, Lincoln, and Prairie; Cross and Poinsett; and Union. Two large depressions shown on the 2009 potentiometric-surface map, centered in Jefferson and Union Counties, are the result of large withdrawals for industrial, irrigation, or public supply. The depression centered in Jefferson County deepened and expanded in recent years into Arkansas and Prairie Counties. The area enclosed within the 40-foot contour on the 2009 potentiometric-surface map has expanded south to the Drew

EnviroAtlas - Memphis, TN - Near Road Block Group Summary

EPA Pesticide Factsheets

This EnviroAtlas dataset addresses the tree buffer along heavily traveled roads. The roads are interstates, arterials, and collectors within the EnviroAtlas community boundary. Forest is defined as Trees & Forest and Woody Wetlands. Sufficient tree bufferage is defined as 25% coverage within the circular moving window with a radius of 14.5m at any given point along the roadway. There are potential negative health effects for those living in a location without a sufficient tree buffer. Those populations are estimated here using dasymetric data calculated for the EnviroAtlas. There are potential negative health effects for those living in a location without a sufficient tree buffer. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

Preliminary Memphis FAA (Federal Aviation Administration)/Lincoln Laboratory Operational Weather Studies Results.

DTIC Science & Technology

1987-04-22

Spatial distribution of windshear events in the mid-South. Each point indicates the approximate initial location of a microburst, macroburst or...rapidly to become " macrobursts " with gust fronts at the outflow edges. There appeared* to be fewer microbursts in Memphis then in other areas previously...34 macrobursts " because they were larger than 4 km across. Some of these we also labeled "divergent areas" in the radar log, sometimes because we were reluctant

Tn5401, a new class II transposable element from Bacillus thuringiensis.

PubMed Central

Baum, J A

1994-01-01

A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, in the same orientation, encoding proteins of 36 kDa (306 residues) and 116 kDa (1,005 residues) and 53-bp terminal inverted repeats. The deduced amino acid sequence for the 36-kDa protein shows 24% sequence identity with the TnpI recombinase of the B. thuringiensis transposon Tn4430, a member of the phage integrase family of site-specific recombinases. The deduced amino acid sequence for the 116-kDa protein shows 42% sequence identity with the transposase of Tn3 but only 28% identity with the TnpA transposase of Tn4430. Two small open reading frames of unknown function, designated orf1 (85 residues) and orf2 (74 residues), were also identified. Southern blot analysis indicated that Tn5401, in contrast to Tn4430, is not commonly found among different subspecies of B. thuringiensis and is not typically associated with known insecticidal crystal protein genes. Transposition was studied with B. thuringiensis by using plasmid pEG922, a temperature-sensitive shuttle vector containing Tn5401. Tn5401 transposed to both chromosomal and plasmid target sites but displayed an apparent preference for plasmid sites. Transposition was replicative and resulted in the generation of a 5-bp duplication at the target site. Transcriptional start sites within Tn5401 were mapped by primer extension analysis. Two promoters, designated PL and PR, direct the transcription of orf1-orf2 and tnpI-tnpA, respectively, and are negatively regulated by TnpI. Sequence comparison of the promoter regions of Tn5401 and Tn4430 suggests that the conserved sequence element ATGTCCRCTAAY mediates TnpI binding and cointegrate resolution. The same element is contained within the 53-bp terminal

Historic water-level changes and pumpage from the principal aquifers of the Memphis area, Tennessee: 1886-1975

USGS Publications Warehouse

Criner, James H.; Parks, William Scott

1976-01-01

Pumpage from the Fort Pillow Sand ("1,400-foot" sand) began in 1924 and increased at a yearly rate of about 0.6 Mgal/d (2.3 Ml/d) until 1942. From 1943 to 1962, pumpage averaged about 11.5 Mgal/d (43.5 Ml/d), then was reduced as MLGW (Memphis Light, Gas and Water Division) discontinued wells that became unserviceable. MLGW ceased pumping from the aquifer in 1974, and pumpage from the remaining industrial wells in Shelby County in 1975 was 4.4 Mgal/d (16.6 Ml/d). Water levels in the Fort Pillow Sand generally have risen since 1963. Water l evels in the aquifers in the Memphis area fluctuate inversely with changes in pumping. Analysis of observation-well and pumpage data indicates that local water levels can be altered by changing the pumping rates or by varying the areal distribution of pumping.

Spatiotemporal patterns of childhood asthma hospitalization and utilization in Memphis Metropolitan Area from 2005 to 2015.

PubMed

Oyana, Tonny J; Podila, Pradeep; Wesley, Jagila Minso; Lomnicki, Slawo; Cormier, Stephania

2017-10-01

To identify the key risk factors and explain the spatiotemporal patterns of childhood asthma in the Memphis metropolitan area (MMA) over an 11-year period (2005-2015). We hypothesize that in the MMA region this burden is more prevalent among urban children living south, downtown, and north of Memphis than in other areas. We used a large-scale longitudinal electronic health record database from an integrated healthcare system, Geographic information systems (GIS), and statistical and space-time models to study the spatiotemporal distributions of childhood asthma at census tract level. We found statistically significant spatiotemporal clusters of childhood asthma in the south, west, and north of Memphis city after adjusting for key covariates. The results further show a significant increase in temporal gradient in frequency of emergency department (ED) visits and inpatient hospitalizations from 2009 to 2013, and an upward trajectory from 4 per 1,000 children in 2005 to 16 per 1,000 children in 2015. The multivariate logistic regression identified age, race, insurance, admit source, encounter type, and frequency of visits as significant risk factors for childhood asthma (p < 0.05). We observed a greater asthma burden and healthcare utilization for African American (AA) patients living in a high-risk area than those living in a low-risk area in comparison to the white patients: AA vs. white [odds ratio (OR) = 3.03, 95% confidence interval (CI): 2.75-3.34]; and Hispanic vs. white (OR = 1.62, 95% CI: 1.21-2.17). These findings provide a strong basis for developing geographically tailored population health strategies at the neighborhood level for young children with chronic respiratory conditions.

International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21▿

PubMed Central

Novais, Ângela; Baquero, Fernando; Machado, Elisabete; Cantón, Rafael; Peixe, Luísa; Coque, Teresa M.

2010-01-01

TEM-24 remains one of the most widespread TEM-type extended-spectrum β-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-ΔTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly

77 FR 46613 - Safety Zone; 2012 Ironman US Championship Swim, Hudson River, Fort Lee, NJ

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-06

... 1625-AA00 Safety Zone; 2012 Ironman US Championship Swim, Hudson River, Fort Lee, NJ AGENCY: Coast... navigable waters of the Hudson River in the vicinity of Englewood Cliffs and Fort Lee, NJ for the 2012... Championship Swim, Hudson River, Fort Lee, NJ in the Federal Register (77 FR 34285). We received no comments on...

76 FR 24914 - Notice of Determinations Regarding Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-03

.... 75,003 Velsicol Chemical, LLC, Memphis, TN December 15, 2009. True Specialties Corp., Quarles..., TX November 1, 2009. SQA Engineering, VCS and VCS-One Group. 74,810A Symantec Corporation, Mountain View, CA..... November 1, 2009. SQA Engineering, VCS and VCS-One Group. 74,810B Symantec Corporation...

Surficial geologic map of the southwest Memphis Quadrangle, Shelby County, Tennessee, and Crittenden County, Arkansas

USGS Publications Warehouse

Moore, David W.; Diehl, Sharon F.

2004-01-01

This map is one of seven 1:24,000-scale (7.5-minute) quadrangle maps of the surficial geology of the Memphis, Tennessee, area--part of a series of urban hazard maps. Wind-deposited silt and clayey silt (loess) is the predominant surficial deposit in this quadrangle. The loess was deposited as dust during the last major continental glaciation of the region and it covers the upland to depths of 4.5-16 m. River alluvium (unit Qal), which is chiefly a sandy and gravelly sand deposit about 30 m thick, underlies the Mississippi River floodplain. This unit supports extensive artificial fill and infrastructure used for shipping storage and petroleum processing and storage. Based on paleoliquefaction structures (sand boils) documented in Mississippi River alluvium elsewhere, this unit probably has the potential to liquefy during strong earthquake shaking. No paleoliquefaction structures were observed within the Southwest Memphis quadrangle. Another deposit in the quadrangle is silty alluvium of the Nonconnah Creek floodplain, and is 1-10 m thick. Sparse, unconsolidated pebbly sand deposits are 0.5-3 m thick and make up point bars and channel deposits of Nonconnah Creek.

75 FR 52364 - Notice of Intent To Repatriate Cultural Items: Memphis Pink Palace Museum, Memphis, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-25

... Spanish account, and a late 17th century Quapaw Indian village name ``Kappah'' or ``Kappa.'' French maps..., including hunting lands, and that burial practices such as placement of food with the dead continues to be...

76 FR 4532 - Safety Zone; 500 Yards North and South, Bank to Bank, of Position 29°48.77′ N 091°33.02′ W...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-26

...-AA00 Safety Zone; 500 Yards North and South, Bank to Bank, of Position 29[deg]48.77' N 091[deg]33.02' W... and South, bank to bank, of position 29[deg]48.77' N 091[deg]33.02' W, Charenton Drainage and Navigation Canal, St. Mary Parish, LA. This Safety Zone is needed to protect the general public, vessels and...

Analytical evaluation of point of care cTnT and clinical performances in an unselected population as compared with central laboratory highly sensitive cTnT.

PubMed

Dupuy, Anne Marie; Sebbane, Mustapha; Roubille, François; Coste, Thibault; Bargnoux, Anne Sophie; Badiou, Stéphanie; Kuster, Nils; Cristol, Jean Paul

2015-03-01

To report the analytical performances of the Radiometer AQT90 FLEX® cTnT assay (Neuilly-Plaisance, France) and to evaluate the concordance with hs-cTnT results from central laboratory for the diagnosis of acute myocardial infarction (AMI) at baseline and during a short follow-up among unselected patients admitted in emergency room or cardiology department. Analytical performances of AQT90 FLEX® cTnT immunoassay included imprecision study with determination of a coefficient of variation at 10% and 20%, linearity, and limit of detection. The concordance study was based on samples obtained from 170 consecutive patients with chest pain suggestive of acute coronary syndrome (ACS) admitted in the emergency room or cardiology department. The kinetic study (within 62 additional samples 3h later) was based on absolute delta criterion and the combination of relative change of 30% with absolute change of 7ng/L. The cTnT assay from Radiometer was evaluated as clinically usable, although less sensitive than the Roche hs-cTnT assay as demonstrated by the concordance and the kinetic studies. In non-selected population, the cTnT AQT Flex© assay on AQT90© with kinetic change at 3h, provides similar clinical classification of patients, particularly for AMI group as compared to central laboratory hs-cTnT assay and could be suitable for clinical use. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Tn4556, a 6.8-kilobase-pair transposable element of Streptomyces fradiae.

PubMed Central

Chung, S T

1987-01-01

A 6.8-kilobase-pair (kbp) transposable element (Tn4556) was found in a neomycin-producing strain of Streptomyces fradiae. This element was first observed in two 30.3-kbp plasmids (pUC1123 and pUC1124) which arose when a thiostrepton resistance gene (1 kbp) was ligated with the BclI-2 fragment (22.5 kbp) that contains the origin of replication of phage SF1. The Tn4556 segment was deleted when these plasmids were transduced into another S. fradiae host with phage SF1. These deletion plasmids (pUC1210 and pUC1211) had copy numbers of less than 1 per chromosome and were unstable. In contrast, pUC1123 and pUC1124, with copy numbers of 12 to 15 per chromosome, respectively, were relatively stable. When pUC1210 and pUC1211 were reintroduced into S. fradiae by protoplast transformation, the Tn4556 element transposed again to the plasmids at numerous new locations in either of two orientations. A copy of Tn4556 was found in the S. fradiae chromosome by hybridization studies. It appears that Tn4556 originated from the chromosome, transposed into unstable pUC1210 and pUC1211, and made stable plasmids. A temperature-sensitive hybrid plasmid carrying a viomycin resistance derivative of Tn4556 (pMT660::Tn4556::vph) was constructed. When Streptomyces lividans UC8390 containing the hybrid plasmid was grown at 39 degrees C, Tn4556::vph (Tn4560) transposed to random positions in the host chromosome. Images PMID:2820925

Mini-Tn7 Insertion in Bacteria With Multiple glmS-Linked attTn7 Sites: Example Burkholderia Mallei ATCC 23344

DTIC Science & Technology

2006-06-27

INTRODUCTION Burkholderia mallei is the etiological agent of glanders and a highly evolved obligate zoonotic mammalian pathogen that naturally affects horses...mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344 Kyoung-Hee Choi1, David DeShazer2... glanders is a rare disease, B. mallei has received renewed attention because of its listing as a category B agent by the Centers for Disease Control

Occurrence and properties of composite transposon Tn2672: evolution of multiple drug resistance transposons.

PubMed Central

Hänni, C; Meyer, J; Iida, S; Arber, W

1982-01-01

We found Tn2671 (the 23-kb long IS1-flanked r-determinant of NR1-Basel) inserted into the ampicillin resistance gene bla of the Tn3-related transposon Tn902. The resulting 28-kilobase-long composite transposon Tn2672 (= Tn902 bla::Tn2671) is stable, and it translocates as a unit into various loci including IS1 of the resistance transfer factor of R100-1. These results are discussed with respect to the evolution of R plasmids providing multiple drug resistance. Images PMID:6281241

Information Retrieval Center: A Proposal for the Implementation of CD-ROM Database Technology at Memphis State University Libraries.

ERIC Educational Resources Information Center

Evans, John; Park, Betsy

This planning proposal recommends that Memphis State University Libraries make information on CD-ROM (compact disc--read only memory) available in the Reference Department by establishing an Information Retrieval Center (IRC). Following a brief introduction and statement of purpose, the library's databases, users, staffing, facilities, and…

Tn5099, a xylE promoter probe transposon for Streptomyces spp.

PubMed Central

Hahn, D R; Solenberg, P J; Baltz, R H

1991-01-01

Tn5099, a promoter probe transposon for Streptomyces spp., was constructed by inserting a promoterless xylE gene and a hygromycin resistance gene into IS493. Tn5099 transposed into different sites in the Streptomyces griseofuscus genome, and the xylE reporter gene was expressed in some of the transposition mutants. Strains containing Tn5099 insertions that gave regulated expression of the xylE gene were identified. Images PMID:1653213

Expanded Learning, Expanded Opportunity: How Four Communities Are Working to Improve Education for Their Students

ERIC Educational Resources Information Center

America's Promise Alliance, 2015

2015-01-01

This report analyzes case studies of "more and better learning time" efforts around the country. With support from the Ford Foundation, America's Promise Alliance looked in Grand Rapids, MI; Louisville, KY; Memphis, TN; and Rochester, NY to see how time spent out of the classroom improved outcomes for students in low-income neighborhoods…

Role of Inflammation in MPTP-Induced Dopaminergic Degeneration

DTIC Science & Technology

2005-12-01

8217Department of Developmental Neurohiolog/, Saint Jude Children k Research Hospital, Memphis, TN 39105. USA "tNeuroscience Research Laboratories qf...astrucytes. J. Neuror’heo,. 68. 2216-22)9. Lannsion 1WV, LS Porno . J Te~trud, AG Reeves. JA Kaplan and 1) Lichent C J Arsaur. [R Vaulter and J Dernores

77 FR 50373 - Special Local Regulation and Safety Zone; America's Cup World Series Regattas, San Francisco Bay...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-21

...] RIN 1625-AA00; 1625-AA08 Special Local Regulation and Safety Zone; America's Cup World Series Regattas... Acronyms APA Administrative Procedure Act ACRM America's Cup Race Management DHS Department of Homeland... associated with the ``2012 America's Cup World Series'' regatta scheduled to occur August 21-26, 2012 (77 FR...

EnviroAtlas - Memphis, TN - 51m Riparian Buffer Vegetated Cover

EPA Pesticide Factsheets

This EnviroAtlas dataset describes the percentage of a 51-m riparian buffer that is vegetated. Vegetated cover is defined as Trees & Forest, Grass & Herbaceous, Woody Wetlands, and Emergent Wetlands. There is a potential for decreased water quality in areas where the riparian buffer is less forested. The displayed line represents the center of the analyzed riparian buffer. The water bodies analyzed include hydrologically connected streams, rivers, connectors, reservoirs, lakes/ponds, ice masses, washes, locks, and rapids within the EnviroAtlas community area. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

EnviroAtlas - Memphis, TN - 51m Riparian Buffer Forest Cover

EPA Pesticide Factsheets

This EnviroAtlas dataset describes the percentage of a 51-m riparian buffer that is forested. Forest is defined as Trees & Forest and Woody Wetlands. There is a potential for decreased water quality in areas where the riparian buffer is less forested. The displayed line represents the center of the analyzed riparian buffer. The water bodies analyzed include hydrologically connected streams, rivers, connectors, reservoirs, lakes/ponds, ice masses, washes, locks, and rapids within the EnviroAtlas community area. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

EnviroAtlas - Memphis, TN - Estimated Intersection Density of Walkable Roads

EPA Pesticide Factsheets

This EnviroAtlas dataset estimates the intersection density of walkable roads within a 750 meter radius of any given 10 meter pixel in the community. Intersections are defined as any point where 3 or more roads meet and density is calculated using kernel density, where closer intersections are weighted higher than further intersections. Intersection density is highly correlated with walking for transportation. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

An insight into the thermodynamic characteristics of human thrombopoietin complexation with TN1 antibody

PubMed Central

Shibazaki, Chie; Adachi, Motoyasu; Honjo, Eijiro; Tamada, Taro; Maeda, Yoshitake; Tahara, Tomoyuki; Kato, Takashi; Miyazaki, Hiroshi; Blaber, Michael; Kuroki, Ryota

2016-01-01

Abstract Human thrombopoietin (hTPO) primarily stimulates megakaryocytopoiesis and platelet production and is neutralized by the mouse TN1 antibody. The thermodynamic characteristics of TN1 antibody–hTPO complexation were analyzed by isothermal titration calorimetry (ITC) using an antigen‐binding fragment (Fab) derived from the TN1 antibody (TN1‐Fab). To clarify the mechanism by which hTPO is recognized by TN1‐Fab the conformation of free TN1‐Fab was determined to a resolution of 2.0 Å using X‐ray crystallography and compared with the hTPO‐bound form of TN1‐Fab determined by a previous study. This structural comparison revealed that the conformation of TN1‐Fab does not substantially change after hTPO binding and a set of 15 water molecules is released from the antigen‐binding site (paratope) of TN1‐Fab upon hTPO complexation. Interestingly, the heat capacity change (ΔCp) measured by ITC (−1.52 ± 0.05 kJ mol−1 K−1) differed significantly from calculations based upon the X‐ray structure data of the hTPO‐bound and unbound forms of TN1‐Fab (−1.02 ∼ 0.25 kJ mol−1 K−1) suggesting that hTPO undergoes an induced‐fit conformational change combined with significant desolvation upon TN1‐Fab binding. The results shed light on the structural biology associated with neutralizing antibody recognition. PMID:27419667

First USGS urban seismic hazard maps predict the effects of soils

USGS Publications Warehouse

Cramer, C.H.; Gomberg, J.S.; Schweig, E.S.; Waldron, B.A.; Tucker, K.

2006-01-01

Probabilistic and scenario urban seismic hazard maps have been produced for Memphis, Shelby County, Tennessee covering a six-quadrangle area of the city. The nine probabilistic maps are for peak ground acceleration and 0.2 s and 1.0 s spectral acceleration and for 10%, 5%, and 2% probability of being exceeded in 50 years. Six scenario maps for these three ground motions have also been generated for both an M7.7 and M6.2 on the southwest arm of the New Madrid seismic zone ending at Marked Tree, Arkansas. All maps include the effect of local geology. Relative to the national seismic hazard maps, the effect of the thick sediments beneath Memphis is to decrease 0.2 s probabilistic ground motions by 0-30% and increase 1.0 s probabilistic ground motions by ???100%. Probabilistic peak ground accelerations remain at levels similar to the national maps, although the ground motion gradient across Shelby County is reduced and ground motions are more uniform within the county. The M7.7 scenario maps show ground motions similar to the 5%-in-50-year probabilistic maps. As an effect of local geology, both M7.7 and M6.2 scenario maps show a more uniform seismic ground-motion hazard across Shelby County than scenario maps with constant site conditions (i.e., NEHRP B/C boundary).

The Public School System of Memphis, Tennessee. Report of a Survey Made under the Direction of the Commissioner of Education. Bulletin, 1919, No. 50. Part 6: Industrial Arts, Home Economics and Gardening

ERIC Educational Resources Information Center

Bureau of Education, Department of the Interior, 1920

1920-01-01

In April, 1919, at the request of the Board of Education of Memphis, Tennessee, the United States Commissioner of Education submitted the conditions on which the Bureau of Education would make a survey of the public school system of that city. This study of the Memphis schools is intended to be a study of policies and practices; not of persons.…

Hydrology of aquifer systems in the Memphis area, Tennessee

USGS Publications Warehouse

Criner, James H.; Sun, P-C. P.; Nyman, Dale J.

1964-01-01

The Memphis area as described in .this report comprises about 1,300 square miles of the Mississippi embayment part of the Gulf Coastal Plain. The area is underlain by as much as 3,000 feet of sediments ranging in age from Cretaceous through Quaternary. In 1960, 150 mgd (million gallons per day) of water was pumped from the principal aquifers. Municipal pumpage accounted for almost half of this amount, and industrial pumpage a little more than half. About 90 percent of the water used in the area is derived from the '500-foot' sand, and most of the remainder is from the ?400-foot' sand; both sands are of Eocene age. A small amount of water for domestic use is pumped from the terrace deposits of Pliocene and Pleistocene age. Both the '500-foot' and the '1,400-foot' sands are artesian aquifers except in the southeastern part of the area; there the water level in wells in the '500-foot' sand is now below the overlying confining clay. Water levels in both aquifers have declined almost continuously since pumping began, but the rate of decline has increased rapidly since 1940. Water-level decline in the '1,400-foot' sand has been less pronounced since 1956. The cones of depression in both aquifers have expanded and deepened as a result of the annual increases in pumping, and an increase in hydraulic gradients has induced a greater flow of water into the area. Approximately 135 mgd entered the Memphis area through the '500-foot' sand aquifer in 1960, and, of this amount, 60 mgd originated as inflow from the east and about 75 mgd was derived from leakage from the terrace deposits, from the north, south, and west and from other sources. Of the water entering the '1,400-foot' sand, about 5 mgd was inflow from the east, and about half that amount was from each of the north, south, and west directions. The average rate of movement of water outside the area of heavy withdrawals is about 70 feet per year in the '500-foot' sand and about 40 feet per year in the '1,400-foot' sand

75 FR 72952 - Safety Zone; 1000-yard radius from position 29°48.77′ N 091°33.02′ W, Charenton Drainage and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-29

...-AA00 Safety Zone; 1000-yard radius from position 29[deg]48.77' N 091[deg]33.02' W, Charenton Drainage... position 29[deg]48.77' N 091[deg]33.02' W, Charenton Drainage and Navigation Canal, St. Mary Parish, LA... associated hazards in position 29[deg]48.77' N 091[deg]33.02' W, in the Charenton Drainage and Navigation...

Experienced Based Career Education. Hamilton High School, Memphis, Tennessee. Final Evaluation Report, September 1976, to September 12, 1979.

ERIC Educational Resources Information Center

Miller, Melvin D.; Wilkins, Sandra

A third-party evaluation was designed to document the processes undertaken to implement a Memphis, Tennessee, experience-based career education (EBCE) program. It also intended to assess project effects on student outcomes. Evaluation included pre- and post-testing of a control group and experimental group of tenth grade students enrolled in the…

THE MEMPHIS STORY-MOBILIZING A LARGE SCHOOL SYSTEM AND A COMMUNITY FOR THE SUPERIOR AND TALENTED STUDENT PROJECT.

ERIC Educational Resources Information Center

COUNCE, D. SHELBY

THE STORY OF THE SUPERIOR AND TALENTED STUDENT PROJECT AS IT WAS UTILIZED IN THE MEMPHIS, TENNESSEE, SCHOOL SYSTEM IS RELATED. THE STS PROJECT IS DESIGNED TO ASSIST SECONDARY SCHOOLS IN IDENTIFYING SUPERIOR AND TALENTED STUDENTS, IN PROVIDING GUIDANCE SERVICES FOR THEM, IN ENCOURAGING THEM TO MAKE APPROPRIATE EDUCATIONAL AND OCCUPATIONAL CHOICES,…

78 FR 72004 - Amendment of Class E Airspace; Tazewell, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-02

... Tazewell, TN, as new Standard Instrument Approach Procedures have been developed at New Tazewell Municipal Airport. This enhances the safety and management of aircraft operations at the airport. DATES: Effective..., TN. Airspace reconfiguration is necessary due to the development of the RNAV (GPS) RWY 7 approach and...

PD-1 suppresses development of humoral responses that protect against Tn-bearing tumors

PubMed Central

Haro, Marcela A.; Littrell, Chad A.; Yin, Zhaojun; Huang, Xuefei; Haas, Karen M.

2017-01-01

Tn is a carbohydrate antigen uniquely exposed on tumor mucins and thus, an ideal target for immunotherapy. However, it has been difficult to elicit protective antibody responses against Tn antigen and other tumor associated carbohydrate antigens. Our study demonstrates this can be attributed to PD-1 immuno-inhibition. Our data show a major role for PD-1 in suppressing mucin- and Tn-specific B-cell activation, expansion, and antibody production important for protection against Tn-bearing tumor cells. These Tn/mucin-specific B cells belong to the innate-like B-1b cell subset typically responsible for T cell–independent antibody responses. Interestingly, PD-1–mediated regulation is B cell–intrinsic and CD4+ cells play a key role in supporting Tn/mucin-specific B cell antibody production in the context of PD-1 deficiency. Mucin-reactive antibodies produced in the absence of PD-1 inhibition largely belong to the IgM subclass and elicit potent antitumor effects via a complement-dependent mechanism. The identification of this role for PD-1 in regulating B cell–dependent antitumor immunity to Tn antigen highlights an opportunity to develop new therapeutic strategies targeting tumor associated carbohydrate antigens. PMID:27856425

Potentiometric surface and specific conductance of the Sparta and Memphis aquifers in eastern Arkansas, 1995

USGS Publications Warehouse

Stanton, Gregory P.

1997-01-01

The Sparta and Memphis aquifers in eastern and south-central Arkansas are a major source of water for industrial, public supply, and agricultural uses. An estimated 240 million gallons per day was withdrawn from the Sparta and Memphis aquifers in 1995, an increase of about 17 million gallons per day from 1990. During the spring and early summer of 1995, the water level in the Sparta and Memphis aquifers was measured in 145 wells, the specific conductance of 101 ground-water samples collected from those aquifers was measured. Maps of areal distribution of potentiometric surface and specific conductance generated from these data reveal spatial trends in these parameters across the eastern and south-central Arkansas study area. The altitude of the potentiometric surface ranged from about 206 feet below sea level in Union County to about 307 feet above sea level in Saline County. The potentiometric surface of the Sparta and Memphis aquifers contains cones of depression descending below sea level in the central and southern portions of the study area, and a potentiometric high along the western study area boundary. Major recharge areas exhibit potentiometric highs greater than 200 feet above sea level and specific conductance values less than 200 microsiemens per centimeter, and generally are located in the outcrop/subcrop areas on the southern one-third of the western boundary and the northern portion of the study area. The regional direction of ground-water flow is from the north and west to the south and east, away from the outcrop and subcrop and northern regions, except near areas affected by intense ground-water withdrawals; such areas are manifested by large cones of depression centered in Columbia, Jefferson, and Union Counties. The cones of depression in adjoining Columbia and Union Counties are coalescing at or near sea level. The lowest water level measured was about 206 feet below sea level in Union County. Increased specific conductance values were measured

Characterization of Tn6238 with a New Allele of aac(6′)-Ib-cr

PubMed Central

Quiroga, María P.; Orman, Betina; Errecalde, Laura; Kaufman, Sara

2015-01-01

Here, we report that the genetic structure of Tn1331 remained conserved in Argentina from 1989 to 2013 (72 of 73 isolates), with the exception being the plasmid-borne Tn1331-like transposon Tn6238 containing a new aac(6′)-Ib-cr allele recovered from a colistin-resistant Klebsiella pneumoniae clinical isolate. A bioinformatic analysis of aac(6′)-Ib-like gene cassettes suggests that this new aac(6′)-Ib-cr allele emerged through mutation or homologous recombination in the Tn1331 genetic platform. Tn6238 is a novel platform for the dissemination of aminoglycoside and fluoroquinolone resistance determinants. PMID:25691640

Registration of TN09-008 soybean cyst nematode resistant cultivar

USDA-ARS?s Scientific Manuscript database

The conventional soybean line TN09-008 (Reg. No. CV- , PI ) was released by University of Tennessee Agricultural Research in 2017 as a cultivar, based on high seed yield potential in Tennessee and the southern region. Soybean cultivar TN09-008 is resistant to HG types 1.2.5.7, 5.7, a...

Myofilament Calcium Sensitivity: Mechanistic Insight into TnI Ser-23/24 and Ser-150 Phosphorylation Integration

PubMed Central

Salhi, Hussam E.; Hassel, Nathan C.; Siddiqui, Jalal K.; Brundage, Elizabeth A.; Ziolo, Mark T.; Janssen, Paul M. L.; Davis, Jonathan P.; Biesiadecki, Brandon J.

2016-01-01

Troponin I (TnI) is a major regulator of cardiac muscle contraction and relaxation. During physiological and pathological stress, TnI is differentially phosphorylated at multiple residues through different signaling pathways to match cardiac function to demand. The combination of these TnI phosphorylations can exhibit an expected or unexpected functional integration, whereby the function of two phosphorylations are different than that predicted from the combined function of each individual phosphorylation alone. We have shown that TnI Ser-23/24 and Ser-150 phosphorylation exhibit functional integration and are simultaneously increased in response to cardiac stress. In the current study, we investigated the functional integration of TnI Ser-23/24 and Ser-150 to alter cardiac contraction. We hypothesized that Ser-23/24 and Ser-150 phosphorylation each utilize distinct molecular mechanisms to alter the TnI binding affinity within the thin filament. Mathematical modeling predicts that Ser-23/24 and Ser-150 phosphorylation affect different TnI affinities within the thin filament to distinctly alter the Ca2+-binding properties of troponin. Protein binding experiments validate this assertion by demonstrating pseudo-phosphorylated Ser-150 decreases the affinity of isolated TnI for actin, whereas Ser-23/24 pseudo-phosphorylation is not different from unphosphorylated. Thus, our data supports that TnI Ser-23/24 affects TnI-TnC binding, while Ser-150 phosphorylation alters TnI-actin binding. By measuring force development in troponin-exchanged skinned myocytes, we demonstrate that the Ca2+ sensitivity of force is directly related to the amount of phosphate present on TnI. Furthermore, we demonstrate that Ser-150 pseudo-phosphorylation blunts Ser-23/24-mediated decreased Ca2+-sensitive force development whether on the same or different TnI molecule. Therefore, TnI phosphorylations can integrate across troponins along the myofilament. These data demonstrate that TnI Ser-23

Hydrogeologic framework and simulation of ground-water flow and travel time in the shallow aquifer system in the area of Naval Support Activity Memphis, Millington, Tennessee

USGS Publications Warehouse

Robinson, James L.; Carmichael, John K.; Halford, Keith J.; Ladd, David E.

1997-01-01

Naval Support Activity (NSA) Memphis is a Department of the Navy facility located at the City of Millington, Tennessee, about 5 miles north of Memphis. Contaminants have been detected in surface-water, sediment, and ground-water samples collected at the facility. As part of the Installation Restoration Program, the Navy is considering remedial-action options to prevent or lessen the effect of ground-water contamination at the facility and to control the movement and discharge of contaminants. A numerical model of the ground-water-flow system in the area of NSA Memphis was constructed and calibrated so that quantifiable estimates could be made of ground-water-flow rates, direction, and time-of-travel. The sediments beneath NSA Memphis, to a depth of about 200 feet, form a shallow aquifer system. From youngest to oldest, the stratigraphic units that form the shallow aquifer system are alluvium, loess, fluvial deposits, and the Cockfield and Cook Mountain Formations. The shallow aquifer system is organized into five hydrogeologic units: (1) a confining unit composed of the relatively low permeability sediments of the upper alluvium and the loess; (2) the A1 aquifer comprising sand and gravel of the lower alluvium and the fluvial deposits, and sand lenses in the upper part of the preserved section of the Cockfield Formation; (3) a confining unit composed of clay and silt within the upper part of the Cockfield Formation; (4) the Cockfield aquifer comprising sand lenses within the lower part of the preserved section of the Cockfield Formation; and (5) a confining unit formed by low permeability sediments of the Cook Mountain Formation that composes the upper confining unit for the Memphis aquifer. Thicknesses of individual units vary considerably across the facility. Structural and depositional features that affect the occurrence of ground water in the shallow aquifer system include faulting, an erosional scarp, and 'windows' in the confining units. Underlying the

Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903.

PubMed Central

Grindley, N D; Joyce, C M

1980-01-01

The kanamycin resistance transposon Tn903 consists of a unique region of about 1000 base pairs bounded by a pair of 1050-base-pair inverted repeat sequences. Each repeat contains two Pvu II endonuclease cleavage sites separated by 520 base pairs. We have constructed derivatives of Tn903 in which this 520-base-pair fragment is deleted from one or both repeats. Those derivatives that lack both 520-base-pair fragments cannot transpose, whereas those that lack just one remain transposition proficient. One such transposable derivative, Tn903 delta I, has been selected for further study. We have determined the sequence of the intact inverted repeat. The 18 base pairs at each end are identical and inverted relative to one another, a structure characteristic of insertion sequences. Additional experiments indicate that a single inverted repeat from Tn903 can, in fact, transpose; we propose that this element be called IS903. To correlate the DNA sequence with genetic activities, we have created mutations by inserting a 10-base-pair DNA fragment at several sites within the intact repeat of Tn903 delta 1, and we have examined the effect of such insertions on transposability. The results suggest that IS903 encodes a 307-amino-acid polypeptide (a "transposase") that is absolutely required for transposition of IS903 or Tn903. Images PMID:6261245

75 FR 20774 - Establishment of Class E Airspace; Mountain City, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-21

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 71 [Docket No. FAA-2009-0061; Airspace Docket No. 09-ASO-10] Establishment of Class E Airspace; Mountain City, TN AGENCY... December 7, 2009 that establishes Class E airspace at Johnson County Airport, Mountain City, TN. DATES...

Water levels and water quality in the Sparta-Memphis aquifer (middle Claiborne aquifer) in Arkansas, spring-summer 2011

USGS Publications Warehouse

Schrader, T.P.

2014-01-01

The U.S. Geological Survey, in cooperation with the Arkansas Natural Resources Commission and the Arkansas Geological Survey, has monitored water levels in the Sparta Sand of Claiborne Group and Memphis Sand of Claiborne Group (herein referred to as “the Sparta Sand” and “the Memphis Sand,” respectively) since the 1920s. Groundwater withdrawals have increased while water levels have declined since monitoring was initiated. Herein, aquifers in the Sparta Sand and Memphis Sand will be referred to as “the Sparta-Memphis aquifer” throughout Arkansas. During the spring of 2011, 291 water levels were measured in wells completed in the Sparta-Memphis aquifer and used to produce a regional potentiometric-surface map. During the summer of 2011, groundwater-quality samples were collected and measured from 61 wells for specific conductance, pH, and temperature.In the northern half of Arkansas, the regional direction of groundwater flow in the Sparta-Memphis aquifer is generally to the south-southeast and flows east and south in the southern half of Arkansas. The groundwater in the southern half of Arkansas flows away from the outcrop area except where affected by large depressions in the potentiometric surface. The highest and lowest water-level altitudes measured in the Sparta-Memphis aquifer were 326 feet above and 120 feet below National Geodetic Vertical Datum of 1929 (NGVD 29), respectively.Five depressions are located in the following counties: Arkansas, Cleveland, Jefferson, Lincoln, and Prairie; Union; Cross, Poinsett, St. Francis, and Woodruff; Columbia; and Bradley. Two large depressions, centered in Jefferson and Union Counties, are the result of large withdrawals for industrial, irrigation, or public supply. The depression centered in Jefferson County has expanded in recent years into Arkansas and Prairie Counties as a result of large withdrawals for irrigation and public supply. The lowest water-level altitude measured in this depression is

Removal of carbon, nitrogen and phosphorus from the separated liquid phase of hog manure by the multi-zone BioCAST technology.

PubMed

Yerushalmi, Laleh; Alimahmoodi, Mahmood; Afroze, Niema; Godbout, Stephane; Mulligan, Catherine N

2013-06-15

The removal of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) at concentrations of 960 ± 38 to 2400 ± 96 mg/L, 143 ± 9 to 235 ± 15 mg/L and 25 ± 2 to 57 ± 4 mg/L, respectively, from the separated liquid phase of hog manure by the multi-zone BioCAST technology is discussed. Despite the inhibitory effect of hog waste toward microbial activities, removal efficiencies up to 89.2% for COD, 69.2% for TN and 47.6% for TP were obtained during 185 d of continuous operation. The free ammonia inhibition was postulated to be responsible for the steady reduction of COD and TP removal with the increase of TN/TP ratio from 3.6 to 5.8. On the contrary, the increase of COD/TN ratio from 4.8 to 14.1 improved the removal of all contaminants. Nitrogen removal did not show any dependence on the COD/TP ratio, despite the steady increase of COD and TP removal with this ratio in the range of 19.3-50.6. The removal efficiencies of organic and inorganic contaminants increased progressively owing to the adaptation of microbial biomass, resulting from the presence of suspended biomass in the mixed liquor that circulated continuously between the three zones of aerobic, microaerophilic and anoxic, as well as the attached biomass immobilized inside the aerobic zone. Copyright © 2013 Elsevier B.V. All rights reserved.

The diagnostic value of two commercially available human cTnI assays in goat kids with myocarditis.

PubMed

Karapinar, Tolga; Eroksuz, Yesari; Hayirli, Armagan; Beytut, Enver; Kaynar, Ozgur; Baydar, Ersoy; Sozdutmaz, Ibrahim; Isidan, Hakan

2016-03-01

Cardiac troponin I (cTnI) is a peripheral blood marker for myocardial damage. Because of the unavailability of goat-specific cTnI assays human cTnI assays may be validated for detection of myocarditis in goat kids. The purpose of the study was to evaluate 2 commercially available human cTnI assays in goat kids with myocardial damage, and to determine the cTnI expression in cardiac muscle. Plasma cTnI concentrations were measured in healthy goat kids (n = 7) and goat kids with myocardial damage (n = 8) using the Beckman Coulter Access Accu TnI and the Biomérieux Vidas Ultra. The results were correlated with gross necropsy and histopathologic findings, and cTnI immunhistochemistry in cardiac tissue. Macro- and microscopic findings confirmed myocardial damage in the myocarditis group. Mean plasma cTnI concentration was significantly higher in the myocarditis group than in the healthy control group (104.82 vs 0.02 ng/mL). The overall mean plasma cTnI concentration measured by Biomérieux Vidas Ultra (61.75 ng/mL, 95% CI: 19.55-103.95) was comparable to the mean measured by Beckman Coulter Access Accu TnI (50.08 ng/mL, 95% CI: 24.11-76.06), and cTnI concentrations measured by these assays were highly correlated (r = .977) with a -6.2% bias. Both assays were precise and accurate. The human-specific Beckman Coulter Access Accu TnI and the Biomérieux Vidas Ultra can be used for diagnostic confirmation of myocardial damage in caprine medicine. © 2016 American Society for Veterinary Clinical Pathology.

Transposon mutagenesis of Xylella fastidiosa by electroporation of Tn5 synaptic complexes.

PubMed

Guilhabert, M R; Hoffman, L M; Mills, D A; Kirkpatrick, B C

2001-06-01

Pierce's disease, a lethal disease of grapevine, is caused by Xylella fastidiosa, a gram-negative, xylem-limited bacterium that is transmitted from plant to plant by xylem-feeding insects. Strains of X. fastidiosa also have been associated with diseases that cause tremendous losses in many other economically important plants, including citrus. Although the complete genome sequence of X. fastidiosa has recently been determined, the inability to transform or produce transposon mutants of X. fastidiosa has been a major impediment to understanding pathogen-, plant-, and insect-vector interactions. We evaluated the ability of four different suicide vectors carrying either Tn5 or Tn10 transposons as well as a preformed Tn5 transposase-transposon synaptic complex (transposome) to transpose X. fastidiosa. The four suicide vectors failed to produce any detectable transposition events. Electroporation of transposomes, however, yielded 6 x 10(3) and 4 x 10(3) Tn5 mutants per microg of DNA in two different grapevine strains of X. fastidiosa. Molecular analysis showed that the transposition insertions were single, independent, stable events. Sequence analysis of the Tn5 insertion sites indicated that the transpositions occur randomly in the X. fastidiosa genome. Transposome-mediated mutagenesis should facilitate the identification of X. fastidiosa genes that mediate plant pathogenicity and insect transmission.

EnviroAtlas - Memphis, TN - Estimated Percent Tree Cover Along Walkable Roads

EPA Pesticide Factsheets

This EnviroAtlas dataset estimates tree cover along walkable roads. The road width is estimated for each road and percent tree cover is calculated in a 8.5 meter strip beginning at the estimated road edge. Percent tree cover is calculated for each block between road intersections. Tree cover provides valuable benefits to neighborhood residents and walkers by providing shade, improved aesthetics, and outdoor gathering spaces. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

EnviroAtlas - Memphis, TN - Tree Cover Configuration and Connectivity, Water Background

EPA Pesticide Factsheets

This EnviroAtlas dataset categorizes forest land cover into structural elements (e.g. core, edge, connector, etc.). Forest is defined as Trees & Forest and Woody Wetlands. Water was considered background (value 129) during the analysis to create this dataset, however it has been converted into value 10 to distinguish it from land area background. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as downloadable data (https://edg.epa.gov/data/Public/ORD/EnviroAtlas) or as an EnviroAtlas map service. Additional descriptive information about each attribute in this dataset can be found in its associated EnviroAtlas Fact Sheet (https://www.epa.gov/enviroatlas/enviroatlas-fact-sheets).

RadNet Air Data From Nashville, TN

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Nashville, TN from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

RadNet Air Data From Knoxville, TN

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Knoxville, TN from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

A new step aeration approach towards the improvement of nitrogen removal in a full scale Carrousel oxidation ditch.

PubMed

Jin, Pengkang; Wang, Xianbao; Wang, Xiaochang; Ngo, Huu Hao; Jin, Xin

2015-12-01

Two aeration modes, step aeration and point aeration, were used in a full-scale Carrousel oxidation ditch with microporous aeration. The nitrogen removal performance and mechanism were analyzed. With the same total aeration input, both aeration modes demonstrated good nitrification outcomes with the average efficiency in removing NH4(+)-N of more than 98%. However, the average removal efficiencies for total nitrogen were 89.3% and 77.6% under step aeration and point aeration, respectively. The results indicated that an extended aerobic zone followed the aeration zones could affect the proportion of anoxic and oxic zones. The step aeration with larger anoxic zones indicated better TN removal efficiency. More importantly, step aeration provided the suitable environment for both nitrifiers and denitrifiers. The diversity and relative abundance of denitrifying bacteria under the step aeration (1.55%) was higher than that under the point aeration (1.12%), which resulted in an overall higher TN removal efficiency. Copyright © 2015 Elsevier Ltd. All rights reserved.

78 FR 27029 - Modification of Class C Airspace; Nashville International Airport; TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-09

...-0031; Airspace Docket No. 12-AWA-7] Modification of Class C Airspace; Nashville International Airport... modifies the Nashville International Airport, TN, Class C airspace area by removing a cutout from the... modify the Nashville International Airport, TN, Class C airspace area (78 FR 6257). Interested parties...

cTnT can be a useful marker for early detection of anthracycline cardiotoxicity.

PubMed

Kilickap, S; Barista, I; Akgul, E; Aytemir, K; Aksoyek, S; Aksoy, S; Celik, I; Kes, S; Tekuzman, G

2005-05-01

The level of serum cardiac troponin-T (cTnT) increases with myocardial damage. We sought to assess whether cTnT level could be a useful marker for the early detection of anthracycline cardiotoxicity. Forty-one patients who had been scheduled to receive anthracycline-containing combination chemotherapy were included in the study. Serum cTnT levels were measured before (baseline) and after the first cycle of chemotherapy, and again, after the last cycle of chemotherapy. In all patients, the left ventricular ejection fraction (LVEF), fractional shortening (FS), early peak flow/atrial flow velocity (E/A) ratio, and the isovolemic relaxation time (IRT) were measured echocardiographically, both before and after the completion of chemotherapy. LVEF and FS did not change in any patients. In 21 patients (49%), the E/A ratio decreased after therapy as compared to the pre-treatment values. The decrease in E/A ratio was more prominent in patients who were older than the mean age of our study group, which was 44 years. The post-treatment IRT was prolonged compared with the pretreatment IRT (94.0 +/- 2.0 versus 85.6 +/- 10.5 ms, respectively). cTnT levels after completion of therapy were elevated in 14 (34%) patients, and exceeded the upper limit of the normal range (>0.1 ng/ml) in only one patient. cTnT levels measured after completion of therapy were significantly higher, compared with those measured at baseline and after the first cycle of therapy. In the younger age group (< or =44 years old), there was a two-fold decrease in the E/A ratio in those patients whose cTnT levels increased during the therapy, when compared with those whose cTnT levels did not change (21% versus 43%, respectively). Increased serum cTnT level can be detected in the early stages of anthracycline therapy and it is associated with diastolic dysfunction of the left ventricle. Therefore, serum cTnT level could be a useful measure for early detection of anthracycline-induced cardiotoxicity.

Iceland's Grímsvötn volcano erupts

NASA Astrophysics Data System (ADS)

Showstack, Randy

2011-05-01

About 13 months after Iceland's Eyjafjallajökull volcano began erupting on 14 April 2010, which led to extensive air traffic closures over Europe, Grímsvötn volcano in southeastern took its turn. Iceland's most active volcano, which last erupted in 2004 and lies largely beneath the Vatnajökull ice cap, began its eruption activity on 21 May, with the ash plume initially reaching about 20 kilometers in altitude, according to the Icelandic Meteorological Office. Volcanic ash from Grímsvötn has cancelled hundreds of airplane flights and prompted U.S. president Barack Obama to cut short his visit to Ireland. As Eos went to press, activity at the volcano was beginning to subside.

Iceland: Grímsvötn Volcano

Atmospheric Science Data Center

2013-04-17

article title:  Grímsvötn Volcano Injects Ash into the Stratosphere     ... p.m. local time (1730 UTC) on Saturday, May 21, 2011. The volcano, located approximately 140 miles (220 kilometers) east of the capital ...

Bed-Sediment Sampling and Analysis for Physical and Chemical Properties of the Lower Mississippi River near Memphis, Tennessee

USGS Publications Warehouse

Blanchard, Robert A.; Wagner, Daniel M.; Evans, Dennis A.

2010-01-01

In February 2010, the U.S. Geological Survey, in cooperation with the U.S. Army Corps of Engineers, Memphis District, investigated the presence of inorganic elements and organic compounds in bed sediments of the lower Mississippi River. Selected sites were located in the navigation channel near river miles 737, 773, and 790 near Memphis, Tennessee. Bed-sediment samples were collected using a Shipek grab sampler mounted to a boom crane with a motorized winch. Samples then were processed and shipped to the U.S. Geological Survey Sediment Laboratory in Rolla, Missouri, the USGS National Water Quality Laboratory in Denver, Colorado, and to TestAmerica Laboratory, Inc. in West Sacramento, California. Samples were analyzed for grain size, inorganic elements (including mercury), and organic compounds. Chemical results were tabulated and listed with sediment-quality guidelines and presented with the physical property results. All of the bed material samples collected during this investigation yielded concentrations that were less than the Consensus-Based Probable Effect Concentration guidelines. The physical properties were tabulated and listed using a standard U.S. Geological Survey scale of sizes by class for sediment analysis. All of the samples collected during this investigation indicated a percent composition mostly comprised of sand, ranging from less than 0.125 millimeters to less than 2 millimeters.

The novel conjugative transposon tn1207.3 carries the macrolide efflux gene mef(A) in Streptococcus pyogenes.

PubMed

Santagati, Maria; Iannelli, Francesco; Cascone, Carmela; Campanile, Floriana; Oggioni, Marco R; Stefani, Stefania; Pozzi, Gianni

2003-01-01

The macrolide efflux gene mef(A) of the Streptococcus pyogenes clinical strain 2812A was found to be carried by a 52-kb chromosomal genetic element that could be transferred by conjugation to the chromosome of other streptococcal species. The characteristics of this genetic element are typical of conjugative transposons and was named Tn1207.3. The size of Tn1207.3 was established by pulsed-field gel electrophoresis (PFGE), and DNA sequencing analysis showed that the 7,244 bp at the left end of Tn1207.3 were identical to those of the pneumococcal Tn1207.1 element. Tn1207.3-like genetic elements were found to be inserted at a single specific chromosomal site in 12 different clinical isolates S. pyogenes exhibiting the M phenotype of resistance to macrolides and carrying the mef(A) gene. Tn1207.3 was transferred from S. pyogenes 2812A to Streptococcus pneumoniae, and sequence analysis carried out on six independent transconjugants showed that insertion of Tn1207.3 in the pneumococcal genome always occurred at a single specific site as in Tn1207.1. Using MF2, a representative S. pneumoniae transconjugant, as a donor, Tn1207.3 was transferred again by conjugation to S. pyogenes and Streptococcus gordonii. The previously described nonconjugative element Tn1207.1 of S. pneumoniae appears to be a defective element, part of a longer conjugative transposon that carries mef(A) and is found in clinical isolates of S. pyogenes.

The Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD

PubMed Central

Choi, Ki Young; Spencer, Jeanelle M.; Craig, Nancy L.

2014-01-01

The excision of transposon Tn7 from a donor site and its insertion into its preferred target site, attachment site attTn7, is mediated by four Tn7-encoded transposition proteins: TnsA, TnsB, TnsC, and TnsD. Transposition requires the assembly of a nucleoprotein complex containing all four Tns proteins and the DNA substrates, the donor site containing Tn7, and the preferred target site attTn7. TnsA and TnsB together form the heteromeric Tn7 transposase, and TnsD is a target-selecting protein that binds specifically to attTn7. TnsC is the key regulator of transposition, interacting with both the TnsAB transposase and TnsD-attTn7. We show here that TnsC interacts directly with TnsB, and identify the specific region of TnsC involved in the TnsB–TnsC interaction during transposition. We also show that a TnsC mutant defective in interaction with TnsB is defective for Tn7 transposition both in vitro and in vivo. Tn7 displays cis-acting target immunity, which blocks Tn7 insertion into a target DNA that already contains Tn7. We provide evidence that the direct TnsB–TnsC interaction that we have identified also mediates cis-acting Tn7 target immunity. We also show that TnsC interacts directly with the target selector protein TnsD. PMID:24982178

Geologic structures related to New Madrid earthquakes near Memphis, Tennessee, based on gravity and magnetic interpretations

USGS Publications Warehouse

Hildenbrand, T.G.; Stuart, W.D.; Talwani, P.

2001-01-01

New inversions of gravity and magnetic data in the region north of memphis. Tennessee, and south of latitude 36?? define boundaries of regional structures and igneous complexes in the upper crust. Microseismicity patterns near interpreted boundaries suggest that igneous complexes influence the locations of microseismicity. A weak seismicity cluster occurs near one intrusion (Covington pluton), at the intersection of the southwest margin of the Missouri batholith and the southeast margin of the Reelfoot rift. A narrow seismicity trend along the Reelfoot rift axis becomes diffuse near a second intrusion (Osceola intrusive complex) and changes direction to an area along the northwest flank of the intrusion. The axial seismicity trend also contains a tight cluster of earthquakes located just outside the Osceola intrusive complex. The mechanical explanation of the two seismicity patterns is uncertain, but the first cluster may be caused by stress concentration due to the high elastic stiffness and strength of the Covington intrusion. The spatially changing seismicity pattern near the Osceola complex may be caused by the preceding factors plus interaction with faulting along the rift axis. The axial seismicity strand itself is one of several connected and interacting active strands that may produce stress concentrations at strand ends and junctions. The microseismicity clusters at the peripheries of the two intrusions lead us to conclude that these stress concentrations or stressed volumes may be locations of future moderate to large earthquakes near Memphis. Published by Elsevier Science B.V.

Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 Purification and Tagmentation Protocol

PubMed Central

Hennig, Bianca P.; Velten, Lars; Racke, Ines; Tu, Chelsea Szu; Thoms, Matthias; Rybin, Vladimir; Besir, Hüseyin; Remans, Kim; Steinmetz, Lars M.

2017-01-01

Efficient preparation of high-quality sequencing libraries that well represent the biological sample is a key step for using next-generation sequencing in research. Tn5 enables fast, robust, and highly efficient processing of limited input material while scaling to the parallel processing of hundreds of samples. Here, we present a robust Tn5 transposase purification strategy based on an N-terminal His6-Sumo3 tag. We demonstrate that libraries prepared with our in-house Tn5 are of the same quality as those processed with a commercially available kit (Nextera XT), while they dramatically reduce the cost of large-scale experiments. We introduce improved purification strategies for two versions of the Tn5 enzyme. The first version carries the previously reported point mutations E54K and L372P, and stably produces libraries of constant fragment size distribution, even if the Tn5-to-input molecule ratio varies. The second Tn5 construct carries an additional point mutation (R27S) in the DNA-binding domain. This construct allows for adjustment of the fragment size distribution based on enzyme concentration during tagmentation, a feature that opens new opportunities for use of Tn5 in customized experimental designs. We demonstrate the versatility of our Tn5 enzymes in different experimental settings, including a novel single-cell polyadenylation site mapping protocol as well as ultralow input DNA sequencing. PMID:29118030

Brookside Mills, Knox County, TN

EPA Pesticide Factsheets

Brookside Mills, located in Knox County, TN, was a textile mill that was founded in 1885 and at its peak employed over 1,000 people. Its former uses included fabric weaving, dying, and sewing operations. It was at some point a department store, and during a portion of its history, coal was used as an energy source. Weaving operations continued in some form at the Brookside factory until 1969. In 1996 the buildings were demolished.

76 FR 18288 - Tennessee Disaster #TN-00048

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-01

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12497 and 12498] Tennessee Disaster TN-00048 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an... this disaster for physical damage is 12497 6 and for economic injury is 12498 0. The State which...

78 FR 48762 - Tennessee Disaster #TN-00076

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-09

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13696 and 13697] Tennessee Disaster TN-00076 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 08/02/2013. Incident: Severe Storms and...

75 FR 55833 - Tennessee Disaster #TN-00042

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-14

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12303 and 12304] Tennessee Disaster TN-00042 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 09/07/2010. Incident: Severe Storms and...

77 FR 24515 - Notice of Lodging of Consent Decree Pursuant to the Clean Water Act

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-24

... America and the State of Tennessee v. the City of Memphis (``City''), Civil Action No. 2:10-CV- 02083-SHM... Decree represents a settlement of claims against the City of Memphis under [[Page 24516

76 FR 20433 - Tennessee Disaster #TN-00048

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-12

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12497 and 12498] Tennessee Disaster TN-00048 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is a an amendment of the Administrative declaration of a disaster for the State of Tennessee dated 03/23/2011. Incident: Severe Storms and...

Methods to Analyze Bone Regenerative Response to Different rhBMP-2 Doses in Rabbit Craniofacial Defects

DTIC Science & Technology

2014-02-28

sockets.6 The commercially available INFUSE system (Medtronic Spinal and Biologics, Memphis, TN), compris- ing an absorbable collagen sponge plus...a collagen sponge carrier) by Medtronics27 for bone healing in rabbits. Even the 25mg rhBMP-2 dose used showed significantly greater re- generated...visualization No 3D morphological analysis for small-animal modelsCan be repeated over course of healing for temporal trends Potential risk of X-ray

Genetic Networks Activated by Blast Injury to the Eye

DTIC Science & Technology

2013-08-01

SUPPLEMENTARY NOTES 14. ABSTRACT Purpose: The present research project is designed to define the overall change in gene expression in the eye...Memphis TN, 38163 REPORT DATE: August 2013 TYPE OF REPORT: Annual Summary PREPARED FOR: U.S. Army Medical Research and Materiel Command...author(s) and should not be construed as an official Department of the Army position, policy or decision unless so designated by other documentation

Tailoring the Electromagnetic Near Field with Patterned Surfaces: Near-Field Plates

DTIC Science & Technology

2014-12-10

single layer processing. 3 Near-field plates can be viewed as metamaterial surfaces ( metasurfaces or metafilms) [20]. They are textured/structured at a...Applied Physics Letters, vol. 103, 041104, July 2013. 3. C. Pfeiffer and A. Grbic, ”Cascaded metasurfaces for complete phase and polarization control...Antennas and Propagation, pp. 1-2, Memphis, TN, July 6-12, 2014. 5. Pfeiffer and A. Grbic, ”Analysis and synthesis of bianisotropic metasurfaces

Two-stage soil infiltration treatment system for treating ammonium wastewaters of low COD/TN ratios.

PubMed

Lei, Zhongfang; Wu, Ting; Zhang, Yi; Liu, Xiang; Wan, Chunli; Lee, Duu-Jong; Tay, Joo-Hwa

2013-01-01

Soil infiltration treatment (SIT) is ineffective to treat ammonium wastewaters of total nitrogen (TN) > 100 mg l(-1). This study applied a novel two-stage SIT process for effective TN removal from wastewaters of TN>100 mg l(-1) and of chemical oxygen demand (COD)/TN ratio of 3.2-8.6. The wastewater was first fed into the soil column (stage 1) at hydraulic loading rate (HLR) of 0.06 m(3) m(-2) d(-1) for COD removal and total phosphorus (TP) immobilization. Then the effluent from stage 1 was fed individually into four soil columns (stage 2) at 0.02 m(3) m(-2) d(-1) of HLR with different proportions of raw wastewater as additional carbon source. Over the one-year field test, balanced nitrification and denitrification in the two-stage SIT revealed excellent TN removal (>90%) from the tested wastewaters. Copyright © 2012 Elsevier Ltd. All rights reserved.

Wolf River at Memphis, Tennessee: floodflow characteristics along proposed Interstate Highway 240, Shelby County

USGS Publications Warehouse

Randolph, William J.; Gamble, Charles R.

1973-01-01

This report has been prepared by the U.S. Geological Survey at the request of Mr. Henry Derthick, Engineer of Structures of the Tennessee Department of Transportation, under the authority of a cooperative agreement between the two agencies. It supplements information contained in a report with the same title dated September 1966. The Department of Transportation proposes to construct a segment of Interstate Highway 240 and several bridges across the Wolf River on the northern side off Memphis, Shelby Count. Mt. Derthick has requested an analysis of the 50-year flood or the maximum flood of record to determine the possible effect of the proposed construction on flood profiles along the Wolf River.

77 FR 6007 - Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, Drawbridge Operation...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-07

...] Quarterly Listings; Safety Zones, Security Zones, Special Local Regulations, Drawbridge Operation... they could be published in the Federal Register. This notice lists temporary safety zones, security zones, special local regulations, drawbridge operation regulations and regulated navigation areas, all...

Development of GIS Database for New Madrid Seismic Zone

NASA Astrophysics Data System (ADS)

Birhanemeskel, Y. T.; Vlahovic, G.; Arroucau, P.; Malhotra, R.; Powell, C. A.

2010-12-01

The New Madrid Seismic Zone (NMSZ) of the central Mississippi river valley is currently the most seismically active region in the central and eastern United States. A number of earthquakes occurred in NMSZ between 1811 and 1812, of which three major earthquakes with magnitudes greater than 7 destroyed the town of New Madrid, Missouri. Intraplate seismicity like the New Madrid seismicity is difficult to explain in the framework of plate tectonics and requires analyzing various geological, geophysical and seismological data to better understand its causes. ArcGIS® 9.3.1 software with license type ArcEditor was used to build a geodatabase containing multiple layers that are useful for the study of intraplate seismicity. These layers include earthquake locations, gravity and magnetic anomalies, lithology, topography, velocity anomalies as resolved by arrival time tomography and geological structures like intrusions and faults. The data for these layers were obtained from the U.S Geological Survey, from the Center for Earthquake Research and Information at the University of Memphis, TN, and from paper maps. Zipped files of various formats (.xls, .shp, .txt, .tar, etc) were downloaded and converted to a format compatible with ArcGIS. To keep compatibility of the data, editing of the attribute table of the raw data was completed before importing the data to Arc Catalog. Geo-referencing and digitizing processes were also done to import layers of contour lines and geological structures with correct vector information from papers maps. Layers were clipped in order to make sure that they fit the spatial extent of the study area (from 34°S to 40°N in latitude and from 93°W to 86°W in longitude). The New Madrid seismicity will be analyzed by looking for possible relationships that exist between the data layers using various spatial and geostatistical tools. For example the distribution of earthquakes will be analyzed with respect to the potential field and velocity

An Enterobacter plasmid as a new genetic background for the transposon Tn1331

PubMed Central

Alavi, Mohammad R; Antonic, Vlado; Ravizee, Adrien; Weina, Peter J; Izadjoo, Mina; Stojadinovic, Alexander

2011-01-01

Background Genus Enterobacter includes important opportunistic nosocomial pathogens that could infect complex wounds. The presence of antibiotic resistance genes in these microorganisms represents a challenging clinical problem in the treatment of these wounds. In the authors’ screening of antibiotic-resistant bacteria from complex wounds, an Enterobacter species was isolated that harbors antibiotic-resistant plasmids conferring resistance to Escherichia coli. The aim of this study was to identify the resistance genes carried by one of these plasmids. Methods The plasmids from the Enterobacter isolate were propagated in E. coli and one of the plasmids, designated as pR23, was sequenced by the Sanger method using fluorescent dyeterminator chemistry on a genetic analyzer. The assembled sequence was annotated by search of the GenBank database. Results Plasmid pR23 is composed of the transposon Tn1331 and a backbone plasmid that is identical to the plasmid pPIGDM1 from Enterobacter agglomerans. The multidrug-resistance transposon Tn1331, which confers resistance to aminoglycoside and beta lactam antibiotics, has been previously isolated only from Klebsiella. The Enterobacter plasmid pPIGDM1, which carries a ColE1-like origin of replication and has no apparent selective marker, appears to provide a backbone for propagation of Tn1331 in Enterobacter. The recognition sequence of Tn1331 transposase for insertion into pPIGDM1 is the pentanucleotide TATTA, which occurs only once throughout the length of this plasmid. Conclusion Transposition of Tn1331 into the Enterobacter plasmid pPIGDM1 enables this transposon to propagate in this Enterobacter. Since Tn1331 was previously isolated only from Klebsiella, this report suggests horizontal transfer of this transposon between the two bacterial genera. PMID:22259249

Effects of HCM cTnI Mutation R145G on Troponin Structure and Modulation by PKA Phosphorylation Elucidated by Molecular Dynamics Simulations

PubMed Central

Lindert, Steffen; Cheng, Yuanhua; Kekenes-Huskey, Peter; Regnier, Michael; McCammon, J. Andrew

2015-01-01

Cardiac troponin (cTn) is a key molecule in the regulation of human cardiac muscle contraction. The N-terminal cardiac-specific peptide of the inhibitory subunit of troponin, cTnI (cTnI1-39), is a target for phosphorylation by protein kinase A (PKA) during β-adrenergic stimulation. We recently presented evidence indicating that this peptide interacts with the inhibitory peptide (cTnl137–147) when S23 and S24 are phosphorylated. The inhibitory peptide is also the target of the point mutation cTnI-R145G, which is associated with hypertrophic cardiomyopathy (HCM), a disease associated with sudden death in apparently healthy young adults. It has been shown that both phosphorylation and this mutation alter the cTnC-cTnI (C-I) interaction, which plays a crucial role in modulating contractile activation. However, little is known about the molecular-level events underlying this modulation. Here, we computationally investigated the effects of the cTnI-R145G mutation on the dynamics of cTn, cTnC Ca2+ handling, and the C-I interaction. Comparisons were made with the cTnI-R145G/S23D/S24D phosphomimic mutation, which has been used both experimentally and computationally to study the cTnI N-terminal specific effects of PKA phosphorylation. Additional comparisons between the phosphomimic mutations and the real phosphorylations were made. For this purpose, we ran triplicate 150 ns molecular dynamics simulations of cTnI-R145G Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, cTnI-R145G/S23D/S24D Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, and cTnI-R145G/PS23/PS24 Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, respectively. We found that the cTnI-R145G mutation did not impact the overall dynamics of cTn, but stabilized crucial Ca2+-coordinating interactions. However, the phosphomimic mutations increased overall cTn fluctuations and destabilized Ca2+ coordination. Interestingly, cTnI-R145G blunted the intrasubunit interactions between the cTnI N-terminal extension and the cTnI inhibitory

Effects of HCM cTnI mutation R145G on troponin structure and modulation by PKA phosphorylation elucidated by molecular dynamics simulations.

PubMed

Lindert, Steffen; Cheng, Yuanhua; Kekenes-Huskey, Peter; Regnier, Michael; McCammon, J Andrew

2015-01-20

Cardiac troponin (cTn) is a key molecule in the regulation of human cardiac muscle contraction. The N-terminal cardiac-specific peptide of the inhibitory subunit of troponin, cTnI (cTnI(1-39)), is a target for phosphorylation by protein kinase A (PKA) during β-adrenergic stimulation. We recently presented evidence indicating that this peptide interacts with the inhibitory peptide (cTnl(137-147)) when S23 and S24 are phosphorylated. The inhibitory peptide is also the target of the point mutation cTnI-R145G, which is associated with hypertrophic cardiomyopathy (HCM), a disease associated with sudden death in apparently healthy young adults. It has been shown that both phosphorylation and this mutation alter the cTnC-cTnI (C-I) interaction, which plays a crucial role in modulating contractile activation. However, little is known about the molecular-level events underlying this modulation. Here, we computationally investigated the effects of the cTnI-R145G mutation on the dynamics of cTn, cTnC Ca(2+) handling, and the C-I interaction. Comparisons were made with the cTnI-R145G/S23D/S24D phosphomimic mutation, which has been used both experimentally and computationally to study the cTnI N-terminal specific effects of PKA phosphorylation. Additional comparisons between the phosphomimic mutations and the real phosphorylations were made. For this purpose, we ran triplicate 150 ns molecular dynamics simulations of cTnI-R145G Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), cTnI-R145G/S23D/S24D Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), and cTnI-R145G/PS23/PS24 Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), respectively. We found that the cTnI-R145G mutation did not impact the overall dynamics of cTn, but stabilized crucial Ca(2+)-coordinating interactions. However, the phosphomimic mutations increased overall cTn fluctuations and destabilized Ca(2+) coordination. Interestingly, cTnI-R145G blunted the intrasubunit interactions between the cTnI N

76 FR 36166 - Tennessee Disaster Number TN-00051

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-21

... U.S. SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12556 and 12557] Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4. SUMMARY: This is an... loan applications to: U.S. Small Business Administration, Processing and Disbursement Center, 14925...

76 FR 48939 - Tennessee Disaster Number TN-00058

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-09

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12704 and 12705] Tennessee Disaster Number TN-00058 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of... information in the original declaration remains unchanged. (Catalog of Federal Domestic Assistance Numbers...

75 FR 35103 - Tennessee Disaster Number TN-00038

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-21

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12161 and 12162] Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 8. SUMMARY: This is an amendment of the... the original declaration remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and...

75 FR 35103 - Tennessee Disaster Number TN-00039

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-21

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12159 and 12160] Tennessee Disaster Number TN-00039 AGENCY: Small Business Administration. ACTION: Amendment 7. SUMMARY: This is an amendment of the... Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster...

Court Documents Related to Martin Luther King, Jr., and Memphis Sanitation Workers. The Constitution Community: Postwar United States (1945 to Early 1970s).

ERIC Educational Resources Information Center

Perry, Douglas

During inclement weather in Memphis, Tennessee in February 1968, two separate incidents caused black sanitation workers to strike for job safety, better wages and benefits, and union recognition. Mayor Henry Loeb was unsympathetic and opposed to the union. Martin Luther King agreed to lend his support to the sanitation workers and spoke at a rally…

Time constraints on the origin of large volume basalts derived from O-isotope and trace element mineral zoning and U-series disequilibria in the Laki and Grímsvötn volcanic system

NASA Astrophysics Data System (ADS)

Bindeman, Ilya N.; Sigmarsson, Olgeir; Eiler, John

2006-05-01

The 1783-1784 AD fissure eruption of Laki (Iceland) produced 15 km 3 of homogeneous basaltic lavas and tephra that are characterized by extreme (3‰) 18O-depletion relative to normal mantle. Basaltic tephra erupted over the last 8 centuries and as late as in November 2004 from the Grímsvötn central volcano, which together with Laki are a part of a single volcanic system, is indistinguishable in δ18O from Laki glass. This suggests that all tap a homogeneous and long-lived low- δ18O magma reservoir. In contrast, we observe extreme oxygen isotope heterogeneity (2.2-5.2‰) in olivine and plagioclase contained within these lavas and tephra, and disequilibrium mineral-glass oxygen-isotope fractionations. Such low- δ18O glass values, and extreme 3‰ range in δ18O olivine have not been described in any other unaltered basalt. The energy constrained mass balance calculation involving oxygen isotopes and major element composition calls for an origin of the Laki-Grímsvötn quartz tholeiitic basaltic melts with δ18O = 3.1‰ by bulk digestion of low- δ18O hydrated basaltic crust with δ18O = - 4‰ to + 1‰, rather than magma mixing with ultra-low- δ18O silicic melt. The abundant Pleistocene hyaloclastites, which were altered by synglacial meltwaters, can serve as a likely assimilant material for the Grímsvötn magmas. The ( 226Ra / 230Th) activity ratio in Laki lavas and 20th century Grímsvötn tephras is 13% in-excess of secular equilibrium, but products of the 20th century Grímsvötn eruptions have equilibrium ( 210Pb / 226Ra). Modeling of oxygen isotope exchange between disequilibrium phenocrysts and magmas, and these short-lived U-series nuclides yields a coherent age for the Laki-Grímsvötn magma reservoir between 100 and 1000 yrs. We propose the existence of uniquely fingerprinted, low- δ18O, homogeneous, large volume, and long-lived basaltic reservoir beneath the Laki-Grímsvötn volcanic system that has been kept alive in its position above the

Multicenter Evaluation of a 0-Hour/1-Hour Algorithm in the Diagnosis of Myocardial Infarction With High-Sensitivity Cardiac Troponin T.

PubMed

Mueller, Christian; Giannitsis, Evangelos; Christ, Michael; Ordóñez-Llanos, Jorge; deFilippi, Christopher; McCord, James; Body, Richard; Panteghini, Mauro; Jernberg, Tomas; Plebani, Mario; Verschuren, Franck; French, John; Christenson, Robert; Weiser, Silvia; Bendig, Garnet; Dilba, Peter; Lindahl, Bertil

2016-07-01

We aim to prospectively validate the diagnostic accuracy of the recently developed 0-h/1-h algorithm, using high-sensitivity cardiac troponin T (hs-cTnT) for the early rule-out and rule-in of acute myocardial infarction. We enrolled patients presenting with suspected acute myocardial infarction and recent (<6 hours) onset of symptoms to the emergency department in a global multicenter diagnostic study. Hs-cTnT (Roche Diagnostics) and sensitive cardiac troponin I (Siemens Healthcare) were measured at presentation and after 1 hour, 2 hours, and 4 to 14 hours in a central laboratory. Patient triage according to the predefined hs-cTnT 0-hour/1-hour algorithm (hs-cTnT below 12 ng/L and Δ1 hour below 3 ng/L to rule out; hs-cTnT at least 52 ng/L or Δ1 hour at least 5 ng/L to rule in; remaining patients to the "observational zone") was compared against a centrally adjudicated final diagnosis by 2 independent cardiologists (reference standard). The final diagnosis was based on all available information, including coronary angiography and echocardiography results, follow-up data, and serial measurements of sensitive cardiac troponin I, whereas adjudicators remained blinded to hs-cTnT. Among 1,282 patients enrolled, acute myocardial infarction was the final diagnosis for 213 (16.6%) patients. Applying the hs-cTnT 0-hour/1-hour algorithm, 813 (63.4%) patients were classified as rule out, 184 (14.4%) were classified as rule in, and 285 (22.2%) were triaged to the observational zone. This resulted in a negative predictive value and sensitivity for acute myocardial infarction of 99.1% (95% confidence interval [CI] 98.2% to 99.7%) and 96.7% (95% CI 93.4% to 98.7%) in the rule-out zone (7 patients with false-negative results), a positive predictive value and specificity for acute myocardial infarction of 77.2% (95% CI 70.4% to 83.0%) and 96.1% (95% CI 94.7% to 97.2%) in the rule-in zone, and a prevalence of acute myocardial infarction of 22.5% in the observational zone. The hs-cTn

Learning when to Hold'em and When to Fold'em: ERS's Budget Hold'em Game Facilitates the Budget Development Process in Memphis

ERIC Educational Resources Information Center

Education Resource Strategies, 2010

2010-01-01

If your school district is facing a budget issue, it might surprise you to learn that the solution might very well lie in a game of cards. That certainly was the case earlier this year for the city schools of Memphis, Tennessee. The game is called Budget Hold'em, and it was developed by Education Resource Strategies (ERS) of Watertown,…

76 FR 14855 - Television Broadcasting Services; Nashville, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-18

... FEDERAL COMMUNICATIONS COMMISSION 47 CFR Part 73 [MB Docket No. 11-29, RM-11622; DA 11-335] Television Broadcasting Services; Nashville, TN AGENCY: Federal Communications Commission. ACTION: Proposed... reply comments on or before May 2, 2011. ADDRESSES: Federal Communications Commission, Office of the...

76 FR 27739 - Tennessee Disaster Number TN-00051

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-12

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12556 and 12557] Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of... Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance. [FR Doc...

76 FR 32387 - Tennessee Disaster Number TN-00053

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-06

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12572 and 12573] Tennessee Disaster Number TN-00053 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of... unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate...

Geographic Profiling: Knowledge Through Prediction

DTIC Science & Technology

2014-06-01

FTO Foreign Terrorist Organization GIS geographic information systems LE lawless elements MADM multi-attribute decision making MILF Moro Islamic...or her life. Anchor points can include the offender’s home , his or her workplace, a home of a friend of the offender, or even a bar or restaurant...more generally for the improvement of police patrols. In Memphis, TN, city officials have seen a decrease in crime with the help of operation Blue

Role of Myofibril-Inducing RNA in cardiac TnT expression in developing Mexican axolotl

PubMed Central

Sferrazza, Gian-Franco; Zhang, Chi; Jia, Pingping; Lemanski, Sharon L.; Athauda, Gagani; Stassi, Alyssa; Halager, Kristine; Maier, Jennifer A.; Rueda-de-Leon, Elena; Gupta, Amit; Dube, Syamalima; Huang, Xupei; Prentice, Howard M.; Dube, Dipak K.; Lemanski, Larry F.

2007-01-01

The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene “c”, for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts.. Myofibril-Inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac Troponin-T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart PMID:17408593

77 FR 50929 - Security Zones; 2012 RNC Bridge Security Zones, Captain of the Port St. Petersburg Zone, Tampa, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-23

...-AA87 Security Zones; 2012 RNC Bridge Security Zones, Captain of the Port St. Petersburg Zone, Tampa, FL... temporary security zones around certain bridges on the waters of Pinellas County and Tampa Bay, Florida..., or mooring on waters within 50 yards of the designated bridges during the times that the security...

Response of high-risk of recurrence/progression bladder tumours expressing sialyl-Tn and sialyl-6-T to BCG immunotherapy

PubMed Central

Lima, L; Severino, P F; Silva, M; Miranda, A; Tavares, A; Pereira, S; Fernandes, E; Cruz, R; Amaro, T; Reis, C A; Dall'Olio, F; Amado, F; Videira, P A; Santos, L; Ferreira, J A

2013-01-01

Background: High risk of recurrence/progression bladder tumours is treated with Bacillus Calmette-Guérin (BCG) immunotherapy after complete resection of the tumour. Approximately 75% of these tumours express the uncommon carbohydrate antigen sialyl-Tn (Tn), a surrogate biomarker of tumour aggressiveness. Such changes in the glycosylation of cell-surface proteins influence tumour microenvironment and immune responses that may modulate treatment outcome and the course of disease. The aim of this work is to determine the efficiency of BCG immunotherapy against tumours expressing sTn and sTn-related antigen sialyl-6-T (s6T). Methods: In a retrospective design, 94 tumours from patients treated with BCG were screened for sTn and s6T expression. In vitro studies were conducted to determine the interaction of BCG with high-grade bladder cancer cell line overexpressing sTn. Results: From the 94 cases evaluated, 36 had recurrence after BCG treatment (38.3%). Treatment outcome was influenced by age over 65 years (HR=2.668; (1.344–5.254); P=0.005), maintenance schedule (HR=0.480; (0.246–0.936); P=0.031) and multifocallity (HR=2.065; (1.033–4.126); P=0.040). sTn or s6T expression was associated with BCG response (P=0.024; P<0.0001) and with increased recurrence-free survival (P=0.001). Multivariate analyses showed that sTn and/or s6T were independent predictive markers of recurrence after BCG immunotherapy (HR=0.296; (0.148–0.594); P=0.001). In vitro studies demonstrated higher adhesion and internalisation of the bacillus to cells expressing sTn, promoting cell death. Conclusion: s6T is described for the first time in bladder tumours. Our data strongly suggest that BCG immunotherapy is efficient against sTn- and s6T-positive tumours. Furthermore, sTn and s6T expression are independent predictive markers of BCG treatment response and may be useful in the identification of patients who could benefit more from this immunotherapy. PMID:24064971

Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells.

PubMed

Muroi, K; Suda, T; Nakamura, M; Okada, S; Nojiri, H; Amemiya, Y; Miura, Y; Hakomori, S

1994-01-01

The epitopes Tn and sialosyl-Tn are expressed on erythrocytes of individuals with a very rare blood group, who often suffer from "Tn syndrome." We surveyed expression of Tn and sialosyl-Tn in normal blood cells, malignant transformed cells, and progenitor stem cells from bone marrow (BM). An anti-Tn antibody, IE3, and an anti-sialosyl-Tn antibody, TKH2, were used in this study. TKH2 reacted with erythroblasts, B cells, and a subset of CD4+ cells; but not with erythrocytes. Erythroblastic cell lines (K562, HEL, and UT7/EPO) and B-cell lines (Daudi, Raji, and B-cell lines transformed by Epstein-Barr virus) showed reactivity to TKH2. Similar results from the reactivity of TKH2 with transformed cells from leukemia patients and lymphoma patients were obtained; TKH2 reacted with blasts from erythroleukemia (M6; for 4 of 4 cases) and with lymphocytes from B-cell chronic lymphocytic leukemia (3 of 3), B-cell lymphoma (5 of 5), and CD4+ adult T-cell leukemia (4 of 4), but did not react with blasts from acute myeloid leukemia (M0 to M5; 0 of 22) or acute lymphoid leukemia (B-lymphoid leukemia, 0 of 11; T-lymphoid leukemia, 0 of 2; undifferentiated leukemia, 0 of 1). IE3 did not react with all of the tested cells. CD2-CD19-TKH2+ normal BM cells (BMC) contained blasts and various maturation stages of erythroblasts. The TKH2+ cells produced a large number of colony-forming unit-erythroid (CFU-E) colonies, whereas they produced a small number of burst-forming unit-erythroid colonies and CFU-granulocyte-macrophage colonies. CD34+ normal BMC did not express Tn and sialosyl-Tn. These findings suggest that sialosyl-Tn expresses in CFU-E to erythroblasts.

77 FR 59551 - Safety Zone; Fireworks Event in Captain of the Port New York Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-28

... Zone; Fireworks Event in Captain of the Port New York Zone AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast Guard will enforce a safety zone in the Captain of the Port New York Zone on the specified date and time. This action is necessary to ensure the safety of vessels and...

76 FR 23524 - Safety Zone, Brandon Road Lock and Dam to Lake Michigan Including Des Plaines River, Chicago...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-27

...-AA00 Safety Zone, Brandon Road Lock and Dam to Lake Michigan Including Des Plaines River, Chicago... safety zone from Brandon Road Lock and Dam to Lake Michigan. This proposed safety zone will cover 77.... This TIR established a 77 mile long safety zone from Brandon Road Lock to Lake Michigan in Chicago, IL...

Julian Davies and the discovery of kanamycin resistance transposon Tn5.

PubMed

Berg, Douglas E

2017-04-01

This paper recounts some of my fond memories of a collaboration between Julian Davies and myself that started in 1974 in Geneva and that led to our serendipitous discovery of the bacterial kanamycin resistance transposon Tn5, and aspects of the lasting positive impact of our interaction and discovery on me and the community. Tn5 was one of the first antibiotic resistance transposons to be found. Its analysis over the ensuing decades provided valuable insights into mechanisms and control of transposition, and led to its use as a much-valued tool in diverse areas of molecular genetics, as also will be discussed here.

76 FR 4147 - Putnam-Cumberland, TN-Improve Power Supply

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

... TENNESSEE VALLEY AUTHORITY Putnam-Cumberland, TN--Improve Power Supply AGENCY: Tennessee Valley... proposed electrical power supply improvements in the Putnam and Cumberland region of east-central Tennessee... supplies bulk electric power to Cumberland and Putnam counties and the immediately surrounding areas in...

Structural Analysis of the Hg(II)-Regulatory Protein Tn501 MerR from Pseudomonas aeruginosa

NASA Astrophysics Data System (ADS)

Wang, Dan; Huang, Shanqing; Liu, Pingying; Liu, Xichun; He, Yafeng; Chen, Weizhong; Hu, Qingyuan; Wei, Tianbiao; Gan, Jianhua; Ma, Jing; Chen, Hao

2016-09-01

The metalloprotein MerR is a mercury(II)-dependent transcriptional repressor-activator that responds to mercury(II) with extraordinary sensitivity and selectivity. It’s widely distributed in both Gram-negative and Gram-positive bacteria but with barely detectable sequence identities between the two sources. To provide structural basis for the considerable biochemical and biophysical experiments previously performed on Tn501 and Tn21 MerR from Gram-negative bacteria, we analyzed the crystal structure of mercury(II)-bound Tn501 MerR. The structure in the metal-binding domain provides Tn501 MerR with a high affinity for mercury(II) and the ability to distinguish mercury(II) from other metals with its unique planar trigonal coordination geometry, which is adopted by both Gram-negative and Gram-positive bacteria. The mercury(II) coordination state in the C-terminal metal-binding domain is transmitted through the allosteric network across the dimer interface to the N-terminal DNA-binding domain. Together with the previous mutagenesis analyses, the present data indicate that the residues in the allosteric pathway have a central role in maintaining the functions of Tn501 MerR. In addition, the complex structure exhibits significant differences in tertiary and quaternary structural arrangements compared to those of Bacillus MerR from Gram-positive bacteria, which probably enable them to function with specific promoter DNA with different spacers between -35 and -10 elements.

Late Pleistocene and Holocene paleoseismology of an intraplate seismic zone in a large alluvial valley, the New Madrid seismic zone, Central USA

NASA Astrophysics Data System (ADS)

Guccione, Margaret J.

2005-10-01

The New Madrid seismic zone (NMSZ) is an intraplate right-lateral strike-slip and thrust fault system contained mostly within the Mississippi Alluvial Valley. The most recent earthquake sequence in the zone occurred in 1811 1812 and had estimated moment magnitudes of 7 8 (e.g., [Johnston, A.C., 1996. Seismic moment assessment of stable continental earthquakes, Part 3: 1811 1812 New Madrid, 1886 Charleston, and 1755 Lisbon. Geophysical Journal International 126, 314 344; Johnston, A.C., Schweig III, E.S, 1996. The enigma of the New Madrid earthquakes of 1811 1812. Annual Reviews of Earth and Planetary Sciences 24, 339 384; Hough, S.E., Armbruster, J.G., Seeber, L., Hough, J.F., 2000. On the modified Mercalli intensities and magnitudes of the New Madrid earthquakes. Journal of Geophysical Research 105 (B10), 23,839 23,864; Tuttle, M.P., 2001. The use of liquefaction features in paleoseismology: Lessons learned in the New Madrid seismic zone, central United States. Journal of Seismology 5, 361 380]). Four earlier prehistoric earthquakes or earthquake sequences have been dated A.D. 1450 ± 150, 900 ± 100, 300 ± 200, and 2350 B.C. ± 200 years using paleoliquefaction features, particularly those associated with native American artifacts, and in some cases surface deformation ([Craven, J. A. 1995. Paleoseismology study in the New Madrid seismic zone using geological and archeological features to constrain ages of liquefaction deposits. M.S thesis, University of Memphis, Memphis, TN, U.S.A.; Tuttle, M.P., Lafferty III, R.H., Guccione, M.J., Schweig III, E.S., Lopinot, N., Cande, R., Dyer-Williams, K., Haynes, M., 1996. Use of archaeology to date liquefaction features and seismic events in the New Madrid seismic zone, central United States. Geoarchaeology 11, 451 480; Guccione, M.J., Mueller, K., Champion, J., Shepherd, S., Odhiambo, B., 2002b. Stream response to repeated co-seismic folding, Tiptonville dome, western Tennessee. Geomorphology 43(2002), 313 349; Tuttle, M

76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-20

... Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the National... Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park Service... services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not to exceed 3...

76 FR 1512 - Amendment of Class E Airspace; Savannah, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-11

..., Georgia 30320; telephone (404) 305-5610. SUPPLEMENTARY INFORMATION: History On October 26, 2010, the FAA... areas extending upward from 700 feet or more above the surface of the earth. * * * * * ASO TN E5...

Posted wait times an added advantage to multi-facility systems?

PubMed

2011-04-01

Methodist Le Bonheur Healthcare in Memphis, TN, is investigating whether posting ED wait times via the internet can positively impact patient flow in the six EDs the health system operates in the Memphis region. The health system began posting wait times in August 2010, resulting in increases in ED volume ranging from 6% to 10%. The health system is monitoring ED arrivals by zip code to assess any impact on load balancing between its busy EDs. One marketing challenge is that a competitor is posting ED wait times as well, but it is posting the time it takes for a patient to be placed in a bed as opposed to the door-to-provider time that Methodist Le Bonheur is posting. The approach has the most impact on lower-acuity patients, but experts worry that in the future, payers may not be reimbursed for ED care for these patients.

African American mothers' self-described discipline strategies with young children in 1992 and 2012.

PubMed

LeCuyer, Elizabeth A; Christensen, Julie J; Kreher, Donna; Kearney, Margaret H; Kitzman, Harriet J

2015-01-01

In this qualitative descriptive study, 30 young, unmarried, low-income African American mothers in Memphis, TN, were interviewed in 2011-2012 about their discipline strategies with their 12- to 19-month-old children. Using content analyses, their strategies were described and compared with those from a similar sample in 1992. Findings suggest both continuity and change during that 20-year period. More mothers in 2011-2012 described the use of distraction and time out, suggesting a wider variety of strategies than were used in 1992. These findings may help clinicians to better understand disciplinary methods in young low-income African American mothers such as these in Memphis. Approaching mothers in a respectful and culturally sensitive manner will help them focus on effective, developmentally appropriate strategies consistent with their own parenting goals. Copyright © 2015 National Association of Pediatric Nurse Practitioners. Published by Elsevier Inc. All rights reserved.

78 FR 55770 - [Tennessee Disaster greek-iTN-00077

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-11

... SMALL BUSINESS ADMINISTRATION Disaster Declaration 13737 and 13738 [Tennessee Disaster TN-00077] AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of TENNESSEE dated 08/26/2013. Incident: Severe Storms and...

A critical evaluation of the Beckman Coulter Access hsTnI: Analytical performance, reference interval and concordance.

PubMed

Pretorius, Carel J; Tate, Jillian R; Wilgen, Urs; Cullen, Louise; Ungerer, Jacobus P J

2018-05-01

We investigated the analytical performance, outlier rate, carryover and reference interval of the Beckman Coulter Access hsTnI in detail and compared it with historical and other commercial assays. We compared the imprecision, detection capability, analytical sensitivity, outlier rate and carryover against two previous Access AccuTnI assay versions. We established the reference interval with stored samples from a previous study and compared the concordances and variances with the Access AccuTnI+3 as well as with two commercial assays. The Access hsTnI had excellent analytical sensitivity with the calibration slope 5.6 times steeper than the Access AccuTnI+3. The detection capability was markedly improved with the SD of the blank 0.18-0.20 ng/L, LoB 0.29-0.33 ng/L and LoD 0.58-0.69 ng/L. All the reference interval samples had a result above the LoB value. At a mean concentration of 2.83 ng/L the SD was 0.28 ng/L (CV 9.8%). Carryover (0.005%) and outlier (0.046%) rates were similar to the Access AccuTnI+3. The combined male and female 99th percentile reference interval was 18.2 ng/L (90% CI 13.2-21.1 ng/L). Concordance amongst the assays was poor with only 16.7%, 19.6% and 15.2% of samples identified by all 4 assays as above the 99th, 97.5th and 95th percentiles. Analytical imprecision was a minor contributor to the observed variances between assays. The Beckman Coulter Access hsTnI assay has excellent analytical sensitivity and precision characteristics close to zero. This allows cTnI measurement in all healthy individuals and the capability to identify numerically small differences between serial samples as statistically significant. Concordance in healthy individuals remains poor amongst assays. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

High-sensitive cardiac troponin hs-TnT levels in sudden deaths related to atherosclerotic coronary artery disease.

PubMed

Beausire, Tim; Faouzi, Mohamed; Palmiere, Cristian; Fracasso, Tony; Michaud, Katarzyna

2018-06-04

Ischemic heart disease (IHD) related to atherosclerotic coronary artery disease (CAD) is one of the most prevalent causes of death in Europe. Postmortem evaluation of IHD remains a challenge because of possible non-specific autopsy finding in some autopsy cases, especially in early myocardial ischemia. High-sensitive cardiac troponin T (hs-TnT) is used today in clinical practice as the "gold standard" to diagnose the myocardial ischemia, and might also be applied as an ancillary tool for post-mortem evaluation. The goal of this study is to evaluate the diagnostic value of post-mortem serum hs-TnT assay in cases of sudden death related to IHD. We will also investigate the influence of cardiopulmonary resuscitation (CPR) attempts on post-mortem hs-TnT levels. The hs-TnT values in serum were retrospectively analysed in 85 autopsy data. 52 cases with clinical history and morphological results suggesting cardiac ischemia were included in the study group (mean age 53.5; age range 34-75) and 33 cases in the control group (mean age 40.4; age range 15-69). The group's statistical comparison was performed using logistic regression model. Our study showed a significant non-linear association between hs-TnT serum values and post-mortem diagnosis of sudden deaths related to IHD (p-value 0.005). The shape of the relationship is showing that the probability of death due to IHD increases quickly with a light level of hs-TnT (maximum around 90ng/L) then decreases slightly while remaining at high in values. No significant difference in the hs-TnT serum values was found between the CPR and the non-CPR cases (p-value 0.304). The measurement of hs-TnT serum values might be considered as an ancillary tool for the evaluation of death related to IHD, while taking necessary precautions in the interpretation of the results. Copyright © 2018 Elsevier B.V. All rights reserved.

77 FR 19970 - Security Zones; 2012 Republican National Convention, Captain of the Port St. Petersburg Zone...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-03

...-AA87 Security Zones; 2012 Republican National Convention, Captain of the Port St. Petersburg Zone... Marine Science Technician First Class Nolan L. Ammons, Sector St. Petersburg Prevention Department, Coast... proposed rule would establish seven temporary security zones in the Captain of the Port St. Petersburg Zone...

Experimental manipulation of TN:TP ratiossuppress cyanobacterial biovolume and microcystinconcentration in large-scale in situ mesocosms

USGS Publications Warehouse

Harris, Theodore D.; Wilhelm, Frank M.; Graham, Jennifer L.; Loftin, Keith A.

2014-01-01

A global dataset was compiled to examine relations between the total nitrogen to total phosphorus ratio (TN:TP) and microcystin concentration in lakes and reservoirs. Microcystin concentration decreased as TN:TP ratios increased, suggesting that manipulation of the TN:TP ratio may reduce microcystin concentrations. This relationship was experimentally tested by adding ammonium nitrate to increase the TN:TP ratio in large-scale (70 m3), in situ mesocosms located in a eutrophic reservoir that routinely experiences toxic blooms of cyanobacteria. At a TN:TP ratio >75:1, chlorophytes dominated the phytoplankton community in the mesocosms, while cyanobacterial biovolume was significantly reduced and microcystin was not detected. In contrast, the unmanipulated reservoir was dominated by cyanobacteria, and microcystin was detected. Secchi depths were 1.1 to 1.8 times greater in the mesocosms relative to the reservoir. Cladoceran zooplankton had a larger body size (0.14 mm on average) in the mesocosms compared to conspecifics in the reservoir, which was likely related to the higher quality food. Combined, these empirical and experimental data indicate that although nutrient addition is counterintuitive to current cyanobacteria management practices, increasing the TN:TP ratio by adding nitrogen may be a potential short-term management strategy to reduce cyanobacteria and cyanotoxins when other alternatives (e.g., phosphorus reduction) are not possible. Additional experimental studies with careful controls are needed to define best management practices and identify any potential unintended consequences before nitrogen addition is implemented as a lake and reservoir management practice.

77 FR 30245 - Safety Zones; Annual Fireworks Events in the Captain of the Port Detroit Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-22

...-AA00 Safety Zones; Annual Fireworks Events in the Captain of the Port Detroit Zone AGENCY: Coast Guard... by adding three permanent safety zones within the Captain of the Port Detroit Zone. This action is necessary to provide for the safety of life and property on navigable waters during each event. This action...

77 FR 75559 - Safety Zone; Fireworks Event in Captain of the Port New York Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-21

... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket No. USCG-2012-1064] Safety Zone; Fireworks Event in Captain of the Port New York Zone AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast Guard will enforce a safety zone in the Captain of the Port New...

Structural Analysis of the Hg(II)-Regulatory Protein Tn501 MerR from Pseudomonas aeruginosa

PubMed Central

Wang, Dan; Huang, Shanqing; Liu, Pingying; Liu, Xichun; He, Yafeng; Chen, Weizhong; Hu, Qingyuan; Wei, Tianbiao; Gan, Jianhua; Ma, Jing; Chen, Hao

2016-01-01

The metalloprotein MerR is a mercury(II)-dependent transcriptional repressor-activator that responds to mercury(II) with extraordinary sensitivity and selectivity. It’s widely distributed in both Gram-negative and Gram-positive bacteria but with barely detectable sequence identities between the two sources. To provide structural basis for the considerable biochemical and biophysical experiments previously performed on Tn501 and Tn21 MerR from Gram-negative bacteria, we analyzed the crystal structure of mercury(II)-bound Tn501 MerR. The structure in the metal-binding domain provides Tn501 MerR with a high affinity for mercury(II) and the ability to distinguish mercury(II) from other metals with its unique planar trigonal coordination geometry, which is adopted by both Gram-negative and Gram-positive bacteria. The mercury(II) coordination state in the C-terminal metal-binding domain is transmitted through the allosteric network across the dimer interface to the N-terminal DNA-binding domain. Together with the previous mutagenesis analyses, the present data indicate that the residues in the allosteric pathway have a central role in maintaining the functions of Tn501 MerR. In addition, the complex structure exhibits significant differences in tertiary and quaternary structural arrangements compared to those of Bacillus MerR from Gram-positive bacteria, which probably enable them to function with specific promoter DNA with different spacers between −35 and −10 elements. PMID:27641146

78 FR 29020 - Safety Zone; Tennessee River, Mile 463.5 to 464.5; Chattanooga, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-17

... hazards associated with the Riverbend Festival fireworks. Entry into this zone is prohibited unless... protect persons and vessels from potential safety hazards associated with the Riverbend Festival fireworks. The Riverbend Festival fireworks display takes place on the Tennessee River and is launched from the...

77 FR 42176 - Safety Zones; Annual Fireworks Events in the Captain of the Port Detroit Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-18

... fireworks launch site located at position 41-34'-18.10'' N, 082-51'-18.70'' W (NAD 83). This zone will be... fireworks launch site located at position 41-39'- 19'' N, 082-48'-57'' W (NAD 83). This zone will be...'' W (NAD 83). This zone will be enforced one evening during the first week in July. The safety zone...

Transponson Tn916 Mutagenesis in Bacillus anthracis,

DTIC Science & Technology

1987-11-10

Tngla, is described. Tng1a was transferred from Streptococcus 1aJaji strain DS16C1 to f. a VNR-1 by conjugation in a standard filter mating procedure...transposon, Tn916, mutagenesis, Bacillus, anthracis, subtilis. , Streptococcus , faecalis, aro 2.AUSrN ACT (Cautious no reverse efho if nece.at7r sd ideratfy...transferred from Streptococcus : faecalis strain DS16CI to B. anthracis VNR-1 by conjugation in a standard filter mating procedure. Tetracycline

The fully synthetic MAG-Tn3 therapeutic vaccine containing the tetanus toxoid-derived TT830-844 universal epitope provides anti-tumor immunity.

PubMed

Laubreton, Daphné; Bay, Sylvie; Sedlik, Christine; Artaud, Cécile; Ganneau, Christelle; Dériaud, Edith; Viel, Sophie; Puaux, Anne-Laure; Amigorena, Sebastian; Gérard, Catherine; Lo-Man, Richard; Leclerc, Claude

2016-03-01

Malignant transformations are often associated with aberrant glycosylation processes that lead to the expression of new carbohydrate antigens at the surface of tumor cells. Of these carbohydrate antigens, the Tn antigen is particularly highly expressed in many carcinomas, especially in breast carcinoma. We designed MAG-Tn3, a fully synthetic vaccine based on three consecutive Tn moieties that are O-linked to a CD4+ T cell epitope, to induce anti-Tn antibody responses that could be helpful for therapeutic vaccination against cancer. To ensure broad coverage within the human population, the tetanus toxoid-derived peptide TT830-844 was selected as a T-helper epitope because it can bind to various HLA-DRB molecules. We showed that the MAG-Tn3 vaccine, which was formulated with the GSK proprietary immunostimulant AS15 and designed for human cancer therapy, is able to induce an anti-Tn antibody response in mice of various H-2 haplotypes, and this response correlates with the ability to induce a specific T cell response against the TT830-844 peptide. The universality of the TT830-844 peptide was extended to new H-2 and HLA-DRB molecules that were capable of binding this T cell epitope. Finally, the MAG-Tn3 vaccine was able to induce anti-Tn antibody responses in cynomolgus monkeys, which targeted Tn-expressing tumor cells and mediated tumor cell death both in vitro and in vivo. Thus, MAG-Tn3 is a highly promising anticancer vaccine that is currently under evaluation in a phase I clinical trial.

The Dandridge-Vonore Fault Zone in the Eastern Tennessee Seismic Zone (and Rejuvenation of the Smokies?)

NASA Astrophysics Data System (ADS)

Cox, R. T.; Hatcher, R. D., Jr.; Forman, S. L.; Gamble, E. D. S.; Warrell, K. F.

2017-12-01

The eastern Tennessee seismic zone (ETSZ) trends 045o from NE Alabama and NW Georgia through Tennessee to SE Kentucky, and seismicity is localized 5-26 km deep in the basement. The ETSZ is the second most seismically active region in North America east of the Rocky Mountains, although no historic earthquakes larger than Mw 4.8 have been recorded here. Late Quaternary paleoiseismic evidence suggests that the ETSZ is capable of M7+ earthquakes and that neotectonic faults may have significantly influenced the regional relief. We have identified an 80 km-long, 060o-trending corridor in eastern Tennessee that contains collinear northeast-striking thrust, strike-slip, and normal Quaternary faults with displacements of 1-2 m, herein termed the Dandridge-Vonore fault zone (DVFZ). French Broad River alluvium in the northeast DVFZ near Dandridge, TN, is displaced by a 050o-striking, SE-dipping thrust fault and by a set of related fissures that record at least two significant post 25 ka paleo-earthquakes. Southwest of Dandridge near Alcoa, TN, a 060o-striking, SE-dipping thrust fault cuts Little River alluvium and records two significant post-15 ka paleo-earthquakes. Farther southwest at Vonore, colluvium with alluvial cobbles is thrust >1 m by a 057o-striking, steeply SE-dipping fault that may also have a significant strike-slip component, and Little Tennessee River alluvium is dropped >2 m along a 070o- striking normal fault. The DVFZ partly overlaps and is collinear with a local trend of maximum seismicity that extends 30 km farther SW of the DVFZ (as currently mapped), for a total length of 110 km. The DVFZ is coincident with a steep gradient in S-wave velocities (from high velocity on the SE to low velocity on the NW) at mid-crustal depths of 20 to 24 km, consistent with a fault and source zone at hypocentral depths in the crystalline basement. Moreover, the DVFZ parallels the NW foot of Blue Ridge Mountains, and the sense of thrusting at all sites of Quaternary faulting

Earthquake Hazard in the New Madrid Seismic Zone Remains a Concern

USGS Publications Warehouse

Frankel, A.D.; Applegate, D.; Tuttle, M.P.; Williams, R.A.

2009-01-01

There is broad agreement in the scientific community that a continuing concern exists for a major destructive earthquake in the New Madrid seismic zone. Many structures in Memphis, Tenn., St. Louis, Mo., and other communities in the central Mississippi River Valley region are vulnerable and at risk from severe ground shaking. This assessment is based on decades of research on New Madrid earthquakes and related phenomena by dozens of Federal, university, State, and consulting earth scientists. Considerable interest has developed recently from media reports that the New Madrid seismic zone may be shutting down. These reports stem from published research using global positioning system (GPS) instruments with results of geodetic measurements of strain in the Earth's crust. Because of a lack of measurable strain at the surface in some areas of the seismic zone over the past 14 years, arguments have been advanced that there is no buildup of stress at depth within the New Madrid seismic zone and that the zone may no longer pose a significant hazard. As part of the consensus-building process used to develop the national seismic hazard maps, the U.S. Geological Survey (USGS) convened a workshop of experts in 2006 to evaluate the latest findings in earthquake hazards in the Eastern United States. These experts considered the GPS data from New Madrid available at that time that also showed little to no ground movement at the surface. The experts did not find the GPS data to be a convincing reason to lower the assessment of earthquake hazard in the New Madrid region, especially in light of the many other types of data that are used to construct the hazard assessment, several of which are described here.

Impacts of climate change on TN load and its control in a River Basin with complex pollution sources.

PubMed

Yang, Xiaoying; Warren, Rachel; He, Yi; Ye, Jinyin; Li, Qiaoling; Wang, Guoqing

2018-02-15

It is increasingly recognized that climate change could affect the quality of water through complex natural and anthropogenic mechanisms. Previous studies on climate change and water quality have mostly focused on assessing its impact on pollutant loads from agricultural runoff. A sub-daily SWAT model was developed to simulate the discharge, transport, and transformation of nitrogen from all known anthropogenic sources including industries, municipal sewage treatment plants, concentrated and scattered feedlot operations, rural households, and crop production in the Upper Huai River Basin. This is a highly polluted basin with total nitrogen (TN) concentrations frequently exceeding Class V of the Chinese Surface Water Quality Standard (GB3838-2002). Climate change projections produced by 16 Global Circulation Models (GCMs) under the RCP 4.5 and RCP 8.5 scenarios in the mid (2040-2060) and late (2070-2090) century were used to drive the SWAT model to evaluate the impacts of climate change on both the TN loads and the effectiveness of three water pollution control measures (reducing fertilizer use, constructing vegetative filter strips, and improving septic tank performance) in the basin. SWAT simulation results have indicated that climate change is likely to cause an increase in both monthly average and extreme TN loads in February, May, and November. The projected impact of climate change on TN loads in August is more varied between GCMs. In addition, climate change is projected to have a negative impact on the effectiveness of septic tanks in reducing TN loads, while its impacts on the other two measures are more uncertain. Despite the uncertainty, reducing fertilizer use remains the most effective measure for reducing TN loads under different climate change scenarios. Meanwhile, improving septic tank performance is relatively more effective in reducing annual TN loads, while constructing vegetative filter strips is more effective in reducing annual maximum monthly

Toxicity of sediment collected upriver and downriver of major cities along the lower Mississippi River

USGS Publications Warehouse

Winger, P.V.; Lasier, P.J.

1998-01-01

The Lower Mississippi River contributes significantly to the biodiversity and ecological stability of the alluvial valley. Agricultural, industrial and municipal developments have historically impacted environmental quality of the river. Toxicity of sediment and sediment pore water was used to assess the current effects of major cities on sediment quality along the Lower Mississippi River. Composite sediment samples were collected from four sites upriver and four sites downriver of five major cities: Cairo, IL; Memphis, TN; Vicksburg, MS; Baton Rouge, LA; and New Orleans, LA. Following EPA's standard methods for acute toxicity testing of freshwater solid-phase sediment, Hyalella azteca were exposed to the sediments for 10 d with two water renewals per day. Hyalella azteca were also exposed for 96 h to pore water extracted from the sediments. After the initial tests, the animals were exposed to ultraviolet light for 12 h. Sediments were analyzed for organics (organochlorine pesticides, PCBs, organophosphate insecticides, and PAHs) and metals (Cr, Cu, Pb, Mn, Ni, Zn). With the exception of upriver from Memphis, solid-phase sediments were not toxic to H. azteca. Pore water from sediments collected upriver of Memphis showed slight toxicity. Exposure of H. azteca to ultraviolet light did not increase the toxicity of the sediment or pore-water samples, indicating a lack of PAH toxicity. Chemical analyses did not reveal any contaminant levels of concern in the sediments. Based on toxicity testing and chemical analyses, quality of sediments collected from the Lower Mississippi was good, with the exception of sites sampled upriver of Memphis.

Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs).

PubMed

Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

2014-01-01

Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons--Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element "captured" with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not

77 FR 69435 - Grant of Authority for Subzone Status and Partial Approval of Manufacturing Authority; Toho Tenax...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-19

... Polyacrylonitrile Fiber and Carbon Fiber), Rockwood, TN Pursuant to its authority under the Foreign-Trade Zones Act...-purpose subzone at the oxidized polyacrylonitrile fiber (OPF) and carbon fiber manufacturing and... manufacture 24K or higher tow, standard grade carbon fiber for export; and Whereas, at this time, the Board is...

Assessing the impact of dairy waste lagoons on groundwater quality using a spatial analysis of vadose zone and groundwater information in a coastal phreatic aquifer.

PubMed

Baram, S; Kurtzman, D; Ronen, Z; Peeters, A; Dahan, O

2014-01-01

Dairy waste lagoons are considered to be point sources of groundwater contamination by chloride (Cl(-)), different nitrogen-species and pathogens/microorganisms. The objective of this work is to introduce a methodology to assess the past and future impacts of such lagoons on regional groundwater quality. The method is based on a spatial statistical analysis of Cl(-) and total nitrogen (TN) concentration distributions in the saturated and the vadose (unsaturated) zones. The method provides quantitative data on the relation between the locations of dairy lagoons and the spatial variability in Cl(-) and TN concentrations in groundwater. The method was applied to the Beer-Tuvia region, Israel, where intensive dairy farming has been practiced for over 50 years above the local phreatic aquifer. Mass balance calculations accounted for the various groundwater recharge and abstraction sources and sinks in the entire region. The mass balances showed that despite the small surface area covered by the dairy lagoons in this region (0.8%), leachates from lagoons have contributed 6.0% and 12.6% of the total mass of Cl(-) and TN (mainly as NO3(-)-N) added to the aquifer. The chemical composition of the aquifer and vadose zone water suggested that irrigated agricultural activity in the region is the main contributor of Cl(-) and TN to the groundwater. A low spatial correlation between the Cl(-) and NO3(-)-N concentrations in the groundwater and the on-land location of the dairy farms strengthened this assumption, despite the dairy waste lagoon being a point source for groundwater contamination by Cl(-) and NO3(-)-N. Mass balance calculations, for the vadose zone of the entire region, indicated that drying of the lagoons would decrease the regional groundwater salinization process (11% of the total Cl(-) load is stored under lagoons). A more considerable reduction in the groundwater contamination by NO3(-)-N is expected (25% of the NO3(-)-N load is stored under lagoons). Results

77 FR 9528 - Security Zone; Protection of Military Cargo, Captain of the Port Zone Puget Sound

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-17

... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket No. USCG-2012-0087] Security Zone; Protection of Military Cargo, Captain of the Port Zone Puget Sound AGENCY: Coast Guard, DHS... Waterway Security Zone in Commencement Bay, Tacoma, Washington from 6 a.m. on February 17, 2012, through 11...

The effect and biological mechanism of COD/TN ratio on nitrogen removal in a novel upflow microaerobic sludge reactor treating manure-free piggery wastewater.

PubMed

Li, Jianzheng; Meng, Jia; Li, Jiuling; Wang, Cheng; Deng, Kaiwen; Sun, Kai; Buelna, Gerardo

2016-06-01

A novel upflow microaerobic sludge reactor (UMSR) was constructed to treat manure-free piggery wastewater with high NH4(+)-N concentration and low COD/TN ratio, and the effect and biological mechanism of COD/TN ratio on nitrogen removal were investigated at a constant hydraulic retention time of 8h and 35°C. The results showed that the UMSR could treat the wastewater with a better synchronous removal of COD, NH4(+)-N and TN. The microaerobic UMSR allowed nitrifiers, and heterotrophic and autotrophic denitrifiers to thrive in the flocs, revealing a multiple nitrogen removal mechanism in the reactor. Both the nitrifiers and denitrifiers would be restricted by an influent COD/TN ratio more than 0.82, resulting in a decrease of TN removal in the UMSR. To get a TN removal over 80% with a TN load removal above 0.86kg/(m(3)·d) in the UMSR, the influent COD/TN ratio should be less than 0.70. Copyright © 2016 Elsevier Ltd. All rights reserved.

Observed Changes in Atmospheric Boundary Layer Properties at Memphis International Airport During August 1995

NASA Technical Reports Server (NTRS)

Zak, J. Allen; Rodgers, William G., Jr.

1997-01-01

As part of the NASA Terminal Area Productivity Program, Langley Research Center embarked on a series of field measurements of wake vortex characteristics and associated atmospheric boundary layer properties. One measurement period was at the Memphis International Airport in August 1995. Atmospheric temperature, humidity, winds, turbulence, radiation, and soil properties were measured from a variety of sensor systems and platforms including sodars, profilers, aircraft and towers. This research focused on: (1) changes that occurred in tower data during sunrise and sunset transitions, (2) vertical variation of temperature and cross-head winds at selected times utilizing combinations of sensors, and (3) changes measured by an OV-10 aircraft during approaches and level flights. Significant but not unusual changes are documented and discussed in terms of expected boundary layer behavior. Questions on measurement and prediction of these changes from existing and near-term capabilities are discussed in the context of a future Aircraft Vortex Spacing System.

Establishment of an Arbitrary PCR for Rapid Identification of Tn917 Insertion Sites in Staphylococcus epidermidis: Characterization of Biofilm-Negative and Nonmucoid Mutants

PubMed Central

Knobloch, Johannes K.-M.; Nedelmann, Max; Kiel, Kathrin; Bartscht, Katrin; Horstkotte, Matthias A.; Dobinsky, Sabine; Rohde, Holger; Mack, Dietrich

2003-01-01

Transposon mutagenesis with the Enterococcus faecalis transposon Tn917 is a genetic approach frequently used to identify genes related with specific phenotypes in gram-positive bacteria. We established an arbitrary PCR for the rapid and easy identification of Tn917 insertion sites in Staphylococcus epidermidis with six independent, well-characterized biofilm-negative Tn917 transposon mutants, which were clustered in the icaADBC gene locus or harbor Tn917 in the regulatory gene rsbU. For all six of these mutants, short chromosomal DNA fragments flanking both transposon ends could be amplified. All fragments were sufficient to correctly identify the Tn917 insertion sites in the published S. epidermidis genomes. By using this technique, the Tn917 insertion sites of three not-yet-characterized biofilm-negative or nonmucoid mutants were identified. In the biofilm-negative and nonmucoid mutant M12, Tn917 is inserted into a gene homologous to the regulatory gene purR of Bacillus subtilis and Staphylococcus aureus. The Tn917 insertions of the nonmucoid but biofilm-positive mutants M16 and M20 are located in genes homologous to components of the phosphoenolpyruvate-sugar phosphotransferase system (PTS) of B. subtilis, S. aureus, and Staphylococcus carnosus, indicating an influence of the PTS on the mucoid phenotype in S. epidermidis. PMID:14532029

Comparison of the gentamicin resistance transposon Tn5281 with regions encoding gentamicin resistance in Enterococcus faecalis isolates from diverse geographic locations.

PubMed Central

Hodel-Christian, S L; Murray, B E

1992-01-01

The genetic determinant encoding gentamicin resistance (Gmr) on the beta-lactamase encoding plasmid pBEM10 of Enterococcus faecalis HH22 is carried on a transposon, termed Tn5281, that is highly related to the staphylococcal Gmr transposons Tn4001 found in Australian isolates of Staphylococcus aureus and Tn4031 found in United States isolates of Staphylococcus epidermidis. We have now studied plasmid DNA from Gmr strains of E. faecalis isolated from diverse geographical locations (Houston, Pennsylvania, Thailand, and Chile) by using restriction endonuclease analysis and DNA-DNA hybridization to determine whether other Gmr E. faecalis carry Tn5281 or a similar type of element. We also compared these enterococci to several United States isolates of Staphylococcus aureus with nonmobile Gmr determinants. Three E. faecalis isolates (from Houston and Chile) carried Tn5281-like elements, whereas two isolates (from Houston and Pennsylvania) had restriction endonuclease and DNA-DNA hybridization patterns more similar to those of the Tn4001-IS257 hybrid found in the nonmobile Gmr determinants in United States isolates of S. aureus. A strain from Thailand had a third pattern unrelated to either Tn5281 or the nonmobile Gmr determinants present in United States isolates of S. aureus. Our results demonstrate that there is both similarity and diversity between the Gmr determinant of strains of E. faecalis isolated in diverse geographic locations. Images PMID:1332593

77 FR 66072 - Designation of New Grantee; Foreign Trade Zone 66, Wilmington, NC, and Foreign-Trade Zone 67...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-01

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1861] Designation of New Grantee; Foreign Trade Zone 66, Wilmington, NC, and Foreign-Trade Zone 67, Morehead City, NC Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u), and the Foreign-Trade Zones Board Regulations (15 CFR part...

77 FR 24838 - Safety Zone; Magothy River, Sillery Bay, MD

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-26

...-AA00 Safety Zone; Magothy River, Sillery Bay, MD AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The Coast Guard is establishing a safety zone in certain waters of the Magothy River, in Sillery Bay... a notice of proposed rulemaking (NPRM) entitled ``Safety Zone; Magothy River, Sillery Bay, MD'' in...

Optimization of high-rate TN removal in a novel constructed wetland integrated with microelectrolysis system treating high-strength digestate supernatant.

PubMed

Guo, Luchen; He, Keli; Wu, Shubiao; Sun, Hao; Wang, Yanfei; Huang, Xu; Dong, Renjie

2016-08-01

The potential of high-rate TN removal in three aerated horizontal subsurface-flow constructed wetlands to treat high-strength anaerobic digestate supernatant was evaluated. Different strategies of intermittent aeration and effluent recirculation were applied to compare their effect on nitrogen depuration performance. Additional glucose supply and iron-activated carbon based post-treatment systems were established and examined, respectively, to further remove nitrate that accumulated in the effluents from aerated wetlands. The results showed that intermittent aeration (1 h on:1 h off) significantly improved nitrification with ammonium removal efficiency of 90% (18.1 g/(m(2)·d)), but limited TN removal efficiency (53%). Even though effluent recirculation (a ratio of 1:1) increased TN removal from 53% to 71%, the effluent nitrate concentration was still high. Additional glucose was used as a post-treatment option and further increased the TN removal to 82%; however, this implementation caused additional organic pollution. Furthermore, the iron-activated carbon system stimulated with a microelectrolysis process achieved greater than 85% effluent nitrate removal and resulted in 86% TN removal. Considering the high TN removal rate, aerated constructed wetlands integrated with a microelectrolysis-driven system show great potential for treating high-strength digestate supernatant. Copyright © 2016 Elsevier Ltd. All rights reserved.

Enhanced biological nutrients removal using an integrated oxidation ditch with vertical circle from wastewater by adding an anaerobic column.

PubMed

Wang, Shu-mei; Liu, Jun-xin

2005-01-01

Compared to conventional oxidation ditches, an integrated oxidation ditch with vertical circle (IODVC) has the characters of concise configuration, simple operation and maintenance, land saving and automatical sludge returning. By the utilization of vertical circulation, an aerobic zone and an anoxic zone can be unaffectedly formed in the IODVC. Therefore, COD and nitrogen can be efficiently removed. However, the removal efficiency of phosphorus was low in the IODVC. In the experiment described, a laboratory scale system to add an anaerobic column to the IODVC has been tested to investigate the removal of phosphorus from wastewater. The experimental results showed that the removal efficiency of TP with the anaerobic column was increased to 54.0% from 22.3% without the anaerobic column. After the acetic sodium was added into the influent as carbon sources, the mean TP removal efficency of 77.5% was obtained. At the same time, the mean removal efficiencies of COD, TN and NH3-N were 92.2%, 81.6% and 98.1%, respectively, at 12 h of HRT and 21-25 d of SRT. The optimal operational conditions in this study were as follows: recycle rate = 1.5-2.0, COD/TN > 6, COD/TP > 40, COD loading rate = 0.26-0.32 kgCOD/(kgSS x d), TN loading rate = 0.028-0.034 kgTN/(kgSS x d) and TP loading rate = 0.003-0.005 kgTP/(kgSS x d), respectively.

A Low Precipitation Supercell over the Southeast US: A Case Study

NASA Technical Reports Server (NTRS)

Robert, Barry C.; Knupp, Kevin R.; Buechler, Dennis

1998-01-01

On 28 March 1997 a low precipitation supercell storm was observed 125 km east of Memphis, TN. The storm exhibited a visual appearance similar to that of Great Plains low precipitation (LP) supercells while it was being video typed for 35 min. beginning at 2335 UTC. While the storm produced hailstones up to 4.5 cm in diameter, and had a 4 hour lifetime, tornadoes were absent. However, over 20 tornadoes were produced in Kentucky and Tennessee (TN) by other thunderstorms during that afternoon and evening. The purpose of this paper is to document this LP supercell that occurred in the Southeastern US. To the authors' knowledge an LP storm has not yet been documented in the Southeastern US. This paper presents a detailed overview of the LP supercell, including synoptic conditions, radar observations, lightning data and visual observations of the storm.

Comparison of cardiac TnI outliers using a contemporary and a high-sensitivity assay on the Abbott Architect platform.

PubMed

Ryan, J B; Southby, S J; Stuart, L A; Mackay, R; Florkowski, C M; George, P M

2014-07-01

Assays for cardiac troponin (cTn) have undergone improvements in sensitivity and precision in recent years. Increased rates of outliers, however, have been reported on various cTn platforms, typically giving irreproducible, falsely higher results. We aimed to evaluate the outlier rate occurring in patients with elevated cTnI using a contemporary and high-sensitivity assay. All patients with elevated cTnI (up to 300 ng/L) performed over a 21-month period were assayed in duplicate. A contemporary assay (Abbott STAT Troponin-I) was used for the first part of the study and subsequently a high-sensitivity assay (Abbott STAT High-Sensitive Troponin-I) was used. Outliers exceeded a calculated critical difference (CD) (CD = z × √2 × SDAnalytical) where z = 3.5 (for probability of 0.0005) and critical outliers also were on a different side of the decision level. The respective outlier and critical outlier rates were 0.22% and 0.10% for the contemporary assay (n = 4009) and 0.18% and 0.13% for the high-sensitivity assay (n = 3878). There was no significant reduction in outlier rate between the two assays (χ(2) = 0.034, P = 0.854). Fifty-six percent of outliers occurred in samples where cTn was an 'add-on' test (and was stored and refrigerated prior to assay). Despite recent improvements in cTn methods, outliers (including critical outliers) still occur at a low rate in both a contemporary and high-sensitivity cTnI assay. Laboratory and clinical staff should be aware of this potential analytical error, particularly in samples with suboptimal sample handling such as add-on tests. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Guidelines and Data to Support Plans for Reallocating Food during Crisis Relocation. Regional Appendix. FEMA Region VI,

DTIC Science & Technology

1982-09-01

POPLAR BLUFF MO 2 1 0 0 0 2,2-50 JONESBORO GROCER CO JOIES3ORO AR 1 I 0 0 0 0 7-0 MALONE AND HIDE INC MEMPHIS TN 4 2 I I 0 0 6,000 WETTERAU FOODS INC SCOTT...five states in FEMA Region VI ( Louisiana, Oklahoma and Texas) are largely P self-sufficient, warehousing between 75% to 97% of their food supplies...within their own borders. New Mexico and Arkansas are heavily dependent on out-of-state warehouses for food shipments, with less than one-third of their

77 FR 50921 - Safety Zone: Bay Bridge Load Transfer Safety Zone, San Francisco Bay, San Francisco, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-23

...-AA00 Safety Zone: Bay Bridge Load Transfer Safety Zone, San Francisco Bay, San Francisco, CA AGENCY... the Bay Bridge Load Transfer Safety Zone from August 1, 2012 through October 31, 2012. This safety... Bay Bridge from the temporary suspension arrangement to the permanent suspension arrangement, the...

Grímsvötn Volcano Injects Ash into the Stratosphere

NASA Image and Video Library

2011-05-24

NASA Terra spacecraft captured this image of Grímsvötn, the most active of Iceland volcanoes, which began erupting around 5:30 p.m. local time 1730 UTC on Saturday, May 21, 2011, east of the capital city of Reykjavik.

[Nutrient Characteristics and Nitrogen Forms of Rhizosphere Soils Under Four Typical Plants in the Littoral Zone of TGR].

PubMed

Wang, Xiao-feng; Yuan, Xing-zhong; Liu, Hong; Zhang, Lei; Yu, Jian-jun; Yue, Jun-sheng

2015-10-01

The Three Gorges Reservoir (TGR), which is the largest water conservancy project ever built in tne world, produced a drawdown area of about 348.93 km2 because of water level control. The biological geochemical cycle of the soil in the drawdown zone has been changed as the result of long-term winter flooding and summer drought and vegetation covering. The loss of soil nitrogen in the drawdown zone poses a threat to the water environmental in TGR. Pengxi river, is an important anabranch, which has the largest drawdown area has been selected in the present study. The four typical vegetation, contained Cynodon dactylon, Cyperus rotundus, Anthium sibiricum and Zea mays L. as the control, were studied to measure nutrient characteristics and nitrogen forms of rhizosphere and non-rhizosphere soils in three distribution areas with different soil types (paddy soil, purple soil and fluvo-aquic soils). The variables measured included organic matter (OM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), hydrolysis N, available P and available K, pH, ion-exchangeable N (IEE-N), weak acid extractable N (CF-N) , iron-manganese oxides N (IMOF-N), organic matter sulfide N (OSF-N), added up four N forms for total transferable N (TF-N) and TN minus TF-N for non-transferable N (NTF-N). The results showed: (1) pH of rhizosphere soil was generally lower than that of non-rhizosphere soil under different vegetation in different type soils because the possible organic acid and H+ released form plant roots and cation absorption differences, and the OM, TP, TN and hydrolysis N of rhizosphere soil were generally higher than those of non-rhizosphere soil, and that the enrichment ratio (ER) of all the four nutrient indicators showed Cyperus rotundus > Cynodon dactylon > Zea mays L. > Anthium sibiricum. Available P showed enrichment in the rhizosphere of three natural vegetations but lose under corn, and available K, TK showed different ER in different conditions. (2) IEF-N CF

Hydrogeology, water quality, and potential for transport of organochlorine pesticides in ground water at the North Hollywood Dump, Memphis, Tennessee

USGS Publications Warehouse

Broshears, R.E.; Bradley, M.W.

1992-01-01

Geologic, hydrologic, and water-quality data indicate that ground-water contamination is confined to shallow horizons within the unconfined aquifer underlying the North Hollywood Dump in Memphis, Tennessee. The dump is a closed municipal-industrial landfill that has been ranked as Tennessee's potentially most dangerous hazardous-waste site. Toxic constituents of concern at the dump include residues from the manufacture of organochlorine pesticides. The dump overlies an unconfined aquifer of unconsolidated sands, silts, and clays. During average hydrologic conditions, ground waterflows beneath the dump at a mean velocity of approximately 3 feet per day and discharges to the Wolf River. Leachate from the dump mixes with underlying ground water, resulting in increased concentrations of dissolved solids and organic carbon downgradient from the dump. The mobility of chlordane, a representative organochlorine pesticide, is limited by its low solubility and its strong affinity for sand, silt, and clays of the aquifer. Degradation of chlordane may occur slowly, if at all, in the aquifer. Based on estimates of mean ground-water velocity and retardation of the pesticide due to sorption, mean travel times for chlordane migrating from the dump to the ground-water discharge zone are of the order of 50 to 500 years. Simulations of chlordane concentration resulting from the discharge of contaminated ground water and complete mixing in the Wolf River are sensitive to assumptions about chlordane persistence in the unconfined aquifer. If the half life of chlordane in the aquifer is assumed to be 30 years or less, the simulated concentration of chlordane in the Wolf River under average flow conditions is less than the most stringent water-quality criterion.

Comparison of Friction Characteristics on TN and VA Mode Alignment Films with Friction Force Microscopy

NASA Astrophysics Data System (ADS)

Kwak, Musun; Chung, Hanrok; Kwon, Hyukmin; Kim, Jehyun; Han, Daekyung; Yi, Yoonseon; Lee, Sangmun; Lee, Chulgu; Cha, Sooyoul

Using frictional force microscopy (FFM), the friction surface characteristics were compared between twisted nematic (TN) mode and vertical alignment (VA) mode alignment films (AFs). The friction asymmetry was detected depending on temperature conditions on TN mode AF, but not on VA mode AF. The difference between two modes was explained by leaning intermolecular repulsion caused by the pre-tilt angle uniformity and the density of side chain. No level difference according to temperature conditions appeared when the pre-tilt angle were measured after liquid crystal (LC) injection.

47 CFR 22.503 - Paging geographic area authorizations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... (Birmingham) 36, 74, 78-79. 25 (Nashville) 71. 26 (Memphis-Jackson) 73, 75-77. 27 (New Orleans-Baton Rouge) 80...: MEAs EAs 1 (Boston) 1-3. 2 (New York City) 4-7, 10. 3 (Buffalo) 8. 4 (Philadelphia) 11-12. 5...

Dynamic Equilibrium of Cardiac Troponin C's Hydrophobic Cleft and Its Modulation by Ca2+ Sensitizers and a Ca2+ Sensitivity Blunting Phosphomimic, cTnT(T204E).

PubMed

Schlecht, William; Dong, Wen-Ji

2017-10-18

Several studies have suggested that conformational dynamics are important in the regulation of thin filament activation in cardiac troponin C (cTnC); however, little direct evidence has been offered to support these claims. In this study, a dye homodimerization approach is developed and implemented that allows the determination of the dynamic equilibrium between open and closed conformations in cTnC's hydrophobic cleft. Modulation of this equilibrium by Ca 2+ , cardiac troponin I (cTnI), cardiac troponin T (cTnT), Ca 2+ -sensitizers, and a Ca 2+ -desensitizing phosphomimic of cTnT (cTnT(T204E) is characterized. Isolated cTnC contained a small open conformation population in the absence of Ca 2+ that increased significantly upon the addition of saturating levels of Ca 2+ . This suggests that the Ca 2+ -induced activation of thin filament arises from an increase in the probability of hydrophobic cleft opening. The inclusion of cTnI increased the population of open cTnC, and the inclusion of cTnT had the opposite effect. Samples containing Ca 2+ -desensitizing cTnT(T204E) showed a slight but insignificant decrease in open conformation probability compared to samples with cardiac troponin T, wild type [cTnT(wt)], while Ca 2+ sensitizer treated samples generally increased open conformation probability. These findings show that an equilibrium between the open and closed conformations of cTnC's hydrophobic cleft play a significant role in tuning the Ca 2+ sensitivity of the heart.

Correlating Calcium Binding, Förster Resonance Energy Transfer, and Conformational Change in the Biosensor TN-XXL

PubMed Central

Geiger, Anselm; Russo, Luigi; Gensch, Thomas; Thestrup, Thomas; Becker, Stefan; Hopfner, Karl-Peter; Griesinger, Christian; Witte, Gregor; Griesbeck, Oliver

2012-01-01

Genetically encoded calcium indicators have become instrumental in imaging signaling in complex tissues and neuronal circuits in vivo. Despite their importance, structure-function relationships of these sensors often remain largely uncharacterized due to their artificial and multimodular composition. Here, we describe a combination of protein engineering and kinetic, spectroscopic, and biophysical analysis of the Förster resonance energy transfer (FRET)-based calcium biosensor TN-XXL. Using fluorescence spectroscopy of engineered tyrosines, we show that two of the four calcium binding EF-hands dominate the FRET output of TN-XXL and that local conformational changes of these hands match the kinetics of FRET change. Using small-angle x-ray scattering and NMR spectroscopy, we show that TN-XXL changes from a flexible elongated to a rigid globular shape upon binding calcium, thus resulting in FRET signal output. Furthermore, we compare calcium titrations using fluorescence lifetime spectroscopy with the ratiometric approach and investigate potential non-FRET effects that may affect the fluorophores. Thus, our data characterize the biophysics of TN-XXL in detail and may form a basis for further rational engineering of FRET-based biosensors. PMID:22677394

78 FR 48078 - Proposed Amendment of Class E Airspace; Tazewell, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-07

...: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: This action proposes to amend Class E Airspace at Tazewell, TN, as new Standard Instrument Approach Procedures have been developed at New Tazewell Municipal Airport. This action would enhance the safety and...

78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-23

...-Cities Regional Airport, Tri-Cities, TN. Exclusionary language was omitted in the final rule published in... herein to include the corrective language. Since the regulatory text, as currently described, penetrates... FAA has determined that this regulation only involves an established body of technical regulations for...

Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi

2010-06-20

Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZ{alpha}-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cellsmore » as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.« less

Carcinoma autoantigens T and Tn and their cleavage products interact with Gal/GalNAc-specific receptors on rat Kupffer cells and hepatocytes.

PubMed

Schlepper-Schäfer, J; Springer, G F

1989-10-09

We studied interactions of isolated Thomsen-Friedenreich (T)- and Tn-specific glycoproteins with the Gal/GalNAc-specific receptors on rat Kupffer cells and compared them to those with rat hepatocytes. Immunoreactive T and Tn are specific pancarcinoma epitopes. Electron microscopy of gold-labelled T and Tn antigens revealed their specific binding to Kupffer cells, followed by their uptake via the coated pit/vesicle pathway of receptor-mediated endocytosis. Preincubation of Kupffer cells with GalNAc and GalNAc-BSA, but not GlcNAc or GlcNAc-BSA specifically inhibited binding of the T and Tn glycoproteins. Desialylated, isologous erythrocytes (T RBC) are known to bind to the Gal/GalNAc receptors of rat Kupffer cells and hepatocytes. This attachment was specifically inhibited by T and Tn in a concentration-dependent manner: 50% T RBC-Kupffer cell contacts were inhibited at 8.5.10(-6) mM T and 8.5.10(-5) mM Tn antigen concentrations, respectively. The corresponding figures for hepatocytes were 6.10(-6) mM T and 1.2.10(-6) mM Tn antigen. Amino-terminal cleavage products of the T glycoprotein, possessing clusters terminating in non-reducing Gal/GalNAc, inhibited T RBC binding to Kupffer cells and hepatocytes usually at 10(-2) to 10(-5) mM concentrations, whereas GalNAc, galactose and galactose glycosides inhibited at millimolar concentrations. Galactose-unrelated carbohydrates were inactive at concentrations greater than or equal to 50 mM.

77 FR 23395 - Safety Zones; Recurring Events in Captain of the Port New York Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-19

... Safety Zone....... position 40[deg]46'11.8'' N, 33 CFR 165.160(5.4) 074[deg]00'14.8'' W (NAD 1983...'' W (NAD 1983), approximately 380 yards west of Pier 54, Manhattan, NY. Date: June 24, 2012. Time: 10... Zone located in approximate 33 CFR 165.160(2.5) position 40[deg]30'04'' N 074[deg]15'35'' W (NAD 1983...

77 FR 70964 - Security Zone, Potomac and Anacostia Rivers; Washington, DC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-28

... 1625-AA87 Security Zone, Potomac and Anacostia Rivers; Washington, DC AGENCY: Coast Guard, DHS. ACTION... to establish a security zone during activities associated with the Presidential Inauguration in... extends the southern boundary of the proposed security zone. This rule prohibits vessels and people from...

75 FR 65584 - Proposed Amendment of Class E Airspace; Savannah, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-26

...-1047; Airspace Docket No. 10-ASO-37] Proposed Amendment of Class E Airspace; Savannah, TN AGENCY...; Telephone: 1- 800-647-5527; Fax: 202-493-2251. You must identify the Docket Number FAA-2010-1047; Airspace... the proposal. Communications should identify both docket numbers (FAA Docket No. FAA-2010-1047...

IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration.

PubMed

Stewart, Barry J; Wardle, Simon J; Haniford, David B

2002-08-15

The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This strand transfer transpososome (STT) does appear to support an intermolecular disintegration reaction, but only at a very low level. Strikingly, assembly of the STT is not dependent on IHF, a host protein that is required for de novo assembly of all previously characterized Tn10 transpososomes. We suggest that disintegration substrates are able to form both transposon end and target type contacts with transposase because of their enhanced conformational flexibility. This probably allows the conformation of DNA within the complex that prevents the destructive disintegration reaction, and is responsible for relaxing the DNA sequence requirements for STT formation relative to other Tn10 transpososomes.

IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration

PubMed Central

Stewart, Barry J.; Wardle, Simon J.; Haniford, David B.

2002-01-01

The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This strand transfer transpososome (STT) does appear to support an intermolecular disintegration reaction, but only at a very low level. Strikingly, assembly of the STT is not dependent on IHF, a host protein that is required for de novo assembly of all previously characterized Tn10 transpososomes. We suggest that disintegration substrates are able to form both transposon end and target type contacts with transposase because of their enhanced conformational flexibility. This probably allows the conformation of DNA within the complex that prevents the destructive disintegration reaction, and is responsible for relaxing the DNA sequence requirements for STT formation relative to other Tn10 transpososomes. PMID:12169640

77 FR 41688 - Security Zones; 2012 Republican National Convention, Captain of the Port St. Petersburg Zone...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-16

...-AA87 Security Zones; 2012 Republican National Convention, Captain of the Port St. Petersburg Zone... prohibited unless authorized by the Captain of the Port St. Petersburg or a designated representative. DATES... temporary final rule, call or email Marine Science Technician First Class Nolan L. Ammons, Sector St...

77 FR 19095 - Security Zone; USCGC STRATTON Commissioning Ceremony, Alameda, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-30

...-AA87 Security Zone; USCGC STRATTON Commissioning Ceremony, Alameda, CA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary security zone in the navigable waters of the San Francisco Bay, Alameda, CA within the San Francisco Captain of the Port (COTP) Zone...

77 FR 45333 - Reorganization of Foreign-Trade Zone 148 Under Alternative Site Framework; Knoxville, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-31

... Sites 3, 4 and 5 would be categorized as magnet sites, existing Sites 2, 6 and 7 would be categorized as... sunset provision for magnet sites that would terminate authority for Sites 4 and 5 if not activated by...

Concentrations of selected trace inorganic constituents and synthetic organic compounds in the water-table aquifers in the Memphis area, Tennessee

USGS Publications Warehouse

McMaster, B.W.; Parks, William Scott

1988-01-01

Water quality samples for analysis of selected trace inorganic constituents and synthetic organic compounds were collected from 29 private or observation wells in alluvium and fluvial deposits of Quaternary and Tertiary Age. The alluvium and fluvial deposits are the water table aquifers in the Memphis area. In addition, nine wells were installed in Memphis Light, Gas and Water Division well fields so that samples could be collected and analyzed to characterize the quality of water in the fluvial deposits at these well fields. Samples from seven of these wells (two were dry) were analyzed for major constituents and properties of water as well as for selected trace inorganic constituents and synthetic organic compounds. Analyses of the water from most of the 36 wells sampled indicated ranges in concentration values for the trace inorganic constituents that agreed with those previously known, although some new maximum values were established. The analysis of water from four wells indicated that the water is or may be contaminated. Concentrations of barium (1,400 micrograms/L -- ug/L), strontium (1,100 ug/L), and arsenic (15 ug/L), along with specific conductance (1,420 microsiemens/centimeter--us/cm) were in water from one well in the alluvium. Low concentrations (0.02 to 0.04 ug/L) of the pesticides aldrin, DDT, endosulfan, and perthane were present in water from two wells in the fluvial deposits. Water from one of these wells also contained 1,1,1 trichloroethane (4.4 ug/L). Analysis of water from another well in the fluvial deposits indicated values for specific conductance (1,100 uS/cm), alkalinity (508 milligrams per liter -- mg/L -- as CaCO3), hardness (550 mg/L as CaCO3), chloride (65 mg/L), and barium (240 ug/L) that are high for water from the fluvial deposits. (USGS)

Incised valley fill interpretation for Mississippian Black Hand Sandstone, Appalachian Basin, USA: Implications for glacial eustasy at Kinderhookian-Osagean (Tn2-Tn3) boundary

USGS Publications Warehouse

Matchen, D.L.; Kammer, T.W.

2006-01-01

Lower Mississippian strata of east-central Ohio are predominantly fine-grained marine deposits of the Cuyahoga and Logan formations. Within these sediments is the Black Hand Sandstone of the Cuyahoga Formation. The Black Hand Sandstone is a multistory, crossbedded, coarse-grained conglomeratic sandstone. The contact between the Black Hand Sandstone and the subjacent Cuyahoga Formation is sharp and scoured, with intraclasts of the Cuyahoga Formation incorporated into the basal Black Hand Sandstone. The Black Hand Sandstone was previously thought to represent a distributary channel deposit; however, the combination of lithofacies and architectural elements indicates deposition in a braided stream setting. The Cuyahoga Formation was deposited in a shallow marine setting. The erosional basal contact of the Black Hand Sandstone and the juxtaposition of fluvial and marine sediments suggests a sequence boundary. The geographic distribution of the Black Hand Sandstone combined with the evidence for a sequence boundary suggests deposition in an incised valley. The age of the Black Hand Sandstone is key to inferring the causes of valley incision. The Black Hand Sandstone is nearly devoid of body fossils, necessitating a biostratigraphic analysis of the surrounding Cuyahoga and Logan formations. Analysis indicates the Logan Formation is early Osagean age. Data from the Cuyahoga Formation suggest a Kinderhookian age with a possible transition to the Osagean in the uppermost Cuyahoga Formation. This constrains the age of the Black Hand Sandstone to the transition at the Kinderhookian-Osagean boundary. Recent reports indicate late Kinderhookian (Tournaisian, Tn2) Gondwanan glaciation based upon tillites and sharp excursions in stable-isotope curves. A glacio-eustatic fall in sea level is inferred to have caused incision of the Cuyahoga Formation, followed by deposition of the Black Hand Sandstone and Logan Formation during the subsequent sea level rise. The associated

KPC-4 Is Encoded within a Truncated Tn4401 in an IncL/M Plasmid, pNE1280, Isolated from Enterobacter cloacae and Serratia marcescens

PubMed Central

Bryant, Kendall A.; Van Schooneveld, Trevor C.; Thapa, Ishwor; Bastola, Dhundy; Williams, Laurina O.; Safranek, Thomas J.; Hinrichs, Steven H.; Rupp, Mark E.

2013-01-01

We describe the transfer of blaKPC-4 from Enterobacter cloacae to Serratia marcescens in a single patient. DNA sequencing revealed that KPC-4 was encoded on an IncL/M plasmid, pNE1280, closely related to pCTX-M360. Further analysis found that KPC-4 was encoded within a novel Tn4401 element (Tn4401f) containing a truncated tnpA and lacking tnpR, ISKpn7 left, and Tn4401 IRL-1, which are conserved in other Tn4401 transposons. This study highlights the continued evolution of Tn4401 transposons and movement to multiple plasmid backbones that results in acquisition by multiple species of Gram-negative bacilli. PMID:23070154

Comparison of the new high sensitive cardiac troponin T with myoglobin, h-FABP and cTnT for early identification of myocardial necrosis in the acute coronary syndrome.

PubMed

Kurz, Kerstin; Giannitsis, Evangelos; Becker, Maike; Hess, Georg; Zdunek, Dietmar; Katus, Hugo A

2011-03-01

We sought to determine the performance of the new high sensitivity cardiac troponin T assay (TnThs) for early diagnosis of myocardial infarction in patients with suspected acute coronary syndrome (ACS) and compare it with the fourth generation cTnT assay, myoglobin and heart-type fatty acid binding protein (h-FABP). Ninety-four patients with diagnosis of suspected ACS without ST-segment elevation admitted to our chest pain unit were included. Patients were divided according to time from onset of symptoms to presentation into an early presenter group (<4 h) and a late presenter group (≥4 h). A median of six samples (range 2-8) were available per patient. The diagnostic performance of TnThs was assessed using ROC analysis. Areas under the curve (AUC) of baseline and follow-up results of TnThs, cTnT, myoglobin, and h-FABP were compared using c statistics. The TnThs assay allows an excellent prediction of non-ST-segment elevation myocardial infarction (non-STEMI) at presentation, particularly among late presenters. A follow-up sample improves diagnostic performance in a time-dependent manner. The AUC of TnThs was superior to cTnT at all time points. The performance of TnThs was at least as good as myoglobin and h-FABP at presentation and during follow-up. A baseline sample of TnThs allows an earlier prediction of non-STEMI than the less sensitive and precise fourth generation cTnT assay. Probably, this excellent performance of TnThs at baseline and follow-up could obviate the need for other early markers of necrosis in future.

77 FR 35852 - Safety Zones; Multiple Firework Displays in Captain of the Port, Puget Sound Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... 13045, Protection of Children from Environmental Health Risks and Safety Risks. This rule is not an economically significant rule and does not create an environmental risk to health or risk to safety that may... 1625-AA00 Safety Zones; Multiple Firework Displays in Captain of the Port, Puget Sound Zone AGENCY...

Radioprotection of the Brain White Matter by Mn(III) N-Butoxyethylpyridylporphyrin-Based Superoxide Dismutase Mimic MnTnBuOE-2-PyP5+

PubMed Central

Weitzel, Douglas H.; Tovmasyan, Artak; Ashcraft, Kathleen A.; Rajic, Zrinka; Weitner, Tin; Liu, Chunlei; Li, Wei; Buckley, Anne F.; Prasad, Mark R.; Young, Kenneth H.; Rodriguiz, Ramona M.; Wetsel, William C.; Peters, Katherine B.; Spasojevic, Ivan; Herndon, James E.; Batinic-Haberle, Ines; Dewhirst, Mark W.

2015-01-01

Cranial irradiation is a standard therapy for primary and metastatic brain tumors. A major drawback of radiotherapy (RT), however, is long-term cognitive loss that affects quality of life. Radiation-induced oxidative stress in normal brain tissue is thought to contribute to cognitive decline. We evaluated the effectiveness of a novel mimic of superoxide dismutase enzyme (SOD), MnTnBuOE-2-PyP5+ (Mn(III) meso-tetrakis(N-n-butoxyethylpyridinium-2-yl)porphyrin), to provide long-term neuroprotection following 8 Gy of whole brain irradiation. Long-term RT damage can only be assessed by brain imaging and neurocognitive studies. C57BL/6J mice were treated with MnTnBuOE-2-PyP5+ before and after RT and evaluated three months later. At this time point, drug concentration in the brain was 25 nmol/L. Mice treated with MnTnBuOE-2-PyP5+/RT exhibited MRI evidence for myelin preservation in the corpus callosum compared with saline/RT treatment. Corpus callosum histology demonstrated a significant loss of axons in the saline/RT group that was rescued in the MnTnBuOE-2-PyP5+/RT group. In addition, the saline/RT groups exhibited deficits in motor proficiency as assessed by the rotorod test and running wheel tests. These deficits were ameliorated in groups treated with MnTnBuOE-2-PyP5+/RT. Our data demonstrate that MnTnBuOE-2-PyP5+ is neuroprotective for oxidative stress damage caused by radiation exposure. In addition, glioblastoma cells were not protected by MnTnBuOE-2-PyP5+ combination with radiation in vitro. Likewise, the combination of MnTnBuOE-2-PyP5+ with radiation inhibited tumor growth more than RT alone in flank tumors. In summary, MnTnBuOE-2-PyP5+ has dual activity as a neuroprotector and a tumor radiosensitizer. Thus, it is an attractive candidate for adjuvant therapy with RT in future studies with patients with brain cancer. PMID:25319393

Transportations Systems Modeling and Applications in Earthquake Engineering

DTIC Science & Technology

2010-07-01

49 Figure 6 PGA map of a M7.7 earthquake on all three New Madrid fault segments (g)............... 50...Memphis, Tennessee. The NMSZ was responsible for the devastating 1811-1812 New Madrid earthquakes , the largest earthquakes ever recorded in the...Figure 6 PGA map of a M7.7 earthquake on all three New Madrid fault segments (g) Table 1 Fragility parameters for MSC steel bridge (Padgett 2007

Physicochemical and toxicological profiling of ash from the 2010 and 2011 eruptions of Eyjafjallajökull and Grímsvötn volcanoes, Iceland using a rapid respiratory hazard assessment protocol.

PubMed

Horwell, C J; Baxter, P J; Hillman, S E; Calkins, J A; Damby, D E; Delmelle, P; Donaldson, K; Dunster, C; Fubini, B; Kelly, F J; Le Blond, J S; Livi, K J T; Murphy, F; Nattrass, C; Sweeney, S; Tetley, T D; Thordarson, T; Tomatis, M

2013-11-01

The six week eruption of Eyjafjallajökull volcano in 2010 produced heavy ash fall in a sparsely populated area of southern and south eastern Iceland and disrupted European commercial flights for at least 6 days. We adopted a protocol for the rapid analysis of volcanic ash particles, for the purpose of informing respiratory health risk assessments. Ash collected from deposits underwent a multi-laboratory physicochemical and toxicological investigation of their mineralogical parameters associated with bio-reactivity, and selected in vitro toxicology assays related to pulmonary inflammatory responses. Ash from the eruption of Grímsvötn, Iceland, in 2011 was also studied. The results were benchmarked against ash from Soufrière Hills volcano, Montserrat, which has been extensively studied since the onset of eruptive activity in 1995. For Eyjafjallajökull, the grain size distributions were variable: 2-13 vol% of the bulk samples were <4 µm, with the most explosive phases of the eruption generating abundant respirable particulate matter. In contrast, the Grímsvötn ash was almost uniformly coarse (<3.5 vol%<4 µm material). Surface area ranged from 0.3 to 7.7 m2 g(-1) for Eyjafjallajökull but was very low for Grímsvötn (<0.6 m2 g(-1)). There were few fibre-like particles (which were unrelated to asbestos) and the crystalline silica content was negligible in both eruptions, whereas Soufrière Hills ash was cristobalite-rich with a known potential to cause silicosis. All samples displayed a low ability to deplete lung antioxidant defences, showed little haemolysis and low acute cytotoxicity in human alveolar type-1 like epithelial cells (TT1). However, cell-free tests showed substantial hydroxyl radical generation in the presence of hydrogen peroxide for Grímsvötn samples, as expected for basaltic, Fe-rich ash. Cellular mediators MCP-1, IL-6, and IL-8 showed chronic pro-inflammatory responses in Eyjafjallajökull, Grímsvötn and Soufrière Hills samples

The relationship between the expression of TN and the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis

PubMed Central

Jiang, Guohua; Zhang, Yinshun; Sun, Xianjie

2018-01-01

Abstract Background: This study was conducted with the aim to investigate the relationship between Tetranectin (TN) and efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis. Methods: Ninety-two patients with traumatic kyphosis admitted in our hospital from February 2014 to June 2016 were included whose serum TN levels were examined by ELISA. Using the mean level of TN as cut-off value, patients were classified into TN high level group (group I) and TN low level group (group II). The observation indexes, including operation time, intra-operational loss of blood, Cobb angle, postoperative complications and recurrence rate of kyphosis within post-operational 6 months were recorded for comparison. Results: TN level was significantly higher in group I [(6.19 ± 0.33) μmol/L] than that in group II [(5.29 ± 0.34) μmol/L] (P < .05). There was no significant difference in average age, sex, lesion site and average time from injury to operation between the two groups (all P > 0.05). Compared to group II, operation time in group I was significantly shortened (5.02 ± 1.15 VS 4.58 ± 0.53, P = .023), the intra-operational loss of blood decreased (2418.56 ± 362.06 VS 2235.84 ± 325.63, P = .013), post-operational Cobb angle decreased (11.10 ± 1.31 VS 6.93 ± 1.04, P = .000), and the incidence of postoperative complications (nail-breaking, rod-breaking and looseness) and recurrence rate decreased (18.8% VS 4.5%, P = .036; 10.4% VS 0.0%, P = .028). Conclusion: Serum TN level is proved to be related to the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis, and may serve as a possible indicator for clinical treatment. PMID:29384840

The relationship between the expression of TN and the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis.

PubMed

Jiang, Guohua; Zhang, Yinshun; Sun, Xianjie

2018-02-01

This study was conducted with the aim to investigate the relationship between Tetranectin (TN) and efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis. Ninety-two patients with traumatic kyphosis admitted in our hospital from February 2014 to June 2016 were included whose serum TN levels were examined by ELISA. Using the mean level of TN as cut-off value, patients were classified into TN high level group (group I) and TN low level group (group II). The observation indexes, including operation time, intra-operational loss of blood, Cobb angle, postoperative complications and recurrence rate of kyphosis within post-operational 6 months were recorded for comparison. TN level was significantly higher in group I [(6.19 ± 0.33) μmol/L] than that in group II [(5.29 ± 0.34) μmol/L] (P < .05). There was no significant difference in average age, sex, lesion site and average time from injury to operation between the two groups (all P > 0.05). Compared to group II, operation time in group I was significantly shortened (5.02 ± 1.15 VS 4.58 ± 0.53, P = .023), the intra-operational loss of blood decreased (2418.56 ± 362.06 VS 2235.84 ± 325.63, P = .013), post-operational Cobb angle decreased (11.10 ± 1.31 VS 6.93 ± 1.04, P = .000), and the incidence of postoperative complications (nail-breaking, rod-breaking and looseness) and recurrence rate decreased (18.8% VS 4.5%, P = .036; 10.4% VS 0.0%, P = .028). Serum TN level is proved to be related to the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis, and may serve as a possible indicator for clinical treatment.

77 FR 51471 - Safety Zone; Swim Around Charleston, Charleston, SC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-24

...-AA00 Safety Zone; Swim Around Charleston, Charleston, SC AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary moving safety zone during the Swim Around Charleston, a swimming race occurring on waters of the Wando River, the Cooper River, Charleston...

77 FR 22495 - Safety Zone; Lake Pontchartrain, New Orleans, LA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-16

...-AA00 Safety Zone; Lake Pontchartrain, New Orleans, LA AGENCY: Coast Guard, DHS. ACTION: Temporary final... from the South shores of Lake Pontchartrain adjacent to the East bank of the Lakefront Airport runways... proposed rulemaking (NPRM) entitled Safety Zone, Lake Pontchartrain, New Orleans, LA in the Federal...

Tn (N-acetyl-d-galactosamine-O-serine/threonine) immunization protects against hyperoxia-induced lung injury in adult mice through inhibition of the nuclear factor kappa B activity.

PubMed

Chen, Chung-Ming; Hwang, Jaulang; Chou, Hsiu-Chu; Shiah, Her-Shyong

2018-06-01

Prolonged hyperoxia exposure leads to inflammation and acute lung injury. Since hyperoxia activates nuclear factor kappa B (NF-κB) and proinflammatory mediators in lung fibroblasts and murine lungs, and proinflammatory cytokines upregulate Tn (N-acetyl-d-galactosamine-O-serine/threonine) expression in human gingival fibroblasts. We hypothesized connections exist between Tn expression and inflammation regulation. Thus, we immunized adult mice with Tn antigen to examine whether Tn vaccine can protect against hyperoxia-induced lung injury by inhibiting NF-κB activity and cytokine expression through the action of anti-Tn antibodies. Five-week-old female C57BL/6NCrlBltw mice were subcutaneously immunized with Tn antigen four times at biweekly intervals, and one additional immunization was performed at 1 week after the fourth immunization. Four days after the last immunization, mice were exposed to room air (RA) or hyperoxia (100% O 2 ) for up to 96 h. Four study groups were examined: carrier protein + RA (n = 6), Tn vaccine + RA (n = 6), carrier protein + O 2 (n = 6), and Tn vaccine + O 2 (n = 5). We observed that hyperoxia exposure reduced body weight, increased alveolar protein and cytokine (interleukin-6 and tumor necrosis factor-α) levels, increased mean linear intercept (MLI) values and lung injury scores, and increased lung NF-κB activity. By contrast, Tn immunization increased serum anti-Tn antibody titers and reduced the cytokine levels, MLI values, and lung injury scores. Furthermore, the alleviation of lung injury was accompanied by a reduction in NF-κB activity. Therefore, we proposed that Tn immunization attenuates hyperoxia-induced lung injury in adult mice by inhibiting the NF-κB activity. Copyright © 2018 Elsevier B.V. All rights reserved.

Sediment record of environmental change at Lake Lop Nur (Xinjiang, NW China) from 13.0 to 5.6 cal ka BP

NASA Astrophysics Data System (ADS)

Wang, Jingzhong; Jia, Hongjuan

2017-09-01

Lake Lop Nur is located in the eastern part of the Tarim Basin in Xinjiang, northwestern China. A 220-cm-long sediment core was collected from the center of the ear-shaped depression forming the basin and dated with AMS14C. Grain size, total organic matter (TOC), total nitrogen (TN), and TOC/TN (C/N) analyses were used to reconstruct climatic conditions from 13.0 to 5.6 cal ka BP. The results showed five main climatic stages. Zone I (13.0-11.3 cal ka BP) was a wet-dry environment, whereas Zone II (11.3-8.9 cal ka BP) consisted of a primarily wet environment. Zone III (8.9-7.7 cal ka BP) was subdivided into Zone IIIa (8.9-8.2 cal ka BP) that indicated lake constriction and dry climate, and Zone IIIb (8.2-7.7 cal ka BP) in which the proxies indicated wet conditions. In Zone IV (7.7-6.6 cal ka BP), the climate presented a bit wet conditions. In Zone V (6.6-5.6 cal ka BP), abundant glauberite is present in the sediment and silt dominates the lithology; these results indicate the lake shrank and the overall climate was dry. Abrupt environmental events were also identified, including six dry events at 11.0, 10.5, 9.3, 8.6, 8.2, and 7.6 cal ka BP and one flood event from 7.8 to 7.7 cal ka BP in the Early-Middle Holocene.

Soil organic carbon and total nitrogen pools in permafrost zones of the Qinghai-Tibetan Plateau.

PubMed

Zhao, Lin; Wu, Xiaodong; Wang, Zhiwei; Sheng, Yu; Fang, Hongbing; Zhao, Yonghua; Hu, Guojie; Li, Wangping; Pang, Qiangqiang; Shi, Jianzong; Mo, Bentian; Wang, Qian; Ruan, Xirui; Li, Xiaodong; Ding, Yongjian

2018-02-26

There are several publications related to the soil organic carbon (SOC) on the Qinghai-Tibetan Plateau (QTP). However, most of these reports were from different parts of the plateau with various sampling depth. Here, we present the results from a systematic sampling and analysis of 200 soil pits. Most of the pits were deeper than 2 m from an east-west transect across the plateau. The SOC and total nitrogen (TN) pools of the 148 × 10 4  km 2 , the area of the permafrost zone, for the upper 2 m soils calculated from the vegetation map were estimated to be 17.07 Pg (interquartile range: 11.34-25.33 Pg) and 1.72 Pg (interquartile range: 1.08-2.06 Pg), respectively. We also predicted the distribution of land cover types in 2050 and 2070 using decision tree rules and climate scenarios, and then predicted SOC and TN pools of this region. The results suggested that the SOC and TN pools will decrease in the future. The results not only contribute to the carbon and nitrogen storage and stocks in the permafrost regions as a whole but most importantly, to our knowledge of the possible changes of C and N storage on the QTP in the future.

Today's CIO: catalyst for managed care change.

PubMed

Sanchez, P

1997-05-01

As the impact of managed care increases and capitation becomes all pervasive, healthcare providers' attention to cost control will intensify. For integrated delivery networks (IDNs) to be competitive, today's CIO must leverage managed care as a catalyst for change, and use a sophisticated information system toolset as the means to an integrated end. An area many CIOs target for fast results and maximum cost savings in resource management. This article reviews how Dick Escue, chief information officer at Baptist Memorial Health Care Corporation (Memphis, TN), uses electronic information management systems to integrate and conserve the resources of Baptist's widespread healthcare organization.

In-house preparation of lectin panel and detection of Tn polyagglutination.

PubMed

Das, Sudipta Sekhar

2015-01-01

Polyagglutination is a condition in which red cells are agglutinated by ABO-compatible adult human sera, but not by cord blood sera and may be acquired or inherited. Lectins are invaluable reagents in the investigation of red cells polyagglutination. We prepared in-house lectin panel and confirmed Tn polyagglutination in a pregnant lady. The lady was anemic and refused blood transfusion elsewhere due to serological discrepancy. We found ABO discrepancy and an incompatible minor cross-match in the initial investigation and suspected polyagglutination. Confirmation of polyagglutination was done using adult and cord sera. We then used the in-house lectin panels to detect the type of polyagglutination. The agglutination pattern with the various lectins was suggestive of Tn polyagglutination, which was further supported by the enzyme study. Most blood banks in India lack commercial lectin panels because of cost and procurement difficulty. Lectins play an important role in the diagnosis and differentiation of polyagglutination and immunohematological management of patient. The important and basic lectins can be prepared in-house using specific raw seeds following standardized protocol.

77 FR 11423 - Safety Zone; Magothy River, Sillery Bay, MD

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-27

...-AA00 Safety Zone; Magothy River, Sillery Bay, MD AGENCY: Coast Guard, DHS. ACTION: Notice of proposed rulemaking. SUMMARY: The Coast Guard proposes to establish a safety zone in certain waters of the Magothy... in the Magothy River, in Sillery Bay, Maryland. The proposed rule is needed to control movement...

Novel Structure of Enterococcus faecium-Originated ermB-Positive Tn1546-Like Element in Staphylococcus aureus.

PubMed

Wan, Tsai-Wen; Hung, Wei-Chun; Tsai, Jui-Chang; Lin, Yu-Tzu; Lee, Hao; Hsueh, Po-Ren; Lee, Tai-Fen; Teng, Lee-Jene

2016-10-01

We determined the resistance determinants in 274 erythromycin-resistant methicillin-susceptible Staphylococcus aureus (MSSA) isolates during a 13-year period, 2000 to 2012. The resistance phenotypes, inducible macrolide-lincosamide-streptogramin (iMLS), constitutive MLS (cMLS), and macrolide-streptogramin (MS) resistance phenotypes, were examined by a double-disk diffusion D test. The ermB gene was more frequent (35%; 97/274) than ermC (27%; 75/274) or ermA (21%; 58/274). All 97 ermB-positive isolates harbored Tn551 and IS1216V The majority (89/97) of ermB-positive isolates displayed the cMLS phenotype and carried mobile element structure (MES)-like structures, which has been previously reported in sequence type 59 (ST59) methicillin-resistant S. aureus (MRSA). The remaining 8 ermB-carrying isolates, belonging to ST7 (n = 4), ST5 (n = 3), and ST59 (n = 1), were sasK intact and did not carry MES-like structures. Unlike a MES-like structure that was located on the chromosome, the ermB elements on sasK-intact isolates were located on plasmids by S1 nuclease pulsed-field gel electrophoresis (PFGE) analysis and conjugation tests. Sequence data for the ermB-containing region (14,566 bp) from ST59 NTUH_3874 revealed that the best match was a Tn1546-like element in plasmid pMCCL2 DNA (GenBank accession number AP009486) of Macrococcus caseolyticus Tn1546 is recognized as an enterococcal transposon and was known from the vancomycin resistance gene cluster in vancomycin-resistant Enterococcus (VRE). So far, acquisitions of Tn1546 in S. aureus have occurred in clonal complex 5 (CC5) MRSA, but not in MSSA. This is the first report that MSSA harbors an Enterococcus faecium-originated ermB-positive Tn1546-like element located on a plasmid. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes

PubMed Central

Bevacqua, R. J.; Fernandez-Martin, R.; Canel, N. G.; Gibbons, A.; Texeira, D.; Lange, F.; Vans Landschoot, G.; Savy, V.; Briski, O.; Hiriart, M. I.; Grueso, E.; Ivics, Z.; Taboga, O.; Kues, W. A.; Ferraris, S.

2017-01-01

Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes. In the case of Tn5, the transposome complex of transposon nucleic acid and Tn5 protein was injected. In the case of SB, the supercoiled plasmids encoding a transposon and the SB transposase were co-injected. In vitro produced bovine zygotes were used to establish the cytoplasmic injection conditions. The in vitro cultured blastocysts were evaluated for reporter gene expression and genotyped. Subsequently, both transposon systems were injected in seasonally available ovine zygotes, employing transposons carrying the recombinant human factor IX driven by the beta-lactoglobulin promoter. The Tn5 approach did not result in transgenic lambs. In contrast, the Sleeping Beauty injection resulted in 2 lambs (29%) carrying the transgene. Both animals exhibited cellular mosaicism of the transgene. The extraembryonic tissues (placenta or umbilical cord) of three additional animals were also transgenic. These results show that transpositional transgenesis by cytoplasmic injection of SB transposon components can be applied for the production of transgenic lambs of pharmaceutical interest. PMID:28301581

Temperature-dependent instability of the cTnI subunit in NIST SRM2921 characterized by tryptic peptide mapping.

PubMed

van der Burgt, Yuri E M; Cobbaert, Christa M; Dalebout, Hans; Smit, Nico; Deelder, André M

2012-08-01

In this study temperature-dependent instability of the cTnI subunit of the three-protein complex NIST SRM2921 was demonstrated using a mass spectrometric tryptic peptide mapping approach. The results were compared to the cTnI subunit obtained as a protein standard from Calbiochem with identical amino acid sequence. Both the three-protein complex from NIST as well as the cTnI subunit were incubated at elevated temperatures and then evaluated with respect to the primary sequence. The corresponding peptide maps were analyzed using LC-MS/MS. From a Mascot database search in combination with "semiTrypsin" tolerance it was found that two peptide backbone cleavages had occurred in subunit cTnI in NIST SRM2921 material upon incubation at 37°C, namely between amino acids at 148/149 and 194/195. The Calbiochem standard did not show increased levels of "unexpected" peptides in tryptic peptide maps. One of the two peptide backbone cleavages could also be monitored using a "single-step" MALDI-MS approach, i.e. without the need for peptide separation. The amount of degradation appeared rather constant in replicate temperature-instability experiments. However, for accurate quantification internal labelled standards are needed. Copyright © 2012 Elsevier B.V. All rights reserved.

MnTnBuOE-2-PyP protects normal colorectal fibroblasts from radiation damage and simultaneously enhances radio/chemotherapeutic killing of colorectal cancer cells

PubMed Central

Kosmacek, Elizabeth A.; Chatterjee, Arpita; Tong, Qiang; Lin, Chi; Oberley, Rebecca E.

2016-01-01

Manganese porphyrins have been shown to be potent radioprotectors in a variety of cancer models. However, the mechanism as to how these porphyrins protect normal tissues from radiation damage still remains largely unknown. In the current study, we determine the effects of the manganese porphyrin, MnTnBuOE-2-PyP, on primary colorectal fibroblasts exposed to irradiation. We found that 2 Gy of radiation enhances the fibroblasts' ability to contract a collagen matrix, increases cell size and promotes cellular senesence. Treating fibroblasts with MnTnBuOE-2-PyP significantly inhibited radiation-induced collagen contraction, preserved cell morphology and also inhibited cellular senescence. We further showed that MnTnBuOE-2-PyP enhanced the overall viability of the fibroblasts following exposure to radiation but did not protect colorectal cancer cell viability. Specifically, MnTnBuOE-2-PyP in combination with irradiation, caused a significant decrease in tumor clonogenicity. Since locally advanced rectal cancers are treated with chemoradiation therapy followed by surgery and non-metastatic anal cancers are treated with chemoradiation therapy, we also investigated the effects of MnTnBuOE-2-PyP in combination with radiation, 5-fluorouracil with and without Mitomycin C. We found that MnTnBuOE-2-PyP in combination with Mitomycin C or 5-fluorouracil further enhances those compounds' ability to suppress tumor cell growth. When MnTnBuOE-2-PyP was combined with the two chemotherapeutics and radiation, we observed the greatest reduction in tumor cell growth. Therefore, these studies indicate that MnTnBuOE-2-PyP could be used as a potent radioprotector for normal tissue, while at the same time enhancing radiation and chemotherapy treatment for rectal and anal cancers. PMID:27119354

MnTnBuOE-2-PyP protects normal colorectal fibroblasts from radiation damage and simultaneously enhances radio/chemotherapeutic killing of colorectal cancer cells.

PubMed

Kosmacek, Elizabeth A; Chatterjee, Arpita; Tong, Qiang; Lin, Chi; Oberley-Deegan, Rebecca E

2016-06-07

Manganese porphyrins have been shown to be potent radioprotectors in a variety of cancer models. However, the mechanism as to how these porphyrins protect normal tissues from radiation damage still remains largely unknown. In the current study, we determine the effects of the manganese porphyrin, MnTnBuOE-2-PyP, on primary colorectal fibroblasts exposed to irradiation. We found that 2 Gy of radiation enhances the fibroblasts' ability to contract a collagen matrix, increases cell size and promotes cellular senesence. Treating fibroblasts with MnTnBuOE-2-PyP significantly inhibited radiation-induced collagen contraction, preserved cell morphology and also inhibited cellular senescence. We further showed that MnTnBuOE-2-PyP enhanced the overall viability of the fibroblasts following exposure to radiation but did not protect colorectal cancer cell viability. Specifically, MnTnBuOE-2-PyP in combination with irradiation, caused a significant decrease in tumor clonogenicity. Since locally advanced rectal cancers are treated with chemoradiation therapy followed by surgery and non-metastatic anal cancers are treated with chemoradiation therapy, we also investigated the effects of MnTnBuOE-2-PyP in combination with radiation, 5-fluorouracil with and without Mitomycin C. We found that MnTnBuOE-2-PyP in combination with Mitomycin C or 5-fluorouracil further enhances those compounds' ability to suppress tumor cell growth. When MnTnBuOE-2-PyP was combined with the two chemotherapeutics and radiation, we observed the greatest reduction in tumor cell growth. Therefore, these studies indicate that MnTnBuOE-2-PyP could be used as a potent radioprotector for normal tissue, while at the same time enhancing radiation and chemotherapy treatment for rectal and anal cancers.

Deformation of conjugate compliant fault zones induced by the 2013 Mw7.7 Baluchistan (Pakistan) earthquake

NASA Astrophysics Data System (ADS)

Dutta, Rishabh; Wang, Teng; Feng, Guangcai; Harrington, Jonathan; Vasyura-Bathke, Hannes; Jónsson, Sigurjón

2017-04-01

Strain localizations in compliant fault zones (with elastic moduli lower than the surrounding rocks) induced by nearby earthquakes have been detected using geodetic observations in a few cases in the past. Here we observe small-scale changes in interferometric Synthetic Aperture Radar (InSAR) measurements along multiple conjugate faults near the rupture of the 2013 Mw7.7 Baluchistan (Pakistan) earthquake. After removing the main coseismic deformation signal in the interferograms and correcting them for topography-related phase, we observe 2-3 cm signal along several conjugate faults that are 15-30 km from the mainshock fault rupture. These conjugate compliant faults have strikes of N30°E and N45°W. The sense of motion indicates left-lateral deformation across the N30°E faults and right-lateral deformation across the N45°W faults, which suggests the conjugate faults were subjected to extensional coseismic stresses along the WSW-ENE direction. The spacing between the different sets of faults is around 5 to 8 km. We explain the observed strain localizations as an elastic response of the compliant conjugate faults induced by the Baluchistan earthquake. Using 3D Finite Element models (FEM), we impose coseismic static displacements due to the earthquake along the boundaries of the FEM domain to reproduce the coseismic stress changes acting across the compliant faults. The InSAR measurements are used to constrain the geometry and rigidity variations of the compliant faults with respect to the surrounding rocks. The best fitting models show the compliant fault zones to have a width of 0.5 km to 2 km and a reduction of the shear modulus by a factor of 3 to 4. Our study yields similar values as were found for compliant fault zones near the 1992 Landers and the 1999 Hector Mine earthquakes in California, although here the strain localization is occurring on more complex conjugate sets of faults.

77 FR 8219 - Coastal Zone Management Program: Illinois

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-14

... DEPARTMENT OF COMMERCE National Oceanic and Atmospheric Administration Coastal Zone Management Program: Illinois AGENCY: Office of Ocean and Coastal Resource Management (OCRM), National Oceanic and... of Decision (ROD) for Federal Approval of the Illinois Coastal Management Program (ICMP). SUMMARY...

78 FR 58489 - Proposed Amendment of Class E Airspace; McMinnville, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-24

... County Memorial Airport, TN (Lat. 35[deg]41'55'' N., long. 85[deg]50'38'' W.) Columbia River Park... Flight Rules (IFR) operations at the airport. DATES: Comments must be received on or before November 8, 2013. ADDRESSES: Send comments on this rule to: U. S. Department of Transportation, Docket Operations...

14 CFR 77.28 - Military airport imaginary surfaces.

Code of Federal Regulations, 2010 CFR

2010-01-01

... (CONTINUED) AIRSPACE OBJECTS AFFECTING NAVIGABLE AIRSPACE Obstruction Standards § 77.28 Military airport.... These surfaces connect the primary surfaces, the first 200 feet of the clear zone surfaces, and the...

Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus.

PubMed

Kambarev, Stanimir; Pecorari, Frédéric; Corvec, Stéphane

2018-02-09

Streptococcus gallolyticus ssp. gallolyticus (Sgg) is a commensal bacterium and an opportunistic pathogen. In humans it has been clinically associated with the incidence of colorectal cancer (CRC) and epidemiologically recognized as an emerging cause of infective endocarditis (IE). The standard therapy of Sgg includes the administration of a penicillin in combination with an aminoglycoside. Even though penicillin-resistant isolates have still not been reported, epidemiological studies have shown that this microbe is a reservoir of multiple acquired genes, conferring resistance to tetracyclines, aminoglycosides, macrolides and glycopeptides. However, the underlying antibiotic resistance mobilome of Sgg remains poorly understood. To investigate the mobile genetic basis of antibiotic resistance in multiresistant clinical Sgg. Isolate NTS31106099 was recovered from a patient with IE and CRC at Nantes University Hospital, France and studied by Illumina WGS and comparative genomics. Molecular epidemiology of the identified mobile element(s) was performed using antibiotic susceptibility testing (AST), PCR, PFGE and WGS. Mobility was investigated by PCR and filter mating. Two novel conjugative transposons, Tn6263 and Tn6331, confer aminoglycoside/macrolide co-resistance in clinical Sgg. They display classical family Tn916/Tn1545 modular architecture and harbour an aph(3')-III→sat4→ant(6)-Ia→erm(B) multiresistance gene cluster, related to pRE25 of Enterococcus faecium. These and/or closely related elements are highly prevalent among genetically heterogeneous clinical isolates of Sgg. Previously unknown Tn916-like mobile genetic elements conferring aminoglycoside/macrolide co-resistance make Sgg, collectively with other gut Firmicutes such as enterococci and eubacteria, a potential laterally active reservoir of these antibiotic resistance determinants among the mammalian gastrointestinal microbiota. © The Author(s) 2018. Published by Oxford University Press on behalf

Interruption of capsule production in Streptococcus pneumonia serotype 3 by insertion of transposon Tn916.

PubMed Central

Watson, D A; Musher, D M

1990-01-01

Transposon Tn916 mutagenesis was used to produce mutant strains of Streptococcus pneumoniae serotype 3 that lacked only a polysaccharide capsule. Southern blotting, DNA-DNA hybridization, and immunochemical analyses demonstrated that the presence of a single copy of Tn916 was sufficient to produce unencapsulation. The 50% lethal dose for such mutants was greater than 5 x 10(7) CFU, as opposed to a 50% lethal dose of 1 CFU for wild-type strains. These experiments outline an effective method for targeting genes in S. pneumoniae by transposon interruption and provide molecular evidence to support the longstanding hypothesis that the capsule is the principal virulence factor in this pathogen. Images PMID:2167295

Characterization of Tn1546 in vancomycin-resistant Enterococcus faecium isolated from canine urinary tract infections: evidence of gene exchange between human and animal enterococci.

PubMed

Simjee, S; White, D G; McDermott, P F; Wagner, D D; Zervos, M J; Donabedian, S M; English, L L; Hayes, J R; Walker, R D

2002-12-01

Thirty-five enterococcal isolates were recovered from dogs diagnosed with urinary tract infections at the Michigan State University Veterinary Teaching Hospital over a 2-year period (1996 to 1998). Isolated species included Enterococcus faecium (n = 13), Enterococcus faecalis (n = 7), Enterococcus gallinarum (n = 11), and Enterococcus casseliflavus (n = 4). Antimicrobial susceptibility testing revealed several different resistance phenotypes, with the majority of the enterococcal isolates exhibiting resistance to three or more antibiotics. One E. faecium isolate, CVM1869, displayed high-level resistance to vancomycin (MIC > 32 micro g/ml) and gentamicin (MIC > 2,048 micro g/ml). Molecular analysis of this isolate revealed the presence of Tn1546 (vanA), responsible for high-level vancomycin resistance, and Tn5281 carrying aac6'-aph2", conferring high-level aminoglycoside resistance. Pulsed-field gel electrophoresis analysis revealed that CVM1869 was a canine E. faecium clone that had acquired Tn1546, perhaps from a human vancomycin-resistant E. faecium. Transposons Tn5281 and Tn1546 were located on two different conjugative plasmids. Sequence analysis revealed that in Tn1546, ORF1 had an 889-bp deletion and an IS1216V insertion at the 5' end and an IS1251 insertion between vanS and vanH. To date, this particular form of Tn1546 has only been described in human clinical vancomycin-resistant enterococcus isolates unique to the United States. Additionally, this is the first report of a vancomycin-resistant E. faecium isolated from a companion animal in the United States.

77 FR 27381 - Safety Zone; Newport High School Graduation Fireworks, Newport, OR

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-10

...-AA00 Safety Zone; Newport High School Graduation Fireworks, Newport, OR AGENCY: Coast Guard, DHS....1. 2. Add Sec. 165.T216 Safety Zone; Newport High School Graduation Fireworks Display; Newport, OR Sec. 165.T216 Safety Zone; Newport High School Graduation Fireworks Display; Newport, OR Location The...

78 FR 49254 - Foreign-Trade Zone 84-Houston, Texas; Application for Subzone; Toshiba International Corporation...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-13

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-77-2013] Foreign-Trade Zone 84--Houston, Texas; Application for Subzone; Toshiba International Corporation; Houston, Texas An application has been submitted..., requesting subzone status for the facilities of Toshiba International Corporation located in Houston, Texas...

Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation.

PubMed

Vedantam, Gayatri; Knopf, Sarah; Hecht, David W

2006-01-01

Tn5520 is the smallest known bacterial mobilizable transposon and was isolated from an antibiotic resistant Bacteroides fragilis clinical isolate. When a conjugation apparatus is provided in trans, Tn5520 is mobilized (transferred) efficiently within, and from, both Bacteroides spp. and Escherichia coli. Only two genes are present on Tn5520; one encodes an integrase, and the other a multifunctional mobilization (Mob) protein BmpH. BmpH is essential for Tn5520 mobility. The focus of this study was to identify the Tn5520 origin of conjugative transfer (oriT) and to study BmpH-oriT binding. We delimited the functional Tn5520 oriT to a 71 bp sequence upstream of the bmpH gene. A plasmid vector harbouring this minimal 71 bp oriT was mobilized at the same frequency as that of intact Tn5520. The minimal oriT contains one 17 bp inverted repeat (IR) sequence. We constructed and tested multiple IR mutants and showed that the IR was essential in its entirety for mobilization. A nick site sequence (5'-GCTAC-3') was also identified within the minimal oriT; this sequence resembled nick sites found in plasmids of Gram positive origin. We further showed that mutation of a highly conserved GC dinucleotide in the nick site sequence completely abolished mobilization. We also purified BmpH and showed that it specifically bound a Tn5520 oriT fragment in electrophoretic mobility shift assays. We also identified non-nick site sequences within the minimal oriT that were essential for mobilization. We hypothesize that transposon-based single Mob protein systems may contribute to efficient gene dissemination from Bacteroides spp., because fewer DNA processing proteins are required for relaxosome formation.

77 FR 35903 - Safety Zone, Atlantic Intracoastal Waterway; Emerald Isle, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... 1625-AA00 Safety Zone, Atlantic Intracoastal Waterway; Emerald Isle, NC AGENCY: Coast Guard, DHS... zone on the waters of the Atlantic Intracoastal Waterway at Emerald Isle, North Carolina. The safety... Intracoastal Waterway, mile 226, at Emerald Isle, North Carolina. [[Page 35904

Morphological and functional diversity of ectomycorrhizal fungi on Roan Mountain (NC/TN)

Treesearch

Claire Bird; Coleman McCleneghan

2005-01-01

A comparison of ectomycorrhizal morphotypes and hypogeous fungi (truffles and false-truffles) in northern hardwood and spruce-fir forests on Roan Mountain (NC/TN) was performed to increase our knowledge of the fungal communities in the Southern Appalachian high elevation forests. These forests are home to an endangered subspecies and mycophagist, the Carolina northern...

77 FR 38492 - Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-28

... 1625-AA00 Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY AGENCY: Coast Guard, DHS. ACTION... the Olcott fireworks on July 3, 2012. The safety zone is necessary to protect participants, spectators, and vessels from the hazards associated with a firework display. [[Page 38493

77 FR 30451 - Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-23

...-AA00 Safety Zone; Olcott Fireworks, Lake Ontario, Olcott, NY AGENCY: Coast Guard, DHS. ACTION: Notice... Ontario during the Olcott fireworks display. The safety zone established by this proposed rule is necessary to protect spectators, participants, and vessels from the hazards associated with firework display...

77 FR 44463 - Safety Zone, Atlantic Intracoastal Waterway; Emerald Isle, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-30

... 1625-AA00 Safety Zone, Atlantic Intracoastal Waterway; Emerald Isle, NC AGENCY: Coast Guard, DHS... waters of the Atlantic Intracoastal Waterway at Emerald Isle, North Carolina. The safety zone is... Bridge crossing the Atlantic Intracoastal Waterway, mile 226, at Emerald Isle, North Carolina. The safety...

77 FR 35857 - Safety Zone, Fireworks Display, Lake Superior; Duluth, MN

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... 1625-AA00 Safety Zone, Fireworks Display, Lake Superior; Duluth, MN AGENCY: Coast Guard, DHS. ACTION... of Lake Superior during the Duluth Fourth Fest fireworks display. This temporary safety zone is necessary to protect spectators and vessels from the hazards associated with fireworks displays. DATES: This...

77 FR 64906 - Safety Zone, Atlantic Intracoastal Waterway; Emerald Isle, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-24

... 1625-AA00 Safety Zone, Atlantic Intracoastal Waterway; Emerald Isle, NC AGENCY: Coast Guard, DHS... zone on the waters of the Atlantic Intracoastal Waterway at Emerald Isle, North Carolina. The safety... NC 58 Fixed Bridge crossing the Atlantic Intracoastal Waterway, mile 226, at Emerald Isle, North...

77 FR 57063 - Safety Zone, Atlantic Intracoastal Waterway; Emerald Isle, NC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-17

... 1625-AA00 Safety Zone, Atlantic Intracoastal Waterway; Emerald Isle, NC AGENCY: Coast Guard, DHS... zone on the waters of the Atlantic Intracoastal Waterway at Emerald Isle, North Carolina. The safety... NC 58 Fixed Bridge crossing the Atlantic Intracoastal Waterway, mile 226, at Emerald Isle, North...

Recruitment of CRISPR-Cas systems by Tn7-like transposons.

PubMed

Peters, Joseph E; Makarova, Kira S; Shmakov, Sergey; Koonin, Eugene V

2017-08-29

A survey of bacterial and archaeal genomes shows that many Tn7-like transposons contain minimal type I-F CRISPR-Cas systems that consist of fused cas8f and cas5f , cas7f , and cas6f genes and a short CRISPR array. Several small groups of Tn7-like transposons encompass similarly truncated type I-B CRISPR-Cas. This minimal gene complement of the transposon-associated CRISPR-Cas systems implies that they are competent for pre-CRISPR RNA (precrRNA) processing yielding mature crRNAs and target binding but not target cleavage that is required for interference. Phylogenetic analysis demonstrates that evolution of the CRISPR-Cas-containing transposons included a single, ancestral capture of a type I-F locus and two independent instances of type I-B loci capture. We show that the transposon-associated CRISPR arrays contain spacers homologous to plasmid and temperate phage sequences and, in some cases, chromosomal sequences adjacent to the transposon. We hypothesize that the transposon-encoded CRISPR-Cas systems generate displacement (R-loops) in the cognate DNA sites, targeting the transposon to these sites and thus facilitating their spread via plasmids and phages. These findings suggest the existence of RNA-guided transposition and fit the guns-for-hire concept whereby mobile genetic elements capture host defense systems and repurpose them for different stages in the life cycle of the element.

An efficient (t,n) threshold quantum secret sharing without entanglement

NASA Astrophysics Data System (ADS)

Qin, Huawang; Dai, Yuewei

2016-04-01

An efficient (t,n) threshold quantum secret sharing (QSS) scheme is proposed. In our scheme, the Hash function is used to check the eavesdropping, and no particles need to be published. So the utilization efficiency of the particles is real 100%. No entanglement is used in our scheme. The dealer uses the single particles to encode the secret information, and the participants get the secret through measuring the single particles. Compared to the existing schemes, our scheme is simpler and more efficient.

76 FR 70321 - Prevailing Rate Systems; Redefinition of the Northern Mississippi and Memphis, TN, Appropriated...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-14

... OFFICE OF PERSONNEL MANAGEMENT 5 CFR Part 532 RIN 3206-AM37 Prevailing Rate Systems; Redefinition... on a consensus recommendation of the Federal Prevailing Rate Advisory Committee (FPRAC) to best match... which OPM received no comments. The Federal Prevailing Rate Advisory Committee (FPRAC), the national...

Different Variations of Néel Temperature TN and Kondo Temperature TK in the Alloy System Ce(Ru1-xOsx)2Al10 under Uniaxial Pressure

NASA Astrophysics Data System (ADS)

Takeuchi, Takashi; Hayashi, Kyosuke; Umeo, Kazunori; Takabatake, Toshiro

2018-05-01

We report magnetic, transport, and specific-heat measurements for single crystals of the antiferromagnetic (AFM) Kondo semiconductor alloy series Ce(Ru1-xOsx)2Al10 (0 ≤ x ≤ 1), which crystallize into an orthorhombic structure. The specific-heat and resistivity data show that the isoelectronic substitution does not damage the hybridization gap or the AFM transition. The Kondo temperature TK increases linearly with x, whereas the Néel temperature TN exhibits a maximum value of 29.2 K for x = 0.71. Under increasing uniaxial pressure P || a, TN increases for x = 0 but decreases for x = 1, while TK increases in the entire range of x. Under P || b, in contrast, TN increases steadily in the whole range of x while TK remains unchanged for each x. The strongly anisotropic change in TN indicates the presence of another mechanism to enhance TN in this system in addition to the anisotropic hybridization of the 4f state with conduction bands.

Enhanced nitrogen removal from piggery wastewater with high NH4+ and low COD/TN ratio in a novel upflow microaerobic biofilm reactor.

PubMed

Meng, Jia; Li, Jiuling; Li, Jianzheng; Antwi, Philip; Deng, Kaiwen; Nan, Jun; Xu, Pianpian

2018-02-01

To enhance nutrient removal more cost-efficiently in microaerobic process treating piggery wastewater characterized by high ammonium (NH 4 + -N) and low chemical oxygen demand (COD) to total nitrogen (TN) ratio, a novel upflow microaerobic biofilm reactor (UMBR) was constructed and the efficiency in nutrient removal was evaluated with various influent COD/TN ratios and reflux ratios. The results showed that the biofilm on the carriers had increased the biomass in the UMBR and enhanced the enrichment of slow-growth-rate bacteria such as nitrifiers, denitrifiers and anammox bacteria. The packed bed allowed the microaerobic biofilm process perform well at a low reflux ratio of 35 with a NH 4 + -N and TN removal as high as 93.1% and 89.9%, respectively. Compared with the previously developed upflow microaerobic sludge reactor, the UMBR had not changed the dominant anammox approach to nitrogen removal, but was more cost-efficiently in treating organic wastewater with high NH 4 + -N and low COD/TN ratio. Copyright © 2017 Elsevier Ltd. All rights reserved.

78 FR 6257 - Proposed Amendment of Class C Airspace; Nashville International Airport, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-30

...-0031; Airspace Docket No. 12-AWA-7] RIN 2120-AA66 Proposed Amendment of Class C Airspace; Nashville... rulemaking (NPRM). SUMMARY: This action proposes to modify the Nashville International Airport, TN, Class C... around an airport that is now permanently closed. The FAA is proposing this action to return the Class C...

77 FR 49730 - Safety Zone; Antique Boat Show, Niagara River, Grand Island, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-17

...-AA00 Safety Zone; Antique Boat Show, Niagara River, Grand Island, NY AGENCY: Coast Guard, DHS. ACTION... during the Antique Boat Show powerboat races. This safety zone is necessary to protect spectators..., 2012, we published a notice of proposed rulemaking entitled Safety Zone; Antique Boat Show, Niagara...

77 FR 42179 - Safety Zone; Fireworks Display, Potomac River, Charles County, Newburg, MD

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-18

...]30[sec] W, located at Newburg in Charles County, Maryland (NAD 1983). The temporary safety zone will... 1625-AA00 Safety Zone; Fireworks Display, Potomac River, Charles County, Newburg, MD AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard will establish a safety zone upon...

77 FR 74777 - Safety Zones; Grain-Shipment Assistance Vessels; Columbia and Willamette Rivers

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-18

... 1625-AA00 Safety Zones; Grain-Shipment Assistance Vessels; Columbia and Willamette Rivers AGENCY: Coast... temporary safety zones around the following four vessels: the motor vessel Daniel Foss, IMO 7638454, the... Connor Foss, Official 1238813. These safety zones apply while these vessels are located on the waters of...

77 FR 25080 - Safety Zones; TriMet Bridge Project, Willamette River, Portland, OR

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-27

...-AA00 Safety Zones; TriMet Bridge Project, Willamette River, Portland, OR AGENCY: Coast Guard, DHS... trestles and construction cranes involved in the construction of the TriMet Bridge on the Willamette River... project. These safety zones replace the prior safety zones established for the TriMet Bridge construction...

77 FR 29901 - Safety Zone; Marysville Days Fireworks, St. Clair River, Marysville, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-21

...-AA00 Safety Zone; Marysville Days Fireworks, St. Clair River, Marysville, MI AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the St. Clair River, Marysville, Michigan. This zone is intended to restrict vessels from a portion of the St...

77 FR 37604 - Safety Zone; Fourth of July Fireworks, Berkeley Marina, Berkeley, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-22

...: The Coast Guard will enforce a 1,000 foot safety zone around the Berkeley Pier in position 37[deg]51... Zone; Fourth of July Fireworks, Berkeley Marina, Berkeley, CA AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast Guard will enforce the safety zone for the Berkeley...

77 FR 41686 - Safety Zone; Sheffield Lake Fireworks, Lake Erie, Sheffield Lake, OH

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-16

...-AA00 Safety Zone; Sheffield Lake Fireworks, Lake Erie, Sheffield Lake, OH AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on Lake Erie, Sheffield Lake, OH. This safety zone is intended to restrict vessels from a portion of Lake Erie...

Diagnostic value of analysis of H-FABP, NT-proBNP, and cTnI in heart function in children with congenital heart disease and pneumonia.

PubMed

Zhou, F-J; Zhou, C-Y; Tian, Y-J; Xiao, A-J; Li, P-L; Wang, Y-H; Jia, J-W

2014-01-01

To analyze the expression of heart-fatty acid-binding protein (H-FABP), N-terminal pro-brain natriuretic peptide (NT-proBNP), and cTnI in children with congenital heart disease (CHD) and pneumonia, and evaluate its diagnostic value in heart failure (HF). The levels of serum H-FABP, NT-proBNP, and cardiac troponin 1 (cTnI) were measured by immunoassays in 22 children with CHD, pneumonia, and HF (group I), 25 children with CHD and pneumonia (group II), and 25 healthy children without CHD or pneumonia (control group). The concentration and positive rate of serum H-FABP, NT-proBNP, and cTnI were significantly higher in group I than those in group II. Compared to control group, these indexes were increased in both group I and group II. There were statistical significant differences in the positive rate of NT-proBNP and cTnI but not H-FABP between groups of patients with different classes of heart function. The levels of H-FABP, NT-proBNP, and cTnI were correlated with heart function, and can be used for the diagnosis of early-stage HF in children with CHD.

77 FR 31493 - Safety Zones; Fourth of July Fireworks Displays Within the Captain of the Port Charleston Zone, SC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-29

... temporary safety zones during Fourth of July Fireworks Displays on certain navigable waterways in Hilton... below. 1. Hilton Head Island, South Carolina. All waters within a 500 yard radius around the barge from... each safety zone. All coordinates are North American Datum 1983. [[Page 31496

[Improvement of butanol production by Escherichia coli via Tn5 transposon mediated mutagenesis].

PubMed

Lin, Zhao; Dong, Hongjun; Li, Yin

2015-12-01

For engineering an efficient butanol-producing Escherichia coli strain, many efforts have been paid on the known genes or pathways based on current knowledge. However, many genes in the genome could also contribute to butanol production in an unexpected way. In this work, we used Tn5 transposon to construct a mutant library including 1 196 strains in a previously engineered butanol-producing E. coli strain. To screen the strains with improved titer of butanol production, we developed a high-throughput method for pyruvate detection based on dinitrophenylhydrazine reaction using 96-well microplate reader, because pyruvate is the precursor of butanol and its concentration is inversely correlated with butanol in the fermentation broth. Using this method, we successfully screened three mutants with increased butanol titer. The insertion sites of Tn5 transposon was in the ORFs of pykA, tdk, and cadC by inverse PCR and sequencing. These found genes would be efficient targets for further strain improvement. And the genome scanning strategy described here will be helpful for other microbial cell factory construction.

77 FR 58512 - Grant of Authority; Establishment of a Foreign-Trade Zone, Brunswick, ME

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-21

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1845] Grant of Authority; Establishment of a Foreign-Trade Zone, Brunswick, ME Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u), the Foreign-Trade Zones Board (the Board) adopts...

77 FR 52681 - Reorganization and Expansion of Foreign-Trade Zone 87 Lake Charles, LA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-30

... Foreign-Trade Zone 87 Lake Charles, LA Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u), the Foreign-Trade Zones Board (the Board) adopts the following Order: Whereas, the Lake Charles Harbor & Terminal District, grantee of Foreign-Trade Zone 87...

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT) Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China

PubMed Central

Xiong, Yao; Liu, Bian; Hao, Zilong; Tao, Wendan; Liu, Ming

2016-01-01

Background and Objective Elevated levels of high sensitivity cardiac troponin T (hs-cTnT) occur in a substantial proportion of patients with acute ischemic stroke (AIS) and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT) or prognosis in AIS patients with rheumatic heart disease (RHD) remains unclear. Methods Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels(≥14ng/L) and patients with normal hs-cTnT levels (<14ng/L). Results The final analysis involved 84 patients (31 males; mean age, 61.6±12.2years), of whom serum hs-cTnT levels were elevated in 58.3%. Renal impairment was independently associated with elevated hs-cTnT levels (OR 4.184, 95%CI 1.17 to 15.01, P = 0.028), and patients with elevated hs-cTnT levels were at significantly higher risk of HT, 3-month mortality and 3-month disability/mortality (all P≤0.029). After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels. Conclusion Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies. PMID:26849554

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT) Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China.

PubMed

Liu, Junfeng; Wang, Deren; Xiong, Yao; Liu, Bian; Hao, Zilong; Tao, Wendan; Liu, Ming

2016-01-01

Elevated levels of high sensitivity cardiac troponin T (hs-cTnT) occur in a substantial proportion of patients with acute ischemic stroke (AIS) and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT) or prognosis in AIS patients with rheumatic heart disease (RHD) remains unclear. Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within 1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels (≥14 ng/L) and patients with normal hs-cTnT levels (<14 ng/L). The final analysis involved 84 patients (31 males; mean age, 61.6±12.2 years), of whom serum hs-cTnT levels were elevated in 58.3%. Renal impairment was independently associated with elevated hs-cTnT levels (OR 4.184, 95%CI 1.17 to 15.01, P = 0.028), and patients with elevated hs-cTnT levels were at significantly higher risk of HT, 3-month mortality and 3-month disability/mortality (all P≤0.029). After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels. Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies.

An unusual Tn21-like transposon containing an ars operon is present in highly arsenic-resistant strains of the biomining bacterium Acidithiobacillus caldus.

PubMed

Tuffin, I Marla; de Groot, Peter; Deane, Shelly M; Rawlings, Douglas E

2005-09-01

A transposon, TnAtcArs, that carries a set of arsenic-resistance genes was isolated from a strain of the moderately thermophilic, sulfur-oxidizing, biomining bacterium Acidithiobacillus caldus. This strain originated from a commercial plant used for the bio-oxidation of gold-bearing arsenopyrite concentrates. Continuous selection for arsenic resistance over many years had made the bacterium resistant to high concentrations of arsenic. Sequence analysis indicated that TnAtcArs is 12 444 bp in length and has 40 bp terminal inverted repeat sequences and divergently transcribed resolvase and transposase genes that are related to the Tn21-transposon subfamily. A series of genes consisting of arsR, two tandem copies of arsA and arsD, two ORFs (7 and 8) and arsB is situated between the resolvase and transposase genes. Although some commercial strains of At. caldus contained the arsDA duplication, when transformed into Escherichia coli, the arsDA duplication was unstable and was frequently lost during cultivation or if a plasmid containing TnAtcArs was conjugated into a recipient strain. TnAtcArs conferred resistance to arsenite and arsenate upon E. coli cells. Deletion of one copy of arsDA had no noticeable effect on resistance to arsenite or arsenate in E. coli. ORFs 7 and 8 had clear sequence similarity to an NADH oxidase and a CBS-domain-containing protein, respectively, but their deletion did not affect resistance to arsenite or arsenate in E. coli. TnAtcArs was actively transposed in E. coli, but no increase in transposition frequency in the presence of arsenic was detected. Northern hybridization and reporter gene studies indicated that although ArsR regulated the 10 kb operon containing the arsenic-resistance genes in response to arsenic, ArsR had no effect on the regulation of genes associated with transposition activity.

78 FR 13857 - Foreign-Trade Zone 93-Raleigh-Durham, NC; Authorization of Production Activity; Revlon Consumer...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-01

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-77-2012] Foreign-Trade Zone 93--Raleigh-Durham, NC; Authorization of Production Activity; Revlon Consumer Products Corporation (Hair Coloring Products); Oxford, NC On October 10, 2012, Revlon Consumer Products Corporation, the operator of FTZ 93G, submitted a notification of proposed production...

9 CFR 77.7 - Accredited-free States or zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS TUBERCULOSIS Cattle... no evidence of the spread of tuberculosis, the State or zone may retain its accredited-free status... apply the herd test requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis...

9 CFR 77.22 - Accredited-free States or zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS TUBERCULOSIS Captive... tuberculosis, the State or zone may retain its accredited-free status. If two or more affected herds are... requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999...

9 CFR 77.22 - Accredited-free States or zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS TUBERCULOSIS Captive... tuberculosis, the State or zone may retain its accredited-free status. If two or more affected herds are... requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999...

9 CFR 77.22 - Accredited-free States or zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS TUBERCULOSIS Captive... tuberculosis, the State or zone may retain its accredited-free status. If two or more affected herds are... requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999...

9 CFR 77.22 - Accredited-free States or zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS TUBERCULOSIS Captive... tuberculosis, the State or zone may retain its accredited-free status. If two or more affected herds are... requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999...

9 CFR 77.22 - Accredited-free States or zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS TUBERCULOSIS Captive... tuberculosis, the State or zone may retain its accredited-free status. If two or more affected herds are... requirements contained in the “Uniform Methods and Rules—Bovine Tuberculosis Eradication” (January 22, 1999...

77 FR 15009 - Safety Zones; Sellwood Bridge Project, Willamette River; Portland, OR

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-14

...-AA00 Safety Zones; Sellwood Bridge Project, Willamette River; Portland, OR AGENCY: Coast Guard, DHS... zones to remain in effect throughout the duration of the construction and renewal of the Sellwood Bridge... safe distance from the construction area while transiting in the vicinity of the Sellwood Bridge...

77 FR 63289 - Foreign-Trade Zone 32-Miami, Florida; Application for Reorganization Under Alternative Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-16

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-51-2012] Foreign-Trade Zone 32--Miami, Florida... submitted to the Foreign-Trade Zones Board (the Board) by the Greater Miami Foreign-Trade Zone, Inc., grantee of FTZ 32, to amend its application to reorganize FTZ 32 zone under the alternative site framework...

Efficient transformation of sucrose into high pullulan concentrations by Aureobasidium melanogenum TN1-2 isolated from a natural honey.

PubMed

Jiang, Hong; Xue, Si-Jia; Li, Yan-Feng; Liu, Guang-Lei; Chi, Zhen-Ming; Hu, Zhong; Chi, Zhe

2018-08-15

A very high pullulan producing yeast-like fungus, Aureobasidium melanogenum TN1-2 isolated from a natural honey was found to be able to produce 97.0 g/L of pullulan from 140.0 g/L sucrose at a flask level while it could yield 114.0 g/L of pullulan within 134 h during a 10-liter fermentation, the yield was 0.81 g/g and the productivity was 0.86 g/L/h. The high ability to biosynthesize pullulan by this yeast-like fungal strain TN1-2 was related to high glucosyltransferase activity, high phosphofructo-2-kinase activity, high content of its cellular glycerol and low glucose repressor. The Mw of the produced pullulan was 1.42 × 10 5  g/mol. The low Mw may be due to the high α-amylase, glucoamylase and isopullulanase activities. The intracellular level of trehalose had no influence on high pullulan production by the yeast-like fungal strain TN1-2. Copyright © 2018. Published by Elsevier Ltd.

Letter from Brian Croft and Andrew Johnson, Tetra Tech EM, Inc., to Steve Spurlin, USEPA. Subject: Emergency Response Report, American Drum and Pallet Site, TN. EPA Contract Ep-W-05-054, TDD No. TTEMI-05-001-0040

EPA Pesticide Factsheets

Contains April l4, 2008 letter submitting the emergency response report for the American Drum and Pallet Site in Memphis, Shelby County, Tennessee. This report summarizes field activities conducted at the site from June 11, 2007 through January 18, 2008.

77 FR 29251 - Safety Zone; Carnival Fireworks Display, Nantasket Beach, Hull, MA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-17

...-AA00 Safety Zone; Carnival Fireworks Display, Nantasket Beach, Hull, MA AGENCY: Coast Guard, DHS... zone on the navigable waters off of Nantasket Beach in the vicinity of Hull, MA for a Carnival... spectators and vessels from the hazards associated with fireworks displays. Discussion of Proposed Rule Hull...

77 FR 20719 - Safety Zone; Matlacha Bridge Construction, Matlacha Pass, Matlacha, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-06

...-AA00 Safety Zone; Matlacha Bridge Construction, Matlacha Pass, Matlacha, FL AGENCY: Coast Guard, DHS... waters of Matlacha Pass in the vicinity of the Matlacha Bridge in Matlacha, Florida. The safety zone will be enforced during construction of the Matlacha Bridge from Monday, March 12, 2012 through Tuesday...

77 FR 12994 - Safety Zone for Margate Bridge, Intracoastal Waterway; Margate, NJ

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-05

...-AA00 Safety Zone for Margate Bridge, Intracoastal Waterway; Margate, NJ AGENCY: Coast Guard, DHS... Intracoastal Waterway near Margate, NJ. This safety zone is necessary to ensure safety while the Margate Bridge... this rule. It is impracticable to publish an NPRM because the Margate Bridge Company gave short notice...

77 FR 1870 - Safety Zone; Matlacha Bridge Construction, Matlacha Pass, Matlacha, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-01-12

...-AA00 Safety Zone; Matlacha Bridge Construction, Matlacha Pass, Matlacha, FL AGENCY: Coast Guard, DHS... waters of Matlacha Pass in the vicinity of the Matlacha Bridge in Matlacha, Florida. The safety zone will be enforced during construction of the Matlacha Bridge from Thursday, December 15, 2011 until Sunday...

77 FR 62440 - Safety Zone; Wounded Warriors Benefit, Lake Erie, Huron, OH

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-15

...-AA00 Safety Zone; Wounded Warriors Benefit, Lake Erie, Huron, OH AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the waters of Lake Erie, East Huron, Ohio. This regulation is intended to restrict vessels from portions of Lake Erie...

77 FR 47284 - Safety Zone; Dredge Arthur J, Lake Huron, Lakeport, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-08

...-AA00 Safety Zone; Dredge Arthur J, Lake Huron, Lakeport, MI AGENCY: Coast Guard, DHS. ACTION: Temporary... preparation for and salvage operations of the Arthur J. dredge vessel. This temporary safety zone is necessary... sinking of the dredge vessel Arthur J. precluded the Coast Guard from having sufficient time to publish an...

pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matsumoto, Yasuyuki; Zhang, Qing; Akita, Kaoru

2012-03-02

Highlights: Black-Right-Pointing-Pointer ppGalNAc-T13 was up-regulated in high metastatic sublines of Lewis lung cancer. Black-Right-Pointing-Pointer ppGalNAc-T13 expression enhanced cell invasion activity in low metastatic sublines. Black-Right-Pointing-Pointer Trimeric Tn antigen was induced in the transfectant cells of ppGalNAc-T13 cDNA. Black-Right-Pointing-Pointer A major protein carrying trimeric Tn structure was identified as Syndecan-1. Black-Right-Pointing-Pointer Silencing of ppGalNAc-T13 resulted in the reduction of invasion and of metastasis.. -- Abstract: In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. Thesemore » sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated

77 FR 6057 - Approval for Manufacturing Authority, Foreign-Trade Zone 22, Baxter Healthcare Corporation...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-07

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1810] Approval for Manufacturing Authority, Foreign-Trade Zone 22, Baxter Healthcare Corporation, (Pharmaceutical and Biological Intravenous Product Manufacturing), Chicago, IL Pursuant to its authority under the Foreign-Trade Zones Act of June 18...

Simultaneous denitrification and denitrifying phosphorus removal in a full-scale anoxic-oxic process without internal recycle treating low strength wastewater.

PubMed

Wang, Qibin; Chen, Qiuwen

2016-01-01

Performance of a full-scale anoxic-oxic activated sludge treatment plant (4.0×10(5) m(3)/day for the first-stage project) was followed during a year. The plant performed well for the removal of carbon, nitrogen and phosphorus in the process of treating domestic wastewater within a temperature range of 10.8°C to 30.5°C. Mass balance calculations indicated that COD utilization mainly occurred in the anoxic phase, accounting for 88.2% of total COD removal. Ammonia nitrogen removal occurred 13.71% in the anoxic zones and 78.77% in the aerobic zones. The contribution of anoxic zones to total nitrogen (TN) removal was 57.41%. Results indicated that nitrogen elimination in the oxic tanks was mainly contributed by simultaneous nitrification and denitrification (SND). The reduction of phosphorus mainly took place in the oxic zones, 61.46% of the total removal. Denitrifying phosphorus removal was achieved biologically by 11.29%. Practical experience proved that adaptability to gradually changing temperature of the microbial populations was important to maintain the plant overall stability. Sudden changes in temperature did not cause paralysis of the system just lower removal efficiency, which could be explained by functional redundancy of microorganisms that may compensate the adverse effects of temperature changes to a certain degree. Anoxic-oxic process without internal recycling has great potential to treat low strength wastewater (i.e., TN<35 mg/L) as well as reducing operation costs. Copyright © 2015. Published by Elsevier B.V.

Imaging Shallow Aquitard Breaches with P waves: Results from a Walk-away test and a Reflection Survey at two Sites in Memphis, Tennessee, USA

NASA Astrophysics Data System (ADS)

Ge, J.; Magnani, M.; Waldron, B. A.

2006-12-01

We present the results of two seismic reflection experiments conducted in the Great Memphis area in April and July 2006. The two experiments consisted in a walk-away test and in the acquisition of a 1 km seismic reflection profile. The acquisition of the seismic data is part of a larger effort aimed at imaging the lateral continuity of the Upper Claiborne confining clay that separates the Memphis aquifer, the region's primary drinking water source, from the upper unconfined aquifer and protects the drinking aquifer from exposure to potential contamination. During the walk-away test, four P-wave sources, a 7.5 kg sledge hammer, a 20 kg weight drop, a 12-gauge Buffalo gun, and a Minivibe source were tested at two sites with the goal of selecting the best P-wave seismic source and acquisition parameters for shallow reflection surveys. Boreholes nearby both sites encountered the Upper Claiborne unit at a depth ranging from 10 m to 40 m. One site is located within a 100-meter length of road median that can be considered an urban environment. The second site is located at Shelby Farms within the City of Memphis yet reflects a rural setting with minimal noise and no subsurface infrastructure. Performing identical walk-away tests at both sites, the results indicate that the energy source selection is site dependent. At the urban site, the energy generated by the weight drop source is more coherent and can be interpreted with more confidence on the recorded data. However the Shelby Farms site the 12-gauge shotgun produced the strongest recorded energy, the highest dominant frequency and the broadest frequency band (6- 110 Hz). Strong attenuations are observed at both sites with a much higher attenuation in the urban road median site, where the near surface materials consisted of gravels, sands, clays, and pebbles. For both sites, surface waves and refractions dominate the seismic recordings. Filtering and gain of the data revealed the presence of shallow reflections related

Tn5253 family integrative and conjugative elements carrying mef(I) and catQ determinants in Streptococcus pneumoniae and Streptococcus pyogenes.

PubMed

Mingoia, Marina; Morici, Eleonora; Morroni, Gianluca; Giovanetti, Eleonora; Del Grosso, Maria; Pantosti, Annalisa; Varaldo, Pietro E

2014-10-01

The linkage between the macrolide efflux gene mef(I) and the chloramphenicol inactivation gene catQ was first described in Streptococcus pneumoniae (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in Streptococcus pyogenes (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253 family integrative and conjugative elements (ICEs). The ICE from S. pneumoniae (ICESpn529IQ) was sequenced, whereas the ICEs from S. pyogenes (ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share the intSp 23FST81 integrase gene and an identical Tn916 fragment, whereas ICESpy005IQ has int5252 and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (intSp 23FST81 or int5252). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation, mef(I) and catQ were cotransferred at a high frequency from S. pyogenes Spy005 and at very low frequencies from S. pneumoniae Spn529 and S. pyogenes Spy029. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

77 FR 40521 - Security Zones, Seattle's Seafair Fleet Week Moving Vessels, Puget Sound, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

... 1625-AA87 Security Zones, Seattle's Seafair Fleet Week Moving Vessels, Puget Sound, WA AGENCY: Coast Guard, DHS. ACTION: Final rule. SUMMARY: The U.S. Coast Guard is establishing security zones around designated participating vessels that are not protected by the Naval Vessel Protection Zone in Seattle's...

77 FR 47429 - Agency Information Collection Activities; Petroleum Refineries in Foreign Trade Sub-zones

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-08

... Activities; Petroleum Refineries in Foreign Trade Sub-zones AGENCY: U.S. Customs and Border Protection (CBP... requirement concerning the Petroleum Refineries in Foreign Trade Sub-zones. This request for comment is being...: Title: Petroleum Refineries in Foreign Trade Sub-zones. OMB Number: 1651-0063. Form Number: None...

Linear optical pulse compression based on temporal zone plates.

PubMed

Li, Bo; Li, Ming; Lou, Shuqin; Azaña, José

2013-07-15

We propose and demonstrate time-domain equivalents of spatial zone plates, namely temporal zone plates, as alternatives to conventional time lenses. Both temporal intensity zone plates, based on intensity-only temporal modulation, and temporal phase zone plates, based on phase-only temporal modulation, are introduced and studied. Temporal zone plates do not exhibit the limiting tradeoff between temporal aperture and frequency bandwidth (temporal resolution) of conventional linear time lenses. As a result, these zone plates can be ideally designed to offer a time-bandwidth product (TBP) as large as desired, practically limited by the achievable temporal modulation bandwidth (limiting the temporal resolution) and the amount of dispersion needed in the target processing systems (limiting the temporal aperture). We numerically and experimentally demonstrate linear optical pulse compression by using temporal zone plates based on linear electro-optic temporal modulation followed by fiber-optics dispersion. In the pulse-compression experiment based on temporal phase zone plates, we achieve a resolution of ~25.5 ps over a temporal aperture of ~5.77 ns, representing an experimental TBP larger than 226 using a phase-modulation amplitude of only ~0.8π rad. We also numerically study the potential of these devices to achieve temporal imaging of optical waveforms and present a comparative analysis on the performance of different temporal intensity and phase zone plates.

77 FR 17012 - Foreign-Trade Zone 158-Vicksburg/Jackson, MS; Application for Manufacturing Authority; Morgan...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-23

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 17-2012] Foreign-Trade Zone 158... Furniture Covering Sets), Verona, MS An application has been submitted to the Foreign-Trade Zones Board (the Board) by the Greater Mississippi Foreign-Trade Zone, Inc., grantee of FTZ 158, requesting manufacturing...

Stability and normal zone propagation in YBCO CORC cables

DOE PAGES

Majoros, M.; Sumption, M. D.; Collings, E. W.; ...

2016-03-11

In this study, a two layer conductor on round core cable was tested for stability and normal zone propagation at 77 K in a liquid nitrogen bath. The cable was instrumented with voltage taps and wires on each strand over the cable’s central portion (i.e. excluding the end connections of the cable with the outside world). A heater was placed in the central zone on the surface of the cable, which allowed pulses of various powers and durations to be generated. Shrinking (recovering) and expanding (not recovering) normal zones have been detected, as well as stationary zones which were inmore » thermal equilibrium. Such stationary thermal equilibrium zones did not expand or contract, and hit a constant upper temperature while the heater current persisted; they are essentially a form of Stekly stability. Overall, the cable showed a high degree of stability. Notably, it was able to carry a current of 0.45I c cable with maximum temperature of 123 K for one minute without damage.« less

77 FR 38490 - Safety Zone; Mentor Harbor Yachting Club Fireworks, Lake Erie, Mentor, OH

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-28

...-AA00 Safety Zone; Mentor Harbor Yachting Club Fireworks, Lake Erie, Mentor, OH AGENCY: Coast Guard, DHS... Erie, Mentor, OH. This safety zone is intended to restrict vessels from a portion of Lake Erie during the Mentor Harbor Yachting Club fireworks display. This temporary safety zone is necessary to protect...

77 FR 49349 - Safety Zone; Chicago Air and Water Show, Lake Michigan, Chicago, IL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-16

... Zone; Chicago Air and Water Show, Lake Michigan, Chicago, IL AGENCY: Coast Guard, DHS. ACTION... Water Show safety zone on Lake Michigan near Lincoln Park. This action is necessary to accurately reflect the enforcement dates and times for this safety zone due to changes made in this year's air show...

77 FR 39638 - Safety Zone; Barbara Harder Wedding Fireworks, Lake Erie, Lake View, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-05

...-AA00 Safety Zone; Barbara Harder Wedding Fireworks, Lake Erie, Lake View, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on Lake Erie, Lake View, NY. This safety zone is intended to restrict vessels from a portion of Lake Erie...

77 FR 35850 - Safety Zone; F/V Deep Sea, Penn Cove, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-15

... 1625-AA00 Safety Zone; F/V Deep Sea, Penn Cove, WA AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a safety zone around the Fishing Vessel (F/V) Deep Sea... with the sunken F/V Deep Sea. B. Basis and Purpose On the evening of May 13, 2012, the F/V Deep Sea...

The L‐type Ca2+ channel facilitates abnormal metabolic activity in the cTnI‐G203S mouse model of hypertrophic cardiomyopathy

PubMed Central

Viola, Helena; Johnstone, Victoria; Cserne Szappanos, Henrietta; Richman, Tara; Tsoutsman, Tatiana; Filipovska, Aleksandra; Semsarian, Christopher

2016-01-01

Key points Genetic mutations in cardiac troponin I (cTnI) are associated with development of hypertrophic cardiomyopathy characterized by myocyte remodelling, disorganization of cytoskeletal proteins and altered energy metabolism.The L‐type Ca2+ channel is the main route for calcium influx and is crucial to cardiac excitation and contraction. The channel also regulates mitochondrial function in the heart by a functional communication between the channel and mitochondria via the cytoskeletal network.We find that L‐type Ca2+ channel kinetics are altered in cTnI‐G203S cardiac myocytes and that activation of the channel causes a significantly greater increase in mitochondrial membrane potential and metabolic activity in cTnI‐G203S cardiac myocytes.These responses occur as a result of impaired communication between the L‐type Ca2+ channel and cytoskeletal protein F‐actin, involving decreased movement of actin–myosin and block of the mitochondrial voltage‐dependent anion channel, resulting in a ‘hypermetabolic’ mitochondrial state.We propose that L‐type Ca2+ channel antagonists, such as diltiazem, might be effective in reducing the cardiomyopathy by normalizing mitochondrial metabolic activity. Abstract Genetic mutations in cardiac troponin I (cTnI) account for 5% of families with hypertrophic cardiomyopathy. Hypertrophic cardiomyopathy is associated with disorganization of cytoskeletal proteins and altered energy metabolism. The L‐type Ca2+ channel (ICa‐L) plays an important role in regulating mitochondrial function. This involves a functional communication between the channel and mitochondria via the cytoskeletal network. We investigate the role of ICa‐L in regulating mitochondrial function in 25‐ to 30‐week‐old cardiomyopathic mice expressing the human disease‐causing mutation Gly203Ser in cTnI (cTnI‐G203S). The inactivation rate of ICa‐L is significantly faster in cTnI‐G203S myocytes [cTnI‐G203S: τ1 = 40.68 ± 3.22, n

77 FR 39420 - Safety Zone; Bay Village Independence Day Fireworks, Lake Erie, Bay Village, OH

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-03

...-AA00 Safety Zone; Bay Village Independence Day Fireworks, Lake Erie, Bay Village, OH AGENCY: Coast... zone on Lake Erie, Bay Village, OH. This safety zone is intended to restrict vessels from a portion of Lake Erie during the Bay Village Independence Day Fireworks display. This temporary safety zone is...

77 FR 27021 - Foreign-Trade Zone 129-Bellingham, WA; Application for Reorganization Under Alternative Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-08

... FTZ 131 zone projects under FTZ 129. FTZ 130 was approved by the Board on September 4, 1986 (51 FR... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [(B-32-2012)] Foreign-Trade Zone 129--Bellingham... to the Foreign-Trade Zones (FTZ) Board (the Board) by the Port of Bellingham, grantee of FTZ 129...

77 FR 41373 - Reorganization and Expansion of Foreign-Trade Zone 99 Under Alternative Site Framework...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-13

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1839] Reorganization and Expansion of Foreign-Trade Zone 99 Under Alternative Site Framework; Wilmington, DE Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u), the Foreign-Trade Zones Board...

77 FR 44475 - Security Zones; Seattle's Seafair Fleet Week Moving Vessels, Puget Sound, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-30

...-AA87 Security Zones; Seattle's Seafair Fleet Week Moving Vessels, Puget Sound, WA AGENCY: Coast Guard... Seafair Fleet Week Moving Vessels Security Zones from 12:00 p.m. on July 31, 2012 through 5:00 p.m. on August 6, 2012. These security zones are necessary to help ensure the security of the vessels from...

On the almost everywhere convergence of the eigenfunction expansions from Liouville classes L_1^\\alpha ({T^N})

NASA Astrophysics Data System (ADS)

Ahmedov, Anvarjon; Materneh, Ehab; Zainuddin, Hishamuddin

2017-09-01

The relevance of waves in quantum mechanics naturally implies that the decomposition of arbitrary wave packets in terms of monochromatic waves plays an important role in applications of the theory. When eigenfunction expansions does not converge, then the expansions of the functions with certain smoothness should be considered. Such functions gained prominence primarily through their application in quantum mechanics. In this work we study the almost everywhere convergence of the eigenfunction expansions from Liouville classes L_p^α ({T^N}), related to the self-adjoint extension of the Laplace operator in torus TN . The sufficient conditions for summability is obtained using the modified Poisson formula. Isomorphism properties of the elliptic differential operators is applied in order to obtain estimation for the Fourier series of the functions from the classes of Liouville L_p^α .

Smartphone App Using Mindfulness Meditation for Women With Chronic Pelvic Pain (MEMPHIS): Protocol for a Randomized Feasibility Trial

PubMed Central

Newton, Sian; Kahan, Brennan C; Forbes, Gordon; Wright, Neil; Cantalapiedra Calvete, Clara; Gibson, Harry A L; Rogozinska, Ewelina; Rivas, Carol; Taylor, Stephanie J C; Birch, Judy; Dodds, Julie

2018-01-01

Background Female chronic pelvic pain (CPP) is defined as intermittent or constant pelvic or lower abdominal pain occurring in a woman for at least 6 months. Up to a quarter of women are estimated to be affected by CPP worldwide and it is responsible for one fifth of specialist gynecological referrals in the United Kingdom. Psychological interventions are commonly utilized. As waiting times and funding capacity impede access to face-to-face consultations, supported self-management (SSM) has emerged as a viable alternative. Mindfulness meditation is a potentially valuable SSM tool, and in the era of mobile technology, this can be delivered to the individual user via a smartphone app. Objective To assess the feasibility of conducting a trial of a mindfulness meditation intervention delivered by a mobile phone app for patients with CPP. The main feasibility objectives were to assess patient recruitment and app adherence, to obtain information to be used in the sample size estimate of a future trial, and to receive feedback on usability of the app. Methods Mindfulness Meditation for Women With Chronic Pelvic Pain (MEMPHIS) is a three-arm feasibility trial, that took place in two hospitals in the United Kingdom. Eligible participants were randomized in a 1:1:1 ratio to one of three treatment arms: (1) the intervention arm, consisting of a guided, spoken mindfulness meditation app; (2) an active control arm, consisting of a progressive muscle relaxation app; and (3) usual care (no app). Participants were followed-up for 6 months. Key feasibility outcomes included the time taken to recruit all patients for the study, adherence, and estimates to be used in the sample size calculation for a subsequent full-scale trial. Upon completion of the feasibility trial we will conduct focus groups to explore app usability and reasons for noncompliance. Results Recruitment for MEMPHIS took place between May 2016 and September 2016. The study was closed March 2017 and the report was

75 FR 28302 - American Food and Vending Spring Hill, TN; Notice of Negative Determination Regarding Application...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-20

... Spring Hill, TN; Notice of Negative Determination Regarding Application for Reconsideration By... negative determination applicable to workers and former workers at American Food and Vending, Spring Hill... eligible for TAA because they are service workers who provided services to General Motors, Spring Hill...

77 FR 38037 - Foreign-Trade Zone 70-Detroit, MI Application for Reorganization Under Alternative Site Framework

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-26

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-46-2012] Foreign-Trade Zone 70--Detroit, MI... Foreign-Trade Zones (FTZ) Board (the Board) by the Greater Detroit Foreign-Trade Zone, Inc., grantee of FTZ 70, requesting authority to reorganize the zone under the alternative site framework (ASF) adopted...

77 FR 43048 - Foreign-Trade Zone 32-Miami, FL; Application for Reorganization Under Alternative Site Framework

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-23

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-51-2012] Foreign-Trade Zone 32--Miami, FL... Foreign-Trade Zones (FTZ) Board (the Board) by the Greater Miami Foreign-Trade Zone, Inc., grantee of FTZ 32, requesting authority to reorganize the zone under the alternative site framework (ASF) adopted by...

77 FR 38482 - Safety Zone; Oswego Independence Celebration Fireworks, Oswego Harbor, Oswego, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-28

...-AA00 Safety Zone; Oswego Independence Celebration Fireworks, Oswego Harbor, Oswego, NY AGENCY: Coast... Oswego Harbor during the Oswego Independence Celebration Fireworks display. This temporary safety zone is necessary to protect spectators and vessels from the hazards associated with a fireworks display. DATES...

77 FR 34797 - Safety Zones; Recurring Events in Captain of the Port New York Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-12

... Safety Zone, 33 CFR 165.160(5.9). position 40[deg]45'56.9'' N, 074[deg]00'25.4'' W (NAD 1983..., 074[deg]04'29.6'' W (NAD 1983), approximately 800 yards southeast of the Midland Beach. Date: June 16...]30'52.1'' N, 074[deg]10'58.8'' W (NAD 1983), approximately 540 yards east of Wolfe's Pond Park...

77 FR 27116 - Safety Zone, Naval Helicopter Association Reunion Helicopter Demonstration, Elizabeth River...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-09

...-AA00 Safety Zone, Naval Helicopter Association Reunion Helicopter Demonstration, Elizabeth River... establishing a temporary safety zone on the Elizabeth River in the vicinity of Norfolk, VA to support the Naval... of life on navigable waters during the Naval Helicopter Association Reunion Helicopter Demonstration...

77 FR 43167 - Safety Zone; Electric Zoo Fireworks, East River, Randall's Island, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-24

... DEPARTMENT OF HOMELAND SECURITY Coast Guard 33 CFR Part 165 [Docket Number USCG-2012-0588] RIN 1625-AA00 Safety Zone; Electric Zoo Fireworks, East River, Randall's Island, NY AGENCY: Coast Guard... Zone; Electronic Zoo Fireworks, East River, Randall's Island, NY. (a) Regulated Area. The following...

A TALE of transposition: Tn3-like transposons play a major role in the spread of pathogenicity determinants of Xanthomonas citri and other xanthomonads.

PubMed

Ferreira, Rafael Marini; de Oliveira, Amanda Carolina P; Moreira, Leandro M; Belasque, José; Gourbeyre, Edith; Siguier, Patricia; Ferro, Maria Inês T; Ferro, Jesus A; Chandler, Michael; Varani, Alessandro M

2015-02-17

Members of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomonas citri plasmid pXAC64. These are flanked by short inverted repeats (IRs). The region was identified as a transposon, TnXax1, with typical Tn3 family features, including a transposase and two recombination genes. Two 14-bp palindromic sequences within a 193-bp potential resolution site occur between the recombination genes. Additional derivatives carrying different T3SEs and other passenger genes occur in different Xanthomonas species. The T3SEs include transcription activator-like effectors (TALEs). Certain TALEs are flanked by the same IRs as found in TnXax1 to form mobile insertion cassettes (MICs), suggesting that they may be transmitted horizontally. A significant number of MICs carrying other passenger genes (including a number of TALE genes) were also identified, flanked by the same TnXax1 IRs and delimited by 5-bp target site duplications. We conclude that a large fraction of T3SEs, including individual TALEs and potential pathogenicity determinants, have spread by transposition and that TnXax1, which exhibits all of the essential characteristics of a functional transposon, may be involved in driving MIC transposition. We also propose that TALE genes may diversify by fork slippage during the replicative Tn3 family transposition. These mechanisms may play a crucial role in the emergence of Xanthomonas pathogenicity. Xanthomonas genomes carry many insertion sequences (IS) and transposons, which play an important role in their evolution and architecture. This study reveals a key relationship between transposons and pathogenicity determinants in

77 FR 40511 - Safety Zone; GR Symphony Fireworks Display, Kalamazoo Lake, Saugatuck, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

...-AA00 Safety Zone; GR Symphony Fireworks Display, Kalamazoo Lake, Saugatuck, MI AGENCY: Coast Guard, DHS... Kalamazoo Lake during the GR Symphony Fireworks display. This temporary safety zone is necessary to protect spectators and vessels from the hazards associated with a fireworks display. DATES: This rule will be...

RADIOCARBON MEASUREMENT OF THE BIOGENIC CONTRIBUTION TO SUMMERTIME PM 2.5 AMBIENT AEROSOL IN NASHVILLE, TN

EPA Science Inventory

Radiocarbon (14C) measurements performed on PM-2.5 samples collected near Nashville, TN from June 21 to July 13, 1999, showed high levels of modern carbon, ranging from 56 to 80% of the total carbon in the samples. Radiocarbon measurements performed on dichloromethane extracts of...

77 FR 39406 - Safety Zone; Tom Graves Memorial Fireworks, Port Bay, Wolcott, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-03

... of any grant or loan recipients, and will not raise any novel legal or policy issues. The safety zone... through the safety zone when permitted by the Captain of the Port. 2. Impact on Small Entities The... the potential impact of regulations on small entities during rulemaking. The Coast Guard certifies...

77 FR 42647 - Safety Zone: San Diego Symphony POPS Fireworks; San Diego, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... 1625-AA00 Safety Zone: San Diego Symphony POPS Fireworks; San Diego, CA AGENCY: Coast Guard, DHS... waters of San Diego Bay in support of the San Diego Symphony POPS Fireworks. This safety zone is... David Varela, Waterways Management, U.S. Coast Guard Sector San Diego, Coast Guard; telephone 619-278...

Characterizing a middle to upper crustal shear zone: Microstructures, quartz c-axis fabrics, deformation temperatures and flow vorticity analysis of the northern Ailao Shan-Red River shear zone, China

NASA Astrophysics Data System (ADS)

Wu, Wenbin; Liu, Junlai; Zhang, Lisheng; Qi, Yinchuan; Ling, Chengyang

2017-05-01

Structural and microstructural characteristics, deformation temperatures and flow vorticities of the northern Ailao Shan (ALS) high-grade metamorphic belt provide significant information regarding the nature and tectonic evolution of the Ailao Shan-Red River (ASRR) shear zone. Mineral deformation mechanisms, quartz lattice-preferred orientation (LPO) patterns and the opening angles of quartz c-axis fabrics of samples from the Gasa section indicate that the northern ALS high-grade metamorphic belt has experienced progressive shear deformation. The early stage shearing is characterized by a gradual decrease of deformation temperatures from >650 °C at the northeastern unit to ca. 300 °C at the southwestern unit, that results in the formation of migmatites, mylonitic gneisses, thin bedded mylonites, mylonitic schists and phyllonites from the NE to SW across the strike of the shear zone. The late stage low-temperature (300-400 °C) shearing is superimposed on the early deformation throughout the belt with the formation of discrete, small-scale shear zones, especially in the thin-banded mylonitic rocks along both margins. The kinematic vorticity values estimated by rotated rigid porphyroclast method and oblique grain-shaped/quartz c-axis-fabric method imply that the general shear-dominated flow (0.49-0.77) progressively changed to a simple shear-dominated flow (0.77-1) toward the late stage of ductile deformation. The two stages of shearing are consistent with early shortening-dominated and late extrusion-controlled regional tectonic processes. The transition between them occurred at ca. 27 Ma in the ALS high-grade metamorphic belt along the ASRR shear zone. The large amount of strike-slip displacement along the ASRR shear zone is predominantly attributed to accelerated flow along the shear zone during the late extrusion-controlled tectonic process.

Asia-Pacific consensus statement on the optimal use of high-sensitivity troponin assays in acute coronary syndromes diagnosis: focus on hs-TnI

PubMed Central

Tan, Jack Wei Chieh; Lam, Carolyn S P; Kasim, Sazzli Shahlan; Aw, Tar Choon; Abanilla, Joel M; Chang, Wei-Ting; Dang, Van Phuoc; Iboleon-Dy, Maria; Mumpuni, Sari Sri; Phommintikul, Arintaya; Ta, Manh Cuong; Topipat, Punkiat; Yiu, Kai Hang; Cullen, Louise

2017-01-01

Objective High-sensitivity troponin (hs-Tn) assays need to be applied appropriately to improve diagnosis and patient outcomes in acute coronary syndromes (ACS). Methods Experts from Asia Pacific convened in 2015 to provide data-driven consensus-based, region-specific recommendations and develop an algorithm for the appropriate incorporation of this assay into the ACS assessment and treatment pathway. Results Nine recommendations were developed by the expert panel: (1) troponin is the preferred cardiac biomarker for diagnostic assessment of ACS and is indicated for patients with symptoms of possible ACS; (2) hs-Tn assays are recommended; (3) serial testing is required for all patients; (4) testing should be performed at presentation and 3 hours later; (5) gender-specific cut-off values should be used for hs-Tn I assays; (6) hs-Tn I level >10 times the upper limit of normal should be considered to ‘rule in’ a diagnosis of ACS; (7) dynamic change >50% in hs-Tn I level from presentation to 3-hour retest identifies patients at high risk for ACS; (8) where only point-of-care testing is available, patients with elevated readings should be considered at high risk, while patients with low/undetectable readings should be retested after 6 hours or sent for laboratory testing and (9) regular education on the appropriate use of troponin tests is essential. Conclusions We propose an algorithm that will potentially reduce delays in discharge by the accurate ‘rule out’ of non-ACS patients within 3 hours. Appropriate research should be undertaken to ensure the efficacy and safety of the algorithm in clinical practice, with the long-term goal of improvement of care of patients with ACS in Asia Pacific. PMID:28466882

77 FR 19573 - Safety Zone; Wedding Fireworks Display, Boston Inner Harbor, Boston, MA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-02

...-AA00 Safety Zone; Wedding Fireworks Display, Boston Inner Harbor, Boston, MA AGENCY: Coast Guard, DHS... zone on the navigable waters of the Boston Inner Harbor in the vicinity of Anthony's Pier 4, Boston, MA... Boston Inner Harbor in the vicinity of Anthony's Pier 4, Boston, MA. The Captain of the Port (COTP...

77 FR 28769 - Safety Zone; Fourth of July Fireworks, City of Antioch, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-16

... Zone; Fourth of July Fireworks, City of Antioch, CA AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast Guard will enforce the safety zone for the City of Antioch... 4, 2012 the loaded barge will transit from Fulton Shipyard Pier to the launch site off the City of...

77 FR 8805 - Foreign-Trade Zone 107-Polk County, IA; Application for Reorganization Under Alternative Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-15

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 10-2012] Foreign-Trade Zone 107--Polk County, IA; Application for Reorganization Under Alternative Site Framework An application has been submitted to the Foreign-Trade Zones (FTZ) Board (the Board) by the Iowa Foreign Trade Zone Corporation...

77 FR 23425 - Revisions of Boundaries, Regulations and Zoning Scheme for Florida Keys National Marine Sanctuary...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-19

... Zoning Scheme for Florida Keys National Marine Sanctuary; Revisions of Fish and Wildlife Service and... Boundaries, Regulations and Zoning Scheme for Florida Keys National Marine Sanctuary and Key West and Great... sanctuary) boundaries, regulations and zoning scheme. This review of existing regulations and marine zoning...

Heart-type fatty acid-binding protein (H-FABP) and highly sensitive troponin T (hsTnT) as markers of myocardial injury and cardiovascular events in elective percutaneous coronary intervention (PCI).

PubMed

Connolly, M; Shand, J; Kinnin, M; Menown, I; Kurth, M J; Lamont, J; Mc Eneaney, D

2018-01-01

Type 4a myocardial infarction (MI) occurs when myocardial injury is combined with either symptoms suggestive of myocardial ischaemia, new left bundle branch block, angiographic loss of patency of a major artery or imaging suggestive of new loss of myocardium. Myocardial injury is defined as a rise of >5 x 99th upper reference limit (URL) of 14 ng/l (i.e. >70 ng/l) for highly sensitive troponin T (hsTnT) at 6 h if hsTnT was normal at baseline or >20% rise from 0 to 6 h if hsTnT was >14 ng/l at baseline. To assess the prognostic value of biomarkers of myocardial injury following elective percutaneous coronary intervention (PCI). A cohort of 209 patients were included of whom 144 (68.9%) were male, mean age was 68.8 years, 28 (13.4%) were smokers, 31 (14.8%) were diabetic, 199 (95.2%) had hypercholesterolaemia and 138 (66.0%) had hypertension. We evaluated hsTnT, heart-type fatty acid-binding protein (H-FABP), troponin I (TnI), creatine kinase MB type (CKMB), myoglobin, glycogen phosphorylase BB (GPBB) and carbonic anhydrase III (CA III) at 0, 4, 6 and 24 h following elective PCI. Patients were followed up at 1 year to assess for major adverse clinical events (MACE). Myocardial injury was observed in 37 (17.7%) patients. Median hsTnT/H-FABP at 4 h were most predictive. MACE was noted in 6 (2.9%) patients, 3 had type 4a MI post PCI, P = 0.036. Median 4 h hsTnT/H-FABP were most predictive of myocardial injury following PCI. H-FABP and hsTnT were predictive of MACE. © The Author 2017. Published by Oxford University Press on behalf of the Association of Physicians. All rights reserved. For Permissions, please email: journals.permissions@oup.com

'Test n Treat (TnT)'- Rapid testing and same-day, on-site treatment to reduce rates of chlamydia in sexually active further education college students: study protocol for a cluster randomised feasibility trial.

PubMed

Kerry-Barnard, Sarah; Fleming, Charlotte; Reid, Fiona; Phillips, Rachel; Drennan, Vari M; Adams, Elisabeth J; Majewska, Wendy; Balendra, Anjella; Harding-Esch, Emma; Cousins, Emma; Tariq Sadiq, S; Oakeshott, Pippa

2018-06-05

Sexually active young people attending London further education (FE) colleges have high rates of chlamydia, but screening rates are low. We will conduct a cluster randomised feasibility trial of frequent, rapid, on-site chlamydia testing and same-day treatment (Test and Treat (TnT)) in six FE colleges (with parallel qualitative and economic assessments) to assess the feasibility of conducting a future trial to investigate if TnT reduces chlamydia rates. We will recruit 80 sexually active students aged 16-24 years from public areas at each of six colleges. All participants (total n = 480) will be asked to provide samples (urine for males, self-taken vaginal swabs for females) and complete questionnaires on sexual lifestyle and healthcare use at baseline and after 7 months. Participants will be informed that baseline samples will not be tested for 7 months and be advised to get screened separately. Colleges will be randomly allocated to the intervention (TnT) or the control group (no TnT). One and 4 months after recruitment, participants at each intervention college (n = 3) will be texted and invited for on-site chlamydia tests using the 90-min Cepheid GeneXpert system. Students with positive results will be asked to see a visiting nurse health adviser for same-day treatment and partner notification, (backed by genitourinary medicine follow-up). Participants in control colleges (n = 3) will receive 'thank you' texts 1 and 4 months after recruitment. Seven months after recruitment, participants from both groups will be invited to complete questionnaires and provide samples for TnT. All samples will be tested, and same-day treatment offered to students with positive results. Acceptability of TnT will be assessed by qualitative interviews of purposively sampled students (n = 30) and college staff (n = 12). We will collect data on costs of TnT and usual healthcare. Findings will provide key values to inform feasibility, sample size and timescales of a

77 FR 24177 - Approval for Manufacturing Authority; Foreign-Trade Zone 177; Hoosier Stamping & Mfg. Corp...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-23

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1822] Approval for Manufacturing Authority; Foreign-Trade Zone 177; Hoosier Stamping & Mfg. Corp. (Wheel Assemblies and Accessories); Chandler, IN Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U...

77 FR 52310 - Expansion of Foreign-Trade Zone 61, San Juan, Puerto Rico

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-29

... 61, San Juan, Puerto Rico Pursuant to its authority under the Foreign-Trade Zones Act of June 18... Order: Whereas, the Puerto Rico Trade and Export Company, grantee of Foreign-Trade Zone 61, submitted an application to the Board for authority to expand FTZ 61 to include a site in Aguadilla, Puerto Rico, adjacent...

77 FR 43048 - Foreign-Trade Zone 8-Toledo, OH; Application for Reorganization and Expansion Under Alternative...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-23

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [B-49-2012] Foreign-Trade Zone 8--Toledo, OH... submitted to the Foreign-Trade Zones (FTZ) Board (the Board) by the Toledo-Lucas County Port Authority, grantee of FTZ 8, requesting authority to reorganize and expand the zone under the alternative site...

Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

PubMed Central

Gunderson, Leonard L.; Moughan, Jennifer; Ajani, Jaffer A.; Pedersen, John E.; Winter, Kathryn A.; Benson, Al B.; Thomas, Charles R.; Mayer, Robert J.; Haddock, Michael G.; Rich, Tyvin A.; Willett, Christopher G.

2013-01-01

Purpose The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the log–rank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray’s test. Results Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories. PMID:24035327

77 FR 28770 - Safety Zone; Fourth of July Fireworks, City of Eureka, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-16

... Zone; Fourth of July Fireworks, City of Eureka, CA AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast Guard will enforce the safety zone for the City of Eureka Fourth... Island near the City of Eureka, CA at position 40[deg]48'29'' N, 124[deg]10'06'' W (NAD 83) where it will...

77 FR 60672 - Approval for Manufacturing Authority, Foreign-Trade Zone 99, Fisker Automotive, Inc., (Electric...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-04

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1860] Approval for Manufacturing Authority, Foreign-Trade Zone 99, Fisker Automotive, Inc., (Electric Passenger Vehicles), Wilmington, DE Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u...

77 FR 39404 - Safety Zone; Wicomico Community Fireworks Rain Date, Great Wicomico River, Mila, VA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-03

...-AA00 Safety Zone; Wicomico Community Fireworks Rain Date, Great Wicomico River, Mila, VA AGENCY: Coast... zone on the Great Wicomico River in the vicinity of Mila, VA in support of the Wicomico Community Rain... as follows: Sec. 165.T05-0425 Safety Zone; Wicomico Community Fireworks Rain Date, Great Wicomico...

77 FR 59891 - Reorganization of Foreign-Trade Zone 107 Under Alternative Site Framework, Polk County, IA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-01

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1858] Reorganization of Foreign-Trade Zone 107 Under Alternative Site Framework, Polk County, IA Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u), the Foreign-Trade Zones Board (the...

77 FR 34935 - Foreign-Trade Zone 161; Temporary/Interim Manufacturing Authority; Siemens Energy, Inc., (Wind...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-12

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket T-4-2012] Foreign-Trade Zone 161; Temporary/Interim Manufacturing Authority; Siemens Energy, Inc., (Wind Turbine Nacelles and Hubs); Notice of Approval On April 2, 2012, the Executive Secretary of the Foreign-Trade Zones (FTZ) Board filed an application submitted by the Board of County...

77 FR 10960 - Security Zone, East River and Bronx Kill; Randalls and Wards Islands, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-24

...-AA87 Security Zone, East River and Bronx Kill; Randalls and Wards Islands, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary security zone on the... security zone is necessary to ensure the safety of the President of the United States, members of his...

77 FR 49401 - Safety Zones; Revolution 3 Triathlon, Lake Erie, Sandusky Bay, Cedar Point, OH

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-16

...-AA00 Safety Zones; Revolution 3 Triathlon, Lake Erie, Sandusky Bay, Cedar Point, OH AGENCY: Coast Guard... permanent safety zones on Lake Erie near Sandusky, OH. This action is necessary to provide for the safety of... injuries or fatalities. The Captain of the Port Detroit proposes to establish this safety zone to protect...

77 FR 55777 - Security Zones; Dignitary Arrival/Departure and United Nations Meetings, New York, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-11

... 1625-AA87 Security Zones; Dignitary Arrival/Departure and United Nations Meetings, New York, NY AGENCY... a permanent security zone on the waters of the East River and Bronx Kill, in the vicinity of... security zone on the East River, New York; and clarify the enforcement times and locations of the security...

77 FR 40266 - Safety Zone; Conneaut 4th of July Festival, Lake Erie, Conneaut, OH

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-09

... 1625-AA00 Safety Zone; Conneaut 4th of July Festival, Lake Erie, Conneaut, OH AGENCY: Coast Guard, DHS... the Conneaut 4th of July Festival Fireworks display. This temporary safety zone is necessary to... vessels during the Conneaut 4th of July Festival Fireworks. This zone will be effective and enforced from...

77 FR 14700 - Safety Zones; Swim Around Charleston, Charleston, SC

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-13

... the Swim Around Charleston, a swimming race occurring on the Wando River, the Cooper River, Charleston... 23, 2012, the Swim Around Charleston is scheduled to take place on the Wando River, the Cooper River...-AA00 Safety Zones; Swim Around Charleston, Charleston, SC AGENCY: Coast Guard, DHS. ACTION: Notice of...

77 FR 45334 - Reorganization of Foreign-Trade Zone 18 Under Alternative Site Framework; San Jose, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-31

... Zone 18 Under Alternative Site Framework; San Jose, CA Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u), the Foreign-Trade Zones Board (the..., California, grantee of Foreign-Trade Zone 18, submitted an application to the Board (FTZ Docket 28-2012...

77 FR 49351 - Safety Zone; Port Huron Float-Down, St. Clair River, Port Huron, MI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-16

...-AA00 Safety Zone; Port Huron Float-Down, St. Clair River, Port Huron, MI AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on the St. Clair River, Port Huron, MI. This zone is intended to restrict vessels from a portion of the St. Clair...

77 FR 60899 - Safety Zone; Sea World San Diego Fireworks, Mission Bay; San Diego, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-05

... 1625-AA00 Safety Zone; Sea World San Diego Fireworks, Mission Bay; San Diego, CA AGENCY: Coast Guard... navigable waters of Mission Bay in support of the Sea World San Diego Fireworks. This safety zone is... zones (33 U.S.C 1221 et seq.). Sea World is sponsoring the Sea World Fireworks, which will include a...

77 FR 42640 - Safety Zone; Can-Am Festival Fireworks, Black River Bay, Sackets Harbor, NY

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

...-AA00 Safety Zone; Can-Am Festival Fireworks, Black River Bay, Sackets Harbor, NY AGENCY: Coast Guard... Black River Bay during the Can-Am Festival Fireworks display. This temporary safety zone is necessary to... Can-Am Festival Fireworks. This zone will be effective and enforced from 9:15 p.m. until 10:45 p.m. on...

77 FR 9879 - Safety Zone; Lake Pontchartrain, New Orleans, LA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-21

...-AA00 Safety Zone; Lake Pontchartrain, New Orleans, LA AGENCY: Coast Guard, DHS. ACTION: Notice of... of the South shores of Lake Pontchartrain adjacent to the East bank of the Lakefront Airport runways... Blue Angels Air Show, to take place over the waters of Lake Pontchartrain. The Blue Angels Air Show is...

Troponin C Mutations Partially Stabilize the Active State of Regulated Actin and Fully Stabilize the Active State When Paired with Δ14 TnT.

PubMed

Baxley, Tamatha; Johnson, Dylan; Pinto, Jose R; Chalovich, Joseph M

2017-06-13

Striated muscle contraction is regulated by the actin-associated proteins tropomyosin and troponin. The extent of activation of myosin ATPase activity is lowest in the absence of both Ca 2+ and activating cross-bridges (i.e., S1-ADP or rigor S1). Binding of activating species of myosin to actin at a saturating Ca 2+ concentration stabilizes the most active state (M state) of the actin-tropomyosin-troponin complex (regulated actin). Ca 2+ binding alone produces partial stabilization of the active state. The extent of stabilization at a saturating Ca 2+ concentration depends on the isoform of the troponin subunits, the phosphorylation state of troponin, and, in the case of cardiac muscle, the presence of hypertrophic cardiomyopathy-producing mutants of troponin T and troponin I. Cardiac dysfunction is also associated with mutations of troponin C (TnC). Troponin C mutants A8V, C84Y, and D145E increase the Ca 2+ sensitivity of ATPase activity. We show that these mutants change the distribution of regulated actin states. The A8V and C84Y TnC mutants decreased the inactive B state distribution slightly at low Ca 2+ concentrations, but the D145E mutants had no effect on that state. All TnC mutants increased the level of the active M state compared to that of the wild type, at a saturating Ca 2+ concentration. Troponin complexes that contained two mutations that stabilize the active M state, A8V TnC and Δ14 TnT, appeared to be completely in the active state in the presence of only Ca 2+ . Because Ca 2+ gives full activation, in this situation, troponin must be capable of positioning tropomyosin in the active M state without the need for rigor myosin binding.

Abrasive wear of Hilong BoTN hardfacings

NASA Astrophysics Data System (ADS)

Fedorova, L.; Fedorov, S.; Sadovnikov, A.; Ivanova, Y.; Voronina, M.

2018-02-01

The spread of steels, which are used to produce locks of steel drill pipes, adversely affects their wear resistance, which, in combination with low hardness of HV 2400 ... 2800 MPa as well as of the thread of screw, results in low wear resistance and the need for their reconstruction at the pipe control shop. An efficient way of improving the quality of drill pipe jonts is to hard-face them by the outside diameter with wear-resistant materials (hardbanding). One of the companies engaged in the development of hardfacing materials and hardbanding is Hilong (China) with weld seams of the brand BoTn. According to the results of the studies the following conclusion can be made: hardfacing increases the durability of the hardware, contributing to an increase in wear resistance of locks of DP under the conditions of abrasive action of aggressive geological formations; the usage of DP without wear-resistant weld seams is impermissible, because their further operation, as part of the drill-stem, can lead to emergency consequences; application of the pipes with the hardfacing collars together with the collars without hardfacing, due to varying degree of wear of jonts in the drill-stem, is also impermissible.

9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2010 CFR

2010-01-01

... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only...

9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2011 CFR

2011-01-01

... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only...

9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2013 CFR

2013-01-01

... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only...

9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2012 CFR

2012-01-01

... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only...

78 FR 54168 - Special Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-03

... local regulation will not have a significant economic impact on a substantial number of small entities... Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary special local...

Implications for future activity of Grímsvötn volcano, Iceland, from compositional time series of historical tephra

NASA Astrophysics Data System (ADS)

Carpentier, Marion; Sigmarsson, Olgeir; Larsen, Gudrun

2014-05-01

The nature of future eruptions of active volcanoes is hard to predict. Improved understanding of the past volcanic activity is probably the best way to infer future eruptive scenarios. The most active volcano in Iceland, Grímsvötn, last erupted in 2011 with consequences for habitants living close to the volcano and aviation in the North-Atlantic. In an effort to better understand the magmatic system of the volcano, we have investigated the compositions of 23 selected tephra layers representing the last 8 centuries of volcanic activity at Grímsvötn. The tephra was collected in the ablation area of outlet glaciers from Vatnajökull ice cap. The ice-conserved tephra are less prone to alteration than those exposed in soil sections. Major element analyses are indistinguishable and of quartz-normative tholeiite composition, and Sr and Nd isotope ratios are constant. In contrast, both trace element concentrations (Th range from 0.875 ppm to 1.37 ppm and Ni from 28.5 ppm to 56.6 ppm) in the basalts and Pb isotopes show small but significant variations. The high-precision analyses of Pb isotope ratios allow the identification of tephra samples (3 in total) with more radiogenic ratios than the bulk of the samples. The tephra of constant isotope ratios show linear increase in incompatible element concentrations with time. The rate of increasing concentrations permits exploring possible future scenarios assuming that the magmatic system beneath the volcano follows the established historical evolution. Assuming similar future behaviour of the magma system beneath Grímsvötn volcano, the linear increase in e.g. Th concentration suggests that the volcano is likely to principally produce basalts for the next 500-1000 years. Evolution of water concentration will most likely follow those of incompatible elements with consequent increases in explosiveness of future Grímsvötn eruptions.

9 CFR 77.25 - Interstate movement from modified accredited advanced States and zones.

Code of Federal Regulations, 2014 CFR

2014-01-01

... ANIMAL PRODUCTS TUBERCULOSIS Captive Cervids § 77.25 Interstate movement from modified accredited..., and that are not known to be infected with or exposed to tuberculosis, may be moved interstate only in...

77 FR 16661 - Tuberculosis in Cattle and Bison; State and Zone Designations; NM; Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-22

...-0124] Tuberculosis in Cattle and Bison; State and Zone Designations; NM; Correction AGENCY: Animal and... in the regulatory text of an interim rule that amended the bovine tuberculosis regulations by establishing two separate zones with different tuberculosis risk classifications for the State of New Mexico...

77 FR 65623 - Security Zones; USCGC WILLIAM FLORES Commissioning Ceremony, Ybor Channel; Tampa, FL

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-30

...-AA87 Security Zones; USCGC WILLIAM FLORES Commissioning Ceremony, Ybor Channel; Tampa, FL AGENCY: Coast... zone on the waters of Ybor Channel in Tampa, Florida around the USCGC WILLIAM FLORES immediately before... FLORES, official parties, dignitaries, the public, and surrounding waterways from terrorist acts...

77 FR 61721 - Atlantic Ocean off Wallops Island and Chincoteague Inlet, VA; Danger Zone

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-11

... classes of orbital rockets. This amendment increases the permanent danger zone to a 30 nautical mile sector and is necessary to protect the public from hazards associated with rocket-launching operations... danger zone to accommodate larger classes of orbital rockets. This amendment increases the permanent...

TnSeq of Mycobacterium tuberculosis clinical isolates reveals strain-specific antibiotic liabilities

PubMed Central

Carey, Allison F.; Rock, Jeremy M.; Krieger, Inna V.; Gagneux, Sebastien; Sacchettini, James C.; Fortune, Sarah M.

2018-01-01

Once considered a phenotypically monomorphic bacterium, there is a growing body of work demonstrating heterogeneity among Mycobacterium tuberculosis (Mtb) strains in clinically relevant characteristics, including virulence and response to antibiotics. However, the genetic and molecular basis for most phenotypic differences among Mtb strains remains unknown. To investigate the basis of strain variation in Mtb, we performed genome-wide transposon mutagenesis coupled with next-generation sequencing (TnSeq) for a panel of Mtb clinical isolates and the reference strain H37Rv to compare genetic requirements for in vitro growth across these strains. We developed an analytic approach to identify quantitative differences in genetic requirements between these genetically diverse strains, which vary in genomic structure and gene content. Using this methodology, we found differences between strains in their requirements for genes involved in fundamental cellular processes, including redox homeostasis and central carbon metabolism. Among the genes with differential requirements were katG, which encodes the activator of the first-line antitubercular agent isoniazid, and glcB, which encodes malate synthase, the target of a novel small-molecule inhibitor. Differences among strains in their requirement for katG and glcB predicted differences in their response to these antimicrobial agents. Importantly, these strain-specific differences in antibiotic response could not be predicted by genetic variants identified through whole genome sequencing or by gene expression analysis. Our results provide novel insight into the basis of variation among Mtb strains and demonstrate that TnSeq is a scalable method to predict clinically important phenotypic differences among Mtb strains. PMID:29505613

A role for Tn6029 in the evolution of the complex antibiotic resistance gene loci in genomic island 3 in enteroaggregative hemorrhagic Escherichia coli O104:H4.

PubMed

Roy Chowdhury, Piklu; Charles, Ian G; Djordjevic, Steven P

2015-01-01

In enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104 the complex antibiotic resistance gene loci (CRL) found in the region of divergence 1 (RD1) within E. coli genomic island 3 (GI3) contains blaTEM-1, strAB, sul2, tet(A)A, and dfrA7 genes encoding resistance to ampicillin, streptomycin, sulfamethoxazole, tetracycline and trimethoprim respectively. The precise arrangement of antibiotic resistance genes and the role of mobile elements that drove the evolutionary events and created the CRL have not been investigated. We used a combination of bioinformatics and iterative BLASTn searches to determine the micro-evolutionary events that likely led to the formation of the CRL in GI3 using the closed genome sequences of EAHEC O104:H4 strains 2011C-3493 and 2009EL-2050 and high quality draft genomes of EAHEC E. coli O104:H4 isolates from sporadic cases not associated with the initial outbreak. Our analyses indicate that the CRL in GI3 evolved from a progenitor structure that contained an In2-derived class 1 integron in a Tn21/Tn1721 hybrid backbone. Within the hybrid backbone, a Tn6029-family transposon, identified here as Tn6029C abuts the sul1 gene in the 3'-Conserved Segment (-CS) of a class 1 integron generating a unique molecular signature that has only previously been observed in pASL01a, a small plasmid found in commensal E. coli in West Africa. From this common progenitor, independent IS26-mediated events created two novel transposons identified here as Tn6029D and Tn6222 in 2011C-3493 and 2009EL-2050 respectively. Analysis of RD1 within GI3 reveals IS26 has played a crucial role in the assembly of regions within the CRL.

77 FR 62494 - Proposed Information Collection; Comment Request; Evaluations of Coastal Zone Management Act...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-15

... Collection; Comment Request; Evaluations of Coastal Zone Management Act Programs--State Coastal Management... request is for a new information collection. The Coastal Zone Management Act of 1972, as amended (CZMA; 16 U.S.C. 1451 et seq.) requires that state coastal management programs and national estuarine research...

77 FR 40800 - Safety Zone: Sacramento River Closure for Aerial Cable Installation, Sacramento, CA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-11

...-AA00 Safety Zone: Sacramento River Closure for Aerial Cable Installation, Sacramento, CA AGENCY: Coast... zone in the navigable waters of the Sacramento River near Sherman Island, CA in support of the...; Sacramento River Closure for Aerial Cable Installation, Sacramento, CA. (a) Location. This temporary safety...

77 FR 28253 - Safety Zone; America's Cup World Series, East Passage, Narragansett Bay, RI

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-14

...-AA00 Safety Zone; America's Cup World Series, East Passage, Narragansett Bay, RI AGENCY: Coast Guard... navigable waters of the East Passage, Narragansett Bay, Rhode Island, during the America's Cup World Series... rulemaking (NPRM) entitled ``Safety Zones; America's Cup World Series, East Passage, Narragansett Bay, RI...