Sample records for zoogloea ramigera identification

  1. Influence of pH and Metal Cations on Aggregative Growth of Non-Slime-forming Strains of Zoogloea ramigera

    PubMed Central

    Angelbeck, Donald I.; Kirsch, Edwin J.

    1969-01-01

    Aggregative growth of non-slime-forming strains of Zoogloea ramigera was induced by growing the organisms at a depressed pH. Calcium and magnesium ion was found to reverse aggregative growth of the organisms. Conversely, aggregation was stimulated when the available inorganic cation concentration of the growth medium was lowered by the use of a chelating agent. The aggregative effects of pH depression or cation depletion and the dispersal effects of cation supplementation were observed only during cellular growth. The data suggest that aggregate formation of non-slime-forming strains of Z. ramigera may be related to the calcium or magnesium metabolism of the organisms, or both. Images PMID:4976326

  2. Molecular analysis of microflora associated with dentoalveolar abscesses.

    PubMed Central

    Dymock, D; Weightman, A J; Scully, C; Wade, W G

    1996-01-01

    The microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16S rRNA genes were randomly amplified by means of conserved eubacterial primers and cloned. Restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16S rRNA from the cultured bacteria was used to detect putative unculturable bacteria. Clones representative of five predominant groups of uncultured organisms were sequenced. Two were identified as Porphyromonas gingivalis and Prevotella oris, and one was found to be closely related to Peptostreptococcus micros. The remaining two clones did not correspond to known, previously sequenced organisms. One was related to Zoogloea ramigera, a species of aerobic waterborne organisms, while the other was distantly related to the genus Prevotella. This study has demonstrated the possibility of the characterization of microflora associated with human infection by molecular methods without the inherent biases of culture. PMID:8904410

  3. Isolation and characterization of heterotrophic bacteria able to grow aerobically with quaternary ammonium alcohols as sole source of carbon and nitrogen.

    PubMed

    Kaech, Andres; Vallotton, Nathalie; Egli, Thomas

    2005-04-01

    The quaternary ammonium alcohols (QAAs) 2,3-dihydroxypropyl-trimethyl-ammonium (TM), dimethyl-diethanol-ammonium (DM) and methyl-triethanol-ammonium (MM) are hydrolysis products of their parent esterquat surfactants, which are widely used as softeners in fabric care. We isolated several bacteria growing with QAAs as the sole source of carbon and nitrogen. The strains were compared with a previously isolated TM-degrading bacterium, which was identified as a representative of the species Pseudomonas putida (Syst. Appl. Microbiol. 24 (2001) 252). Two bacteria were isolated with DM, referred to as strains DM 1 and DM 2, respectively. Based on 16S-rDNA analysis, they provided 97% (DM 1) and 98% (DM 2) identities to the closest related strain Zoogloea ramigera Itzigsohn 1868AL. Both strains were long, slim, motile rods but only DM 1 showed the floc forming activity, which is typical for representatives of the genus Zoogloea. Using MM we isolated a Gram-negative, non-motile rod referred to as strain MM 1. The 16S-rDNA sequence of the isolated bacterium revealed 94% identities (best match) to Rhodobacter sphaeroides only. The strains MM 1 and DM 1 exclusively grew with the QAA which was used for their isolation. DM 2 was also utilizing TM as sole source of carbon and nitrogen. However, all of the isolated bacteria were growing with the natural and structurally related compound choline.

  4. Crystal structure and biochemical characterization of beta-keto thiolase B from polyhydroxyalkanoate-producing bacterium Ralstonia eutropha H16

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kim, Eun-Jung; Son, Hyeoncheol Francis; Kim, Sangwoo

    Highlights: • We determined a crystal structure of β-keto thiolase from Ralstonia eutropha H16 (ReBktB). • Distinct substrate binding mode ReBktB was elucidated. • Enzymatic kinetic parameters of ReBktB were revealed. - Abstract: ReBktB is a β-keto thiolase from Ralstonia eutropha H16 that catalyzes condensation reactions between acetyl-CoA with acyl-CoA molecules that contains different numbers of carbon atoms, such as acetyl-CoA, propionyl-CoA, and butyryl-CoA, to produce valuable bioproducts, such as polyhydroxybutyrate, polyhydroxybutyrate-hydroxyvalerate, and hexanoate. We solved a crystal structure of ReBktB at 2.3 Å, and the overall structure has a similar fold to that of type II biosynthetic thiolases, suchmore » as PhbA from Zoogloea ramigera (ZrPhbA). The superposition of this structure with that of ZrPhbA complexed with CoA revealed the residues that comprise the catalytic and substrate binding sites of ReBktB. The catalytic site of ReBktB contains three conserved residues, Cys90, His350, and Cys380, which may function as a covalent nucleophile, a general base, and second nucleophile, respectively. For substrate binding, ReBktB stabilized the ADP moiety of CoA in a distinct way compared to ZrPhbA with His219, Arg221, and Asp228 residues, whereas the stabilization of β-mercaptoethyamine and pantothenic acid moieties of CoA was quite similar between these two enzymes. Kinetic study of ReBktB revealed that K{sub m}, V{sub max}, and K{sub cat} values of 11.58 μM, 1.5 μmol/min, and 102.18 s{sup −1}, respectively, and the catalytic and substrate binding sites of ReBktB were further confirmed by site-directed mutagenesis experiments.« less

  5. Assessment of bacterial and structural dynamics in aerobic granular biofilms

    PubMed Central

    Weissbrodt, David G.; Neu, Thomas R.; Kuhlicke, Ute; Rappaz, Yoan; Holliger, Christof

    2013-01-01

    Aerobic granular sludge (AGS) is based on self-granulated flocs forming mobile biofilms with a gel-like consistence. Bacterial and structural dynamics from flocs to granules were followed in anaerobic-aerobic sequencing batch reactors (SBR) fed with synthetic wastewater, namely a bubble column (BC-SBR) operated under wash-out conditions for fast granulation, and two stirred-tank enrichments of Accumulibacter (PAO-SBR) and Competibacter (GAO-SBR) operated at steady-state. In the BC-SBR, granules formed within 2 weeks by swelling of Zoogloea colonies around flocs, developing subsequently smooth zoogloeal biofilms. However, Zoogloea predominance (37–79%) led to deteriorated nutrient removal during the first months of reactor operation. Upon maturation, improved nitrification (80–100%), nitrogen removal (43–83%), and high but unstable dephosphatation (75–100%) were obtained. Proliferation of dense clusters of nitrifiers, Accumulibacter, and Competibacter from granule cores outwards resulted in heterogeneous bioaggregates, inside which only low abundance Zoogloea (<5%) were detected in biofilm interstices. The presence of different extracellular glycoconjugates detected by fluorescence lectin-binding analysis showed the complex nature of the intracellular matrix of these granules. In the PAO-SBR, granulation occurred within two months with abundant and active Accumulibacter populations (56 ± 10%) that were selected under full anaerobic uptake of volatile fatty acids and that aggregated as dense clusters within heterogeneous granules. Flocs self-granulated in the GAO-SBR after 480 days during a period of over-aeration caused by biofilm growth on the oxygen sensor. Granules were dominated by heterogeneous clusters of Competibacter (37 ± 11%). Zoogloea were never abundant in biomass of both PAO- and GAO-SBRs. This study showed that Zoogloea, Accumulibacter, and Competibacter affiliates can form granules, and that the granulation mechanisms rely on the dominant population involved. PMID:23847600

  6. Nitrogen Removal Characteristics of a Newly Isolated Indigenous Aerobic Denitrifier from Oligotrophic Drinking Water Reservoir, Zoogloea sp. N299.

    PubMed

    Huang, Ting-Lin; Zhou, Shi-Lei; Zhang, Hai-Han; Bai, Shi-Yuan; He, Xiu-Xiu; Yang, Xiao

    2015-05-04

    Nitrogen is considered to be one of the most widespread pollutants leading to eutrophication of freshwater ecosystems, especially in drinking water reservoirs. In this study, an oligotrophic aerobic denitrifier was isolated from drinking water reservoir sediment. Nitrogen removal performance was explored. The strain was identified by 16S rRNA gene sequence analysis as Zoogloea sp. N299. This species exhibits a periplasmic nitrate reductase gene (napA). Its specific growth rate was 0.22 h-1. Obvious denitrification and perfect nitrogen removal performances occurred when cultured in nitrate and nitrite mediums, at rates of 75.53%±1.69% and 58.65%±0.61%, respectively. The ammonia removal rate reached 44.12%±1.61% in ammonia medium. Zoogloea sp. N299 was inoculated into sterilized and unsterilized reservoir source waters with a dissolved oxygen level of 5-9 mg/L, pH 8-9, and C/N 1.14:1. The total nitrogen removal rate reached 46.41%±3.17% (sterilized) and 44.88%±4.31% (unsterilized). The cell optical density suggested the strain could survive in oligotrophic drinking water reservoir water conditions and perform nitrogen removal. Sodium acetate was the most favorable carbon source for nitrogen removal by strain N299 (p<0.05). High C/N was beneficial for nitrate reduction (p<0.05). The nitrate removal efficiencies showed no significant differences among the tested inoculums dosage (p>0.05). Furthermore, strain N299 could efficiently remove nitrate at neutral and slightly alkaline and low temperature conditions. These results, therefore, demonstrate that Zoogloea sp. N299 has high removal characteristics, and can be used as a nitrogen removal microbial inoculum with simultaneous aerobic nitrification and denitrification in a micro-polluted reservoir water ecosystem.

  7. Effect of temperature and cycle length on microbial competition in PHB-producing sequencing batch reactor.

    PubMed

    Jiang, Yang; Marang, Leonie; Kleerebezem, Robbert; Muyzer, Gerard; van Loosdrecht, Mark C M

    2011-05-01

    The impact of temperature and cycle length on microbial competition between polyhydroxybutyrate (PHB)-producing populations enriched in feast-famine sequencing batch reactors (SBRs) was investigated at temperatures of 20 °C and 30 °C, and in a cycle length range of 1-18 h. In this study, the microbial community structure of the PHB-producing enrichments was found to be strongly dependent on temperature, but not on cycle length. Zoogloea and Plasticicumulans acidivorans dominated the SBRs operated at 20 °C and 30 °C, respectively. Both enrichments accumulated PHB more than 75% of cell dry weight. Short-term temperature change experiments revealed that P. acidivorans was more temperature sensitive as compared with Zoogloea. This is particularly true for the PHB degradation, resulting in incomplete PHB degradation in P. acidivorans at 20 °C. Incomplete PHB degradation limited biomass growth and allowed Zoogloea to outcompete P. acidivorans. The PHB content at the end of the feast phase correlated well with the cycle length at a constant solid retention time (SRT). These results suggest that to establish enrichment with the capacity to store a high fraction of PHB, the number of cycles per SRT should be minimized independent of the temperature.

  8. Nitrogen Removal Characteristics of a Newly Isolated Indigenous Aerobic Denitrifier from Oligotrophic Drinking Water Reservoir, Zoogloea sp. N299

    PubMed Central

    Huang, Ting-Lin; Zhou, Shi-Lei; Zhang, Hai-Han; Bai, Shi-Yuan; He, Xiu-Xiu; Yang, Xiao

    2015-01-01

    Nitrogen is considered to be one of the most widespread pollutants leading to eutrophication of freshwater ecosystems, especially in drinking water reservoirs. In this study, an oligotrophic aerobic denitrifier was isolated from drinking water reservoir sediment. Nitrogen removal performance was explored. The strain was identified by 16S rRNA gene sequence analysis as Zoogloea sp. N299. This species exhibits a periplasmic nitrate reductase gene (napA). Its specific growth rate was 0.22 h−1. Obvious denitrification and perfect nitrogen removal performances occurred when cultured in nitrate and nitrite mediums, at rates of 75.53% ± 1.69% and 58.65% ± 0.61%, respectively. The ammonia removal rate reached 44.12% ± 1.61% in ammonia medium. Zoogloea sp. N299 was inoculated into sterilized and unsterilized reservoir source waters with a dissolved oxygen level of 5–9 mg/L, pH 8–9, and C/N 1.14:1. The total nitrogen removal rate reached 46.41% ± 3.17% (sterilized) and 44.88% ± 4.31% (unsterilized). The cell optical density suggested the strain could survive in oligotrophic drinking water reservoir water conditions and perform nitrogen removal. Sodium acetate was the most favorable carbon source for nitrogen removal by strain N299 (p < 0.05). High C/N was beneficial for nitrate reduction (p < 0.05). The nitrate removal efficiencies showed no significant differences among the tested inoculums dosage (p > 0.05). Furthermore, strain N299 could efficiently remove nitrate at neutral and slightly alkaline and low temperature conditions. These results, therefore, demonstrate that Zoogloea sp. N299 has high removal characteristics, and can be used as a nitrogen removal microbial inoculum with simultaneous aerobic nitrification and denitrification in a micro-polluted reservoir water ecosystem. PMID:25946341

  9. Microbial Ecology of Activated Sludge

    PubMed Central

    Dias, F. F.; Bhat, J. V.

    1964-01-01

    Over 300 bacterial strains were isolated from seven samples of activated sludge by plating on sewage agar. Gram-negative bacteria of the genera Zoogloea and Comamonas predominated. Many isolates (51%) showed sudanophilic inclusions of poly-β-hydroxybutyric acid, whereas 34% accumulated iodophilic material on media containing starch. A large number required either vitamins or amino acids, or both, for growth. None of the isolates tested for their ability to bring about changes in autoclaved sewage produced an effluent comparable in quality to the activated sludge control, although the Zoogloea did produce activated sludgelike flocs. A study of 150 bacterial strains isolated from raw sewage revealed that they differed from the sludge isolates in several respects. Coliforms, which constitute nearly a quarter of the sewage isolates, were rarely encountered in sludge. PMID:14215970

  10. In situ profiling of microbial communities in full-scale aerobic sequencing batch reactors treating winery waste in australia.

    PubMed

    McIlroy, Simon J; Speirs, Lachlan B M; Tucci, Joseph; Seviour, Robert J

    2011-10-15

    On-site aerobic sequencing batch reactor (SBR) treatment plants are implemented in many Australian wineries to treat the large volumes of associated wastewater they generate. Yet very little is known about their microbiology. This paper represents the first attempt to analyze the communities of three such systems sampled during both vintage and nonvintage operational periods using molecular methods. Alphaproteobacterial tetrad forming organisms (TFO) related to members of the genus Defluviicoccus and Amaricoccus dominated all three systems in both operational periods. Candidatus 'Alysiosphaera europaea' and Zoogloea were codominant in two communities. Production of high levels of exocellular capsular material by Zoogloea and Amaricoccus is thought to explain the poor settleability of solids in one of these plants. The dominance of these organisms is thought to result from the high COD to N/P ratios that characterize winery wastes, and it is suggested that manipulating this ratio with nutrient dosing may help control the problems they cause.

  11. α- and β-Proteobacteria Control the Consumption and Release of Amino Acids on Lake Snow Aggregates

    PubMed Central

    Schweitzer, Bernhard; Huber, Ingrid; Amann, Rudolf; Ludwig, Wolfgang; Simon, Meinhard

    2001-01-01

    We analyzed the composition of aggregate (lake snow)-associated bacterial communities in Lake Constance from 1994 until 1996 between a depth of 25 m and the sediment surface at 110 m by fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes of various specificity. In addition, we experimentally examined the turnover of dissolved amino acids and carbohydrates together with the microbial colonization of aggregates formed in rolling tanks in the lab. Generally, between 40 and more than 80% of the microbes enumerated by DAPI staining (4′,6′-diamidino-2-phenylindole) were detected as Bacteria by the probe EUB338. At a depth of 25 m, 10.5% ± 7.9% and 14.2% ± 10.2% of the DAPI cell counts were detected by probes specific for α- and β-Proteobacteria. These proportions increased to 12.0% ± 3.3% and 54.0% ± 5.9% at a depth of 50 m but decreased again at the sediment surface at 110 m to 2.7% ± 1.4% and 41.1% ± 8.4%, indicating a clear dominance of β-Proteobacteria at depths of 50 and 110 m, where aggregates have an age of 3 to 5 and 8 to 11 days, respectively. From 50 m to the sediment surface, cells detected by a Cytophaga/Flavobacteria-specific probe (CF319a) comprised increasing proportions up to 18% of the DAPI cell counts. γ-Proteobacteria always comprised minor proportions of the aggregate-associated bacterial community. Using only two probes highly specific for clusters of bacteria closely related to Sphingomonas species and Brevundimonas diminuta, we identified between 16 and 60% of the α-Proteobacteria. In addition, with three probes highly specific for close relatives of the β-Proteobacteria Duganella zoogloeoides (formerly Zoogloea ramigera), Acidovorax facilis, and Hydrogenophaga palleroni, bacteria common in activated sludge, 42 to 70% of the β-Proteobacteria were identified. In the early phase (<20 h) of 11 of the 15 experimental incubations of aggregates, dissolved amino acids were consumed by the aggregate-associated bacteria from the surrounding water. This stage was followed by a period of 1 to 3 days during which dissolved amino acids were released into the surrounding water, paralleled by an increasing dominance of β-Proteobacteria. Hence, our results show that lake snow aggregates are inhabited by a community dominated by a limited number of α- and β-Proteobacteria, which undergo a distinct succession. They successively decompose the amino acids bound in the aggregates and release substantial amounts into the surrounding water during aging and sinking. PMID:11157226

  12. Aerobic Biodegradation Characteristic of Different Water-Soluble Azo Dyes.

    PubMed

    Sheng, Shixiong; Liu, Bo; Hou, Xiangyu; Wu, Bing; Yao, Fang; Ding, Xinchun; Huang, Lin

    2017-12-26

    This study investigated the biodegradation performance and characteristics of Sudan I and Acid Orange 7 (AO7) to improve the biological dye removal efficiency in wastewater and optimize the treatment process. The dyes with different water-solubility and similar molecular structure were biologically treated under aerobic condition in parallel continuous-flow mixed stirred reactors. The biophase analysis using microscopic examination suggested that the removal process of the two azo dyes is different. Removal of Sudan I was through biosorption, since it easily assembled and adsorbed on the surface of zoogloea due to its insolubility, while AO7 was biodegraded incompletely and bioconverted, the AO7 molecule was decomposed to benzene series and inorganic ions, since it could reach the interior area of zoogloea due to the low oxidation-reduction potential conditions and corresponding anaerobic microorganisms. The transformation of NH₃-N, SO₄ 2- together with the presence of tryptophan-like components confirm that AO7 can be decomposed to non-toxic products in an aerobic bioreactor. This study provides a theoretical basis for the use of biosorption or biodegradation mechanisms for the treatment of different azo dyes in wastewater.

  13. Bacterial Selection during the Formation of Early-Stage Aerobic Granules in Wastewater Treatment Systems Operated Under Wash-Out Dynamics

    PubMed Central

    Weissbrodt, David G.; Lochmatter, Samuel; Ebrahimi, Sirous; Rossi, Pierre; Maillard, Julien; Holliger, Christof

    2012-01-01

    Aerobic granular sludge is attractive for high-rate biological wastewater treatment. Biomass wash-out conditions stimulate the formation of aerobic granules. Deteriorated performances in biomass settling and nutrient removal during start-up have however often been reported. The effect of wash-out dynamics was investigated on bacterial selection, biomass settling behavior, and metabolic activities during the formation of early-stage granules from activated sludge of two wastewater treatment plants (WWTP) over start-up periods of maximum 60 days. Five bubble-column sequencing batch reactors were operated with feast-famine regimes consisting of rapid pulse or slow anaerobic feeding followed by aerobic starvation. Slow-settling fluffy granules were formed when an insufficient superficial air velocity (SAV; 1.8 cm s−1) was applied, when the inoculation sludge was taken from a WWTP removing organic matter only, or when reactors were operated at 30°C. Fast-settling dense granules were obtained with 4.0 cm s−1 SAV, or when the inoculation sludge was taken from a WWTP removing all nutrients biologically. However, only carbon was aerobically removed during start-up. Fluffy granules and dense granules were displaying distinct predominant phylotypes, namely filamentous Burkholderiales affiliates and Zoogloea relatives, respectively. The latter were predominant in dense granules independently from the feeding regime. A combination of insufficient solid retention time and of leakage of acetate into the aeration phase during intensive biomass wash-out was the cause for the proliferation of Zoogloea spp. in dense granules, and for the deterioration of BNR performances. It is however not certain that Zoogloea-like organisms are essential in granule formation. Optimal operation conditions should be elucidated for maintaining a balance between organisms with granulation propensity and nutrient removing organisms in order to form granules with BNR activities in short start-up periods. PMID:22993513

  14. Bacterial Selection during the Formation of Early-Stage Aerobic Granules in Wastewater Treatment Systems Operated Under Wash-Out Dynamics.

    PubMed

    Weissbrodt, David G; Lochmatter, Samuel; Ebrahimi, Sirous; Rossi, Pierre; Maillard, Julien; Holliger, Christof

    2012-01-01

    Aerobic granular sludge is attractive for high-rate biological wastewater treatment. Biomass wash-out conditions stimulate the formation of aerobic granules. Deteriorated performances in biomass settling and nutrient removal during start-up have however often been reported. The effect of wash-out dynamics was investigated on bacterial selection, biomass settling behavior, and metabolic activities during the formation of early-stage granules from activated sludge of two wastewater treatment plants (WWTP) over start-up periods of maximum 60 days. Five bubble-column sequencing batch reactors were operated with feast-famine regimes consisting of rapid pulse or slow anaerobic feeding followed by aerobic starvation. Slow-settling fluffy granules were formed when an insufficient superficial air velocity (SAV; 1.8 cm s(-1)) was applied, when the inoculation sludge was taken from a WWTP removing organic matter only, or when reactors were operated at 30°C. Fast-settling dense granules were obtained with 4.0 cm s(-1) SAV, or when the inoculation sludge was taken from a WWTP removing all nutrients biologically. However, only carbon was aerobically removed during start-up. Fluffy granules and dense granules were displaying distinct predominant phylotypes, namely filamentous Burkholderiales affiliates and Zoogloea relatives, respectively. The latter were predominant in dense granules independently from the feeding regime. A combination of insufficient solid retention time and of leakage of acetate into the aeration phase during intensive biomass wash-out was the cause for the proliferation of Zoogloea spp. in dense granules, and for the deterioration of BNR performances. It is however not certain that Zoogloea-like organisms are essential in granule formation. Optimal operation conditions should be elucidated for maintaining a balance between organisms with granulation propensity and nutrient removing organisms in order to form granules with BNR activities in short start-up periods.

  15. Aerobic Biodegradation Characteristic of Different Water-Soluble Azo Dyes

    PubMed Central

    Sheng, Shixiong; Liu, Bo; Hou, Xiangyu; Wu, Bing; Yao, Fang; Ding, Xinchun; Huang, Lin

    2017-01-01

    This study investigated the biodegradation performance and characteristics of Sudan I and Acid Orange 7 (AO7) to improve the biological dye removal efficiency in wastewater and optimize the treatment process. The dyes with different water-solubility and similar molecular structure were biologically treated under aerobic condition in parallel continuous-flow mixed stirred reactors. The biophase analysis using microscopic examination suggested that the removal process of the two azo dyes is different. Removal of Sudan I was through biosorption, since it easily assembled and adsorbed on the surface of zoogloea due to its insolubility, while AO7 was biodegraded incompletely and bioconverted, the AO7 molecule was decomposed to benzene series and inorganic ions, since it could reach the interior area of zoogloea due to the low oxidation-reduction potential conditions and corresponding anaerobic microorganisms. The transformation of NH3-N, SO42− together with the presence of tryptophan-like components confirm that AO7 can be decomposed to non-toxic products in an aerobic bioreactor. This study provides a theoretical basis for the use of biosorption or biodegradation mechanisms for the treatment of different azo dyes in wastewater. PMID:29278390

  16. Anaerobic mineralization of quaternary carbon atoms: isolation of denitrifying bacteria on pivalic acid (2,2-dimethylpropionic acid).

    PubMed

    Probian, Christina; Wülfing, Annika; Harder, Jens

    2003-03-01

    The degradability of pivalic acid was established by the isolation of several facultative denitrifying strains belonging to Zoogloea resiniphila, to Thauera and Herbaspirillum, and to Comamonadaceae, related to [Aquaspirillum] and Acidovorax, and of a nitrate-reducing bacterium affiliated with Moraxella osloensis. Pivalic acid was completely mineralized to carbon dioxide. The catabolic pathways may involve an oxidation to dimethylmalonate or a carbon skeleton rearrangement, a putative 2,2-dimethylpropionyl coenzyme A mutase.

  17. Bacterial community dynamics in long-term operation of a pilot plant using aerobic granular sludge to treat pig slurry.

    PubMed

    Fra-Vázquez, A; Morales, N; Figueroa, M; Val Del Río, A; Regueiro, L; Campos, J L; Mosquera-Corral, A

    2016-09-01

    Aerobic granular sludge represents an interesting approach for simultaneous organic matter and nitrogen removal in wastewater treatment plants. However, the information about microbial communities in aerobic granular systems dealing with industrial wastewater like pig slurry is limited. Herein, bacterial diversity and dynamics were assessed in a pilot scale plant using aerobic granular sludge for organic matter and nitrogen elimination from swine slurry during more than 300 days. Results indicated that bacterial composition evolved throughout the operational period from flocculent activated sludge, used as inoculum, to mature aerobic granules. Bacterial diversity increased at the beginning of the granulation process and then declined due to the application of transient organic matter and nitrogen loads. The operational conditions of the pilot plant and the degree of granulation determined the microbial community of the aerobic granules. Brachymonas, Zoogloea and Thauera were attributed with structural function as they are able to produce extracellular polymeric substances to maintain the granular structure. Nitrogen removal was justified by partial nitrification (Nitrosomonas) and denitrification (Thauera and Zoogloea), while Comamonas was identified as the main organic matter oxidizing bacteria. Overall, clear links between bacterial dynamics and composition with process performance were found and will help to predict their biological functions in wastewater ecosystems improving the future control of the process. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1212-1221, 2016. © 2016 American Institute of Chemical Engineers.

  18. Physicochemical characteristics and microbial community evolution of biofilms during the start-up period in a moving bed biofilm reactor.

    PubMed

    Zhu, Yan; Zhang, Yan; Ren, Hong-Qiang; Geng, Jin-Ju; Xu, Ke; Huang, Hui; Ding, Li-Li

    2015-03-01

    This study aimed to investigate biofilm properties evolution coupled with different ages during the start-up period in a moving bed biofilm reactor system. Physicochemical characteristics including adhesion force, extracellular polymeric substances (EPS), morphology as well as volatile solid and microbial community were studied. Results showed that the formation and development of biofilms exhibited four stages, including (I) initial attachment and young biofilm formation, (II) biofilms accumulation, (III) biofilm sloughing and updating, and (IV) biofilm maturation. During the whole start-up period, adhesion force was positively and significantly correlated with the contents of EPS, especially the content of polysaccharide. In addition, increased adhesion force and EPS were beneficial for biofilm retention. Gram-negative bacteria mainly including Sphaerotilus, Zoogloea and Haliscomenobacter were predominant in the initial stage. Actinobacteria was beneficial to resist sloughing. Furthermore, filamentous bacteria were dominant in maturation biofilm. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina high-throughput sequencing.

    PubMed

    Ma, Qiao; Qu, Yuanyuan; Shen, Wenli; Zhang, Zhaojing; Wang, Jingwei; Liu, Ziyan; Li, Duanxing; Li, Huijie; Zhou, Jiti

    2015-03-01

    In this study, Illumina high-throughput sequencing was used to reveal the community structures of nine coking wastewater treatment plants (CWWTPs) in China for the first time. The sludge systems exhibited a similar community composition at each taxonomic level. Compared to previous studies, some of the core genera in municipal wastewater treatment plants such as Zoogloea, Prosthecobacter and Gp6 were detected as minor species. Thiobacillus (20.83%), Comamonas (6.58%), Thauera (4.02%), Azoarcus (7.78%) and Rhodoplanes (1.42%) were the dominant genera shared by at least six CWWTPs. The percentages of autotrophic ammonia-oxidizing bacteria and nitrite-oxidizing bacteria were unexpectedly low, which were verified by both real-time PCR and fluorescence in situ hybridization analyses. Hierarchical clustering and canonical correspondence analysis indicated that operation mode, flow rate and temperature might be the key factors in community formation. This study provides new insights into our understanding of microbial community compositions and structures of CWWTPs. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. Nutrients removal and bacterial community structure for low C/N municipal wastewater using a modified anaerobic/anoxic/oxic (mA2/O) process in North China.

    PubMed

    Zhang, Shihua; Huang, Zhijia; Lu, Shujian; Zheng, Jun; Zhang, Xinxi

    2017-11-01

    A modified anaerobic/anoxic/oxic (mA2/O) process based on utilizing the internal carbon source and adding polypropylene carriers was operated for 90d to investigate the nutrients removal performance and bacterial community. This system exhibited a stable and efficient performance, particularly, in removing the NH 4 + -N and total phosphorus. The results of high-throughput sequencing showed that the 13 dominant genera containing Pseudomonas, Comamonas, Arcobacter, Nitrobacteria, Nitrosospira, Nitrosomonas, Bacteroides, Flavobacterium, Rhizobium, Acinetobacter, Zoogloea, Rhodocyclus and Moraxella were shared by five zones, inferring that they were the essential players in treating low C/N (below 5.0) municipal wastewater around 10°C. The average abundance of Nitrosospira (4.21%) was higher than that of Nitrosomonas (2.93%), suggested that Nitrosospira performed well under low temperature for nitrification. Additionally, both known Rhodocyclus-related PAOs and GAOs Competibacter were not detected possibly due to low temperature. Redundancy analysis (RDA) indicated that DO played more important roles in regulating bacterial community composition than HRT. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Effects of carbon sources and COD/N ratio on N2O emissions in subsurface flow constructed wetlands.

    PubMed

    Lyu, Wanlin; Huang, Lei; Xiao, Guangquan; Chen, Yucheng

    2017-12-01

    A set of constructed wetlands under two different carbon sources, namely, glucose (CW) and sodium acetate (YW), was established at a laboratory scale with influent COD/N ratios of 20:1, 10:1, 7:1, 4:1, and 0 to analyze the influence of carbon supply on nitrous oxide emissions. Results showed that the glucose systems generated higher N 2 O emissions than those of the sodium acetate systems. The higher amount of N 2 O-releasing fluxes in the CWs than in the YWs was consistent with the higher NO 2 - -N accumulation in the former than in the latter. Moreover, electron competition was tighter in the CWs and contributed to the incomplete denitrification with poor N 2 O production performance. Illumina MiSeq sequencing demonstrated that some denitrifying bacteria, such as Denitratisoma, Bacillus, and Zoogloea, were higher in the YWs than in the CWs. This result indicated that the carbon source is important in controlling N 2 O emissions in microbial communities. Copyright © 2017. Published by Elsevier Ltd.

  2. Response of microbial communities to pesticide residues in soil restored with Azolla imbricata.

    PubMed

    Lu, Xiao-Ming; Lu, Peng-Zhen

    2018-01-01

    Under conditions of Azolla imbricata restoration, the high-throughput sequencing technology was employed to determine change trends of microbial community structures in the soil that had undergone long-term application of pesticides. The relationship between the content of pesticide residues in the soil and the microbial community structure was analyzed. The results indicated that the microbial diversity was strongly negatively correlated with the contents of pesticide residues in the soil. At a suitable dosage of 5 kg fresh A. imbricata per square meter of soil area, the soil microbial diversity increased by 12.0%, and the contents of pesticide residues decreased by 26.8-72.1%. Sphingobacterium, Sphingopyxis, Thermincola, Sphingobium, Acaryochloris, Megasphaera, Ralstonia, Pseudobutyrivibrio, Desulfitobacterium, Nostoc, Oscillochloris, and Aciditerrimonas may play major roles in the degradation of pesticide residues. Thauera, Levilinea, Geothrix, Thiobacillus, Thioalkalispira, Desulfobulbus, Polycyclovorans, Fluviicola, Deferrisoma, Erysipelothrix, Desulfovibrio, Cytophaga, Vogesella, Zoogloea, Azovibrio, Halomonas, Paludibacter, Crocinitomix, Haliscomenobacter, Hirschia, Silanimonas, Alkalibacter, Woodsholea, Peredibacter, Leptolinea, Chitinivorax, Candidatus_Lumbricincola, Anaerovorax, Propionivibrio, Parasegetibacter, Byssovorax, Runella, Leptospira, and Nitrosomonas may be indicators to evaluate the contents of pesticide residues.

  3. Adaptation of microbial community of the anode biofilm in microbial fuel cells to temperature.

    PubMed

    Mei, Xiaoxue; Xing, Defeng; Yang, Yang; Liu, Qian; Zhou, Huihui; Guo, Changhong; Ren, Nanqi

    2017-10-01

    Temperature as an important ecological factor affects biofilm development and microbial metabolic activity. Here, the performances and microbial communities of microbial fuel cells (MFCs) at different temperature were analyzed. As the temperature decreased, the power output of MFCs declined. A maximum power density of 894.3±48.6mW/m 2 was obtained in MFCs operating at 30°C, which was 18.5% and 64.5% higher than that in MFCs at 20°C and 10°C, respectively. Illumina sequencing of 16S rRNA gene amplicons showed that a distinct difference in microbial community structure of the anode biofilms occurred. This resulted in different power outputs of MFCs. Species diversity analyses indicated that species evenness of the anode biofilms shifted beyond species richness at different temperatures. The predominant populations of the anode biofilm shifted from Geobacter and Azonexus (30°C) to Pelobacter (20°C) or Acidovorax, Zoogloea and Simplicispira, (10°C). These results indicate that temperature plays an important role in shaping microbial communities of the anode biofilms in MFCs through changes in species evenness. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Endogenous System Microbes as Treatment Process ...

    EPA Pesticide Factsheets

    Monitoring the efficacy of treatment strategies to remove pathogens in decentralized systems remains a challenge. Evaluating log reduction targets by measuring pathogen levels is hampered by their sporadic and low occurrence rates. Fecal indicator bacteria are used in centralized systems to indicate the presence of fecal pathogens, but are ineffective decentralized treatment process indicators as they generally occur at levels too low to assess log reduction targets. System challenge testing by spiking with high loads of fecal indicator organisms, like MS2 coliphage, has limitations, especially for large systems. Microbes that are endogenous to the decentralized system, occur in high abundances and mimic removal rates of bacterial, viral and/or parasitic protozoan pathogens during treatment could serve as alternative treatment process indicators to verify log reduction targets. To identify abundant microbes in wastewater, the bacterial and viral communities were examined using deep sequencing. Building infrastructure-associated bacteria, like Zoogloea, were observed as dominant members of the bacterial community in graywater. In blackwater, bacteriophage of the order Caudovirales constituted the majority of contiguous sequences from the viral community. This study identifies candidate treatment process indicators in decentralized systems that could be used to verify log removal during treatment. The association of the presence of treatment process indic

  5. Effect of short-time aerobic digestion on bioflocculation of extracellular polymeric substances from waste activated sludge.

    PubMed

    Zhang, Zhiqiang; Zhang, Jiao; Zhao, Jianfu; Xia, Siqing

    2015-02-01

    The effect of short-time aerobic digestion on bioflocculation of extracellular polymeric substances (EPSs) from waste activated sludge (WAS) was investigated. Bioflocculation of the EPS was found to be enhanced by 2∼6 h of WAS aerobic digestion under the conditions of natural sludge pH (about 7), high sludge concentration by gravity thickening, and dissolved oxygen of about 2 mg/L. With the same EPS extraction method, the total suspended solid content reduction of 0.20 and 0.36 g/L and the volatile suspended solid content reduction of 0.19 and 0.26 g/L were found for the WAS samples before and after aerobic digestion of 4 h. It indicates that more EPS is produced by short-time aerobic digestion of WAS. The scanning electron microscopy images of the WAS samples before and after aerobic digestion of 4 h showed that more EPS appeared on the surface of zoogloea by aerobic digestion, which reconfirmed that WAS aerobic digestion induced abundant formation of EPS. By WAS aerobic digestion, the flocculating rate of the EPS showed about 31 % growth, almost consistent with the growth of its yield (about 34 %). The EPSs obtained before and after the aerobic digestion presented nearly the same components, structures, and Fourier transform infrared spectra. These results revealed that short-time aerobic digestion of WAS enhanced the flocculation of the EPS by promoting its production.

  6. Proliferation of antibiotic resistance genes in microbial consortia of sequencing batch reactors (SBRs) upon exposure to trace erythromycin or erythromycin-H2O.

    PubMed

    Fan, Caian; He, Jianzhong

    2011-05-01

    A variety of antibiotics and their metabolites at sub-inhibitory level concentrations are suspected to expand resistance genes in the environment. However, knowledge is limited on the causal correlation of trace antibiotics or their metabolites with resistance proliferation. In this study, erythromycin (ERY) resistance genes were screened on microbial consortia of sequencing batch reactors (SBRs) after one year acclimation to ERY (100 μg/L) or dehydrated erythromycin (ERY-H(2)O, 50 μg/L). The identified esterase gene ereA explains that ERY could be degraded to six products by microbes acclimated to ERY (100 μg/L). However, ERY could not be degraded by microbes acclimated to ERY-H(2)O (50 μg/L), which may be due to the less proliferated ereA gene. Biodegradation of ERY required the presence of exogenous carbon source (e.g., glucose) and nutrients (e.g., nitrogen, phosphorus) for assimilation, but overdosed ammonium-N (>40 mg/L) inhibited degradation of ERY. Zoogloea, a kind of biofilm formation bacteria, became predominant in the ERY degradation consortia, suggesting that the input of ERY could induce biofilm resistance to antibiotics. Our study highlights that lower μg/L level of ERY or ERY-H(2)O in the environment encourages expansion of resistance genes in microbes. Copyright © 2011 Elsevier Ltd. All rights reserved.

  7. Characterization of the relative importance of human- and infrastructure-associated bacteria in grey water: a case study.

    PubMed

    Keely, S P; Brinkman, N E; Zimmerman, B D; Wendell, D; Ekeren, K M; De Long, S K; Sharvelle, S; Garland, J L

    2015-07-01

    Development of efficacious grey water (GW) treatment systems would benefit from detailed knowledge of the bacterial composition of GW. Thus, the aim of this study was to characterize the bacterial composition from (i) various points throughout a GW recycling system that collects shower and sink handwash (SH) water into an equalization tank (ET) prior to treatment and (ii) laundry (LA) water effluent of a commercial-scale washer. Bacterial composition was analysed by high-throughput pyrosequencing of the 16S rRNA gene. LA was dominated by skin-associated bacteria, with Corynebacterium, Staphylococcus, Micrococcus, Propionibacterium and Lactobacillus collectively accounting for nearly 50% of the total sequences. SH contained a more evenly distributed community than LA, with some overlap (e.g. Propionibacterium), but also contained distinct genera common to wastewater infrastructure (e.g. Zoogloea). The ET contained many of these same wastewater infrastructure-associated bacteria, but was dominated by genera adapted for anaerobic conditions. The data indicate that a relatively consistent set of skin-associated genera are the dominant human-associated bacteria in GW, but infrastructure-associated bacteria from the GW collection system and ET used for transient storage will be the most common bacteria entering GW treatment and reuse systems. This study is the first to use high-throughput sequencing to identify the bacterial composition of various GW sources. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

  8. Polyphasic analysis of an Azoarcus-Leptothrix-dominated bacterial biofilm developed on stainless steel surface in a gasoline-contaminated hypoxic groundwater.

    PubMed

    Benedek, Tibor; Táncsics, András; Szabó, István; Farkas, Milán; Szoboszlay, Sándor; Fábián, Krisztina; Maróti, Gergely; Kriszt, Balázs

    2016-05-01

    Pump and treat systems are widely used for hydrocarbon-contaminated groundwater remediation. Although biofouling (formation of clogging biofilms on pump surfaces) is a common problem in these systems, scarce information is available regarding the phylogenetic and functional complexity of such biofilms. Extensive information about the taxa and species as well as metabolic potential of a bacterial biofilm developed on the stainless steel surface of a pump submerged in a gasoline-contaminated hypoxic groundwater is presented. Results shed light on a complex network of interconnected hydrocarbon-degrading chemoorganotrophic and chemolitotrophic bacteria. It was found that besides the well-known hydrocarbon-degrading aerobic/facultative anaerobic biofilm-forming organisms (e.g., Azoarcus, Leptothrix, Acidovorax, Thauera, Pseudomonas, etc.), representatives of Fe(2+)-and Mn(2+)-oxidizing (Thiobacillus, Sideroxydans, Gallionella, Rhodopseudomonas, etc.) as well as of Fe(3+)- and Mn(4+)-respiring (Rhodoferax, Geobacter, Magnetospirillum, Sulfurimonas, etc.) bacteria were present in the biofilm. The predominance of β-Proteobacteria within the biofilm bacterial community in phylogenetic and functional point of view was revealed. Investigation of meta-cleavage dioxygenase and benzylsuccinate synthase (bssA) genes indicated that within the biofilm, Azoarcus, Leptothrix, Zoogloea, and Thauera species are most probably involved in intrinsic biodegradation of aromatic hydrocarbons. Polyphasic analysis of the biofilm shed light on the fact that subsurface microbial accretions might be reservoirs of novel putatively hydrocarbon-degrading bacterial species. Moreover, clogging biofilms besides their detrimental effects might supplement the efficiency of pump and treat systems.

  9. Bacterial communities in different locations, seasons and segments of a dairy wastewater treatment system consisting of six segments.

    PubMed

    Hirota, Kikue; Yokota, Yuji; Sekimura, Toru; Uchiumi, Hiroshi; Guo, Yong; Ohta, Hiroyuki; Yumoto, Isao

    2016-08-01

    A dairy wastewater treatment system composed of the 1st segment (no aeration) equipped with a facility for the destruction of milk fat particles, four successive aerobic treatment segments with activated sludge and a final sludge settlement segment was developed. The activated sludge is circulated through the six segments by settling sediments (activated sludge) in the 6th segment and sending the sediments beck to the 1st and 2nd segments. Microbiota was examined using samples from the non-aerated 1st and aerated 2nd segments obtained from two farms using the same system in summer or winter. Principal component analysis showed that the change in microbiota from the 1st to 2nd segments concomitant with effective wastewater treatment is affected by the concentrations of activated sludge and organic matter (biological oxygen demand [BOD]), and dissolved oxygen (DO) content. Microbiota from five segments (1st and four successive aerobic segments) in one location was also examined. Although the activated sludge is circulating throughout all the segments, microbiota fluctuation was observed. The observed successive changes in microbiota reflected the changes in the concentrations of organic matter and other physicochemical conditions (such as DO), suggesting that the microbiota is flexibly changeable depending on the environmental condition in the segments. The genera Dechloromonas, Zoogloea and Leptothrix are frequently observed in this wastewater treatment system throughout the analyses of microbiota in this study. Copyright © 2016. Published by Elsevier B.V.

  10. Use of in-field bioreactors demonstrate groundwater filtration influences planktonic bacterial community assembly, but not biofilm composition

    DOE PAGES

    Christensen, Geoffrey A.; Moon, Ji Won; Veach, Allison M.; ...

    2018-03-20

    Using in-field bioreactors, we investigated the influence of exogenous microorganisms in groundwater planktonic and biofilm microbial communities as part of the Integrated Field Research Challenge (IFRC). After an acclimation period with source groundwater, bioreactors received either filtered (0.22 μM filter) or unfiltered well groundwater in triplicate and communities were tracked routinely for 23 days after filtration was initiated. To address geochemical influences, the planktonic phase was assayed periodically for protein, organic acids, physico-/geochemical measurements and bacterial community (via 16S rRNA gene sequencing), while biofilms (i.e. microbial growth on sediment coupons) were targeted for bacterial community composition at the completion ofmore » the experiment (23 d). Based on Bray-Curtis distance, planktonic bacterial community composition varied temporally and between treatments (filtered, unfiltered bioreactors). Notably, filtration led to an increase in the dominant genus, Zoogloea relative abundance over time within the planktonic community, while remaining relatively constant when unfiltered. At day 23, biofilm communities were more taxonomically and phylogenetically diverse and substantially different from planktonic bacterial communities; however, the biofilm bacterial communities were similar regardless of filtration. These results suggest that although planktonic communities were sensitive to groundwater filtration, bacterial biofilm communities were stable and resistant to filtration. Bioreactors are useful tools in addressing questions pertaining to microbial community assembly and succession. These data provide a first step in understanding how an extrinsic factor, such as a groundwater inoculation and flux of microbial colonizers, impact how microbial communities assemble in environmental systems.« less

  11. Use of in-field bioreactors demonstrate groundwater filtration influences planktonic bacterial community assembly, but not biofilm composition

    PubMed Central

    Moon, JiWon; Veach, Allison M.; Mosher, Jennifer J.; Wymore, Ann M.; van Nostrand, Joy D.; Zhou, Jizhong; Hazen, Terry C.; Arkin, Adam P.; Elias, Dwayne A.

    2018-01-01

    Using in-field bioreactors, we investigated the influence of exogenous microorganisms in groundwater planktonic and biofilm microbial communities as part of the Integrated Field Research Challenge (IFRC). After an acclimation period with source groundwater, bioreactors received either filtered (0.22 μM filter) or unfiltered well groundwater in triplicate and communities were tracked routinely for 23 days after filtration was initiated. To address geochemical influences, the planktonic phase was assayed periodically for protein, organic acids, physico-/geochemical measurements and bacterial community (via 16S rRNA gene sequencing), while biofilms (i.e. microbial growth on sediment coupons) were targeted for bacterial community composition at the completion of the experiment (23 d). Based on Bray-Curtis distance, planktonic bacterial community composition varied temporally and between treatments (filtered, unfiltered bioreactors). Notably, filtration led to an increase in the dominant genus, Zoogloea relative abundance over time within the planktonic community, while remaining relatively constant when unfiltered. At day 23, biofilm communities were more taxonomically and phylogenetically diverse and substantially different from planktonic bacterial communities; however, the biofilm bacterial communities were similar regardless of filtration. These results suggest that although planktonic communities were sensitive to groundwater filtration, bacterial biofilm communities were stable and resistant to filtration. Bioreactors are useful tools in addressing questions pertaining to microbial community assembly and succession. These data provide a first step in understanding how an extrinsic factor, such as a groundwater inoculation and flux of microbial colonizers, impact how microbial communities assemble in environmental systems. PMID:29558522

  12. Use of in-field bioreactors demonstrate groundwater filtration influences planktonic bacterial community assembly, but not biofilm composition

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Christensen, Geoffrey A.; Moon, Ji Won; Veach, Allison M.

    Using in-field bioreactors, we investigated the influence of exogenous microorganisms in groundwater planktonic and biofilm microbial communities as part of the Integrated Field Research Challenge (IFRC). After an acclimation period with source groundwater, bioreactors received either filtered (0.22 μM filter) or unfiltered well groundwater in triplicate and communities were tracked routinely for 23 days after filtration was initiated. To address geochemical influences, the planktonic phase was assayed periodically for protein, organic acids, physico-/geochemical measurements and bacterial community (via 16S rRNA gene sequencing), while biofilms (i.e. microbial growth on sediment coupons) were targeted for bacterial community composition at the completion ofmore » the experiment (23 d). Based on Bray-Curtis distance, planktonic bacterial community composition varied temporally and between treatments (filtered, unfiltered bioreactors). Notably, filtration led to an increase in the dominant genus, Zoogloea relative abundance over time within the planktonic community, while remaining relatively constant when unfiltered. At day 23, biofilm communities were more taxonomically and phylogenetically diverse and substantially different from planktonic bacterial communities; however, the biofilm bacterial communities were similar regardless of filtration. These results suggest that although planktonic communities were sensitive to groundwater filtration, bacterial biofilm communities were stable and resistant to filtration. Bioreactors are useful tools in addressing questions pertaining to microbial community assembly and succession. These data provide a first step in understanding how an extrinsic factor, such as a groundwater inoculation and flux of microbial colonizers, impact how microbial communities assemble in environmental systems.« less

  13. Evaluation of the impact of dissolved oxygen concentration on biofilm microbial community in sequencing batch biofilm reactor.

    PubMed

    Wang, Jingyin; Rong, Hongwei; Zhang, Chaosheng

    2018-05-01

    The effect of dissolved oxygen concentration (DO) during simultaneous nitrification and denitrification (SND) was investigated in a sequencing batch biofilm reactor (SBBR). In addition, the removal rates of nitrogen and bacterial communities were investigated under different concentrations of DO (1.5, 3.5, and 4.5 mg/L). When the SND rate was 95.22%, the chemical oxygen demand and nitrogen removal was 92.22% and 84.15%, respectively, at 2.5 mg/L DO. The denitrification was inhibited by the increase of oxygen concentration. Microelectrode measurements showed that the thickness of oxygen penetration increased from 1.0 mm to 2.7 mm when the DO concentration increased from 1.5 mg/L to 5.5 mg/L. The current location of the aerobic and anaerobic layers in the biofilm was determined for analysis of the microbial community. High-throughput sequencing analysis revealed the communities of the biofilm approached similar structure and composition. Uliginosibacterium species, biofilm-forming bacteria Zoogloea species and Acinetobacter species were dominant. In the aerobic layer, phyla Betaproteobacteria and Saprospirae were predominant, the major phyla were shifted from Proteobacteria followed by Firmicutes and Bacteroidetes, which comprised 82% of the total sequences during the SND period. Anaerolineae was dominated in the anaerobic layer. The high abundance of Nitrospira in the aerobic biofilm provides evidence of the SND system performing better at ammonia oxidization. In addition, real-time PCR indicated that the amount of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) matched the Nitrospirales and Nitrosomonadales abundance well. Collectively, this study demonstrated the dynamics of key bacterial communities in the SND system were highly influenced by the DO concentration. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  14. Surface-attached and suspended bacterial community structure as affected by C/N ratios: relationship between bacteria and fish production.

    PubMed

    Yu, Ermeng; Xie, Jun; Wang, Jinlin; Ako, Harry; Wang, Guangjun; Chen, Zhanghe; Liu, Yongfeng

    2016-07-01

    Bacteria play crucial roles in the combined system of substrate addition and C/N control, which has been demonstrated to improve aquaculture production. However, the complexity of surface-attached bacteria on substrates and suspended bacteria in the water column hamper further application of this system. This study firstly applied this combined system into the culture of grass carp, and then explored the relationship between microbial complexes from surface-attached and suspended bacteria in this system and the production of grass carp. In addition, this study investigated bacterial community structures as affected by four C/N ratios using Illumina sequencing technology. The results demonstrated that the weight gain rate and specific growth rate of grass carp in the CN20 group (C/N ratio 20:1) were the highest (P < 0.05), and dietary supplementation of the microbial complex had positive effects on the growth of grass carp (P < 0.05). Sequencing data revealed that, (1) the proportions of Verrucomicrobiae and Rhodobacter (surface-attached), sediminibacterium (suspended), and emticicia (surface-attached and suspended) were much higher in the CN20 group compared with those in the other groups (P < 0.05); (2) Rhodobacter, Flavobacterium, Acinetobacter, Pseudomonas, Planctomyces, and Cloacibacterium might be important for the microbial colonization on substrates; (3) as the C/N ratio increased, proportions of Hydrogenophaga (surface-attached and suspended), Zoogloea, and Flectobacillus (suspended) increased, but proportions of Bacillus, Clavibacter, and Cellvibro (surface-attached and suspended) decreased. In summary, a combined system of substrate addition and C/N control increased the production of grass carp, and Verrucomicrobiae and Rhodobacter in the surface-attached bacterial community were potential probiotic bacteria that contributed to the enhanced growth of grass carp.

  15. Characterization of Co(III) EDTA-Reducing Bacteria in Metal- and Radionuclide-Contaminated Groundwater

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gao, Weimin; Gentry, Terry J; Mehlhorn, Tonia L

    The Waste Area Grouping 5 (WAG5) site at Oak Ridge National Laboratory has a potential to be a field site for evaluating the effectiveness of various bioremediation approaches and strategies. The site has been well studied in terms of its geological and geochemical properties over the past decade. However, despite the importance of microorganisms in bioremediation processes, the microbiological populations at the WAG5 site and their potential in bioremediation have not been similarly evaluated. In this study, we initiated research to characterize the microbial populations in WAG5 groundwater. Approximately 100 isolates from WAG5 groundwater were isolated and selected based onmore » colony morphology. Fifty-five unique isolates were identified by BOX-PCR and subjected to further characterization. 16S rRNA sequences indicated that these isolates belong to seventeen bacterial genera including Alcaligenes (1 isolate), Aquamonas (1), Aquaspirillum (1), Bacillus (10), Brevundimonas (5), Caulobacter (7), Dechloromonas (2), Janibacter (1), Janthinobacterium (2), Lactobacillus (1), Paenibacillus (4), Pseudomonas (9), Rhodoferax (1), Sphingomonas (1), Stenotrophomonas (6), Variovorax (2), and Zoogloea (1). Metal respiration assays identified several isolates, which phylogenically belong or are close to Caulobacter, Stenotrophomonas, Bacillus, Paenibacillus and Pseudomonas, capable of reducing Co(III)EDTA- to Co(II)EDTA{sup 2-} using the defined M1 medium under anaerobic conditions. In addition, using WAG5 groundwater directly as the inoculants, we found that organisms associated with WAG5 groundwater can reduce both Fe(III) and Co(III) under anaerobic conditions. Further assays were then performed to determine the optimal conditions for Co(III) reduction. These assays indicated that addition of various electron donors including ethanol, lactate, methanol, pyruvate, and acetate resulted in metal reduction. These experiments will provide useful background information for future bioremediation field experiments at the WAG5 site.« less

  16. Changes in the Structure and Function of Microbial Communities in Drinking Water Treatment Bioreactors upon Addition of Phosphorus▿ †

    PubMed Central

    Li, Xu; Upadhyaya, Giridhar; Yuen, Wangki; Brown, Jess; Morgenroth, Eberhard; Raskin, Lutgarde

    2010-01-01

    Phosphorus was added as a nutrient to bench-scale and pilot-scale biologically active carbon (BAC) reactors operated for perchlorate and nitrate removal from contaminated groundwater. The two bioreactors responded similarly to phosphorus addition in terms of microbial community function (i.e., reactor performance), while drastically different responses in microbial community structure were detected. Improvement in reactor performance with respect to perchlorate and nitrate removal started within a few days after phosphorus addition for both reactors. Microbial community structures were evaluated using molecular techniques targeting 16S rRNA genes. Clone library results showed that the relative abundance of perchlorate-reducing bacteria (PRB) Dechloromonas and Azospira in the bench-scale reactor increased from 15.2% and 0.6% to 54.2% and 11.7% after phosphorus addition, respectively. Real-time quantitative PCR (qPCR) experiments revealed that these increases started within a few days after phosphorus addition. In contrast, after phosphorus addition, the relative abundance of Dechloromonas in the pilot-scale reactor decreased from 7.1 to 0.6%, while Zoogloea increased from 17.9 to 52.0%. The results of this study demonstrated that similar operating conditions for bench-scale and pilot-scale reactors resulted in similar contaminant removal performances, despite dramatically different responses from microbial communities. These findings suggest that it is important to evaluate the microbial community compositions inside bioreactors used for drinking water treatment, as they determine the microbial composition in the effluent and impact downstream treatment requirements for drinking water production. This information could be particularly relevant to drinking water safety, if pathogens or disinfectant-resistant bacteria are detected in the bioreactors. PMID:20889793

  17. Unraveling the environmental and anthropogenic drivers of bacterial community changes in the Estuary of Bilbao and its tributaries.

    PubMed

    Aguirre, Mikel; Abad, David; Albaina, Aitor; Cralle, Lauren; Goñi-Urriza, María Soledad; Estonba, Andone; Zarraonaindia, Iratxe

    2017-01-01

    In this study, 16S rRNA gene sequencing was used to characterize the changes in taxonomic composition and environmental factors significantly influencing bacterial community structure across an annual cycle in the Estuary of Bilbao as well as its tributaries. In spite of this estuary being small and characterized by a short residence time, the environmental factors most highly correlated with the bacterial community mirrored those reported to govern larger estuaries, specifically salinity and temperature. Additionally, bacterial community changes in the estuary appeared to vary with precipitation. For example, an increase in freshwater bacteria (Comamonadaceae and Sphingobacteriaceae) was observed in high precipitation periods compared to the predominately marine-like bacteria (Rhodobacterales and Oceanospirillales) that were found in low precipitation periods. Notably, we observed a significantly higher relative abundance of Comamonadaceae than previously described in other estuaries. Furthermore, anthropic factors could have an impact on this particular estuary's bacterial community structure. For example, ecosystem changes related to the channelization of the estuary likely induced a low dissolved oxygen (DO) concentration, high temperature, and high chlorophyll concentration period in the inner euhaline water in summer (samples with salinity >30 ppt). Those samples were characterized by a high abundance of facultative anaerobes. For instance, OTUs classified as Cryomorphaceae and Candidatus Aquiluna rubra were negatively associated with DO concentration, while Oleiphilaceae was positively associated with DO concentration. Additionally, microorganisms related to biological treatment of wastewater (e.g Bdellovibrio and Zoogloea) were detected in the samples immediately downstream of the Bilbao Wastewater Treatment Plant (WWTP). There are several human activities planned in the region surrounding the Estuary of Bilbao (e.g. sediment draining, architectural changes, etc.) which will likely affect this ecosystem. Therefore, the addition of bacterial community profiling and diversity analysis into the estuary's ongoing monitoring program would provide a more comprehensive view of the ecological status of the Estuary of Bilbao.

  18. Unraveling the environmental and anthropogenic drivers of bacterial community changes in the Estuary of Bilbao and its tributaries

    PubMed Central

    Abad, David; Albaina, Aitor; Cralle, Lauren; Goñi-Urriza, María Soledad; Estonba, Andone; Zarraonaindia, Iratxe

    2017-01-01

    In this study, 16S rRNA gene sequencing was used to characterize the changes in taxonomic composition and environmental factors significantly influencing bacterial community structure across an annual cycle in the Estuary of Bilbao as well as its tributaries. In spite of this estuary being small and characterized by a short residence time, the environmental factors most highly correlated with the bacterial community mirrored those reported to govern larger estuaries, specifically salinity and temperature. Additionally, bacterial community changes in the estuary appeared to vary with precipitation. For example, an increase in freshwater bacteria (Comamonadaceae and Sphingobacteriaceae) was observed in high precipitation periods compared to the predominately marine-like bacteria (Rhodobacterales and Oceanospirillales) that were found in low precipitation periods. Notably, we observed a significantly higher relative abundance of Comamonadaceae than previously described in other estuaries. Furthermore, anthropic factors could have an impact on this particular estuary’s bacterial community structure. For example, ecosystem changes related to the channelization of the estuary likely induced a low dissolved oxygen (DO) concentration, high temperature, and high chlorophyll concentration period in the inner euhaline water in summer (samples with salinity >30 ppt). Those samples were characterized by a high abundance of facultative anaerobes. For instance, OTUs classified as Cryomorphaceae and Candidatus Aquiluna rubra were negatively associated with DO concentration, while Oleiphilaceae was positively associated with DO concentration. Additionally, microorganisms related to biological treatment of wastewater (e.g Bdellovibrio and Zoogloea) were detected in the samples immediately downstream of the Bilbao Wastewater Treatment Plant (WWTP). There are several human activities planned in the region surrounding the Estuary of Bilbao (e.g. sediment draining, architectural changes, etc.) which will likely affect this ecosystem. Therefore, the addition of bacterial community profiling and diversity analysis into the estuary’s ongoing monitoring program would provide a more comprehensive view of the ecological status of the Estuary of Bilbao. PMID:28594872

  19. The link of feast-phase dissolved oxygen (DO) with substrate competition and microbial selection in PHA production.

    PubMed

    Wang, Xiaofei; Oehmen, Adrian; Freitas, Elisabete B; Carvalho, Gilda; Reis, Maria A M

    2017-04-01

    Polyhydroxyalkanoates (PHAs) are biobased and biodegradable polyesters with the potential to replace conventional plastics. Aeration requires large amounts of energy in PHA production by mixed microbial cultures (MMCs), particularly during the feast phase due to substrate uptake. The objective of this study was to investigate the impact of DO concentrations on microbial selection, substrate competition and PHA production performance by MMCs. This represents the first study investigating DO impact on PHA production while feeding the multiple volatile fatty acids (VFAs) typically encountered in real fermented feedstocks, as well as the substrate preferences at different DO levels. Efficient microbial cultures were enriched under both high (3.47 ± 1.12 mg/L) and low (0.86 ± 0.50 mg/L) DO conditions in the feast phase containing mostly the same populations but with different relative abundance. The most abundant microorganisms in the two MMCs were Plasticicumulans, Zoogloea, Paracoccus, and Flavobacterium. Butyrate and valerate were found to be the preferred substrates as compared to acetate and propionate regardless of DO concentrations. In the accumulation step, the PHA storage capacity and yield were less affected by the change of DO levels when applying the culture selected under low DO in the feast phase (PHA storage capacity >60% and yield > 0.9 Cmol PHA/Cmol VFA). A high DO level is required for maximal PHA accumulation rates with the four VFAs (acetate, propionate, butyrate and valerate) present, due to the lower specific uptake rates of acetate and propionate under low DO conditions. However, butyrate and valerate specific uptake rates were less impacted by DO levels and hence low DO for PHA accumulation may be effective when feed is composed of these substrates only. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Microbial Population Dynamics and Ecosystem Functions of Anoxic/Aerobic Granular Sludge in Sequencing Batch Reactors Operated at Different Organic Loading Rates

    PubMed Central

    Szabó, Enikö; Liébana, Raquel; Hermansson, Malte; Modin, Oskar; Persson, Frank; Wilén, Britt-Marie

    2017-01-01

    The granular sludge process is an effective, low-footprint alternative to conventional activated sludge wastewater treatment. The architecture of the microbial granules allows the co-existence of different functional groups, e.g., nitrifying and denitrifying communities, which permits compact reactor design. However, little is known about the factors influencing community assembly in granular sludge, such as the effects of reactor operation strategies and influent wastewater composition. Here, we analyze the development of the microbiomes in parallel laboratory-scale anoxic/aerobic granular sludge reactors operated at low (0.9 kg m-3d-1), moderate (1.9 kg m-3d-1) and high (3.7 kg m-3d-1) organic loading rates (OLRs) and the same ammonium loading rate (0.2 kg NH4-N m-3d-1) for 84 days. Complete removal of organic carbon and ammonium was achieved in all three reactors after start-up, while the nitrogen removal (denitrification) efficiency increased with the OLR: 0% at low, 38% at moderate, and 66% at high loading rate. The bacterial communities at different loading rates diverged rapidly after start-up and showed less than 50% similarity after 6 days, and below 40% similarity after 84 days. The three reactor microbiomes were dominated by different genera (mainly Meganema, Thauera, Paracoccus, and Zoogloea), but these genera have similar ecosystem functions of EPS production, denitrification and polyhydroxyalkanoate (PHA) storage. Many less abundant but persistent taxa were also detected within these functional groups. The bacterial communities were functionally redundant irrespective of the loading rate applied. At steady-state reactor operation, the identity of the core community members was rather stable, but their relative abundances changed considerably over time. Furthermore, nitrifying bacteria were low in relative abundance and diversity in all reactors, despite their large contribution to nitrogen turnover. The results suggest that the OLR has considerable impact on the composition of the granular sludge communities, but also that the granule communities can be dynamic even at steady-state reactor operation due to high functional redundancy of several key guilds. Knowledge about microbial diversity with specific functional guilds under different operating conditions can be important for engineers to predict the stability of reactor functions during the start-up and continued reactor operation. PMID:28507540

  1. Impact of single walled carbon nanotubes (SWNTs) on wastewater microbial communities

    NASA Astrophysics Data System (ADS)

    Goyal, Deepankar

    Aim: Carbon nanotubes (CNTs) hold great promise in advancing our future, with potential applications such as adsorbents, conductive composites, energy storage devices, and more. Despite of numerous potential applications of CNTs, almost nothing so far is known about how such carbon-based nanomaterials would in future impact environmental processes such as wastewater treatment. The objective of the current study was to evaluate the impact of single-walled carbon nanotubes (SWNTs) on microbial communities and wastewater treatment processes in activated sludge bioreactors. Method: Closed system batch-scale reactors were used to simulate the activated sludge process. Two sets of triplicate reactors were analyzed to determine the effects of SWNTs and associated impurities compared to control reactors that contained no CNTs. Sub-samples for microbial community analyses were aseptically removed periodically from the bioreactors every ˜1 hour 15 minutes and held at -80°C until analyzed. Genomic DNA was extracted from bioreactor samples, and molecular profiles of the bacterial communities were determined using automated ribosomal intergenic spacer analysis (ARISA). The clones for the ARISA profiles having distinct ARISA peaks were picked and sequenced. Result: ARISA profiles revealed adverse changes in CNT-exposed bacterial communities compared to control reactors associated with CNTs. The phylogenetic analysis of cloned insert containing Internal Transcribed Spacer (ITS) region plus the 16S rRNA genes identified them belonging to taxonomic groups of the families Sphingomonadaceae and Cytophagacaceae , and the genus Zoogloea. Changes in community structure were observed in both SWNT-exposed and control reactors over the experimental time period. Also the date on which activated sludge was obtained from a wastewater treatment plant facility seemed to play a critical role in changing the community structure altogether, indicating the importance of analyzing microbial community structure in addition to abiotic and bulk biological factors when evaluating the potential impact of contaminants. In parallel to this study, another peer group made simultaneous measurements of chemical oxygen demand (COD) and abiotic factors, which indicated that while SWNTs adsorbed organic carbon (measured as COD), they did not significantly impact its degradation by the microbial community. Conclusion: These results indicate that SWNTs differentially impact members of the activated sludge reactor bacterial community. Significance of the Study: The finding that community structure was affected by SWNTs indicates that this emerging contaminant differentially impacted members of the activated sludge bacterial community, even on a short time period of exposure. These results raise the concern of SWNT impact on biological functions carried out by the activated sludge process and a question about their environmental impact in coming era of nanotechnology.

  2. Nitrogen Removal from Micro-Polluted Reservoir Water by Indigenous Aerobic Denitrifiers

    PubMed Central

    Huang, Ting-Lin; Zhou, Shi-Lei; Zhang, Hai-Han; Zhou, Na; Guo, Lin; Di, Shi-Yu; Zhou, Zi-Zhen

    2015-01-01

    Treatment of micro-polluted source water is receiving increasing attention because of environmental awareness on a global level. We isolated and identified aerobic denitrifying bacteria Zoogloea sp. N299, Acinetobacter sp. G107, and Acinetobacter sp. 81Y and used these to remediate samples of their native source water. We first domesticated the isolated strains in the source water, and the 48-h nitrate removal rates of strains N299, G107, and 81Y reached 33.69%, 28.28%, and 22.86%, respectively, with no nitrite accumulation. We then conducted a source-water remediation experiment and cultured the domesticated strains (each at a dry cell weight concentration of 0.4 ppm) together in a sample of source water at 20–26 °C and a dissolved oxygen concentration of 3–7 mg/L for 60 days. The nitrate concentration of the system decreased from 1.57 ± 0.02 to 0.42 ± 0.01 mg/L and that of a control system decreased from 1.63 ± 0.02 to 1.30 ± 0.01 mg/L, each with no nitrite accumulation. Total nitrogen of the bacterial system changed from 2.31 ± 0.12 to 1.09 ± 0.01 mg/L, while that of the control system changed from 2.51 ± 0.13 to 1.72 ± 0.06 mg/L. The densities of aerobic denitrification bacteria in the experimental and control systems ranged from 2.8 × 104 to 2 × 107 cfu/mL and from 7.75 × 103 to 5.5 × 105 cfu/mL, respectively. The permanganate index in the experimental and control systems decreased from 5.94 ± 0.12 to 3.10 ± 0.08 mg/L and from 6.02 ± 0.13 to 3.61 ± 0.11 mg/L, respectively, over the course of the experiment. Next, we supplemented samples of the experimental and control systems with additional bacteria or additional source water and cultivated the systems for another 35 days. The additional bacteria did little to improve the water quality. The additional source water provided supplemental carbon and brought the nitrate removal rate in the experimental system to 16.97%, while that in the control system reached only 3.01%, with no nitrite accumulation in either system. Our results show that aerobic denitrifying bacteria remain highly active after domestication and demonstrate the applicability of such organisms in the bioremediation of oligotrophic ecosystems. PMID:25867475

  3. The impact of microbial ecology and chemical profile on the enhanced biological phosphorus removal (EBPR) process: a case study of Northern Wastewater Treatment Works, Johannesburg.

    PubMed

    Kamika, Ilunga; Coetzee, Martie; Mamba, Bhekie Brilliance; Msagati, Titus; Momba, Maggy N B

    2014-03-10

    The impact of polyphosphate-accumulating organism (PAO) and glycogen-accumulating organism (GAO) populations as well as of the chemical profile on the performance of Unit-3 (open elutriation tanks) and Unit-5 (covered elutriation tank) of the City of Johannesburg Northern Wastewater Treatment Works was determined. Physicochemical parameters of wastewater samples were measured using standard methods. Bacterial diversity was determined using 16S rRNA gene amplicon pyrosequencing of the variable region V1-3. Results showed soluble COD concentrations from settled sewage for Unit-3 at 192.8 mg COD/L and for Unit-5 at 214.6 mg COD/L, which increased to 301.8 mg COD/L and 411.6 mg COD/L in the overflow from elutriation tanks and decreased to 170.9 mg COD/L and 256.3 mg COD/L at the division boxes, respectively. Both long-chain volatile fatty acids (heptanoic acid, isobutyric acid, 3-methylbutanoic acid, pentanoic acid, 4-methylpentanoic acid, methylheptanoic acid) and short-chain volatile fatty acids (acetic acid, propionic acid, isobutyric acid) were present within concentration ranges of 17.19 mg/L to 54.98 mg/L and 13.64 mg/L to 87.6 mg/L for Unit 3 and 38.61 mg/L to58.85 mg/L and 21.63 mg/L to 92.39 mg/L for Unit 5, respectively. In the secondary settling tanks, the phosphate-removal efficiency in Unit-5 appeared to be slightly higher (0.08 mg P/L) compared to that of Unit-3 (0.11 mg P/L). The average DO concentrations (2.1 mg/L and 2.2 mg/L) as well as the pH values (pH 7 to pH 7.5) were found to be slightly higher in Unit-5 in the aerobic zones. The high presence of PAOs in the bioreactors (Unit-5: Dechloromonas (14.96%), Acinetobacter (6.3%), Zoogloea (4.72%) in the anaerobic zone and Dechloromonas (22.37 %) in the aerobic zone; Unit-3: Dechloromonas (37.25%) in the anaerobic zone and Dechloromonas (23.97%) in the aerobic zone) confirmed the phosphate-removal efficiencies of both units. Negligible GAOs were found in the aerobic zones (Defluviicoccus spp.: 0.33% for Unit-5 and 0.68% for Unit-3) and in the anaerobic zones (Defluviicoccus: 9.8% for Unit-3). The high microbial diversity and a negligible percentage of GAOs in Unit-5 could contribute to its high phosphate-removal efficiency, although results did not indicate statistically significant differences between the unit with a covered elutriation tank (Unit-5) and that with open elutriation tanks (Unit-3).

  4. The Impact of Microbial Ecology and Chemical Profile on the Enhanced Biological Phosphorus Removal (EBPR) Process: A Case Study of Northern Wastewater Treatment Works, Johannesburg

    PubMed Central

    Kamika, Ilunga; Coetzee, Martie; Mamba, Bhekie Brilliance; Msagati, Titus; Momba, Maggy N. B.

    2014-01-01

    The impact of polyphosphate-accumulating organism (PAO) and glycogen-accumulating organism (GAO) populations as well as of the chemical profile on the performance of Unit-3 (open elutriation tanks) and Unit-5 (covered elutriation tank) of the City of Johannesburg Northern Wastewater Treatment Works was determined. Physicochemical parameters of wastewater samples were measured using standard methods. Bacterial diversity was determined using 16S rRNA gene amplicon pyrosequencing of the variable region V1-3. Results showed soluble COD concentrations from settled sewage for Unit-3 at 192.8 mg COD/L and for Unit-5 at 214.6 mg COD/L, which increased to 301.8 mg COD/L and 411.6 mg COD/L in the overflow from elutriation tanks and decreased to 170.9 mg COD/L and 256.3 mg COD/L at the division boxes, respectively. Both long-chain volatile fatty acids (heptanoic acid, isobutyric acid, 3-methylbutanoic acid, pentanoic acid, 4-methylpentanoic acid, methylheptanoic acid) and short-chain volatile fatty acids (acetic acid, propionic acid, isobutyric acid) were present within concentration ranges of 17.19 mg/L to 54.98 mg/L and 13.64 mg/L to 87.6 mg/L for Unit 3 and 38.61 mg/L to58.85 mg/L and 21.63 mg/L to 92.39 mg/L for Unit 5, respectively. In the secondary settling tanks, the phosphate-removal efficiency in Unit-5 appeared to be slightly higher (0.08 mg P/L) compared to that of Unit-3 (0.11 mg P/L). The average DO concentrations (2.1 mg/L and 2.2 mg/L) as well as the pH values (pH 7 to pH 7.5) were found to be slightly higher in Unit-5 in the aerobic zones. The high presence of PAOs in the bioreactors (Unit-5: Dechloromonas (14.96%), Acinetobacter (6.3%), Zoogloea (4.72%) in the anaerobic zone and Dechloromonas (22.37 %) in the aerobic zone; Unit-3: Dechloromonas (37.25%) in the anaerobic zone and Dechloromonas (23.97%) in the aerobic zone) confirmed the phosphate-removal efficiencies of both units. Negligible GAOs were found in the aerobic zones (Defluviicoccus spp.: 0.33% for Unit-5 and 0.68% for Unit-3) and in the anaerobic zones (Defluviicoccus: 9.8% for Unit-3). The high microbial diversity and a negligible percentage of GAOs in Unit-5 could contribute to its high phosphate-removal efficiency, although results did not indicate statistically significant differences between the unit with a covered elutriation tank (Unit-5) and that with open elutriation tanks (Unit-3). PMID:24619121

  5. The Role of Ethnic and National Identifications in Perceived Discrimination for Asian Americans: Toward a Better Understanding of the Buffering Effect of Group Identifications on Psychological Distress

    PubMed Central

    Huynh, Que-Lam; Devos, Thierry; Goldberg, Robyn

    2013-01-01

    A robust relationship between perceived racial discrimination and psychological distress has been established. Yet, mixed evidence exists regarding the extent to which ethnic identification moderates this relationship, and scarce attention has been paid to the moderating role of national identification. We propose that the role of group identifications in the perceived discrimination–psychological distress relationship is best understood by simultaneously and interactively considering ethnic and national identifications. A sample of 259 Asian American students completed measures of perceived discrimination, group identifications (specific ethnic identification stated by respondents and national or “mainstream American” identification), and psychological distress (anxiety and depression symptoms). Regression analyses revealed a significant three-way interaction of perceived discrimination, ethnic identification, and national identification on psychological distress. Simple-slope analyses indicated that dual identification (strong ethnic and national identifications) was linked to a weaker relationship between perceived discrimination and psychological distress compared with other group identification configurations. These findings underscore the need to consider the interconnections between ethnic and national identifications to better understand the circumstances under which group identifications are likely to buffer individuals against the adverse effects of racial discrimination. PMID:25258674

  6. Counter-Stereotypes and Feminism Promote Leadership Aspirations in Highly Identified Women.

    PubMed

    Leicht, Carola; Gocłowska, Małgorzata A; Van Breen, Jolien A; de Lemus, Soledad; Randsley de Moura, Georgina

    2017-01-01

    Although women who highly identify with other women are more susceptible to stereotype threat effects, women's identification might associate with greater leadership aspirations contingent on (1) counter-stereotype salience and (2) feminist identification. When gender counter-stereotypes are salient, women's identification should associate with greater leadership aspiration regardless of feminism, while when gender stereotypes are salient, women's identification would predict greater leadership aspirations contingent on a high level of feminist identification. In our study US-based women ( N = 208) attended to gender stereotypic (vs. counter-stereotypic) content. We measured identification with women and identification with feminism, and, following the manipulation, leadership aspirations in an imagined work scenario. The interaction between identification with women, identification with feminism, and attention to stereotypes (vs. counter-stereotypes) significantly predicted leadership aspirations. In the counter-stereotypic condition women's identification associated with greater leadership aspirations regardless of feminist identification. In the stereotypic condition women's identification predicted leadership aspirations only at high levels of feminist identification. We conclude that salient counter-stereotypes and a strong identification with feminism may help high women identifiers increase their leadership aspirations.

  7. Counter-Stereotypes and Feminism Promote Leadership Aspirations in Highly Identified Women

    PubMed Central

    Leicht, Carola; Gocłowska, Małgorzata A.; Van Breen, Jolien A.; de Lemus, Soledad; Randsley de Moura, Georgina

    2017-01-01

    Although women who highly identify with other women are more susceptible to stereotype threat effects, women's identification might associate with greater leadership aspirations contingent on (1) counter-stereotype salience and (2) feminist identification. When gender counter-stereotypes are salient, women's identification should associate with greater leadership aspiration regardless of feminism, while when gender stereotypes are salient, women's identification would predict greater leadership aspirations contingent on a high level of feminist identification. In our study US-based women (N = 208) attended to gender stereotypic (vs. counter-stereotypic) content. We measured identification with women and identification with feminism, and, following the manipulation, leadership aspirations in an imagined work scenario. The interaction between identification with women, identification with feminism, and attention to stereotypes (vs. counter-stereotypes) significantly predicted leadership aspirations. In the counter-stereotypic condition women's identification associated with greater leadership aspirations regardless of feminist identification. In the stereotypic condition women's identification predicted leadership aspirations only at high levels of feminist identification. We conclude that salient counter-stereotypes and a strong identification with feminism may help high women identifiers increase their leadership aspirations. PMID:28626437

  8. Establishing Viable and Effective Information-Warfare Capability in Developing Nations Based on the U.S. Model

    DTIC Science & Technology

    2012-12-01

    include law enforcement and intelligence capabilities in the lineup . However, national security strategy reflects the first four only. Figure 1...Term Joint Doctrine Identification Air Force Doctrine Identification Army Doctrine Identification Navy Doctrine Identification EW...59 Ibid., 39. 34 Term Joint Doctrine Identification Air Force Doctrine Identification Army Doctrine Identification Navy

  9. 49 CFR 1544.231 - Airport-approved and exclusive area personnel identification systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... carry out a personnel identification system for identification media that are airport-approved, or identification media that are issued for use in an exclusive area. The system must include the following: (1) Personnel identification media that— (i) Convey a full face image, full name, employer, and identification...

  10. Communications device identification methods, communications methods, wireless communications readers, wireless communications systems, and articles of manufacture

    DOEpatents

    Steele, Kerry D [Kennewick, WA; Anderson, Gordon A [Benton City, WA; Gilbert, Ronald W [Morgan Hill, CA

    2011-02-01

    Communications device identification methods, communications methods, wireless communications readers, wireless communications systems, and articles of manufacture are described. In one aspect, a communications device identification method includes providing identification information regarding a group of wireless identification devices within a wireless communications range of a reader, using the provided identification information, selecting one of a plurality of different search procedures for identifying unidentified ones of the wireless identification devices within the wireless communications range, and identifying at least some of the unidentified ones of the wireless identification devices using the selected one of the search procedures.

  11. New Methodology for Known Metabolite Identification in Metabonomics/Metabolomics: Topological Metabolite Identification Carbon Efficiency (tMICE).

    PubMed

    Sanchon-Lopez, Beatriz; Everett, Jeremy R

    2016-09-02

    A new, simple-to-implement and quantitative approach to assessing the confidence in NMR-based identification of known metabolites is introduced. The approach is based on a topological analysis of metabolite identification information available from NMR spectroscopy studies and is a development of the metabolite identification carbon efficiency (MICE) method. New topological metabolite identification indices are introduced, analyzed, and proposed for general use, including topological metabolite identification carbon efficiency (tMICE). Because known metabolite identification is one of the key bottlenecks in either NMR-spectroscopy- or mass spectrometry-based metabonomics/metabolomics studies, and given the fact that there is no current consensus on how to assess metabolite identification confidence, it is hoped that these new approaches and the topological indices will find utility.

  12. Utility of 16S rDNA Sequencing for Identification of Rare Pathogenic Bacteria.

    PubMed

    Loong, Shih Keng; Khor, Chee Sieng; Jafar, Faizatul Lela; AbuBakar, Sazaly

    2016-11-01

    Phenotypic identification systems are established methods for laboratory identification of bacteria causing human infections. Here, the utility of phenotypic identification systems was compared against 16S rDNA identification method on clinical isolates obtained during a 5-year study period, with special emphasis on isolates that gave unsatisfactory identification. One hundred and eighty-seven clinical bacteria isolates were tested with commercial phenotypic identification systems and 16S rDNA sequencing. Isolate identities determined using phenotypic identification systems and 16S rDNA sequencing were compared for similarity at genus and species level, with 16S rDNA sequencing as the reference method. Phenotypic identification systems identified ~46% (86/187) of the isolates with identity similar to that identified using 16S rDNA sequencing. Approximately 39% (73/187) and ~15% (28/187) of the isolates showed different genus identity and could not be identified using the phenotypic identification systems, respectively. Both methods succeeded in determining the species identities of 55 isolates; however, only ~69% (38/55) of the isolates matched at species level. 16S rDNA sequencing could not determine the species of ~20% (37/187) of the isolates. The 16S rDNA sequencing is a useful method over the phenotypic identification systems for the identification of rare and difficult to identify bacteria species. The 16S rDNA sequencing method, however, does have limitation for species-level identification of some bacteria highlighting the need for better bacterial pathogen identification tools. © 2016 Wiley Periodicals, Inc.

  13. Suspect/foil identification in actual crimes and in the laboratory: a reality monitoring analysis.

    PubMed

    Behrman, Bruce W; Richards, Regina E

    2005-06-01

    Four reality monitoring variables were used to discriminate suspect from foil identifications in 183 actual criminal cases. Four hundred sixty-one identification attempts based on five and six-person lineups were analyzed. These identification attempts resulted in 238 suspect identifications and 68 foil identifications. Confidence, automatic processing, eliminative processing and feature use comprised the set of reality monitoring variables. Thirty-five verbal confidence phrases taken from police reports were assigned numerical values on a 10-point confidence scale. Automatic processing identifications were those that occurred "immediately" or "without hesitation." Eliminative processing identifications occurred when witnesses compared or eliminated persons in the lineups. Confidence, automatic processing and eliminative processing were significant predictors, but feature use was not. Confidence was the most effective discriminator. In cases that involved substantial evidence extrinsic to the identification 43% of the suspect identifications were made with high confidence, whereas only 10% of the foil identifications were made with high confidence. The results of a laboratory study using the same predictors generally paralleled the archival results. Forensic implications are discussed.

  14. 14 CFR 45.13 - Identification data.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Identification data. 45.13 Section 45.13 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT IDENTIFICATION AND REGISTRATION MARKING Identification of Aircraft and Related Products § 45.13 Identification data...

  15. [A accurate identification method for Chinese materia medica--systematic identification of Chinese materia medica].

    PubMed

    Wang, Xue-Yong; Liao, Cai-Li; Liu, Si-Qi; Liu, Chun-Sheng; Shao, Ai-Juan; Huang, Lu-Qi

    2013-05-01

    This paper put forward a more accurate identification method for identification of Chinese materia medica (CMM), the systematic identification of Chinese materia medica (SICMM) , which might solve difficulties in CMM identification used the ordinary traditional ways. Concepts, mechanisms and methods of SICMM were systematically introduced and possibility was proved by experiments. The establishment of SICMM will solve problems in identification of Chinese materia medica not only in phenotypic characters like the mnorphous, microstructure, chemical constituents, but also further discovery evolution and classification of species, subspecies and population in medical plants. The establishment of SICMM will improve the development of identification of CMM and create a more extensive study space.

  16. Area-efficient physically unclonable function circuit architecture

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gurrieri, Thomas; Hamlet, Jason; Bauer, Todd

    Generating a physically a physically unclonable function ("PUF") circuit value includes comparing each of first identification components in a first bank to each of second identification components in a second bank. A given first identification component in the first bank is not compared to another first identification component in the first bank and a given second identification component in the second bank is not compared to another second identification component in the second bank. A digital bit value is generated for each comparison made while comparing each of the first identification components to each of the second identification components. Amore » PUF circuit value is generated from the digital bit values from each comparison made.« less

  17. Eyewitness Identification Reform: Data, Theory, and Due Process.

    PubMed

    Clark, Steven E

    2012-05-01

    Some commentators view my analyses (Clark, 2012, this issue) as an important step forward in assessing the costs and benefits of eyewitness identification reform. Others suggest that the trade-off between correct identifications lost and false identifications avoided is well-known; that the expected utility model is misspecified; and that the loss of correct identifications due to the use of reformed eyewitness identification procedures is irrelevant to policy decisions, as those correct identifications are the illegitimate product of suggestion and lucky guesses. Contrary to these criticisms, the loss of correct identifications has not been adequately considered in theoretical or policy matters, criticisms regarding the various utilities do not substantively change the nature of the trade-off, and the dismissal of lost correct identifications is based not on data but on an outdated theory of recognition memory. © The Author(s) 2012.

  18. Using Web-Based Key Character and Classification Instruction for Teaching Undergraduate Students Insect Identification

    NASA Astrophysics Data System (ADS)

    Golick, Douglas A.; Heng-Moss, Tiffany M.; Steckelberg, Allen L.; Brooks, David. W.; Higley, Leon G.; Fowler, David

    2013-08-01

    The purpose of the study was to determine whether undergraduate students receiving web-based instruction based on traditional, key character, or classification instruction differed in their performance of insect identification tasks. All groups showed a significant improvement in insect identifications on pre- and post-two-dimensional picture specimen quizzes. The study also determined student performance on insect identification tasks was not as good as for family-level identification as compared to broader insect orders and arthropod classification identification tasks. Finally, students erred significantly more by misidentification than misspelling specimen names on prepared specimen quizzes. Results of this study support that short web-based insect identification exercises can improve insect identification performance. Also included is a discussion of how these results can be used in teaching and future research on biological identification.

  19. 33 CFR 181.29 - Hull identification number display.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Hull identification number... SECURITY (CONTINUED) BOATING SAFETY MANUFACTURER REQUIREMENTS Identification of Boats § 181.29 Hull identification number display. Two identical hull identification numbers are required to be displayed on each...

  20. 30 CFR 45.3 - Identification of independent contractors.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Identification of independent contractors. 45.3... ADMINISTRATIVE REQUIREMENTS INDEPENDENT CONTRACTORS § 45.3 Identification of independent contractors. (a) Any independent contractor may obtain a permanent MSHA identification number. To obtain an identification number...

  1. Identification of genus Acinetobacter: Standardization of in-house PCR and its comparison with conventional phenotypic methods.

    PubMed

    Kulkarni, Sughosh S; Madalgi, Radhika; Ajantha, Ganavalli S; Kulkarni, Raghavendra D

    2017-01-01

    Acinetobacter is grouped under nonfermenting Gram-negative bacilli. It is increasingly isolated from pathological samples. The ability of this genus to acquire drug resistance and spread in the hospital settings is posing a grave problem in healthcare. Specific treatment protocols are advocated for Acinetobacter infections. Hence, rapid identification and drug susceptibility profiling are critical in the management of these infections. To standardize an in-house polymerase chain reaction (PCR) for identification of genus Acinetobacter and to compare PCR with two protocols for its phenotypic identification. A total of 96 clinical isolates of Acinetobacter were included in the study. An in-house PCR for genus level identification of Acinetobacter was standardized. All the isolates were phenotypically identified by two protocols. The results of PCR and phenotypic identification protocols were compared. The in-house PCR standardized was highly sensitive and specific for the genus Acinetobacter . There was 100% agreement between the phenotypic and molecular identification of the genus. The preliminary identification tests routinely used in clinical laboratories were also in complete agreement with phenotypic and molecular identification. The in-house PCR for genus level identification is specific and sensitive. However, it may not be essential for routine identification as the preliminary phenotypic identification tests used in the clinical laboratory reliably identify the genus Acinetobacter .

  2. 33 CFR 181.25 - Hull identification number format.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Hull identification number format... (CONTINUED) BOATING SAFETY MANUFACTURER REQUIREMENTS Identification of Boats § 181.25 Hull identification number format. Each of the hull identification numbers required by § 181.23 must consist of twelve...

  3. 30 CFR 77.215-1 - Refuse piles; identification.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Refuse piles; identification. 77.215-1 Section... COAL MINES Surface Installations § 77.215-1 Refuse piles; identification. A permanent identification marker, at least six feet high and showing the refuse pile identification number as assigned by the...

  4. An overview of the essential differences and similarities of system identification techniques

    NASA Technical Reports Server (NTRS)

    Mehra, Raman K.

    1991-01-01

    Information is given in the form of outlines, graphs, tables and charts. Topics include system identification, Bayesian statistical decision theory, Maximum Likelihood Estimation, identification methods, structural mode identification using a stochastic realization algorithm, and identification results regarding membrane simulations and X-29 flutter flight test data.

  5. The Identity and Identity Identification of Teachers

    ERIC Educational Resources Information Center

    Qu, Zhengwei

    2008-01-01

    When we tend to analyze the living conditions of teachers, system arrangement and identity identification can be considered a significant method for analysis. In reality, there appears a phenomenon of overlapping identification in the identity identification of teachers in China, which leads to plural selections in the identification manners of…

  6. 41 CFR 102-34.100 - Where is motor vehicle identification displayed?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.100 Where is motor vehicle identification displayed? Motor vehicle identification is displayed as follows: (a) For... Government motor vehicles may display motor vehicle identification on a decal in the rear window, or centered...

  7. 41 CFR 102-34.90 - What motor vehicle identification must we display on Government motor vehicles?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... identification must we display on Government motor vehicles? 102-34.90 Section 102-34.90 Public Contracts and... Vehicle Identification § 102-34.90 What motor vehicle identification must we display on Government motor...) Identification that readily identifies the agency owning the vehicle. ...

  8. 41 CFR 102-34.90 - What motor vehicle identification must we display on Government motor vehicles?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... identification must we display on Government motor vehicles? 102-34.90 Section 102-34.90 Public Contracts and... Vehicle Identification § 102-34.90 What motor vehicle identification must we display on Government motor...) Identification that readily identifies the agency owning the vehicle. ...

  9. 41 CFR 102-34.90 - What motor vehicle identification must we display on Government motor vehicles?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... identification must we display on Government motor vehicles? 102-34.90 Section 102-34.90 Public Contracts and... Vehicle Identification § 102-34.90 What motor vehicle identification must we display on Government motor...) Identification that readily identifies the agency owning the vehicle. ...

  10. 41 CFR 102-34.90 - What motor vehicle identification must we display on Government motor vehicles?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... identification must we display on Government motor vehicles? 102-34.90 Section 102-34.90 Public Contracts and... Vehicle Identification § 102-34.90 What motor vehicle identification must we display on Government motor...) Identification that readily identifies the agency owning the vehicle. ...

  11. 41 CFR 102-34.90 - What motor vehicle identification must we display on Government motor vehicles?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... identification must we display on Government motor vehicles? 102-34.90 Section 102-34.90 Public Contracts and... Vehicle Identification § 102-34.90 What motor vehicle identification must we display on Government motor...) Identification that readily identifies the agency owning the vehicle. ...

  12. 41 CFR 102-34.100 - Where is motor vehicle identification displayed?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.100 Where is motor vehicle identification displayed? Motor vehicle identification is displayed as follows: (a) For... Government motor vehicles may display motor vehicle identification on a decal in the rear window, or centered...

  13. 41 CFR 102-34.100 - Where is motor vehicle identification displayed?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.100 Where is motor vehicle identification displayed? Motor vehicle identification is displayed as follows: (a) For... Government motor vehicles may display motor vehicle identification on a decal in the rear window, or centered...

  14. 41 CFR 102-34.100 - Where is motor vehicle identification displayed?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.100 Where is motor vehicle identification displayed? Motor vehicle identification is displayed as follows: (a) For... Government motor vehicles may display motor vehicle identification on a decal in the rear window, or centered...

  15. Development of Teaching Materials for Field Identification of Plants and Analysis of Their Effectiveness in Science Education.

    ERIC Educational Resources Information Center

    Ohkawa, Chizuru

    2000-01-01

    Introduces teaching materials developed for field identification of plants with synoptical keys, identification tables, cards, and programs. Selects approximately 2000 seed plants and uses visibly identifiable characteristics for classification. Recommends using the methodology of identification in other areas for biological identification. (YDS)

  16. Phytophthora-ID.org: A sequence-based Phytophthora identification tool

    Treesearch

    N.J. Grünwald; F.N. Martin; M.M. Larsen; C.M. Sullivan; C.M. Press; M.D. Coffey; E.M. Hansen; J.L. Parke

    2010-01-01

    Contemporary species identification relies strongly on sequence-based identification, yet resources for identification of many fungal and oomycete pathogens are rare. We developed two web-based, searchable databases for rapid identification of Phytophthora spp. based on sequencing of the internal transcribed spacer (ITS) or the cytochrome oxidase...

  17. 33 CFR 181.29 - Hull identification number display.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Hull identification number... identification number display. Two identical hull identification numbers are required to be displayed on each boat hull. (a) The primary hull identification number must be affixed— (1) On boats with transoms, to...

  18. 33 CFR 181.29 - Hull identification number display.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Hull identification number... identification number display. Two identical hull identification numbers are required to be displayed on each boat hull. (a) The primary hull identification number must be affixed— (1) On boats with transoms, to...

  19. 16 CFR 303.20 - Registered identification numbers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Registered identification numbers. 303.20... identification numbers. (a) Registered numbers for use as the required identification in lieu of the name on... the form set out in paragraph (d) of this section. (b)(1) Registered identification numbers shall be...

  20. Proposing national identification number on dental prostheses as universal personal identification code - A revolution in forensic odontology

    PubMed Central

    Baad, Rajendra K.; Belgaumi, Uzma; Vibhute, Nupura; Kadashetti, Vidya; Chandrappa, Pramod Redder; Gugwad, Sushma

    2015-01-01

    The proper identification of a decedent is not only important for humanitarian and emotional reasons, but also for legal and administrative purposes. During the reconstructive identification process, all necessary information is gathered from the unknown body of the victim and hence that an objective reconstructed profile can be established. Denture marking systems are being used in various situations, and a number of direct and indirect methods are reported. We propose that national identification numbers be incorporated in all removable and fixed prostheses, so as to adopt a single and definitive universal personal identification code with the aim of achieving a uniform, standardized, easy, and fast identification method worldwide for forensic identification. PMID:26005294

  1. OPTICAL correlation identification technology applied in underwater laser imaging target identification

    NASA Astrophysics Data System (ADS)

    Yao, Guang-tao; Zhang, Xiao-hui; Ge, Wei-long

    2012-01-01

    The underwater laser imaging detection is an effective method of detecting short distance target underwater as an important complement of sonar detection. With the development of underwater laser imaging technology and underwater vehicle technology, the underwater automatic target identification has gotten more and more attention, and is a research difficulty in the area of underwater optical imaging information processing. Today, underwater automatic target identification based on optical imaging is usually realized with the method of digital circuit software programming. The algorithm realization and control of this method is very flexible. However, the optical imaging information is 2D image even 3D image, the amount of imaging processing information is abundant, so the electronic hardware with pure digital algorithm will need long identification time and is hard to meet the demands of real-time identification. If adopt computer parallel processing, the identification speed can be improved, but it will increase complexity, size and power consumption. This paper attempts to apply optical correlation identification technology to realize underwater automatic target identification. The optics correlation identification technology utilizes the Fourier transform characteristic of Fourier lens which can accomplish Fourier transform of image information in the level of nanosecond, and optical space interconnection calculation has the features of parallel, high speed, large capacity and high resolution, combines the flexibility of calculation and control of digital circuit method to realize optoelectronic hybrid identification mode. We reduce theoretical formulation of correlation identification and analyze the principle of optical correlation identification, and write MATLAB simulation program. We adopt single frame image obtained in underwater range gating laser imaging to identify, and through identifying and locating the different positions of target, we can improve the speed and orientation efficiency of target identification effectively, and validate the feasibility of this method primarily.

  2. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    PubMed

    Seng, Piseth; Drancourt, Michel; Gouriet, Frédérique; La Scola, Bernard; Fournier, Pierre-Edouard; Rolain, Jean Marc; Raoult, Didier

    2009-08-15

    Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry accurately identifies both selected bacteria and bacteria in select clinical situations. It has not been evaluated for routine use in the clinic. We prospectively analyzed routine MALDI-TOF mass spectrometry identification in parallel with conventional phenotypic identification of bacteria regardless of phylum or source of isolation. Discrepancies were resolved by 16S ribosomal RNA and rpoB gene sequence-based molecular identification. Colonies (4 spots per isolate directly deposited on the MALDI-TOF plate) were analyzed using an Autoflex II Bruker Daltonik mass spectrometer. Peptidic spectra were compared with the Bruker BioTyper database, version 2.0, and the identification score was noted. Delays and costs of identification were measured. Of 1660 bacterial isolates analyzed, 95.4% were correctly identified by MALDI-TOF mass spectrometry; 84.1% were identified at the species level, and 11.3% were identified at the genus level. In most cases, absence of identification (2.8% of isolates) and erroneous identification (1.7% of isolates) were due to improper database entries. Accurate MALDI-TOF mass spectrometry identification was significantly correlated with having 10 reference spectra in the database (P=.01). The mean time required for MALDI-TOF mass spectrometry identification of 1 isolate was 6 minutes for an estimated 22%-32% cost of current methods of identification. MALDI-TOF mass spectrometry is a cost-effective, accurate method for routine identification of bacterial isolates in <1 h using a database comprising > or =10 reference spectra per bacterial species and a 1.9 identification score (Brucker system). It may replace Gram staining and biochemical identification in the near future.

  3. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level.

    PubMed

    Cherkaoui, Abdessalam; Hibbs, Jonathan; Emonet, Stéphane; Tangomo, Manuela; Girard, Myriam; Francois, Patrice; Schrenzel, Jacques

    2010-04-01

    Bacterial identification relies primarily on culture-based methodologies requiring 24 h for isolation and an additional 24 to 48 h for species identification. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is an emerging technology newly applied to the problem of bacterial species identification. We evaluated two MALDI-TOF MS systems with 720 consecutively isolated bacterial colonies under routine clinical laboratory conditions. Isolates were analyzed in parallel on both devices, using the manufacturers' default recommendations. We compared MS with conventional biochemical test system identifications. Discordant results were resolved with "gold standard" 16S rRNA gene sequencing. The first MS system (Bruker) gave high-confidence identifications for 680 isolates, of which 674 (99.1%) were correct; the second MS system (Shimadzu) gave high-confidence identifications for 639 isolates, of which 635 (99.4%) were correct. Had MS been used for initial testing and biochemical identification used only in the absence of high-confidence MS identifications, the laboratory would have saved approximately US$5 per isolate in marginal costs and reduced average turnaround time by more than an 8-h shift, with no loss in accuracy. Our data suggest that implementation of MS as a first test strategy for one-step species identification would improve timeliness and reduce isolate identification costs in clinical bacteriology laboratories now.

  4. Dental x-ray image segmentation

    NASA Astrophysics Data System (ADS)

    Said, Eyad; Fahmy, Gamal F.; Nassar, Diaa; Ammar, Hany

    2004-08-01

    Law enforcement agencies have been exploiting biometric identifiers for decades as key tools in forensic identification. With the evolution in information technology and the huge volume of cases that need to be investigated by forensic specialists, it has become important to automate forensic identification systems. While, ante mortem (AM) identification, that is identification prior to death, is usually possible through comparison of many biometric identifiers, postmortem (PM) identification, that is identification after death, is impossible using behavioral biometrics (e.g. speech, gait). Moreover, under severe circumstances, such as those encountered in mass disasters (e.g. airplane crashers) or if identification is being attempted more than a couple of weeks postmortem, under such circumstances, most physiological biometrics may not be employed for identification, because of the decay of soft tissues of the body to unidentifiable states. Therefore, a postmortem biometric identifier has to resist the early decay that affects body tissues. Because of their survivability and diversity, the best candidates for postmortem biometric identification are the dental features. In this paper we present an over view about an automated dental identification system for Missing and Unidentified Persons. This dental identification system can be used by both law enforcement and security agencies in both forensic and biometric identification. We will also present techniques for dental segmentation of X-ray images. These techniques address the problem of identifying each individual tooth and how the contours of each tooth are extracted.

  5. [Measures to prevent patient identification errors in blood collection/physiological function testing utilizing a laboratory information system].

    PubMed

    Shimazu, Chisato; Hoshino, Satoshi; Furukawa, Taiji

    2013-08-01

    We constructed an integrated personal identification workflow chart using both bar code reading and an all in-one laboratory information system. The information system not only handles test data but also the information needed for patient guidance in the laboratory department. The reception terminals at the entrance, displays for patient guidance and patient identification tools at blood-sampling booths are all controlled by the information system. The number of patient identification errors was greatly reduced by the system. However, identification errors have not been abolished in the ultrasound department. After re-evaluation of the patient identification process in this department, we recognized that the major reason for the errors came from excessive identification workflow. Ordinarily, an ultrasound test requires patient identification 3 times, because 3 different systems are required during the entire test process, i.e. ultrasound modality system, laboratory information system and a system for producing reports. We are trying to connect the 3 different systems to develop a one-time identification workflow, but it is not a simple task and has not been completed yet. Utilization of the laboratory information system is effective, but is not yet perfect for patient identification. The most fundamental procedure for patient identification is to ask a person's name even today. Everyday checks in the ordinary workflow and everyone's participation in safety-management activity are important for the prevention of patient identification errors.

  6. 22 CFR 41.32 - Nonresident alien Mexican border crossing identification cards; combined border crossing...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. 41.32 Section 41.32 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF NONIMMIGRANTS UNDER THE... crossing identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. (a...

  7. 22 CFR 41.32 - Nonresident alien Mexican border crossing identification cards; combined border crossing...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. 41.32 Section 41.32 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF NONIMMIGRANTS UNDER THE... crossing identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. (a...

  8. 22 CFR 41.32 - Nonresident alien Mexican border crossing identification cards; combined border crossing...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. 41.32 Section 41.32 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF NONIMMIGRANTS UNDER THE... crossing identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. (a...

  9. 22 CFR 41.32 - Nonresident alien Mexican border crossing identification cards; combined border crossing...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. 41.32 Section 41.32 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF NONIMMIGRANTS UNDER THE... crossing identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. (a...

  10. 22 CFR 41.32 - Nonresident alien Mexican border crossing identification cards; combined border crossing...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. 41.32 Section 41.32 Foreign Relations DEPARTMENT OF STATE VISAS VISAS: DOCUMENTATION OF NONIMMIGRANTS UNDER THE... crossing identification cards; combined border crossing identification cards and B-1/B-2 visitor visas. (a...

  11. Inertial parameter identification using contact force information for an unknown object captured by a space manipulator

    NASA Astrophysics Data System (ADS)

    Chu, Zhongyi; Ma, Ye; Hou, Yueyang; Wang, Fengwen

    2017-02-01

    This paper presents a novel identification method for the intact inertial parameters of an unknown object in space captured by a manipulator in a space robotic system. With strong dynamic and kinematic coupling existing in the robotic system, the inertial parameter identification of the unknown object is essential for the ideal control strategy based on changes in the attitude and trajectory of the space robot via capturing operations. Conventional studies merely refer to the principle and theory of identification, and an error analysis process of identification is deficient for a practical scenario. To solve this issue, an analysis of the effect of errors on identification is illustrated first, and the accumulation of measurement or estimation errors causing poor identification precision is demonstrated. Meanwhile, a modified identification equation incorporating the contact force, as well as the force/torque of the end-effector, is proposed to weaken the accumulation of errors and improve the identification accuracy. Furthermore, considering a severe disturbance condition caused by various measured noises, the hybrid immune algorithm, Recursive Least Squares and Affine Projection Sign Algorithm (RLS-APSA), is employed to decode the modified identification equation to ensure a stable identification property. Finally, to verify the validity of the proposed identification method, the co-simulation of ADAMS-MATLAB is implemented by multi-degree of freedom models of a space robotic system, and the numerical results show a precise and stable identification performance, which is able to guarantee the execution of aerospace operations and prevent failed control strategies.

  12. Religious and national group identification in adolescence: a study among three religious groups in Mauritius.

    PubMed

    Ng Tseung-Wong, Caroline; Verkuyten, Maykel

    2013-01-01

    Religious group identification is an important but understudied social identity. The present study investigates religious group identification among adolescents of different faiths (Hindu, Muslim, Christian) living in multicultural Mauritius. It further explores how religious and national group identities come together among religious majority and minority adolescents. For three age groups (11 to 19 years, N = 2152) we examined the strength of adolescents' religious and national group identification, the associations between these two identities, and the relationships to global self-esteem. Across age and religious group, participants reported stronger identification with their religious group than with the nation. Identification with both categories declined with age, with the exception of Muslims, whose strong religious identification was found across adolescence. The association between religious and national identification was positive, albeit stronger for the majority group of Hindus and for early adolescents. We examined the manner in which religious and national identities come together using a direct self-identification measure and by combining the separate continuous measures of identification. Four distinct clusters of identification (predominant religious identifiers, dual identifiers, neutrals, and separate individuals) that were differently associated with global self-esteem were found. Dual identifiers reported the highest level of global self-esteem. The clusters of identification did not fully correspond to the findings for the direct self-identification measure. The results are discussed in terms of the meaning of dual identity and the positive manner in which adolescents can manage their multiple identities while taking into account the ideological framework in which those identities are played out.

  13. Comparison of phenotypic methods and matrix-assisted laser desorption ionisation time-of-flight mass spectrometry for the identification of aero-tolerant Actinomyces spp. isolated from soft-tissue infections.

    PubMed

    Ng, L S Y; Sim, J H C; Eng, L C; Menon, S; Tan, T Y

    2012-08-01

    Aero-tolerant Actinomyces spp. are an under-recognised cause of cutaneous infections, in part because identification using conventional phenotypic methods is difficult and may be inaccurate. Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) is a promising new technique for bacterial identification, but with limited data on the identification of aero-tolerant Actinomyces spp. This study evaluated the accuracy of a phenotypic biochemical kit, MALDI-TOF MS and genotypic identification methods for the identification of this problematic group of organisms. Thirty aero-tolerant Actinomyces spp. were isolated from soft-tissue infections over a 2-year period. Species identification was performed by 16 s rRNA sequencing and genotypic results were compared with results obtained by API Coryne and MALDI-TOF MS. There was poor agreement between API Coryne and genotypic identification, with only 33% of isolates correctly identified to the species level. MALDI-TOF MS correctly identified 97% of isolates to the species level, with 33% of identifications achieved with high confidence scores. MALDI-TOF MS is a promising new tool for the identification of aero-tolerant Actinomyces spp., but improvement of the database is required in order to increase the confidence level of identification.

  14. Effectiveness of Reptile Species Identification--A Comparison of a Dichotomous Key with an Identification Book

    ERIC Educational Resources Information Center

    Randler, Christoph; Zehender, Irene

    2006-01-01

    Species identification tasks are a prerequisite for an understanding of biodiversity. Here, we focused on different educational materials to foster the identification of six European reptile species. Our educational training unit was based on natural plastic models of six species and pupils either used an illustrated identification book or a…

  15. 21 CFR 830.320 - Submission of unique device identification information.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Identification Database § 830.320 Submission of unique device identification information. (a) Designation of... Unique Device Identification Database (GUDID) in a format that we can process, review, and archive...

  16. Examining relations of entertainment with social interaction motives and team identification.

    PubMed

    Gau, Li-Shiue; Wann, Daniel L; James, Jeffrey D

    2010-10-01

    The goal was to investigate a potential causal pattern between the motives of sport spectators and team identification by using a cross-lagged panel design. Questionnaires were completed by 229 participants at the beginning and end of one NCAA (National Collegiate Athletic Association) college football season for 4 mo. in the USA. The questionnaire included three items for each motive (Social Interaction and Entertainment), three items measuring team identification, and demographic items. The relation of Entertainment at Time 1 to team identification at Time 2 was larger than the relation of team identification at Time 1 to Entertainment at Time 2. This suggests that the motivation of Entertainment may lead to the formation of team identification more than team identification leads to Entertainment. However, the motive of Social Interaction did not show the same pattern. As results of this study suggested some spectator sport motives might lead to identification with a team, the hypothesis that all motives would be the basis of team identification might not be correct.

  17. [The effect of suggestibility on eyewitness identifications: A comparison between showups and lineups].

    PubMed

    Miura, Hiroshi; Itoh, Yuji

    2016-04-01

    There are two types of eyewitness-identification procedures: showups and lineups. Although the false-identification rate of showups was considered to be higher than that of lineups, experimental research has not always supported the superiority of lineups. Further, suggestibility of showups is believed to produce higher false-identification rates, but no experimental study has manipulated suggestibility. In this study, we manipulated suggestibility; 258 participants performed photo identification in a showup or lineup. The results revealed that the correct-identification rate was higher in the showups than the lineups, and the rate of dangerous false identification for the innocent suspect did not differ between showups and lineups. In lineups alone, the false-identification rate of the high-suggestibility.condition was marginally higher than that of the low-suggestibility condition. The results indicate that suggestibility, which results from the preconception that the perpetrator must exist in the photos, increases false identifications in relative judgments, such as in lineups.

  18. 29 CFR 1611.4 - Times, places, and requirements for identification of individuals making requests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... present sufficient personal identification (e.g., driver's license, employee identification card, social security card, credit cards). If the requester is unable to provide such identification, the requester...

  19. 21 CFR 830.340 - Voluntary submission of ancillary device identification information.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Identification Database § 830.340 Voluntary submission of ancillary device identification information. (a) You may not submit any information to the Global Unique Device Identification Database (GUDID) other than...

  20. A New Paradigm for Known Metabolite Identification in Metabonomics/Metabolomics: Metabolite Identification Efficiency

    PubMed Central

    Everett, Jeremy R.

    2015-01-01

    A new paradigm is proposed for assessing confidence in the identification of known metabolites in metabonomics studies using NMR spectroscopy approaches. This new paradigm is based upon the analysis of the amount of metabolite identification information retrieved from NMR spectra relative to the molecular size of the metabolite. Several new indices are proposed including: metabolite identification efficiency (MIE) and metabolite identification carbon efficiency (MICE), both of which can be easily calculated. These indices, together with some guidelines, can be used to provide a better indication of known metabolite identification confidence in metabonomics studies than existing methods. Since known metabolite identification in untargeted metabonomics studies is one of the key bottlenecks facing the science currently, it is hoped that these concepts based on molecular spectroscopic informatics, will find utility in the field. PMID:25750701

  1. A new paradigm for known metabolite identification in metabonomics/metabolomics: metabolite identification efficiency.

    PubMed

    Everett, Jeremy R

    2015-01-01

    A new paradigm is proposed for assessing confidence in the identification of known metabolites in metabonomics studies using NMR spectroscopy approaches. This new paradigm is based upon the analysis of the amount of metabolite identification information retrieved from NMR spectra relative to the molecular size of the metabolite. Several new indices are proposed including: metabolite identification efficiency (MIE) and metabolite identification carbon efficiency (MICE), both of which can be easily calculated. These indices, together with some guidelines, can be used to provide a better indication of known metabolite identification confidence in metabonomics studies than existing methods. Since known metabolite identification in untargeted metabonomics studies is one of the key bottlenecks facing the science currently, it is hoped that these concepts based on molecular spectroscopic informatics, will find utility in the field.

  2. [Advances of studies on new technology and method for identifying traditional Chinese medicinal materials].

    PubMed

    Chen, Shilin; Guo, Baolin; Zhang, Guijun; Yan, Zhuyun; Luo, Guangming; Sun, Suqin; Wu, Hezhen; Huang, Linfang; Pang, Xiaohui; Chen, Jianbo

    2012-04-01

    In this review, the authors summarized the new technologies and methods for identifying traditional Chinese medicinal materials, including molecular identification, chemical identification, morphological identification, microscopic identification and identification based on biological effects. The authors introduced the principle, characteristics, application and prospect on each new technology or method and compared their advantages and disadvantages. In general, new methods make the result more objective and accurate. DNA barcoding technique and spectroscopy identification have their owner obvious strongpoint in universality and digitalization. In the near future, the two techniques are promising to be the main trend for identifying traditional Chinese medicinal materials. The identification techniques based on microscopy, liquid chromatography, PCR, biological effects and DNA chip will be indispensable supplements. However, the bionic identification technology is just placed in the developing stage at present.

  3. Evaluation of the utility of a glycemic pattern identification system.

    PubMed

    Otto, Erik A; Tannan, Vinay

    2014-07-01

    With the increasing prevalence of systems allowing automated, real-time transmission of blood glucose data there is a need for pattern recognition techniques that can inform of deleterious patterns in glycemic control when people test. We evaluated the utility of pattern identification with a novel pattern identification system named Vigilant™ and compared it to standard pattern identification methods in diabetes. To characterize the importance of an identified pattern we evaluated the relative risk of future hypoglycemic and hyperglycemic events in diurnal periods following identification of a pattern in a data set of 536 patients with diabetes. We evaluated events 2 days, 7 days, 30 days, and 61-90 days from pattern identification, across diabetes types and cohorts of glycemic control, and also compared the system to 6 pattern identification methods consisting of deleterious event counts and percentages over 5-, 14-, and 30-day windows. Episodes of hypoglycemia, hyperglycemia, severe hypoglycemia, and severe hyperglycemia were 120%, 46%, 123%, and 76% more likely after pattern identification, respectively, compared to periods when no pattern was identified. The system was also significantly more predictive of deleterious events than other pattern identification methods evaluated, and was persistently predictive up to 3 months after pattern identification. The system identified patterns that are significantly predictive of deleterious glycemic events, and more so relative to many pattern identification methods used in diabetes management today. Further study will inform how improved pattern identification can lead to improved glycemic control. © 2014 Diabetes Technology Society.

  4. 41 CFR 102-34.105 - Before we sell a motor vehicle, what motor vehicle identification must we remove?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... vehicle, what motor vehicle identification must we remove? 102-34.105 Section 102-34.105 Public Contracts... Vehicle Identification § 102-34.105 Before we sell a motor vehicle, what motor vehicle identification must we remove? You must remove all motor vehicle identification before you transfer the title or deliver...

  5. 41 CFR 102-34.105 - Before we sell a motor vehicle, what motor vehicle identification must we remove?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... vehicle, what motor vehicle identification must we remove? 102-34.105 Section 102-34.105 Public Contracts... Vehicle Identification § 102-34.105 Before we sell a motor vehicle, what motor vehicle identification must we remove? You must remove all motor vehicle identification before you transfer the title or deliver...

  6. 41 CFR 102-34.105 - Before we sell a motor vehicle, what motor vehicle identification must we remove?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... vehicle, what motor vehicle identification must we remove? 102-34.105 Section 102-34.105 Public Contracts... Vehicle Identification § 102-34.105 Before we sell a motor vehicle, what motor vehicle identification must we remove? You must remove all motor vehicle identification before you transfer the title or deliver...

  7. 41 CFR 102-34.105 - Before we sell a motor vehicle, what motor vehicle identification must we remove?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... vehicle, what motor vehicle identification must we remove? 102-34.105 Section 102-34.105 Public Contracts... Vehicle Identification § 102-34.105 Before we sell a motor vehicle, what motor vehicle identification must we remove? You must remove all motor vehicle identification before you transfer the title or deliver...

  8. 41 CFR 102-34.105 - Before we sell a motor vehicle, what motor vehicle identification must we remove?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... vehicle, what motor vehicle identification must we remove? 102-34.105 Section 102-34.105 Public Contracts... Vehicle Identification § 102-34.105 Before we sell a motor vehicle, what motor vehicle identification must we remove? You must remove all motor vehicle identification before you transfer the title or deliver...

  9. Performance Assessment of the CapitalBio Mycobacterium Identification Array System for Identification of Mycobacteria

    PubMed Central

    Liu, Jingbo; Yan, Zihe; Han, Min; Han, Zhijun; Jin, Lingjie; Zhao, Yanlin

    2012-01-01

    The CapitalBio Mycobacterium identification microarray system is a rapid system for the detection of Mycobacterium tuberculosis. The performance of this system was assessed with 24 reference strains, 486 Mycobacterium tuberculosis clinical isolates, and 40 clinical samples and then compared to the “gold standard” of DNA sequencing. The CapitalBio Mycobacterium identification microarray system showed highly concordant identification results of 100% and 98.4% for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM), respectively. The sensitivity and specificity of the CapitalBio Mycobacterium identification array for identification of Mycobacterium tuberculosis isolates were 99.6% and 100%, respectively, for direct detection and identification of clinical samples, and the overall sensitivity was 52.5%. It was 100% for sputum, 16.7% for pleural fluid, and 10% for bronchoalveolar lavage fluid, respectively. The total assay was completed in 6 h, including DNA extraction, PCR, and hybridization. The results of this study confirm the utility of this system for the rapid identification of mycobacteria and suggest that the CapitalBio Mycobacterium identification array is a molecular diagnostic technique with high sensitivity and specificity that has the capacity to quickly identify most mycobacteria. PMID:22090408

  10. When teachers go the extra mile: foci of organisational identification as determinants of different forms of organisational citizenship behaviour among schoolteachers.

    PubMed

    Christ, Oliver; van Dick, Rolf; Wagner, Ulrich; Stellmacher, Jost

    2003-09-01

    Psychological variables should play an important role in determining teachers' involvement in behaviours not directly or formally forced by contracts. Organisational identification as proposed from the Social Identity Approach is examined as a possible determinant of organisational citizenship behaviour (OCB) among schoolteachers. The aim of the present study was to explore the relationships between different foci of organisational identification and different forms of OCB in schools. Data sets of altogether 447 German school teachers who filled in all relevant items in a cross-sectional questionnaire are used for analyses in the present study. Standardised questionnaires measuring organisational identification and OCB were administered. Exploratory and confirmatory factor analyses revealed the proposed foci of identification (i.e., career identification, team identification, and organisational identification), as well as different forms of OCB (i.e., OCB towards the own qualification, towards the team, and towards the organisation). Structural equation modelling supports the main hypothesis that foci of identification relate differentially to forms of OCB. The results emphasise the importance of organisational identification as a determinant of OCB in schools. Practical implications are discussed.

  11. Multi-centre evaluation of mass spectrometric identification of anaerobic bacteria using the VITEK® MS system.

    PubMed

    Garner, O; Mochon, A; Branda, J; Burnham, C-A; Bythrow, M; Ferraro, M; Ginocchio, C; Jennemann, R; Manji, R; Procop, G W; Richter, S; Rychert, J; Sercia, L; Westblade, L; Lewinski, M

    2014-04-01

    Accurate and timely identification of anaerobic bacteria is critical to successful treatment. Classic phenotypic methods for identification require long turnaround times and can exhibit poor species level identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an identification method that can provide rapid identification of anaerobes. We present a multi-centre study assessing the clinical performance of the VITEK(®) MS in the identification of anaerobic bacteria. Five different test sites analysed a collection of 651 unique anaerobic isolates comprising 11 different genera. Multiple species were included for several of the genera. Briefly, anaerobic isolates were applied directly to a well of a target plate. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry. Mass spectra results were generated with the VITEK(®) MS, and the comparative spectral analysis and organism identification were determined using the VITEK(®) MS database 2.0. Results were confirmed by 16S rRNA gene sequencing. Of the 651 isolates analysed, 91.2% (594/651) exhibited the correct species identification. An additional eight isolates were correctly identified to genus level, raising the rate of identification to 92.5%. Genus-level identification consisted of Actinomyces, Bacteroides and Prevotella species. Fusobacterium nucleatum, Actinomyces neuii and Bacteroides uniformis were notable for an increased percentage of no-identification results compared with the other anaerobes tested. VITEK(®) MS identification of clinically relevant anaerobes is highly accurate and represents a dramatic improvement over other phenotypic methods in accuracy and turnaround time. © 2013 The Authors Clinical Microbiology and Infection © 2013 European Society of Clinical Microbiology and Infectious Diseases.

  12. 78 FR 68817 - Proposed Information Collection; Comment Request; Northeast Region Gear Identification

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-15

    ... provide vessel and gear identification information (e.g., hull identification number, Federal fishing... submitted to the NMFS as a result of this collection. The vessel's hull identification number or other means...

  13. TOXICITY IDENTIFICATION EVALUATION (TIE) RESULTS FOR METAL CONTAMINATED SEDIMENTS

    EPA Science Inventory

    Identification of contaminants in sediment is necessary for sound management decisions on sediment disposal, remediation, determination of ecological risk, and source identification. We have been developing sediment toxicity identification evaluation (TIE) techniques that allow ...

  14. [Applications of DNA identification technology in protection of wild animals].

    PubMed

    Ni, Ping-Ya; Pei, Li; Ge, Wen-Dong; Zhang, Ying; Yang, Xue-Ying; Xu, Xiao-Yu; Tu, Zheng

    2011-12-01

    With the development of biotechnology, forensic DNA identification technology in protection of wild animals has been used more and more widely. This review introduces the global status of wildlife crime and the relevant protection to wildlife, outlines the practical applications of forensic DNA identification technology with regard to species identification, determination of geographic origin, individual identification and paternity identification. It focus on the techniques commonly used in DNA typing and their merits and demerits, as well as the problems and prospects of forensic DNA technology for wildlife conservation.

  15. Dysregulation in level of goal and action identification across psychological disorders.

    PubMed

    Watkins, Edward

    2011-03-01

    Goals, events, and actions can be mentally represented within a hierarchical framework that ranges from more abstract to more concrete levels of identification. A more abstract level of identification involves general, superordinate, and decontextualized mental representations that convey the meaning of goals, events, and actions, "why" an action is performed, and its purpose, ends, and consequences. A more concrete level of identification involves specific and subordinate mental representations that include contextual details of goals, events, and actions, and the specific "how" details of an action. This review considers three lines of evidence for considering that dysregulation of level of goal/action identification may be a transdiagnostic process. First, there is evidence that different levels of identification have distinct functional consequences and that in non-clinical samples level of goal/action identification appears to be regulated in a flexible and adaptive way to match the level of goal/action identification to circumstances. Second, there is evidence that level of goal/action identification causally influences symptoms and processes involved in psychological disorders, including emotional response, repetitive thought, impulsivity, problem solving and procrastination. Third, there is evidence that the level of goal/action identification is biased and/or dysregulated in certain psychological disorders, with a bias towards more abstract identification for negative events in depression, GAD, PTSD, and social anxiety. Copyright © 2010 Elsevier Ltd. All rights reserved.

  16. Comparison of Five System Identification Algorithms for Rotorcraft Higher Harmonic Control

    NASA Technical Reports Server (NTRS)

    Jacklin, Stephen A.

    1998-01-01

    This report presents an analysis and performance comparison of five system identification algorithms. The methods are presented in the context of identifying a frequency-domain transfer matrix for the higher harmonic control (HHC) of helicopter vibration. The five system identification algorithms include three previously proposed methods: (1) the weighted-least- squares-error approach (in moving-block format), (2) the Kalman filter method, and (3) the least-mean-squares (LMS) filter method. In addition there are two new ones: (4) a generalized Kalman filter method and (5) a generalized LMS filter method. The generalized Kalman filter method and the generalized LMS filter method were derived as extensions of the classic methods to permit identification by using more than one measurement per identification cycle. Simulation results are presented for conditions ranging from the ideal case of a stationary transfer matrix and no measurement noise to the more complex cases involving both measurement noise and transfer-matrix variation. Both open-loop identification and closed- loop identification were simulated. Closed-loop mode identification was more challenging than open-loop identification because of the decreasing signal-to-noise ratio as the vibration became reduced. The closed-loop simulation considered both local-model identification, with measured vibration feedback and global-model identification with feedback of the identified uncontrolled vibration. The algorithms were evaluated in terms of their accuracy, stability, convergence properties, computation speeds, and relative ease of implementation.

  17. Dysregulation in level of goal and action identification across psychological disorders

    PubMed Central

    Watkins, Edward

    2011-01-01

    Goals, events, and actions can be mentally represented within a hierarchical framework that ranges from more abstract to more concrete levels of identification. A more abstract level of identification involves general, superordinate, and decontextualized mental representations that convey the meaning of goals, events, and actions, “why” an action is performed, and its purpose, ends, and consequences. A more concrete level of identification involves specific and subordinate mental representations that include contextual details of goals, events, and actions, and the specific “how” details of an action. This review considers three lines of evidence for considering that dysregulation of level of goal/action identification may be a transdiagnostic process. First, there is evidence that different levels of identification have distinct functional consequences and that in non-clinical samples level of goal/action identification appears to be regulated in a flexible and adaptive way to match the level of goal/action identification to circumstances. Second, there is evidence that level of goal/action identification causally influences symptoms and processes involved in psychological disorders, including emotional response, repetitive thought, impulsivity, problem solving and procrastination. Third, there is evidence that the level of goal/action identification is biased and/or dysregulated in certain psychological disorders, with a bias towards more abstract identification for negative events in depression, GAD, PTSD, and social anxiety. PMID:20579789

  18. A survey on the utility of the USEPA CADDIS stressor identification procedure.

    PubMed

    Harwood, John J; Stroud, Robert Adam

    2012-06-01

    The Environmental Protection Agency (EPA) has made available on the worldwide web a systematic stream stressor identification procedure, the "Causal Analysis/Diagnosis Decision Information System" or CADDIS. We report here the results of a survey of regulators and scientists in 11 states who use CADDIS or another stressor identification procedure in their work. The 13 survey questions address guidelines as to what impairment scenarios to approach with stressor identification, what information is needed to perform stressor identification, and what the stakeholder role is in performing stressor identification. At the time of this survey (the summer of 2009), the EPA CADDIS website was less commonly used among the state regulators surveyed than the published EPA stressor identification document on which it is based. The respondents generally find the EPA stressor identification procedure useful and capable of being adapted to their individual needs. Survey respondents all use stressor identification in their Total Maximum Daily Load work, but also in a wide variety of other applications. All the "types of evidence" included in the CADDIS stressor identification procedure are used by the practitioners surveyed with the exception of the results of ecological simulation models. While the CADDIS documentation encourages the involvement of stakeholders in stressor identification, most respondents do not assemble stakeholder teams of local officials and citizens to participate in stressor analyses.

  19. Post-traumatic growth enhances social identification in liver transplant patients: A longitudinal study.

    PubMed

    Scrignaro, Marta; Sani, Fabio; Wakefield, Juliet Ruth Helen; Bianchi, Elisabetta; Magrin, Maria Elena; Gangeri, Laura

    2016-09-01

    The main aim of this paper is to investigate the prediction that greater subjective identification with relevant groups and social categories (i.e. 'family' and 'transplantees') can be an outcome of post-traumatic growth (PTG). To date there are no studies that have explored these relationships. A longitudinal study was conducted with a group of 100 liver transplant patients from the outpatient populations of the participating centre. Data were collected by means of a self-report questionnaire, which was completed at two different time points (T1 and T2) that were 24months apart. PTG was assessed using the Post-Traumatic Growth Inventory, while both transplantee and family identification were assessed using group identification scales. A path model was tested, using a structural equation model (SEM) approach, to examine the reciprocal effects among family identification, transplantee identification, and PTG over time. As predicted, we found that greater PTG T1 predicted both greater family identification T2 and marginally greater transplantee identification T2. However, the two identification variables did not predict PTG over time. The results show that family identification and transplantee identification may be outcomes of the PTG process, confirming the importance of adopting a thriving multidimensional model of adjustment to medical illness, whereby people facing adverse life events, such as transplantation, may flourish rather than deteriorate psychologically. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Phenomenological reports diagnose accuracy of eyewitness identification decisions.

    PubMed

    Palmer, Matthew A; Brewer, Neil; McKinnon, Anna C; Weber, Nathan

    2010-02-01

    This study investigated whether measuring the phenomenology of eyewitness identification decisions aids evaluation of their accuracy. Witnesses (N=502) viewed a simulated crime and attempted to identify two targets from lineups. A divided attention manipulation during encoding reduced the rate of remember (R) correct identifications, but not the rates of R foil identifications or know (K) judgments in the absence of recollection (i.e., K/[1-R]). Both RK judgments and recollection ratings (a novel measure of graded recollection) distinguished correct from incorrect positive identifications. However, only recollection ratings improved accuracy evaluation after identification confidence was taken into account. These results provide evidence that RK judgments for identification decisions function in a similar way as for recognition decisions; are consistent with the notion of graded recollection; and indicate that measures of phenomenology can enhance the evaluation of identification accuracy. Copyright 2009 Elsevier B.V. All rights reserved.

  1. Identification and stochastic control of helicopter dynamic modes

    NASA Technical Reports Server (NTRS)

    Molusis, J. A.; Bar-Shalom, Y.

    1983-01-01

    A general treatment of parameter identification and stochastic control for use on helicopter dynamic systems is presented. Rotor dynamic models, including specific applications to rotor blade flapping and the helicopter ground resonance problem are emphasized. Dynamic systems which are governed by periodic coefficients as well as constant coefficient models are addressed. The dynamic systems are modeled by linear state variable equations which are used in the identification and stochastic control formulation. The pure identification problem as well as the stochastic control problem which includes combined identification and control for dynamic systems is addressed. The stochastic control problem includes the effect of parameter uncertainty on the solution and the concept of learning and how this is affected by the control's duel effect. The identification formulation requires algorithms suitable for on line use and thus recursive identification algorithms are considered. The applications presented use the recursive extended kalman filter for parameter identification which has excellent convergence for systems without process noise.

  2. Word-identification priming for ignored and attended words

    NASA Technical Reports Server (NTRS)

    Stone, M.; Ladd, S. L.; Vaidya, C. J.; Gabrieli, J. D.

    1998-01-01

    Three experiments examined contributions of study phase awareness of word identity to subsequent word-identification priming by manipulating visual attention to words at study. In Experiment 1, word-identification priming was reduced for ignored relative to attended words, even though ignored words were identified sufficiently to produce negative priming in the study phase. Word-identification priming was also reduced after color naming relative to emotional valence rating (Experiment 2) or word reading (Experiment 3), even though an effect of emotional valence upon color naming (Experiment 2) indicated that words were identified at study. Thus, word-identification priming was reduced even when word identification occurred at study. Word-identification priming may depend on awareness of word identity at the time of study.

  3. 49 CFR 574.5 - Tire identification requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Tire identification requirements. 574.5 Section... SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) TIRE IDENTIFICATION AND RECORDKEEPING § 574.5 Tire identification requirements. Each tire manufacturer shall conspicuously label on one...

  4. Unique identification code for medical fundus images using blood vessel pattern for tele-ophthalmology applications.

    PubMed

    Singh, Anushikha; Dutta, Malay Kishore; Sharma, Dilip Kumar

    2016-10-01

    Identification of fundus images during transmission and storage in database for tele-ophthalmology applications is an important issue in modern era. The proposed work presents a novel accurate method for generation of unique identification code for identification of fundus images for tele-ophthalmology applications and storage in databases. Unlike existing methods of steganography and watermarking, this method does not tamper the medical image as nothing is embedded in this approach and there is no loss of medical information. Strategic combination of unique blood vessel pattern and patient ID is considered for generation of unique identification code for the digital fundus images. Segmented blood vessel pattern near the optic disc is strategically combined with patient ID for generation of a unique identification code for the image. The proposed method of medical image identification is tested on the publically available DRIVE and MESSIDOR database of fundus image and results are encouraging. Experimental results indicate the uniqueness of identification code and lossless recovery of patient identity from unique identification code for integrity verification of fundus images. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  5. Patterns of Cognitive Strengths and Weaknesses: Identification Rates, Agreement, and Validity for Learning Disabilities Identification

    PubMed Central

    Miciak, Jeremy; Fletcher, Jack M.; Stuebing, Karla; Vaughn, Sharon; Tolar, Tammy D.

    2014-01-01

    Purpose Few empirical investigations have evaluated LD identification methods based on a pattern of cognitive strengths and weaknesses (PSW). This study investigated the reliability and validity of two proposed PSW methods: the concordance/discordance method (C/DM) and cross battery assessment (XBA) method. Methods Cognitive assessment data for 139 adolescents demonstrating inadequate response to intervention was utilized to empirically classify participants as meeting or not meeting PSW LD identification criteria using the two approaches, permitting an analysis of: (1) LD identification rates; (2) agreement between methods; and (3) external validity. Results LD identification rates varied between the two methods depending upon the cut point for low achievement, with low agreement for LD identification decisions. Comparisons of groups that met and did not meet LD identification criteria on external academic variables were largely null, raising questions of external validity. Conclusions This study found low agreement and little evidence of validity for LD identification decisions based on PSW methods. An alternative may be to use multiple measures of academic achievement to guide intervention. PMID:24274155

  6. Predicting nurses' acceptance of radiofrequency identification technology.

    PubMed

    Norten, Adam

    2012-10-01

    The technology of radiofrequency identification allows for the scanning of radiofrequency identification-tagged objects and individuals without line-of-sight requirements. Healthcare organizations use radiofrequency identification to ensure the health and safety of patients and medical personnel and to uncover inefficiencies. Although the successful implementation of a system incorporating radiofrequency identification technologies requires acceptance and use of the technology, some nurses using radiofrequency identification in hospitals feel like "Big Brother" is watching them. This predictive study used a theoretical model assessing the effect of five independent variables: privacy concerns, attitudes, subjective norms, controllability, and self-efficacy, on a dependent variable, nurses' behavioral intention to use radiofrequency identification. A Web-based questionnaire containing previously validated questions was answered by 106 US RNs. Multiple linear regression showed that all constructs together accounted for 60% of the variance in nurses' intention to use radiofrequency identification. Of the predictors in the model, attitudes provided the largest unique contribution when the other predictors in the model were held constant; subjective norms also provided a unique contribution. Privacy concerns, controllability, and self-efficacy did not provide a significant contribution to nurses' behavioral intention to use radiofrequency identification.

  7. Algorithm improvement program nuclide identification algorithm scoring criteria and scoring application.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Enghauser, Michael

    2016-02-01

    The goal of the Domestic Nuclear Detection Office (DNDO) Algorithm Improvement Program (AIP) is to facilitate gamma-radiation detector nuclide identification algorithm development, improvement, and validation. Accordingly, scoring criteria have been developed to objectively assess the performance of nuclide identification algorithms. In addition, a Microsoft Excel spreadsheet application for automated nuclide identification scoring has been developed. This report provides an overview of the equations, nuclide weighting factors, nuclide equivalencies, and configuration weighting factors used by the application for scoring nuclide identification algorithm performance. Furthermore, this report presents a general overview of the nuclide identification algorithm scoring application including illustrative examples.

  8. Identification of propulsion systems

    NASA Technical Reports Server (NTRS)

    Merrill, Walter; Guo, Ten-Huei; Duyar, Ahmet

    1991-01-01

    This paper presents a tutorial on the use of model identification techniques for the identification of propulsion system models. These models are important for control design, simulation, parameter estimation, and fault detection. Propulsion system identification is defined in the context of the classical description of identification as a four step process that is unique because of special considerations of data and error sources. Propulsion system models are described along with the dependence of system operation on the environment. Propulsion system simulation approaches are discussed as well as approaches to propulsion system identification with examples for both air breathing and rocket systems.

  9. Damage Identification of Piles Based on Vibration Characteristics

    PubMed Central

    Zhang, Xiaozhong; Yao, Wenjuan; Chen, Bo; Liu, Dewen

    2014-01-01

    A method of damage identification of piles was established by using vibration characteristics. The approach focused on the application of the element strain energy and sensitive modals. A damage identification equation of piles was deduced using the structural vibration equation. The equation contained three major factors: change rate of element modal strain energy, damage factor of pile, and sensitivity factor of modal damage. The sensitive modals of damage identification were selected by using sensitivity factor of modal damage firstly. Subsequently, the indexes for early-warning of pile damage were established by applying the change rate of strain energy. Then the technology of computational analysis of wavelet transform was used to damage identification for pile. The identification of small damage of pile was completely achieved, including the location of damage and the extent of damage. In the process of identifying the extent of damage of pile, the equation of damage identification was used in many times. Finally, a stadium project was used as an example to demonstrate the effectiveness of the proposed method of damage identification for piles. The correctness and practicability of the proposed method were verified by comparing the results of damage identification with that of low strain test. The research provided a new way for damage identification of piles. PMID:25506062

  10. Identification of bacteria isolated from veterinary clinical specimens using MALDI-TOF MS.

    PubMed

    Pavlovic, Melanie; Wudy, Corinna; Zeller-Peronnet, Veronique; Maggipinto, Marzena; Zimmermann, Pia; Straubinger, Alix; Iwobi, Azuka; Märtlbauer, Erwin; Busch, Ulrich; Huber, Ingrid

    2015-01-01

    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has recently emerged as a rapid and accurate identification method for bacterial species. Although it has been successfully applied for the identification of human pathogens, it has so far not been well evaluated for routine identification of veterinary bacterial isolates. This study was performed to compare and evaluate the performance of MALDI-TOF MS based identification of veterinary bacterial isolates with commercially available conventional test systems. Discrepancies of both methods were resolved by sequencing 16S rDNA and, if necessary, the infB gene for Actinobacillus isolates. A total of 375 consecutively isolated veterinary samples were collected. Among the 357 isolates (95.2%) correctly identified at the genus level by MALDI-TOF MS, 338 of them (90.1% of the total isolates) were also correctly identified at the species level. Conventional methods offered correct species identification for 319 isolates (85.1%). MALDI-TOF identification therefore offered more accurate identification of veterinary bacterial isolates. An update of the in-house mass spectra database with additional reference spectra clearly improved the identification results. In conclusion, the presented data suggest that MALDI-TOF MS is an appropriate platform for classification and identification of veterinary bacterial isolates.

  11. Development of a multilocus-based approach for sponge (phylum Porifera) identification: refinement and limitations.

    PubMed

    Yang, Qi; Franco, Christopher M M; Sorokin, Shirley J; Zhang, Wei

    2017-02-02

    For sponges (phylum Porifera), there is no reliable molecular protocol available for species identification. To address this gap, we developed a multilocus-based Sponge Identification Protocol (SIP) validated by a sample of 37 sponge species belonging to 10 orders from South Australia. The universal barcode COI mtDNA, 28S rRNA gene (D3-D5), and the nuclear ITS1-5.8S-ITS2 region were evaluated for their suitability and capacity for sponge identification. The highest Bit Score was applied to infer the identity. The reliability of SIP was validated by phylogenetic analysis. The 28S rRNA gene and COI mtDNA performed better than the ITS region in classifying sponges at various taxonomic levels. A major limitation is that the databases are not well populated and possess low diversity, making it difficult to conduct the molecular identification protocol. The identification is also impacted by the accuracy of the morphological classification of the sponges whose sequences have been submitted to the database. Re-examination of the morphological identification further demonstrated and improved the reliability of sponge identification by SIP. Integrated with morphological identification, the multilocus-based SIP offers an improved protocol for more reliable and effective sponge identification, by coupling the accuracy of different DNA markers.

  12. Development of a multilocus-based approach for sponge (phylum Porifera) identification: refinement and limitations

    PubMed Central

    Yang, Qi; Franco, Christopher M. M.; Sorokin, Shirley J.; Zhang, Wei

    2017-01-01

    For sponges (phylum Porifera), there is no reliable molecular protocol available for species identification. To address this gap, we developed a multilocus-based Sponge Identification Protocol (SIP) validated by a sample of 37 sponge species belonging to 10 orders from South Australia. The universal barcode COI mtDNA, 28S rRNA gene (D3–D5), and the nuclear ITS1-5.8S-ITS2 region were evaluated for their suitability and capacity for sponge identification. The highest Bit Score was applied to infer the identity. The reliability of SIP was validated by phylogenetic analysis. The 28S rRNA gene and COI mtDNA performed better than the ITS region in classifying sponges at various taxonomic levels. A major limitation is that the databases are not well populated and possess low diversity, making it difficult to conduct the molecular identification protocol. The identification is also impacted by the accuracy of the morphological classification of the sponges whose sequences have been submitted to the database. Re-examination of the morphological identification further demonstrated and improved the reliability of sponge identification by SIP. Integrated with morphological identification, the multilocus-based SIP offers an improved protocol for more reliable and effective sponge identification, by coupling the accuracy of different DNA markers. PMID:28150727

  13. Evoking and Measuring Identification with Narrative Characters – A Linguistic Cues Framework

    PubMed Central

    van Krieken, Kobie; Hoeken, Hans; Sanders, José

    2017-01-01

    Current research on identification with narrative characters poses two problems. First, although identification is seen as a dynamic process of which the intensity varies during reading, it is usually measured by means of post-reading questionnaires containing self-report items. Second, it is not clear which linguistic characteristics evoke identification. The present paper proposes that an interdisciplinary framework allows for more precise manipulations and measurements of identification, which will ultimately advance our understanding of the antecedents and nature of this process. The central hypothesis of our Linguistic Cues Framework is that identification with a narrative character is a multidimensional experience for which different dimensions are evoked by different linguistic cues. The first part of the paper presents a literature review on identification, resulting in a renewed conceptualization of identification which distinguishes six dimensions: a spatiotemporal, a perceptual, a cognitive, a moral, an emotional, and an embodied dimension. The second part argues that each of these dimensions is influenced by specific linguistic cues which represent various aspects of the narrative character’s perspective. The proposed relations between linguistic cues and identification dimensions are specified in six propositions. The third part discusses what psychological and neurocognitive methods enable the measurement of the various identification dimensions in order to test the propositions. By establishing explicit connections between the linguistic characteristics of narratives and readers’ physical, psychological, and neurocognitive responses to narratives, this paper develops a research agenda for future empirical research on identification with narrative characters. PMID:28751875

  14. Improving substructure identification accuracy of shear structures using virtual control system

    NASA Astrophysics Data System (ADS)

    Zhang, Dongyu; Yang, Yang; Wang, Tingqiang; Li, Hui

    2018-02-01

    Substructure identification is a powerful tool to identify the parameters of a complex structure. Previously, the authors developed an inductive substructure identification method for shear structures. The identification error analysis showed that the identification accuracy of this method is significantly influenced by the magnitudes of two key structural responses near a certain frequency; if these responses are unfavorable, the method cannot provide accurate estimation results. In this paper, a novel method is proposed to improve the substructure identification accuracy by introducing a virtual control system (VCS) into the structure. A virtual control system is a self-balanced system, which consists of some control devices and a set of self-balanced forces. The self-balanced forces counterbalance the forces that the control devices apply on the structure. The control devices are combined with the structure to form a controlled structure used to replace the original structure in the substructure identification; and the self-balance forces are treated as known external excitations to the controlled structure. By optimally tuning the VCS’s parameters, the dynamic characteristics of the controlled structure can be changed such that the original structural responses become more favorable for the substructure identification and, thus, the identification accuracy is improved. A numerical example of 6-story shear structure is utilized to verify the effectiveness of the VCS based controlled substructure identification method. Finally, shake table tests are conducted on a 3-story structural model to verify the efficacy of the VCS to enhance the identification accuracy of the structural parameters.

  15. Evoking and Measuring Identification with Narrative Characters - A Linguistic Cues Framework.

    PubMed

    van Krieken, Kobie; Hoeken, Hans; Sanders, José

    2017-01-01

    Current research on identification with narrative characters poses two problems. First, although identification is seen as a dynamic process of which the intensity varies during reading, it is usually measured by means of post-reading questionnaires containing self-report items. Second, it is not clear which linguistic characteristics evoke identification. The present paper proposes that an interdisciplinary framework allows for more precise manipulations and measurements of identification, which will ultimately advance our understanding of the antecedents and nature of this process. The central hypothesis of our Linguistic Cues Framework is that identification with a narrative character is a multidimensional experience for which different dimensions are evoked by different linguistic cues. The first part of the paper presents a literature review on identification, resulting in a renewed conceptualization of identification which distinguishes six dimensions: a spatiotemporal, a perceptual, a cognitive, a moral, an emotional, and an embodied dimension. The second part argues that each of these dimensions is influenced by specific linguistic cues which represent various aspects of the narrative character's perspective. The proposed relations between linguistic cues and identification dimensions are specified in six propositions. The third part discusses what psychological and neurocognitive methods enable the measurement of the various identification dimensions in order to test the propositions. By establishing explicit connections between the linguistic characteristics of narratives and readers' physical, psychological, and neurocognitive responses to narratives, this paper develops a research agenda for future empirical research on identification with narrative characters.

  16. Cognitive Achievements in Identification Skills

    ERIC Educational Resources Information Center

    Randler, Christoph; Bogner, Franz X.

    2006-01-01

    Species identification tasks are generally accepted as fundamental aspects of biodiversity education. Our educational training unit, therefore, focused on identification skills by introducing stuffed specimens in combination with identification books and preparation booklets. We limited the number of bird species to six. 492 secondary school…

  17. Parental Identification by the Adolescent: A Social Power Approach

    ERIC Educational Resources Information Center

    McDonald, Gerald W.

    1977-01-01

    A social power theory of parental identification is presented, in contrast to sex-role theories of identification, which argues that the more parental power each parent is perceived to have, the higher the degree of adolescent identification with that parent. (Author)

  18. 48 CFR 252.211-7006 - Passive Radio Frequency Identification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... radio frequency identification (RFID) or item unique identification (IUID) information, order... CodeTM (EPC®) means an identification scheme for universally identifying physical objects via RFID tags... passive RFID technology. Exterior container means a MIL-STD-129 defined container, bundle, or assembly...

  19. 48 CFR 252.211-7006 - Passive Radio Frequency Identification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... radio frequency identification (RFID) or item unique identification (IUID) information, order... CodeTM (EPC®) means an identification scheme for universally identifying physical objects via RFID tags... passive RFID technology. Exterior container means a MIL-STD-129 defined container, bundle, or assembly...

  20. 48 CFR 252.211-7006 - Passive Radio Frequency Identification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... radio frequency identification (RFID) or item unique identification (IUID) information, order... CodeTM (EPC®) means an identification scheme for universally identifying physical objects via RFID tags... passive RFID technology. Exterior container means a MIL-STD-129 defined container, bundle, or assembly...

  1. Consistency of the Performance and Nonperformance Methods in Gifted Identification

    ERIC Educational Resources Information Center

    Acar, Selcuk; Sen, Sedat; Cayirdag, Nur

    2016-01-01

    Current approaches to gifted identification suggest collecting multiple sources of evidence. Some gifted identification guidelines allow for the interchangeable use of "performance" and "nonperformance" identification methods. This multiple criteria approach lacks a strong overlap between the assessment tools; however,…

  2. 7 CFR 29.133 - Identification number.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Identification number. 29.133 Section 29.133... REGULATIONS TOBACCO INSPECTION Regulations Miscellaneous § 29.133 Identification number. The Director may require the use of official identification numbers in connection with tobacco certificated or sampled...

  3. 7 CFR 29.133 - Identification number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Identification number. 29.133 Section 29.133... REGULATIONS TOBACCO INSPECTION Regulations Miscellaneous § 29.133 Identification number. The Director may require the use of official identification numbers in connection with tobacco certificated or sampled...

  4. 7 CFR 29.133 - Identification number.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Identification number. 29.133 Section 29.133... REGULATIONS TOBACCO INSPECTION Regulations Miscellaneous § 29.133 Identification number. The Director may require the use of official identification numbers in connection with tobacco certificated or sampled...

  5. 7 CFR 29.133 - Identification number.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Identification number. 29.133 Section 29.133... REGULATIONS TOBACCO INSPECTION Regulations Miscellaneous § 29.133 Identification number. The Director may require the use of official identification numbers in connection with tobacco certificated or sampled...

  6. 7 CFR 29.133 - Identification number.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Identification number. 29.133 Section 29.133... REGULATIONS TOBACCO INSPECTION Regulations Miscellaneous § 29.133 Identification number. The Director may require the use of official identification numbers in connection with tobacco certificated or sampled...

  7. 16 CFR 1633.11 - Records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... conditioning area before starting test, prototype or production identification number, and test data including.... For confirmation tests, the identification number must be that of the prototype tested. (2) Video and... prototype identification number or production lot identification number of the mattress set, date and time...

  8. MALDI-TOF MS versus VITEK 2 ANC card for identification of anaerobic bacteria.

    PubMed

    Li, Yang; Gu, Bing; Liu, Genyan; Xia, Wenying; Fan, Kun; Mei, Yaning; Huang, Peijun; Pan, Shiyang

    2014-05-01

    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an accurate, rapid and inexpensive technique that has initiated a revolution in the clinical microbiology laboratory for identification of pathogens. The Vitek 2 anaerobe and Corynebacterium (ANC) identification card is a newly developed method for identification of corynebacteria and anaerobic species. The aim of this study was to evaluate the effectiveness of the ANC card and MALDI-TOF MS techniques for identification of clinical anaerobic isolates. Five reference strains and a total of 50 anaerobic bacteria clinical isolates comprising ten different genera and 14 species were identified and analyzed by the ANC card together with Vitek 2 identification system and Vitek MS together with version 2.0 database respectively. 16S rRNA gene sequencing was used as reference method for accuracy in the identification. Vitek 2 ANC card and Vitek MS provided comparable results at species level for the five reference strains. Of 50 clinical strains, the Vitek MS provided identification for 46 strains (92%) to the species level, 47 (94%) to genus level, one (2%) low discrimination, two (4%) no identification and one (2%) misidentification. The Vitek 2 ANC card provided identification for 43 strains (86%) correct to the species level, 47 (94%) correct to the genus level, three (6%) low discrimination, three (6%) no identification and one (2%) misidentification. Both Vitek MS and Vitek 2 ANC card can be used for accurate routine clinical anaerobe identification. Comparing to the Vitek 2 ANC card, Vitek MS is easier, faster and more economic for each test. The databases currently available for both systems should be updated and further developed to enhance performance.

  9. Identification of blood culture isolates directly from positive blood cultures by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and a commercial extraction system: analysis of performance, cost, and turnaround time.

    PubMed

    Lagacé-Wiens, Philippe R S; Adam, Heather J; Karlowsky, James A; Nichol, Kimberly A; Pang, Paulette F; Guenther, Jodi; Webb, Amanda A; Miller, Crystal; Alfa, Michelle J

    2012-10-01

    Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry represents a revolution in the rapid identification of bacterial and fungal pathogens in the clinical microbiology laboratory. Recently, MALDI-TOF has been applied directly to positive blood culture bottles for the rapid identification of pathogens, leading to reductions in turnaround time and potentially beneficial patient impacts. The development of a commercially available extraction kit (Bruker Sepsityper) for use with the Bruker MALDI BioTyper has facilitated the processing required for identification of pathogens directly from positive from blood cultures. We report the results of an evaluation of the accuracy, cost, and turnaround time of this method for 61 positive monomicrobial and 2 polymicrobial cultures representing 26 species. The Bruker MALDI BioTyper with the Sepsityper gave a valid (score, >1.7) identification for 85.2% of positive blood cultures with no misidentifications. The mean reduction in turnaround time to identification was 34.3 h (P < 0.0001) in the ideal situation where MALDI-TOF was used for all blood cultures and 26.5 h in a more practical setting where conventional identification or identification from subcultures was required for isolates that could not be directly identified by MALDI-TOF. Implementation of a MALDI-TOF-based identification system for direct identification of pathogens from blood cultures is expected to be associated with a marginal increase in operating costs for most laboratories. However, the use of MALDI-TOF for direct identification is accurate and should result in reduced turnaround time to identification.

  10. Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry.

    PubMed

    La Scola, Bernard; Raoult, Didier

    2009-11-25

    With long delays observed between sampling and availability of results, the usefulness of blood cultures in the context of emergency infectious diseases has recently been questioned. Among methods that allow quicker bacterial identification from growing colonies, matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrometry was demonstrated to accurately identify bacteria routinely isolated in a clinical biology laboratory. In order to speed up the identification process, in the present work we attempted bacterial identification directly from blood culture bottles detected positive by the automate. We prospectively analysed routine MALDI-TOF identification of bacteria detected in blood culture by two different protocols involving successive centrifugations and then lysis by trifluoroacetic acid or formic acid. Of the 562 blood culture broths detected as positive by the automate and containing one bacterial species, 370 (66%) were correctly identified. Changing the protocol from trifluoroacetic acid to formic acid improved identification of Staphylococci, and overall correct identification increased from 59% to 76%. Lack of identification was observed mostly with viridans streptococci, and only one false positive was observed. In the 22 positive blood culture broths that contained two or more different species, only one of the species was identified in 18 samples, no species were identified in two samples and false species identifications were obtained in two cases. The positive predictive value of bacterial identification using this procedure was 99.2%. MALDI-TOF MS is an efficient method for direct routine identification of bacterial isolates in blood culture, with the exception of polymicrobial samples and viridans streptococci. It may replace routine identification performed on colonies, provided improvement for the specificity of blood culture broths growing viridans streptococci is obtained in the near future.

  11. Cognitive Impairment and Structural Abnormalities in Late Life Depression with Olfactory Identification Impairment: an Alzheimer's Disease-Like Pattern.

    PubMed

    Chen, Ben; Zhong, Xiaomei; Mai, Naikeng; Peng, Qi; Wu, Zhangying; Ouyang, Cong; Zhang, Weiru; Liang, Wanyuan; Wu, Yujie; Liu, Sha; Chen, Lijian; Ning, Yuping

    2018-03-15

    Late-life depression patients are at a high risk of developing Alzheimer's disease, and diminished olfactory identification is an indicator in early screening for Alzheimer's disease in the elderly. However, whether diminished olfactory identification is associated with risk of developing Alzheimer's disease in late-life depression patients remains unclear. One hundred and twenty-five late-life depression patients, 50 Alzheimer's disease patients, and 60 normal controls were continuously recruited. The participants underwent a clinical evaluation, olfactory test, neuropsychological assessment, and neuroimaging assessment. The olfactory identification impairment in late-life depression patients was milder than that in Alzheimer's disease patients. Diminished olfactory identification was significantly correlated with worse cognitive performance (global function, memory language, executive function, and attention) and reduced grey matter volume (olfactory bulb and hippocampus) in the late-life depression patients. According to a multiple linear regression analysis, olfactory identification was significantly associated with the memory scores in late-life depression group (B=1.623, P<.001). The late-life depression with olfactory identification impairment group had worse cognitive performance (global, memory, language, and executive function) and more structural abnormalities in Alzheimer's disease-related regions than the late-life depression without olfactory identification impairment group, and global cognitive function and logical memory in the late-life depression without olfactory identification impairment group was intact. Reduced volume observed in many areas (hippocampus, precuneus, etc.) in the Alzheimer's disease group was also observed in late-life depression with olfactory identification impairment group but not in the late-life depression without olfactory identification impairment group. The patterns of cognitive impairment and structural abnormalities in late-life depression with olfactory identification impairment patients were similar to those in Alzheimer's disease; olfactory identification may help identify late-life depression patients who are at a high risk of developing Alzheimer's disease.

  12. Teaching Bird Identification & Vocabulary with Twitter

    ERIC Educational Resources Information Center

    Hallman, Tyler A.; Robinson, W. Douglas

    2015-01-01

    Species identification is essential to biology, conservation, and management. The ability to focus on specific diagnostic characteristics of a species helps improve the speed and accuracy of identification. Birds are excellent subjects for teaching species identification because, in combination with their different shapes and sizes, their plumages…

  13. 48 CFR 652.237-71 - Identification/Building Pass.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Identification/Building... Identification/Building Pass. As prescribed in 637.110(b), insert the following clause. Identification/Building.... (1) The contractor shall obtain a Department of State building pass for all employees performing...

  14. 33 CFR 181.23 - Hull identification numbers required.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Hull identification numbers... SECURITY (CONTINUED) BOATING SAFETY MANUFACTURER REQUIREMENTS Identification of Boats § 181.23 Hull... identify each boat produced or imported with two hull identification numbers that meet the requirements of...

  15. 21 CFR 820.60 - Identification.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Identification. 820.60 Section 820.60 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES QUALITY SYSTEM REGULATION Identification and Traceability § 820.60 Identification. Each manufacturer shall...

  16. 33 CFR 401.20 - Automatic Identification System.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...' maritime Differential Global Positioning System radiobeacon services; or (7) The use of a temporary unit... Identification System. (a) Each of the following vessels must use an Automatic Identification System (AIS... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Automatic Identification System...

  17. 20 CFR 404.1220 - Identification numbers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Identification numbers. 404.1220 Section 404... § 404.1220 Identification numbers. (a) State and local governments. When a State submits a modification... will assign a special identification number to each political subdivision included in that modification...

  18. 20 CFR 404.1220 - Identification numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Identification numbers. 404.1220 Section 404... § 404.1220 Identification numbers. (a) State and local governments. When a State submits a modification... will assign a special identification number to each political subdivision included in that modification...

  19. 46 CFR 10.207 - Identification number.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 1 2011-10-01 2011-10-01 false Identification number. 10.207 Section 10.207 Shipping... CREDENTIAL General Requirements for All Merchant Mariner Credentials § 10.207 Identification number. For recordkeeping purposes only, a mariner's official MMC identification number is the individual's social security...

  20. 46 CFR 10.207 - Identification number.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 1 2013-10-01 2013-10-01 false Identification number. 10.207 Section 10.207 Shipping... CREDENTIAL General Requirements for All Merchant Mariner Credentials § 10.207 Identification number. For recordkeeping purposes only, a mariner's official MMC identification number is the individual's social security...

  1. 20 CFR 404.1220 - Identification numbers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false Identification numbers. 404.1220 Section 404... § 404.1220 Identification numbers. (a) State and local governments. When a State submits a modification... will assign a special identification number to each political subdivision included in that modification...

  2. 20 CFR 404.1220 - Identification numbers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Identification numbers. 404.1220 Section 404... § 404.1220 Identification numbers. (a) State and local governments. When a State submits a modification... will assign a special identification number to each political subdivision included in that modification...

  3. 46 CFR 10.207 - Identification number.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 1 2012-10-01 2012-10-01 false Identification number. 10.207 Section 10.207 Shipping... CREDENTIAL General Requirements for All Merchant Mariner Credentials § 10.207 Identification number. For recordkeeping purposes only, a mariner's official MMC identification number is the individual's social security...

  4. 46 CFR 10.207 - Identification number.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 1 2014-10-01 2014-10-01 false Identification number. 10.207 Section 10.207 Shipping... CREDENTIAL General Requirements for All Merchant Mariner Credentials § 10.207 Identification number. For recordkeeping purposes only, a mariner's official MMC identification number is the individual's social security...

  5. 75 FR 10414 - Researcher Identification Card

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... capturing administrative information on the characteristics of our users. Other forms of identification are... use bar-codes on researcher identification cards in the Washington, DC, area. The plastic cards we... plastic researcher identification cards as part of their security systems, we issue a plastic card to...

  6. 14 CFR 45.13 - Identification data.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Identification data. 45.13 Section 45.13 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT IDENTIFICATION AND REGISTRATION MARKING Marking of Products and Articles § 45.13 Identification data. Link to an...

  7. 42 CFR 493.1232 - Standard: Specimen identification and integrity.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Standard: Specimen identification and integrity... Nonwaived Testing General Laboratory Systems § 493.1232 Standard: Specimen identification and integrity. The laboratory must establish and follow written policies and procedures that ensure positive identification and...

  8. 14 CFR 45.14 - Identification of critical components.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Identification of critical components. 45.14 Section 45.14 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT IDENTIFICATION AND REGISTRATION MARKING Identification of Aircraft and Related Products § 45.14...

  9. 14 CFR 45.14 - Identification of critical components.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Identification of critical components. 45.14 Section 45.14 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT IDENTIFICATION AND REGISTRATION MARKING Marking of Products and Articles § 45.14 Identification of...

  10. Nuclear Magnetic Resonance Spectroscopy-Based Identification of Yeast.

    PubMed

    Himmelreich, Uwe; Sorrell, Tania C; Daniel, Heide-Marie

    2017-01-01

    Rapid and robust high-throughput identification of environmental, industrial, or clinical yeast isolates is important whenever relatively large numbers of samples need to be processed in a cost-efficient way. Nuclear magnetic resonance (NMR) spectroscopy generates complex data based on metabolite profiles, chemical composition and possibly on medium consumption, which can not only be used for the assessment of metabolic pathways but also for accurate identification of yeast down to the subspecies level. Initial results on NMR based yeast identification where comparable with conventional and DNA-based identification. Potential advantages of NMR spectroscopy in mycological laboratories include not only accurate identification but also the potential of automated sample delivery, automated analysis using computer-based methods, rapid turnaround time, high throughput, and low running costs.We describe here the sample preparation, data acquisition and analysis for NMR-based yeast identification. In addition, a roadmap for the development of classification strategies is given that will result in the acquisition of a database and analysis algorithms for yeast identification in different environments.

  11. A grass molecular identification system for forensic botany: a critical evaluation of the strengths and limitations.

    PubMed

    Ward, Jodie; Gilmore, Simon R; Robertson, James; Peakall, Rod

    2009-11-01

    Plant material is frequently encountered in criminal investigations but often overlooked as potential evidence. We designed a DNA-based molecular identification system for 100 Australian grasses that consisted of a series of polymerase chain reaction assays that enabled the progressive identification of grasses to different taxonomic levels. The identification system was based on DNA sequence variation at four chloroplast and two mitochondrial loci. Seventeen informative indels and 68 single-nucleotide polymorphisms were utilized as molecular markers for subfamily to species-level identification. To identify an unknown sample to subfamily level required a minimum of four markers or nine markers for species identification. The accuracy of the system was confirmed by blind tests. We have demonstrated "proof of concept" of a molecular identification system for trace botanical samples. Our evaluation suggests that the adoption of a system that combines this approach with DNA sequencing could assist the morphological identification of grasses found as forensic evidence.

  12. Olfactory identification deficit and its relationship with hedonic traits in patients with first-episode schizophrenia and individuals with schizotypy.

    PubMed

    Zou, Lai-Quan; Zhou, Han-Yu; Lui, Simon S Y; Wang, Yi; Wang, Ya; Gan, Jun; Zhu, Xiong-Zhao; Cheung, Eric F C; Chan, Raymond C K

    2018-04-20

    Olfactory identification impairments have been consistently found in schizophrenia patients. However, few previous studies have investigated this in first-episode patients. There are also inconsistent findings regarding olfactory identification ability in psychometrically-defined schizotypy individuals. In this study, we directly compared the olfactory identification ability of first-episode schizophrenia patients with schizotypy individuals. The relationship between olfactory identification impairments and hedonic traits was also examined. Thirty-five first-episode schizophrenia patients, 40 schizotypy individuals as defined by the Chapman's Anhedonia Scales and 40 demographically matched controls were recruited. The University of Pennsylvania Smell Identification Test was administered. Hedonic capacity was assessed using the Temporal Experience of Pleasure Scale (TEPS). The results showed that both the schizophrenia and schizotypy groups showed poorer olfactory identification ability than controls, and the impairment was significantly correlated with reduced pleasure experiences. Our findings support olfactory identification impairment as a trait marker for schizophrenia. Copyright © 2018 Elsevier Inc. All rights reserved.

  13. A Multiple Identity Approach to Gender: Identification with Women, Identification with Feminists, and Their Interaction

    PubMed Central

    van Breen, Jolien A.; Spears, Russell; Kuppens, Toon; de Lemus, Soledad

    2017-01-01

    Across four studies, we examine multiple identities in the context of gender and propose that women's attitudes toward gender group membership are governed by two largely orthogonal dimensions of gender identity: identification with women and identification with feminists. We argue that identification with women reflects attitudes toward the content society gives to group membership: what does it mean to be a woman in terms of group characteristics, interests and values? Identification with feminists, on the other hand, is a politicized identity dimension reflecting attitudes toward the social position of the group: what does it mean to be a woman in terms of disadvantage, inequality, and relative status? We examine the utility of this multiple identity approach in four studies. Study 1 showed that identification with women reflects attitudes toward group characteristics, such as femininity and self-stereotyping, while identification with feminists reflects attitudes toward the group's social position, such as perceived sexism. The two dimensions are shown to be largely independent, and as such provide support for the multiple identity approach. In Studies 2–4, we examine the utility of this multiple identity approach in predicting qualitative differences in gender attitudes. Results show that specific combinations of identification with women and feminists predicted attitudes toward collective action and gender stereotypes. Higher identification with feminists led to endorsement of radical collective action (Study 2) and critical attitudes toward gender stereotypes (Studies 3–4), especially at lower levels of identification with women. The different combinations of high vs. low identification with women and feminists can be thought of as reflecting four theoretical identity “types.” A woman can be (1) strongly identified with neither women nor feminists (“low identifier”), (2) strongly identified with women but less so with feminists (“traditional identifier”), (3) strongly identified with both women and feminists (“dual identifier”), or (4) strongly identified with feminists but less so with women (“distinctive feminist”). In sum, by considering identification with women and identification with feminists as multiple identities we aim to show how the multiple identity approach predicts distinct attitudes to gender issues and offer a new perspective on gender identity. PMID:28713297

  14. A Multiple Identity Approach to Gender: Identification with Women, Identification with Feminists, and Their Interaction.

    PubMed

    van Breen, Jolien A; Spears, Russell; Kuppens, Toon; de Lemus, Soledad

    2017-01-01

    Across four studies, we examine multiple identities in the context of gender and propose that women's attitudes toward gender group membership are governed by two largely orthogonal dimensions of gender identity: identification with women and identification with feminists. We argue that identification with women reflects attitudes toward the content society gives to group membership: what does it mean to be a woman in terms of group characteristics, interests and values? Identification with feminists, on the other hand, is a politicized identity dimension reflecting attitudes toward the social position of the group: what does it mean to be a woman in terms of disadvantage, inequality, and relative status? We examine the utility of this multiple identity approach in four studies. Study 1 showed that identification with women reflects attitudes toward group characteristics, such as femininity and self-stereotyping, while identification with feminists reflects attitudes toward the group's social position, such as perceived sexism. The two dimensions are shown to be largely independent, and as such provide support for the multiple identity approach. In Studies 2-4, we examine the utility of this multiple identity approach in predicting qualitative differences in gender attitudes. Results show that specific combinations of identification with women and feminists predicted attitudes toward collective action and gender stereotypes. Higher identification with feminists led to endorsement of radical collective action (Study 2) and critical attitudes toward gender stereotypes (Studies 3-4), especially at lower levels of identification with women. The different combinations of high vs. low identification with women and feminists can be thought of as reflecting four theoretical identity "types." A woman can be (1) strongly identified with neither women nor feminists ("low identifier"), (2) strongly identified with women but less so with feminists ("traditional identifier"), (3) strongly identified with both women and feminists ("dual identifier"), or (4) strongly identified with feminists but less so with women ("distinctive feminist"). In sum, by considering identification with women and identification with feminists as multiple identities we aim to show how the multiple identity approach predicts distinct attitudes to gender issues and offer a new perspective on gender identity.

  15. Algorithm Improvement Program Nuclide Identification Algorithm Scoring Criteria And Scoring Application - DNDO.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Enghauser, Michael

    2015-02-01

    The goal of the Domestic Nuclear Detection Office (DNDO) Algorithm Improvement Program (AIP) is to facilitate gamma-radiation detector nuclide identification algorithm development, improvement, and validation. Accordingly, scoring criteria have been developed to objectively assess the performance of nuclide identification algorithms. In addition, a Microsoft Excel spreadsheet application for automated nuclide identification scoring has been developed. This report provides an overview of the equations, nuclide weighting factors, nuclide equivalencies, and configuration weighting factors used by the application for scoring nuclide identification algorithm performance. Furthermore, this report presents a general overview of the nuclide identification algorithm scoring application including illustrative examples.

  16. Search-based model identification of smart-structure damage

    NASA Technical Reports Server (NTRS)

    Glass, B. J.; Macalou, A.

    1991-01-01

    This paper describes the use of a combined model and parameter identification approach, based on modal analysis and artificial intelligence (AI) techniques, for identifying damage or flaws in a rotating truss structure incorporating embedded piezoceramic sensors. This smart structure example is representative of a class of structures commonly found in aerospace systems and next generation space structures. Artificial intelligence techniques of classification, heuristic search, and an object-oriented knowledge base are used in an AI-based model identification approach. A finite model space is classified into a search tree, over which a variant of best-first search is used to identify the model whose stored response most closely matches that of the input. Newly-encountered models can be incorporated into the model space. This adaptativeness demonstrates the potential for learning control. Following this output-error model identification, numerical parameter identification is used to further refine the identified model. Given the rotating truss example in this paper, noisy data corresponding to various damage configurations are input to both this approach and a conventional parameter identification method. The combination of the AI-based model identification with parameter identification is shown to lead to smaller parameter corrections than required by the use of parameter identification alone.

  17. 40 CFR 262.12 - EPA identification numbers.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false EPA identification numbers. 262.12... (CONTINUED) STANDARDS APPLICABLE TO GENERATORS OF HAZARDOUS WASTE General § 262.12 EPA identification numbers... waste without having received an EPA identification number from the Administrator. (b) A generator who...

  18. 40 CFR 262.12 - EPA identification numbers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false EPA identification numbers. 262.12... (CONTINUED) STANDARDS APPLICABLE TO GENERATORS OF HAZARDOUS WASTE General § 262.12 EPA identification numbers... waste without having received an EPA identification number from the Administrator. (b) A generator who...

  19. 40 CFR 262.12 - EPA identification numbers.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false EPA identification numbers. 262.12... (CONTINUED) STANDARDS APPLICABLE TO GENERATORS OF HAZARDOUS WASTE General § 262.12 EPA identification numbers... waste without having received an EPA identification number from the Administrator. (b) A generator who...

  20. 40 CFR 262.12 - EPA identification numbers.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false EPA identification numbers. 262.12... (CONTINUED) STANDARDS APPLICABLE TO GENERATORS OF HAZARDOUS WASTE General § 262.12 EPA identification numbers... waste without having received an EPA identification number from the Administrator. (b) A generator who...

  1. 40 CFR 262.12 - EPA identification numbers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false EPA identification numbers. 262.12... (CONTINUED) STANDARDS APPLICABLE TO GENERATORS OF HAZARDOUS WASTE General § 262.12 EPA identification numbers... waste without having received an EPA identification number from the Administrator. (b) A generator who...

  2. Applying the Team Identification-Social Psychological Health Model to Older Sport Fans

    ERIC Educational Resources Information Center

    Wann, Daniel L.; Rogers, Kelly; Dooley, Keith; Foley, Mary

    2011-01-01

    According to the Team Identification-Social Psychological Health Model (Wann, 2006b), team identification and social psychological health should be positively correlated because identification leads to important social connections which, in turn, facilitate well-being. Although past research substantiates the hypothesized positive relationship…

  3. 17 CFR 270.0-11 - Customer identification programs.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Customer identification... (CONTINUED) RULES AND REGULATIONS, INVESTMENT COMPANY ACT OF 1940 § 270.0-11 Customer identification programs... implementing regulation at 31 CFR 103.131, which requires a customer identification program to be implemented...

  4. Learning Disability Identification Consistency: The Impact of Methodology and Student Evaluation Data

    ERIC Educational Resources Information Center

    Maki, Kathrin E.; Burns, Matthew K.; Sullivan, Amanda

    2017-01-01

    Learning disability (LD) identification has long been controversial and has undergone substantive reform. This study examined the consistency of school psychologists' LD identification decisions across three identification methods and across student evaluation data conclusiveness levels. Data were collected from 376 practicing school psychologists…

  5. 19 CFR 192.2 - Requirements for exportation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... documentation describing the vehicle, which includes the Vehicle Identification Number or, if the vehicle does not have a Vehicle Identification Number, the product identification number. Exportation of a vehicle... Identification Number (VIN), the name of the owner or lienholder of the leased vehicle, and the telephone numbers...

  6. 40 CFR 86.414-78 - Submission of vehicle identification number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 19 2014-07-01 2014-07-01 false Submission of vehicle identification... vehicle identification number. (a) Upon request by the Administrator, the manufacturer of any motorcycle covered by a certificate of conformity shall, within 30 days, identify by vehicle identification number...

  7. 19 CFR 192.2 - Requirements for exportation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... documentation describing the vehicle, which includes the Vehicle Identification Number or, if the vehicle does not have a Vehicle Identification Number, the product identification number. Exportation of a vehicle... Identification Number (VIN), the name of the owner or lienholder of the leased vehicle, and the telephone numbers...

  8. 77 FR 17105 - Identification of Interstate Motor Vehicles: City of Chicago, IL Registration Emblem Requirement...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-23

    ...-0086] Identification of Interstate Motor Vehicles: City of Chicago, IL Registration Emblem Requirement...) requesting a determination that the Commercial Motor Vehicle (CMV) identification requirement imposed by the... categories of excepted requirements. The first includes identification requirements related to motor vehicle...

  9. 19 CFR 192.2 - Requirements for exportation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... documentation describing the vehicle, which includes the Vehicle Identification Number or, if the vehicle does not have a Vehicle Identification Number, the product identification number. Exportation of a vehicle... Identification Number (VIN), the name of the owner or lienholder of the leased vehicle, and the telephone numbers...

  10. 40 CFR 86.414-78 - Submission of vehicle identification number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 19 2013-07-01 2013-07-01 false Submission of vehicle identification... vehicle identification number. (a) Upon request by the Administrator, the manufacturer of any motorcycle covered by a certificate of conformity shall, within 30 days, identify by vehicle identification number...

  11. 40 CFR 86.414-78 - Submission of vehicle identification number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 19 2012-07-01 2012-07-01 false Submission of vehicle identification... vehicle identification number. (a) Upon request by the Administrator, the manufacturer of any motorcycle covered by a certificate of conformity shall, within 30 days, identify by vehicle identification number...

  12. 40 CFR 86.414-78 - Submission of vehicle identification number.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 18 2011-07-01 2011-07-01 false Submission of vehicle identification... vehicle identification number. (a) Upon request by the Administrator, the manufacturer of any motorcycle covered by a certificate of conformity shall, within 30 days, identify by vehicle identification number...

  13. 40 CFR 86.414-78 - Submission of vehicle identification number.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 18 2010-07-01 2010-07-01 false Submission of vehicle identification... vehicle identification number. (a) Upon request by the Administrator, the manufacturer of any motorcycle covered by a certificate of conformity shall, within 30 days, identify by vehicle identification number...

  14. 41 CFR 101-25.110 - Tire identification/registration program.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Tire identification...-General Policies § 101-25.110 Tire identification/registration program. The regulations issued by the Department of Transportation in 49 CFR part 574, Tire Identification and Recordkeeping, require that tire...

  15. 78 FR 19311 - Certain Radio Frequency Identification (“RFID”) Products And Components Thereof; Institution of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-29

    ... Identification (``RFID'') Products And Components Thereof; Institution of Investigation Pursuant to 19 U.S.C... sale within the United States after importation of certain radio frequency identification (``RFID... after importation of certain radio frequency identification (``RFID'') products and components thereof...

  16. 27 CFR 19.242 - Employer identification number.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... number. 19.242 Section 19.242 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE... for Employer Identification Numbers § 19.242 Employer identification number. The proprietor must enter the employer identification number (EIN) assigned to it by the Internal Revenue Service on each form...

  17. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  18. 49 CFR 172.338 - Replacement of identification numbers.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 2 2013-10-01 2013-10-01 false Replacement of identification numbers. 172.338..., TRAINING REQUIREMENTS, AND SECURITY PLANS Marking § 172.338 Replacement of identification numbers. If more than one of the identification number markings on placards, orange panels, or white square-on-point...

  19. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  20. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  1. 49 CFR 172.338 - Replacement of identification numbers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Replacement of identification numbers. 172.338..., TRAINING REQUIREMENTS, AND SECURITY PLANS Marking § 172.338 Replacement of identification numbers. If more than one of the identification number markings on placards, orange panels, or white square-on-point...

  2. 49 CFR 172.338 - Replacement of identification numbers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 2 2011-10-01 2011-10-01 false Replacement of identification numbers. 172.338..., TRAINING REQUIREMENTS, AND SECURITY PLANS Marking § 172.338 Replacement of identification numbers. If more than one of the identification number markings on placards, orange panels, or white square-on-point...

  3. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  4. 40 CFR 265.11 - Identification number.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Identification number. 265.11 Section... FACILITIES General Facility Standards § 265.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR...

  5. 75 FR 29991 - Schedules for Atlantic Shark Identification Workshops and Protected Species Safe Handling...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-28

    ... certificate in order to fish with or renew their limited-access shark and limited-access swordfish permits... Atlantic Shark Identification Workshops and Protected Species Safe Handling, Release, and Identification... (NOAA), Commerce. ACTION: Notice of public workshops. SUMMARY: Free Atlantic Shark Identification...

  6. Comparison between MALDI-TOF MS and FilmArray Blood Culture Identification panel for rapid identification of yeast from positive blood culture.

    PubMed

    Paolucci, M; Foschi, C; Tamburini, M V; Ambretti, S; Lazzarotto, T; Landini, M P

    2014-09-01

    In this study we evaluated MALDI-TOF MS and FilmArray methods for the rapid identification of yeast from positive blood cultures. FilmArray correctly identified 20/22 of yeast species, while MALDI-TOF MS identified 9/22. FilmArray is a reliable and rapid identification system for the direct identification of yeasts from positive blood cultures. Copyright © 2014 Elsevier B.V. All rights reserved.

  7. Emerging new strategies for successful metabolite identification in metabolomics

    PubMed Central

    Bingol, Kerem; Bruschweiler-Li, Lei; Li, Dawei; Zhang, Bo; Xie, Mouzhe; Brüschweiler, Rafael

    2016-01-01

    This review discusses strategies for the identification of metabolites in complex biological mixtures, as encountered in metabolomics, which have emerged in the recent past. These include NMR database-assisted approaches for the identification of commonly known metabolites as well as novel combinations of NMR and MS analysis methods for the identification of unknown metabolites. The use of certain chemical additives to the NMR tube can permit identification of metabolites with specific physical chemical properties. PMID:26915807

  8. Decoupling Identification for Serial Two-Link Two-Inertia System

    NASA Astrophysics Data System (ADS)

    Oaki, Junji; Adachi, Shuichi

    The purpose of our study is to develop a precise model by applying the technique of system identification for the model-based control of a nonlinear robot arm, under taking joint-elasticity into consideration. We previously proposed a systematic identification method, called “decoupling identification,” for a “SCARA-type” planar two-link robot arm with elastic joints caused by the Harmonic-drive® reduction gears. The proposed method serves as an extension of the conventional rigid-joint-model-based identification. The robot arm is treated as a serial two-link two-inertia system with nonlinearity. The decoupling identification method using link-accelerometer signals enables the serial two-link two-inertia system to be divided into two linear one-link two-inertia systems. The MATLAB®'s commands for state-space model estimation are utilized in the proposed method. Physical parameters such as motor inertias, link inertias, joint-friction coefficients, and joint-spring coefficients are estimated through the identified one-link two-inertia systems using a gray-box approach. This paper describes accuracy evaluations using the two-link arm for the decoupling identification method under introducing closed-loop-controlled elements and varying amplitude-setup of identification-input. Experimental results show that the identification method also works with closed-loop-controlled elements. Therefore, the identification method is applicable to a “PUMA-type” vertical robot arm under gravity.

  9. Construction, implementation and testing of an image identification system using computer vision methods for fruit flies with economic importance (Diptera: Tephritidae).

    PubMed

    Wang, Jiang-Ning; Chen, Xiao-Lin; Hou, Xin-Wen; Zhou, Li-Bing; Zhu, Chao-Dong; Ji, Li-Qiang

    2017-07-01

    Many species of Tephritidae are damaging to fruit, which might negatively impact international fruit trade. Automatic or semi-automatic identification of fruit flies are greatly needed for diagnosing causes of damage and quarantine protocols for economically relevant insects. A fruit fly image identification system named AFIS1.0 has been developed using 74 species belonging to six genera, which include the majority of pests in the Tephritidae. The system combines automated image identification and manual verification, balancing operability and accuracy. AFIS1.0 integrates image analysis and expert system into a content-based image retrieval framework. In the the automatic identification module, AFIS1.0 gives candidate identification results. Afterwards users can do manual selection based on comparing unidentified images with a subset of images corresponding to the automatic identification result. The system uses Gabor surface features in automated identification and yielded an overall classification success rate of 87% to the species level by Independent Multi-part Image Automatic Identification Test. The system is useful for users with or without specific expertise on Tephritidae in the task of rapid and effective identification of fruit flies. It makes the application of computer vision technology to fruit fly recognition much closer to production level. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  10. Flavour identification in frontotemporal lobar degeneration.

    PubMed

    Omar, Rohani; Mahoney, Colin J; Buckley, Aisling H; Warren, Jason D

    2013-01-01

    Deficits of flavour processing may be clinically important in frontotemporal lobar degeneration (FTLD). To examine flavour processing in FTLD. We studied flavour identification prospectively in 25 patients with FTLD (12 with behavioural variant frontotemporal dementia (bvFTD), eight with semantic variant primary progressive aphasia (svPPA), five with non-fluent variant primary progressive aphasia (nfvPPA)) and 17 healthy control subjects, using a new test based on cross-modal matching of flavours to words and pictures. All subjects completed a general neuropsychological assessment, and odour identification was also assessed using a modified University of Pennsylvania Smell Identification Test. Brain MRI volumes from the patient cohort were analysed using voxel-based morphometry to identify regional grey matter associations of flavour identification. Relative to the healthy control group, the bvFTD and svPPA subgroups showed significant (p<0.05) deficits of flavour identification and all three FTLD subgroups showed deficits of odour identification. Flavour identification performance did not differ significantly between the FTLD syndromic subgroups. Flavour identification performance in the combined FTLD cohort was significantly (p<0.05 after multiple comparisons correction) associated with grey matter volume in the left entorhinal cortex, hippocampus, parahippocampal gyrus and temporal pole. Certain FTLD syndromes are associated with impaired flavour identification and this is underpinned by grey matter atrophy in an anteromedial temporal lobe network. These findings may have implications for our understanding of abnormal eating behaviour in these diseases.

  11. Applications of graph theory in protein structure identification

    PubMed Central

    2011-01-01

    There is a growing interest in the identification of proteins on the proteome wide scale. Among different kinds of protein structure identification methods, graph-theoretic methods are very sharp ones. Due to their lower costs, higher effectiveness and many other advantages, they have drawn more and more researchers’ attention nowadays. Specifically, graph-theoretic methods have been widely used in homology identification, side-chain cluster identification, peptide sequencing and so on. This paper reviews several methods in solving protein structure identification problems using graph theory. We mainly introduce classical methods and mathematical models including homology modeling based on clique finding, identification of side-chain clusters in protein structures upon graph spectrum, and de novo peptide sequencing via tandem mass spectrometry using the spectrum graph model. In addition, concluding remarks and future priorities of each method are given. PMID:22165974

  12. [Study on the automatic parameters identification of water pipe network model].

    PubMed

    Jia, Hai-Feng; Zhao, Qi-Feng

    2010-01-01

    Based on the problems analysis on development and application of water pipe network model, the model parameters automatic identification is regarded as a kernel bottleneck of model's application in water supply enterprise. The methodology of water pipe network model parameters automatic identification based on GIS and SCADA database is proposed. Then the kernel algorithm of model parameters automatic identification is studied, RSA (Regionalized Sensitivity Analysis) is used for automatic recognition of sensitive parameters, and MCS (Monte-Carlo Sampling) is used for automatic identification of parameters, the detail technical route based on RSA and MCS is presented. The module of water pipe network model parameters automatic identification is developed. At last, selected a typical water pipe network as a case, the case study on water pipe network model parameters automatic identification is conducted and the satisfied results are achieved.

  13. Patient identification error among prostate needle core biopsy specimens--are we ready for a DNA time-out?

    PubMed

    Suba, Eric J; Pfeifer, John D; Raab, Stephen S

    2007-10-01

    Patient identification errors in surgical pathology often involve switches of prostate or breast needle core biopsy specimens among patients. We assessed strategies for decreasing the occurrence of these uncommon and yet potentially catastrophic events. Root cause analyses were performed following 3 cases of patient identification error involving prostate needle core biopsy specimens. Patient identification errors in surgical pathology result from slips and lapses of automatic human action that may occur at numerous steps during pre-laboratory, laboratory and post-laboratory work flow processes. Patient identification errors among prostate needle biopsies may be difficult to entirely prevent through the optimization of work flow processes. A DNA time-out, whereby DNA polymorphic microsatellite analysis is used to confirm patient identification before radiation therapy or radical surgery, may eliminate patient identification errors among needle biopsies.

  14. Eyewitness confidence in simultaneous and sequential lineups: a criterion shift account for sequential mistaken identification overconfidence.

    PubMed

    Dobolyi, David G; Dodson, Chad S

    2013-12-01

    Confidence judgments for eyewitness identifications play an integral role in determining guilt during legal proceedings. Past research has shown that confidence in positive identifications is strongly associated with accuracy. Using a standard lineup recognition paradigm, we investigated accuracy using signal detection and ROC analyses, along with the tendency to choose a face with both simultaneous and sequential lineups. We replicated past findings of reduced rates of choosing with sequential as compared to simultaneous lineups, but notably found an accuracy advantage in favor of simultaneous lineups. Moreover, our analysis of the confidence-accuracy relationship revealed two key findings. First, we observed a sequential mistaken identification overconfidence effect: despite an overall reduction in false alarms, confidence for false alarms that did occur was higher with sequential lineups than with simultaneous lineups, with no differences in confidence for correct identifications. This sequential mistaken identification overconfidence effect is an expected byproduct of the use of a more conservative identification criterion with sequential than with simultaneous lineups. Second, we found a steady drop in confidence for mistaken identifications (i.e., foil identifications and false alarms) from the first to the last face in sequential lineups, whereas confidence in and accuracy of correct identifications remained relatively stable. Overall, we observed that sequential lineups are both less accurate and produce higher confidence false identifications than do simultaneous lineups. Given the increasing prominence of sequential lineups in our legal system, our data argue for increased scrutiny and possibly a wholesale reevaluation of this lineup format. PsycINFO Database Record (c) 2013 APA, all rights reserved.

  15. Ethnic Identification and School Language of Russian-Speaking Students in Estonia

    ERIC Educational Resources Information Center

    Kemppainen, Raija P.; Hilton, Sterling C.; Rannut, Ülle

    2015-01-01

    Ethnic identification is closely tied to language. Society's appreciation of one's first language and the opportunity to use it may help strengthen ethnic identification. This research examined the relationship between ethnic identifications and school language and investigated other factors that potentially impact language-minority students'…

  16. 9 CFR 590.800 - Identification of restricted eggs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Identification of restricted eggs. 590... AGRICULTURE EGG PRODUCTS INSPECTION INSPECTION OF EGGS AND EGG PRODUCTS (EGG PRODUCTS INSPECTION ACT) Identification of Restricted Eggs Or Egg Products Not Intended for Human Consumption § 590.800 Identification of...

  17. 24 CFR 232.616 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Social Security and Employer Identification Numbers. 232.616 Section 232.616 Housing and Urban... Borrowers § 232.616 Disclosure and verification of Social Security and Employer Identification Numbers. To... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  18. 24 CFR 200.6 - Employer identification and social security numbers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Employer identification and social... identification and social security numbers. The requirements set forth in 24 CFR part 5, regarding the disclosure and verification of social security numbers and employer identification numbers by applicants and...

  19. 24 CFR 201.6 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Social Security and Employer Identification Numbers. 201.6 Section 201.6 Housing and Urban Development... HOME LOANS General § 201.6 Disclosure and verification of Social Security and Employer Identification... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  20. 50 CFR 665.804 - Gear identification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Gear identification. 665.804 Section 665... Fisheries § 665.804 Gear identification. (a) Identification. The operator of each permitted vessel in the... action. Longline gear not marked in compliance with paragraph (a) of this section and found deployed in...

  1. 41 CFR 102-34.155 - What are the types of motor vehicle identification exemptions?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... motor vehicle identification exemptions? 102-34.155 Section 102-34.155 Public Contracts and Property... PROPERTY 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Identification Exemptions § 102-34.155 What are the types of motor vehicle identification exemptions? The types of motor vehicle...

  2. 40 CFR 86.079-36 - Submission of vehicle identification numbers.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 19 2012-07-01 2012-07-01 false Submission of vehicle identification... identification numbers. (a) Upon request of the Administrator, the manufacturer of any light-duty vehicle or... identification number, the vehicle(s) covered by the certificate of conformity. (b) The manufacturer of any light...

  3. 41 CFR 102-34.85 - What motor vehicles require motor vehicle identification?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... require motor vehicle identification? 102-34.85 Section 102-34.85 Public Contracts and Property Management... 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.85 What motor vehicles require motor vehicle identification? All Government motor vehicles must...

  4. 41 CFR 102-34.155 - What are the types of motor vehicle identification exemptions?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... motor vehicle identification exemptions? 102-34.155 Section 102-34.155 Public Contracts and Property... PROPERTY 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Identification Exemptions § 102-34.155 What are the types of motor vehicle identification exemptions? The types of motor vehicle...

  5. 40 CFR 86.079-36 - Submission of vehicle identification numbers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 18 2010-07-01 2010-07-01 false Submission of vehicle identification... identification numbers. (a) Upon request of the Administrator, the manufacturer of any light-duty vehicle or... identification number, the vehicle(s) covered by the certificate of conformity. (b) The manufacturer of any light...

  6. 49 CFR Appendix D to Part 512 - Vehicle Identification Number Information

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 6 2013-10-01 2013-10-01 false Vehicle Identification Number Information D.... 512, App. D Appendix D to Part 512—Vehicle Identification Number Information The Chief Counsel has...) characters, of vehicle identification numbers reported in information on incidents involving death or injury...

  7. 41 CFR 102-34.155 - What are the types of motor vehicle identification exemptions?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... motor vehicle identification exemptions? 102-34.155 Section 102-34.155 Public Contracts and Property... PROPERTY 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Identification Exemptions § 102-34.155 What are the types of motor vehicle identification exemptions? The types of motor vehicle...

  8. 40 CFR 86.079-36 - Submission of vehicle identification numbers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 18 2011-07-01 2011-07-01 false Submission of vehicle identification... identification numbers. (a) Upon request of the Administrator, the manufacturer of any light-duty vehicle or... identification number, the vehicle(s) covered by the certificate of conformity. (b) The manufacturer of any light...

  9. 41 CFR 102-34.155 - What are the types of motor vehicle identification exemptions?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... motor vehicle identification exemptions? 102-34.155 Section 102-34.155 Public Contracts and Property... PROPERTY 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Identification Exemptions § 102-34.155 What are the types of motor vehicle identification exemptions? The types of motor vehicle...

  10. 41 CFR 102-34.85 - What motor vehicles require motor vehicle identification?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... require motor vehicle identification? 102-34.85 Section 102-34.85 Public Contracts and Property Management... 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.85 What motor vehicles require motor vehicle identification? All Government motor vehicles must...

  11. 49 CFR Appendix D to Part 512 - Vehicle Identification Number Information

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 6 2011-10-01 2011-10-01 false Vehicle Identification Number Information D.... 512, App. D Appendix D to Part 512—Vehicle Identification Number Information The Chief Counsel has...) characters, of vehicle identification numbers reported in information on incidents involving death or injury...

  12. 41 CFR 102-34.155 - What are the types of motor vehicle identification exemptions?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... motor vehicle identification exemptions? 102-34.155 Section 102-34.155 Public Contracts and Property... PROPERTY 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Identification Exemptions § 102-34.155 What are the types of motor vehicle identification exemptions? The types of motor vehicle...

  13. 49 CFR Appendix D to Part 512 - Vehicle Identification Number Information

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 6 2014-10-01 2014-10-01 false Vehicle Identification Number Information D.... 512, App. D Appendix D to Part 512—Vehicle Identification Number Information The Chief Counsel has...) characters, of vehicle identification numbers reported in information on incidents involving death or injury...

  14. 40 CFR 86.079-36 - Submission of vehicle identification numbers.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 19 2013-07-01 2013-07-01 false Submission of vehicle identification... identification numbers. (a) Upon request of the Administrator, the manufacturer of any light-duty vehicle or... identification number, the vehicle(s) covered by the certificate of conformity. (b) The manufacturer of any light...

  15. 49 CFR Appendix D to Part 512 - Vehicle Identification Number Information

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 6 2012-10-01 2012-10-01 false Vehicle Identification Number Information D.... 512, App. D Appendix D to Part 512—Vehicle Identification Number Information The Chief Counsel has...) characters, of vehicle identification numbers reported in information on incidents involving death or injury...

  16. 41 CFR 102-34.85 - What motor vehicles require motor vehicle identification?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... require motor vehicle identification? 102-34.85 Section 102-34.85 Public Contracts and Property Management... 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.85 What motor vehicles require motor vehicle identification? All Government motor vehicles must...

  17. Identification of pilot-vehicle dynamics from simulation and flight test

    NASA Technical Reports Server (NTRS)

    Hess, Ronald A.

    1990-01-01

    The paper discusses an identification problem in which a basic feedback control structure, or pilot control strategy, is hypothesized. Identification algorithms are employed to determine the particular form of pilot equalization in each feedback loop. It was found that both frequency- and time-domain identification techniques provide useful information.

  18. 41 CFR 102-34.85 - What motor vehicles require motor vehicle identification?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... require motor vehicle identification? 102-34.85 Section 102-34.85 Public Contracts and Property Management... 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.85 What motor vehicles require motor vehicle identification? All Government motor vehicles must...

  19. 41 CFR 102-34.85 - What motor vehicles require motor vehicle identification?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... require motor vehicle identification? 102-34.85 Section 102-34.85 Public Contracts and Property Management... 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification § 102-34.85 What motor vehicles require motor vehicle identification? All Government motor vehicles must...

  20. 47 CFR 80.231 - Technical Requirements for Class B Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Identification System (AIS) equipment. 80.231 Section 80.231 Telecommunication FEDERAL COMMUNICATIONS COMMISSION... § 80.231 Technical Requirements for Class B Automatic Identification System (AIS) equipment. (a) Class B Automatic Identification System (AIS) equipment must meet the technical requirements of IEC 62287...

  1. 47 CFR 80.231 - Technical Requirements for Class B Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Identification System (AIS) equipment. 80.231 Section 80.231 Telecommunication FEDERAL COMMUNICATIONS COMMISSION... § 80.231 Technical Requirements for Class B Automatic Identification System (AIS) equipment. (a) Class B Automatic Identification System (AIS) equipment must meet the technical requirements of IEC 62287...

  2. 47 CFR 80.231 - Technical Requirements for Class B Automatic Identification System (AIS) equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Identification System (AIS) equipment. 80.231 Section 80.231 Telecommunication FEDERAL COMMUNICATIONS COMMISSION... § 80.231 Technical Requirements for Class B Automatic Identification System (AIS) equipment. (a) Class B Automatic Identification System (AIS) equipment must meet the technical requirements of IEC 62287...

  3. 24 CFR 232.616 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Social Security and Employer Identification Numbers. 232.616 Section 232.616 Housing and Urban... Borrowers § 232.616 Disclosure and verification of Social Security and Employer Identification Numbers. To... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  4. 24 CFR 201.6 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Social Security and Employer Identification Numbers. 201.6 Section 201.6 Housing and Urban Development... HOME LOANS General § 201.6 Disclosure and verification of Social Security and Employer Identification... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  5. 24 CFR 200.6 - Employer identification and social security numbers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Employer identification and social... identification and social security numbers. The requirements set forth in 24 CFR part 5, regarding the disclosure and verification of social security numbers and employer identification numbers by applicants and...

  6. 24 CFR 200.6 - Employer identification and social security numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Employer identification and social... identification and social security numbers. The requirements set forth in 24 CFR part 5, regarding the disclosure and verification of social security numbers and employer identification numbers by applicants and...

  7. 24 CFR 232.616 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Social Security and Employer Identification Numbers. 232.616 Section 232.616 Housing and Urban... Borrowers § 232.616 Disclosure and verification of Social Security and Employer Identification Numbers. To... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  8. 24 CFR 200.6 - Employer identification and social security numbers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Employer identification and social... identification and social security numbers. The requirements set forth in 24 CFR part 5, regarding the disclosure and verification of social security numbers and employer identification numbers by applicants and...

  9. 24 CFR 201.6 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Social Security and Employer Identification Numbers. 201.6 Section 201.6 Housing and Urban Development... HOME LOANS General § 201.6 Disclosure and verification of Social Security and Employer Identification... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  10. 24 CFR 232.616 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Social Security and Employer Identification Numbers. 232.616 Section 232.616 Housing and Urban... Borrowers § 232.616 Disclosure and verification of Social Security and Employer Identification Numbers. To... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  11. 24 CFR 201.6 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Social Security and Employer Identification Numbers. 201.6 Section 201.6 Housing and Urban Development... HOME LOANS General § 201.6 Disclosure and verification of Social Security and Employer Identification... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  12. 24 CFR 200.6 - Employer identification and social security numbers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Employer identification and social... identification and social security numbers. The requirements set forth in 24 CFR part 5, regarding the disclosure and verification of social security numbers and employer identification numbers by applicants and...

  13. 24 CFR 232.616 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Social Security and Employer Identification Numbers. 232.616 Section 232.616 Housing and Urban... Borrowers § 232.616 Disclosure and verification of Social Security and Employer Identification Numbers. To... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  14. 24 CFR 201.6 - Disclosure and verification of Social Security and Employer Identification Numbers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Social Security and Employer Identification Numbers. 201.6 Section 201.6 Housing and Urban Development... HOME LOANS General § 201.6 Disclosure and verification of Social Security and Employer Identification... the disclosure and verification of Social Security and Employer Identification Numbers, as provided by...

  15. 47 CFR 95.119 - Station identification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... station identification is the call sign assigned to the GMRS station or system. (c) A unit number may be... 47 Telecommunication 5 2013-10-01 2013-10-01 false Station identification. 95.119 Section 95.119... SERVICES General Mobile Radio Service (GMRS) § 95.119 Station identification. (a) Except as provided in...

  16. 47 CFR 95.119 - Station identification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... station identification is the call sign assigned to the GMRS station or system. (c) A unit number may be... 47 Telecommunication 5 2012-10-01 2012-10-01 false Station identification. 95.119 Section 95.119... SERVICES General Mobile Radio Service (GMRS) § 95.119 Station identification. (a) Except as provided in...

  17. 47 CFR 95.119 - Station identification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... station identification is the call sign assigned to the GMRS station or system. (c) A unit number may be... 47 Telecommunication 5 2011-10-01 2011-10-01 false Station identification. 95.119 Section 95.119... SERVICES General Mobile Radio Service (GMRS) § 95.119 Station identification. (a) Except as provided in...

  18. 47 CFR 95.119 - Station identification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... station identification is the call sign assigned to the GMRS station or system. (c) A unit number may be... 47 Telecommunication 5 2014-10-01 2014-10-01 false Station identification. 95.119 Section 95.119... SERVICES General Mobile Radio Service (GMRS) § 95.119 Station identification. (a) Except as provided in...

  19. 48 CFR 552.211-92 - Radio Frequency Identification (RFID) using passive tags.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Identification (RFID) using passive tags. 552.211-92 Section 552.211-92 Federal Acquisition Regulations System... Provisions and Clauses 552.211-92 Radio Frequency Identification (RFID) using passive tags. As prescribed in 511.204(b)(11), insert the following clause: Radio Frequency Identification (RFID) Using Passive Tags...

  20. 48 CFR 552.211-92 - Radio Frequency Identification (RFID) using passive tags.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Identification (RFID) using passive tags. 552.211-92 Section 552.211-92 Federal Acquisition Regulations System... Provisions and Clauses 552.211-92 Radio Frequency Identification (RFID) using passive tags. As prescribed in 511.204(b)(11), insert the following clause: Radio Frequency Identification (RFID) Using Passive Tags...

  1. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  2. 7 CFR 75.48 - Identification number.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Identification number. 75.48 Section 75.48 Agriculture... number. The Director may require the use of official identification numbers in connection with seed certificated or sampled under the Act. When identification numbers are required, they shall be specified by the...

  3. 7 CFR 75.48 - Identification number.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Identification number. 75.48 Section 75.48 Agriculture... number. The Director may require the use of official identification numbers in connection with seed certificated or sampled under the Act. When identification numbers are required, they shall be specified by the...

  4. 20 CFR 422.112 - Employer identification numbers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Employer identification numbers. 422.112... Procedures § 422.112 Employer identification numbers. (a) General. Most employers are required by section...)-1 to obtain an employer identification number (EIN) and to include it on wage reports filed with SSA...

  5. 16 CFR 301.26 - Registered identification numbers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Registered identification numbers. 301.26... numbers. (a) Registered numbers for use as the required identification in lieu of the name on fur product... (d) of this section. (b)(1) Registered identification numbers shall be used only by the person or...

  6. 20 CFR 422.112 - Employer identification numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Employer identification numbers. 422.112... Procedures § 422.112 Employer identification numbers. (a) General. Most employers are required by section...)-1 to obtain an employer identification number (EIN) and to include it on wage reports filed with SSA...

  7. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 27 2012-07-01 2012-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  8. 33 CFR 181.23 - Hull identification numbers required.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Hull identification numbers... identification numbers required. (a) A manufacturer must identify each boat produced or imported with primary and secondary hull identification numbers permanently affixed in accordance with § 181.29 of this subpart. (b) A...

  9. 26 CFR 31.6011(b)-1 - Employers' identification numbers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 15 2013-04-01 2013-04-01 false Employers' identification numbers. 31.6011(b)-1... Subtitle F, Internal Revenue Code of 1954) § 31.6011(b)-1 Employers' identification numbers. (a... Insurance Contributions Act, but who prior to such day neither has been assigned an identification number...

  10. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 26 2014-07-01 2014-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  11. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 27 2013-07-01 2013-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  12. 33 CFR 181.23 - Hull identification numbers required.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Hull identification numbers... identification numbers required. (a) A manufacturer must identify each boat produced or imported with primary and secondary hull identification numbers permanently affixed in accordance with § 181.29 of this subpart. (b) A...

  13. 7 CFR 75.48 - Identification number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Identification number. 75.48 Section 75.48 Agriculture... number. The Director may require the use of official identification numbers in connection with seed certificated or sampled under the Act. When identification numbers are required, they shall be specified by the...

  14. 7 CFR 75.48 - Identification number.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Identification number. 75.48 Section 75.48 Agriculture... number. The Director may require the use of official identification numbers in connection with seed certificated or sampled under the Act. When identification numbers are required, they shall be specified by the...

  15. 7 CFR 75.48 - Identification number.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Identification number. 75.48 Section 75.48 Agriculture... number. The Director may require the use of official identification numbers in connection with seed certificated or sampled under the Act. When identification numbers are required, they shall be specified by the...

  16. 40 CFR 264.11 - Identification number.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 26 2011-07-01 2011-07-01 false Identification number. 264.11 Section... Facility Standards § 264.11 Identification number. Every facility owner or operator must apply to EPA for an EPA identification number in accordance with the EPA notification procedures (45 FR 12746). [45 FR...

  17. 26 CFR 31.6011(b)-1 - Employers' identification numbers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 26 Internal Revenue 15 2014-04-01 2014-04-01 false Employers' identification numbers. 31.6011(b)-1... Subtitle F, Internal Revenue Code of 1954) § 31.6011(b)-1 Employers' identification numbers. (a... Insurance Contributions Act, but who prior to such day neither has been assigned an identification number...

  18. 21 CFR 830.310 - Information required for unique device identification.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Information required for unique device identification. 830.310 Section 830.310 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND... Identification Database § 830.310 Information required for unique device identification. The contact for device...

  19. 9 CFR 79.2 - Identification of sheep and goats in interstate commerce.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... prefix that has been linked in the National Scrapie Database with the assigned premises identification... official identification method or device approved by the Administrator. (3) The owner of the flock of... premises identification if they are linked to the premises in the National Scrapie Database) will be...

  20. 9 CFR 79.2 - Identification of sheep and goats in interstate commerce.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... prefix that has been linked in the National Scrapie Database with the assigned premises identification... official identification method or device approved by the Administrator. (3) The owner of the flock of... premises identification if they are linked to the premises in the National Scrapie Database) will be...

  1. 9 CFR 79.2 - Identification of sheep and goats in interstate commerce.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... prefix that has been linked in the National Scrapie Database with the assigned premises identification... official identification method or device approved by the Administrator. (3) The owner of the flock of... premises identification if they are linked to the premises in the National Scrapie Database) will be...

  2. 9 CFR 590.800 - Identification of restricted eggs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Identification of restricted eggs. 590... AGRICULTURE EGG PRODUCTS INSPECTION INSPECTION OF EGGS AND EGG PRODUCTS (EGG PRODUCTS INSPECTION ACT) Identification of Restricted Eggs Or Egg Products Not Intended for Human Consumption § 590.800 Identification of...

  3. Collaborative identification method for sea battlefield target based on deep convolutional neural networks

    NASA Astrophysics Data System (ADS)

    Zheng, Guangdi; Pan, Mingbo; Liu, Wei; Wu, Xuetong

    2018-03-01

    The target identification of the sea battlefield is the prerequisite for the judgment of the enemy in the modern naval battle. In this paper, a collaborative identification method based on convolution neural network is proposed to identify the typical targets of sea battlefields. Different from the traditional single-input/single-output identification method, the proposed method constructs a multi-input/single-output co-identification architecture based on optimized convolution neural network and weighted D-S evidence theory. The simulation results show that

  4. An overview of recent advances in system identification

    NASA Technical Reports Server (NTRS)

    Juang, Jer-Nan

    1994-01-01

    This paper presents an overview of the recent advances in system identification for modal testing and control of large flexible structures. Several techniques are discussed including the Observer/Kalman Filter Identification, the Observer/Controller Identification, and the State-Space System Identification in the Frequency Domain. The System/Observer/Controller Toolbox developed at NASA Langley Research Center is used to show the applications of these techniques to real aerospace structures such as the Hubble spacecraft telescope and the active flexible aircraft wing.

  5. Comparison of traditional phenotypic identification methods with partial 5' 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli.

    PubMed

    Cloud, Joann L; Harmsen, Dag; Iwen, Peter C; Dunn, James J; Hall, Gerri; Lasala, Paul Rocco; Hoggan, Karen; Wilson, Deborah; Woods, Gail L; Mellmann, Alexander

    2010-04-01

    Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5' 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.

  6. Software For Computer-Security Audits

    NASA Technical Reports Server (NTRS)

    Arndt, Kate; Lonsford, Emily

    1994-01-01

    Information relevant to potential breaches of security gathered efficiently. Automated Auditing Tools for VAX/VMS program includes following automated software tools performing noted tasks: Privileged ID Identification, program identifies users and their privileges to circumvent existing computer security measures; Critical File Protection, critical files not properly protected identified; Inactive ID Identification, identifications of users no longer in use found; Password Lifetime Review, maximum lifetimes of passwords of all identifications determined; and Password Length Review, minimum allowed length of passwords of all identifications determined. Written in DEC VAX DCL language.

  7. Using the domain identification model to study major and career decision-making processes

    NASA Astrophysics Data System (ADS)

    Tendhar, Chosang; Singh, Kusum; Jones, Brett D.

    2018-03-01

    The purpose of this study was to examine the extent to which (1) a domain identification model could be used to predict students' engineering major and career intentions and (2) the MUSIC Model of Motivation components could be used to predict domain identification. The data for this study were collected from first-year engineering students. We used a structural equation model to test the hypothesised relationship between variables in the partial domain identification model. The findings suggested that engineering identification significantly predicted engineering major intentions and career intentions and had the highest effect on those two variables compared to other motivational constructs. Furthermore, results suggested that success, interest, and caring are plausible contributors to students' engineering identification. Overall, there is strong evidence that the domain identification model can be used as a lens to study career decision-making processes in engineering, and potentially, in other fields as well.

  8. A visual identification key utilizing both gestalt and analytic approaches to identification of Carices present in North America (Plantae, Cyperaceae)

    PubMed Central

    2013-01-01

    Abstract Images are a critical part of the identification process because they enable direct, immediate and relatively unmediated comparisons between a specimen being identified and one or more reference specimens. The Carices Interactive Visual Identification Key (CIVIK) is a novel tool for identification of North American Carex species, the largest vascular plant genus in North America, and two less numerous closely-related genera, Cymophyllus and Kobresia. CIVIK incorporates 1288 high-resolution tiled image sets that allow users to zoom in to view minute structures that are crucial at times for identification in these genera. Morphological data are derived from the earlier Carex Interactive Identification Key (CIIK) which in turn used data from the Flora of North America treatments. In this new iteration, images can be viewed in a grid or histogram format, allowing multiple representations of data. In both formats the images are fully zoomable. PMID:24723777

  9. Multiple social identifications and adolescents' self-esteem.

    PubMed

    Benish-Weisman, Maya; Daniel, Ella; Schiefer, David; Möllering, Anna; Knafo-Noam, Ariel

    2015-10-01

    The research examined the relationship between multiple social identifications and self-esteem. Early adolescents (M = 11.4, SD = .95) and mid-adolescents (M = 15.9, SD = 1.18) from Germany and Israel (n = 2337) were studied. Respondents described their social identification as students, family members, and as members of the majority national group and reported self-esteem. A longitudinal, cross-sectional and cross-cultural design revealed, as predicted, multiple social identifications related positively to self-esteem concurrently; they also related positively to self-esteem longitudinally over the course of a year. Moreover, multiple social identifications were found to be antecedent to self-esteem, not vice versa. Finally, multiple social identifications were found to decrease over time. The article discusses the contribution of multiple social identifications to self-esteem at different ages and in various contexts. Copyright © 2015 The Foundation for Professionals in Services for Adolescents. Published by Elsevier Ltd. All rights reserved.

  10. A rapid, one step molecular identification of Trichoderma citrinoviride and Trichoderma reesei.

    PubMed

    Saroj, Dina B; Dengeti, Shrinivas N; Aher, Supriya; Gupta, Anil K

    2015-06-01

    Trichoderma species are widely used as production hosts for industrial enzymes. Identification of Trichoderma species requires a complex molecular biology based identification involving amplification and sequencing of multiple genes. Industrial laboratories are required to run identification tests repeatedly in cell banking procedures and also to prove absence of production host in the product. Such demands can be fulfilled by a brief method which enables confirmation of strain identity. This communication describes one step identification method for two common Trichoderma species; T. citrinoviride and T. reesei, based on identification of polymorphic region in the nucleotide sequence of translation elongation factor 1 alpha. A unique forward primer and common reverse primer resulted in 153 and 139 bp amplicon for T. citrinoviride and T. reesei, respectively. Simplification was further introduced by using mycelium as template for PCR amplification. Method described in this communication allows rapid, one step identification of two Trichoderma species.

  11. 77 FR 51811 - Draft Guidance for Industry on Self-Identification of Generic Drug Facilities, Sites, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-27

    ... as it prepares to meet the self- identification requirement. It explains who is required to self... describing how FDA will implement the self- identification requirement contained in GDUFA. As required by... industry as it prepares to meet the self-identification requirement. The guidance explains who is required...

  12. Model Identification in Time-Series Analysis: Some Empirical Results.

    ERIC Educational Resources Information Center

    Padia, William L.

    Model identification of time-series data is essential to valid statistical tests of intervention effects. Model identification is, at best, inexact in the social and behavioral sciences where one is often confronted with small numbers of observations. These problems are discussed, and the results of independent identifications of 130 social and…

  13. 76 FR 79155 - International Affairs; Identification of Nations Whose Fishing Vessels Are Engaged in Fishing in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-21

    ..., taking into account different conditions. The Secretary is required to begin making identifications not...: On March 24, 2011, NMFS published a notice and requested information regarding nations whose vessels... making identifications by January 4, 2012, and publish the first identifications in the January 2013...

  14. Children's Subjective Identification with Social Groups: A Self-Stereotyping Approach

    ERIC Educational Resources Information Center

    Bennett, Mark; Sani, Fabio

    2008-01-01

    We contend that previous work on children's identification with social groups has looked at the mere categorization of the self in group terms and not subjective identification properly conceived. Drawing upon self-categorization theory, the present research operationalizes identification as self-stereotyping (i.e. the ability to conceive of the…

  15. Eyewitness Identification Accuracy and Response Latency: The Unruly 10-12-Second Rule

    ERIC Educational Resources Information Center

    Weber, Nathan; Brewer, Neil; Wells, Gary L.; Semmler, Carolyn; Keast, Amber

    2004-01-01

    Data are reported from 3,213 research eyewitnesses confirming that accurate eyewitness identifications from lineups are made faster than are inaccurate identifications. However, consistent with predictions from the recognition and search literatures, the authors did not find support for the "10-12-s rule" in which lineup identifications faster…

  16. 50 CFR 660.319 - Open access fishery gear identification and marking.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false Open access fishery gear identification... COAST STATES West Coast Groundfish-Open Access Fisheries § 660.319 Open access fishery gear identification and marking. (a) Gear identification. (1) Open access fixed gear (longline, trap or pot, set net...

  17. 21 CFR 830.350 - Correction of information submitted to the Global Unique Device Identification Database.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Unique Device Identification Database. 830.350 Section 830.350 Food and Drugs FOOD AND DRUG... Global Unique Device Identification Database § 830.350 Correction of information submitted to the Global Unique Device Identification Database. (a) If FDA becomes aware that any information submitted to the...

  18. Chapter 01: Wood identification and pattern recognition

    Treesearch

    Alex Wiedenhoeft

    2011-01-01

    Wood identification is a combination of art and science. Although the bulk of this manual focuses on the scientific characteristics used to make accurate field identifications of wood, the contribution of the artistic component to the identification process should be neither overlooked nor understated. Though the accumulation of scientific knowledge and experience is...

  19. 21 CFR 801.57 - Discontinuation of legacy FDA identification numbers assigned to devices.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Discontinuation of legacy FDA identification... Device Identification § 801.57 Discontinuation of legacy FDA identification numbers assigned to devices... been assigned an FDA labeler code to facilitate use of NHRIC or NDC numbers may continue to use that...

  20. Disentangling the Roles of Institutional and Individual Poverty in the Identification of Gifted Students

    ERIC Educational Resources Information Center

    Hamilton, Rashea; McCoach, D. Betsy; Tutwiler, M. Shane; Siegle, Del; Gubbins, E. Jean; Callahan, Carolyn M.; Brodersen, Annalissa V.; Mun, Rachel U.

    2018-01-01

    Although the relationships between family income and student identification for gifted programming are well documented, less is known about how school and district wealth are related to student identification. To examine the effects of institutional and individual poverty on student identification, we conducted a series of three-level regression…

  1. Content Analysis of Measures for Identification of Elder Abuse.

    ERIC Educational Resources Information Center

    Sengstock, Mary C.; And Others

    Measures designed to detect elder abuse lack uniformity as a result of having been designed in isolation. To develop and test a uniform index for the identification of elder abuse victims, an analysis of existing abuse identification instruments was conducted. Initially, seven elder abuse identification measures were content analyzed, resulting in…

  2. 21 CFR 830.300 - Devices subject to device identification data submission requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Devices subject to device identification data submission requirements. 830.300 Section 830.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF... Identification Database § 830.300 Devices subject to device identification data submission requirements. (a) In...

  3. 21 CFR 830.330 - Times for submission of unique device identification information.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Times for submission of unique device identification information. 830.330 Section 830.330 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF... Identification Database § 830.330 Times for submission of unique device identification information. (a) The...

  4. When the ends outweigh the means: mood and level of identification in depression.

    PubMed

    Watkins, Edward R; Moberly, Nicholas J; Moulds, Michelle L

    2011-11-01

    Research in healthy controls has found that mood influences cognitive processing via level of action identification: happy moods are associated with global and abstract processing; sad moods are associated with local and concrete processing. However, this pattern seems inconsistent with the high level of abstract processing observed in depressed patients, leading Watkins (2008, 2010) to hypothesise that the association between mood and level of goal/action identification is impaired in depression. We tested this hypothesis by measuring level of identification on the Behavioural Identification Form after happy and sad mood inductions in never-depressed controls and currently depressed patients. Participants used increasingly concrete action identifications as they became sadder and less happy, but this effect was moderated by depression status. Consistent with Watkins' (2008) hypothesis, increases in sad mood and decreases in happiness were associated with shifts towards the use of more concrete action identifications in never-depressed individuals, but not in depressed patients. These findings suggest that the putatively adaptive association between mood and level of identification is impaired in major depression.

  5. Identification with groups: the role of personality and context.

    PubMed

    Sagiv, Lilach; Roccas, Sonia; Hazan, Osnat

    2012-04-01

    Is identification a product of personality or of the context? We examine this question by adopting a multidimensional conceptualization of identification (the CIDS model) that integrates research perspectives on personality and contextual effects. We investigate (Study 1) the relationships of traits to identification with the nation (students, N = 77), the army (soldiers, N = 220), and a business school (students, N = 123). Then we show that the modes of identification vary in their stability across social contexts and in their susceptibility to contextual change. Idealizing groups' symbols ("deference" identification) is especially stable across different foci of identification (Study 2): the military and former high school (soldiers, N = 188), the business school and the nation (students, = 62), and the military and one's ethnic group (soldiers, N = 95). Perceiving the group as a central part of the self ("importance" identification) is the most susceptible to contextual effects of priming values (Study 3; students, N = 80, 60) and the group's status (Study 4; students, N = 68). © 2011 The Authors. Journal of Personality © 2012, Wiley Periodicals, Inc.

  6. Identification and its vicissitudes.

    PubMed

    Etchegoyen, R H

    1985-01-01

    This paper attempts to understand the vicissitudes of identification within the co-ordinates of narcissism and the object relation. Firstly the dialectic pair primary identification/secondary identification are studied, and primary narcissism is suggested as the hypothesis which best explains them. The complex identification processes in the primary scene are considered next and the importance of the introjection of the oedipal parents for the formation of the superego is underlined. The importance of the structuring function of the introjection and projection mechanisms becomes embodied in the concept of projective identification, which comes to question the postulate of primary narcissism. The theory of projective-introjective identification is an extremely powerful instrument for explaining phenomena, however it obliges one to accept that the first introjections are radically different from the others. They have nothing to do with mourning but rather with primitive mechanisms which question the subject/object polarity and, so this author believes, spring basically from envy. Lastly, it is maintained that envy and libido are factors of a dialectic from which the object relation and the earliest processes of identification, previous to the Oedipus complex, proceed at one and the same time.

  7. On the optimal identification of tag sets in time-constrained RFID configurations.

    PubMed

    Vales-Alonso, Javier; Bueno-Delgado, María Victoria; Egea-López, Esteban; Alcaraz, Juan José; Pérez-Mañogil, Juan Manuel

    2011-01-01

    In Radio Frequency Identification facilities the identification delay of a set of tags is mainly caused by the random access nature of the reading protocol, yielding a random identification time of the set of tags. In this paper, the cumulative distribution function of the identification time is evaluated using a discrete time Markov chain for single-set time-constrained passive RFID systems, namely those ones where a single group of tags is assumed to be in the reading area and only for a bounded time (sojourn time) before leaving. In these scenarios some tags in a set may leave the reader coverage area unidentified. The probability of this event is obtained from the cumulative distribution function of the identification time as a function of the sojourn time. This result provides a suitable criterion to minimize the probability of losing tags. Besides, an identification strategy based on splitting the set of tags in smaller subsets is also considered. Results demonstrate that there are optimal splitting configurations that reduce the overall identification time while keeping the same probability of losing tags.

  8. Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy.

    PubMed

    Li, X Y; Xu, H X; Chen, J W

    2014-04-29

    Manual cultivar identification diagram is a new strategy for plant cultivar identification based on DNA markers, providing information to efficiently separate cultivars. We tested 25 pairs of apple EST-SSR primers for amplification of PCR products from loquat cultivars. These EST-SSR primers provided clear amplification products from the loquat cultivars, with a relatively high transferability rate of 84% to loquat; 11 pairs of primers amplified polymorphic products. After analysis of 24 red-fleshed loquat accessions, we found that only 7 pairs of primers could clearly separate all of them. A cultivar identification diagram of the 24 cultivars was constructed using polymorphic bands from the DNA fingerprints and EST-SSR primers. Any two of the 24 cultivars could be rapidly separated from each other, according to the polymorphic bands from the cultivars; the corresponding primers were marked in the correct position on the cultivar identification diagram. This red-flesh loquat cultivar identification diagram can separate the 24 red-flesh loquat cultivars, which is of benefit for loquat cultivar identification for germplasm management and breeding programs.

  9. When the ends outweigh the means: Mood and level of identification in depression

    PubMed Central

    Watkins, Edward R.; Moberly, Nicholas J.; Moulds, Michelle L.

    2011-01-01

    Research in healthy controls has found that mood influences cognitive processing via level of action identification: happy moods are associated with global and abstract processing; sad moods are associated with local and concrete processing. However, this pattern seems inconsistent with the high level of abstract processing observed in depressed patients, leading Watkins (2008, 2010) to hypothesise that the association between mood and level of goal/action identification is impaired in depression. We tested this hypothesis by measuring level of identification on the Behavioural Identification Form after happy and sad mood inductions in never-depressed controls and currently depressed patients. Participants used increasingly concrete action identifications as they became sadder and less happy, but this effect was moderated by depression status. Consistent with Watkins' (2008) hypothesis, increases in sad mood and decreases in happiness were associated with shifts towards the use of more concrete action identifications in never-depressed individuals, but not in depressed patients. These findings suggest that the putatively adaptive association between mood and level of identification is impaired in major depression. PMID:22017614

  10. A between-subjects test of the lower-identification/ higher-priming paradox.

    PubMed

    Rubino, I Alex; Rociola, Giuseppe; Di Lorenzo, Giorgio; Magni, Valentina; Ribolsi, Michele; Mancini, Valentina; Saya, Anna; Pezzarossa, Bianca; Siracusano, Alberto; Suslow, Thomas

    2013-01-01

    An under-recognised U-shaped model states that unconscious and conscious perceptual effects are functionally exclusive and that unconscious perceptual effects manifest themselves only at the objective detection threshold, when conscious perception is completely absent. We tested the U-shaped line model with a between-subjects paradigm. Angry, happy, neutral faces, or blank slides were flashed for 5.5 ms and 19.5 ms before Chinese ideographs in a darkened room. A group of volunteers (n = 84) were asked to rate how much they liked each ideograph and performed an identification task. According to the median identification score two subgroups were composed; one with 50% or < 50% identification scores (n = 31), and one with above 50% identification scores (n = 53). The hypothesised U-shaped line was confirmed by the findings. Affective priming was found only at the two extreme points: the 5.5 ms condition of the low-identification group (subliminal perception) and the 19.5 ms condition of the > 50% high-identification group (supraliminal perception). The two intermediate points (19.5 ms of the low-identification group and 5.5 ms of the high-identification group) did not correspond to significant priming effects. These results confirm that a complete absence of conscious perception is the condition for the deployment of unconscious perceptual effects.

  11. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification.

    PubMed

    Bizzini, A; Greub, G

    2010-11-01

    Until recently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) techniques for the identification of microorganisms remained confined to research laboratories. In the last 2 years, the availability of relatively simple to use MALDI-TOF MS devices, which can be utilized in clinical microbiology laboratories, has changed the laboratory workflows for the identification of pathogens. Recently, the first prospective studies regarding the performance in routine bacterial identification showed that MALDI-TOF MS is a fast, reliable and cost-effective technique that has the potential to replace and/or complement conventional phenotypic identification for most bacterial strains isolated in clinical microbiology laboratories. For routine bacterial isolates, correct identification by MALDI-TOF MS at the species level was obtained in 84.1-93.6% of instances. In one of these studies, a protein extraction step clearly improved the overall valid identification yield, from 70.3% to 93.2%. This review focuses on the current state of use of MALDI-TOF MS for the identification of routine bacterial isolates and on the main difficulties that may lead to erroneous or doubtful identifications. © 2010 The Authors. Clinical Microbiology and Infection © 2010 European Society of Clinical Microbiology and Infectious Diseases.

  12. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for the identification of clinical filamentous fungi.

    PubMed

    Huang, Yanfei; Zhang, Mingxin; Zhu, Min; Wang, Mei; Sun, Yufeng; Gu, Haitong; Cao, Jingjing; Li, Xue; Zhang, Shaoya; Wang, Jinglin; Lu, Xinxin

    2017-07-01

    Infections caused by filamentous fungi have become a health concern, and require rapid and accurate identification in order for effective treatment of the pathogens. To compare the performance of two MALDI-TOF MS systems (Bruker Microflex LT and Xiamen Microtyper) in the identification of filamentous fungal species. A total of 374 clinical filamentous fungal isolates sequentially collected in the Clinical Laboratory at the Beijing Tongren Hospital between January 2014 and December 2015 were identified by traditional phenotypic methods, Bruker Microflex LT and Xiamen Microtyper MALDI-TOF MS, respectively. The discrepancy between these methods was resolved by sequencing for definitive identification. Bruker Microflex LT and Xiamen Microtyper had similar correct species ID (98.9 vs. 99.2%), genus ID (99.7 vs. 100%), mis-ID (0.3 vs. 0%) and no ID (0 vs. 0). The rate of correct species identification by both MALDI-TOF MS (98.9 and 99.2%, respectively) was much higher compared with phenotypic approach (91.9%). Both MALDI-TOF MS systems provide accurate identification of clinical filamentous fungi compared with conventional phenotypic method, and have the potential to replace identification for routine identification of these fungi in clinical mycology laboratories. Both systems have similar performance in the identification of clinical filamentous fungi.

  13. Predicting and interpreting identification errors in military vehicle training using multidimensional scaling.

    PubMed

    Bohil, Corey J; Higgins, Nicholas A; Keebler, Joseph R

    2014-01-01

    We compared methods for predicting and understanding the source of confusion errors during military vehicle identification training. Participants completed training to identify main battle tanks. They also completed card-sorting and similarity-rating tasks to express their mental representation of resemblance across the set of training items. We expected participants to selectively attend to a subset of vehicle features during these tasks, and we hypothesised that we could predict identification confusion errors based on the outcomes of the card-sort and similarity-rating tasks. Based on card-sorting results, we were able to predict about 45% of observed identification confusions. Based on multidimensional scaling of the similarity-rating data, we could predict more than 80% of identification confusions. These methods also enabled us to infer the dimensions receiving significant attention from each participant. This understanding of mental representation may be crucial in creating personalised training that directs attention to features that are critical for accurate identification. Participants completed military vehicle identification training and testing, along with card-sorting and similarity-rating tasks. The data enabled us to predict up to 84% of identification confusion errors and to understand the mental representation underlying these errors. These methods have potential to improve training and reduce identification errors leading to fratricide.

  14. Experimental parameter identification of a multi-scale musculoskeletal model controlled by electrical stimulation: application to patients with spinal cord injury.

    PubMed

    Benoussaad, Mourad; Poignet, Philippe; Hayashibe, Mitsuhiro; Azevedo-Coste, Christine; Fattal, Charles; Guiraud, David

    2013-06-01

    We investigated the parameter identification of a multi-scale physiological model of skeletal muscle, based on Huxley's formulation. We focused particularly on the knee joint controlled by quadriceps muscles under electrical stimulation (ES) in subjects with a complete spinal cord injury. A noninvasive and in vivo identification protocol was thus applied through surface stimulation in nine subjects and through neural stimulation in one ES-implanted subject. The identification protocol included initial identification steps, which are adaptations of existing identification techniques to estimate most of the parameters of our model. Then we applied an original and safer identification protocol in dynamic conditions, which required resolution of a nonlinear programming (NLP) problem to identify the serial element stiffness of quadriceps. Each identification step and cross validation of the estimated model in dynamic condition were evaluated through a quadratic error criterion. The results highlighted good accuracy, the efficiency of the identification protocol and the ability of the estimated model to predict the subject-specific behavior of the musculoskeletal system. From the comparison of parameter values between subjects, we discussed and explored the inter-subject variability of parameters in order to select parameters that have to be identified in each patient.

  15. Odors identification differences in deficit and nondeficit schizophrenia.

    PubMed

    Pełka-Wysiecka, Justyna; Wroński, Michał; Bieńkowski, Przemysław; Murawiec, Sławomir; Samochowiec, Agnieszka; Samochowiec, Jerzy

    2016-04-01

    There is evidence that deficit schizophrenia (DS) is associated with neuroanatomical changes in structures including those involved in olfaction. Olfactory dysfunction, which includes impaired odor identification, is found in patients with schizophrenia and their family members. 82 patients with DS and 72 patients with NDS (nondeficit schizophrenia), somatically healthy and without acute psychotic symptoms undertook a smell identification test using the 16-item Sniffin' Sticks ID test. Demographic and psychometric data were collected. No differences in the course of the illness, perinatal history and demographic data were found between the DS and NDS groups. No differences in the number of correctly identified odor samples were found. Some differences in the qualitative identification of samples between DS and NDS were found in the groups of female (fewer correct identifications of cinnamon and pineapple smells in DS) and male patients (fewer correct identifications of the smell of rose and more correct identifications of the smell of orange than in NDS). No overall differences between DS and NDS regarding odors identification have been found. The results seem to indicate some specific deficits in the identification of markers of rose, pineapple, orange and cinnamon. Copyright © 2015 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

  16. Towards large-scale FAME-based bacterial species identification using machine learning techniques.

    PubMed

    Slabbinck, Bram; De Baets, Bernard; Dawyndt, Peter; De Vos, Paul

    2009-05-01

    In the last decade, bacterial taxonomy witnessed a huge expansion. The swift pace of bacterial species (re-)definitions has a serious impact on the accuracy and completeness of first-line identification methods. Consequently, back-end identification libraries need to be synchronized with the List of Prokaryotic names with Standing in Nomenclature. In this study, we focus on bacterial fatty acid methyl ester (FAME) profiling as a broadly used first-line identification method. From the BAME@LMG database, we have selected FAME profiles of individual strains belonging to the genera Bacillus, Paenibacillus and Pseudomonas. Only those profiles resulting from standard growth conditions have been retained. The corresponding data set covers 74, 44 and 95 validly published bacterial species, respectively, represented by 961, 378 and 1673 standard FAME profiles. Through the application of machine learning techniques in a supervised strategy, different computational models have been built for genus and species identification. Three techniques have been considered: artificial neural networks, random forests and support vector machines. Nearly perfect identification has been achieved at genus level. Notwithstanding the known limited discriminative power of FAME analysis for species identification, the computational models have resulted in good species identification results for the three genera. For Bacillus, Paenibacillus and Pseudomonas, random forests have resulted in sensitivity values, respectively, 0.847, 0.901 and 0.708. The random forests models outperform those of the other machine learning techniques. Moreover, our machine learning approach also outperformed the Sherlock MIS (MIDI Inc., Newark, DE, USA). These results show that machine learning proves very useful for FAME-based bacterial species identification. Besides good bacterial identification at species level, speed and ease of taxonomic synchronization are major advantages of this computational species identification strategy.

  17. Rapid Identification of Mycobacteria and Drug-Resistant Mycobacterium tuberculosis by Use of a Single Multiplex PCR and DNA Sequencing

    PubMed Central

    Pérez-Osorio, Ailyn C.; Boyle, David S.; Ingham, Zachary K.; Ostash, Alla; Gautom, Romesh K.; Colombel, Craig; Houze, Yolanda

    2012-01-01

    Tuberculosis (TB) remains a significant global health problem for which rapid diagnosis is critical to both treatment and control. This report describes a multiplex PCR method, the Mycobacterial IDentification and Drug Resistance Screen (MID-DRS) assay, which allows identification of members of the Mycobacterium tuberculosis complex (MTBC) and the simultaneous amplification of targets for sequencing-based drug resistance screening of rifampin-resistant (rifampinr), isoniazidr, and pyrazinamider TB. Additionally, the same multiplex reaction amplifies a specific 16S rRNA gene target for rapid identification of M. avium complex (MAC) and a region of the heat shock protein 65 gene (hsp65) for further DNA sequencing-based confirmation or identification of other mycobacterial species. Comparison of preliminary results generated with MID-DRS versus culture-based methods for a total of 188 bacterial isolates demonstrated MID-DRS sensitivity and specificity as 100% and 96.8% for MTBC identification; 100% and 98.3% for MAC identification; 97.4% and 98.7% for rifampinr TB identification; 60.6% and 100% for isoniazidr TB identification; and 75.0% and 98.1% for pyrazinamider TB identification. The performance of the MID-DRS was also tested on acid-fast-bacterium (AFB)-positive clinical specimens, resulting in sensitivity and specificity of 100% and 78.6% for detection of MTBC and 100% and 97.8% for detection of MAC. In conclusion, use of the MID-DRS reduces the time necessary for initial identification and drug resistance screening of TB specimens to as little as 2 days. Since all targets needed for completing the assay are included in a single PCR amplification step, assay costs, preparation time, and risks due to user errors are also reduced. PMID:22162548

  18. Bacterial rapid identification with matrix assisted laser desorption/ionization time-of-flight mass spectrometry: development of an 'in-house method' and comparison with Bruker Sepsityper(®) kit.

    PubMed

    Frédéric Ric, S; Antoine, M; Bodson, A; Lissoir, B

    2015-10-01

    The objective of this study was to compare an in-house matrix-assisted laser desorption ionization with time of flight (MALDI-TOF) method and a commercial MALDI-TOF kit (Sepsityper(®) kit) for direct bacterial identification in positive blood cultures. We also evaluated the time saved and the cost associated with the rapid identification techniques. We used the BACTEC(®) automated system for detecting positive blood cultures. Direct identification using Sepsityper kit and the in-house method were compared with conventional identification by MALDI-TOF using pure bacterial culture on the solid phase. We also evaluated different cut-off scores for rapid bacterial identification. In total, 127 positive blood vials were selected. The rate of rapid identification with the MALDI Sepsityper kit was 25.2% with the standard cut-off and 33.9% with the enlarged cut-off, while the results for the in-house method were 44.1 and 61.4%, respectively. Error rates with the enlarged cut-off were 6.98 (n = 3) and 2.56% (n = 2) for Sepsityper and the in-house method, respectively. Identification rates were higher for gram-negative bacteria. Direct bacterial identification succeeded in supplying rapid identification of the causative organism in cases of sepsis. The time taken to obtain a result was nearly 24  hours shorter for the direct bacterial identification methods than for conventional MALDI-TOF on solid phase culture. Compared with the Sepsityper kit, the in-house method offered better results and fewer errors, was more cost-effective and easier to use.

  19. Fine-tuned evaluation of olfactory function in patients operated for nasal polyposis.

    PubMed

    Sonnet, Marie-Hortense; Nguyen, Duc Trung; Nguyen-Thi, Phi-Linh; Arous, Fabien; Jankowski, Roger; Rumeau, Cécile

    2017-07-01

    Given the forced-choice procedure of the identification test, patients with profound anosmia are more likely to have higher identification scores by chance than patients with hyposmia or normosmia. This may be a confusing factor when assessing the sense of smell, which alters the appreciation of real olfaction improvement. The aim of this study was to fine-tune the results of the identification Sniffin' Sticks test before and 6 weeks after surgery using the real identification score. A total of 133 patients underwent the Identification (I) and Threshold (T) tests the day before and 6 weeks after nasalization surgery. The scores of the identification test, called I G (global identification), were ranked from 0 to 16. Patients had to specify if their forced-choice answers were given either surely or randomly, called I H (hazard identification). The real score of identification I R was obtained as follow: I R  = I G  - I H . Patients with an immeasurable threshold according to the T test were more prone to give randomly correct answers. On the basis of I G scores, 43.6% of patients remained hypo-anosmic after surgery compared to 72.9% before surgery. Using I R scores, only 3.8% of patients remained anosmic (I R  = 0) at 6 weeks after surgery. Hence, patients with real anosmia (I R  = 0) were less prone to improve their olfaction than patients with I R  > 0. The analysis of random factor when using identification test allows differentiating a real anosmia from a hyposmia. An I G  ≤ 4 could be considered as a profound/real anosmia or a severe hyposmia. This procedure cannot, however, replace the forced-choice method in odor identification testing.

  20. Optimizing identification of clinically relevant Gram-positive organisms by use of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system.

    PubMed

    McElvania Tekippe, Erin; Shuey, Sunni; Winkler, David W; Butler, Meghan A; Burnham, Carey-Ann D

    2013-05-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can be used as a method for the rapid identification of microorganisms. This study evaluated the Bruker Biotyper (MALDI-TOF MS) system for the identification of clinically relevant Gram-positive organisms. We tested 239 aerobic Gram-positive organisms isolated from clinical specimens. We evaluated 4 direct-smear methods, including "heavy" (H) and "light" (L) smears, with and without a 1-μl direct formic acid (FA) overlay. The quality measure assigned to a MALDI-TOF MS identification is a numerical value or "score." We found that a heavy smear with a formic acid overlay (H+FA) produced optimal MALDI-TOF MS identification scores and the highest percentage of correctly identified organisms. Using a score of ≥2.0, we identified 183 of the 239 isolates (76.6%) to the genus level, and of the 181 isolates resolved to the species level, 141 isolates (77.9%) were correctly identified. To maximize the number of correct identifications while minimizing misidentifications, the data were analyzed using a score of ≥1.7 for genus- and species-level identification. Using this score, 220 of the 239 isolates (92.1%) were identified to the genus level, and of the 181 isolates resolved to the species level, 167 isolates (92.2%) could be assigned an accurate species identification. We also evaluated a subset of isolates for preanalytic factors that might influence MALDI-TOF MS identification. Frequent subcultures increased the number of unidentified isolates. Incubation temperatures and subcultures of the media did not alter the rate of identification. These data define the ideal bacterial preparation, identification score, and medium conditions for optimal identification of Gram-positive bacteria by use of MALDI-TOF MS.

  1. Optimizing Identification of Clinically Relevant Gram-Positive Organisms by Use of the Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System

    PubMed Central

    McElvania TeKippe, Erin; Shuey, Sunni; Winkler, David W.; Butler, Meghan A.

    2013-01-01

    Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) can be used as a method for the rapid identification of microorganisms. This study evaluated the Bruker Biotyper (MALDI-TOF MS) system for the identification of clinically relevant Gram-positive organisms. We tested 239 aerobic Gram-positive organisms isolated from clinical specimens. We evaluated 4 direct-smear methods, including “heavy” (H) and “light” (L) smears, with and without a 1-μl direct formic acid (FA) overlay. The quality measure assigned to a MALDI-TOF MS identification is a numerical value or “score.” We found that a heavy smear with a formic acid overlay (H+FA) produced optimal MALDI-TOF MS identification scores and the highest percentage of correctly identified organisms. Using a score of ≥2.0, we identified 183 of the 239 isolates (76.6%) to the genus level, and of the 181 isolates resolved to the species level, 141 isolates (77.9%) were correctly identified. To maximize the number of correct identifications while minimizing misidentifications, the data were analyzed using a score of ≥1.7 for genus- and species-level identification. Using this score, 220 of the 239 isolates (92.1%) were identified to the genus level, and of the 181 isolates resolved to the species level, 167 isolates (92.2%) could be assigned an accurate species identification. We also evaluated a subset of isolates for preanalytic factors that might influence MALDI-TOF MS identification. Frequent subcultures increased the number of unidentified isolates. Incubation temperatures and subcultures of the media did not alter the rate of identification. These data define the ideal bacterial preparation, identification score, and medium conditions for optimal identification of Gram-positive bacteria by use of MALDI-TOF MS. PMID:23426925

  2. Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

    PubMed

    Cassagne, Carole; Ranque, Stéphane; Normand, Anne-Cécile; Fourquet, Patrick; Thiebault, Sandrine; Planard, Chantal; Hendrickx, Marijke; Piarroux, Renaud

    2011-01-01

    MALDI-TOF MS recently emerged as a valuable identification tool for bacteria and yeasts and revolutionized the daily clinical laboratory routine. But it has not been established for routine mould identification. This study aimed to validate a standardized procedure for MALDI-TOF MS-based mould identification in clinical laboratory. First, pre-extraction and extraction procedures were optimized. With this standardized procedure, a 143 mould strains reference spectra library was built. Then, the mould isolates cultured from sequential clinical samples were prospectively subjected to this MALDI-TOF MS based-identification assay. MALDI-TOF MS-based identification was considered correct if it was concordant with the phenotypic identification; otherwise, the gold standard was DNA sequence comparison-based identification. The optimized procedure comprised a culture on sabouraud-gentamicin-chloramphenicol agar followed by a chemical extraction of the fungal colonies with formic acid and acetonitril. The identification was done using a reference database built with references from at least four culture replicates. For five months, 197 clinical isolates were analyzed; 20 were excluded because they were not identified at the species level. MALDI-TOF MS-based approach correctly identified 87% (154/177) of the isolates analyzed in a routine clinical laboratory activity. It failed in 12% (21/177), whose species were not represented in the reference library. MALDI-TOF MS-based identification was correct in 154 out of the remaining 156 isolates. One Beauveria bassiana was not identified and one Rhizopus oryzae was misidentified as Mucor circinelloides. This work's seminal finding is that a standardized procedure can also be used for MALDI-TOF MS-based identification of a wide array of clinically relevant mould species. It thus makes it possible to identify moulds in the routine clinical laboratory setting and opens new avenues for the development of an integrated MALDI-TOF MS-based solution for the identification of any clinically relevant microorganism.

  3. On the reciprocal effects between multiple group identifications and mental health: A longitudinal study of Scottish adolescents.

    PubMed

    Miller, Kirsty; Wakefield, Juliet R H; Sani, Fabio

    2017-11-01

    The aim of the study was to investigate the link between social group identification and mental health outcomes in a sample of secondary school pupils. Based on previous work, it was predicted that multiple high group identifications would protect against psychological ill health. Furthermore, it was predicted that better mental health would also predict greater number of group identifications, thus creating a 'virtuous circle'. A longitudinal questionnaire design was used. A total of 409 Scottish secondary school pupils aged 13-17 completed a questionnaire twice over a year. Pupils' responses regarding their mental health and the extent of their identification with three groups (the family, school, and friends) were measured. A path analysis of the data showed that greater number of high group identifications predicted better mental health outcomes amongst participants. However, better mental health also predicted greater number of high group identifications, suggesting that there is a cyclical relationship between both variables. The findings have both theoretical and practical implications. They highlight the importance of conceptualizing the link between group identification and mental health as cyclical, rather than unidirectional. This reconceptualization has implications for mental health promotion strategies, as it highlights the importance of attempting to turn a potentially 'vicious cycle' of social disidentification and mental ill health into a 'virtuous cycle' of social identification and mental health. Results showed that in a population of 409 high school pupils, the more high group identifications pupils had, the better their mental health outcomes. Better mental health also predicted a greater number of high group identifications over time. The findings suggest that we would benefit from conceptualizing the relationship between group identification and mental outcomes as being cyclical rather than unidirectional. Viewing the relationship between group identification and mental health in this way enables us to consider interventions which help turn a 'vicious cycle' into a 'virtuous cycle'. Limitations A potential limitation of the work relates to the use of self-report questionnaires which may elicit socially desirable responses. The sample only consists of high school pupils from mainstream public schools within Scotland. © 2017 The British Psychological Society.

  4. 12 CFR Appendix D to Part 229 - Indorsement, Reconverting Bank Identification, and Truncating Bank Identification Standards

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 3 2011-01-01 2011-01-01 false Indorsement, Reconverting Bank Identification, and Truncating Bank Identification Standards D Appendix D to Part 229 Banks and Banking FEDERAL... COLLECTION OF CHECKS (REGULATION CC) Pt. 229, App. D Appendix D to Part 229—Indorsement, Reconverting Bank...

  5. Department Identification, Professional Identification, and Attitudes toward Agriculture in Agriculture College Students

    ERIC Educational Resources Information Center

    Yueh, Hsiu-Ping; Chen, Tzy-Ling; Cheng, Po-Jen

    2014-01-01

    The purpose of this study was to investigate the department identification, professional identification, and attitude toward agriculture of agriculture students. As the scope of agricultural higher education has been readjusted to focus on a system of content-related innovations instead of primary production, it is also an aim of the study to…

  6. Active Noise and Vibration Control Literature Survey: Controller Technologies

    DTIC Science & Technology

    1999-11-01

    5.4 Schematic Flowchart of System Identification [Soderstrom, 1989] ................. 5. 7 Measurement System (open-loop...approaches: measurement systems and transfer functions identification [Norton, 1986]. The following figure illustrates the general flowchart ...data SCI Figure 5.3. Schematic flowchart of system identification [Soderstrom, 1989] 5.7 The first type of identification {see Figure 5.4) uses open

  7. Books and Balls: Antecedents and Outcomes of College Identification

    ERIC Educational Resources Information Center

    Porter, Thomas; Hartman, Katherine; Johnson, John Seth

    2011-01-01

    Identification plays a central role in models of giving to an organization. This study presents and tests a general model of giving that highlights status based and affect based drivers of identification. The model was tested using a sample of 114 alumni from 74 different colleges participated in an online survey. Identification was found to…

  8. Community-Based Efforts to Increase the Identification of the Number of Gifted Minority Children.

    ERIC Educational Resources Information Center

    Davis, Paul I.

    The document reports on a study of community-based identification of gifted and talented Black middle-school students, as an adjunct to formal identification procedures. A developmental framework for the identification of the gifted minority child was distributed to 17 known leaders in the Black community (including ministers, youth leaders in…

  9. The Identification of Threshold Concepts: A Review of Theoretical Complexities and Methodological Challenges

    ERIC Educational Resources Information Center

    Barradell, Sarah

    2013-01-01

    While the study of threshold concepts is a growing area of research, their identification has not proven to be an easy process. However, identification matters because of the potential impact of threshold concepts on the learning experiences of students. A dialogue amongst lecturers and/or students is common to the literature on identification of…

  10. Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast.

    PubMed

    Dhiman, Neelam; Hall, Leslie; Wohlfiel, Sherri L; Buckwalter, Seanne P; Wengenack, Nancy L

    2011-04-01

    Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry was compared to phenotypic testing for yeast identification. MALDI-TOF mass spectrometry yielded 96.3% and 84.5% accurate species level identifications (spectral scores, ≥ 1.8) for 138 common and 103 archived strains of yeast. MALDI-TOF mass spectrometry is accurate, rapid (5.1 min of hands-on time/identification), and cost-effective ($0.50/sample) for yeast identification in the clinical laboratory.

  11. Encrypted data stream identification using randomness sparse representation and fuzzy Gaussian mixture model

    NASA Astrophysics Data System (ADS)

    Zhang, Hong; Hou, Rui; Yi, Lei; Meng, Juan; Pan, Zhisong; Zhou, Yuhuan

    2016-07-01

    The accurate identification of encrypted data stream helps to regulate illegal data, detect network attacks and protect users' information. In this paper, a novel encrypted data stream identification algorithm is introduced. The proposed method is based on randomness characteristics of encrypted data stream. We use a l1-norm regularized logistic regression to improve sparse representation of randomness features and Fuzzy Gaussian Mixture Model (FGMM) to improve identification accuracy. Experimental results demonstrate that the method can be adopted as an effective technique for encrypted data stream identification.

  12. The Impact of Early Design Phase Risk Identification Biases on Space System Project Performance

    NASA Technical Reports Server (NTRS)

    Reeves, John D., Jr.; Eveleigh, Tim; Holzer, Thomas; Sarkani, Shahryar

    2012-01-01

    Risk identification during the early design phases of complex systems is commonly implemented but often fails to result in the identification of events and circumstances that truly challenge project performance. Inefficiencies in cost and schedule estimation are usually held accountable for cost and schedule overruns, but the true root cause is often the realization of programmatic risks. A deeper understanding of frequent risk identification trends and biases pervasive during space system design and development is needed, for it would lead to improved execution of existing identification processes and methods.

  13. Anti-collision radio-frequency identification system using passive SAW tags

    NASA Astrophysics Data System (ADS)

    Sorokin, A. V.; Shepeta, A. P.

    2017-06-01

    Modern multi sensor systems should have high operating speed and resistance to climate impacts. Radiofrequency systems use passive SAW tags for identification items and vehicles. These tags find application in industry, traffic remote control systems, and railway remote traffic control systems for identification and speed measuring. However, collision of the passive SAW RFID tags hinders development passive RFID SAW technology in Industry. The collision problem for passive SAW tags leads for incorrect identification and encoding each tag. In our researching, we suggest approach for identification of several passive SAW tags in collision case.

  14. Sparse Matrix for ECG Identification with Two-Lead Features.

    PubMed

    Tseng, Kuo-Kun; Luo, Jiao; Hegarty, Robert; Wang, Wenmin; Haiting, Dong

    2015-01-01

    Electrocardiograph (ECG) human identification has the potential to improve biometric security. However, improvements in ECG identification and feature extraction are required. Previous work has focused on single lead ECG signals. Our work proposes a new algorithm for human identification by mapping two-lead ECG signals onto a two-dimensional matrix then employing a sparse matrix method to process the matrix. And that is the first application of sparse matrix techniques for ECG identification. Moreover, the results of our experiments demonstrate the benefits of our approach over existing methods.

  15. Variation in Microbial Identification System Accuracy for Yeast Identification Depending on Commercial Source of Sabouraud Dextrose Agar

    PubMed Central

    Kellogg, James A.; Bankert, David A.; Chaturvedi, Vishnu

    1999-01-01

    The accuracy of the Microbial Identification System (MIS; MIDI, Inc.) for identification of yeasts to the species level was compared by using 438 isolates grown on prepoured BBL Sabouraud dextrose agar (SDA) and prepoured Remel SDA. Correct identification was observed for 326 (74%) of the yeasts cultured on BBL SDA versus only 214 (49%) of yeasts grown on Remel SDA (P < 0.001). The commercial source of the SDA used in the MIS procedure significantly influences the system’s accuracy. PMID:10325387

  16. MALDI-TOF-mass spectrometry applications in clinical microbiology.

    PubMed

    Seng, Piseth; Rolain, Jean-Marc; Fournier, Pierre Edouard; La Scola, Bernard; Drancourt, Michel; Raoult, Didier

    2010-11-01

    MALDI-TOF-mass spectrometry (MS) has been successfully adapted for the routine identification of microorganisms in clinical microbiology laboratories in the past 10 years. This revolutionary technique allows for easier and faster diagnosis of human pathogens than conventional phenotypic and molecular identification methods, with unquestionable reliability and cost-effectiveness. This article will review the application of MALDI-TOF-MS tools in routine clinical diagnosis, including the identification of bacteria at the species, subspecies, strain and lineage levels, and the identification of bacterial toxins and antibiotic-resistance type. We will also discuss the application of MALDI-TOF-MS tools in the identification of Archaea, eukaryotes and viruses. Pathogenic identification from colony-cultured, blood-cultured, urine and environmental samples is also reviewed.

  17. Hierarchical minutiae matching for fingerprint and palmprint identification.

    PubMed

    Chen, Fanglin; Huang, Xiaolin; Zhou, Jie

    2013-12-01

    Fingerprints and palmprints are the most common authentic biometrics for personal identification, especially for forensic security. Previous research have been proposed to speed up the searching process in fingerprint and palmprint identification systems, such as those based on classification or indexing, in which the deterioration of identification accuracy is hard to avert. In this paper, a novel hierarchical minutiae matching algorithm for fingerprint and palmprint identification systems is proposed. This method decomposes the matching step into several stages and rejects many false fingerprints or palmprints on different stages, thus it can save much time while preserving a high identification rate. Experimental results show that the proposed algorithm can save almost 50% searching time compared with traditional methods and illustrate its effectiveness.

  18. Identification accuracy of children versus adults: a meta-analysis.

    PubMed

    Pozzulo, J D; Lindsay, R C

    1998-10-01

    Identification accuracy of children and adults was examined in a meta-analysis. Preschoolers (M = 4 years) were less likely than adults to make correct identifications. Children over the age of 5 did not differ significantly from adults with regard to correct identification rate. Children of all ages examined were less likely than adults to correctly reject a target-absent lineup. Even adolescents (M = 12-13 years) did not reach an adult rate of correct rejection. Compared to simultaneous lineup presentation, sequential lineups increased the child-adult gap for correct rejections. Providing child witnesses with identification practice or training did not increase their correct rejection rates. Suggestions for children's inability to correctly reject target-absent lineups are discussed. Future directions for identification research are presented.

  19. Eyewitness identification accuracy and response latency: the unruly 10-12-second rule.

    PubMed

    Weber, Nathan; Brewer, Neil; Wells, Gary L; Semmler, Carolyn; Keast, Amber

    2004-09-01

    Data are reported from 3,213 research eyewitnesses confirming that accurate eyewitness identifications from lineups are made faster than are inaccurate identifications. However, consistent with predictions from the recognition and search literatures, the authors did not find support for the "10-12-s rule" in which lineup identifications faster than 10-12 s maximally discriminate between accurate and inaccurate identifications (D. Dunning & S. Perretta, 2002). Instead, the time frame that proved most discriminating was highly variable across experiments, ranging from 5 s to 29 s, and the maximally discriminating time was often unimpressive in its ability to sort accurate from inaccurate identifications. The authors suggest several factors that are likely to moderate the 10-12-s rule. (c) 2004 APA, all rights reserved.

  20. Searching for the elusive gift: advances in talent identification in sport.

    PubMed

    Mann, David L; Dehghansai, Nima; Baker, Joseph

    2017-08-01

    The incentives for sport organizations to identify talented athletes from a young age continue to grow, yet effective talent identification remains a challenging task. This opinion paper examines recent advances in talent identification, focusing in particular on the emergence of new approaches that may offer promise to identify talent (e.g., small-sided games, genetic testing, and advanced statistical analyses). We appraise new multi-disciplinary and large-scale population studies of talent identification, provide a consideration of the most recent psychological predictors of performance, examine the emergence of new approaches that strive to diminish biases in talent identification, and look at the rise in interest in talent identification in Paralympic sport. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  1. [Hydrophidae identification through analysis on Cyt b gene barcode].

    PubMed

    Liao, Li-xi; Zeng, Ke-wu; Tu, Peng-fei

    2015-08-01

    Hydrophidae, one of the precious traditional Chinese medicines, is generally drily preserved to prevent corruption, but it is hard to identify the species of Hydrophidae through the appearance because of the change due to the drying process. The identification through analysis on gene barcode, a new technique in species identification, can avoid the problem. The gene barcodes of the 6 species of Hydrophidae like Lapemis hardwickii were aquired through DNA extraction and gene sequencing. These barcodes were then in sequence alignment and test the identification efficency by BLAST. Our results revealed that the barcode sequences performed high identification efficiency, and had obvious difference between intra- and inter-species. These all indicated that Cyt b DNA barcoding can confirm the Hydrophidae identification.

  2. On the problem of modeling for parameter identification in distributed structures

    NASA Technical Reports Server (NTRS)

    Norris, Mark A.; Meirovitch, Leonard

    1988-01-01

    Structures are often characterized by parameters, such as mass and stiffness, that are spatially distributed. Parameter identification of distributed structures is subject to many of the difficulties involved in the modeling problem, and the choice of the model can greatly affect the results of the parameter identification process. Analogously to control spillover in the control of distributed-parameter systems, identification spillover is shown to exist as well and its effect is to degrade the parameter estimates. Moreover, as in modeling by the Rayleigh-Ritz method, it is shown that, for a Rayleigh-Ritz type identification algorithm, an inclusion principle exists in the identification of distributed-parameter systems as well, so that the identified natural frequencies approach the actual natural frequencies monotonically from above.

  3. [Isolation and identification methods of enterobacteria group and its technological advancement].

    PubMed

    Furuta, Itaru

    2007-08-01

    In the last half-century, isolation and identification methods of enterobacteria groups have markedly improved by technological advancement. Clinical microbiology tests have changed overtime from tube methods to commercial identification kits and automated identification. Tube methods are the original method for the identification of enterobacteria groups, that is, a basically essential method to recognize bacterial fermentation and biochemical principles. In this paper, traditional tube tests are discussed, such as the utilization of carbohydrates, indole, methyl red, and citrate and urease tests. Commercial identification kits and automated instruments by computer based analysis as current methods are also discussed, and those methods provide rapidity and accuracy. Nonculture techniques of nucleic acid typing methods using PCR analysis, and immunochemical methods using monoclonal antibodies can be further developed.

  4. Visuo-spatial interference affects the identification of emotional facial expressions in unmedicated Parkinson's patients.

    PubMed

    García-Rodríguez, Beatriz; Guillén, Carmen Casares; Barba, Rosa Jurado; io Valladolid, Gabriel Rub; Arjona, José Antonio Molina; Ellgring, Heiner

    2012-02-15

    There is evidence that visuo-spatial capacity can become overloaded when processing a secondary visual task (Dual Task, DT), as occurs in daily life. Hence, we investigated the influence of the visuo-spatial interference in the identification of emotional facial expressions (EFEs) in early stages of Parkinson's disease (PD). We compared the identification of 24 emotional faces that illustrate six basic emotions in, unmedicated recently diagnosed PD patients (16) and healthy adults (20), under two different conditions: a) simple EFE identification, and b) identification with a concurrent visuo-spatial task (Corsi Blocks). EFE identification by PD patients was significantly worse than that of healthy adults when combined with another visual stimulus. Published by Elsevier B.V.

  5. Multicenter Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Gram-Positive Aerobic Bacteria

    PubMed Central

    Burnham, Carey-Ann D.; Bythrow, Maureen; Garner, Omai B.; Ginocchio, Christine C.; Jennemann, Rebecca; Lewinski, Michael A.; Manji, Ryhana; Mochon, A. Brian; Procop, Gary W.; Richter, Sandra S.; Sercia, Linda; Westblade, Lars F.; Ferraro, Mary Jane; Branda, John A.

    2013-01-01

    Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF) is gaining momentum as a tool for bacterial identification in the clinical microbiology laboratory. Compared with conventional methods, this technology can more readily and conveniently identify a wide range of organisms. Here, we report the findings from a multicenter study to evaluate the Vitek MS v2.0 system (bioMérieux, Inc.) for the identification of aerobic Gram-positive bacteria. A total of 1,146 unique isolates, representing 13 genera and 42 species, were analyzed, and results were compared to those obtained by nucleic acid sequence-based identification as the reference method. For 1,063 of 1,146 isolates (92.8%), the Vitek MS provided a single identification that was accurate to the species level. For an additional 31 isolates (2.7%), multiple possible identifications were provided, all correct at the genus level. Mixed-genus or single-choice incorrect identifications were provided for 18 isolates (1.6%). Although no identification was obtained for 33 isolates (2.9%), there was no specific bacterial species for which the Vitek MS consistently failed to provide identification. In a subset of 463 isolates representing commonly encountered important pathogens, 95% were accurately identified to the species level and there were no misidentifications. Also, in all but one instance, the Vitek MS correctly differentiated Streptococcus pneumoniae from other viridans group streptococci. The findings demonstrate that the Vitek MS system is highly accurate for the identification of Gram-positive aerobic bacteria in the clinical laboratory setting. PMID:23658261

  6. Direct identification of pathogens from positive blood cultures using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry.

    PubMed

    Rodríguez-Sánchez, B; Sánchez-Carrillo, C; Ruiz, A; Marín, M; Cercenado, E; Rodríguez-Créixems, M; Bouza, E

    2014-07-01

    In recent years, matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has proved a rapid and reliable method for the identification of bacteria and yeasts that have already been isolated. The objective of this study was to evaluate this technology as a routine method for the identification of microorganisms directly from blood culture bottles (BCBs), before isolation, in a large collection of samples. For this purpose, 1000 positive BCBs containing 1085 microorganisms have been analysed by conventional phenotypic methods and by MALDI-TOF MS. Discrepancies have been resolved using molecular methods: the amplification and sequencing of the 16S rRNA gene or the Superoxide Dismutase gene (sodA) for streptococcal isolates. MALDI-TOF predicted a species- or genus-level identification of 81.4% of the analysed microorganisms. The analysis by episode yielded a complete identification of 814 out of 1000 analysed episodes (81.4%). MALDI-TOF identification is available for clinicians within hours of a working shift, as oppose to 18 h later when conventional identification methods are performed. Moreover, although further improvement of sample preparation for polymicrobial BCBs is required, the identification of more than one pathogen in the same BCB provides a valuable indication of unexpected pathogens when their presence may remain undetected in Gram staining. Implementation of MALDI-TOF identification directly from the BCB provides a rapid and reliable identification of the causal pathogen within hours. © 2013 The Authors Clinical Microbiology and Infection © 2013 European Society of Clinical Microbiology and Infectious Diseases.

  7. Effects of solid-medium type on routine identification of bacterial isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.

    PubMed

    Anderson, Neil W; Buchan, Blake W; Riebe, Katherine M; Parsons, Lauren N; Gnacinski, Stacy; Ledeboer, Nathan A

    2012-03-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a rapid method for the identification of bacteria. Factors that may alter protein profiles, including growth conditions and presence of exogenous substances, could hinder identification. Bacterial isolates identified by conventional methods were grown on various media and identified using the MALDI Biotyper (Bruker Daltonics, Billerica, MA) using a direct smear method and an acid extraction method. Specimens included 23 Pseudomonas isolates grown on blood agar, Pseudocel (CET), and MacConkey agar (MAC); 20 Staphylococcus isolates grown on blood agar, colistin-nalidixic acid agar (CNA), and mannitol salt agar (MSA); and 25 enteric isolates grown on blood agar, xylose lysine deoxycholate agar (XLD), Hektoen enteric agar (HE), salmonella-shigella agar (SS), and MAC. For Pseudomonas spp., the identification rate to genus using the direct method was 83% from blood, 78% from MAC, and 94% from CET. For Staphylococcus isolates, the identification rate to genus using the direct method was 95% from blood, 75% from CNA, and 95% from MSA. For enteric isolates, the identification rate to genus using the direct method was 100% from blood, 100% from MAC, 100% from XLD, 92% from HE, and 87% from SS. Extraction enhanced identification rates. The direct method of MALDI-TOF analysis of bacteria from selective and differential media yields identifications of varied confidence. Notably, Staphylococci spp. from CNA exhibit low identification rates. Extraction enhances identification rates and is recommended for colonies from this medium.

  8. 3D topography measurements on correlation cells—a new approach to forensic ballistics identifications

    NASA Astrophysics Data System (ADS)

    Song, John; Chu, Wei; Tong, Mingsi; Soons, Johannes

    2014-06-01

    Based on three-dimensional (3D) topography measurements on correlation cells, the National Institute of Standards and Technology (NIST) has developed the ‘NIST Ballistics Identification System (NBIS)’ aimed at accurate ballistics identifications and fast ballistics evidence searches. The 3D topographies are divided into arrays of correlation cells to identify ‘valid correlation areas’ and eliminate ‘invalid correlation areas’ from the matching and identification procedure. A ‘congruent matching cells’ (CMC)’ method using three types of identification parameters of the paired correlation cells (cross correlation function maximum CCFmax, spatial registration position in x-y and registration angle θ) is used for high accuracy ballistics identifications. ‘Synchronous processing’ is proposed for correlating multiple cell pairs at the same time to increase the correlation speed. The proposed NBIS can be used for correlations of both geometrical topographies and optical intensity images. All the correlation parameters and algorithms are in the public domain and subject to open tests. An error rate reporting procedure has been developed that can greatly add to the scientific support for the firearm and toolmark identification specialty, and give confidence to the trier of fact in court proceedings. The NBIS is engineered to employ transparent identification parameters and criteria, statistical models and correlation algorithms. In this way, interoperability between different ballistics identification systems can be more easily achieved. This interoperability will make the NBIS suitable for ballistics identifications and evidence searches with large national databases, such as the National Integrated Ballistic Information Network in the United States.

  9. Reduction in Hospital-Wide Clinical Laboratory Specimen Identification Errors following Process Interventions: A 10-Year Retrospective Observational Study

    PubMed Central

    Ning, Hsiao-Chen; Lin, Chia-Ni; Chiu, Daniel Tsun-Yee; Chang, Yung-Ta; Wen, Chiao-Ni; Peng, Shu-Yu; Chu, Tsung-Lan; Yu, Hsin-Ming; Wu, Tsu-Lan

    2016-01-01

    Background Accurate patient identification and specimen labeling at the time of collection are crucial steps in the prevention of medical errors, thereby improving patient safety. Methods All patient specimen identification errors that occurred in the outpatient department (OPD), emergency department (ED), and inpatient department (IPD) of a 3,800-bed academic medical center in Taiwan were documented and analyzed retrospectively from 2005 to 2014. To reduce such errors, the following series of strategies were implemented: a restrictive specimen acceptance policy for the ED and IPD in 2006; a computer-assisted barcode positive patient identification system for the ED and IPD in 2007 and 2010, and automated sample labeling combined with electronic identification systems introduced to the OPD in 2009. Results Of the 2000345 specimens collected in 2005, 1023 (0.0511%) were identified as having patient identification errors, compared with 58 errors (0.0015%) among 3761238 specimens collected in 2014, after serial interventions; this represents a 97% relative reduction. The total number (rate) of institutional identification errors contributed from the ED, IPD, and OPD over a 10-year period were 423 (0.1058%), 556 (0.0587%), and 44 (0.0067%) errors before the interventions, and 3 (0.0007%), 52 (0.0045%) and 3 (0.0001%) after interventions, representing relative 99%, 92% and 98% reductions, respectively. Conclusions Accurate patient identification is a challenge of patient safety in different health settings. The data collected in our study indicate that a restrictive specimen acceptance policy, computer-generated positive identification systems, and interdisciplinary cooperation can significantly reduce patient identification errors. PMID:27494020

  10. Multicenter evaluation of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of Gram-positive aerobic bacteria.

    PubMed

    Rychert, Jenna; Burnham, Carey-Ann D; Bythrow, Maureen; Garner, Omai B; Ginocchio, Christine C; Jennemann, Rebecca; Lewinski, Michael A; Manji, Ryhana; Mochon, A Brian; Procop, Gary W; Richter, Sandra S; Sercia, Linda; Westblade, Lars F; Ferraro, Mary Jane; Branda, John A

    2013-07-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) is gaining momentum as a tool for bacterial identification in the clinical microbiology laboratory. Compared with conventional methods, this technology can more readily and conveniently identify a wide range of organisms. Here, we report the findings from a multicenter study to evaluate the Vitek MS v2.0 system (bioMérieux, Inc.) for the identification of aerobic Gram-positive bacteria. A total of 1,146 unique isolates, representing 13 genera and 42 species, were analyzed, and results were compared to those obtained by nucleic acid sequence-based identification as the reference method. For 1,063 of 1,146 isolates (92.8%), the Vitek MS provided a single identification that was accurate to the species level. For an additional 31 isolates (2.7%), multiple possible identifications were provided, all correct at the genus level. Mixed-genus or single-choice incorrect identifications were provided for 18 isolates (1.6%). Although no identification was obtained for 33 isolates (2.9%), there was no specific bacterial species for which the Vitek MS consistently failed to provide identification. In a subset of 463 isolates representing commonly encountered important pathogens, 95% were accurately identified to the species level and there were no misidentifications. Also, in all but one instance, the Vitek MS correctly differentiated Streptococcus pneumoniae from other viridans group streptococci. The findings demonstrate that the Vitek MS system is highly accurate for the identification of Gram-positive aerobic bacteria in the clinical laboratory setting.

  11. BoB, a best-of-breed automated text de-identification system for VHA clinical documents.

    PubMed

    Ferrández, Oscar; South, Brett R; Shen, Shuying; Friedlin, F Jeffrey; Samore, Matthew H; Meystre, Stéphane M

    2013-01-01

    De-identification allows faster and more collaborative clinical research while protecting patient confidentiality. Clinical narrative de-identification is a tedious process that can be alleviated by automated natural language processing methods. The goal of this research is the development of an automated text de-identification system for Veterans Health Administration (VHA) clinical documents. We devised a novel stepwise hybrid approach designed to improve the current strategies used for text de-identification. The proposed system is based on a previous study on the best de-identification methods for VHA documents. This best-of-breed automated clinical text de-identification system (aka BoB) tackles the problem as two separate tasks: (1) maximize patient confidentiality by redacting as much protected health information (PHI) as possible; and (2) leave de-identified documents in a usable state preserving as much clinical information as possible. We evaluated BoB with a manually annotated corpus of a variety of VHA clinical notes, as well as with the 2006 i2b2 de-identification challenge corpus. We present evaluations at the instance- and token-level, with detailed results for BoB's main components. Moreover, an existing text de-identification system was also included in our evaluation. BoB's design efficiently takes advantage of the methods implemented in its pipeline, resulting in high sensitivity values (especially for sensitive PHI categories) and a limited number of false positives. Our system successfully addressed VHA clinical document de-identification, and its hybrid stepwise design demonstrates robustness and efficiency, prioritizing patient confidentiality while leaving most clinical information intact.

  12. Does ethical membership matter? Moral identification and its organizational implications.

    PubMed

    May, Douglas R; Chang, Young K; Shao, Ruodan

    2015-05-01

    This research meaningfully connects the literatures on identification and business ethics by proposing the new construct of moral identification. Moral identification is defined here as the perception of oneness or belongingness associated with an organization that exhibits ethical traits (e.g., care, kindness, and compassion), which also involves a deliberate concern of the membership with an ethical organization. Integrating social identity theory with theory on the moral self, this research examines an overall theoretical model where moral identification plays a significant role in explaining employee attraction, motivation, and retention (i.e., 3 components of the overall theoretical framework). These components were examined separately in 3 empirical studies and findings from these studies first revealed that moral identification explained why job seekers with strong (vs. weak) moral identities were more attracted to a socially responsible organization (Study 1). Second, moral identification was associated with lower employee unethical proorganizational behavior (Study 2). Finally, moral identification was negatively related to employees' turnover intentions. Organizations' legal compliance moderated this relation such that it was stronger when organizations have higher (vs. lower) levels of legal compliance (Study 3). Taken together, these studies suggest that moral identification offers new insights in explaining both potential and current employees' behaviors when morality is contextually relevant and subjectively meaningful. Theoretical contributions and practical implications are discussed. (c) 2015 APA, all rights reserved.

  13. Reliability and validity of a talent identification test battery for seated and standing Paralympic throws.

    PubMed

    Spathis, Jemima Grace; Connick, Mark James; Beckman, Emma Maree; Newcombe, Peter Anthony; Tweedy, Sean Michael

    2015-01-01

    Paralympic throwing events for athletes with physical impairments comprise seated and standing javelin, shot put, discus and seated club throwing. Identification of talented throwers would enable prediction of future success and promote participation; however, a valid and reliable talent identification battery for Paralympic throwing has not been reported. This study evaluates the reliability and validity of a talent identification battery for Paralympic throws. Participants were non-disabled so that impairment would not confound analyses, and results would provide an indication of normative performance. Twenty-eight non-disabled participants (13 M; 15 F) aged 23.6 years (±5.44) performed five kinematically distinct criterion throws (three seated, two standing) and nine talent identification tests (three anthropometric, six motor); 23 were tested a second time to evaluate test-retest reliability. Talent identification test-retest reliability was evaluated using Intra-class Correlation Coefficient (ICC) and Bland-Altman plots (Limits of Agreement). Spearman's correlation assessed strength of association between criterion throws and talent identification tests. Reliability was generally acceptable (mean ICC = 0.89), but two seated talent identification tests require more extensive familiarisation. Correlation strength (mean rs = 0.76) indicated that the talent identification tests can be used to validly identify individuals with competitively advantageous attributes for each of the five kinematically distinct throwing activities. Results facilitate further research in this understudied area.

  14. Evaluation of the Microbial Identification System for identification of clinically isolated yeasts.

    PubMed Central

    Crist, A E; Johnson, L M; Burke, P J

    1996-01-01

    The Microbial Identification System (MIS; Microbial ID, Inc., Newark, Del.) was evaluated for the identification of 550 clinically isolated yeasts. The organisms evaluated were fresh clinical isolates identified by methods routinely used in our laboratory (API 20C and conventional methods) and included Candida albicans (n = 294), C. glabrata (n = 145), C. tropicalis (n = 58), C. parapsilosis (n = 33), and other yeasts (n = 20). In preparation for fatty acid analysis, yeasts were inoculated onto Sabouraud dextrose agar and incubated at 28 degrees C for 24 h. Yeasts were harvested, saponified, derivatized, and extracted, and fatty acid analysis was performed according to the manufacturer's instructions. Fatty acid profiles were analyzed, and computer identifications were made with the Yeast Clinical Library (database version 3.8). Of the 550 isolates tested, 374 (68.0%) were correctly identified to the species level, with 87 (15.8%) being incorrectly identified and 89 (16.2%) giving no identification. Repeat testing of isolates giving no identification resulted in an additional 18 isolates being correctly identified. This gave the MIS an overall identification rate of 71.3%. The most frequently misidentified yeast was C. glabrata, which was identified as Saccharomyces cerevisiae 32.4% of the time. On the basis of these results, the MIS, with its current database, does not appear suitable for the routine identification of clinically important yeasts. PMID:8880489

  15. Integrated identification and control for nanosatellites reclaiming failed satellite

    NASA Astrophysics Data System (ADS)

    Han, Nan; Luo, Jianjun; Ma, Weihua; Yuan, Jianping

    2018-05-01

    Using nanosatellites to reclaim a failed satellite needs nanosatellites to attach to its surface to take over its attitude control function. This is challenging, since parameters including the inertia matrix of the combined spacecraft and the relative attitude information of attached nanosatellites with respect to the given body-fixed frame of the failed satellite are all unknown after the attachment. Besides, if the total control capacity needs to be increased during the reclaiming process by new nanosatellites, real-time parameters updating will be necessary. For these reasons, an integrated identification and control method is proposed in this paper, which enables the real-time parameters identification and attitude takeover control to be conducted concurrently. Identification of the inertia matrix of the combined spacecraft and the relative attitude information of attached nanosatellites are both considered. To guarantee sufficient excitation for the identification of the inertia matrix, a modified identification equation is established by filtering out sample points leading to ill-conditioned identification, and the identification performance of the inertia matrix is improved. Based on the real-time estimated inertia matrix, an attitude takeover controller is designed, the stability of the controller is analysed using Lyapunov method. The commanded control torques are allocated to each nanosatellite while the control saturation constraint being satisfied using the Quadratic Programming (QP) method. Numerical simulations are carried out to demonstrate the feasibility and effectiveness of the proposed integrated identification and control method.

  16. 25 CFR 115.410 - What types of identification will the BIA or OTFM accept as “verifiable photo identification”?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... as âverifiable photo identificationâ? 115.410 Section 115.410 Indians BUREAU OF INDIAN AFFAIRS...: Minors § 115.410 What types of identification will the BIA or OTFM accept as “verifiable photo identification”? BIA or OTFM will accept the following forms of identification as “verifiable photo...

  17. 25 CFR 115.410 - What types of identification will the BIA or OTFM accept as “verifiable photo identification”?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... as âverifiable photo identificationâ? 115.410 Section 115.410 Indians BUREAU OF INDIAN AFFAIRS...: Minors § 115.410 What types of identification will the BIA or OTFM accept as “verifiable photo identification”? BIA or OTFM will accept the following forms of identification as “verifiable photo...

  18. 25 CFR 115.410 - What types of identification will the BIA or OTFM accept as “verifiable photo identification”?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... as âverifiable photo identificationâ? 115.410 Section 115.410 Indians BUREAU OF INDIAN AFFAIRS...: Minors § 115.410 What types of identification will the BIA or OTFM accept as “verifiable photo identification”? BIA or OTFM will accept the following forms of identification as “verifiable photo...

  19. 25 CFR 115.410 - What types of identification will the BIA or OTFM accept as “verifiable photo identification”?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... as âverifiable photo identificationâ? 115.410 Section 115.410 Indians BUREAU OF INDIAN AFFAIRS...: Minors § 115.410 What types of identification will the BIA or OTFM accept as “verifiable photo identification”? BIA or OTFM will accept the following forms of identification as “verifiable photo...

  20. 25 CFR 115.410 - What types of identification will the BIA or OTFM accept as “verifiable photo identification”?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... as âverifiable photo identificationâ? 115.410 Section 115.410 Indians BUREAU OF INDIAN AFFAIRS...: Minors § 115.410 What types of identification will the BIA or OTFM accept as “verifiable photo identification”? BIA or OTFM will accept the following forms of identification as “verifiable photo...

  1. 41 CFR 102-34.185 - What license plates do we use on motor vehicles that are exempt from motor vehicle identification...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... we use on motor vehicles that are exempt from motor vehicle identification requirements? 102-34.185... Registering Motor Vehicles Identification Exemptions § 102-34.185 What license plates do we use on motor vehicles that are exempt from motor vehicle identification requirements? For motor vehicles that are exempt...

  2. 41 CFR 102-34.185 - What license plates do we use on motor vehicles that are exempt from motor vehicle identification...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... we use on motor vehicles that are exempt from motor vehicle identification requirements? 102-34.185... Registering Motor Vehicles Identification Exemptions § 102-34.185 What license plates do we use on motor vehicles that are exempt from motor vehicle identification requirements? For motor vehicles that are exempt...

  3. 41 CFR 102-34.185 - What license plates do we use on motor vehicles that are exempt from motor vehicle identification...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... we use on motor vehicles that are exempt from motor vehicle identification requirements? 102-34.185... Registering Motor Vehicles Identification Exemptions § 102-34.185 What license plates do we use on motor vehicles that are exempt from motor vehicle identification requirements? For motor vehicles that are exempt...

  4. 41 CFR 102-34.185 - What license plates do we use on motor vehicles that are exempt from motor vehicle identification...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... we use on motor vehicles that are exempt from motor vehicle identification requirements? 102-34.185... Registering Motor Vehicles Identification Exemptions § 102-34.185 What license plates do we use on motor vehicles that are exempt from motor vehicle identification requirements? For motor vehicles that are exempt...

  5. 41 CFR 102-34.185 - What license plates do we use on motor vehicles that are exempt from motor vehicle identification...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... we use on motor vehicles that are exempt from motor vehicle identification requirements? 102-34.185... Registering Motor Vehicles Identification Exemptions § 102-34.185 What license plates do we use on motor vehicles that are exempt from motor vehicle identification requirements? For motor vehicles that are exempt...

  6. Contribution of low-frequency harmonics to Mandarin Chinese tone identification in quiet and six-talker babble background.

    PubMed

    Liu, Chang; Azimi, Behnam; Bhandary, Moulesh; Hu, Yi

    2014-01-01

    The goal of this study was to investigate Mandarin Chinese tone identification in quiet and multi-talker babble conditions for normal-hearing listeners. Tone identification was measured with speech stimuli and stimuli with low and/or high harmonics that were embedded in three Mandarin vowels with two fundamental frequencies. There were six types of stimuli: all harmonics (All), low harmonics (Low), high harmonics (High), and the first (H1), second (H2), and third (H3) harmonic. Results showed that, for quiet conditions, individual harmonics carried frequency contour information well enough for tone identification with high accuracy; however, in noisy conditions, tone identification with individual low harmonics (e.g., H1, H2, and H3) was significantly lower than that with the Low, High, and All harmonics. Moreover, tone identification with individual harmonics in noise was lower for a low F0 than for a high F0, and was also dependent on vowel category. Tone identification with individual low-frequency harmonics was accounted for by local signal-to-noise ratios, indicating that audibility of harmonics in noise may play a primary role in tone identification.

  7. Discrimination of Chinese Sauce liquor using FT-IR and two-dimensional correlation IR spectroscopy

    NASA Astrophysics Data System (ADS)

    Sun, Su-Qin; Li, Chang-Wen; Wei, Ji-Ping; Zhou, Qun; Noda, Isao

    2006-11-01

    We applied the three-step IR macro-fingerprint identification method to obtain the IR characteristic fingerprints of so-called Chinese Sauce liquor (Moutai liquor and Kinsly liquor) and a counterfeit Moutai. These fingerprints can be used for the identification and discrimination of similar liquor products. The comparison of their conventional IR spectra, as the first step of identification, shows that the primary difference in Sauce liquor is the intensity of characteristic peaks at 1592 and 1225 cm -1. The comparison of the second derivative IR spectra, as the second step of identification, shows that the characteristic absorption in 1400-1800 cm -1 is substantially different. The comparison of 2D-IR correlation spectra, as the third and final step of identification, can discriminate the liquors from another direction. Furthermore, the method was successfully applied to the discrimination of a counterfeit Moutai from the genuine Sauce liquor. The success of the three-step IR macro-fingerprint identification to provide a rapid and effective method for the identification of Chinese liquor suggests the potential extension of this technique to the identification and discrimination of other wine and spirits, as well.

  8. Forensic bitemark identification: weak foundations, exaggerated claims.

    PubMed

    Saks, Michael J; Albright, Thomas; Bohan, Thomas L; Bierer, Barbara E; Bowers, C Michael; Bush, Mary A; Bush, Peter J; Casadevall, Arturo; Cole, Simon A; Denton, M Bonner; Diamond, Shari Seidman; Dioso-Villa, Rachel; Epstein, Jules; Faigman, David; Faigman, Lisa; Fienberg, Stephen E; Garrett, Brandon L; Giannelli, Paul C; Greely, Henry T; Imwinkelried, Edward; Jamieson, Allan; Kafadar, Karen; Kassirer, Jerome P; Koehler, Jonathan 'Jay'; Korn, David; Mnookin, Jennifer; Morrison, Alan B; Murphy, Erin; Peerwani, Nizam; Peterson, Joseph L; Risinger, D Michael; Sensabaugh, George F; Spiegelman, Clifford; Stern, Hal; Thompson, William C; Wayman, James L; Zabell, Sandy; Zumwalt, Ross E

    2016-12-01

    Several forensic sciences, especially of the pattern-matching kind, are increasingly seen to lack the scientific foundation needed to justify continuing admission as trial evidence. Indeed, several have been abolished in the recent past. A likely next candidate for elimination is bitemark identification. A number of DNA exonerations have occurred in recent years for individuals convicted based on erroneous bitemark identifications. Intense scientific and legal scrutiny has resulted. An important National Academies review found little scientific support for the field. The Texas Forensic Science Commission recently recommended a moratorium on the admission of bitemark expert testimony. The California Supreme Court has a case before it that could start a national dismantling of forensic odontology. This article describes the (legal) basis for the rise of bitemark identification and the (scientific) basis for its impending fall. The article explains the general logic of forensic identification, the claims of bitemark identification, and reviews relevant empirical research on bitemark identification-highlighting both the lack of research and the lack of support provided by what research does exist. The rise and possible fall of bitemark identification evidence has broader implications-highlighting the weak scientific culture of forensic science and the law's difficulty in evaluating and responding to unreliable and unscientific evidence.

  9. Antecedents and consequences of customer-company identification: expanding the role of relationship marketing.

    PubMed

    Ahearne, Michael; Bhattacharya, C B; Gruen, Thomas

    2005-05-01

    This article presents an empirical test of organizational identification in the context of customer-company (C-C) relationships. It investigates whether customers identify with companies and what the antecedents and consequences of such identification are. The model posits that perceived company characteristics, construed external image, and the perception of the company's boundary-spanning agent lead to C-C identification. In turn, such identification is expected to impact both in-role behavior (i.e., product utilization) as well as extra-role behavior (i.e., citizenship). The model was tested in a consultative selling context of pharmaceutical sales reps calling on physicians. Results from the empirical test indicated that customers do indeed identify with organizations and that C-C identification positively impacts both product utilization behavior and extra-role behavior even when the effect of brand perception is accounted for. Second, the study found that the organization's characteristics as well as the salesperson's characteristics contributed to the development of C-C identification.

  10. How automated image analysis techniques help scientists in species identification and classification?

    PubMed

    Yousef Kalafi, Elham; Town, Christopher; Kaur Dhillon, Sarinder

    2017-09-04

    Identification of taxonomy at a specific level is time consuming and reliant upon expert ecologists. Hence the demand for automated species identification increased over the last two decades. Automation of data classification is primarily focussed on images, incorporating and analysing image data has recently become easier due to developments in computational technology. Research efforts in identification of species include specimens' image processing, extraction of identical features, followed by classifying them into correct categories. In this paper, we discuss recent automated species identification systems, categorizing and evaluating their methods. We reviewed and compared different methods in step by step scheme of automated identification and classification systems of species images. The selection of methods is influenced by many variables such as level of classification, number of training data and complexity of images. The aim of writing this paper is to provide researchers and scientists an extensive background study on work related to automated species identification, focusing on pattern recognition techniques in building such systems for biodiversity studies.

  11. What Is New in Clinical Microbiology—Microbial Identification by MALDI-TOF Mass Spectrometry

    PubMed Central

    Murray, Patrick R.

    2012-01-01

    Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) offers the possibility of accurate, rapid, inexpensive identification of bacteria, fungi, and mycobacteria isolated in clinical microbiology laboratories. The procedures for preanalytic processing of organisms and analysis by MALDI-TOF MS are technically simple and reproducible, and commercial databases and interpretive algorithms are available for the identification of a wide spectrum of clinically significant organisms. Although only limited work has been reported on the use of this technique to identify molds, perform strain typing, or determine antibiotic susceptibility results, these are fruitful areas of promising research. As experience is gained with MALDI-TOF MS, it is expected that the databases will be expanded to resolve many of the current inadequate identifications (eg, no identification, genus-level identification) and algorithms for potential misidentification will be developed. The current lack of Food and Drug Administration approval of any MALDI-TOF MS system for organism identification limits widespread use in the United States. PMID:22795961

  12. Damage identification of a TLP floating wind turbine by meta-heuristic algorithms

    NASA Astrophysics Data System (ADS)

    Ettefagh, M. M.

    2015-12-01

    Damage identification of the offshore floating wind turbine by vibration/dynamic signals is one of the important and new research fields in the Structural Health Monitoring (SHM). In this paper a new damage identification method is proposed based on meta-heuristic algorithms using the dynamic response of the TLP (Tension-Leg Platform) floating wind turbine structure. The Genetic Algorithms (GA), Artificial Immune System (AIS), Particle Swarm Optimization (PSO), and Artificial Bee Colony (ABC) are chosen for minimizing the object function, defined properly for damage identification purpose. In addition to studying the capability of mentioned algorithms in correctly identifying the damage, the effect of the response type on the results of identification is studied. Also, the results of proposed damage identification are investigated with considering possible uncertainties of the structure. Finally, for evaluating the proposed method in real condition, a 1/100 scaled experimental setup of TLP Floating Wind Turbine (TLPFWT) is provided in a laboratory scale and the proposed damage identification method is applied to the scaled turbine.

  13. Radio frequency identification-enabled capabilities in a healthcare context: An exploratory study.

    PubMed

    Hornyak, Rob; Lewis, Mark; Sankaranarayan, Balaji

    2016-09-01

    Increasingly, the adoption and use of radio frequency identification systems in hospital settings is gaining prominence. However, despite the transformative impact that radio frequency identification has in healthcare settings, few studies have examined how and why this change may occur. The purpose of this study is to systematically understand how radio frequency identification can transform work practices in an operational process that directly impacts cost and operational efficiency and indirectly contributes to impacting patient safety and quality of care. We leverage an interdisciplinary framework to explore the contextual characteristics that shape the assimilation of radio frequency identification in healthcare settings. By linking the use of radio frequency identification with specific contextual dimensions in healthcare settings, we provide a data-driven account of how and why radio frequency identification can be useful in inventory management in this setting. In doing so, we also contribute to recent work by information systems scholars who argue for a reconfiguration of conventional assumptions regarding the role of technology in contemporary organizations. © The Author(s) 2015.

  14. The identification of living persons on images: A literature review.

    PubMed

    Gibelli, D; Obertová, Z; Ritz-Timme, S; Gabriel, P; Arent, T; Ratnayake, M; De Angelis, D; Cattaneo, C

    2016-03-01

    Personal identification in the forensic context commonly concerns unknown decedents. However, recently there has been an increase in cases which require identification of living persons, especially from surveillance systems. These cases bring about a relatively new challenge for forensic anthropologists and pathologists concerning the selection of the most suitable methodological approaches with regard to the limitations of the photographic representation of a given person for individualization and identity. Facial features are instinctively the primary focus for identification approaches. However, other body parts (e.g. hands), and body height and gait (on videos) have been considered in cases of personal identification. This review aims at summarizing the state-of-the-art concerning the identification of the living on images and videos, including a critical evaluation of the advantages and limitations of different methods. Recommendations are given in order to aid forensic practitioners who face cases of identification of living persons. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  15. THE ROLE OF FORENSIC DENTIST FOLLOWING MASS DISASTER

    PubMed Central

    Kolude, B.; Adeyemi, B.F.; Taiwo, J.O.; Sigbeku, O.F.; Eze, U.O.

    2010-01-01

    This review article focuses on mass disaster situations that may arise from natural or manmade circumstances and the significant role of forensic dental personnel in human identification following such occurrences. The various forensic dental modalities of identification that include matching techniques, postmortem profiling, genetic fingerprinting, dental fossil assessment and dental biometrics with digital subtraction were considered. The varying extent of use of forensic dental techniques and the resulting positive impact on human identification were considered. The importance of preparation by way of special training for forensic dental personnel, mock disaster rehearsal, and use of modern day technology was stressed. The need for international standardization of identification through the use of Interpol Disaster Victim Identification (DVI) for ms was further emphasized. Recommendations for improved human identification in Nigerian situation include reform of the National Emergency Management Association (NEMA), incorporation of dental care in primary health care to facilitate proper ante mortem database of the populace and commencement of identification at site of disaster. PMID:25161478

  16. Oligonucleotide (GTG)5 as a marker for Mycobacterium tuberculosis strain identification.

    PubMed Central

    Wiid, I J; Werely, C; Beyers, N; Donald, P; van Helden, P D

    1994-01-01

    Culture of Mycobacterium tuberculosis provides no information on the identity of a strain or the distribution of such a strain in the community. Strain identification of M. tuberculosis can help to address important epidemiological questions, e.g., the origin of an infection in a patient's household or community, whether reactivation of infection is endogenous or exogenous in origin, and the spread and early detection of organisms with acquired antibiotic resistance. To research this problem, strain identification must be reliable and accurate. Although genetic identification techniques already exist, it is valuable to have genetic identification techniques based on a number of genetic markers to improve the accurate identification of M. tuberculosis strains. We show that oligonucleotide (GTG)5 can be successfully applied to the identification of M. tuberculosis strains. This technique may be particularly useful in cases in which M. tuberculosis strains have few or no insertion elements (e.g., IS6110) or in identifying other strains of mycobacteria when informative probes are lacking. Images PMID:7914207

  17. The role of identification in dynamic psychiatry and psychotherapy.

    PubMed

    Corradi, Richard B

    2011-01-01

    Identification-a psychic process in which a person takes on characteristics of another-is a concept important to the understanding of human nature. It plays an important role in how our personalities develop, in our ability to deal with life's stresses, and in how we interact with other people. Knowledge of its manifestations is essential to dynamic psychiatry and to its applications in psychotherapy. This article defines identification and reviews its role in development and as a defense. It discusses its role in the psychopathology of disorders commonly encountered in psychotherapy practice-depression and anxiety states reactive to losses in life, and borderline states. Clinical vignettes illustrate how identification functions in these conditions, and also how identifications reveal themselves in the transference and are utilized in psychotherapy. A teaching vignette illustrates how important it is that residents learning the art of psychotherapy appreciate the therapeutic potential of identification. The article maintains that, although it often goes unrecognized, identification with the therapist is one of the most effective therapeutic devices in the transference.

  18. Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria

    PubMed Central

    Chui, Huixia; Domish, Larissa; Hernandez, Drexler; Wang, Gehua

    2016-01-01

    Identification and typing of bacteria occupy a large fraction of time and work in clinical microbiology laboratories. With the certification of some MS platforms in recent years, more applications and tests of MS‐based diagnosis methods for bacteria identification and typing have been created, not only on well‐accepted MALDI‐TOF‐MS‐based fingerprint matches, but also on solving the insufficiencies of MALDI‐TOF‐MS‐based platforms and advancing the technology to areas such as targeted MS identification and typing of bacteria, bacterial toxin identification, antibiotics susceptibility/resistance tests, and MS‐based diagnostic method development on unique bacteria such as Clostridium and Mycobacteria. This review summarizes the recent development in MS platforms and applications in bacteria identification and typing of common pathogenic bacteria. PMID:26751976

  19. The confidence-accuracy relationship in eyewitness identification: effects of lineup instructions, foil similarity, and target-absent base rates.

    PubMed

    Brewer, Neil; Wells, Gary L

    2006-03-01

    Discriminating accurate from mistaken eyewitness identifications is a major issue facing criminal justice systems. This study examined whether eyewitness confidence assists such decisions under a variety of conditions using a confidence-accuracy (CA) calibration approach. Participants (N = 1,200) viewed a simulated crime and attempted 2 separate identifications from 8-person target-present or target-absent lineups. Confidence and accuracy were calibrated for choosers (but not nonchoosers) for both targets under all conditions. Lower overconfidence was associated with higher diagnosticity, lower target-absent base rates, and shorter identification latencies. Although researchers agree that courtroom expressions of confidence are uninformative, our findings indicate that confidence assessments obtained immediately after a positive identification can provide a useful guide for investigators about the likely accuracy of an identification.

  20. Schematic and realistic biological motion identification in children with high-functioning autism spectrum disorder

    PubMed Central

    Wright, Kristyn; Kelley, Elizabeth; Poulin-Dubois, Diane

    2014-01-01

    Research investigating biological motion perception in children with ASD has revealed conflicting findings concerning whether impairments in biological motion perception exist. The current study investigated how children with high-functioning ASD (HF-ASD) performed on two tasks of biological motion identification: a novel schematic motion identification task and a point-light biological motion identification task. Twenty-two HFASD children were matched with 21 TD children on gender, non-verbal mental, and chronological, age (M years = 6.72). On both tasks, HF-ASD children performed with similar accuracy as TD children. Across groups, children performed better on animate than on inanimate trials of both tasks. These findings suggest that HF-ASD children's identification of both realistic and schematic biological motion identification is unimpaired. PMID:25395988

  1. Identification Male Fertility Through Abnormalities Sperm Based Morphology (Teratospermia) using Invariant Moment Method

    NASA Astrophysics Data System (ADS)

    Syahputra, M. F.; Chairani, R.; Seniman; Rahmat, R. F.; Abdullah, D.; Napitupulu, D.; Setiawan, M. I.; Albra, W.; Erliana, C. I.; Andayani, U.

    2018-03-01

    Sperm morphology is still a standard laboratory analysis in diagnosing infertility in men. Manually identification of sperm form is still not accurate, the difficulty in seeing the form of the invisible sperm from the digital microscope image is often a weakness in the process of identification and takes a long time. Therefore, male fertility identification application system is needed Through sperm abnormalities based on sperm morphology (teratospermia). The method used is invariant moment method. This study uses 15 data testing and 20 data training sperm image. That the process of male fertility identification through sperm abnormalities based on sperm morphology (teratospermia) has an accuracy rate of 80.77%. Use of time to process Identification of male fertility through sperm abnormalities Based on sperm morphology (teratospermia) during 0.4369 seconds.

  2. [Research on airborne hyperspectral identification of red tide organism dominant species based on SVM].

    PubMed

    Ma, Yi; Zhang, Jie; Cui, Ting-wei

    2006-12-01

    Airborne hyperspectral identification of red tide organism dominant species can provide technique for distinguishing red tide and its toxin, and provide support for scaling the disaster. Based on support vector machine(SVM), the present paper provides an identification model of red tide dominant species. Utilizing this model, the authors accomplished three identification experiments with the hyperspectral data obtained on 16th July, and 19th and 25th August, 2001. It is shown from the identification results that the model has a high precision and is not restricted by high dimension of the hyperspectral data.

  3. Idols as Sunshine or Road Signs: Comparing Absorption-Addiction Idolatry With Identification-Emulation Idolatry.

    PubMed

    Cheung, Chau-Kiu; Yue, Xiao Dong

    2018-01-01

    This study seeks to contrast absorption-addiction idolatry and identification-emulation idolatry. Whereas absorption-addiction idolatry progresses from entertainment/socializing to personalizing and obsession about the idol, identification-emulation idolatry unfolds in terms of identification, attachment, romantization, idealization, and consumption about the idol or his or her derivatives. Based on a sample of 1310 secondary school and university students in Hong Kong, the study verified the original factor model composed of five first-order identification-emulation idolatry and three first-order absorption-addiction idolatry factors, with the latter more predictable by fans' club membership.

  4. 9 CFR 55.25 - Animal identification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... CWD National Database or in an approved State database. The second animal identification must be... CWD National Database or in an approved State database. The means of animal identification must be...

  5. 9 CFR 55.25 - Animal identification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... CWD National Database or in an approved State database. The second animal identification must be... CWD National Database or in an approved State database. The means of animal identification must be...

  6. 32 CFR 707.7 - Submarine identification light.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... RULES WITH RESPECT TO ADDITIONAL STATION AND SIGNAL LIGHTS § 707.7 Submarine identification light. Submarines may display, as a distinctive means of identification, an intermittent flashing amber beacon with...

  7. 5 CFR 2100.4 - Requirements for identification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... must provide their full name and as much information as possible in order that a proper search for..., military or annuitant identification card, or any official document as acceptable identification validation...

  8. 40 CFR 62.7325 - Identification of plan.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Plan for... Identification of plan. (a) Identification of plan. New Hampshire Plan for the Control of Designated Pollutants...

  9. 40 CFR 62.7325 - Identification of plan.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Plan for... Identification of plan. (a) Identification of plan. New Hampshire Plan for the Control of Designated Pollutants...

  10. 40 CFR 62.7325 - Identification of plan.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Plan for... Identification of plan. (a) Identification of plan. New Hampshire Plan for the Control of Designated Pollutants...

  11. 40 CFR 62.7325 - Identification of plan.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Plan for... Identification of plan. (a) Identification of plan. New Hampshire Plan for the Control of Designated Pollutants...

  12. 40 CFR 62.7325 - Identification of plan.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...) APPROVAL AND PROMULGATION OF STATE PLANS FOR DESIGNATED FACILITIES AND POLLUTANTS New Hampshire Plan for... Identification of plan. (a) Identification of plan. New Hampshire Plan for the Control of Designated Pollutants...

  13. Boundary shape identification problems in two-dimensional domains related to thermal testing of materials

    NASA Technical Reports Server (NTRS)

    Banks, H. T.; Kojima, Fumio

    1988-01-01

    The identification of the geometrical structure of the system boundary for a two-dimensional diffusion system is reported. The domain identification problem treated here is converted into an optimization problem based on a fit-to-data criterion and theoretical convergence results for approximate identification techniques are discussed. Results of numerical experiments to demonstrate the efficacy of the theoretical ideas are reported.

  14. Chapter 08: Comments on, and additional information for, wood identification

    Treesearch

    Alex C. Wiedenhoeft

    2011-01-01

    This manual has described the theory of identification (Chapter 1), the botanical basis of wood structure (Chapter 2), the use of a hand lens (Chapter 3), how to use cutting tools to prepare wood for observation with a lens (Chapter 4), and the characters used in hand lens wood identification (Chapter 5) before leading you through an identification key (Chapter 6) and...

  15. Department of Defense (DOD) Automated Biometric Identification System (ABIS) Version 1.2: Initial Operational Test and Evaluation Report

    DTIC Science & Technology

    2015-05-01

    Director, Operational Test and Evaluation Department of Defense (DOD) Automated Biometric Identification System (ABIS) Version 1.2 Initial...Operational Test and Evaluation Report May 2015 This report on the Department of Defense (DOD) Automated Biometric Identification System...COVERED - 4. TITLE AND SUBTITLE Department of Defense (DOD) Automated Biometric Identification System (ABIS) Version 1.2 Initial Operational Test

  16. System Identification of Mistuned Bladed Disks from Traveling Wave Response Measurements

    NASA Technical Reports Server (NTRS)

    Feiner, D. M.; Griffin, J. H.; Jones, K. W.; Kenyon, J. A.; Mehmed, O.; Kurkov, A. P.

    2003-01-01

    A new approach to modal analysis is presented. By applying this technique to bladed disk system identification methods, one can determine the mistuning in a rotor based on its response to a traveling wave excitation. This allows system identification to be performed under rotating conditions, and thus expands the applicability of existing mistuning identification techniques from integrally bladed rotors to conventional bladed disks.

  17. Introjective identification: the analytic work of evocation.

    PubMed

    Eekhoff, Judy K

    2016-12-01

    This paper focuses on a particular counter-transference process-introjective identification and the evocation it enables. Introjective identification enables evocation because it engages the analyst's radical openness to the experience of the patient at the most primordial level. The accumulated wisdom of Ferenczi and those who followed him is used to discuss the role of introjective identification in the treatment of patients with non-neurotic structures.

  18. Evaluating common de-identification heuristics for personal health information.

    PubMed

    El Emam, Khaled; Jabbouri, Sam; Sams, Scott; Drouet, Youenn; Power, Michael

    2006-11-21

    With the growing adoption of electronic medical records, there are increasing demands for the use of this electronic clinical data in observational research. A frequent ethics board requirement for such secondary use of personal health information in observational research is that the data be de-identified. De-identification heuristics are provided in the Health Insurance Portability and Accountability Act Privacy Rule, funding agency and professional association privacy guidelines, and common practice. The aim of the study was to evaluate whether the re-identification risks due to record linkage are sufficiently low when following common de-identification heuristics and whether the risk is stable across sample sizes and data sets. Two methods were followed to construct identification data sets. Re-identification attacks were simulated on these. For each data set we varied the sample size down to 30 individuals, and for each sample size evaluated the risk of re-identification for all combinations of quasi-identifiers. The combinations of quasi-identifiers that were low risk more than 50% of the time were considered stable. The identification data sets we were able to construct were the list of all physicians and the list of all lawyers registered in Ontario, using 1% sampling fractions. The quasi-identifiers of region, gender, and year of birth were found to be low risk more than 50% of the time across both data sets. The combination of gender and region was also found to be low risk more than 50% of the time. We were not able to create an identification data set for the whole population. Existing Canadian federal and provincial privacy laws help explain why it is difficult to create an identification data set for the whole population. That such examples of high re-identification risk exist for mainstream professions makes a strong case for not disclosing the high-risk variables and their combinations identified here. For professional subpopulations with published membership lists, many variables often needed by researchers would have to be excluded or generalized to ensure consistently low re-identification risk. Data custodians and researchers need to consider other statistical disclosure techniques for protecting privacy.

  19. Evaluating Common De-Identification Heuristics for Personal Health Information

    PubMed Central

    Jabbouri, Sam; Sams, Scott; Drouet, Youenn; Power, Michael

    2006-01-01

    Background With the growing adoption of electronic medical records, there are increasing demands for the use of this electronic clinical data in observational research. A frequent ethics board requirement for such secondary use of personal health information in observational research is that the data be de-identified. De-identification heuristics are provided in the Health Insurance Portability and Accountability Act Privacy Rule, funding agency and professional association privacy guidelines, and common practice. Objective The aim of the study was to evaluate whether the re-identification risks due to record linkage are sufficiently low when following common de-identification heuristics and whether the risk is stable across sample sizes and data sets. Methods Two methods were followed to construct identification data sets. Re-identification attacks were simulated on these. For each data set we varied the sample size down to 30 individuals, and for each sample size evaluated the risk of re-identification for all combinations of quasi-identifiers. The combinations of quasi-identifiers that were low risk more than 50% of the time were considered stable. Results The identification data sets we were able to construct were the list of all physicians and the list of all lawyers registered in Ontario, using 1% sampling fractions. The quasi-identifiers of region, gender, and year of birth were found to be low risk more than 50% of the time across both data sets. The combination of gender and region was also found to be low risk more than 50% of the time. We were not able to create an identification data set for the whole population. Conclusions Existing Canadian federal and provincial privacy laws help explain why it is difficult to create an identification data set for the whole population. That such examples of high re-identification risk exist for mainstream professions makes a strong case for not disclosing the high-risk variables and their combinations identified here. For professional subpopulations with published membership lists, many variables often needed by researchers would have to be excluded or generalized to ensure consistently low re-identification risk. Data custodians and researchers need to consider other statistical disclosure techniques for protecting privacy. PMID:17213047

  20. A unified framework for evaluating the risk of re-identification of text de-identification tools.

    PubMed

    Scaiano, Martin; Middleton, Grant; Arbuckle, Luk; Kolhatkar, Varada; Peyton, Liam; Dowling, Moira; Gipson, Debbie S; El Emam, Khaled

    2016-10-01

    It has become regular practice to de-identify unstructured medical text for use in research using automatic methods, the goal of which is to remove patient identifying information to minimize re-identification risk. The metrics commonly used to determine if these systems are performing well do not accurately reflect the risk of a patient being re-identified. We therefore developed a framework for measuring the risk of re-identification associated with textual data releases. We apply the proposed evaluation framework to a data set from the University of Michigan Medical School. Our risk assessment results are then compared with those that would be obtained using a typical contemporary micro-average evaluation of recall in order to illustrate the difference between the proposed evaluation framework and the current baseline method. We demonstrate how this framework compares against common measures of the re-identification risk associated with an automated text de-identification process. For the probability of re-identification using our evaluation framework we obtained a mean value for direct identifiers of 0.0074 and a mean value for quasi-identifiers of 0.0022. The 95% confidence interval for these estimates were below the relevant thresholds. The threshold for direct identifier risk was based on previously used approaches in the literature. The threshold for quasi-identifiers was determined based on the context of the data release following commonly used de-identification criteria for structured data. Our framework attempts to correct for poorly distributed evaluation corpora, accounts for the data release context, and avoids the often optimistic assumptions that are made using the more traditional evaluation approach. It therefore provides a more realistic estimate of the true probability of re-identification. This framework should be used as a basis for computing re-identification risk in order to more realistically evaluate future text de-identification tools. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.

  1. How enhanced molecular ions in Cold EI improve compound identification by the NIST library.

    PubMed

    Alon, Tal; Amirav, Aviv

    2015-12-15

    Library-based compound identification with electron ionization (EI) mass spectrometry (MS) is a well-established identification method which provides the names and structures of sample compounds up to the isomer level. The library (such as NIST) search algorithm compares different EI mass spectra in the library's database with the measured EI mass spectrum, assigning each of them a similarity score called 'Match' and an overall identification probability. Cold EI, electron ionization of vibrationally cold molecules in supersonic molecular beams, provides mass spectra with all the standard EI fragment ions combined with enhanced Molecular Ions and high-mass fragments. As a result, Cold EI mass spectra differ from those provided by standard EI and tend to yield lower matching scores. However, in most cases, library identification actually improves with Cold EI, as library identification probabilities for the correct library mass spectra increase, despite the lower matching factors. This research examined the way that enhanced molecular ion abundances affect library identification probability and the way that Cold EI mass spectra, which include enhanced molecular ions and high-mass fragment ions, typically improve library identification results. It involved several computer simulations, which incrementally modified the relative abundances of the various ions and analyzed the resulting mass spectra. The simulation results support previous measurements, showing that while enhanced molecular ion and high-mass fragment ions lower the matching factor of the correct library compound, the matching factors of the incorrect library candidates are lowered even more, resulting in a rise in the identification probability for the correct compound. This behavior which was previously observed by analyzing Cold EI mass spectra can be explained by the fact that high-mass ions, and especially the molecular ion, characterize a compound more than low-mass ions and therefore carries more weight in library search identification algorithms. These ions are uniquely abundant in Cold EI, which therefore enables enhanced compound characterization along with improved NIST library based identification. Copyright © 2015 John Wiley & Sons, Ltd.

  2. Dental plaque identification at home

    MedlinePlus

    ... this page: //medlineplus.gov/ency/article/003426.htm Dental plaque identification at home To use the sharing ... a sticky substance that collects around and between teeth. The home dental plaque identification test shows where ...

  3. Prospective Evaluation of a Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System in a Hospital Clinical Microbiology Laboratory for Identification of Bacteria and Yeasts: a Bench-by-Bench Study for Assessing the Impact on Time to Identification and Cost-Effectiveness

    PubMed Central

    Tan, K. E.; Ellis, B. C.; Lee, R.; Stamper, P. D.; Zhang, S. X.

    2012-01-01

    Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has been found to be an accurate, rapid, and inexpensive method for the identification of bacteria and yeasts. Previous evaluations have compared the accuracy, time to identification, and costs of the MALDI-TOF MS method against standard identification systems or commercial panels. In this prospective study, we compared a protocol incorporating MALDI-TOF MS (MALDI protocol) with the current standard identification protocols (standard protocol) to determine the performance in actual practice using a specimen-based, bench-by-bench approach. The potential impact on time to identification (TTI) and costs had MALDI-TOF MS been the first-line identification method was quantitated. The MALDI protocol includes supplementary tests, notably for Streptococcus pneumoniae and Shigella, and indications for repeat MALDI-TOF MS attempts, often not measured in previous studies. A total of 952 isolates (824 bacterial isolates and 128 yeast isolates) recovered from 2,214 specimens were assessed using the MALDI protocol. Compared with standard protocols, the MALDI protocol provided identifications 1.45 days earlier on average (P < 0.001). In our laboratory, we anticipate that the incorporation of the MALDI protocol can reduce reagent and labor costs of identification by $102,424 or 56.9% within 12 months. The model included the fixed annual costs of the MALDI-TOF MS, such as the cost of protein standards and instrument maintenance, and the annual prevalence of organisms encountered in our laboratory. This comprehensive cost analysis model can be generalized to other moderate- to high-volume laboratories. PMID:22855510

  4. Improving sensitivity in proteome studies by analysis of false discovery rates for multiple search engines

    PubMed Central

    Jones, Andrew R.; Siepen, Jennifer A.; Hubbard, Simon J.; Paton, Norman W.

    2010-01-01

    Tandem mass spectrometry, run in combination with liquid chromatography (LC-MS/MS), can generate large numbers of peptide and protein identifications, for which a variety of database search engines are available. Distinguishing correct identifications from false positives is far from trivial because all data sets are noisy, and tend to be too large for manual inspection, therefore probabilistic methods must be employed to balance the trade-off between sensitivity and specificity. Decoy databases are becoming widely used to place statistical confidence in results sets, allowing the false discovery rate (FDR) to be estimated. It has previously been demonstrated that different MS search engines produce different peptide identification sets, and as such, employing more than one search engine could result in an increased number of peptides being identified. However, such efforts are hindered by the lack of a single scoring framework employed by all search engines. We have developed a search engine independent scoring framework based on FDR which allows peptide identifications from different search engines to be combined, called the FDRScore. We observe that peptide identifications made by three search engines are infrequently false positives, and identifications made by only a single search engine, even with a strong score from the source search engine, are significantly more likely to be false positives. We have developed a second score based on the FDR within peptide identifications grouped according to the set of search engines that have made the identification, called the combined FDRScore. We demonstrate by searching large publicly available data sets that the combined FDRScore can differentiate between between correct and incorrect peptide identifications with high accuracy, allowing on average 35% more peptide identifications to be made at a fixed FDR than using a single search engine. PMID:19253293

  5. Examining the effectiveness of discriminant function analysis and cluster analysis in species identification of male field crickets based on their calling songs.

    PubMed

    Jaiswara, Ranjana; Nandi, Diptarup; Balakrishnan, Rohini

    2013-01-01

    Traditional taxonomy based on morphology has often failed in accurate species identification owing to the occurrence of cryptic species, which are reproductively isolated but morphologically identical. Molecular data have thus been used to complement morphology in species identification. The sexual advertisement calls in several groups of acoustically communicating animals are species-specific and can thus complement molecular data as non-invasive tools for identification. Several statistical tools and automated identifier algorithms have been used to investigate the efficiency of acoustic signals in species identification. Despite a plethora of such methods, there is a general lack of knowledge regarding the appropriate usage of these methods in specific taxa. In this study, we investigated the performance of two commonly used statistical methods, discriminant function analysis (DFA) and cluster analysis, in identification and classification based on acoustic signals of field cricket species belonging to the subfamily Gryllinae. Using a comparative approach we evaluated the optimal number of species and calling song characteristics for both the methods that lead to most accurate classification and identification. The accuracy of classification using DFA was high and was not affected by the number of taxa used. However, a constraint in using discriminant function analysis is the need for a priori classification of songs. Accuracy of classification using cluster analysis, which does not require a priori knowledge, was maximum for 6-7 taxa and decreased significantly when more than ten taxa were analysed together. We also investigated the efficacy of two novel derived acoustic features in improving the accuracy of identification. Our results show that DFA is a reliable statistical tool for species identification using acoustic signals. Our results also show that cluster analysis of acoustic signals in crickets works effectively for species classification and identification.

  6. Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive.

    PubMed

    Hering, Daniel; Borja, Angel; Jones, J Iwan; Pont, Didier; Boets, Pieter; Bouchez, Agnes; Bruce, Kat; Drakare, Stina; Hänfling, Bernd; Kahlert, Maria; Leese, Florian; Meissner, Kristian; Mergen, Patricia; Reyjol, Yorick; Segurado, Pedro; Vogler, Alfried; Kelly, Martyn

    2018-07-01

    Assessment of ecological status for the European Water Framework Directive (WFD) is based on "Biological Quality Elements" (BQEs), namely phytoplankton, benthic flora, benthic invertebrates and fish. Morphological identification of these organisms is a time-consuming and expensive procedure. Here, we assess the options for complementing and, perhaps, replacing morphological identification with procedures using eDNA, metabarcoding or similar approaches. We rate the applicability of DNA-based identification for the individual BQEs and water categories (rivers, lakes, transitional and coastal waters) against eleven criteria, summarised under the headlines representativeness (for example suitability of current sampling methods for DNA-based identification, errors from DNA-based species detection), sensitivity (for example capability to detect sensitive taxa, unassigned reads), precision of DNA-based identification (knowledge about uncertainty), comparability with conventional approaches (for example sensitivity of metrics to differences in DNA-based identification), cost effectiveness and environmental impact. Overall, suitability of DNA-based identification is particularly high for fish, as eDNA is a well-suited sampling approach which can replace expensive and potentially harmful methods such as gill-netting, trawling or electrofishing. Furthermore, there are attempts to replace absolute by relative abundance in metric calculations. For invertebrates and phytobenthos, the main challenges include the modification of indices and completing barcode libraries. For phytoplankton, the barcode libraries are even more problematic, due to the high taxonomic diversity in plankton samples. If current assessment concepts are kept, DNA-based identification is least appropriate for macrophytes (rivers, lakes) and angiosperms/macroalgae (transitional and coastal waters), which are surveyed rather than sampled. We discuss general implications of implementing DNA-based identification into standard ecological assessment, in particular considering any adaptations to the WFD that may be required to facilitate the transition to molecular data. Copyright © 2018 Elsevier Ltd. All rights reserved.

  7. Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Molds of the Fusarium Genus

    PubMed Central

    Stubbe, Dirk; De Cremer, Koen; Piérard, Denis; Normand, Anne-Cécile; Piarroux, Renaud; Detandt, Monique; Hendrickx, Marijke

    2014-01-01

    The rates of infection with Fusarium molds are increasing, and a diverse number of Fusarium spp. belonging to different species complexes can cause infection. Conventional species identification in the clinical laboratory is time-consuming and prone to errors. We therefore evaluated whether matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a useful alternative. The 289 Fusarium strains from the Belgian Coordinated Collections of Microorganisms (BCCM)/Institute of Hygiene and Epidemiology Mycology (IHEM) culture collection with validated sequence-based identities and comprising 40 species were used in this study. An identification strategy was developed, applying a standardized MALDI-TOF MS assay and an in-house reference spectrum database. In vitro antifungal testing was performed to assess important differences in susceptibility between clinically relevant species/species complexes. We observed that no incorrect species complex identifications were made by MALDI-TOF MS, and 82.8% of the identifications were correct to the species level. This success rate was increased to 91% by lowering the cutoff for identification. Although the identification of the correct species complex member was not always guaranteed, antifungal susceptibility testing showed that discriminating between Fusarium species complexes can be important for treatment but is not necessarily required between members of a species complex. With this perspective, some Fusarium species complexes with closely related members can be considered as a whole, increasing the success rate of correct identifications to 97%. The application of our user-friendly MALDI-TOF MS identification approach resulted in a dramatic improvement in both time and accuracy compared to identification with the conventional method. A proof of principle of our MALDI-TOF MS approach in the clinical setting using recently isolated Fusarium strains demonstrated its validity. PMID:25411180

  8. Prospective evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry system in a hospital clinical microbiology laboratory for identification of bacteria and yeasts: a bench-by-bench study for assessing the impact on time to identification and cost-effectiveness.

    PubMed

    Tan, K E; Ellis, B C; Lee, R; Stamper, P D; Zhang, S X; Carroll, K C

    2012-10-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been found to be an accurate, rapid, and inexpensive method for the identification of bacteria and yeasts. Previous evaluations have compared the accuracy, time to identification, and costs of the MALDI-TOF MS method against standard identification systems or commercial panels. In this prospective study, we compared a protocol incorporating MALDI-TOF MS (MALDI protocol) with the current standard identification protocols (standard protocol) to determine the performance in actual practice using a specimen-based, bench-by-bench approach. The potential impact on time to identification (TTI) and costs had MALDI-TOF MS been the first-line identification method was quantitated. The MALDI protocol includes supplementary tests, notably for Streptococcus pneumoniae and Shigella, and indications for repeat MALDI-TOF MS attempts, often not measured in previous studies. A total of 952 isolates (824 bacterial isolates and 128 yeast isolates) recovered from 2,214 specimens were assessed using the MALDI protocol. Compared with standard protocols, the MALDI protocol provided identifications 1.45 days earlier on average (P < 0.001). In our laboratory, we anticipate that the incorporation of the MALDI protocol can reduce reagent and labor costs of identification by $102,424 or 56.9% within 12 months. The model included the fixed annual costs of the MALDI-TOF MS, such as the cost of protein standards and instrument maintenance, and the annual prevalence of organisms encountered in our laboratory. This comprehensive cost analysis model can be generalized to other moderate- to high-volume laboratories.

  9. Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi.

    PubMed

    McMullen, Allison R; Wallace, Meghan A; Pincus, David H; Wilkey, Kathy; Burnham, C A

    2016-08-01

    Invasive fungal infections have a high rate of morbidity and mortality, and accurate identification is necessary to guide appropriate antifungal therapy. With the increasing incidence of invasive disease attributed to filamentous fungi, rapid and accurate species-level identification of these pathogens is necessary. Traditional methods for identification of filamentous fungi can be slow and may lack resolution. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a rapid and accurate method for identification of bacteria and yeasts, but a paucity of data exists on the performance characteristics of this method for identification of filamentous fungi. The objective of our study was to evaluate the accuracy of the Vitek MS for mold identification. A total of 319 mold isolates representing 43 genera recovered from clinical specimens were evaluated. Of these isolates, 213 (66.8%) were correctly identified using the Vitek MS Knowledge Base, version 3.0 database. When a modified SARAMIS (Spectral Archive and Microbial Identification System) database was used to augment the version 3.0 Knowledge Base, 245 (76.8%) isolates were correctly identified. Unidentified isolates were subcultured for repeat testing; 71/319 (22.3%) remained unidentified. Of the unidentified isolates, 69 were not in the database. Only 3 (0.9%) isolates were misidentified by MALDI-TOF MS (including Aspergillus amoenus [n = 2] and Aspergillus calidoustus [n = 1]) although 10 (3.1%) of the original phenotypic identifications were not correct. In addition, this methodology was able to accurately identify 133/144 (93.6%) Aspergillus sp. isolates to the species level. MALDI-TOF MS has the potential to expedite mold identification, and misidentifications are rare. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  10. Early identification of microorganisms in blood culture prior to the detection of a positive signal in the BACTEC FX system using matrix-assisted laser desorption/ionization-time of flight mass spectrometry.

    PubMed

    Wang, Ming-Cheng; Lin, Wei-Hung; Yan, Jing-Jou; Fang, Hsin-Yi; Kuo, Te-Hui; Tseng, Chin-Chung; Wu, Jiunn-Jong

    2015-08-01

    Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is a valuable method for rapid identification of blood stream infection (BSI) pathogens. Integration of MALDI-TOF MS and blood culture system can speed the identification of causative BSI microorganisms. We investigated the minimal microorganism concentrations of common BSI pathogens required for positive blood culture using BACTEC FX and for positive identification using MALDI-TOF MS. The time to detection with positive BACTEC FX and minimal incubation time with positive MALDI-TOF MS identification were determined for earlier identification of common BSI pathogens. The minimal microorganism concentrations required for positive blood culture using BACTEC FX were >10(7)-10(8) colony forming units/mL for most of the BSI pathogens. The minimal microorganism concentrations required for identification using MALDI-TOF MS were > 10(7) colony forming units/mL. Using simulated BSI models, one can obtain enough bacterial concentration from blood culture bottles for successful identification of five common Gram-positive and Gram-negative bacteria using MALDI-TOF MS 1.7-2.3 hours earlier than the usual time to detection in blood culture systems. This study provides an approach to earlier identification of BSI pathogens prior to the detection of a positive signal in the blood culture system using MALDI-TOF MS, compared to current methods. It can speed the time for identification of BSI pathogens and may have benefits of earlier therapy choice and on patient outcome. Copyright © 2013. Published by Elsevier B.V.

  11. 48 CFR 1852.245-74 - Identification and marking of Government equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Technical Handbook (NASA-HDBK) 6003, Application of Data Matrix Identification Symbols to Aerospace Parts... Identification Symbols on Aerospace Parts or through the use of commercial marking techniques that: (1) are...

  12. 48 CFR 1852.245-74 - Identification and marking of Government equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Technical Handbook (NASA-HDBK) 6003, Application of Data Matrix Identification Symbols to Aerospace Parts... Identification Symbols on Aerospace Parts or through the use of commercial marking techniques that: (1) are...

  13. 48 CFR 1852.245-74 - Identification and marking of Government equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Technical Handbook (NASA-HDBK) 6003, Application of Data Matrix Identification Symbols to Aerospace Parts... Identification Symbols on Aerospace Parts or through the use of commercial marking techniques that: (1) are...

  14. 48 CFR 1852.245-74 - Identification and marking of Government equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Technical Handbook (NASA-HDBK) 6003, Application of Data Matrix Identification Symbols to Aerospace Parts... Identification Symbols on Aerospace Parts or through the use of commercial marking techniques that: (1) are...

  15. Role of forensic odontologist in post mortem person identification

    PubMed Central

    Pramod, Jahagirdar B.; Marya, Anand; Sharma, Vidhii

    2012-01-01

    The natural teeth are the most durable organs in the bodies of vertebrates, and humankind's understanding of their own past and evolution relies heavily upon remnant dental evidence found as fossils. The use of features unique to the human dentition as an aid to personal identification is widely accepted within the forensic field. Comparative dental identifications play a major role in identifying the victims of violence, disaster or other mass tragedies. The comparison of ante-mortem and postmortem dental records to determine human identity has long been established. Indeed, it is still a major identification method in criminal investigations, mass disasters, grossly decomposed or traumatized bodies, and in other situations where visual identification is neither possible nor desirable. This article has comprehensively described some of the methods, and additional factors aiding in postmortem person identification. PMID:23559914

  16. Bifocal Stereo for Multipath Person Re-Identification

    NASA Astrophysics Data System (ADS)

    Blott, G.; Heipke, C.

    2017-11-01

    This work presents an approach for the task of person re-identification by exploiting bifocal stereo cameras. Present monocular person re-identification approaches show a decreasing working distance, when increasing the image resolution to obtain a higher reidentification performance. We propose a novel 3D multipath bifocal approach, containing a rectilinear lens with larger focal length for long range distances and a fish eye lens of a smaller focal length for the near range. The person re-identification performance is at least on par with 2D re-identification approaches but the working distance of the approach is increased and on average 10% more re-identification performance can be achieved in the overlapping field of view compared to a single camera. In addition, the 3D information is exploited from the overlapping field of view to solve potential 2D ambiguities.

  17. Oral and Craniofacial Clinical Signs Associated to Genetic Conditions in Human Identification Part I: A Review

    PubMed Central

    Ayoub, Fouad; Aoun, Nicole; el Husseini, Hassan; Jassar, Houssam; Sayah, Fida; Salameh, Ziad

    2015-01-01

    Background: Forensic dentistry is one of the most reliable methods used in human identification when other technique as fingerprint, DNA, visual identification cannot be used. Genetic disorders have several manifestations that can target the intra-oral cavity, the cranio-facial area or any location in the human body. Materials and Methods: A literature search of the scientific database (Medline and Science Direct) for the years 1990 to 2014 was carried out to find out all the available papers that indicate oral, cranio-facial signs, genetic and human identification. Results: A table with 10 genetic conditions was described with oral and cranio-facial signs that can help forensic specialist in human identification. Conclusion: This review showed a correlation between genetics, facial and intra-oral signs that would help forensic ondontologist in the identification procedures. PMID:26028912

  18. Discerning cultural identification from a thinly sliced behavioral sample.

    PubMed

    Hamamura, Takeshi; Li, Liman Man Wai

    2012-12-01

    This research examined whether individual differences in cultural identification can be discerned at zero acquaintance. This issue was examined in Hong Kong, where the idiosyncrasy of cultural identification is a salient social-psychological issue. The participants were able to perceive accurately the targets' identification with Western culture from a video clip and from a still image. Findings also indicated that a stereotype of Western cultural identity (i.e., extraversion and particular hairstyle) facilitated these perceptions. Specifically, (a) the participants with a stronger stereotype were more accurate in perceiving Western cultural identification, (b) the targets who were experimentally manipulated to appear extraverted were rated as more strongly identifying with Western culture, and (c) the participants relatively unfamiliar with these stereotypes did not correctly perceive Western cultural identification. Implications of these findings on research on multiculturalism are also discussed.

  19. Gain-Scheduled Fault Tolerance Control Under False Identification

    NASA Technical Reports Server (NTRS)

    Shin, Jong-Yeob; Belcastro, Christine (Technical Monitor)

    2006-01-01

    An active fault tolerant control (FTC) law is generally sensitive to false identification since the control gain is reconfigured for fault occurrence. In the conventional FTC law design procedure, dynamic variations due to false identification are not considered. In this paper, an FTC synthesis method is developed in order to consider possible variations of closed-loop dynamics under false identification into the control design procedure. An active FTC synthesis problem is formulated into an LMI optimization problem to minimize the upper bound of the induced-L2 norm which can represent the worst-case performance degradation due to false identification. The developed synthesis method is applied for control of the longitudinal motions of FASER (Free-flying Airplane for Subscale Experimental Research). The designed FTC law of the airplane is simulated for pitch angle command tracking under a false identification case.

  20. Fastidious Gram-Negatives: Identification by the Vitek 2 Neisseria-Haemophilus Card and by Partial 16S rRNA Gene Sequencing Analysis.

    PubMed

    Sönksen, Ute Wolff; Christensen, Jens Jørgen; Nielsen, Lisbeth; Hesselbjerg, Annemarie; Hansen, Dennis Schrøder; Bruun, Brita

    2010-12-31

    Taxonomy and identification of fastidious Gram negatives are evolving and challenging. We compared identifications achieved with the Vitek 2 Neisseria-Haemophilus (NH) card and partial 16S rRNA gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic characterization using 100 fastidious Gram negative bacteria. Seventy-five strains represented 21 of the 26 taxa included in the Vitek 2 NH database and 25 strains represented related species not included in the database. Of the 100 strains, 31 were the type strains of the species. Vitek 2 NH identification results: 48 of 75 database strains were correctly identified, 11 strains gave `low discrimination´, seven strains were unidentified, and nine strains were misidentified. Identification of 25 non-database strains resulted in 14 strains incorrectly identified as belonging to species in the database. Partial 16S rRNA gene sequence analysis results: For 76 strains phenotypic and sequencing identifications were identical, for 23 strains the sequencing identifications were either probable or possible, and for one strain only the genus was confirmed. Thus, the Vitek 2 NH system identifies most of the commonly occurring species included in the database. Some strains of rarely occurring species and strains of non-database species closely related to database species cause problems. Partial 16S rRNA gene sequence analysis performs well, but does not always suffice, additional phenotypical characterization being useful for final identification.

  1. Fastidious Gram-Negatives: Identification by the Vitek 2 Neisseria-Haemophilus Card and by Partial 16S rRNA Gene Sequencing Analysis

    PubMed Central

    Sönksen, Ute Wolff; Christensen, Jens Jørgen; Nielsen, Lisbeth; Hesselbjerg, Annemarie; Hansen, Dennis Schrøder; Bruun, Brita

    2010-01-01

    Taxonomy and identification of fastidious Gram negatives are evolving and challenging. We compared identifications achieved with the Vitek 2 Neisseria-Haemophilus (NH) card and partial 16S rRNA gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic characterization using 100 fastidious Gram negative bacteria. Seventy-five strains represented 21 of the 26 taxa included in the Vitek 2 NH database and 25 strains represented related species not included in the database. Of the 100 strains, 31 were the type strains of the species. Vitek 2 NH identification results: 48 of 75 database strains were correctly identified, 11 strains gave `low discrimination´, seven strains were unidentified, and nine strains were misidentified. Identification of 25 non-database strains resulted in 14 strains incorrectly identified as belonging to species in the database. Partial 16S rRNA gene sequence analysis results: For 76 strains phenotypic and sequencing identifications were identical, for 23 strains the sequencing identifications were either probable or possible, and for one strain only the genus was confirmed. Thus, the Vitek 2 NH system identifies most of the commonly occurring species included in the database. Some strains of rarely occurring species and strains of non-database species closely related to database species cause problems. Partial 16S rRNA gene sequence analysis performs well, but does not always suffice, additional phenotypical characterization being useful for final identification. PMID:21347215

  2. Direct identification of bacteria from charcoal-containing blood culture bottles using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.

    PubMed

    Wüppenhorst, N; Consoir, C; Lörch, D; Schneider, C

    2012-10-01

    Several protocols for direct matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) from positive blood cultures are currently used to speed up the diagnostic process of bacteraemia. Identification rates are high and results are accurate for the BACTEC™ system and for charcoal-free bottles. Only a few studies have evaluated protocols for charcoal-containing BacT/ALERT bottles reaching substantially lower identification rates. We established a new protocol for sample preparation from aerobic and anaerobic positive charcoal-containing BacT/ALERT blood culture bottles and measured the protein profiles (n = 167). Then, we integrated this protocol in the routine workflow of our laboratory (n = 212). During the establishment of our protocol, 74.3 % of bacteria were correctly identified to the species level, in 23.4 %, no result and in 2.4 %, a false identification were obtained. Reliable criteria for correct species identification were a score value ≥1.400 and a best match on rank 1-3 of the same species. Identification rates during routine workflow were 77.8 % for correct identification, 20.8 % for not identified samples and 1.4 % for discordant identification. In conclusion, our results indicate that MALDI-TOF MS is possible, even from charcoal-containing blood cultures. Reliable criteria for correct species identification are a score value ≥1.400 and a best match on rank 1-3 of a single species.

  3. Prospective evaluation of the VITEK MS for the routine identification of bacteria and yeast in the clinical microbiology laboratory: assessment of accuracy of identification and turnaround time.

    PubMed

    Charnot-Katsikas, Angella; Tesic, Vera; Boonlayangoor, Sue; Bethel, Cindy; Frank, Karen M

    2014-02-01

    This study assessed the accuracy of bacterial and yeast identification using the VITEK MS, and the time to reporting of isolates before and after its implementation in routine clinical practice. Three hundred and sixty-two isolates of bacteria and yeast, consisting of a variety of clinical isolates and American Type Culture Collection strains, were tested. Results were compared with reference identifications from the VITEK 2 system and with 16S rRNA sequence analysis. The VITEK MS provided an acceptable identification to species level for 283 (78 %) isolates. Considering organisms for which genus-level identification is acceptable for routine clinical care, 315 isolates (87 %) had an acceptable identification. Six isolates (2 %) were identified incorrectly, five of which were Shigella species. Finally, the time for reporting the identifications was decreased significantly after implementation of the VITEK MS for a total mean reduction in time of 10.52 h (P<0.0001). Overall, accuracy of the VITEK MS was comparable or superior to that from the VITEK 2. The findings were also comparable to other studies examining the accuracy of the VITEK MS, although differences exist, depending on the diversity of species represented as well as on the versions of the databases used. The VITEK MS can be incorporated effectively into routine use in a clinical microbiology laboratory and future expansion of the database should provide improved accuracy for the identification of micro-organisms.

  4. Multi-level damage identification with response reconstruction

    NASA Astrophysics Data System (ADS)

    Zhang, Chao-Dong; Xu, You-Lin

    2017-10-01

    Damage identification through finite element (FE) model updating usually forms an inverse problem. Solving the inverse identification problem for complex civil structures is very challenging since the dimension of potential damage parameters in a complex civil structure is often very large. Aside from enormous computation efforts needed in iterative updating, the ill-condition and non-global identifiability features of the inverse problem probably hinder the realization of model updating based damage identification for large civil structures. Following a divide-and-conquer strategy, a multi-level damage identification method is proposed in this paper. The entire structure is decomposed into several manageable substructures and each substructure is further condensed as a macro element using the component mode synthesis (CMS) technique. The damage identification is performed at two levels: the first is at macro element level to locate the potentially damaged region and the second is over the suspicious substructures to further locate as well as quantify the damage severity. In each level's identification, the damage searching space over which model updating is performed is notably narrowed down, not only reducing the computation amount but also increasing the damage identifiability. Besides, the Kalman filter-based response reconstruction is performed at the second level to reconstruct the response of the suspicious substructure for exact damage quantification. Numerical studies and laboratory tests are both conducted on a simply supported overhanging steel beam for conceptual verification. The results demonstrate that the proposed multi-level damage identification via response reconstruction does improve the identification accuracy of damage localization and quantization considerably.

  5. An intervention to decrease patient identification band errors in a children's hospital.

    PubMed

    Hain, Paul D; Joers, B; Rush, M; Slayton, J; Throop, P; Hoagg, S; Allen, L; Grantham, J; Deshpande, J K

    2010-06-01

    Patient misidentification continues to be a quality and safety issue. There is a paucity of US data describing interventions to reduce identification band error rates. Monroe Carell Jr Children's Hospital at Vanderbilt. Percentage of patients with defective identification bands. Web-based surveys were sent, asking hospital personnel to anonymously identify perceived barriers to reaching zero defects with identification bands. Corrective action plans were created and implemented with ideas from leadership, front-line staff and the online survey. Data from unannounced audits of patient identification bands were plotted on statistical process control charts and shared monthly with staff. All hospital personnel were expected to "stop the line" if there were any patient identification questions. The first audit showed a defect rate of 20.4%. The original mean defect rate was 6.5%. After interventions and education, the new mean defect rate was 2.6%. (a) The initial rate of patient identification band errors in the hospital was higher than expected. (b) The action resulting in most significant improvement was staff awareness of the problem, with clear expectations to immediately stop the line if a patient identification error was present. (c) Staff surveys are an excellent source of suggestions for combating patient identification issues. (d) Continued audit and data collection is necessary for sustainable staff focus and continued improvement. (e) Statistical process control charts are both an effective method to track results and an easily understood tool for sharing data with staff.

  6. [Problems in organization of medical criminological registration and personality identification for subjects occupationally exposed to life risk].

    PubMed

    Shcherbakov, V V

    2000-01-01

    The paper discusses problems in organization of identification studies under conditions of mass deaths as exemplified by forensic medical records of medical criminological identification studies of subjects killed during war conflict in Chechnya. The evolution of the organization model of identification studies is shown transformation of organization philosophy, formation of expert algorithms, formalization and technologic realization of expert solutions.

  7. Electro-Optic Identification (EOID) Research Program

    DTIC Science & Technology

    2002-09-30

    The goal of this research is to provide computer-assisted identification of underwater mines in electro - optic imagery. Identification algorithms will...greatly reduce the time and risk to reacquire mine-like-objects for positive classification and identification. The objectives are to collect electro ... optic data under a wide range of operating and environmental conditions and develop precise algorithms that can provide accurate target recognition on this data for all possible conditions.

  8. Comparison of identification methods for oral asaccharolytic Eubacterium species.

    PubMed

    Wade, W G; Slayne, M A; Aldred, M J

    1990-12-01

    Thirty one strains of oral, asaccharolytic Eubacterium spp. and the type strains of E. brachy, E. nodatum and E. timidum were subjected to three identification techniques--protein-profile analysis, determination of metabolic end-products, and the API ATB32A identification kit. Five clusters were obtained from numerical analysis of protein profiles and excellent correlations were seen with the other two methods. Protein profiles alone allowed unequivocal identification.

  9. Studies of beta Coronae Borealis. II - Identification of the lanthanides

    NASA Technical Reports Server (NTRS)

    Adelman, S. J.; Shore, S. N.; Tiernan, M. F.

    1973-01-01

    A table presented contains the identification of Ce III (Sugar 1965), Pr III (Sugar 1961), Nd III (Crosswhite 1972), Sm III (Crosswhite 1969), Ho II, and Yb II (Corliss and Tech 1972) lines. The tolerance for identifications was 0.04 A. Each of these ionic identifications is based primarily on only a few of the lines listed while the remaining lines which are normally blended add support.

  10. Improving sensitivity in proteome studies by analysis of false discovery rates for multiple search engines.

    PubMed

    Jones, Andrew R; Siepen, Jennifer A; Hubbard, Simon J; Paton, Norman W

    2009-03-01

    LC-MS experiments can generate large quantities of data, for which a variety of database search engines are available to make peptide and protein identifications. Decoy databases are becoming widely used to place statistical confidence in result sets, allowing the false discovery rate (FDR) to be estimated. Different search engines produce different identification sets so employing more than one search engine could result in an increased number of peptides (and proteins) being identified, if an appropriate mechanism for combining data can be defined. We have developed a search engine independent score, based on FDR, which allows peptide identifications from different search engines to be combined, called the FDR Score. The results demonstrate that the observed FDR is significantly different when analysing the set of identifications made by all three search engines, by each pair of search engines or by a single search engine. Our algorithm assigns identifications to groups according to the set of search engines that have made the identification, and re-assigns the score (combined FDR Score). The combined FDR Score can differentiate between correct and incorrect peptide identifications with high accuracy, allowing on average 35% more peptide identifications to be made at a fixed FDR than using a single search engine.

  11. Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci

    PubMed Central

    Bascomb, Shoshana; Manafi, Mammad

    1998-01-01

    The contribution of enzyme tests to the accurate and rapid routine identification of gram-positive cocci is introduced. The current taxonomy of the genera of aerobic and facultatively anaerobic cocci based on genotypic and phenotypic characterization is reviewed. The clinical and economic importance of members of these taxa is briefly summarized. Tables summarizing test schemes and kits available for the identification of staphylococci, enterococci, and streptococci on the basis of general requirements, number of tests, number of taxa, test classes, and completion times are discussed. Enzyme tests included in each scheme are compared on the basis of their synthetic moiety. The current understanding of the activity of enzymes important for classification and identification of the major groups, methods of testing, and relevance to the ease and speed of identification are reviewed. Publications describing the use of different identification kits are listed, and overall identification successes and problems are discussed. The relationships between the results of conventional biochemical and rapid enzyme tests are described and considered. The use of synthetic substrates for the detection of glycosidases and peptidases is reviewed, and the advantages of fluorogenic synthetic moieties are discussed. The relevance of enzyme tests to accurate and meaningful rapid routine identification is discussed. PMID:9564566

  12. Forensic dentistry in human identification: A review of the literature.

    PubMed

    Ata-Ali, Javier; Ata-Ali, Fadi

    2014-04-01

    An update is provided of the literature on the role of odontology in human identification, based on a PubMed-Medline search of the last 5 years and using the terms: "forensic dentistry" (n = 464 articles), "forensic odontology" (n = 141 articles) and "forensic dentistry identification" (n = 169 articles). Apart from these initial 774 articles, others considered to be important and which were generated by a manual search and cited as references in review articles were also included. Forensic dentistry requires interdisciplinary knowledge, since the data obtained from the oral cavity can contribute to identify an individual or provide information needed in a legal process. Furthermore, the data obtained from the oral cavity can narrow the search range of an individual and play a key role in the victim identification process following mass disasters or catastrophes. This literature search covering the last 5 years describes the novelties referred to buccodental studies in comparative identification, buccodental evaluation in reconstructive identification, human bites as a method for identifying the aggressor, and the role of DNA in dental identification. The oral cavity is a rich and noninvasive source of DNA, and can be used to solve problems of a social, economic or legal nature. Key words:Forensic identification, DNA, forensic dentistry, rugoscopy, cheiloscopy, saliva.

  13. Predictability of physiological testing and the role of maturation in talent identification for adolescent team sports.

    PubMed

    Pearson, D T; Naughton, G A; Torode, M

    2006-08-01

    Entrepreneurial marketing of sport increases demands on sport development officers to identify talented individuals for specialist development at the youngest possible age. Talent identification results in the streamlining of resources to produce optimal returns from a sports investment. However, the process of talent identification for team sports is complex and success prediction is imperfect. The aim of this review is to describe existing practices in physiological tests used for talent identification in team sports and discuss the impact of maturity-related differences on the long term outcomes particularly for male participants. Maturation is a major confounding variable in talent identification during adolescence. A myriad of hormonal changes during puberty results in physical and physiological characteristics important for sporting performance. Significant changes during puberty make the prediction of adult performance difficult from adolescent data. Furthermore, for talent identification programs to succeed, valid and reliable testing procedures must be accepted and implemented in a range of performance-related categories. Limited success in scientifically based talent identification is evident in a range of team sports. Genetic advances challenge the ethics of talent identification in adolescent sport. However, the environment remains a significant component of success prediction in sport. Considerations for supporting talented young male athletes are discussed.

  14. Identification with the retail organization and customer-perceived employee similarity: effects on customer spending.

    PubMed

    Netemeyer, Richard G; Heilman, Carrie M; Maxham, James G

    2012-09-01

    Two constructs important to academicians and managers are the degree to which employees and customers identify with an organization, employee organizational identification (employee OI) and customer-company identification (customer identification), respectively. This research examines the effects of these identification constructs and the related construct of customer perceived similarity to employees on customer spending. Via a 1-year multilevel study of 12,047 customers and 1,464 store employees (sales associates) covering 212 stores of a specialty apparel retailer, our study contributes to the literature in 2 critical ways. First, we expand the theoretical network of employee OI and customer identification by examining the related construct of a customer's perceived similarity to store employees. We examine the incremental (not fully mediated) main and interaction effects of customer-perceived similarity to employees and employee OI on customer spending. Second, we examine the effect of customer identification on customer spending relative to the effect of customer satisfaction on customer spending. Thus, our study also contributes by demonstrating a potential complementary route to achieve customer spending (customer identification), a route that may be more readily affected by management than the efforts required for a sustained increase in customer satisfaction. Implications for academics and managers are offered.

  15. The effects of authentic leadership, organizational identification, and occupational coping self-efficacy on new graduate nurses' job turnover intentions in Canada.

    PubMed

    Fallatah, Fatmah; Laschinger, Heather K S; Read, Emily A

    Nurses' turnover has a costly impact on organizations, patients, and nurses. Numerous studies have highlighted the critical role of nursing leadership in enhancing new nurses' retention. To examine the influence of authentic leadership on new nurses' job turnover intentions through their personal identification with the leader, organizational identification, and occupational coping self-efficacy. Secondary data analysis of a cross-sectional national study of Canadian new graduate nurses was conducted using structural equation modeling. Authentic leadership had a significant positive effect on nurses' personal identification with their leader and their organization. Personal identification mediated the relationship between authentic leadership and organizational identification. Organizational identification had a significant positive effect on occupational coping self-efficacy, which, in turn, had a negative effect on new graduate nurses' job turnover intentions. The findings demonstrate the vital role authentic leadership plays in retaining new graduate nurses. Authentic leaders foster personal and organizational identification among new graduate nurses, leading to increase in the confidence in their ability to manage work-related challenges, which subsequently results in positive outcomes in both new graduate nurses and the organization. Copyright © 2016 Elsevier Inc. All rights reserved.

  16. Bird on Your Smartphone: How to make identification faster?

    NASA Astrophysics Data System (ADS)

    Hidayat, T.; Kurniawan, I. S.; Tapilow, F. S.

    2018-01-01

    Identification skills of students are needed in the field activities of animal ecology course. Good identification skills will help students to understand the traits, determine differences and similarities in order to naming of birds’ species. This study aims to describe the identification skill of students by using smart phone applications designed in such a way as a support in the field activities. Research method used was quasi experiment involving 60 students which were divided into two groups, one group that use smartphone applications (SA) and other group using a guidebook (GB). This study was carried out in the classroom and outside (the field). Instruments used in this research included tests and questionnaire. The identification skills were measured by tests, indicated by an average score (AS). The results showed that the identification skills of SA students were higher (AS = 3.12) than those of GB one (AS = 2.91). These results are in accordance with response of students. The most of students (90.08%) mentioned that the use of smart phone applications in identifying birds is helpful, more effective and convenience to make identification faster. For further implementation, however, performance of the smartphone used here need to be enhanced to improve the identification skills of students and for wider use.

  17. Agent tracking: a psycho-historical theory of the identification of living and social agents.

    PubMed

    Bullot, Nicolas J

    To explain agent-identification behaviours, universalist theories in the biological and cognitive sciences have posited mental mechanisms thought to be universal to all humans, such as agent detection and face recognition mechanisms. These universalist theories have paid little attention to how particular sociocultural or historical contexts interact with the psychobiological processes of agent-identification. In contrast to universalist theories, contextualist theories appeal to particular historical and sociocultural contexts for explaining agent-identification. Contextualist theories tend to adopt idiographic methods aimed at recording the heterogeneity of human behaviours across history, space, and cultures. Defenders of the universalist approach tend to criticise idiographic methods because such methods can lead to relativism or may lack generality. To overcome explanatory limitations of proposals that adopt either universalist or contextualist approaches in isolation, I propose a philosophical model that integrates contributions from both traditions: the psycho-historical theory of agent-identification. This theory investigates how the tracking processes that humans use for identifying agents interact with the unique socio-historical contexts that support agent-identification practices. In integrating hypotheses about the history of agents with psychological and epistemological principles regarding agent-identification, the theory can generate novel hypotheses regarding the distinction between recognition-based, heuristic-based, and explanation-based agent-identification.

  18. Identification and molecular epidemiology of dermatophyte isolates by repetitive-sequence-PCR-based DNA fingerprinting using the DiversiLab system in Turkey.

    PubMed

    Koc, A Nedret; Atalay, Mustafa A; Inci, Melek; Sariguzel, Fatma M; Sav, Hafize

    2017-05-01

    Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux). The genotyping of dermatophyte isolates from different patients was determined by rep-PCR. In the identification of dermatophyte isolates, agreement between rep-PCR and conventional methods was 87.8 % ( 36 of 41). The dermatophyte strains belonged to four clones (A -D) which were determined by the use of rep-PCR. The dermatophyte strains in Clone B, D showed identical patterns with respect to the region. In conclusion, rep-PCR appears to be useful for evaluation of the identification and clonal relationships between Trichophyton rubrum species complex and Trichophyton mentagrophytes species complex isolates. The similarity and diversity of these isolates may be assessed according to different regions by rep-PCR. © 2017 Blackwell Verlag GmbH.

  19. Determination of new retention indices for quick identification of essential oils compounds.

    PubMed

    Hérent, Marie-France; De Bie, Véronique; Tilquin, Bernard

    2007-02-19

    The classical methods of chromatographic identification of compounds were based on calculation of retention indices by using different stationary phases. The aim of the work was to differentiate essential oils extracted from different plant species by identification of some of their major compounds. The method of identification was based on the calculation of new retention indices of essential oils compounds fractionated on a polar chromatographic column with temperature programming system. Similar chromatograms have been obtained on the same column for one plant family with two different temperature gradients allowing the rapid identification of essential oils of different species, sub-species or chemotypes of Citrus, Mentha and Thymus.

  20. Crop Identification Technolgy Assessment for Remote Sensing (CITARS). Volume 1: Task design plan

    NASA Technical Reports Server (NTRS)

    Hall, F. G.; Bizzell, R. M.

    1975-01-01

    A plan for quantifying the crop identification performances resulting from the remote identification of corn, soybeans, and wheat is described. Steps for the conversion of multispectral data tapes to classification results are specified. The crop identification performances resulting from the use of several basic types of automatic data processing techniques are compared and examined for significant differences. The techniques are evaluated also for changes in geographic location, time of the year, management practices, and other physical factors. The results of the Crop Identification Technology Assessment for Remote Sensing task will be applied extensively in the Large Area Crop Inventory Experiment.

  1. DNA barcoding and morphological identification of neotropical ichthyoplankton from the Upper Paraná and São Francisco.

    PubMed

    Becker, R A; Sales, N G; Santos, G M; Santos, G B; Carvalho, D C

    2015-07-01

    The identification of fish larvae from two neotropical hydrographic basins using traditional morphological taxonomy and DNA barcoding revealed no conflicting results between the morphological and barcode identification of larvae. A lower rate (25%) of correct morphological identification of eggs as belonging to migratory or non-migratory species was achieved. Accurate identification of ichthyoplankton by DNA barcoding is an important tool for fish reproductive behaviour studies, correct estimation of biodiversity by detecting eggs from rare species, as well as defining environmental and management strategies for fish conservation in the neotropics. © 2015 The Fisheries Society of the British Isles.

  2. New results on finite-time parameter identification and synchronization of uncertain complex dynamical networks with perturbation

    NASA Astrophysics Data System (ADS)

    Zhao, Hui; Zheng, Mingwen; Li, Shudong; Wang, Weiping

    2018-03-01

    Some existing papers focused on finite-time parameter identification and synchronization, but provided incomplete theoretical analyses. Such works incorporated conflicting constraints for parameter identification, therefore, the practical significance could not be fully demonstrated. To overcome such limitations, the underlying paper presents new results of parameter identification and synchronization for uncertain complex dynamical networks with impulsive effect and stochastic perturbation based on finite-time stability theory. Novel results of parameter identification and synchronization control criteria are obtained in a finite time by utilizing Lyapunov function and linear matrix inequality respectively. Finally, numerical examples are presented to illustrate the effectiveness of our theoretical results.

  3. Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems.

    PubMed

    Justesen, Ulrik Stenz; Holm, Anette; Knudsen, Elisa; Andersen, Line Bisgaard; Jensen, Thøger Gorm; Kemp, Michael; Skov, Marianne Nielsine; Gahrn-Hansen, Bente; Møller, Jens Kjølseth

    2011-12-01

    We compared two matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Shimadzu/SARAMIS and Bruker) on a collection of consecutive clinically important anaerobic bacteria (n = 290). The Bruker system had more correct identifications to the species level (67.2% versus 49.0%), but also more incorrect identifications (7.9% versus 1.4%). The system databases need to be optimized to increase identification levels. However, MALDI-TOF MS in its present version seems to be a fast and inexpensive method for identification of most clinically important anaerobic bacteria.

  4. Contextual information renders experts vulnerable to making erroneous identifications.

    PubMed

    Dror, Itiel E; Charlton, David; Péron, Ailsa E

    2006-01-06

    We investigated whether experts can objectively focus on feature information in fingerprints without being misled by extraneous information, such as context. We took fingerprints that have previously been examined and assessed by latent print experts to make positive identification of suspects. Then we presented these same fingerprints again, to the same experts, but gave a context that suggested that they were a no-match, and hence the suspects could not be identified. Within this new context, most of the fingerprint experts made different judgements, thus contradicting their own previous identification decisions. Cognitive aspects involved in biometric identification can explain why experts are vulnerable to make erroneous identifications.

  5. Stochastic system identification in structural dynamics

    USGS Publications Warehouse

    Safak, Erdal

    1988-01-01

    Recently, new identification methods have been developed by using the concept of optimal-recursive filtering and stochastic approximation. These methods, known as stochastic identification, are based on the statistical properties of the signal and noise, and do not require the assumptions of current methods. The criterion for stochastic system identification is that the difference between the recorded output and the output from the identified system (i.e., the residual of the identification) should be equal to white noise. In this paper, first a brief review of the theory is given. Then, an application of the method is presented by using ambient vibration data from a nine-story building.

  6. Biometric identification based on feature fusion with PCA and SVM

    NASA Astrophysics Data System (ADS)

    Lefkovits, László; Lefkovits, Szidónia; Emerich, Simina

    2018-04-01

    Biometric identification is gaining ground compared to traditional identification methods. Many biometric measurements may be used for secure human identification. The most reliable among them is the iris pattern because of its uniqueness, stability, unforgeability and inalterability over time. The approach presented in this paper is a fusion of different feature descriptor methods such as HOG, LIOP, LBP, used for extracting iris texture information. The classifiers obtained through the SVM and PCA methods demonstrate the effectiveness of our system applied to one and both irises. The performances measured are highly accurate and foreshadow a fusion system with a rate of identification approaching 100% on the UPOL database.

  7. "I want to be a daddy!": meanings of masculine identifications in girls.

    PubMed

    Rees, K

    1987-07-01

    This paper suggests revisions in our understanding of feminine identity formation, the girl's negative oedipus complex, and masculine identifications in girls. Analytic material from the cases of three girls is used to explore the various origins and intrapsychic functions of masculine identifications at each phase of the girl's development. Accounts of feminine development based on such concepts as castration shock, primary femininity, primary identification with mother, or core gender identity are seen as oversimplified. Feminine and masculine identifications are neither primary nor secondary, but the product of a long line of development, of ongoing conflict resolution, and of defensive transformations.

  8. [Impact of interactivity on identification with characters in fiction].

    PubMed

    Soto-Sanfiel, María T; Aymerich-Franch, Laura; Ribes Guàrdia, Francesc Xavier

    2010-11-01

    The effect of interactivity on identification with characters in audiovisual fiction was observed. 310 participants were asked to watch a film in one of these two conditions: 1) interactive (they selected the plot), and 2) non-interactive (they consumed the fiction in a conventional way). After watching the movie, they completed a questionnaire with the EDI scale of identification and empathy with characters, created by Igartua and Paez. The capacity to intervene in the configuration of the plot (interactivity) affected identification with characters. The results provide data about the psychology of media and interactivity in communication and allow us to understand the processes of empathy and identification with characters.

  9. 48 CFR 211.275 - Radio frequency identification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Radio frequency identification. 211.275 Section 211.275 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... Requirements Documents 211.275 Radio frequency identification. ...

  10. 49 CFR 565.10 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS VIN Requirements... vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  11. 49 CFR 565.10 - Purpose and scope.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS VIN Requirements... vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  12. 49 CFR 565.10 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS VIN Requirements... vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  13. 49 CFR 565.10 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS VIN Requirements... vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  14. 49 CFR 565.10 - Purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS VIN Requirements... vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  15. Problem identification for Virginia's highway safety plan.

    DOT National Transportation Integrated Search

    1982-01-01

    Problem identification is recognized as an important component of highway safety planning. Under the NHTSA/FHWA concept, problem identification is the first step in program planning and in the development of effective countermeasure programs. The ann...

  16. Yeast identification: reassessment of assimilation tests as sole universal identifiers.

    PubMed

    Spencer, J; Rawling, S; Stratford, M; Steels, H; Novodvorska, M; Archer, D B; Chandra, S

    2011-11-01

    To assess whether assimilation tests in isolation remain a valid method of identification of yeasts, when applied to a wide range of environmental and spoilage isolates. Seventy-one yeast strains were isolated from a soft drinks factory. These were identified using assimilation tests and by D1/D2 rDNA sequencing. When compared to sequencing, assimilation test identifications (MicroLog™) were 18·3% correct, a further 14·1% correct within the genus and 67·6% were incorrectly identified. The majority of the latter could be attributed to the rise in newly reported yeast species. Assimilation tests alone are unreliable as a universal means of yeast identification, because of numerous new species, variability of strains and increasing coincidence of assimilation profiles. Assimilation tests still have a useful role in the identification of common species, such as the majority of clinical isolates. It is probable, based on these results, that many yeast identifications reported in older literature are incorrect. This emphasizes the crucial need for accurate identification in present and future publications. © 2011 The Authors. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

  17. Investigating the effect of nurse-team communication on nurse turnover: relationships among communication processes, identification, and intent to leave.

    PubMed

    Apker, Julie; Propp, Kathleen M; Ford, Wendy S Zabava

    2009-03-01

    Enhanced team communication may strengthen nurses' attachment to their organizations and teams and improve nurse retention. This study examines the relationships among nurse-team communication, identification (organizational and team), and intent to leave. Hospital nurses (N = 201) completed surveys measuring 3 nurse-team communication processes: promoting team synergy, ensuring quality decisions, and individualizing communication. Hierarchical regression analyses revealed that promoting team synergy was a significant predictor of intent to leave, whereas ensuring quality decisions and individualizing communication did not account for significant additional variance in intent to leave. Separate analyses showed that the relationship between promoting team synergy and intent to leave was partially mediated by team identification or by organizational identification. Further analyses were conducted on the 7 communication practices for promoting team synergy. Mentoring emerged as the only significant predictor of intent to leave; however, its relationship to intent to leave was fully mediated by organizational identification or partially mediated by team identification. Pragmatic suggestions are offered to improve nurse identification and reduce turnover.

  18. A review of automatic patient identification options for public health care centers with restricted budgets.

    PubMed

    García-Betances, Rebeca I; Huerta, Mónica K

    2012-01-01

    A comparative review is presented of available technologies suitable for automatic reading of patient identification bracelet tags. Existing technologies' backgrounds, characteristics, advantages and disadvantages, are described in relation to their possible use by public health care centers with budgetary limitations. A comparative assessment is presented of suitable automatic identification systems based on graphic codes, both one- (1D) and two-dimensional (2D), printed on labels, as well as those based on radio frequency identification (RFID) tags. The analysis looks at the tradeoffs of these technologies to provide guidance to hospital administrator looking to deploy patient identification technology. The results suggest that affordable automatic patient identification systems can be easily and inexpensively implemented using 2D code printed on low cost bracelet labels, which can then be read and automatically decoded by ordinary mobile smart phones. Because of mobile smart phones' present versatility and ubiquity, the implantation and operation of 2D code, and especially Quick Response® (QR) Code, technology emerges as a very attractive alternative to automate the patients' identification processes in low-budget situations.

  19. A Review of Automatic Patient Identification Options for Public Health Care Centers with Restricted Budgets

    PubMed Central

    García-Betances, Rebeca I.; Huerta, Mónica K.

    2012-01-01

    A comparative review is presented of available technologies suitable for automatic reading of patient identification bracelet tags. Existing technologies’ backgrounds, characteristics, advantages and disadvantages, are described in relation to their possible use by public health care centers with budgetary limitations. A comparative assessment is presented of suitable automatic identification systems based on graphic codes, both one- (1D) and two-dimensional (2D), printed on labels, as well as those based on radio frequency identification (RFID) tags. The analysis looks at the tradeoffs of these technologies to provide guidance to hospital administrator looking to deploy patient identification technology. The results suggest that affordable automatic patient identification systems can be easily and inexpensively implemented using 2D code printed on low cost bracelet labels, which can then be read and automatically decoded by ordinary mobile smart phones. Because of mobile smart phones’ present versatility and ubiquity, the implantation and operation of 2D code, and especially Quick Response® (QR) Code, technology emerges as a very attractive alternative to automate the patients’ identification processes in low-budget situations. PMID:23569629

  20. What Happened to the Streptococci: Overview of Taxonomic and Nomenclature Changes

    PubMed Central

    Facklam, Richard

    2002-01-01

    Since the division of the Streptococcus genus into enterococci, lactococci, and streptococci in 1984, many changes in the nomenclature and taxonomy of the Streptococcus genus have taken place. The application of genetic comparisons has improved the proper classification of the different species. The Lancefield system of serogrouping the streptococci by the expression of beta-hemolysis on blood agar plates is still very useful for the identification of streptococci for patient management. The Lancefield grouping system cannot be used in itself for accurate identification of specific beta-hemolytic species, but it can be a useful part of the identification procedure. Except for identification of the “Streptococcus bovis group” of species and Streptococcus suis, Lancefield grouping is of little value in identification of the non-beta-hemolytic streptococci and related genera. In fact, identification of the non-beta-hemolytic species is problematic for conventional as well as commercially available identification procedures. A combination of conventional tests and specific chromogenic tests suggested by several investigators is presented and discussed. Tables are included that suggest tests and procedures to guide investigators attempting to identify all the species. PMID:12364372

  1. Nosewitness Identification: Effects of Lineup Size and Retention Interval.

    PubMed

    Alho, Laura; Soares, Sandra C; Costa, Liliana P; Pinto, Elisa; Ferreira, Jacqueline H T; Sorjonen, Kimmo; Silva, Carlos F; Olsson, Mats J

    2016-01-01

    Although canine identification of body odor (BO) has been widely used as forensic evidence, the concept of nosewitness identification by human observers was only recently put to the test. The results indicated that BOs associated with male characters in authentic crime videos could later be identified in BO lineup tests well above chance. To further evaluate nosewitness memory, we assessed the effects of lineup size (Experiment 1) and retention interval (Experiment 2), using a forced-choice memory test. The results showed that nosewitness identification works for all lineup sizes (3, 5, and 8 BOs), but that larger lineups compromise identification performance in similarity to observations from eye- and earwitness studies. Also in line with previous eye- and earwitness studies, but in disagreement with some studies on odor memory, Experiment 2 showed significant forgetting between shorter retention intervals (15 min) and longer retention intervals (1-week) using lineups of five BOs. Altogether this study shows that identification of BO in a forensic setting is possible and has limits and characteristics in line with witness identification through other sensory modalities.

  2. Nosewitness Identification: Effects of Lineup Size and Retention Interval

    PubMed Central

    Alho, Laura; Soares, Sandra C.; Costa, Liliana P.; Pinto, Elisa; Ferreira, Jacqueline H. T.; Sorjonen, Kimmo; Silva, Carlos F.; Olsson, Mats J.

    2016-01-01

    Although canine identification of body odor (BO) has been widely used as forensic evidence, the concept of nosewitness identification by human observers was only recently put to the test. The results indicated that BOs associated with male characters in authentic crime videos could later be identified in BO lineup tests well above chance. To further evaluate nosewitness memory, we assessed the effects of lineup size (Experiment 1) and retention interval (Experiment 2), using a forced-choice memory test. The results showed that nosewitness identification works for all lineup sizes (3, 5, and 8 BOs), but that larger lineups compromise identification performance in similarity to observations from eye- and earwitness studies. Also in line with previous eye- and earwitness studies, but in disagreement with some studies on odor memory, Experiment 2 showed significant forgetting between shorter retention intervals (15 min) and longer retention intervals (1-week) using lineups of five BOs. Altogether this study shows that identification of BO in a forensic setting is possible and has limits and characteristics in line with witness identification through other sensory modalities. PMID:27303317

  3. Identification and its vicissitudes as observed in children: a developmental approach.

    PubMed

    Kramer, S

    1986-01-01

    In the last thirty-five years, theories derived from observational studies on normally developing infants and toddlers as well as on children with severe pathology have augmented Freud's original contribution to the theory of identification. Mahler's formulations, in particular, are used in this paper to demonstrate the importance for identification of the mutual interaction between mother (or caregiver) and even the very young infant, and to delineate the gradual process by which are achieved intrapsychic self and object-representations. The thread of identification is followed from earliest mirroring of the young infant to post-oedipal ego identifications. I present aspects of the treatment of a borderline adopted boy to demonstrate problems in identification which occurs in part because his two primary caregivers competed to such a degree for possession of him, each demanding loyalty to herself and to herself alone, that his identity and identifications were confused, in fact duplicated. This white child had as his alter-ego, really as part of his self-representation, a black half of the self, personified as a black boy whom he fantasized to be his twin.

  4. A novel optimization algorithm for MIMO Hammerstein model identification under heavy-tailed noise.

    PubMed

    Jin, Qibing; Wang, Hehe; Su, Qixin; Jiang, Beiyan; Liu, Qie

    2018-01-01

    In this paper, we study the system identification of multi-input multi-output (MIMO) Hammerstein processes under the typical heavy-tailed noise. To the best of our knowledge, there is no general analytical method to solve this identification problem. Motivated by this, we propose a general identification method to solve this problem based on a Gaussian-Mixture Distribution intelligent optimization algorithm (GMDA). The nonlinear part of Hammerstein process is modeled by a Radial Basis Function (RBF) neural network, and the identification problem is converted to an optimization problem. To overcome the drawbacks of analytical identification method in the presence of heavy-tailed noise, a meta-heuristic optimization algorithm, Cuckoo search (CS) algorithm is used. To improve its performance for this identification problem, the Gaussian-mixture Distribution (GMD) and the GMD sequences are introduced to improve the performance of the standard CS algorithm. Numerical simulations for different MIMO Hammerstein models are carried out, and the simulation results verify the effectiveness of the proposed GMDA. Copyright © 2017 ISA. Published by Elsevier Ltd. All rights reserved.

  5. Development of adolescents' peer crowd identification in relation to changes in problem behaviors.

    PubMed

    Doornwaard, Suzan M; Branje, Susan; Meeus, Wim H J; ter Bogt, Tom F M

    2012-09-01

    This 5-wave longitudinal study, which included 1,313 Dutch adolescents, examined the development of peer crowd identification in relation to changes in problem behaviors. Adolescents from 2 age cohorts annually reported their identification with 7 peer crowds and their levels of internalizing and externalizing problem behaviors. Univariate latent growth curve analyses revealed declines (i.e., "Hip Hoppers" and "Metal Heads") or declines followed by stabilization (i.e., "Nonconformists") in identification with nonconventional crowds and increases (i.e., "Elites" and "Brains") or declines followed by stabilization (i.e., "Normals" and "Jocks") in identification with conventional crowds. Multivariate latent growth curve analyses indicated that stronger and more persistent identifications with nonconventional crowds were generally associated with more problem behaviors throughout adolescence. In contrast, stronger and more persistent identifications with conventional crowds were generally associated with fewer problem behaviors throughout adolescence with the notable exception of Brains, who showed a mixed pattern. Though characterized by fewer externalizing problems, this group did report more anxiety problems. These findings and their implications are discussed. PsycINFO Database Record (c) 2012 APA, all rights reserved.

  6. A Teaching Exercise for the Identification of Bacteria Using An Interactive Computer Program.

    ERIC Educational Resources Information Center

    Bryant, Trevor N.; Smith, John E.

    1979-01-01

    Describes an interactive Fortran computer program which provides an exercise in the identification of bacteria. Provides a way of enhancing a student's approach to systematic bacteriology and numerical identification procedures. (Author/MA)

  7. System Identification Methods for Aircraft Flight Control Development and Validation

    DOT National Transportation Integrated Search

    1995-10-01

    System-identification methods compose a mathematical model, or series of models, : from measurements of inputs and outputs of dynamic systems. This paper : discusses the use of frequency-domain system-identification methods for the : development and ...

  8. Electro-Optic Identification Research Program

    DTIC Science & Technology

    2002-04-01

    Electro - optic identification (EOID) sensors provide photographic quality images that can be used to identify mine-like contacts provided by long...tasks such as validating existing electro - optic models, development of performance metrics, and development of computer aided identification and

  9. 49 CFR 565.20 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS Alternative VIN... and physical requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of...

  10. 49 CFR 565.1 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS General... requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  11. 49 CFR 565.1 - Purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS General... requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  12. 49 CFR 565.20 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS Alternative VIN... and physical requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of...

  13. 49 CFR 565.1 - Purpose and scope.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS General... requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  14. 49 CFR 565.20 - Purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS Alternative VIN... and physical requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of...

  15. 49 CFR 565.20 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS Alternative VIN... and physical requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of...

  16. 49 CFR 565.1 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS General... requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  17. 49 CFR 565.20 - Purpose and scope.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS Alternative VIN... and physical requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of...

  18. 49 CFR 565.1 - Purpose and scope.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS General... requirements for a vehicle identification number (VIN) system and its installation to simplify vehicle identification information retrieval and to increase the accuracy and efficiency of vehicle recall campaigns. ...

  19. 40 CFR 62.9110 - Identification of sources.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Sulfuric Acid Mist from Existing Sulfuric Acid Plants § 62.9110 Identification of sources. (a) Identification of sources. The plan includes the following sulfuric acid production plants. (1) National Zinc Co...] Fluoride Emissions From Phosphate Fertilizer Plants ...

  20. 40 CFR 62.9110 - Identification of sources.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Sulfuric Acid Mist from Existing Sulfuric Acid Plants § 62.9110 Identification of sources. (a) Identification of sources. The plan includes the following sulfuric acid production plants. (1) National Zinc Co...] Fluoride Emissions From Phosphate Fertilizer Plants ...

  1. 48 CFR 211.275 - Passive radio frequency identification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Passive radio frequency identification. 211.275 Section 211.275 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... Requirements Documents 211.275 Passive radio frequency identification. ...

  2. A comparison of Heuristic method and Llewellyn’s rules for identification of redundant constraints

    NASA Astrophysics Data System (ADS)

    Estiningsih, Y.; Farikhin; Tjahjana, R. H.

    2018-03-01

    Important techniques in linear programming is modelling and solving practical optimization. Redundant constraints are consider for their effects on general linear programming problems. Identification and reduce redundant constraints are for avoidance of all the calculations associated when solving an associated linear programming problems. Many researchers have been proposed for identification redundant constraints. This paper a compararison of Heuristic method and Llewellyn’s rules for identification of redundant constraints.

  3. Dynamic Identification for Control of Large Space Structures

    NASA Technical Reports Server (NTRS)

    Ibrahim, S. R.

    1985-01-01

    This is a compilation of reports by the one author on one subject. It consists of the following five journal articles: (1) A Parametric Study of the Ibrahim Time Domain Modal Identification Algorithm; (2) Large Modal Survey Testing Using the Ibrahim Time Domain Identification Technique; (3) Computation of Normal Modes from Identified Complex Modes; (4) Dynamic Modeling of Structural from Measured Complex Modes; and (5) Time Domain Quasi-Linear Identification of Nonlinear Dynamic Systems.

  4. [Application of mtDNA polymorphism in species identification of sarcosaphagous insects].

    PubMed

    Li, Xiang; Cai, Ji-feng

    2011-04-01

    Species identification of sarcosaphagous insects is one of the important steps in forensic research based on the knowledge of entomology. Recent studies reveal that the application of molecular biology, especially the mtDNA sequences analysis, works well in the species identification of sarcosaphagous insects. The molecular biology characteristics, structures, polymorphism of mtDNA of sarcosaphagous insects, and the recent studies in species identification of sarcosaphagous insects are reviewed in this article.

  5. The Word Identification Test Battery: A New Approach to Mastery and the Assessment of Word Identification Skills. Report from the Project on Studies in Language: Reading and Communication.

    ERIC Educational Resources Information Center

    Johnson, Dale D.; And Others

    The work reported culminates research by the Project on the Assessment and Analysis of Word Identification Skills in Reading. The Word Identification Test battery was designed for elementary school children, with attention to the major issues pertaining to skills mastery and assessment that are raised in the review of mastery learning. Five…

  6. Auto identification technology and its impact on patient safety in the Operating Room of the Future.

    PubMed

    Egan, Marie T; Sandberg, Warren S

    2007-03-01

    Automatic identification technologies, such as bar coding and radio frequency identification, are ubiquitous in everyday life but virtually nonexistent in the operating room. User expectations, based on everyday experience with automatic identification technologies, have generated much anticipation that these systems will improve readiness, workflow, and safety in the operating room, with minimal training requirements. We report, in narrative form, a multi-year experience with various automatic identification technologies in the Operating Room of the Future Project at Massachusetts General Hospital. In each case, the additional human labor required to make these ;labor-saving' technologies function in the medical environment has proved to be their undoing. We conclude that while automatic identification technologies show promise, significant barriers to realizing their potential still exist. Nevertheless, overcoming these obstacles is necessary if the vision of an operating room of the future in which all processes are monitored, controlled, and optimized is to be achieved.

  7. Personal Identification by Keystroke Dynamics in Japanese Free Text Typing

    NASA Astrophysics Data System (ADS)

    Samura, Toshiharu; Nishimura, Haruhiko

    Biometrics is classified into verification and identification. Many researchers on the keystroke dynamics have treated the verification of a fixed short password which is used for the user login. In this research, we pay attention to the identification and investigate several characteristics of the keystroke dynamics in Japanese free text typing. We developed Web-based typing software in order to collect the keystroke data on the Local Area Network and performed experiments on a total of 112 subjects, from which three groups of typing level, the beginner's level and above, the normal level and above and the middle level and above were constructed. Based on the identification methods by the weighted Euclid distance and the neural network for the extracted feature indexes in Japanese texts, we evaluated identification performances for the three groups. As a result, high accuracy of personal identification was confirmed in both methods, in proportion to the typing level of the group.

  8. Recent development of mass spectrometry and proteomics applications in identification and typing of bacteria.

    PubMed

    Cheng, Keding; Chui, Huixia; Domish, Larissa; Hernandez, Drexler; Wang, Gehua

    2016-04-01

    Identification and typing of bacteria occupy a large fraction of time and work in clinical microbiology laboratories. With the certification of some MS platforms in recent years, more applications and tests of MS-based diagnosis methods for bacteria identification and typing have been created, not only on well-accepted MALDI-TOF-MS-based fingerprint matches, but also on solving the insufficiencies of MALDI-TOF-MS-based platforms and advancing the technology to areas such as targeted MS identification and typing of bacteria, bacterial toxin identification, antibiotics susceptibility/resistance tests, and MS-based diagnostic method development on unique bacteria such as Clostridium and Mycobacteria. This review summarizes the recent development in MS platforms and applications in bacteria identification and typing of common pathogenic bacteria. © 2016 The Authors. PROTEOMICS - Clinical Applications Published by WILEY-VCH Verlag GmbH & Co. KGaA.

  9. Network-assisted target identification for haploinsufficiency and homozygous profiling screens

    PubMed Central

    Wang, Sheng

    2017-01-01

    Chemical genomic screens have recently emerged as a systematic approach to drug discovery on a genome-wide scale. Drug target identification and elucidation of the mechanism of action (MoA) of hits from these noisy high-throughput screens remain difficult. Here, we present GIT (Genetic Interaction Network-Assisted Target Identification), a network analysis method for drug target identification in haploinsufficiency profiling (HIP) and homozygous profiling (HOP) screens. With the drug-induced phenotypic fitness defect of the deletion of a gene, GIT also incorporates the fitness defects of the gene’s neighbors in the genetic interaction network. On three genome-scale yeast chemical genomic screens, GIT substantially outperforms previous scoring methods on target identification on HIP and HOP assays, respectively. Finally, we showed that by combining HIP and HOP assays, GIT further boosts target identification and reveals potential drug’s mechanism of action. PMID:28574983

  10. Feel like you belong: on the bidirectional link between emotional fit and group identification in task groups

    PubMed Central

    Delvaux, Ellen; Meeussen, Loes; Mesquita, Batja

    2015-01-01

    Three studies investigated the association between members’ group identification and the emotional fit with their group. In the first study, a cross-sectional study in a large organization, we replicated earlier research by showing that group identification and emotional fit are positively associated, using a broader range of emotions and using profile correlations to measure group members’ emotional fit. In addition, in two longitudinal studies, where groups of students were followed at several time points during their collaboration on a project, we tested the directionality of the relationship between group identification and emotional fit. The results showed a bidirectional, positive link between group identification and emotional fit, such that group identification and emotional fit either mutually reinforce or mutually dampen each other over time. We discuss how these findings increase insights in group functioning and how they may be used to change group processes for better or worse. PMID:26300806

  11. Multiracial in Middle School: The Influence of Classmates and Friends on Changes in Racial Self-Identification.

    PubMed

    Echols, Leslie; Ivanich, Jerreed; Graham, Sandra

    2017-11-27

    In the present research, the influence of racial diversity among classmates and friends on changes in racial self-identification among multiracial youth was examined (n = 5,209; M age  = 10.56 years at the beginning of sixth grade). A novel individual-level measure of diversity among classmates based on participants' course schedules was utilized. The findings revealed that although there was some fluidity in multiracial identification at the beginning of middle school, changes in multiracial identification were more evident later in middle school. In addition, although diversity among classmates and friends both increased the likelihood of multiracial identification in the beginning of middle school, only diversity among friends mattered later in middle school, when fluidity in multiracial identification was at its peak. © 2017 The Authors. Child Development © 2017 Society for Research in Child Development, Inc.

  12. A comparison of microscopic and spectroscopic identification methods for analysis of microplastics in environmental samples.

    PubMed

    Song, Young Kyoung; Hong, Sang Hee; Jang, Mi; Han, Gi Myung; Rani, Manviri; Lee, Jongmyoung; Shim, Won Joon

    2015-04-15

    The analysis of microplastics in various environmental samples requires the identification of microplastics from natural materials. The identification technique lacks a standardized protocol. Herein, stereomicroscope and Fourier transform infrared spectroscope (FT-IR) identification methods for microplastics (<1mm) were compared using the same samples from the sea surface microlayer (SML) and beach sand. Fragmented microplastics were significantly (p<0.05) underestimated and fiber was significantly overestimated using the stereomicroscope both in the SML and beach samples. The total abundance by FT-IR was higher than by microscope both in the SML and beach samples, but they were not significantly (p>0.05) different. Depending on the number of samples and the microplastic size range of interest, the appropriate identification method should be determined; selecting a suitable identification method for microplastics is crucial for evaluating microplastic pollution. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Experimental studies of forensic odontology to aid in the identification process

    PubMed Central

    Saxena, Susmita; Sharma, Preeti; Gupta, Nitin

    2010-01-01

    The importance of dental identification is on the increase year after year. With the passage of time, the role of forensic odontology has increased as very often teeth and dental restorations are the only means of identification. Forensic odontology has played a key role in identification of persons in mass disasters (aviation, earthquakes, Tsunamis), in crime investigations, in ethnic studies, and in identification of decomposed and disfigured bodies like that of drowned persons, fire victims, and victims of motor vehicle accidents. The various methods employed in forensic odontology include tooth prints, radiographs, photographic study, rugoscopy, cheiloscopy and molecular methods. Investigative methods applied in forensic odontology are reasonably reliable, yet the shortcomings must be accounted for to make it a more meaningful and relevant procedure. This paper gives an overview of the various experimental studies to aid in the identification processes, discussing their feasibilities and limitations in day-to-day practice. PMID:21731343

  14. Three dimensional identification card and applications

    NASA Astrophysics Data System (ADS)

    Zhou, Changhe; Wang, Shaoqing; Li, Chao; Li, Hao; Liu, Zhao

    2016-10-01

    Three dimensional Identification Card, with its three-dimensional personal image displayed and stored for personal identification, is supposed be the advanced version of the present two-dimensional identification card in the future [1]. Three dimensional Identification Card means that there are three-dimensional optical techniques are used, the personal image on ID card is displayed to be three-dimensional, so we can see three dimensional personal face. The ID card also stores the three-dimensional face information in its inside electronics chip, which might be recorded by using two-channel cameras, and it can be displayed in computer as three-dimensional images for personal identification. Three-dimensional ID card might be one interesting direction to update the present two-dimensional card in the future. Three-dimension ID card might be widely used in airport custom, entrance of hotel, school, university, as passport for on-line banking, registration of on-line game, etc...

  15. ROC curve analyses of eyewitness identification decisions: An analysis of the recent debate.

    PubMed

    Rotello, Caren M; Chen, Tina

    2016-01-01

    How should the accuracy of eyewitness identification decisions be measured, so that best practices for identification can be determined? This fundamental question is under intense debate. One side advocates for continued use of a traditional measure of identification accuracy, known as the diagnosticity ratio , whereas the other side argues that receiver operating characteristic curves (ROCs) should be used instead because diagnosticity is confounded with response bias. Diagnosticity proponents have offered several criticisms of ROCs, which we show are either false or irrelevant to the assessment of eyewitness accuracy. We also show that, like diagnosticity, Bayesian measures of identification accuracy confound response bias with witnesses' ability to discriminate guilty from innocent suspects. ROCs are an essential tool for distinguishing memory-based processes from decisional aspects of a response; simulations of different possible identification tasks and response strategies show that they offer important constraints on theory development.

  16. Biased lineup instructions and face identification from video images.

    PubMed

    Thompson, W Burt; Johnson, Jaime

    2008-01-01

    Previous eyewitness memory research has shown that biased lineup instructions reduce identification accuracy, primarily by increasing false-positive identifications in target-absent lineups. Because some attempts at identification do not rely on a witness's memory of the perpetrator but instead involve matching photos to images on surveillance video, the authors investigated the effects of biased instructions on identification accuracy in a matching task. In Experiment 1, biased instructions did not affect the overall accuracy of participants who used video images as an identification aid, but nearly all correct decisions occurred with target-present photo spreads. Both biased and unbiased instructions resulted in high false-positive rates. In Experiment 2, which focused on video-photo matching accuracy with target-absent photo spreads, unbiased instructions led to more correct responses (i.e., fewer false positives). These findings suggest that investigators should not relax precautions against biased instructions when people attempt to match photos to an unfamiliar person recorded on video.

  17. Research on FBG-Based CFRP Structural Damage Identification Using BP Neural Network

    NASA Astrophysics Data System (ADS)

    Geng, Xiangyi; Lu, Shizeng; Jiang, Mingshun; Sui, Qingmei; Lv, Shanshan; Xiao, Hang; Jia, Yuxi; Jia, Lei

    2018-06-01

    A damage identification system of carbon fiber reinforced plastics (CFRP) structures is investigated using fiber Bragg grating (FBG) sensors and back propagation (BP) neural network. FBG sensors are applied to construct the sensing network to detect the structural dynamic response signals generated by active actuation. The damage identification model is built based on the BP neural network. The dynamic signal characteristics extracted by the Fourier transform are the inputs, and the damage states are the outputs of the model. Besides, damages are simulated by placing lumped masses with different weights instead of inducing real damages, which is confirmed to be feasible by finite element analysis (FEA). At last, the damage identification system is verified on a CFRP plate with 300 mm × 300 mm experimental area, with the accurate identification of varied damage states. The system provides a practical way for CFRP structural damage identification.

  18. Multi-look fusion identification: a paradigm shift from quality to quantity in data samples

    NASA Astrophysics Data System (ADS)

    Wong, S.

    2009-05-01

    A multi-look identification method known as score-level fusion is found to be capable of achieving very high identification accuracy, even when low quality target signatures are used. Analysis using measured ground vehicle radar signatures has shown that a 97% correct identification rate can be achieved using this multi-look fusion method; in contrast, only a 37% accuracy rate is obtained when single target signature input is used. The results suggest that quantity can be used to replace quality of the target data in improving identification accuracy. With the advent of sensor technology, a large amount of target signatures of marginal quality can be captured routinely. This quantity over quality approach allows maximum exploitation of the available data to improve the target identification performance and this could have the potential of being developed into a disruptive technology.

  19. System/observer/controller identification toolbox

    NASA Technical Reports Server (NTRS)

    Juang, Jer-Nan; Horta, Lucas G.; Phan, Minh

    1992-01-01

    System Identification is the process of constructing a mathematical model from input and output data for a system under testing, and characterizing the system uncertainties and measurement noises. The mathematical model structure can take various forms depending upon the intended use. The SYSTEM/OBSERVER/CONTROLLER IDENTIFICATION TOOLBOX (SOCIT) is a collection of functions, written in MATLAB language and expressed in M-files, that implements a variety of modern system identification techniques. For an open loop system, the central features of the SOCIT are functions for identification of a system model and its corresponding forward and backward observers directly from input and output data. The system and observers are represented by a discrete model. The identified model and observers may be used for controller design of linear systems as well as identification of modal parameters such as dampings, frequencies, and mode shapes. For a closed-loop system, an observer and its corresponding controller gain directly from input and output data.

  20. On the identification of continuous vibrating systems modelled by hyperbolic partial differential equations

    NASA Technical Reports Server (NTRS)

    Udwadia, F. E.; Garba, J. A.

    1983-01-01

    This paper deals with the identification of spatially varying parameters in systems of finite spatial extent which can be described by second order hyperbolic differential equations. Two questions have been addressed. The first deals with 'partial identification' and inquires into the possibility of retrieving all the eigenvalues of the system from response data obtained at one location x-asterisk epsilon (0, 1). The second deals with the identification of the distributed coefficients rho(x), a(x) and b(x). Sufficient conditions for unique identification of all the eigenvalues of the system are obtained, and conditions under which the coefficients can be uniquely identified using suitable response data obtained at one point in the spatial domain are determined. Application of the results and their usefulness is demonstrated in the identification of the properties of tall building structural systems subjected to dynamic load environments.

Top